LOCUS       DAOPQT010000001        15096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_4673_length_15096_cov_3.030783, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000001 DAOPQT010000000
VERSION     DAOPQT010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 15096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15096
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4673_length_15096_cov_3.030783"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..615
                     /locus_tag="PKG74_00005"
     CDS             145..615
                     /locus_tag="PKG74_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HOB89703.1"
                     /translation="MKDTYKLVISVVGCELAGIVGSAFTIPAIPTWYATLNKPTFSPP
                     NWIFGPVWTLLYFLMGLSFYLIWRKGWNNKEVKVAGKYFLAQLGLNFIWSPIFFGLKS
                     PALALLVIIAMWALIVMTIKKFHPLSKLASYLLIPYLLWVSFASILNAAIVILN"
     gene            861..1364
                     /locus_tag="PKG74_00010"
     CDS             861..1364
                     /locus_tag="PKG74_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89704.1"
                     /translation="MKKNILFSLLTLAMVLAPSAHASNSATVAATVLLENVSVSVSDG
                     TVDYGILALNSSNCTTTLTDTQTVTNEGNVNINILIRGQDSVNWTLSGSADTDKYVHR
                     FCNASVNDCDTPPTDYTALTLNNQTLGSALPPSDTIDLDLNLTTPTASTFFIQQDVDV
                     IITAIAS"
     gene            1372..1959
                     /locus_tag="PKG74_00015"
     CDS             1372..1959
                     /locus_tag="PKG74_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89705.1"
                     /translation="MKYVLFLWVILIAMIPDIAESRIGVGVGTSKIEITEILMPGEIY
                     NLPPLTVINTGDEVADYGVGISYHEQQPEIMPPESWFIFSPSKFTLQPGQSQPVSIKL
                     NIPIRTTPGDYFAYIEAHPIVSASGQTRINIASAAKIYFRIGTSNLVAAVYYKAASFW
                     NAYNPWTSRIAIAILIIGLIFIFKKFFKVQVNFKK"
     gene            2499..4070
                     /locus_tag="PKG74_00020"
     CDS             2499..4070
                     /locus_tag="PKG74_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89706.1"
                     /translation="MKRLFFICLVIAICYPALVLAIIKTNNPNFNGNANGWTLANGSG
                     TNTCGNTLSASEINFNSFAYSGTLGGQTAFRANGTSVILTINYRGMIHQPLTIPGTGN
                     VNVRGSFSYYGDEVPLLGILGSNASWIRLDLYNSSNTDFVANLGCVTLSDDVPWTTTS
                     PVNVNVPAGDYTIRATLRTSITGGLLLRTVTLGIDNINVNVAPSLLTLTQPATNVELG
                     WTASTPGTGAPGLHATQPYEIARKTSSGLTTSDIIATSGTNSYADSTALPNTTYWYAI
                     FDKDVNNNRSPISNEMSILTKPGVPQNVQVNAVDEESMEIDWDAPNEGAASYKIQRAP
                     DESGSPGTYADLKSGIVDTVYIDDGHACGDTYWYRVIAVNSSGESTPSSGVKGNTLYC
                     ISIVLNTDGSVDFGFMALESTKDSTPSGINDPETVTALNGAVDLDVKSTNFTDGINSW
                     SFGNGPGDKQTKFEFSNNGNNWTTFLVPQQYYMFKENVAKDATASIFLRLTTPLSSDS
                     LGPYNSNITIQASRP"
     gene            complement(4113..4559)
                     /locus_tag="PKG74_00025"
     CDS             complement(4113..4559)
                     /locus_tag="PKG74_00025"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOB89707.1"
                     /translation="MKKYPIVAIVVILKYKDRILLLKHNNGALKFPGGKMKLGESILE
                     TLQRELKEELDYSLKKEPELFSVFNYISKDKQEHTVFLDFIYPLSKKPKLFSPEKLEI
                     LWLTKEEIIANNITKDRKFLDKIFKYRRKSKASSFNKKLRPNLLLV"
     gene            complement(4747..5544)
                     /locus_tag="PKG74_00030"
     CDS             complement(4747..5544)
                     /locus_tag="PKG74_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOB89708.1"
                     /translation="MEQKTKNKYSPWILMIVALAIVATLFIYFTADKDKPNFSVSGHP
                     EWPPAMYRDKEEIAGIGAELMKIIGHDLGFNVEAKYTGQWSQTLEKARNSELDALVGI
                     YKTVERGSYFDFSTPYMLDPVAVYVKKGKSFNAPDWDSYIGKKGVLTTGDSYGQALDT
                     EMEQIFTTVRVDTVARAFDMLINDEADYFIYAYNAGDKALKEAGLTDRIEVLPRFIVV
                     EYLYFAFPKNSPMLEYLPQINEKLEQYKKDGTVDRLMAEYRSKFYNQ"
     gene            6281..6472
                     /locus_tag="PKG74_00035"
     CDS             6281..6472
                     /locus_tag="PKG74_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89709.1"
                     /translation="MGPAEQAFWQELFVEAKKAIEELNGRKPSGMPEKLKSLYDRALV
                     EFDGSKYCPIQQLKQFFSL"
     gene            complement(6534..7229)
                     /locus_tag="PKG74_00040"
     CDS             complement(6534..7229)
                     /locus_tag="PKG74_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOB89710.1"
                     /translation="MIFGGIQKTTLVDYPGKVACVLFTLGCDFKCSWCHNPELSSANL
                     IPQQPIIDQSEVFSFLESRKGLLQGICITGGEPTIQPDLIDFIRRVKEMGFLLKLDSN
                     GSHPETLEKLITGDLVDYIAMDIKNRQSQYEKTIGCPTDFYKVQKSIELVKTAPDYEF
                     RTTVVPGLVSEEDILEIAEIIDGSKRYYLQPFQSKKTAGFDYDKKSVLKKPDLEKIRD
                     KIKHRFDTCEVRG"
     gene            complement(7281..9407)
                     /locus_tag="PKG74_00045"
     CDS             complement(7281..9407)
                     /locus_tag="PKG74_00045"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOB89711.1"
                     /translation="MPPKKFRNHLTSVTKRDGETTPFDKTKIEQAIYKALTATDQGGI
                     KLAKQLADKVVRILNRRFKKKEAPHVEQIQDIVEEVLMLEDLTETAKAYILYREQRRA
                     IREAKTATDEASELIDSYLKEVDWEVKENSNMAFSLQGLNHYVASHVTKKYWLNKIYP
                     IEVRKAVADGEIHVHDLDILGPYCCGWDVGDVLLRGFGGVFGKIQSRPAKHLRTALGQ
                     LVNFFYTLQGESAGAQAFSNFDTYLAPFIRYDGLTFKEVKQCLQEFLFNCNVPTRVGF
                     QTPFSNISLDLVVPNHMKDMPIIIGGQHKEETYGSFQPEMDTFNKALCEVYVEGDATG
                     RVFTFPIPTYNITKDFDWSNPNYEGVWQMTAKYGIPYFSNFINSDMKPEDARSMCCRL
                     RLDNRELYKRGGGLFGANPLTGSIGVVTINMPRIGYLSKTKKEFLERLGRIMDISKTS
                     LEIKRKALEDLIEKGLYPYSRYYLSEAKKLRNSYWANHFSTIGLIGMNEALLNFLGVS
                     ISDKRGIKFAEEVLDFMRDRLSSYQEETGNLYNLEATPGEGTSYRLPLKDKKEYPDII
                     TAGEKDPYYTNSTQLPVNYTDDIFEAIGLQDGLQTKYTGGTVFHAFLGEALPDSEAVK
                     STIKTVFGKFRLPYFTLTPTFSICPSHGYIAGNHQFCPHCNTEQPCEIYSRVVGFLRP
                     IQQWNKGKREEFNQRKMFKVARAKQD"
     gene            complement(9537..10730)
                     /gene="ftsZ"
                     /locus_tag="PKG74_00050"
     CDS             complement(9537..10730)
                     /gene="ftsZ"
                     /locus_tag="PKG74_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOB89712.1"
                     /translation="MKKKTQKRKATSKQKKSRTVPVFVHIKVVGIGGGGGNAVTRMSE
                     DFIRGVEFVAINTDTQDLEYCEAKQKINIGRSLTKGMGTGMNPDLGRQAAEENRSDIA
                     EALQNTDLVFLTAGMGGGTGTGATPVVAEIAREMGILTVAIVTKPFAFEGSQRIRIAE
                     EGIAKLKDKVDSLIVIPNDRVFSIIGNDTSITKAFIKIDEILRNTVRGISEIVSIPGI
                     VNVDFADVKMIMGSGGLAFVGVGQAAGNDRAIKAATQAINSPLLEFSIDGAKGVLFGV
                     SGGADLKMAEINEAAKLVTENVDSGAKIIFGAYHDRKVKQGQLKIILIATGFNGLVSR
                     ERETFSLFSPLREEPMMKVSDPKKPEKEISEEKTGEEKSEIGKAAKADEEIWDIPTFL
                     RKKRK"
     gene            complement(10727..>11861)
                     /gene="ftsA"
                     /locus_tag="PKG74_00055"
     CDS             complement(10727..>11861)
                     /gene="ftsA"
                     /locus_tag="PKG74_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOB89713.1"
                     /translation="IAGFTRPSSGLRRGSVVEMDEVCKSVAEVLNEVKTFSRSSVKNI
                     FLNIGSVDVRSQVSRGIVAVARANSEIHEDDIERVVKASQAINIPQNRTILHTITKEY
                     VVDGVNDIRDPEGMIGARLEVSSMIIDAFAPALKNLQKCVEISGGSISGLVFNPLAAA
                     RSVLTKNQKELGVVLIDIGFGTTSLVIYEEDKILHTAIFPVGAGHITNDLAIALKIPV
                     ETAEKLKLAYGYALSKEVSSRDTIDLKKIDLTVTGSPSRKFIAEVIESRIQEILELVN
                     NELRLIGKSGRLPAGAVLCGGGAKMPGIVDLSRQELKLSTQIGLPQASYFELATNELA
                     EYLELPEWSVPLGLVLCSKDQGRVKSKPSGSLIVKIFQNLLPE"
     assembly_gap    11862..11871
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11966..12343)
                     /gene="ybeY"
                     /locus_tag="PKG74_00060"
     CDS             complement(11966..12343)
                     /gene="ybeY"
                     /locus_tag="PKG74_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOB89714.1"
                     /translation="MALEKRLQSLAEEALILMRRPDTSVSVYLISDSLMRELNFKYRR
                     KDKPTTVLSFIEPQIPHPETDKKVLGEIYLAPRYIEKNKEDIDRLLIHGLLHLLGYDH
                     TRKDDKIKMENKERDLARRLLEP"
     gene            complement(12343..12801)
                     /locus_tag="PKG74_00065"
     CDS             complement(12343..12801)
                     /locus_tag="PKG74_00065"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOB89715.1"
                     /translation="MAILPTIEEDLKSSMKNADTERVGILRLLMSAIHNEEIAKRGRG
                     GSGPLDPDEEIAVLKKEAKKRKESIELYRKGGRNDLAEKEERELKVINQYLPPEMSRE
                     AVEEKVKKVIAAGTKDFGLVMKEVMKELRGQVAGEVVAEVIKSLLEIESE"
     gene            complement(12806..13021)
                     /locus_tag="PKG74_00070"
     CDS             complement(12806..13021)
                     /locus_tag="PKG74_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89716.1"
                     /translation="MAIIVRKKEGESAGSLIFRFGKKIQQSGVIQETKKRRFKQRDAN
                     KRSKRVAALYKARKRKDIEKKKKLGLM"
     gene            complement(13025..13432)
                     /locus_tag="PKG74_00075"
     CDS             complement(13025..13432)
                     /locus_tag="PKG74_00075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOB89717.1"
                     /translation="MDCLFCKIAAKKISSEIIYEDEWVTAFLDVNPLTEGHTLIVPKK
                     HAENILDLDDNAIGPVFAAVKKVTAMLEKSLHPRGFTIGINHGKISGQSIDHLHIHVI
                     PRYTSDGGGSLHSVVHFPPTASLKETKNKILNA"
     gene            complement(13432..14763)
                     /gene="hisS"
                     /locus_tag="PKG74_00080"
     CDS             complement(13432..14763)
                     /gene="hisS"
                     /locus_tag="PKG74_00080"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOB89718.1"
                     /translation="MATSAKKTKSAKTAKSTVIQAPKGMHDILPEDAWYWEKVEQAVR
                     DTASFYGFDRIQTPILENADLFIRSVGESTDIVEKEMYVLKTRGGDHLALRPEMTAPV
                     MRAYIERGLSRVSQPLKVYYFGPAFRHENPQAGRYREFYQMGFETIGGESDPIYDAEV
                     IVAGSRFLESLKLKNLAIQVNSIGCKVCRPNYRKKLQDFYRKEMAATKKNKVVCKDCE
                     RRLETNPLRLLDCKDDLCVSLKAQAPSILDNLCFACRQHLKGVLEYLDEVALPYSLNP
                     YLVRGLDYYSRTVFEIFSDQSDLALMGGGRYDYLGEILSGKPIPAVGVAAGIERIIDV
                     IKTNNLLPLRKTTDKVFVIHVGDLAKKKAFALIEQFRKENIRVSAALGKDSLQAQLKA
                     ADKEAAPLSVIIGQKEVYEESVIIRDMKTGSQETVPLKKINDTLKEKLRKQ"
     gene            complement(14747..>15096)
                     /gene="lepB"
                     /locus_tag="PKG74_00085"
     CDS             complement(14747..>15096)
                     /gene="lepB"
                     /locus_tag="PKG74_00085"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOB89719.1"
                     /translation="FHAPTEEKQYYIKRVIGLPGETIVVENGEVFVNGVELKEEYLSQ
                     GMATRTIQQNEFTLGANQFFVLGDNRIMSFDSRNWGPLEREKIVGLVQLRLWPLQFLD
                     SLKEQSFSSGYLR"
BASE COUNT         3568 a         3748 c         3439 g         4331 t
ORIGIN      
        1 cacggcgacc gtaagaattt ttatattttt tgcgttattt gtcatatatt tatattactt
       61 tttcttttaa tcttcggcaa cagatttatc cgcatcgttc gatcgattgt cccgttttcc
      121 aaataagtcc ataatgtaga tatcatgaaa gacacatata aactcgttat ttccgtcgtc
      181 ggctgcgaat tggcgggcat tgtcggaagc gcgttcacca ttcctgctat tcccacgtgg
      241 tatgccacct tgaataaacc gacgttttct ccgccgaact ggatattcgg accggtctgg
      301 acgttgttgt atttcctgat ggggttatcc ttttacttaa tctggcgaaa aggctggaac
      361 aataaagagg ttaaggtcgc ggggaaatat tttctcgcgc aattgggtct taactttatt
      421 tggtcgccga ttttcttcgg cttgaaatct ccggcgcttg cattattggt gattatcgcc
      481 atgtgggctc ttatcgtcat gacgataaaa aagtttcatc ccctgtcgaa attggcatcc
      541 tacctcctta ttccttatct attgtgggtg agcttcgcct ccatattgaa tgcggcgatt
      601 gtcattttga attagaaagg aatccgaaaa gttatatcaa gcgatattta tcgcgcgaga
      661 tcgatgaccg gcattcggct ttaaacaagg cttattttat atccgacaaa aatatttatg
      721 accgtttgac agctctcccg aaacggattt tttatagtta tccacatccg ggctttaaaa
      781 aatcattgtc atcaatcttt tctttttgta tgatactgaa gatacttgtt ttttaacatt
      841 aaaaattaaa taaatttgtt atgaagaaaa atattctatt ttctcttctg acactcgcga
      901 tggtcttagc tccctcagct catgcttcga atagcgcaac ggtagctgcc acagttttat
      961 tagaaaatgt atcagtcagc gtttctgacg gcacagtaga ttacggtata cttgcgttaa
     1021 actcatcaaa ctgcacaacc actcttacag acacacagac tgtaaccaac gaaggcaacg
     1081 tcaatatcaa tattttgatt agaggccaag atagcgtcaa ttggacgctg tccggtagtg
     1141 cagacaccga taaatacgtt catcgttttt gcaatgcttc ggtcaatgat tgcgatactc
     1201 cacccaccga ttacacggct ctcaccctca acaaccagac attaggttcc gcccttccac
     1261 cgagcgacac aatcgatctc gacctgaatt taactactcc gaccgcttca actttcttta
     1321 ttcaacaaga tgtagatgtg atcatcacgg cgatcgcttc ataaaaacaa aatgaaatat
     1381 gttttgtttt tatgggttat tttgattgcc atgattcccg atatagcaga atcgcgcatc
     1441 ggtgtcggcg tcggaaccag taagatcgaa atcacggaga ttctgatgcc cggagagata
     1501 tataatctgc cgccgctgac cgtcatcaat accggagacg aagtcgccga ttacggagtc
     1561 ggtatttcct accacgaaca gcaacccgag ataatgccgc ctgaatcatg gtttattttc
     1621 tcgccatcca agttcactct tcaaccgggc caaagccagc cggtgtctat aaaactgaat
     1681 attcccatac gcacaacccc aggcgattat tttgcgtaca ttgaagccca cccgatagtt
     1741 tccgcatccg gacaaacaag aatcaacatc gcctcggcgg caaaaattta ctttcggatc
     1801 gggacgagta atttggtggc cgccgtatat tataaagccg cctctttttg gaacgcgtac
     1861 aacccttgga caagcagaat cgccatcgcg atcctgatca tcgggctcat tttcatattc
     1921 aagaagtttt tcaaggtgca agtgaatttt aaaaaatgat atggaagaca gtgaagccac
     1981 aatcaacgcg attgtagata taggtatctg cggaaacgac attatcgaag acgatgaaga
     2041 ttgcgaaggt tcgaatctgg gaaatcatac ctgtgcggac ttcgggttca gcggcggcac
     2101 cctttcttgc gacatatcgt gttctttcga cacttcgatg tgcttcggcg ctccgatacg
     2161 ctctatcggg cccatgggcc tcccggtgaa tcccgacgat gtcgtttctc ccgccgaccc
     2221 gattgatcta ttgtttttta gatcttttga cacatctccg agaacatcgg ccgcggattc
     2281 gccaatgtcg cgcctcgctc ccgtgatgtc gcgatacgga gcgagcaatc aaggcgcatc
     2341 cttgataacg tcgactgcgg ttcaactttt ttctccgatt aacagaaacg agacgcgtcc
     2401 cgatcgatgc tcatgcgcca tcgttgtttt tttttaacta tttttttggt tatcttgttt
     2461 ttgatctggt cttgtaaaag aaaatacaat actgatccat gaagcgcctg ttttttattt
     2521 gcttagtcat cgccatttgt tacccggctt tggttttggc catcataaag acgaacaacc
     2581 cgaattttaa cggcaatgcc aacggatgga cgctcgcgaa cggcagcggc acaaatactt
     2641 gcggcaacac cctttccgcc tccgaaatca acttcaatag ttttgcgtat tcgggaactt
     2701 taggcggcca aaccgctttc agggcgaatg ggacttcggt tatcctcacg atcaattacc
     2761 ggggtatgat acatcagccg ctgactattc ccggaaccgg caacgtaaat gtccgagggt
     2821 cgttcagtta ttacggcgac gaagtccctt tattgggtat attggggagt aatgcgagct
     2881 ggatcaggct cgatttatat aacagctcga ataccgattt tgtcgctaat ctgggttgcg
     2941 taactttgag cgacgatgtt ccgtggacaa ccacctcgcc cgtaaacgta aacgtgcccg
     3001 ccggcgacta tactatccgc gccactctga gaacgtcgat aaccggaggt cttctcttaa
     3061 ggacggtaac gctcggcatc gataacataa acgtgaatgt cgctccatcc ctactcaccc
     3121 tcacgcaacc ggcaacgaat gtcgagctcg gctggacagc gagtactccc ggaacaggag
     3181 cacccggact tcatgccacc caaccgtatg aaattgcccg caaaaccagc tccggtctta
     3241 ccacgagcga catcatcgcc acatccggca ccaacagcta cgccgattcc accgccctgc
     3301 cgaatacgac gtattggtat gcgatttttg ataaagatgt caacaacaat cgttctccca
     3361 tttccaatga aatgagtatt cttaccaagc cgggcgttcc ccaaaacgtc caagtgaacg
     3421 ccgtagacga agaaagcatg gaaatcgatt gggacgcgcc gaacgaaggc gccgccagtt
     3481 acaagataca acgagctccg gacgaaagcg gttctcccgg gacatatgcc gacctgaaat
     3541 cgggaatcgt ggacactgtc tacattgatg acggccacgc atgcggagat acgtattggt
     3601 accgcgttat tgcggtcaac tcttcaggcg aaagcacgcc atcgagcggt gtaaaaggca
     3661 acactttata ctgtatctct attgtcctta atacggatgg ctctgtcgat tttggattta
     3721 tggcgcttga atcgacgaaa gattcaacgc caagcggaat caacgacccg gaaaccgtca
     3781 ccgccctcaa cggtgcggtc gatctcgacg ttaaatcgac caattttacc gatgggataa
     3841 attcatggtc gttcggaaac ggacccggcg acaaacaaac caaatttgaa ttctcgaata
     3901 acggcaataa ctggacgact ttcctcgttc cacagcaata ctatatgttt aaagaaaatg
     3961 ttgccaaaga cgctaccgcc tccatattct tgcgtcttac cacgccgctt tcaagcgata
     4021 gtttgggacc atacaattct aatataacaa ttcaagcttc acgcccctaa aagcctatgg
     4081 gccgaattaa gacttttaag aatcttgaaa aatcaaacta gaagcaggtt gggtctcaat
     4141 tttttattga agctggaggc tttggatttt cttctatatt taaatatctt atcaagaaac
     4201 tttctgtctt tagtaatatt attagcaatt atttcttctt tggtgagcca taaaatctca
     4261 agtttttcgg gagaaaaaag ttttggtttt ttggataacg gataaataaa atcgagaaaa
     4321 acggtatgct cttgtttgtc tttggaaata taattgaaaa cactgaataa ttccggttcc
     4381 ttttttaaag aataatccaa ttcttcttta agttctcttt gtaaagtttc caagatcgat
     4441 tcccccagtt tcatttttcc gccggggaac tttaaagcgc cgttattatg ctttaacagc
     4501 aatatcctgt ctttatattt taaaataact acgatggcga cgattggata ttttttcatg
     4561 gcttgtaaaa gcctccgggg attcgtctaa aattatttgg ctcgcgtctt tgggcacatg
     4621 ataaattaaa atattaaatc gcaagactga tccgctccat taactatcca gaattcttta
     4681 tatttgcaat atttctattt ttatccttag cgttttctgg catttaaaga gcgctccgga
     4741 taaattttat tgattgtaaa atttactgcg gtattcggcc attaaccgat ccaccgttcc
     4801 gtctttttta tattgctcga gcttttcgtt tatctgaggc aaatattcaa gcattgggga
     4861 atttttcgga aaggcaaaat acaaatactc aacgacgata aaccttggta gaacttcgat
     4921 acggtccgtt aaacccgctt cctttaaagc tttatccccg gcattatacg catagataaa
     4981 gtagtcggct tcatcgttaa tcagcatatc aaaagctcga gccaccgtgt cgacacgaac
     5041 tgtcgtgaaa atttgctcca tttccgtatc aagcgcttgt ccgtatgaat ctccggtcgt
     5101 caacactcct tttttcccga tgtagctgtc ccaatccgga gcgttaaaac tttttccttt
     5161 tttgacatag accgcgacag gatcgagcat atagggagtg gaaaaatcaa aataacttcc
     5221 acgttcgact gttttataga ttcccacaag cgcgtccaat tcgctgtttc tagctttctc
     5281 cagggtctgg ctccattggc cggtatattt tgcttctacg ttaaagccta agtcatggcc
     5341 gattattttc atgagctccg ctcctatgcc ggcaatttct tctttatccc ggtacattgc
     5401 cgggggccat tccggatgtc cggatacgct aaaatttggc ttatctttgt cggctgtaaa
     5461 atagataaaa agtgttgcga caatagccaa cgccacaatc attaaaatcc aaggcgaata
     5521 tttatttttt gttttttgtt ccatattatt tcaataattc gttaatatcg tctgccattg
     5581 agaatattat agcatgtttt gttgcagtat aaacaacaaa aacacctctt gatttaagta
     5641 gcgtttttgt aatgaaacag ttaattttga gctaacacga gttggctccg acacctggat
     5701 tcgaaccagg aacccgctcc ttacacttaa tccaatgtta ccattggcgt ggactatatc
     5761 atttccgttt cgttgttaaa cgaagtacgg aatgaggcgc ttcgacccca ctttttttag
     5821 cggagtctac tctctttcga gatagtctct acacctgtcc cgcgatctgc gggaattggc
     5881 tcgggattat ccctttacgg gacttccccg aattcacccc attattcaat ctcgattact
     5941 cgagaaggct gcgtaatgcc ggcttcgatt tcgcgatgat aataattctt cgcgtctgtc
     6001 ggcatagatt ctcttatccg ccattgcctt gattatagca atatcggaaa gatggttcac
     6061 agggagacgc tctaccgttg agctatgtcg gaatattcat tttttaagcc gccatcttcg
     6121 ataagatagc gcctttataa tagtaaattc aactgttttt ttcaatagta tttgacataa
     6181 tcttttaaga tgttatcctg acgtatcttt ggaaggaggg agaaaagtgt gcgtagcggg
     6241 tccgatgggt cttcctctcg atgactatac gttagagaga atgggtccgg cagaacaggc
     6301 cttctggcaa gaactcttcg tggaagcaaa aaaagcgatc gaggagctca atggcagaaa
     6361 accctccggg atgccagaga agttaaagtc gctttacgac cgagcgctcg tcgagttcga
     6421 cggcagtaaa tactgcccga ttcagcaact taagcagttc ttttccctgt aactccctac
     6481 cgcccggtcg aagtaccttg ccgttcaata tgagcggcgt ttttttaatc taattatccc
     6541 cttacctcac aggtatcgaa cctgtgtttt attttatccc tgattttttc caaatccggc
     6601 ttttttaaaa cgcttttctt atcgtagtca aaacccgctg ttttcttgct ttggaacggc
     6661 tgaagataat atcttttcga gccgtcgata atttcggcga tctccaagat atcttcctcc
     6721 gagaccagac ccgggacaac cgttgtccgg aactcgtagt ccggagccgt ttttacgagc
     6781 tcgatgcttt tttggacttt ataaaagtct gtcgggcagc cgatggtttt ttcgtattgc
     6841 gattgccggt tcttgatatc catggcgatg taatccacca aatccccggt gatcagtttt
     6901 tccaaggtct ccggatgcga accgtttgaa tcgagtttta ataaaaatcc catttctttt
     6961 acccggcgaa taaaatcgat cagatccggc tggatagtcg gctctccgcc ggtgatgcaa
     7021 attccctgga gcaagccttt tctcgattcc aaaaaactga acacttccga ctgatcgatg
     7081 atcggctgct gtggaatcaa gttcgcagaa gaaagctccg gattgtgaca ccaagaacat
     7141 ttgaaatcgc accccagagt gaataaaacg caagccacct tgcccggata atcaactagg
     7201 gtcgtttttt ggatgccgcc gaaaatcata actaaagctt aacaaaagcc taagaaaatc
     7261 agaaatcggc cctggagagt ctaatcctgt ttggctctcg ccaccttgaa cattttccgc
     7321 tggttgaatt cctcgcgttt gcccttgttc cattgctgga tcggacgcag aaaaccgacc
     7381 accctcgagt agatttcgca gggctgttcg gtattgcaat gcggacaaaa ttgatggttt
     7441 ccggcgatgt atccgtggga cggacaaatg gaaaaagtcg gggtcagggt gaaatacggc
     7501 agccggaatt tcccgaaaac agtcttgatg gtcgatttca ccgcctcgct gtccggcaaa
     7561 gcttctccga gaaaggcgtg gaaaaccgtg ccgccggtat acttggtctg cagaccgtcc
     7621 tggagtccga tggcctcgaa aatatcgtcg gtgtaattga ccggaagctg ggtggagttg
     7681 gtgtaatacg gatccttttc tccggcggtg atgatatccg gatattcttt cttgtctttt
     7741 aaaggaagtc tgtaagaagt gccttctccc ggagtggcct ccagattgta aagattaccc
     7801 gtttcttcct ggtaagagct caatcgatcg cgcatgaaat cgagcacttc ctcggcgaac
     7861 ttgattcctc tcttatcgga aatactgacg cccaagaaat tcaaaagcgc ttcgttcatt
     7921 ccgatcagtc cgatcgtcga aaaatgattg gcccaatagg aattcctcag ttttttggct
     7981 tcactcaagt aataacgcga atacggataa agcccttttt cgatcaaatc ctccaaagcc
     8041 tttcttttta tctccaggct ggttttcgag atgtccatga tgcggccgag cctttccaaa
     8101 aactcttttt tggtcttcga aagatagccg atccgcggca tattgatggt gacgacgccg
     8161 atcgatccgg tcaaaggatt ggccccgaaa agtcctcccc cccgcttgta gagttcgcgg
     8221 ttgtccaggc gcaaacggca gcacatcgaa cgcgcgtctt cgggcttcat gtcggaattg
     8281 atgaaattcg agaaataagg aatgccgtat ttggcggtca tctgccagac gccttcgtag
     8341 ttcgggttcg accaatcgaa atccttggtg atgttgtagg tcggaatcgg aaaagtgaag
     8401 acccggccgg tggcgtcgcc ctcgacgtag acttcgcaaa gcgccttgtt gaaggtgtcc
     8461 atttccggct ggaatgaacc ataggtctct tctttgtgct ggccgccgat gattatcggc
     8521 atgtctttca tatggttcgg cacgaccaaa tcaagagaaa tattggaaaa cggagtctga
     8581 aatccgacgc gcgtcggcac gttgcaattg aataagaatt cctgaagaca ttgcttcact
     8641 tctttgaacg tcagaccatc gtaacgaata aagggcgcga gataggtatc gaaattggaa
     8701 aacgcctgcg cgccggcgga ttctccctgg agcgtataaa agaaattgac gagctgaccc
     8761 agagccgtgc gcaagtgttt ggccggacgc gactggattt ttccgaaaac tcctccgaat
     8821 ccgcgaagca aaacatctcc cacatcccag ccgcagcaat aaggacccag gatgtcgaga
     8881 tcgtgcacgt ggatctcgcc gtcggccacg gctttcctca cttcgatcgg gtaaatttta
     8941 ttcagccaat attttttggt gacgtgcgaa gcaacgtaat ggttcagtcc ttggagggaa
     9001 aaagccatgt tgctgttttc cttcacttcc caatcgactt ccttcagata gctgtcgatc
     9061 aattccgaag cttcgtcggt agcggtcttc gcttctctga tcgccctcct ttgttcgcgg
     9121 taaagaatgt aagccttggc ggtttccgtc agatcttcga gcatcaggac ctcctcgacg
     9181 atgtcttgaa tctgctcgac gtgcggagct tctttctttt tgaagcgccg gttcaaaatt
     9241 ctcaccactt tgtccgccaa ttgttttgct aatttgattc ctccctggtc ggtcgcggtc
     9301 aaagccttgt aaatggcttg ctcgattttg gtcttgtcga aaggagtcgt ttctccgtct
     9361 cttttcgtca cactcgtcag atgattccgg aacttttttg gcggcatgtt ttgctttgtt
     9421 tttgtattta tttttttgag gactttttaa gaatgtcctc taattttacc tcaaacaaaa
     9481 aaccggtcta tctccagaaa gccggttttc atgtggataa gtcgaagggt tttcttttat
     9541 ttgcgttttt ttctcaaaaa tgtcgggata tcccaaattt cttcgtccgc tttggcggct
     9601 ttgccaattt ccgacttttc ttcgcctgtt ttttcctcgg aaatctcttt ttccggcttt
     9661 ttggggtcgg aaaccttcat catcggctct tcccgaagcg ggctgaacag gctgaaagtc
     9721 tctctttcgc ggcttacgag gccgttgaag ccggtggcga taaggattat tttcaattgg
     9781 ccttgcttta ccttccgatc atgataggcg ccgaagatga tcttcgctcc ggaatcgacg
     9841 ttttccgtca ccagcttggc cgcttcgttg atttcggcca ttttgaggtc agcgccgccg
     9901 gaaaccccga aaagcactcc tttggcgccg tcgatcgaaa actccaaaag tggggaattg
     9961 atggcttgag tggccgcctt gatcgcccga tcgttgccgg ccgcctggcc aacgccgaca
    10021 aaagccaagc cgccgcttcc catgatcatt ttgacgtcgg cgaaatcgac gttgacgatt
    10081 cccgggatgg aaacgatctc ggaaatgcct ctgaccgtat ttcttaagat ctcgtcgatt
    10141 ttgataaaag ccttggtgat cgacgtatcg ttgccgatga tgctgaaaac ccgatcgttc
    10201 ggaatgacga tcaaagaatc gactttatct ttcagtttgg cgataccctc ttcggcgatc
    10261 ctgattctct gcgatccttc gaaggcgaaa ggcttggtga caatagccac ggtcaagatg
    10321 cccatttctc tcgcgatttc ggcgacgacc ggcgtggcgc cggtgcccgt gcctccgccc
    10381 atgccggcgg tcagaaacac cagatccgtg ttttggagag cctcggcgat gtccgaacga
    10441 ttttcctcgg ccgcctgcct gcccaaatcc ggattcatgc ccgtgcccat tcccttggtg
    10501 aggctccggc cgatgttgat tttttgtttg gcctcgcagt attccaaatc ctgggtatcg
    10561 gtgttgatgg ccacgaattc gactcccctg atgaagtctt cactcattct cgtcacggca
    10621 tttccgccac cgccgccgat gccgaccacc ttgatgtgga cgaaaaccgg aactgttctc
    10681 gatttttttt gtttggacgt tgcttttcgc ttctgcgttt tctttttcat tcgggtaaga
    10741 ggttctggaa tattttgacg atcaggctgc cgcttggctt ggattttacc ctgccctggt
    10801 ctttgctgca aaggaccaga cccaagggca cggaccattc cggaagctcg agatattcgg
    10861 cgagttcgtt cgtcgccaac tcgaaatacg aggcctgcgg caatccgatc tgggtggaaa
    10921 gcttgagttc ctggcgggac agatcgacga ttcccggcat tttcgctccg ccgccgcaaa
    10981 gaaccgctcc ggcaggcaga cggccggatt taccgatcag tcgaagctcg ttattcacca
    11041 actctaagat ctcctgaatc cgcgattcta tgacctcggc gatgaatttc ctggaaggcg
    11101 agccggtgac ggtcaaatct attttcttga gatcgatggt atcgcggctc gaaacctctt
    11161 tggaaagggc gtagccgtaa gcgagtttca gtttttcggc ggtttcgacc ggaattttca
    11221 aagcgatcgc caggtcgttc gtgatgtgtc ccgctcccac cggaaaaatg gccgtatgta
    11281 aaatcttgtc ctcctcgtag atgacaaggc tggtcgttcc gaaaccgata tcgatcaaaa
    11341 ccacgccgag ttctttctgg tttttggtaa gaaccgatct cgcggccgcc aaaggattga
    11401 aaaccaagcc gctgatgctt cccccggata tctccacgca tttttggaga ttcttcaggg
    11461 ccggcgcaaa agcgtcgatg atcatgctgg aaacttcgag ccgggcgccg atcatgcctt
    11521 cgggatcgcg aatatcgttc acgccgtcga ccacgtattc tttggtgatc gtatggagaa
    11581 tcgtccggtt ttggggaatg ttgatcgcct gcgaagcctt aaccacgcgc tcgatgtcgt
    11641 cttcgtggat ctccgaattg gcccgggcga cggcgacgat gccgcgggaa acctgggacc
    11701 tcacgtcgac gctaccaatg tttaaaaaaa tgtttttgac gctcgatcgg gaaaaagtct
    11761 tcacctcgtt taaaacttcg gccacggatt tgcagacttc atccatttcc acgaccgatc
    11821 cccggcgcag tccggaagaa ggccgggtga atccggcgat gnnnnnnnnn natgactttc
    11881 attttgtcgt tgcgggcttc ggctaagacc gccttgatat tggccgaccc gatatccaat
    11941 cctaaaaccg tgttttgagc catatttatg gttccaacaa tcggcgggcc aaatcccgct
    12001 ccttgttttc catttttatt ttatcatctt ttcgcgtatg gtcatacccc aaaagatgga
    12061 gcaacccgtg gatcagcaaa cggtcgatat cttctttgtt tttctcgata tatcgcggcg
    12121 cgagatagat ttcgcccaaa actttcttgt cggtttccgg atggggtatt tggggttcga
    12181 taaaggacaa tacggtcgtc ggcttgtctt tccgtcggta tttaaaatta agctcccgca
    12241 tcaggctgtc ggaaataaga taaacagaga ccgacgtatc cggtcggcgc attaagataa
    12301 gcgcttcctc ggccaaagat tgcagtcttt tttccaaggc cattattcgc tttcgatttc
    12361 gagcaatgat ttgatgactt ccgcgacaac ttctcccgcg acttgacccc ttagctcctt
    12421 catcacttct ttcatcacca atccgaaatc cttggttccg gcggcgatca cttttttcac
    12481 tttttcttcg acggcttcgc ggctcatttc cggaggcaga tactggttga tgactttgag
    12541 ttcgcgttct tctttttcgg ccaaatcgtt ccggcctcct ttacgataga gttcgatcga
    12601 ttcttttctt tttttggctt cttttttcaa aacagcgatt tcctcgtcgg gatcgagcgg
    12661 ccccgatcct ccgcggccgc gcttcgcgat ctcttcgttg tggatagcgg acattagtaa
    12721 acgcaaaatg ccgactcttt ccgtatcggc atttttcata cttgatttca aatcctcttc
    12781 tatggtcggc aaaatagcca tgggattaca tcaagccgag ttttttcttt ttttcgatat
    12841 cttttcgctt gcgcgctttg tagagcgcgg ccacccgctt ggaccgcttg ttggcgtccc
    12901 tctgtttgaa ccttcttttc ttggtttcct gaataacgcc cgactgctgg attttctttc
    12961 cgaaacggaa aatcagcgat ccggccgatt ccccttcttt ttttctgacg atgatggcca
    13021 ttgtttatgc gtttaaaatc ttgttctttg tttctttaag acttgcggtc ggcggaaaat
    13081 ggaccaccga gtggagcgac cctccgccgt ccgaagtata tctcggaatg acgtgaatat
    13141 gcagatgatc aatcgattgc ccgctgattt taccgtgatt tatgcctatt gtaaaccccc
    13201 ggggatgcag ggatttttcg agcatggcgg tcactttttt caccgccgcg aaaaccggtc
    13261 cgatggcgtt atcgtccaag tccaggatgt tttcggcatg ttttttggga acgatcaaag
    13321 tatggccttc ggttaaagga ttgacgtcca aaaaagccgt cacccactcg tcttcataga
    13381 tgatttctga cgagatcttc ttggccgcga ttttgcaaaa aagacaatcc attattgttt
    13441 ccgcaatttt tccttgaggg tatcgttgat ctttttcagg ggaacggttt cctgactgcc
    13501 ggttttcatg tcccggatga tgacgctttc ctcgtaaact tctttttggc cgatgatgac
    13561 gctcagcggc gcggcttctt tgtcggcggc cttgagctgg gcctgaagcg aatccttgcc
    13621 gagagccgcg gagaccctga tgttttcctt acggaactgc tcgatcaagg caaaagcctt
    13681 tttcttggcc agatcgccga cgtggataac aaaaacctta tccgttgttt tccgcaaagg
    13741 caagagattg ttagttttga tgacatcgat gatgcgctct atgcccgcgg ccacccctac
    13801 cgccggaatc ggcttgcccg aaagaatctc gccgaggtaa tcgtatcgtc cgccgcccat
    13861 tagggccaga tcgctttgat ccgagaaaat ctcgaaaacc gtgcggctgt aataatcgag
    13921 tccgcggacg agatacggat tcaggctgta gggcaaagcc acctcgtcca aatactcgag
    13981 aacgcctttc agatgctgcc tgcaggcgaa acaaagattg tcgagaatgc tcggcgcctg
    14041 ggctttcagg gaaacgcaaa ggtcgtcttt gcaatcgagc aagcgtaagg gattagtttc
    14101 caaacggcgt tcgcagtctt tgcaaacgac tttgtttttc ttcgtcgcgg ccatttcttt
    14161 ccgatagaaa tcctgaagtt ttttgcggta attcggccgg cagaccttgc agccgatgct
    14221 gttcacctgg atggccaggt ttttcagctt cagcgattcc aagaacctcg atccggcgac
    14281 gatcacttcg gcgtcgtaga tcggatcgga ttcgccgccg atcgtctcga atcccatttg
    14341 atagaattcg cggtagcggc cggcctgggg attttcgtgc ctgaaagccg gtccgaaata
    14401 ataaactttg agcggctggc tgacgcggct caatccgcgc tcgatatagg ctcgcatcac
    14461 cggagccgtc atttccggcc gcaaagccaa atggtcgccg ccgcgggttt tcagaacata
    14521 catttctttt tcgacgatgt cggtcgattc gccgacgctt cttataaaaa gatcggcgtt
    14581 ttcgaggatc ggtgtttgga tgcggtcgaa gccgtaaaac gaagccgtgt ctctcactgc
    14641 ctgctccacc ttttcccagt accaggcgtc ttccggcaga atgtcgtgca ttcctttcgg
    14701 agcttgaata accgtcgatt tggcggtttt cgcggatttg gtttttttag cggaggtagc
    14761 cactggaaaa gctttgttct ttcaacgaat ccaagaattg aagcggccaa agccgaagct
    14821 ggaccaatcc cactatcttt tccctctcga gcggacccca gttccgggaa tcgaagctca
    14881 tgatccggtt gtctccgagc acaaagaatt gatttgcgcc gagggtgaat tcgttttgtt
    14941 ggatagtcct cgtcgccatt ccctgggaaa gatattcttc ttttagctcg acgccgttca
    15001 cgaaaacttc tccgttttcg acgactatcg tctctccggg caagccgatg acccgcttga
    15061 tgtaatattg tttttcttcg gtcggagcgt ggaaaa
//
LOCUS       DAOPQT010000002         9844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_7829_length_9844_cov_3.307794, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000002 DAOPQT010000000
VERSION     DAOPQT010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7829_length_9844_cov_3.307794"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PKG74_00090"
     CDS             complement(<1..1024)
                     /locus_tag="PKG74_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOB89720.1"
                     /translation="MARLYPIEKNRDIGIIAHIDAGKTTVSERILFYTSVSHKLGEVH
                     EGQAIMDWMDQERERGITITSAATTCFWIPTYRKGDKAFEHRINLIDTPGHIDFTVEV
                     KRSLRVLDGAVVVFDGVSGVEPQSETNWHYADEYGVPRICFINKMDRMGASFDFVLRS
                     IHERLTPNAIPIQLPIGAESDFKGVIDLLQMKAFDFEGEHGEKVTEIPIPENMIEAAK
                     AARQTLLEKIVENDDKLMSDYLEGKEIGLEDLRRILRKAVLSLKIVPVVCGSALKNKG
                     VQLVLDAVIDYLPAPTDLPPVKGHDPKTNEEIERRPSDDEPFTALAFKIATDPFVGSL
                     TYFRVYS"
     gene            complement(1052..1516)
                     /gene="rpsG"
                     /locus_tag="PKG74_00095"
     CDS             complement(1052..1516)
                     /gene="rpsG"
                     /locus_tag="PKG74_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOB89721.1"
                     /translation="MRKKRNYKRERKPDVKYGDIAASRFINYLMYDGKKSVAEKNFYD
                     AMDIISKENPDPLAVFSKALENASPLLEVASKRVGGANYQVPREVREERKFMLACKWI
                     IDAARSKKGKPIAERLAEEIIAASKNEGAAIKKKLDIHRMAEANRAFAHFAR"
     gene            complement(1525..1938)
                     /gene="rpsL"
                     /locus_tag="PKG74_00100"
     CDS             complement(1525..1938)
                     /gene="rpsL"
                     /locus_tag="PKG74_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOB89722.1"
                     /translation="MPTIPQLLKKGRKTALKKDKTPALGRYMNVLQNKPVYSNSPFKR
                     GVCLKVSTVTPKKPNSALRKVARVRLTNGMEVTAYIPGEGHNLQEHSVVMIRGGRVKD
                     LPGVRYHIVRGRLDTAGMQRKQQRSKYGAKKPKAA"
     gene            complement(1999..2592)
                     /locus_tag="PKG74_00105"
     CDS             complement(1999..2592)
                     /locus_tag="PKG74_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOB89723.1"
                     /translation="MDILVLYVIIILSAVFHEYSHGLMALELGDPTARDSGRLTLNPI
                     PHLDMTGTVIVPLLSLFFFGSFIGWAKPVPVNPFLFKDQKNGILKVSVAGIAANMLLA
                     AVFGLFIRLGTGYFSPVAIMLFEQVVIVNISLGLFNLLPFPPLDGSKIFHELFPRAWT
                     NMMKLGAWGILLALFTAQIFLPAAGGLIFKLFTGQGY"
     gene            2615..2821
                     /locus_tag="PKG74_00110"
     CDS             2615..2821
                     /locus_tag="PKG74_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89724.1"
                     /translation="MANIYTMRQCAICGKGSILRSSRNKLRGKYNPTPLKRKYPNLQY
                     TRDANGKRVLACAKCIKAQAKKTK"
     gene            complement(2858..2928)
                     /locus_tag="PKG74_00115"
     tRNA            complement(2858..2928)
                     /locus_tag="PKG74_00115"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2894..2896),aa:Pro,seq:ggg)
     gene            complement(3169..3780)
                     /locus_tag="PKG74_00120"
     CDS             complement(3169..3780)
                     /locus_tag="PKG74_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOB89725.1"
                     /translation="MAEQIDKKERTLVVIKPDGVQRGLIGEVIKRYERSGLKLVGIKM
                     LVPTPEFTEKHYTLDPTWRERNGKKTIEAYKAKGLVPPSEDPLAVNAITLAKLKKYLS
                     SGPVVAMIWQGMHAAAVVRKITGSTEPLVSDVGTIRGDFTIDSYQAADEDGRITRNII
                     HASGSPEEAKMEVDLWFKPEELINYRLVTEEILYDVNLDGILE"
     gene            complement(3814..3984)
                     /locus_tag="PKG74_00125"
     CDS             complement(3814..3984)
                     /locus_tag="PKG74_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89726.1"
                     /translation="MIQPKSWFDSLGEWLTKVLEAAIKLLMILIVIAAYGILFWFGFI
                     VFREAIVYFQNL"
     gene            complement(3981..5393)
                     /gene="aspS"
                     /locus_tag="PKG74_00130"
     CDS             complement(3981..5393)
                     /gene="aspS"
                     /locus_tag="PKG74_00130"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOB89727.1"
                     /translation="MRILNKEAVSRIGEEVELKGWVHARRDHGKLVFIDLRDRSGLIQ
                     LVFTPDIPDYDKAQSLRLEWVIGIKGTVAARPETMVNPEIETGRIEIQVKELEVISEA
                     KTPPFEIDTDGRDISEDTRMKYRYLDLRQKRMADNMRNRNKIIRYMREYLAKKDFVEI
                     ETPIISKSTPEGARDYLVPSRVYPGKFYALPQSPQQYKQLLMVAGFERYFQIARCFRD
                     EDSRGDRQPEFTQLDIEMSFVDEKEVMALAEELYLGLVADLYPKKKVKLGPDGRIPVM
                     TYKEAMEKYGSDKPDIRDNKNDPDELAFLFVVDFPAFEWKETEKRWDAVHHPFTQPKV
                     KDVAEFKEMFAKDPAAIPAKQYDFVLNGYEIGGGSIRIHDPELLEAVFEAMGNDPEIV
                     RERFGHMLEAFRYGVPPHGGIAPGIDRFVMVMQNEPNIREVIAFPKTGDGRDLMMDAP
                     SEVDKSQLDELGFEIKKKTK"
     gene            complement(5406..5849)
                     /locus_tag="PKG74_00135"
     CDS             complement(5406..5849)
                     /locus_tag="PKG74_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89728.1"
                     /translation="MIGLEEGDRVVFKDSRIEVLSPGKEKALKDVNESSLVLLLDTGS
                     VKALFTGDIGMATEERITEKRDLSAEVLKVGHHGSKYSSAEEFLNRVRPAAAVIEVGK
                     NSYGHPTPEVVERLEKSGAAIYRTDLLGTIRVVFEKEGFKIMKIR"
     gene            complement(5859..6242)
                     /locus_tag="PKG74_00140"
     CDS             complement(5859..6242)
                     /locus_tag="PKG74_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOB89729.1"
                     /translation="MRKALLISALVLCNILVFREIYSLNAGLSEKQVYFLDVGQGDSE
                     LIRLQGANFLIDGGPDKKVLYELDRILEVSRRYLDVVFLSHPQADHLDGLREVFKSYD
                     IGIFIYAEAQENDRVLKEFLEHLFK"
     gene            complement(6202..7497)
                     /locus_tag="PKG74_00145"
     CDS             complement(6202..7497)
                     /locus_tag="PKG74_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOB89730.1"
                     /translation="MKPAEIFFSASLFFLAGVFLGSLDIGGIVILVLTAALSGGFLWF
                     ARRNGKWRTLAVLALFIPLGCFYFHFRESLSLPKIPLGEETTFVAKVIVEPESREKSQ
                     VLKLKLEPPWKGEVTSYNRLYPEYAYGDLLQVTGSIKESGYGGYSVSFPEIVSLGKDQ
                     GSTLMKTLFGLKSSLTSGLRKILPVSEAGFAIAAMFGDESLIDRGFADNLKKTGTSHL
                     IALSGYNVLIVVQGVQIFLSSLGLRRRSFVFSVLFVSGFVLMTGASASVMRAGIMAVL
                     SMIARKNSRFYSTKNTVAFTGLLMTLWNPRFLVFDLGFQLSFLAFFGVSCLAPFLKEK
                     LRLEDRGFCGWKENATQTLSAQIMVAPLLFFKTGGFSLLAIFPNILILPVMPMIMFFG
                     FVSAFASVVSRSLAVIFAFFLRILLSYAESVINLGARFM"
     gene            complement(7494..8537)
                     /gene="mnmA"
                     /locus_tag="PKG74_00150"
     CDS             complement(7494..8537)
                     /gene="mnmA"
                     /locus_tag="PKG74_00150"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOB89731.1"
                     /translation="MPRPGKKVFVAMSGGVDSSVSAYLLKTRGYDAVGVFMAGYNIDG
                     CDEKDSEDARRIAEKIGIPFYVWDFREEYERRVVDYMVEGYRSGLTPNPDVMCNKEIK
                     FGLFLERAKKLGADHIATGHYAVIDPKTKAVFAAKDKNKDQTYFLWAIKKEDLKHCLF
                     PVGGLLKSEVRAIAKKAGILAAEKKDSQGICFLGKVSIDDFLKLFIPPKQGPILDESG
                     KEIGRHEGAWYYTIGQRHGLGVPGKEPLYVAVKDTEKNIIVAVTAKSRLLFKDEFEIE
                     ETNWLVPEKELVDKNILCRVRYRQPLFEVQASLEKNKWFLRATKPQKFVAEGQSAVLY
                     DQKEKLLGGGIIK"
     gene            complement(8577..9149)
                     /locus_tag="PKG74_00155"
     CDS             complement(8577..9149)
                     /locus_tag="PKG74_00155"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOB89732.1"
                     /translation="MISYTNLKKGMIIRFNREIFEVIDVSFMRMQQRKAVVTTKLKNL
                     VSGKVVERTWQASDNIEEIDIERMEAVFLYSHRGEYWFQEKGNPKNRFMISEEVLGSS
                     GQFLKPQTEVIAFMLEMKPIKIQIPIKMELEVLEAPPSIKGNTAQGGAKMVTLQGGAK
                     INVPLFINQGDIIRVNTETGEYVERVEKTK"
     gene            complement(9151..>9844)
                     /locus_tag="PKG74_00160"
     CDS             complement(9151..>9844)
                     /locus_tag="PKG74_00160"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HOB89733.1"
                     /translation="MLITKLQQAAMSRADVPEAERRDFFLYIDEFQNFSTDSFAVVLS
                     EARKYRLSLILAHQYLEQLTDLVKAAIIGNVGTMIVFRVGAEDAEFLEKEFLPEFEDK
                     DLVNLSNWHAYIKMIIDGAPSRPFSIQNLPPALKPKESFSELIVEQSRKKHGTPKATV
                     EKQIAEEWTRREDVVEEKAERRSELPINQLLRPKERKPIIIDDLRKALSDALGSNATE
                     TSETKNTEETKQ"
BASE COUNT         2307 a         2585 c         2351 g         2601 t
ORIGIN      
        1 cgctgtaaac gcggaaataa gtgaggcttc cgacaaaagg atcggtggcg atcttgaaag
       61 ccaaagccgt gaaaggctcg tcgtcgctcg gtcggcgttc gatctcttcg ttggttttcg
      121 ggtcgtggcc tttgaccgga ggaagatcgg tcggcgccgg cagatagtcg ataaccgcgt
      181 cgagcaccaa ctggacacct ttgttcttca aagccgaacc gcagacgacc ggaacgatct
      241 ttaaagaaag gaccgccttt ctcaagatcc tccgcagatc ttcgagtccg atttcttttc
      301 cttcgagata atcggacatc aatttatcgt cgttctcgac gattttttcg agaagcgttt
      361 gtctcgccgc tttggcggct tcgatcatat tctcggggat gggaatctcc gtcacttttt
      421 ctccgtgttc gccttcgaaa tcaaaggctt tcatttgcaa aaggtcgatc actcccttga
      481 aatcgctttc ggcgccgatc ggcagctgga tcggtatggc gttcggagtg agcctctcgt
      541 ggatcgatct caaaacaaaa tcgaaactcg cgcccatgcg gtccatcttg ttgatgaaac
      601 aaatgcgcgg aaccccgtat tcgtcggcat agtgccagtt ggtctcggac tgcggctcca
      661 ctccggaaac gccgtcgaaa acaaccacgg cgccgtccaa aacccgaagc gaacgcttaa
      721 cttcgaccgt gaagtcgatg tgtcccgggg tgtcgatcag gtttatccgg tgctcgaaag
      781 ctttgtcgcc tttccggtaa gtcggaatcc aaaaacaggt ggtcgcggcc gaagtgatgg
      841 tgatgccgcg ttcgcgctcc tggtccatcc aatccatgat cgcctgacct tcgtggactt
      901 cgccgagttt gtgggaaacg ctggtgtaaa aaaggattct ttccgaaacc gtggtttttc
      961 cggcatcgat gtgggcgatg atgccgatat cgcggttttt ctcgattgga tagagtctag
     1021 ccataatagg ggtaaaaggt ggttgttaag gttatctcgc gaaatgcgcg aaagcccggt
     1081 tggcttcggc cattcggtga atgtccaatt ttttcttgat ggccgcccct tcgttcttgg
     1141 aagcggcgat tatttcttcg gccaagcgct cggcgatcgg tttacctttc ttggacctcg
     1201 cggcgtcaat gatccatttg caagccagca tgaatttcct ttcttcgcga acctcgcgcg
     1261 gcacctggta gttggcgccg ccgactctct tggaagcaac ctccaaaagc ggcgaagcgt
     1321 tttccaaagc tttcgagaaa accgccagcg gatccggatt ctccttggaa atgatatcca
     1381 tggcgtcgta aaagtttttc tcggcgacgg atttctttcc gtcgtacatc aaataattga
     1441 taaaccggct cgcggcgata tcgccgtact tcacgtccgg ttttctttcc cttttatagt
     1501 ttcttttttt cctcattgta taaattaggc ggctttgggt tttttggcgc cgtatttgct
     1561 cctttgctgt tttctttgca tgccggcggt atcgagcctg ccccggacaa tgtggtatct
     1621 cactcccgga aggtctttga cgcggccccc gcggatcatg acgaccgagt gttcctgcag
     1681 attatgccct tcgccgggaa tataggcggt cacttccatg ccgttcgtga gcctgaccct
     1741 cgccactttc cgcaaagcgg aattcggttt tttaggggtg accgtggaga ctttcaaaca
     1801 cactcctcgc ttgaaaggag aattggaata aaccggcttg ttttggagca cgttcatata
     1861 acgccccaag gccggggttt tatctttttt caaagcagtc ttcctgcctt ttttcaaaag
     1921 ttgtggaatt gtcggcacga cacctaatat accaagtcgt tcaagttaag tcaataatat
     1981 cgaggctttt ccggtcgttc aatacccttg gccggtgaag agtttgaaga tcaaacctcc
     2041 ggcggccggc aaaaaaatct gggctgtgaa aagcgcgagc aggatgcccc aagctccgag
     2101 cttcatcatg tttgtccagg cccgcgggaa aagctcgtgg aatattttcg agccgtcgag
     2161 cggcggaaac ggcaaaagat tgaacaaacc cagggaaata ttgacgatca ccacttgctc
     2221 gaaaagcatg atggcgaccg gagaaaagta ccccgttccc aaacggataa acaatccgaa
     2281 aacggccgcg aggagcatgt tggcggcgat accggcgacg cttactttca gaattccgtt
     2341 tttctggtct ttgaacaaaa acggattcac cggcaccggt ttggcccagc cgatgaaaga
     2401 accgaagaag aacaaagata aaagcggcac gatcaccgtt ccggtcatat cgagatgcgg
     2461 aatgggattc aaagtcagtc ggccggaatc ccgggcggtc gggtctccga gttcgagcgc
     2521 catgaggccg tggctgtatt cgtggaaaac cgccgaaaga atgatgataa cgtatagaac
     2581 cagaatatcc attggcttta cattataaac gtttttggct aatatttaca ccatgcgaca
     2641 atgcgcgatt tgcggcaaag gatcgattct ccgctcttcc aggaacaagc tccgcggcaa
     2701 atacaatccg actccgctca aaagaaaata tccgaatctt caatacaccc gagacgcgaa
     2761 cggcaaacgg gttttggctt gcgccaagtg catcaaagcc caggccaaga aaacaaaata
     2821 gaaacatccc gccgggcggg gtgttttttt attaatgtcg ggctgccgag aatcgaactc
     2881 ggtctacatg caccccatgc atgcgtacta ccggtatact acagcccgaa aaatgacgcc
     2941 ttaattttta ccacagtccc gaatcaaaaa caaaaatccc tctcctatga ttacccggcc
     3001 gaagccaagg tgggaatcct caatcctcgc ccacgggtga agatgctatc ggtttccccc
     3061 aaaaatatct ttgatccgga atcataagga gagggatcaa tctgattata ccacccggaa
     3121 tttatgttca agatcgcttt ccaaaaccgt tttgtggata agccgatcct attccagtat
     3181 tccgtccaaa ttgacgtcgt ataatatttc ttcggtcacc agacgatagt tgatcaactc
     3241 ttccggcttg aaccagagat cgacttccat cttcgcctct tcgggagagc cggaagcgtg
     3301 gatgatattt ctcgtgattc ttccgtcttc gtccgcggcc tggtacgaat cgatggtgaa
     3361 atcgccgcgg atggttccga catcggaaac caaaggctcg gtgctgccgg tgatcttccg
     3421 gacaacggcc gcggcatgca ttccctgcca gatcatggcc accaccggac cggaacttag
     3481 atattttttc aacttggcca aggtgatggc gtttacggcg agcggatctt ccgaaggcgg
     3541 aacaaggccc ttggccttgt aagcctcgat ggtctttttt ccgttccgtt cgcgccaggt
     3601 cggatccaag gtgtaatgct tttccgtgaa ttccggagtc ggaacgagca tcttgatccc
     3661 cacgagcttc aatcccgatc gctcataacg cttgataacc tcgccgatca atcccctttg
     3721 cacgccgtcc ggcttgataa cgacgagcgt tcgctctttt ttgtctatct gctccgccat
     3781 gattttattt ttagtttttt attaacgacg tttttaaaga ttttggaaat aaacgatcgc
     3841 ttcgcggaaa acgatgaacc cgaaccagaa aaggataccg taggcggcga tgacgatcaa
     3901 aatcatgaga agcttgatcg cggcctcgag gactttggtc agccattcgc ccaaactgtc
     3961 gaaccagctt tttggttgga tcattttgtt tttttcttga tttcgaaacc gagctcgtcg
     4021 agttgcgact tgtcgacttc cgaaggcgcg tccatcatca gatcccgacc gtctccggtt
     4081 ttcgggaaag caatcacctc cctgatgttc ggttcgttct gcatgaccat gacgaaccgg
     4141 tcgatgcccg gagcgattcc tccgtgcggc ggcaccccgt atcgaaaggc ctcaagcatg
     4201 tggccgaacc gctctctcac gatttccgga tcgttcccca tagcctcgaa aaccgcctcg
     4261 agtagttccg gatcgtggat tcttatggat ccgccgccga tttcgtagcc gttaaggacg
     4321 aaatcgtact gcttggcggg tatcgccgcc gggtctttcg cgaacatttc cttgaattcg
     4381 gcaacgtctt tcacttttgg ctgggtaaaa ggatgatgca ccgcgtccca gcgtttttcc
     4441 gtttctttcc actcgaaagc cggaaaatcg acaacgaaaa gaaacgccag ttcgtccgga
     4501 tcgttcttat tgtcacggat atccggtttg tcgcttccgt atttttccat cgcctccttg
     4561 taggtcatca cggggatgcg gccgtccgga ccgagtttta cttttttctt tggatagaga
     4621 tcagccacga gtcccaaata aagctcttcg gcgagagcca tcacttcttt ctcgtcgaca
     4681 aaactcatct cgatgtcgag ctgggtgaat tccggttggc ggtcgccgcg cgaatcctcg
     4741 tctcggaaac agcgggcgat ctggaaatat ctttcgaatc cggcgaccat caaaagctgt
     4801 ttgtactgct ggggagattg cggcaaagcg taaaatttcc ccggataaac gcgcgaaggc
     4861 acgagataat ccctcgcccc ttcaggcgtg gatttgctga tgatcggagt ttcgatctcc
     4921 acgaaatctt ttttggccag atattcccgc atgtagcgga tgatcttgtt gcggtttctc
     4981 atgttgtccg ccattctttt ctgccgcaga tcgagataac ggtatttcat cctcgtatct
     5041 tcgcttatgt cgcggccgtc ggtgtcaatc tcgaaaggcg gcgtcttggc ttcgctgatc
     5101 acttcgagct cttttacttg gatttctatc cggccggttt ctatttccgg attgaccatt
     5161 gtttccggtc ttgcagctac tgttcctttt attccgatca cccattcgag cctcaagctc
     5221 tgggctttgt cgtaatcggg gatatccggc gtgaaaacca attggatcaa tccgcttctg
     5281 tcccgaaggt cgataaaaac aagcttgccg tggtcgcgtc tcgcgtggac ccagcctttg
     5341 agttcgactt cttctccgat cctcgatacg gcttctttgt tcaaaatgcg cattgattca
     5401 gtatatcacc ggattttcat aattttaaag ccctctttct cgaaaaccac cctgatggtt
     5461 cccaagaggt ccgtgcggta gattgcggcg ccggatttct cgagccgctc cacaacttcc
     5521 ggagtcggat gaccatagga gtttttcccg acttcgatca cggccgcggc cggccggacc
     5581 cgattcaaaa attcttcggc cgaagaatac tttgatccgt ggtgcccgac tttcagcacc
     5641 tcggcggaaa gatcgcgttt ttccgtgatt ctttcttcgg ttgccatgcc tatatctccg
     5701 gtgaaaagcg ctttaaccga accagtatcg agcaaaagca ctaaagacga ttcattgacg
     5761 tcttttaatg ctttctcctt acccggagac agaacttcga tacgggaatc tttaaaaacg
     5821 accctatctc cttcttccaa accaatcacc ggaacacctt atttaaaaag atgttccaga
     5881 aattctttca agacccgatc gttttcctgc gcttcggcat agataaagat gcctatgtcg
     5941 tagcttttga aaacctccct taatccgtcc aaatgatccg cctgaggatg actcaaaaag
     6001 acgacgtcga gataacgcct cgaaacttcg aggattcggt cgagttcgta gagaactttc
     6061 ttgtccggtc ctccgtcgat caaaaagttc gcgccttgga gccttataag ttcgctgtct
     6121 ccctgaccca cgtccaaaaa atagacctgc ttttccgaca atcccgcgtt caaagaatag
     6181 atctcccgaa aaaccaggat attacataaa acgagcgccg agattaataa cgctttccgc
     6241 atagcttaat aatatccgaa gaaagaaagc gaagataacg gccaaagacc gtgaaaccac
     6301 cgacgcaaaa gcgctgacga atccgaaaaa catgatcatc ggcattacgg gcaagatcag
     6361 gatattcgga aaaatcgcta aaaggctgaa tcctccggtt ttgaagaaca aaagcggagc
     6421 gaccattatc tgggccgaaa gcgtctgggt ggcgttttct ttccaaccgc aaaatcccct
     6481 gtcttccaaa cgcagttttt ctttgagaaa aggcgcgagg caactcactc cgaaaaacgc
     6541 gagaaaagac agctgaaaac cgagatcgaa aaccaaaaaa cggggattcc aaagcgtcat
     6601 caagagtccg gtgaaagcga ccgtgttttt tgtagaataa aaacggctgt tctttctcgc
     6661 gatcatcgaa agcacggcca ttattccagc tctcatgacc gaagcactgg cgccggtcat
     6721 taaaacaaat cccgaaacga ataaaacaga aaatacaaaa ctccgacgcc tcaaaccgag
     6781 agaagacaag aaaatctgga ctccttggac aacgatcaag acgttgtatc cggataaggc
     6841 tataagatga ctggtgccgg ttttcttcaa gttgtccgca aaaccgcgat cgatcaggct
     6901 ttcgtcgccg aacatcgcgg cgatggcgaa acccgcctcg gaaaccggca agatttttcg
     6961 caaacccgat gtcaaagagc tcttcaatcc gaaaagcgtt ttcataagag tcgatccctg
     7021 gtctttaccg agcgaaacga tttccggaaa actgacgcta taaccgccgt aaccgctttc
     7081 ttttatcgaa cccgtcactt gaagcagatc accgtaggcg tattccggat acaggcgatt
     7141 gtagcttgta acctcgcctt tccagggagg ttcgagtttg agcttcaaga cttgagattt
     7201 ttcccttgat tccggttcaa cgatcacttt ggcgacgaat gtcgtttctt cgcctaaggg
     7261 aattttcggc aaagataaag attcgcggaa atgaaaataa aaacaaccga gaggaatgaa
     7321 taacgcgagt acggccaaag ttctccattt tccatttcgg cgagcaaacc acaaaaaacc
     7381 gccgctcaat gcggcggtca aaaccagaat cacgattccc ccgatatcta agctacctaa
     7441 gaaaacaccc gcgagaaaga aaagcgaggc gctgaaaaat atctccgctg gcttcatttg
     7501 attatccccc cgccgagcag tttttctttt tggtcgtata aaaccgcgga ctggccttca
     7561 gccacgaatt tctggggctt tgtcgcccgc aaaaaccatt tgttcttttc tagtgatgcc
     7621 tgaacttcaa aaagaggctg cctgtatctc acgcggcaga gaatgttttt atcgacgagt
     7681 tctttttccg gaaccagcca attcgtttct tcgatttcga attcgtcttt gaacaagagt
     7741 ctgctcttgg ccgtaacagc gacgatgatg tttttctccg tgtctttcac ggccacgtaa
     7801 agcggctctt tccccggaac tccaaggccg tgcctctggc cgatcgtgta ataccaggct
     7861 ccctcgtgcc gtccgatctc ttttcctgat tcgtcgagta tcggtccttg tttcggcgga
     7921 atgaaaagtt tcagaaaatc gtctatcgac acttttccca aaaaacaaat cccctgggaa
     7981 tctttctttt cggccgcgag aatgccggct tttttggcga ttgctctcac ttcggatttc
     8041 aaaagcccgc ccaccggaaa aagacagtgt ttcagatctt cttttttgat cgcccacaaa
     8101 aaatacgttt ggtccttgtt cttgtctttg gccgcgaaaa cagccttggt ctttggatcg
     8161 ataacggcgt aatggccggt ggcgatgtga tcggcgccga gctttttcgc tctttccaaa
     8221 aaaagaccga atttgatttc cttgttgcac atcacgtccg gattcggtgt gagaccactc
     8281 ctgtatccct cgaccatgta atccaccacc cggcgttcgt attcttcccg gaaatcccaa
     8341 acgtaaaaag ggatgcctat tttctcggcg atccttctcg cgtcctcact gtctttttcg
     8401 tcgcagccgt cgatgttgta gccggccatg aaaacgccga ccgcgtcgta gcctcgggtt
     8461 ttcagtaaat aggcggagac gctcgaatcc actccgccgg acatggcgac gaaaactttt
     8521 tttccgggac gaggcatttt atggaaatat tcgattgcgc ggatatgacg cgaggattat
     8581 ttcgttttct ccaccctctc gacgtattcg ccggtttcgg tgttcacgcg gatgatgtct
     8641 ccctgattga tgaaaagcgg aacattgatt ttggcgccgc cctgtaaagt caccattttg
     8701 gcgcctcctt gggcggtgtt gcccttgatc gaaggcggag cctcgagaac ttcgagttcc
     8761 atttttatcg gaatctggat cttgatcggc ttcatctcca gcatgaaagc aataacctcg
     8821 gtctgcggtt tcaagaattg tccgctcgaa ccgagaactt cctcggaaat cataaaacgg
     8881 tttttcgggt tgcctttttc ctggaaccaa tattctcccc tatggctgta aagaaaaacc
     8941 gcctccatcc gttcgatgtc gatttcttcg atattatccg acgcctgcca ggtgcgttcg
     9001 acgaccttgc cgcttacgag gtttttgagc ttggtcgtca ccaccgcttt tctttgttgc
     9061 attctcatga aagacacgtc gatcacttcg aatatctcgc ggttgaagcg gataatcatt
     9121 ccttttttta aattggtgta tgaaatcatg ttattgtttc gtctcttcgg tgtttttcgt
     9181 ctctgaggtt tcggtcgcgt tcgatcccaa agcgtcgctc aaggcctttc ttaaatcgtc
     9241 gatgatgata ggctttcttt ctttcggacg caagagctgg tttatcggca gctcgctccg
     9301 acgttcggct ttttcctcga caacgtcctc tcgcctggtc cactcttcgg cgatctgctt
     9361 ctcgacggtc gctttgggcg tgccgtgttt tttccgactc tgttcgacga taagttcgct
     9421 gaaggattcc ttaggcttca aagccggagg caagttctga atcgagaaag gtcgtgaagg
     9481 agctccgtcg atgatcattt tgatgtaggc gtgccagttg ctcaaattga ccaaatcctt
     9541 gtcttcgaat tccggcaaaa attctttttc caaaaattcg gcgtcttcgg cgccgacgcg
     9601 aaaaacgatc atcgtcccga cgttgccgat aatggccgcc ttgacgaggt cggtcagctg
     9661 ctccagatat tggtgcgcca ggatcaagct caagcggtat ttacgcgctt cggaaaggac
     9721 gacggcgaaa gaatccgtgg aaaaattctg gaactcgtcg atataaagga aaaagtcgcg
     9781 gcgttccgct tccggcacgt cggcccgact catggccgcc tgttggagtt tggtgatgag
     9841 catt
//
LOCUS       DAOPQT010000003         9336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_8343_length_9336_cov_3.191574, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000003 DAOPQT010000000
VERSION     DAOPQT010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 9336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9336
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8343_length_9336_cov_3.191574"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1828)
                     /locus_tag="PKG74_00165"
     CDS             complement(<1..1828)
                     /locus_tag="PKG74_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89734.1"
                     /translation="MNKKIISAAVLTALFTGVFNPPLALAWQNPTQNPPGGGGAFVIE
                     SGAPANSIYIKADGNVGIGATTTSAKLDVVGGDAKINGLTVGKGGGGKAGNTTLGYSA
                     LSVNTTGNSNTAVGMMALGFNTIGERNTALGYSALSANTTGSSNTAVGMQALSQNTIG
                     IFNTAVGDDALSVNTTGNSNTAVGYNALYSNTNSLNTAVGYNALYSNTTGNSNTAVGM
                     QALYSNTNSFNTAVGANALESNTTGANNTALGSGALTLNTTGANNTALGNEALLNNTT
                     ATNNTALGYYSLYSNKTGSNGVAIGTESQRYANDTTSVWTNYNTSVGYQSLRGGTTPA
                     SNTGNYNTALGYTTLYSNTSGANNTAVGYAAMDSNTTGLRNTAVGVNTIQAVTTATDN
                     AAIGYQALYANTGSNNTAVGSQALDSNTSGTRNTAVGKDSLQAVTTSSDVTAVGFDAL
                     YANTGASNTATGSYSLYSNTSAANNTAFGYYALGNLTTGANNTAAGYNAGRYAGSGTT
                     ANQTSANSIYLGYQARAGANGNTNETVIGYDVVGGGSNTVTLGNDSVTSTTLKGTVNI
                     PTLTVSGNANICNLVSYTGGGGTTQCPAGFYTWSGVALTSGYM"
     gene            complement(1916..2611)
                     /gene="rnc"
                     /locus_tag="PKG74_00170"
     CDS             complement(1916..2611)
                     /gene="rnc"
                     /locus_tag="PKG74_00170"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOB89735.1"
                     /translation="MDIISLENKIGVFFKNENILKEALTHRSYLNENPSWKLPHNERL
                     EFLGDAVLELVSTEYLWRVRPKFEEGLLTLLRAALVNTKMLSKIAEEEIGLQKYLLVS
                     KGESQMSGRAMETILADAFEALTGAIYVDQGFEAAKEFVSRFVLSRLEEIEKGQLYKD
                     PKSLLQEISQADFKVTPEYRILSESGPDHARIFTVGVYFGNELKGEGSGASKQEAETD
                     AAKKALEKLEAGK"
     gene            complement(2618..3109)
                     /gene="nusB"
                     /locus_tag="PKG74_00175"
     CDS             complement(2618..3109)
                     /gene="nusB"
                     /locus_tag="PKG74_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOB89736.1"
                     /translation="MSARQIGRSVALQSLYEWDFGGQKADLDAILDRNIKEFFPDSDD
                     KDFIKKLVSGVVHKMPDIDAIIEKAAPEWPINQIAPIDRNVLRMGLYELLFADRSEVP
                     PRVAINEAIELAKAYGGPNSGKFINGVLGTIYREIGEPDKGEPTKKEREAAGEDKRSA
                     EEK"
     gene            complement(3141..3290)
                     /gene="rpmF"
                     /locus_tag="PKG74_00180"
     CDS             complement(3141..3290)
                     /gene="rpmF"
                     /locus_tag="PKG74_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOB89737.1"
                     /translation="MPPVKHHSKSKVGRRRSHLAKTKTAMVACKKCGAPTMPHRACPS
                     CGYYR"
     gene            complement(3310..4017)
                     /locus_tag="PKG74_00185"
     CDS             complement(3310..4017)
                     /locus_tag="PKG74_00185"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOB89738.1"
                     /translation="MRYIIGIDEVGRGPLAGPVCVAACLLPADFKIKSGPSKLKDSKQ
                     LSAANRKKWRDWLCRESAKSKENKAAPLIYAMSRVYPRRIDETNINRAANAAALRSCR
                     KVLFELGRISPQEAEARVYLDGGLYLESRSDQEAMVGRDLEKIFIKSIRTIVAGDEKF
                     VAIKLASIVAKVSRDKYMDREHKKYPEYNFAKHKGYGTREHLEVVRRAGPCPIHRLTF
                     LKKYRNIRPEKGPFSGQ"
     gene            complement(4039..4599)
                     /locus_tag="PKG74_00190"
     CDS             complement(4039..4599)
                     /locus_tag="PKG74_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOB89739.1"
                     /translation="MAHSLGQKQRTAVYIDGSNFYHYLKNKQIGFHKGSFFDFEEFVS
                     YLVGGGRECVSKRYYTGIFRNIDNSDKSISLVSGQQKFLAKLENAGFIIKRGNIMYDA
                     GKPREKGTDVKIAVDLIVGAVDDEYDTAILISSDTDLIPALKYIRYKNKRIEYVGFSH
                     APSFGIQKNVDFSRLFLPEEIHKFIK"
     gene            4749..7892
                     /locus_tag="PKG74_00195"
     CDS             4749..7892
                     /locus_tag="PKG74_00195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOB89740.1"
                     /translation="MKFVHLHTHSHYSLLDGLSKIDDLVNYAKELGMEALAVTDHGNM
                     YGAVEFYKKAKAAGVKPIIGSELYVAFGSRFSKNPKVDNIRYHLTLLVKNEIGYKNLV
                     KLVTAANIEGFYYKPRIDHELIEKHHEGLVCLSGCFNGEIPRLLELGRTEEAKTVAMF
                     YKNLFAEDFYIEVQQHSGEIRPKLIKLANELQIPLVATQDSHYLRPEDKATHEVLLAI
                     QTQNHFDDEKRLTMKEFDLSLTSPDKMAELFADIPEALENTVKIAEKCVFEFELGKNK
                     LPAYPIPEGETSLSYLKKLIDERIGERFAEITPEIKERLDYEFSVIEQTGFASYLLIV
                     QDFVRWAKSHGIVVGPGRGSAASSIIVYILGITDVNPLPYGLLFERFLNPARIQMPDI
                     DMDFADTRREEVIGYVKEKYGDDHVAQIITFGTLAARAAIRDSGRALGYPYSFVDHIA
                     KLIPFEPNQSRSSHQLEDLLESNAEFRKEYEADPDAKKIIDAASKLEGVARHASVHAA
                     GVVITDKPITEYAPLQRSPQDETAYITQFEMHAIEDLGLLKMDFLGLRNLTIIEGCLR
                     LVKEKGDSIDINKLPLNDQDTFNLLQRGENIGVFQFEGAGMTRWLMAMKADRFEDLVA
                     MVALFRPGPMELIPSYIARKQGREPITYLHPKLEPILSGTYGIMIYQEQLLMIAREMA
                     GFTYGEADVLRKAVGKKIKSLLDEQAGKFVSGVEKTLGDKKLGEDLWKLAEPFARYGF
                     NKAHSVCYALIGYETAYLKAHYPQEFMTSLLNNDSSDVERIAVLVADCRRMKIQVLPP
                     DVNKSYSDFAPENQNIRFGLSAVKNVGVGITQAIVDERLRGGPFSNLEDFVSRINHKD
                     LNKKSLESLIKCGAMDSLSVERKTLLMNLDDIIRTLSAYRRSLAVSQTSLFGGAPKMT
                     IKLTPVAPATNMEKLNWEKELLGLFVSDHPLNGFKHNNDGVTPLKDVKNIRDGYNVTV
                     TGLVSKIQKINTKNGQPMLFVKLEDLSNNVEILVFNDTIQKHPDIWRENTILQVKGRV
                     SRKNGEPKIICESAKII"
     gene            7868..>9336
                     /gene="thrS"
                     /locus_tag="PKG74_00200"
     CDS             7868..>9336
                     /gene="thrS"
                     /locus_tag="PKG74_00200"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOB89741.1"
                     /translation="MRISKNNIKKPAGSKKAAGKPDLEAIRHSISHIVALATLERFPK
                     AKLGIGPTIENGFYYDFGIKEISEDNLKAIEGRVRELLKQGLKFKKEIVTPTEARKIF
                     KDQPFKLELVDELRKAKKPLSVYKTYDKSLKNPLFIDLCAGPHVKSTAEIDPEAFKLT
                     SIAGAYWRGNEKNPMLQRIYGLAFSNKKELDDYLRLLEEAEKRDHKKLGKELGIFFID
                     ETAPGMPYWLPKGTIMVNELLKFWREEHKKRGYLETITPLLNKKQLYETSGHWDHYRE
                     NMFISDMGEDGVYCLKPMNCPNAMTIFKQETHSYRELPLRLSDCDTLHRHEKSGELNG
                     LFRVQKFAQDDAHIFVTEEQISDEFKRILEIANLFYSIFDIKFKIRLGTKPDDYMGDD
                     KTWARAEKELEAILKKNKFDYFIGQGEGAFYGPKLDILMQDSLGRQWQTGTIQLDFQL
                     PRKFDLKYTDKDGKEKTPAVIHRVIYGSLERFMGILIEH"
BASE COUNT         2480 a         2304 c         2265 g         2287 t
ORIGIN      
        1 gcatgtatcc ggaagttaaa gcgacaccag accaggtata aaagccagcg ggacactggg
       61 tggtgccgcc gcctccggta taggaaacta ggttgcagat gttggcgttg ccggagacgg
      121 tcagggtagg aatattaaca gttcctttta aagtcgtgct cgtaacacta tcattgccca
      181 gagtgacggt atttgaaccg cccccgacga cgtcataacc aatgactgtt tcattggtat
      241 taccgttggc cccggctcta gcttgatatc ctaaatagat agaattagcg gaggtctggt
      301 tggccgtagt gccgctaccc gcatatctac ccgcattata accagcggcg gtgttattgg
      361 ctccggttgt taagttccca agagcataat aaccaaaagc agtgttgttg gcggcggaag
      421 tattggaata aagggaataa gaaccggtag cggtgttact ggcccccgta tttgcgtata
      481 aggcgtcgaa tcctacagcg gtaacatcgc tggatgtcgt aacggcttgt aaagaatctt
      541 tgccaacagc ggtattgcgg gtgccactcg tattgctatc caaagcttgg gagccgacgg
      601 cggtgttgtt ggaacccgta ttggcatata gggcttggta cccaatagca gcattatcgg
      661 tggcagttgt tacggcttgt atggtattaa cgccgacagc ggtgttgcgg agcccggttg
      721 tgttgctatc catggcagcg taaccaacag cagtattatt ggcaccggat gtattggaat
      781 aaagagttgt ataaccaaga gcggtgttgt aatttccggt gttactggca ggggtcgtgc
      841 ctcctcggag cgattgatag ccgaccgagg tattataatt tgtccaaaca gaggtagtat
      901 cgttcgcgta tcgttgagat tcagtgccaa tagcaacacc gttactaccg gttttattgg
      961 aataaagaga ataataacct agggcggtgt tgttggttgc ggttgtattg tttaaaagag
     1021 cctcattacc aagagcggtg ttattagcgc cagttgtgtt gagagtaagg gcgccactac
     1081 caagagcggt gttattagcg ccagttgtgt tagactcaag ggcgttcgca ccaacagcgg
     1141 tgttaaaaga attcgtgttg gaataaaggg cttgcatccc aacggcagtg ttactattac
     1201 cagttgtgtt ggaataaagg gcgttataac caacggcagt gtttaaagaa tttgtgttgg
     1261 aataaagggc gttataacca acggcagtgt tactattacc agttgtgttg acagaaaggg
     1321 cgtcatcacc aacggcggtg ttaaaaatgc caattgtgtt ttgagaaagg gcttgcatac
     1381 caacggcggt gttactacta ccagttgtgt tggcagaaag ggcggaataa ccaagggcgg
     1441 tgtttctctc tccaattgtg ttgaaaccaa gggccatcat cccaacggcg gtgttactat
     1501 taccagttgt gttgacagaa agggcggaat aacccagagt cgtattacca gccttacctc
     1561 ctcctccctt tcccaccgtt aatccattta tcttcgcgtc acctccaact acatcgagtt
     1621 tggcgctagt ggtggtcgct ccgatgccga cgtttccatc ggctttgatg tagatggagt
     1681 tcgccggagc gccggactcg atcacaaaag ctcctccgcc acctggggga ttttgggttg
     1741 gattttgcca agccaaagcc agcggcgggt taaaaactcc ggtaaacaaa gcggttaaga
     1801 cagccgcgga gatgattttt ttgttcatat ctttatttat ataaagacga attcttgaat
     1861 aaattataca ccaaaaaccc aaccacagcg tggggataag tacgttcccg taagtttatt
     1921 ttccggcctc caatttttcc agcgcttttt tagccgcgtc ggtttcggcc tcctgtttcg
     1981 aggcgccgga accttctcct ttcagttcgt ttccgaaata aacgccgacc gtaaagatcc
     2041 gcgcgtgatc cggcccggat tcggataaaa tcctgtattc cggcgtcacc ttgaagtcgg
     2101 cctggcttat ctcctggagc aggcttttcg gatctttgta aagctgtcct ttttcgattt
     2161 cctcgagacg ggagagtacg aatcgggaaa cgaattcttt ggccgcctca aaaccctgat
     2221 ccacgtaaat agcgccggtc aaggcttcga aagcgtcggc caagatcgtt tccatggccc
     2281 ggccggacat ttgggattcg cctttggaaa ccagaaggta tttttggagt ccgatttctt
     2341 cttcggcgat tttggaaagc attttggtgt tcaccaaagc ggcccgaaga agagtaagca
     2401 atccttcttc gaatttcggc cgcactctcc aaagatattc ggtggagaca agctccaaaa
     2461 cagcgtctcc taaaaattcc agcctttcgt tatggggaag tttccacgaa ggattctcgt
     2521 tgagatagga acggtgagtc agggcttctt tcagaatatt ctcgtttttg aagaaaactc
     2581 cgattttatt ttccaaagaa atgatgtcca cgtgcggtta tttttcttcc gcggatcttt
     2641 tgtcttcgcc ggcggcttcc ctttcttttt tggtcggctc gcccttgtct ggttcaccga
     2701 tctcccggta aatggttccc aaaacaccgt tgatgaattt tccggaattc ggaccgccgt
     2761 aagctttggc aagctcgatc gcttcgttaa tggccaccct cggcggcact tcgctccggt
     2821 cggcgaaaag aagttcgtac aaacccattc tcaaaacatt acggtcaatg ggagcgatct
     2881 ggttgatcgg ccattccgga gcggctttct caatgatggc gtcgatatcc ggcattttat
     2941 ggaccacgcc cgatactagc ttcttaataa aatctttgtc gtcggaatca ggaaaaaact
     3001 ccttgatatt cctatcaagg atggcgtcga gatcggcttt ctggccgccg aaatcccatt
     3061 cgtagagcga ttgtagagcg actgatcttc ctatttggcg ggcggacacg ttggttaaaa
     3121 attaaaaaag aaaaggccgg ttatctgtaa tacccgcaac tggggcaagc ccgatggggc
     3181 attgtcggag ctccgcattt cttgcaggcg accatggcgg ttttggtctt cgcgagatgc
     3241 gagcgccggc ggccgacttt ggatttcgaa tgatgtttta ccggaggcat gtttttatga
     3301 ttacggtgtt tattgacccg aaaacgggcc tttttcaggc cttatgttac ggtatttttt
     3361 cagaaacgtc aatcgatgaa taggacaagg accggctctc cggacgacct cgagatgctc
     3421 tcttgtaccg tatcctttgt gtttcgcgaa attgtattcc ggatatttct tgtgctcccg
     3481 atccatgtat ttatcccgag aaactttggc gacgatcgaa gccagtttga tggctacgaa
     3541 tttctcgtct ccggcgacga tcgtcctgat cgattttata aagatctttt ccaaatcccg
     3601 gccgaccatc gcctcttgat ccgatcggct ttctagatac agtccgccgt cgagataaac
     3661 ccgcgcttcc gcttcttgcg gcgagatccg gccgagttca aacaagactt ttctgcatga
     3721 tctcaaggcc gccgcgttgg ccgcgcgatt gatgttcgtc tcgtcgatcc gccggggata
     3781 gaccctgctc atcgcgtaaa tcaaaggcgc cgctttattt tctttgcttt tcgcggattc
     3841 gcggcagagc cagtcgcgcc attttttcct gtttgcggcc gaaagttgtt tcgagtcttt
     3901 gagtttcgac ggcccggatt ttattttgaa gtcggccggc aaaagacaag cggcgacgca
     3961 aaccggaccg gccaaaggcc cgcgtccgac ttcgtcgatg ccgataatat agcgcataaa
     4021 aagaagcgtt gggaacgatt atttaataaa tttatgaatt tcttcgggta aaaataacct
     4081 agaaaaatca acgttctttt gaattccaaa agagggtgcg tgagaaaacc caacatattc
     4141 gattctcttg tttttatatc taatatattt caaagccggt attaaatcgg tatcggagct
     4201 gatcaatata gccgtatcgt attcgtcatc gaccgcaccc acgatcaaat ccactgctat
     4261 tttgacatcg gttccttttt ctcgcggttt acccgcgtca tacataatat ttcctctttt
     4321 aataatgaaa ccggcatttt ctaatttagc cagaaatttc tgctgtcccg aaacaagaga
     4381 aatactttta tcggaattat caatattcct aaaaatcccc gtgtaatatc ttttggaaac
     4441 gcattctctt ccgccaccga ccaaataaga aacgaactcc tcgaaatcga aaaaacttcc
     4501 tttgtgaaat cctatctgtt tatttttaag gtagtgataa aaattactac cgtctatata
     4561 aacagcggtt ctttgttttt gaccgagcga gtgcgccata cagaaattct ataacaaaaa
     4621 ctgcccttgc cgataggcag gagcagtgat catattaaat atatgataag cattgtttat
     4681 cttcttgtca acacccggac aaatctccca gtttggagcc tatagtcaat cgctttataa
     4741 ttataccaat gaaattcgtc caccttcata cccacagcca ttactcgcta ttggacgggt
     4801 tgtcgaaaat cgacgatctc gtgaactacg ccaaagaact ggggatggag gctttggccg
     4861 tcaccgacca cggcaacatg tacggagccg tggagttcta caaaaaagcc aaagccgcgg
     4921 gagtaaagcc gattattggc tcggagctct acgtcgcctt cggatcgaga ttctccaaaa
     4981 accccaaagt ggacaatatc cgctatcacc tcaccctgct cgtcaagaac gaaatcggat
     5041 acaaaaacct cgtgaagctg gtcacggcgg cgaacatcga agggttttat tacaaaccgc
     5101 gcatcgacca cgaattgatc gagaaacacc atgaagggct ggtttgcctt tccggatgct
     5161 tcaacggaga aatcccgagg ctactcgagc tcggtcgaac cgaagaggcc aaaaccgtgg
     5221 ccatgttcta caagaacctt ttcgccgaag atttttacat cgaagtccag cagcattcgg
     5281 gcgagattcg gccgaaactc atcaaactgg cgaacgaact tcaaattccc ctggtcgcca
     5341 cccaggattc ccactatctc cggcccgaag acaaggccac ccacgaagtg cttctcgcca
     5401 tccaaaccca gaaccatttc gacgacgaaa aaagattgac gatgaaggaa ttcgatcttt
     5461 ctttaacctc gccggacaaa atggccgaac ttttcgcgga cattcccgaa gccctggaaa
     5521 ataccgttaa aatcgccgaa aaatgcgttt ttgaattcga gcttggaaaa aataagctgc
     5581 cggcttatcc cattcccgaa ggagaaacca gtctttctta tttgaaaaag ctgatcgacg
     5641 agagaatcgg cgaacggttt gcggagatca cgccggaaat aaaagaaagg cttgattacg
     5701 aattctcggt catcgaacaa accggcttcg caagctactt gctgattgtc caggactttg
     5761 tgcgctgggc caaaagccac ggcattgtcg ttggtccggg cagaggttcg gccgcttcga
     5821 gcattatcgt ttatatcctt gggatcaccg acgtcaatcc cttgccttac ggattgcttt
     5881 tcgagagatt ccttaatccg gcgaggatcc aaatgccgga tatcgacatg gatttcgccg
     5941 atacgagaag ggaagaagtc atcggctatg ttaaagaaaa atacggggac gatcacgtgg
     6001 cccagataat cactttcggg actttggcgg cccgggccgc catcagggat tcgggccggg
     6061 cgcttggcta tccttattct ttcgtcgacc atatcgccaa actgattcct ttcgaaccga
     6121 accagtcgag gtcgagccac cagctcgaag atctgcttga atcgaacgcc gaattccgga
     6181 aagaatacga agccgatccg gacgccaaaa aaataatcga cgccgcttcg aagctcgagg
     6241 gcgtggccag gcacgcctcg gtccacgccg ccggagtcgt catcaccgat aagccgatca
     6301 ccgaatacgc gcctctccag cgatcgccgc aggacgaaac cgcctatatc acccagttcg
     6361 agatgcacgc catcgaagac ctgggcctcc ttaaaatgga ctttttgggg ctccgaaacc
     6421 tgaccatcat cgagggctgc ctcaggcttg tcaaagaaaa aggagattcg atcgacatca
     6481 ataagctccc tctgaacgac caagacactt tcaacctgct ccagcgcggc gaaaacatcg
     6541 gcgttttcca atttgaaggc gcgggaatga cgcgctggct catggccatg aaagccgacc
     6601 gtttcgaaga cctggtggcc atggtcgcgc tgttccgtcc gggaccgatg gagcttattc
     6661 cttcgtatat cgcgagaaag cagggtcgcg aaccgataac ctatctccat ccgaagctcg
     6721 agccgatttt gagcggtacc tacggtatca tgatctacca ggagcaactc ctcatgatcg
     6781 cccgcgaaat ggcgggattc acctacggcg aagccgatgt tttgagaaaa gccgtcggca
     6841 aaaagatcaa atccttgctc gacgagcagg ccggcaaatt cgtttccggc gtggagaaaa
     6901 ccctgggaga caaaaagctc ggagaagacc tctggaaact ggccgagcct ttcgccaggt
     6961 acggattcaa caaagcccat tcggtctgct atgccctgat cggatacgaa accgcttact
     7021 tgaaagccca ttatccgcag gaattcatga cgagcctttt aaataacgat tcttcggacg
     7081 tggagcgtat cgccgttctc gtcgccgatt gccgccgcat gaaaatccag gttctgccgc
     7141 cggatgtgaa caagagctat tcggatttcg ctccggagaa ccagaatatc cgtttcggat
     7201 tgtcggccgt caaaaacgtc ggtgtcggca tcacccaggc cattgtcgac gaacggctcc
     7261 gcggcggacc tttttccaac cttgaagatt ttgtttcgag gatcaaccac aaagatctga
     7321 acaagaaatc tttggaaagt ctcatcaaat gcggcgccat ggattctttg agcgtcgaga
     7381 gaaaaacact tttaatgaac ctcgacgaca tcattagaac cttgagcgca tatcgtcgga
     7441 gtttggccgt gagccagacc agtcttttcg gaggagcgcc gaagatgacg atcaagctga
     7501 cgccggtcgc cccggcgacg aatatggaaa aacttaattg ggaaaaagag cttttgggat
     7561 tgttcgtttc ggaccacccc ttgaacggat tcaagcacaa caacgacggc gtcactccct
     7621 taaaggatgt caaaaacatc cgcgacggat acaatgtgac ggtgacgggt cttgtttcca
     7681 aaatccaaaa aatcaacacc aaaaacggac agccgatgct gtttgtaaaa ctggaagatt
     7741 tgagtaataa tgtggaaata ttggtcttca acgacaccat ccaaaagcat cccgacattt
     7801 ggagagaaaa cacgatactg caagtaaaag gacgagtatc cagaaaaaac ggagaaccaa
     7861 aaataatatg cgaatcagca aaaataatat aaagaaaccg gccggctcga aaaaagccgc
     7921 cggaaaaccg gatctcgagg cgatcagaca ctcgatttcc cacatcgtcg ccttggcgac
     7981 cctcgaaaga tttcccaagg ccaaactcgg catcggtccc acgatcgaaa acggctttta
     8041 ttacgacttc ggaatcaagg agatttccga agacaacttg aaagccatcg aaggccgcgt
     8101 aagagagctt ctgaaacagg gactgaaatt caaaaaagag atcgttactc cgaccgaagc
     8161 gagaaaaatc ttcaaagacc agccgttcaa gctggaactg gtggatgaac tccggaaagc
     8221 gaaaaaaccc ttgagcgtct acaaaaccta cgacaagagt ttgaaaaatc ctctttttat
     8281 cgatctttgc gccggacctc acgtcaaatc gactgcggag atcgatcccg aagctttcaa
     8341 gctcacttcg atcgccggag cttactggag aggaaacgaa aaaaatccca tgctccaacg
     8401 catctatgga ttggccttct caaacaagaa agagctcgat gattatctcc ggctgctgga
     8461 agaggcggaa aaacgcgatc acaaaaagct cggcaaagaa ctcggaatat tcttcattga
     8521 cgagacggct ccggggatgc cttactggct tcccaaagga acgatcatgg tgaacgagct
     8581 cctgaagttc tggcgcgagg agcacaaaaa aagaggctat ctcgaaacca tcactccttt
     8641 gctcaataaa aagcagctgt acgagacttc ggggcattgg gaccattatc gggaaaatat
     8701 gttcataagc gacatgggcg aggacggcgt ttactgtctg aaaccgatga attgcccgaa
     8761 cgcgatgact attttcaagc aggagactca tagctatcgg gaattgccgt tgcgtctttc
     8821 cgattgcgac acccttcacc gccacgagaa gtccggagaa ctgaacggcc tttttcgcgt
     8881 ccaaaaattc gcccaagacg acgcccatat ctttgtcacc gaagagcaaa tcagcgacga
     8941 attcaagaga atcctggaaa tcgccaatct tttctattcg atcttcgaca tcaaattcaa
     9001 gatcagactc ggaacaaaac cggacgatta tatgggcgac gacaaaactt gggcgcgcgc
     9061 cgaaaaagaa ttggaggcca tcttgaagaa aaacaagttc gattatttca tcggtcaggg
     9121 cgaaggcgct ttctacggtc cgaagctcga tatcttgatg caggattctc tgggacggca
     9181 atggcaaacc ggaacaatcc agctggattt ccaactgccc agaaaattcg acctcaaata
     9241 caccgataaa gacggaaaag aaaagactcc ggcggtcatc caccgggtaa tctacggctc
     9301 gctcgaaaga tttatgggta ttctcataga gcactt
//
LOCUS       DAOPQT010000004         8578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_9212_length_8578_cov_2.360202, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000004 DAOPQT010000000
VERSION     DAOPQT010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9212_length_8578_cov_2.360202"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..626)
                     /locus_tag="PKG74_00205"
     CDS             complement(222..626)
                     /locus_tag="PKG74_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89742.1"
                     /translation="MRKPLILTCILLIIAAVFYFAYQKNLEPIIILIYPNGGETIKKG
                     SIETIKWKTKGIPEDYKIALSIRRITDDQIEGQEFDPIIFTDLPNTGNAEWPVSDSYP
                     DGDYILGINVYASTPIINPIYDESDSVFRITE"
     gene            1140..1583
                     /locus_tag="PKG74_00210"
     CDS             1140..1583
                     /locus_tag="PKG74_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89743.1"
                     /translation="MIIGWVEAYYKSSETVKNVDNNEYNFEEFTSFRGKFSPKISLGT
                     TGGFGFSSGFYNRYNLKDSVALKLFYDKSKMAIGFKFLKNTEDGAMKLKPRETGGYVA
                     ARSFLSKYGIEPGRYAGRYDPKEIMDDNIGKIYVIELEEKKNSQT"
     gene            1727..2083
                     /locus_tag="PKG74_00215"
     CDS             1727..2083
                     /locus_tag="PKG74_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89744.1"
                     /translation="MPNTGLSGPYWLSWEVINKVITRTSPGTYVLEREDSTDSFIVNY
                     VGRSDDDVNNRLKKWVGVNGYKRFKLGYFDSPKAAFEKECAIYHDFSGLDNEIHPQRP
                     AGTSWRCPCCNTFNSL"
     gene            2209..3204
                     /locus_tag="PKG74_00220"
     CDS             2209..3204
                     /locus_tag="PKG74_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HOB89745.1"
                     /translation="MENKSLNKNLHKANRAKNDEFYTQLPDIENELKHYKDQFRGKVV
                     YCNCDDPFESNFFKYFAANFKELGLKKLIATSFSGSPITGKQLSILEMEGLKNEKKKE
                     PLKIEITEVLDSDKDGAINIDDVKELLKRCKKTVTPLKGGGDFRSEECIGLLKQADII
                     CTNPPFSLFREYVAQLVEYKKKFLIIGNDNAVKYKEIFPLIVENRIWRGYTRIKEFRR
                     PNGSLQKFGNVGWFTNLDMAKRHEKITLYKKYIRKEYPEYDNYDAVEVSKISEIPMDY
                     DGVMGVPITFIDKYNPDQFEIIGISNGNNRDKYAIGKMVTVNNKSTYARILIKKR"
     gene            3214..3552
                     /locus_tag="PKG74_00225"
     CDS             3214..3552
                     /locus_tag="PKG74_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89746.1"
                     /translation="MEFLKNLKLNNWWSLLVYIGFFGIIGSLFIKTDFVDSKNIFGVS
                     LGFLFIGLSTLSAIKTAIKFKEANAYTGGPAILCWPIIKHNFFSGLITLIGATLAVIF
                     GTRTIVSLFW"
     gene            3559..4650
                     /locus_tag="PKG74_00230"
     CDS             3559..4650
                     /locus_tag="PKG74_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOB89747.1"
                     /translation="MEIKLHQIPIREVIAGYKDSQEEGVTAYNDKLDIRPKYQREFVY
                     KEKQRNAVIETVKQDFPLNVMYWMIREDGGYEVLDGQQRTISIGQYVNGDFSLNERYF
                     HNLTKEEQDQILDYKLMIYFCEGTDKERLDWFKIINTVGEQLTEQEIRNAVYTGPWLS
                     DAKLKFSKSNCVAYLLANDGGKLLEGSPIRQEYLETALSWINNGKIEDYMARHQHDKN
                     ANELWEYFQNVIEWTRKTFPNYRREMSGVNWGDLYNEFKDKKFDTKKLEKEIAELMED
                     EDVTKKSGVYPYILTRKEKYLSIRTFTDKQKREAYERQNGICPICSKHFGIEEMEADH
                     ITPWSKGGKTISENCRMLCQKDNREKSNI"
     gene            complement(4739..5101)
                     /locus_tag="PKG74_00235"
     CDS             complement(4739..5101)
                     /locus_tag="PKG74_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89748.1"
                     /translation="MEEFNKKLLCANCKSVKKTKFCDNCQQETPNFIKLEIRAIIKTQ
                     TSLGIKQKRPGFKRFVKEIFSGYKSSGDKKLSKGVSIEQTIDRENNKYDQVVKDNQTG
                     EIIHEEHEPLSDHNKHKS"
     gene            complement(5109..5678)
                     /locus_tag="PKG74_00240"
     CDS             complement(5109..5678)
                     /locus_tag="PKG74_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89749.1"
                     /translation="MPIPTPTSQIVKNYLAKWDRSEYFVIQDKALRLLFEKTYPKNTK
                     IEHILIKVDFLNQAYSTNLYYPFMVARHILKLNIDEDLRRGDYRIIDKIAKVKTPRGD
                     KKFYSFASKYCSFHYPERYPIYDSFIEKVLWHFRRRDDFYEFRRKDIKEYEKFVEILN
                     KFKEFYGLTEFNAKDLDRYLWQIGKEFFF"
     gene            complement(5737..5810)
                     /locus_tag="PKG74_00245"
     tRNA            complement(5737..5810)
                     /locus_tag="PKG74_00245"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5776..5778),aa:Gln,seq:ctg)
     gene            6048..6284
                     /locus_tag="PKG74_00250"
     CDS             6048..6284
                     /locus_tag="PKG74_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89750.1"
                     /translation="MIIASKIEIKNSDGSWSPYPSVTWLDGVESDDQLQTRAVILSPS
                     GKGFDVVEGLQGVPDGTLMSAAEKAGVHTEFIDG"
     gene            6391..6975
                     /locus_tag="PKG74_00255"
     CDS             6391..6975
                     /locus_tag="PKG74_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89751.1"
                     /translation="MADEVYRLSGFFIPFRYLRKALLCWLDIRASFSDVARKEHWLDW
                     LKRKLHQWFMVQKVLEMLDAEGKCDPFAVSADFDLGITLEECDNVVVFIAPHCCERII
                     LEVASKLDLILTVDSFSKTLEKGTSLMQLYENADCVLGTVVDRSLGTCTCQRLAGFDG
                     EPWIFRIEGDRVYLSGSPIAKTALQNICLGLFSL"
     gene            complement(7054..7911)
                     /gene="fmt"
                     /locus_tag="PKG74_00260"
     CDS             complement(7054..7911)
                     /gene="fmt"
                     /locus_tag="PKG74_00260"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOB89752.1"
                     /translation="MNNFVFFGTPEFAAIILKNLVKAGFVPKAVVANLDKPIGRKRVI
                     TPPPAKQIVLDSGKNIKILQPEKITPEFLETLKSIPADFYLVAAYAKILPQELIDIPR
                     LGVVGVHPSLLPELRGASPIQSAILEGKTRTGVSLFLIDALMDHGPVLRQKTIPIDPD
                     DNSETLTEKLAELSSKMLVEILPDIIDGKITPEIQDETKATFTEKFKTESAFIEEKDL
                     ISAKSGNTELAKKIHNKIRAFYPEPGAWTILNGKRVKLLKSKINETGKLKLEIIQIEG
                     EKPKATDIF"
     gene            complement(7934..>8578)
                     /locus_tag="PKG74_00265"
     CDS             complement(7934..>8578)
                     /locus_tag="PKG74_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89753.1"
                     /translation="GEDIVNPGRTTEYGMQCELDYECCTYTDIYGEPYDCDPNGQNCK
                     RDVIGQDRDCYDRSASRARKTQIRVVGKPEVTFTALPTEILYSSEDIMIRKYSTLNWD
                     SSAPYLGSATAISCNMGIVSGDASSRTGNNPLSSRSSERVAPQRTTTYSLICRNRDDA
                     PNPDPSCFLDSDIKETIVRVFGTGIREEGPPEGSYLEKLDKFMGSIVNIFKKSE"
BASE COUNT         2565 a         1537 c         1915 g         2561 t
ORIGIN      
        1 ttttccatgc cttgagaaaa gcttcttgag aaatgtcggc cgcgtcgtct ttgttcccgg
       61 taagccggaa agcgaaattg tagaccgcat tcaaatgttc gcgaacaagc tcggcgaacg
      121 aagaagcgga agcgtattca gctgttttgt tgtttttaga cacaacaact aataataagc
      181 cacgattgga gataaaaagc aattgtcgga gaaaaccgaa cttattccgt tatcctgaaa
      241 accgagtcgc tttcatcgta aatcggattg ataataggcg ttgaagcgta aacgtttatt
      301 cccaaaatat aatcgccatc ggggtagcta tcggagaccg gccattcggc gtttcctgtg
      361 tttggaagat ccgtgaaaat aatcgggtcg aattcctgac cctctatttg atcatcggtt
      421 atccgccgga tgctcaaagc aattttataa tcttcgggaa taccctttgt tttccacttg
      481 atggtctcta ttgagccttt tttgatcgtt tctccgccgt tcggataaat aagtataatt
      541 attggctcaa gattcttttg gtatgcaaaa taaaagacag cggcaattat aagtaatata
      601 caggtaagaa ttaaaggttt tctcatggtt ttattatagc agtttattaa aatcttgtat
      661 tattaaggga atatgattca aattcctctt agtcttactt gggaaattat taaaggagtc
      721 ggttatattt ttgttaaatt atggtacgtt tggttagtcg tgataggaat taacttgtta
      781 tattggtggt ttgataagcg ggtagctaag aagaaaaagg aatcaaaaaa cagagataac
      841 ctttttaatt attattaact cattctgcgc tatttcaata aaaagtactg agcttgtcga
      901 agtgttgcta tgtccgatct agagccataa aagatgagat tattaaagaa tttctatttt
      961 tagactttat ttcatcactt cgatggactt aaggtttaca aataattgat attaagatag
     1021 gttcatcata ctcaactgat cggcgttgga caataggaca aaacaggaca gaaggcaaag
     1081 atatgttagg gagcatgctt taatcaaggg tcttgacaag aagacgaaga ggacctaata
     1141 tgataatagg ctgggtcgaa gcctactata agtcatcgga aacagtcaag aacgtggaca
     1201 ataacgagta caactttgag gagtttacct ctttcagagg taagttttct cctaaaataa
     1261 gtcttggaac taccggcggc tttggtttca gttcaggctt ttataatcgc tataacctta
     1321 aagactctgt agccctaaaa ctgttttatg ataagagtaa aatggctata ggatttaagt
     1381 tcctaaaaaa cacagaagat ggagcgatga agttgaaacc aagagagacg ggtgggtatg
     1441 tagctgctcg ttcatttctt agcaaatatg ggatagagcc gggaagatac gcgggcaggt
     1501 atgatccaaa agaaataatg gatgataata tcggaaagat atatgtcatt gaattagaag
     1561 aaaagaagaa tagtcaaact tgaatacaaa acgccaagct cagaaaactt ggcgccttgt
     1621 agattataaa tctgtgggat tgggaattcg ccggtcaaag gtcgtcccaa ttaccacaac
     1681 ctttcggcta ttttattata gctaaaagtt taataatagc aatactatgc cgaatacagg
     1741 attaagtgga ccttattggc tttcttggga agtaattaat aaggtcatca ctagaacttc
     1801 acctggaact tatgttttgg agcgtgaaga ctctactgac tcgtttatcg ttaattatgt
     1861 cggtcgttct gatgacgatg tgaataatcg tttaaagaag tgggttgggg taaatggcta
     1921 taagcgtttc aaactcggtt atttcgattc accgaaggcc gcgtttgaga aagaatgcgc
     1981 tatttaccac gactttagtg gattagacaa tgaaatccac ccacagcggc cagctggaac
     2041 gagttggcga tgcccgtgct gcaatacatt taattctttg tgataagcgt gtagaaaaaa
     2101 gatgctacca ttaacggtag catcttttta ggttttatta gttcatctca aatcttgaaa
     2161 aactaaccag aaaaggggat aatagggtgg atttgtcaat agtgaaatat ggaaaataaa
     2221 tccttaaata agaatctgca taaggcaaac cgcgccaaga atgacgagtt ctatactcaa
     2281 ctgccggata ttgaaaacga actcaagcat tacaaagatc aatttcgcgg taaagtagtt
     2341 tattgcaact gcgatgatcc gtttgaaagc aactttttta agtatttcgc ggccaacttc
     2401 aaagaacttg gactaaaaaa gcttattgct accagttttt ccggttcgcc gattaccggt
     2461 aaacaactat caattcttga aatggagggc cttaaaaacg agaaaaagaa ggagccgtta
     2521 aaaatcgaaa taaccgaagt gttggatagc gataaagacg gcgcgataaa cattgatgat
     2581 gtaaaggaat tattaaaacg ctgcaagaag accgttacgc cccttaaagg cggtggagat
     2641 tttcgtagtg aagaatgtat aggacttctt aaacaagccg atattatttg tactaatccg
     2701 cctttttctc tttttcgtga atatgtcgcg caacttgtag aatacaagaa aaagttcctt
     2761 attatcggca atgacaacgc tgtaaaatat aaagaaatat ttcctcttat cgtggaaaat
     2821 agaatttggc gcggttatac aagaatcaaa gagtttagaa gacctaacgg tagcttacaa
     2881 aagtttggta atgtcggttg gttcacgaat cttgatatgg caaaacgtca cgaaaaaatc
     2941 actctttaca aaaaatatat aagaaaagaa tatccagaat atgataatta tgatgcggtc
     3001 gaggtatcaa agatttctga gattccaatg gattatgatg gcgtgatggg cgttccgatt
     3061 acatttatag ataaatataa cccagatcag tttgaaatta ttggtatatc aaatgggaat
     3121 aatcgtgata agtatgcaat aggtaaaatg gtgaccgtaa ataataagtc aacctacgca
     3181 cgaattttaa ttaaaaagag ataatttttt aatatggagt ttctaaaaaa cttaaaatta
     3241 aataattggt ggagtctatt agtatacatt ggattctttg gaataattgg ctcgcttttt
     3301 attaaaaccg attttgtaga ctctaaaaat atttttggag tttctctggg ctttttattt
     3361 atcggattat caacgttatc tgcgataaaa actgctatta aatttaaaga agcgaacgca
     3421 tatactggcg gaccagcaat actttgttgg ccgataataa aacacaattt cttttcggga
     3481 ttaataactt taatcggagc gacattagct gttatttttg gaacaagaac aattgttagt
     3541 ttattttggt aaaacaatat ggaaattaaa ttacatcaaa ttcctatacg cgaggtaata
     3601 gccggttata aggatagtca agaagaaggc gtgacagcct ataacgataa attagatatc
     3661 cggccgaaat accagcgtga gtttgtatat aaagaaaagc aacgcaatgc ggtgatagaa
     3721 acagtcaaac aagactttcc actgaacgtg atgtattgga tgatccgtga agacggcggc
     3781 tatgaagttc tggacggcca gcagcgtact attagtatcg gccaatatgt taacggtgac
     3841 ttttcactaa acgaaaggta tttccataac ttaacaaaag aagagcaaga tcagattctg
     3901 gattacaagt taatgattta tttctgcgag ggtacggata aagagaggct ggactggttt
     3961 aaaatcatta ataccgtcgg tgaacaattg actgagcagg aaatcaggaa tgcggtttat
     4021 accggaccgt ggctctcgga cgctaaatta aagttctcca aatcaaattg tgtggcgtat
     4081 ttacttgcaa acgatggcgg aaaactactt gagggttcgc cgattcgaca ggaatattta
     4141 gaaaccgcac tttcttggat taataacgga aaaatcgaag attatatggc tcgtcatcag
     4201 catgataaaa atgccaacga attgtgggag tatttccaaa acgtgattga atggacgcga
     4261 aaaacttttc cgaattaccg tcgcgaaatg agcggagtta attggggtga tttatacaac
     4321 gaatttaagg ataagaaatt tgacaccaaa aaattagaaa aagaaatagc cgagcttatg
     4381 gaagatgaag atgttactaa aaagtccggt gtttatccgt atattttaac gaggaaagaa
     4441 aaatatcttt caattcgcac ttttaccgat aaacaaaaaa gggaagcata tgaaagacaa
     4501 aatggtatct gtccgatatg cagtaaacat ttcggaatcg aagaaatgga agccgatcat
     4561 atcacgcctt ggagtaaagg tggaaaaaca atctcagaga actgccgaat gctctgccag
     4621 aaagacaata gagaaaagag taatatatag tttttaaatt atccagtcta aaatcatcta
     4681 tctagactgt tgaagggcta ttcggggtcg gttttgaaat ctggctggct cgtctcggtt
     4741 aggatttatg tttattatga tcagatagtg gctcgtgttc ttcgtgtatt atttctcctg
     4801 tctgattatc tttaactact tggtcgtatt tgttgttttc tcggtcgatt gtttgttcta
     4861 tactaacgcc tttagatagc tttttatctc cactcgactt atatccagag aatatctctt
     4921 taacaaatct tttaaatccg ggtcgttttt gtttaatacc taatgatgtc tgagttttaa
     4981 taattgcacg aatttctaat ttaataaaat tcggtgtttc ttgctgacag ttgtcacaaa
     5041 acttagtttt ttttaccgat ttacaatttg cacagagcag ttttttatta aactcttcca
     5101 ttgtattttt aaaagaaaaa ctcttttccg atttgccata aataacgatc taaatcttta
     5161 gcgttgaatt cggtaagtcc atagaactcc ttaaatttgt ttaatatttc gacaaatttt
     5221 tcatattctt taatgtcttt tcttctaaat tcataaaaat catctcgcct gcggaagtgc
     5281 caaagcactt tttctataaa agaatcatag atcggatagc gttccgggta gtgaaaacta
     5341 caatactttg acgcaaagga ataaaatttc ttatctcctc ttggagtttt aaccttcgca
     5401 atcttatcaa taatcctata atcaccacgt cttagatctt cgtcgatgtt aagctttaaa
     5461 atatgtcttg caaccatgaa cggataatag aggtttgtgc tgtaagcttg attcaaaaaa
     5521 tcaactttta taagaatatg ttctattttt gtattcttgg gataggtctt ttcaaagagg
     5581 agtctcagcg ctttatcttg aataacaaag tattctgaac gatcccattt cgcaagataa
     5641 ttctttacta tttgagatgt gggcgtgggt ataggcacag ctcgattata gcattttaag
     5701 acaacccgcc tcggtcgcga gaaactgcgc tcaaagtggc tgccggacct ggattcgaac
     5761 caagatacca tgctccagag gcatgtgtcc taccattaga cgatccggca atacggatag
     5821 attataggcg gaaaatcgat aaaaataaag aggcttgcct ttacatgaag actgctttat
     5881 aagtagatta ttaggtcctt tttggagggg atacgaagtg agagaaatcc gaacgacgga
     5941 tatcatgagg gggggcgccg caagatccca ttaccgtacg tcttgacgac aaagacgtaa
     6001 tcgagctcga atgccatcac cgttacaata acgcgggccc aagccgggtg atcatcgcaa
     6061 gcaaaatcga gatcaaaaac tccgacgggt cctggagccc ttacccgagc gttacctggt
     6121 tggacggagt agaaagcgat gaccagcttc agacaagagc ggttatcctg tcgccttctg
     6181 gaaagggttt cgatgttgtg gagggtctgc aaggcgttcc ggacggaact ttgatgtcgg
     6241 ccgccgaaaa ggcgggagtt cacaccgaat tcatcgacgg atgataaggt gtagtcaagc
     6301 ctgctcttcg gagtaggctt ttttgacatt tggcttgaaa tatggtctta ttataataag
     6361 atcattaaga attggaggga aagtagtaat atggctgacg aagtttatag gctttccggt
     6421 ttctttatcc cattccggta tctgcggaaa gctctactgt gctggctcga tataagagcc
     6481 agtttcagcg atgtggctcg caaagaacat tggctagact ggctgaagcg taagcttcac
     6541 cagtggttca tggttcagaa agtcttggaa atgctagatg cggaagggaa gtgtgatccc
     6601 ttcgcggtca gtgccgactt cgacctcggg atcactttgg aggaatgcga taatgtagtg
     6661 gtttttatcg ctccccattg ttgcgaaaga atcatcctgg aggttgctag taagctggac
     6721 ctcatcttga cggttgactc tttctccaaa actttggaga aggggacttc tttgatgcag
     6781 ttatacgaga acgccgactg cgttctcggc accgtcgttg atagatctct gggcacttgt
     6841 acttgccaaa ggctcgccgg tttcgatgga gagccgtgga tattcaggat cgaaggtgat
     6901 cgagtctatc tttccggttc tccgatcgct aagacggcgt tgcagaacat ttgtcttggt
     6961 cttttctctt tgtaacataa attgaggctt cgcttttggc ggagcctctt ctgttttata
     7021 accctacttt ttttccactc gatcgcggta gtttcagaag atatccgtcg ctttgggttt
     7081 ttctccttcg atctggatga tttcgagttt caatttgccg gtttcattga ttttcgattt
     7141 caaaagtttg acgcgttttc cgttcaatat cgtccaagct ccgggttccg gataaaaagc
     7201 ccggatttta ttatgaatct ttttcgccaa ctccgtattt ccggatttgg ccgaaattaa
     7261 atccttttct tcgataaaag ccgattctgt tttaaacttt tccgtgaaag tcgctttggt
     7321 ttcgtcttgg atttcggggg tgatttttcc gtcaataatg tccggcaata tttcgacaag
     7381 cattttcgaa gaaagctcgg ccagcttttc ggttaaggtt tcgctgttgt cgtccggatc
     7441 gatcggaatg gttttttgcc ggagaaccgg accgtgatcc atcaaagcgt cgatcaggaa
     7501 aagcgatacc ccggttcggg ttttgccctc tagaatcgcg ctttgaatgg gagaggcgcc
     7561 acgaagctcc ggaagtaagg aggggtgaac gccgacaacg cccaagcggg gaatatcgat
     7621 caattcttgg ggcaggattt tggcgtaagc cgcaacaagg taaaaatccg ccggaatgga
     7681 tttaagtgtt tccaagaact ctggtgtgat cttttctggc tggagaattt taatgttttt
     7741 tccggaatcg aggacgattt gcttggccgg cgggggagtg atcacgcgct tccggccaat
     7801 cggcttgtct agattcgcca ccaccgcttt gggaacaaag ccggctttga cgaggttttt
     7861 taggataatg gccgcgaatt ccggcgtacc gaagaaaacg aaattattca ttaagaaaga
     7921 atcggaaaaa tcgtcattcg gatttcttga agatattgac gatacttccc ataaatttat
     7981 cgagtttttc caaatagctt ccttccggcg gcccttcttc gcgtatgccg gtaccgaaga
     8041 ctctaacaat agtttcttta atgtcggaat cgaggaaaca ggaaggatcg ggattcggag
     8101 catcgtctct attcctgcat atcagggaat aagtagtggt tctttgggga gccactcttt
     8161 ccgagcttcg agaagatagg ggattgtttc cggttctaga agaagcatca ccggagacaa
     8221 tgcccatatt gcaggaaata gcggtagcag agcctagata tggagccgaa gaatcccagt
     8281 taagggtaga atacttcctg atcattatat cttcggaaga atagagaatt tccgtaggaa
     8341 gggcggtaaa ggtgacttcc ggttttccga caactcggat ttgggttttt ctcgcgcggg
     8401 aagcggaacg gtcgtagcag tcgcgatctt gtccgatgac atctcttttg cagttttgtc
     8461 cgttcggatc gcaatcgtaa ggttctccgt aaatatcggt ataagtgcag cattcgtaat
     8521 cgagttcgca ttgcattccg tattcggtgg ttcttccggg attgacgatg tcttctcc
//
LOCUS       DAOPQT010000005         8576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_9215_length_8576_cov_3.596761, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000005 DAOPQT010000000
VERSION     DAOPQT010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9215_length_8576_cov_3.596761"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..735
                     /locus_tag="PKG74_00270"
     CDS             139..735
                     /locus_tag="PKG74_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89754.1"
                     /translation="MHFYLSLAILAFMEGALIFKRSFLKKHAKAIFVFSIAVIFLVGL
                     YYSFDLYSVWKTNPVTAAMLPPTSPINYFIFYVFVWAFAPYVLSLMAALVFFLAFRIS
                     NKKSGEKYFENEEPWFAATAIFLCSWPGWLVYLVCLILIYLLIHLFSKIKAKFLNQTP
                     KSERIPLYYLWVPLAIFVIVACQLWLPAILPFWSKLLL"
     gene            842..2548
                     /locus_tag="PKG74_00275"
     CDS             842..2548
                     /locus_tag="PKG74_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOB89755.1"
                     /translation="MLQIPDETLKSLLLREGLIDAEKFDETAKETKRLGQNMADVLIS
                     QGVITKDYWINILSDYFKVPLASLNAKTVNENILRLLPEDVARQRRVILFGQEPDGTV
                     SAAMEDPSNLETIEFIERYIKAKIRPYLAVDADLNKGFMLYSQRQSEDFRKLIETNIA
                     ESLRLGKRGEEAAADVPIVAIVDNLIAYAFSLRASDIHFEIFEDGILVRYRIDGVLRE
                     ILRISKDVHPAIVARIKLLAALKIDEHYKPQDGRFRHKIGPDILDIRVSIIPTYYGEK
                     VEMRLLPSTQRPLSLQELGMFDDMAELVDDNIKKSYGMLLVCGPTGSGKTTTLYSILG
                     MLNRSEVNIVTIEDPIEYDIKYINQTQINPVAGITFATGLRSILRQDPNIIMVGEIRD
                     EETASISVQSALTGHLVLSSLHTNDAPTVVPRLIDMKVPPFLAAAVLNAIVAQRLVRK
                     ICTNCIYSLSPSSELVESLRRQMKELGLEEQFTPPKVVYAGRGCPVCGGSGYRGRMAI
                     FEVLNITEQIRKIIVDPNFSLDVLKAEARKEGMISMFEDGIRKAERGLTTIEEILRVI
                     RE"
     gene            2553..3767
                     /locus_tag="PKG74_00280"
     CDS             2553..3767
                     /locus_tag="PKG74_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOB89756.1"
                     /translation="MKFRYIASQPSGKVTEGTVEANSPAQVLEWMATQNLRPISLKAV
                     EAKKTGQGLRGLFSPAVTVDDQVFLTKYLSLMLKVGTDLFHAIDILIADFDKPVVKSL
                     LIEIRDNLGQGRPFYSTFAKYPQYFSSVFINLVRAGEASGNLDRVFEDLSVTLEKEKD
                     LRGRIRSAVTYPILLLVFAFVILFALVAFALPKISDSFTQGGAEPPAFSRIVFGFGNF
                     IGDHIAIIIFMIVAGVAGIWFFLAKTLPGKKFGVKLINRLPVIGAVLKQLALQRFAST
                     FASLLKAGLPMIEALEITANAVGSEELKEILFRISRQGVAKGVTVGEAFRREPYFPRV
                     VVNLIAISEKAGHMETILETLADFYASDVDARIKVLVSLLEPALLVMIGAVVAIIALA
                     VIVPIYQLVSSI"
     gene            3775..4251
                     /locus_tag="PKG74_00285"
     CDS             3775..4251
                     /locus_tag="PKG74_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOB89757.1"
                     /translation="MYSYKNKQSGFSLVEIVLVFAMTAIIVTISTSNLFGVRNKYALN
                     TTAEKIVFDLRLARENARGQKDGTAWGVRLNNDSNGKFFEVFSGDSYASGTKSLRTAL
                     SSGVKFVTPAEGTNADIIFAKVTGLPASSYSITIGSESITSITKIITINANGTITY"
     gene            4226..4888
                     /locus_tag="PKG74_00290"
     CDS             4226..4888
                     /locus_tag="PKG74_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89758.1"
                     /translation="MRTARSHINGQSLIEALVALMVGVIMIGGVSTVLILSVRSSADA
                     DQFNSASRLASQELDNVRVFAEADWNAIYLATKYVSPENTNPHYLSVVNVGGKTSFAI
                     NNGTTAITVDSVEYTKSFYIENVQRDEDDNVVASGGTDDPSTQKITVNVTWLDGQSLS
                     VVGYLVRSRNVSALFSSWGAGPGLEGPYTILSDSPQGFSSASDDIDFAPEVGGITLII
                     EQ"
     gene            4885..5898
                     /locus_tag="PKG74_00295"
     CDS             4885..5898
                     /locus_tag="PKG74_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89759.1"
                     /translation="MKIPANKKIVFGCALAVFLTAFLNLHVTEAATNIAPDIPPAYLH
                     WAWSDVVGWIDFYSTDSVNVTTTELQGYARYSAEGDGAAPWQYISLNCASGPSGSSCS
                     PISYKVSNTGSGELSGWAWSDAVGWISFSCENEGTSGCAGRNYGVSVVNSADYPGQAE
                     LQGFAWNDIAGWISFNCSNPHVGNPNGICGTVNYKVVTGWSIEPMEGGVVSSTFDTGE
                     ADGVAYNYLLWKGVTNSGDVRFQFATSNCGNGATNAPSCDQNVGWGGAKTSGDGAFLG
                     PSGTSAPEDLYSGSGPDVPIPLKNRGTHTNKRYFRYRIVLYSDITRTLAPRIDGVIVN
                     WSP"
     gene            5899..6369
                     /locus_tag="PKG74_00300"
     CDS             5899..6369
                     /locus_tag="PKG74_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89760.1"
                     /translation="MNKIYKKNRKGVATLPTVIMIGGIIIELAIAGVVVGYAFTESGS
                     GMRLSTEALFTARAGADDAIYKVIRGGYQPSYTLIVSGTPDTPSERKSEITIEKDPVD
                     LEGRYGTCNVIWGCYFRVRSLGLAANRRRKIESVLSVDPATREIRISYIKEIPV"
     gene            6374..7909
                     /gene="pilM"
                     /locus_tag="PKG74_00305"
     CDS             6374..7909
                     /gene="pilM"
                     /locus_tag="PKG74_00305"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOB89761.1"
                     /translation="MAETIIKSRNKLASFWRLINPFKPVGGLSINDSSLKYVRIDENN
                     RLVKASLRLPPGLIEDGKIKNRDLTKQALETLKSYIAPPRGDRRTNVVVSLQDNLIYS
                     RIFNVPNLEEKALEEAAVLNLQMISPIDLKQSYYSYQSVGITANGVRQLELLGAFIPN
                     NIVDDWLSLLRESGFSPIAVEFQSLSMVRAMDYLKLTDPKSAYVALVVAADGLNFAII
                     KNNNLYFNYFYSWKSVGSGGRQIAFVDLEQAIFTEIERVLNFAAARFGTSISKIFVVA
                     EGLGAKIAASIKTRFPRLDSQEFSLMSSAGLPADWASALGSAIRGALPRSKDQFISLN
                     AVSVREEYFQEQSLSLASAWRSAFLVVLSFLAVLYGGSDLLLRRVKEDLVASQTINFP
                     INENQEYAELKAQADSFNALVNLVASAKVRETVISPFFGRIRELAGNDATILRISTQP
                     VSGTVILNATSPSLDMSRRFVDRLKAAPQLQSVEQPLANLVVDPDGSVTFIVSFKVTS
                     QNF"
     gene            complement(7862..8401)
                     /gene="pth"
                     /locus_tag="PKG74_00310"
     CDS             complement(7862..8401)
                     /gene="pth"
                     /locus_tag="PKG74_00310"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOB89762.1"
                     /translation="MGKQKIVFGLGNPGKEYAKTYHSVGRIFLDFLKKKIDQGNDWQA
                     SKKEGFEFVKTPGMILVKSLGFMNDSGVAAASIVKHSRITAKDLIVVHDDSDISLGSY
                     KFSVDRGSAGHKGVQSIIDCLKTPAFSRIRIGVRPTNQKKQAGDFVLNQISKRDAEIL
                     DKVFLKILTRHFEADNKSD"
BASE COUNT         1988 a         2240 c         2182 g         2166 t
ORIGIN      
        1 tttacgcgcc catttggaaa gcaccaaaca tcggccgctt tcgacgcaag acggagacga
       61 caggaataat tgtgccgact atctcgcaac cacttgctgg tattctgtcg gaaccgatcc
      121 tggtctggat ggagcataat gcatttttat cttagtttag ccattttggc ttttatggag
      181 ggagcgctta ttttcaagcg ctctttcttg aaaaaacacg cgaaagcgat ttttgtgttt
      241 tcgatcgcgg ttatttttct cgtcggatta tattattcct ttgatcttta ttctgtttgg
      301 aagacaaacc cggtgaccgc ggccatgctg cccccgacct cgccgatcaa ttatttcata
      361 ttctatgttt ttgtttgggc tttcgcgcct tatgtcttgt ctttaatggc ggctttggta
      421 ttttttctcg cttttagaat cagcaataaa aaatccggag aaaaatattt cgaaaacgaa
      481 gagccgtggt ttgccgccac cgccattttc ttgtgctctt ggccggggtg gctcgtatat
      541 cttgtttgtt tgattttgat ttatcttttg attcatttgt tttctaaaat caaagctaag
      601 tttttaaatc aaacgccaaa aagcgagcgt attccccttt attatctttg ggtgcctctg
      661 gccatatttg tcatcgtagc gtgtcaatta tggcttccgg caatattgcc tttctggtcg
      721 aaacttttac tctgatttcc cgggcgttcg gcgttgattt tgaatagtcg gaccatcatt
      781 aatttgttat actgataatg agatattatt cttgcgtctt tttaacagac gcgatattca
      841 catgcttcag attcccgacg aaactttaaa aagccttctt ctcagagaag gtttgatcga
      901 cgcggaaaaa ttcgacgaga cggccaaaga aaccaagcgc ctcggccaaa acatggctga
      961 tgttttgatt tcccaaggcg tcattaccaa ggattactgg atcaatatcc tttcggatta
     1021 tttcaaggtg ccgctcgcca gtttgaacgc gaaaaccgtc aacgaaaaca tcttgagact
     1081 gcttcccgaa gacgtggctc ggcagcgccg ggtgattctt ttcgggcaag agcccgacgg
     1141 aaccgtttcc gcggccatgg aagatccgtc gaatttggaa acgatcgaat tcatcgaacg
     1201 ctacatcaaa gccaaaataa ggccgtatct cgcggtcgac gccgacctga acaaaggctt
     1261 catgctctac agtcagcgtc aatcggagga tttccgtaag ctcatcgaaa cgaacatcgc
     1321 cgaatcgctt cgactcggca agcgcggcga agaagcggca gccgacgtgc cgatcgtggc
     1381 cattgtcgac aacctgatcg cttacgcttt ttctctccgg gcttcggata tccatttcga
     1441 gattttcgag gatggcattc ttgtccgtta ccgcatcgac ggcgttctgc gggagatcct
     1501 ccggatttcc aaagacgtcc atccggcgat cgtcgcccgc atcaagctcc tggcggctct
     1561 taagatcgac gagcactaca aaccccaaga cggaaggttc cgccacaaaa tcggtcccga
     1621 catcctggat atccgtgttt ccatcatccc cacctattac ggagaaaaag tggaaatgag
     1681 gcttttgcct tcgacccagc ggccgctttc cctccaggag ctcggcatgt tcgacgacat
     1741 ggccgaactg gtggacgaca acatcaagaa aagctacggc atgctccttg tctgcgggcc
     1801 gaccggctcc ggaaaaacaa ccacgctcta ttcgatcctc ggaatgttga accgttcgga
     1861 ggtgaatatc gttaccatcg aagacccgat cgaatacgac atcaaataca tcaaccagac
     1921 gcagatcaat ccggtggcgg gcatcacttt cgcgaccggt ctccggtcca ttctccgtca
     1981 ggatccgaac atcatcatgg tcggcgagat ccgtgacgaa gaaaccgcct ccatttccgt
     2041 ccagtcggct ttgaccggac acttggtttt gtcgtcgctt cacacgaatg acgcgccgac
     2101 ggtcgtcccg aggctgatcg acatgaaagt gccgccgttt ttggcggcag cagtcttgaa
     2161 cgccatcgtc gcccaaaggc tggttcggaa aatctgcacc aactgcattt attccctttc
     2221 tccttcttcg gagctcgtcg aatcgctccg tcgacaaatg aaagaacttg gactcgaaga
     2281 gcaattcact cctccgaaag tggtttacgc cggacgcggc tgtccggtct gcggcggatc
     2341 gggttatcgg ggccgtatgg ccattttcga ggttctgaac atcaccgaac aaatccgcaa
     2401 gatcatcgtc gatccgaatt tttccctcga cgtcttgaag gccgaagccc gcaaagaagg
     2461 aatgatctcc atgttcgaag acggaatccg gaaagccgaa agaggcctca ccactatcga
     2521 ggaaatattg agagtcatca gggaataaaa aaatgaaatt ccgctacata gcttcccagc
     2581 cgagcggcaa agtgaccgaa ggaacggtcg aggccaattc tccggcccag gttttggaat
     2641 ggatggccac gcaaaatctc cggccgattt ccttgaaggc ggtggaagcg aaaaaaaccg
     2701 gtcagggcct gagggggctt tttagtccgg cggttaccgt cgacgaccag gtatttttga
     2761 ccaaatacct gagtctgatg ctcaaagtgg gaacggattt gttccacgcg atcgatattt
     2821 tgatcgccga cttcgataag ccggttgtca aatcattact gatcgagatc agggacaatc
     2881 tcgggcaagg aaggcctttt tattcgactt tcgccaaata tccccagtat ttttcctcgg
     2941 ttttcatcaa cttggtgagg gctggagaag cttccggaaa cctcgatcgg gtcttcgaag
     3001 atttgagcgt cactctggaa aaagaaaagg atttgcgggg ccgcatcagg tcggccgtca
     3061 cctatccgat cctgcttttg gttttcgcct ttgtcattct tttcgccttg gtcgctttcg
     3121 ctcttccgaa aatctccgat tcctttaccc agggaggagc cgagccgccg gctttctcga
     3181 gaatcgtctt tggtttcggg aactttatcg gcgaccatat cgccatcatc attttcatga
     3241 tcgtcgccgg cgttgccgga atctggttct tcctcgcgaa gactctgccc ggaaagaaat
     3301 tcggggtcaa gcttataaat cgtctgccgg tcatcggcgc cgtattgaaa caattggctt
     3361 tacagcggtt cgcttcgact ttcgcttcgc ttttgaaagc cgggcttccg atgatcgaag
     3421 ctttggaaat cacggcgaac gccgtcggat ccgaagagct gaaagagata cttttcagga
     3481 tttcccggca gggcgtggcc aaaggcgtga ctgtcggcga agctttccgg cgcgaacctt
     3541 attttccgag ggtggtggtc aatctgatcg ccatttccga aaaagccggg cacatggaaa
     3601 cgatccttga aaccctggcc gatttttacg cttcggatgt cgatgcgaga atcaaggtgt
     3661 tggtttcctt actcgagccg gcgcttctcg ttatgatcgg agcggtcgtg gcgatcatcg
     3721 ccctcgcggt catcgttccg atttatcaat tggtatcctc gatctaacga acacatgtat
     3781 tcttataaaa acaaacaatc gggtttttct ctcgtcgaaa tcgtgctcgt cttcgccatg
     3841 acggccatca tcgtgacgat ctccacgagt aatcttttcg gggtgaggaa caaatacgcc
     3901 ctgaacacca ccgccgaaaa aatagttttc gatttgcgtt tggcccggga gaacgcccgg
     3961 gggcaaaaag acgggaccgc ctggggcgtc agactgaaca acgattccaa cgggaagttt
     4021 ttcgaagttt tttccggtga cagttacgcg agcgggacaa aatccttacg gacggctctt
     4081 tcttcgggag tgaaattcgt gactccggcg gaaggaacga acgccgatat tattttcgcg
     4141 aaagttaccg gtttgcccgc ctcttcttat tccattacaa ttggatcaga gagtatcact
     4201 tcgatcacca aaataatcac catcaatgcg aacggcacga tcacatatta acggccaatc
     4261 gcttattgag gcgctggtgg ctctgatggt cggcgttatc atgatcggcg gcgtatccac
     4321 cgtcctcatt ttgtcggtgc gttcgagcgc cgacgcggat caattcaaca gcgccagccg
     4381 tttggcgagc caagaactcg ataacgtcag ggttttcgcc gaagccgatt ggaacgccat
     4441 ttatctcgcg accaaatacg tcagtccgga aaacaccaat ccgcattatc tttccgtggt
     4501 gaacgtcggt ggcaaaacca gtttcgccat caataacggc acgacggcga taaccgtcga
     4561 cagcgtcgaa tatacgaaat ccttctatat agagaatgtc caacgcgatg aagacgacaa
     4621 tgtcgtcgcg agcgggggaa cggacgatcc ttcgacgcag aaaatcaccg tcaacgtcac
     4681 ctggctcgac ggccaatcct tgagcgtggt cggctatctt gtccggtcgc gcaatgtttc
     4741 ggctcttttc tcaagctggg gagccggccc cggactcgag ggaccgtata ccattctttc
     4801 cgactctccc cagggatttt cttcggcttc cgacgatatc gatttcgctc cggaggtcgg
     4861 aggaataacg ctcatcatag aacaatgaaa attccggcaa acaaaaaaat cgttttcgga
     4921 tgcgccttgg ccgtattctt gaccgcgttt ttgaatttac acgtgaccga agccgcgacc
     4981 aatatcgcgc cggatattcc gccggcttat cttcattggg cctggagcga tgttgtcggc
     5041 tggatcgatt tttattcgac ggacagcgtg aacgtgacca cgaccgagct tcagggatac
     5101 gcgagataca gcgccgaagg cgacggagcg gctccctggc aatacatctc tctgaattgc
     5161 gcgagcggac cctcgggctc gagctgcagc cccataagct acaaggtctc gaataccgga
     5221 tccggagagt tgtccggctg ggcctggagc gacgccgtcg gctggatcag cttcagctgc
     5281 gaaaacgaag gcacgagcgg ctgcgccgga cgcaactacg gagtatccgt cgtgaacagc
     5341 gccgattatc cgggccaggc ggagctgcag ggttttgcct ggaacgatat cgccggctgg
     5401 atcagtttca actgcagcaa tcctcatgtt ggtaatccga acggcatctg cggcactgtt
     5461 aactacaaag tggttaccgg atggagcatc gaaccgatgg agggaggagt ggtctcgagc
     5521 actttcgata ccggcgaagc cgacggtgtg gcttacaatt atctcttgtg gaaaggcgtg
     5581 accaattccg gagacgtcag attccaattc gccacctcca attgcggcaa cggcgcgacc
     5641 aatgctccga gctgcgacca aaatgtcggc tggggcggcg ctaaaacttc cggcgacggc
     5701 gctttcttgg gaccgtccgg aacttcggct ccggaagatc tttattccgg ttctggtccg
     5761 gatgtgccca tacccctcaa aaacagggga actcatacca acaaaagata tttccgttac
     5821 cggatagttt tatacagcga cataacccgg acgcttgctc cgcgaatcga cggagtcatc
     5881 gtcaattgga gtccataaat gaacaagatt tataaaaaaa atcgtaaagg agtggctact
     5941 ctgccgacgg tcatcatgat cggcggtatt atcatagagc tcgccatcgc cggagttgtt
     6001 gtcggctacg cctttaccga gagcggatcg ggtatgagac tttcgaccga ggccttgttt
     6061 accgcgcgcg ccggcgccga cgacgccatc tataaagtca tcagaggagg ttatcagcct
     6121 tcgtacactt tgatcgtttc cggaacgccc gacaccccga gcgaacgcaa atccgaaatc
     6181 accatcgaaa aagatccggt tgatctcgaa ggccgttacg gcacctgcaa cgttatctgg
     6241 ggatgctatt tccgggtgcg gtcgcttgga ctcgccgcta accgaagaag aaaaatagag
     6301 tccgtgcttt cggtcgatcc ggcgacgcgg gaaatcagaa tcagctatat caaagaaata
     6361 ccggtttaaa atcatggcgg agacaatcat caaatcgcgg aacaaattgg cgtctttctg
     6421 gagattgatc aacccgttca agccggtcgg cggtttgagc atcaacgatt cttctttgaa
     6481 atacgtccgc atcgacgaga acaatcgttt ggtcaaggct tctttgagac tgccgccggg
     6541 attgatcgaa gacggaaaga tcaaaaaccg ggatttgacg aaacaggcgc tcgagacttt
     6601 gaaatcttac atcgctcctc cgcgcggcga tcgtcggaca aacgtcgtcg tctctctcca
     6661 ggacaatttg atttattcga ggattttcaa cgttcccaac ttggaggaaa aagctttgga
     6721 agaggcggct gttctgaatc ttcaaatgat ttccccgata gatttgaagc agtcttatta
     6781 cagctaccag tctgtcggga tcaccgcgaa cggcgtccgc cagctggagc tcctcggagc
     6841 ttttattccg aataacatcg tcgacgattg gctttctctt ttaagggaat cgggattcag
     6901 cccgatcgcc gtcgagttcc agagtttgag catggtgagg gcgatggatt atttgaaact
     6961 gaccgacccg aaatccgctt acgtcgcctt ggttgtcgcc gccgacgggt tgaacttcgc
     7021 catcatcaag aacaacaatc tttatttcaa ttatttttac tcctggaaat ccgtgggctc
     7081 cggaggccgg cagatcgcct ttgtcgattt ggagcaggcc attttcacgg aaatagagcg
     7141 cgttttgaac ttcgccgccg ccaggttcgg cacgtccatt tccaagattt tcgtcgtggc
     7201 cgaaggcctc ggcgccaaaa tcgccgcttc gatcaaaaca cggtttcccc ggctcgacag
     7261 ccaggaattt tctttgatgt cttccgccgg attgccggcg gactgggcat cggctttggg
     7321 gtcggccatc aggggtgctt tgccccgctc gaaagaccag ttcatcagct tgaacgccgt
     7381 gagcgtcagg gaagaatatt tccaggagca gtctttgagc ctggcctcgg cctggcgaag
     7441 cgcttttctc gttgtcctct cgtttctggc ggtgctctac ggcggaagcg atttgctttt
     7501 gcgccgggtg aaggaagacc ttgtcgcgag ccagacgatc aattttccga tcaacgagaa
     7561 tcaagaatac gccgaactca aagcccaggc cgacagcttc aacgctttgg tcaatttggt
     7621 ggcgagcgcc aaagttcggg aaacggtcat ctctccgttt ttcgggagga ttagggaatt
     7681 ggccggcaat gacgccacta ttttgagaat ctccacccag ccggtcagcg gcacggttat
     7741 tttgaacgcc acttctccgt cactcgatat gtcgaggaga tttgtcgata gactgaaagc
     7801 cgctccccag cttcaaagcg tcgaacagcc gcttgccaat ctggtggttg atccggacgg
     7861 atcagtcact tttattgtca gcttcaaagt gacgagtcag aatttttagg aagactttgt
     7921 cgagaatttc cgcatcgcgc ttggaaatct gatttaaaac aaagtctccg gcttgttttt
     7981 tctggttcgt gggccttacg ccgatgcgga tcctcgagaa agcgggcgtt ttcaggcaat
     8041 cgatgatcga ttgcacgcct ttgtgcccgg ccgaacccct gtcgacggaa aatttgtaag
     8101 aaccgaggct gatatcggaa tcgtcgtgaa cgacgatcaa atccttcgcc gttatccggg
     8161 aatgtttgac gatcgaggcg gccgcgactc ccgagtcgtt catgaatccg agggatttca
     8221 cgagaatcat ccccggagtt tttacgaatt cgaatccttc ttttttcgag gcttgccaat
     8281 cgtttccttg atctattttc tttttcagga aatccaaaaa aatccttccc acgctgtgat
     8341 aagtcttcgc gtattctttc cccggattac ccaatccgaa aacgatcttt tgcttgccca
     8401 tcagcgtcaa ttgtattaaa atgcgacgga tgaagcaatt attagccgtt tgggagttcc
     8461 tcgaagtggc ggcgatcgcc attatcatcg cctgcgcttt caagtttttg gcccagccct
     8521 ttttggtcca gggcgccagc atggagccta acttcgacga cggagatttt cttctc
//
LOCUS       DAOPQT010000006         8067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_9979_length_8067_cov_3.417249, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000006 DAOPQT010000000
VERSION     DAOPQT010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9979_length_8067_cov_3.417249"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKG74_00315"
     CDS             complement(<1..291)
                     /locus_tag="PKG74_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89763.1"
                     /translation="MKFVLRSIVFWLVVGSIMVCMVLLKNNGWKTYADKIVSLWKAGE
                     PGSCLILEEKYCRSGKFIYPFEDKTMAIGFKLPEETLVYAPYDGLTGFLIPGP"
     gene            complement(291..767)
                     /locus_tag="PKG74_00320"
     CDS             complement(291..767)
                     /locus_tag="PKG74_00320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012195892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOB89764.1"
                     /translation="MTKKQYIIIASVVLLAIILILQNKRSFLSKPGENTSEETGELIS
                     SVPLSSPDDQLKFDIIKVGEGPEIKNGDLATVNYVGQLKDGTIFDSSYERGVPFQFLL
                     GAGHVIRGWDMGVLGMKVGEKRELIIPARHGYGDQGIGSIPGGATLIFEVELLKIN"
     gene            complement(785..1768)
                     /locus_tag="PKG74_00325"
     CDS             complement(785..1768)
                     /locus_tag="PKG74_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOB89765.1"
                     /translation="MNNNFWQNIKKPFFCSAPMYGVSDEAFRRMLLKYGRPDIFFTEM
                     VSADGLVYMAEKTGMDSKQAGVFLETLRFVPEERPIVAQVFGSRPENFYKSVLLLRQM
                     GFGGIDINAGCPDKNIEKQGAGASLIKNPGLVKEIIAAAKEGAAGSGLSLKTRIGHDA
                     KETEDWIGFLLEQGLDALIIHGRTRSQGFGGKADWLMIKKTVELRDKASFRTIIVGNG
                     GLTDMKDAKNKLEESGVDGAMIGRALIGNPWFFSDQAKEREGAEKTKAILEHLAIFEE
                     NFKIKNFDDVKKHLAAYASGFLGAKELRMELMKAKSVEEARKVVAEFGSKS"
     gene            complement(2051..3709)
                     /gene="argS"
                     /locus_tag="PKG74_00330"
     CDS             complement(2051..3709)
                     /gene="argS"
                     /locus_tag="PKG74_00330"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOB89766.1"
                     /translation="MLEKIANLLSEAVKEDEVKVFIPENPDFGHYSTNLAFEFAKMRE
                     ISPDEAGKEIRNKLTHAAPKGFFSRVDIAGGFLNFTLSDETLEKEINLILRQKQNYGR
                     TRKGHKKTVVVDYSAPNIAKPMNVGHLRSTIIGQAIVNLLRFEGYRVIGDNHLGDWGT
                     QFGGLLAAFKKWGSREELKRDPIDYLVKLYIRFNKEAETNEELMNEARMETAKLQKGD
                     RENRRLWKEFVRASLKEFNKTYRRLGVRFSVVLGESFYQKMLPAVVKDALEKGVAKKD
                     DGAIKIALGDEKMPDIVIEKTDGSHLYATTDLAAIVYRVRKWHPEKILYVVANEQALH
                     FSQIFKAAEILKIAPQTELEHVKFGMLLGESGQKMSTRRGEFIKLEELLDRAKEEAGK
                     IDQKSAEQVGIGAVKYFDLSHDRRSDIVFDWNKVLNLKGNSAPYILYTYARLRSVLRK
                     AGPKIKKRLDLSLLRTEAEKKTVRQLVYFPDMVAAAAENYMPNYLADYLYKLANDLNA
                     FYETSPVISVEKNLAESRLALVAAGSVVLKQGLKLLGIETLEQM"
     gene            3728..4171
                     /gene="smpB"
                     /locus_tag="PKG74_00335"
     CDS             3728..4171
                     /gene="smpB"
                     /locus_tag="PKG74_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOB89767.1"
                     /translation="MAVYVQNRSANFEYDILEKFEAGLALLGSEVKSLRSGKASISGA
                     YATVKGNEAFLIGAEISPYQPKNTPKNYDPGRTRQLLLTREEIKELTGKIRKTGLTLV
                     PIRIYSKGRTVKLELGLGRKKKKFDKREAIKRRETSREIERSLKK"
     gene            4192..4540
                     /gene="ssrA"
                     /locus_tag="PKG74_00340"
     tmRNA           4192..4540
                     /gene="ssrA"
                     /locus_tag="PKG74_00340"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4586..5395)
                     /locus_tag="PKG74_00345"
     CDS             complement(4586..5395)
                     /locus_tag="PKG74_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89768.1"
                     /translation="MITNELKKQAGFAPLLIIGVVAAIAIGGTVVIASILKNNSNSQL
                     ILADEQDQINEQNIGLATNYEELKMASKSTSESENTGENKAIITLKDETHEICVEALK
                     QNDPTEKSLLTQIHNICNRLLSGEYALENTDIQEDHLHEIWSLWEKTKEEEAKVVKHQ
                     NEKDDRKSEETEKIIYNTTECLTQKNSLLSQVEAVYLKWYEEWQDARKTLAPCYENNP
                     IPICDKQMTNLNVEWQDKIIVAINEYHSQLLKCAPSEREFSSISNVIPSPY"
     gene            6280..6768
                     /gene="infC"
                     /locus_tag="PKG74_00350"
     CDS             6280..6768
                     /gene="infC"
                     /locus_tag="PKG74_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOB89769.1"
                     /translation="MNNQISAPELRVVDETGKNLGVFPLAKALEMAKAEGMDLIETVP
                     GANPPVVKIMSFDKFRYQRAKELKKQRQSESLTKGLKQIRIGAKTAKNDLLTKIKKIE
                     EFLDDGYKVEIFLRLRGREKGNPDWARKNLEGMLSLITAEHKITSPIRYSGNAFTVQI
                     SK"
     gene            6770..6943
                     /locus_tag="PKG74_00355"
     CDS             6770..6943
                     /locus_tag="PKG74_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOB89770.1"
                     /translation="MRKTVGKRIKVTKTGKLIRRKMSQSHFRAKKSGKAIRAKRPAVK
                     VSKVDIKTFKKYL"
     gene            6947..7288
                     /gene="rplT"
                     /locus_tag="PKG74_00360"
     CDS             6947..7288
                     /gene="rplT"
                     /locus_tag="PKG74_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOB89771.1"
                     /translation="MRVKRGVVAHKKREARLKATKGFKWGRKSKERAAKEALLHAGQH
                     AFQGRKQKKRDYRALWNTKINAGLRENGTKYSVFISKLRKSEIKLDRKTLADLAENHP
                     EAWKKLVESVK"
     gene            complement(7317..7784)
                     /locus_tag="PKG74_00365"
     CDS             complement(7317..7784)
                     /locus_tag="PKG74_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOB89772.1"
                     /translation="MKREISAGLIVFRKTDQGPKFLILYHGGRYWNFPKGHIEAEEKS
                     LETAIRETVEETGIPRTKLYVKKGFKINERYRFFKGKEPIFKIVIFYLAETKDENIKI
                     SSEHEGYGWFLYRDARYILSPYKDSQELLRKAYAFIRPRRRNTDKNSPKSGNS"
     gene            complement(7856..>8067)
                     /locus_tag="PKG74_00370"
     CDS             complement(7856..>8067)
                     /locus_tag="PKG74_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HOB89773.1"
                     /translation="NRLDLKDEYIRLAVNMGVKLSVDSDSHSVRHLRFLDLGLAQARR
                     GWAKKSDIINAWPIEKTLGFLKKNN"
BASE COUNT         2112 a         1995 c         1728 g         2232 t
ORIGIN      
        1 aggtcccgga atcaaaaatc cggttaagcc atcataaggg gcgtaaacca atgtttcttc
       61 gggcaatttg aatccaatcg ccatggtctt gtcttcgaaa ggataaataa atttgccgct
      121 acgacaatat ttctcctcga gaatcaagca actccccggt tcaccggcct tccaaagcga
      181 cacgatctta tccgcatatg ttttccagcc gttgtttttc aaaagaacca tacagaccat
      241 aatcgatccg acgacgagcc agaaaacaat ggatcttaaa acgaatttca ttagttaatt
      301 ttcaggagct cgacttcgaa aatcaaagtc gcgcctccgg gaatgctccc gatgccctga
      361 tcgccgtagc cgtgcctcgc cggaataatc aactctcttt tctctccgac tttcattccg
      421 agcactccca tgtcccagcc ccggatcacg tggcccgcgc cgagaagaaa ctggaaaggc
      481 actccccgct cgtaactcga atcgaaaatc gttccgtctt tcagctggcc gacgtaattg
      541 accgtcgcca gatctccatt tttgatttcc ggtccttctc cgactttgat gatgtcgaat
      601 ttcaattgat cgtccggaga agaaagcgga accgaagaaa tcaattcgcc agtttcttcg
      661 gaagtgtttt ccccgggttt cgacaaaaac gacctcttgt tttgcaaaat aaggataatc
      721 gccaaaagaa cgacactcgc gatgataata tattgcttct tggtcatgac gatttttatc
      781 tattttaact tttcgagccg aattcggcaa ccacttttct cgcttcttcg acggatttcg
      841 ctttcatcaa ttccattctc aactctttcg cccccaaaaa acccgaagca taagccgcca
      901 gatgcttttt gacgtcgtcg aaattcttga tcttgaaatt ctcttcgaaa atcgcgaggt
      961 gctcgagaat ggctttggtt ttttccgcac cttcgcgctc ttttgcttga tccgagaaaa
     1021 accaaggatt gccgatcaag gcccggccga tcatcgctcc gtcaacgccg gattcttcga
     1081 gtttgttttt cgcgtctttc atgtccgtta gtccgccgtt tccgacaatg atcgtccgaa
     1141 aagacgcttt gtcccgaagc tcgaccgttt ttttgatcat caaccaatcc gcttttcctc
     1201 cgaacccctg gctccgcgtt cgtccgtgaa taataagcgc gtccaaacct tgttccaaga
     1261 gaaatccgat ccagtcctcg gtttcttttg catcgtgtcc gattctggtt ttcaaggaca
     1321 atccagaacc ggccgcgcct tctttcgcgg ccgcgatgat ttctttaacc aatccggggt
     1381 ttttgatcag actggcgccg gctccttgct tttcgatgtt tttgtccggg cagccggcgt
     1441 ttatgtcgat tccgccgaat cccatctgcc gaagcagaag aacgcttttg taaaagtttt
     1501 ccggacggct cccgaaaacc tgagccacta tcggacgctc ttcgggaacg aatcgcaggg
     1561 tttccaaaaa cactccggct tgtttcgaat ccataccggt cttttccgcc atataaacca
     1621 aaccgtcggc cgaaaccatc tccgtgaaaa agatatccgg ccgaccgtat ttgagaagca
     1681 tccgacgaaa agcttcgtcg gaaacgccgt acatcggcgc cgagcagaaa aacggttttt
     1741 tgatgttttg ccaaaaatta ttattcattg gccgcaaact ataccttgac ttagttccgg
     1801 aaaagtttca agattgaccg acttttaaca aaaggaggtc ctttccgatg aggtcttttc
     1861 taacgaacct attatctcta gggtatcgca ccgatccctt ggagaaaaga tccgcacgcc
     1921 ttaacatgcg gcctcattgc tccacctcgc gaaagctctt gaataaagac gagcggaagt
     1981 ttatcccgcc gcgctacgga cgcggcagat aaaccagaaa ccccggccaa ttggacgggg
     2041 tttttcatat ctacatttgt tccaaagttt ctattccgag aagctttaga ccttgtttca
     2101 ggacaacgct tccggcagcg accaaagcca atcggctttc ggccaaattc ttttcgaccg
     2161 aaatcaccgg agaagtttcg taaaaagcgt tcaagtcgtt cgccaatttg taaagataat
     2221 cggcgagata attcggcatg tagttttccg cggccgcggc caccatatcc ggaaaataaa
     2281 caagctgcct tactgttttt ttctcggcct cggttcgaag caatgataga tccagtcttt
     2341 ttttgatttt cgggccggct tttctcaaaa cgcttcgcaa acgggcgtag gtgtaaagaa
     2401 tgtaaggtgc ggaattccct ttgaggttta agaccttgtt ccagtcgaaa acaatgtcgc
     2461 tccggcggtc gtgggaaaga tcaaagtatt tcaccgcgcc gatgccgacc tgttccgccg
     2521 atttttgatc tatttttccc gcttcttctt tggcccgatc gagcaattcc tcgagcttga
     2581 tgaattctcc ccggcgcgtg gacattttct gtccgctttc tcccaaaagc atgccgaatt
     2641 tcacgtgctc gagttcggtt tggggagcga tcttcaatat ttcggctgcc ttgaatatct
     2701 ggctgaaatg aagcgcctgt tcgttcgcca cgacatataa aatcttttcc ggatgccatt
     2761 ttcggacgcg gtagacaatg gccgcgagat cggtcgtcgc ataaagatgc gaaccgtcgg
     2821 ttttctcgat aacgatatcc ggcattttct cgtctcccag agctattttt atcgctccgt
     2881 cgtctttttt ggccactcct ttttccaggg cgtctttgac caccgcggga agcatctttt
     2941 ggtaaaagct ctcgccgaga acaacgctga acctcactcc gagtcgcctg taggtcttgt
     3001 tgaattcctt gaggctcgct ctcacgaatt ctttccacaa tctccggttt tcccggtcgc
     3061 ctttctggag cttggccgtt tccatccgcg cttcgttcat caattcttcg ttcgtttcgg
     3121 cttccttgtt gaatcttatg taaagcttca ccagataatc gatcggatct cttttaagct
     3181 cctctcgact accccatttc ttgaaagcgg ccagaagccc gccgaattgg gtgccccagt
     3241 ctccgagatg gttgtctccg atcacccgat atccctcgaa tcgcaggaga ttcacgatcg
     3301 cctggccgat gatcgtgctc cgcaaatgtc caacattcat cggtttggcg atattcggag
     3361 ccgaataatc gacaacaacg gttttcttgt gtccttttct cgtccggccg taattctgtt
     3421 tttgtctcaa gatcagattg atttcttttt cgagcgtctc gtccgaaagc gtgaaattca
     3481 aaaaacctcc ggcaatatcc acccggctga aaaatccttt tggcgcggcg tgggtcagtt
     3541 tgtttctgat ttccttaccc gcctcgtccg gagagatctc gcgcattttg gcgaactcga
     3601 aagcgagatt ggtggaataa tgtccgaaat cgggattttc cggaataaaa accttgacct
     3661 cgtcttcttt taccgcttcg gacaacaaat tagcgatttt ttcaagcatt atctatgatt
     3721 atagcgtatg gccgtttatg tccaaaaccg aagcgccaat ttcgaatacg acatcctcga
     3781 aaagttcgag gctggtttgg cgcttttggg gtcagaagtg aaatccctgc gttcgggcaa
     3841 ggcgagtatt agcggagcct acgccacagt caaaggaaac gaagctttcc ttatcggcgc
     3901 ggaaatcagc ccctaccagc ccaaaaacac ccctaaaaac tacgatcctg gccgaacccg
     3961 acaattgctt ttgaccaggg aagaaatcaa ggaattgacc ggcaaaatcc gcaaaacagg
     4021 cttgacgctc gtgcccataa gaatatatag taagggccgc accgtgaagc tcgagctcgg
     4081 cttgggacgg aagaaaaaga aattcgacaa acgcgaagcc atcaaacgcc gcgagacctc
     4141 gagagaaatc gaacgtagct taaaaaaata gatccttcgg ggttttcata cggggatgcc
     4201 aggccttgac ggaccgatga atggaaaacg gcaagcaaag cttggaagaa gcttttgaat
     4261 cctcttccaa attgataagt gccaacttat tcagacaacc ggctttagcc tacgcttaag
     4321 ccggcatctc gccgccgatt cccaataggc gaatcgcgag gtgttatata ttgggatgcg
     4381 ttccgtggac gcttataagc ggaacaaagg caataagcta aagaattcgt ctttttcgcc
     4441 ggatctaaag gacggattct tctaaaataa tccggctaag cttgtagaag tttttcacga
     4501 ttctctccgg acgcgagttc gattctcgcc atctccacaa aaatcaatta ccaagtttcc
     4561 aaactatatt cagattccgc ggcacttaat atggagacgg aatcacgtta gatatactac
     4621 tgaattctct ttctgaagga gcgcacttta atagttgact gtgatattca ttgatagcta
     4681 cgataatttt atcttgccat tctacattta aattagtcat ctgtttgtca caaattggga
     4741 tagggttatt ttcgtaacac ggggccaatg ttttcctggc atcttgccat tcttcgtacc
     4801 atttaagata tactgcttcg acttgagaaa gaagactgtt cttctgggta aggcattccg
     4861 ttgtattata aataattttt tctgtttctt ctgattttcg gtcatctttt tcattctggt
     4921 gtttaactac tttcgcttcc tcctctttgg ttttttccca taacgaccat atctcatgta
     4981 aatggtcttc ttgaatgtct gtattttcca acgcgtattc accagataaa agtcgattgc
     5041 agatattatg gatttgcgtg agtagagatt tttctgtcgg gtcgttttgt tttaaagcct
     5101 caacgcagat ttcatgggtt tcatctttta atgtaattat tgccttgttt tcaccagtgt
     5161 tttcactttc cgacgtgctc ttagaagcca tttttaattc ctcgtaattc gtcgcaaggc
     5221 ctatattctg ttcgtttatt tggtcttgct catcagctaa tataagctga ctgttgctat
     5281 tattcttgag tatagacgcg ataactactg tgccgccgat agcgattgcg gcgacgactc
     5341 caattatcaa caaaggggca aatcctgcct gtttttttaa ctcattagtg atcattttaa
     5401 tcctctctta tccatctcct ataattctcc tcatttaata atcccttttc ttgtaactca
     5461 taacatttta tccgttcgtt cttctcaacg gctgaatcaa tggcgttcgc gagaatatcg
     5521 atgaaccaga ttaccgcaat aataacaaca atcgtaataa ttattttccc tgttattttt
     5581 agatatttca tttttttata agtcattgtt gtatggttct aatccgtgac ccctcataca
     5641 tgattcgtag taaaaatcat acgttttttc tcgtgctaca taattccttt ctttaagagc
     5701 ttcatgaata gcccgtgaat cacagttttg ccttgagatg ctaggaaaaa caataaatcc
     5761 atagaatgaa aaagataaaa tcagaagcac tactaatata aagactacca atccaacata
     5821 agcaagaatt ctcttctttt tagaggaaac atctttgatt cttttgaata tattcatata
     5881 gttttctatc tatgttaccg gcagtggaga tataaaaatc ccaccaccag gtaggccttt
     5941 cggccgtata cgggtttccc cgcacaacgc atcgagcgat cctgatgatg ggatcttcgt
     6001 gctcgatgcg atttaggacc atgccgccga ttaatcagac ggtttaggcc tgggctaagc
     6061 cctattcagt tgttactcca atattaccct tttaggcacc aaaaatccac gtctctggta
     6121 taattgttac atttaagaat gaagcctccc atcaaaaacc gggatttttt gttggcgatt
     6181 cgtcagcctc tcccggtttg acgccaggac cccgaaatag tatattaaaa aagcgttaaa
     6241 aggcgtttta tccgaaacac gaccataact aatcccaaga taaacaacca aatatccgct
     6301 ccggaactaa gggttgtcga cgaaaccgga aaaaatcttg gagtttttcc attggccaaa
     6361 gctttggaaa tggcgaaagc cgaaggaatg gaccttatcg aaaccgttcc cggggccaat
     6421 ccgccggtcg tgaaaatcat gagcttcgac aagttccgct accagcgggc caaagagctc
     6481 aaaaaacagc ggcaaagcga aagcctaacc aaagggctga aacagataag gatcggcgcc
     6541 aagaccgcca aaaacgacct tctgaccaaa atcaaaaaaa tagaagagtt tttggacgac
     6601 ggctacaaag tggaaatatt cctgaggctg cggggaaggg agaaaggcaa ccccgactgg
     6661 gcgagaaaga atctcgaagg aatgctttct ttgatcaccg ccgaacacaa gatcacgagc
     6721 cccataagat acagcggcaa cgccttcacc gtccaaatat cgaaataaca tgagaaagac
     6781 tgtcggaaaa agaatcaaag tcaccaaaac cggaaaactg atccgccgca aaatgagtca
     6841 gtcgcatttc cgcgccaaaa aaagcggcaa agccatcaga gcgaaaagac cggccgtgaa
     6901 agtgagcaag gttgatatca aaacattcaa gaaatattta taaaccatga gagtgaaacg
     6961 gggcgttgtc gcccacaaaa aaagagaagc ccgattaaaa gccaccaaag gattcaagtg
     7021 gggcaggaag tccaaagaaa gagcggccaa agaggctttg ctccacgcgg gccagcacgc
     7081 tttccagggc cgcaaacaga aaaaaaggga ttaccgggcc ctttggaaca cgaagatcaa
     7141 cgccggactc cgggaaaacg gaacaaaata cagcgttttt atttccaagc tgagaaaatc
     7201 cgagatcaag ctcgaccgga aaaccttggc cgatttggcc gagaaccatc cggaagcctg
     7261 gaaaaaactc gtcgaatccg taaagtaaaa aagtcccgcg tgaagcggga ttttttctac
     7321 gaatttccgg atttcgggga attcttgtcc gtattccgtc tccgggggcg gataaaagca
     7381 taagcttttc tcaaaagctc ttggctgtct ttgtacggag aaaggatata gcgggcgtcg
     7441 cggtacaaaa accagccgta tccttcgtgc tccgaagaaa ttttgatgtt ttcgtctttg
     7501 gtttcggcca aataaaaaat gactattttg aaaatcggct ccttgccctt gaaaaagcgg
     7561 tagcgctcgt tgatcttgaa tccctttttg acgtaaagct tggtccgcgg aatgccggtc
     7621 tcctcgaccg tttccctgat ggccgtttcc agactctttt cttcggcctc gatatggcct
     7681 ttggggaaat tccaatatcg tccgccgtgg taaagtatca aaaactttgg gccttggtcg
     7741 gtcttacgaa aaactatcaa tccggcggat atttctcgtt tcatagatga gcttccgatc
     7801 ttcaaaagaa acggaagaat gattcagtat ccaccaaaaa ccggtttttg gcaaatcagt
     7861 tgtttttctt caaaaaaccg agggtttttt cgatcggcca agcgtttatg atgtcgcttt
     7921 tctttgccca gccgcggcgg gcctgggcca atccgagatc gagaaacctg agatggcgga
     7981 cgctgtgact gtcggaatcg acagatagct tcacgcccat gttgacggcg agtctaatgt
     8041 attcgtcttt caaatcgagt ctgttcg
//
LOCUS       DAOPQT010000007         6897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_12130_length_6897_cov_3.388483, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000007 DAOPQT010000000
VERSION     DAOPQT010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12130_length_6897_cov_3.388483"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PKG74_00375"
     CDS             <1..490
                     /locus_tag="PKG74_00375"
                     /inference="COORDINATES: protein motif:HMM:NF017013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HOB89774.1"
                     /translation="ILLVSNNVALEAMAKQAEELGFRPEIMNDVMDGEAAEAGKKICE
                     TLREEPSQTVLLYGGETVVVVKGDGKGGRNQELALSALRFVKDDELILTLASDGRDNT
                     EAAGAICDIISKTRAAEKGLDPEEYLERNDSFNFFERTRDQIITGPTGSNVADLVIAI
                     KQ"
     gene            498..2921
                     /gene="ppsA"
                     /locus_tag="PKG74_00380"
     CDS             498..2921
                     /gene="ppsA"
                     /locus_tag="PKG74_00380"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOB89775.1"
                     /translation="MKNKPFILWYDKITMKDVPAVGGKNASLGEMIKYLRPKGVNIPY
                     GFATTSEAYHYFIKETGLENLIGETLKNINTKDIKDLARRGKTIRDAFLTKEIPEDLK
                     KEIVVAYRKLGKHLKENNPSVAVRSSATAEDAPTASFAGQMETYLNVRGEEELLKAVL
                     LSMQSLFTDRAIFYREQMKFDHLKIALSSGVQQMVRSDLASAGVMFSIDTETGFPDVI
                     LINASWGLGENVVKGRITPDQYYVFETTLEKGFKPIVGKVLGKKESKLVYGSGKEATK
                     EVATSIADREKFVLTDEEILTLAKWSNLIEKHYGRPMDSEWAKDGVSGKLYMVQARPE
                     TVESRKDVGMLEEYELDRSKKPEELVVGLSVGAKIGQGKAHVIKSVKEITSFKEGEVL
                     ITRMTDPDWVPAMKLAAAIVTDYGGRTAHAAIVSRELGIPCIVGTGNATSIIKSGEPV
                     TVDCSGGEVGRVYKGLVPFKIKKTDVSRIRKPKTKIMMNVGEPGQAFGLSFIPNDGVG
                     LCREEFIIASDIGIHPMALIDYETLKRRNDPKIKTIVKKIDEKTIAWKDKPKFYVDNL
                     AFGIGKIASAFYPKEVIVRFSDFKTNEYRSLIGGELYEPEEENPMIGWRGASRYYHPG
                     FEKAFILEIKAIKKVREEMGLTNVNVMVPFCRTAEEGKKVMKILEKNNLRGRKFLKNS
                     GPKIYVMCEVPSNVILADEFLEVFDGMSIGSNDLTQLTVGIDRDAGELLRGVADENDA
                     SVKASVAKVIERCRALGKYIGICGQAPSDFPDFALFLVKQGIDSISLNPDSVLKTMEK
                     INDFERKRK"
     gene            2896..4065
                     /locus_tag="PKG74_00385"
     CDS             2896..4065
                     /locus_tag="PKG74_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOB89776.1"
                     /translation="MILSEKESRIADIKTEIIKDSRGEDTIKVEVVLDSGASGVCSVP
                     QGKSKGKHEAVYVPAAQAVENIEKTILPDLRDFGFNFQEELDEKLINLDKTENKSRLG
                     ANAILGVSIASGQARAKDLEMEFWDYVRENYGDAPKSTPKILANLVNGGLHAENNLDF
                     QEYMIIPRMDNFAESLKIIREVYSGLEDHFGPKAKKGDEGGFAPDFENNIEPFRIIKD
                     TIEELGYEQRVDFGIDAAASDVRMPVTELFGFYDKLKKDFDIAYIEDPFSEEDFENFR
                     KLETLIGGNTMIVGDDLTTTNVKRMTLAYEKQAINAMIVKPNQIGSLTETLKAIRLCR
                     DYGWGVIISHRSGETMDDFIADIAWAVGAEGIKIGSPSQPERMAKYERLLAIQNP"
     gene            4166..5374
                     /locus_tag="PKG74_00390"
     CDS             4166..5374
                     /locus_tag="PKG74_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOB89777.1"
                     /translation="MRPETLEDFVGQDEIIGVGKMLRKAIEVDEIPSMIFWGPPGCGK
                     TTLARIIAKMTKARFVQFSAASGSVKEIREIVAGAKELLRNKNQKTILFVDEIHRFNK
                     AQQDAFLPHVESGVIILIGATTENPSFEVNSALLSRSRVFVLKALEPEDIEVILKKAL
                     SDKERGFGNLKINISEDSIKFLASVADGDARTALNALELAVKSVKPAIGKKGLKPIKI
                     DDVSIREALQKTHLLYDKKGEEHYNLISALHKSMRGSDPDATLYWLGRMLEAGEDPLF
                     VARRLIRFASEDIGLANPNAMLLAVAAYEAAHFIGMPECNLSLAEAAVYMAKSKKSNE
                     LYLAYGKVQRDIEEMPNEGVPLHLRNAPTKLMKDIGYGKGYKYNPKYKEPVEQDYLPE
                     RLKKRKYLND"
     gene            5376..6086
                     /locus_tag="PKG74_00395"
     CDS             5376..6086
                     /locus_tag="PKG74_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOB89778.1"
                     /translation="MPKLIFDIETVGEDYDALDETTKESLTRWLKKESFGEEEYEAAL
                     EDLKAGLGFSPLTGEIVAIGMLDYDQNKGAVYYQAPGENIPDEEENNVKFKAMTEAEM
                     LKAFWEAASKYDEFVSFNGRAFDAPFLAVRSAINKIRPTKDLMSNRYVSSQKFGAVHI
                     DLLDQLTFYGAVRKKGNLHLWSRAFGIESPKAQGITGDDVGRLFKEKKFLDIAKYNVG
                     DLKATRELYKYWVEYIRM"
     gene            6086..6583
                     /locus_tag="PKG74_00400"
     CDS             6086..6583
                     /locus_tag="PKG74_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOB89779.1"
                     /translation="MLKVRPFQESSNGGYCGPASLKMVLDYYGTKKSESDLAKLCRAY
                     KTNGVEAEDIKRAAESLAFKAVIKNNASFSDIEKYLDKGIPVIVDWFTRGRSDYPDSE
                     VADGHYSVVVGLDKKHIYLQDPEIGKIRKIKRDDFLKVWFDFKGDYMKSPKDLILRQI
                     IAIKK"
BASE COUNT         1991 a         1585 c         1815 g         1506 t
ORIGIN      
        1 catccttttg gtttccaata acgttgcctt ggaagccatg gccaagcagg ccgaagaatt
       61 gggattccgg ccggagataa tgaacgacgt catggacggc gaagcagccg aagccggaaa
      121 gaagatctgc gaaacgctcc gcgaagagcc atcgcaaaca gtacttcttt acggaggaga
      181 gaccgtggtc gtggtcaaag gcgacggcaa aggcggccgc aaccaggaac tggctttgtc
      241 cgccttgcgt tttgtgaaag acgacgaatt gattttgact ttggcttcgg acggccggga
      301 caacaccgag gccgccggcg ccatctgtga tataatttcc aaaacccgcg ccgcggaaaa
      361 agggctcgac cccgaagaat acctcgagag gaacgactct ttcaattttt tcgagcggac
      421 gagggaccaa ataatcaccg gtccgaccgg atccaacgtg gccgatctcg tgattgccat
      481 caaacaataa aaaaacgatg aaaaacaaac cttttattct ctggtacgac aaaatcacca
      541 tgaaggacgt tcccgcggtc ggcggaaaaa atgcttcttt gggagagatg atcaaatatt
      601 tgcgtcccaa aggcgtgaat attccttacg gattcgccac cacttccgaa gcctatcatt
      661 actttatcaa agaaaccgga ctggagaatc tgatcggaga aactttaaag aacataaaca
      721 ccaaagatat caaagattta gcgcggcggg gaaaaacaat ccgcgacgcg tttttgacca
      781 aagaaattcc ggaagatttg aaaaaagaga ttgtggtcgc ttatcgcaaa ctcggcaaac
      841 acttgaaaga aaataatcct tcggtcgccg tgcgttcgag cgccacagcc gaagacgcgc
      901 cgaccgcgag cttcgccgga caaatggaaa cttatctgaa tgtccgcggc gaagaagagc
      961 tcctgaaggc ggttttgctt tcaatgcagt cattattcac cgaccgggcc attttttacc
     1021 gcgagcagat gaaattcgac cacctgaaga tcgctttgtc ttcgggtgtc cagcaaatgg
     1081 tgcgttcgga tttggcttcg gccggagtga tgttttcgat cgacaccgaa accggatttc
     1141 cggacgtgat cttgatcaat gcgagctggg gtttgggaga aaatgtggtc aaaggccgca
     1201 tcacgccgga tcaatattat gttttcgaga caaccctcga aaaaggattc aaaccgattg
     1261 ttggtaaagt cttgggaaaa aaagaaagca agcttgtcta cggttccggc aaagaagcca
     1321 ccaaagaagt ggccacctcg atcgcggatc gcgaaaaatt cgtcttgacg gacgaggaaa
     1381 ttttaacttt ggccaagtgg tccaatctga tcgaaaaaca ttacggtcga cccatggatt
     1441 ccgagtgggc caaggacggt gtctcgggca aactttacat ggttcaagca agaccggaaa
     1501 cggtcgaaag ccgcaaggat gtcggcatgc tcgaagaata cgaactcgac cggtccaaaa
     1561 aaccggaaga gcttgttgtg ggactcagtg ttggcgccaa gatcggtcaa ggcaaagccc
     1621 atgtcatcaa aagtgttaaa gaaataactt ctttcaagga gggcgaagtt ctgatcacga
     1681 ggatgaccga tccggattgg gtgccggcga tgaagctcgc cgcggccatt gtcacggatt
     1741 acggaggccg gaccgcacac gcggccattg tttcccgcga actcggcatt ccctgtattg
     1801 tcggaaccgg caacgccact tcgatcatca agtccggaga gccggtgacc gtcgattgtt
     1861 ccggaggcga ggtcggccga gtctacaaag gactcgtgcc tttcaaaata aagaaaaccg
     1921 atgtttccag aatccgaaaa ccgaaaacta aaatcatgat gaacgtcgga gaaccggggc
     1981 aggccttcgg gttgagtttt atcccgaacg acggcgtggg cctttgccgc gaagaattca
     2041 tcatcgcttc ggacatcggc atccatccga tggcgctcat cgattacgag actctgaaac
     2101 gccgaaatga tccgaaaata aaaacgatcg tcaaaaaaat cgatgagaaa acaatcgctt
     2161 ggaaggacaa accgaagttt tatgtcgaca atttggcttt cggcatcgga aagatcgctt
     2221 cggcttttta cccgaaagag gtgatcgtca gattttccga tttcaaaaca aacgaatacc
     2281 gttcgcttat cggcggagag ctttacgaac ccgaggagga aaacccgatg atcggttggc
     2341 gcggggcttc ccgttattac catccgggtt tcgaaaaagc ttttattttg gaaatcaaag
     2401 ccatcaaaaa agtccgcgaa gaaatggggc ttacaaacgt gaacgtcatg gtgccttttt
     2461 gccgcacagc agaagaaggg aaaaaggtga tgaagattct cgaaaaaaac aatctccgcg
     2521 gaagaaaatt cctgaagaat tccggcccga aaatctacgt catgtgcgaa gtgccttcga
     2581 acgtgatctt ggccgacgaa tttctcgagg ttttcgacgg aatgagcatc ggcagcaacg
     2641 acctgaccca gcttacggtc ggcatcgacc gcgacgccgg cgaattgctg cggggagtcg
     2701 ccgacgaaaa cgacgcatcg gtcaaagcct cggtcgccaa ggtgatcgaa cgttgccggg
     2761 cactcggcaa atatatcggc atctgcggcc aggcgccgtc ggattttccg gatttcgccc
     2821 ttttcctcgt gaagcagggc atcgacagca tttctttgaa tccggattcg gtcttgaaaa
     2881 ccatggagaa aatcaatgat tttgagcgaa aaagaaagta gaatcgcgga tatcaaaacg
     2941 gaaatcatca aggattcccg cggagaagac acgatcaaag tggaagttgt cttggattcc
     3001 ggcgcttccg gagtttgttc ggttccgcag gggaaaagca agggtaagca cgaagcggtt
     3061 tacgttccgg cggcgcaagc cgtggaaaac atagaaaaga cgatccttcc ggatttgcgg
     3121 gatttcggat tcaatttcca ggaggaactc gacgaaaagc tgatcaatct cgataagacc
     3181 gagaacaaat cgcggctcgg cgcgaacgcg atcttggggg tttcgatcgc gtccggacaa
     3241 gccagggcca aggatttgga aatggaattc tgggactatg tccgcgaaaa ttacggagac
     3301 gctcccaaaa gcaccccgaa aatcctggcc aatctcgtaa acggcggctt gcatgcggaa
     3361 aacaatttgg atttccagga atacatgatt attccgcgaa tggacaattt cgcggagagt
     3421 ttaaaaatca tccgggaagt ttattccgga ctcgaggatc atttcggccc caaagccaaa
     3481 aaaggagacg aaggcgggtt tgcgccggat ttcgagaaca acatcgagcc ttttcggata
     3541 atcaaagata cgatcgaaga actcggctac gaacaaaggg tggatttcgg gatcgacgcg
     3601 gccgcgagcg acgtccggat gccggtaacg gaacttttcg gtttctatga taaactcaaa
     3661 aaagatttcg acatcgctta tatcgaagac ccgttttccg aggaagactt cgaaaatttc
     3721 aggaaattgg aaactttgat cggcggaaac acgatgatcg tcggcgacga tctcacaacc
     3781 acgaacgtca aacgcatgac tttggcctac gagaagcagg ccatcaacgc catgatcgtc
     3841 aaaccgaatc agatcggatc gcttacggaa accctgaaag ccattaggct ttgtagagat
     3901 tacggctggg gagtgattat ttcccaccgg agcggagaaa ccatggatga tttcatcgcc
     3961 gatatcgcct gggccgtcgg agccgaagga atcaagatcg gcagtccgtc ccagccggaa
     4021 aggatggcta aatacgagag attgttggct atccaaaacc cctagcaaaa ggggttttga
     4081 tttgtgatag aatacggctg tggccgattt attcgcggaa aaagaggaga aaatattcaa
     4141 agaaaaagcg cctttggccg accagatgag gccggaaacc ctggaggatt ttgtcggtca
     4201 agacgagatc atcggagtcg gcaaaatgct ccggaaggcg atcgaagtcg acgaaatccc
     4261 gtcgatgatc ttttggggcc cgcccggatg cggcaaaaca accttggcgc ggatcatcgc
     4321 caaaatgacc aaggcgcggt ttgtccagtt ttccgccgcg tcggggagtg tcaaggaaat
     4381 ccgggagatc gtcgccggag caaaagaact cttgcggaac aagaatcaga aaaccattct
     4441 tttcgtggac gaaatccatc ggttcaacaa agcccagcag gacgcttttt tgccgcacgt
     4501 ggaaagcggg gtgatcattc tgatcggcgc gaccaccgag aatccgtctt tcgaggtcaa
     4561 ttccgctttg ctttcgcgaa gccgggtttt cgtgctgaaa gctttggagc ccgaagacat
     4621 cgaagtcatc ttgaaaaaag ccctatccga caaagagcga ggattcggta acctgaaaat
     4681 aaacatttcc gaagattcga taaagttctt ggcttcggtc gcggatggcg acgcccgaac
     4741 cgctttgaac gctttggagc tggcggtcaa atcagttaag cccgcgatcg ggaaaaaagg
     4801 tttgaagccg attaagattg acgacgtctc gattcgcgaa gctttgcaga aaacgcatct
     4861 tttgtacgat aaaaaaggcg aggagcatta caatctgatt tcggctcttc ataagtccat
     4921 gcggggcagc gatcccgacg ccaccttgta ttggctggga aggatgctcg aggcgggaga
     4981 agatccttta ttcgtggcgc gaaggcttat caggttcgcc tccgaggata tcggtttggc
     5041 gaatccgaat gccatgcttt tggcggtcgc cgcttacgaa gcggcccatt tcatcggcat
     5101 gcccgaatgc aatctgtcct tggccgaagc cgcggtttac atggccaaat cgaaaaaaag
     5161 caacgagctt tatctggctt acggaaaagt gcagcgggat atcgaggaaa tgccgaacga
     5221 aggcgtaccc ttgcatttgc gtaacgcgcc gaccaaactc atgaaagaca tcggctatgg
     5281 caaaggatat aaatataatc caaaatataa ggagccggtg gaacaggatt atctgccgga
     5341 aaggttgaag aaaaggaaat atcttaacga ttaacatgcc caaacttatt ttcgacatag
     5401 aaaccgtggg cgaggattat gacgctttgg acgagacgac taaagagtcg ttgacccgct
     5461 ggctgaagaa agaatctttc ggagaggaag aatacgaggc ggcgctcgaa gatctcaaag
     5521 ccgggctcgg gttttctcct ttgaccggag aaatcgtggc tatcggcatg cttgattacg
     5581 atcaaaacaa aggcgcggtc tattaccagg ctccgggaga aaatatcccc gacgaagaag
     5641 aaaacaacgt caaattcaaa gcgatgaccg aagccgaaat gctcaaggct ttttgggaag
     5701 cggcctcaaa atacgacgaa ttcgtgagtt tcaacggacg ggcttttgac gcgccgtttt
     5761 tggcagtcag gtcggccatc aataaaatcc ggccgaccaa agatctgatg tcgaaccgtt
     5821 atgtttcgag ccagaaattc ggggcagtcc acatcgattt gctcgatcaa ctcacttttt
     5881 acggagcggt ccgcaaaaaa gggaatcttc atttgtggag ccgggctttc gggatcgaga
     5941 gtcccaaagc ccaaggcatc actggagacg acgtcggccg gcttttcaag gaaaagaaat
     6001 tcttggatat cgccaagtac aacgtcggtg atctcaaagc gacgagagag ctttacaaat
     6061 attgggtcga atatatcagg atgtgatgct aaaggttaga cctttccaag agtcttcgaa
     6121 cggaggttat tgcggtccgg ccagcctgaa aatggttttg gattattacg gaacaaagaa
     6181 atccgaatca gatctggcca aactttgccg tgcttataaa acaaacggag tcgaggccga
     6241 ggacatcaaa agagcggccg agagtttggc gtttaaagcc gtcattaaaa acaacgccag
     6301 tttttccgac atcgaaaaat atctcgataa aggcattccg gtgatcgtcg attggttcac
     6361 gagaggccgc agcgattatc cggattcgga agtggccgat gggcattatt ccgtagttgt
     6421 cggtttggat aaaaagcata tttacttgca ggatccggaa atcggaaaaa tacgtaaaat
     6481 aaaaagagac gatttcctca aagtttggtt cgatttcaag ggagactaca tgaagtcgcc
     6541 gaaagatttg atcttgaggc agatcatcgc gatcaaaaaa taaaatgttt ttctcaaata
     6601 aaaaaaagat tgtccctcaa ttattggagg tttataaacg gagactgcct atgaagcttc
     6661 aaatatctct aagaagatcc gaagacggcg gttatatcgc gtttgtcgat aatgtgtccg
     6721 gttgcgttac tcaagccgaa acaaagcaag agctctttac gcaattaaac gatgctttat
     6781 atacctactt cgaaataccc aaaaattacc aaccttacat gcctaaatta ttgcgttaat
     6841 cggtttagcg ttataatgct tttatggatg aaatctacgt cataggacac aaaaacc
//
LOCUS       DAOPQT010000008         6691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_12595_length_6691_cov_3.154310, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000008 DAOPQT010000000
VERSION     DAOPQT010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12595_length_6691_cov_3.154310"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PKG74_00405"
     CDS             <1..721
                     /locus_tag="PKG74_00405"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HOB89780.1"
                     /translation="RQQKKFTTALQIFFQQQQKEVMKNLKEEGLPKSRGGKIGEKSLH
                     NWINKILFEKAKQDKLIVEMSGDMYRDNIQAGAEAVARLLGIDASDVLATPFVIDFIK
                     DRSFLMLSVNKTTEDALRATLAEGVGLGEDLGQIRDRISSIYDEAQDFRAETIARTEV
                     GAAQNFGRTAEMTNQRVEKKVWIATFSNTRPAHAAADGQIVRVDEAFSVDGEDLEYPG
                     DPNGSPDNTINCQCSTSPTLG"
     gene            837..1883
                     /locus_tag="PKG74_00410"
     CDS             837..1883
                     /locus_tag="PKG74_00410"
                     /inference="COORDINATES: protein motif:HMM:NF016471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HOB89781.1"
                     /translation="MKKKIKLIQKKIDGEVVKIQARTASISFKALGEDGTFEAYASIF
                     GNVDSYGEVVDQGAFKEWLADYFDNGKGRYPKCVWNHNWDEPIAKTLECREDERGLYV
                     KGKFVMEVQRAKEIYALMKEGVITDFSFGFRVLDDEWDEQTNIRHLKKIAIYEYSPVL
                     VGANREATILGVKADGEPIEGAEEVEEEIDIPAKEKEGDEEPKEEEEKDPKDPAEPKP
                     AAEGEGEGEGAGNAGDGSGAGEGQPEQNDNAGSGGEDEEGKGLKQGRVLSAKNREAIK
                     TAVDGMRSASDALEALLEATEDDDSGKSSDTSEVEGHEGKGSPEKKILKGILRDARKA
                     DKMVESIILRAKQV"
     gene            1909..3042
                     /locus_tag="PKG74_00415"
     CDS             1909..3042
                     /locus_tag="PKG74_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOB89782.1"
                     /translation="MNKKIMIQLEGGKEIDLAEVIAESSVEQLKEMGFPIQDGKVNFT
                     SLIQPAKSEKDLKVEGLKEAANFIKTLVLPERLHKQYGVEPVQLKQITTDSGSFGAAV
                     PTALASAILEKKAKFAIIRSRAFAFELSGNFDLPVEGTGVTGYWISGEVDDSDANLVT
                     ASEPTLVKKSLTDHYLAALVKVSWKLLQTSDYNLVNFIASLAGKKLAETEETAFIAGS
                     GTGQPKGIRSESLTSVAQEGAGLAYQDLVNLYFLLPAQYRQNAVFITSAMGAKAIVGL
                     KDSQDRPIFAPGQPLDELFRKPLLESGDIPENLGTGQDTTEVYFGDPYYYWIKDGQAL
                     SMATQDVIERLQTKVLVYQAVDGKLVLTDAFVKLTGVKPAVES"
     gene            3118..3546
                     /locus_tag="PKG74_00420"
     CDS             3118..3546
                     /locus_tag="PKG74_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89783.1"
                     /translation="MNRITIKFLVDYTPYKTGEIATFFEREANRLIDRGFAMKYVAPG
                     QPADSAAEKLKALSNTSTRSIPAAPETKVIPGPEKTKAPEGPKDPEKVEAPQSPINGG
                     NAQTDEGSGQAPKVASKVEGKAKNKGRGRQSGKNKGKKQA"
     gene            3543..4151
                     /locus_tag="PKG74_00425"
     CDS             3543..4151
                     /locus_tag="PKG74_00425"
                     /inference="COORDINATES: protein motif:HMM:NF016990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HOB89784.1"
                     /translation="MNLTTLAKVKSHLGIRASNTTKDAILNQIITGVSGFVEQITNRK
                     FGIQTYTERIDGDDSDEIFLKQYPVVDLISLEIDDEAIGVDAEEEDETLIVDNEIGSI
                     WRDLGFAEGRKNIKVVYTAGYNPPVDTDESSDGSDEDSGAESFPNSLEGAVIRMVARV
                     YERRTAEGVSSVSPGSFSVQYKDFIDDDIQQIIDSHKKRRVS"
     gene            4155..4481
                     /locus_tag="PKG74_00430"
     CDS             4155..4481
                     /locus_tag="PKG74_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89785.1"
                     /translation="MLNFRSFSIYRLTRDEKSGSYGLDFVETGESVSGFLENASPEFA
                     AIAGGAYGKIFSLFSDDFDVDVKTSDRLIDENDPAIKFDVRGVLIDEDAPGRKLQITL
                     SQAIKQ"
     gene            4483..4974
                     /locus_tag="PKG74_00435"
     CDS             4483..4974
                     /locus_tag="PKG74_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89786.1"
                     /translation="MELKLQLEGDVLKKIKVANEKTRQLFKDAVRDGSTLMMESAIRS
                     TPASTGNLRKSIRREISGSGLRATIFPSVVYGAFLHGEFQGGPAYSKPFTIPAREARE
                     GGTLYRWAKKKGLNPYAVRASIAKKGIKHNPFLKAAAEESTPGVQAIFERALDKLAAS
                     LGD"
     gene            4981..5394
                     /locus_tag="PKG74_00440"
     CDS             4981..5394
                     /locus_tag="PKG74_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89787.1"
                     /translation="MYSIIRQALLDLLNGESITKIKQAYRTEVSEISAYPVAIIAPSE
                     HDADYHQTSPSSNKETYIFTIRVIYPFTEGQATADLALEQALDELIGVLRDKTCLGSA
                     ADWIKPVPGRWGYEQREGGVMRIAELNVQAVKYVG"
     gene            5526..6533
                     /locus_tag="PKG74_00445"
     CDS             5526..6533
                     /locus_tag="PKG74_00445"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HOB89788.1"
                     /translation="MIQRGEEINMGVGLEDARGTGVAPQVWIPGRTPSGVVPVVEKVA
                     LKETRGSKIASHGAEIIKSRAEGDHEFNLRVQSIGYLLKSLLGAVSSQAKNAPNGAVY
                     DHTFSVLPNDPEHPSLTIGIAQSSGQDYKYSLGVVSNLSIEMTPDDLVKAVASFIASA
                     EAEQSPPYVPAFSDDDVYFRHQDITIKMADDMSGLAAAAALKVKSMKIDIPNGGRADH
                     NVSELNPGNVLATQLDPKVAFEVDYQNEDLHDSYTGGEYFALQITMERADITIGSNQH
                     PKLVITFPKCSLSKWTPNRPIDDVVREQVEAMVHYDEDEAKAISVVLTNLLTGYEPAE
                     S"
BASE COUNT         1839 a         1688 c         1800 g         1364 t
ORIGIN      
        1 gagacagcaa aagaaattca cgaccgcgct tcaaatcttt ttccagcaac agcaaaagga
       61 ggtcatgaaa aatctcaaag aagaaggact cccgaaaagc cgcggcggaa agatcggaga
      121 aaaatctctc cacaactgga tcaacaaaat tctctttgaa aaagcaaagc aagataaatt
      181 gatcgtcgag atgtccggcg atatgtatag ggataacatt caggccgggg ccgaagccgt
      241 cgcgagactg ctcggcatcg atgcgtccga tgtattggcg acgccgtttg tgatcgactt
      301 tatcaaggac cggtcgttcc ttatgctctc ggtcaacaag acgaccgagg atgctctccg
      361 ggcaacgctc gccgagggcg tcggacttgg cgaagatctc ggacagatcc gcgatcggat
      421 ctcctcgatc tatgacgagg ctcaagattt ccgggctgaa acgatcgccc ggaccgaagt
      481 cggcgcggcc caaaacttcg gccggaccgc cgagatgact aatcaaaggg tcgagaaaaa
      541 agtttggatc gcgactttct ccaatacccg gccagcgcat gcggccgctg atggacagat
      601 cgtccgcgtc gatgaagcct tctccgttga tggagaagat ctcgaatatc cgggagatcc
      661 gaacggctcg ccggataaca cgatcaattg tcaatgttcg acctcgccga cgcttggata
      721 attgcgcgat tcttgtttct gttttataat taaaaaaaga aaagagatcc cacgggatcg
      781 caaggctcaa gtctatggac cggaagccga gcggtcctct tttcgtaaat atcactatga
      841 agaaaaaaat aaaactcatc caaaagaaaa tcgacggcga agtcgtaaag attcaagcga
      901 ggaccgcgtc gatctcattc aaggctctcg gagaggatgg aactttcgag gcttacgctt
      961 cgatcttcgg gaatgtcgac tcttacggcg aggtcgtcga tcaaggcgcc ttcaaagaat
     1021 ggctcgccga ctatttcgat aacggcaaag gccgatatcc gaaatgcgtt tggaatcaca
     1081 attgggacga gccgatcgcg aaaacccttg aatgccgcga ggatgagcgc ggtttgtatg
     1141 tcaaaggcaa attcgtgatg gaagtccaac gcgcgaaaga gatctatgct ctcatgaagg
     1201 agggcgtgat cacggacttc tcgttcggct tccgcgtttt ggatgatgaa tgggatgagc
     1261 aaacgaacat ccggcacttg aagaagatcg ccatttatga gtattccccg gtccttgtcg
     1321 gcgcaaatcg cgaagcgacg atcctcggcg tgaaagccga cggcgagccg atcgaaggcg
     1381 ccgaggaagt cgaggaggaa atcgatattc cggccaaaga gaaagaagga gacgaggagc
     1441 cgaaggaaga agaagaaaag gatccgaaag atccggccga accaaaaccg gccgcagaag
     1501 gagaaggcga aggagagggc gccggcaacg ccggcgacgg atccggagcc ggagaaggtc
     1561 aacccgagca aaacgacaac gccggctcgg gcggagaaga tgaagaaggg aaaggactga
     1621 aacaagggcg agtcctctcc gcgaagaacc gcgaggccat caaaacggcc gtcgacggca
     1681 tgcggagcgc atcagacgca ctcgaagcct tattggaggc aacagaggac gacgattccg
     1741 gaaaatcgtc ggacacttcc gaggtcgaag ggcatgaagg caagggatct cccgagaaga
     1801 aaattctcaa agggatcctc cgtgatgccc ggaaggcgga taagatggtc gaatcgatca
     1861 tcctccgcgc aaaacaagtt taatcagcat aattgaacca ttctcatcat gaacaaaaaa
     1921 atcatgattc aactcgaggg cggcaaggag atcgatctcg cagaggtcat cgctgaatct
     1981 tccgtcgagc aattgaaaga gatgggcttt cctattcagg acggcaaagt caacttcact
     2041 tctttgatcc aaccggcgaa atccgagaag gatctgaaag tcgaaggctt gaaggaagcg
     2101 gccaatttta tcaaaacttt ggtccttccc gagcggctcc ataagcagta tggagtcgaa
     2161 ccggtccaat tgaagcagat cacgaccgac tccggctcgt tcggagccgc ggtcccgact
     2221 gctttggctt ccgccattct ggaaaagaaa gcgaaattcg cgatcatcag atcccgggct
     2281 ttcgctttcg aattgtcggg caacttcgat ctcccggtcg aaggaaccgg cgtaaccgga
     2341 tattggatct ccggggaagt ggacgattcc gatgccaact tggtaacggc gtccgagccg
     2401 actttagtga aaaagtcttt gactgatcac tatttggcgg ccctcgtgaa agtctcttgg
     2461 aaattgctcc aaacttccga ctataatttg gtcaatttca tcgcgtcttt ggccggcaaa
     2521 aaactggccg agacggagga aacggccttt atcgccggaa gcggaaccgg acagccgaaa
     2581 ggcatccgga gcgaatctct cacgagcgtc gcacaggaag gcgcgggctt ggcttaccag
     2641 gaccttgtca acttgtattt cttgctcccg gcgcagtatc ggcagaatgc ggtattcatc
     2701 acttccgcga tgggcgctaa agccatcgtc ggtttgaagg attcgcagga tcgcccgatc
     2761 ttcgcgcccg gacagccgct cgacgagtta ttccgaaagc ccctccttga atccggagat
     2821 attcccgaaa atctcgggac cggtcaggac acgaccgaag tgtatttcgg cgatccttac
     2881 tactactgga tcaaggatgg acaggctctt tcgatggcga cgcaagatgt gatcgagcgc
     2941 cttcaaacga aggtcttggt ctatcaagcg gtcgatggca aactcgtcct cacggatgcg
     3001 tttgtgaaat tgactggcgt caagccggcc gtcgaatcct aaccttcgcg gattctctct
     3061 cctcgcttca ttccggaaga agcgaggtcg agagtccatg aaaatataaa aatttctatg
     3121 aacagaatca ctattaaatt tttggtcgac tatactccct ataaaactgg agagatcgcg
     3181 actttctttg aaagagaagc gaaccggctg atcgatcggg gctttgcgat gaagtatgtc
     3241 gcgcccgggc aaccggccga ctcggccgcc gaaaaattga aggcgctctc aaacacttcg
     3301 acgagatcca tcccggccgc gccggaaacg aaagtcatcc cgggaccgga aaaaacaaag
     3361 gctccggaag gtccgaaaga tccggaaaaa gtcgaggctc cacaatcccc tataaacggg
     3421 ggcaacgctc aaaccgatga aggatcgggt caagcgccca aagtggcttc aaaagtggaa
     3481 ggaaaggcca aaaacaaggg gcgcggaagg cagtcgggaa agaataaagg caaaaaacaa
     3541 gcatgaatct cacaactctc gcaaaagtta aaagtcatct cgggatccgc gcttccaata
     3601 ccacgaagga cgcgatctta aatcagatca tcaccggcgt ctccggtttc gtcgagcaga
     3661 taacgaatcg aaaattcggc atccaaacct acacggagcg gatcgatgga gacgattcag
     3721 atgaaatttt tttgaaacaa tatcccgtcg tcgatctgat ctctttggag atcgatgacg
     3781 aagcgatcgg cgtggatgct gaagaagaag atgaaacgct gatcgtcgat aatgagatcg
     3841 ggtcgatttg gagagatctc ggatttgcgg aaggccgcaa aaatatcaaa gtcgtctata
     3901 cggccggtta taatcctccg gtcgatacgg atgaatcaag cgacggatcc gatgaagatt
     3961 ccggcgccga aagtttcccg aattcactcg agggagccgt gatccggatg gtcgcccggg
     4021 tctatgaacg ccgcacggcc gagggagtct cgagcgtttc gcccggatcg ttctcggtcc
     4081 aatataaaga ttttatcgac gacgacattc agcagatcat cgattcgcac aagaagcgtc
     4141 gcgtctctta aaacatgtta aattttcgat cgttttcaat ttaccggctt acccgggacg
     4201 agaaatcggg atcttatgga ctcgattttg tcgagactgg cgaatcggtt tcgggatttc
     4261 tcgaaaacgc ttctccggaa ttcgcggcga tcgccggcgg cgcatacgga aagatcttct
     4321 cgcttttctc cgatgatttc gatgtcgatg ttaagacttc agatcgtttg atcgatgaga
     4381 atgatccggc cataaaattc gatgtcagag gagtcttgat cgatgaagat gcgcccggcc
     4441 ggaaacttca gatcacgctc tcgcaagcga tcaagcaata aaatggaact gaaactccaa
     4501 cttgaagggg atgtcctcaa aaaaattaaa gtcgcaaacg aaaagactcg tcaacttttc
     4561 aaggatgccg tccgggacgg atcgacgctg atgatggaat cggctatcag atcgacgccg
     4621 gcctcaaccg gcaacttgcg gaaatcgatt cgacgggaaa tctccggatc cggattgaga
     4681 gcgacgatct ttccttccgt ggtctatggc gcattccttc atggagaatt tcaaggaggc
     4741 ccggcatatt caaagccctt cacaattccg gcccgggaag cgagagaagg cggaacgctt
     4801 tatcgatggg ccaaaaagaa aggactgaac ccttacgcgg tccgggcatc gatcgctaaa
     4861 aaaggcatca agcataatcc atttttgaaa gccgccgcgg aggagtcgac tccgggcgtt
     4921 caagcgatat tcgagagagc gctcgataaa ctggccgctt ccctcggaga ttaaaacgac
     4981 atgtattcaa taatccgtca agcacttctc gatcttctta acggagaatc gatcacaaaa
     5041 atcaaacagg cttatcggac cgaagtttcg gagatcagcg cctatccggt ggcgatcatt
     5101 gcgccgtccg agcatgatgc cgactatcat caaacgagtc cgtcgtccaa caaggagact
     5161 tatattttca cgatccgggt catctatccg ttcacggaag gacaggcaac ggcagatctc
     5221 gctcttgagc aagccctcga cgaattgatc ggcgttcttc gggataaaac atgcctcgga
     5281 tcggcggccg actggattaa gccggtcccg ggtcgatggg gatatgagca aagagaagga
     5341 ggggtcatgc ggatcgcaga actaaatgtt caagcggtca aatatgtcgg ttaaaaatca
     5401 aaaaaaaggc gatggtcgcc gcgtcaaagt cgaaacgcgg cagtcccgga acggggggac
     5461 catcgtctcg gtaaaattca aactcccggc cggaaaatta ttcgctaaaa aataaatata
     5521 aagacatgat acaaagagga gaagaaatca acatgggagt cggcctcgag gatgctcgag
     5581 gaaccggagt cgcgccgcaa gtgtggattc ccggccgaac cccgtccggc gttgtgccgg
     5641 ttgtggaaaa agtcgcgctg aaagaaacgc gcggctcaaa gatcgcttcc cacggagccg
     5701 agatcataaa gtcccgggcc gaaggcgatc atgaatttaa tttgcgcgtt cagtcgatcg
     5761 gctatctctt aaaatcgctt ctcggagcgg tttcaagcca agccaaaaat gcgccaaatg
     5821 gcgccgtcta tgatcacact ttctcggtcc ttccgaatga tccggagcat ccgtcattga
     5881 cgatcggaat cgcgcagtca tccggtcaag attacaagta tagcctcgga gtcgtttcga
     5941 atctttcgat cgaaatgacc ccggacgatc tggtcaaggc agtcgcctcc ttcatcgctt
     6001 cagccgaagc ggagcagtcg cctccttatg tcccggcttt ctcggatgat gatgtctact
     6061 tcaggcatca agacatcacg atcaagatgg ccgacgatat gtccggactg gccgccgccg
     6121 ccgcgctgaa agtgaaatcg atgaagatcg atatcccgaa cggaggccgg gccgatcaca
     6181 atgtctccga attgaatccg ggcaatgtcc tcgcgactca attggatccg aaagtcgctt
     6241 tcgaagtcga ttatcagaac gaagatctcc acgattcata taccggcgga gaatacttcg
     6301 cgcttcagat cacgatggag cgtgctgata ttaccatcgg ctcgaatcag catccgaaat
     6361 tggtcatcac cttcccgaag tgttctctct caaagtggac tccgaatcgt ccgatcgacg
     6421 atgtggtccg cgaacaggtc gaggcgatgg tccattacga cgaagatgaa gcgaaagcga
     6481 tctccgtcgt cctcactaac cttctcaccg gatacgaacc ggccgagtca taatccccta
     6541 gaaatttatg agcgaaagag aaacacgcac aatcaaaact cccatctccg ggaccgagat
     6601 cgtcctcaat tcctacatga caggcggaga gatgatggat ctcgagggag tggcggtcgg
     6661 agccggagtc aaatccgtcg atggccgctc c
//
LOCUS       DAOPQT010000009         6513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_12994_length_6513_cov_3.741251, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000009 DAOPQT010000000
VERSION     DAOPQT010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12994_length_6513_cov_3.741251"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2383)
                     /gene="uvrA"
                     /locus_tag="PKG74_00450"
     CDS             complement(<1..2383)
                     /gene="uvrA"
                     /locus_tag="PKG74_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOB89789.1"
                     /translation="MNKDKIIIKGAREHNLKNIDLEIPRNKIIVFTGLSGSGKSSLAF
                     DTIFAEGQRRYIESLSAYARQFLGRLDKPDVDEIEGLSPAISIDQKAHSSNPRSTVAT
                     ITEIYDYLRVLWSRAGTPFCPICGQEIKKMSLEEIVDAAIAFSAPEGRLEKELIVFAP
                     VVRGRKGEYYQMLYDFLSAGYREARIDGDMRSLRDKIRMERYKQHMIEILIDRLPAGL
                     DLQNKDNRLRLSEAVEASLKHAEDTVVFRNADREASFSAKFSCPRDSFSFPEIEPRLF
                     SFNSPYGACEACHGLGVKDIWSDDICETCRGRRLRPEALSVLVENKNISEVTGMSIGE
                     AQGFFRDLGKKMTLKQKEIAEVPLREILARLKFMYDVGLDYLTLDRRAGTLSGGESQR
                     IRLASQIGSRLVGTLYILDEPTIGLHQRDNEKLINTLHELRDLGNSIIIVEHDEETIR
                     SSDYLVDFGPAAGINGGRIVAQGSIPEIFNNKKEDSLTLRYLRKEEKIDLPAKRRGLK
                     DVPTLKLRGVTENNLQDIDVDIPIGRFTAVTGVSGSGKSTLVYDVLYKAVANKLNHAS
                     FKPGALKAIMGLEYLNRVVNIDQSLIGRTPRSNPATYVGAWTDIRELFASTEDARIRG
                     WKPGRFSFNVRGGRCEHCEGHGYIAIEMHFLPTVYVSCDVCKGKRFDRETLEVEYRGK
                     NIHDILEMSIEEAVKFFKDIPWLRDKLKILDEVGMGYVKLGQSATTLSGGEAQRIKLA
                     AELGKRDTRRTLYLLDEPTTGLHFDDVKKLLEVIQRLVDRGNTAVVIEHNLDVI"
     gene            complement(2386..4389)
                     /gene="uvrB"
                     /locus_tag="PKG74_00455"
     CDS             complement(2386..4389)
                     /gene="uvrB"
                     /locus_tag="PKG74_00455"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOB89790.1"
                     /translation="MAFKLISKNKPAGDQPRAIEELVSGLNQGLKEQTLLGVTGSGKT
                     FTVANVVEKVNRPTLVIAHNKTLAAQLCNEFRELFPENAIHYFVSYYDYYQPEAYMPR
                     TDTYIGKEALINDEIDKLRHAATTSLLTRKDVIIVASVSCIYGLGSPEVYEKNILHFK
                     VGDKITRQELMRQFIKMQFHRTNADLKRGTFRLRGDNWEIMPPNEETIYNFRLEQGEI
                     AEILRIDPVAGYEPEKTPSVPEVFISPAKHFITLDKEREIALLNIEKELEERLKYFES
                     KKQFLEAERLERRTRADIAMMREIGYCNGIENYSRHLSGRAEGEPPSTLLDYFPKDFL
                     VVIDESHVTVPQLRGMYEGDASRKKTLIEYGFRLPSAADNRPLTFPEFEKRINQVIYT
                     SATPGEHEKRRSGKIVEQIIRPTGLVDPELIVRPARGQIDDLIPRIRERVENKERVLV
                     TTLTKRMAEDLSSYLEEQKIKVTYLHSDVKTLERIKILTNLRRGAFDVLVGVNLLREG
                     LDLPEVSLVAILDADKEGFLRSETSLIQTIGRAARNVKGQVLIYADSITGSIDRAMTE
                     TERRRKIQLAYNKEHHITPRTIVREVKDILPADELLDLEMKPVPRSEKALEAMLKQKE
                     REMKAAAKELDFELAAILRDEIKVLTEQMKRKPELNKSGLNRK"
     gene            complement(4493..5059)
                     /locus_tag="PKG74_00460"
     CDS             complement(4493..5059)
                     /locus_tag="PKG74_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOB89791.1"
                     /translation="MEKIILASDSFLKKFVFEKTKFDFDVDPADIDESVFDHLNPKDR
                     VREISLKKCETVHNRHPESMIVAADTLTLLGEETIGKPKDMEEAYRMALSQSDKEILA
                     LTGVTLYFPQKGLFTDLSETRITYQKFDRSDLERVFKDNFPLRMASGLGLFIDAPGFT
                     LVKSINGSYTGAYGMPMEIVYKYLHLFD"
     gene            complement(5081..5671)
                     /locus_tag="PKG74_00465"
     CDS             complement(5081..5671)
                     /locus_tag="PKG74_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89792.1"
                     /translation="MRRIIRLILLLSVVAAIAWIWKTNPDSLISFKDRILAKVSLVYE
                     KISNNDSETPEPTAPAEKKIIWCSEKCFWFGRDGKLFEEAPKTEGSLIKTVKTSASAE
                     IKTGDQVLEPEEAENLYRISEIMKKFDFSFGVIDLGDLSLKEAAIEIDSGVKFYFSLK
                     NSPEYSVAVIESLIKSGEIKNLQYVDFRMENRAYYK"
     gene            complement(5676..>6513)
                     /gene="serS"
                     /locus_tag="PKG74_00470"
     CDS             complement(5676..>6513)
                     /gene="serS"
                     /locus_tag="PKG74_00470"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOB89793.1"
                     /translation="LINTEQAAKIASSRFGYIFGDLARLEFALVKLAMDTLTPYGFIP
                     TIPPVMVKPEIMKGMGKQKFLEAGDAFYIGEDNLYLVGSSEHSIGPLHMDEILDEESL
                     PRRYMGFSTCFRREAGSYGKDTKGILRVHQFDKVEMFSFVKPEDSEEEHRFLLAREEE
                     LLGKLKLPHQVMSICTGDMGFGDYRQFDVETWLPGQGKYRETMSCSNTGDFQSRGLNI
                     KYRVKSTGESKYVHTLNATAFAVGRMLIAILENYQTESGEVLVPEVLRDFVGKEKLSI
                     PR"
BASE COUNT         1492 a         1704 c         1564 g         1753 t
ORIGIN      
        1 tgatgacgtc aaggttgtgc tcgataacga ccgccgtgtt gcctcggtcg actaaacgct
       61 ggatgacttc caggagcttt ttgacgtcat cgaaatggag tccggtggtc ggctcgtcca
      121 gaagataaag cgttcttctc gtgtcgcgtt tgccgagttc ggccgccaat ttgatgcgct
      181 gggcttcgcc gcccgaaaga gtggtcgccg attggccgag tttgacgtat cccataccga
      241 cttcgtccaa gatttttagc ttgtcgcgaa gccaagggat gtctttgaag aacttgaccg
      301 cttcctcgat cgacatttcc agaatgtcgt ggatgttttt tccgcggtat tcgacctcaa
      361 gcgtttcacg gtcgaatctt tttcccttgc agacatcgca gctcacgtaa accgtcggca
      421 agaaatgcat ttcgatggcg atgtatccgt ggccttcgca gtgctcgcaa cggcctccgc
      481 gcacattgaa gctgaaccgg ccgggtttcc aaccgcgaat cctcgcgtct tcagtcgaag
      541 cgaaaagctc gcggatgtcg gtccaggcgc cgacataggt cgccggattg gaacgcggcg
      601 tccgtccgat cagagactgg tcgatgttca ccacccggtt caaatattcg agccccatga
      661 tcgccttcaa agctccgggc ttgaagctcg cgtgattcaa tttgttggcg acggccttgt
      721 acaaaacgtc gtagaccaaa gtactttttc cggaaccgga aactccggtg acggccgtga
      781 atcggccaat cggaatatcg acgtcgatgt cttgaaggtt attctccgtc actccgcgaa
      841 gtttcagggt cggaacgtcc ttgagtcccc ggcgtttggc aggaaggtct attttttctt
      901 ctttacgcaa ataccgcaag gtaagtgaat cctctttctt gttgttgaaa atttccggaa
      961 tcgaaccttg ggcgacgatg cggccgccgt tgatgccggc cgccggtccg aaatcgacaa
     1021 gataatccga cgacctgatg gtttcttcgt cgtgttcgac gataatgatg gaattaccga
     1081 gatcgcggag ctcgtgaagg gtattgatca gtttttcgtt gtcgcgctga tggagtccga
     1141 ttgtcggttc gtcgagaatg tagagggtgc ccacgagccg cgaccctatc tgggaagcca
     1201 acctgatgcg ttgggactct ccgccggaca aagttccggc gcgcctgtcc aaagtcagat
     1261 aatcgagtcc cacgtcgtac atgaatttca gcctcgccaa aatctcgcgc aaaggaactt
     1321 cggcgatttc tttttgcttc agagtcattt tttttccaag gtctcggaaa aatccctgag
     1381 cctctccgat cgacatcccc gtcacttcgc tgatattctt gttctccacg agaacgctca
     1441 aggcttcggg tcgcaaacgg cgtccacggc aggtctcgca gatgtcgtcg gaccagatat
     1501 ctttgacgcc caacccgtgg caagcttcgc aggcgccgta agggctattg aaggaaaaaa
     1561 gcctcggctc aatttccgga aaagaaaagc tgtctcgcgg acaggaaaac ttggccgaaa
     1621 agctcgcttc ccggtcggcg ttccggaaaa cgaccgtgtc ttcggcgtgc ttcaaagaag
     1681 cctcgaccgc ttcgcttaag cgcagacgat tgtctttgtt ttgcaggtcg agtccggccg
     1741 gcaagcgatc gattaggatt tcgatcatgt gctgcttgta acgctccatt ctgattttat
     1801 cgcggagcga ccgcatgtcg ccgtcgatcc tcgcttcgcg gtatccggcg gacaaaaagt
     1861 cgtaaagcat ctggtaatac tcccctttcc gaccgcgaac caccggcgcg aaaacgatca
     1921 gctccttttc caatcttcct tcgggcgcgg aaaaagcaat ggcggcgtcg acgatctcct
     1981 cgagactcat ctttttgatt tcctggccgc agatcggaca aaaaggcgtt ccggcccggc
     2041 tccacaaaac cctcagataa tcgtagatct cggtgatggt cgccacggtc gaacgcggat
     2101 tcgaagaatg cgctttctga tcgatggaaa tcgccggaga caaaccttcg atttcgtcca
     2161 catccggctt gtcgagcctg cccaaaaact gcctggcgta agccgacaaa gattcgatgt
     2221 agcggcgctg gccttcggcg aaaatggtgt cgaaggcgag agaagatttt cccgatccgg
     2281 aaagcccggt gaaaacgatt attttgttcc ggggaatctc gagatcaatg ttcttcaggt
     2341 tatgctcccg cgctccttta atgatgattt tgtctttgtt catttttatt ttctgttcaa
     2401 tcccgattta ttcaattcgg gctttctttt catctgttcg gtcaaaactt tgatttcgtc
     2461 cctcaagatc gccgccagct cgaaatcgag ctctttggcc gcggccttca tttcccgttc
     2521 tttttgcttc agcatcgcct ccagggcttt ttccgacctc ggcaccggct tcatttccaa
     2581 atcgagcagt tcgtccgccg gtaagatatc tttgacttcc cggacgatcg tccgcggcgt
     2641 gatgtgatgc tctttgttgt aagcgagctg tattttcctc cggcgttcgg tttcggtcat
     2701 ggcccgatcg atcgatccgg tgatcgaatc ggcataaatc aagacctggc ctttgacgtt
     2761 tctagcggcc cgtccgatcg tctggatgag cgacgtttcg ctccgcaaaa atccttcttt
     2821 gtcggcgtca agaatagcca cgagcgatac ttccggcaaa tccaatcctt ccctcaagag
     2881 gtttactccc acaagcacgt cgaaagcgcc ccgccgcaga ttggtcagaa tcttgatcct
     2941 ttcaagggtt ttgacgtcgc tgtggagata agtcactttg attttctgtt cttcgagata
     3001 agagctcaaa tcctcggcca tgcgcttggt caaggtggtc acgagcaccc tttccttatt
     3061 ttctacccgt tccctgatcc gcggaatcag atcgtcgatc tggcccctgg ccggacggac
     3121 aataagctcc gggtcgacca aaccggtcgg cctgatgatc tgctcgacga tttttccgct
     3181 ccgtcgcttc tcgtgctctc cgggcgtggc cgaagtgtaa atcacctgat tgatccgctt
     3241 ttcgaattcc ggaaaagtaa gtggcctgtt gtcggcggcc gaaggcagcc tgaaaccgta
     3301 ttcgatcaaa gttttctttc tcgaagcgtc cccttcatac atccccctca actggggaac
     3361 ggtcacgtgc gactcgtcga taaccacgag aaaatctttc ggaaaataat cgagtaaagt
     3421 cgaaggcggt tcgccctcgg cccggccgga aagatgacgc gaataatttt cgatgccgtt
     3481 gcaatatccg atctccctca tcatggcgat atcggccctg gtgcggcgct ccaatctttc
     3541 ggcttccaag aactgttttt tggattcgaa atacttcaat ctttcctcga gttctttttc
     3601 gatattcaac aaagcgattt ctcgttcttt gtccagagta atgaaatgct tggccggaga
     3661 aatgaatact tccggaaccg acggtgtctt ctccggctcg tatccggcga ccggatcgat
     3721 tctcaaaatc tccgcgattt cgccctgctc cagccgaaaa ttgtagatcg tttcctcgtt
     3781 cggcggcatt atttcccagt tgtctccgcg aagcctaaaa gtgccccgct tcaaatcggc
     3841 gttggtgcga tgaaactgca ttttgatgaa ctgtctcatc agttcctgcc gggtgatctt
     3901 atccccgacc ttgaaatgca aaatattttt ctcgtagacc tccggacttc ccaatccgta
     3961 gatgcaggaa accgaggcca cgataataac gtctttccgc gtcagcaaac tcgtggtcgc
     4021 cgcgtggcgc agtttgtcga tctcgtcgtt gatcaaagct tctttgccga tgtaagtatc
     4081 ggtccgcggc atataagcct cgggctggta ataatcgtaa taggaaacga aataatggat
     4141 cgcgttttcc gggaaaagct cccggaattc gttgcaaagc tgggccgcca gggtcttgtt
     4201 gtgggcgata accaaagtcg gccgattgac cttttcgacg acattggcca cggtaaaggt
     4261 ctttcccgac ccggtaacgc ccaaaagggt ctgctctttg agaccctgat tcaagcccga
     4321 aaccagctcc tcgatcgccc ggggctggtc tccggccggt ttgtttttgg aaatcaattt
     4381 aaacgccatt tgaacagcaa aatttccccc ggttccgctt acatataagg gggtatagag
     4441 tgtttatacc acaaaaatag ctaaaaagca aagggatcct tttaccaaag ctttaatcaa
     4501 aaagatgtaa atatttatat acgatttcca taggcatgcc gtaagctccg gtatatgaac
     4561 catttatgga tttaactaag gtaaagccgg gcgcatctat aaatagaccc aagccgcttg
     4621 ccattcttaa cggaaaatta tccttaaaaa ccctttctaa atcgctacga tcgaactttt
     4681 ggtatgtaat tcttgtttcc gacaaatcgg tgaacaatcc tttttgagga aaatataaag
     4741 ttactccggt caacgcgaga atttctttat cgctttgact taacgccata cgataagcct
     4801 cctccatatc ctttggttta ccgatagttt cttcaccgag aagggttaag gtgtctgcgg
     4861 cgacaatcat cgattccgga tgcctattgt gaacagtttc gcattttttc aacgagattt
     4921 ctcgcactcg atctttagga ttcaaatgat cgaaaacgga ctcgtctata tcggctgggt
     4981 cgacatcgaa atcaaatttc gttttttcga aaacaaactt tttgagaaaa gaatcggatg
     5041 ctaaaattat tttttccata atggggaaaa tggataaaat ttacttataa taagcccggt
     5101 tttccatccg gaaatcgaca tattgaagat tcttgatttc tccggacttg atgagcgatt
     5161 cgataaccgc gacgctatat tccggcgagt tcttgaggct gaaataaaac ttgactccgg
     5221 aatcgatttc gatcgccgcc tctttgagcg acagatcccc gagatcgatg acgccgaagc
     5281 tgaaatcgaa tttcttcatg atttcggaaa tccggtaaag attttccgcc tcttccggtt
     5341 cgagcacctg gtctccggtt ttgatctccg ccgaagcgga agtcttcacg gttttaatca
     5401 gacttccttc ggttttcgga gcctcctcga aaagctttcc gtcgcgtccg aaccagaagc
     5461 acttttccga acaccaaatg atcttttttt ccgcaggagc cgttggttcg ggtgtctcgg
     5521 aatcgttatt cgagattttt tcgtaaacaa gagatacctt cgccaaaata cgatctttga
     5581 acgagatcag ggaatccgga tttgttttcc aaatccaggc gattgcggca acaacgctta
     5641 gaagcaatat gagccgaatg atccggcgca ttgttttatc tgggaatgga taatttttcc
     5701 tttccgacaa aatcccgtaa aacttccggc accagaactt ctccggattc ggtctggtag
     5761 ttctccaaaa tggcgatgag catccgaccg acggcgaagg cggtggcatt caaagtatgg
     5821 acgtatttcg attctccggt cgatttgacg cggtatttga tattcagacc gcgcgactgg
     5881 aaatcgccgg tgttcgaaca agacatggtt tcacggtact tcccttgtcc gggaagccac
     5941 gtttccacgt cgaactgccg ataatccccg aatcccatat ctccggtgca aatggacatt
     6001 acctggtgag gaagtttgag cttacccaaa agctcttctt cccgcgcgag gagaaatcgg
     6061 tgttcttctt ccgaatcttc gggtttcaca aaagaaaaca tttccacttt gtcgaattgg
     6121 tgcacccgga gtattccttt ggtgtctttg ccgtagcttc cggcttcgcg gcggaaacaa
     6181 gtggaaaatc ccatgtagcg cctgggtaac gactcttcat ccaatatttc gtccatatgc
     6241 aaaggaccga tcgaatgttc ggagctaccg acgagataaa gattgtcttc cccaatataa
     6301 aaagcgtctc cggcttccaa gaatttttgc ttgcccattc ctttcatgat ttcgggtttg
     6361 accatcaccg gaggaatggt cgggataaag ccgtaaggcg taagcgtatc catggcaagc
     6421 ttcaccaaag cgaattccaa tctcgccaaa tctccgaaaa tatacccgaa cctgcttgaa
     6481 gcgatcttgg ccgcctgttc ggtgttgatt aat
//
LOCUS       DAOPQT010000010         6501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_13025_length_6501_cov_3.390320, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000010 DAOPQT010000000
VERSION     DAOPQT010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13025_length_6501_cov_3.390320"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="PKG74_00475"
     CDS             <1..1321
                     /locus_tag="PKG74_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOB89794.1"
                     /translation="IAMAENTDFRNGIEIPQALSTTIADFGKKIQDLNERKASASEFL
                     ETIIASGLGLESSDIHFETEEKETRLRIRVDGVLNDIARINKDSYALILNRIKLLGGL
                     KLNIRDAAQDGRFTARVSDPGIDVEIRVSVNPAKFGETAVLRLLNPKTISLDVSDLGL
                     RKDDESLMTNELKRPNGMILVTGPTGSGKTTTLYAFLKAVSNPDIKIVTIEDPIEYRL
                     PGIEQTQVDETAGYTFENGLRSILRQDPDVILVGEIRDEETAEIALHSSLTGHLVFST
                     LHTNNAVGAVPRLIDLGIKPAVIGPALNVVVSQRLVRRICEKCREPLEPDEGLESRIK
                     EFLAALPQKTDKPEFEEIRLYRAIGCEECNNTGYKGRIGIFEIVGFDKAFASIINEKT
                     TEIDIEKAALERGFVGIQADGLIKVLRGVTTIEEVERQTGPVDWKIV"
     gene            1525..2511
                     /gene="ruvB"
                     /locus_tag="PKG74_00480"
     CDS             1525..2511
                     /gene="ruvB"
                     /locus_tag="PKG74_00480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOB89795.1"
                     /translation="MKKANLQLSKNDDLNVDLLLRPRIFEDYVGQETIKKALSVILGA
                     AKQRNEAPDHLLFYGPAGLGKTTLAHLVAFDLGAQIKETSGPALEKTGDLAAVLTNLE
                     PRDVLFIDEAHRLSKPIEEVLYPAMETRKLNIVLGKGPAARTISLDLPPFTLIAATTR
                     VNMLSNPLRSRFGASFRLDYYSLENIESIIEKSAKILDIGIEESGIARLAAASRFTPR
                     IANRLLKRSRDFAEMNKIQMIDGQTIKKVLELLDIDEFGLEYIDRKFLGIIENQFKGG
                     PVGLNTLSAALGEEKGVIEEVYEPYLMKIGFLKRTSGGRVVTDAAKEHLARF"
     gene            2512..2964
                     /gene="tsaE"
                     /locus_tag="PKG74_00485"
     CDS             2512..2964
                     /gene="tsaE"
                     /locus_tag="PKG74_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOB89796.1"
                     /translation="MTVIRTESLEQTRDLAAGFAEKISKNRKNKNRALVLLLSGDLGS
                     GKTSFVQGALRYFGIKRVLSPTFVLMKHYRIPRAKNNIKDIYHVDAYRLKSFADLKTL
                     GFDKVLESGKSLVFIEWPERLRTRVLKNSLKIKFSFGEKENERLIKLP"
     gene            2969..3847
                     /locus_tag="PKG74_00490"
     CDS             2969..3847
                     /locus_tag="PKG74_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOB89797.1"
                     /translation="MPDKILLLIDANSLIYRSFFALPPFTAPDGRPTGALYGLANSLL
                     KILKEIHPEYVLAAYDRPEPTFREQEFEDYKITRPATPSDLVSQLKESKAMMEQFNIM
                     TYEMPGFEADDIIATLTQKYKSRDDLKIAILSGDHDTLQLVEDPKVNAWIPQKGANNF
                     TVYDEKTVIEKYGVRPDQIPDYKALVGDKSDNIPGVSGIGPKTAVGIISEFGSIEDFY
                     GLSPAPINSSYKKLIAGKQMAVLSKRLATVRRDVPLPEVDLSGLKFGIVSKKELMDYF
                     TSLGFHSLASRLEMIE"
     gene            3855..5189
                     /locus_tag="PKG74_00495"
     CDS             3855..5189
                     /locus_tag="PKG74_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOB89798.1"
                     /translation="MNKIFSQIKEWWRSPQMTGFWFFLFAGIFSLVIGLRYFYGIWFW
                     VNFVIVLISGVFIFRAGHRAARESRLAAFGYRRLQSIVSNLSDGIVSYDNNMEITEMN
                     AAAEQIFNIEKELVIGKRISPEWGGDPKLKMIAQVIFPSLAPTVIKRSDPGVYPQVVD
                     ISFVEPDIDLRVSTDRITGEKGEVLGFVKVVHDRTREIKLLRSKSEFITVAAHQLRTP
                     LTAVRWALENISSVKELDSATKELVSTGLSASGKLMKIIEDLLNTSKIEEGRFGYEFR
                     DMDIVAFLEEALSGVQAIAKEYGVNLYFDRPANGVPIIVRMDPSRLGMAFSNLLDNAV
                     KYNVKNGSVTVKIERMTDRPYVEVSITDTGVGIPAEDMPKLFSKFYRGANVTRDETDG
                     TGLGLYIAKNIIFRHGGGIQAESVLNRGTTVHVFLPTDPKLIPPKEIIYGEE"
     gene            5200..6060
                     /gene="mutM"
                     /locus_tag="PKG74_00500"
     CDS             5200..6060
                     /gene="mutM"
                     /locus_tag="PKG74_00500"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOB89799.1"
                     /translation="MPELPEVQTVVDDLNRKIKNRKITGVWTDARKIVRGSFPDFRKS
                     VIGRKILRVERRAKNILIYFDSGKLMLTHLKMTGHYLVGRWDLRKPKPTPVSDGRLSE
                     KVNAYIHVVFVLDDGRMLALSDLRKFAKIMFGDVDDICASLKDLGPEATEISFKDFDR
                     LLEKSRKPIKKLLMDQNLIAGIGNIYSDEILFDAKIHPLSLASSLNPERRKRLYRSIK
                     KILKKALELRGTSVSDFRDTSGVEGDYGLRRLVYRREGKLCPGKCPGKVKRIKIGGRS
                     SYFCSDCQKL"
     gene            6091..>6501
                     /locus_tag="PKG74_00505"
     CDS             6091..>6501
                     /locus_tag="PKG74_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89800.1"
                     /translation="MVLLFYGRDAFRRKQKIDEILGAYRDKRGNLGVFKADLEDNIEA
                     FDEAIDFLKNQSLFDPLKIVVFKNFSSSLFKASKRTKDLKELINLHKDSKDTLIFSAE
                     TKPTADFKFFFDEEVKSQEFEELDDAGILKFIKSE"
BASE COUNT         1774 a         1623 c         1612 g         1492 t
ORIGIN      
        1 gattgcaatg gctgaaaata cggatttccg gaacggaatc gagattcccc aagcgctttc
       61 tacaaccata gccgattttg gaaagaaaat tcaagatttg aacgagagaa aagccagcgc
      121 ttccgaattt ttggaaacga tcatcgcttc cggcctcgga ctcgaatcct cggacatcca
      181 tttcgagacc gaagaaaagg aaacgcgctt gagaatccgc gtcgacggag ttttgaacga
      241 catcgcgaga atcaataagg attcctacgc cctgattttg aaccggatca agcttttggg
      301 aggcctgaag ctgaatatta gggacgcggc ccaggacgga cgtttcacgg cccgcgtttc
      361 cgaccccggg atcgacgtcg agataagggt ctcggtcaat ccggcgaaat tcggggaaac
      421 cgcggtcttg aggcttttga acccgaaaac catttctttg gatgtttcgg atctcggact
      481 ccgcaaagac gacgagtctt tgatgacaaa cgagctgaag cggccgaacg gcatgatttt
      541 ggttaccgga ccgaccggat caggcaaaac caccactctt tacgctttcc tgaaggctgt
      601 ttccaatccg gatatcaaga tcgtcaccat cgaagatccg atcgaatacc gtcttccggg
      661 aatcgaacag acccaggtcg acgaaacagc cggttatact tttgagaacg gtttgagatc
      721 gatcttgcgg caggatccgg acgtgatcct ggtcggcgag atccgcgacg aagaaacagc
      781 cgaaatagcg cttcacagca gcttgaccgg acatttggtt ttttccactc tccatacgaa
      841 taacgccgtc ggcgccgtgc cgaggctgat cgatttggga atcaagcccg cggtcattgg
      901 cccggctttg aacgtggtcg tgagccagcg cctggtaaga aggatttgcg agaaatgccg
      961 cgagccgctt gaaccggatg aaggccttga atcccggatc aaggaattct tggcggcatt
     1021 accccaaaaa acagacaaac ccgaattcga agaaatccgg ctctataggg ctattggctg
     1081 cgaagagtgt aacaacaccg gatacaaagg acggatcggt attttcgaga tcgtcggatt
     1141 cgacaaggca ttcgcctcca tcattaacga aaaaaccacg gaaattgata tagaaaaagc
     1201 cgctctcgag cgcggctttg tcggcatcca ggccgacgga ttgatcaagg ttctccgggg
     1261 cgtcaccacc atcgaagaag tggagcgtca gacaggaccc gttgactgga agatagttta
     1321 gaaaacaaaa aatccgcgag ggatcgtcgg caagctttaa taaagattaa tctttctttc
     1381 aagaatagaa agaaagattt agaattgttt cgaggcgtag tccagccaaa gccaaacagg
     1441 ccaaaagcta aaaacaattc ccatcgccga atatcctttg cggatttaac tcaagatagc
     1501 atatatgggg gtttctgtca agagatgaaa aaagccaatc ttcagctgtc gaaaaacgac
     1561 gatttgaacg tggatttgct tcttcggccg cggattttcg aggattacgt cggccaggaa
     1621 acgatcaaaa aggctttgtc cgtgattttg ggagcggcga agcaaagaaa cgaagcgccg
     1681 gatcatcttt tgttctacgg tccggccgga ctcggaaaaa caaccctcgc ccatttggtg
     1741 gcttttgatc tcggcgccca aatcaaagag acttcgggcc cggctttgga aaaaaccgga
     1801 gatttagccg cggttttgac caatctcgaa ccgcgggacg tgcttttcat cgacgaagcc
     1861 catcgtttga gcaaaccgat cgaagaagta ctttatccgg ccatggaaac ccgcaaactg
     1921 aacattgttt tgggcaaagg cccggcggcg agaacgatct ctctggattt gccgccgttc
     1981 accttgatcg cggccacaac cagggtgaat atgctttcga atcctttaag atcgcgtttc
     2041 ggcgcttctt tcaggctgga ttattattct ttggaaaaca tcgagtcgat catcgaaaaa
     2101 tcggcgaaga tcctagatat cggcatcgaa gaatcgggaa tcgcgagact ggccgcggcc
     2161 agccgtttca ctccaagaat cgcgaaccgg ctcttgaaaa gaagccggga tttcgccgag
     2221 atgaacaaga tccaaatgat cgacggacaa acgatcaaaa aagtgctcga gttgctcgat
     2281 atcgacgaat tcggactcga atacatcgat cgtaagttct tgggaatcat cgaaaaccag
     2341 ttcaaaggag gtccggtggg cctaaatacc ctttccgcgg ccctcggaga agaaaaagga
     2401 gtgatcgaag aggtttacga gccgtatttg atgaaaatcg gctttttgaa aagaacttcc
     2461 gggggccggg tcgtcaccga tgcagccaaa gagcatctgg ccaggtttta aatgactgtt
     2521 ataagaaccg aatcgttgga acaaacccgg gatttggccg cgggtttcgc ggaaaaaatc
     2581 tccaagaacc ggaaaaacaa gaatcgggcc ttggttttac tcctttccgg agatctcggg
     2641 tcgggcaaaa cttcttttgt tcagggagcg cttcggtatt tcgggatcaa acgcgttttg
     2701 agtccgacat tcgttcttat gaagcattat cggattccgc gagcgaaaaa caatatcaaa
     2761 gacatttacc acgtcgacgc ctatcgtctg aaaagcttcg ctgatcttaa aaccctcggc
     2821 tttgataaag ttctcgaatc cggcaaatcc ttggttttca tcgaatggcc cgaacggctg
     2881 cgaacccgag ttttgaaaaa ttctcttaaa atcaaatttt ctttcggaga aaaagaaaac
     2941 gaacgcctaa tcaaacttcc gtaaaaccat gcccgacaaa atattgcttc tcatagacgc
     3001 caattccctg atttaccgga gctttttcgc tttacctcct tttaccgctc cggacggccg
     3061 accgaccgga gctctttacg ggctggccaa ttcgcttttg aaaatcctta aggaaataca
     3121 tccggaatac gttttggcgg cttacgaccg gcctgaaccg actttccgtg agcaggaatt
     3181 cgaagactac aagatcaccc ggccggcaac accctcggat ttggtaagcc agctcaagga
     3241 atccaaagcg atgatggagc aattcaacat catgacctac gaaatgcccg gattcgaagc
     3301 ggacgacatc atcgccacct tgacccaaaa atacaaatcc cgcgacgatc ttaaaatcgc
     3361 cattctttcc ggagaccacg acaccctgca gcttgtcgaa gacccgaaag taaacgcctg
     3421 gattccccaa aaaggcgcga acaacttcac ggtttacgac gaaaaaaccg ttatcgaaaa
     3481 atacggggtt aggccggatc agataccgga ctacaaagct ttggtcggcg acaaatccga
     3541 caacattccg ggagtatccg gcatcggccc caaaaccgcg gtcggaatca tcagcgaatt
     3601 cggctcgatc gaggattttt acggcctttc cccggcgccg atcaattcga gctacaaaaa
     3661 attgatcgcc ggcaagcaaa tggccgttct ttcgaaaaga ctggccacgg tgaggcgtga
     3721 cgttcctctg cccgaagtcg atctttcggg actgaaattc ggtatcgtct ccaaaaaaga
     3781 attgatggat tatttcacga gtctcggatt ccattcttta gcctcgaggc tcgaaatgat
     3841 agaataataa ggatttgaac aaaatctttt cgcaaatcaa agagtggtgg cggtcgccgc
     3901 aaatgaccgg cttttggttt tttcttttcg ccggtatttt cagcctcgtc atcggactac
     3961 gttattttta cggcatctgg ttctgggtaa atttcgtcat cgttcttatc tccggggttt
     4021 tcattttccg ggccggccac agagccgcga gggaaagccg tttggcggct ttcggttacc
     4081 gccggctcca gagcatcgtc tccaatttga gcgacggcat cgtgagctac gacaacaaca
     4141 tggagatcac cgagatgaac gccgccgccg agcagatctt caatatcgaa aaagaacttg
     4201 tcatcggaaa gagaatctct ccggaatggg gcggcgatcc gaaactgaaa atgatcgccc
     4261 aggtgatctt cccctctctc gctccgacgg tcataaaaag aagcgacccc ggagtttacc
     4321 cgcaggtcgt cgacatttct tttgtcgagc cggacatcga tctccgggtc tcgaccgaca
     4381 ggataaccgg agaaaaagga gaagtcctcg gattcgtcaa agtggtccac gaccggacaa
     4441 gggaaataaa acttttgcgc tccaaatcgg aattcatcac cgtggccgcg catcagctcc
     4501 gaacgccttt gacggccgtg cgctgggctt tggaaaatat ttcctcggtc aaagaacttg
     4561 attcggcgac aaaagagctt gtttcgaccg ggctttccgc ttccggaaag ctcatgaaaa
     4621 tcatcgaaga ccttttgaac acctcgaaga tcgaagaagg ccgttttggc tacgaattcc
     4681 gggacatgga tatcgtcgcc tttctcgagg aagcgctttc cggagtccag gccattgcca
     4741 aagaatacgg ggtcaatctt tattttgacc gtccggcaaa cggcgtgccg ataatcgtga
     4801 ggatggatcc ttcgagactc ggcatggctt tttccaatct tttggacaac gccgtcaaat
     4861 acaacgtcaa aaacggcagc gtcaccgtga aaatagaaag aatgaccgat cggccttacg
     4921 tcgaagtgag tattacggat accggcgttg gcattccggc cgaggacatg cccaagcttt
     4981 tcagcaagtt ttaccgcgga gcgaacgtga cgcgtgacga aaccgacggc accggactcg
     5041 ggctctacat cgccaaaaac atcattttcc gccacggcgg cggcatccag gccgaatcgg
     5101 ttttgaaccg aggcaccact gtccatgttt tcctgccgac cgatccaaaa ctcattcctc
     5161 cgaaagaaat catctacggc gaagaataaa tcctaaataa tgcccgaact tccggaagtg
     5221 cagacggtcg tcgacgatct gaacagaaaa ataaagaatc ggaaaatcac aggtgtctgg
     5281 acagacgccc ggaaaatcgt aagaggatct tttccggatt tcaggaaaag cgttatcggc
     5341 cggaaaatct tgcgcgtcga gcgtcgggcg aaaaacatcc tcatttattt tgattccggg
     5401 aaattgatgc tgacccacct caaaatgacc ggccattatc ttgtcggccg ctgggatttg
     5461 cgaaagccaa agccgacacc cgtatccgac ggccggctct cggaaaaagt gaacgcctat
     5521 atccacgtgg tttttgtttt ggacgacggc cggatgctcg ctttgtccga tctccgcaaa
     5581 ttcgccaaaa ttatgttcgg cgacgtcgac gatatttgcg ccagcctcaa agacttgggg
     5641 ccggaggcga cggagatttc ttttaaggat ttcgaccggc ttttggaaaa atcgcgcaag
     5701 cccatcaaaa aactgttgat ggatcagaat ttgatcgcgg gcataggaaa catctattcg
     5761 gacgaaattc ttttcgacgc caaaatccat cccctgtctt tggccagctc tcttaatccg
     5821 gaacgaagaa aaagacttta tcgttcgatc aaaaaaatat taaagaaagc ccttgagctc
     5881 cgcggcacgt cggtctcgga tttcagggac acttccggag tcgaaggaga ttacggcttg
     5941 cggcgcctgg tttatcgtag ggagggcaag ctttgcccgg gaaaatgccc gggcaaagtc
     6001 aaaagaatca agatcggagg ccgctcttct tatttctgct ccgactgcca aaagttgtaa
     6061 gaagctccgt ctcttcggta tcataagctc atggttttgc ttttttacgg ccgcgacgct
     6121 tttcgccgca agcaaaagat cgacgagatc ctgggggctt accgcgacaa acgcggaaac
     6181 ttgggggttt tcaaagccga tttagaggac aatatcgaag ctttcgacga ggcgatcgat
     6241 tttctgaaaa accagtctct tttcgatcct ttgaaaatcg ttgtttttaa gaacttttca
     6301 tcgagccttt tcaaggcctc gaaaagaacc aaagacctga aagaactgat caatctccat
     6361 aaagattcca aagataccct gatcttttcg gccgaaacaa aaccgacggc ggatttcaaa
     6421 ttctttttcg acgaagaagt aaagtctcaa gaattcgagg agttggacga tgccgggatc
     6481 ttgaaattca taaagtccga a
//
LOCUS       DAOPQT010000011         6241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_13733_length_6241_cov_3.108147, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000011 DAOPQT010000000
VERSION     DAOPQT010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13733_length_6241_cov_3.108147"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKG74_00510"
     CDS             <1..499
                     /locus_tag="PKG74_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89801.1"
                     /translation="NELRPMIDYYNGQLRIGVHLMVDKPEAIIESWLMAGVKEAAVHL
                     ERLADRNFFKYVCGEYNAEPVIAVSPETSARTALGYVDDFKKLLILGVKPGLSGQEFQ
                     PAVLEKVTEIKRAAPEVIVMVDGGVNLNNIKSIAEAGADIALSGSYIFNAGDPAKAYA
                     DLSNV"
     gene            492..1346
                     /locus_tag="PKG74_00515"
     CDS             492..1346
                     /locus_tag="PKG74_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOB89802.1"
                     /translation="MFELTDEKIKFLEKRANEIRESVVKMLYEAGSGHSAGPLGMADV
                     FSAFYFHILNHKPENPDWPDRDRLVLSNGHICPVRYAAMAYAGYFPLDELNTLRKLGS
                     RLQGHPHRQSLPGTETTSGPLGTGLSQAIGMALAAKLDKKKYRVYCFVSDGEQNEGNH
                     WEAVMFAGKLKINNLTMLMDRNNIQIDGFTEDVMPLEPLREKYESFNWRVMEINGHSI
                     EEIVDSVNEAKTIYEKPTLIIAHTIPGKGVSFMENDYAWHGKPPTKDQALAALKELRS
                     LGGKIEME"
     gene            1360..1701
                     /locus_tag="PKG74_00520"
     CDS             1360..1701
                     /locus_tag="PKG74_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89803.1"
                     /translation="MNILRIIKIIYLYIFSFVGLVLIITASVRLVNLALKTFIFTEVD
                     QYYIYPSAKPIRENEEYVEPDPEELAAFNERQLKQQRQRDASDSLAMLIIGVPLYSYH
                     WKLVTKKKDEE"
     gene            1705..2703
                     /locus_tag="PKG74_00525"
     CDS             1705..2703
                     /locus_tag="PKG74_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOB89804.1"
                     /translation="MLNPELYLNPDIFSENPEKAALRDGYGLGLLEAGSENSDVVALS
                     ADLTESTRAEAFAAKYPERFFQVGVAEQNMVGVAAGLGLSGKIPFISSYAVFSPGRNW
                     EQIRTTICYNDSNVKIAGHHAGVSTGPDGATHQATEDIALMRVMPNMKVFVPCDAIEA
                     KKATAAVAKIWGPAYLRFARNATPIMTTEKTPFVPGKAEIFWQPGNGKKPEVVIIGCG
                     PLVYNCLLAAKNLEKQKVHVLVINCHSIKPLDNRTIIAATKKCGAVVTVEEHQIAGGL
                     GSVVAEMLSEEHPTPIEFVGMRDTFGESGKGEELIKKYGMAPNHIEKAVQKVLKKK"
     gene            2715..3917
                     /locus_tag="PKG74_00530"
     CDS             2715..3917
                     /locus_tag="PKG74_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOB89805.1"
                     /translation="MLLGRLKATIIFRYSPGFTLVELLLVMGILGVLTGVTILVLNPA
                     ELLKQTRDSERISDMKSLTQLFTVYNTQVSELYGDPKDVYLSLPDSEPDCSSYDLPDL
                     PTGYGYHCVAETDLRKVNGSGWIPVDFTSIPGGSPLSVLPVDPVNDFLHYYTFLKGGS
                     YKLSTEMESIAYGPAGSNPVTIKDNGINSFLYEVGTNLTLAAPANILINSEIDNLVSG
                     YTPGWDTDLNGTYRPATGFSGGYNSGVASPNIGYHAHAKPDCGINESGCLEYIDENCA
                     YDNCHRWLGVTQTWSNPGVLKGWTTGTKIRVRFLAKKNVTNKYPHFGLHHYSISLGAN
                     TFAGGGGTISAPLPQIGKWTVVTGEFTVTPDWELTTHGVTLYLYGYSGADEGRIWIDS
                     IDAVYWNP"
     gene            complement(4795..5520)
                     /locus_tag="PKG74_00535"
     CDS             complement(4795..5520)
                     /locus_tag="PKG74_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOB89806.1"
                     /translation="MDKILLEIRAGAGGDEASIFAGDLARMYQRYAVKHGFKFQTIDA
                     NDTSLYGIKSMVAQIQGPDVYEIFKHESGVHRVQRIPKTEKSGRVHTSTASVAVLPIV
                     DPGKVQINPGDLDVVFFRSSGPGGQNVNKVETAVRITHRPTGIVVSSQVERSQYANKE
                     RAMESLAAKLYEQKQKEAVGEVSELRREQIGTADRSEKIRTYNFPQDRITDHRLNKSF
                     HNIEKIMEGDMDKIVEAFRKNKE"
     gene            complement(5618..5941)
                     /gene="rpmE"
                     /locus_tag="PKG74_00540"
     CDS             complement(5618..5941)
                     /gene="rpmE"
                     /locus_tag="PKG74_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOB89807.1"
                     /translation="MKQDIHPKYYPEAAITCACGKVWKIGGTLEKAEIEICSACHPFY
                     TGQEKLLDTAGRVEKFKAKKAKAEATPKKVKKIRVKKAVVEKTVKPKAKPKTKAAAKP
                     KTAKK"
     gene            6182..>6241
                     /locus_tag="PKG74_00545"
     rRNA            6182..>6241
                     /locus_tag="PKG74_00545"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1536 a         1525 c         1562 g         1618 t
ORIGIN      
        1 gaacgaactc cgaccgatga tcgattatta caacggtcag cttcgaatag gtgttcatct
       61 catggtcgac aagccggagg cgatcatcga aagctggctc atggctggcg tcaaagaagc
      121 ggcggtgcac ctcgagcggc tagccgaccg gaatttcttc aaatacgttt gcggagaata
      181 caacgccgag ccggtgatcg ccgtttctcc ggagacttcg gcacggacag ctctcggata
      241 tgtcgacgat ttcaaaaaac tgcttatctt gggagtgaaa cccgggcttt ccggccaaga
      301 gtttcaaccg gctgttcttg aaaaagtaac ggagataaaa agagccgccc cggaagtgat
      361 tgttatggtc gacggcggcg tcaatcttaa taacatcaaa tccatcgccg aggccggagc
      421 ggatatcgct ctttcggggt cgtatatttt taacgccgga gatccggcca aagcttacgc
      481 cgacttgtcc aatgtttgaa ctgaccgacg aaaaaataaa gtttttggaa aagcgggcca
      541 acgaaatccg cgaaagcgtc gtcaaaatgc tttacgaagc cggttccggt cattcggccg
      601 ggcccttggg tatggccgat gttttctcgg cgttttattt ccacattctg aaccacaaac
      661 ccgaaaatcc ggattggccg gatcgggacc ggctggtctt atctaacggc catatttgcc
      721 cggtgcgcta cgccgccatg gcttacgccg gatatttccc gctcgacgag ctgaatactc
      781 taagaaagct cggctcccgg ctccagggtc atccccacag gcaatccttg cccggaacgg
      841 aaacgacttc cgggcctttg ggcacgggac ttagccaagc gatcggcatg gctttggccg
      901 ccaagctcga caagaaaaaa taccgcgttt attgctttgt ttctgacggg gaacagaacg
      961 aaggaaacca ttgggaagcg gtgatgttcg ccggaaaact caaaataaac aacctgacca
     1021 tgttgatgga ccggaacaac atccagatcg acggatttac cgaggatgtc atgcccctcg
     1081 agcctttaag ggagaaatac gaatctttca attggcgcgt catggagatc aacggccaca
     1141 gcatcgagga aatagtcgat agcgtgaatg aagccaagac catttacgag aagccgaccc
     1201 tgattatagc ccataccatt cccggaaaag gggtgagctt tatggaaaac gattacgctt
     1261 ggcacggaaa accgccgacc aaagaccagg cccttgccgc tttgaaagaa ttaaggtcgc
     1321 tcggcggcaa aatagaaatg gaataaacaa caaaaaaaca tgaatatcct aagaatcatc
     1381 aagataatct atctctatat tttttccttc gttggattgg tgcttattat caccgcctcg
     1441 gtgagattgg tgaacctcgc cctgaaaact tttattttca ccgaagtcga ccagtattat
     1501 atttatccct cggccaagcc gataagagag aacgaggaat acgtcgaacc ggatccggaa
     1561 gaactggccg cgttcaacga acgccaactg aagcagcagc gtcagagaga cgcttccgat
     1621 tccttggcca tgcttatcat cggcgttcct ctttactctt atcactggaa attggtcacc
     1681 aagaaaaaag acgaagaata aaccatgctt aaccccgaac tgtatctgaa ccccgatatt
     1741 ttctccgaaa atcccgagaa ggcggctttg agggacggat acggccttgg gcttttggaa
     1801 gccggaagcg agaacagcga cgttgtcgct ctttcggcgg atttgaccga atcaacgcgg
     1861 gccgaagctt tcgccgcaaa atacccggaa agattttttc aggtcggcgt ggccgaacag
     1921 aacatggtcg gcgttgccgc cggtctcggg ttgagcggca agattccgtt tatttcttcc
     1981 tacgccgttt tctctccggg ccgaaattgg gaacaaatcc ggacgacgat ctgctacaac
     2041 gattcgaacg tcaagatcgc cggccaccac gccggggttt ccaccggtcc ggacggagcg
     2101 acccatcagg cgaccgagga tatcgccttg atgcgggtca tgccgaacat gaaggttttc
     2161 gtgccctgcg acgcgatcga ggcgaaaaaa gcgaccgcgg ccgttgccaa gatctggggt
     2221 ccggcctatt tgcgattcgc gagaaacgcg accccgatca tgacgaccga aaagactcct
     2281 tttgttccgg gaaaggccga gattttctgg cagccgggta acggcaaaaa acccgaagtg
     2341 gtgatcatcg gctgcggccc gcttgtttac aattgcttac tcgcggccaa aaacctcgaa
     2401 aagcaaaaag tccacgttct cgtcatcaat tgccattcga tcaaaccctt ggacaatcgg
     2461 acgattatcg ccgccaccaa aaaatgcggg gccgtggtca cggtcgaaga gcaccaaatc
     2521 gccggaggtc tgggctcggt cgtggccgaa atgctatccg aagagcatcc gacgccgatc
     2581 gaattcgtgg gcatgcggga tactttcggg gaatcgggga aaggcgagga gcttatcaaa
     2641 aaatacggta tggctccgaa ccatatcgaa aaagcggttc aaaaagttct gaaaaagaaa
     2701 taaccgcttg gggtatgctc cttggccgcc taaaagcaac gatcatcttc cgatattctc
     2761 caggtttcac tcttgtcgaa cttttgcttg tcatggggat tttgggagtc ctgaccggag
     2821 tcaccattct tgtcctgaac cctgcggagc ttttgaaaca aacgagagat tcggaaagaa
     2881 tatccgacat gaagtccctc acccagcttt tcaccgttta taacactcaa gtctctgaac
     2941 tctacggcga tcccaaagac gtttatcttt ctttgccgga cagcgaaccc gattgttctt
     3001 cctacgatct tccggatttg ccgaccggat acggttatca ttgcgtggcc gaaacagatt
     3061 tgagaaaagt caacggttcg ggttggatac cggttgattt cacttccatc cccggaggat
     3121 ctcctttgtc cgttttaccc gtcgatccgg tgaacgattt tctccattat tacacttttc
     3181 tgaaaggagg atcgtacaaa ttgtcgacgg aaatggaatc aatcgcctat ggtccggccg
     3241 gcagcaatcc ggtgaccatc aaagacaacg gcataaacag ttttctttac gaagtcggca
     3301 cgaatctaac gcttgccgct ccggccaata tcctaataaa cagcgagatc gacaatctcg
     3361 tttccggtta tactcccggc tgggacacgg atttgaacgg aacttatcgg ccggccacgg
     3421 gttttagcgg gggttacaac agcggcgtcg cttctcccaa tatcggttat cacgcgcacg
     3481 cgaaacccga ttgcgggatc aacgaaagcg gctgtctcga atatatcgac gaaaattgcg
     3541 cttacgataa ttgccatcgc tggctcggag tgacccaaac ttggtccaac cccggcgttc
     3601 tcaaaggctg gacaaccggc acaaagatca gagttcgctt tctcgccaaa aagaacgtga
     3661 ccaacaagta tccccatttc gggttgcacc actattcgat ttccctcggg gccaatactt
     3721 ttgccggcgg tggcggaacc atcagcgcac cgttgccgca aatcggaaaa tggacagttg
     3781 tcaccggcga attcaccgtc actcccgatt gggagttaac aactcacggc gtcactttgt
     3841 atctttacgg ttacagcggc gctgatgaag gccggatatg gatcgacagt atagacgctg
     3901 tttattggaa tccgtaggat ttatctttat gggagtttta tattgttcta caagtttttg
     3961 atcgttgggg tttaacgata accgatccca caatcttctt ttttcgatga agcatgattg
     4021 aatacgcgag aaaccgtcga gcgttttgta taagcgctct ttataatcgg atttttcgct
     4081 taagatgttt ctctggagat atttgacgat tggaagagga ttgttagggc ctgattttaa
     4141 atacagattc cataatagac gattgccttt gtaggaatgg tagccaataa gttccttgaa
     4201 ccgttcgttg ttcatcaaag ctgcggtgag cgcttctttg aaataatata tggcttcttc
     4261 gtttagtttt ttcccggact ctttcttcca ttccgccaac tgctcgaaaa ataaaagatg
     4321 cgagatttca tgcaaagcaa tggcaaggat gctttgttcg ggagaatttt ctttcggaac
     4381 ggcataaaac cggaacgagt ttttatcaaa aggacaaaaa ggaagaaaag tagcgatggc
     4441 cgtataagtt tgtcgcttgg tcgggcgata gttcattaag gcatacaaaa taccaaacgc
     4501 tttgtccgat tttttaagca acaagcgatt actcgcgata tttctttcga tggcgcgtcg
     4561 gttttcagta taggcagttt caagatattt tcttactaag gctctaacct ttctttgcgg
     4621 ctgaccgata agcgatcgcg ccaaagcagg atgggcctta agaatcagct gtctatagga
     4681 aacgcgtttc gaatgcgtta aatattcggt aaaaatcgaa acatcggcct ccaagtcggt
     4741 ttcgattttt atgcttgggg cgatatttcg aggcatcgtt ttgtcgagat gaccctattc
     4801 tttatttttc cggaaggcct cgacgatctt gtccatgtcg ccttccatga ttttttcgat
     4861 attgtggaac gatttgttca ggcggtggtc ggttattcgg tcctgaggaa aattgtaagt
     4921 gcggattttt tccgagcggt cggccgtgcc gatctgctct cgccgcagtt cggaaacttc
     4981 gccgacagcc tctttttgtt tttgttcgta aagtttggcg gccaaagact ccatagccct
     5041 ttctttgttg gcgtattgac ttcgttccac ctgggaagaa acaacaatgc cggtcggtct
     5101 gtgggtgatg cggacggcgg tttccacttt gttgacgttt tggccgcccg ggccggacga
     5161 ccggaaaaaa acaacgtcga gatcgccggg atttatttgc acttttcccg gatcgacgat
     5221 cggcaaaacg gcgaccgagg cggtcgaagt gtgcactcgt ccggattttt ccgttttcgg
     5281 gattctttgg acacggtgca ccccggattc gtgtttgaaa atttcataaa catccgggcc
     5341 ttggatttgg gcgaccatgc tttttattcc atagagcgac gtgtcgttcg cgtcgatcgt
     5401 ctggaacttg aaaccatgtt tgacggcata ccgctggtac attctcgcca aatctccggc
     5461 aaaaatggaa gcttcgtctc cgccggcgcc ggctctgatt tcgagtaaaa ttttatccat
     5521 aataacgtca ataaattagc atattttaag gctttccgcc aagcgtgttt ttttggcgga
     5581 tatgaaaatc ccgtcttggc ggcgggattt cgaagtgtta tttcttggcg gttttcggtt
     5641 tggccgcggc tttggttttc ggcttggctt tgggtttgac ggttttttcc accaccgctt
     5701 tcttcaccct tatcttcttt acttttttgg gagtcgcctc ggctttggct tttttggcct
     5761 tgaatttttc cacgcgtccg gccgtgtcca aaagcttctc ctgaccggta tagaaaggat
     5821 ggcaggcgct gcagatttcg atctcggctt tttccagggt tccgcctatt ttccagactt
     5881 tgccgcaggc gcaggtgatc gccgcctcgg ggtagtattt cggatggatg tcttgcttca
     5941 taaaacccag aatatcagac aattatcggc ttgacaagcc tgttttcgac ctctattata
     6001 gtagcgcgtc ttttaagacg gctctttacc aatttagcga gtaaatacac atttataagt
     6061 aaaagatttt tttagttgtc ccaaatccgg atcagacagg tttcgtctaa aagattagac
     6121 tatgcggatt tccgcggaaa gcgcttccgc cgagatccgc gtaaaaaacc tgtaagatct
     6181 ctttaatgag agtttgatcc tggctcagga tgaacgctgg cggcgtggat aaggcatgca
     6241 a
//
LOCUS       DAOPQT010000012         6113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_14116_length_6113_cov_3.170353, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000012 DAOPQT010000000
VERSION     DAOPQT010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14116_length_6113_cov_3.170353"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKG74_00550"
     CDS             complement(<1..181)
                     /locus_tag="PKG74_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89808.1"
                     /translation="MPKLSQGQKEIILIAVNVMLLVVLLIGAFWGIGVLGGLLVQAFE
                     APTMVGGTGIFFNFEG"
     gene            complement(188..715)
                     /gene="pilO"
                     /locus_tag="PKG74_00555"
     CDS             complement(188..715)
                     /gene="pilO"
                     /locus_tag="PKG74_00555"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOB89809.1"
                     /translation="MLKDFRVKMAVVIGITLVAGSVLIFAVFQLKKSIAASSSEIKLM
                     RVDFLSRSRAITSLAELKEDAEEARVYTDVLNFSLPTKEDLPEFNSEVFDLERDNNLP
                     TSFKFSGSEIAPTPGTPGEIAFELNLTGSYDDIVNFLKDLEDSSYVTDIRNFDLVGGT
                     DDQYRAILQGVVFFH"
     gene            742..1548
                     /gene="dprA"
                     /locus_tag="PKG74_00560"
     CDS             742..1548
                     /gene="dprA"
                     /locus_tag="PKG74_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOB89810.1"
                     /translation="MIPKLLQNIPDPPAELFVKGSLSDDFKIAVVGTRKSTSEGRLIA
                     RTIAAELAKAKITIVSGLALGIDTASHEGCLEAKGKTIAVLGTGIDNIYPKENKPLAE
                     RILAKGGAIVSEYPGDTPGYKENFLRRNRIISGLCEAIVVVEAPIRSGSISTANFAAE
                     QGREVFVIPGPINHPNYAGSHKLIRDGARLVASAKDILEDLGIEVLDRAADLDDSEKT
                     IVEAVRVRALPIDKIMEITKLEPSKALPAIAGLVLKKIIRESDEGYVLNQ"
     gene            1581..3560
                     /gene="topA"
                     /locus_tag="PKG74_00565"
     CDS             1581..3560
                     /gene="topA"
                     /locus_tag="PKG74_00565"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOB89811.1"
                     /translation="MNLVIVESPTKAKTIGKFLGSKYKVESSFGHIRDLPKSKLGIDL
                     ERDFEPQYVIPTKNRKRVNELKKLSGKTEAVILATDEDREGEAIAWHLATALGLDPQK
                     TERIVFHEITDEAIKEALKQPRIIDMNLVNSQQARRVLDRLVGYKLSPFLWKKVMSHL
                     SAGRVQSVAVRLIAEREKEIRAFKSEAYYTIEALFEGNPNFSANLVRVNNEPIPTPGI
                     KDKAESEKIVSDLEKSDFRVEKIEEKTLKRSPLPPFTTSTLQQEASKRMRLSAKQTMM
                     IAQSLYENGHITYMRTDSVNLSKDSVTAAAAWLRANLGEKYAESSPRFFKTKSRMAQE
                     AHEAVRPTNPLLTPDQIKVEGREQKLYELIWRRFIASQMPAAVWNQGRIDIEAKSKND
                     SYLLASNGSVLKFDGYLKIWPSQFTEKELPKVKENEKLDLIEAKSAEHFTEPPPRYNE
                     ASLIKTLEEAGIGRPSTYAPIISVIQGRNYVTKNPDRRFEPTEIGELVNSVLTEHFPQ
                     IVDIDFTAKMENDLDEIAEGKIEWQKVVKNFYDPFAKTLEEKYESVEKQKPVETTDEV
                     CEKCGKPMVVKYGRFGKFLACSGYPECKTTKKIPGSDSVKTKEGLEIKCPECKEGQVI
                     RRRTKKGRFFYGCSRYPECKFASWTKPKSAEDENQ"
     gene            complement(3566..5065)
                     /locus_tag="PKG74_00570"
     CDS             complement(3566..5065)
                     /locus_tag="PKG74_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOB89812.1"
                     /translation="MVLDRFGKRGFALLEVPVKITQESFLLFRNRSRLQREPLTGIHT
                     GFTLVELLLVIGILGILTAATVVILNPAELLAQARDASRASDIRALKIGLQAYFIEHS
                     GSSMGTPGVSYISIPDSSAACANLSLPNGSYRCATSENYRKADSNGWIPLDFTAITTA
                     LSVLPVDPVNSGNSYYSYTVDGQGFTLSAHPESDKYAAESEGFVAVGGPTTKLTGGNF
                     PEGWVYVPRNSDFGTAGFWVMKYEAKCVAGTTPLTTPDSGSHTYNNTVQPCTGNYYIA
                     STSGGYPIANISHNNAKAYCEAIGAHLLTNDEYMTIATDASLVDSNWSDNSVGSDYLP
                     RGNSNALAAQDGSSLYGTGYNDFTHLRTLTLSNSSVIYDLAGNVHEHVQRSENNAGDL
                     MTAMTLPACSDGAAAIGYCDFVTASPYISAWSSAVVKEKVAPPNNTWYANHGMGRVYT
                     YKNGTSMGTTVFLRGGYWSTSTDAGAFSLSLVWGTAESASHTVGFRCSR"
     gene            complement(5150..5914)
                     /locus_tag="PKG74_00575"
     CDS             complement(5150..5914)
                     /locus_tag="PKG74_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOB89813.1"
                     /translation="MLARESLSLLLDGLDEGVILIDDRGRVVFMNDSAVRISCGEFSG
                     RSYREIFSGWTSASGSKGDAVYEIPPFMFSPGGGLIGIRCDKRPDDYYRLLLVCEDAA
                     CKDALTGIFNRHFLITEVEWLESLRRRQRRHHQTAFEVGVIFGDIDGLKKTNDIKGYA
                     AGDALIQRIADVFKKTLRESDLVFRYGGDEFVVLLPNQTREETEIVLHRLQSAAKGLP
                     VSLGIFFVDSEMPIDAGIEKAGEAMKKTKAARKSVL"
BASE COUNT         1668 a         1569 c         1530 g         1346 t
ORIGIN      
        1 atccttcgaa attgaagaaa atacccgtgc cgccgaccat agtcggcgct tcgaaagcct
       61 ggacgagcaa tccgccgaga acgccgatgc cccagaaagc accaatcagc aaaacaacga
      121 gcagcatcac gttgaccgcg atcagtatga tttctttttg gccttgggac agtttaggca
      181 tgtcgtttta atggaagaag accactccct gaagaatggc tcggtattgg tcgtcggttc
      241 cgccgaccag atcgaagttc ctgatatcgg taacataaga agagtcttcg aggtctttca
      301 gaaaattgac gatatcgtcg taggagccgg tcagattgag ctcgaaggcg atttctccag
      361 gagttcccgg agtcggagct atttcgctgc cgctgaattt gaagctggtc ggcaagttgt
      421 tgtctctttc gagatcaaaa acctccgagt tgaattcggg aagatcctcc ttggtcggaa
      481 ggctgaagtt cagaacatcg gtgtaaacac gcgcctcttc cgcgtcttct ttgagttcgg
      541 ccaaagacgt gatcgccctc gaacgcgaca aaaaatccac tctcatcagt ttgatttccg
      601 aactcgaagc ggcaatactc ttttttaatt ggaaaacggc aaaaatgagg acgctaccgg
      661 cgaccaaagt gatgccgatg acgacggcca ttttaaccct gaaatcctta agcatcacgt
      721 actattatat aataagtcaa agtgattccg aaactattgc aaaatattcc cgatcctccg
      781 gccgaattgt tcgtgaaagg aagtctttcc gacgatttca aaatcgccgt tgtcggcacg
      841 agaaaatcga cttccgaagg tcggctgatc gcgaggacca tcgccgccga actggcgaaa
      901 gcgaagatca cgattgtgag cggccttgcc ttgggcatcg acacggcgag ccacgaaggc
      961 tgcctcgaag ccaaaggcaa aaccatcgcc gttttgggaa cgggcatcga caacatctac
     1021 cccaaagaga ataaaccact ggcggaaagg attttggcaa aaggcggcgc catcgtttcc
     1081 gaatatcccg gagacactcc gggatacaaa gaaaatttct tgagaaggaa tcggatcatc
     1141 agcggacttt gcgaagccat tgttgtcgtc gaagcgccga taagatccgg cagcatttcg
     1201 acggccaact ttgcggctga acaaggccgc gaggttttcg tgattcccgg gccgataaat
     1261 cacccgaatt acgcgggatc gcacaaactg atccgcgacg gagcgaggct cgtcgcctcg
     1321 gccaaagaca ttctggaaga cctgggtatc gaggttcttg accgcgcggc ggatttggat
     1381 gattcggaaa aaacgatcgt cgaagctgtc cgcgttcgag cccttccgat tgacaagatc
     1441 atggaaatca ctaaacttga gccgtcaaag gcgctccccg cgattgccgg actcgtcctc
     1501 aagaaaataa tccgggaatc ggacgaaggc tatgttttga atcaatgagc gcctatggct
     1561 tacaataata aaatactcca atgaatctgg taatagtcga atcgccgacc aaggcgaaaa
     1621 ccatcggaaa attcttgggc tccaaataca aagtggagtc ttctttcggc catatccgcg
     1681 atctgcccaa aagcaaactc ggcatcgatc ttgaacgcga tttcgaaccg caatacgtca
     1741 ttccgacaaa gaatcggaag cgcgtcaacg aattgaaaaa actctccggt aaaaccgagg
     1801 ccgtcattct cgcgaccgat gaagaccgcg aaggagaagc catcgcttgg catctggcga
     1861 ccgcgctcgg ccttgacccg caaaagacag aaaggatagt tttccacgaa atcaccgatg
     1921 aagccatcaa agaggcgctc aagcagccac gcatcatcga catgaattta gttaacagcc
     1981 agcaggccag gcgcgtcctc gaccgactgg tcggctacaa gctttcgccg tttctctgga
     2041 aaaaagtgat gagtcatctt tccgccggcc gggtgcaatc ggtggccgtc cgactgatcg
     2101 ccgaacggga aaaagaaata agggctttca agtccgaagc ctattatacg atcgaagcgc
     2161 tttttgaagg caatccgaat ttttccgcga acctcgtcag ggtgaacaac gaaccgatac
     2221 caacgcccgg gataaaagac aaggccgaat ccgaaaaaat cgtttccgac cttgaaaagt
     2281 ccgatttccg cgtggaaaaa atcgaggaga aaaccctgaa gcgttctccg cttccgcctt
     2341 tcaccacgag cactcttcag caggaagcct cgaaaaggat gcggctttcg gccaaacaaa
     2401 caatgatgat cgcccaatcc ctttacgaaa acggccatat cacctatatg cgaaccgatt
     2461 cggtgaatct ctccaaggat tcggtgacgg cggccgccgc ttggctccgc gccaatttgg
     2521 gcgaaaaata cgccgaaagt tcaccgcggt ttttcaaaac caaatcaaga atggcccagg
     2581 aagcccacga agccgtgagg ccgaccaatc ccctgcttac acccgaccag atcaaagtcg
     2641 agggccgcga acaaaaactc tacgaattga tttggagaag attcatcgct tcccagatgc
     2701 cggcggccgt ctggaaccag ggccggatcg atatcgaagc caaatcgaaa aacgattctt
     2761 atcttctcgc ttctaacggc agtgttttga aattcgacgg ctatcttaaa atctggccga
     2821 gccagttcac cgaaaaagaa ttgccgaagg taaaagaaaa cgaaaagctc gacctcatcg
     2881 aagccaaatc cgcggagcat ttcaccgaac cgccgccgag atacaacgaa gcgagtctga
     2941 tcaaaactct ggaggaagcg ggtattggcc ggccgtcgac ctacgcgccg atcatctcgg
     3001 tcatccaggg aaggaactac gtgacgaaga accccgaccg ccgattcgag ccgacggaaa
     3061 tcggcgaatt ggttaactcc gtgctcacgg agcatttccc ccagatcgtc gacatcgatt
     3121 tcaccgccaa aatggaaaat gacctcgacg aaatcgccga gggaaaaatc gaatggcaga
     3181 aagtggtcaa gaatttttac gatccttttg cgaaaaccct cgaggaaaaa tacgaatccg
     3241 tggaaaaaca aaagcccgtg gaaacgaccg acgaggtttg cgaaaaatgc ggcaaaccca
     3301 tggtcgtcaa atacggccgc ttcggaaaat tcctcgcctg ttccggatat cccgaatgca
     3361 aaaccaccaa aaaaatcccc ggatcagatt cggtcaaaac caaagaagga ttggagatta
     3421 aatgtcccga atgcaaagaa ggccaggtca tccggcgccg caccaaaaaa ggcaggttct
     3481 tctacggctg cagccggtat ccggaatgca aattcgcctc ttggaccaag ccgaagtcag
     3541 cggaagacga gaatcagtaa acaaattacc ggctgcagcg gaatccaaca gtgtggctcg
     3601 cagattcagc cgtgccccaa actaaggata agctgaaggc ccccgcgtcc gtgctagtgc
     3661 tccaatagcc gccgcgcagg aagacggtgg ttcccatgct agtaccgttt ttgtaagtgt
     3721 aaacgcggcc cataccgtgg ttagcgtacc aggtattatt cggtggcgcg actttttctt
     3781 ttaccaccgc tgacgaccag gcggaaatat aagggctggc tgtcacaaaa tcacaatagc
     3841 ctatggccgc tgctccgtcc gaacaagctg gtaatgtcat ggcggtcatg agatctcccg
     3901 cattgttttc gcttctctga acatgttcat gtacgttacc ggctagatcg tagataaccg
     3961 aactgttcga cagagtaaga gttctaagat gagtgaagtc attgtaaccg gtaccgtaca
     4021 aacttgatcc gtcctgggcc gccaaagcat tggaattgcc gcggggtaga taatccgagc
     4081 cgaccgagtt gtccgaccaa ttcgaatcga ccaaagaggc gtctgttgca atcgtcatat
     4141 attcgtcgtt ggtcaataag tgggcgccga tagcctcgca ataagctttg gcgttattgt
     4201 ggctgatatt ggcgatcgga taaccacccg aggtagaggc gatgtaataa ttaccggtac
     4261 agggttgaac ggtattattg taagtatggg agccggaatc cggcgtggtt agaggcgtgg
     4321 ttccggcaac gcatttggct tcgtatttca tcacccagaa tccggcggtc ccaaagtccg
     4381 aattccgggg cacatagacc cagccttcgg ggaagttgcc gcctgtaagc ttagtggtcg
     4441 gtccgccgac tgcgacaaaa ccctccgact cggctgcgta tttatccgat tccggatgag
     4501 cggataaggt aaagccttgg ccgtcgaccg tgtagctgta atacgagttt ccagaattta
     4561 cgggatcaac aggcagaaca ctcaaggcgg tggtgattgc ggtaaaatct aaaggaatcc
     4621 agccgttcga gtctgctttg cgataattct ccgaagtggc gcagcggtaa gaaccgtttg
     4681 gtaaagataa gttcgcacaa gcggcgctcg aatccgggat ggagatatat gaaactccgg
     4741 gagttcccat ggagctcccg gagtgttcga tgaaataagc ttggagtccg atctttagag
     4801 cccggatatc gctcgctctc gaagcgtctc tggcttgagc caaaagttca gccgggttaa
     4861 ggataacgac cgtggctgcg gttaagattc cgagaattcc gatcacaagc aatagttcga
     4921 cgagggtaaa ccccgtatga attccggtta aaggctctct ttggagtcga gaccgattcc
     4981 taaacaataa gaaagattct tgggtgattt taaccggaac ttccaatagg gcaaaacctc
     5041 gttttccaaa acgatccaaa accatttatt cataatagac tataaaaaac accccgcaaa
     5101 atatgggggt gtaaaagcgc cgcggctttc gctgcggcgc ttgtgttgct caaagaactg
     5161 attttctggc ggctttggtt tttttcattg cctcgcccgc cttttctatt cctgcgtcga
     5221 tcggcatttc cgaatcgacg aagaagatcc cgagagagac gggtagacct ttggccgccg
     5281 attgaaggcg gtggagaact atctccgtct cctctctggt ttggttcgga agtagcacga
     5341 cgaattcgtc tccgccgtag cggaaaacga gatcgctttc gcgcagcgtc tttttgaaga
     5401 cgtcggcgat tctttggatc aaagcgtcgc cggcggcgta tcctttgata tcgttggtct
     5461 ttttgagacc atcgatgtct ccgaatataa ctcccacctc aaatgccgtc tggtgatgac
     5521 gacgttggcg gcgtcgcagc gactccaacc attctacttc ggttatcagg aagtgtctgt
     5581 taaagatgcc tgtcaaagcg tctttacagg cggcgtcttc gcagacaaga agcagacgat
     5641 aataatcgtc cggtctttta tcgcatcgga tgccgatcaa tcccccgccc ggagagaaca
     5701 taaagggcgg tatttcgtac accgcgtctc ccttactccc cgatgcggac gtccatccac
     5761 taaaaatttc tcgatacgaa cgtccggaga actcaccgca actaatcctg accgcggagt
     5821 cgttcataaa aacgactctg cctcgatcat cgatgagaat aactccttca tcgagaccgt
     5881 ctaatagaag agaaaggctt tctcttgcta acacgtttct tcctccttga tcgtatttcc
     5941 ggcactcctt taccggattt tgtgaagcta ctatatatta ttacaacata aattagctga
     6001 tttgtcaaag acattcggaa agaaatggac aaataaagct tttgaagttt aatataagcc
     6061 catggagcgg aaaagagatg tcaaaaaaca agctttggaa aaccgggcct ccc
//
LOCUS       DAOPQT010000013         6024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_14386_length_6024_cov_3.783213, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000013 DAOPQT010000000
VERSION     DAOPQT010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14386_length_6024_cov_3.783213"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="PKG74_00580"
     CDS             complement(<1..1508)
                     /locus_tag="PKG74_00580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOB89814.1"
                     /translation="MKQIFELLIDNPFGIMFIITGLSFLGIFLAVFWGWKYFRKALDK
                     INKWTIAVLIALFLLYLLFLIPGFKNGFIPSDEEWFGIKQAREIVDGDLQVFNHSWKG
                     IGYSFIVAGFMTVFGITEHAAIIANVFFSALTIILVGLTAYVASRSNVAAVSGAIGYA
                     SLPLVVKFAGFSMGYPAMGVFFMALFAFFGSVAVKEKSFASHVAMWLVAALASGIKPE
                     FIVLVFPAIVAGIMTIASTPRIKKKIKDNYPKLILFAIAITISFIPFLVKYYQADRNF
                     KDVNGTIGIYPSQKGDRPVSAIDKILRPFLNSRISFDYLAGDADNFLLFIVNLQKGTF
                     IAVIIIGILLYAIKRRNKKDATAKIFMFLIITACCVAAIYMAADVIFDEEGGRFAFYI
                     LPFVSIMFGSGLAAIFDYLQDKAPLYLAVIPLLILPAYIVNVVFFTVPEASRSYNHDL
                     VLVLNQGRIVVASNKIDKTKTTIYTKNGNLTNFFRFNGYNSYSFTDMVNTGTP"
     gene            complement(1505..3169)
                     /locus_tag="PKG74_00585"
     CDS             complement(1505..3169)
                     /locus_tag="PKG74_00585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOB89815.1"
                     /translation="MIFNNGTLIFSAAAIFIVAFASLSSLIPDKLGKRIKIDSWWKKA
                     GILLLIFVLLLVFSNILGQKTGFYPSDEEWEEAYFAKQLIDPSSEAIVGRMRHGFFFP
                     AVFVFGNTVFGKINGPATINAFFLISAVILTGILAAKLSGKTWPGIVASLLLALNPAW
                     LYGTAVFSGYPTLMSFCLSGFTLFAVLAVKEKKFIDYLGMITFAGMAFAAKIEYGVLF
                     AVVGIAILMGFDRKEYKKSLLVAAIALIFILVLTTTIVNMREGDQFCGSEAQVGLVSQ
                     KIEKAVGSSIIRPFERIVKKIGGWRFSIAYTLDDIPRVIDYWTRKELLPISLIVILGI
                     FFGIFKKDKGILIATVAFMATTLVYMTDCANYVQRFAIPNITIAAAIAATTIRNIPAE
                     IMIKRKKKKRTKVNEFDLGSIAVIIVGIMLLFYIPRAINNLAYRRIERPTPEDMWRVL
                     SHIPDTATIVMTSHHNEMMQLIFLENRNIKFVSLNDMFDRIDKSDGSNICSPEMNFCG
                     ENSYFIYTSSCDFFEHTKKICDTVSGFGEKIVSFDKIGVSIYRMVK"
     gene            complement(3275..3418)
                     /locus_tag="PKG74_00590"
     CDS             complement(3275..3418)
                     /locus_tag="PKG74_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89816.1"
                     /translation="MEYFEQVWNLIKSAGPLFVSLAQVIGRILVLTLDFITNLIRNGL
                     SRI"
     gene            complement(3483..4187)
                     /locus_tag="PKG74_00595"
     CDS             complement(3483..4187)
                     /locus_tag="PKG74_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOB89817.1"
                     /translation="MAREREIKIELHIPLETFIGRIKESGFEKEKEISQRDHYFDTLD
                     WRLYKTAAAFRIRQVNGKDHSFAFKKMFCLPKKHDRLYIEEIEGGLPVADRDKLDYIF
                     KRLGVDDKSLELSNGESLAKILSENGFQGEQILAKTRQIFKNGQEDEIVVDDVENVGT
                     IVELECQNDEPLDFAGTILKDDEWSRSVLGTSYIWLEKVKGFTDYLEYQTRFDKNPEW
                     NVWENEKEIYEHFLSE"
     gene            complement(4194..5432)
                     /locus_tag="PKG74_00600"
     CDS             complement(4194..5432)
                     /locus_tag="PKG74_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOB89818.1"
                     /translation="MKHLKTILILLALVFGYGLVYSANAATSSELQSQIDAKTRELQN
                     VQAEISSAQAQMAVISKQSQSLDKELKTTENTLNQLNLSIKSSTLNIQKLTLEIEDLN
                     SRIGGIGVDVEAKKKAIGLILAEIQKNDNEGTLQTFLKSMSLADSLLEMQSLTNLQEN
                     LSVEISSLNKLSNDLENNVRQTADKKRAIQTENVNLVNRKAIIEDQKDYQKELLAESK
                     NQEKIYQENLDRLAEKQAEIAAEREKIEQELKAKLDPSSVPASKSGILAKPCLGPIVQ
                     QYGRTAFALKAYKNQFHNGLDFDLNIGDPIFSAEDGKVVATGDLDLYCPRQSYGRFIV
                     ITHNNNLTTLYGHLSRIIVKSGDTVERGQLIGYAGNTGYSTGPHLHFTVYASSSFYMG
                     ASKSCRISPYGADLNPNNYL"
     gene            complement(5439..>6024)
                     /locus_tag="PKG74_00605"
     CDS             complement(5439..>6024)
                     /locus_tag="PKG74_00605"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89819.1"
                     /translation="GWHPQGNQEIFSREELINEFDISRVQKAGAVFDIEKLNWINRQY
                     IAGLSEEDLTAKIEKYIPEEWKINQAMVRAIKPRLDKIGDVKELLMVYFKLPEYDRNL
                     LFWKGEDRDTKNILEKLSAEIKNIEDKEFDLENVTDKITALVPDDKKGDYLWPLRVSL
                     SGSKTSPTPFELLSGLGKEESLRRIQIAVGKLNG"
BASE COUNT         1604 a         1344 c         1329 g         1747 t
ORIGIN      
        1 ggggtgccgg tattaaccat atcggtaaaa gaatagctat tataaccgtt aaatctgaag
       61 aaattagtca gattgccgtt tttcgtataa atcgtggttt tggttttatc gattttattg
      121 cttgccacga caatccttcc ttggttgagt actaatacta aatcgtgatt gtaagagcgc
      181 gatgcctcag ggacagtaaa aaacacaaca ttcacaatat aagccggaag tataagaagc
      241 ggaataacgg ctaaataaag cggcgccttg tcttgaagat agtcgaaaat cgccgcaagc
      301 ccggagccaa acataatgga aacgaaaggg aggatgtaaa aagcgaacct tcctccctct
      361 tcgtcgaaaa taacatcagc cgccatataa atggccgcaa cgcagcaagc ggtaataatc
      421 aaaaacataa atattttcgc ggttgcgtct tttttgtttc ttcgttttat cgcataaagc
      481 aatatgccga ttatgattac ggcgataaat gttccctttt gcaggttgac gataaataac
      541 aagaaattat cagcgtctcc cgccaaataa tcaaaagata tcctgctgtt caaaaaaggt
      601 cttaatattt tatctatggc ggaaacgggc cggtctcctt tttgcgacgg gtatattcct
      661 attgtcccgt tcacatcttt gaagtttctg tcggcttggt aatatttgac taagaacgga
      721 ataaaagaaa ttgtaatggc gatggcgaaa agtatcagtt tagggtagtt gtcttttatt
      781 tttttcttga tccgaggcgt ggatgcgatc gtcatgatgc cggcgacaat agccggaaac
      841 acaagaacga taaattctgg cttgattccg gaagcgaggg ccgcgacgag ccacatggca
      901 acatggcttg caaacgattt ttcttttacg gccacgctgc cgaaaaaagc gaaaagagcc
      961 ataaaaaaca cccccattgc cggatagccc atactgaaac cggcgaattt aacaaccaac
     1021 ggcaaagaag catacccgat ggcgccagaa acagcggcca cgttgctcct ggaggcgaca
     1081 taagcggtca gtccgaccaa aattatcgtc aaagccgaga aaaaaacatt ggcgataatg
     1141 gccgcgtgtt cggtgattcc gaaaacagtc ataaatccgg caacgataaa agaatatccg
     1201 atgcctttcc aggaatgatt gaaaacttgt agatcgccat caacaatctc tctcgcctgc
     1261 ttgatgccga accattcttc atccgacggg ataaatccat ttttgaatcc gggaatcaaa
     1321 aacaataaat aaagaagaaa gagtgcgatc aacaccgcta tggtccactt gtttatttta
     1381 tcgagtgctt ttcggaagta tttccatccc caaaatacag ctaaaaatat tcccaagaaa
     1441 gacaatccgg tgatgataaa cattatcccg aaaggattgt ctatcaataa ttcgaagatt
     1501 tgcttcattt gaccattctg tatatcgaga ctcctatttt atcgaaagag actatttttt
     1561 cgccgaaacc gctgacggtg tcgcagattt ttttggtatg ttcgaagaaa tcgcaacttg
     1621 aggtgtatat aaaatacgag ttttctccgc aaaaattcat ttccggagaa caaatgttcg
     1681 atccgtcgga tttgtcgatt ctgtcaaaca tgtcgttcaa ggaaacaaat tttatgtttc
     1741 tgttttcgag aaagatcaat tgcatcattt cgttgtggtg gcttgtcata acgatagttg
     1801 ccgtgtccgg aatatgagat aaaactcgcc acatgtcttc aggggtcggt ctttcgattc
     1861 tccgataagc taagttgttt atcgccctcg gaatataaaa gagaagcatg attccgacga
     1921 tgatcacggc aatactccca agatcgaact cgttcacttt cgttcttttt ttcttctttc
     1981 ttttgatcat tatttccgcg gggatattgc ggatggttgt tgccgcgatc gccgcggcaa
     2041 tcgtgatatt gggaatggca aagcgctgca cgtaattggc gcagtcagtc atataaacca
     2101 gtgttgtcgc cataaaggcg acagtcgcaa taaggattcc tttgtctttt ttgaagatgc
     2161 cgaaaaatat tcccaaaata acgatcaaag aaatcggaag aagttctttt cgcgtccaat
     2221 aatcgatgac tcgcggaatg tcgtccagag tataggcgat cgagaatctc catcctccta
     2281 tttttttcac gatccgttcg aaaggcctaa taatcgaaga gccaaccgct ttctctatct
     2341 tttgactcac caatccgact tgggcttcgg agccgcagaa ttggtcgcct tctctcatat
     2401 ttacaatggt tgtcgtgagg acaagtataa aaatcaaagc tatagccgcg accaataagg
     2461 attttttata ttcttttcta tcgaatccca ttaaaatcgc gatcccgact acggcgaaga
     2521 gaacgccgta ttctattttg gccgcaaagg ccattccggc aaaggtaatc atcccgagat
     2581 aatcaataaa ctttttctcc ttgacggcca aaaccgcgaa tagcgtgaac ccggaaagac
     2641 aaaacgacat caaggtcgga tatccggaaa ataccgccgt gccgtaaagc caggccgggt
     2701 ttaaagccaa aagcagtgag gcgacgatac ccggccatgt tttaccggat aatttggccg
     2761 caagaattcc cgtaagaata acggccgata tcagaaaaaa agcgtttatg gtggccgggc
     2821 cgtttatttt tccgaaaaca gtatttccaa acacgaatac cgccgggaag aagaatccat
     2881 gacgcatacg gccaacaata gcttcactgg acggatcgat taattgcttc gcgaagtaag
     2941 cttcttccca ctcttcatcg ctcggataaa atccggtttt ctgccccagg atattggaga
     3001 agacaagtaa gagaacgaat atgagtaata agatccccgc ttttttccac caagaatcga
     3061 tctttatccg ttttcccaat ttatcgggaa taagggacga gagcgacgcg aaagcgacaa
     3121 taaaaatagc ggcagcggaa aaaataaggg ttccgttgtt gaatatcatt gaataaacaa
     3181 attatacact attcttttga agaaagactg cgttgataaa taaaaaacga ctctttcgag
     3241 tcttaagctt tataattcgg tctcggactt tctgttaaat ccggctcaat ccgttacgga
     3301 taagattggt gatgaaatcc aaggtgagca ccagtattct gccgatcacc tgcgccagcg
     3361 agacgaatag cggtccggcg gattttatga ggttccaaac ttgttcgaag tattccattt
     3421 atccaatcat agcatttctt acgttatcca agaaatcctc ctgttttaac aaatatcgcg
     3481 tttcattcag aaagaaaatg ctcgtatatt tctttttcgt tttcccagac attccactcc
     3541 ggatttttgt cgaatcgcgt ctgatactcc agataatcgg tgaatccttt aactttctcg
     3601 agccagatat aactggtgcc caaaacgctt ctcgaccatt catcgtcttt caagatcgtt
     3661 ccggcaaaat ccagcggttc gtcgttttga cattccaatt cgacgatggt gccgacattt
     3721 tccacatcat cgacaacgat ctcgtcttct tgtccgttct tgaatatttg tctggttttc
     3781 gcgaggattt gttctccctg gaatccgttt tccgaaagga ttttcgcaag actctctccg
     3841 ttcgataact ccagactctt atcgtccacg ccaagtcgtt tgaaaatata atcgagtttg
     3901 tcgcgatccg cgaccggcag acctccttct atctcctcga tatacaaacg atcgtgtttt
     3961 tttggcaggc agaacatttt cttgaaagcg aaagaatgat ctttgccgtt aacttgcctt
     4021 atccgaaacg cggcggcggt tttatacagt ctccaatcga gggtatcaaa ataatggtcg
     4081 cgttgggaaa tctctttttc tttctcgaaa ccggattctt ttattcttcc gataaacgtc
     4141 tcaagcggaa tatggagttc tatcttgatt tccctttctc ttgccatcgg cgtttagaga
     4201 taattgttgg gattcaagtc cgcgccgtag ggagaaatgc ggcaggattt gctcgcaccc
     4261 atgtagaaac tgctcgaagc gtaaacggta aagtggagat ggggcccggt cgaatacccg
     4321 gtgttgccgg cgtagccgat caactgtccc ctttccaccg tatctccgga tttgacgatg
     4381 atgcgagata gatgtccgta aagcgtggtc agattgttat tgtgggtgat gacgataaag
     4441 cgtccgtagg attgccgcgg gcaatagaga tccaaatctc cggtcgcgac cactttcccg
     4501 tcttcggccg agaaaatcgg atcgccgata ttcaaatcga aatcgagccc gttgtggaac
     4561 tggtttttgt aagctttcag ggcaaaagcg gtgcggccgt attgctggac gatcggcccg
     4621 aggcaaggct tcgccaaaat accggatttc gaagccggta cggatgacgg atccagcttg
     4681 gctttcaatt cctgttcgat cttttccctt tcagccgcga tttcggcttg tttctccgcg
     4741 agcctgtcga gattctcttg gtagattttt tcctggtttt tggattcggc aagaagttct
     4801 ttttggtaat ctttttggtc ttcaatgatg gcttttctgt tgaccaagtt gacgttttcc
     4861 gtctgaatgg cgcgtttttt atcggccgtc tgtctaacgt tgttttcgag atcgttcgac
     4921 agcttgttta aagaagatat ctccaccgaa agattctctt gtagattcgt caagctttgc
     4981 atttcgagca ggctgtcggc gaggctcatg gacttcaaaa aggtctggag agttccttcg
     5041 ttgtcgttct tttggatttc ggccaaaatg agcccgatag cctttttctt ggcttcgaca
     5101 tcgacgccga ttccgccgat ccggctgttc aaatcctcta tttccaaggt gagtttctgg
     5161 atattcaggg tgctcgattt gatgctcaaa ttgagctggt tcaaggtatt ttccgtggtt
     5221 ttcaattcct tgtccaagct ttggctttgt ttggaaataa cggccatctg cgcttgggcg
     5281 ctgctgattt cggcctgaac gttttggagt tctctggttt tggcatcgat ttgggattga
     5341 agctcggacg aagtcgcggc gttcgcggaa tatactaaac cgtaaccaaa aaccaaagcg
     5401 agtaaaatca gaattgtttt aaggtgtttc atgagagatt atccgttcaa ttttccgacg
     5461 gcgatttgga ttcttctcaa agattcctct tttccgaggc cggacagaag ctcgaacggc
     5521 gtggggctgg ttttggagcc ggaaaggctg acgcgaagcg gccaaagata atcccctttt
     5581 ttgtcgtccg gcaccaaagc cgttatttta tccgtaacgt tttccaaatc gaattctttg
     5641 tcttcgatgt ttttaatctc ggccgacagt ttttcgagaa tatttttggt gtcgcggtct
     5701 tctcctttcc agaaaagcag atttctatca tattcgggca acttgaaata aaccatcaaa
     5761 agctctttga cgtctccgat tttatccaaa cgcggtttga tcgcccggac catggcctga
     5821 tttattttcc attcttcggg aatatatttt tcgattttag cggtaagatc ttcttccgaa
     5881 aggccggcga tatattgacg gttgatccaa ttaagttttt cgatatcgaa cacggctccg
     5941 gctttctgca cccggctgat atcgaattcg tttatcaatt cttcgcggga aaagatctcc
     6001 tggtttccct ggggatgcca accg
//
LOCUS       DAOPQT010000014         5938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_14651_length_5938_cov_4.074792, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000014 DAOPQT010000000
VERSION     DAOPQT010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14651_length_5938_cov_4.074792"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PKG74_00610"
     CDS             complement(<1..868)
                     /locus_tag="PKG74_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89820.1"
                     /translation="MSASEKIGQILRTPVGVLEKADQGLIAVSGKTDILERIVEENEK
                     RIRTRLDALGLGRNVYASEVYDSLISRIEFDDNKLFEALGSPSFKSSGDWQKVLDAAK
                     QAAGSPKGFFIKEEKAKELILSEPPQNIMNLLGYGSASEMLGKENLFEVFAALRFVEG
                     NEWLNNVFFKQYESLIASDFEERPVEAIALSDRWLKASEGFIKKKYHNISHLKELGVI
                     FSLPLHLVISGELLRNFSLILHYFNEVDFYSDIFRGYKDDVDFAKKIIVLLKGESDEP
                     RPAFNGQNWLILP"
     gene            complement(872..1861)
                     /locus_tag="PKG74_00615"
     CDS             complement(872..1861)
                     /locus_tag="PKG74_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOB89821.1"
                     /translation="MFDVISIGSAVYDGFMETDLKEIKDKTTAFGKSLRLPLGEKISG
                     RNVSFEIGGNAVNAAITFSRQGYRTGIFARIGRDIQGEEIRRRLAEEKIKLDFMEFDS
                     RIQTSFSAIFLSKGERTIINFPGSGNNLSLAGLLKAKPKTKWFYVSLPGDSYKALPNI
                     VSLAKKQSAKIAFNPTMRHIRESKQTILKLLPKMDFLVLNETEASILTGIDFENPIKS
                     FRKLDKAMPGILAVTYGQKGAAVSDGRFVYRTGIFQNRKTVDRTGAGDAFGSGFVSGL
                     MRGDIKLAIRLGLANAASVVEAIGANKNSLTRNDFEKSNRWSKVSIKTEKINP"
     gene            1956..2207
                     /locus_tag="PKG74_00620"
     CDS             1956..2207
                     /locus_tag="PKG74_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89822.1"
                     /translation="MKNSWGIIVILAVFMGMFYLYDSLHPQSIVTPENQEIEEAALVG
                     NANQGLPTPEEPAKPDLDLESLSGLEAELNPLEELRETL"
     gene            2233..2772
                     /locus_tag="PKG74_00625"
     CDS             2233..2772
                     /locus_tag="PKG74_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOB89823.1"
                     /translation="MLEDERNLIIRAKGGEAEAFGLLYDEYLPKIYRFVLLKTGHREE
                     AEDLTHQVFLQAWRNIRNYDDRGYPFSSWLYRIAKNLVVDYYRRLRPNVSLEDCRVFP
                     ADEVDPETDLDLSRETEKLARAVGKLRDTEQTVVIMRFVDELSIKETAAAIEKSEGAV
                     KLIQHRAIQKLKKLMTEEK"
     gene            2776..3228
                     /locus_tag="PKG74_00630"
     CDS             2776..3228
                     /locus_tag="PKG74_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89824.1"
                     /translation="MKQIESQLKNLRKIRPDKGFALMCREDLLSSTVVKPGIDLWRNL
                     GLSLSLALTAMLVVFVAQNAAVSSYSIASLEKFEEETTLSRDQINITLAEINYHSDLA
                     SKTSMALSEAAAEGPSHLNQNLLMKEAESLKIDSAPDNVDILLDQVIL"
     gene            3230..4105
                     /locus_tag="PKG74_00635"
     CDS             3230..4105
                     /locus_tag="PKG74_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89825.1"
                     /translation="MKKTNFLIASGLVSLIVFFPKPVFGAEIKANAENLTTSVNQLIE
                     VKDDKSMSDPERLAKELEARKQVVKDALSLSLKEISSLETSLKALKTKDERVLDFQSS
                     SLEYLESAKTFYEEQSDVVDKLASMDEVKALAQKIKDYRDAGYNKKIERVTNFLLLNQ
                     IDGLIATAETRWQKIDSDLKKLERGAFVSPGYFDANMQKAKKYISDATNLGADAKEQI
                     LKEFEAEKISAEEKLSLTAASPSEDKAQVQDNTPEIAPKVVNPKELAEASLTNLKSSY
                     DIFLKISQSVKKILQ"
     gene            complement(4141..4416)
                     /locus_tag="PKG74_00640"
     CDS             complement(4141..4416)
                     /locus_tag="PKG74_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003659331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOB89826.1"
                     /translation="MKKSGLVEAVMKAANIDTKKQAEMAVEAVFDTIVKTMSRGEEVA
                     ITGFGTFRVAKRAARMGINPKTGEKIHIAASVKPKFRAGKMLKEAVL"
     gene            complement(4504..>5938)
                     /gene="rny"
                     /locus_tag="PKG74_00645"
     CDS             complement(4504..>5938)
                     /gene="rny"
                     /locus_tag="PKG74_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOB89827.1"
                     /translation="IPLDAVQAVVFPEFRKRLDDALQRLVAQDEPYDEEFKIRNAATG
                     KTCYVHSKAIRLTDEEGNAVKVVGAIQDITTYKKSEEEIATEKSEVDNLREDLKKELV
                     KLSGLTPQEAKEKLIKEVEDQHRSELKDLLQKFEKEKRDEVEKKSLEVITTSIQRYAR
                     SHVSDITTTSYTLPNEDLKGKIIGREGRNIRTLERLTGVELIIDEAPDTIIISSFDPM
                     RREICRMALEKLIKDGRIQPAKIEEKVEEARLELDKRMYEIGEAAAYEAGVVDLPKEI
                     IQLLGRLNFRTSFGQNVLSHSIEVAAIAGMIAEELGINADVTRKAALLHDIGKAIDHE
                     VAGNHVELGIKILKKYSVDDRVITAMQSHHEDYPFSSPESFIVATADIVSAARPGARR
                     GTLENYLKRLEDLEKIASTFEGVKNSYAISAGRELRVFVVPEKIDDFGALQLAKNIAA
                     KIEAELKYPGDIKVNIIREVRAVEYAR"
BASE COUNT         1439 a         1527 c         1446 g         1526 t
ORIGIN      
        1 gcggcagaat aagccaattt tggccgttaa aagccggacg cggttcgtcg ctttcgcctt
       61 tcaaaagaac gattatcttc ttggcgaaat cgacgtcgtc tttgtagcct ctgaaaatat
      121 ccgagtagaa atcgacttcg ttaaaatagt gaaggatcaa gctgaaattc cgcaaaagct
      181 ctccggagat cacgaggtgt aggggcaggc tgaagatcac tccgagttct ttcaaatggc
      241 tgatgttgtg atatttcttc ttgataaatc cctcgctcgc cttgagccag cggtcggaca
      301 aggcgatcgc ctcgaccgga cgctcttcga aatccgaagc gatcagagac tcgtattgtt
      361 tgaagaaaac gttattcagc cactcgtttc cttcgacgaa acgcaaagcc gcgaaaacct
      421 cgaaaagatt ctctttgccg agcatctccg aagccgagcc gtagccgagg agattcatta
      481 tgttttgcgg cggctcgctc aaaatgagtt ctttggcctt ttcttctttg atgaaaaatc
      541 ctttgggcga tccggccgct tgtttggccg cgtcgagcac tttttgccaa tcgccggacg
      601 acttgaacga aggacttccc aaagcctcga aaagtttgtt gtcgtcgaac tcgatacggc
      661 tgataaggct gtcgtagacc tcgctcgcgt aaacgttcct tccgaggccc aaggcgtcga
      721 gcctggttct gatccttttt tcgttttctt cgacgatcct ctcgagaata tcggttttgc
      781 cggaaaccgc gatcaagccc tggtcggctt tttcaagaac accgaccggc gttcgcaata
      841 tttgaccgat tttttccgaa gcggacatga tttatggatt gatcttttcc gtttttatcg
      901 agacctttga ccaacggttg gacttctcga aatcatttct cgtgaggcta tttttgttcg
      961 cgccaatcgc ctcgacaacc gaagcggcgt tcgcgagccc cagcctgatg gcaagcttga
     1021 tatcgcccct catcaaaccg gagacaaaac ccgatccgaa agcgtctccg gcgccggtac
     1081 gatccacggt tttcctgttt tggaaaattc ccgtccggta gacgaatcgg ccgtcggaaa
     1141 cagccgcgcc tttttggccg taagtgacgg cgaggattcc gggcatggct ttgtcgagtt
     1201 tgcggaaaga tttgatcgga ttctcgaaat cgattccggt caagatcgag gcttcggttt
     1261 cgttcaaaac cagaaagtcc attttcggca aaagcttcaa aatggtttgc ttggattccc
     1321 ggatatggcg catggtcgga ttaaaagcga ttttggccga ctgttttttc gccagagaga
     1381 cgatgtttgg caacgcctta taggaatctc cgggtaaaga tacgtaaaac catttggttt
     1441 tgggttttgc cttcaaaagc ccggcaagcg acaagttgtt gccgcttccc ggaaaattga
     1501 tgatcgtccg ttctccttta gaaaggaaaa tggccgagaa actggtttgg atccgggagt
     1561 cgaattccat aaaatcgagt tttatttttt cttcggccag ccggcggcgg atttcctctc
     1621 cctgaatatc ccggccgatt ctcgcaaaga ttcccgtgcg atatccctgt cggctgaaag
     1681 tgatggcggc gtttacggcg ttgccgccga tctcgaaaga aacatttctt ccggatattt
     1741 tttcgccgag aggcagccta agcgatttcc cgaaagcggt cgttttatcc ttgatctctt
     1801 tcaagtccgt ttccataaag ccgtcgtata cggccgaacc gatactgatc acgtcgaaca
     1861 tggtttaagt atatcgaaaa aaccggtttc caaaaagaaa ttctccggag cgcagccgtc
     1921 ttccttagct gccgaaaatc tgttaagatc aagttatgaa aaactcttgg ggcatcattg
     1981 ttatcttggc cgtgttcatg ggaatgttct atctttacga ctcgctccat ccacaatcga
     2041 tcgtgacccc ggaaaatcag gaaatagaag aggccgccct tgtcggaaac gcgaaccaag
     2101 ggcttccgac tcccgaagaa ccggcaaagc cggaccttga tctggaatct ttaagcggtt
     2161 tggaagcgga gcttaacccc cttgaagaac tccgggaaac cttgtaactc ttttccagtc
     2221 taaacgtttc ctatgttaga ggacgagcga aatttaatta tccgtgcaaa aggtggggag
     2281 gccgaggctt ttggcctgct gtacgacgaa tacttgccga agatatatcg tttcgtcctg
     2341 ctgaaaacag gtcatcgtga ggaagccgaa gaccttaccc accaagtttt tttacaagcc
     2401 tggagaaata tccggaatta cgacgaccgg ggttatccct tttcgagctg gctttatcgc
     2461 atagccaaaa acctcgttgt cgattattat cgccgcttgc ggccgaacgt ttctctcgaa
     2521 gactgccggg tcttccccgc cgacgaagtt gatccggaaa ccgatctcga tctttcgcgg
     2581 gaaacggaaa aactcgcccg ggccgtcgga aagctcagag atacggaaca aacagtcgtc
     2641 attatgaggt tcgtggacga actttccatc aaagaaaccg cggccgccat cgaaaaaagc
     2701 gaaggagccg tcaaattgat ccaacatcga gccattcaaa aactgaaaaa actgatgacc
     2761 gaagaaaaat aagaaatgaa gcaaatagaa tcccaattga agaatttgcg taaaatccgg
     2821 ccagacaaag gtttcgcttt gatgtgccgg gaggatctgc tctcttcgac ggttgttaaa
     2881 cccggaatcg acctttggag aaatctcggt ttgagccttt ctcttgccct gaccgccatg
     2941 ctcgtcgtat tcgtggccca aaacgcggct gtttcttcct attccatcgc cagcctggaa
     3001 aaattcgaag aagaaaccac cttgtcccgg gatcagataa acatcaccct ggccgagatc
     3061 aattaccatt cggacctggc ttcgaaaacc tcgatggccc tttccgaagc cgcggccgaa
     3121 ggcccgtccc atttgaatca aaaccttctg atgaaagaag ccgaaagtct gaaaatagat
     3181 tccgctccgg acaacgtcga tattcttttg gaccaagtga ttttataaaa tgaagaaaac
     3241 aaacttttta atcgccagtg gcctcgtctc tcttatcgtg tttttcccga agccggtttt
     3301 tggagccgaa atcaaggcta acgccgagaa tttgacaacg agcgtcaatc agctgatcga
     3361 ggtgaaagac gacaaatcga tgagcgatcc tgaacgtcta gccaaagaac tggaagccag
     3421 aaaacaagtg gtcaaagacg ccctgtcgct ttctcttaag gaaatctcga gcctggagac
     3481 atcactgaag gccctgaaaa ccaaagacga acgcgttctc gatttccaaa gctcttcgct
     3541 cgagtatctc gaatcagcta aaacctttta cgaagaacag tccgacgtcg tcgataaact
     3601 cgcgagcatg gacgaagtga aagctttggc ccagaaaata aaagattatc gggatgcggg
     3661 ctacaacaag aaaatcgagc gcgttaccaa tttccttctt ctgaatcaga tcgacggcct
     3721 gatcgccacg gccgaaaccc gctggcagaa aatcgattcc gatctgaaaa agctcgaacg
     3781 gggagctttt gtcagtcccg gatatttcga cgccaatatg cagaaagcga aaaaatatat
     3841 cagcgacgcg acaaatctcg gagccgacgc caaagagcaa atcttgaaag aattcgaagc
     3901 cgaaaaaata tcggccgagg aaaaattatc tttgacagcg gcttcgcctt cggaagacaa
     3961 agcccaagtt caagacaata cccctgagat tgcgccgaaa gtggtgaatc cgaaagaact
     4021 ggccgaagcc agcctcacca atctgaaatc gagctacgac atttttctca aaatcagcca
     4081 gagcgtcaaa aaaatcctcc agtaatccgg aggatttttt cggaataagt atggaagcgt
     4141 ttacaaaacg gcttctttca acattttgcc ggcgcggaat ttcggtttga ccgaagccgc
     4201 gatatggatc ttttctccgg ttttcggatt tattcccatt cgcgctgcgc gtttggcgac
     4261 tctgaaagtg ccaaatccgg tgatagccac ttcttcgccg cggctcatgg tctttacgat
     4321 ggtatcgaaa acagcttcca ccgccatctc ggcctgtttt ttcgtatcaa tgttggcggc
     4381 tttcatcact gcctcgacaa gtcctgattt tttcatgaat aagttgggct ttttatcaat
     4441 cgaccttttc cgatttatta agccaataat agcaaaccaa gggcaaaaag ccaagccata
     4501 gagctatctc gcgtattcca cggccctgac ttcccggata atgttcactt tgatgtcgcc
     4561 cgggtattta agctcggcct cgattttcgc cgcaatgttt ttggcgagtt gtaaagcccc
     4621 gaaatcgtcg attttctccg gtacgacgaa cacccggagc tcccggccgg cggaaatggc
     4681 gtaagaattc tttactccct caaaggtgga agcgatcttt tcgaggtctt ccagtctttt
     4741 caggtaattc tcgagggtcc cccttctcgc cccgggacga gccgccgaga caatatcggc
     4801 cgtggccacg atgaacgatt ccggggaaga aaacgggtaa tcctcgtggt gggattgcat
     4861 ggccgtgata acgcggtcgt cgacgctgta ttttttcagg atcttgatgc cgagttcgac
     4921 gtggttgccg gcgacctcgt gatcgatcgc cttaccgatg tcgtggagca aagccgcctt
     4981 tctcgtgacg tcggcgttga tccccaattc ttcggcgatc atgccggcga tggccgccac
     5041 ctcgatcgaa tgggaaagca cgttctggcc aaagctcgtc cggaaattaa gtcttcccaa
     5101 aagctggatg atttctttcg gcaaatcgac gacaccggct tcgtaagcgg ccgcttcgcc
     5161 gatctcgtac atccttttgt cgagttcgag tctcgcttcc tcgactttct cctcgatctt
     5221 ggccggctgg atgcgtccat ctttgatcag tttttccaaa gccatgcggc aaatttcgcg
     5281 ccgcatcgga tcgaacgaag aaataatgat cgtatccggc gcttcgtcga tgataagctc
     5341 gacgccggtc aatctttcga gtgtcctgat gttccggccc tcgcggccga tgatcttgcc
     5401 tttcaaatct tcgttcggaa gagtgtagga agtggtggtg atatcggaca cgtgcgatcg
     5461 ggcgtaacgc tggatggaag tggtgatcac ctccaaactc tttttctcga cctcgtcgcg
     5521 tttttctttt tcgaacttct gaagcaaatc cttgagctcg ctccggtgct ggtcttccac
     5581 ttccttgata agcttttcct tggcttcctg gggagtgagg ccgctcaact tgaccagctc
     5641 ttttttgagg tcttcccgaa gattgtcgac ttcggatttt tccgtggcga tctcttcttc
     5701 ggatttttta tacgtcgtta tatcctggat cgcgccgacc accttgaccg cattgccttc
     5761 ttcgtccgtg agccggatcg ccttggagtg gacgtagcag gttttcccgg tcgccgcgtt
     5821 gcggatcttg aattcttcgt catagggttc gtcctgcgcg acgagtcttt gcagcgcgtc
     5881 gtccaggcgt ttgcggaatt ccgggaaaac gacggcctgc accgcgtcca gcggaatc
//
LOCUS       DAOPQT010000015         5907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_14747_length_5907_cov_3.766576, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000015 DAOPQT010000000
VERSION     DAOPQT010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14747_length_5907_cov_3.766576"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKG74_00650"
     CDS             <1..509
                     /locus_tag="PKG74_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOB89828.1"
                     /translation="GLAGRFWLLDYVVVFSAKWLIWLALAAFAIAVWRSKSLRTRAYF
                     FSLAALGAILSRGIFAEIVRYARYNPRPFEELGIDPLFGQYATASFPSGHVAFLFPIA
                     LAAWKYNKKLGIWLIAATILMGISRVIAGVHWMTDIAAGFLVGAIGFLVAQALLPRLE
                     KKGEEKLS"
     gene            540..1235
                     /locus_tag="PKG74_00655"
     CDS             540..1235
                     /locus_tag="PKG74_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOB89829.1"
                     /translation="MNEKTKIFLVPGAIVVAGIIIAMAVLYSGGVIDTTSQTAQVGEG
                     FAALKSLKAVNSDDHILGNASAKVKIVTFSDMDCPYCQRFNSTIKEILDIYGDKIAVV
                     YRHFPLTMLHPDAEKKSEATECAAKLGGNGGFWSYFDKYEEEAAAGKTFSEELLLDVA
                     ASIGLDREDFSTCLRSDEPGKLVKEDADNAAEIGITGTPFSIILSNNKPVDTIEGAYP
                     IEAVREKLDQYIK"
     gene            1322..1402
                     /locus_tag="PKG74_00660"
     tRNA            1322..1402
                     /locus_tag="PKG74_00660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1355..1357,aa:Leu,seq:caa)
     gene            1502..2140
                     /locus_tag="PKG74_00665"
     CDS             1502..2140
                     /locus_tag="PKG74_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89830.1"
                     /translation="MYPENRKGSVEVTKEKEKKAGGLSARELLEAVYNLLKKDGREIK
                     LPSKAMAEIANDSDGHRTRIGYTGYESAAILKVADREWILAFGTKCGQYPADPYNCDI
                     VAMRANLDGKTDKEAEPEIHQALERNTCFHHSLAYAMADGTLALNQNGCFCDKVIELL
                     RPKIGEFIARDLEVDPNCFTMDLRPVVKSAVQYKPEFIDFLRDTFLAVLASA"
     gene            2300..3103
                     /locus_tag="PKG74_00670"
     CDS             2300..3103
                     /locus_tag="PKG74_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOB89831.1"
                     /translation="MARVIAICNQKGGVGKTTTAVNLGSYLAAFGRKVLLIDFDPQAN
                     ASSALGYSSNDGGTIYQGLIGEAEPQKLIRPTMILNCHFIPSASHLSGALVEFVNLPE
                     REYFLKKFIDKVRNAYDYILIDLPPTLSLLMLNGLLASGEVIIPVQAEYYGLEGLSQL
                     LSTLELVRSNLNHDLKVAGALITMYDKWEHLSQEVAKNIRRNFPYYVFGVEIPRSVSL
                     AEAPSFGKPILLYAPYSQGALAYRRLASEVMQQENPITAETKEQTGNNM"
     gene            3105..4298
                     /locus_tag="PKG74_00675"
     CDS             3105..4298
                     /locus_tag="PKG74_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOB89832.1"
                     /translation="MLGKGLESLIPPKAGNGQENNGNAVPPIFPGNQPPAPAFMNPNS
                     HVDPFASSADANPMTNIVPETPAANPVPVPIENLEPMIPRPENNPEPVSSMSSQAMIP
                     EIKTSPILRETKTPVYDDDLADKKSEAVFQIEVEKIVPNPEQPRRHFDEESLRELASS
                     IREFGIIQPLIVSKVSNETESGTEVYYELIAGERRLMAAKMVGLRTVPAIVKHVSVGR
                     EKLEMAVIENIQRSDLNPIEEARAYARLQDEFRMTQREIASRLGKSRETVANAMRLLN
                     LPSEIQAALETGQLNESQGRLLLQVGDIKRQMEIFYSILTDKPSVRALKQRIRGETVP
                     RTEIRSIDPTLEAIKSRLEEILGTKVDIRAEGDKGKITINFYSSEELSSLIDKLVRQN
                     DNRSL"
     gene            complement(4270..>5907)
                     /locus_tag="PKG74_00680"
     CDS             complement(4270..>5907)
                     /locus_tag="PKG74_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOB89833.1"
                     /translation="SLISRAYFFAESCHKNQKRASGELYVNHCLKTAQNLADWNLDET
                     TVSAGLLHDVVEDTGCSLEEIDKNFGPQIANLVDGTTKLGRIKYRGDEKQKDNIKKLI
                     LATSKDLRVIFVKLADRLHNMQTLKSLPPDKQKRISLETYEIYSSLAYRLGMQRLAGE
                     LEDLAFPYLYPEEYKWLISNMKTRYAEREKYLKRIKPVVIKELTLANIKPLKVDFRAK
                     RYSSLYKKLLRYDMDIDKIYDLVAFRIIVENIQDCYAALGVIHKLWPPLPGRIKDYIA
                     MPKPNGYRSLHTVVFCVGQKIVEFQIRTMEMHEEAENGVAAHWAYEESKSSGKKRVSF
                     AKNQDISWVERLRAWQEEEADSPDFIDALKMDFFKDRIFAITPKGQVVDLPAGATPID
                     FAYQIHTDLGNQCVGAKVNGKIVPLNHELRSSDIVEIVVHKGKKPSISWLEFVKTSSA
                     ISRIKKALKDKGPLFEPKQTELHIVAQARPDLIKDLSNVVSRNHLSLVNLSANPTGSG
                     FQTIKIRCDSSDRRKISQLIVKIKSLKGIKEIDYRFV"
BASE COUNT         1435 a         1425 c         1612 g         1435 t
ORIGIN      
        1 acgggctcgc cggacgtttt tggcttctcg actatgtcgt ggttttttcg gccaagtggc
       61 ttatttggct cgcgctcgcc gctttcgcga tcgcggtttg gcgctcgaaa agtttgagga
      121 cccgggccta tttcttttct ttggccgctt tgggagcgat tctttcgaga ggaatcttcg
      181 ccgaaatcgt ccgctacgcg agatacaatc cacggccttt cgaggaattg gggatcgatc
      241 cgcttttcgg acaatacgcc acggccagct ttccttcggg acacgtcgct tttcttttcc
      301 cgatagcact cgcggcttgg aagtacaata agaagctggg gatttggctt attgcggcca
      361 cgatcttgat gggtatctca agggtaatcg ccggagtcca ttggatgacc gatatcgccg
      421 ccgggttctt ggtcggcgcc atcggatttc ttgtggccca ggcgcttttg ccccgtcttg
      481 aaaagaaagg ggaagaaaag ctatcataat aaggccgaag aaaaacacaa aataaaaaaa
      541 tgaacgagaa aacaaagata ttcttggttc cgggagcgat tgttgtcgcg ggaatcatca
      601 ttgccatggc tgttctttat tccggaggag tgatcgacac gacaagccaa acggcccagg
      661 tgggagaggg cttcgccgcc ttaaaaagcc tgaaagccgt taacagcgat gaccatattc
      721 tcgggaacgc ctcagccaag gtgaagatcg tcactttttc cgacatggat tgcccgtatt
      781 gccagagatt caattcgacc atcaaagaaa tactcgatat ttacggagac aagatcgccg
      841 tggtctaccg gcattttcct ttgaccatgc ttcatccgga cgcggagaaa aaatccgaag
      901 cgaccgaatg cgcggctaag cttggaggca acggaggatt ttggagctac ttcgacaaat
      961 acgaggaaga agccgccgcc ggaaaaacat tcagcgagga acttcttctt gacgtggccg
     1021 cgtcaatcgg actcgacagg gaagatttct cgacttgcct tcgaagcgac gaaccgggga
     1081 aactcgtgaa agaggacgcg gataacgccg cggaaatcgg catcaccgga acgcctttca
     1141 gcatcattct ctcgaacaac aaaccggtgg atacgatcga aggcgcttat ccgatcgaag
     1201 ctgtcagaga gaaattggat caatacataa aataaaggaa caagcagatg gaaaaatccc
     1261 tcgccatggc gggggatttt tgacttgatg cgcgaatctt gttattttta ttgaagtttt
     1321 tgcccaggtg gcggaatggt atacgcgtac gactcaaaat cgtatggggt aactcgtgtg
     1381 ggttcaagtc ccaccctggg caccgatatt tatcgaaaat atttcggtta cggctgaccc
     1441 gatcggttat tgcgcctttc tttgtcttaa ggtagtcttc gtgaagaagc cacatcggct
     1501 tatgtaccct gaaaaccgga aaggaagtgt ggaagtgacg aaagagaagg aaaagaaagc
     1561 gggagggctg tccgcgcgag agcttttgga ggctgtctac aaccttttga agaaagacgg
     1621 tagagaaatc aaactgcctt cgaaggctat ggccgagatt gccaatgact ccgatgggca
     1681 ccggacgcgt atcggctaca ccggatacga gtcggccgcc attctgaaag tggccgaccg
     1741 agaatggatc ttagccttcg ggacaaagtg cggacagtat ccagctgatc cttacaattg
     1801 cgacattgtc gccatgcggg ccaacctcga cggaaagacc gacaaagaag cggaacccga
     1861 aattcaccaa gctctcgaga gaaacacttg tttccaccat tcgctcgctt atgcgatggc
     1921 ggacgggacc cttgccttga accaaaacgg atgtttttgc gacaaggtga tcgaactcct
     1981 gcgaccgaag ataggggagt tcatcgctcg ggatctggag gtagatccga actgtttcac
     2041 catggacttg cggccggtgg tgaagtccgc cgtccaatac aagccggagt tcatagattt
     2101 tcttcgcgac acgttccttg cggttctcgc ctccgcctaa tggcgatcct ttttgcccct
     2161 gcggaattca tcggcaatga tgaatcccaa aggggctttc tttttatgtt caggaaattc
     2221 gaaatttttc ggaaaaatgc tttttttaca agaagtacca aggctcgctt ttggacatcg
     2281 agaaaggtaa aatacattta tggcgcgcgt gatcgcgatt tgcaaccaaa aaggaggtgt
     2341 cggcaagaca acgaccgccg tcaacctcgg ttcgtatttg gccgccttcg gccgcaaggt
     2401 tttgcttatc gatttcgatc cgcaggcgaa cgcgagttcg gctttggggt attcttcgaa
     2461 tgacggcgga accatttacc agggccttat cggagaagcg gaaccgcaaa aactcatccg
     2521 gccgacgatg attctgaatt gccactttat cccttcggcc agtcatttgt ccggcgctct
     2581 ggtggaattc gtcaatttgc cggagcggga atattttttg aaaaaattca tcgataaggt
     2641 aaggaacgct tacgattaca tcttgatcga tcttccgcca accttgagtc tcttaatgct
     2701 gaacggattg ctcgcttcgg gcgaggtgat cattccggtc caggcggaat attacggact
     2761 cgaaggattg agccagcttc tttcgacgct cgaactcgta cgatcgaatt tgaatcacga
     2821 tctcaaagtg gcaggagccc tgattaccat gtatgataag tgggaacatc tttcgcagga
     2881 agtggctaaa aacatcaggc ggaatttccc ttattatgtt ttcggggtgg aaataccgcg
     2941 gtcggtaagc ctggccgagg cgccgagctt cggtaagccg atactgcttt acgcgccgta
     3001 ttcccaggga gccttggctt atcgcaggct cgcttcggaa gtgatgcagc aggaaaatcc
     3061 gattacggcc gaaacgaaag aacaaaccgg aaacaatatg tgaaatgttg ggtaaaggac
     3121 tcgaatcttt gattccgccg aaagcgggaa acggacaaga aaacaacgga aacgcggttc
     3181 cgccgatttt tccggggaat cagcctccgg cgccggcttt tatgaatccg aacagccacg
     3241 tcgatccgtt cgcttcgagc gccgacgcga atcccatgac gaatatagtt ccggaaacgc
     3301 ctgcggccaa tccggttccc gttccgatcg agaatttgga gccaatgatt ccgcggccgg
     3361 agaataatcc cgaacccgtc tcgtcgatga gtagccaggc gatgattccg gaaatcaaaa
     3421 cctcgccgat tttacgagaa acaaaaactc cggtttacga cgatgatttg gccgacaaaa
     3481 aatccgaagc ggttttccaa atcgaagtcg agaagatcgt tccgaatccc gaacagccga
     3541 gaaggcattt cgacgaagaa tctttaagag agctcgcgag ctccatcagg gaattcggaa
     3601 tcattcaacc actgatcgtt tccaaggttt ccaacgaaac ggaaagcggc accgaagttt
     3661 attacgagct gatcgccgga gaaagacggt tgatggccgc caaaatggtg gggcttcgga
     3721 ccgtgccggc gatcgtcaaa cacgtctccg tcggccgcga aaaactggaa atggccgtta
     3781 tcgagaacat ccagcgttca gatttgaatc cgatcgaaga agcgcgggct tacgccaggc
     3841 tccaggacga attccggatg acccagcggg aaatcgccag ccgtttgggc aagagccgcg
     3901 agaccgtggc gaacgccatg aggcttttga acttgccttc ggaaatccaa gcggctttgg
     3961 aaaccggaca gttgaacgaa agccagggga gactgcttct tcaggtgggc gacatcaagc
     4021 gccagatgga gattttctat tccattttaa cggacaagcc gagcgttcgc gcactgaagc
     4081 agcgcattcg gggagaaacg gttccaagga ccgaaatccg ctcgatcgat ccgacgctcg
     4141 aagccatcaa aagcaggctc gaagagatcc tgggaaccaa ggtggatatc agagccgaag
     4201 gcgacaaagg aaaaatcacc atcaattttt attcgtctga agagttgtcg tctttgatcg
     4261 acaaactcgt cagacaaaac gataatcgat ctctttaatc cctttgaggg atttgatttt
     4321 gacgatgagc tgagagattt tccggcggtc ggacgaatcg catctgattt tgatcgtttg
     4381 gaatccgctg ccggtcgggt tggccgaaag attaaccagg ctcaaatggt tgcggcttac
     4441 gacgttcgac aagtctttga tcaagtccgg ccgagcctgg gcgacgatgt gtaattccgt
     4501 ctgcttcggc tcgaaaagcg gccctttgtc tttgagggct tttttgatgc gacttatggc
     4561 cgacgaggtt ttgacgaatt cgagccagga aatcgagggc tttttccctt tgtggacgac
     4621 aatctccacg atgtcgctcg atcggagttc gtggttcagg ggcactatct tgccgttcac
     4681 cttggcgccg acgcattggt ttccgagatc ggtgtgtatc tggtaggcga aatcgatcgg
     4741 agtggctccg gccggcaaat cgaccacctg gcctttgggg gtgatggcga aaatgcggtc
     4801 tttgaaaaaa tccattttca gagcgtcgat gaaatccggc gaatcggctt cttcctcttg
     4861 ccaggcgcga agccgctcca cccaggaaat atcctgattc ttggcgaagg agactctctt
     4921 tttccccgag gatttcgatt cttcgtaagc ccagtgggcg gccacgccgt tttccgcctc
     4981 ttcgtgcatt tccatggttc tgatttggaa ctcgactatt ttctgcccga cgcagaagac
     5041 aacggtgtgc aggctacggt agccgttcgg tttgggcatg gcgatgtagt ctttgatccg
     5101 tccgggaaga ggcggccaaa gcttgtggat gacgccgaga gcggcgtagc aatcctggat
     5161 gttttcgaca atgatgcgga aagcgaccaa gtcgtagatt ttatcgatgt ccatgtcgta
     5221 ccgcaggagt tttttgtata ggctcgaata ccgcttggcc cggaaatcga ctttcagagg
     5281 tttgatattc gcgagcgtca gttctttgat aacaaccggt tttattcttt tcagatattt
     5341 ttcccgttcg gcgtagcggg ttttcatatt ggagataagc catttgtatt cctccggata
     5401 aagatagggg aaagcgagat cctcgagttc gccggcgagt ctttgcatgc cgagcctata
     5461 ggcgagcgaa gagtatattt cgtaggtttc gagactgatc cgtttttgtt tatccggagg
     5521 cagggatttt agggtttgca tgttgtgcag cctgtcggcg agtttcacga aaatcacccg
     5581 aaggtctttc gaggtggcca aaatcagctt tttgatgttg tctttttgct tctcgtctcc
     5641 gcgatattta atgcgtccga gcttggtcgt gccgtcgacc aggttcgcga tttgcggtcc
     5701 gaagttcttg tcgatctctt cgagcgagca accggtgtct tcgacaacgt cgtgcaaaag
     5761 cccggcgcta acggttgtct cgtcgagatt ccagtccgcg aggttttggg cggtcttcaa
     5821 gcagtggttg acatagagct ctccggaggc tcttttctga ttcttatggc aagattccgc
     5881 gaaaaaataa gcccgggaaa taaggga
//
LOCUS       DAOPQT010000016         5903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_14765_length_5903_cov_3.186047, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000016 DAOPQT010000000
VERSION     DAOPQT010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14765_length_5903_cov_3.186047"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKG74_00685"
     CDS             complement(<1..325)
                     /locus_tag="PKG74_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89834.1"
                     /translation="MTVKRQYRETPEERAERKSHSSGIMVIEPAEKCSLVEFLGELEA
                     AGYEMVDALYKERIDGKDLCGKRTYHMVRFLFARREFVELSDEFKKVRDVIRAAEGEV
                     VRDVGF"
     gene            complement(661..735)
                     /locus_tag="PKG74_00690"
     tRNA            complement(661..735)
                     /locus_tag="PKG74_00690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(700..702),aa:Arg,seq:gcg)
     gene            825..1538
                     /locus_tag="PKG74_00695"
     CDS             825..1538
                     /locus_tag="PKG74_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOB89835.1"
                     /translation="MAKNYKKDFSYSYAIGVFPVIELLRNKPDKATRVYIHPKGERSA
                     GIKEILDICGKENIPVEENGRFIEKVSSSENVYAAAVFNKYESRLNLEANHVVLVNPS
                     DPGNLGTICRTMLGFGFSDLGIIKPGTDIFNPKTIRASMGAIFQLNFQYFSSFEDYRK
                     NFSQNVYVFMTDGKETLGRFSFKKPYVFVFGNEGEGLDQKFKKIGESIRIPQSGKVDS
                     LNLSAAVAVSLYEAMKTNK"
     gene            complement(1554..2495)
                     /locus_tag="PKG74_00700"
     CDS             complement(1554..2495)
                     /locus_tag="PKG74_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89836.1"
                     /translation="MTDRDRVLESLKAAPDIPVSELITFILDLRSVIYTSYKEDELKA
                     FQSLFPDTYIEKGSKEMCGEAGEVALISKSHDLAIKASRSLRDSRCLGALLGYPDCCI
                     EKHLTNIIADPPVRGAQTIFDSFVSRSTALFLTNNLLNFATRLDSKEDLEQNSFYHQK
                     NNFYLNQFFYLDSDVNPCLIWFAAISFISHLPCRYDCEKSVKIGRKVAKWMKENIPEV
                     FVFFKNTLQRPFLVFDTFDWIAFEGNVENGFLRYSKITPPFGVIDSQIKQNLNLGNRL
                     KADKDKIVIFKDDRPVFEYIKKDEKDGYLMNFVDCEI"
     gene            complement(2506..2994)
                     /locus_tag="PKG74_00705"
     CDS             complement(2506..2994)
                     /locus_tag="PKG74_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89837.1"
                     /translation="MKNKNIIFILVLIAAIGLISYYALKTKPASLEYKNSQYGFSFDL
                     PLSWQGYTIVESVWQGHIPGETGDIFSEEGPLISVRHPLWTEEKPRQDIPIMVFTIDQ
                     WDKVIADDLFVSAAPIPPMELGRNQNYVFALPARYNFAFPEGFEEVEQIIAAHPLRAF
                     DK"
     gene            complement(3019..3966)
                     /locus_tag="PKG74_00710"
     CDS             complement(3019..3966)
                     /locus_tag="PKG74_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89838.1"
                     /translation="MSDRDRVISCYSAEYTPDIYISELISFIIGIKPVIYADYREVEI
                     ENFRSIFPEAHIVKASKNILGEIRQIALISKDKYLAEQAAENILNSSELGKLLGYPEC
                     CVKRHIERTTRAPYIRGRKLIFDIFSPNRSAPFVINNLLNFPTRLDTPEDFKNADQYW
                     RGNSDNESLKYSINAKGENKEIFLLNISFIPHLPCRSDCAKSVEIGRLVAAWMKNNIP
                     ELFDFFKNILQRSFLVFDTFDWIAFDGKIKDNRLEYAGIAAPFTFMENPVKQNLPLGN
                     SLSAEKDRIVVFKDDRPVFEYEKKSEEDGYLVDFVDFEI"
     gene            complement(4104..4772)
                     /locus_tag="PKG74_00715"
     CDS             complement(4104..4772)
                     /locus_tag="PKG74_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89839.1"
                     /translation="MSKKCLAVPQRAVEALDFWKRLERDDNVIRSAITLSSDEVDDLL
                     ATARREGRFLERSGKDGIEQNPEWQQIIFYALVVQKGRFFVYKRSGAVDESRLALKLS
                     AGIGGHIEPYDTDLVDSLPRELDEKLLFTRRIQELPTPDKINIVGLIKDERDQVGQVH
                     LGLVCVVYLKADVSVEIKGDESVEGWMITLDEYERLIASGDYTPEGWTELLIEKLAAE
                     ILAQ"
     gene            4886..5308
                     /locus_tag="PKG74_00720"
     CDS             4886..5308
                     /locus_tag="PKG74_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009591741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOB89840.1"
                     /translation="MQPWIIYSLLSAIFAALVAILGKIGIQNIDSTLATTVRAFIMAG
                     FLFLTVLFSGKLPLLGTIQSGPLLFIVLSGIAGAISWLFYFLALKQGTATGVAALDRL
                     SIVFVLIFSLLFLGQKLTWKAGLGATLMAIGAILMTLQ"
     gene            5354..>5903
                     /locus_tag="PKG74_00725"
     CDS             5354..>5903
                     /locus_tag="PKG74_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOB89841.1"
                     /translation="MIVQIYEINSPESAKEVSEIGVDHIGVLVGNGKYPREIPIEKAK
                     EVFSAISAGSKKVALTLSDNLEEIFEIVDELKPDILHLGADEDALPLSRARQIRERYP
                     TLKIMRTVAVSGKESIAIAKSYEEIADYLLLDTYKSRLGATGQVHDWNISREIVRTVK
                     IPVILAGGLGSDNVVEAIQKVNP"
BASE COUNT         1565 a         1336 c         1361 g         1641 t
ORIGIN      
        1 cgaagccaac gtctcgcacc acttcgcctt ccgcggcgcg gatgacgtca cgtaccttct
       61 tgaactcgtc ggagagctca acgaactcgc ggcgagcgaa caagaagcgt accatgtgat
      121 acgtcctttt gccgcacagg tctttgccgt cgatacgttc cttatagagc gcatcgacca
      181 tctcgtatcc cgccgcttcg agttctccga gaaactccac gagggagcac ttctcggctg
      241 gctcgatgac cataatgccg ctcgaatgac tctttctttc cgcccgctcc tcgggagttt
      301 ctcgatactg gcgtttcacc gtcatcggta ttacgtcagg gttcgagaaa ctgaactgta
      361 gtgtaaccag acggaccccg ttcagtgcga ccacgacgga cactctctcc tttcgtaggt
      421 gtttttagat taattcgtgc agttttgcca ataaaaactt aggaattaca agcaaaactg
      481 cacgaagtat cttattaaaa agagcgatag tcttatatta aaataacagc atattatgtt
      541 atatttgtca aaccttttga gatgataaag gaatttcagc ttaaaagatt ttttaataaa
      601 taaatttctt cttttaaagt ttctctaaaa agacataaaa acgccttacg gcggaatata
      661 tggtgccccc gggaggaatc gaacccccat tacgagatcc gcaatctcgc gtcctatccg
      721 ttgaacgaca agggcaacga ttaaagatta ggcgctttag agccaaaaat caacgagtcc
      781 gcattgcgac aactcttgga ttccgatatt cttgtttatg tatcatggcc aaaaactaca
      841 aaaaagactt ttcctattcg tacgccatcg gggtttttcc ggtgatcgaa cttttgagga
      901 acaagcccga taaagccacc agggtttata ttcatccgaa aggagaaagg agcgccggca
      961 taaaagagat attggatatt tgcggaaaag agaatattcc ggtcgaggaa aacggccgct
     1021 tcatcgagaa agtctcttcg agcgagaatg tttatgcggc cgctgttttc aacaaatacg
     1081 aaagccgctt aaacttggaa gccaaccacg tggttttggt gaatccgagc gatccgggaa
     1141 atctgggaac gatctgccgg accatgctcg ggttcggttt ctccgacctc gggatcatca
     1201 aaccgggaac ggatattttt aacccgaaaa cgatccgggc gtccatgggc gcgattttcc
     1261 aattgaattt ccaatatttt tcttcattcg aggattatcg aaagaatttc tcgcagaatg
     1321 tttacgtttt tatgaccgac ggcaaagaaa ctttggggag attctctttc aagaaaccct
     1381 atgtctttgt ttttgggaat gagggagaag gacttgatca gaagttcaaa aaaatcggag
     1441 aaagcataag aatcccgcaa agcggcaaag tggattcttt gaatctttcg gcggccgtgg
     1501 ccgtcagcct ctacgaggcg atgaaaacga ataaataagt cggccgacgc tttttatatt
     1561 tcacaatcta caaaattcat caggtagccg tctttttcgt cttttttgat atattcaaag
     1621 acgggccgat catccttaaa aataacgatt ttgtctttat ccgccttcaa tctgtttccg
     1681 agattcaggt tttgttttat ttgactgtcg ataacgccaa acggaggagt gattttagaa
     1741 tatcggagaa agccgttttc cacatttcct tcaaaggcga tccagtcaaa ggtgtcgaaa
     1801 accagaaaag gcctttgcaa agtatttttg aaaaaaacga aaacttcggg aatgttttct
     1861 ttcatccact tggccacttt gcggccgatc ttgacgcttt tttcgcaatc gtatcgacag
     1921 ggaagatgag aaatgaagga gatggcggcg aaccaaatta agcagggatt tacatccgag
     1981 tccagataaa aaaactggtt aagatagaaa ttattttttt gatgataaaa agagttttgc
     2041 tccagatctt ctttactatc caatctcgtg gcgaagttca ggagattatt cgtcaaaaaa
     2101 agggcggttg atcgggagac gaaagaatca aatatggtct gggctcctct gaccggggga
     2161 tcggcgataa tattcgttag atgtttttcg atacaacaat caggataacc cagtaaggcg
     2221 ccgaggcatc tcgaatctcg aagactccgg gatgctttga tcgccaagtc atggctttta
     2281 gagatcaaag cgacttcgcc ggcctcaccg cacatttctt tactcccttt ttctatatag
     2341 gtgtccggaa aaagcgattg gaaagctttt aactcgtctt ctttatacga ggtatagata
     2401 acggaacgga gatccaggat aaaggttatc aactcggaaa cgggaatgtc cggcgccgct
     2461 ttcaaggatt ccaaaacacg atcgcggtca gtcattcaag aagtgttatt tatcgaaagc
     2521 acgaagaggg tgggccgcta ttatttgctc aacttcttcg aagccctcgg gaaaggcgaa
     2581 attgtatctc gccggcaagg cgaagacata attttggttc cggccgagct ccatcggagg
     2641 gatcggcgcc gcggagacaa aaagatcgtc agctattact ttgtcccatt gatcgatagt
     2701 gaaaaccatg atcggaatgt cttgcctcgg tttttcttcg gtccatagag gatgtctgac
     2761 ggagataagc ggcccttctt cggaaaagat gtctccggtt tctccgggga tatgcccctg
     2821 ccaaacggat tcgacgatcg tgtatccttg ccagcttagc ggcaggtcga acgagaaccc
     2881 gtattggctg tttttatatt cgagagaagc gggttttgtt ttcaaagcgt aatagcttat
     2941 gagaccgatc gcggcgatca agacgaggat gaaaataata tttttgtttt tcatatatcg
     3001 aattttatct ccgaaaactc aaatctcaaa atccacgaaa tcgaccagat aaccatcttc
     3061 ctcgcttttc ttctcgtact cgaaaaccgg ccggtcgtct ttaaaaacga cgatcctgtc
     3121 tttttccgcc gaaagactat ttccgagagg tagattctgt ttcaccgggt tttccataaa
     3181 agtgaacggc gccgcgatcc cggcatattc caaacggttg tcttttattt tgccgtcgaa
     3241 ggcgatccag tcgaaggtgt cgaaaaccaa gaaagacctt tgtaagatat ttttgaagaa
     3301 atcgaaaagt tcgggaatgt tatttttcat ccaggcggcc accaagcgtc cgatctcgac
     3361 gctctttgcg caatcagatc ggcaaggcaa gtggggaatg aaggagatat tcagtaaaaa
     3421 gatttcttta ttttctcctt tggcattaat cgagtatttt aaactttcgt tatcgctgtt
     3481 tcctctccaa tattggtcgg catttttaaa atcttcgggc gtgtcgagcc tggtcggaaa
     3541 attaagaaga ttattgatga caaacggagc cgatcgattc ggagaaaaaa tatcgaatat
     3601 caatttgcgt ccgcggatat aaggagcccg ggtcgttctc tcgatatgcc ttttgacgca
     3661 gcactcggga tatccaagaa gctttccaag ctcggaacta ttcaaaatat tttctgccgc
     3721 ttgctccgct aaatatttat ccttggagat cagggcgatt tgtcttattt cccctaagat
     3781 gttcttgctt gctttgacga tatgcgcttc cggaaaaata gagcgaaaat tctcgatttc
     3841 aacttcgcga taatccgcgt aaattaccgg ttttataccg ataatgaaac taatcagttc
     3901 ggatatataa atgtccgggg tatattcggc gctgtaacag gagatgacac gatcccggtc
     3961 gctcatttgg ttttatgtat catggccgta tttgctttcc gggcgccggc attgttatga
     4021 cgcattttac atagataaaa ttacacgaaa aagccttttt taacaacaaa aaggccatac
     4081 aattgtatgg cctcggtccc gcttcattgg gccaagattt cggccgcgag cttctctatc
     4141 aaaagttctg tccaaccttc cggagtatag tctccggagg cgatcagccg ttcgtattcg
     4201 tcgagagtga tcatccatcc ttcgacgctt tcgtctcctt tgatctcgac ggaaacatcc
     4261 gctttaagat agacgacgca tacgaggccg aggtgtacct ggccaacctg atccctttcg
     4321 tctttgatca gtccgacaat attgatcttg tcgggtgtcg gcagctcttg gatccttctc
     4381 gtgaaaagca gcttctcatc caattctctc gggagagaat cgacgaggtc ggtgtcgtag
     4441 ggttcgatat gcccgccgat tccggcggaa agcttcaggg ccagccttga ttcgtctacg
     4501 gcgccgcttc tcttgtagac gaagaatcgt cctttttgga caacgagcgc gtagaaaatg
     4561 atttgctgcc attccggatt ttgctcgatt ccgtcttttc cgcttctctc caagaatcga
     4621 ccttctctgc gcgccgtcgc gaggagatcg tcaacttcgt cgctagacaa ggtaatggcc
     4681 gatctaatca cgttgtcgtc tctttccaat ctcttccaga aatccaacgc ctccacagct
     4741 ctttggggca ccgccagaca ctttttggac atcttaaaga ccctccttct tcacaatccg
     4801 aacgggtttt attgtagggc aaagcggatg ttgcggcaac gactccgttt ttctaaaaga
     4861 accgaaagga tgttatattg aaaccatgca gccctggatt atttattctc tactgtcggc
     4921 gattttcgcg gctttggtcg ccattctagg aaagatcgga attcagaata tcgacagcac
     4981 tttagccact accgttcgcg cctttatcat ggccggattt ttattcttga cggttttatt
     5041 ttccggaaaa ttgccgttat tgggcactat ccaaagcggc cctttgttat ttatcgtgct
     5101 ctcgggtatt gccggcgcca tatcctggct cttttatttt ctggctctca aacaaggaac
     5161 agccactggc gtggccgctt tggatcgttt gagcatcgtc tttgtactga ttttttcttt
     5221 gttgtttttg ggccaaaaac ttacctggaa ggcgggctta ggagcgactc ttatggctat
     5281 cggcgcgatt ctaatgactc ttcaataaag acatatttgt ccttcaaggc tcttggaatc
     5341 cgaccgtaaa aatatgattg tccaaatcta cgaaatcaac agtcccgaat cggccaaaga
     5401 agtctcggaa ataggagtgg atcatatcgg tgtattggtc gggaatggaa aatatccgag
     5461 agagataccg atagaaaaag cgaaagaagt tttttccgcc atttccgctg gatcgaaaaa
     5521 agtcgctctg actttatccg ataacctcga ggagatattc gaaatcgttg atgaattgaa
     5581 acccgatatt ctgcatttgg gcgcggacga ggacgcgctg ccattaagca gggcaaggca
     5641 aatccgcgag agatatccaa ccctgaaaat aatgagaacc gtcgccgtca gcgggaaaga
     5701 aagcattgcc atcgccaaga gttacgagga aattgctgat tatctgcttc tggacactta
     5761 taaatcccga ttgggagcga ccggacaagt ccacgattgg aatataagca gggagatcgt
     5821 tcggaccgtg aaaatacccg taattttggc cggaggattg gggtcggaca atgtcgtcga
     5881 agctattcag aaggttaatc cgg
//
LOCUS       DAOPQT010000017         5850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_14953_length_5850_cov_4.014495, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000017 DAOPQT010000000
VERSION     DAOPQT010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14953_length_5850_cov_4.014495"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /gene="metG"
                     /locus_tag="PKG74_00730"
     CDS             <1..397
                     /gene="metG"
                     /locus_tag="PKG74_00730"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOB89842.1"
                     /translation="WPEKNPKSESRSSWPWPRNIRSTMISIDDFKKIELKTAKIIQAE
                     HVEGSEKLLKLRVTLGDEERQIIAGIGKYYEPDDLVSKNIVVVANLEPKTLAGEESQG
                     MLLAATDLVGGEPVIISPIKETAAGSIIK"
     gene            403..930
                     /locus_tag="PKG74_00735"
     CDS             403..930
                     /locus_tag="PKG74_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89843.1"
                     /translation="MVLLPHAVVGAAIGVAVKNPLWSFLIGIVSHHLMDSFPHLDYGS
                     RLIKKSGPRFLGHKPKIKSDSPRKFDETFWKILFVDFSVAWTIFLWIFYRLPEDLWLS
                     VFFGALGALFPDVLSFYPPFVKAVTRKYKWAARIAEIHSVFHWALPVKPLFWGLFWQI
                     AFTASALVYLSRFLQ"
     gene            973..1806
                     /locus_tag="PKG74_00740"
     CDS             973..1806
                     /locus_tag="PKG74_00740"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOB89844.1"
                     /translation="MKKILPIVLKIIIAIAVFSLAVVYVPKLFGQGGLNIFAPRKSES
                     GNVIVREPVANGLKSLPFSVKGEARVFENMIAYELRDAADGFVLSEGNAYANAPDVGR
                     FGPFSVEIKSLRRLPMDGGVILEVFNYSAKDGSRENQVVVPLTLAKQDFQTLQVFFGK
                     QGISEADCMTMAAVPRIVPKTSAPGRESLELLLEGPTRTEAAEGYYTNLNPDVKIQRL
                     AIENGLAEVDFDETLQRSVGGSCRVTAIRSQIIKTLKQFSTVENVVISIDGRTEDILQ
                     P"
     gene            1866..2408
                     /locus_tag="PKG74_00745"
     CDS             1866..2408
                     /locus_tag="PKG74_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89845.1"
                     /translation="MAQKEKVVVLNKPETRRKILVGVIIALVVIIPFVYWKGWSRLQT
                     AFDQIMVERTVKNFYRDFAEQINTNEAWEDFIEKYLSKSAETLMTRRASRLVYDAEHG
                     SQHVFKFTSPVETKVYKLEDLMAYVLADAAYEESLIGGEPYDFVMEKFLTLEKINNEW
                     KITADADRTASFNEYIESLN"
     gene            2410..3444
                     /locus_tag="PKG74_00750"
     CDS             2410..3444
                     /locus_tag="PKG74_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89846.1"
                     /translation="MTKYKIYFAAGLLFALTFAGADLKTAKADYVTMINGEPAEVKYN
                     ESTGKLDVYRVGEDEPFAEGVSFKQDESTNNRGVLDMDDLADKASKNNVTIQDTEVKA
                     PTRQEQGGVMDSTEEADDYSCRQVSSCGNNVIGYGSENPKDPNYALYSSTDVQEIWTV
                     YLRNSGQYTGNDAWDQAGKDASKYLQEHPELNKATLTAEEANNLLTDLGANFQVDKNI
                     ENVSRLDLLKAIDQAGRAAEEQEPGEEEGEPWTPPETGPFVSVLDCSFTATPAEILYS
                     KSSRLRWNCNRADTCSISDETGVVIRSSIVTMSGEVQVTPLKTTRYFLNCSDNKSWET
                     LVRVFSSGIQ"
     gene            complement(3519..3749)
                     /locus_tag="PKG74_00755"
     CDS             complement(3519..3749)
                     /locus_tag="PKG74_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89847.1"
                     /translation="MRIIRRVLFVIGVWLTVELVAWAVFSALGQAMFQTRYVGIGQFM
                     PTPTMMGTGPVLLLVTWRKTWPEVQRFIDSTF"
     gene            3852..5156
                     /gene="murA"
                     /locus_tag="PKG74_00760"
     CDS             3852..5156
                     /gene="murA"
                     /locus_tag="PKG74_00760"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013944543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOB89848.1"
                     /translation="MPQKFIIKGGNPLKGEVRVRGAKNSISKLLIASLLTEDEVKLTN
                     VPLNQETEISLELCENIGSIVKREADTIVIRTPDIKNYRVKELSRKNRIPILALGPLL
                     VRVGEAEVPMVGGDKIGPRPIDFHIASLEKLGAKIELNESTIVAKAKTCLCGADIVLP
                     YPSVGATENSILAAVLAKGRTTIVNAATEPEIIDMIKMLQNMGAIIELGANRTVYIDG
                     VERLHGVEHRLIPDRLEAVSFAVMALATNGDILVKDAVQEHLIAFLNACRRMGAKYVV
                     EPEDGIRFYRNGELKAANIETDTYPGFATDWQQPTAVLLTQARGTSVIHETVYEDRFG
                     YTEDLNKMGAKINVETKCLGILPCRFQGKNYKHSAVIQGPTPLKAADISMRDIRAGMA
                     QVIAALIADGVSEVSGVEHIDRGYEKIDERIKELGGDIKRID"
     gene            5164..>5850
                     /locus_tag="PKG74_00765"
     CDS             5164..>5850
                     /locus_tag="PKG74_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOB89849.1"
                     /translation="MTKQEVKERIEKLKKEINRDRYAYHVLDKSLIPDEVLDSLKKEL
                     FDLELQYPEFVTPDSPTQRVAGEPLKEFKKVPHERPMISFNDAFSEKDMEDWIDRLEN
                     YLKKKIDKEFFCELKLDGLSVELVYDNGVFVRGSTRGDGLVGEDVTQNLRTIEDIPLK
                     LEGDYPKHLVVRGEVILSKKEFERINKEQKKKGEKLYANPRNIASGSVRQLDPKITAS
                     RNLNSFQYDVV"
BASE COUNT         1648 a         1362 c         1502 g         1338 t
ORIGIN      
        1 ctggccggag aaaaacccga aatcggaatc aaggagttct tggccgtggc ctcggaatat
       61 tcgatcgaca atgattagca tcgacgattt caaaaaaatc gaattgaaaa cggcgaaaat
      121 aatccaagcc gagcacgtgg aaggatcgga aaaattgttg aagctccggg taacgctcgg
      181 cgacgaagaa aggcaaataa tcgccggaat cgggaagtat tacgagcccg atgatctggt
      241 ttccaaaaat atcgtcgttg tcgcgaatct cgaaccgaaa accctggccg gagaagaaag
      301 ccaaggaatg cttttggcgg ccacggatct agttggcgga gaaccggtga tcatttcgcc
      361 gatcaaggaa acagcggccg ggtcgatcat caaataaacg ccatggttct tctgccccat
      421 gccgttgtcg gagccgccat cggcgtcgcc gtcaaaaatc ctctttggag ctttttgatc
      481 ggtatcgtga gtcaccatct gatggactct ttcccgcatc tcgattacgg cagtcggctg
      541 atcaaaaagt ccggtccgag attcctggga cacaaaccga aaatcaaatc cgattctccg
      601 cggaaattcg acgaaacctt ctggaagatt cttttcgtcg atttttccgt ggcctggaca
      661 attttcttgt ggatttttta ccgtttgccg gaagacttgt ggctgtcggt ttttttcgga
      721 gctttgggag cgttgttccc ggatgttctc tctttttatc cgccgttcgt caaggcggtg
      781 acgcggaaat acaaatgggc ggcccgcatc gcggaaatac attcggtttt ccattgggct
      841 ttgcccgtca aaccgctttt ctgggggctt ttctggcaga tcgctttcac ggcatcggcc
      901 ttagtgtacc tgtctcggtt tttacaatga tcgatcgccc ctcggggaaa taaatgttag
      961 aataaaagca tcatgaaaaa gatccttccc attgtcctta aaatcatcat cgctattgcc
     1021 gttttcagtt tggcggtcgt ttatgtcccc aaactcttcg gtcaaggcgg attgaacatc
     1081 ttcgctccga gaaaaagcga aagcggaaac gtcatcgtgc gcgagcccgt ggccaatggc
     1141 ctgaaatccc ttcctttttc cgtcaaagga gaggctcggg ttttcgagaa catgattgcc
     1201 tacgagctcc gggacgctgc cgacggcttt gttttgagcg aaggcaacgc ttatgccaat
     1261 gcgcccgatg tcggccgatt cggtccgttt tccgtggaaa tcaaaagctt gaggcgtttg
     1321 ccgatggacg gcggcgtcat tctcgaggtt ttcaattatt cggccaaaga cggttcgcgg
     1381 gaaaatcagg tggtcgtgcc tttaacattg gccaagcagg atttccagac gctccaggtg
     1441 tttttcggga aacaggggat ttccgaagcg gattgcatga ccatggcggc ggtcccgagg
     1501 atcgtcccga aaacttcggc tccgggacgg gaaagtctgg aactcttgtt ggaagggccg
     1561 acgagaaccg aagctgccga aggctattac accaatctta atccggacgt taaaatccag
     1621 cgcctcgcga tcgagaacgg cctggccgaa gtcgatttcg acgaaacgct tcaaagaagc
     1681 gtcggcggtt cttgccgggt gacggccatc agatcgcaga tcatcaaaac cttgaagcaa
     1741 ttttcgaccg tcgagaacgt agtcatttcc atcgacggta gaaccgaaga cattctccaa
     1801 ccgtaatcgg tcgaaaccgc ttggtcgaat gtcgatatga tataatagaa aaaaacaaaa
     1861 caaacatggc acaaaaagaa aaagtcgttg ttttaaacaa accggaaaca aggcggaaga
     1921 ttctcgtcgg agtcatcatc gcgcttgtgg tcattattcc gttcgtctat tggaagggtt
     1981 ggagccggct tcagacggct ttcgaccaaa taatggtgga gcggacggtc aagaatttct
     2041 accgggattt cgccgaacag ataaacacga acgaagcttg ggaagatttt atcgagaagt
     2101 atctttcgaa aagcgccgaa accctgatga ccagacgggc ttcgagattg gtttacgacg
     2161 ccgaacacgg cagccagcac gttttcaagt tcacttctcc ggtggaaacc aaagtctata
     2221 agctcgagga tttgatggct tacgtcttgg ccgacgccgc ttacgaagag agcctgatcg
     2281 gcggagagcc gtatgatttt gtcatggaga aattccttac cctcgaaaaa atcaacaacg
     2341 agtggaaaat aaccgccgat gcggatcgaa cggcgagttt caacgaatat atcgaaagct
     2401 taaattaaca tgaccaagta caaaatatat ttcgcggccg ggcttctatt cgctttgact
     2461 tttgccggcg cggacttgaa aaccgccaaa gccgattatg tcaccatgat aaatggtgaa
     2521 ccagcagaag ttaaatacaa tgagagtact ggaaaactgg atgtataccg tgtgggagaa
     2581 gatgaaccat tcgccgaggg cgtgtctttc aaacaagacg aaagtaccaa taatcgcggt
     2641 gtgctggata tggatgattt agcggataaa gcatccaaaa ataacgttac aattcaagac
     2701 accgaagtta aagctccgac aaggcaagaa cagggggggg tgatggattc tacggaagaa
     2761 gcagatgatt attcttgtag acaagttagt tcttgcggta ataacgtaat tggttacggt
     2821 tcggaaaacc caaaagatcc gaattacgct ttgtacagca gcacagatgt gcaggaaata
     2881 tggacggttt atctcagaaa ctccggacaa tatacaggta atgatgcttg ggaccaagca
     2941 ggaaaagacg cgtctaaata tctacaagag caccctgaac ttaataaagc tactttaaca
     3001 gctgaagaag caaacaatct tttaacggac cttggtgcta attttcaagt agataaaaat
     3061 attgagaatg tctctcgtct cgatttgcta aaggctattg atcaagccgg cagagcggcg
     3121 gaagagcagg agccaggaga agaggaagga gagccgtgga ctccaccgga aaccggacct
     3181 tttgtttcag ttttggattg ttcattcacg gccacaccag cggaaatcct ttacagcaaa
     3241 tcaagccggt tgagatggaa ttgtaatcga gccgacactt gctcgatcag cgatgaaacg
     3301 ggtgtggtta taagatcgtc gattgtgaca atgtccggag aagttcaggt tactccgctt
     3361 aaaaccactc gttatttcct gaattgttcc gataacaaat cttgggaaac tttggtaaga
     3421 gtcttcagta gcggaataca gtaagataaa tgtaaagcga aaaccccgtg ctcatagtac
     3481 ggggtttttg aataaaaata gaacgctcta agagcgttct aaaaagtgct gtcgataaat
     3541 ctctgtactt ctggccaggt tttcctccag gtaactagga gcaaaacagg cccggttccc
     3601 atcattgtcg gcgtcggcat aaactgaccg atgccgacat aacgtgtttg gaacatagcc
     3661 tgacctaggg cgctgaaaac cgcccaagcc accaattcga cagtcagcca gactcctatg
     3721 acgaataaga ctcttcgtat tattctcaaa gtacctcctc ctttctaatc ttatattata
     3781 attataacat attttggcaa tcttgtcaaa catcaaaaac ataggaatta tgctattatt
     3841 taacacaata catgcctcaa aaattcatta tcaaaggcgg aaatccgctt aaaggtgaag
     3901 ttagggtccg cggcgccaaa aactccatca gtaagctttt gatcgccagt cttttgaccg
     3961 aagacgaggt gaaattgacg aacgttccct tgaaccagga aacggaaatc tctttggagc
     4021 tttgcgaaaa catcggttcg atcgtgaagc gcgaagcgga cacgatcgtc atcagaactc
     4081 cggatatcaa gaattatcgc gtcaaagaat tgtcgagaaa aaaccgcatt cctattttgg
     4141 ctttgggacc gcttctcgtc agagtcggag aagccgaagt gccgatggtc ggcggagata
     4201 agatcggccc ccggcccata gatttccata tcgcctcttt ggaaaaactc ggagcgaaaa
     4261 tcgaattgaa cgaatcgacc atcgtcgcca aggccaaaac ctgtctctgc ggagcggaca
     4321 tcgttttgcc gtatccgagc gtgggtgcca cggaaaactc tattttggcc gcggttcttg
     4381 ccaaaggcag gacgacgatc gtgaacgccg ccaccgagcc ggaaatcatc gatatgataa
     4441 aaatgctcca gaacatggga gcgatcatcg aacttggagc gaaccggacg gtctatatcg
     4501 acggcgtcga gcgcctccac ggagtggagc atcggctcat acccgacagg ttggaagcgg
     4561 tttctttcgc ggtcatggct ttggcgacaa acggcgacat cctggtcaag gatgcggtcc
     4621 aggagcattt gatcgctttt ttgaacgcct gccggagaat gggagcgaaa tacgttgtcg
     4681 aacccgaaga cggcatcaga ttttaccgga acggagaatt gaaagcggcg aatatcgaaa
     4741 ccgacactta tcccggattc gccaccgatt ggcagcagcc gacggccgtg cttctgacgc
     4801 aggcaagagg aacgtccgtc atccacgaaa cggtttacga agaccggttc ggctacaccg
     4861 aagatttgaa caaaatgggc gcgaaaatca acgtggagac aaagtgcctc ggaatcttgc
     4921 cttgccgttt ccagggcaag aattacaagc acagcgccgt catccaaggg ccgacgccac
     4981 tcaaagcagc cgacatttcc atgcgggaca tcagggccgg gatggcccag gtgatcgccg
     5041 ctctgatcgc cgacggcgtg tccgaagttt ccggagtgga gcacatcgac cggggttacg
     5101 aaaaaataga cgaaaggata aaggagctcg gaggcgatat taaaagaatc gattagactc
     5161 aagatgacga agcaagaagt caaagagcgc atcgaaaagc ttaaaaaaga gatcaatcgg
     5221 gatcgctacg cctatcatgt cctcgacaaa tctttgattc cggacgaggt tttggactcc
     5281 ctgaaaaaag aactttttga tctggagctc caatatccgg aattcgtcac gccggattcg
     5341 ccgacgcaga gagtggccgg agaacctctt aaggaattca agaaagttcc gcacgagcgg
     5401 ccgatgattt ctttcaacga cgctttttcc gagaaagaca tggaagattg gatcgacaga
     5461 ctcgagaatt acctgaaaaa gaaaatcgac aaagaatttt tctgcgaact gaagctcgac
     5521 gggttatcgg tggagcttgt ctacgacaac ggggtttttg tccggggatc gacaagaggc
     5581 gacggcctgg tcggcgaaga cgtcacccag aacttgagaa ccatcgaaga cattcctttg
     5641 aagctcgaag gtgattaccc gaaacatttg gttgtccgcg gggaagtgat tttgagcaaa
     5701 aaagaattcg aacggatcaa caaggagcag aaaaagaaag gcgagaagct ttacgccaat
     5761 ccgcggaaca tcgcctcggg ttcggtccgc cagctcgatc cgaagatcac cgcctcaaga
     5821 aacctgaatt ctttccagta cgacgtcgtg
//
LOCUS       DAOPQT010000018         5810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_15075_length_5810_cov_3.520938, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000018 DAOPQT010000000
VERSION     DAOPQT010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15075_length_5810_cov_3.520938"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /gene="cyaB"
                     /locus_tag="PKG74_00770"
     CDS             <1..522
                     /gene="cyaB"
                     /locus_tag="PKG74_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOB89850.1"
                     /translation="IGDLEQVKNELQKIGAEFVKKERQSDHIFGREKDLDGEHKIIEG
                     HFSARIREKGEKKLIEFKEINRNGIGMEFSSPLSSMGSGFNFLEKLDFTKAFSIVKNR
                     EVYKYQNFEICLDDVEQLGCFIEIESSSHEDGDRSEEIKKCRDLMGLIAPGAISESKK
                     YGDLMQELINKNQ"
     gene            581..1135
                     /locus_tag="PKG74_00775"
     CDS             581..1135
                     /locus_tag="PKG74_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOB89851.1"
                     /translation="MKDAVRFKDSKKMIGDSAAEHSWRTALMVFVLADELKIKINLTK
                     ALEMALVHDIVESVAGNIDHVSIVKGKITEKQKANLENKAINQLKKTMPRFSGNKIPA
                     LWQEYEKGTTREAKFVKAANKLETLMYLLESGSKSYDEPEIIFHYADHAVNNFPELKP
                     VLEELKKELKKEFKKGRISLERSR"
     gene            1294..1917
                     /locus_tag="PKG74_00780"
     CDS             1294..1917
                     /locus_tag="PKG74_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89852.1"
                     /translation="MALVVFASWIKLVSFGGPSVILNGVLLIPLTKSPHRRGRLGSRE
                     SQDWYASVRQISRVFDRLTCKRHRIPEKDIVLICDFQEASDQAEADLYAVAMEEFGIY
                     NPRIIRDGLETIRQVDVAFDLAAFEGKRIIFFGTWFHYLRILWIIRRHPKSKEVPWEL
                     RGAFGIPRPKEAVTDIILTLVFPILDLFGLRSWFVELTNRRRAEGRH"
     gene            1999..3024
                     /locus_tag="PKG74_00785"
     CDS             1999..3024
                     /locus_tag="PKG74_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOB89853.1"
                     /translation="MNLVEITSEKEFDPLAVNQSVPFTQSWFYGDWQAEEKREVRRFS
                     IEDGDEIIGSLQAIRYPLTFDKSYIYIPYGPVLKNNLTEKTAAFLKKSLKEVFNDGKT
                     VFIRLDFWPALKDRLEKTAGKFFVKSPRSSYRGSNFQPRAERVIDLKQPENEIFFAME
                     PKARYNVRLALKKGVVVKTITKNFLDYFEDFYSLMTETANRDGFSLHPRSYYEEIFNS
                     AEKRKNAFLALAFYGDTLLVANLVVLYGETAFFLFGSSSSEYRELMPSYLCQWQSLRK
                     AKELGFSYYSFGGISHKGLNRSWEGISVYKKKFGGFVREYSPFYDLAIQPFWYYLYIL
                     RKAFFGR"
     gene            3035..4345
                     /locus_tag="PKG74_00790"
     CDS             3035..4345
                     /locus_tag="PKG74_00790"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOB89854.1"
                     /translation="MKSFLRKLVPQPILGAYHYSMAKLGALVYGFPSRKMLIIGVTGT
                     KGKTSTANFIWGVLNSSGWKTGIVSTANMRIGDEEYPNVYHMTMPGRFEIQKWLKKML
                     EADCRAAVVETTSEGINQYRHKGIEYDFLVFTNLTPEHLPSHGGSFEKYKEAKGKIFV
                     DLAKTKRKVLQDLVLKKIFIVNNDSEYKDYFLNFEADKKITFGIRGQADYLAENIKTL
                     NEETSFDIGPGHYVISIPGNFNVYNALPGIVIGKELGFSSANIAVGLKQLKNIPGRME
                     KIDEGQDFAVFVDYAHEKESLTAVLETAKSMSPNRIVILLGAEGGGRDKSKRPVMGKL
                     AGEKADYVVVSNVDPYEDEPGPIIEDIAKAAEETGKVRDQNLFSIEDRRTGIRKALSL
                     ARKGDVVLITGKGAEQSIIIGGKKYSWDDRKVVREELEKILNKN"
     gene            4348..5460
                     /locus_tag="PKG74_00795"
     CDS             4348..5460
                     /locus_tag="PKG74_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89855.1"
                     /translation="MNKIKKILFVVLLIGIFCWVLYDWSDWKIEMNQEKVLSDEKNYT
                     EPAGESEKPGAAIDPQTLVWEKALSNAPWGPRDSQGGIVFDGKMWIFGGLRGFPEAEN
                     NYGVAPHFNDIWSSEDGANWILEKEHAAWSPRRSMSVEYFKGKLWMFGGWSTTTGYRT
                     GIWVSDDAVNWKQVLKDAPWGGREGQTSAVFQGKLWMFGGLSFGARLGDQNLKNDVWN
                     SEDGIHWNLVTENAGWAPRYDHGTAVFQDKLWVAGGVGHGKAMNDVWSSSDGVNWVLE
                     NGNAPWEERHGMPLVNYKDRLWLVSGWGTVNEAGTQDVWYSANGKDWQKAGIDFSWPG
                     REDHEVMVFQDKLWVMAGMGPFWVWNSDVWYSDFGR"
     gene            5476..>5810
                     /locus_tag="PKG74_00800"
     CDS             5476..>5810
                     /locus_tag="PKG74_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89856.1"
                     /translation="MPQIECEIRSFIDKQKYEELIEKFKKEAEFLGERDETTYYLDVP
                     QDLRIQRNDAYAKIWLKKGKLHDDAREEIEVRFSKEDFGKMEEMLSEIGIDPEIKWFR
                     HRNEFLWNK"
BASE COUNT         1691 a         1222 c         1523 g         1374 t
ORIGIN      
        1 atcggggatt tggaacaagt taaaaacgag cttcaaaaaa taggagccga attcgtaaag
       61 aaggaaagac agtcggacca tattttcggg cgcgaaaaag atctcgacgg cgaacataaa
      121 ataatcgaag gacatttttc ggcgcgaatc agggaaaaag gagaaaagaa gctgatcgaa
      181 ttcaaggaaa taaacagaaa cggaatcggg atggaattca gctctcctct ttccagcatg
      241 ggatccggct tcaatttcct ggaaaagctc gatttcacga aagctttttc tatcgtaaaa
      301 aacagagaag tttataaata tcagaatttc gaaatctgtc tggacgatgt cgaacaattg
      361 ggatgtttta tcgaaataga gtcttcttcc cacgaggacg gggatagatc tgaagaaata
      421 aaaaaatgcc gggatctgat gggtttgatc gccccgggcg cgatatctga gtccaaaaaa
      481 tacggcgatc tgatgcagga attgatcaat aaaaatcaat aaacgattca tggcagaaga
      541 tccatcaaaa ttaatcgggt ttttacacaa agcgggcgga atgaaagacg cggtaagatt
      601 caaggattct aaaaaaatga tcggcgattc cgcggccgaa cactcttgga gaacggcttt
      661 gatggtattc gttctcgccg acgagttaaa gatcaaaata aacctgacaa aggcgctgga
      721 aatggccttg gttcacgata ttgtcgaatc ggtggcggga aatatcgatc atgtttccat
      781 cgtcaagggg aaaatcaccg aaaaacaaaa agcgaatctg gagaataagg cgatcaatca
      841 gttgaaaaaa acaatgcctc gattctccgg caataaaatt cccgctttgt ggcaagaata
      901 tgaaaaaggg acgactagag aggcgaagtt cgtcaaagcc gcaaacaagc tggaaacttt
      961 gatgtatctt ttggagagcg gttccaaaag ctacgatgaa ccggaaatca tattccatta
     1021 tgccgatcac gcggtgaaca atttcccgga gctgaaacct gttttggaag aactgaaaaa
     1081 agagttgaaa aaggaattca agaaaggaag gatttctctt gaaagaagcc gctgaaagac
     1141 gaaatcaaaa taagaatcaa tgcccggttc ggccgggctt ttaacttgct cggaactcat
     1201 cggtgcttga caaatcatat attttatgct gtaaaaacag tattagttct ttgaaaggaa
     1261 tgatgagctt tggagataat ccttttggtt atcgtggcgt tggttgtctt cgcgtcctgg
     1321 atcaagcttg tctcgttcgg cggcccgtcg gtgattctca acggggtttt acttataccc
     1381 ctgaccaagt ctccgcatcg tcgcggcagg cttgggtcta gggaaagcca ggattggtat
     1441 gcgagcgtgc gtcagatcag ccgcgtcttc gacagattga cgtgcaaacg tcataggatt
     1501 cccgagaaag atatcgtcct aatctgcgat ttccaagaag catcggacca agccgaagct
     1561 gatctctatg ccgtggctat ggaagagttc gggatatata acccgaggat catccgggac
     1621 ggcttggaaa ccataagaca ggtcgacgtc gcctttgatc tggcggcgtt cgaggggaaa
     1681 aggataatct tctttgggac gtggttccat tatctgcgga ttctctggat catccggaga
     1741 catccgaaat ccaaggaagt cccttgggag cttcgaggcg ccttcgggat tcctcggccg
     1801 aaagaagcgg tcacggatat tattctgacc ctcgtcttcc cgatattgga ccttttcggc
     1861 ttgcggtcgt ggttcgtgga attgaccaac cgccgccgag ccgaaggaag gcattgaagt
     1921 tctccacacg gtccctgatg ggcatatgag tatatgctta tcaggggctt ttttgttttt
     1981 gtatgctaaa gtattcttat gaatttggtc gaaatcacct cggaaaaaga attcgatccg
     2041 ctggccgtaa atcaaagcgt gccttttacc caaagctggt tttacgggga ctggcaagcc
     2101 gaagagaaaa gagaagtaag gcgtttttcg atcgaagacg gggacgagat tatcggaagt
     2161 ctgcaagcca tccgttatcc gcttactttc gacaaaagct acatatatat accttacggt
     2221 ccggtattga aaaacaatct aacggaaaaa accgccgctt ttttgaaaaa atcattgaaa
     2281 gaggttttta acgacggaaa aacggtcttt atccggctgg atttttggcc ggcgctaaaa
     2341 gaccgcttgg aaaaaaccgc cgggaagttt ttcgtcaaat ctccgagaag ctcttatcgc
     2401 ggatcgaatt tccagccacg ggccgaacgg gtgatcgatt tgaagcagcc ggaaaacgag
     2461 atcttcttcg ccatggagcc gaaggcgaga tataacgtca ggctggcatt gaaaaaaggt
     2521 gttgtcgtta aaaccatcac caagaatttc ttggattatt tcgaggattt ttattctttg
     2581 atgacggaaa ccgccaatcg ggatggtttc agccttcatc cccgaagcta ctacgaagaa
     2641 atattcaaca gcgccgaaaa gcgcaaaaac gcgtttttgg ccctggcgtt ttacggagac
     2701 accctgctcg tggcgaacct cgtcgttctt tatggagaaa ccgctttttt tcttttcggg
     2761 tcgtcttcgt ccgaataccg cgaactgatg ccttcttatc tttgccaatg gcaaagcttg
     2821 agaaaagcga aagagctcgg gttttcctat tacagtttcg gaggaataag ccacaaagga
     2881 ctgaacagaa gttgggaggg gatttccgtt tataaaaaga agttcggcgg gtttgtcagg
     2941 gaatattctc ctttttacga tctggccatc caaccgtttt ggtattatct ttatatctta
     3001 agaaaagctt ttttcggccg ataaacggca aattatgaaa tcttttttga gaaaattggt
     3061 accgcagccg atcctcggag cttatcacta ttcgatggcc aagcttggcg ctttggttta
     3121 cggattccca tcgaggaaaa tgcttattat cggcgtcacc ggcaccaagg gaaaaacctc
     3181 gaccgccaat ttcatctggg gcgttctcaa ttcctcgggt tggaaaacag gcattgtcag
     3241 tacggccaac atgaggatcg gagacgagga atatccgaac gtttaccaca tgaccatgcc
     3301 ggggagattc gaaatccaga agtggctcaa aaaaatgctc gaggccgatt gccgggcagc
     3361 ggtcgtcgaa accacttccg aggggattaa ccagtatcgg cacaaaggca tcgaatacga
     3421 ttttctggtt ttcaccaatt tgactccgga acatttacct tcccacggcg gcagtttcga
     3481 aaaatacaag gaagccaaag ggaagatctt cgttgatctc gcgaaaacca aaagaaaagt
     3541 tcttcaagac cttgtcctta agaaaatctt catagtcaat aacgacagcg aatacaaaga
     3601 ctattttttg aatttcgagg cggataaaaa aatcactttc ggaattaggg gacaggcgga
     3661 ctatttggcg gaaaacatca agactttgaa cgaagaaacg agctttgata tcggcccggg
     3721 tcattacgtt atttccattc cggggaattt caacgtctat aacgctttgc ccggaattgt
     3781 catcgggaaa gaactcggtt tctcttccgc aaacatagcc gtcggattga agcagctcaa
     3841 aaacattccc ggccggatgg aaaaaattga cgaaggtcag gatttcgcgg tttttgtcga
     3901 ttacgcccac gagaaagaaa gcctgaccgc agttttggag acggccaaat ccatgtctcc
     3961 gaaccgcatc gtcattcttc tcggagccga aggcggaggc cgggacaaat ccaagcggcc
     4021 ggtgatggga aaactcgccg gagaaaaagc ggattacgtt gtcgtctcga acgtcgaccc
     4081 ttacgaagac gagcctggtc cgatcatcga agatatcgcg aaggcggccg aagaaaccgg
     4141 caaggtccgt gatcaaaatt tgttttctat cgaagaccgt cggacaggca tcagaaaggc
     4201 gctttctctc gcgagaaaag gagatgtggt tttgatcacc ggcaagggag cggagcagtc
     4261 gatcatcatc ggcggcaaaa agtactcctg ggacgataga aaggtcgtca gagaagaatt
     4321 ggaaaaaatt ttaaacaaaa attaaacatg aacaaaatca aaaaaatatt attcgttgtt
     4381 ttgcttatag gaattttctg ttgggttctt tacgactgga gcgattggaa aatagaaatg
     4441 aaccaggaaa aagttttgtc ggacgagaaa aattatacgg aaccggccgg agagagtgaa
     4501 aaacccggag cggccatcga tccccaaacg cttgtttggg aaaaagcttt gtccaacgcc
     4561 ccctggggac cgagggattc gcagggagga attgttttcg acggtaagat gtggatcttc
     4621 ggagggcttc gcggatttcc tgaagccgaa aacaattacg gcgtggcacc gcattttaac
     4681 gacatatgga gttcggaaga cggcgcgaat tggatcctcg aaaaagaaca cgctgcctgg
     4741 tctccgcgaa gatcaatgtc cgtcgaatat ttcaaaggaa agctttggat gttcggcggc
     4801 tggagcacga cgaccggtta tcgcaccggc atctgggttt ctgacgacgc cgtcaattgg
     4861 aaacaggtcc tgaaagacgc gccctggggc ggccgcgaag gccagacctc ggccgtattc
     4921 caaggaaagc tttggatgtt cggcggattg agtttcggcg ccaggctcgg agatcagaac
     4981 ctcaaaaacg atgtctggaa ttctgaagac ggaatccatt ggaatctggt gaccgaaaat
     5041 gccggatggg ctccgcgcta cgatcacgga acagcagtat tccaagacaa attatgggta
     5101 gctggaggcg taggccacgg taaagccatg aacgatgttt ggagttcgag cgacggagtg
     5161 aattgggtct tggaaaacgg gaacgctccg tgggaagaaa ggcatggaat gcctttggtg
     5221 aattacaagg atcgtctttg gctcgtgagc ggttggggaa ccgtgaacga agccggaacc
     5281 caggatgtct ggtattccgc gaacggaaaa gattggcaaa aagccggaat cgatttttcc
     5341 tggcccggcc gggaagacca cgaggtgatg gttttccaag acaaactttg ggtgatggcg
     5401 ggtatgggtc ctttctgggt ttggaacagc gatgtctggt attcggattt cggcagatag
     5461 tcctataatg aatttatgcc gcagatagaa tgcgagattc gcagttttat cgataagcaa
     5521 aaatacgagg agctaatcga gaagttcaaa aaagaagcgg agtttttggg cgagcgggac
     5581 gaaaccactt attatctcga cgtgccgcag gatttgcgga tccagcgaaa cgacgcttac
     5641 gccaagatat ggctcaaaaa aggcaaactt cacgacgacg cgagagagga aatcgaggtc
     5701 cgtttctcga aagaggattt cgggaaaatg gaagaaatgt tgtccgagat cggcatcgac
     5761 ccggaaataa aatggttccg ccaccggaac gaattcttgt ggaacaaaat
//
LOCUS       DAOPQT010000019         5780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_15179_length_5780_cov_3.953537, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000019 DAOPQT010000000
VERSION     DAOPQT010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15179_length_5780_cov_3.953537"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..459)
                     /locus_tag="PKG74_00805"
     CDS             complement(91..459)
                     /locus_tag="PKG74_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89857.1"
                     /translation="MMDWIFYFVRLFFTRIGDFLRHWYVSAFYLIADLTLKFLEWLDH
                     SFALKVTVRYWLHPLYQERNILGYLVGFLVRTVRIIFAVVVYSFVFVFAAIVYLAWAA
                     VPLFLVYKTLEAYGIKRDIF"
     gene            complement(492..1720)
                     /locus_tag="PKG74_00810"
                     /pseudo
     CDS             complement(492..1720)
                     /locus_tag="PKG74_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
     gene            complement(1726..3408)
                     /locus_tag="PKG74_00815"
     CDS             complement(1726..3408)
                     /locus_tag="PKG74_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOB89858.1"
                     /translation="MLFTEIVLAIVLASLLGILMTLLKQPSVLGYILAGAIIGIFGFV
                     GPESLEVISSLASIGIAFLLFMVGLEMNLEKIRHLGKDIILLGTLQTVFVVILGFFLS
                     RFLGFGPIPSIYIAFSLSFSSTIIIVKLLSEKKELNSLYGRLVVGCMIFEDFLAILMI
                     IFLEGVGASAGTGNFGGDFAITVLKGIGLFLGTIFLSRLMPKVLDYFGSKSETLFLFS
                     LAWGLGIAALMDLPIIGLGIVVGGFLAGLSLASSAERYGISGKIRWIRDLFVLLFFVW
                     LGSQMNLNLNGEILLRAGIFSAFVLFVCPIISMFVVSALGYKSRTAFFVGLTAAQISE
                     FSLVVAMKGFELGHLSQNDVSVMVIVAVTTIIGSSYYVMHGEKLYALFKKPLKALEFR
                     KTRESAATGSVEGYKDHIILIGGHRLGENIVKSLIEIGEDFVVIDFDPQIVHSLKDQG
                     LSVIYGDIAEDDVREAAGFQNARLVISTAPLFEDNIVVLDTARRSNPKAQIILRAESD
                     ADAKALYDRGADYVISPYFLGGEYLGKILGEDKSCRKLEAVKKKDIELLSSR"
     gene            complement(3465..3536)
                     /locus_tag="PKG74_00820"
     tRNA            complement(3465..3536)
                     /locus_tag="PKG74_00820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3501..3503),aa:Arg,seq:ccg)
     gene            complement(3578..4702)
                     /locus_tag="PKG74_00825"
     CDS             complement(3578..4702)
                     /locus_tag="PKG74_00825"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HOB89859.1"
                     /translation="MKFLKSYKEVVVPACMLASLIIGAGMFSLPYVTSRSGIFTGLFY
                     LLAFAGIMSLIHVFYGEIVIADKSGRRFASQSRQYLGQTGFLVGAISLVAGTTLALTI
                     YLILTEGFAKLMFPGFSASVILFWILGSVGVIIGIKGIAGLNNLLTLFMVLLAGIVAF
                     FGLKNGTSFLRNVPFWPKGDISLWLLPIGPFLFSLNGRGAIASIEDYFEANRLDIRKM
                     KKAIVWGTLAAAAVYLLFAVGMLGITAGAPARDIMIDLALLPDYLRFLVAGLGLIAVF
                     TSYVFLGLELKMVLEKDFRFRGIAASAAVVVAPIAIYFLANRDLVSLISVAGGVFLGL
                     ESAIVILMWEKLKSKKLWLNRMIVFVFIVGALYEVLKRTI"
     gene            complement(4680..4928)
                     /locus_tag="PKG74_00830"
     CDS             complement(4680..4928)
                     /locus_tag="PKG74_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89860.1"
                     /translation="MEKPVGVVTHYYDKLGVAIIKLSDGLKIGDQIRILGKTTDFSEE
                     NTSMQIDHRSIESASKGEEIGIKIGREAREGDEVFKIV"
     gene            complement(4951..5535)
                     /locus_tag="PKG74_00835"
     CDS             complement(4951..5535)
                     /locus_tag="PKG74_00835"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89861.1"
                     /translation="MPEPILKYKICVSGAAETSHCGDEAFGQAEELGREIIRHGAVLV
                     DGATTGFPFWAAKGAKLEGGTVVGVSPAGSKQEHIKVFKLPVDYHDIIMYTGHGYSGR
                     NLLLTRMSDAVVVGCGRMGTLNEFTIAFEDKKPIGILEGKWVTDEVIKTIIEKSFRGE
                     EMKDRIVYDSDPKILIEKLISIVSRLEEENGINK"
     gene            complement(5556..>5780)
                     /locus_tag="PKG74_00840"
     CDS             complement(5556..>5780)
                     /locus_tag="PKG74_00840"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HOB89862.1"
                     /translation="EEVPVRCRVRSIDGYSSHADQEGLIRWLRPMKDSLKKMFVVHGE
                     LEASIKFASMASDRLAVPSEVPSKGESVIL"
BASE COUNT         1553 a         1519 c         1413 g         1295 t
ORIGIN      
        1 aaagcggcgg cggagaaaac caaggtgatc gcgaaacgga aaaccttgac caggctttgg
       61 ccgatgacac taagcctcaa aaacggctcg ttaaaatata tctctcttga ttccataggc
      121 ttctaaagtt ttataaacca aaaacaaagg gacggccgcc caagcgagat aaacgatcgc
      181 ggcgaaaaca aagacaaagg agtaaacaac gaccgcgaaa attatcctca cggttctcac
      241 caaaaaaccg accaaatatc cgagaatatt tctttcctga taaaggggat ggagccagta
      301 gcggaccgtg actttcaagg cgaaggaatg gtcgagccat tcgagaaact tgagcgtcaa
      361 atcggcaata aggtaaaaag cggaaacata ccaatgcctc aaaaaatctc cgatccttgt
      421 gaaaaacagc cggacaaaat aaaaaatcca atccatcatg gcttcagtat accccgaaaa
      481 ctccgccgag gctaaaaaag agaattcgcc ttttcttctt tgaaaatctt gattttcccg
      541 tactcgccgt cgtagcccgg gtcaatttcc acttctcccc gcctcacttt gacgatgcct
      601 tcggcgatct tcggatcgat cgaagagaaa agttcggctt cggacttgtc catcagaacc
      661 tcgagctcgg gaacgacgga gacgatccgc tcgtattcct ctttcacttt tttggaagaa
      721 gttcccactc cgtaagcttc ggcgatgatc tcgtcgagcg gtatcaaact cttatacgga
      781 atcttttttt cgtcgacgta tccgttcggc cggtcggcca gcttgtgaac gcggttcaaa
      841 acgccgatgg tcaagggttt gccgcagacc gggcatttgc cgccgatttt caatgtttct
      901 tccggagaca aggaaacgcc gcaattccga tggccgtcga aatggtattt cccttcttcc
      961 gggaaaaatt cgatggtctc caagaacttg gagcaatcgc ggcttttgat agcgtccatg
     1021 atcccttgat aagaaagctc ggcgtcgaaa acattggctt cccggccgag gcgctggaga
     1081 gaatgcgaat ccgaattgga aatcaaagaa atgttgtcga gtttggaaag cctccaattc
     1141 atggccggat cgctcgaaag tccggtttcg atggcgaaaa tattcttggt ctcttccccg
     1201 aaagcctctt cgagcgaatc gaacccggac attgacccga aaaccgagaa ccaaggagtc
     1261 caggcgtggg ccggaacaaa ggccgcctcg ggattcgctc cgaggaccat tttcagaagc
     1321 tcttcggaat cgagcccgat gatcggccgg ccgtcggaaa taagattggc gccgcgcctg
     1381 ataagactct cgtttatttt ccggaccgtg gccgtatccg gagcgaaaac aaggttgtga
     1441 acccgacgcg tggctccgcc cctcgaatag atgttcgaga tttcggtcgt gagcaaaaac
     1501 ctggttccgc tgtctttgga tttaagcttg aaaagcccgg gttcggccgg aacaagaagg
     1561 ctttcgatgt cggcgaacca gcgggggtgg gtaaaatcgc ccgtgccgat gaccttgatg
     1621 cttttttctt tccccaaagg tcgaggttgt ccagagtcat gtcccgcgaa accgcccggg
     1681 aaaaagaaga atgtatgtgt aaatccgcta tgaatcgcat ttgttttaac ggctgctcaa
     1741 aagctcgatg tcttttttct tgaccgcctc gagcttacgg caggatttgt cttctccgag
     1801 tattttgccc aaatattctc cgcccaaaaa atagggcgag atcacgtaat cggcgccgcg
     1861 gtcgtacaag gctttggcgt cggcgtcgct ctccgctctc aagatgatct gggctttggg
     1921 gttgctccgc ctggctgtat cgagaacaac gatattgtcc tcgaataaag gagcggttga
     1981 aatgacgagc ctcgcgttct ggaatccggc cgcttccctc acgtcgtcct cggcgatatc
     2041 tccgtagatg acactgagcc cttggtcttt caaagagtga acgatctgcg gatcaaagtc
     2101 gatgaccacg aaatcctcgc cgatctcgat caaagacttg acgatattct ctcccaaacg
     2161 gtggcctccg atcaaaataa tatggtcctt atacccttcg acggatccgg tggccgcgga
     2221 ttcgcgagtt ttcctgaact ccaatgcttt caagggcttt ttgaacaaag cgtaaagctt
     2281 ttctccgtgc atcacgtaat aggatgaacc gatgatggtg gtgacggcga cgattaccat
     2341 gacgcttacg tcgttctgac ttaagtgccc caattcgaat cccttcatcg ccaccaccag
     2401 gctgaactcg gaaatttggg ccgcggtcaa acccacgaaa aaggccgttc gactcttgta
     2461 accgagggcc gagacaacaa acatggaaat gatcggacag acaaagagaa caaaagcgga
     2521 aaagattccg gctcgcaaga gtatttctcc gttcaggttc aagttcatct ggcttccgag
     2581 ccagacaaag aaaagaagaa cgaacaaatc cctgatccag cggatttttc cggagattcc
     2641 gtagcgttcg gccgagctgg cgaggctaag cccggccaaa aacccgccga cgacgattcc
     2701 gagaccgatt atcggcagat ccatcaaggc ggcgatgccc aaaccccaag ccaaactgaa
     2761 aagaaaaagc gtctcgcttt tggaaccgaa ataatccaaa actttgggca taagcctcga
     2821 aagaaagatg gttccgagaa aaaggccgat tcccttcaaa acagtgatgg caaagtctcc
     2881 accgaaattc cctgttccgg ccgaagctcc cacgccttcg agaaaaatga tcatgaggat
     2941 ggccaaaaag tcttcgaaaa tcatgcagcc gacgacgagc cggccataaa ggctgttcag
     3001 ctcttttttc tccgaaagca gtttgacgat gatgatggtc gaagaaaaag acaggctgaa
     3061 agcgatataa atcgacggga tcgggccgaa tccgagaaac cgactcaaga aaaaaccgag
     3121 aataacgaca aaaacggtct gtagtgttcc gagaagaatg atgtcttttc cgaggtgcct
     3181 gatcttttcc aggttcattt cgagcccgac catgaataga aggaaggcga tgccgatcga
     3241 agcgagcgaa gagatgacct cgagcgattc cggcccgaca aacccgaaaa tgccgatgat
     3301 cgctccggcc agaatatacc ccaaaaccga aggctgtttt agaagcgtca tcaggattcc
     3361 gaggagcgaa gcgagaacaa tcgccaagac tatttccgta aaaagcatag gttaagtata
     3421 gataagaaaa atagtttagg gaacacgaaa ccggtatttt attgtgcctc gggagggaat
     3481 cgaaccttca tcgcaggctt cggaggcccg cattctatcc attgaactac cgaggcggaa
     3541 caaaaggatt atacggccag ttcaaaaaac cggcaattca gatcgttctt ttaaggacct
     3601 cgtacaaagc tccgacaata aacacaaaaa cgatcatccg attcagccag agtttcttgg
     3661 atttcaactt ctcccacatc agaatgacga tcgcgctctc gaggccgagg aaaacgccgc
     3721 cggccacgga aatcagactg acgaggtcgc ggttcgccaa aaaatagatc gcgatcggcg
     3781 caacgacaac cgcggccgaa gccgcaattc cgcggaagcg gaaatctttt tccaaaacca
     3841 ttttcaattc gagtccgaga aaaacataag aagtgaaaac agcgataagc ccgagtccgg
     3901 cgacgagaaa tctgagataa tccggcaaaa gagcgagatc gatcataatg tcccttgccg
     3961 gagcaccggc ggtgatcccc aacatgccga cggcaaaaag caaatagacc gcggccgcgg
     4021 ccagggttcc ccaaacaatc gcttttttca tttttctgat atccagacga ttggcttcga
     4081 aatagtcctc gatcgaggcg atggccccgc ggccgttcaa ggaaaaaaga aaaggtccga
     4141 tcggcaaaag ccagagcgat atgtcgcctt ttggccaaaa aggaacgttt cgtaaaaaac
     4201 tcgtgccgtt tttcaatccg aaaaaggcca ctattcccgc gagcaagacc atgaaaagcg
     4261 tcagtagatt gttcaacccg gcgattcctt tgatgccgat gatgacgccc acgcttccca
     4321 aaatccaaaa caaaatcacg gaagcggaaa aaccaggaaa catcagtttc gcgaagcctt
     4381 cggttaagat tagataaatc gtcagggcta aggttgtccc ggcaaccaaa gaaatcgctc
     4441 cgaccagaaa tccggtttga cccaaatatt gccggctttg gctcgcgaaa cgacggccgg
     4501 atttgtccgc gatcacgatc tctccgtaaa aaacatggat caaagacatg atcccggcga
     4561 acgccaaaag ataaaaaagc ccggtgaaaa taccggaacg actcgtcacg tacggcaaag
     4621 aaaacatgcc ggcgccgatg atcaaagacg cgagcatgca cgccggaacg acgacttctt
     4681 tatacgattt taaaaacttc atcgccctcg cgcgcttccc tgccgatttt gatcccgatt
     4741 tcttcgcctt tgctcgccga ttcgatcgac cggtggtcta tctgcatcga agtgttctct
     4801 tcggaaaagt ccgttgtttt tccgaggatc ctgatctggt cgcctatttt cagtccgtcg
     4861 gaaagtttga ttatggccac gccaagtttg tcgtaataat gggtgaccac gcccactggt
     4921 ttttccattt gttaaattga attccgacct ttatttgttt atgccgttct cctcttccag
     4981 cctggagacg atcgagataa gtttctcgat caggattttc gggtccgaat cgtaaacaat
     5041 gcggtctttc atctcctctc cgcggaagga tttttcgatg atggtcttga tgacttcgtc
     5101 cgtcacccat tttccttcca ggatgccgat cggcttcttg tcctcaaaag caatggtgaa
     5161 ttcgttcaag gttcccatgc ggccgcagcc gacgaccacg gcgtcggaca tccgcgtcag
     5221 caacaggttg cgaccggaat atccgtggcc ggtgtacatg atgatgtcgt gataatcaac
     5281 cggaagcttg aaaaccttta tgtgctcctg cttggatccg gccggagaaa ctccgacaac
     5341 cgttcctccc tcgagcttgg cgcctttggc cgcccaaaaa ggaaatccgg tggtggcgcc
     5401 gtcgacgagg accgcgccgt gcctgattat ttcccggccg agctcttcgg cctgtccgaa
     5461 agcctcgtca ccgcaatggg aagtctcggc cgctccggaa acgcagattt tatatttaag
     5521 tatcggttcg ggcatgttgt tctttgttgt tttctttata atatcacact ctcgcctttg
     5581 ctcggcactt ccgagggaac cgccagccgg tccgaggcca tcgaagcgaa tttgatgctc
     5641 gcttcaagct cgccgtggac gacgaacatc tttttgaggc tgtctttcat cggccggagc
     5701 catctaatca atccttcttg gtcggcatgc gaagaatagc cgtcgatcga acgcacccgg
     5761 cagcgcaccg gcacttcctc
//
LOCUS       DAOPQT010000020         5700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_15492_length_5700_cov_2.672631, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000020 DAOPQT010000000
VERSION     DAOPQT010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15492_length_5700_cov_2.672631"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2007
                     /locus_tag="PKG74_00845"
     CDS             <1..2007
                     /locus_tag="PKG74_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89863.1"
                     /translation="VWLSTALIDGAIALNETIYGPNSLAKAGFDVVLQFVNIFFVFGI
                     LTTGFATMLRPVFPNFGLLNKIGKERLPQLLLAFFLVNFAFTLAGAIISLSDNITRSL
                     NQMISSSNAWEYFGALPMDGMGNIESAFGLFVVPILTALVGVGIILTMFAIAIMFFIR
                     WAYLTFLVIISPFVIFMEVFPVITFKNLGSFKNWSEQFVRWLLFAPVMILFIYIILSL
                     SSAGLDTTANTVFGASLGLYVGKMILQLILLVIGLQWANSLSLGGGKVVFGTANTLLN
                     RAKNFPIREGKTLGTILGQRVTDTPQYKQVRSTLAGSFITRPLAVQMDVATARLKTGQ
                     EKSIKEYGENYKDWSNETLIRYLDSANLSLMNPVAQTALLQQLQNRKIAVLKEEDVTK
                     AGLDKNRLAYLNGQIGRLIGSARGIKATDAFKKTHPHEISDYKTPKDPKEIEKAAEKI
                     TKAVRGLKPSEILDVVPEAFQEWPVVLGLNESHFRKLGDEGSDEQRTKIEDTLKSLVS
                     DPTKTNKIADIVKRLRRIPSWAEFGEELNKMLSASATPPKTTPPARPTSSTIITPGPQ
                     PKPGANIITPGPQPNPQARIITPGAQPQPKATVVTPGPSPKPGAKIVTPGPQPNPEAG
                     IINPYRKQPDVKINIGKEALEEAARRRFLDTNPPPADSGNTSPK"
     gene            2062..2880
                     /locus_tag="PKG74_00850"
     CDS             2062..2880
                     /locus_tag="PKG74_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89864.1"
                     /translation="MAEIYEELAEKFGIDVSAITLTIYYLESKKISPADFTIELQKRS
                     GKTASPELIKQIIDRVFAPIKNDLLEFGIDIDLIQVPADSTMIASVPPTPTIPSAAAS
                     APTISTPTPTPAPASQPVPPASTPYILHQEAAFKTLSEEHDINYEPIKRAFFSSSQPP
                     APTFKEPTGPAKIQFSGQQPEAPVTPKNQEVARTEAFVPKIVHYSTLMTPLSPFGNSK
                     GTIAPQPTEPPAPAAATPIPEPKLPQTKFNDEIIEEVAKSAASPEKIDLKDLPL"
     gene            2920..3420
                     /locus_tag="PKG74_00855"
     CDS             2920..3420
                     /locus_tag="PKG74_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HOB89865.1"
                     /translation="MQFLVPQFVSIENRIIGPLTLNQFIYLAIPAVISYFLYFPLANW
                     LWIVIAGILMGIGSALAFAKINTRPLPAVIKAAAYYLIRPKAYVWPAYSEEKIVAVTA
                     VRAPAKPSLTTLFERITTSISPVPKREMSFEADFIRNQNEWRQRYAIIQKITGEKESV
                     RRIDYR"
     gene            3424..>5700
                     /locus_tag="PKG74_00860"
     CDS             3424..>5700
                     /locus_tag="PKG74_00860"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOB89866.1"
                     /translation="MARETKNISSESILPVKKIEGNSVFLKDGGLRKVIMVNGVNFDL
                     KSSEEQQMILAAFADLLNSLDYPIEISVSSRKFFIDDYLNRLKEKEEQEQNEMMRDQI
                     AIYREFIDTFVSENAVMKKGFFITIPHEIISLPGVGKKPEPGAEPKAVRDAEERLNLK
                     VDQVIRGLHRLGLRGIVLEEAELRELFYGAFNPGEKLNPAEAQKFIGPRRFEISPSSL
                     KVNNLVAKTLFISGYPRYLSSGWMSDIINLAELADISIHVSPTETRDVLRQLRNRSAQ
                     VQVQIMDLEEKGLVRDPMLETALYDIEALRDNLQQAQEKLFSVGVYITIFGKDASDLN
                     RIFDRISSGLESKLIYAKPLLFENLQGFYSTLPIAEDRLEIINPFNTNTLSSFFPFVS
                     ATLSSDEGVLYGINRHNNTPIIFDRFSLENANTVVFAKAGSGKSYATKLEVLRSLMVG
                     DEVIIIDPENEYQNLAESVGGSFIKISLDSPNHINPFDVPPIPKDESPSEILKSHIVN
                     LTSLMKLMFGEIKPEEEAVIDQAITEAYASRDITPDKDFSGSTPPLLEDLEEIFRNIE
                     GGRTMADRLYRFTKGSYAGFLNKPSNIDTANRLVVFSIRDLEDELRPIAMFIILNFVW
                     NLVRADFKKRLMIIDEAWWLMRYPDSASFLFGLVKRARKYYLGITTITQDVEDFLSSS
                     FGRPIITNSSLQLLLKQSPSSIEPTAKAFGLSEAEKNYLLEVDIGQGLFLAGMTHAAI
                     EVVASPFEHQIITTNPEEI"
BASE COUNT         1519 a         1563 c         1291 g         1327 t
ORIGIN      
        1 gtctggttgt ccaccgcatt aatagacgga gccatcgcct tgaacgaaac catttacggg
       61 cccaatagtc tggccaaggc gggatttgac gtcgtgcttc aattcgttaa tatattcttc
      121 gttttcggga tactcaccac tggcttcgcc accatgctga gaccggtttt ccccaatttc
      181 gggcttttga ataaaatcgg caaagaaaga ttgccgcaac tcctcctcgc ctttttcctc
      241 gtcaacttcg ctttcacttt ggccggagcg attatcagtc tttccgacaa catcactcga
      301 tctttaaacc aaatgatctc ctcgtccaac gcctgggaat acttcggggc acttcctatg
      361 gatggtatgg gaaatataga aagtgctttt gggctctttg ttgtgccgat tttgaccgct
      421 cttgtcggcg tcggaatcat cctcacgatg ttcgccatcg ccatcatgtt tttcatccgc
      481 tgggcttatc tgacttttct cgtcatcatt tctcctttcg tgattttcat ggaggttttt
      541 ccggtcatca ctttcaaaaa tctcggctcg ttcaagaact ggagcgagca attcgtccgc
      601 tggttgcttt tcgcgccagt gatgatcctt ttcatttata tcattctgag cttaagctcc
      661 gccggcctcg acaccaccgc caatacggtt ttcggagcct cgctcggatt atatgtcggt
      721 aaaatgattc ttcaattgat tttgctcgtt atcggattgc aatgggcgaa tagtttgagt
      781 ctcggcggag gtaaagtcgt ttttggaaca gcgaacacat tgctgaacag ggccaagaat
      841 ttcccgatca gagaaggcaa aaccctaggc acgattttag ggcaaagagt cacggatacg
      901 ccgcaatata aacaagttag aagcactctg gcgggaagct ttatcaccag acctttggcg
      961 gtgcaaatgg atgttgcaac cgccagatta aaaacgggcc aagaaaagtc cattaaagaa
     1021 tacggcgaaa attataaaga ctggtctaac gagactttaa ttcgttacct agactccgcc
     1081 aacctttctt tgatgaatcc ggtggcgcag actgcgctgt tgcaacagtt acagaacaga
     1141 aaaatagctg ttctcaaaga agaggatgtg actaaagccg gattagataa aaatcgcctg
     1201 gcatatctta atggacaaat tggaagatta atcggatcag ccaggggaat caaagcaacc
     1261 gacgccttta agaaaactca ccctcacgaa atctccgatt acaagactcc taaagacccc
     1321 aaagaaattg aaaaagctgc cgaaaaaata actaaagccg ttaggggcct taaaccttcg
     1381 gaaatcttgg atgtagtccc cgaagcgttc caagagtggc ctgtcgtctt gggccttaac
     1441 gaatcccact tcaggaagct cggcgatgaa ggaagcgatg aacaaaggac aaagatcgaa
     1501 gacacattaa aatcgttggt atccgatccg acaaaaacaa ataaaatagc agatatcgtc
     1561 aaaagattaa gacgcattcc atcttgggcg gagttcggcg aagaactgaa taagatgctt
     1621 tctgcttcgg ccacgccgcc caaaacgaca cccccggcgc gcccgacttc gtcgacgatt
     1681 attacgcctg ggccgcaacc gaaaccaggg gcaaacatta ttactcccgg acctcaaccc
     1741 aacccacaag cccgcattat aacgcccggg gcgcaaccac agccaaaagc gactgttgtt
     1801 actcctggtc cgtcgcctaa accgggagcg aaaatcgtca cgcctggacc acaacctaac
     1861 ccggaggcgg gaatcataaa tccttaccgc aaacaacccg atgtaaaaat caatatcggg
     1921 aaagaagcct tggaggaggc ggccagacgc cggttcttag acaccaatcc accgccggcc
     1981 gattcgggaa atacaagtcc gaaataaaac gcgacgatgg aaatcccgct tattatcaag
     2041 tcttggttta attccgagcc gatggccgaa atctatgagg agttggccga aaaattcggt
     2101 atcgatgttt ccgccataac cctgactatt tactatttgg agtccaaaaa aatatctccg
     2161 gcagatttta cgatcgaact tcaaaaacgc agcggcaaga cagcttctcc ggaattgata
     2221 aagcaaatca tcgaccgggt tttcgcgcct attaaaaacg atcttttgga attcggaata
     2281 gatatcgatt tgattcaagt tccggctgac tcgacgatga tcgccagcgt tccgccgaca
     2341 ccgactattc cgtcggcagc ggcttcggct cccacaatct cgactccgac cccaaccccg
     2401 gctccggcgt ctcagcctgt tcctccggcc tcgactcctt atattctcca ccaggaagcg
     2461 gctttcaaga cgctttcgga agaacacgac atcaattacg aaccgatcaa acgggctttt
     2521 ttcagctcaa gtcagcctcc ggcgccgaca ttcaaagaac cgaccggtcc ggccaagatc
     2581 caattcagcg ggcaacaacc ggaagctccg gttactccca aaaaccaaga agtggcgaga
     2641 accgaggctt ttgtcccgaa aatcgtgcac tattcgacgc tcatgacgcc tttaagccct
     2701 ttcgggaaca gtaaaggaac aatcgctccg cagccgaccg agccgccggc tccggcggcc
     2761 gcgactccga tacccgaacc aaaactaccg cagacaaaat tcaacgacga gatcatcgaa
     2821 gaagtggcca aatccgcggc ttctccggaa aaaatagact taaaagacct tcctttgtag
     2881 aattaactaa gcccttcttt gattggaaag gcgttaacga tgcaattttt agttccgcaa
     2941 tttgtttcga tcgagaaccg catcatcggt cccctgacac tgaaccagtt catctatctg
     3001 gctattccgg cggtcatctc gtattttttg tattttcctc ttgccaattg gctttggata
     3061 gtcatcgccg gaatcctgat gggaatcggg tcggctctcg ctttcgccaa gatcaacacc
     3121 cggccgcttc ccgcggtgat caaagccgcc gcctattatc ttatccggcc caaggcttat
     3181 gtctggccgg cttattccga agaaaagatc gtcgccgtca ccgcggtcag ggctccggcc
     3241 aagccgtctt tgaccactct tttcgaacgc attacgacga gtatttctcc ggtgcccaaa
     3301 cgggaaatgt cctttgaggc ggactttatc agaaaccaaa acgaatggcg gcaaagatac
     3361 gccatcatcc agaaaatcac cggcgaaaaa gaatccgttc gccggatcga ttacagataa
     3421 tccatggcca gagaaaccaa aaacatctct tcggaaagca ttctgccggt caaaaaaatc
     3481 gaaggcaatt cggttttcct gaaagacggc ggactccgca aagtcatcat ggtgaacgga
     3541 gtcaatttcg atttgaaatc gagcgaagag cagcaaatga tccttgctgc cttcgccgac
     3601 cttttgaatt cgctcgatta tccgatcgaa atcagcgtct cctcgagaaa attcttcatc
     3661 gacgactact tgaaccgttt gaaagaaaaa gaagagcagg agcaaaacga aatgatgcgg
     3721 gatcagatcg ccatctaccg ggaattcatc gatacttttg tttccgaaaa cgccgtcatg
     3781 aaaaaagggt ttttcatcac catcccccac gaaatcatca gcctgcccgg agtgggtaaa
     3841 aaacccgaac cgggagccga gcccaaggct gtccgcgacg ccgaagaaag attgaatctc
     3901 aaagtcgacc aagtcatcag aggactccac cgcctcgggc ttcggggaat cgttctcgaa
     3961 gaagccgagc tccgggagct tttttacggc gctttcaatc cgggagaaaa actgaatccg
     4021 gccgaagccc aaaaattcat cggcccccgg agattcgaga tctccccaag ctcgctcaag
     4081 gtgaacaacc tcgtggccaa gaccttattt atctccggat atccgcgcta tctttctagc
     4141 ggctggatgt cggacatcat caatctcgcc gaactggccg acatctccat ccatgtttcc
     4201 cccacggaaa cccgggatgt gctgcggcaa ttgcgcaacc gatcggctca agtgcaggtg
     4261 caaataatgg atcttgagga aaaaggcttg gtccgggatc cgatgctcga aaccgcgctc
     4321 tacgacatcg aggctttgcg cgacaacctc cagcaagccc aggaaaagct tttttccgtc
     4381 ggcgtctaca ttaccatttt cgggaaagac gcttcggatc tgaaccgtat tttcgatcgc
     4441 atctcgagcg gcctcgaatc caaactcatc tacgctaagc ccttgctttt cgagaacctc
     4501 cagggattct attccaccct gcctatagcc gaagatcgtt tggaaatcat aaatcccttc
     4561 aacaccaata ccctttcctc gtttttcccg tttgtttccg ccactctctc ttcggacgaa
     4621 ggcgtgctct acggcatcaa ccgccataac aacacgccga tcattttcga ccggttttct
     4681 ttggaaaacg ccaacaccgt tgtcttcgcc aaagccggct cgggaaaatc ctacgccacc
     4741 aaactcgagg ttttgagatc tctcatggtc ggcgacgagg tgatcatcat tgatccggaa
     4801 aacgaatatc agaatttagc cgaatcggtc ggcggatcgt tcatcaagat ctcgctcgat
     4861 tccccaaacc acatcaatcc tttcgatgtt ccgccgattc cgaaagacga aagcccgagc
     4921 gaaatcctga aatcccacat cgtcaatctg acgagcctga tgaagctcat gttcggagaa
     4981 atcaaaccgg aagaagaagc ggttattgac caggccatca ccgaggccta tgcctcgaga
     5041 gacatcacgc cagacaagga tttttccggg agcacgccgc cccttctcga agacttggaa
     5101 gagatatttc ggaacatcga aggtggccgg accatggccg accggctcta ccgcttcacc
     5161 aaaggaagct acgccggatt cttgaacaag ccctcgaaca tcgacaccgc gaaccgactg
     5221 gttgttttct ccatccgcga cctcgaagac gagctccggc cgatcgccat gtttattatt
     5281 ttgaatttcg tttggaattt ggtgcgggcg gatttcaaaa aaagactgat gatcatcgac
     5341 gaggcctggt ggctgatgcg gtatccggat tcggcttctt ttcttttcgg attggtgaaa
     5401 agagcaagaa aatattatct cggcatcacc accatcaccc aggacgtcga ggacttcttg
     5461 agctcgtctt tcggccgacc gatcatcacc aactcttctc ttcagcttct tttgaaacaa
     5521 tcgccgtcgt cgatagagcc gacggccaag gctttcgggc tttccgaagc ggagaaaaac
     5581 tatcttctcg aagtggatat cggtcaagga ttgttcctcg ccggcatgac ccacgccgcg
     5641 attgaagtcg tcgcttcgcc tttcgagcat cagataatca ccaccaaccc ggaagaaata
//
LOCUS       DAOPQT010000021         5665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_15622_length_5665_cov_3.113547, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000021 DAOPQT010000000
VERSION     DAOPQT010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15622_length_5665_cov_3.113547"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKG74_00865"
     CDS             complement(<1..514)
                     /locus_tag="PKG74_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOB89867.1"
                     /translation="MDYRQKMNELLLATARSNASDLHLAVGRRPMMRIDGSLINMPKE
                     QILTPEMTAGLIGALLTDEQKEKFLTYRDLDLSYAFEDKARFRVNVFFQRGFMAASLR
                     LIPAQIKTLEELHLPSILHKFTGLSQGFVLVVGPAGHGKSSTLAALVDEINHKRMDHI
                     ITIEDPIEYLF"
     gene            complement(561..2294)
                     /locus_tag="PKG74_00870"
     CDS             complement(561..2294)
                     /locus_tag="PKG74_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOB89868.1"
                     /translation="MDSQVLTAALVKKNLLTKEAGEQILKESAVSQKPVEDLLYLRRT
                     VPEEKIAEVKSEIIGIPYQKINVGSLTDDLLALVPKETVQAYKVAPISRTKDMLVVGM
                     VDPQNSRAQDALRFIAKANRLNLGVFIITPADLELVLRRYNPYKSEVEAAIKSLGIKS
                     GDGLSSLQRPVALEEAKGVAEEAPVIKLVASTFREAVERKASDIHVEPMRNRVRVRFR
                     MDGDLEEVASLPIELQQPLVSRIKILSDLKIDETRIPQDGRFRTVIFGRDIDFRVATF
                     PTPMGEKVALRVLDPSIGLKGLDNLGIVGKNADIIQEGIRKPFGMVLVTGPTGSGKTT
                     TLYALLKILNQEDVNIVSLEDPVEYFVEGINQSQVKPEIGYDFASGLRQILRQDPDII
                     MVGEIRDNETAGLAVNAALTGHIVLSTLHTNNAAGVIPRLIDMKVPPFLLPSALNLMM
                     SQRLLGRLCQQCRETEIASPEIQKIIKEEIAKLPEDVRSKLIYKEPYQIFHSPGCPAC
                     KGKGTSGRIAIFEIMKMTRELEKIVAGNPTEADVVDEGERQGMITLRQDGILKVLDGL
                     VSIEEVLRETE"
     gene            complement(2296..2598)
                     /locus_tag="PKG74_00875"
     CDS             complement(2296..2598)
                     /locus_tag="PKG74_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89869.1"
                     /translation="MAVIIQPNQRGSRTFIIISVAAALAILAIGAYYLFFTPAPLIEA
                     ILPANLQSVADLSGLQFNPQEVLQDEFFSSEDPGRSINLPVIGPNSLGRENPFAAF"
     gene            complement(2600..3160)
                     /gene="pilO"
                     /locus_tag="PKG74_00880"
     CDS             complement(2600..3160)
                     /gene="pilO"
                     /locus_tag="PKG74_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOB89870.1"
                     /translation="MAFSFKRFLSILGGIALLVAAVFVFTNFIRPEFEGDNGVKQLRA
                     DRDAAAVVLRMAEAQVEAFANLAQQYEDSFQYKDDLDRSLPLNDDIPHVLAQIYGLAS
                     TNQMVVASVNFQHPPMQSNGSPLVSAYGVLRATVRAEGSYDGLKAFIGDVENNMRLMD
                     IVSMNISASGRANIYSLQLIINTYYQ"
     gene            complement(3144..3689)
                     /locus_tag="PKG74_00885"
     CDS             complement(3144..3689)
                     /locus_tag="PKG74_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89871.1"
                     /translation="MPPQYPTNIDQRFNVGREPAGWPTRLLIFSLFILILLGVSYFGL
                     AYGYKPFLLSDTESIQAQIDALNEQVSPQDRQTFIDFYSQIENLKTLISRHVFSSNIF
                     SFLETNTRQDISYSSMDLDIPSLTANLGGTASTYESLASQLDVFQNLQEVESVSIGGA
                     TLGQTGVSFQMQIIFNNGFFL"
     gene            complement(3693..4727)
                     /gene="pilM"
                     /locus_tag="PKG74_00890"
     CDS             complement(3693..4727)
                     /gene="pilM"
                     /locus_tag="PKG74_00890"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOB89872.1"
                     /translation="MAILDFLKPLKPLLSFQSAIGVDIGTTSIKLVELAIVADRPHFK
                     NYGLLETYSHLSRQNSAIQTSSLKMDDKEAASMLQTLVREVKPGAAAVFASLPPFSAF
                     TTLLELPMMPKNEMDQIMSYQARALVPAAIASVATEWSVVGEFEDVEKYKSVFQAAGL
                     NLRVIEIEGMSLARVFTKGDAELSLILDIGGRSTSLYVARDGHLLSSAYLDYAGGSMS
                     QAIANGLNINVKRAEELKKRRGLLGMGGEYELSTLMLPYVDVILNEVRRVKDNFEKNY
                     RGQIGKVILSGGGGELAGLDKYAADFLQMKVERPSPFSALSYAPTLQPVVNELGGTLA
                     VAIGLGLRQF"
     gene            4743..5441
                     /locus_tag="PKG74_00895"
     CDS             4743..5441
                     /locus_tag="PKG74_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOB89873.1"
                     /translation="MIARMPSFTNIKNQLLDLVFPKLCLNCRCLLEKPIDKENLLCGR
                     CFGSIVVNRLIFPEPGFFLGSATLYRIDAARNLVHFLKYEDFPGVKHPIAKIIEAYLR
                     KSAFIHLFRNVKPEIVPIPLHFMKSISRGFNQAEDIAEVLASGTGWPINTRLLLRTKN
                     TQPQAKLEQKSERMENMAGAFRIKNGLEEYVSGKSFVVVDDVYTSGATMSEAIKILKS
                     GGAKSVAGFVFAKG"
     gene            complement(5444..>5665)
                     /locus_tag="PKG74_00900"
     CDS             complement(5444..>5665)
                     /locus_tag="PKG74_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89874.1"
                     /translation="SDCSSGKFCNWIFICESCSPGSWSCTCNDGYTPSGSLGAGVKCQ
                     YVGGPETCLSRCSSHGGWSGSWSCVDGPC"
BASE COUNT         1334 a         1483 c         1431 g         1417 t
ORIGIN      
        1 caaataaata ctcgatcggg tcctcgatcg taatgatatg gtccatcctc ttgtggttga
       61 tctcgtcgac caaagccgcc aaggtgcttg attttccgtg tccggccgga ccgacgacca
      121 agacaaatcc ctgggaaaga ccggtgaatt tatggaggat cgacggcaga tgcagctcct
      181 cgagagtttt gatctgcgcc gggatcaaac ggagcgaagc ggccatgaat ccgcgctgga
      241 aaaaaacatt gacgcggaaa cgggccttgt cctcgaaagc gtaagaaaga tcgaggtcgc
      301 ggtaagtcag gaatttttct ttttgttcgt cggtaagtag ggcgccgatc aggccggcgg
      361 tcatttccgg agtcaagatc tgctctttcg gcatgttgat caggctgccg tcgatgcgca
      421 tcatcggcct ccggccgacc gctaagtgca gatccgaagc attgctccgg gcggtcgcga
      481 gaagcaattc gttcattttt tgcctgtaat ccatgatgtt tgttttattc ctcggaagtt
      541 gatttttatt ataacacgcc ctattccgtc tccctcaaaa cttcttcgat cgagaccaat
      601 ccgtccagca ccttcaatat gccgtcctgc ctcaaagtga tcattccctg gcgctcgcct
      661 tcgtcgacga catcggcttc ggtcggattg ccggcgacga tcttttcgag ctccctcgtc
      721 attttcatga tctcgaaaat ggcgatgcgg ccggaggttc ccttcccctt gcaggccggg
      781 catcccggag aatggaaaat ctgataaggt tctttgtaaa tcaatttcga tctgacgtct
      841 tccggaagct tggcgatctc ttctttgatg atcttttgga tttccggaga agcgatttcg
      901 gtttcgcggc actgttggca aagccgtccc aaaagccttt gggacatcat caggttcaaa
      961 gccgacggca gaagaaaagg aggaactttc atgtcgatca gcctcggaat gactccggcg
     1021 gcgttattcg tatgaagcgt ggacaagacg atatgtccgg tcaaagcggc gtttaccgcg
     1081 aggcccgcgg tttcgttatc gcggatctca ccgaccatga ttatgtccgg gtcttgtctc
     1141 aatatctggc gaaggccgga ggcgaaatca tatccgattt ccggcttcac ctgcgattga
     1201 ttgatccctt cgacaaaata ctcgaccggg tcttccaagc tgacgatatt cacgtcctcc
     1261 tggttcaaaa tcttcaaaag cgcgtaaagg gtggttgttt ttccggatcc ggtcggtccg
     1321 gtgacgagga ccatcccgaa aggcttcctg attccctctt ggatgatgtc ggcattcttg
     1381 ccgacgattc ccaggttatc gaggcctttc aggccgatgc tcggatcgag aacgcggagc
     1441 gccacttttt cgcccatggg cgtcgggaaa gtggcgacgc ggaaatcgat gtcccgcccg
     1501 aaaatcaccg tccggaaacg accgtcctgc ggaattcttg tttcgtctat tttcagatcg
     1561 gaaaggatct tgatcctcga aacgagcggc tgctggagtt ctatgggcaa agaagccact
     1621 tcttccaaat caccgtccat cctgaatctg acgcgaaccc tgtttctcat cggttccacg
     1681 tggatatccg aagctttgcg ttcgaccgct tccctgaaag tcgaagcgac caatttgatg
     1741 accggcgctt cctcggccac tcctttggct tcttcgagcg ccaccggccg ctgcaacgaa
     1801 gacaagccgt cgccggattt gattcccaaa gattttatcg ccgcttcgac ttcggatttg
     1861 taaggattgt atctccgaag caccaactcc agatccgccg gggtgatgat gaaaactccc
     1921 aaattcaaac gattggcttt ggcgatgaat ctcaaagcgt cctgggcccg gctgttttgc
     1981 ggatcgacca tgccgacgac gagcatgtct ttggtccgcg aaatcggagc caccttatag
     2041 gcttggaccg tttccttcgg caccagggcc aaaagatcgt cggtcaaaga gcccacgttt
     2101 attttttgat aaggaatgcc gatgatctcg cttttgactt cggcgatctt ttcttcgggc
     2161 acggttcgcc gcagataaag cagatcttcg accggctttt gggaaaccgc cgattctttc
     2221 aaaatctgct cgccggcttc tttggtgagc agatttttct taacaagggc tgccgttaaa
     2281 acctgggaat ccattttaga aagcggcgaa gggattttct cttccgaggc tgttcggtcc
     2341 gattaccggg agattgatcg accttcccgg atcttccgaa gagaagaatt cgtcttggag
     2401 cacttcctgc ggattgaatt gcaagccgct caaatcggcc acggactgga ggttggccgg
     2461 caggatcgcc tcgatcagcg gcgccggagt gaagaaaaga taataggcgc cgatcgcgag
     2521 aatagcgagc gccgccgcga cggaaatgat gatgaacgtt ctcgaccccc tttgattcgg
     2581 ttgaatgatg accgccattt tattggtaat atgtgttgat gattaattgg agggaataaa
     2641 tattggctct tcccgaggcg ctgatattca tcgaaacaat gtccatcagc ctcatgttgt
     2701 tctcgacgtc gccgataaaa gctttaagac cgtcgtaaga gccttcggcc ctgaccgtgg
     2761 ctcgcaaaac cccgtaagcg gaaacgagag ggcttccgtt cgactgcatc ggcggatgct
     2821 ggaaattgac gctcgccacc accatttgat tcgtggacgc gagaccgtaa atctgcgcca
     2881 aaacatgggg aatgtcgtca ttcaaaggca aggagcggtc gagatcgtct ttgtattgga
     2941 agctgtcttc gtattgctgc gccaaattgg cgaaagcttc cacctgggcc tccgccatcc
     3001 gcagaacaac ggccgcggcg tccctgtccg cccgaagctg cttcacgccg ttgtcgcctt
     3061 cgaattccgg cctgatgaaa ttggtgaaca cgaaaacggc cgcgaccaaa agggcgatgc
     3121 ctcccaaaat actcaaaaat cttttaaagg aaaaagccat tgttgaaaat tatttgcatt
     3181 tggaaagata ctccggtctg gccgagagtc gctccgccta tggaaacgct ttccacttcc
     3241 tgaagattct ggaaaacgtc gagttggctt gctaaagatt cgtaggttga ggctgttcca
     3301 ccgagattcg ccgtcaggct cggaatatcg aggtccatcg agctgtaaga aatgtcttgt
     3361 ctagtattcg tctcgagaaa cgaaaatatg ttggaagaga aaacatgtct cgaaattaat
     3421 gttttcaggt tttctatctg ggaatagaaa tctatgaacg tctgcctgtc ttgcggcgat
     3481 acttgttcgt taagcgcgtc gatctgcgcc tggatactct ccgtgtcgct caagagaaaa
     3541 ggcttatacc cgtaagccaa tccgaaatag cttactccca agagaatgag aataaacaga
     3601 gaaaatatca aaagtcttgt cggccaaccg gcgggctccc ggccgacatt gaatctttga
     3661 tcgatatttg tcgggtattg cggtggcatt ttttaaaatt gccttaatcc gaggccgatc
     3721 gcgaccgcga gcgttccgcc caattcgttc accacgggct ggagcgtcgg cgcgtagctc
     3781 aaagcggaaa atgggctcgg cctttcgact ttcatttgca ggaaatccgc ggcgtattta
     3841 tccaagccgg caagctcgcc gcctccgccg gaaagaatca cctttccgat ttggccgcga
     3901 tagttcttct caaaattgtc tttcactcgt cttacttcat taagtataac atcgacgtaa
     3961 ggcagcatta aagtggacaa ctcgtattct ccgcccattc cgagcaagcc tcggcgtttt
     4021 ttcagttctt cggctctttt gacgttgata ttcaagccgt tcgcgatcgc ctggctcatg
     4081 gaaccgccgg cgtaatcgag ataggcgctc gacagaagat ggccgtctct cgcgacatag
     4141 agactggtcg atcttccgcc gatatcgagg atcaaagaaa gctccgcgtc ccctttcgtg
     4201 aaaaccctgg ccaggctcat tccttcgatc tcgatcaccc tcaaattaag accggcggcc
     4261 tggaaaacgc ttttgtattt ctcgacgtct tcgaattctc cgaccaccga ccattcggtc
     4321 gccaccgagg cgatggccgc cggcaccaag gctcgcgcct ggtaagacat gatctgatcc
     4381 atttcgtttt taggcatcat cggcagttcg agtaaagtgg tgaaagcgga aaaaggcggc
     4441 agagaagcga aaaccgcggc cgctccgggc ttcacttccc tgacgagggt ctggagcatg
     4501 ctcgccgctt ctttgtcgtc cattttcaaa ctgctcgttt ggatggcgct gttctgccgg
     4561 gaaagatggc tgtaagtttc cagtagtccg tagtttttga aatgcggccg atcggcgaca
     4621 atggccagtt cgacgagttt aatcgaagtt gttccgatgt cgaccccgat ggccgactgg
     4681 aaagaaagca aaggttttag gggtttcaaa aaatcgagta tcgccatgaa ttgctattat
     4741 taatgatagc acgaatgccg agcttcacga atatcaaaaa tcagcttttg gatcttgttt
     4801 ttccgaagct ttgcttgaat tgccgttgcc ttctagaaaa accgatcgac aaagaaaatc
     4861 ttttgtgcgg ccgctgtttc ggttcgatcg tcgtcaatcg tctgatattc ccggaaccgg
     4921 gatttttcct cggtagcgcc accctttaca ggatagacgc cgcgaggaat ctggtgcatt
     4981 tcctgaaata cgaagatttt cccggggtaa aacacccgat cgcaaaaata atcgaagcct
     5041 atctccgaaa atccgctttc atccatcttt ttcgcaatgt aaagccggaa atcgttccta
     5101 ttccccttca tttcatgaaa tccataagcc gtgggttcaa ccaagccgaa gacatcgccg
     5161 aagttttggc gagcggaacc ggctggccaa ttaatactcg cctgctccta agaaccaaaa
     5221 atacacagcc ccaagccaaa ctcgaacaaa aatcggaaag aatggaaaac atggctggcg
     5281 cttttcgcat aaaaaacggc ttggaagaat acgtttccgg gaaaagcttc gttgttgtcg
     5341 acgacgtcta tacttccggc gccaccatgt ccgaagcgat caagatattg aaatccggcg
     5401 gcgccaaaag cgtcgccgga ttcgtattcg cgaaaggtta aaactaacag ggtccgtcta
     5461 cgcagctcca agatccgctc caaccgccgt gagacgaaca tctgctaagg catgtttcgg
     5521 ggcctccaac gtattggcat tttactccgg cgcccaaact tcctgacggc gtataaccgt
     5581 cgttgcaggt gcagctccaa cttcccggac tgcatgattc gcatataaag atccagttgc
     5641 agaactttcc ggatgaacaa tcgga
//
LOCUS       DAOPQT010000022         5651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_15665_length_5651_cov_4.052180, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000022 DAOPQT010000000
VERSION     DAOPQT010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15665_length_5651_cov_4.052180"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /gene="mraW"
                     /locus_tag="PKG74_00905"
     CDS             <1..228
                     /gene="mraW"
                     /locus_tag="PKG74_00905"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HOB89875.1"
                     /translation="KDILEAIPEIIVSKGRVAVITFQSLEDKTVKEIFKKQAKEGKAE
                     LLYPKPIDPSLQEIRSNPRSRSAKLRAIRVI"
     gene            236..529
                     /locus_tag="PKG74_00910"
     CDS             236..529
                     /locus_tag="PKG74_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89876.1"
                     /translation="MTIIQPNQNKNPFRKLNIFLIVALFVCVGVSVGLCSMAEGFRYE
                     AKKLEKEIDGAQLVNAELKGKIMTAVDAENLDLMALEKGLIEDKNPQWVFALY"
     gene            508..2124
                     /locus_tag="PKG74_00915"
     CDS             508..2124
                     /locus_tag="PKG74_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOB89877.1"
                     /translation="MGVRFILIVLVFSAAYMGLGFHFYNLQIENGQQYLAKAENLRAG
                     ADLVEPERGSIFFTDKNGNSMPAAIKKEYQNITAVPKKITDPETTSEILAPILGMKKS
                     DLLVKLDRPESSYAMLMKKVPEAIAKEIKSLNLEGIQVEKEETRFYPYGTLAAHVLGF
                     VREGVGQYGVESFYDEDLVGSVNDSISGKWIGAENGEDIKLTIEPNIESQAEKTLQNL
                     VATYKAEGGLVIVGDPKTGEILAMAGTDNFDPNNYGSASMGSYLNKSVQEVYEPGSIF
                     KIVTMAAGIDSGKITPQTTYYDNGSVKIDGHTIENWDHKAHGTMTMAGVLEKSLNTGS
                     IFAVEQMGKKTFWNYIEKFGFNEKTGIDLPGERLSDTKNLKNGRDINYATASFGSGVA
                     VSPIRLWTSIASIANKGVMMKPHVVKADPVMIGRSVSEETAKKVTDMLVSAVDNGKLA
                     TISGYTVAGKTGTAFIPDLVNGGYTEDVINTYIGFAPASDPKFVILLRMDKAEGAPLA
                     GQTIIPAFRKLAEFILRYYEVAPDRPNPMN"
     gene            2166..3485
                     /gene="murF"
                     /locus_tag="PKG74_00920"
     CDS             2166..3485
                     /gene="murF"
                     /locus_tag="PKG74_00920"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOB89878.1"
                     /translation="MKKTFLKLLEIVLKGLAKATLEKYDPQIIGITGSVGKSSTKEAV
                     LSVLKTKKSVRANRGNLNNELGLPLTILGDYDYYREDSKRKTGFWFKVIFEAVAGLLK
                     RRKDYPEVLVLEYGANKPGDIEYLLEIAKPDISIITAISETPVHVEFYPNPEALAREK
                     SRLVSALSAKGTAILNFDDNIVMDMKEKTRGRIITFGFKEGADIRAVNFENQSDGLRP
                     IGLSFKMEHKGSAVPVRMSNIFGRSYVYNMLAAAAVGLKNNLNLVEIASALMLYRPLP
                     GRMRLIKGEKNTWILDDTYNAAPASTEEALEALKSLPAKRKVAVLGDMAQLGEYSVQS
                     HEAVGKKAAGIADIIVTVGAKARFIAEAGRKKGFPKDNIYTFDNSDNAKTIVEEIIKE
                     GDLILIKGSQVTRMEKIVMEIMAEPEKAKDLLVRQYGRWLGEYYIDM"
     gene            3521..4765
                     /locus_tag="PKG74_00925"
     CDS             3521..4765
                     /locus_tag="PKG74_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOB89879.1"
                     /translation="MFHFSFRSCPSFTLIEVLVVIGILGVLTGITVVVLNPSELLASA
                     RDTTRLSDLETVNKALFLYTTQKGEPSGNPNLLYLSLPDTNSHCASYILPTLPSPYEY
                     SCVTEANLRKVDGFGWIPVNLSSIPGGSPLAVLPVDPTNNSSFYYTYITGGSYELLSL
                     LESENHLKTSSLNDKGYDPVRFEIGTNLSLWNNPSGLVGYWPLEGSGTVTNGQTLGLE
                     DRSGRNNHGTASNANGSGMSFVQGKVGSGIQLDAVDDGVDCGNSPSVNLTTELTITAW
                     VKFTGLKWVNSIVEKGKENTNNLYWLFRGDDATRHQWTFEFGNGINRSTLDVNETINV
                     DQWYFVAATFNNGNIALYRNDALVGSKSTAITYLDTTGYNAWIGRYSPGGHLLNGAAD
                     EVRLYSRALSVDEIGSLYKSGK"
     gene            4869..>5651
                     /locus_tag="PKG74_00930"
     CDS             4869..>5651
                     /locus_tag="PKG74_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOB89880.1"
                     /translation="MPLFEPSFFSGRKSFTLIELLVVIGILAALTAATVVVLNPSELL
                     AESRDSVRLSDLRSLNEMLFIYTTQRGEPGGNQNYVYLSLPDNNVNCSSYSLPGLPAP
                     YQYSCVTEANLRKINGSGWIPVDFSSIPGGSPIPTLPVDPANSVSYYYTYMSGGSYEI
                     TALLESERYLKSSALKDKGIDPARMEMGTNLSLWNLPSGLVAYWPFDGSGSIVNGQTL
                     GLEDQSAFQNNGTASNANGSGMAFVEGKVGNALYLDGIDDWVN"
BASE COUNT         1495 a         1416 c         1411 g         1329 t
ORIGIN      
        1 aaagacattc tcgaggccat tccggaaata atcgtctcca agggcagggt ggcggtgatc
       61 accttccagt ctttggaaga caaaacagtc aaagagattt ttaaaaaaca ggcgaaagaa
      121 ggcaaagcag aacttttata tccgaaaccg atcgatcctt ctttgcaaga gataagatcg
      181 aaccctcgga gtcgatcggc caaactccgg gcgatccgcg taatctaacc gtaaaatgac
      241 cattatccaa ccgaaccaaa acaaaaatcc gtttagaaag ttgaatatct ttctgatcgt
      301 cgcgcttttc gtttgcgtcg gagtgagtgt cggtctttgt tccatggccg aaggcttcag
      361 atacgaagcc aaaaagctgg aaaaggagat cgacggggct cagctcgtga acgccgagct
      421 caaaggaaag atcatgaccg ccgtcgatgc ggaaaacctc gatctcatgg ctttagagaa
      481 agggctgatt gaagacaaaa atccgcaatg ggtgttcgct ttatattaat cgttctggtt
      541 ttttccgcgg cctacatggg ccttggattc catttttaca atctgcagat agaaaacggc
      601 cagcagtatt tggccaaagc cgagaatttg cgggccggag ccgatcttgt cgaaccggaa
      661 cgggggagta ttttttttac cgacaagaac ggcaattcga tgccggccgc catcaaaaaa
      721 gaataccaga atatcacggc ggtcccgaaa aaaatcactg atccggaaac aacgtcggag
      781 atcctcgcgc ccatcctcgg aatgaaaaaa tccgatcttt tggtaaagct cgatcgaccc
      841 gagagttcat acgcgatgct catgaaaaaa gttccggagg caatcgccaa agaaatcaaa
      901 agtcttaatc tcgaaggaat ccaggtcgag aaagaggaaa ccaggtttta tccctatgga
      961 acactcgcgg cgcatgtttt ggggtttgtc cgcgaaggcg tcggccagta cggcgtggaa
     1021 tccttctacg acgaggattt ggtcggttcg gtcaacgatt ccatttccgg taaatggatc
     1081 ggagcggaaa acggggaaga cattaaactt accatcgagc cgaatatcga atcgcaggcg
     1141 gagaaaactc ttcagaatct cgtcgctacc tacaaagccg aaggagggct tgttattgtc
     1201 ggcgacccca aaaccggaga gatcttggct atggccggaa cggacaattt cgatcccaac
     1261 aattatggtt ccgcttccat gggaagttat ctcaataaat cagtgcagga agtctacgaa
     1321 cccggatcga ttttcaagat cgtcaccatg gctgccggaa tcgactccgg aaaaatcacg
     1381 ccgcagacga cttactacga caacggttcg gttaaaatcg acggccacac catcgagaac
     1441 tgggaccaca aggctcacgg caccatgacc atggccggag tcttggaaaa atccttgaac
     1501 accggaagca tcttcgccgt agagcaaatg ggtaaaaaga ctttttggaa ctacatcgag
     1561 aaattcggat tcaacgagaa aaccggcatc gatttgccgg gagagcgctt gagcgacacg
     1621 aagaatttga aaaacggccg ggacataaac tacgccacgg cttctttcgg gagcggggtg
     1681 gccgtgtctc cgatcaggct ttggacttcg atcgcctcca tcgccaacaa aggcgtgatg
     1741 atgaagccgc acgtcgtcaa agccgatccg gtcatgatcg gccggtcggt ttccgaagaa
     1801 accgccaaaa aagtgacgga catgctcgtt tccgccgtcg ataacggtaa gctggcgacg
     1861 atcagcggct ataccgtggc cggaaaaacc ggaaccgctt ttatcccgga tttggtgaac
     1921 ggaggctata ccgaagacgt catcaacacc tatatcggat ttgctccggc ttcggacccg
     1981 aaattcgtga tccttttgag gatggataag gccgagggcg cgcctttggc cggacaaacc
     2041 attattccgg ctttccgtaa actcgcggaa ttcatccttc gctattacga agtggcgccg
     2101 gatagaccga atcccatgaa ttagatacaa tggcttgtcc cggtgaagtc cgggattgaa
     2161 aaccgatgaa aaaaacattt ttgaaattgc tggagatcgt tctgaaaggg ctcgccaagg
     2221 cgacgctcga gaaatacgat ccgcagatta tcggcatcac cggcagcgtc ggcaagagct
     2281 cgaccaagga agcggtgctt tccgttctga aaaccaagaa aagcgtccgg gcgaaccgcg
     2341 gtaatttgaa caacgaactg gggcttccac tcaccatttt gggggattac gactattatc
     2401 gcgaagacag caaaaggaaa accggatttt ggttcaaggt gattttcgag gcagtcgcgg
     2461 gcttgctcaa gcggcgaaag gattatccgg aagttttggt tttggaatac ggagcgaaca
     2521 aacccggaga catcgaatac cttttggaaa tcgcgaagcc ggatatctcg atcatcacgg
     2581 ccatttccga aaccccggtc cacgtcgagt tctatccgaa tcccgaggct ttggcgcgag
     2641 agaaatcccg cttggtttcc gctttgtccg ccaaaggcac ggccattctg aatttcgacg
     2701 acaacattgt catggatatg aaagaaaaaa ccagaggtcg gataatcacc ttcggattca
     2761 aggaaggagc ggatatccgc gccgtcaatt tcgagaacca gtcggatggt ctgcggccga
     2821 tcggtttgtc tttcaaaatg gaacacaagg gttcggccgt gccggtcagg atgtcgaata
     2881 ttttcggacg gagctatgtc tataacatgc tcgcggccgc ggccgtcgga ctgaaaaaca
     2941 acctgaattt ggtcgaaatc gcttcggctt tgatgcttta ccgtccgctt cccggaagga
     3001 tgaggcttat caagggcgag aaaaacacct ggattttgga cgatacctac aacgccgctc
     3061 cggcttcgac cgaagaagct ctggaggctc taaaaagcct tccggccaag cgcaaggtgg
     3121 cggttttggg agatatggcc caactcggag aatattcggt ccagagccac gaggctgtcg
     3181 gcaaaaaagc ggctggaata gccgacatca tcgtaaccgt cggcgccaaa gcgaggttta
     3241 tcgccgaagc cggacggaaa aaaggattcc cgaaagacaa catttacacc tttgacaatt
     3301 ccgacaatgc gaagacaatc gtcgaagaga tcatcaaaga aggggatttg atactcatca
     3361 aaggttcgca ggtgacgagg atggaaaaaa tagtcatgga aataatggcc gaaccggaaa
     3421 aagccaagga tctgcttgtg cggcagtacg gacgctggtt gggggaatac tatatcgata
     3481 tgtaatcggt tcgaggcttt gaggtataat taagttcttg atgtttcatt tttctttccg
     3541 ttcctgtccc tccttcaccc tcatcgaagt cctcgtcgtc atcggaatcc ttggagtttt
     3601 gaccggtatc accgtcgttg tcttgaatcc gtcggaactc ttggcttcgg caagagacac
     3661 gaccaggctt tccgacctcg agaccgtcaa taaggctctt tttctctata ccacccagaa
     3721 aggcgagcca agcggcaacc cgaacttgct ctatctttct ttgccggaca cgaattccca
     3781 ttgtgcctct tatattcttc cgactcttcc ttctccctac gaatattcct gcgtcaccga
     3841 agcgaatttg agaaaagtag atggttttgg ctggataccg gttaatttat cttccattcc
     3901 cggaggttct cctctggccg ttcttccggt agatccgaca aacaattcct ctttttacta
     3961 tacttatatt accggaggtt cttacgaact tctttccctt ctcgaatccg aaaaccacct
     4021 caaaacctct tctttgaacg acaaaggtta cgatcccgta agattcgaga tcggaacaaa
     4081 tctctccctt tggaataatc cttccgggct tgtcggttat tggccgcttg aaggatccgg
     4141 gaccgtcacc aacggtcaaa ctctcggcct cgaagacaga tccggtcgca acaaccacgg
     4201 aaccgcttcg aacgccaatg gttcggggat gtcgtttgtc caaggaaaag tcggtagcgg
     4261 catacagctt gacgccgtag atgacggagt cgactgcgga aacagtccga gcgtcaattt
     4321 gacaaccgaa ctcaccatta ccgcctgggt gaaattcacc ggtctgaaat gggtcaattc
     4381 gattgtggag aaggggaaag aaaataccaa caacctttat tggttgttta gaggtgacga
     4441 cgcgacgcgt catcaatgga cttttgaatt cggaaacgga atcaatagat ccacgttgga
     4501 tgtcaatgaa accattaatg tcgatcaatg gtattttgta gccgccactt ttaataacgg
     4561 caatattgct ttatacagga acgacgcgct tgtcggttcc aagtcgacgg ccattacgta
     4621 tctcgatacg accgggtaca acgcctggat cggaaggtat tctcccggcg gtcatctttt
     4681 gaacggcgcg gccgacgaag tgcggttgta cagtcgcgct ttgtcggttg atgaaatagg
     4741 atcattgtat aaatcgggaa aataatccaa aaatatgcca aatatcatag ataacccgat
     4801 cggtttattc aaagtcacac agaatatctt tttattaata aaaagttgga cctgttataa
     4861 tttacaatat gccgcttttt gaaccctcgt ttttctccgg ccggaagtcc ttcaccctta
     4921 tcgaacttct cgtcgtcatc ggcatcctcg ccgccttgac cgcggccacc gtcgttgtcc
     4981 tgaatccctc ggaacttctc gcggaatctc gggactcggt gaggctttcc gacctcagat
     5041 ccttgaacga gatgctcttc atctatacca cccagagagg cgaacccgga ggcaatcaga
     5101 attacgtcta tctttccctt ccggacaata acgtcaattg ttcttcgtat tctttgccgg
     5161 gtcttccggc tccttaccag tattcctgcg tcaccgaagc gaacttgaga aagattaacg
     5221 gttccggctg gattccggtc gacttctctt ccattcccgg aggttctccc atacccactt
     5281 taccggtcga tccggcaaac tccgtttcct attactacac ttacatgtcc ggcggatcgt
     5341 acgagatcac ggccttgctc gaatccgaga gatatctcaa atcttcggcc ctgaaagaca
     5401 aaggaatcga tcccgcgaga atggaaatgg gaaccaacct ttccctgtgg aatcttccgt
     5461 ccgggctcgt cgcttattgg cccttcgacg gctccggatc gatcgtgaac ggccagaccc
     5521 tcgggctcga agaccaatct gctttccaga acaacggcac ggcttcgaac gcgaacggtt
     5581 cgggcatggc ctttgtcgag ggaaaggtgg ggaatgctct ttatcttgat ggaattgacg
     5641 attgggtgaa c
//
LOCUS       DAOPQT010000023         5621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_15793_length_5621_cov_3.145706, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000023 DAOPQT010000000
VERSION     DAOPQT010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15793_length_5621_cov_3.145706"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    593..602
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            775..1776
                     /gene="trpS"
                     /locus_tag="PKG74_00935"
     CDS             775..1776
                     /gene="trpS"
                     /locus_tag="PKG74_00935"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOB89881.1"
                     /translation="MAKKIILTGDRPTGKLHLGHYIGSLTNRLKLQDETEEYVMIADA
                     QALTDNADNPEKVRQNVLELMLDYLAIGLDPEKTTFLVQSMIPELPELTVYFLNLVTL
                     ARLQRNPTVKNEMKQKGYGANVPAGFLTYPISQAADILAFKADLVPVGEDQLPMIEQT
                     NEIADTFNRFYGPVFKHAKALVSSTGRLPGIDGKAKMSKSLGNAIYFSDSEKTIRDQV
                     MEMYTDPKHIHVKDKGKVEGNVVFTYLDAFDSDKSVVEALKKQYRKGGLGDVEIKKRL
                     IDVLESLISPIRQKRERLAKDPKAVMGILEKGTKKAREATKKTLAEAKAAMKIDYFR"
     gene            2031..3167
                     /gene="tgt"
                     /locus_tag="PKG74_00940"
     CDS             2031..3167
                     /gene="tgt"
                     /locus_tag="PKG74_00940"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOB89882.1"
                     /translation="MIDFRVLKESPKSRARLGILKTPHGEIETPALVGVATQATVKTL
                     TSEEAREAKLRLLICNTYHLHLRPGEKIVKANGGLHKFMNWHSGLMTDSGGFQVFSLG
                     FGRDFGLSKVSKSGLKETLEEGSQPKSLKITDDGVFFKSHLDGREMFLGPKESIKIQE
                     DLGADIILAFDECPPPIANREYVLKSLERTHRWAKECLRVKKSKQALYGIVQGSRFKD
                     LRKESARFIDSLGFDGYAIGGEFGSDKSSMVKMLETVNAELDSSKPRHLLGIGYLEDI
                     PKIVKSGVDTFDCTAPTHYGRRGITFVSSGKLDLNKAIFLKDRKPIDRNCGCPVCGNY
                     SRSYLSHLIRAKEISGLKLLTVHNLFFFNTLIENIRKEIKKGKF"
     gene            3190..4224
                     /gene="queA"
                     /locus_tag="PKG74_00945"
     CDS             3190..4224
                     /gene="queA"
                     /locus_tag="PKG74_00945"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOB89883.1"
                     /translation="MQFEKLLELYDYRFPEKAIAQCPASPRDSAKLLIYDRKTEKVFL
                     DVFRNLGDHLPPKAVLVLNETKVIPARIRAKKSTGGKVELLYVGEEKGNLRFMVNRKI
                     RPGEKIFLDSRNFLTLVDQKEGIGLFEPSFPARNIFGVLEKFGETPIPPYIKHSPLKE
                     PELKEKYQAVFARVRGSVAAPTASLHFTKRLMAKLQKSGFDLKFVILHVNLGTFAKLT
                     PEQIKRSKLHEEFYEIDPGMAAFLNKAKNQGRPIVAVGTTVVRTLESASDAKGNLTKL
                     SGETSLFIKEGYRFRFVDSLITNFHVPKSSLLMLVSAFAGRKQILDLYRKAIAKDFRI
                     FSFGDGMLLK"
     gene            4287..4619
                     /locus_tag="PKG74_00950"
     CDS             4287..4619
                     /locus_tag="PKG74_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89884.1"
                     /translation="MRLFFALMLFTGTLGGVAEKCVELKNREFLKTIAAMAIVFPLVI
                     ALVKIFPEVAADAAIPDTWPGLIASLAVNLLGNFWASRVCPEWHFAPIGLVKELSRRV
                     KSRISRKR"
     gene            4704..5411
                     /locus_tag="PKG74_00955"
     CDS             4704..5411
                     /locus_tag="PKG74_00955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOB89885.1"
                     /translation="MKQHRFIGDFAPEGNYLEIRDEELVNQFKNVLKLKPGEEIILAD
                     GQGSEFTGAIKSFHPKFVALTVGEKRRNSNDPATAVILYCSLLKKENFEWVAEKATET
                     GARAIVPVISDRTVKLNLNIGRLRKIVKEAAEQSGRGFLPEVSEIIDFDEAVEQAKDN
                     ELNIFFDPKGKVFEKPQWFSMYGDKRIGVFIGPEGGWTDREIAAAEAAGFQIVSLGKL
                     VLRAETAAIVAVYLAAH"
BASE COUNT         1530 a         1315 c         1433 g         1333 t
ORIGIN      
        1 gagccgtacg acgcaaagaa gcatcaggat gcgcgggttg gctttactcc tcttacgatt
       61 tggccgaggc atggcgtacc agcccgttta cttcgtagag acaacaatgg cttggttgga
      121 ggtaccctct atctttgccc tgattgtgtc gccaagggag atctacgaaa acaggaagat
      181 aagtttatgg aacgagtaag atctcttctg atgatagaga tctgtgaact ggcctaggcc
      241 ggtgttctgg cgcctacggg cgctcttgat tccttggggc tttccgaaga ggaaaagcaa
      301 tgtcggatca atagcatcca gacggtcatc gagcatttcg aggccatcga tccagaatac
      361 actccttatc atcctaggtt tgtgtccatt ttacagaaat gccggaagct tctgggaggc
      421 acctcctatc acgcaaaagg tccacgaact aaggagaagg agtttcagga tcgagcgaga
      481 tctcttctta tagaagaagt ctgcgagttg acctgggctg gtgtcctcac gccaacaggc
      541 atcccgctct atttgaggct tttcaaaaaa aagagacagc ctcggataga gannnnnnnn
      601 nntatcgaca atgtcgaagc catcaacccg aaagatgttc cttatcaccc gaaagccata
      661 tccgttttaa aggagtgttt agcactcctg aaacgtgctt tctaacggtt aggtccggcg
      721 gccgagtaat ctcggccgtt tttcttttta gatggcaaaa actataatgt acttatggct
      781 aagaaaatca ttttgactgg agacagacca accggaaagc tgcatttggg gcactatatc
      841 ggttctttaa cgaaccgttt gaaacttcag gacgaaaccg aagaatacgt gatgatcgcc
      901 gatgcccagg ccttgaccga taacgccgac aatccggaaa aagtcaggca aaatgttttg
      961 gaattgatgc tcgattatct cgcgatcggt ctcgatcccg agaaaaccac ttttctggtc
     1021 caatcgatga ttccggaact tccggaattg actgtttatt ttctgaatct ggtcaccttg
     1081 gcgagactgc aaagaaatcc gacggtcaaa aacgaaatga agcaaaaagg atacggcgcc
     1141 aatgtcccgg ccggtttttt gacttatcct ataagccagg cggccgatat tttggctttc
     1201 aaggccgact tggttccggt cggagaagac cagctgccga tgatcgaaca gacgaacgag
     1261 atcgccgaca ctttcaacag attttacgga ccggtattca aacacgccaa agctttggtt
     1321 tcttcgaccg gccggctgcc gggaatcgac ggcaaagcga aaatgtcgaa atccttggga
     1381 aacgccattt acttttccga ttcggaaaaa accatcagag atcaggtgat ggagatgtac
     1441 accgatccga agcacatcca cgtcaaagac aagggaaaag tcgaaggcaa tgtcgttttt
     1501 acctatctcg acgcttttga ttcggacaag tccgttgtcg aagctttaaa aaagcaatac
     1561 cggaaaggcg ggttgggaga cgtggaaatc aaaaaacgtc tgatcgatgt tttggaaagc
     1621 ttgataagcc cgatcagaca aaaaagggaa cgcctggcta aggatccgaa agcggtgatg
     1681 ggcatcttgg aaaagggcac taaaaaagcc agagaggcaa ccaaaaaaac cctggccgaa
     1741 gccaaggcgg cgatgaaaat agactatttc cgataaaccg gccgtttgtt ggccggcgtg
     1801 ctataatgcg ggaaaggtcg tcaacgtcaa tttcgacatg tgccagactt gcggatgttc
     1861 tccctgcaaa aagtgcggca gggaaataaa aaacggcgtc tgcagcggtt gcggcaagag
     1921 atctgacgag tgcgaatgca agagggaagg ataagcgggc gtttcaatcc cgcggtcaag
     1981 ccgcgggatt tttgtttccc gaagattttc gtgttatgac ttatggcgaa atgattgatt
     2041 tccgagtttt gaaagaatct ccgaaaagcc gggccaggct cggtattttg aagacgccgc
     2101 acggcgaaat cgaaaccccg gctttggtcg gagtagccac ccaggcgacg gtaaaaacct
     2161 tgacttccga agaagcgcga gaggcgaaat taaggctcct gatttgtaac acttatcatc
     2221 ttcatctccg gccgggagag aaaatcgtca aagctaacgg aggacttcat aagttcatga
     2281 attggcattc ggggctgatg accgactccg gcggtttcca ggtattcagt ctgggattcg
     2341 gccgggattt cggattgagc aaggtctcca aatccggttt aaaagaaacg ctcgaagaag
     2401 gcagtcaacc gaaaagcctg aaaatcaccg acgacggcgt gtttttcaaa tcccatctcg
     2461 acggacgaga aatgtttttg gggccgaaag aatcgatcaa aatccaagaa gacctgggag
     2521 ccgacattat tctcgctttc gacgaatgcc ctccacccat cgccaaccgc gaatacgtct
     2581 taaaatccct ggaacgcacc catcgctggg ccaaggagtg cctgcgcgtc aaaaaatcca
     2641 aacaagccct ttacgggatc gttcagggaa gtagattcaa agatctgcgg aaggaatcgg
     2701 cgcgattcat cgattcttta gggttcgacg gctacgccat tggcggagaa ttcggaagcg
     2761 acaaatcgtc catggtgaaa atgctcgaaa cggtgaacgc cgaactcgat tcgtcgaaac
     2821 cgagacatct tttgggaatc ggctatctcg aagacattcc gaaaatcgtc aaatccggcg
     2881 tggatacttt cgactgcacc gcgccgacgc attacggccg tcgaggcatc acctttgtct
     2941 cttcgggaaa actggatctg aacaaggcga tatttctgaa agaccgaaaa ccaatcgacc
     3001 gaaattgcgg ctgcccggtc tgcggaaatt attcccgaag ttatctcagt catttgatcc
     3061 gcgccaaaga aatttccggc ttgaagcttc ttaccgtcca caatcttttt ttcttcaaca
     3121 ctttgataga aaacatcagg aaagaaatca aaaaagggaa gttttgattt ctcggatttg
     3181 gaaaaaataa tgcaattcga aaaacttctg gaattatacg attaccgctt tcccgaaaaa
     3241 gctattgccc aatgtccggc gagccccagg gacagcgcca agcttttgat ctacgaccgg
     3301 aaaacagaaa aagtttttct cgacgttttc cggaatctcg gcgatcatct tcctccgaaa
     3361 gcggttttgg ttctcaacga aaccaaggtg attccggcgc ggatccgggc caagaaatcg
     3421 accggaggga aagtggaatt gctttatgtc ggagaagaaa aaggaaatct tcgattcatg
     3481 gttaaccgca aaatccgtcc gggagaaaaa atattcctgg atagccggaa ctttttgact
     3541 cttgtcgatc aaaaagaggg gatcggtctt ttcgagccgt cttttccggc gcgaaacatt
     3601 ttcggcgttt tggagaaatt cggagaaacg ccgattcctc cgtatatcaa acattcgcct
     3661 ttgaaagagc ccgaactcaa agaaaaatac caggcggttt tcgcgagagt ccggggttcg
     3721 gttgccgcgc ccaccgcctc gctccatttt acgaagaggt tgatggcaaa gcttcaaaaa
     3781 tccggatttg atctgaagtt cgtcattctc cacgtcaatc tggggacttt cgccaagctg
     3841 acgccggaac aaataaagcg ttccaagctc cacgaagaat tctatgaaat agatccggga
     3901 atggcggcat tcctgaataa agccaaaaat caaggccggc cgatcgtcgc cgtcggcacg
     3961 accgttgtcc ggaccctcga atcggcgagc gacgccaagg gaaatcttac gaaactgtcc
     4021 ggggaaacga gtcttttcat caaagaagga tatcgattca gattcgtcga tagcctgatc
     4081 accaattttc acgttccgaa gtcgagcctt ttgatgcttg tttccgcttt cgccggcaga
     4141 aagcaaatcc tcgatctcta tcggaaagcg atcgccaaag acttcaggat attttctttc
     4201 ggagacggga tgctcttgaa ataacttgat tcccgttgat tttgatgata atgattcgtt
     4261 agttcttgat aaaggaggag taatatttgc ggttattctt tgccttgatg ctctttacgg
     4321 gaacgctcgg gggcgtcgcg gaaaaatgcg tggagctcaa aaatagagag ttcctgaaaa
     4381 caatcgccgc gatggccatt gttttcccgc ttgtgattgc gctagtcaag attttcccgg
     4441 aggtggcggc cgatgcggcc atacccgata cttggccggg attgatcgcc tcccttgccg
     4501 tcaatctctt gggcaacttt tgggcaagcc gtgtttgtcc cgaatggcat tttgctccaa
     4561 ttggtttggt gaaggagttg tcgagacgcg tcaaatcgag aatatcccgc aagcgttgat
     4621 acggacggag tcggaactcc gtccttttta ttatgattgc cgtctacatg aagacgcgtt
     4681 aagcttaaaa tatcctcgct tatatgaaac agcatcgatt catcggcgat ttcgcgccgg
     4741 aaggaaatta tctggaaatc cgggacgaag aactcgtaaa ccagtttaaa aacgtcctca
     4801 aactaaagcc cggagaagag ataatcttgg ctgacggcca aggttcggaa tttacgggag
     4861 caatcaaatc tttccatccg aagttcgtcg ctttgacggt cggagaaaaa agacgaaatt
     4921 cgaacgaccc ggcgacggcc gttattttgt attgctcttt gctcaaaaaa gagaatttcg
     4981 aatgggtggc ggaaaaagcg accgaaaccg gcgcgcgggc aatcgttccg gtgatttccg
     5041 accggacggt caaattgaac ctgaacattg gccgtttgcg gaaaatagtc aaagaagcgg
     5101 ccgaacagtc gggccgcgga ttcttgcccg aagtttcgga aataatcgat ttcgacgaag
     5161 cggtggaaca ggccaaagat aacgagctca atattttttt cgatcccaag ggaaaggtat
     5221 tcgaaaaacc ccagtggttt tcgatgtacg gagacaaacg gataggcgtt ttcatcggtc
     5281 cggaaggagg ctggaccgac agggagatcg cggccgccga agccgctggt ttccaaatcg
     5341 tttctttggg caaacttgtc ctgcgggcgg aaacagcggc catcgtcgcc gtttatcttg
     5401 cggctcactg attttataaa aagagaagag cggtcaaagc cgctcttgaa tcaaccatcg
     5461 tctcccgcca ccaaaacgtc tatccggaaa gatttttggt agcacgatgg atcgatggtg
     5521 tagccgtttt cttcgagcca agaaaggacg cgctcctgct cttcgggaga attgccccag
     5581 atatagccgg ggtttttctt gtgcgctttt ctttcgcatt c
//
LOCUS       DAOPQT010000024         5551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_16036_length_5551_cov_3.208697, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000024 DAOPQT010000000
VERSION     DAOPQT010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16036_length_5551_cov_3.208697"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PKG74_00960"
     CDS             complement(<1..570)
                     /locus_tag="PKG74_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOB89886.1"
                     /translation="MKNYPFRKIEKKWTAFWLKKKVFQPDLKKAKKPFYNLMMFPYPS
                     AEGLHVGNMYAFTGSDIYGRFTRMQGHDVFEPIGLDGFGIHSENYAIKIGAHPMKQAK
                     KTEKNFYRQLLMIGNGFAWDNRLETYDPEYYRWTQWIFTELFKAGLAFRKKSPVNWCP
                     SCKTVLADEQVISGKCERCDSEVVKKDLEQ"
     gene            complement(598..1413)
                     /locus_tag="PKG74_00965"
     CDS             complement(598..1413)
                     /locus_tag="PKG74_00965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOB89887.1"
                     /translation="MKPLFFDSHTHVQFVAFKDDHEEVIRRALDKGVWLNNVGSQKET
                     SEKAVMIAEDHEKGVYATVGFHPIHAEAAEKEDLEESVSKSEEFDYDFFCRLAQNPKV
                     VAIGECGLDYFHLKNAAKDKQKEIFLAQIKLAEETSKPLMVHCRDAYPDLIEILRSER
                     SRLLPDRPGISHFFTGSLQDAETLLKMGFSFSFGGAITFAGNYDEVLRRIPLDRLLLE
                     TDAPYVAPVPFRGQRNEPSFVVHVAEHIAKVKSLSLEEVARETTKNALRIFAI"
     gene            complement(1410..2627)
                     /locus_tag="PKG74_00970"
     CDS             complement(1410..2627)
                     /locus_tag="PKG74_00970"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-like YCDxxxxGGGW domain-containing
                     protein"
                     /protein_id="HOB89888.1"
                     /translation="MSRKSFTLIEVLITVSIIAVLSATVVMYLNPVEFLSQGRDAQRI
                     ADIERLNTAVSLYDLKVRGSMGQANKIYISLPDTTASCPTHISFLPEIPGYTYNCATA
                     ANLKKTNGTGWLPVNLDNIPGGSTILQLPIDPVNSARDTLYYSYVTDGTQWEISSNIE
                     SRRIGLERASKDGGDNVLAYEAGSRSDLTPLAVLGTSCNNIHSLFPNGASGLYLIDVD
                     SGGVEPAFPVYCDMTTDGGGWTLVQTTIKGQPVDTRWALPFTSQLDQTIGVPSLDSPY
                     RLAMKYWYMIPNTSWSKMAITTAEGKATFNKSATFSLTGINGPQTGFTYSGSDPAAAL
                     NSLSSYNWNTCTNGLAYFNTSCCGTCILYNSPTPYNAYNQPMMSTVTAIDGTAIQKWS
                     GHAPLDRLNIFSR"
     gene            complement(2634..4091)
                     /locus_tag="PKG74_00975"
     CDS             complement(2634..4091)
                     /locus_tag="PKG74_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOB89889.1"
                     /translation="MKKRKKFYITTSIAYANASPHIGFALELVEADAIARYRRSFGDD
                     VFFLTGTDEHGEKISRVVCSSGMTPKAFTNKISKEFRSLKTELNLSPDDFIRTSDKKR
                     HWPGALALWKRIEENGDIYKKKYKGLYCVGCEKFITKKELVDGRCPIHKTEPEIIEEE
                     NYFFKLSNYRDEVYKLIESGKYLILPESRKNEVLSFLSTGLEDISFSRSRENLSWGIP
                     IPKSDQTMYVWCDALSNYISALGFGRKGGSLRRFNKFWPADIHLIGKDILRFHAVIWP
                     AMLMSAKLPLPKKLLVHGFITAGGVKMSKSLGNVVYPGEIVRKYGVDPTRYYLLREIP
                     SNEDGDFSEEKFVERYTADLANGLGNFASRVLNLGAESAFRGERISEATELQIKRAKA
                     MVDKHMEEFRIHEAMASIWELLKFGDGYTNETKPWKTKDKKVIFDLLMILENAAGLLE
                     PFLPETSKKIKKCFRRRGGYLRVSKIKPLFPRIES"
     gene            complement(4092..5117)
                     /locus_tag="PKG74_00980"
     CDS             complement(4092..5117)
                     /locus_tag="PKG74_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOB89890.1"
                     /translation="MEKRELEISWGTLWRVVATIAAVAFLYAAHEALLVLVFALIISS
                     ALDGLVSWLEERKIPRLLGTIFIFLAVSAVLVFILYEVVPLIFWETAKLVESYQGIGD
                     FFGENSVFLEGMNKTLMTEDFGSRVVDAILEGASPFISAVGGLLGGVVFLATVFIASF
                     YLTLSRDGVERLLRVVFPAQAEEYIIKIYLRVKKRIGRWMQVQMILSLVIGLMVFAGL
                     WLMGVDYSLLIGVLAGLFEIVPMVGPILAGAIAVAVAFSESMSLAIIVFVFFVVIQQL
                     ENNILVPVLMRKAVNIHPVIVIIALMAGFEASGVVGAILAVPVAVVVQEIVEDKLRRK
                     AAEASGR"
     gene            complement(5121..>5551)
                     /locus_tag="PKG74_00985"
     CDS             complement(5121..>5551)
                     /locus_tag="PKG74_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89891.1"
                     /translation="AAYYVRSGTDESSDHGSIFVAAGIDRSKTTEVCRAILAEFEDLK
                     INLVSEDELAKAKEHLSGNLILQLETSDDIALFFGSEEIVTGKVLAPAEALRKIKEVT
                     PEDIRSLAREIFQKNRLNLAAIGPDFEEKALRDILQSTKI"
BASE COUNT         1326 a         1419 c         1399 g         1407 t
ORIGIN      
        1 ttgttccaag tcttttttga cgacttcgct gtcgcagcgt tcgcattttc cggaaatcac
       61 ctgctcgtcc gccaaaaccg ttttgcagga cggacaccaa ttcaccggag attttttccg
      121 gaaagccaaa ccggccttga aaagctccgt gaaaatccac tgggtccaac ggtaatattc
      181 cggatcgtag gtctcgagac gattatccca ggcgaatccg ttgccgatca tcaaaagctg
      241 tcggtagaaa ttcttttccg tttttttcgc ttgcttcatc gggtgcgctc cgattttgat
      301 ggcgtagttt tccgaatgga ttccgaagcc gtcgagcccg atcggctcga aaacatcatg
      361 tccttgcatg cgcgtgaacc ggccgtagat gtcgctgccg gtaaaagcgt acatattacc
      421 gacatgtaac ccttcggccg aagggtaagg aaacatcatc aggttgtaaa aaggcttttt
      481 ggcctttttg agatctggct gaaaaacctt ctttttgagc cagaaagcgg tccatttctt
      541 ctcgattttc cgaaacggat aattcttcat acagaagaat gataaggctt ttcaggctta
      601 gattgcaaaa atccgcaggg cgtttttcgt ggtttccctc gccacttctt ccaagctcaa
      661 gcttttgact ttggcgatat gttccgccac gtgaacgaca aaactcggtt cgtttctttg
      721 cccgcggaaa ggaaccggcg ccacgtacgg cgcgtcggtt tcgagaagca gacgatcgag
      781 cgggatgcgc cgcagaacct catcgtaatt tcccgcgaaa gtgatggctc cgccgaaaga
      841 aaaagaaaaa cccatcttaa ggagtgtttc ggcgtcttga aggcttcccg tgaaaaaatg
      901 actgattccc ggccgatccg gaagaagccg cgagcgttcg ctccgaagaa tctcgattag
      961 atcgggataa gcgtcacggc aatgaaccat caaaggctta cttgtttctt ccgccaattt
     1021 gatttgagcc aaaaaaatct ctttctgttt gtctttggcg gcgttcttta agtgaaaata
     1081 atccaaaccg cactcaccta tggctaccac tttcgggttt tgcgcgagcc gacagaaaaa
     1141 atcatagtcg aattcttcgc tttttgaaac cgattcttcc agatcttctt tttccgcggc
     1201 ttcggcgtgg atcggatgga aaccgacagt ggcgtaaact cctttttcgt gatcttcggc
     1261 gatcatcacc gctttttccg aagtctcttt ctgtgagccg acgttattca accacacgcc
     1321 tttatccaga gcgcgccgga ttacttcttc gtgatcgtct ttaaaggcaa cgaactggac
     1381 atgggtgtga gaatcgaaaa aaagaggctt catctcgaaa aaatgtttag tctgtcgaga
     1441 ggggcatgac cgctccattt ttggatggct gttccgtcga tcgcggtgac ggtcgacatc
     1501 atcggctgat tatacgcgtt atacggagtc ggagagttat ataaaataca agtgccgcaa
     1561 caactggtat tgaagtacgc cagaccgttg gtgcaagtgt tccagttata actgcttagg
     1621 gagttaagtg ccgccgccgg gtctgatccc gagtaagtga atccggtctg gggaccatta
     1681 atccccgtaa gggaaaaagt ggctgactta ttaaatgtag ccttgccttc ggcggtggtg
     1741 atggccattt tggaccagct cgtattcggt atcatgtacc aatacttcat cgctaaccta
     1801 tacggggaat caagtgaagg aacgccgatg gtttgatcga gttgggaggt aaacggcagc
     1861 gcccatctcg tatcgacggg ctgcccctta atcgtggttt gcacaagggt ccagcctcct
     1921 ccgtcggtag tcatatcgca ataaaccgga aaagctggtt cgacgccgcc actgtcaaca
     1981 tctatgagat ataatccgct ggctccgttc ggaaaaagag aatggatgtt gttgcagctt
     2041 gttcccagaa ccgccaaagg ggttaagtct gatcgggaac ccgcttcgta ggccaggacg
     2101 ttgtcgccgc cgtctttcga ggctctttca agaccgatcc tcctcgattc gatattcgaa
     2161 gaaatttccc attgcgttcc gtcggtgacg tacgaataat aaagcgtgtc tctcgccgaa
     2221 tttacgggat cgatcggaag ctgcagtatt gtcgaacctc cgggtatatt gtcgagattg
     2281 accggcaacc agccggttcc gttggttttc ttgagattcg ccgcggtggc gcagttataa
     2341 gtatatccgg gaatttccgg cagaaacgaa atatgggtgg gacagcttgc cgtcgtatct
     2401 ggcaacgata tatagatttt gttcgcttgt cccatagagc cccggacttt caagtcataa
     2461 agactaacgg ccgtgttaag tctttcgata tcggcgattc tttgggcgtc gcgcccctgg
     2521 cttaagaatt cgaccgggtt cagatacatg acaaccgtcg ccgaaagcac ggcgatgatg
     2581 gaaacggtaa tgagaacttc gatcagcgta aagctttttc tgctcatttt ttattatgat
     2641 tctatcctcg ggaaaagcgg tttgattttg gaaacgcgaa gatatcctcc tcgccgacgg
     2701 aagcattttt ttattttttt cgacgtctcc ggtagaaacg gttcgagcag tccggccgcg
     2761 ttttccaaga tcatcagcaa atcgaagatg actttcttgt ctttagtttt ccagggcttg
     2821 gtttcgttcg tgtagccgtc gccgaatttc aggagctccc agatcgaagc catcgcctcg
     2881 tggattctga attcttccat gtgtttatcc accatagcct tggccctttt tatctgaagt
     2941 tccgtcgcct cggatatacg ctctccgcgg aaagccgatt ccgctccgag gttcaaaacc
     3001 ctcgaagcga aattgccgag cccgttcgca agatcggccg tgtagcgctc gacgaatttt
     3061 tcttcgctga aatcaccgtc ctcgttcgaa gggatttccc gcaagagata ataccgcgtc
     3121 ggatcgacgc cgtattttct cacgatctct ccgggataaa cgacgttgcc gaggcttttc
     3181 gacatcttga ctcctcccgc ggtgatgaat ccgtggacca ggagtttttt cggcaaagga
     3241 agcttggcgg acataagcat cgccggccag atgacggcgt ggaatctcaa gatatccttg
     3301 ccgatcagat ggatatcggc cggccaaaac ttgttgaatc ggcggagact accgcctttc
     3361 cggccgaaac cgagggccga gatgtaattg gaaagcgcgt cgcaccagac gtacattgtc
     3421 tggtcgcttt tcgggatcgg gattccccag gaaagattct cccgcgaacg gctgaagctt
     3481 atgtcttcga gcccggtgga gagaaacgac aaaacctcgt ttttacgcga ctctggcaaa
     3541 atcagatatt tgccgctctc gataagcttg taaacttcat cgcggtagtt cgacaacttg
     3601 aagaaataat tctcttcttc gatgatctcg ggttcggttt tgtggatcgg gcagcgtccg
     3661 tccaccaatt cttttttggt gatgaatttt tcgcagccaa cgcaataaag ccctttgtat
     3721 tttttcttgt aaatgtctcc gttttcttcg atccgcttcc aaagagccaa ggctccgggc
     3781 caatgacgct tcttgtccga agtcctgatg aaatcgtcag gagaaagatt gagttcggtt
     3841 ttgagcgacc ggaattcctt ggaaatcttg ttggtaaaag ctttgggagt cattcctgaa
     3901 gaacaaacca cgcggctgat cttttcgccg tgttcgtcgg ttccggttag aaagaaaacg
     3961 tcgtcgccga agcttcgtcg gtatctcgca atggcatcgg cttccaccag ctccaaagcg
     4021 aacccgatat gcggagacgc gttcgcgtaa gcgatcgacg tcgtgatata aaatttcttt
     4081 cttttcttca tttaacggcc ggacgcctcc gcggccttgc gccgcagctt atcctcgacg
     4141 atttcctgaa caacgaccgc cactggcacc gccaggatgg cgccgacaac gccggacgct
     4201 tcgaatccgg ccatcaaggc gataatgacg atcaccggat ggatgttcac ggccttgcgc
     4261 atcagaacag gaacaagaat gttattctcg agctgctgga tgaccacgaa aaagacaaag
     4321 acgatgatgg cgagagacat ggactcggaa aaggcgacgg ccacggcaat ggctccggcc
     4381 agaatcggac cgaccatggg cacgatttcg aaaagcccgg ccaacactcc tatcaaaaga
     4441 ctgtaatcga cgcccattag ccaaagcccc gcgaaaacca tgaggccgat gacgagactc
     4501 aaaatcattt ggacctgcat ccagcggccg atcctctttt tgacgcggag atagatttta
     4561 atgatgtatt cttcggcctg ggccgggaaa acgactcgca aaagtctttc cactccgtcg
     4621 cggctcaagg tgagataaaa agaagcgata aaaaccgtcg ccagaaagac gactccgccg
     4681 agcaagccgc cgacggccga aataaagggg ctcgcgcctt ccaagatcgc gtccaccacc
     4741 cggctgccga aatcctcggt catcagggtt ttgttcattc cttccagaaa aacgctattc
     4801 tccccaaaaa agtcgccgat tccctggtaa gactcgacca gtttggctgt ctcccagaag
     4861 atcaacggga cgacttcgta caatatgaaa acaagaacgg ccgataccgc gagaaaaatg
     4921 aaaatcgttc cgagaagacg cgggattttc ctttcttcga gccaggagac caatccgtcg
     4981 agggctgaag aaatgatgag cgcgaaaacg agcacgagta gagcttcgtg cgccgcgtac
     5041 aaaaaggcga cggcggcgat cgtcgccacc actctccaga gagtgcccca ggaaatttcg
     5101 agttcccgtt tttccattga ttaaatctta gtggattgca ggatatcccg caatgccttt
     5161 tcttcgaaat ccggaccgat ggcggccagg ttcaaacgat ttttttggaa aatttcccga
     5221 gccaaagaac gaatatcttc gggagtcact tctttgatct tccgtagggc ctcggccgga
     5281 gcgagaacct tgccggttac tatttcttcg ctaccgaaga aaagggcgat gtcgtccgac
     5341 gtttcgagct gtaggatcaa gtttcccgat agatgctctt tggccttggc gagctcgtcc
     5401 tcggaaacca aattgatttt caggtcctcg aattcggcga gaatggctcg gcagacctcg
     5461 gttgttttcg atctgtcgat gccggcggcg acgaaaatcg aaccgtggtc ggatgattcg
     5521 tcggttccgc tcctcacgta ataggcggct c
//
LOCUS       DAOPQT010000025         5535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_16101_length_5535_cov_3.554380, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000025 DAOPQT010000000
VERSION     DAOPQT010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16101_length_5535_cov_3.554380"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PKG74_00990"
     CDS             complement(<1..848)
                     /locus_tag="PKG74_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOB89892.1"
                     /translation="MSDINHKKTIFPNGLRYIFIPRPQSLATTVLVLVAAGSEYETKD
                     ISGVSHFLEHLCFKGTTKRPKSGLIASELDALGARYNAFTANEMTGYFAKAANKNFER
                     ILEIVADLYLHPTIDQGEMDKERGVILEEINMYEDLPMEKVKENFSELLYGDQPAGWS
                     VAGDKEVIRKIKREDVVAYRGKHYLASKTTVVVCGGIEEDPEKIVWKHFESMPRGEAL
                     KKEKTVDEQSEPQIDLRTKQTDQFHLVLGSRGIDIFDPRRYALALIAEILGGGMSSRM
                     FVKIRD"
     gene            complement(852..2192)
                     /locus_tag="PKG74_00995"
     CDS             complement(852..2192)
                     /locus_tag="PKG74_00995"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOB89893.1"
                     /translation="MEIDLMDKILSLAKRRGFVFPGSEIYGGLQNSWDFGPLGVELKR
                     NIKAAWWDRFIRRRPDMVGLDASILMNTKVWEASGHLKKFSDPLVECVKCHKRFRGDR
                     IKTEDGCPECGGKLGEAKQFNMMLKTRLGATEDKASEVYFRPETAQGIFVNFKNIMDT
                     ERMSLPFGVGQIGKAFRNEITPGNFIFRTREFEQMEIEYFIPVPEKDKDWNNYFEYWR
                     KEMLEWISSLGVDVKKEIRELEVPEKDRAHYSKRTVDFEYAFPFGEEELYGLAYRGDF
                     DLKNHGETSGADLKYKDPVSGEEFYPHVIEPTFGVERSILAAMCSAYTEESAPTAEKG
                     EEETRVVMKFPKWLAPVKAAVLPLSKKEELSSVAKKLADDLRNDLFVDYDETQSIGKR
                     YRRQDEIGTPYCVTIDFDSLEDKKATIRDRDTMKQERVAIAEIKSYLLERFKEQ"
     gene            complement(2214..3905)
                     /locus_tag="PKG74_01000"
     CDS             complement(2214..3905)
                     /locus_tag="PKG74_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89894.1"
                     /translation="MRKEKIRILTLVKWILFAAVGIAAAFVVYELFFAGSARRPAISF
                     EAPPNVVLGEDFPLTVVFVNNSEVLIRNVEISMTVPNGAMFSDGKNEVRRMREAGTMD
                     VGEEYKETFNIRVLDAAEAEKSFEASVSYLPATLNKSLIVSKDFIVSVETPVKTELVV
                     PNEVMAGEEFNWSLNYENTSDQEWILLFNLTAPKDFSSNFSESPITVKPGEKGEVSFK
                     GSANLPDMSVFSLRVETRGELNGRDYVVSDVSKDIAIKASPLSLKIDLLNAESSSGAV
                     KPGSILRYRLVCSNFSNEVFRNVIVRASLKGNMYEFAGLAVSGNASLNNGTIFWNYAT
                     DPDLAALDPGESREIIAEVPLKSDYAVRQASDKDFTGTMTANAEAVVASTGIKLTSVV
                     VFDAKLVGVLKISPKIYFRDAASGIINSGNLPLKVGSPTDFTVHWALSNQASDMSNIE
                     VRAVLPVGVSLTGNQKIPVGEFSLDPNSNEVVWKIPKMLANTGVVDKPLEAIFQIRAT
                     PIDEFTGSYMPLLRETRATALDDFAGVSVSATGLPLNSFLTDDPTVSPLDGLIRG"
     gene            complement(3878..4393)
                     /locus_tag="PKG74_01005"
     CDS             complement(3878..4393)
                     /locus_tag="PKG74_01005"
                     /inference="COORDINATES: protein motif:HMM:NF023393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HOB89895.1"
                     /translation="MVEYLTEAIILDKRPDKNLDAVFTVFSRNLGKVYGKATSSRKIT
                     SKLASHLEPGTLAKIRLARKSVGDNFRIVEALAEAKRSDPETIRFLMFLDKMVPASAK
                     DEVLFMFCREVIKDPEFSEKMAYRGLLKIIGLDPDKACCAVCGSRKIAYFSSQDIMFL
                     CRQCGKKRYGY"
     gene            4543..4989
                     /locus_tag="PKG74_01010"
     CDS             4543..4989
                     /locus_tag="PKG74_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89896.1"
                     /translation="MGKFLLGTMLMSLVVSAQGRLTDVAANTAPNTPDHPYVVLGGDS
                     LLASNEPASTRSIVTTITAYSSTPDQTDDTPFITASGKFVGDGIVAANWLPFGTKIRI
                     PEIFGDKVFEVQDRMAPKHDEKLDIWFKTREEALKFGVRKSRIEIL"
     gene            complement(5053..5295)
                     /locus_tag="PKG74_01015"
     CDS             complement(5053..5295)
                     /locus_tag="PKG74_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89897.1"
                     /translation="MEKQILKLRDDQIMALFWLVGVRFEKKYFEDIIKEIRTNKEDSQ
                     YLGILIDEADSKNNLSVWISYFEKENKKNRFWNIFR"
BASE COUNT         1294 a         1462 c         1303 g         1476 t
ORIGIN      
        1 tcgtccctga ttttcacgaa catcctcgaa gacatgccgc cgcccaaaat ctcggcgata
       61 agcgccaagg cgtatctccg cggatcgaaa atatctatcc cccggcttcc gagcacgagg
      121 tggaactgat cggtctgttt tgttcggaga tcgatttgcg gttctgattg ctcgtcgacg
      181 gttttctctt ttttcaaggc ttcgccccgg ggcatggatt cgaaatgctt ccagacgatc
      241 ttctccggat cttcctcgat ccctccgcag acgaccaccg ttgttttcga agcgagataa
      301 tgtttacctc tgtaagccac gacgtcttcc cttttgatct tccggatcac ttctttgtct
      361 ccggcgacag accagccggc cggctggtct ccgtaaagga gttcggaaaa attctctttg
      421 actttttcca tcggcagatc ttcgtacatg tttatttctt cgagaatcac tcccctttcc
      481 ttgtccattt ctccctggtc gatcgtcgga tggagataaa ggtcggccac gatctcgaga
      541 atccgctcga aattcttgtt ggccgccttg gcgaaatatc cggtcatttc gttggcagtg
      601 aaagcgttgt atctggcgcc caaggcgtca agttcggaag cgatcaatcc gcttttcgga
      661 cgcttggtcg ttcctttaaa gcaaagatgc tccaaaaagt gggaaacgcc gcttatgtct
      721 ttggtctcgt attccgatcc ggcggcgacg agaaccaaaa ccgtggtcgc caggctttgc
      781 ggccggggaa tgaatatgta tctcaaacca ttcgggaaaa tggtcttttt atgattgatg
      841 tcggacatga tttactgttc tttgaaccgc tccaaaagat aactcttgat ttcggcaatg
      901 gcaacccttt cctgcttcat ggtgtcgcgg tcgcggatgg tcgccttctt atcctccaag
      961 gaatcgaagt cgatagtgac gcaatacggc gtgccgatct cgtcttgccg gcggtagcgc
     1021 ttgccgatcg attgggtttc gtcgtaatcg acgaaaagat catttcttaa atcgtcggct
     1081 aatttcttcg ccaccgaaga caattcttct tttttggata aaggaagcac ggccgctttc
     1141 accggagcga gccatttcgg gaatttcata accactcgcg tctcctcttc tcctttttcc
     1201 gccgttggcg ccgattcttc ggtataagcc gaacacatgg ccgccaaaat acttctttcg
     1261 actccgaaag tgggttcgat gacgtgcgga tagaactctt caccggaaac cggatctttg
     1321 tatttcaaat cggctccgga agtctcgccg tgatttttaa gatcgaaatc tccgcgataa
     1381 gccaaaccgt aaagctcctc ttctccgaaa ggaaaagcgt attcgaaatc gacggtccgc
     1441 tttgaataat gggcgcggtc tttttccggc acttcgagtt cacggatttc ttttttgacg
     1501 tcaacgccga gcgaagaaat ccactcgagc atttctttcc gccagtattc gaaataattg
     1561 ttccagtctt tgtctttttc ggggaccgga ataaaatact ctatttccat ttgttcgaat
     1621 tcgcgcgtgc ggaaaataaa gtttcccgga gtgatttcgt tccggaaagc cttgccgatt
     1681 tggccgactc cgaaaggcag gctcatccgc tccgtgtcca tgatgttttt gaaattgacg
     1741 aatatgccct gggccgtttc cggacggaaa taaacctcgg aggctttgtc ttcggtcgcg
     1801 ccgaggcggg ttttcagcat catattgaat tgcttggctt ctccgagttt gcccccacat
     1861 tcgggacagc cgtcttctgt ctttatccgg tcgccgcgga accgcttgtg gcatttaacg
     1921 cattcgacga gcgggtcgga aaactttttc aaatgcccgg aagcttccca aaccttggta
     1981 ttcatgagaa tcgaagcgtc gaggccgacc atgtccggac gcctgcggat aaacctatcc
     2041 caccaggcgg ctttgatgtt tcttttgagc tccactccca aaggcccgaa atcccacgag
     2101 ttttggaggc cgccgtagat ttcgcttccc ggaaaaacaa aaccccggcg tttggccagg
     2161 cttaagattt tatccatcaa atcaatctcc atgatcttct tatatatatg atattatccc
     2221 cggatcaacc cgtctaacgg actgaccgtc ggatcatcgg tcagaaagct gttcaaggga
     2281 aggcccgtgg ccgacaccga gactccggcg aaatcgtcta aagccgtcgc cctcgtctcc
     2341 cggagaagcg gcatgtacga accggtgaat tcgtctatcg gcgtcgctct tatctggaag
     2401 atcgcctcga gcggtttgtc gacaacgccg gtattggcga gcatcttcgg tattttccag
     2461 acgacttcgt tggaattcgg gtcgaggctg aactcaccga ccggaatttt ctggttgccg
     2521 gttaaactca cgccgaccgg caaaacagcc cgcacctcga tattgctcat gtcggaggcc
     2581 tgattcgata gcgcccaatg gacggtaaag tctgttgggc ttcccacttt caagggaagg
     2641 ttgccgctat tgatgattcc cgaagccgcg tcgcggaagt agatcttcgg actgattttc
     2701 aaaactccga ccagcttggc gtcgaaaaca acgacgctcg tcaattttat tccggtcgaa
     2761 gccactacgg cttcggcgtt cgccgtcatc gttcccgtga aatctttgtc gctcgcctgc
     2821 ctcaccgcgt aatcggattt aagcggaacc tccgcgatga tctccctcga ttcgcccgga
     2881 tccaaagccg cgagatcggg gtcggtcgcg taattccaaa aaatcgttcc gttgttcaag
     2941 ctggcgtttc cggataccgc aagaccggca aactcgtaca tgtttccttt gaggcttgcc
     3001 cggacaatga cgttcctgaa aacttcgttg ctgaagttgg agcagacgag gcggtatctc
     3061 aaaatacttc cgggcttgac cgctccggac gaggattcgg cattcaaaag gtctattttc
     3121 aaagaaagcg gcgatgcctt gatggcgata tccttggaaa cgtcgcttac gacgtaatcg
     3181 cgaccgttca attctcctct ggtttcgacg cgcaggctaa aaacactcat gtccggcaga
     3241 ttggccgagc ccttgaaaga gacttctcct ttttctccgg gcttaaccgt gatcggagat
     3301 tccgagaaat tactcgagaa atccttgggc gcggtcaggt tgaaaagcag aatccattct
     3361 tggtcgctag tattctcgta gttcaaggac cagttgaatt cttcgccggc catgacttcg
     3421 ttcggaacga caagctcggt tttgaccggc gtttccacgc tcacgatgaa atccttgctg
     3481 acgatcaggc tcttgttcaa agtcgccggc agataagaaa cactcgcctc gaagctcttc
     3541 tcggcttcgg ccgcatcaag cactcttata ttgaaggttt ctttatactc ctcgcccacg
     3601 tccatggtcc cggcttcgcg catccttctc acttcgtttt ttccgtcgct aaacatggcg
     3661 ccgttcggaa cggtcatcga tatctccacg tttctgatca aaacctcgga attattgacg
     3721 aaaacgaccg tcagcggaaa atcttctccg agaacgacgt ttggcggagc ctcgaaagaa
     3781 atggccggcc ttctcgcgga tccggcgaaa aacagctcgt aaacgacaaa agcggccgcg
     3841 ataccgacgg ccgcgaagag aatccatttc actaaggtca atatccgtat cttttctttc
     3901 cgcattgacg gcataaaaac attatatcct gactggaaaa ataggcaatt tttctgcttc
     3961 cgcacacggc gcaacacgcc ttgtccggat caagaccgat tattttcaaa agcccgcggt
     4021 aggccatctt ttccgaaaac tccgggtctt tgatcacttc ccggcagaac atgaaaagca
     4081 cctcgtcttt ggccgaagcc ggaaccatct tgtcgagaaa cataaggaat ctgatcgtct
     4141 ccggatcaga ccgcttggct tcggccaggg cctcgactat ccggaagttg tcgccgacgc
     4201 ttttcctcgc caatctgatt ttggcgagcg ttccgggctc gagatggctc gccagcttcg
     4261 aagtgatctt ccggctgctc gtcgcttttc cgtaaacctt gccgagattc cggctgaaaa
     4321 ccgtgaaaac agcgtccaga ttcttgtccg gccgcttgtc aaggattatg gcttcggtca
     4381 gatactcaac cataagccta ttataggctt tggccgccgg ccggaaaacc ccgaatttgg
     4441 ctagatttca agcgtttttc cgccatcttg acaaaacagg gctaatcggc taagcttaaa
     4501 gagtaataaa tttcccctgt gagggggttt tttattacga caatgggtaa attcctattg
     4561 ggaacgatgc tgatgagtct ggtcgtgtcc gctcagggac gtctaaccga cgtcgcggcg
     4621 aacactgctc ctaatactcc ggaccatccg tacgtcgttc tcggaggcga ttcgctctta
     4681 gcttcaaatg agcctgcttc gactaggtcg atcgtgacga caatcaccgc ctactccagc
     4741 actcctgacc agaccgatga tactccgttt atcaccgcct ctgggaagtt tgtcggagac
     4801 ggaattgttg ccgcgaactg gttgcccttc ggaaccaaaa tccgtatccc ggaaatcttc
     4861 ggagacaaag tcttcgaggt ccaggataga atggctccga aacacgatga gaaattagac
     4921 atttggttta aaacaagaga agaggctctc aagtttggag ttagaaagag taggatcgaa
     4981 atcctctaaa caaattgcga ttgtaaataa aacctcccgc cgattggtgg gaggttttat
     5041 tttatccgcg gcttacctga aaatattcca aaaccgattt ttcttatttt ctttttcgaa
     5101 ataagaaatc cacaccgaaa gattattttt tgaatccgct tcatcgatca gaataccgag
     5161 atactgagaa tcttctttat tggttctaat ctcctttatg atgtcttcaa aatacttttt
     5221 ctcgaaccta acccccacca gccaaaaaag cgccatgatt tgatcgtccc gcagttttaa
     5281 aatttgtttt tccataaaat aaagttattt gatgatccgc acgatcacaa atacgtattc
     5341 ttatatatca tataacttat ccaaataaaa aaggaaaaga cccgttggga cgagtctaat
     5401 cttgcagtta ttgaacgaag gaggagatcc ttttcctgtg gggaccccta gaaacacagt
     5461 atagtaagaa aggaagtgat ttcctccttc gtcctgatag gaaggaccaa acgagacagg
     5521 tgctcggaga tcgga
//
LOCUS       DAOPQT010000026         5436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_16467_length_5436_cov_3.339714, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000026 DAOPQT010000000
VERSION     DAOPQT010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16467_length_5436_cov_3.339714"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKG74_01020"
     CDS             <1..242
                     /locus_tag="PKG74_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89898.1"
                     /translation="IVKGDDLSKCLPKMDKTKLNLIVLNGGDDNPGLYGFPDGKIGVV
                     IRENKSQAKTKSKNRRKSVKPKPVKKIKNSRKKPQ"
     gene            264..1160
                     /locus_tag="PKG74_01025"
     CDS             264..1160
                     /locus_tag="PKG74_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89899.1"
                     /translation="MKRKAIFIVLLILAVAALAAAIFVEKPQDRQAAEDVVKTFGEKL
                     RNVSLLESQDIFAESVQENYGELVSARLIDKWISDPKSAPGRLLSNPWPHHIEIRSIK
                     KSFINSYKISGDIIEVTEAELGETVLLIKQPVEIVVGKTNKGWRINDFKASAYKTVLP
                     RNIPDNIASTFSFDYPAILAAEFIYPEIWPPILTFSDEEYSCAITAAAPDLPERVAEK
                     VIDARAYCVKTLDEFDDDSIQTTYTYSALRDNRVVNLEFALRYPRCENLNEPERTECS
                     RERLGFDLNAIIASIMTSLRNQ"
     gene            1424..2197
                     /locus_tag="PKG74_01030"
     CDS             1424..2197
                     /locus_tag="PKG74_01030"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HOB89900.1"
                     /translation="MKKKYSKITFLALAVLAISLIACFLYKGLLSGKALTLENHNVSE
                     NNDFFRIRAEYPQFPGASRDFNNKIKDLIAGKISEFKNNSNEYWKARRETALPGETVP
                     TAPEVPFEFIAQWKHDRLDDRYISVVLNIYYFSGGAHGNEEIYTFNYDLKNRREIGIM
                     DFVGSEENLQEISNLAKAQLSASLESMGWRSTSDTWSMLDEGAAPTAENYKDFTFNRD
                     VLTVYFQKYQVAAGAAGSQQTSFYKDNLDATGIDLNWPN"
     gene            2229..2462
                     /locus_tag="PKG74_01035"
     CDS             2229..2462
                     /locus_tag="PKG74_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89901.1"
                     /translation="MTLSNFGLLMIMLGWVWQLAAVMKGKRQIQLGFLLLYAIGVLML
                     IWNRLGSGSLSLSDWLELADIVIVVILMFKLKK"
     gene            2480..2920
                     /locus_tag="PKG74_01040"
     CDS             2480..2920
                     /locus_tag="PKG74_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011623549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOB89902.1"
                     /translation="MDQFMEAALREAESGIEEGGIPIGSVLVKDNRIIGQGHNRRVQK
                     GSSILHAEMDCLESAGRLKPEDYARSVLYSTLSPCDMCAGAILLYKIPKVVIGENENF
                     KGPEEYLRSRGVEMTDLDLEECKKMMRDFIKEKPELWNEDISEE"
     gene            2936..3136
                     /locus_tag="PKG74_01045"
     CDS             2936..3136
                     /locus_tag="PKG74_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89903.1"
                     /translation="MNKKQSKIILIVLIVILVAAAIFAYYSCRRKNEAVFCTLDAKLC
                     SDGSYVGRVAPDCEFASCPGEE"
     gene            3521..3787
                     /locus_tag="PKG74_01050"
     CDS             3521..3787
                     /locus_tag="PKG74_01050"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOB89904.1"
                     /translation="MRKDFKLKVFEVVKAIPKGETLSYKRVAELAGRPKAYRAVGNIL
                     SRNYDPSIPCHRVIRSDEAPGGYNRGSVAKIRKLREEGVNQFRG"
     gene            complement(3772..4329)
                     /locus_tag="PKG74_01055"
     CDS             complement(3772..4329)
                     /locus_tag="PKG74_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOB89905.1"
                     /translation="MALKPKILLHVCCAVCGTLMVKILRERFEPIVFYSNSNIHPEEE
                     YRKRLEAVKKLASENQTEFIEDRYDPPAWYEAVSGLEKEPEGGKRCPVCFALRLGKTA
                     AEAKRLDIRFFTTTLSASPHKNEHDIDRLGKMIAEETGTEFVAFSEICSDKKDSWRKT
                     SEMSRKAGFYRQKYCGCEFSNQPRN"
     gene            4384..5172
                     /locus_tag="PKG74_01060"
     CDS             4384..5172
                     /locus_tag="PKG74_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOB89906.1"
                     /translation="MDFRQTRFLSIVSVFMMAAAAFFLVSENNRQASKIKFGFSGFKE
                     TIPPDIILSEEKTIALPLVVPEYIQKYRASCEVAATRAALLYFGVVFSEEDMIEEIGW
                     EILPRYYDKNGDLVWGNPQSKYVGHYDPERIYIDGYGVYNQPIYGFLSEHGFGKSISK
                     TNWSIDEIISYVRQGYPVIAWTSGDFKKRVPGVMISPDGAKNPWIFSEHAVVVRGFDE
                     KRVYLMDPAPRAGYRQVTWKEFEDGFGSLNNMAIAIMPGEMAEL"
BASE COUNT         1476 a         1200 c         1333 g         1427 t
ORIGIN      
        1 cgatcgtcaa aggcgacgat ctatccaaat gcctaccgaa gatggataaa accaaattaa
       61 accttattgt tctaaacgga ggagacgata atcccggcct ttacggattt cccgacggga
      121 aaataggagt ggttataaga gaaaacaaaa gccaagccaa aacgaaatcg aaaaatcgtc
      181 ggaaatccgt aaaaccaaag ccggttaaaa agatcaaaaa tagccgaaag aagccccaat
      241 gatttttatg ttaatctaat attatgaaaa ggaaagcaat ttttatcgtt ctccttattc
      301 ttgcggttgc ggctttagcc gcagcgatat tcgtggagaa accgcaagat cgtcaagcgg
      361 cggaggacgt cgtaaaaact tttggagaaa aattaagaaa cgtttccttg ctcgagtcgc
      421 aagatatttt tgccgagagc gttcaagaaa actacggaga actggtctcg gccagattga
      481 tcgataaatg gataagcgat ccgaagagcg cgccgggcag gctgctttct aatccctggc
      541 cccatcatat cgaaataaga tcgatcaaaa aatcctttat caattcttac aagataagcg
      601 gagacatcat cgaggtcacg gaagccgaat tgggcgaaac cgttttgctt atcaagcagc
      661 cggtggagat cgttgtcggg aaaacgaata agggctggcg gatcaatgat tttaaggcgt
      721 ctgcctataa aacggtcctt ccgaggaata tacccgacaa catcgcgagc actttcagct
      781 ttgactatcc cgcgattttg gctgccgaat tcatctatcc cgaaatatgg ccgccgatat
      841 tgaccttcag cgacgaggag tattcctgcg ccataaccgc cgccgcgccc gacctgccgg
      901 aacgcgtcgc ggaaaaagtg atcgatgcca gagcttattg cgtcaagact ctggacgaat
      961 tcgacgacga ctcgattcag accacctata cttattccgc tttgcgggac aatagagtcg
     1021 tcaatctcga gtttgccttg cgatatccgc gatgtgaaaa cctcaatgag ccggaaagaa
     1081 ccgaatgttc gcgcgaacgg ctgggttttg acctgaatgc gattatcgcc tcgattatga
     1141 cgagcttgag gaatcaataa tttaaattaa ccccgaagtt atccacaggg aaggtattga
     1201 aggattctgg gcgcttaata ctatttaaat agctgatgaa gttggcgtta gtcagcttat
     1261 acttacagat catggttcgc ccagaaactc gagcctaaaa aacttggggc tgtaagcgaa
     1321 gaatacgacg gtgccaacga ctaatttaat ttagaaccgt cgtgtttttt gttttttaga
     1381 ataaaatact ggcggcgaaa cacaaccgtg ttataattta tccatgaaga aaaaatattc
     1441 aaaaataacg tttctcgcct tggcggtctt ggcgatcagc ttgatagcct gctttttata
     1501 taaagggctt ctgtcgggaa aggccctgac tcttgagaat cacaatgttt ccgaaaacaa
     1561 cgattttttc cgtatccggg cggaatatcc gcagttcccc ggagcgagcc gagatttcaa
     1621 caacaaaatc aaagatctca ttgccgggaa aatatccgag tttaaaaata actcgaacga
     1681 atattggaaa gcgaggcggg aaaccgcttt gcccggagaa accgtaccga cggctccgga
     1741 agtgcctttc gagtttatcg cccagtggaa gcatgatcgg cttgacgaca ggtatatcag
     1801 cgtcgttttg aatatctatt atttttccgg cggcgctcac ggaaacgaag agatttacac
     1861 ctttaattac gatctgaaga atcgtcggga aatcggcatc atggattttg tcggttccga
     1921 agaaaacctt caagagattt ccaaccttgc caaagcgcaa ttgtcggcaa gcttggagtc
     1981 catgggttgg agaagcacca gcgacacctg gtctatgctc gacgaagggg cggctccaac
     2041 cgcagagaac tacaaagact tcaccttcaa tcgggatgtt ctcaccgtgt atttccagaa
     2101 ataccaagtg gcggccggag cagcgggaag ccaacagacg tctttctaca aagacaatct
     2161 tgatgccacc ggaatcgatt tgaattggcc gaactaaata accaaaggtc ggaattttcc
     2221 atttcacaat gactctttcc aattttggac tattgatgat catgctcggc tgggtatggc
     2281 aactcgcggc cgtcatgaaa ggaaaacggc aaatccaact gggatttttg cttttatacg
     2341 ccatcggtgt cttgatgctt atctggaaca gattgggttc cggatcgctt agcctaagcg
     2401 actggctgga gctcgccgac atcgttatcg tcgtcatctt gatgttcaag ctgaaaaaat
     2461 aattcaatcg tcgcgcccca tggatcaatt tatggaagcc gctttgcgag aagcggaatc
     2521 gggaatcgaa gaaggaggga ttcctatcgg ttctgtcctc gtgaaagata atcgaatcat
     2581 cggtcaagga cacaaccgcc gcgttcagaa aggatcgtcc attcttcacg cggaaatgga
     2641 ttgtctcgaa tccgccggac gactgaaacc cgaagattac gcgagaagcg ttttgtattc
     2701 gactctctct ccctgcgaca tgtgcgccgg agcgatcctt ctttacaaga tccctaaagt
     2761 cgtcatcgga gagaacgaga atttcaaggg accggaggaa tatctgcgtt cccgcggagt
     2821 cgaaatgacg gatctcgatc tcgaagaatg caaaaaaatg atgagggatt tcataaaaga
     2881 aaagcccgaa ttgtggaacg aagatatcag cgaagaataa tcatcgttta taaatatgaa
     2941 taaaaaacaa tcgaaaataa ttttgatcgt cttgatcgtc attctcgttg ccgccgcgat
     3001 cttcgcctat tattcctgtc gccggaaaaa cgaggcggtt ttctgcacct tggacgccaa
     3061 actttgttcg gacggcagtt atgtcggtcg ggtggcgccg gattgcgaat tcgcgtcctg
     3121 ccccggagaa gaataaattt gtccgtttcc gtctttgtca cgacggaggc ttttagggaa
     3181 aaatgatata attgaaatat gactagatta acaatcatcc agctcgtcgt cctggtcgcc
     3241 ggaacgattt ttgcctgggg taattttata aaagaatttg tcgcttggcg taataaaaaa
     3301 agttgcgagg caggttgcgc tcccggagtc aatcccttta aaacaccctg tttttacgga
     3361 gctttggttt ttgcgatcgc cttggtccta catttgctca taatatggac ttttaatgag
     3421 tgggcttctg gtttctggcc gtgggctatc taggcaatag aaagaaaggc tttattaata
     3481 aagaagcaaa atagatttac gatagtgttt tcagtcgttc gtgcgaaaag actttaaatt
     3541 aaaagttttt gaagtcgtta aagccattcc gaaaggcgag actttgagct acaagcgggt
     3601 ggccgaactc gccggaaggc cgaaggccta tcgggccgtt ggaaatattt tgagccgcaa
     3661 ttacgatccg tcgattcctt gccaccgggt gataagaagc gacgaggctc cgggaggata
     3721 caatcgggga tcggttgcca aaataaggaa actacgcgaa gaaggcgtca atcagtttcg
     3781 aggctgattg ctgaattcgc agccgcagta tttttggcgg tagaatccgg ctttgcggct
     3841 catttccgac gtttttctcc aagaatcttt tttatccgaa cagatttcgg aaaaggcgac
     3901 gaattccgtc ccggtttctt cggcgatcat ttttcccagt cgatcgatat catgctcgtt
     3961 tttatgcggg ctggcgctca aggtggtcgt aaaaaatcgg atatcgaggc gcttggcttc
     4021 ggctgcggtt tttccgagcc gcaaggcgaa gcaaaccgga catctttttc ctccttcggg
     4081 ttctttttcc agtccggaaa ccgcttcgta ccacgccggt ggatcgtatc ggtcttcgat
     4141 aaactccgtt tgattctccg aagcgagctt ttttaccgct tccaggcgtt tccggtattc
     4201 ttcttccgga tggatgttgg aattggaata aaagacgatc ggctcgaatc gttcgcggag
     4261 gatttttacc atcaaggttc cgcaaacggc gcagcaaaca tggaggagga ttttcggttt
     4321 taaagccata ttattaacgc ttactctatt ctttatgata agattaagca attatatcca
     4381 tcgatggatt tccgacaaac gcgatttctt agcatagttt ctgtttttat gatggcggcc
     4441 gccgcctttt ttttggtttc ggagaacaac cgacaagcct cgaaaatcaa attcggtttt
     4501 tccggattca aggaaacgat tccgccggat attattctct ccgaagaaaa aacaatagct
     4561 ttacctttgg tcgttcccga atatatacaa aaatacaggg cttcttgcga agtggcggcc
     4621 acccgggcgg ctttacttta tttcggggtt gttttttccg aagaggacat gatcgaggaa
     4681 atcggctggg agatccttcc ccgatattac gataaaaacg gcgatctcgt ttggggaaat
     4741 ccgcagtcga agtacgtggg ccattacgat cccgagagga tttatatcga cggctacgga
     4801 gtctacaacc agccgattta cggttttctt tccgaacacg gattcggaaa aagcatttcc
     4861 aaaacaaact ggagcataga cgagattatt tcttatgttc gccaaggtta tccggtgatc
     4921 gcctggacct ccggagattt caagaaacgg gttccgggtg tgatgatcag cccggacggc
     4981 gccaaaaatc cgtggatttt ctcggaacac gccgtggtgg tgagaggatt cgacgaaaaa
     5041 agagtttatc ttatggaccc ggctccgaga gcggggtatc gtcaggtgac ctggaaagag
     5101 ttcgaagacg ggttcggcag cctgaacaac atggcgatcg cgatcatgcc cggagaaatg
     5161 gcggaattgt aaaggttttt agccctattt tttatccaca atagtcggtt gcgaacttaa
     5221 cctgttgatg taaactaaaa gtccacgaaa tctggtcctt gaaagggtga gtgttatggg
     5281 tctcgacgct ttgtgtgtgg gctctgtcaa tcattatttc tggtgcgccg agtgcaatag
     5341 atctacgttt gcatcagtct ttccaagaga atcttatgtg gaatttgtct tttctttccc
     5401 tggatctctt gacggcagtt tattcgcgat tgagcc
//
LOCUS       DAOPQT010000027         5401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_16603_length_5401_cov_3.532174, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000027 DAOPQT010000000
VERSION     DAOPQT010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16603_length_5401_cov_3.532174"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1523)
                     /locus_tag="PKG74_01065"
     CDS             complement(120..1523)
                     /locus_tag="PKG74_01065"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOB89907.1"
                     /translation="MEKSDLRKIKDWLWEIPRESKPDMKVPARVFATEKMIEEILEDR
                     SLDQLVNVAGLPGIVGHAVAMPDIHEGYGFPVGGVAAFDAENGIISPGGIGYDINCGV
                     RLLCSEVGLPEIRSRVPELGKAFYQEIPSGVGRAGRLKLSDKELDQILLKGVKRMVEL
                     GYADPEDSLNAEESGYLSTADPKMVSVRAKDRGRDQLGTIGAGNHFVELEEIEEIFDK
                     EKAEKLGLFLNQVVVLIHTGSRGLGHQVATDYIKIMMAAAAKYDIVLKDRELASAPFG
                     SEEGQAYFRAMSAAANFAWANRELVSWEARRAWKSVLGEKTGGLRLVYDVAHNIAKIE
                     EHLVDGKKRKLVVHRKGATRAFPDQPVLVPGSMGTRSYLLSGEEAAMTESFGSSCHGA
                     GRRMSRTEARKTIVAGELKQDLAAKDVVVFAGSIAGLVEEAPEAYKDVDEVIDVIQNA
                     GIASKVAKMRPVAVIKG"
     gene            complement(1527..1934)
                     /locus_tag="PKG74_01070"
     CDS             complement(1527..1934)
                     /locus_tag="PKG74_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOB89908.1"
                     /translation="MPYKILGHTADIRLSVEGKDLEEVFKDALKGMVFIAGPREPEDS
                     KETARKIKISAGDRTGLLVDFLNEVLFGMEKNYEFYTDVDFKELSSTKLVASLKARPA
                     IRFERNIKAVTYHEAEIREKENGFLSTTIVFDI"
     gene            complement(1975..2250)
                     /locus_tag="PKG74_01075"
     CDS             complement(1975..2250)
                     /locus_tag="PKG74_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89909.1"
                     /translation="MDESKNAVERAKIIEAIGLSMAKEIEPQDPEVVILAEKWTDLMD
                     ELAYGNGLVREELKGIDHENINDFRERFGEAVPDDETLDYIRRALVD"
     gene            2294..2845
                     /locus_tag="PKG74_01080"
     CDS             2294..2845
                     /locus_tag="PKG74_01080"
                     /inference="COORDINATES: protein motif:HMM:NF018525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOB89910.1"
                     /translation="MTTIVFIHGYNASPKINWYPEMRSELDKLRIPYRMPVLPGEDNP
                     KIEEWLNIIDKTMKSIKGDVILVGHSLGTRALLLYLDRSRKPIKGVLLVSPFSNDIGN
                     AGVRGLGYSDFFEHKIDLSEIKKLSPKFIVMHSKDDQRIPFEQGKELAAELGADLALY
                     EDKRHFSRPENAVDVLEQLKKLF"
     gene            2845..3237
                     /locus_tag="PKG74_01085"
     CDS             2845..3237
                     /locus_tag="PKG74_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89911.1"
                     /translation="MDIKSAEKLFDENGKQILGIFKKIQMLEFWLKAKIADKKSRSAK
                     DFEIYQKSLEDKTWNFVIDEFLTLYPEKEYLRPELKRAMADRNRFMHAIYTWALFSSK
                     TRVEEQGKRMLDEIEENIGRVLKILLAD"
     gene            3293..4750
                     /locus_tag="PKG74_01090"
     CDS             3293..4750
                     /locus_tag="PKG74_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOB89912.1"
                     /translation="MAKNEVILRFDEVSFRYSALKPILDEASFSVRRGAKITLMGQNG
                     AGKTSIFGLIAGELKPESGQISVTPGATVGLSRQVIPRSELELTVREFFEKAFSAKVY
                     DIEPRIEKILKVVNLEAPFDRKVGSFSGGQQARLLLAFALIQNPDILLLDEPTNNLDS
                     EGIGRLKKFIVEYPKTCVVISHDAGFLNSFTEGVLYLDVFTNKVEQYAGNYLDVVEEI
                     SKRIERERMLNARLEKDIENRKEQANFFGQKGGHMRDVAAKMKRKVEELEENKVEMRR
                     DDRVIRSFAIPTQENISGDIVKISSISSSRNGDLVSKKIPAIVLKKKDRLLLTGPNGI
                     GKSTYLEAIVSGKAKGVALAENVKIGYYRQDFSTLDFEKTTYESLAEVMMNGGEEELR
                     SAAAGFLIDADTLKKKISDLSEGQKGLLTFCRLMLQKPGLLILDEPTNHINFRHIPII
                     ADAVNRYEGPAIIVSHMPDFVSKIKITYTIDLGQI"
     gene            4788..5000
                     /locus_tag="PKG74_01095"
     CDS             4788..5000
                     /locus_tag="PKG74_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89913.1"
                     /translation="MKTKKDILSELIDEVKETLIRIEITTRYVEKKQAETQKDRYLQE
                     LAKLQANKEENEDWLKFLEEELKNTK"
     gene            5117..>5401
                     /locus_tag="PKG74_01100"
     CDS             5117..>5401
                     /locus_tag="PKG74_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89914.1"
                     /translation="MRKERTRKVFVFVILFCFILTSILVFVPKDENKPPTEDPIAEEP
                     VKPAEPVKPVEPDKPDPIKPKMKLLSFSEKGDVEPSKIEIDPKVGAGALLF"
BASE COUNT         1392 a         1408 c         1300 g         1301 t
ORIGIN      
        1 acgcacttgt tgttgcaggt ctcggaccag cgaatgatca gaatcggcga ctcgccgcgt
       61 ttgttttctt tcatgtcaat gtcgaaatta tcgcatcctt cgcgacaatt gggaaattct
      121 taacctttaa tgacggccac cggtctcatt ttcgcgactt tggaggcgat gcccgcgttt
      181 tggatgacgt cgatcacctc atccacgtct ttgtaggctt ccggcgcttc ttcgaccaag
      241 ccggcgatcg aaccggcgaa aacaacgacg tctttggccg cgaggtcttg ttttaattct
      301 ccggcgacga tcgtttttct ggcttcggtg cgggacatgc ggcggccggc tccgtggcag
      361 cttgatccga aactttccgt catggccgcc tcttctccgg agagaagata ggatcttgtt
      421 cccatgcttc ccggaaccaa aaccggctga tcaggaaaag ccctggtcgc gcctttgcgg
      481 tggacaacca attttctctt ttttccgtcc acgagatgct cttcgatttt ggcgatgttg
      541 tgcgccacgt cgtaaacaag cctcaatcct ccggtttttt ctccgagaac gcttttccag
      601 gcccgcctcg cttcccagga gaccaattcg cggttggccc aggcgaaatt ggccgccgcg
      661 gacatggctc ggaaataggc ttgaccttct tcggagccaa agggcgcgga agccaattcc
      721 ctgtctttga gaacgatatc gtatttggcg gcggcagcca tcattatttt gatgtaatcc
      781 gtcgccacct gatgtccgag gccgcgggaa ccggtatgga tcaaaacaac aacttgattc
      841 aaaaacaatc cgagtttttc ggctttttct ttgtcgaaaa tttcctcgat ttcctcgagt
      901 tccacgaaat gattaccggc gccgatcgtg ccgagctggt cgcggccgcg gtctttggcg
      961 cgtaccgaga ccattttcgg gtcggccgtc gaaagataac cgctttcttc cgcgttcaaa
     1021 gaatcttcgg gatcggcgta accgagctcg accatacgct tcactccttt aaggagaatt
     1081 tggtcgagtt ctttgtcgga gagcttcagc cttccggctc ggcccacgcc cgaaggaatc
     1141 tcttgataaa aggcttttcc cagttccgga acgcgggagc ggatttccgg aaggccgact
     1201 tccgaacaaa gaagcctcac tccgcagttg atgtcgtagc cgatgccgcc cggagaaata
     1261 atgccgtttt ccgcgtcgaa agcggccacg cctccgaccg ggaatccgta gccttcgtgg
     1321 atatcgggca tggcgaccgc gtggcccacg attcccggta gaccggccac gttcacgagc
     1381 tgatcgagcg aacggtcttc gagaatctct tcgatcattt tctcggtcgc gaaaacccgc
     1441 gccggaactt tcatgtccgg tttcgattcc cggggaatct cccagagcca atcctttatt
     1501 ttgcgaagat cggatttttc catcgctcag atgtcgaaaa cgatcgtcgt cgacaaaaat
     1561 ccgttttctt tttctcttat ttccgcttcg tgataagtga cggctttgat gttacgttcg
     1621 aaacggatgg ccggccgtgc cttgaggctg gccacgagtt ttgtcgaaga caattccttg
     1681 aaatcgacat cggtgtagaa ctcgtaattt ttctccatcc cgaaaagcac ctcgttcagg
     1741 aaatcgacaa gcaggccggt tcggtctccg gccgatattt ttatttttct cgcggtttct
     1801 ttcgagtctt ccggttctct cggaccggcg atgaaaacca ttcctttgag cgcgtctttg
     1861 aaaacttctt ccaaatcttt accttcgaca gacaaacgga tatcggctgt atgtcctaaa
     1921 attttatagg gcattgcttg atatcgatta taacaaataa ttcggaaaag atattcaatc
     1981 cacgagggcc cggcgaatat aatcgagcgt ctcgtcgtcg ggcacggctt ctccgaatct
     2041 ttccctgaag tcgttgatgt tttcgtgatc gatgcctttg agttcttccc tcaccaatcc
     2101 gtttccgtaa gcgagttcgt ccatgagatc cgtccatttt tccgcgagaa taactacctc
     2161 cggatcttgc ggctcgattt cctttgccat cgacaatccg atggcttcga tgattttcgc
     2221 gcgctccacg gcgtttttgg attcgtccat cgcttttatt ataacactaa gcctgtgata
     2281 tgatgtctcc tgaatgacaa ccattgtttt tatccacggc tacaacgctt ctccgaagat
     2341 caactggtat ccggaaatgc ggagcgagct cgacaagctc cgcatcccct accggatgcc
     2401 ggtcttgccc ggcgaagaca atccgaaaat cgaggaatgg cttaatatca tcgataaaac
     2461 catgaaatcg atcaaaggcg acgttatcct cgtcggacat agcctgggca cgagagctct
     2521 tctcctttat cttgatcgat cccgaaaacc gataaaagga gttttgctcg tctcgccttt
     2581 cagcaacgac atcggaaacg ccggtgtccg cggactcggc tattctgatt ttttcgaaca
     2641 taagatcgat ctctccgaaa tcaagaagct ttctcccaaa ttcatcgtca tgcattccaa
     2701 agacgaccaa aggattcctt tcgaacaagg aaaagaactg gccgcggaac tcggagcgga
     2761 tctcgctctc tacgaagaca agcgtcattt ttcgaggccg gaaaacgccg tggatgtatt
     2821 ggaacaactg aaaaaacttt tctgatggac ataaaatcgg cggaaaaact gttcgacgaa
     2881 aacggaaaac aaatcctcgg catcttcaaa aaaatccaga tgctcgaatt ctggctaaaa
     2941 gcgaagatcg cggacaagaa aagccggtcc gccaaggatt tcgagatcta tcaaaaatcg
     3001 ctcgaagaca agacctggaa tttcgtgatc gacgaattcc tgactcttta cccggaaaaa
     3061 gaatatctcc ggccggaact gaaacgggcc atggccgaca ggaaccggtt catgcacgcc
     3121 atctatacct gggctttgtt ttccagcaaa actcgggtgg aagaacaagg caaacgcatg
     3181 ctcgacgaaa ttgaagaaaa catcggccgg gttttgaaaa tcctgctcgc ggattgactc
     3241 tcttgccaaa ctattgaaaa aatgccgttt ttggcttatt ctttacgggt ttatggccaa
     3301 aaacgaagta attttgcggt tcgacgaagt atctttcaga tactcggctt tgaagcccat
     3361 tctcgacgag gcgagctttt ccgtgcgccg cggggccaaa atcaccctga tgggccagaa
     3421 cggcgcggga aagacgagta ttttcgggct tatcgccggc gaactcaagc ccgaatccgg
     3481 ccagatctcc gtcactccgg gagcgaccgt gggacttagc cgccaggtga tcccgcgatc
     3541 cgagctcgaa ctcacggtcc gggaattctt cgaaaaagct ttttccgcca aagtctacga
     3601 tatcgagccg cggatagaga aaatcctcaa agtggtcaat ctcgaagctc cttttgaccg
     3661 gaaagtcggt agcttttccg gcggacagca agcgagactt ttgctcgcct tcgccctgat
     3721 ccaaaatccg gacattttgc ttttggacga gccgacgaac aatctcgaca gcgaaggcat
     3781 cggccgcctc aagaaattca ttgttgaata tccgaaaacc tgcgtcgtca tttcccacga
     3841 cgccggcttc ctgaattctt tcaccgaagg cgttctctac ctcgatgttt tcacgaacaa
     3901 agtggaacaa tacgccggaa actatctcga cgtcgtcgaa gaaatttcga aaaggatcga
     3961 acgcgaaaga atgctgaacg cgaggctcga aaaagacatc gaaaaccgca aagagcaggc
     4021 caatttcttc ggccagaaag gcgggcacat gcgcgacgtg gcggccaaaa tgaaaagaaa
     4081 agtcgaagag ctcgaagaaa acaaagtgga aatgaggcgg gacgaccgcg tcatcaggag
     4141 cttcgccatt ccgacgcagg aaaacatctc cggcgacatc gtcaaaatct cttcgatctc
     4201 gagctcgaga aacggcgacc ttgtctcgaa aaaaatcccg gcgatcgttt tgaagaaaaa
     4261 agatcgtttg cttttgaccg gaccgaacgg catcggcaaa agcacttatc tcgaagccat
     4321 tgtttccgga aaagccaaag gcgtcgcgct cgcggaaaac gtgaaaatcg gctattaccg
     4381 ccaggatttc tcgacgctcg atttcgagaa gaccacctac gaatccttag ccgaagtgat
     4441 gatgaatggc ggcgaagaag aactccgttc ggccgcggcc ggatttctga tagacgccga
     4501 tactttgaag aaaaaaatca gcgatctttc cgaaggccag aaaggcctgc ttactttctg
     4561 tcgcctgatg ctccaaaagc ccgggcttct tattttggac gaaccgacga accacatcaa
     4621 tttccggcat attcccatca tcgccgacgc cgtcaaccgc tacgagggtc cggccattat
     4681 cgtgagccac atgccggact ttgtgagtaa aataaagatc acctatacga tcgatctcgg
     4741 gcaaatatag cttgacggat aaacatcaat accggatact gaatcccatg aaaaccaaaa
     4801 aagacatatt gagcgagctg atcgacgaag tcaaagagac cttgatccgc atcgaaatca
     4861 ccacccgcta cgtggaaaag aagcaagccg aaacccagaa agatcgttat ctccaagaac
     4921 tcgccaagtt acaggccaat aaggaagaaa acgaagattg gttgaaattc ttggaagaag
     4981 aactgaaaaa taccaaataa ccaatgaccg cgatgaaaat cgcggttttt tgacaattag
     5041 gcttattttg tgttatatgt aaaccataag cagttagctt tgacaactgc taattcttca
     5101 gaaggaggga acaaggttga gaaaagaaag aactcggaag gttttcgtgt tcgtcattct
     5161 cttttgtttc attcttactt cgattctcgt ctttgttccc aaagacgaga ataagccgcc
     5221 tacggaagat ccgattgcgg aggagccggt gaaacccgca gagccagtga aaccagtaga
     5281 accagacaag cctgatccga taaagccgaa gatgaagctg ctaagcttca gtgaaaaagg
     5341 agatgttgag ccgagcaaaa ttgagatcga cccaaaagtt ggagcagggg cccttctctt
     5401 t
//
LOCUS       DAOPQT010000028         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_17281_length_5228_cov_3.289967, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000028 DAOPQT010000000
VERSION     DAOPQT010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17281_length_5228_cov_3.289967"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKG74_01105"
     CDS             <1..291
                     /locus_tag="PKG74_01105"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOB89915.1"
                     /translation="KIDLDRIEKIKEAVNVAMVLHGGSGIKDDELKAAVSRGMAVVHI
                     SSELRLAWRRGLEKSFAENPGEIAPYKLLRPSTEAIADLVFRKLKLLNSVLY"
     gene            307..960
                     /locus_tag="PKG74_01110"
     CDS             307..960
                     /locus_tag="PKG74_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOB89916.1"
                     /translation="MAILLKAEKRDIFGKKTEALRQAGKVPAELYGHDTDNLHLAVLA
                     SDLRKVFGEAGGNVVVDLEIEGVKHPVLIHDTSVDPLSDEFKSVDFYKVRMDEEIVSS
                     VPLEFVGIAPAVKDLGGILVKAMDEVEVEALPANLPKALEIDVSGLIELGQSLFVKDI
                     PVRGDFKIVVDPETVIATISEPKEEVEVAPATVESVVVETEEKKAARESEQAAEAEE"
     gene            985..2397
                     /locus_tag="PKG74_01115"
     CDS             985..2397
                     /locus_tag="PKG74_01115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOB89917.1"
                     /translation="MRLKPKVLVDVKCKINAFSGLKPILNLSFRPVLRSVPDILKKTA
                     VFGVLLGVLMFNLASAPTAEFTAAAVSSVEERAALQAELDALESQIAEYEKTISAYQA
                     QGKTLTSEINKLVAKVDQLNLQIKSVNLQIRKLDDDIVVTANKISETSEEIKNTQGVI
                     AVALQKIAMSEDHSVLEILLQNPQLSDFFTNINNLMDMEDSLRLAVRQLNALKQDYVD
                     QNEQLKIAKSDAAELKQYQESQKSSLESLKTEKDTLLKETKGKESVYQQILTEKKKTA
                     AEIRSRIFEILGGGELTFEKAYELASAAEKATGVRAALILAVLDRESALGRNTGRCNY
                     KTAMHPTRDIPAFLQITSELNLDPNSTLVSCPIVADGAYGGAIGPAQFLPSTWMLYKT
                     RLAALVGVAVPNPWNNSHAFFATALYLKDAGASSNERMAAAKYYCGSNWSRYVCTNVY
                     AAAVLKKATEFQDDIDVLNS"
     gene            complement(2444..2517)
                     /locus_tag="PKG74_01120"
     tRNA            complement(2444..2517)
                     /locus_tag="PKG74_01120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2483..2485),aa:Gly,seq:tcc)
     gene            complement(2537..2719)
                     /locus_tag="PKG74_01125"
     CDS             complement(2537..2719)
                     /locus_tag="PKG74_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89918.1"
                     /translation="MNSRKALIILILAAIAILVFAAYYKWRTIRINAPETPNVPGQII
                     GPNSEPMVKGPIAPPY"
     gene            complement(3147..3605)
                     /locus_tag="PKG74_01130"
     CDS             complement(3147..3605)
                     /locus_tag="PKG74_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89919.1"
                     /translation="MQISSKLFQFNKENALIVVAGVFEADFYLASAGEINKAAGFEFD
                     KVKYENEKSIYLKAVPETGIRGGLSYDNDYQKIAIKEFTKNFGEVFNQTLKNEKIDSI
                     YILAPDKIISLIPEMLPASLKPKIKSKKEGNYLKAHPFEIIELIEELKMP"
     gene            complement(3669..3908)
                     /locus_tag="PKG74_01135"
     CDS             complement(3669..3908)
                     /locus_tag="PKG74_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89920.1"
                     /translation="MAKEQEFLRALTDLITETRGSPQRRLRDLAAEAKRIINRKGNLD
                     SIIRVLENLAKQGDIPATAFRIIDRYHDPNAIRML"
     gene            4008..4310
                     /locus_tag="PKG74_01140"
     CDS             4008..4310
                     /locus_tag="PKG74_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HOB89921.1"
                     /translation="MKGFINFIREQKVMGLAVGFILGGSVSKVVSSLVADIINPILSV
                     VLGAADDLKNAILQIGPVRILYGNFLSALIDFMVVAAVVYFGVNLIGINKLDKKKE"
     gene            complement(4353..4880)
                     /locus_tag="PKG74_01145"
     CDS             complement(4353..4880)
                     /locus_tag="PKG74_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89922.1"
                     /translation="MNQRTINILLLILLVVVVVVFTIMVIRRSMNPVAGWRTYENASQ
                     GYSIKYPPSWGLIDPIDESDLLISNYPEGDQTEADRVVLEIPGINTKSADNNLLSFIR
                     DRIAAGGDMRPSGNLVATELHNLQVLKVDHSGGDGPDGPGYYIEKSPTEYLFIAIYGT
                     DYAETVENMVSSVRF"
     gene            complement(4957..>5228)
                     /locus_tag="PKG74_01150"
     CDS             complement(4957..>5228)
                     /locus_tag="PKG74_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89923.1"
                     /translation="NICEAAMWRIRSFSNPFYKNGEEIPDQRAVSINLEARQPLFHPD
                     GRPVTMWQKDENGKRLGDAPRPLTANRCLLISDNVITLRESVKAV"
BASE COUNT         1323 a         1249 c         1350 g         1306 t
ORIGIN      
        1 aaaatcgatc tcgaccggat cgagaaaatc aaagaagcgg ttaacgtagc catggtgctt
       61 cacggcggct ccggaatcaa agacgacgag ttgaaagccg ctgtttcacg gggtatggcc
      121 gtcgtccaca tcagttccga gcttcggctc gcctggagaa gaggattgga gaagagcttt
      181 gccgaaaatc cgggagaaat cgcgccttac aagcttttgc ggccgtcaac ggaagcgatc
      241 gcggacctgg ttttccgaaa gttgaaatta ctgaactctg tgttatactg aaccgcgtaa
      301 aatatcatgg ctattttgtt gaaagcggaa aaaagagaca ttttcgggaa aaagaccgaa
      361 gctttgcgcc aggccggaaa agtgccggcc gagctttacg gccacgacac cgataacctg
      421 catttggcgg ttttggccag cgatcttcgc aaggtcttcg gggaagccgg cggaaacgtc
      481 gttgttgatt tggaaatcga aggtgtgaag cacccggtcc tgatccacga cacctccgtc
      541 gatccgttga gcgatgaatt caagagcgtg gatttctaca aagtgagaat ggacgaagaa
      601 atcgtttctt ccgtgccttt ggaattcgtg ggtatcgctc cggcggtgaa agacctcggc
      661 ggcattctgg tgaaagcgat ggacgaagtg gaagtcgaag ctttgcccgc caatctgccc
      721 aaggctctcg agatcgacgt ttcgggacta atcgagctcg gccagagcct gttcgtgaaa
      781 gatatccccg tgcggggaga tttcaagatc gtcgttgacc cggagaccgt gatcgccacg
      841 atttccgaac cgaaagaaga agtcgaagtc gctcccgcga ccgtcgagtc cgtggttgtc
      901 gaaaccgaag agaagaaagc cgctagagaa tccgagcaag cggccgaggc tgaggaataa
      961 ttcgaaatct gctaaaatta tcttatgaga cttaaaccga aagtcttggt tgacgttaag
     1021 tgtaagataa acgctttttc cggtttgaag ccgattctca atttgtcttt ccggccggtc
     1081 ttgagaagcg ttcccgatat tttgaaaaaa accgccgtct tcggcgttct tctcggagtt
     1141 ttgatgttta atctagcgtc ggccccgacc gccgaattca cggccgccgc cgtgagctcg
     1201 gtcgaggaaa gagcggcttt acaagccgag ctcgacgccc tcgaaagcca gatcgccgaa
     1261 tacgagaaaa ccatttctgc ttatcaggcc caaggaaaga ctttgacgag cgagatcaac
     1321 aagctggtcg ccaaagtcga ccagctgaat ctgcagatca agtcggtcaa tctccaaatc
     1381 aggaaattgg acgacgacat cgtcgtcacc gccaacaaga tttccgaaac ttcggaagag
     1441 attaaaaata cccaaggggt gatcgccgtc gctttacaga aaatcgccat gagcgaagac
     1501 cacagcgttc tcgagatcct gctccaaaat ccccagcttt ccgatttttt cacgaacatc
     1561 aataacctga tggacatgga agacagcctg cgtttggcgg tgaggcaact taatgctttg
     1621 aaacaggact atgtcgatca aaacgaacag ctcaagatcg ccaaaagcga cgccgccgaa
     1681 ttgaagcagt atcaggaatc ccagaaaagt tctttggaat ccctgaaaac ggaaaaagac
     1741 actcttctca aggaaaccaa gggaaaggaa agcgtctatc agcaaatact cacggaaaag
     1801 aaaaagacgg cggccgagat caggagccgg atattcgaga ttttgggcgg tggcgagctt
     1861 actttcgaga aagcttacga gctggcgtcg gccgcggaaa aagcgaccgg tgtccgggcg
     1921 gccctgatcc tcgcggttct cgatcgggaa tcggctctcg gccggaacac gggacgatgc
     1981 aattacaaaa ccgccatgca tccgacccgg gacattccgg cgtttctcca gataacgagc
     2041 gagcttaatc tcgacccgaa cagcactctg gtttcttgtc cgattgtcgc cgacggcgct
     2101 tacggcggcg ccatcgggcc ggcgcaattc ttgccgtcga cctggatgct ctacaaaacc
     2161 cggctggcgg cgctcgtcgg cgtggctgtt ccgaatcctt ggaacaacag ccatgctttc
     2221 ttcgccacgg cgctttactt gaaagacgcc ggagcgtctt caaacgagag aatggcggcg
     2281 gccaagtatt actgcggctc caattggtcg agatatgtct gcaccaatgt ttacgccgcc
     2341 gcggttttaa aaaaagccac cgaattccag gacgatattg acgttttgaa cagctgatat
     2401 tcttaaatga aacatccccg ctgtaagcgg ggattgttat ttctggagcg ggtaagggga
     2461 atcgaacccc tggcttcagc ttggaaagct gaggtattgc cactatacga tacccgcgtc
     2521 tttgtaatta cgaagtttaa taaggcggag caatcggacc tttaaccatc ggttcgctgt
     2581 tcgggccgat tatttgacct ggaacattcg gggtttccgg agcgtttata cgaatcgtcc
     2641 gccatttata ataagcggca aaaaccaaga tcgcaatggc cgccagaatc aagatgatca
     2701 aagcttttct ggagttcatg gatttagttt ataacagaaa acaaaaactg gaaaacaaaa
     2761 tggcccgcga agtgattcgc gggccgatcg gtcagtacga ctttcggaag agcaggacgt
     2821 agacggctcg gagtatggag gctgatccgg agaaccacaa tatgcgtccc caggtgccta
     2881 gcgtggcgag tgaaagcccc atgaaatata catgtgatgt tttcatgaga cacgctccca
     2941 taaccaagca acagaagccg acgtaaaaga gatcgagcgg ggtgtgaaga gaagacacga
     3001 cccaagcctt tagatatgcg gccctttcgt ggggcagcat cgggtattcg gtcgtgtaga
     3061 acctgggatt tctttgcatc ctgtccacct tcctccctca aagaacctag tgtctaataa
     3121 gtacaataaa cccgttaggc tgtcaatcaa ggcattttca gttcttcgat cagctcgatt
     3181 atttcaaacg ggtgggcttt caaataattc ccttcttttt tggacttgat tttcggtttt
     3241 aacgaagccg gaagcatttc cgggatcaga cttatgattt tatccggagc gaggatgtaa
     3301 atgctgtcga ttttttcatt tttcagggtt tggttgaaaa cctctccgaa attcttggta
     3361 aattctttta tggcgatttt ttggtaatcg ttgtcgtacg acaaccctcc tctgataccg
     3421 gtttccggaa cggccttcag ataaatactc ttttcgtttt cgtatttcac tttgtcgaat
     3481 tcgaatccgg ccgctttgtt tatttctccg gccgaagcta ggtagaagtc ggcctcaaaa
     3541 acgccggcga cgacaatcaa ggcgttttct ttattgaatt ggaaaagttt tgaggatatt
     3601 tgcatgtctt tattatacat tttattcgat aagaaaaggc cccaccatag gcggggctga
     3661 agatctgact agagcatgcg gatggcgtta ggatcatggt atctatcgat gattctgaac
     3721 gccgtcgcag ggatatcgcc ctgcttagcc aggttttcaa ggacccggat aatcgaatcg
     3781 agattgcctt tcctgttgat tatccttttc gcttcggctg cgaggtctcg aagccgtctc
     3841 tggggagaac cgcgtgtctc ggtaatcaaa tcagttaatg cccgaaggaa ttcttgttcc
     3901 ttcgccataa ctgtcatggg tttcacctct tttcgttctc gggtttccct tggggctacc
     3961 ataccttagg cctgtaattt gtcaagcgag ttttttataa tacaagcatg aaaggtttca
     4021 tcaattttat cagggagcag aaggtaatgg ggctcgcggt cgggtttatc ctcggcggca
     4081 gcgtttcaaa ggtcgtttcc tctttagtgg cggatatcat caatcctatt ctgtctgtgg
     4141 tcttgggggc ggcggacgat ctgaaaaacg ccatcctcca aatcggtccg gtccgcatac
     4201 tttacggcaa ctttttgagc gcccttatcg attttatggt agtcgccgcg gttgtctatt
     4261 tcggagtgaa tctaatcggc ataaacaaac tcgacaaaaa gaaagaataa ttctttttga
     4321 aaacagcccc gattcggggc tgttttttat tcctagaatc tgacggatga aaccatattt
     4381 tccaccgttt ccgcgtaatc cgttccgtag atcgcgatga acaaatattc ggtcggactt
     4441 ttctctatgt agtatccggg tccgtccggg ccgtcgcctc cggaatgatc gactttcagg
     4501 acttgaagat tatggagttc ggttgcaacc agattgccgc tcggacgcat gtctccgcct
     4561 gcggcgattc tgtctctgat gaaagacaaa agattgttat ccgccgattt ggtattgatc
     4621 ccgggaatct cgaggacaac ccggtcggcc tcggtctggt ctccttcggg ataattggaa
     4681 atcaggaggt ctgattcgtc gatcggatcg atgagtcccc aggaaggagg gtatttgatg
     4741 gaataacctt gagaggcatt ctcgtatgtc cgccagccgg ccacgggatt catcgacctt
     4801 cttatgacca ttatcgtgaa aacaacaacc acgacgacca aaagaattaa aagcaatata
     4861 ttgatggttc tttggttcat aagatgatta tacaattttt atcgaaatcc gaaaaaatat
     4921 aaatagaaaa gaccgatgtg cgcgtggcac atcggtctag accgccttta ccgattctcg
     4981 gagagttata acattatccg agataaggag acagcgatta gctgttagcg gacgcggagc
     5041 gtcgcccagg cgtttgccgt tctcatcctt ctgccacatc gttaccggcc ggccgtcggg
     5101 atgaaagagg ggttgacgcg cttcaagatt gatgctcacg gcgcgttggt cgggaatctc
     5161 ttctccgttt ttgtagaacg gattggagaa cgatctgatt cgccacatcg cggcctcgca
     5221 gatgttcc
//
LOCUS       DAOPQT010000029         5161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_17566_length_5161_cov_2.892479, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000029 DAOPQT010000000
VERSION     DAOPQT010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17566_length_5161_cov_2.892479"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKG74_01155"
     CDS             <1..403
                     /locus_tag="PKG74_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89924.1"
                     /translation="GALLFCKLPPGTKIVLDMEAKLREGWVIPVNKPQDEPCLTIESG
                     VYEIILIGITPTAELRTQLNETYDAASGKTSVKIESGTILGTVGPSSPSPFKNDWGVE
                     GCNLIIGIGIYSDHIDLIELSVASILEAITR"
     gene            843..2669
                     /locus_tag="PKG74_01160"
     CDS             843..2669
                     /locus_tag="PKG74_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89925.1"
                     /translation="MSGAEGLLPWGKYEIIKCIEPSIPGFCGKYYYSYHITSRLYSTA
                     DKCTNIGSSNQCLAGNQGLWAGSCTGGAGDFYKCCCNNQTNNPVSSVGYYGETNDNYP
                     PPEGVCPAGSSMKWKSGAVIDPSECQNICNPVPQENFCYHPDPDTCRYGTLEELDIDV
                     HLASTWLQLGTLRNNLTREQCSYLCNDLYVCKDCSPVSVKGKYTGYNECVDAEKLYCF
                     ERRDPVYETCSSSCTPGEETTNPGRFYYCSPLANNCVHSQQAFPSLEACEDTVNSTCY
                     LNSNCDSQCSSLTTFSWCNPDGNCSSGNYVSEESCENAVNGTCYSEREECTAVCRGSG
                     GTGCDLPADSGNDISVSLEANRQCLPNTSTGNQSRLNWNIDIDDDCAQGSPTNNRFSC
                     TSCNMPAPTTSTGSTTVSPSRTTEYGISCTRDSYRCCWEETVEEECDCVGEGENRICQ
                     TCTRTVEECDNGHGTSRQSDSVVIRVVGKPEVSFTAVPPEILYSSEEIGIRKYSNLRW
                     NSTLPNPSFASVACTLSGDGRTYDNLNPFSSLDVAPTRTTTYSLRCRNEDNIDSSCYV
                     DSDQKQTLVRVFGAGIEEQRPPQIPMIDLENLMGKIIKALGL"
     gene            complement(2688..2987)
                     /locus_tag="PKG74_01165"
     CDS             complement(2688..2987)
                     /locus_tag="PKG74_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89926.1"
                     /translation="MGSFAIIVSPGSLVRFDTELGEYLSSLLRKFINSCDNPIPLWSM
                     TVPPSLSGRQTRVCVDCLRREILITDNDRTHNEFMNSRAPSISNWKEFGMPRLIL"
     gene            3087..3689
                     /locus_tag="PKG74_01170"
     CDS             3087..3689
                     /locus_tag="PKG74_01170"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOB89927.1"
                     /translation="MKLGKTEKIKLLAALLTAAAVFMTNPRIITVEKDKNAPENINRR
                     LLAWGNTALETTRQIDTIVIHTSYNAWGRDWFDVEGIIKEYEHYDVAPHFLIDRKGQI
                     HELVPVENIADHAGRAQMPDGRKNVNDFSIGIELVNKPDTYMTRAQYDSLAGLIKDLG
                     KKYPIEHIVGHDEITTIDKTDPWNFEWEKLNYLLYFENGR"
     gene            complement(3686..>5161)
                     /locus_tag="PKG74_01175"
     CDS             complement(3686..>5161)
                     /locus_tag="PKG74_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOB89928.1"
                     /translation="DFFGKMSHWLVLSRNKNLAEFFEIVVRESGFLASALQGENVIEE
                     MDKISGLFREMKDFLEKHKSAKLKDFLEYLEMLAEHNVLLKKGNMAKLAKGVRLMTAH
                     KSKGQEFDCVYIVNAKNNHWGNKRQPNYLPLSSKIFSLLDRNPGETNSLDDERRLFYV
                     ALTRAKKEVTISYSNTASDGREQLPSQFISEIDPKMIEKIDVKDYEDEFDNHREMIFQ
                     ESSRPKVSVKDKDFVRELFSRNGLSVTALNNYLTCPWQYFYTNLFRIPKAKTKYQMYG
                     TAVHGALRDFFGALREGPVDKDFFVERFVYHLKKEPLSENGFEACLEKGRKALAGYFD
                     KYDGAWPRNSLLEFNIKGIEIAEDVKINGKIDRMDILGSGGVSVVDFKTGRPKSRKNI
                     EGETKDSKGDIKRQLVFYKLLLDNFKDAKYRMEYAEVDFVEPDEKGNYHREKFAITDE
                     ETDALKELVIKTGREILELDFWDRRCGEKDCPYCNLREMMS"
BASE COUNT         1386 a         1298 c         1154 g         1323 t
ORIGIN      
        1 aggggccctt ctcttttgta aactgcctcc gggaacaaag attgttctcg atatggaggc
       61 aaaactaaga gaaggatggg ttattcctgt taacaaacca caagatgagc catgtttgac
      121 tatcgaaagt ggtgtctatg agattatcct catcggcatc accccaacag ccgaacttcg
      181 aacgcaactt aacgaaacct acgacgctgc ttcaggaaag acctcggtca agattgaatc
      241 gggaacaatc ctcggaacag tggggcccag ttccccttct ccattcaaaa acgactgggg
      301 agttgaagga tgtaatctga taattggtat agggatttac tcagaccaca tcgacctcat
      361 cgaactcagc gttgcctcca ttctagaagc tatcacccgt tagccgcctc gacttcgaga
      421 gcggcttttc ttttccgtaa aatggtataa ttttcttaat gaaaaaaatc atctttttaa
      481 caattttcct catcggcatt ttttctttta aaacagtttt cgcccaagaa caatgtctcg
      541 aaggagattt ccctccagac agttattttc aatgtataaa tggaagactg tgtctgggtg
      601 atgaatgtat atcgtatgaa gaagttccag agacgtatgt tccattggat aatattcttg
      661 attctttaca tgaagaagcc tggtatcaaa gacagctaga attgtggaga caaacccaaa
      721 acaggccttg cgctgaacag cgaggttgtc agccatacac cacttgttct acttcataca
      781 gcgtcaatag taccaatggt tgcccgggga tttcttgtgg gcaagcaggg agaaatctac
      841 aaatgtctgg agcagaagga ttacttcctt ggggtaaata cgagatcata aaatgtatag
      901 agccatctat tccgggtttt tgtggtaaat attattattc ttaccatatt acctctcgtc
      961 tttactccac cgccgacaaa tgcaccaata taggctcttc caaccaatgt ttagcaggta
     1021 atcaaggtct ttgggccgga tcctgcaccg gaggtgctgg agatttctac aaatgctgct
     1081 gtaacaacca aaccaataat ccggtttctt cagtcggata ctatggagaa accaatgata
     1141 actaccctcc gcccgaagga gtttgtccgg ccggaagtag tatgaaatgg aagtccggag
     1201 ccgttattga tccaagtgag tgccagaata tctgcaatcc tgttccccag gaaaacttct
     1261 gctaccaccc tgaccccgac acctgcagat acggaacctt ggaagaatta gacattgatg
     1321 tacatcttgc ttccacctgg ttacagctcg gaaccttgag aaataacctc acccgggaac
     1381 aatgctctta tctctgcaac gatctctatg tctgtaagga ttgttctccg gtatcggtca
     1441 aaggaaaata taccggatac aacgaatgtg ttgacgcgga aaaactatat tgttttgaaa
     1501 gacgagatcc ggtttacgaa acctgttctt cctcttgtac tcccggagaa gaaaccacca
     1561 atcccggaag attctattac tgctctcctt tggccaataa ctgtgtccat agtcagcagg
     1621 cttttccgag tctggaggct tgcgaagaca ccgttaattc tacctgttat ttgaacagta
     1681 attgtgattc ccaatgttcc agcctaacca ctttttcctg gtgtaatccg gatggaaatt
     1741 gttcgagcgg aaattatgtc tccgaagaat cttgcgaaaa cgccgtaaac ggaacctgtt
     1801 attcggaacg ggaagagtgt acggccgtat gccggggttc cggaggaacg ggatgcgatc
     1861 tgcccgcaga ttccgggaac gacatctcgg tttctttgga agccaaccgt caatgtcttc
     1921 ccaatacttc caccgggaac caatcccgtc ttaactggaa tatcgacatc gatgacgact
     1981 gtgctcaagg ttctcccacc aacaacagat tttcctgcac aagctgcaat atgccggctc
     2041 cgaccacttc gaccggaagc accacggttt ctccttcgag aaccaccgaa tacggaattt
     2101 cttgcaccag ggactcttat agatgctgtt gggaagaaac cgtcgaagaa gaatgcgatt
     2161 gcgtcggcga aggagaaaac aggatttgcc aaacctgcac gagaacagtg gaagaatgcg
     2221 acaacggaca cggcacttcg agacaatccg acagcgtcgt catccgcgtt gtcgggaaac
     2281 cggaagtttc ctttaccgcc gtccctccgg aaatccttta ttcttccgaa gaaataggaa
     2341 tcaggaaata ttccaatctc cgatggaatt ccactcttcc caatccgagt ttcgcctcgg
     2401 tggcctgcac tttatccgga gacggaagaa cctacgacaa cctgaatccc ttcagttctc
     2461 tcgacgtcgc tcctacgcga acaaccacct actctttaag atgccggaac gaagacaata
     2521 tcgattcttc ttgttatgtc gattcggacc aaaaacagac cctggttcgg gtattcggag
     2581 ccggaatcga agaacaaagg cctccgcaga taccgatgat cgacctggaa aatctgatgg
     2641 gtaaaatcat caaggccttg ggattataaa aaagaggccc cgtctgatta taaaatcaga
     2701 cggggcattc cgaattcctt ccaattggaa attgatggag cgcggctgtt catgaactcg
     2761 ttgtgcgtcc gatcgttatc cgtgatcaat atttctctcc ttaagcaatc aacacagact
     2821 ctcgtttgcc ggccggaaag cgatggcggc accgtcatgc tccaaagcgg tatcggattg
     2881 tcgcaggagt taatgaattt ccgtaaaagc gacgatagat attctcctaa ttcggtgtcg
     2941 aatcggacga ggcttcctgg agaaacaatg atcgcgaaag aacccatttc catcgatcta
     3001 acctccttca acgtgaaaga acccgttaaa aataatcttt ggaaaagccg atgtcaatgc
     3061 ccggccgtaa aagttataat taaaacgtga aactcggaaa aacggagaaa ataaaactct
     3121 tggccgcgct tttgacggcc gcggccgtat ttatgactaa tccgaggatc atcaccgtcg
     3181 aaaaagacaa aaacgctccg gaaaacatca atcgccggct tctcgcctgg ggcaatacgg
     3241 ctttggaaac gacaaggcag atcgacacga tcgtcattca cacttcgtat aacgcctggg
     3301 gcagggattg gttcgacgtc gaaggaatca tcaaagaata cgaacactac gacgtcgctc
     3361 cgcattttct gatcgaccgg aaaggacaga tccacgaact cgtgccggta gaaaacatcg
     3421 ccgaccacgc cggacgcgcc cagatgcccg acggccggaa aaacgtcaac gatttctcga
     3481 tcggcatcga actcgtgaac aaaccggaca cttatatgac aagagcccaa tacgattcct
     3541 tggccggact aatcaaggat ctcggcaaga aatacccgat cgagcacatt gtcggccacg
     3601 acgagatcac cacgatcgac aaaaccgatc cctggaattt cgaatgggaa aagctgaatt
     3661 acctgcttta tttcgaaaac ggtcgctagg acatcatttc ccggagatta cagtaaggac
     3721 agtccttctc cccgcatctc ctgtcccaga aatccagttc cagtatttcc ctgccggttt
     3781 ttatgaccag ttctttcagg gcgtcggttt cttcatccgt gatcgcgaat ttttcccggt
     3841 gataattccc cttctcgtcc ggctcgacga aatcgacttc ggcgtattcc atccggtatt
     3901 tcgcgtcttt gaaattgtcc aacagcaatt tatagaaaac gagttggcgc ttgatgtcgc
     3961 ctttggaatc tttggtttct ccttcgatat ttttccgcga tttcggacgg ccggtcttaa
     4021 aatcgacgac gctcactccg ccggaaccca agatatccat ccggtctatt ttgccgttga
     4081 ttttgacgtc ttccgcgatc tcgatgcctt tgatgttgaa ttccaaaagc gagttcctcg
     4141 gccaagcgcc gtcatactta tcgaaatatc cggccaaggc tttccggcct ttttccaaac
     4201 aggcctcgaa accgttttcc gacagaggct cttttttcaa atggtaaacg aatctctcga
     4261 cgaagaagtc cttatccacc ggaccttcgc gcaaagcccc gaaaaaatcc cgcaaagctc
     4321 cgtgaacggc cgtgccgtac atctggtact tggtcttggc tttggggatc cggaaaagat
     4381 tggtgtaaaa atactgccaa gggcaagtca aataattgtt caaggccgtg acggaaagac
     4441 cgttcctcga aaacaattcc ctaacaaaat ccttgtcttt gacgctcact ttgggccgcg
     4501 atgattcctg gaaaatcatt tcgcggtggt tgtcgaattc gtcttcgtaa tctttaacgt
     4561 cgattttctc gatcattttc ggatcgatct ccgaaatgaa ttgcgaagga agctgttccc
     4621 ggccatccga agcggtattc gagtaagaga tcgtcacctc ttttttcgcc cgggtcaaag
     4681 ccacgtaaaa gagccggcgt tcgtcgtcaa ggctgttcgt ttctcccgga ttccgatcta
     4741 aaagagaaaa gattttcgaa gacaaaggaa gataattcgg ctgacgcttg tttccccaat
     4801 ggttgttttt ggcgttcacg atgtaaacgc agtcaaattc ctggcctttt gatttgtggg
     4861 cggtcatcag cctcactcct ttggcgagtt tcgccatgtt ccccttcttc aaaagcacgt
     4921 tgtgctcggc gagcatttcg aggtattcga gaaaatcctt cagtttggcg gatttgtgct
     4981 tttccaaaaa gtccttcatt tcccggaaaa gaccggagat cttgtccatt tcctcgatga
     5041 cgttttctcc ttggagcgcc gaggccaaga atcccgattc ccggacgacg atctcgaaaa
     5101 actcggccag gtttttattg cggctcaaaa cgagccagtg ggacattttc ccgaaaaaat
     5161 c
//
LOCUS       DAOPQT010000030         5138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_17663_length_5138_cov_3.226835, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000030 DAOPQT010000000
VERSION     DAOPQT010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17663_length_5138_cov_3.226835"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKG74_01180"
     CDS             <1..294
                     /locus_tag="PKG74_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOB89929.1"
                     /translation="SAAYYARYVAKNIVAAGLSRNCVVAVAYAIGRAEPLMLEAIGEN
                     GKDLTEVLKRNFDFRPRAIIERLGLRKPIYRETSVYGHFGKENLPWERMVDMI"
     gene            396..656
                     /locus_tag="PKG74_01185"
     CDS             396..656
                     /locus_tag="PKG74_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017696756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HOB89930.1"
                     /translation="MAVVKKKIWPRYFELVNSGKKKFELRLADFMIKEGDTLVLEEWD
                     NEKKRLTGRKITRKVSYILKFDLDDFGQKKEILKDGLYVIQF"
     gene            703..1065
                     /locus_tag="PKG74_01190"
     CDS             703..1065
                     /locus_tag="PKG74_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOB89931.1"
                     /translation="MQKNIIWIIVVIVAVIGIYYAYGFMKKGSPTETPSGSGNASEVL
                     ISNFSFAPKELTIKRGTTVKWTNRDNVDHPIVSDAGVFQSGAIVPGGTFEFAFDSIGE
                     FSYHCSAHPWMTGKIIVE"
     gene            1084..2283
                     /locus_tag="PKG74_01195"
     CDS             1084..2283
                     /locus_tag="PKG74_01195"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOB89932.1"
                     /translation="MQKNITAVAIIVLAIVLVLMVGSYYRSRLSLENPSGNVSAAPEG
                     IDLPEGFTMSVFAENLTAPRVLAFDPNGVLVASITSTGEIVALPSDNPDNPVAEKTVI
                     LASGLNRPHGLAFYCKDSCKLYVAETDNVSVFDYDADNLKLSNKKKIIDLPEGGQHFT
                     RTLLILASDDGDRLLVALGSDCNVCVESDWRRASILSADMDGKDLKTFASGLRNSVFM
                     ALEPQSGKVFATENGRDLLGDDIPPDEINIIEEGKNYGWPICYGNNIHDGDFDKNVYI
                     ADPCRDKTASHIDIQAHSAPLGLAFVPDNWPSEYRNDLFVSLHGSWNRSVPTGYKVTR
                     FSLDAGGLYSGSGPEQVDFITGWLDSDGKISGRPTGIAFDSYGNMFVADDRAGAIYKI
                     VPPVLGS"
     gene            2350..2541
                     /locus_tag="PKG74_01200"
     CDS             2350..2541
                     /locus_tag="PKG74_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOB89933.1"
                     /translation="MKTLSWIAWILVVIGAINWGLYGLFGMNLVMAIFGSMPMVEKVV
                     YVLVGISGIWMIFDKMQKS"
     gene            2606..2827
                     /locus_tag="PKG74_01205"
     CDS             2606..2827
                     /locus_tag="PKG74_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOB89934.1"
                     /translation="MIVKVKVLPNSSKSGIEHRSDVLVIKTRSKAEAGKANAEALELL
                     ARHFKVLVSKLKILKGSRSKNKIIKIGEG"
     gene            2925..3869
                     /locus_tag="PKG74_01210"
     CDS             2925..3869
                     /locus_tag="PKG74_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89935.1"
                     /translation="MARGLAATVAADVAKVRKAATVLIEEDGGQFGYQVTNEANMLSP
                     QSGLITVIGPTPDFDKAVLAEVGIGHTRPYGEMEHIRLENGSKVSVERRQTRFQMSVD
                     QYLEVAALIFIGMGQVAYERTKKWRFTTWTNEKMPHTRSSKSGRKTVRYNKDYEMIKG
                     IRFSVSIGFAPLIADLFWAEKRSFRDYYVGLGKMIDMRLAPYRRNGYHRLFNTLLGAK
                     DLIDIVESRDFLKPAYRRFEALEFLPKGDQHPLYYEVAKDVFGHDFFKVPEEIQIMKS
                     SLPDPEERIPREIYCRGINTLAEAKEKGHMVFEVSWRS"
     gene            3919..4497
                     /locus_tag="PKG74_01215"
     CDS             3919..4497
                     /locus_tag="PKG74_01215"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOB89936.1"
                     /translation="MEVHKTLNDKIAVAVAAGRIAVIPTDTIYGIVGSALRPETVEKI
                     YRLRKRNRTKPMIILIGELKQLSFFGIKIDLKTKKKLDRFWPGKTSIILPCRSPKFRY
                     LHRGKHTLAFRLPALKSLSKFLLKTGPLVAPSANIEGMPPARNISEAKRYFGTKVDLY
                     VDQGPRQGEPSALIDLSGKTIKVLRPGSMRTS"
     gene            4494..>5138
                     /gene="truB"
                     /locus_tag="PKG74_01220"
     CDS             4494..>5138
                     /gene="truB"
                     /locus_tag="PKG74_01220"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOB89937.1"
                     /translation="MIEGIFPIYKEKGLTSNRTIQELKKIVGKNKIGHAGTLDPLAEG
                     VLVVGIGRKFTKELHKEEAKEKEYLAVIHLGATSSTDDEEGTKTLREIKQRPSILEIR
                     RVIGKFVGEIEQKPPVFSAIKIKGREAYKLARRGEYPEMKARKVEIKKIKIVSYAWPD
                     LKIRVITAPGVYIRSLARDLGEALGTGGYLRSLIRTRVGNFSAEKSFTLSEFKEK"
BASE COUNT         1337 a         1219 c         1347 g         1235 t
ORIGIN      
        1 tcggcggcct actacgcgcg atacgttgcc aagaacatag tcgccgccgg tctttcccga
       61 aactgcgtcg ttgccgtggc ttacgccatc ggccgggccg aacccttgat gctcgaagcg
      121 atcggcgaga acggtaaaga tttgaccgaa gttttgaaac gtaatttcga tttccgtccg
      181 agggcgatca tcgagaggct cggcctcaga aagccgatct accgcgagac atcggtctac
      241 ggccatttcg gaaaagaaaa tctgccttgg gagcgaatgg tcgatatgat ataattgttc
      301 catagagcct ccttgatttt tccgcgatga ctttctcgag cggcgattgg tccaccggct
      361 cgctttatca aaggtcgcaa taaaaaaatc aataaatggc agttgtcaaa aagaaaatct
      421 ggccgcggta tttcgagttg gttaattccg gcaagaaaaa attcgagctc cgcttggccg
      481 atttcatgat taaagaaggc gacaccctgg tgctcgaaga gtgggataac gagaaaaaga
      541 gattgaccgg acggaaaatc accaggaagg tgagttatat cctgaagttc gatttggacg
      601 atttcgggca gaaaaaagag attctgaaag acggattgta cgtgatccag ttttgacgcc
      661 tgctttgccg caagcaagag ttcgctttcg gctttgtgat ccatgcagaa aaatatcatt
      721 tggataatcg tcgtcattgt cgcggttatc ggaatctatt acgcttacgg attcatgaaa
      781 aaaggatcgc cgacggaaac gccttcgggt tccggaaacg cttccgaggt actcatatct
      841 aatttctctt tcgcaccgaa agagcttacg atcaaaagag gaacgaccgt caaatggacg
      901 aaccgagaca atgtcgatca tccgatcgta agcgacgccg gcgtgttcca gagcggcgcc
      961 atcgtgccgg gaggaacatt cgaattcgcc ttcgactcga tcggagagtt ttcttatcat
     1021 tgctccgctc atccgtggat gaccgggaaa atcatcgtcg aataatcagc ctttaaaaaa
     1081 aagatgcaaa aaaacatcac cgcggtcgcc atcatcgttc tcgcgatcgt cctggtgctc
     1141 atggtcggct cgtattatcg gagcaggctt agcctcgaga atccgtcagg gaatgtttcg
     1201 gcggctccgg aaggaattga tctaccggag ggatttacta tgtcggtttt cgccgagaat
     1261 ctcaccgctc cgcgagttct cgctttcgac ccgaacggcg ttcttgtggc gagcatcact
     1321 tcgaccggag aaatagtcgc tttgccctcc gacaatccgg acaatcccgt ggcggaaaaa
     1381 accgttatct tggcttcggg cctgaacagg cctcatggtt tggcgtttta ttgtaaagac
     1441 tcctgcaagc tttatgtggc cgagacagac aatgtttccg ttttcgatta cgacgcggac
     1501 aatctcaagc tgtccaacaa gaaaaagatc atcgatctgc cggaaggagg ccagcatttc
     1561 acgaggactt tgcttattct agcttcggac gacggagaca ggcttcttgt cgctttgggg
     1621 tcggattgca acgtttgcgt cgaatccgac tggcgccgag cgagcatcct gtcggccgac
     1681 atggacggga aagatttgaa gactttcgct tccgggcttc gcaattccgt ttttatggcc
     1741 ttggagccgc aaagcggcaa ggtttttgct accgagaacg gacgcgatct tttgggagac
     1801 gatatcccgc ccgacgagat caacatcatc gaagaaggga agaattacgg ctggccgatc
     1861 tgctacggaa ataatatcca tgacggagat ttcgacaaaa acgtctatat cgccgatcct
     1921 tgccgcgaca aaacagcgag ccatatcgat atccaggcgc attccgctcc tttgggactc
     1981 gcttttgttc cggacaattg gccgagcgag tatcggaacg atttattcgt ctctctccac
     2041 ggatcgtgga acagatcggt tccgaccggc tacaaagtca cgagattttc tttggatgcc
     2101 ggcggccttt attcgggaag cgggccggaa caagtcgatt ttatcaccgg ctggctcgat
     2161 tcagacggaa aaatttctgg aagacccacg ggaatcgcct tcgattctta cggcaatatg
     2221 tttgtcgccg acgaccgggc cggagcgatt tacaagatcg ttcctccggt tttgggtagt
     2281 tgatccgtcg ttcggaacct ctttatgcta taatttgatc aataaaggtc gcttaataaa
     2341 ctttatgtca tgaaaacttt aagttggatt gcctggattc tggtggtcat tggcgctatc
     2401 aactggggat tgtatggcct ctttgggatg aatctggtaa tggcgatttt cggctccatg
     2461 ccgatggtgg aaaaggttgt ctatgtcttg gtcggaatct ccggtatctg gatgattttc
     2521 gacaagatgc aaaagtcgta attttgcgac ttcttcaaat ccctcgcttc ggcgggggat
     2581 tttttgacgt tataatgaga caaagatgat cgttaaagta aaagtcctgc caaactcttc
     2641 caaatccgga atcgaacacc gatccgacgt tttggtcatc aagacgagat ccaaggccga
     2701 agcgggtaaa gccaatgccg aggccttgga gcttctcgcc cggcatttca aggttctcgt
     2761 atccaaactg aaaatcctca aaggatcgcg gtccaaaaat aagattatca agattggaga
     2821 aggttaagtt gtcgaacccg gccgcggatt ttgactccgt gaccttttga ctctataaat
     2881 aactgtcgcc aggtgtttga aaaccgaaga aaggaggcgg caatatggcg cggggtcttg
     2941 cggccacggt tgcggccgat gtggccaaag ttagaaaagc ggccactgtc ttgatcgaag
     3001 aggatggggg gcaatttggc tatcaggtaa ccaacgaggc gaacatgctt tctccccagt
     3061 cgggtttgat cacagttatt ggcccgacac cggatttcga caaggcggtt ctcgcggaag
     3121 tgggaatcgg ccacacaagg ccgtatggag aaatggagca tattcgtctt gaaaacggct
     3181 ccaaggtcag tgtcgagcgt cggcagactc gtttccaaat gagtgtcgat cagtatttgg
     3241 aggtagccgc tcttatcttt atcgggatgg gccaagtggc gtatgagcga actaaaaaat
     3301 ggcggttcac cacttggacc aacgagaaga tgcctcatac ccgttcgagt aaatcgggac
     3361 gcaaaacggt ccggtacaat aaagattatg aaatgatcaa aggtatccgt ttcagcgtct
     3421 cgatcgggtt tgcaccgctc atcgctgatc tgttctgggc cgagaaaaga tcattccggg
     3481 attactatgt tggtctcggc aagatgatcg atatgaggtt ggcaccatat cggcggaacg
     3541 gataccaccg tctcttcaat acgctactcg gagcaaagga tttgatcgat atcgtcgagt
     3601 cccgcgactt ccttaagccg gcttatcgtc gcttcgaagc gctcgaattc cttccgaagg
     3661 gcgaccagca tcctctttac tatgaagtcg ccaaagatgt ctttggtcat gatttcttca
     3721 aagtcccgga ggaaatccag atcatgaaat cctctcttcc ggatcccgaa gagaggattc
     3781 ctcgagagat ctattgccgc ggcataaata ctcttgccga ggccaaagaa aagggacata
     3841 tggtgtttga ggtttcatgg cgttcttgag cgcccgacaa cattgttgcc gggcgcttat
     3901 tgtatctata atcattccat ggaagttcat aagaccttga acgataaaat cgccgtggcc
     3961 gtggcggccg gccggatcgc cgtcattccg acggacacga tttatggcat cgtcggctcg
     4021 gcgcttcgac cggagaccgt ggaaaaaatc tatcgtctgc ggaagagaaa caggacaaag
     4081 ccgatgatca ttttgatcgg cgaattgaaa caactttcgt ttttcggaat aaaaattgat
     4141 ttgaaaacaa agaaaaaact cgatcgtttc tggccgggaa aaacgagtat tattcttcct
     4201 tgccgttctc cgaagttccg gtatcttcac cggggcaaac atactttggc cttccgtttg
     4261 cccgctttaa aatccctgtc gaaatttttg cttaaaaccg gaccactggt cgctccgagc
     4321 gcgaacatcg aaggaatgcc tccggcccga aacatcagcg aagccaaaag atattttgga
     4381 accaaagtcg atctttacgt cgatcaaggc ccgaggcagg gcgagccttc ggctttgata
     4441 gacttgtccg gtaaaaccat taaagttcta agacccgggt caatgagaac atcatgatcg
     4501 aaggcatttt cccgatttac aaagaaaaag gtctgacttc gaacaggacg atccaggaac
     4561 tgaaaaagat cgtcggaaaa aacaaaatcg gccacgccgg gactctggat ccgctggccg
     4621 agggtgtgtt ggtggtcggg atcggccgta aatttaccaa agaactccac aaggaagaag
     4681 ccaaagaaaa agaatacttg gctgttatcc atctcggtgc gacgagctcg accgatgacg
     4741 aagaagggac aaagaccttg cgcgagataa agcaaaggcc gtcgattctc gagatccgcc
     4801 gggtgatcgg gaaattcgtc ggcgagatcg aacaaaagcc gccggtattc tcggcgatca
     4861 aaatcaaagg cagagaagcc tacaaattgg cgagacgggg agaatatccg gaaatgaaag
     4921 cccgcaaagt ggaaatcaag aaaataaaaa tcgtttctta cgcttggccg gacttgaaaa
     4981 tccgggtgat aacggctccc ggcgtctata tccgatcgct cgcgagggac ttgggcgagg
     5041 cgctcggcac gggcggctat cttcgatccc ttattcggac aagagtcgga aatttttccg
     5101 ccgaaaaatc gttcaccctt tccgaattca aagagaag
//
LOCUS       DAOPQT010000031         4829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_19194_length_4829_cov_3.171973, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000031 DAOPQT010000000
VERSION     DAOPQT010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19194_length_4829_cov_3.171973"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..562)
                     /gene="def"
                     /locus_tag="PKG74_01225"
     CDS             complement(104..562)
                     /gene="def"
                     /locus_tag="PKG74_01225"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOB89938.1"
                     /translation="MMKIVTVEDKKSEAKILHGRVPDFDFKKADKKKIAEVAKKMRKL
                     MQENKGVGLSANQIGLDWRMFVAQNEGKFYVIFNPEITSVSKQMDECEEGCLSVPEME
                     KSIKRPYQVTLVGQDKNGKKLKIKAWGLLARIFQHETDHLNGTLIADHKD"
     gene            complement(534..851)
                     /locus_tag="PKG74_01230"
     CDS             complement(534..851)
                     /locus_tag="PKG74_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89939.1"
                     /translation="MESMKTGRKKEKKRDFADYFRKIKERAKESRVYTRHQLVGLEMA
                     ELLDDRAHKSLYIKLAKEFNEDRLLSLAKSIAEKKNIKNKGAYFMAIWQKEKDDEDSH
                     RRR"
     gene            867..1430
                     /gene="thpR"
                     /locus_tag="PKG74_01235"
     CDS             867..1430
                     /gene="thpR"
                     /locus_tag="PKG74_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOB89940.1"
                     /translation="MDKRVFAGINLPLETKAKIEAALFEAEPLWKGIISPRILDRENW
                     HITMGFFGKKSEAEIELIKEAIEETASAFPLPEIKLKRIVFGPTKAREARMIWLEVES
                     KDLKNVKESFDRNLEKRGVILENKKFSPHITLARFEPIDFDRLPPLKKEVDLDFRAKS
                     LDLMESVLSSKGSTYTLISEIDFGGVK"
     gene            1441..2685
                     /locus_tag="PKG74_01240"
     CDS             1441..2685
                     /locus_tag="PKG74_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOB89941.1"
                     /translation="MMNQLPKKRILIVVVLALLLAVSTAGSFYVGYRKGLEETKIIEI
                     KGVADINPNEEVSADFGVFWQAWDKIKSKYVKAKDIKDQDLVYGAISGMLESLGDPYT
                     VFMNPEEAQKFTEDVNGNFGGIGAELENQGDYVVVVAPLKGTPAERAGIKPGDIILKA
                     GDTELTDVDVLEAVKVIRGEPGTKIILSVMREGFTAPKDFEITREIIEVPTLEWSWLD
                     DKIVHIELYGFNQNAPVAFYRAILETVPQDTKGIVLDLRNNPGGYLDVAVNIAGWFLK
                     PGETVVSEKFANGDLDVLRASGNGALQNMPTIVLVNSGSASASEILAGALRDNRNVKI
                     VGEKTFGKGSVQTIEDLKDGSVLKVTIASWVTPKGLILNGNGIEPDYEVKITDEDAEN
                     DRDPQLEKALEILESQITATGN"
     gene            2699..3133
                     /gene="rplI"
                     /locus_tag="PKG74_01245"
     CDS             2699..3133
                     /gene="rplI"
                     /locus_tag="PKG74_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOB89942.1"
                     /translation="MKVILLEDIKNVGRRHEVKNVSDGYARNMLFPRKMAVLATPEEM
                     RKIEKEKQTAETEEKESMAKLKSVTEGEPIKFYLKTGDKGEIYTSIKSEDIEQELRRR
                     GLGEVKVKLPKPIKNLGLFEVEINLRKGVAGKVKISAEPSPK"
     gene            complement(3102..3383)
                     /gene="rpmA"
                     /locus_tag="PKG74_01250"
     CDS             complement(3102..3383)
                     /gene="rpmA"
                     /locus_tag="PKG74_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009629204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOB89943.1"
                     /translation="MAHTKALGSTKLGRDSEAKRLGVKKNHGEPVKPGQILLRQRGTR
                     YIPGDNVLRAGDDTLYSKIEGVVQYSKGKKTRFNSKIRYATWVKVLPKS"
     gene            3401..3550
                     /locus_tag="PKG74_01255"
     CDS             3401..3550
                     /locus_tag="PKG74_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89944.1"
                     /translation="MKFMDLSTGKTRIFLAVVGVLILSLAFGLGYFSGQSFCRNPIVI
                     ETCAD"
     gene            3538..4242
                     /locus_tag="PKG74_01260"
     CDS             3538..4242
                     /locus_tag="PKG74_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89945.1"
                     /translation="MRRLISVSTGCFYKLGVSKEEIFGRLKALDIDGVEVLFNKPQDV
                     FDFEISEDNSDFLRSLSFVTIHAPDNDLENPAAIEVLNRLDHIYRLIYAKNIVFHVRK
                     DDDLSIFFNYGFKASMENDDWRKEKETNTVPSIERLLRIYPRLGFVLDVGHADSVDPA
                     DAEKYADRFKDRITEIHLSRVDKEKREHDFMNRHRESLNKKVFEATRVPIIIEGALQN
                     ENVSALQKEIELARKL"
     gene            complement(4239..>4829)
                     /locus_tag="PKG74_01265"
     CDS             complement(4239..>4829)
                     /locus_tag="PKG74_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89946.1"
                     /translation="SADEGTLVHEAIEDILLGHSPSSDPRVKPAVDAFLKFNESRRIV
                     ANPDWVEHKVINRDDRYAGTVDALATIDGKFGVLDIKTSQAIYRDYNLQTSAYLVPLQ
                     KEMRDLSTRWILRIDQNQKCAKCGATRRVKGGREKIKGLNGRNPNLCEHEWSEIKGEV
                     ELQEFPYFIDDYQAFLGAKKLWEWENEYWLKQIGYL"
BASE COUNT         1239 a         1149 c         1208 g         1233 t
ORIGIN      
        1 tctcgctagc ctcctctctt caagattgtc tgcagttgtg aaattaactg cttatgattt
       61 tttcatagca taaacaggga agattgtcaa aatatccggt tttctaatct ttgtgatcgg
      121 cgatcaatgt gccgttcaga tgatcggttt cgtgctggaa tattcttgcg agtaatcccc
      181 aggctttgat tttcagtttt ttgccgtttt tatcctgtcc gacaagcgtt acttggtaag
      241 gtcgtttgat gcttttttcc atttcgggaa cgctcaagca tccttcttcg cattcgtcca
      301 tttgcttcga aacactcgta atttccggat tgaaaataac gtaaaacttg ccttcgttct
      361 gggcgacgaa catccgccag tccaaaccga tctgattcgc cgaaagcccg acgcctttgt
      421 tctcctgcat caacttccgc atttttttgg caacttcggc aatcttcttt ttgtcggctt
      481 tcttgaaatc gaaatccggc acccgtccgt gaaggatctt ggcttcggat tttttatctt
      541 ctacggtgac tatcttcatc atctttttct ttttgccaga tggccatgaa ataggcccct
      601 ttgttcttga tgttcttttt ctcggcgatc gatttggcga ggctcaaaag cctgtcttcg
      661 ttaaactcct tcgccaattt gatatataag gatttgtggg ctctatcgtc aaggagctcg
      721 gccatttcga ggccgaccaa ctggtgtcgg gtgtaaaccc gggattcttt ggccctttcc
      781 tttattttcc ggaaataatc cgcgaaatcc cgcttttttt cctttttccg gccggttttc
      841 atcgattcca ttatataata ctctcgatgg ataaaagagt cttcgcggga atcaatcttc
      901 ctttggaaac aaaagcaaaa atcgaagcgg ctttgttcga agccgaacct ttatggaagg
      961 ggattataag cccgagaatc ctggatcggg aaaactggca tatcactatg gggtttttcg
     1021 ggaagaaaag cgaggcggag atcgagctta tcaaagaagc gatcgaagaa accgcctcgg
     1081 cttttccttt gccggaaata aaattaaaaa gaatcgtctt tggtccgacc aaggctcgtg
     1141 aagcgagaat gatctggctc gaagtcgaat ctaaagatct caagaatgtc aaagaatctt
     1201 tcgatcgaaa tctcgaaaaa cgcggagtga ttttggaaaa caaaaagttt tctccgcaca
     1261 tcactttggc gagattcgag ccgatagatt tcgaccgttt accacctctt aaaaaagaag
     1321 tcgatctcga tttccgggca aagtctcttg atcttatgga gtccgtatta tcgagcaaag
     1381 gatcgactta taccttgatt tcggaaatag actttggagg ggtaaaatag taaaatccct
     1441 atgatgaacc agctgccgaa aaagagaatc cttattgtcg ttgttctcgc ccttttgctt
     1501 gcggtctcga ccgccggctc tttttatgtc ggctaccgta aaggcctcga ggagacgaaa
     1561 atcatcgaaa tcaaaggcgt ggccgacatc aatccgaacg aggaggtttc ggccgatttc
     1621 ggggtatttt ggcaagcctg ggacaagata aaatccaaat acgtcaaagc caaagacatc
     1681 aaggaccagg atctggttta cggagcgatc agcggcatgc tcgaaagtct cggcgatcct
     1741 tataccgttt tcatgaatcc ggaagaagcc cagaaattca ccgaggacgt taacggcaat
     1801 ttcggcggca tcggagccga actggaaaac cagggagatt acgtcgtggt cgtggcgccg
     1861 ctcaaaggaa ctccggccga acgggccgga ataaaacccg gcgacatcat tctgaaagcc
     1921 ggagacacgg aattgaccga tgtcgatgtt ttggaggcgg taaaagtgat ccggggagag
     1981 ccgggaacaa aaatcattct ttccgtcatg cgcgaaggat tcaccgcacc caaggatttc
     2041 gagatcacga gggaaatcat cgaggttccg accctcgagt ggtcctggct cgatgataag
     2101 atcgtccaca tcgaacttta cggattcaat cagaacgcac ccgtcgcttt ttaccgagcc
     2161 atcctcgaga ccgtgcccca ggacaccaag ggaatcgtcc tcgatttgag gaacaatccc
     2221 ggcggatacc ttgatgtcgc cgtaaacatc gccggctggt tccttaagcc cggcgaaacc
     2281 gtcgtctccg agaaattcgc gaacggcgat ctcgacgtgc tgcgggcttc gggaaacgga
     2341 gctctccaaa acatgccgac gatcgttctc gtgaacagcg gttcggcttc ggcttcggag
     2401 atcctggccg gcgcgctccg cgacaaccgc aacgttaaaa tcgtcggcga aaagactttc
     2461 ggcaaaggat cggtgcagac gatcgaggac ctgaaggacg ggtcggtcct gaaagtgacg
     2521 atcgcgagct gggtgacgcc caaaggattg attttgaacg gcaacggcat cgaacccgat
     2581 tacgaagtca aaatcaccga cgaagacgcg gaaaacgacc gcgacccgca gctcgaaaaa
     2641 gcgctcgaaa tcctggaatc gcaaatcacg gcgacaggaa attaattttc aaaaacaaat
     2701 gaaagtcatt cttctcgaag acatcaagaa tgtcggccgc aggcacgaag tgaagaacgt
     2761 ttctgacggc tatgcccgga acatgctttt tccgaggaaa atggcggttt tggccacgcc
     2821 cgaggaaatg agaaaaatag aaaaagaaaa acaaacggcc gagaccgagg aaaaggaatc
     2881 gatggctaag ctgaaatccg tcaccgaagg cgagccgatc aagttctacc tcaaaaccgg
     2941 tgacaaaggg gaaatctaca cctcgatcaa atcagaggac atcgaacaag aactccggag
     3001 aaggggactc ggagaggtca aagtaaagct tccgaaaccg ataaaaaatc tcggcctttt
     3061 cgaggtcgag atcaatttgc ggaaaggggt cgccggaaaa gttaagattt cggcagaacc
     3121 ttcacccaag tagcgtatct tattttggaa ttgaaccgcg ttttcttgcc cttggaatac
     3181 tggaccacgc cttcgatttt ggaatagaga gtatcgtctc cggctctcaa gacattgtct
     3241 ccgggaatat agcgggttcc tctttggcga agcaaaatct gaccgggttt gaccggttct
     3301 ccgtggttct tctttactcc caatctttta gcctcggaat cccgtcctaa ctttgtggat
     3361 ccgagggctt ttgtatgagc cataacaaaa taatgataat atgaaattca tggacctgtc
     3421 aaccggaaaa acaaggattt tcctcgctgt cgtcggggtt ttgatcctga gtttggcttt
     3481 cgggttgggg tatttttcgg gtcagagttt ttgccggaat ccgatcgtta tcgaaacatg
     3541 cgccgattga tttccgtttc cacgggttgt ttctacaaac tcggcgtttc caaagaagaa
     3601 atcttcggac gcctcaaggc tttggacatc gacggcgtcg aagtactttt caataagccg
     3661 caggacgttt tcgattttga aatatccgaa gataattcgg atttccttag aagcttgtct
     3721 tttgtcacga tccatgcgcc ggacaacgac ttggaaaacc cggcggcgat cgaggtttta
     3781 aaccggttgg atcatattta tcggctgatc tacgccaaaa acatcgtttt tcacgtacgc
     3841 aaagacgacg acttgagtat tttcttcaat tacggcttta aagcatccat ggaaaatgac
     3901 gattggagaa aagagaaaga gacgaatacc gtccccagta tcgagcgcct gctccgcatt
     3961 tatccgcgcc tgggatttgt tcttgatgtc ggacacgccg attcggtcga tccggccgac
     4021 gccgaaaaat acgccgatcg ttttaaagac aggatcacgg agatccatct atcccgtgtc
     4081 gataaagaaa aacgcgaaca cgattttatg aatcgacacc gagaatcgtt gaataaaaaa
     4141 gtattcgagg cgacgcgagt gccgataatc atcgaaggag cgcttcagaa cgaaaacgtg
     4201 tcagcgcttc aaaaggaaat cgaactggcc cggaaacttt agaggtatcc gatttgcttc
     4261 agccagtatt cgttctccca ttcccagagt tttttggctc cgaggaaggc ttgatagtcg
     4321 tcgatgaagt aggggaattc ctggagttcg acttcgcctt tgatctcgga ccattcgtgc
     4381 tcgcagagat tcggattccg gccgttcagg ccttttattt tttcccggcc gcctttgacg
     4441 cgcctggtag ctccgcactt cgcgcatttc tggttttggt cgatcctcaa gatccacctt
     4501 gtcgaaaggt cgcgcatttc cttttggagc ggaacgagat aggccgaggt ttggagattg
     4561 tagtcccggt aaatggcctg ggaagttttg atgtcgagaa ctccgaattt gccgtcgatc
     4621 gtcgccaaag cgtcgacggt tccggcatag cggtcgtcgc gattgatgac tttgtgttcg
     4681 acccagtcgg ggttggccac gatgcgtcgg gattcgttga atttcaagaa agcatcgacc
     4741 gcgggtttga cccgaggatc cgaagaaggg gagtgcccga gcaggatgtc ttcgatcgct
     4801 tcgtggacca aggtgccttc gtcggccga
//
LOCUS       DAOPQT010000032         4738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_19653_length_4738_cov_3.408926, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000032 DAOPQT010000000
VERSION     DAOPQT010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19653_length_4738_cov_3.408926"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..564
                     /locus_tag="PKG74_01270"
     CDS             151..564
                     /locus_tag="PKG74_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89947.1"
                     /translation="MFDLLKIFFIKKAIANGGNLPTPAGEGSLPSSVGGGSLSSGESA
                     GGGNLFDPGKFLPGGLPTDLGALIETIFRWVLWAATLVFLYQAFRAAWDLITKKEAET
                     ALRDAKKRLWNALIGFMILLFAQSIPGIIRDILTG"
     gene            638..1006
                     /locus_tag="PKG74_01275"
     CDS             638..1006
                     /locus_tag="PKG74_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89948.1"
                     /translation="MKKINYSTVVAAIAANTTIAFALVLPPIIPGVGILDPKAWSWAE
                     IASWPNIILNWFFWLITIWTVWMILRTALDFVNSAGDPKKMGELSTRGRNIAIGVILA
                     ILARSIPAIISTIISSSGQN"
     gene            complement(1064..1612)
                     /locus_tag="PKG74_01280"
     CDS             complement(1064..1612)
                     /locus_tag="PKG74_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89949.1"
                     /translation="MITITIIMAIVGPKGWTSAILVAGLIGTLTHFILRLTNVNKRWA
                     VVFAMVIAMMAVAKFSPVKLADRVTAPITPPPYSEVPDPGYKIRESKEMVEITIVLQG
                     DKGVEGPSFNTLAAKWPGWRFDFYPETLDVEVQFPGGKRVSWRGEWGIKTGTPTFWGP
                     AGEEITIILTPPSSSPPAVITL"
     gene            1828..1911
                     /locus_tag="PKG74_01285"
     tRNA            1828..1911
                     /locus_tag="PKG74_01285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1862..1864,aa:Leu,seq:tag)
     gene            2034..2279
                     /locus_tag="PKG74_01290"
     CDS             2034..2279
                     /locus_tag="PKG74_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89950.1"
                     /translation="MKKEGDVIIHAKEHESSFGLGDLLLAAVTGGVSLFLPDPKYEAE
                     ALSISTNTSAKGYGDTKAEAEMNAGKELAKELEKRRP"
     gene            complement(2352..3239)
                     /locus_tag="PKG74_01295"
     CDS             complement(2352..3239)
                     /locus_tag="PKG74_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOB89951.1"
                     /translation="MIEKYLKDYLDYLQIEKNRSPRTKDVYERALKKFFEFSKIKKPA
                     DITNDKIRSYRVYLAEKTDLKKNTQSYRIIAIRNFLRYLVKMDVKVVSPDRIELPQIP
                     KRQIDIIEYEDLERILEAPAGSTLKALRDRAILELLFSTGLRVSELCSLDRYLNWKRG
                     EITVRGKGEKLRVVFISDRAKKAIKNYLDKRADAEEALFVSYSNGSNPRVIGRITPRS
                     VERLVYFYSRKAGVSRKVSPHTLRHLFATDLLMNGADLRSVQEMLGHASIQTTQIYTH
                     LTNKTLKEIHDAFHGRRRE"
     gene            complement(3285..3824)
                     /locus_tag="PKG74_01300"
     CDS             complement(3285..3824)
                     /locus_tag="PKG74_01300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOB89952.1"
                     /translation="MTILWILFFLTLAAWVMTTIVQIFNFWFSLDKRRCFYVPSKDKG
                     LIGIFKEEVSADKRYFYDLGSGDGRILRLAKKDFPDLEAVGYELNPILSSYSRILNRI
                     SKIDIQILRSDFLEANLQNNSIIYIFLRPQLLLELKNSFSALKNSVIVSHGFPIPYLE
                     NRLTKKVYGEPFNTYIYRL"
     gene            3928..4155
                     /gene="rpsO"
                     /locus_tag="PKG74_01305"
     CDS             3928..4155
                     /gene="rpsO"
                     /locus_tag="PKG74_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOB89953.1"
                     /translation="MLTPRKKSIIVKTKGIHETDTGSIQVQVGLLDKQIDELTAHLKK
                     HPKDVHSRRGLLKMVSKRRKLEHYLAAKTAK"
     gene            4157..4699
                     /locus_tag="PKG74_01310"
     CDS             4157..4699
                     /locus_tag="PKG74_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOB89954.1"
                     /translation="MAFIKHSDQRIGIFIDIQNLYHSARNLYGARVNYSELIKNLTDG
                     RKLIRAIAYVVSSDPQTGEELFFEALEKTGIELRSKEIQIFPDGTKKADWDVGMAVDA
                     IRLADSLDVIILVTGDGDFLPLVHYLKWGFGKAVEVAAFSKTASGKLKEEADGFYDLE
                     KMPRITFRSSRSKKPIPRNR"
BASE COUNT         1267 a         1057 c         1143 g         1271 t
ORIGIN      
        1 atcatggaga atttcttgag gatacctctt ttgccgaact aattgcttct tttgatgggg
       61 caattgagct ttttgtctgg ttcgagctat ccgccacaga ctgtccgaca atttcaccgt
      121 aagcggcgta ttcagccaat aaatatccaa atgttcgact tacttaaaat attctttatc
      181 aaaaaagcga tagccaatgg cggtaatctt cctacgcctg ccggagaagg cagtcttcct
      241 tcatccgtcg gcggcggcag tctttcttcc ggagaatctg ccggcggcgg gaatctattc
      301 gatccaggaa agtttcttcc gggagggctt ccgaccgatc tcggggcttt aatagaaacg
      361 atttttagat gggttttatg ggccgctacg cttgtttttc tttatcaggc ttttcgggcg
      421 gcctgggact tgataaccaa aaaagaagct gaaaccgcct tgagggatgc caaaaaaaga
      481 ttatggaatg ccttgatcgg atttatgatt cttctgtttg cccaaagcat tccgggaatc
      541 atcagggata ttttgaccgg ctgatttgcg gataagtacg gggaatttgt ataatagtat
      601 caatataaaa caaaaaaggt cataaaaaga actcgcaatg aaaaagatta attactcgac
      661 ggtagtggcg gccattgccg caaacacgac tattgctttc gccttggtgc tgccaccgat
      721 aattcctggt gtcggaattc ttgatccgaa ggcttggagt tgggcggaga tcgccagctg
      781 gccaaacatt attttaaact ggtttttttg gctaattact atttggacgg tctggatgat
      841 cttgcgaacg gcgcttgatt ttgttaattc ggccggagat ccaaaaaaaa tgggagaact
      901 ttcgacaaga gggcgcaata tcgctattgg cgttatcttg gctatacttg ccaggtccat
      961 accggctatt atctccacta ttattagcag tagcggtcaa aattaagaaa acaaataata
     1021 gataagaata acccccgcag atttctccgc gggggttgtg gttctacaaa gttattactg
     1081 caggaggaga agatgatggc ggggtaagga tgatggtgat ttcctcaccc gcgggacccc
     1141 agaaggtcgg ggtgccggtc ttaatacccc actcccccct ccaagagacc cttttccctc
     1201 ccgggaactg gacttctacg tccagtgtct cgggatagaa gtcgaacctc caccctggcc
     1261 actttgctgc cagggtgtta aatgatgggc cttcgactcc tttgtcgcct tgcaggacaa
     1321 tggtaatttc taccatttcc ttgctttctc ggattttata cccgggatcg ggtacttccg
     1381 aataaggtgg cggtgttatc ggcgccgtca ctcggtcggc aagcttgact gggctgaatt
     1441 tggctacagc catcatagcg atgaccatag caaacacaac agcccatctt ttattcacgt
     1501 ttgttaagcg tagaataaag tgggtcagtg tacctatcag cccagcaacg agaatggccg
     1561 aagtccaacc cttcggccct acaatcgcca tgattattgt aatagtaatc atggcgattg
     1621 tatctagaat gttgccgatc gctttcgcct tcaacgtctg ccaagtcgtt tgggacactc
     1681 ttctctcctc cttaagccct atcagggctt tatttctaat ctaaaagttc aaacttatat
     1741 ttatattata tcacattttg tgccttctgt caataaaata gctttattca tggcaaaatc
     1801 aggcatttta cgctataaac aagataagcc gggatggcga aactggcaga cgcgcagcgt
     1861 ttagggcgct gtgtccgcaa ggacttgagg gtccaaatcc ctctcccggc accgcattat
     1921 agaagtatga aaaagaggct tgacaaaatg ggcttattat ttatattaaa atcgtaaact
     1981 gccccctgat aattttataa ggggagacta gcagaaagga ggaataggtt gtgttgaaga
     2041 aagagggaga tgttatcatc cacgccaaag aacatgagtc ctcttttggt ttgggagatc
     2101 ttctcctcgc ggccgtgacc ggaggagtgt cattatttct tccggatccc aaatatgaag
     2161 ccgaagctct tagtattagc actaacacca gcgctaaggg ttatggcgat actaaggcag
     2221 aggccgagat gaacgcgggg aaagaactag ccaaagaatt ggagaagcgc cggccataag
     2281 gttgcgtgcc acgccggact cgaaataaaa ctcaagtccg gcgttgtttt ttatttaata
     2341 acttttagtt tctattctct ccgccttccg tgaaaagcgt cgtgaatttc ttttaaagtt
     2401 ttatttgtaa ggtgagtata aatttgcgtt gtctggatgc ttgcatggcc gagcatttcc
     2461 tggaccgatc tcaaatccgc tccgttcatc aataagtcgg tcgcgaaaag atgtcgtaaa
     2521 gtgtgcggag atactttccg ggagactccg gctttgcggc tgtaaaagta taccaaacgt
     2581 tccacgcttc gcggcgttat ccgtccgatg acacgcggat tgctgccgtt cgaataactg
     2641 acgaaaagcg cctcctcggc gtcggctctt ttgtcgagat aatttttgat cgcttttttg
     2701 gcgcggtcgg aaataaaaac cactcgcagt ttttctcctt tgccgcgaac cgtgatttcg
     2761 ccgcgcttcc aattgagata ccggtcgagg gaacagagtt cggaaacgcg gagcccggtc
     2821 gagaaaagca gctcgagaat ggcccggtcg cggagggctt tcagggtcga tccggccgga
     2881 gcttcgagaa tcctttccag atcttcgtat tcgatgatgt cgatctgcct tttagggatc
     2941 tgcggcagtt cgattctgtc cggcgaaacg actttcacgt ccatcttgac gagatagcgg
     3001 agaaaattcc tgatggcgat gatgcggtag ctttgggtgt tttttttgag atcggttttc
     3061 tccgcgagat aaacgcggta agaacggatc ttatcgttcg tgatatccgc gggttttttg
     3121 attttggaga attcgaagaa ttttttgagc gcccgttcgt aaacgtcttt ggttcgggga
     3181 gagcggtttt tctcgatttg gagataatcc aggtagtctt tcaggtattt ctcgatcatc
     3241 gattgattct accacgtctg tcgcaaaatg tgcgacgatg cgattcaaag acgatagata
     3301 taagtattaa aaggttcgcc gtagactttt ttcgtcagtc tgttttcgag ataaggaatg
     3361 ggaaaaccgt gcgaaacaat gacggagttt ttcagagccg aaaaactatt tttaagctca
     3421 agcaataatt gaggccggag aaaaatatag attattgaat tgttttgcag attcgcttcc
     3481 agaaaatcgc tccggagaat ttggatatct attttggaaa tccggttcaa aatccgggaa
     3541 tacgaagaga gaatcggatt aagctcgtag ccaacagctt ccagatcggg aaaatccttt
     3601 ttggccaaac ggagaattcg gccgtcgccg gatcccaggt cgtaaaaata gcgtttgtcg
     3661 gccgacactt cttctttgaa tattccgatc agccctttat ctttgcttgg tacataaaaa
     3721 caccggcgtt tgtccaaact gaaccaaaaa ttgaaaatct ggacgattgt cgtcatgacc
     3781 catgcggcaa gcgtcaaaaa gaacaaaatc cacaagatag tcattagaaa caaaggcatt
     3841 atccttattt tagatcattt atcggttttc cggagccctt gaaaaaaatc ggattccggg
     3901 ttaatcttaa gaaaatcaaa acaacttatg cttacacccc gcaaaaaatc aataatcgtc
     3961 aaaaccaaag ggattcacga aaccgacacc ggatcgatcc aggttcaggt cgggcttttg
     4021 gacaagcaaa tcgacgagct gaccgctcat ctgaaaaaac atccgaaaga cgtccattcc
     4081 cgccggggct tgctcaaaat ggtgagcaag cggcgcaaac tcgagcatta tctcgcggcc
     4141 aagaccgcca aataaaatgg cttttataaa acattctgac cagaggatcg gcatttttat
     4201 cgatatccaa aatctttatc actccgcgag aaacctctac ggagcgagag ttaattattc
     4261 cgaacttatc aagaatctga ccgatggccg gaaattgatc cgggccatcg cctatgttgt
     4321 ttcgagcgat ccccagaccg gggaagagct ctttttcgag gctttggaaa aaaccggaat
     4381 cgagctccgg agcaaggaaa tccagatttt cccggacggc accaaaaaag ccgactggga
     4441 cgtgggcatg gccgtcgacg ccatcaggtt ggcggactcg ctcgatgtca ttatcctcgt
     4501 gaccggagac ggggattttc tgccgctcgt ccattatctc aaatggggat ttggtaaggc
     4561 cgtggaagtg gccgcttttt caaagaccgc ttcgggcaag ctcaaagaag aggccgatgg
     4621 attttacgac ttggaaaaaa tgccgcggat aaccttcaga tcgtcgagat cgaaaaaacc
     4681 aataccaaga aaccgctaac atggaactga aaaagaaagt ttttacaaag aatatcgc
//
LOCUS       DAOPQT010000033         4540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_20718_length_4540_cov_3.449275, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000033 DAOPQT010000000
VERSION     DAOPQT010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20718_length_4540_cov_3.449275"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2287)
                     /locus_tag="PKG74_01315"
     CDS             complement(<1..2287)
                     /locus_tag="PKG74_01315"
                     /inference="COORDINATES: protein motif:HMM:NF027952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HOB89955.1"
                     /translation="MKSDLKKVLSIFLVGVVLCGSVFLSSLWLKYASAVDGSGTNTVN
                     PTTTTASSTGNEFDFTYTASETMDSGGINITVPAGWSSPQGTSGVPGYTTAASTGIIA
                     NQKNSLDSSAGWAVTNHMSLSSDTVDKQVGVASLSNNITSSAAANEQWYFNYGSAENW
                     GSSVSGNLRTGFFLKSSVATSAGNLHWQNDDSANLASPNDTISLPALAANTWTYTSAT
                     LGAAARNSILSYGFRYTTDIGAAVVKADNISVIFQSNDSNTGWSGDTGINDSLITGIE
                     GTGAVRCTYAGNAGTGANGDCFNEEAAVVTVGPGTTVSFFVRPSIALNAGDFAWVDDN
                     SSNVGSPDSVVSLPALPANVWTYVTVTSSSSGNMRSFGLRQLVDRGALTIDIDAIGKV
                     IDTCDSTSGWTAPTSTVQTLAIDNSIFHEGTGSLRNTIASTAAPGDKWYESLGSAENW
                     SGYTTAGFWIRSSVAVSAGQLKFEYASSSNLTSPVASIDIGALSANVWSYQKLTLTGT
                     RNSVSSYGINYSTDIGSATIYLDDILLGPGSISFPGNDINIRLLSLASGQTVTVNYGA
                     GGGASGVTAPSTPGVYIFDTKSRSDDSGTLANILVSPTITVRQTTTTTLETSGSPSVY
                     GTPITFTATVTPTTGGAPSGTVTFRNGETIIGTGNLNGANPGVATLILNSLTVSGSPH
                     SITATYGGNSIYLSSVSGVVSQTITPKNLTVIGITANDKTYDGNTSAVLNVTGAALVG
                     VVSGDNVILNTMNAVGTFDTPA"
     gene            2345..2881
                     /locus_tag="PKG74_01320"
     CDS             2345..2881
                     /locus_tag="PKG74_01320"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOB89956.1"
                     /translation="MMIKSLPLSEVYRLLEPGPVVLVATADKNKNNVMTMSWHTMMEF
                     EPPLIGCVISGRNHTFDTLLKTKECVIGIPTAKMIDKVVKIGNCSGRDTDKFKKIGLT
                     PLPASRVKAPLVKECYANIECKISDANLVGKYNFFILKAVKAWIDPKAKNPKIIHHGG
                     KGNFTISDKAVKIASKMK"
     gene            2955..3632
                     /locus_tag="PKG74_01325"
     CDS             2955..3632
                     /locus_tag="PKG74_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOB89957.1"
                     /translation="MNRKKIVLCVTLLVLAVIAVFLAIRARQLAFASYSDGSTTVKAV
                     FDNKKDTVTFTHESTGKVMLPIAISASGARYANADESIVFWEHQGEATIFKDDATVFQ
                     GRIQNTAAKKALSASTWVWEQTIMNDDSVIVPQRTNAFTLKFSTDGRVSGTTDCNSFS
                     GSYEAWSDGAISFSPFASTLMFCEGSQESQFVSAVSDSDRYLMDDAGNLVLLIKFDSG
                     QVHFRRQ"
     gene            3759..4031
                     /locus_tag="PKG74_01330"
     CDS             3759..4031
                     /locus_tag="PKG74_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOB89958.1"
                     /translation="MNTEKQKTLTGFRKAQSLLKKIIDMAEKDSYCIDIMQQNLAVIG
                     LLRAANQTLMQGHLNSCFRSAMAAKNEKRKTEMIEEILSVTKIFNR"
     gene            4033..>4540
                     /locus_tag="PKG74_01335"
     CDS             4033..>4540
                     /locus_tag="PKG74_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOB89959.1"
                     /translation="MAKKTFYVKGMHCASCEVLVEKELLSIPGIVFADASLVRSSVNL
                     EYDKESPSLEKLNAKFEPLGYSFSKEAFEEKSNGWKIPLIALSAIAIFLVISKSGLSS
                     LINVSSESSLTAFFVFGFIAGISSCAALIGGLILSLSKKWLEDFGRGGGFTEKAKPHL
                     LFNLGRLIS"
BASE COUNT         1143 a         1175 c         1174 g         1048 t
ORIGIN      
        1 cggccggggt atcgaaagtt ccgacggcat tcattgtgtt caagataacg ttgtctccgg
       61 atacgacacc caccaaagcg gcacccgtta cgttcaagac agccgaagtg tttccgtcat
      121 acgtcttgtc gttcgccgtg atgccaatga cggtcaggtt tttcggagtg atcgtctggg
      181 agacgactcc cgaaacgctt gataaataaa tgctgtttcc cccgtaggtc gccgtgatcg
      241 agtggggaga accggaaacc gtcaggctgt tcaatatgag agtggcgacg ccggggtttg
      301 cgccgttcaa attacccgta ccgatgatgg tttcgccgtt cctgaaagtg actgttccgc
      361 tcggcgctcc tccggttgtc ggcgtaacgg tcgccgtaaa agtgatcggc gtgccgtaga
      421 ccgaaggact acccgaagtt tccagagtgg tcgtcgtggt ttgtcttacg gttattgtcg
      481 gagaaaccaa gatattggcc aaggttccgg aatcgtcgct tctcgacttg gtatcgaaaa
      541 tatagacgcc gggagtggaa ggcgcggtta cgccgcttgc tccgccgccg gctccgtaat
      601 tcacggtaac cgtctgcccg gaggcaagag acaaaagccg gatattgata tcgttcccgg
      661 gaaaagaaat actgcccggc cccaagagaa tgtcgtccaa atatatggtc gcactgccga
      721 tgtcggtcga atagttgatg ccgtagcttg aaacggaatt tctcgttccg gtgagcgtca
      781 atttctggta actccaaaca ttggcggata aggctccgat atcgatcgaa gcaactgggc
      841 tcgtgagatt ggaagacgaa gcgtattcga atttgagttg tccggcggac acggcgaccg
      901 aggaacggat ccaaaatccg gcagtcgtat aaccggacca gttttccgcc gagcccaaag
      961 attcgtacca tttgtctcca ggggcggccg tcgaagcgat tgtgttgcgg agcgatccgg
     1021 tcccttcgtg aaaaatggaa ttgtcgatcg ccagggtttg caccgtcgaa gtgggcgcag
     1081 tccaacctga cgtcgaatcg caagtatcga tgacttttcc gatggcgtcg atgtcgatcg
     1141 tcaacgctcc cctgtccacg agctgacgca gaccaaaaga acgcatgttt ccggaagaag
     1201 acgaagtaac ggtaacgtat gtccagacgt tggccggaag agccggtaga ctgacaacgc
     1261 tgtccggcga accgacgttc gaggaattgt cgtcaaccca ggcaaaatct ccggcgttca
     1321 aagcaatgct cgggcgcacg aaaaagctga ccgtggttcc cggaccgact gttacgaccg
     1381 ccgcttcttc gttgaaacaa tcgccgttcg cgcctgtacc ggcgtttccg gcgtaggtgc
     1441 agcgcaccgc tccggttcct tcgattccag taataagaga atcgttaatg ccggtatcgc
     1501 cggaccagcc ggtattcgaa tcgttggatt ggaagataac ggaaatgtta tccgctttga
     1561 cgactgccgc gccaatgtcg gtcgtatagc ggaagccgta agaaagaatg gaatttctgg
     1621 cggcggctcc gagcgtggcc gaggtgtagg tccaggtgtt ggccgcaagc gcgggaaggg
     1681 aaatcgtgtc gttcggactg gcgaggttgg cggaatcatc attttgccaa tgaaggtttc
     1741 cggccgacgt ggccacgctc gatttgagga aaaatcccgt acggagattg ccgctcacgc
     1801 tcgagcccca gttttccgcc gaaccgtaat tgaaatacca ctgttcgttg gcggcggcgg
     1861 atgaagtgat gttgttcgag agtgaagcaa cacccacctg tttgtccacg gtgtcggaag
     1921 aaagactcat atggttcgtt accgcccagc cagccgaaga atcaagactg tttttctgat
     1981 tcgctatgat gccggtcgag gcggcggtcg tgtatccggg aacgccggac gtcccttgtg
     2041 gagagctcca ccccgccggg acagtgatgt ttattccgcc ggaatccatg gtttccgaag
     2101 cggtataagt gaaatcaaac tcgttgccgg tggaagaggc ggtggtcgtc gtcgggttca
     2161 ccgtattcgt gccgcttccg tcgacagccg aagcgtactt cagccaaagg ctggataaaa
     2221 aaacagagcc gcacaaaacg actcctacaa ggaagattga taaaactttt ttaagatcgg
     2281 atttcatcct tacttactta ttataaagta taacattttc ggcgcgcaaa tatattataa
     2341 tggaattatg atcaaatctc ttcccttgtc cgaggtttat cggctgctcg agcccggacc
     2401 ggtggttctc gtcgcgactg ccgacaaaaa caagaacaac gtcatgacca tgtcgtggca
     2461 cacgatgatg gaattcgaac cgcctttgat cggctgcgtc atcagcggcc ggaatcatac
     2521 cttcgacacc ctcttgaaaa ccaaagagtg cgtcatcggt attcccacgg ccaaaatgat
     2581 cgacaaagtc gtcaagatcg gcaattgttc cggtcgggat acggataaat ttaagaagat
     2641 cggcctcact cctctccccg cttcccgcgt caaagcgccg cttgtcaaag aatgctacgc
     2701 caatatcgaa tgcaagatca gcgacgcgaa cctcgtcggc aaatacaact tctttattct
     2761 aaaagcggtc aaggcttgga tcgacccgaa agcaaaaaat ccgaaaatca ttcatcatgg
     2821 cggcaaagga aacttcacca tatcggacaa agccgtcaaa atcgcctcga agatgaagta
     2881 gcccgccttt attcttccat ttataataaa tacagcgagg ctctgtcggg ccttaaatac
     2941 aaataaaaca aaacatgaat cggaaaaaaa tagttttatg cgttaccctg ctcgtcttgg
     3001 ccgtcatcgc cgtctttttg gcgatccggg caagacagct cgcattcgct tcgtattccg
     3061 acgggtcgac cacggtcaag gcggttttcg acaacaagaa agataccgtg acctttaccc
     3121 acgagagcac gggaaaggtg atgcttccga tcgccatatc cgctagcggc gcccgctacg
     3181 ctaacgccga cgaaagcatc gtcttttggg aacatcaggg agaagcgacc atcttcaagg
     3241 acgacgcgac ggtcttccag ggccgcatcc aaaataccgc cgccaaaaaa gctctgtccg
     3301 cttcgacctg ggtttgggaa caaacgatca tgaacgacga ttccgtcatc gttccccaga
     3361 gaacgaatgc cttcactctc aaattctcta ccgacggccg ggtttccgga accaccgatt
     3421 gcaacagttt cagcggaagc tacgaagcct ggagcgacgg agccatcagt ttcagtccct
     3481 tcgcctcaac tttgatgttc tgcgaaggat cgcaggaaag ccagttcgtg tccgcggttt
     3541 ccgattccga ccgttatctt atggatgatg ccggaaatct ggtgttgctc atcaagttcg
     3601 attcgggtca agtccatttc aggaggcaat agatcggcaa aacgcaaaac cccgtcatcc
     3661 agacggggtt tttagcgatt gacagcttta tctaccgctg tattattaac ctatacccca
     3721 ggtataggtc gcaataaaaa ccaacgccgt aaaccgatat gaacaccgaa aagcaaaaaa
     3781 ctctgaccgg attcagaaaa gcgcagagcc tcctcaaaaa aatcatcgac atggccgaaa
     3841 aagactctta ttgcatcgac atcatgcagc agaatctcgc ggtcatcggc cttttacggg
     3901 cagcgaatca aacgctcatg cagggtcacc tgaacagctg ctttagatcc gccatggccg
     3961 ccaagaacga aaaaagaaaa accgagatga tcgaagagat cttgagcgtc accaagatct
     4021 tcaatcgctg aaatggccaa aaagactttt tatgtcaaag gcatgcactg tgcttcctgc
     4081 gaagtgcttg tggagaaaga acttctttct attcccggaa tcgttttcgc cgacgcttcc
     4141 ctggtccgaa gttcggtcaa tctggaatac gataaagaaa gtccgagcct cgagaaactg
     4201 aatgccaaat tcgagccctt gggctattct ttctccaaag aagctttcga agagaaaagc
     4261 aacggatgga aaataccgct tatcgctctt tcggcgatag cgattttctt ggtgatttcc
     4321 aaatcgggat tgtcttcttt gatcaatgtt tcttcggaaa gctctttgac cgcttttttc
     4381 gttttcgggt tcatcgccgg aatatcgagt tgcgccgccc tgatcggcgg actgatcctc
     4441 tccctttcca aaaaatggct cgaagatttc ggaagaggcg gcggttttac ggaaaaagcc
     4501 aaaccgcatc ttcttttcaa tttgggacgc ttgatttccc
//
LOCUS       DAOPQT010000034         4447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_21305_length_4447_cov_2.782787, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000034 DAOPQT010000000
VERSION     DAOPQT010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21305_length_4447_cov_2.782787"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKG74_01340"
     CDS             <1..391
                     /locus_tag="PKG74_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOB89960.1"
                     /translation="PRLRDAVLKAKEASVPQENIDRAIKKAKDEPSEELLVEMYGPEG
                     SAFIVTGLTSNVNRTMADIKAVAKSFDAKIGLPGSAKWAFSQSGFEFVPNFEQAVSPE
                     SKEKILALIEALEEIEEVGAVYTNAES"
     gene            388..873
                     /gene="ruvC"
                     /locus_tag="PKG74_01345"
     CDS             388..873
                     /gene="ruvC"
                     /locus_tag="PKG74_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOB89961.1"
                     /translation="MKTLKVLGIDPGSHRIGFGLVEKSGRELSAKDYGVIEFKSGETS
                     DRLMLLKKEMEKLIEKTKPSIVAVERLYFSQNRTTGMMVAESRGIIMLSAKKANIPTV
                     EYDPTTVKLRVTGYGLSDKKAVMKMVKILLNLQNFNGIDDASDALAIAIVACLDNREI
                     G"
     gene            958..1131
                     /locus_tag="PKG74_01350"
     CDS             958..1131
                     /locus_tag="PKG74_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89962.1"
                     /translation="MTMTDYYNKGYLLMDIGGEGEGDSDDFDAFSKSEEDVEDDEESE
                     DEEEEEESDDTEW"
     gene            1202..3676
                     /locus_tag="PKG74_01355"
     CDS             1202..3676
                     /locus_tag="PKG74_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOB89963.1"
                     /translation="MAKIKVRRVKKASVGKDVFISVVALGVFVVFAGVFAFYRLSKSL
                     PPVAQLDGNVKTESTKIYDRTGQTLLYEIYDEERRTILPSEEIPEIMRQAVIAVEDKD
                     FYNHPAFDIKGMIRALVADILNQNKSQGGSTITQQLDRNAFLTTERSIARKVKEIILA
                     YRIEKLYSKDEILDLYLNQIPFGQNSYGIEAASETYFAKHAPELSLSEAALLAAVAKG
                     PSYFSPWGSHLVELEGRRQYVLEQMELVGFIDKQQLEAARADKPKVLAEPQKANFEIA
                     PHFVIYVQDYLNQKYGEDFVRRSGMKVVTTLDVDLQKLANEKIKAGGERNIEAINGHN
                     LALIAEDATNGQILAMVGSRDYFADSEPAGCIEGSTCLFEGKFNAASQSLRQPGSALK
                     PFIYSLAFKSGLTPDTVVFDVPTEFAAKNPLCPITPAFFSDNKQCYHPGNYDGLFRGP
                     IMLKDALARSLNVPAVKVLHTVGLQNAINWLEKFGVTTLNDPDRIGLSLTLGGGEIRL
                     TELTNAYATLANDGVFNPQTAILSITDSKGNVLEEFESRSERVVDEQYARLVNDILSD
                     LNLREPLFRSNLYMTQVPGKQIALKTGTSQDYVDAWAFGYSPRLVVGLWAGNNNRYPL
                     GKAGSSILAAIPTWHDFAAEAFKNEPSLTFIKPQPIYTDSPILLGAIDRNNVHDLLYF
                     LNRISDSQYPNWEEAVRSWLSLNSMPLYWGQATYTFPNDGQAALNGEKNITIDLVYPK
                     NGDFVDAMSEIRGSVRSESGVNNIELSINGQVIDSRMINAEKSVDYRFVLGGAPLNPQ
                     NSLKLRVTNILGNSAETEIIVFKKTD"
     gene            complement(3696..>4447)
                     /gene="dnaN"
                     /locus_tag="PKG74_01360"
     CDS             complement(3696..>4447)
                     /gene="dnaN"
                     /locus_tag="PKG74_01360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOB89964.1"
                     /translation="APEAGYFEISRSVLKEAINKIINACQISDLRPEISGILFNFESN
                     VFKLVATDSFRLAEKSIPETQFKNNLSKSGKTIIPLKTIQEIVRILNDEESVKVFIDD
                     SQALFKTETLEVITRLIDGQFPDYGPIIPKALETEIVVEKQELVNAMKLAGSFTARSG
                     EVVIKVGDDKKIVEVYSADSGLGENKYLISAKISGDPVKASFNWKYLLDGLKNIYTDT
                     VLFGLQGDSRPAIMKAPNDTSYFYILMPIKN"
BASE COUNT         1163 a         1140 c         1095 g         1049 t
ORIGIN      
        1 tccgcgtctc cgcgacgccg tgctcaaagc taaagaggcg agcgttcccc aagagaatat
       61 cgaccgggcc attaaaaaag ccaaggacga gccgagcgag gaactcttgg tcgaaatgta
      121 cggacccgaa ggatcggctt tcatcgttac tgggctcaca tcgaacgtca accggaccat
      181 ggccgacatt aaggctgtcg ccaagagctt cgacgccaaa ataggcttgc ccggttcggc
      241 caaatgggcc ttttcccagt ccggcttcga atttgttccg aatttcgaac aagccgtttc
      301 tcccgaatcc aaagaaaaaa tcctcgccct gatcgaggct ctcgaagaaa tagaagaagt
      361 gggtgccgtt tacacgaacg ccgaatcatg aaaaccctta aggttttggg gatcgatccc
      421 ggaagccacc gcatcggctt tggtttggtg gagaagtccg gccgcgaact ctcggccaaa
      481 gactacggag tgatcgaatt caagtccgga gaaacttcgg acagactaat gcttttgaaa
      541 aaggaaatgg aaaagcttat tgagaagacc aagccgtcga tcgttgccgt ggaaaggctt
      601 tatttttccc aaaaccggac caccggcatg atggtcgccg aatcgcgcgg gatcatcatg
      661 ctctccgcca aaaaagccaa tatcccgacc gtcgaatacg atccgacgac agtaaagctg
      721 agagtgaccg gctacggatt atccgacaaa aaggccgtca tgaaaatggt gaaaatcctt
      781 cttaaccttc aaaacttcaa cggaatcgac gacgcctcgg acgctttggc gatcgcaatc
      841 gtcgcctgcc tcgacaaccg cgaaatcgga taatagcccg gacccttgac gcccgggaaa
      901 aaataattat actttttcaa gttatcaaaa tataaagcaa aaaggtcgaa actttttatg
      961 acgatgacgg attattacaa taaaggatac cttttgatgg atatcggcgg agaaggagag
     1021 ggtgattccg acgatttcga cgcattttcc aaatccgaag aagatgtcga ggacgacgaa
     1081 gagtccgaag acgaagaaga ggaggaagag agcgacgaca ccgagtggta aaaagcaatc
     1141 tatatctata atgatccgcc ttggttataa taaagaccag ggcggatttg gttaatgagt
     1201 catggctaaa ataaaagtaa ggagagtaaa aaaagccagc gtcggaaaag acgtctttat
     1261 ttccgtcgtc gctttgggtg tttttgtggt tttcgccggt gtctttgctt tctatcgtct
     1321 gagcaagagt ctgccgccgg tcgcccagct cgacggaaac gtcaagaccg aatccaccaa
     1381 aatctacgac cgcaccggtc agacgcttct ttacgaaatc tatgacgaag agcggcgaac
     1441 cattcttcct tcggaagaga ttccggagat catgaggcag gcggtcatcg ccgtcgaaga
     1501 caaggatttc tacaaccacc cggctttcga catcaaggga atgatccggg cgcttgtcgc
     1561 cgacattttg aaccagaaca aatcccaggg cggatcaacc atcacccagc agctcgaccg
     1621 gaacgctttt ttgacgaccg aacgctcgat cgcgagaaaa gtcaaagaga tcatcctcgc
     1681 ttaccgcatc gaaaaacttt attccaaaga cgaaatactc gatctttatt tgaaccagat
     1741 tccttttggc cagaacagct acggcatcga agcggcgagc gaaacctatt tcgccaaaca
     1801 tgctccggaa ttgagccttt ccgaagcggc cctgctcgcg gccgtggcca agggtccgag
     1861 ttatttctct ccctggggat cgcacctcgt cgaactcgag gggcggaggc agtacgtatt
     1921 ggagcaaatg gaattggtcg gttttatcga caagcagcaa cttgaagcgg cgagagccga
     1981 caagccgaaa gtcctcgccg aaccgcagaa agccaatttc gagatcgctc cccatttcgt
     2041 gatctatgtt caggattatc tgaaccagaa atacggcgag gattttgtca ggcgctcggg
     2101 catgaaagtc gtcaccactc ttgacgtcga tctccaaaaa ctggcgaacg aaaaaataaa
     2161 agccggagga gaaagaaata tcgaggccat caacggccat aacctggctt tgatcgccga
     2221 agacgcgaca aacggccaaa tactcgccat ggtcgggtca agggactatt tcgccgactc
     2281 cgaacccgcc ggatgtatcg aaggatcgac ttgtcttttc gagggtaagt ttaacgccgc
     2341 gagccaaagc ctgcggcaac cgggatcggc tttgaagccc tttatctatt ccttggcttt
     2401 caagtccgga ctgactccgg atacggttgt tttcgacgtt ccgaccgaat tcgcggccaa
     2461 aaatccgctc tgtccgatca ccccggcttt tttcagcgac aacaagcagt gctatcatcc
     2521 gggtaattac gacggtcttt tccggggacc gatcatgctg aaagacgccc tggctcgctc
     2581 tttgaacgtg ccggcggtca aagtactcca cacggtcggg ctccaaaacg ccatcaattg
     2641 gcttgagaaa ttcggagtga ccactctgaa cgatccggac cgcatcggac tatccttgac
     2701 tcttggagga ggggagattc ggctcaccga gctcacgaac gcctatgcca cgctcgcgaa
     2761 cgacggagtt ttcaatccgc aaaccgcgat cttgagcatc accgattcca aaggaaatgt
     2821 tttggaagaa ttcgaatccc gttcggaaag ggtggtcgac gaacaatacg cgagactcgt
     2881 gaacgatatc ttgtccgacc tgaatctccg ggagccgctt ttccggtcaa acctttacat
     2941 gacccaggtt cccggaaaac aaatcgccct gaaaaccgga acttcccagg attatgtcga
     3001 cgcctgggct ttcggatatt ctccgcgact cgtggtcggt ctttgggccg gcaacaacaa
     3061 ccgttatcct ttgggtaagg ccggatcgag tattttggcc gccattccca cttggcacga
     3121 tttcgcggcc gaggctttca agaacgaacc cagcctcacc tttatcaagc cgcagccgat
     3181 ctataccgac tcacccatac ttctcggagc gatcgaccgg aacaatgtcc acgatcttct
     3241 ttattttttg aacaggattt ccgattcgca atacccgaac tgggaagaag ccgtcagatc
     3301 ctggctttcc ttgaacagca tgccgcttta ctggggacag gccacttata cttttccgaa
     3361 cgacggacaa gcggctttaa acggagaaaa aaacatcacc atcgatctcg tctatcctaa
     3421 aaacggggat tttgtcgatg cgatgtcgga aatcagaggt tctgtccgtt cggaatccgg
     3481 agtgaacaac atcgaacttt cgataaacgg acaagttata gacagccgga tgattaatgc
     3541 ggagaaaagc gtcgattatc gctttgttct aggaggggct cctttgaatc cccaaaactc
     3601 tctaaagtta agagtgacaa acattctcgg caactcggcc gaaacagaga ttattgtttt
     3661 caaaaagacc gattaaatcg gtcttttttg cttgatcagt tcttgatcgg catcaagata
     3721 tagaaataag aggtgtcgtt cggcgctttc atgatcgccg gccgactgtc tccctggagt
     3781 ccgaacaaaa ccgtgtccgt ataaatgttt ttcagtccat cgaggagata tttccaattg
     3841 aacgaggctt ttaccggatc tccgctgatt ttcgcggaaa tgaggtattt gttttctccg
     3901 agcccgctgt ccgcggaata aacctcgact attttcttgt cgtctccgac cttgatcacc
     3961 acttcgccgc ttctcgcggt gaatgatccg gccagcttca tggcgttcac caattcctgt
     4021 ttttcgacga cgatttccgt ttccaaagcc ttggggatga tcggcccgta atccgggaat
     4081 tgtccgtcga tcaaccgagt gatcacctcc agggtttcgg ttttgaaaag agcctgggaa
     4141 tcgtcgataa atactttgac gctttcttcg tcgttcaata ttctcacgat ttcctgaatg
     4201 gttttcaggg ggatgatggt ttttcctgat ttcgacagat tgttcttgaa ttgcgtttcc
     4261 ggaatgcttt tttccgccaa tctgaagctg tcggtcgcca ccagtttgaa gacgttcgac
     4321 tcgaagttga aaagaatgcc gcttatttcc ggacgcagat cggaaatctg gcaagcatta
     4381 atgatcttgt ttatcgcttc ttttaggacc gatctggata tctcgaaata tccggcctcc
     4441 ggggcga
//
LOCUS       DAOPQT010000035         4377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_21756_length_4377_cov_3.333873, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000035 DAOPQT010000000
VERSION     DAOPQT010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21756_length_4377_cov_3.333873"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..496
                     /gene="raiA"
                     /locus_tag="PKG74_01365"
     CDS             182..496
                     /gene="raiA"
                     /locus_tag="PKG74_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOB89965.1"
                     /translation="MNINLKASGFKLTPAIRENVSTKLKSVEKLISKFDENGEIELDF
                     EVSKTTRHHQKGNVFRAEANLNLPKKLIRAEAVEEDLTAAIDKVKSILTLSVKKYKEL
                     NS"
     gene            496..2910
                     /gene="secA"
                     /locus_tag="PKG74_01370"
     CDS             496..2910
                     /gene="secA"
                     /locus_tag="PKG74_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOB89966.1"
                     /translation="MFFKFLSGARGRDPKRTIAEINGAEEKTASLSDAEIKEKSLALR
                     ERIIKGENPEAAIPEAFALAREASRRTLGQRPFDTQMLGGLHLHYGKIIEMLTGEGKT
                     LAAVAPAYLNALSEKGVHIITVNDYLAKRDAVWMGQIYHALGLKTACIVHDSAFLYDP
                     SFVPSHAAESIDEERDIKGSFLIQDKYLRPISRKEAYLADVTYGTNAEFGFDYLRDNL
                     AYDALSQVQRPHYFAIIDEVDSILIDEARTPLIIAAPDQSSSDYYRVFSKAVKQLVEG
                     EDYTADDKTKSVEITDKGIDKVEKLVGVHDLYEPQNFRLVHYLEACLKAQRHFLLDRD
                     YVVRDGEVVIVDQFTGRLMFGRRYSAGLHQALEAKEGLKVADESKTYAQITIQNYFRL
                     YQKISGMTGTAQTSAEEFFKVYKLETVSIPTNKPMIRKDLPDLIYKTKEAKYQAIVED
                     IRTRREKGQPILIGTISIEKNEEFSKYLERAGIGHEILNAKNNEREGAIIAQAGRPGA
                     VTLATNMAGRGVDIVLGGNPPDKEAAEKVRQAGGLHVIGTERHEARRIDNQLRGRSGR
                     QGDPGSSRFFLSLEDDLMRIFGGDRLKSMMQTLKIPEDQPIEAGLVSKAIAEAQSKVE
                     GFNFDIRKNLLDYDDVLNKQRTNIYSHRQEILESNDEGTRKLIASAFEEAGAELPPDF
                     ENQLQSLDHLKEIRRVILMILDMFWMNHLEDIESLTESVRIRAYGQRDPLVEYRRDSH
                     MMFNAMVRNFNQWIADNIFKIKFEEAPLSEPASPQPVRFSGKKVGRNDPCPCKSGKKF
                     KHCCGK"
     gene            2915..3382
                     /locus_tag="PKG74_01375"
     CDS             2915..3382
                     /locus_tag="PKG74_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOB89967.1"
                     /translation="MKSSFKPKPGQVDYTHIRWAPVINCVLKHKNKILVVRRSEKLNF
                     YPGYWNGVSGFLDDQKSLEEKVVAELKEELGIAKTQVENIRPGGIFDQDEPKYKKTWI
                     VHPVLVDVKTDKIKLDWEAENYKWLTLQEVKKLKLLPGFDEVLRRLSPWIKNK"
     gene            3379..3681
                     /locus_tag="PKG74_01380"
     CDS             3379..3681
                     /locus_tag="PKG74_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011973699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOB89968.1"
                     /translation="MKYNKLVRDKIPEIIKESGGTPITHIAADVEYWLKLKEKLLEEI
                     KEFFENESIEELADVKEVLNAIRDYKGFSESELEATRVAKAEKRGAFEKRIILEES"
     gene            3690..4112
                     /locus_tag="PKG74_01385"
     CDS             3690..4112
                     /locus_tag="PKG74_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOB89969.1"
                     /translation="MEKGGMKEFYAGGFFYNPRTKSVLLHKRSPDAEFNPDKWAFFGG
                     LSENEETPIETWRREIKEELDVDLTEKQIKPLRDYYNKDRQQHRYAFFAETDLPKSEM
                     TLGEGADFDWIPLDEVFGYDLTAKTAEDLRFFIENFLE"
     gene            4118..>4377
                     /locus_tag="PKG74_01390"
     CDS             4118..>4377
                     /locus_tag="PKG74_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89970.1"
                     /translation="MDNNFKKSPVVKDVKNPGGEFEEKTFDGLFKGRGVSSFSRKRGR
                     ISPKTIFWGVFLLTVALGIGSFVHYRNQTMESVAELQKSFMK"
BASE COUNT         1278 a         1033 c         1121 g          945 t
ORIGIN      
        1 caagaagact aagaagagaa agtagtcttt gaaaaaaatc tccttcttcc gttcgcgggg
       61 gaaggagatt tttaatttag gggtgttttt tcgcctccag ccacggctcc aaacgcttat
      121 tggccgactt ttccgggtcc tgatataatg tggtctgaag gtcgcaagct tctttatata
      181 tatgaatatc aacctcaaag catccggctt caagctcacc ccggcaatcc gggaaaacgt
      241 atccaccaaa ctgaaatccg tcgagaaact gatctccaaa tttgacgaaa acggcgaaat
      301 cgaattagat ttcgaagtct cgaaaaccac ccgtcaccac cagaaaggca atgttttcag
      361 ggcggaagcg aatctgaatc ttccgaagaa acttatccgg gccgaagcgg tggaagaaga
      421 tttgacggcg gcgatagaca aggtcaagag catccttacg ctctcggtga aaaaatacaa
      481 agaattgaac agttgatgtt tttcaaattt ttatcaggcg ctcgcggccg cgacccaaaa
      541 cggacgatcg cggaaatcaa cggcgccgaa gaaaagacgg cgtctttgag cgacgccgaa
      601 ataaaagaaa agagcctcgc tttgcgcgag agaataatca aaggagaaaa tccggaggca
      661 gcaattccgg aagcctttgc tttggcgcgc gaggcatcca ggcgcaccct ggggcaaagg
      721 cctttcgata cgcagatgct cggaggcctg catctccatt atggcaagat catagagatg
      781 ttgaccggag agggaaagac tttggcagcg gtggctccgg cttatctgaa cgccctttcg
      841 gagaaaggag tccatatcat cacggttaac gattatctcg ccaaaagaga cgccgtctgg
      901 atgggccaga tttatcacgc cttgggctta aaaaccgcct gcattgtcca cgacagcgct
      961 tttttgtacg atccgtcttt tgttccgagc catgccgccg aatccattga cgaggagcgc
     1021 gatatcaaag gaagcttttt gatccaagac aaatacttgc ggccgatcag ccgcaaagag
     1081 gcgtatttgg ccgacgtcac ctacggcacg aacgccgaat tcggattcga ctatttgaga
     1141 gacaatctgg cctacgatgc tttgagtcag gtccagcgtc cccattattt cgccatcatc
     1201 gacgaagtgg acagcatctt gatagacgaa gcccggactc cgctcatcat cgccgctccg
     1261 gatcaatctt cgagcgatta ttaccgtgtt ttttccaaag cggtgaagca gcttgtcgaa
     1321 ggagaagact acacggccga cgacaagacc aagtcggtcg agatcacgga taagggcatc
     1381 gacaaggtgg aaaagctcgt tggcgtccac gatctttacg aaccgcagaa tttcaggctg
     1441 gtccattatc tcgaagcctg cctcaaagcc cagaggcatt ttctgctcga tcgggattac
     1501 gtcgtccggg acggagaagt ggtcatcgtt gatcaattta ccggacggtt gatgttcgga
     1561 cgccgttatt cggccgggct tcatcaggcg ctcgaagcca aagaaggact gaaagtggcc
     1621 gacgaatcca aaacctacgc ccaaatcacc atccagaatt atttccgtct ttaccaaaag
     1681 atttccggaa tgaccggaac ggcccagact tcggccgagg agtttttcaa agtatataag
     1741 ttggaaacgg tgagcatccc gacgaacaaa cccatgatcc gtaaagatct gccggatttg
     1801 atttacaaga caaaagaagc caaataccaa gcgattgtag aagatataag aacccgccgc
     1861 gaaaaaggcc aaccgatcct gatcggcacc atttccatcg aaaaaaacga ggagttctcg
     1921 aaatatctcg agcgggccgg gatcggtcac gagatactga acgccaaaaa caacgaacgt
     1981 gaaggcgcga ttatcgccca ggccggcaga cccggagcgg tgactctcgc caccaatatg
     2041 gccggccggg gagtggacat tgtcttggga ggcaatccac cggacaagga agcggccgag
     2101 aaagtgagac aggccggcgg tcttcacgtc atcggcaccg aacgccacga agcccggcgc
     2161 atcgacaacc aattgcgcgg acggagcggt cggcagggag acccggggtc ttccagattt
     2221 ttcctttctt tggaagacga cttgatgcgt attttcgggg gagaccgcct gaaatcgatg
     2281 atgcagactt tgaaaatccc cgaagaccag ccgatcgaag ctgggcttgt ctccaaggcc
     2341 atcgccgaag cccagagtaa agtcgaggga ttcaatttcg acatcagaaa gaacctgctc
     2401 gattacgacg atgttttgaa caaacaaaga accaatatct atagccaccg gcaggagatt
     2461 ctcgaatcga acgatgaagg aacgaggaag ctgatcgctt cggctttcga ggaagccggc
     2521 gcggaattgc cgccggattt cgagaaccag ctccaatcat tggatcatct taaagagatc
     2581 cggcgggtga ttctcatgat tctcgacatg ttttggatga accatctcga agacatagaa
     2641 tctttgaccg aatccgtccg catccgcgcc tacgggcagc gcgacccctt ggtcgaatac
     2701 cgtagagaca gccacatgat gtttaacgct atggtccgca atttcaacca atggatcgcg
     2761 gacaatatct tcaagattaa attcgaggag gctccacttt cggaaccggc ttcgccgcaa
     2821 ccggtccggt tttccggcaa gaaagtcggc cggaacgatc cttgtccttg taaatctgga
     2881 aaaaaattca agcattgttg cggaaaataa aatcatgaaa tcttccttca agccaaaacc
     2941 gggacaagtc gattatactc atatacgctg ggctccggtg ataaattgcg tcttgaagca
     3001 taagaataaa atattggtgg ttcgacgcag cgagaaactc aacttttatc ccggttattg
     3061 gaacggcgtt tcgggatttc tcgacgatca aaagagtttg gaagagaaag ttgtcgctga
     3121 attgaaagaa gagctcggta tagcaaaaac ccaagtcgaa aatatccgac ctggagggat
     3181 tttcgatcaa gatgaaccaa agtataagaa aacctggatt gtccatccgg ttctggtgga
     3241 cgtgaaaacc gacaaaatca agctggattg ggaggcggag aattacaaat ggcttacgct
     3301 tcaagaagtg aagaagttga agctcctgcc tgggtttgac gaagttttga gacgattgtc
     3361 tccttggata aaaaataaat gaaatacaat aaactggtgc gggacaagat cccggaaatt
     3421 attaaggaga gcggcgggac accgatcacg catatcgccg ccgacgtcga atattggcta
     3481 aagctcaaag aaaaactgct cgaggaaata aaggagtttt tcgaaaacga gtcgatcgaa
     3541 gagttggccg acgtcaagga agtcttgaac gccattcgcg attacaaagg tttttccgaa
     3601 agcgagctcg aagcaacgag agtcgccaaa gccgaaaaac ggggagcgtt cgaaaagaga
     3661 atcattctcg aagaatcctg atcgacgcca tggaaaaagg gggaatgaaa gagttttacg
     3721 ccggcggatt tttctacaat ccccggacaa aatccgtttt gctccacaaa cggagcccgg
     3781 atgcggaatt caatccggac aagtgggctt ttttcggagg actcagtgaa aacgaagaaa
     3841 cgccgatcga gacctggaga agagaaataa aagaagagct tgatgtcgat cttaccgaaa
     3901 aacaaataaa acctctgagg gattattaca ataaagaccg acagcagcac cgttacgcgt
     3961 ttttcgccga aaccgatctc ccgaaatccg agatgacgct cggcgaaggg gcggatttcg
     4021 attggatacc gcttgatgag gttttcggtt atgatttaac cgcgaagacc gccgaggatt
     4081 tgagattttt catcgaaaat ttcctcgaat aaaaccaatg gacaataatt tcaaaaaatc
     4141 tcccgttgtc aaagacgtca aaaaccccgg aggggaattc gaggaaaaga ctttcgacgg
     4201 gctttttaaa ggccgcgggg tttcgtcttt ttcccgaaaa cgaggtagga tttctccgaa
     4261 aacaatattt tggggagtct ttcttctgac tgtggccttg ggaatcggat cgttcgtcca
     4321 ttatcggaac cagaccatgg aatccgtggc cgagctccaa aaaagcttta tgaaaat
//
LOCUS       DAOPQT010000036         4343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_21999_length_4343_cov_3.475746, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000036 DAOPQT010000000
VERSION     DAOPQT010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21999_length_4343_cov_3.475746"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKG74_01395"
     CDS             complement(<1..456)
                     /locus_tag="PKG74_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOB89971.1"
                     /translation="MMKPKSRTKKNKTRFVFVTGGVISGIGKGIVASSLGRLLISCGY
                     SIRQIKIDPYLNEDAGTMNPFQHGEVFVTDDGAETDLDLGHYERFTSVPLTKKSNFTS
                     GSVFRSVLDKERNGSFLGKTIQFVPHITDEIKRRILEMAEAEKPDFLIVE"
     gene            complement(560..630)
                     /locus_tag="PKG74_01400"
     tRNA            complement(560..630)
                     /locus_tag="PKG74_01400"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(596..598),aa:Gly,seq:ccc)
     gene            complement(631..906)
                     /locus_tag="PKG74_01405"
     CDS             complement(631..906)
                     /locus_tag="PKG74_01405"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOB89972.1"
                     /translation="MWKIAITVLLAALIFAAGFELYGIYQAYVGVKNNYGELKTKAEA
                     LREENEKLEAEVSYLSIPENLLKALRERFNYKFPGEKSIIVVPKQGE"
     gene            complement(930..2684)
                     /gene="lepA"
                     /locus_tag="PKG74_01410"
     CDS             complement(930..2684)
                     /gene="lepA"
                     /locus_tag="PKG74_01410"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOB89973.1"
                     /translation="MENIRNFVIIAHIDHGKSTLADRMLEITGAVPKRLMRPQYLDQM
                     DLERERGITIKMTPVRMIYRPEPDQEYVLNLIDTPGHSDFGYEVSRALKAVEGAVLLV
                     DATQGIQAQTLANFETARKAGLKIIGCLNKIDLNPPDLEKKVKELANLLNEPTERIYR
                     VSGKTGEGVAELLKGIAKEVPPPRSDRGKQALIFDSVYNDHKGIIAYVRVFSGEFSAK
                     EESSLMAADRRFNIKEVGIFSPEFKPTEKLSSGEIGYIATGIKDPGVLRIGDTIGDRP
                     LEGYNPPKPVVFVSLYPDEGDKYDDLKAALNKLRLVDSSISVEPDYSEILGRGFKAGF
                     LGRLHFEIATERLNREFDLPVIASFPSVAYRVKTKSGVKLVESPKDFPDDNFGAEEPV
                     TSLKIIVPQIYLGRILGLKDIFRMEIVGTEVIDDRIVIYATMPLSELISDFDDRLKSV
                     SLGYASFSYEISGWQEAEVAKMEVLVANEIVPGLTRIVYKDELDHVARQTVEELKNLL
                     PKQLFAQPIQAAVGSRIVARETIPALQKKLGYFGKTGGDRSRKMKLWKKQQAGKKRLQ
                     ASGRVTISPEIFKELLKK"
     gene            complement(2684..>4343)
                     /locus_tag="PKG74_01415"
     CDS             complement(2684..>4343)
                     /locus_tag="PKG74_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HOB89974.1"
                     /translation="RNISSGAEPLDWTLSGNPLIFPGIDSLEEGQHFPEYLVAFNKDI
                     DAAETEMSDLRQNGLEWLFEDGEALMRSLDRLENTITSINNKAAALRNIVSKLGFAGR
                     ISPVYLNLQSGLYTIKDGISGLKETLSGNGHLVFVFENVSEIRPAGGFNGSYADVSFN
                     NGKIENLEVNDIFYPDQHFEEKIVPPKPLQVIAKDWGARDANWFFDFPSSAEKTISFL
                     SESSIYKDAGVEFTGVVAVNSRAVERILEICGPIELPEYKLVLTKDNFLNEIQEEVSL
                     NSASKGSARKQILKVLAPEILERIRQFDEAEKEKLFTSVIESLNRKDIRVHFKNKKLA
                     TVAEALSATGSVYKFSEGDYADYLAVANANINGGKTDIFMKQEISLKSELSADGTIKN
                     NLEIVRTHTGQNEKLSFYKAINQDYIQILAPRDAKALKVAGNTSRTVSAKVDYVKSGY
                     AKDPDVEIFESGFQSGKKVLGYWLDVKPGKTGKLSVSYEQPSMVRPRFRFVYEKQSGV
                     ESALKYEVSAPPGYVFKEIGGSVFTYESADPPKRLIIDLNLEHI"
BASE COUNT          977 a         1131 c         1078 g         1157 t
ORIGIN      
        1 ttcgacgatc aaaaagtccg gcttctccgc ttccgccatt tcgaggatgc gccgcttgat
       61 ctcgtcggtg atatgcggaa cgaactgaat ggtcttaccg agaaaagaac cgtttctttc
      121 cttatcgagc acggaccgga aaaccgaacc gctcgtgaaa ttggattttt tggttaaagg
      181 caccgaggta aacctttcgt aatgtccgag atcgagatca gtttcggcgc cgtcatcggt
      241 gacaaaaacc tctccgtgct ggaaaggatt catggtgccg gcgtcttcgt tcagataggg
      301 atcgatcttg atctgcctga tggagtagcc gcaggaaatc aaaagccgac cgaggctcga
      361 agcgacgatg cctttcccaa tgccggagat gactcctccg gtgacaaaaa cgaacctggt
      421 tttgttcttt ttcgttcggg atttgggttt catcattcgt cgctgattcc tcggatatgt
      481 aaaactttct ccattataca cccggcttta aaccgggcaa acaagataag tgtggttaac
      541 tccaaaagcg ttttatatga gcgggatacg ggaatcgaac ccgcgtctca accttgggaa
      601 ggtcgtatat ttccactata ctaatcccgc ctactcaccc tgtttgggaa cgacgatgat
      661 ggacttttct ccggggaact tgtaattgaa tctctcccgc aaagccttca aaaggttctc
      721 cggaatgctc aaataactca cctccgcctc cagtttttcg ttttcttccc ggagcgcttc
      781 ggctttggtc ttcaattcgc cgtaattgtt cttgaccccg acataagcct gataaatgcc
      841 gtagagctca aaacccgcgg cgaaaataag cgcggccaaa agcacggtga tcgcgatttt
      901 ccacatctcg atcgtatttt aaccgagaat tattttttca acaattcctt gaaaatctcc
      961 ggcgagatgg tcacccggcc cgatgcctgg agcctctttt ttccggcctg ctgttttttc
     1021 caaagcttca ttttgcggct gcggtctccg ccggtttttc cgaaataacc aagcttcttt
     1081 tgtaaagccg gaatggtttc gcgggccacg attctgctcc cgaccgccgc ctggatcggt
     1141 tgggcaaaaa gctgtttcgg caggagattc ttcaattctt cgacggtttg tctcgccaca
     1201 tggtcgagtt cgtctttgta gacgatcctt gtgagccccg ggacgatttc gttcgccacc
     1261 aaaacctcca ttttggccac ttcggcttct tgccagccgg agatttcgta gctgaaagaa
     1321 gcatatccca aagacaccga tttcaagcgg tcgtcaaagt cggagatcag ttcggataac
     1381 ggcatggtgg cgtaaatgac gatccggtcg tcgatcacct cggtgccgac gatctccatc
     1441 cggaaaatgt ctttcaatcc caagatccgg ccgaggtaaa tctggggaac gatgatcttt
     1501 agactggtca ccggttcttc cgccccgaaa ttatcatccg gaaaatcctt cgggctttcc
     1561 acgagcttga cgccggattt ggttttgacg cggtaagcga ccgatggaaa actggcgatg
     1621 accggaagat cgaattcgcg gttcagacgt tcggtcgcga tttcgaaatg gagtcttccc
     1681 aagaatccgg ctttgaatcc ccgacctaag atctcgctgt aatccggttc gaccgatatc
     1741 gaagaatcga ccaggcgcaa tttgttcagg gcggctttca ggtcatcgta tttgtctccc
     1801 tcgtccggat acaaagaaac gaagacaacg ggcttcggag ggttgtagcc ttcaagcggt
     1861 cgatcgccga tggtgtctcc gattctaagc acgcccgggt ctttgatgcc ggtggcgata
     1921 tagcctattt ctccggaaga aagtttttcg gtcggcttga attccggaga aaagatgccg
     1981 acctctttga tgttgaatcg cctgtcggcg gccataaggc tcgattcttc tttggccgag
     2041 aactcgccgg aaaaaacccg gacataggcg atgatgcctt tgtggtcgtt gtaaaccgaa
     2101 tcgaaaatca aagcttgctt gccgcggtcg cttcgcggcg gaggcacttc tttggcgatt
     2161 cctttcaaaa gttcggccac gccttcgccg gtttttccgg aaacacggta aatcctttcc
     2221 gtaggctcgt tcaaaagatt ggccagttct ttcacctttt tttccaaatc cggcggattc
     2281 aagtcgatct tgttgaggca gccgatgatt ttaagtccgg ctttgcgggc cgtctcgaaa
     2341 ttggccaagg tctgcgcctg gatgccctgg gtggcgtcga ccaggagcac cgcgccttct
     2401 accgctttca gggcgcgcga tacttcgtag ccgaaatcgc tgtgcccggg cgtgtcgatc
     2461 aagttcaaga catactcttg gtcaggctcc ggacgataaa tcattcggac cggcgtcatt
     2521 ttgatggtga tgccccgctc cctttccaaa tccatctgat cgagatactg gggacgcatt
     2581 agtcttttgg gcacggctcc ggtgatctcg agcatgcggt cggccaaggt tgattttccg
     2641 tgatcgatgt gggcgataat gacgaaattg cggatgtttt ccatcaaatg tgttccaggt
     2701 tcagatcgat aatcagacgc ttcggcggat cagccgattc gtaagtaaaa acagaacctc
     2761 caatttcttt gaaaacatat cccggcggag ccgaaacctc gtatttcaag gccgattcga
     2821 ctccggattg cttttcgtag acaaagcgga aacggggcct taccattgac ggctgttcgt
     2881 aggaaacgct caatttgccg gttttgccgg gtttcacgtc cagccaatat cccaagactt
     2941 ttttgccgga ttggaacccg gattcgaaaa tctccacgtc cggatccttg gcgtaaccgg
     3001 atttcacgta atccactttg gcgctaaccg tccgtgacgt gtttccggcg actttcaagg
     3061 ctttggcatc tcgcggagca aggatctgga tataatcctg gttgatcgcc ttgtaaaaac
     3121 tcaatttctc gttttgcccg gtatgggtcc ttacgatctc cagattgttt ttgatcgtgc
     3181 catcggccga aagctcgctt ttcagggaaa tctcctgctt catgaaaata tccgtcttcc
     3241 cgccattaat gttggcgttc gccaccgcca gataatcggc gtaatcccct tcggaaaact
     3301 tgtagaccga tccggtggcg ctcaaagctt cggccacggt cgcgagcttc ttgttcttga
     3361 aatgaacgcg gatgtctttc cgattcaagg attcgatgac ggaagtgaaa agcttttctt
     3421 tctcggcctc gtcgaattgt cttattcttt ccaagatttc cggggccaag actttcagga
     3481 tctgcttgcg ggccgatccc ttggaagcgg aattcaaaga aacttcttcc tggatctcgt
     3541 tcaggaaatt atccttggtc aaaacgagtt tgtattccgg aagctcgatc ggaccgcaga
     3601 tttcaagaat ccgctcgacc gctcggctgt ttacagcgac gacgccggtg aattccacgc
     3661 ccgcgtcctt ataaatcgag gattcgctca aaaaagaaat cgttttctcg gcggacgacg
     3721 ggaagtcgaa aaaccagttg gcgtccctgg ctccccagtc tttggcgatc acctgcaaag
     3781 gcttcggtgg aacgattttc tcctcgaaat gctggtccgg ataaaaaata tcgttcacct
     3841 ccagattttc tatttttccg ttgttaaaag aaacatcggc gtagctgccg ttgaagcctc
     3901 cggccggcct gatttcggaa acgttttcga aaacgaaaac caaatgcccg tttccagaga
     3961 gggtttcctt gagcccggaa atcccgtctt taatagtgta aagcccggat tggagattca
     4021 gataaaccgg tgagattctt ccggcaaagc ccagtttgga aacgatgttc cgcaacgcgg
     4081 ccgctttgtt gttgatcgaa gtaatggtgt tctccaaccg gtcgaggctt cgcataagcg
     4141 cttctccgtc ttcgaaaagc cattcaaggc cgttctgtcg caagtcgctc atttccgttt
     4201 ccgcggcatc gatatcttta ttgaaagcca ccagatactc gggaaaatgc tggccttctt
     4261 cgagagaatc gattcccgga aagatcaaag gattcccaga taaagtccaa tcgagcggtt
     4321 cggctcctga agaaatattc cgg
//
LOCUS       DAOPQT010000037         4332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_22069_length_4332_cov_2.905775, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000037 DAOPQT010000000
VERSION     DAOPQT010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22069_length_4332_cov_2.905775"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PKG74_01420"
     CDS             <1..738
                     /locus_tag="PKG74_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89975.1"
                     /translation="TQIRIGNDYSTSVGTLYSGANGFLASNSYQTTLGVNSWSKNAPT
                     YASTIAILGINATLTQPAFQIKYSPGNTPTGAFSSFFTNDLLTVLGNGNVGIGTTSPQ
                     AKLQIVSTDSDNTVLSFYDSTRNQGLGILVNKAPTGSWPPVVENTTMTLQSSGGSAGN
                     LAFATGNTERMRIAAGGNVGIGTISPSSKLTVEGTFSVSGNANICNLVSYTGGGGTTQ
                     CPAGFYTWSGVALTSGYMLCCKVSNPI"
     gene            844..2346
                     /locus_tag="PKG74_01425"
     CDS             844..2346
                     /locus_tag="PKG74_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89976.1"
                     /translation="MTIFINRLKNNPKLRGVKCFVLIALVAAVTAVALNNGNPTSSVK
                     TGENKNLVLPEGKQAYKISQAAEAWPKFIEATIDPVDVHVGDTQRLSVSVDDDVNIVS
                     VEAHIETDNDVVILPLKFAGSESEEIIEKKNIFEDLFSRFRSVPKQPGVSSYEGDWIV
                     KDTHNDTYHTVFIAKDAAGRENTIILAWSDACGIPVGGNFTLSSNCVISSNDGVDNGN
                     LIINPGVTLTLNSTFAFNSGKTITVNGAIAKGAGGILKKTNIWMVDVDNDNYPASSTQ
                     YLQDTAPTNGKRRNTLNAGGIDCNDNSASVYPGTVTTGSCSCNYSSTCSESASGTTTT
                     STCSSDGTFTGGSGSCNCSRDTDGNSCGSGDYCSGGSCVEAEEPCGGGCGISWYCNGC
                     THMAQNYNCVDDECVAGTLLNYGCSTGFCGCECCSSSDCGSGETCSGNNCICSARGSW
                     SCLCNDGYEPSGSLGAGVECRYGGGPETCLSRCSSHGGWSGSWSCEDAPC"
     gene            complement(2421..2495)
                     /locus_tag="PKG74_01430"
     tRNA            complement(2421..2495)
                     /locus_tag="PKG74_01430"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2461..2463),aa:Ile,seq:tat)
     gene            complement(2515..4326)
                     /gene="ftsH"
                     /locus_tag="PKG74_01435"
     CDS             complement(2515..4326)
                     /gene="ftsH"
                     /locus_tag="PKG74_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOB89977.1"
                     /translation="MIVLTAVFLVLVSVLSFSESREKPEPISLNNLAQKIVAGEISEV
                     VVDYNVLEVLSRDAKTFVASKEADADFVSLMRNYGVPAEALQAVDLEVKDNTTKELVI
                     GAITTIILPLLLLAGLMFMLTRRAVGNANQAFSFGRANIHLFTNFKDRITFKDVAGAK
                     EAKQELEEVVDFLKNPKKFLDLGAKIPRGVLLMGPPGSGKTLLARAVAGEAGVPFFHI
                     SASEFVEMFVGVGASRTRDAFQTAKKASPSILFIDEIDAVGRERGAGLGGGHDEREQT
                     LNQILVEMDGFNRDTNVIVLAATNRPDILDSALLRPGRFDRRIVLDLPDISEREEILR
                     LHAKEKPVGPDIDFRKIAVRTPGFSGADLANVMNEAAILAARNNKKTIDQQNVYDSIE
                     KVLLGPERRSKIVSDKEKRVTAFHEAGHALVSVGVKNSDPVHKVSIVSRGRAGGYTLK
                     LPTEDQRLKTKEQFLADLAVLLAGFVAEKQVFGDVSTGASSDLKTASEISRKLVTRFG
                     MSDKLGPIVFGDTEELIFLGREISAEKNYSEKVAALIDDEVKEFVLRAKKAAEKILKT
                     NTKALTKIAEALLKEETIEQEAFYRLVKPFNLKPISV"
BASE COUNT         1026 a         1094 c         1134 g         1078 t
ORIGIN      
        1 actcagatac gtatcggtaa tgattattcg acatcggtag ggacactcta ttccggagcg
       61 aacggctttt tggcgagcaa tagctatcaa accactttgg gcgtcaattc ctggtcaaag
      121 aatgctccta cctatgcatc gaccattgct attttgggga ttaatgccac cctaacacaa
      181 ccggcttttc agataaaata ttctccgggc aatacgccca ccggcgcatt cagttcgttt
      241 ttcaccaatg atttgttgac tgttttggga aacggcaacg tcggtattgg aaccacgagt
      301 ccgcaagcca aactccaaat tgtatccacc gatagcgaca acaccgtttt atctttttat
      361 gattccacca ggaatcaagg tctgggtatt cttgttaata aagcgcctac gggttcgtgg
      421 ccgccggtgg tggagaatac gaccatgact ttacaatctt ccggaggctc ggccggcaac
      481 ctggcttttg cgaccggcaa taccgaaaga atgaggatcg ccgccggagg caatgtcgga
      541 atcggcacga tttctccttc ttccaaactg actgttgagg ggactttttc cgtctccggc
      601 aacgccaata tctgtaatct agtttcctat accggaggcg gcggcaccac ccaatgtccc
      661 gctggctttt atacctggtc tggtgtcgct ttaacttccg gatacatgct ctgctgtaag
      721 gtgagtaatc cgatctaatg taaatcaaga cagtcccgcc gaaatcggcg ggctgttttg
      781 tataatcata agactttgtc gaaatgctat aataaaacca tcggaaaaaa gttttctaaa
      841 accatgacta ttttcatcaa tcgtttgaaa aataacccaa agcttcgggg ggttaagtgt
      901 tttgttttaa tcgctctcgt cgcggcggtg acagcggtcg ctttaaataa cggaaatccg
      961 acttcgtcag tcaaaaccgg agaaaataaa aatcttgttc tccccgaagg aaaacaagct
     1021 tataagatca gccaggcggc cgaggcctgg ccgaagttta tcgaggcgac aatcgatccg
     1081 gtcgacgtcc acgtcgggga tacccaaagg ttgtccgtct ctgtcgacga cgacgtgaat
     1141 atcgtctccg tcgaagccca tatcgagacc gacaatgatg tcgttattct gcctttgaag
     1201 ttcgcggggt cggaatcgga agaaattatc gagaagaaaa atatattcga agatcttttt
     1261 tcgcgatttc gatcggttcc aaaacagcct ggcgtttcct catacgaagg agactggatc
     1321 gtcaaagaca cccataacga tacttaccac acggttttca tcgccaaaga cgcggccggc
     1381 cgggaaaaca caatcatttt ggcttggagc gatgcctgcg gcattcccgt gggcggaaac
     1441 ttcacgttaa gttcgaactg cgtcatttct tcaaatgacg gagtggacaa cggtaatttg
     1501 attatcaatc caggcgtcac cttgacgttg aattccacct tcgccttcaa ttccggtaaa
     1561 acaatcaccg tgaacggagc gatcgccaaa ggagcagggg gtattttgaa aaaaaccaac
     1621 atttggatgg tagacgttga caatgataat tatccggcaa gctccaccca atatcttcaa
     1681 gatacggccc cgaccaacgg aaaacgcaga aatactttga atgccggcgg tatagattgc
     1741 aacgacaata gcgcgagcgt ttatccggga acggtcacga ccggaagctg ctcttgcaat
     1801 tattcttcca cttgttccga atcggcgagc ggcaccacga caacaagcac ttgcagcagc
     1861 gacggaactt tcaccggagg atccggatcg tgcaactgct cgagagacac cgatggcaat
     1921 tcttgcggat ccggagatta ttgtagtggc ggaagttgcg tagaagccga agagccgtgt
     1981 ggtggaggtt gcggtataag ttggtattgc aacggatgta cccacatggc ccagaattat
     2041 aattgcgtcg acgacgaatg tgtcgctggg accctactca attacggttg cagtaccggt
     2101 ttttgcggat gcgagtgttg ttccagctcc gattgcgggt ccggcgagac ttgttccggc
     2161 aataactgta tctgttccgc gcggggaagc tggagctgtc tttgcaacga cggttacgag
     2221 ccgtcgggaa gcttgggagc cggagtggaa tgtcgatacg gtggaggccc cgaaacatgc
     2281 cttagcagat gttcgtctca cggcggctgg agcggatcct ggagttgcga agacgcgcct
     2341 tgctgatttt gtcctttcgt gaatacgaat cagggtgagc ggcgatccga tttctaaaac
     2401 ccccgcctta ggcgggggaa tggtcggcgc ggcagggatc ggacctgcga cccatgcttt
     2461 ataagagcat tgctctacca ctgagctacg cgccgtctct cagccaagct taatctaaac
     2521 cgatatcggt ttcaaattaa agggctttac caggcgatag aatgcttcct gctcgatggt
     2581 ctcttccttg agtaaagctt cggcgatttt agtcagggcc tttgtatttg ttttcaatat
     2641 tttctcggcc gcttttttcg ctctcaagac gaactctttg acttcgtcgt cgatcaaggc
     2701 ggccactttt tccgaatagt ttttctccgc ggagatctcc cgtcccaaaa agatcagttc
     2761 ttcggtgtcc ccgaaaacaa tcggccccaa tttgtcgctc atgccgaagc gggtgacgag
     2821 cttgcggctg atttcggaag cggttttcaa atcgctcgag gcgccggtcg aaacgtctcc
     2881 gaagacttgt ttttcggcga caaatccggc aagaagcacg gccaaatcgg ccaagaactg
     2941 ttctttggtt ttgagtcttt ggtcttcggt cgggagtttc aaagtatagc ctccggcgcg
     3001 gccgcggctc acgatagaaa ccttatgaac cggatcggaa ttcttgacgc ccacggaaac
     3061 gagcgcgtgt ccggcttcgt ggaaagcggt cacgcgtttt tccttgtcgg agacgatctt
     3121 actccggcgt tcgggtccga gcaggacttt ctcgatggaa tcgtaaacgt tttgttggtc
     3181 gattgttttt ttgttgttac gggccgcgag aatggccgct tcgttcatga cgttcgccaa
     3241 atcggctccg gaaaatcccg gagtgcgcac cgctattttc cggaaatcaa tgtccggacc
     3301 aacgggtttt tctttggcgt gaagccgcag gatctcttcc cgttcggaaa tgtccggcag
     3361 atcgagcacg atccgacgat cgaatcttcc cggccgcaat aacgccgagt cgagaatatc
     3421 cggtcggttg gtggccgcga gcacgatgac gttcgtgtcc ctgttgaatc cgtccatttc
     3481 gaccaaaatc tggttcaggg tttgttcgcg ttcgtcgtgt cctccgccca agcccgcgcc
     3541 gcgttcacgt ccgacggcgt cgatctcgtc gatgaaaaga atcgagggag aagctttttt
     3601 ggctgtttgg aaagcgtcgc gggtgcgcga agcgcccacg ccgacgaaca tttccacgaa
     3661 ttccgaagcg gatatatgga aaaaaggaac cccggcttcg ccggcgaccg ctctcgcgag
     3721 caaggtcttt cccgatcccg gaggccccat aagaagaacg cccctcggaa tcttcgctcc
     3781 caaatccagg aattttttag ggtttttcaa aaaatcaacc acttcctcga gctcttgttt
     3841 ggcttctttg gcaccagcca cgtctttaaa agtgatccgg tccttgaaat tggtgaacaa
     3901 atggatattc gcccgcccga aagaaaaagc ctggttggcg tttccgacgg ctcgcctcgt
     3961 cagcatgaac atgagtccgg cgaggagaag caggggaagg atgatggtcg tgatcgctcc
     4021 gatcacgagt tccttggtgg tgttgtcttt gacctccaga tcaacggcct ggagcgcttc
     4081 ggccggaact ccgtagttcc gcatcagact gacgaaatcg gcatcagcct ctttggaggc
     4141 gacgaatgtt ttcgcgtcgc gcgagagaac ttccaaaaca ttgtaatcga caaccacttc
     4201 ggagatttct ccggcaacga ttttctgggc caggttgttt agggaaatcg gttcgggctt
     4261 ttcgcgggat tcggagaaag agaggacact gacgagaaca aggaaaacgg cggttaaaac
     4321 aatcaagctt tt
//
LOCUS       DAOPQT010000038         4229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_22731_length_4229_cov_3.786775, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000038 DAOPQT010000000
VERSION     DAOPQT010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22731_length_4229_cov_3.786775"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="PKG74_01440"
     CDS             <1..1781
                     /locus_tag="PKG74_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOB89978.1"
                     /translation="RLLNNLDKLDWSEKVKIAQKNWIGRSEGALIKFGVSGLKSPIVV
                     FTTRPDTILGATYLVLAPESEIIQSLQKKIKNWGEVEKYVAKAAGKSEAERISEEREK
                     TGVELKGVKAINPGNNMEIPIWVSDYVVQGYGTGAIMAVPAYDERDFAFSERFGLPVV
                     KAPLIPESQAIRQTKAKRTTEYRLRDWLISRQRYWGPPIPLIYCEHCAEALKKIGPKR
                     AKTKGWSRGEIENPGWIPVKEKDLPVVLPFVKDFRPTGSGSSPLASVKSFYETKCPRC
                     GRIGHRETDVSDTFLDSAWYFLRYPSVNDKKRAFDSKLTKKWLPVDMYLGGAEHAVLH
                     LLYSRFLTMALKDLGHIDFEEPFSVFHAHGLLLRNGAKISKSKGNVINPDDYIKRFGA
                     DAFRMHLMFLGPLEEGGDFRDAGIVGITRFLDRVWNLVVHFKPRGRKDLSPWLHASVK
                     KITKSVSDLRYNVAIAELMIIMNKFSERPDEVSKKDLETFILMLAPFAPFLSEEAWLM
                     IGNKPSIHSKPWPSYEESGIESGSFELVIQVNGRVRDRVEVESGIEAKEAEKIALGRP
                     KVRAFLKGSKPIRVIFVEGRLINIVV"
     gene            1900..4158
                     /locus_tag="PKG74_01445"
     CDS             1900..4158
                     /locus_tag="PKG74_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOB89979.1"
                     /translation="MTITINRKVLIAIIIALILVGIFYLANRANLIFRRFSATILSSL
                     EGGVVTVPQKISNLVAVSGDEQVSLAWAAPIDGGSKITAYRIYRGESSNNLDFLTAIS
                     RESFTDSAVKNGTTYYYKVAAVNIMGEGPMSDAVNALPSISGSQPISKNDNGGAVVAP
                     AVSVPSAINSLVAVSGNNLVSLAWSAPYNGGRPISLYKIYRGTWAGGETFLTSTNSSA
                     FTDTNLSNNTAYYYRVSAVNSVGEGSLSNRVSANPTAYNYVYNAGLPGQISNLTAIPG
                     DGQVSLSWSATSGSAITGYKIYRRDYYGNSAYVSTVSTNSFVNYGLSNGQTYYFRVSA
                     VNSTGEGPMSAEVGATPVASSYYYYNYNQIPGQVTGLSANPGNNQVYLSWSTPSSGST
                     SVSYYKIYRSTDYYGDSFYTTAGSTSFIDASVSNGTTYYYRVSAVNSVGEGSLSNRVS
                     ATPYSTYVYYPPSNPKTAPGQITNLTAQNSSGYALLSWSTPYNGGSSIQNYKIYRGTS
                     SGNLSLLATTQFTNYTDSSVSSGKNYYYKVSAVNSYGEGILSNQASVKIPSQTYYYDD
                     DDDDDYVYVPSYSSEYVRITSLPSSVTEGQTFSVSWQSSSHFQGCRVWFQGSNITKDS
                     LPTNGSASFSTSGIPAGTYEVGVRCFRPYEIDSMSNYKEDDYMTNSAYNSIGKRDGKN
                     IVVIKAYGQTSTSSINGSYSCVCNDGTVLGGALSSGVLCSNVGGVETCVSKCLPYGGW
                     KGGTSGFTCVNY"
BASE COUNT         1066 a         1039 c         1062 g         1062 t
ORIGIN      
        1 agaggttgtt gaacaatctc gataaactgg attggtcgga aaaggtgaaa atcgcccaaa
       61 agaactggat cggccgctcc gaaggagctc tgatcaaatt cggcgtttcc gggctgaagt
      121 cgccgatagt tgttttcacg acaaggccgg acaccatcct cggcgcgact tatctggtac
      181 tcgctccgga gagcgagatc atccagtcgt tacaaaagaa gatcaaaaac tggggagaag
      241 tcgaaaaata cgttgccaaa gccgccggaa aatccgaagc cgaaagaatc agcgaggaaa
      301 gggaaaaaac cggcgtcgaa ctcaaaggcg tgaaagcgat caatccggga aacaacatgg
      361 aaatcccgat ttgggtttct gattacgtgg ttcagggcta cggcacggga gcgatcatgg
      421 cggtgccggc ttacgacgaa cgcgactttg ctttttccga aagattcgga ttgccggtcg
      481 tgaaagctcc tttaattccg gaaagtcagg cgatcagaca aaccaaagcc aaaagaacaa
      541 ccgaataccg gcttcgcgac tggcttattt cgagacagcg ttattggggt ccgcccattc
      601 ctttgattta ttgcgaacat tgcgccgagg ctttgaagaa gatcggtccg aagcgagcca
      661 aaaccaaagg atggagtagg ggagaaatcg agaatcccgg ttggattccg gtgaaggaaa
      721 aagatctgcc ggttgttttg ccttttgtga aggatttccg gccgaccgga tccggatcct
      781 cgcctttggc ctcggtcaaa agcttttacg aaaccaaatg tccgcgttgc ggccggatcg
      841 ggcacaggga aacagatgtt tcggatactt ttctcgattc ggcttggtat tttttgcgtt
      901 atccttcggt gaacgacaaa aaacgcgctt tcgattccaa gctaaccaaa aaatggctgc
      961 cggtcgacat gtatttggga ggcgccgaac acgccgtgct ccatctttta tattcgagat
     1021 ttctgaccat ggctttgaaa gacctcgggc acattgattt cgaagagcca ttttccgttt
     1081 tccacgccca tggattgctt ttacgaaacg gagcgaagat ctccaaatcc aaaggcaatg
     1141 tcatcaatcc ggacgattac atcaaaagat tcggcgccga cgctttcaga atgcatttga
     1201 tgtttttggg tccgctcgag gaaggagggg atttccgcga tgcgggtatt gtcggcatca
     1261 ccaggttttt ggaccgggtc tggaatctcg tcgttcattt caaaccgcgc gggagaaaag
     1321 acctttctcc atggctccac gcttccgtga agaaaatcac caagagtgtt tccgatctcc
     1381 gctacaacgt cgccatcgcc gaattgatga ttatcatgaa caagttttcc gagcggccgg
     1441 acgaagtgag taaaaaagat ttggaaacat ttatcctaat gctcgcgcct ttcgctccgt
     1501 ttttgagcga agaggcgtgg ctgatgatcg gaaacaaacc gagtatccat tccaaaccct
     1561 ggccttccta cgaagaaagc ggtatcgagt ccggaagttt cgagttggtg attcaggtga
     1621 acggccgggt gagggatagg gtggaggtgg aatccggaat agaggccaaa gaagcggaaa
     1681 aaatcgcttt gggaaggccg aaagtcaggg cctttctcaa aggatcaaaa ccgatcaggg
     1741 tgattttcgt cgaaggacgt ttgatcaaca tcgtagtctg aaatcaagaa cataagccga
     1801 aaaagccgcc ttttgcttat aaaaagcggc tttcttgact aaaatgccaa aatgttatat
     1861 aattaatata ttgagtcaaa aatccaataa accatagaaa tgactattac catcaataga
     1921 aaggtcttga tcgcgattat tatcgccttg attctggtcg gtatttttta cttggcgaac
     1981 agagccaatc tgattttcag aaggttttcc gccaccattc tttcgagtct cgaaggagga
     2041 gtggtcaccg ttccccagaa gatctccaat ctggtggccg tttccggaga cgaacaagtt
     2101 tcgctcgcct gggccgcgcc gatcgatgga ggcagcaaaa tcaccgctta tcggatttat
     2161 cgcggagagt cttcgaataa tctggatttc ttgaccgcga tttcgcgcga gtctttcacc
     2221 gacagcgcag tcaaaaacgg cacgacttat tactacaagg tggcggccgt gaacatcatg
     2281 ggcgaaggcc cgatgtccga cgcggtgaat gctttgccga gcatttcggg atcccagccg
     2341 atttcgaaga acgacaatgg tggcgctgtt gtcgcgcccg cggtttcggt tccctcggcc
     2401 atcaacagct tggtggcggt ttccggaaac aatctggttt ctttggcttg gtccgctcct
     2461 tataatggcg gccgaccgat cagtctctac aagatttacc gcggcacttg ggccggcgga
     2521 gaaacttttc tcacttccac gaattcttcg gcttttaccg acaccaattt gtcgaataac
     2581 accgcctatt attatcgtgt ctcggccgtg aactccgtgg gtgaaggaag tctatcgaac
     2641 agggtctcgg ccaatccgac ggcctataat tatgtctaca atgccggatt gcccggacag
     2701 atttccaatt tgacggccat tcccggagac ggtcaggttt ctctttcttg gtccgcgaca
     2761 agcggatccg ccatcaccgg atacaagatt tatcggaggg attattacgg caattcggcc
     2821 tatgtctcga ccgtgtccac gaattctttc gtgaactacg gtttgagtaa cggccagacg
     2881 tattacttta gagtctccgc cgttaactcc acgggtgaag gcccgatgtc ggccgaggtc
     2941 ggcgccaccc cggtcgcttc gtcttactat tactacaact acaaccagat tccgggacag
     3001 gttaccggac tttcggcgaa tcccggcaac aaccaggttt atctctcgtg gtcaacgccg
     3061 tcttccggct cgacctcggt ttcctattac aaaatctatc gttcgaccga ttattacggc
     3121 gacagctttt atacgactgc cggatcgacg agttttatcg acgcttcggt ttctaacggc
     3181 acgacttatt attaccgcgt ctcggccgta aactccgtgg gtgaaggaag tctatcgaac
     3241 agggtctcgg caacgcctta ctcgacctac gtctattatc cgccttccaa tccaaagacc
     3301 gctcccggcc aaatcaccaa tttgaccgcc caaaacagct ctggttacgc tcttttgtcc
     3361 tggagcactc catataacgg cggaagctcg atccagaatt acaagattta cagaggcact
     3421 tcttcgggta atctttcctt gcttgcgacc acccaattca ccaactatac cgattcgagt
     3481 gtttcaagtg gtaaaaatta ttactataaa gtttcggccg tgaactctta cggagaaggc
     3541 atcttgtcga atcaggcttc agtgaagatt ccttcccaga cttattatta tgacgacgat
     3601 gatgatgacg attacgtcta tgtgccttcg tattcgagcg aatacgtgag aatcacgagt
     3661 cttccttcgt ccgtaaccga aggtcagact ttctcggtca gctggcagtc ttcgtcccat
     3721 ttccaaggat gccgcgtctg gttccaggga tcgaatatca ccaaagacag tcttccgaca
     3781 aacggcagcg ccagtttttc cacatccggc attccggccg gaacgtacga agtcggcgtc
     3841 aggtgcttca ggccttacga aatcgattcg atgtctaatt ataaagaaga cgactatatg
     3901 accaattcag cgtataacag catcggtaag cgtgacggta aaaatattgt tgttattaaa
     3961 gcttatggtc agacctcgac gagctcgatc aacggttcct atagttgcgt ttgcaacgac
     4021 ggcaccgtat tgggtggagc tttatcttcc ggcgtattgt gttccaatgt tggcggtgta
     4081 gaaacttgcg tcagtaaatg ccttccttat ggcggatgga aaggtggaac cagcgggttc
     4141 acttgtgtga actattaagc cgaaacaaaa aaatctcccc cgtctaagcg ggggagattt
     4201 tgaatccggt gttattccgt tgtcacgcc
//
LOCUS       DAOPQT010000039         4199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_22933_length_4199_cov_3.451979, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000039 DAOPQT010000000
VERSION     DAOPQT010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22933_length_4199_cov_3.451979"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKG74_01450"
     CDS             complement(<1..210)
                     /locus_tag="PKG74_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89980.1"
                     /translation="MFYKERMNLNLNEGDWNYLLNLTTTLINTSEIYLQPSLMYLKAI
                     SEFHLDDLQAAYSTFNELSRITDQAS"
     gene            316..960
                     /locus_tag="PKG74_01455"
     CDS             316..960
                     /locus_tag="PKG74_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89981.1"
                     /translation="MKKVRLWVAAVILIAIGLGIFVYLQPKPIEPVDPVETAEVPEVS
                     DEPIEEIAVADLYEVPEPKEDGTLVGSFGPLFPPGTIYEIVSSETVSKQGSWAQIIRI
                     NLSADETRLLAVSEGTANLTDDKRKDFAALSAIAGTAKLEIQVCFSVGSEILFEDDSF
                     LRRGDTMAIIRGKSTFDPEVTVVIAVSPWNEGDTELVDQFIQLIKLGSQNPEGS"
     gene            1075..3129
                     /locus_tag="PKG74_01460"
     CDS             1075..3129
                     /locus_tag="PKG74_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89982.1"
                     /translation="MIIRATAREMEEDGYDWQFLSPKEKLIQVVNQEVYCPRPSGCVM
                     GECFVTESYDYTAGNCPGQACKDLVNEINAANATNTKNLTTLKGEGLLKSWTKYDLIL
                     YTISNWFFSPDYGSWEWHELKEAYSDADKCTNDSSSNKCLANNVGLWAGSCEGGAGGF
                     FKCCCHNQTNKPISSVPDPAADNHPPLEGICPAEGHWLWKNGAGFSPDECAEICNPPG
                     KPNFCDLEQNCKYGTLSELGINQAVASNRIALKTLRDDVNKEECSYLCKELYVCRDSK
                     LVSVEKGEYTSLNQCVAWERLKCFGPNDPEYAACPLGTVIETEPVTGNKYWYFSEETG
                     VCKESPFFEDRDECEESLETACYSTKDECEGGLHLYYTCNPGYGCFNGKYPSTEVCEK
                     INGGKPCSESCPANCTISIGGSTCSSLPPDQGTGLDLTLSANQCITEGNSTNITWSTS
                     SQSASCGNGEILEHRTVSCKSLCGDWTVVGSIGSDIVSPDYRSEYGLQCTLDYECCTY
                     KEEKVGEPRCDSEGKNCVQETEIVRHCYDHSTVSKSKKTEVRVVSKPTIDDFKPTPPE
                     ILYTSEKIKERKHSTLSWSSSARPTGSVTQISCELDDTTYFGSNNKTEVYPQKTTNYT
                     LVCTNKDAVQIPDPECYLESDPETRTVRVFGAGIEETKPQPQSYLEGFKKLVGEIREA
                     IE"
     gene            3126..3776
                     /locus_tag="PKG74_01465"
     CDS             3126..3776
                     /locus_tag="PKG74_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOB89983.1"
                     /translation="MRSFGEVLDKIKEEAKSRLSGTDSCHEWEHTERVYNLAMRIGKK
                     EKADLDVLRLAAFLHDIARKEEDESKGKIDHAERGAILAREILEKHDLSEEKIENIVH
                     CIETHRYRNDRVPQTKEAKILYDADKLDAIGAIGLGRVFSFAGHIGAKVHNKDVDLST
                     VEPYSIEDTAWREYKIKLQYIKDKMFTDEGKKIAKERHRFMKMFFDRLNKEVDGKM"
     gene            complement(3833..>4199)
                     /locus_tag="PKG74_01470"
     CDS             complement(3833..>4199)
                     /locus_tag="PKG74_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89984.1"
                     /translation="SAELGFYKYDKPHPKEIDEAFDRTEQWNLVLNELLSSEPVWLQE
                     QVLSLGNLVGCTIVMNIREAEFITHQRTKPGVNHEVRQIALGIDRELTAAYPLWPMIS
                     RTNRTPGYAFARGTPIIEI"
BASE COUNT         1219 a          893 c         1037 g         1050 t
ORIGIN      
        1 actagcttga tctgtaatcc ttgatagttc attaaatgtc gaataggcag cctgcaaatc
       61 atctagatga aactccgaaa ttgcctttag atacattaat gaaggttgta aatagatttc
      121 tgaagtatta attaatgtag tagtaagatt taagaggtag ttccagtctc cttcatttag
      181 atttaagttc atcctttctt tgtaaaacaa gggattttag gtttattttg acgaattggc
      241 ggatttgtga tattagtgaa ttataagtcg cacattgact tcgggtgact ctagtctgaa
      301 aggaggcgga aaggagtgaa aaaagtgaga ctctgggtgg cggccgttat tttaatcgct
      361 atcggccttg gaatatttgt ctatctacaa ccaaagccca tagaacccgt ggatcctgtg
      421 gaaacagccg aagtgcctga ggtatccgac gaaccgatcg aagagatcgc agtcgcggat
      481 ctctacgaag tgccggagcc gaaagaggat ggcactttgg tagggagttt cggccctcta
      541 tttccgccgg gaacgatata cgaaattgtg tcgtccgaga ccgtatctaa acagggaagt
      601 tgggcacaga tcattcgtat taacctgtcc gcggatgaga cgaggttgtt ggcagtaagc
      661 gagggaacgg ctaaccttac cgatgataaa cggaaagatt tcgccgctct atctgcaata
      721 gcgggaacag ccaagctaga aattcaagta tgtttctccg taggatccga aatcctcttc
      781 gaggacgatt cgttcttaag gagaggagac acaatggcca tcatccgagg gaaaagtact
      841 tttgaccccg aagtaacggt ggtcatagct gtttctcctt ggaatgaagg ggataccgag
      901 cttgttgatc agtttatcca attgatcaag ctcggcagcc aaaatcccga aggttcgtaa
      961 agccttgctc tatatagagt gaggcttttt tctcgacgtc tttgctataa taggtttatg
     1021 aaaacaaaac ggattgtctc gatattttta ggaatcgtgg ttttattcgg ttgcatgata
     1081 atccgagcca cagctcgtga aatggaagag gatggttatg attggcagtt tttgtctcct
     1141 aaagaaaaac tgattcaggt tgttaatcaa gaagtctatt gcccgcgtcc atcgggatgt
     1201 gtaatgggcg aatgttttgt caccgaaagc tatgactaca ccgccggaaa ttgtccgggt
     1261 caagcttgta aagacttagt aaatgaaatt aacgccgcaa atgccacgaa taccaagaat
     1321 cttactactt taaaaggaga aggtcttctt aaatcttgga caaaatacga tttaattctt
     1381 tatacgattt ctaactggtt tttctcacca gattatggtt cttgggagtg gcacgagctt
     1441 aaagaagctt attctgatgc cgacaaatgt accaacgaca gttcttcgaa taagtgttta
     1501 gccaataatg ttggtctttg ggccgggtct tgtgaaggcg gggccggtgg attttttaaa
     1561 tgttgctgtc acaaccaaac taataaacca atttcttcag ttccggatcc ggctgctgat
     1621 aatcatccac ctctcgaggg tatttgcccg gcggaaggcc actggttatg gaagaatgga
     1681 gccggatttt ctccggacga atgtgccgaa atctgcaatc cgcctggtaa acccaatttt
     1741 tgcgatcttg aacagaattg caaatacggc accctttccg aattaggtat caaccaagcc
     1801 gtagcaagta atcgtattgc tttaaaaacc ctccgagatg acgttaataa agaagagtgt
     1861 tcttatcttt gcaaagaact ttatgtttgc cgcgattcca aattagtctc ggttgaaaaa
     1921 ggcgaataca caagccttaa ccaatgcgtg gcctgggaaa gactcaaatg ttttggtcca
     1981 aacgatccgg aatacgccgc ctgtccttta ggaacagtaa tagaaaccga accggtaact
     2041 ggaaacaaat attggtattt ttccgaagaa accggcgtgt gcaaggagag tccgtttttt
     2101 gaagacagag acgaatgcga agaatctttg gaaacggctt gttattccac taaagacgaa
     2161 tgcgagggcg gccttcatct gtattacact tgtaatccag gatacggctg ctttaacgga
     2221 aaatatcctt cgacagaggt ttgcgaaaaa atcaatggtg gcaaaccttg ttcagagtct
     2281 tgtcccgcga attgcacgat ctccatcggc ggttccacct gttcttctct cccgccggac
     2341 caaggaaccg gcctcgatct cactctttcc gctaaccaat gtatcaccga aggaaactcg
     2401 accaatatca cttggagtac ttcttctcaa agcgcaagct gcggtaatgg cgaaattctg
     2461 gaacacagaa ccgtctcctg taaatctctt tgcggagact ggacagtcgt cgggtctatc
     2521 ggctcggata tcgtaagtcc ggattaccgt tccgaatacg gtttacaatg tactcttgat
     2581 tacgaatgct gcacctataa ggaagaaaaa gtcggggaac ctcgttgcga ttccgaagga
     2641 aagaattgcg tacaagaaac ggaaatcgtc cgccattgtt acgatcactc tactgtctcc
     2701 aaatccaaaa aaaccgaggt gagagtggtt tccaaaccga ccatcgatga ttttaaaccc
     2761 actcccccgg agattcttta cacgagcgaa aagataaaag aacgtaaaca ttccaccctt
     2821 agctggtcgt cgtccgcaag accaaccggt tccgtcaccc aaatctcctg cgaattggac
     2881 gacaccactt atttcggatc gaacaataaa accgaggttt atccacaaaa aaccacgaac
     2941 tataccttag tttgtacgaa caaagacgcg gttcaaattc ccgaccccga atgctatctc
     3001 gaatccgatc cggagacgag aaccgtcagg gttttcgggg cgggaataga ggagaccaag
     3061 ccgcaacccc aaagctatct tgaaggtttt aaaaagctgg taggcgagat ccgggaggcg
     3121 atagaatgag aagctttgga gaggtactgg ataaaataaa ggaagaagcc aaaagcagat
     3181 tatccggcac agacagctgt cacgagtggg agcatacgga aagagtctat aatttggcga
     3241 tgcgcatcgg taaaaaagaa aaagccgatt tggatgtttt gcgtttggcg gcctttttgc
     3301 acgacatcgc cagaaaagaa gaagacgagt caaaaggaaa aatcgatcac gccgaaaggg
     3361 gtgcgattct cgcgagagaa atcttggaaa aacacgattt gtcggaagaa aagatagaga
     3421 atatcgtgca ttgcatcgaa actcaccgtt atagaaacga tcgggttccg cagacgaaag
     3481 aagccaaaat cttatacgac gccgacaaac tggacgccat aggagccatt ggtctcggcc
     3541 gggtgttttc tttcgccgga catatcggcg ccaaggtgca taacaaggat gtcgatctaa
     3601 gcacggtcga accatactcg atcgaagaca cggcttggag ggaatacaaa ataaaactgc
     3661 agtacataaa agacaaaatg tttaccgacg aaggtaaaaa gatagcgaaa gaacgtcatc
     3721 gttttatgaa gatgtttttc gatcgattga acaaggaggt ggatggcaag atgtgaagta
     3781 aaaataaagg aaacggcgtc cgtggggacg ccgttttgga aagggcaaag tttcagatct
     3841 cgatgatcgg agttccccgg gcaaaggcat agcccggagt ccggttggtc cgggagatca
     3901 taggccataa gggataggcc gcggtcagct ctctgtcgat gccgagagcg atctgacgca
     3961 cctcgtggtt tacacccggc ttggttctct ggtgggtgat gaattccgct tcgcggatat
     4021 tcatgactat agtgcagccg acgagattcc cgagggagag aacctgttct tggagccaaa
     4081 ccggctccga agaaagaagt tcgttcaaaa cgaggttcca ctgctccgtg cggtcaaagg
     4141 cttcgtcgat ctccttcgga tggggtttgt cgtatttgta gaatccgagc tcggccgac
//
LOCUS       DAOPQT010000040         4144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_23336_length_4144_cov_2.993152, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000040 DAOPQT010000000
VERSION     DAOPQT010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23336_length_4144_cov_2.993152"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PKG74_01475"
     CDS             <1..598
                     /locus_tag="PKG74_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOB89985.1"
                     /translation="AESYYNFEALPSLVRPGKFLAKPKAEYKSYAFSENGVSPRLVPG
                     QGPIVRATSYEHDEFGLTVEDPETIEAMNEKRWKKAKFLEKEIAKLNPCRVYGKGKNL
                     IIGWGSTKGAVLDALRQLPDYSFLQISYVAPFPVKETEKAIRKAKRTILLENNSTGLL
                     GRILKEETGIEIKDKLLKNNGRPFTADEIARLLKNRHE"
     gene            591..1334
                     /locus_tag="PKG74_01480"
     CDS             591..1334
                     /locus_tag="PKG74_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOB89986.1"
                     /translation="MNKKKDFGTKMPITWCPGCPNNIVLYVLKSVFGELIDAKKIRKQ
                     DIVIVTDIGCAGKIYDYLDVNAFYGLHGRAIPIATGIKIGNPKLTVVAVAGDGGTYSE
                     GLNHLVSSGRLNPDITVIVINNGVLALTKGQPTSTHPSIKGEPIFDPVPLALASEASF
                     VSRETTFDLRDLQSTVKAAITHKGFSLVDIFEPCLSYNNDGERIRDTERVCDDSLQSG
                     FDKRLRKIRTGVFRKISRPTLEQKLKKKI"
     gene            1331..1561
                     /locus_tag="PKG74_01485"
     CDS             1331..1561
                     /locus_tag="PKG74_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOB89987.1"
                     /translation="MKKIILDYGKCAHCGLCANVCPEVFGWDAKSSEPFLKNKSKKEQ
                     GRASGETDNPCAEEAARLCPVAAIEIANLKKK"
     gene            1675..2067
                     /locus_tag="PKG74_01490"
     CDS             1675..2067
                     /locus_tag="PKG74_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89988.1"
                     /translation="MQKATKCICGIIVVLVFVCLGLWAWPMTSWADYYAVAVETGDVY
                     FGKLGRFPNLQLRDVWFLQQTGDEENPYSLVKFTDLRWSPEDKLELNYDRVVWIVKLN
                     ELGQVGTFLKNPALRQSNPVSQPPTETP"
     gene            2136..2807
                     /locus_tag="PKG74_01495"
     CDS             2136..2807
                     /locus_tag="PKG74_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89989.1"
                     /translation="MALIALLPMAALIFGLSLIFSLLLFPLFRLLFHRLFVENHELEI
                     IEIFPDKDEKAVAKIIDKVDFKSAIEISVRHLGKDTEYYMIVPKAFLERAKKFIKEVL
                     PNTRFKKTEDYPLFNYSGVTLAKAVVAESEAQVRDIDFGKIDFSKVNEVGEGAVVQIV
                     HTPRVPGVNFEIRILSSAPSEYQAREIMDTIADSGFKGIKVREPKDKVKFIHTINFRE
                     HSFGL"
     gene            2944..3894
                     /gene="murB"
                     /locus_tag="PKG74_01500"
     CDS             2944..3894
                     /gene="murB"
                     /locus_tag="PKG74_01500"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOB89990.1"
                     /translation="MIIQENILLKNHTSYKIGGPARYFAEAKSIADIKEALEFWKKEG
                     MALFVLAGGNNVIFSDSGFAGLVLKVSLDSIKVSGEEITAEAGAKLSDVVELASKNSL
                     KGIEWAGGLPGTIGGAVFGNAGAFGGEMKDIVVAVKSLDIRNPEKIVSRKNSECGFGY
                     RTSVFKSIRNEIILSIELRLQPGKSEEIEKAIKEHIGYRIERQPLEYPSAGSTFKNID
                     TNLVSEEQKKEWSAVMKTDPFPLVPVAHLLSEAGLKGKRVGGAMISEKHPNFFINVGG
                     ATASDVLDLISLAKKTIKEKFGIELEEEVQIVDENGGIRF"
BASE COUNT         1238 a          934 c         1046 g          926 t
ORIGIN      
        1 ggcggaaagt tattataatt tcgaagcttt gccttcgctg gtccggccgg gtaaattcct
       61 ggcaaaacca aaagcggaat acaaaagcta tgctttttcc gaaaacggag tcagtccgcg
      121 cctcgtaccg gggcaaggtc cgatcgttcg ggccacgagc tacgaacacg acgaatttgg
      181 gcttaccgtc gaagatccgg aaacgatcga ggcgatgaac gaaaaacgct ggaaaaaagc
      241 caaattcttg gaaaaggaaa tcgcgaagtt gaatccttgc cgcgtttacg gcaaggggaa
      301 aaatctgatc atcggttggg gatcgactaa aggcgcggtt cttgatgcct tgagacaatt
      361 gcccgactat tcttttctcc agatttctta tgtcgcgccg tttccggtaa aagaaacgga
      421 aaaagccatt cgaaaagcga aaagaacgat tcttttggaa aacaattcga ccgggcttct
      481 cggccggatc ctcaaagaag agacgggtat cgaaatcaaa gacaaattgc tgaaaaacaa
      541 cggccggccg ttcaccgccg acgaaatagc aaggctattg aaaaaccgac atgaataaga
      601 aaaaagattt tggtacgaaa atgccgatca cctggtgtcc gggatgcccg aacaacatcg
      661 tcctttacgt attgaaatcc gtcttcggag aactgatcga cgcgaaaaaa atcaggaagc
      721 aggatatagt catcgtcacc gatatcggct gcgccggaaa aatatacgac tatttggacg
      781 tgaacgcttt ttacggattg cacggacgag ccataccgat cgccaccggc atcaagatcg
      841 gaaatccgaa attgacggtc gtcgccgtcg ccggagacgg cggaacctac agcgagggat
      901 tgaatcattt ggtctcgagc ggccggttga atccggacat caccgtcatc gtcatcaata
      961 acggcgttct cgccctgacc aagggccagc cgacgtcgac ccatccgtcg atcaaaggag
     1021 aaccgatctt cgatcctgtt ccgttggcct tggcgagcga ggcgtctttc gtcagccggg
     1081 aaacgacctt tgatcttcga gatctccaat cgaccgtcaa agcggccata acacacaaag
     1141 gattttctct ggtcgacata ttcgagccgt gtttatctta taataacgac ggcgaacgga
     1201 tacgcgatac ggaaagagtc tgcgacgact ctcttcaatc gggttttgat aagagactaa
     1261 gaaagattcg aacgggcgtg ttccggaaaa tatcccgacc tactctggaa caaaaactga
     1321 agaaaaaaat atgaaaaaaa taattttgga ttacggaaaa tgcgcgcatt gcggactttg
     1381 cgccaatgtc tgcccggagg ttttcgggtg ggatgccaaa tcatccgagc cgtttctgaa
     1441 aaacaaaagc aaaaaagaac aagggagggc gtcgggggaa accgacaatc cctgcgccga
     1501 ggaagcagcc agactttgtc cggtggccgc catcgaaatc gcaaatttga agaaaaaata
     1561 atttgaccct ttttacagga agatttaaaa ttacaaagaa gtcgatatta aaaaccaaaa
     1621 tagtcggatc gataaatttt taaaaaaata atgagaggcc gttccaataa aaaaatgcaa
     1681 aaagcaacca agtgtatctg cggaatcatc gtcgttcttg tcttcgtctg cctggggttg
     1741 tgggcgtggc cgatgacttc gtgggccgat tattacgccg tggccgtgga gaccggagac
     1801 gtttacttcg gcaagcttgg ccgtttcccg aatcttcaat tgagagacgt ctggttcctg
     1861 cagcaaaccg gagacgaaga aaatccttac agtctggtga aattcacgga tttgcgctgg
     1921 tcgccggaag ataaactcga actgaattac gatcgcgtcg tttggatagt caaactgaac
     1981 gaactcggcc aggtcggaac tttcctgaaa aacccggctt tgcggcagtc gaatcccgtg
     2041 tcgcaaccgc ccacggaaac accgtaaccg cttcaatgaa aatcccccgc aacgggggat
     2101 ttttgaagcg gccaagctgt tgtataatga agacaatggc acttatagct ttattgccga
     2161 tggccgcgct tattttcgga ttgtccctga ttttctccct gcttcttttt ccgttgttcc
     2221 gtttgctttt ccatcggctt ttcgtggaaa atcacgagct agagatcata gagattttcc
     2281 cggataaaga cgagaaagcc gtcgccaaga ttatcgataa ggtggatttc aaatcggcca
     2341 tcgaaatctc ggtccgccat ctgggcaaag acaccgaata ctacatgatc gtcccgaaag
     2401 ctttcctgga gcgagccaag aaattcatca aagaagtttt gccgaacacg agattcaaaa
     2461 agacggaaga ttatccgctt ttcaattact cgggcgttac cttggccaag gcggtggtgg
     2521 cggaatccga agcccaggtt cgggacatcg atttcgggaa aatcgatttt tccaaggtga
     2581 acgaagtcgg cgaaggagcg gtcgtccaga tcgtccacac cccccgggtg ccgggagtta
     2641 atttcgagat caggattcta tcttcggctc cgagcgagta tcaagcgagg gagatcatgg
     2701 acacgatcgc cgattccggg ttcaagggaa taaaagtcag ggagccgaaa gacaaagtga
     2761 agtttatcca cacgataaat ttccgggagc acagctttgg gttataattg attcgagcgg
     2821 caggacgagc actcgctaaa cacaaatagt ctttgtacga ggtcaccact gccgctcttt
     2881 tcgtatccaa aaactccgat ttcttttttg acttttgcgc cgaatagccg gagactatag
     2941 acgatgatca tccaggaaaa tattcttctc aaaaaccata cgagttacaa aatcggcggt
     3001 ccggccaggt atttcgcgga agcgaaaagc atcgccgata tcaaggaagc gctcgagttt
     3061 tggaaaaaag agggaatggc gctttttgtt ttggccgggg gaaacaacgt tattttttcc
     3121 gacagcggct tcgccggtct ggttttgaaa gtttcccttg actccatcaa agtatccgga
     3181 gaagaaatca cggccgaagc cggagcgaaa ctcagcgatg tcgtcgaatt ggcgtcgaaa
     3241 aacagtttga agggaatcga atgggccgga ggcttgccgg gaacgatcgg cggcgcggtt
     3301 ttcggcaacg ccggcgcttt cggcggcgaa atgaaagata tagtggtcgc agtaaaaagc
     3361 ctcgatatca gaaacccgga gaaaatcgtc tcgaggaaaa atagtgagtg cggtttcggt
     3421 taccgcacga gtgtttttaa aagtatcagg aacgaaatca ttctctcgat cgagcttcgg
     3481 ctgcaaccag gaaaatccga agaaatagaa aaagccatca aagagcacat cggctatcga
     3541 atagaaaggc agccgctcga atatccttcg gccggaagca ccttcaagaa cattgacacc
     3601 aatctcgtaa gcgaagaaca aaaaaaagaa tggagcgcgg tcatgaaaac cgatccgttt
     3661 ccgctcgtgc ccgtcgctca tcttctttcc gaagccggac tcaagggaaa gcgcgtcggc
     3721 ggagcgatga tttcggaaaa acacccgaat tttttcatca atgtcggcgg agcgaccgcg
     3781 agcgacgtgc tcgatctcat ttcgttggcc aaaaaaacga tcaaagagaa attcggaata
     3841 gaattggaag aagaagtgca gatagtcgac gagaacggag gaataagatt ttaagatcgg
     3901 gaattttgcc gggaaaaaac aaaaattttt ccggaatcaa aaaattttga gttatccaca
     3961 tgttttttaa ataccatttg ttctaaaatt atatcgaagg ttgatgaaat tggttggtcg
     4021 acaattcttt atcaacttgt cgtgaataaa aaaatctgaa atggctaaaa aagctaaaaa
     4081 ggccaagaaa gcgaagaaga tgactaagaa ggccaaaaag acaaccaaga agactaagaa
     4141 gaga
//
LOCUS       DAOPQT010000041         4139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_23385_length_4139_cov_3.597943, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000041 DAOPQT010000000
VERSION     DAOPQT010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23385_length_4139_cov_3.597943"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1058
                     /locus_tag="PKG74_01505"
     CDS             186..1058
                     /locus_tag="PKG74_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOB89991.1"
                     /translation="MEKTRKKVGLALGGGGARGFAHIGIIKALNDLGIPIDFIAGTSM
                     GAIIGGLYALTESVESIESKIKGLEGKNLKLVNAIAGKSAKPIAKNQSMLKTIDEFFG
                     DAKFEDCPVPFKAVATDLKTGDEMSLDQGLMAPAVKASAAIPMIFQPVEIDGKLLVDG
                     GLARPIPADVVKQMGADVVIAVDVSAKWVDISADLTKGIGWQGFYSVLSNMLAAMNQQ
                     ISKEILKNNADITLRPAVTGYDWLRFGNPEDIIASGYREAYSQKALIFEKTGYEPSAK
                     TGLQIFLDFLFGLD"
     gene            1061..2245
                     /locus_tag="PKG74_01510"
     CDS             1061..2245
                     /locus_tag="PKG74_01510"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOB89992.1"
                     /translation="MNKFYEKLPDTPGVYLMKNGRGEIIYVGKAANLKRRVSSYFTRP
                     HDSRLEKLVSEIKKIDHRRTDTALEALILEVELIKRYRPFFNIKDKDDKSFLYFEITK
                     DVYPRVMLVRGRETKKGERFGPFTSASSAREAGKILRRIFPWNIHPPEKIGKFKRPCF
                     DFEIGLCPGTCVGLADKVGYRKNISNLKMFLKGKKKTLIKNLEKEMSKASEALDFEKA
                     EKIKRQIFSLNHIQDIALIKDNEFPTGEKIEFRVEGYDISNISGDSAVGSMVVWENGD
                     LNPNEYRKFRIRTIKQADDTGMMKEMITRRLKNNWPLPDLILVDGGLGQMSAARAALS
                     EAGLKIPIVGIAKGPTRKADRLIGALPRSVSKNDLLSLRDEAHRFAISYHKKVRGEKF
                     LS"
     gene            2296..2550
                     /locus_tag="PKG74_01515"
     CDS             2296..2550
                     /locus_tag="PKG74_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOB89993.1"
                     /translation="MEKEFLSTTEAAKLLNISRVAVFKKIKSGDIKAERIGRNFVISK
                     KDLPEILGTTLGEAKRNLIEKAVRKTIKDYGETLRLLGEE"
     gene            2547..2975
                     /locus_tag="PKG74_01520"
     CDS             2547..2975
                     /locus_tag="PKG74_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HOB89994.1"
                     /translation="MKSKSITLIEVENVAYTLARELMTYNEPIPEFNSRFPNILESCL
                     AVPFQTFDKKLVYKGLIGKASILFYLLIKNHPFQNGNKRIAITTLMVFLYINNKWIEV
                     DNQEFYNFAKWVAASNPKVKEATVAAIEKFLETYIVKLVN"
     gene            3014..4018
                     /gene="ychF"
                     /locus_tag="PKG74_01525"
     CDS             3014..4018
                     /gene="ychF"
                     /locus_tag="PKG74_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOB89995.1"
                     /translation="MKLSIGIVGLPNVGKSTLFKALTKQEVNVANYPFATIDPNVGVV
                     AVPDRRLDTLAEMSDSKKKIPAVVEFYDIAGLVKNAYQGEGLGNQFLANIRETNAIVQ
                     VVRCFESSEIIHVENGVDPVRDIDIINTELIFKDLETTTKRLGSLEKEARTGDKQKIR
                     ELEVLRKIKSVLETGNLIVNLEDVKTEPIVKELGFLTAKPQIYLLNGDSEDIKPELRE
                     KIKSLNADFVVENLSASVDLSELIRKSYEVLGLISFFTTGEDETRAWTVEKGTKAPQA
                     AGTIHTDFEKNFIRLEVVEYLKLVEAGAWVNARQKGWIRLEGKDYLVEDGDVVVVRHG
                     "
BASE COUNT         1213 a          910 c         1069 g          947 t
ORIGIN      
        1 cttgacgtca tcaagtccgc ggacttcgta atcgacctcg gccccgaagg cggagaaaac
       61 ggcggccgag tggtggcttt cggcactccg gaagaagtgg cgagaaaaaa atcaagccat
      121 accggagaat atctgcgtaa agtgttggat tagtctgaga gttttaaggt agaatgcaga
      181 cgatcatgga aaaaacacgg aagaaagtcg gattggcttt gggcggcggc ggagccaggg
      241 gatttgccca tatcggtatc attaaggctt tgaacgattt gggaataccg atcgatttca
      301 tcgccggcac gagcatggga gcgatcatcg gcggactcta cgctttgacc gaatccgtcg
      361 agtccatcga aagtaaaata aaaggattgg aagggaagaa tctcaaattg gtgaacgcga
      421 ttgccggaaa atccgcgaag ccgatcgcga aaaaccaatc gatgctgaaa acgatcgacg
      481 agtttttcgg agacgcgaaa ttcgaagatt gccccgtgcc tttcaaggcg gttgccaccg
      541 accttaaaac cggcgacgag atgagtctcg atcaaggatt aatggctccg gcggtgaaag
      601 cgagcgccgc cattccgatg attttccagc ccgtggagat cgacgggaaa ctgcttgtcg
      661 acggcggatt ggcgagaccc attccggcgg acgtcgtgaa acaaatggga gccgacgtcg
      721 tcatcgccgt cgacgtttcc gccaaatggg tggatatttc cgcggatctc accaaaggta
      781 ttggctggca gggtttttac agcgttttga gcaacatgct cgcggccatg aatcagcaga
      841 tttccaaaga gattttgaag aataacgccg acatcacgct tcgcccggcg gttacgggat
      901 atgattggct gaggttcgga aatccggaag atatcatcgc ttccggctat cgcgaagcct
      961 attcccagaa ggcgttgatt ttcgagaaaa ccggatatga gccgtcggcc aaaaccggcc
     1021 tccagatttt tttggatttt cttttcggcc tcgactgaaa atgaacaaat tttacgaaaa
     1081 acttcccgac actcccggag tgtacttgat gaagaacggc cgcggagaga tcatctatgt
     1141 cggaaaagcg gccaacctga agcgaagggt ttcgagttat ttcacgcggc cgcacgacag
     1201 ccgcttggaa aaactggttt cggaaatcaa aaaaatagat catcgccgaa ccgacaccgc
     1261 cttggaggcc cttattctcg aagtcgaact gatcaagaga taccggccgt ttttcaacat
     1321 caaagacaag gatgacaaga gttttcttta tttcgaaatc accaaagacg tctatccgcg
     1381 ggtgatgctt gtccgcggtc gggaaactaa aaaaggcgaa aggttcggac cgttcacttc
     1441 ggcctcttcc gccagggaag cgggaaaaat actccgaagg atttttccgt ggaacatcca
     1501 tccgccggaa aaaatcggga aattcaaacg gccgtgcttc gatttcgaaa tcggactttg
     1561 cccgggaaca tgcgtcggac tcgcggataa ggtcggctac cgcaagaata tctccaatct
     1621 gaaaatgttt ctgaaaggaa agaaaaagac tttgatcaaa aatctggaaa aagagatgtc
     1681 aaaggcatcg gaagcgctcg atttcgagaa agccgagaag atcaagcgtc agatcttcag
     1741 tttgaatcac atccaggaca tcgccttgat taaagacaac gaatttccga ccggagaaaa
     1801 aatcgaattc cgcgtcgaag gatacgatat ttcgaatatt tccggggatt cggccgtggg
     1861 cagcatggtg gtctgggaaa acggggattt gaatccgaac gaataccgta aattcaggat
     1921 aagaacgata aaacaagcgg acgacaccgg gatgatgaaa gaaatgatca ctcggaggct
     1981 taaaaacaat tggcctttgc cggatctgat tttggtcgac ggcggactcg gacaaatgtc
     2041 tgccgcgaga gccgctttgt ccgaagccgg actgaaaata ccgatcgtgg gcattgccaa
     2101 aggcccgacg cgaaaagccg ataggctgat cggcgctttg ccgagatccg tgtctaaaaa
     2161 cgatttatta agccttcgcg acgaagccca tagatttgcc atcagctatc ataagaaggt
     2221 gaggggagaa aaattcttaa gttaacatat gttaaccatt gactactcgg ttaacatagg
     2281 ctataatcga tttaaatgga gaaagaattt ttatccacaa ccgaagcggc caaactgttg
     2341 aatatcagcc gtgttgcggt attcaagaaa atcaaatccg gagacatcaa agccgagaga
     2401 ataggccgca attttgttat ttccaaaaaa gacttgccgg agatattagg aacgactctt
     2461 ggcgaagcaa aaagaaactt aatcgagaaa gccgtccgaa aaacgattaa agattacggt
     2521 gagacgcttc gattattggg agaggaatga aatcaaaatc gattactctt atagaggtgg
     2581 agaacgtggc ttacacgctg gctagggagt tgatgacgta taacgaaccg attccagagt
     2641 ttaattcccg ttttcccaat atcctcgaga gttgcttagc cgttccgttt cagacattcg
     2701 ataagaagtt ggtttataag ggtcttatcg gtaaggcttc gattttgttc tatcttctta
     2761 ttaaaaatca tcctttccaa aacggtaata agcgtatcgc catcactacg cttatggttt
     2821 ttttatatat aaataataaa tggatagaag tcgataatca ggaattttat aatttcgcga
     2881 agtgggttgc cgcgagcaat ccgaaagtaa aagaggcgac cgtagcggct atagaaaaat
     2941 tcctggaaac gtatatagtc aagcttgtaa attaagtaat gaatctcgtt agagacgatc
     3001 gattaatcat aaaatgaaac tttccatcgg tatcgtcggt cttccgaatg ttggcaaatc
     3061 cacgcttttt aaggctttga ccaagcagga agtgaatgtc gccaattatc ctttcgccac
     3121 catcgatccg aacgtgggcg tggtggccgt gcccgaccgc aggctcgaca cgctcgccga
     3181 aatgagcgat tccaagaaaa agattccggc ggtcgtcgag ttttacgaca tcgccggcct
     3241 ggttaaaaac gcttaccagg gcgaaggttt gggaaaccag tttttggcga atatcaggga
     3301 aacgaacgcc attgtccagg tggttcgctg tttcgagtct tcggaaatca tccacgtgga
     3361 aaacggagtc gatccggtcc gcgacatcga catcatcaac accgaattga ttttcaagga
     3421 tttggaaaca acaaccaagc gtttgggaag cctcgagaaa gaagcgcgca ccggagataa
     3481 gcagaagatc cgcgagctcg aagttttgag aaaaataaaa tcggtcttgg agaccggtaa
     3541 tttgatcgtt aatttggaag acgttaaaac cgagccgatc gtcaaagaac tcgggttttt
     3601 gaccgccaag ccgcagatct atcttttgaa cggcgattcc gaggacatca agccggaatt
     3661 gagagaaaaa ataaaatccc tgaatgccga tttcgtcgtg gaaaatctct ccgcctcggt
     3721 tgacctttcg gagctaatca ggaaatccta cgaggtcttg gggctgatca gttttttcac
     3781 gaccggcgag gacgaaaccc gcgcctggac cgtggaaaaa ggaaccaaag cgccgcaagc
     3841 ggccggaacg atccacaccg atttcgagaa gaatttcatc cggttggaag tggtcgaata
     3901 tctgaagctg gtcgaggctg gagcctgggt gaacgccagg caaaaaggct ggatccggct
     3961 tgaaggaaaa gactatttag tcgaagacgg ggatgttgtt gtcgtcaggc acggataaac
     4021 cgattcgaaa cagcacaatt tttgagctat aattcaaagg ttccgacatc atgtttcttc
     4081 agaagctcga actgcaagga ttcaaatctt tcgccaacaa aaccgttttg gactttccc
//
LOCUS       DAOPQT010000042         4114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_23559_length_4114_cov_3.591032, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000042 DAOPQT010000000
VERSION     DAOPQT010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23559_length_4114_cov_3.591032"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1063
                     /gene="miaA"
                     /locus_tag="PKG74_01530"
     CDS             149..1063
                     /gene="miaA"
                     /locus_tag="PKG74_01530"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOB89996.1"
                     /translation="MIKTDKKIIVVLGPTASGKSDLAVELAIKHNGEIVSADSRQVYK
                     GLDIGSGKISEKEMRGIPHHLLDVASPRRIFTASDYGQLSKKAIDDILRRGKLPIVCG
                     GTGFYIDALLYDDAIAPIPPNPKIRKTIEKLSTEKLAAKLSDIDPDRFASIDRKNRVR
                     LIRALEIVMASGKPVPKPKRRARYDTLKIGISYPMEKLDERIEVRLDKRLQSGMVDEV
                     KRLYKNGLSWKRLFDLGLEYRYISLYLRGQLSCEEMRQELLKAIRQYARRQMTWFKKD
                     QTIKWIKDKSEADRLLEEFLKTDISAKL"
     gene            complement(1060..2538)
                     /gene="gatB"
                     /locus_tag="PKG74_01535"
     CDS             complement(1060..2538)
                     /gene="gatB"
                     /locus_tag="PKG74_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOB89997.1"
                     /translation="MKYSPTIGLEIHVELNTKTKMFCGCRNNSLETKPNTNVCPICLG
                     HPGTLPVPNIEAVKSVLKLGLALGSDISEKSKFDRKSYFYPDLPKGYQISQYDEPLVK
                     NGELLGVRVRRIHLEEDVGRLLHEIPAENGNRPKETDKATYVDFNRAGVPLMELVTEP
                     DIESGEQAVAFGKMLQLILRYLRISAADMEKGQMRVEANVSVRPEKQREYGTKVEVKN
                     LNSFRSVGDAVNFEIKRQTEVLESGGKVAQETRGWDKNKKETFSQRSKEEAKDYRYFP
                     EPDIPAIDFRETSLVDFEELRRSIPELPLQKRSRLMSEYGLRPIQADLMIEDRESTDF
                     FEATLSEALGDKNIQAPERQRFIDLVFNYLTSDLKGFMNAKGIAFSDLKIDPENFADF
                     IDLIFDGDISSRIAKDLLPEMQRSGLDPKQIIKEKGMEQVLDEEIIKSAVQKVIKDNP
                     SAVSDYKAGKKTAFQFLIGKAMAELQGKGHPGKISELLEKEL"
     gene            complement(2603..3325)
                     /locus_tag="PKG74_01540"
     CDS             complement(2603..3325)
                     /locus_tag="PKG74_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB89998.1"
                     /translation="MMEKKIPGGLMIVFGGTDGTGKNTESQFAEKWFKGQGYAVAKFA
                     FPRYGSPAGWFVAAYLGKLPDHTPPRYGPPNDVPPKIASEFYALDRYDASFEIRQALQ
                     DGKVVICDRYVESNAGHQGGKISDSEERKKYVDELFDREYGFYGIPRPDINIILWLPP
                     ELVEQRIVHRGTPQGHEVDPKHMINAGRSYRWLTETYPDFILVECSDKNGENLSVEAV
                     DAKVEAILRRVLDSKLENVKNS"
     gene            complement(3333..3896)
                     /gene="dcd"
                     /locus_tag="PKG74_01545"
     CDS             complement(3333..3896)
                     /gene="dcd"
                     /locus_tag="PKG74_01545"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOB89999.1"
                     /translation="MVLSDRDIKKVIKQKRLVFLPKLSPDQIGPASIDLKLGQQFKVF
                     HISETSLLDTKKGIPLKVMRNYDNRESFILHPGKFILASTAEYMKVPSDMVLRVEGKS
                     TLARMGILVHTAGFVDPGFEGAITLEISNQSDLPVKLYPGMYICQVAVEYLSSPAERP
                     YNLRKKSLYNKQKGPTAANPKNLFGKL"
     gene            complement(3927..>4114)
                     /locus_tag="PKG74_01550"
     CDS             complement(3927..>4114)
                     /locus_tag="PKG74_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90000.1"
                     /translation="EIPAEVTDVIVFACKVANIAEDFHGTEKLEQVMKKKLEYCETRT
                     YNPEINKMDKPENREFN"
BASE COUNT          981 a         1020 c          980 g         1133 t
ORIGIN      
        1 gaaggggatc tcggccaaaa accgctcgac tcgtttatcg ccgacctgaa acaagagatc
       61 aaagacaaga aataatcaga ccccatccaa aaccccttca aaatgaaggg gttttgttaa
      121 cccggtaaaa ctcgtataaa ttaaaatagt gattaaaacc gacaaaaaaa tcatcgtcgt
      181 cttggggcca accgcttcgg gtaaatccga tctggcggtc gaactagcca taaaacacaa
      241 tggagagatt gtttccgccg attcaagaca ggtttacaaa ggtttggaca tcggttcggg
      301 aaaaatctcc gaaaaagaaa tgaggggcat tccccatcat cttttggatg tggcttctcc
      361 cagaagaatc tttacggcat cggattatgg tcagcttagt aaaaaagcga tcgatgatat
      421 tttgcgtcgc ggcaaactcc cgattgtttg cggcggcacg ggattttata tcgacgcttt
      481 actctacgac gacgcaatcg ctccgattcc gccgaatcct aaaataagaa aaactatcga
      541 gaaactttca accgagaaac tggccgcaaa attatcggat attgatccgg accgtttcgc
      601 ttcgatcgat cgaaaaaaca gggttcggct tattcgggct ctcgagatcg tgatggcatc
      661 cggaaaaccc gtcccgaaac cgaaacgccg ggctcgctac gataccttga aaatcggcat
      721 ttcttatccg atggaaaagc tcgacgaacg gatcgaggtt cgtctagaca agcgccttca
      781 atcgggcatg gtggacgagg tgaagcggct ttataaaaac ggcctttcct ggaaacgtct
      841 tttcgatctc ggactcgaat accgatacat ctcgctctat cttcgcggac agttaagctg
      901 tgaagaaatg cggcaagagc ttttgaaggc catccggcaa tacgcgaggc gccaaatgac
      961 ctggttcaag aaagatcaga cgatcaaatg gattaaagat aaatcggaag ccgatcggct
     1021 tctcgaagaa ttcctgaaaa cggatatttc cgcaaaactt taaagctctt tttcgagcaa
     1081 ttcgctgatt ttcccggggt ggcctttacc ttgcagttcg gccatggctt tgccgatcag
     1141 gaactggaaa gcggttttct ttccagcttt gtaatcactg accgcgctgg gattgtcttt
     1201 gatgactttc tggaccgcgg atttgatgat ttcttcgtcc aaaacctgct ccattccttt
     1261 ttcttttatg atctgcttcg gatcgagtcc cgaacgctgc atttccggaa gcaaatcctt
     1321 ggcgatccgg ctcgatatgt cgccgtcgaa aatcaaatcg atgaaatccg cgaaattctc
     1381 cggatcgatt ttgagatcgc tgaaagcgat tcctttggcg ttcatgaagc ccttgaggtc
     1441 gctcgtcagg taattgaaaa caaggtcgat gaatctttgt ctttccggag cctggatatt
     1501 cttgtctccc aaggcttcgg aaagcgtggc ctcgaaaaaa tcggtcgatt cccggtcttc
     1561 gatcatcaaa tcggcttgga ttggccggag cccgtattcg ctcatcagcc ttgaccgttt
     1621 ttggagcggc aattccggaa tggatcgcct caattcctcg aaatcgacaa gtgaagtctc
     1681 cctgaaatcg atcgctggaa tgtccggttc cggaaaatag cgatagtctt tggcttcctc
     1741 tttgctccgc tggctaaagg tttctttttt attcttatcc caaccgcggg tttcctgggc
     1801 cacttttcct ccggactcca gaacttctgt ctggcgtttg atttcgaaat tgacggcgtc
     1861 tccgacggac ctgaaagaat tcaggttttt tacctcaacc ttggttccgt attctctttg
     1921 tttttccgga cgcacggaaa cattggcctc cacccgcatt tgtccttttt ccatgtcggc
     1981 cgcggaaatc cgaagatagc ggaggatcag ttgaagcatt ttcccgaaag cgacggcctg
     2041 ttccccgctt tctatatccg gttcggtcac gagttccatc aaaggaaccc cggcccggtt
     2101 gaaatcgaca taggtggctt tgtccgtttc tttcggtcgg tttccgtttt ccgccggaat
     2161 ttcgtgtagc agccggccga cgtcctcttc caaatggatc cttctcacac ggacaccaag
     2221 aagctcgccg tttttaacca aaggttcgtc gtattgggaa atctggtagc ctttcggaag
     2281 atcgggataa aaatagcttt tgcggtcgaa tttcgatttc tcggaaatat cggatccgag
     2341 cgccaaacca agttttaaga cggatttgac ggcttcgata ttcggcacag gcaaagttcc
     2401 cggatggccg agacagatcg gacagacgtt ggtattcggc ttggtctcca ggctgttatt
     2461 tctgcagccg cagaacattt tggttttggt gttgagctcg acgtggatct cgaggccgat
     2521 ggtcggtgaa tatttcattg aataatctta ttttatcagg tttcggagac taataaaaac
     2581 gccctttttc aagggctttt gatcagctgt ttttgacatt ctcgagtttg gagtcgagga
     2641 ctctccgcaa aatcgcctcg actttggcgt cgaccgcttc cacggacaga ttttcgccgt
     2701 ttttgtcgga acattcgacg agaataaaat ccggataagt ttcggtaagc caacgatagg
     2761 atcggccggc gtttatcatg tgtttcggat cgacttcgtg gccctgggga gttccccgat
     2821 ggacgatcct ctgctccacc agttccggcg gcagccaaag aataatattt atgtccggcc
     2881 tcgggattcc gtaaaatccg tattcccgat cgaaaagctc gtcgacgtat tttttgcgtt
     2941 cttccgaatc gctgatcttt cctccttgat gcccggcatt cgactcgaca tagcggtcgc
     3001 aaataaccac cttgccatct tggagcgcct gtctgatttc gaaactcgca tcgtaccgat
     3061 cgagagcgta aaattccgaa gcgattttcg gaggaacgtc gttcggcggt ccgtaacgcg
     3121 gcggcgtatg gtccggcagc ttgccgagat aggcggccac aaaccaaccg gccggagatc
     3181 cgtatcgggg aaaagcgaat ttggctaccg cgtagccttg gcccttaaac cacttttcgg
     3241 cgaattgcga ctcggtattt ttacccgtgc cgtcggtgcc gccgaaaacg atcatcaatc
     3301 ctccgggtat tttcttttcc atcatagata aatcaaagtt tcccgaaaag attcttcgga
     3361 ttagccgcgg tcggaccttt ttgcttgttg taaagggatt tcttgcggag attgtaaggt
     3421 ctttccgccg ggctcgaaag atattccacc gccacctggc atatatacat tccgggatag
     3481 agctttaccg gcagatccga ttggttgctg atttccaaag tgatcgctcc ttcgaatccg
     3541 ggatcgacaa acccggccgt atggacgagg attcccatcc gagccaaagt gctcttgccc
     3601 tcgacccgaa ggaccatatc ggacggcacc ttcatatatt cggccgtgct cgccaaaatg
     3661 aattttccgg gatgcaaaat aaacgactcg cgattgtcgt aattcctcat aaccttcaaa
     3721 ggaatgccct ttttggtgtc gagcaaactg gtctcgctga tatggaagac tttaaattgc
     3781 tgtccgagct tgagatcgat cgaggccgga ccgatctgat ccggagaaag tttcggaaga
     3841 aaaaccagcc tcttttgctt gatgactttt ttgatatccc tgtcggagag aaccattaag
     3901 ctcggtttat ttgttattcg acgacattag ttaaattccc tgttttcggg cttgtccatt
     3961 ttatttattt ccggattata ggttctggtt tcacaatatt ccagtttctt tttcatgact
     4021 tgttccaatt tttccgttcc atgaaagtcc tcggctatat tggcgacttt acaggcaaaa
     4081 acgatcacat cggtaacctc ggccgggatc tctt
//
LOCUS       DAOPQT010000043         3990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_24483_length_3990_cov_4.010673, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000043 DAOPQT010000000
VERSION     DAOPQT010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24483_length_3990_cov_4.010673"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="PKG74_01555"
     CDS             complement(<1..1138)
                     /locus_tag="PKG74_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014682317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOB90001.1"
                     /translation="MLNLQLVADNFVSPVKLVESPDDTGRLFVADRIGVIKIIKNKKV
                     LDEPFLDLKANLVELQENYDERGLLGMAFSPNFETNRKFYVYYSAPLRGGAPAGFDHT
                     SRISEFTVSESNPDKADLNSEKIIMEIDQPQFNHNGGEIIFGPDGYLYVGVGDGGNAD
                     DFGLGHPAMGNGQDLKSPLGKILRIDANGGVPSDNPFHNAIYAYGFRNPFRMSFDRKT
                     GELFVGDVGQNLWEEVDIVEKGKNYGWNIKEGNHCFSHESPDESPADCRNTGYNGEEL
                     VAPILEYSHTEGISLIGGFVYRGEKIPALYGKYVFGDWSGSFTSAAGKIFVAEPPAQG
                     ESDGWSITQTKEIDSFVLGFGEDKNGELYLLTSGEAGPAGNAGKI"
     gene            complement(1225..1593)
                     /locus_tag="PKG74_01560"
     CDS             complement(1225..1593)
                     /locus_tag="PKG74_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HOB90002.1"
                     /translation="MKKSIRIAITIVAVAVIGIAFYYLLTTQRSGSEEIQQPQGENAI
                     FIENFAYSPKELTISVGETVIWTNRDSVRHNVVSDSGNELAADLLGQNESYTHTFDRA
                     GEYPYHCAPHPFMTGKIVVQ"
     gene            complement(1598..1978)
                     /locus_tag="PKG74_01565"
     CDS             complement(1598..1978)
                     /locus_tag="PKG74_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90003.1"
                     /translation="MDTNYRQEGKRKIFVNTLLWGFGLWFFGYILGIAFFYLVPSNMI
                     GWFVLPLGIVASLWVLLKRIKRESFKDYVNLGLIWTAMAMVLDYIFIVRLFNSTDYYK
                     PDVYLYYALTLLLPVIVGWRKKTK"
     gene            complement(2345..2746)
                     /locus_tag="PKG74_01570"
     CDS             complement(2345..2746)
                     /locus_tag="PKG74_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90004.1"
                     /translation="MIRYIIASIVGGFLFGTMDGVINANSYAKKLFEVYKPIAKTSIS
                     APAGIIIDLIYGFIMAGLFLLLYKSLPGETGLLKGLSFAGIAWFFRVVMSVVSSWMTF
                     NVPVSTLGYSLVTGLVEMLVLGILFGLTLKP"
     gene            complement(2882..2971)
                     /locus_tag="PKG74_01575"
     tRNA            complement(2882..2971)
                     /locus_tag="PKG74_01575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2935..2937),aa:Ser,seq:cga)
     gene            complement(2979..3051)
                     /locus_tag="PKG74_01580"
     tRNA            complement(2979..3051)
                     /locus_tag="PKG74_01580"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3016..3018),aa:Ala,seq:tgc)
     gene            complement(3109..3181)
                     /locus_tag="PKG74_01585"
     tRNA            complement(3109..3181)
                     /locus_tag="PKG74_01585"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3146..3148),aa:Ala,seq:ggc)
     gene            3276..3362
                     /locus_tag="PKG74_01590"
     tRNA            3276..3362
                     /locus_tag="PKG74_01590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3310..3312,aa:Ser,seq:tga)
     gene            3401..3739
                     /locus_tag="PKG74_01595"
     CDS             3401..3739
                     /locus_tag="PKG74_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOB90005.1"
                     /translation="MVTLKRIYEPYGKGDGFRILVDRLWPRGVSKEKAKINLWLKDIG
                     PSNELRKWFNHEVEKWPEFKKRYKQELKSKKELVEELRSAHRKHKTITLLYAAKDEKR
                     NEAIIIKDLL"
     gene            complement(3784..>3990)
                     /locus_tag="PKG74_01600"
     CDS             complement(3784..>3990)
                     /locus_tag="PKG74_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90006.1"
                     /translation="SYVNIQASTQTPINGSFTCVCNNGSIISGSLASGVRCQYVGNVE
                     TCVSRCSSYGGWRGGTNDFTCVNY"
BASE COUNT         1133 a          977 c          806 g         1074 t
ORIGIN      
        1 atatcttccc cgcattcccc gcggggccgg cttccccgga cgtaagaaga tacaattctc
       61 catttttgtc ttcaccaaat cccaagacaa aagaatcgat ctcttttgtc tgggttatgc
      121 tccagccgtc gctttctcct tgagccggcg gttcggccac gaatattttt ccggcggcac
      181 tggtgaagct tccgctccaa tctccgaaaa catactttcc gtacaaggcg ggaatctttt
      241 ctcccctata gacaaagccg cctataagag atatgccctc cgtatggctg tattccaaaa
      301 tgggagccac caattcttct ccgttgtagc cggtattccg gcaatcggcc ggcgattcat
      361 cgggactttc gtggctgaag cagtgatttc cttctttgat gttccagccg taattttttc
      421 ctttctcgac aatatcgact tcttcccaca gattctgccc cacatcgcct acgaacaatt
      481 ctccggtctt tctgtcgaat gacatcctga acggattcct gaatccatag gcgtaaatcg
      541 cgttatggaa aggattgtcc gaaggaactc ctccgttcgc gtcgattcgc agaattttcc
      601 ctaaaggact tttaaggtct tgaccattac ccatggccgg atgtcccaaa ccgaaatcgt
      661 ccgcgttccc gccatcaccg acgccgacat agagatagcc gtccggcccg aagattattt
      721 ctccgccgtt gtggttaaat tgcggctgat cgatctccat aatgattttt tccgagttca
      781 aatccgcctt gtccggattg ctttcggaaa ccgtgaattc cgagatcctg ctcgtatgat
      841 cgaatcccgc gggagcgcct ccccggagcg gagcgctgta ataaacataa aatttcctgt
      901 tggtttcgaa atttgggctg aaagccattc ccaaaagtcc gcgttcgtca taattctcct
      961 gcagctccac cagatttgcc ttcaaatcca aaaacggttc atccaaaacc tttttgtttt
     1021 tgattatctt gatcacgccg attctgtcgg cgacgaacag ccttcccgtg tcatccggcg
     1081 actcaaccag ttttacggga gaaacgaaat tatcggcgac caattgtaaa ttaagcattc
     1141 ccgttttttt tgaagaattg ataataaaaa ataaaaccac taacgcgacg gcgacaatta
     1201 gaatgttttt gtatttcatg ttttttattg aacaacgatt ttacccgtca taaatggatg
     1261 cggcgcgcag tgatagggat attctcccgc ccgatcgaaa gtatgcgtat agctctcgtt
     1321 ttggccaagt agatccgccg ccaactcatt cccgctgtcg gaaacgacgt tatgtctcac
     1381 cgaatccctg tttgtccaga tgaccgtctc gccgacggaa atcgtcagtt ctttgggaga
     1441 atacgcgaaa ttttctataa aaatagcgtt ttccccttgc ggttgctgta tttcttccga
     1501 gcccgacctt tgcgttgtta gcaaataata aaaagcgatg ccgataacgg cgacggccac
     1561 tatcgtaatc gcgatccgta tggatttttt catgttttta ttttgttttt ttccgccagc
     1621 cgacgataac cggcagaaga agggtcagcg cgtaataaag atagacgtcg ggcttgtaat
     1681 aatcggtcga attgaaaagt cttacgataa agatataatc caagaccatg gccatcgctg
     1741 tccaaataag gccgaggttc acataatctt taaacgattc ccgtttgatt ctcttcagca
     1801 atacccaaag gctcgccact attccgagcg gcaaaacaaa ccagccgatc atatttgagg
     1861 gaacgaggta gaaaaaagca atgcccaaga tatatccgaa aaaccacaga ccaaatcccc
     1921 acaacaacgt atttacaaaa atttttcttt tgccttcctg tcggtaattt gtatccatga
     1981 tttcattata acaaatccgc cctcgaatct ccgatatttc tcaaacatat aatttgaaac
     2041 tccttaacag gagcttgttt ttttgaaaat acctaaatca ccgagggttc gtcgagcgcc
     2101 ctcccttttt ttccgcccgc ctcgccttaa actggaacgg gtacgatttt aagtctttct
     2161 ttgaaaatca gcccaaagtg ataacatgcc caaggttaga aaaaggacca cctctatcca
     2221 attctgtttt tgaaacgcct gaaaaagtgt tgctccaaaa aagattataa acaccgtcag
     2281 attaataaat ttatatttcc ccatatttat atcttaacaa ttttattttt ggcggcaaat
     2341 tcttttaagg ttttaatgtt agcccaaata aaatccctag cactaacatt tccaccaaac
     2401 ccgtaacaag cgaataacct aaagtgctta ccgggacatt aaacgtcatc caagaagaaa
     2461 cgaccgacat tacaacacga aagaaccaag caataccggc gaaacttaaa ccttttaata
     2521 aacctgtttc cccgggcaaa ctcttgtaaa gcaacaaaaa caaaccggcc attataaaac
     2581 cataaatgag gtctattatt attcccgcgg gtgcgctgat tgaagttttg gcaattggtt
     2641 tataaacctc aaaaagtttc ttggcgtaag aattggcatt aattacgccg tccatagttc
     2701 cgaacaaaaa tccgccaact atgctggcaa taatgtatct aatcataatt ttgaattggt
     2761 tgtatgtatt attttaacaa ttcttctatt ggtgggtgtt gtatagttta tatccgcttt
     2821 cttggctaaa tcgtcttgtg tcaggccaag ttttgcccgc attttcttat ctttttccaa
     2881 ctggcggagg gtacaggatt tgaacctgtg atcccgtttc cgggaaaccg catttcgagt
     2941 gcggcgcact agaccactat gcgaaccctc catcaaagtg gacccgatcg gactcgaacc
     3001 gatgacctcc acattgcaaa tgtggcgctc taccgactaa gctacgggcc cgaaggatca
     3061 ggatcataat agcgagtttt tacgactcgg acaataagta aattcttgtg gacctaaggg
     3121 gattcgaacc cctcgcctct tctatgccat agaagcgctc taccaaatga gctataggcc
     3181 catcaaaaca atgtctattg taaaggattt tccgggaaaa acaaatataa accgcttgta
     3241 aacaaggttt ctagaatata agctgaaatg gaacaggaag cgtgccagag tggtcgaatg
     3301 ggcctgtctt gaaaacaggt atgcccgaaa gggcatcaga ggttcgaatc ctctcgcttc
     3361 cgccctatgt ttttattcat aaacgaatag aatataagta atggttactt taaaacggat
     3421 ttacgaacca tacggtaaag gcgacggctt ccggatcttg gtcgacagac tttggccgag
     3481 gggcgtgagc aaagaaaagg ccaaaatcaa tctatggctc aaggacatcg ggccgagcaa
     3541 tgaactccgc aaatggttta atcacgaagt ggaaaaatgg cccgagttca agaaaagata
     3601 caagcaagaa ctcaaaagca aaaaagagct cgtggaagag ctgaggagcg cacatcgcaa
     3661 gcacaagacg ataacgctcc tttacgccgc caaagacgaa aaacgcaacg aagcgataat
     3721 catcaaagac ttgctgtaaa ccaaaaaaga ctctccccgc gggagagtct tttgattctc
     3781 gactcaatag ttcacgcaag tgaaatcatt ggttccccct ctccatccgc cgtaggaaga
     3841 acatctactg acgcaagttt ctacattgcc tacatattgg caccgcactc cggaagcaag
     3901 agatccgcta attatgctgc cgttgttgca aacgcaagta aacgaaccat tgatcggagt
     3961 ctgggtcgat gcctgtatat tgacatatga
//
LOCUS       DAOPQT010000044         3835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_25821_length_3835_cov_3.481481, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000044 DAOPQT010000000
VERSION     DAOPQT010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25821_length_3835_cov_3.481481"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..950
                     /locus_tag="PKG74_01605"
     CDS             60..950
                     /locus_tag="PKG74_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOB90007.1"
                     /translation="MARNIYTEKEANVKRTWMLFTVFIAVVIGLGWGFGYFYDNPAIL
                     LGAIVFSVFMSAGSFLFSDNIALRLAGAKPVARRDNPELHRLVENLAIAAGAAQPEIY
                     IVDDPSPNAFATGRSPDHSAIAVTTGLLQKLDRQELEGVLGHELSHIENRDTLIATTA
                     AVLVGFVAILADIFMRLPQRSRRSDDDRELRGVLYLIGLIFAILAPIAVNLIQLAISR
                     KREFLADASSALLTRYPEGLASALQKISNDRTPLRSAGSATAHLWIVNPLKSKEMVSN
                     LFSTHPPIEERIQRLREMSL"
     gene            complement(956..2041)
                     /gene="rsgA"
                     /locus_tag="PKG74_01610"
     CDS             complement(956..2041)
                     /gene="rsgA"
                     /locus_tag="PKG74_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOB90008.1"
                     /translation="MTDETTKIKLEDLGYDEFFDSKYRSSGLEGLSIGRVVAEHKEAY
                     RVKSPNGEYLAKITGKQIFKAVKRADYPAVGDWVAISELDGKKAVIRHILPRKTILKK
                     KYSDKQDAQVIAANIDTAFIVEAMDRDYNLNRLERYLVLTNEAGIEPVIVLNKTDLIP
                     EIELDQRIKEIKNRFGEIDVLRTSAVENRGLDELTNRISKGKTYCFLGSSGVGKSSLI
                     NKLLKKNAIETREISGSTGKGRHTTTTREMYFLESGGIVIDNPGTREVGITEAGTGIE
                     HVFDEIISLSQKCKYVDCAHVREPGCAVLQALREGKLDKAKYENYIKLRKENEFYEMT
                     DADKKKKERRFGKFLKKAIDQLKELES"
     gene            complement(2319..2453)
                     /locus_tag="PKG74_01615"
     CDS             complement(2319..2453)
                     /locus_tag="PKG74_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90009.1"
                     /translation="MSKIDDDEKKPVTPAVEPDTDLQSYEEKGFTPEKKDDGNSDNKK
                     "
     gene            complement(2441..2956)
                     /locus_tag="PKG74_01620"
     CDS             complement(2441..2956)
                     /locus_tag="PKG74_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90010.1"
                     /translation="MSSENLDLIFQEVKDRLKDQLLTADQLATKLNFLLGFNTIVIAA
                     IFPGLFDKVQIDKLDIHPIIIFVIAFFLLSIYFDLKGLKIQKYRRDPDPEQLYKKYIN
                     SEADDTKKVLISNYIASFKENLCKLSPVRKCFRASVILSSIALMLIFIFFAKDSILTI
                     WQILTTTICQK"
     gene            complement(2953..>3835)
                     /locus_tag="PKG74_01625"
     CDS             complement(2953..>3835)
                     /locus_tag="PKG74_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HOB90011.1"
                     /translation="TIIDCGKAWKEVKLEQVVFILKKGKKSASYTSGKFENGNLKILG
                     KINKKDAEDFGFFLNSIFESELSIAKKIKSNSIFLNDVSSNQRGAILQKFISETGDKE
                     VIGGAQTQKFGIIGIKGKIDKNKIDNDQAFIKNNSVLIQNIVAHIENPIDHIKIIACV
                     PERNDYIIVDTINQISLNGDIDKNVIWYLLNSKLMSWYAYRFIFGKAIRTMHFDNSIT
                     ARLPIPKDIKSLQNELAKPINEFLKLGKEFQNTPAKTDKSNNLKIEMEKMEKEIDGVI
                     YKSYGLTIDEIEIIEKI"
BASE COUNT          937 a          848 c          758 g         1292 t
ORIGIN      
        1 ttttaacttt atttaaaacg gaaggcgctt ttttaccatc cgcggattta acgaatacaa
       61 tggccagaaa catttatacg gaaaaagagg cgaacgtgaa aagaacgtgg atgcttttca
      121 cggttttcat tgctgtggtc atcggtctcg gctggggatt cggatatttt tacgacaatc
      181 cggccatact gctcggagcg atcgttttca gcgtcttcat gagcgccggg agttttcttt
      241 tctccgacaa tatcgccctc agacttgctg gcgctaaacc ggtcgcgcgg cgagacaacc
      301 ccgaactcca tcgtctggtg gaaaatttgg ctatcgccgc cggcgcggcc cagccggaaa
      361 tatacatcgt ggacgatccg agccccaatg ctttcgcgac cggccggagc ccggaccatt
      421 cggcgatcgc cgtcaccacc ggcttgcttc aaaaattgga cagacaggaa ttggaagggg
      481 ttttgggcca cgagttgagc cacatagaga accgcgacac tttgatcgcg accacggccg
      541 ccgtgcttgt cggatttgtc gccatactcg cggacatttt catgcgtctg ccccaaagat
      601 cacggaggag cgacgatgat cgggagctcc ggggagttct gtatcttatc ggacttattt
      661 ttgccatact cgcgccgatc gccgtcaacc tgatccaact cgccatttcc cggaaaaggg
      721 aatttctggc cgatgcttca tccgcacttt tgacgcgtta tcccgaaggt ttggcctccg
      781 ccctccaaaa aatctcgaac gatcgaaccc cgcttcgatc ggctggttcg gccacggccc
      841 atctttggat agtcaaccct cttaaatcga aagaaatggt atcgaatctt ttttccaccc
      901 atcctccgat cgaagaaaga atccaaagac tgcgggagat gagcctttga tttttttaag
      961 actcgagttc tttcagctgg tctatcgctt ttttcaggaa tttcccgaac cggcgctctt
     1021 tctttttctt atccgcgtcg gtcatttcgt agaattcgtt ttcttttctc agcttgatgt
     1081 agttttcata tttggctttg tccagttttc cttcccgaag ggcttggagg acagcgcatc
     1141 ccggttcgcg gacatgcgcg caatcgacgt atttacattt ttgggacaag gagattattt
     1201 cgtcgaatac atgttcgatg ccggtgcctg cttccgttat cccgacctct ctcgtgcccg
     1261 gattgtcgat cacgatcccg ccgctttcca aaaaatacat ttctctcgtc gtagtcgtgt
     1321 gtctgccttt gccggtggat ccgcttattt cccttgtttc gatggcattt ttcttcagta
     1381 atttgtttat caaagaagat tttccgactc ccgatgatcc gaggaagcaa tatgttttcc
     1441 ctttggagat gcgattcgtg agctcgtcga gcccgcggtt ctcaacggcg ctcgtccgga
     1501 gaacgtcgat ttcgccgaat ctgtttttga tttcttttat tctttggtcc agttcgattt
     1561 ccggaatcaa atcggtttta ttcaacacga taaccggttc gatgccggct tcgttcgtta
     1621 aaaccagata tctttccaag cggttgagat tataatcccg gtccatcgct tccacgataa
     1681 aggccgtgtc gatattcgca gcgataacct gcgcgtcctg tttgtcgctg tactttttct
     1741 tgagtatggt ttttctcggc aaaatatgac ggatgaccgc ctttttcccg tccaattcgg
     1801 aaatcgccac ccagtcgccg acagcgggat agtccgctct tttcacggct ttaaatatct
     1861 gtttccctgt aatcttggcc agatactcgc cattcgggct tttcaccctg taagcttctt
     1921 tgtgttcggc aacgactcgg ccgatcgata agccttcgag tcccgaagac ctgtatttcg
     1981 aatcgaaaaa ttcatcgtag ccaaggtctt caagtttgat tttggttgtt tcgtcggtca
     2041 ttttgtttcg cggttcaggc gggttttatc gagcaccagg gcctttaatc attgtggcgg
     2101 tgttttttgg acgaagttcg aatatttttt gagcaaaatc ccgaaaacta atttggactc
     2161 ggcggggcaa actatttttc tggggaaata gaattgcctc gcctcggctt tcttcccgcc
     2221 cgcaggagcg aggtctcgag gaggaacgcg ggcgggattt tgaacctttc ctttttcgct
     2281 tccgaatttc gtgccagttg aaaactggcg cgccaccttt actttttatt atcggagtta
     2341 ccgtcgtctt tcttttctgg ggtaaatcct ttttcttcgt aactttgcaa atccgtatca
     2401 ggttctactg cgggagttac gggcttcttc tcgtcatcat ctatttttga catattgttg
     2461 ttgttaatat ttgccatatt gtaagtatac tatctttagc aaaaaatata aatattagca
     2521 ttagtgctat tgatgataat ataaccgaag cacgaaaaca ttttctaaca ggtgataatt
     2581 tacaaaggtt ttctttaaac gatgcaatat aattagaaat aagaactttt tttgtatcat
     2641 ctgcctcaga atttatatat tttttataaa gttgttcggg gtctggatct cttctatatt
     2701 tttggatttt taatcctttt aaatcaaagt aaatagataa caggaaaaaa gcaatgacaa
     2761 aaataataat cggatgaata tccaatttgt ctatttgaac tttgtcaaac aaccctggaa
     2821 atattgcagc tatcacaata gtattaaagc ccaaaagaaa atttaacttt gttgctaatt
     2881 gatccgctgt aagaagctga tcttttaatc gatcttttac ttcttgaaaa attaaatcta
     2941 aattttcgct actcatattt tttcaattat ttcgatttca tcaattgtaa gtccataaga
     3001 tttataaatt accccatcaa tttccttttc cattttttcc atttcaattt tcaaattatt
     3061 tgatttatcg gtttttgctg gcgtattttg aaattctttg ccaagtttta gaaattcatt
     3121 gatcggcttt gctaattcat tttgcaaact tttaatatct tttggaattg gcagacgagc
     3181 tgttattgag ttatcaaaat gcatggtgcg aattgcttta ccaaaaataa agcgataggc
     3241 ataccaactc atcaacttgg aattaagcaa ataccagatc acatttttat caatatcgcc
     3301 attaagagaa atttggttta ttgtgtcaac gataatgtaa tcatttcttt ctggaacaca
     3361 agcgattatt tttatatggt caatcggatt ttcaatatga gctacgatat tttggataag
     3421 aaccgaatta ttttttataa aagcttgatc attatcaatt ttatttttat caatttttcc
     3481 ttttattccg atgattccga atttttgagt ttgcgctccg ccaatgactt ctttatctcc
     3541 tgtttctgaa atgaattttt gcaaaatcgc tcctcgctga ttggagctta catcattcaa
     3601 gaatattgaa tttgatttta tttttttagc aatagataat tccgattcga aaatactatt
     3661 caagaaaaat ccaaaatctt ccgcgtcttt tttgttgatt ttccctaaaa ttttaagatt
     3721 accgttttca aattttcccg aagtataact tgctgatttt ttgccttttt tcaaaataaa
     3781 gacaacctgt tcaagtttaa cttctttcca cgcttttcca caatcaatga ttgtt
//
LOCUS       DAOPQT010000045         3829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_25883_length_3829_cov_3.070217, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000045 DAOPQT010000000
VERSION     DAOPQT010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25883_length_3829_cov_3.070217"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKG74_01630"
     CDS             complement(<1..291)
                     /locus_tag="PKG74_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90012.1"
                     /translation="MILILFVFFAGIVWVIWDFGLFGYLPKYDRAAIDRFVSDYYADY
                     ARSININKNWRDFIEAYAVDNGIELLIAEANGFIKDSLNAKQRHFEIIDILNV"
     gene            complement(353..805)
                     /locus_tag="PKG74_01635"
     CDS             complement(353..805)
                     /locus_tag="PKG74_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90013.1"
                     /translation="MDIRRKIPKGQTMLFMVFTMSIVILGATALANLLVVYELRQASS
                     TSQSTQALFSADAGIERILMGRFGCCASEDGDLCSCFPEANNRDGSLDGQSFQVYIKC
                     SDIRHPPEDCRPARRPEDPLIERWYAVGKDETGQIVRTLEITFTEETR"
     gene            complement(807..1322)
                     /locus_tag="PKG74_01640"
     CDS             complement(807..1322)
                     /locus_tag="PKG74_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90014.1"
                     /translation="MTNFSKKSGFTLVEVTVTLGIFVGVVFMILSIFTNSLKAERNVA
                     NQTAILDNLALVIEQMAREIRTGTNYSTLEGDSSLRFTNYQQQEVTYAKAPDGDYITK
                     KTGSDFPKRLTADDVQVTDLKFDIIQPQLSYYPPRVTIKIQVESSLFKGPLTFQTTVS
                     ERLFYYRPPES"
     gene            complement(1328..1849)
                     /locus_tag="PKG74_01645"
     CDS             complement(1328..1849)
                     /locus_tag="PKG74_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90015.1"
                     /translation="MRSAKGQFIVEGMIAMGIIVSSVLGIFSLAVSALRTSSVVTDQF
                     IAANLAAEGLEVTKNILDSNVVRGAAWNHDFSESKCFEVQYDAADIGNDLKPCDDERA
                     FEKTRFLNIDNNNLYSYDSGEPTIFKRWIVITPISSYKIRATATVAWRDPRHPTTLSS
                     STIATDFVGWRAE"
     gene            complement(1870..2439)
                     /locus_tag="PKG74_01650"
     CDS             complement(1870..2439)
                     /locus_tag="PKG74_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOB90016.1"
                     /translation="MAISGFSTSPKKSAQGFTLVEMVVTIGITSLLLMSISISGSSIK
                     REFTLSKSQEELRSLITYARFLSVATLADPEGGLVCGYGVHIVPGENRAYIFRDQGEG
                     GVCLENAIGLPSDLSSEGSLYSMTLDPLVVFESGEQKISFIPPDPAVAFDPGADEEGG
                     KTIVIRARDGSGTPRQVRVLSSGMVSIVR"
     gene            complement(2408..3253)
                     /locus_tag="PKG74_01655"
     CDS             complement(2408..3253)
                     /locus_tag="PKG74_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOB90017.1"
                     /translation="MIVNSLILFFFGICIGSFLNVVILRYDPDKTALVGKHLGGRSKC
                     RSCGKTLRWFELIPIFSFFVQKGRCRTCHRKISWQYPIVEILSGIILVAAYLKWGLTL
                     KALLIALFALSLLVLSFIDFYFQVIPDELNLFIGLLGVLYVLLGWQTKAFGLTEGSFI
                     GYYASVLGLRQNIFLNHLAAALFGGLLFMGLIFLTRGRGMGMGDMKLGAAAGLFIGWP
                     DIVLALMLSFILGGFFGGISLFLRRKKMKDMLPFGPFMAAGILLIVFLGFNICNGYFR
                     FFNLA"
     gene            complement(3261..3722)
                     /locus_tag="PKG74_01660"
     CDS             complement(3261..3722)
                     /locus_tag="PKG74_01660"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOB90018.1"
                     /translation="MKSQKGFTLIEMVIVIAVIGILMGLVFSGTQSVQSNARDTKRMA
                     DLKKVQTYLELYYNKCNHYPGAGGASCSADVTSPVSWEQLRTALIPAVADADDIPEED
                     PQGNPYQYGVETPRGFDYVLGATMERGIPAGGIEVATFGIACGQNKLYCLK"
BASE COUNT         1037 a          994 c          828 g          970 t
ORIGIN      
        1 gacgttcaaa atatcgatta tttcgaaatg ccgctgtttc gcgttcaagc tgtccttgat
       61 aaaaccattc gcttccgcga tcaaaagctc tattccattg tcgacggcgt aagcctctat
      121 gaaatcccgc caatttttgt tgatgttgat gctcctcgcg taatcggcgt aataatccga
      181 tacaaaccta tcgatggcgg ccctatcgta tttgggaaga tagccgaaca atccgaaatc
      241 ccagatcacc caaacgattc cggcgaaaaa gacgaataaa atcagaatca agaatattct
      301 ccgttttagc ggagattccg aagatgccga acgtcccgtc ttgcggctca tctcatcttg
      361 tttcttcggt aaaggtaatt tccaaggtgc gcacgatctg accggtttcg tccttgccga
      421 cggcatacca tctttcgatt aaaggatctt ccggccgtcg ggcaggccta caatcctccg
      481 gaggatgcct gatatctgaa cattttatat aaacctggaa gctttgtccg tccaggcttc
      541 catctctgtt gttcgcttcc ggaaaacaag aacaaagatc tccgtcttcc gaagcgcagc
      601 agccgaaccg gcccatcaat atcctttcga tgccggcgtc ggccgaaaaa agggcttggg
      661 tcgactggct ggtgctcgat gcttgccgca gttcgtaaac cacaagcaaa ttggccaaag
      721 ccgtcgctcc gagaatgacg atcgacatgg tgaaaaccat gaaaagcatg gtttgtcctt
      781 tgggtatttt tctccggata tccatattat gattcgggtg gccgataata gaacaacctt
      841 tcgctgaccg ttgtttggaa agtgagcgga cccttgaaca aagagctttc tacctgaatt
      901 ttgatcgtta ccctcggcgg ataataagat agctgcggct ggataatatc gaacttcaaa
      961 tccgtcactt gaacgtcgtc ggcggtcaaa cgtttcggaa aatcgctgcc ggtttttttg
     1021 gtgatgtaat ctccgtccgg cgctttggcg taagtcactt cctgttgctg ataattggtg
     1081 aatctcaatg aactgtcccc ctcaagcgtg ctgtaattcg tacccgttct tatttctcgc
     1141 gccatttgct ctatcactaa agcgagattg tcgaggatgg ccgtctgatt ggccacgttc
     1201 cgttccgctt tcaaggaatt ggtgaaaatg gagaggatca tgaaaacgac tcccacgaaa
     1261 attcccaaag tgacggtgac ttccaccagg gtgaatccgg attttttgga aaaattcgtc
     1321 atatttgtca ttccgcgcgc cagccgacga agtccgtggc tattgtcgac gaactaagcg
     1381 tggtcggatg cctcggatcg cgccaagcaa cggtggccgt cgccctgatt ttataactgg
     1441 atataggcgt gatgacgatc caccttttga aaatcgtcgg ctcgcccgag tcataactgt
     1501 ataagttgtt attatcgata ttcaagaacc tcgttttctc gaaagctctt tcatcatcgc
     1561 aaggctttaa atcgtttccg atatctgccg cgtcatactg tacttcaaag catttggatt
     1621 cggaaaaatc gtgattccaa gcggcccctc tgacgacatt gctgtccaga atgtttttcg
     1681 tcacctctag gccttcggcc gccagattgg cggcgatgaa ttggtcggtg acgaccgaac
     1741 ttgtccgaag ggcgcttacc gccaagctaa aaataccgag aacgctcgaa acgatgatcc
     1801 ccatggcgat cataccctcg acgatgaatt gccctttggc gcttctcata ttgtttgatt
     1861 atagccggtt taacggacta tcgaaaccat acccgaagag agaaccctca cttgccgcgg
     1921 ggttccggat ccgtccctcg ccctgataac tatggtcttt ccgccttcct catcggctcc
     1981 gggatcgaac gccaccgccg gatccggagg aataaacgat attttctgtt ctccactttc
     2041 gaagactaca agcggatcaa gggtcatcga ataaagcgat ccctccgaag acaaatcaga
     2101 cggcaagcca atggcatttt ctaaacacac ccctccttcc ccttgatccc tgaagatata
     2161 agcgcggttt tctccgggga cgatatgcac gccgtacccg cagacaagcc caccctcggg
     2221 atcggccaaa gtggcgacac tcaaaaatct ggcgtaagtg ataagactcc ttaattcttc
     2281 ctgacttttg gaaagggtga attctctttt gatcgaactt cccgaaatgc ttatcgacat
     2341 caagagcaat gaagtgatgc cgatcgtcac caccatttca accaacgtga atccttgcgc
     2401 cgatttttta ggcgaggttg aaaaaccgga aatagccatt acatatatta aagcccaaaa
     2461 aaacgattaa gagaatcccg gctgccatga acggaccgaa gggcagcatg tctttcatct
     2521 tttttctccg taaaaacaga cttattccgc cgaaaaatcc gccgagaata aaagaaagca
     2581 tcaaagctaa aactatatcc ggccagccga taaaaagtcc ggcggccgcg ccgagcttca
     2641 tgtcgcccat acccattccc ctacctcggg ttaagaaaat caatcccata aaaagcaatc
     2701 ctccgaatag cgcggctgcc aagtgattca aaaaaatatt ctgccgcagt cccaaaacgc
     2761 tcgcgtaata tccaataaac gacccttcgg tcaggccgaa ggctttagtc tgccacccga
     2821 gcaaaacgta caatactccc aagagtccga taaagagatt cagctcgtcc ggaatcacct
     2881 ggaaataaaa atcgatgaaa gacagaacaa gaaggcttaa tgcgaaaagc gcgatcaaaa
     2941 gcgctttcaa agtcagcccc catttgagat aagccgcgac gagaataatt ccagataaga
     3001 tctcgacgat cggatattgc caagatattt tcctatggca agttcgacac cttcctttct
     3061 gtacaaaaaa gctgaaaatc ggaataagct cgaaccatcg aagcgtctta ccgcaagaac
     3121 ggcattttga cctgccgccg agatgtttgc cgaccaaagc ggttttatcc ggatcgtatc
     3181 gcaaaatcac gacattcaaa aaactgccga tgcaaatgcc gaaaaaaaac agaatcagag
     3241 aattaacgat cactatcgga ttatttcaag cagtaaagct tattctggcc acaagcaatt
     3301 ccgaaagtgg ccacttctat gccgccggcc gggatacctc tttccatggt cgcccccaaa
     3361 acgtaatcga aaccccttgg agtctccacg ccatattgat aaggatttcc ttgcgggtcc
     3421 tcttcaggaa tatcatccgc atcagctacg gcgggaataa gtgctgttct taattgttcc
     3481 caactaactg gactagtgac gtccgccgaa cagctggcgc cgcccgcccc cggataatga
     3541 ttacatttat tataataaag ctccaaatag gtctggactt tttttaaatc cgccatcctt
     3601 ttggtatctc tcgcattcga ttgaacactc tgtgtgccgc taaagaccaa ccccatcaaa
     3661 atgccgatga ccgcgataac gatcaccatt tcgatcaatg taaaaccttt ttgcgatttc
     3721 atgtttttaa attgatttgc gacttttatt tttatcgttg acccgagttt atgtatttat
     3781 tctacccgaa ttagagcgtg gagacaaact ggaacatcgg caggagaat
//
LOCUS       DAOPQT010000046         3795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_26226_length_3795_cov_2.942513, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000046 DAOPQT010000000
VERSION     DAOPQT010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26226_length_3795_cov_2.942513"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="PKG74_01665"
     CDS             <1..177
                     /locus_tag="PKG74_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOB90019.1"
                     /translation="STLKEFFGLPKPKVLYGGSVDGSNIREIFAQKPIDGVLAGRASI
                     DPGEFAKLVKNAKI"
     gene            180..956
                     /locus_tag="PKG74_01670"
     CDS             180..956
                     /locus_tag="PKG74_01670"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOB90020.1"
                     /translation="MDTKTADKDKAYYKNYKVKMILSLSLVILIFAGVFALTEWGKSL
                     TPARTVTVSADSKVAIAPDLAEVSFSVITEGANPTTLQIENTTKMNAAIQYVKGKGIE
                     AKDVKTSNYNLQPKYEYDRINRTTYISGYTLTQTIIIKIRDLSKIAEIVAGLPGLGVN
                     EISSVRFSIEDPDKFLADTRKEAFDKAFEKAKAMASMNRVSLGKVVGFSESTNNYIRY
                     DYSLEKTAAMGAGVVPAPVPTIEPGSEEITVYVTVIYEIK"
     gene            1203..2270
                     /gene="pgk"
                     /locus_tag="PKG74_01675"
     CDS             1203..2270
                     /gene="pgk"
                     /locus_tag="PKG74_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOB90021.1"
                     /translation="MIAILRINLDIEPDEFKHSLRLRLATEEIKKLLKRYEKIVILSH
                     RDRPAKPDKKLSLKPSKEFLESGIRKKIVFFEKIDFEKIIREINSSSERVFMLENLRF
                     LKGETENDSKLAKSLASLGDIFINDDFASSHRKNASIYGIVDFAKKSVLGGIVKSEIK
                     HLDAIRENPEKPLVLIVGGAKISDKLGVIEKFIGQAEYVITGGGVANTFLKAAGIPVK
                     KSLVENNMVQKARKFLKSGKIVIPADYRERGGEILDIGPKTEKIYRDIILKARTVIWA
                     GPLGYVEDSKFAKGSIAVLKAIAKTKGRAVIGGGDTTALALKHGFRKKNGFLSTGGGA
                     MLEYLAGQRLPGIEIVNRKQS"
     gene            2267..3100
                     /locus_tag="PKG74_01680"
     CDS             2267..3100
                     /locus_tag="PKG74_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOB90022.1"
                     /translation="MNKKIVVLYHKGCSDGFGAAWVLWKKFKDRAIYWPMEYQVPPPA
                     ILKSASEVYMVDFGFLAEIMKTLASKYKITAIDHHQSQKQAIKFAKESVFDIKHSAAV
                     LTWNHLNPKKPTPELLRRIEDVDLWKFKIKDTKEAVAWLESHEQDFKLWDKLIRDFQN
                     PKKRSAFLKEGAAILRYQEQTVKELSDAAVPAVFEGKKAMVVNSPILASQIGNRLASK
                     EGRVAIIWSNKNKKIFVSLRSIGQTDVSELAKRYGGGGHKHAAGFGFKSANNQFPWKT
                     N"
     gene            3085..3489
                     /locus_tag="PKG74_01685"
     CDS             3085..3489
                     /locus_tag="PKG74_01685"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOB90023.1"
                     /translation="MENKLIINCDGGSRGNPGPAAIGAVVGLKEYAEKIGRTTNNVAE
                     WTSLVFAMKKAKQILGKAKAKETVLEIRMDSELVIKQVNGEYKIMNSDLQPLFIDVWN
                     LKQDFKKVEFRHVPREENKRADYLVNLALDSK"
     gene            complement(3516..>3795)
                     /locus_tag="PKG74_01690"
     CDS             complement(3516..>3795)
                     /locus_tag="PKG74_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90024.1"
                     /translation="GSSDSPHSGRGLALHKDLAVSLPSFYPYNGIRPSSLGLGLPSPF
                     GLGRHCSHLLPYGSQLLTGVFPPYQRKESGLSSLFVETEQSSELPIRI"
BASE COUNT         1110 a          877 c          954 g          854 t
ORIGIN      
        1 tcgaccctca aggagttctt cggtctcccg aaaccaaaag ttctttatgg gggatcggtc
       61 gacggctcga atatccgcga gatcttcgcc caaaaaccga tcgacggagt tttggccggc
      121 cgggcaagca tcgatcccgg ggaattcgca aagcttgtta aaaatgctaa aatataaaca
      181 tggatacaaa aacagcagat aaagacaaag cttactataa aaattacaag gtcaagatga
      241 tcttgagctt gtctctggtg attcttatct tcgccggagt tttcgccttg accgaatggg
      301 gcaaaagcct gacgccggcg agaaccgtca ccgtgagcgc cgacagcaaa gtggccatcg
      361 cccctgatct ggcggaggtt tctttctcgg tcatcaccga aggagccaat ccgacaacgc
      421 ttcaaatcga gaatacgacc aagatgaacg ccgccatcca gtacgtcaaa ggaaagggaa
      481 tcgaagccaa ggatgtgaag acttccaatt ataatctcca gcccaaatac gaatacgacc
      541 ggataaatag gacgacctat atcagcggct atactttgac ccagacgatc atcatcaaga
      601 tccgcgatct ctcgaaaatc gcggaaatcg tcgccggtct tccgggactc ggggttaacg
      661 aaatcagctc cgtcaggttc agcatcgaag atccggacaa attcctggcc gatacgagaa
      721 aagaagcctt tgacaaagct ttcgagaaag ccaaagccat ggcttcgatg aatcgcgttt
      781 ctttgggtaa ggtggtcggt ttcagcgaga gcaccaataa ttatatccgc tacgattatt
      841 ccttggagaa aacagcggct atgggcgcgg gagtcgtgcc ggctccagtg ccgacaatcg
      901 agcccggatc tgaagaaatc actgtttacg tcaccgtaat atatgaaatt aagtaagaga
      961 agcaagaata tcttcaacaa aatagtcacc gtcttcattg tcatcgcttt tctcgttccg
     1021 ctcgccttca gataagagaa agctattgac attcggggaa aaagagagta tcatatggct
     1081 agcgagtgca taggctttac gccggtgcgc ccgaaagact atttggaatc ccgcctttta
     1141 ttattgaggc gggatttttt gtcggtttcg tcattgccgg gtttttggat tataattaac
     1201 ccatgatcgc gattttgcgg atcaatctcg atatcgaacc ggacgaattc aagcattctt
     1261 tacgtttgcg gctggccaca gaagaaatca aaaagcttct caagcgctac gaaaaaatcg
     1321 tgattttgag ccaccgcgac cgtcccgcga agccggataa aaagctcagt ctgaaaccgt
     1381 caaaagaatt tctggaatcc gggattcgga aaaagatcgt gtttttcgag aaaatcgatt
     1441 ttgagaaaat tatccgggag ataaactcgt cttccgagcg ggtttttatg ctcgagaatc
     1501 tgcgtttcct gaaaggtgaa accgaaaacg attctaaact cgccaaatcc ttggcttctc
     1561 tgggagacat ttttataaac gatgacttcg cttcgagtca tcggaaaaat gcttcgattt
     1621 acggtattgt cgatttcgcc aagaagtctg ttttgggcgg gatcgtgaag tccgagataa
     1681 agcatctcga cgcgatccgg gaaaatcccg aaaaaccttt ggttttgatc gtcggcggcg
     1741 ccaagatttc ggataaactc ggagttatcg aaaaattcat cggccaagcc gaatacgtga
     1801 ttaccggagg cggtgtggcc aacacttttc ttaaagccgc cgggattccg gtcaaaaaat
     1861 ctctcgtcga aaataacatg gttcaaaaag cccggaaatt cttgaaatcc ggaaaaatcg
     1921 tcattcccgc ggattaccgc gaacgcggcg gcgaaatact cgacatcggt ccgaaaacgg
     1981 aaaaaatata tcgagacatt attttgaaag cacgaaccgt tatctgggcc ggaccgctcg
     2041 gatatgtcga agattccaaa ttcgccaaag gttcgatcgc ggttctgaaa gcaatcgcca
     2101 aaaccaaggg tcgggcggtg atcggcggag gcgacaccac ggctttggca ttgaagcatg
     2161 gtttccggaa gaaaaacggt tttctttcga cgggcggcgg agccatgctc gaatatctgg
     2221 ccggccaacg gcttccggga atagaaatcg tcaatcgaaa acaatcatga acaaaaaaat
     2281 cgtagttttg tatcataaag gttgcagcga tgggtttggc gccgcctggg ttttatggaa
     2341 aaagttcaag gatcgggcga tctattggcc catggaatat caggttcctc cgccggccat
     2401 cttgaaatcc gcttcggagg tttacatggt ggatttcggt tttcttgcgg aaatcatgaa
     2461 aaccctggct tcgaaatata aaataacggc tatcgatcac catcaaagcc aaaaacaggc
     2521 gattaaattc gccaaggagt cggttttcga catcaaacac tctgctgcgg tgctcacctg
     2581 gaatcacctg aatccgaaaa aaccgacgcc cgagcttctg agaagaatcg aagacgtcga
     2641 tctttggaaa ttcaagataa aagacaccaa agaagccgtg gcttggctcg aatcgcacga
     2701 acaagatttc aagctttggg acaaattgat ccgggatttc caaaatccca aaaagcgctc
     2761 ggcatttctc aaggaaggag cggccatact ccgctaccag gaacaaacgg tcaaggaatt
     2821 gagcgacgcg gctgttccgg ctgttttcga gggcaaaaaa gccatggtcg tcaattctcc
     2881 gatactcgct tcgcagatcg gcaaccgatt ggcctcgaaa gaaggccggg tggccattat
     2941 ttggtcaaat aaaaacaaaa agatttttgt ttccttgcgg tcgatcggac agaccgacgt
     3001 ttcggaactc gccaagcgtt acggcggagg aggccacaag cacgccgccg gattcggatt
     3061 caaatccgca aacaatcaat tcccatggaa aacaaactga tcatcaattg cgatggagga
     3121 agtcggggaa accccggacc agccgccatc ggcgcggttg tcgggctcaa agagtacgcc
     3181 gaaaaaatcg gccgaacaac gaacaacgtg gccgaatgga cgagcctggt tttcgccatg
     3241 aaaaaagcca agcagatttt gggcaaggct aaagctaaag agaccgtcct cgaaatacgg
     3301 atggacagcg aattggtgat taaacaagtg aatggcgaat acaagataat gaattctgat
     3361 cttcaaccct tattcatcga tgtttggaac ctgaaacagg atttcaagaa ggtggagttt
     3421 cgtcatgttc cgagagaaga gaacaaaaga gccgattatc tggtgaatct ggctttggat
     3481 agcaaatgat gggttgaccg aaagcccgat ttgtgctata ttctgatcgg taattcagac
     3541 gattgctctg tttcaacaaa cagagaggaa agtccggact ctttccgctg ataaggcgga
     3601 aacacaccag ttaaaagctg gctgccgtaa ggcaagaggt gcgagcagtg acgtcccaag
     3661 ccgaaaggcg agggaagacc caatcccaag ctcgatggtc taattccgtt atagggataa
     3721 aaagacggaa gtgaaacggc taaatccttg tggagtgcaa gaccgcgccc cgaatgggga
     3781 gagtcgcttg agccc
//
LOCUS       DAOPQT010000047         3783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_26321_length_3783_cov_2.879560, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000047 DAOPQT010000000
VERSION     DAOPQT010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26321_length_3783_cov_2.879560"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..439
                     /gene="rplM"
                     /locus_tag="PKG74_01695"
     CDS             95..439
                     /gene="rplM"
                     /locus_tag="PKG74_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOB90025.1"
                     /translation="MEHVIDAKNQKIGRLATKIANILQGKHKAGYNPRLEGEDKVIVK
                     NIGEIELSGRKTEQKVYHRHTGYMGHLKTKTYKQAEAKDPAWALRHAVQGMLPKNSLQ
                     AKRLKRLIIEKQ"
     gene            439..>547
                     /locus_tag="PKG74_01700"
     CDS             439..>547
                     /locus_tag="PKG74_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOB90026.1"
                     /translation="MAKDSKKEGKYFEAVGRRKTAVARVRIMPGETGFTV"
     assembly_gap    548..557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <558..825
                     /gene="rpsI"
                     /locus_tag="PKG74_01705"
     CDS             <558..825
                     /gene="rpsI"
                     /locus_tag="PKG74_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOB90027.1"
                     /translation="DRDRESAYAPLKLISSEKIGVSAKASGSGLPSQAGAVSLGMARA
                     FIKMDAGHRPTLKAAGLLTRDPRMVERKKYGLKKARRAPQWAKR"
     gene            833..1513
                     /locus_tag="PKG74_01710"
     CDS             833..1513
                     /locus_tag="PKG74_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB90028.1"
                     /translation="MKPLFSVIIPTYESGKSLPLALIDTDKFLRESGFEYEIIVADAK
                     SADQTADIVRRFSALIRNLDLVSGETIGHRGKALQTAVQISSGESFIVVDPKTMKIGE
                     ILSKMSSAFMSDKAKIFDVFLPEGAKNKNRLAESLLEIKRGDLFSGVAGFRRGCGNEV
                     FPKIRLSGTGAIWEPVVIAGQAGFKIKTVDMGGIAGEKSFRGNLGPLIRVVYGLRQDV
                     YRLKNKNN"
     gene            1520..1981
                     /gene="greA"
                     /locus_tag="PKG74_01715"
     CDS             1520..1981
                     /gene="greA"
                     /locus_tag="PKG74_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOB90029.1"
                     /translation="MNYYLSAERLEELKKELVELKTVSRIEVSDRIKKAKEYGDLSEN
                     AEYENAREEQDRIESRIEELEDIIKSAVIIKKATQQEVVDIGSTVEVARNGEKKKIVI
                     VGSNETKPEEGLISNESPLGRALLGKKVGDEAVFKNPAGQEIAYLVTKIGE"
     gene            2061..3404
                     /locus_tag="PKG74_01720"
     CDS             2061..3404
                     /locus_tag="PKG74_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOB90030.1"
                     /translation="MLEDIIESRRKKMEMLRREGVNPYPARVKRTMSVAEALTGFSKL
                     SREKKKVYLVGRVISFRDQGGVIFADIDDGTGKIQAVLTKKNLKDFKLLKEVFDAGDF
                     MEVGGILFKTKKGEKSVEAKTCRMAGKSLRPIPSSWFGLDDVEERFRKRYLDIILNED
                     VKKDLVLRSEIVASLRETLHCEGFMEVETPMLQPIPGGALAKPFKTHHNALDTDFYLR
                     VAPELYLKRLLVAGFPKIFEIGRNFRNEGMDREHNPEFTMLELYWAYQDYESLMKFTE
                     KLLKKFIPGKYGRITYSEAMKKYAGRDLMKIPEGKIDEVFKKEVRPKLVNPVFLTGHP
                     KAISPLAKLDEKDPRLVERFQLIVKGTEVANAFSELNDPIDQRERMEDQERRYRAGDE
                     ETTRFDEDFIEAVEYGMPPAAGIGIGIDRLVSLITGKPVKEIIIFPTLRPKKQLQ"
     gene            3406..>3783
                     /locus_tag="PKG74_01725"
     CDS             3406..>3783
                     /locus_tag="PKG74_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOB90031.1"
                     /translation="MIDINLIRENPEKVKKGVERKHYDPGLVDAFLKLDADWRVGKKE
                     IDELRAKQKTFGPESREEAKAIKEQIAAKEEATVGLEKERQAILEQIPNIPADDVPDG
                     KDDTENVVLKQVGDRPEFGFEPKD"
BASE COUNT         1112 a          846 c         1067 g          748 t
ORIGIN      
        1 caggaaagaa aagggggtta tctgcggatc gtgaagctgg gaaccagccg caaaagggac
       61 ggtgcgcctt gggtggaaat aaaattcgtt taatatggaa cacgtgatcg acgcgaaaaa
      121 tcagaaaata ggccgcttgg cgacgaaaat cgccaatatc cttcaaggaa agcacaaggc
      181 cggatacaat ccccgtttgg aaggggaaga caaagtgatc gtcaagaaca tcggcgagat
      241 cgagctttcc ggaagaaaaa ccgaacagaa agtttaccac cgccacacgg gttacatggg
      301 ccacctgaaa accaaaacct acaagcaggc cgaagccaaa gatccggcct gggctttgag
      361 gcacgcggtt cagggtatgc tgcccaagaa ttctttgcag gcgaaaaggt tgaaaaggct
      421 tatcatcgaa aaacaataat ggccaaagac agcaaaaaag aaggaaaata tttcgaggcc
      481 gtgggccgga gaaaaacggc cgtggcccgc gtgaggatca tgccgggaga aacgggtttt
      541 acggtgannn nnnnnnncga ccgcgaccgc gaaagcgctt acgctccgct caaattgatt
      601 tcttcggaaa agatcggtgt gagcgccaag gcgtccggaa gcggattgcc gagccaagcc
      661 ggagccgttt ctctgggaat ggccagggcg tttatcaaaa tggatgcggg acatcggccg
      721 accctcaaag ccgccgggct tttgacaagg gatccgcgca tggttgaaag gaagaaatac
      781 ggcttgaaga aagccaggcg cgctccgcag tgggccaagc gctaaaccta aaatgaagcc
      841 actcttttcc gtcatcattc cgacttacga gagcggcaaa tctttgccac tggctttgat
      901 cgacacggac aaattcttgc gggaatccgg attcgaatac gaaattatcg tggccgacgc
      961 caagtcggcc gaccagacgg cggacatagt ccgccgtttt tcagctctta tcaggaactt
     1021 ggatttggtt tccggggaaa caatcgggca tcgtggaaaa gctttgcaga cggctgttca
     1081 aatatcttcc ggagaatctt ttattgtcgt cgatccgaag acaatgaaaa tcggagaaat
     1141 tttgtccaag atgtcgtcgg cgtttatgtc cgacaaagcg aagattttcg acgttttcct
     1201 tcccgagggt gccaaaaaca aaaaccgatt ggcggagtcg ctcttggaaa tcaagcgcgg
     1261 ggatcttttt tccggagtcg ccggattccg ccgcggatgc ggaaacgagg tgtttccgaa
     1321 gatcaggctt tccggcaccg gagctatttg ggagccggtt gtgatcgccg gacaagccgg
     1381 ttttaaaata aagaccgtgg atatgggcgg aatcgccgga gaaaaaagct tccgaggcaa
     1441 tctcggtccg cttatccggg tagtctacgg tttgcgtcag gacgtttatc gtttgaaaaa
     1501 taaaaataat taaaaacaaa tgaattatta tttgtcggcc gaaaggctcg aggagttgaa
     1561 gaaagagttg gtcgaattaa aaaccgtgag caggatcgaa gtttccgaca ggataaagaa
     1621 agccaaggaa tacggggatc tttcggaaaa cgccgaatac gagaacgcga gagaggagca
     1681 agaccggata gaaagccgca tcgaagagct cgaagatatt attaaaagtg ccgtcatcat
     1741 caagaaagcc acccaacagg aagtggtcga catcggttcg accgtcgaag tggcgcggaa
     1801 cggagaaaag aaaaagatag tcatcgtcgg ctccaacgaa accaagccgg aagagggatt
     1861 gatttccaac gagtctccgc tcggccgggc gctcctcggc aagaaagtcg gggatgaggc
     1921 cgtgttcaag aatccggccg gacaggaaat cgcctatctg gtgacgaaaa tcggagaata
     1981 agaagttttt gaatcctccc cgcaaaaagc gggggaattt gtaaataaaa ggaaatggtt
     2041 taagattatt caatagaacc atgctggaag acatcatcga atcgcgtagg aaaaaaatgg
     2101 agatgttgcg ccgggaaggc gtcaatcctt atccggcgag ggtaaaaagg accatgtccg
     2161 tggccgaagc tttgactgga ttttcgaagc tcagccggga gaagaagaaa gtttatttgg
     2221 tcgggagggt gatctctttt cgcgatcagg gaggcgttat tttcgccgat atcgatgacg
     2281 gcaccgggaa aatccaggct gttttgacga aaaagaactt gaaagatttc aagcttttga
     2341 aagaagtctt cgatgccgga gatttcatgg aagtcggcgg aattcttttc aaaacgaaaa
     2401 aaggcgagaa aagcgtcgaa gccaaaacct gccgcatggc cggaaaatcg ttgcggccga
     2461 ttccttcgag ctggttcggg ttggacgacg tcgaggagag gttccgaaag cggtatctcg
     2521 acatcattct gaacgaagac gtcaaaaaag atttggtttt gcgttcggaa atcgtggctt
     2581 cgcttcggga gacgctacat tgcgagggat tcatggaagt ggaaaccccg atgctccagc
     2641 cgattccggg cggagcgctc gctaagccct tcaaaacgca ccataacgct ttggatacgg
     2701 atttttattt gcgggtggcg ccggaactgt acttgaagag actgctcgtc gccggattcc
     2761 ccaagatttt cgagatcggc cggaattttc gcaacgaagg catggaccgc gagcataatc
     2821 cggaattcac catgcttgag ctttattggg cttaccagga ttacgaaagc ctgatgaaat
     2881 tcaccgaaaa gcttttgaaa aaattcattc cgggaaaata cggacggatc acctacagcg
     2941 aggcgatgaa aaaatacgcc ggccgcgacc tgatgaaaat tcccgaaggc aagatcgacg
     3001 aggttttcaa gaaagaagtg cggccgaaac tggtgaatcc ggtatttctg accggacacc
     3061 cgaaagccat ttcgccgctt gccaaactcg acgaaaaaga tccgcggctt gtcgaaaggt
     3121 tccaacttat cgtcaaagga accgaggtgg cgaacgcttt ttccgaactg aacgatccga
     3181 tcgaccagcg cgaacgtatg gaagatcagg aacgacgtta tcgcgccgga gacgaagaaa
     3241 cgacgaggtt cgacgaggat ttcatcgaag cggtggaata cggcatgccg ccggcggccg
     3301 gcatcggcat cggcatcgac cggctggtgt ctttgatcac cggcaaaccc gtgaaagaaa
     3361 taataatttt cccgacctta aggccgaaaa aacagcttca ataaaatgat cgacatcaat
     3421 ctcatcaggg agaatcccga aaaagtcaaa aaaggcgtgg agcgcaagca ttatgatccc
     3481 gggctggtgg acgctttctt gaaactcgac gccgattggc gagtcggaaa aaaagaaatc
     3541 gacgagcttc gggccaaaca aaagactttc ggaccggaat cgagagaaga ggccaaagcc
     3601 atcaaagagc aaatcgcggc caaagaggag gcgactgttg gtttggaaaa agaaagacag
     3661 gctattttgg agcagatccc gaacattccg gcggacgatg tcccggatgg caaagacgac
     3721 accgaaaacg tggtcttgaa gcaagtcggc gacagaccgg aattcggttt cgagcctaag
     3781 gat
//
LOCUS       DAOPQT010000048         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_28242_length_3582_cov_3.445988, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000048 DAOPQT010000000
VERSION     DAOPQT010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28242_length_3582_cov_3.445988"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="PKG74_01730"
     CDS             complement(<1..1341)
                     /locus_tag="PKG74_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOB90032.1"
                     /translation="MNIPAEVSAIIERLEKAGFKAYVVGGSVRDFLLGKKPKDWDVAT
                     DAAPEEIQEIFKESVYENTFGTVGVKTGSEDPDLKVIEVTTFRIEGKYSDKRHPDKVK
                     FAKTIEEDLARRDFTINAMAMSAAKGKANGIIDPFEGEIDLKKKIIRTVGDPDKRFEE
                     DALRLMRAVRFSAELGFEIETKTFSAMKKNADLLESVSEERIRDEFEKIIMSERAAQG
                     VELLEAAGLLRHILPELREGIGVGQNKHHIYSIWEHSIKALDYAAKKNYSLEVRLAAL
                     LHDIGKPDTKRGDGPDSTFYNHEMVGAKIAKKIMERLKFPKKTSDKVVHLVRHHLFYY
                     NVDEVTPAGVRRFIARVGEEHIDDLLKLREADRIGSGVPKAVPYKTRHLLFMIDKVKR
                     DPISPKMIKLNGSDLMDSLRIEPGPRIGWILNILLEEVLDNPELNVKSKLEKRAE"
     gene            complement(1397..1472)
                     /locus_tag="PKG74_01735"
     tRNA            complement(1397..1472)
                     /locus_tag="PKG74_01735"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1436..1438),aa:Glu,seq:ttc)
     gene            complement(1520..>3582)
                     /gene="recG"
                     /locus_tag="PKG74_01740"
     CDS             complement(1520..>3582)
                     /gene="recG"
                     /locus_tag="PKG74_01740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOB90033.1"
                     /translation="PVRYDDWSHLTDIKDLRPGEYAVIRARVGKIGFRKSWVQKLAIV
                     EAILEDESGSVRTIWFGQPFIKRVLSEGKQAFFSGKVLAGKKGIFLSNPAYETVSDEE
                     NINGLFPKRELLAIYPETKGLTSKGIRFLINKVLPDLEKVPDFIPEEILRKNDFPEIN
                     RALRDVHIPKSSAEANAAKERFAFEELFLLQLGNLLEKKRLAKTKAPSIEFNLDFVKW
                     ALDKLPYGLTFSQKRSLYEVLKDIERHQPMNRLLQGDVGSGKTVIALIAAMETAESGY
                     QAVFMAPTEILANQHYQTFKKLFSDFGHPIGLMTAKSKLAFYGDNLESEMKKKDLFQE
                     VENGRIKILIGTHALIAKSSGENRIRFNNLGLIVVDEQHRFGVRQRAELVKNQARKSP
                     GLTPHFLSMSATPIPRTLAITIFGDLDLSTIDELPQGRKPIITKVVDPLNRDKAYAFI
                     RGEIRKGRQAFVVCPRIESEEDTDYEYSEPVKKDARKSGWSETKTVKKEYERLSKKVF
                     PDLKVAMIHGKMKAEEKQKTMEDFKTRRSDILVSTSVIEVGVDIPNATIMMIEGAERF
                     GLAQLYQFRGRVGRGEHQSFCFLFTESLNETTHQRLKALLEAKNGFELAEKDLEIRGP
                     GALLGAEQTGLPDTTMRALRNPAMIKLGRQSAEQIIAKDPTLRSYPALKDRLEKFKEN
                     IHLE"
BASE COUNT          774 a          927 c          872 g         1009 t
ORIGIN      
        1 ttcggcgcgt ttttcgagct tggatttgac gtttaattcc ggattgtcca aaacttcttc
       61 gagaagaatg tttaaaatcc atccgatccg cggtccgggc tcgatccgga gcgaatccat
      121 cagatcgctt ccgttcaatt tgatcatctt cggggaaatc gggtcccgct tcaccttgtc
      181 gatcataaaa agcagatgtc tcgttttgta gggcaccgcc ttcggcactc ccgaaccgat
      241 gcggtcggct tcgcgcagtt tcagaagatc gtcgatatgc tcttcgccga cgcgagcgat
      301 aaaccttctc actccggccg gagtcacttc gtcgacgttg taataaaaaa gatgatgtcg
      361 caccaaatgg acgactttgt cgctcgtctt tttcggaaac ttcagccgct ccatgatttt
      421 tttggcgatt ttcgcgccaa ccatttcgtg attgtaaaaa gtggaatccg ggccatcgcc
      481 tcttttggtg tccggtttgc cgatatcgtg tagtaaagcc gcgagccgta cttccagaga
      541 ataatttttc ttggcggcgt aatcgagcgc ctttatcgag tgctcccaga tggaatagat
      601 atggtgcttg ttctgaccaa caccgatgcc ttccctgagt tccggaagaa tatgccgcag
      661 aagtccggcc gcctcgagca gttccacacc ttgggctgct cgttcggaca taatgatttt
      721 ttcgaattcg tcgcgaatcc tctcttccga aaccgattcg agcaaatcgg cgttcttttt
      781 catggcggag aaagttttgg tttcaatctc gaatccgagt tccgcggaaa acctgacggc
      841 ccgcatcaga cgcaaagcgt cttcctcgaa acgcttgtcc ggatcgccca cggtccggat
      901 gattttcttt ttcagatcaa tctctccttc gaaaggatcg atgatgccgt ttgccttgcc
      961 ttttgccgcg gacatggcca tggcgtttat ggtgaaatcg cgccgcgcca gatcttcctc
     1021 gatcgttttg gcgaatttaa ccttatccgg atgccttttg tccgaatatt tcccttctat
     1081 ccggaaagtg gttacctcga tgactttcaa atccggatct tcggatccgg ttttgacgcc
     1141 gacggtcccg aaagtattct cgtaaacgct ctccttgaat atctcctgga tctcttcagg
     1201 cgcggcgtcg gtcgccacgt cccaatcctt cggctttttg ccgagcaaaa aatcgcggac
     1261 cgaaccgccg acgacatagg ctttgaatcc ggctttttcg agcctttcga tgatggcgct
     1321 gacttccgcc ggtatattca tgattttctt attcttaata ataccctatt ccggggagag
     1381 aacgataaga attctttggc gctcccaccg ggaatcgaac ccgggtttcc aggatgaaaa
     1441 cctgatgtcc taggccacta gacgatagga gcgtgatctc atactgccaa aaaaaacaag
     1501 agttggcaat aaatccgcgt cattccaaat ggatgttctc tttgaatttt tccagccggt
     1561 ctttcaatgc cggataagat ctcaaggtcg gatctttcgc tatgatttgt tccgccgatt
     1621 gccggccgag cttgatcatc gcgggattac gcaaggctct catggtcgtg tccggaagcc
     1681 cggtctgttc ggctccgagc agggcgcccg gacccctgat ctccaaatct ttttccgcca
     1741 attcgaatcc gtttttggct tcgagcaggg ctttcagtcg ttggtgggtt gtttcgttta
     1801 aagactccgt gaaaagaaaa cagaacgact gatgttcgcc gcggccgacc cttccccgga
     1861 attggtagag ctgggccagg ccgaatcttt cggcgccttc gatcatcatg atcgtggcgt
     1921 tcgggatgtc cacgccgacc tcgatgaccg acgtggaaac gagaatatcc gatcttctcg
     1981 ttttgaaatc ttccatcgtt ttctgttttt cttcggcctt cattttcccg tgtatcatgg
     2041 ccaccttaag atcgggaaaa acttttttgg aaagcctttc gtattctttt ttgacggtct
     2101 tcgtctccga ccatccggat ttgcgggcgt ctttttttac gggttccgag tattcgtagt
     2161 cagtatcttc ttcggattct atccgcggac agacgacaaa agcctggcgt ccttttctga
     2221 tctcgccccg gataaaagca taagctttgt cccggttcag cgggtcgacc actttggtga
     2281 ttatcggttt tctgccttgc ggcaattcgt caatcgtcga cagatccaaa tcgccgaaaa
     2341 tagtgatcgc caaagtgcgg ggaatgggcg tcgccgacat gctcaaaaag tgcggagtga
     2401 ggccgggaga tttccgcgcc tgattcttaa caagctcggc tctctggcga acgccgaagc
     2461 gatgctgttc gtcgacgacg atcaatccca aattgttgaa tctgattctg ttctctccag
     2521 aagatttggc gatcaaagca tgagtaccga tcaggatttt gattcttccg ttttccactt
     2581 cctggaacaa atcttttttc ttcatttccg attccagatt gtcgccgtaa aaagccagtt
     2641 ttgatttggc ggtcatgagc ccgatcgggt gcccgaaatc ggaaaacagt ttcttgaaag
     2701 tctggtaatg ctgattggcc agaatttccg tcggcgccat aaaaaccgcc tgataaccgc
     2761 tttccgcggt ttccatggcg gcgatcaggg cgatgactgt ttttccggaa ccaacgtctc
     2821 cctggagcag ccgattcatc ggctggtggc gttcgatatc cttcaaaact tcgtaaaggc
     2881 ttcttttttg ggaaaaggtc agaccataag gaagcttgtc gagcgcccac ttcacgaaat
     2941 caagattgaa ttcgatggac ggcgccttgg tcttcgccag tctttttttc tcgagcagat
     3001 tgccgagctg gagtaagaat agctcctcga aagcgaaacg ttctttggcg gcattcgcct
     3061 cggccgagct tttagggata tggacatccc gcaaagctcg gttgatttcc ggaaaatcgt
     3121 ttttccgcag gatctcttcc gggataaaat ccggaacttt ttcgagatcg ggtaagactt
     3181 tgttgatcaa aaacctgatg cctttggaag taaggccttt tgtttccgga taaatggcga
     3241 gaagctcacg tttcggaaaa agcccgttga tgttttcttc gtcggaaacg gtttcgtaag
     3301 cgggattgga aaggaaaatc ccttttttgc cggccaaaac ttttccggag aaaaaagcct
     3361 gtttcccttc gctcaaaact cttttgatga aaggctgacc aaaccagatc gtccggacac
     3421 tccccgattc gtcttcgaga atggcttcga caatggcgag tttctgaacc cagcttttgc
     3481 gaaagccgat tttcccgact ctggcgcgga tgaccgcgta ttcgcccgga cgcagatctt
     3541 taatgtcggt gagatgactc caatcatcgt acctgaccgg aa
//
LOCUS       DAOPQT010000049         3535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_28735_length_3535_cov_2.236782, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000049 DAOPQT010000000
VERSION     DAOPQT010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28735_length_3535_cov_2.236782"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKG74_01745"
     CDS             <1..218
                     /locus_tag="PKG74_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90034.1"
                     /translation="SRRRRGRTIIATRDMMNVFNLVKKDVDAATNAVDKSKTASPPEV
                     KYHLEKIKKDIDKMAKYLVQGVEEIGK"
     gene            256..627
                     /locus_tag="PKG74_01750"
     CDS             256..627
                     /locus_tag="PKG74_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOB90035.1"
                     /translation="MKILIIEDEEALARVIKEKFDKEGYDSVIVEEGEEALPKAKQFK
                     PDVILLDLLLPKRNGFEILQDLKMDAELKTAPVIVLSNLGEDENIKRAILLGAADYFV
                     KTQHPISEVIEKVRYVAERGR"
     gene            637..2556
                     /locus_tag="PKG74_01755"
     CDS             637..2556
                     /locus_tag="PKG74_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOB90036.1"
                     /translation="MNKRSDQKLGKKGGGKISRRRFSVERMTEKSGLGDLYRKVFASV
                     SDIVVVFDTDLKLLTMNPAGVKVVRSLGVKHVSVGKRLSEALPFVPKATEAEYRKLIK
                     TGKSSVGEVIYRAGNVARIGYTKRIPIKDKGCVIGILTVIQNITELKKGELALQKNVR
                     RFNAITSLAREMIWESDLPGTITYVSSASKKIMGYYPKEIIGEKLYSRLFFPDNHEET
                     KKLIEGVFSKRKEFHLLKLSAKNKNGKPAVLEVNGTPFFDEEGKISGYIGTAVDVTER
                     ETYEENLKKFELAVENASDHIIITDPKGVILFANKAVSSTTGYSPKEIIGHRSSLWGK
                     QMPPEFYENMWRKIRDEKEVYVGELTNKRKNGELYIAEIRISPVLDENGKIRFYVGIE
                     RDITEAKAIDRAKTEFVSLASHQLRTPLSIIDWYTESLLSGDKGVLTEAQKDYIDEIH
                     KSNRRMVDLVESLLNATRIDMGRLVISPKPVSLPKIADIVLSELAPQIKEKEISVSKI
                     YDDIPEIRLDPDLTRVIFQNLLSNAVKYTPKNGKISLKICRENGNIVIYVADTGIGIP
                     EKQQSKIFDKLFRADNARIANPEGNGLGLYITKAIIEAAKGTIGFESKEGKGTTFVVT
                     FPVSRAKKPQVKKIA"
     gene            complement(2559..>3535)
                     /locus_tag="PKG74_01760"
     CDS             complement(2559..>3535)
                     /locus_tag="PKG74_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOB90037.1"
                     /translation="AQSLDNDDLGRLKAELVGVKGVAGELSGRYQVIANGNNTNTSVI
                     GTEPEYAIVKNLEVAQGDFISEQNLRNASRVAVLGPTVVEDLFGEGADPIGETVKING
                     LKFKVIGVSKSKGGLMNQDDTIFIPLTTIQRSLVGRNRLSTIDIAAESTAVMSFVEEQ
                     TKAILLDEHDIANPDEADFQVMNQAEIVEAVSNITATLTLLLAAIAGISLVVGGIGIM
                     NMMLTTVTERTREIGLRKALGAKRIDVNRQFLFEAIVLTLLGGIIGISLGALISFAIS
                     YSGLIQTKISIPSVLLAFGISAMIGIVFGYYPAKRASALNPIEALRYE"
BASE COUNT          994 a          852 c          910 g          779 t
ORIGIN      
        1 ttagccgccg tcggagggga agaaccatca tcgccacccg cgacatgatg aatgtcttca
       61 atctagtgaa gaaagacgtc gatgccgcca ccaacgccgt cgacaagtcg aaaaccgcgt
      121 cgccgccgga agtcaaatat catctcgaga agatcaaaaa ggatatagac aaaatggcca
      181 aatatttggt ccagggagtc gaagaaatag gcaaatagga agattaaagg tcaaaagtca
      241 aaataataaa aaaacatgaa aatattgata atcgaagacg aagaagcttt ggcgagagtc
      301 atcaaggaaa agttcgacaa ggaaggatac gattccgtta tcgtggaaga aggggaggaa
      361 gcccttccga aagcgaagca attcaagccg gatgttattt tgctcgatct tttgcttccc
      421 aagaggaacg gattcgagat cctgcaggat ttgaaaatgg atgcggagct taaaaccgcg
      481 ccggtgatcg tcttgtccaa tctgggcgaa gacgagaaca tcaaacgggc catacttttg
      541 ggagccgccg attatttcgt caaaacccag catccgataa gcgaggttat cgaaaaagtg
      601 cgatacgtgg ccgaaagggg cagataatgt tttcggatga ataaacgttc cgaccaaaag
      661 ctcgggaaaa aaggcggcgg gaagatttcc cggcgtcgtt tttctgtcga acggatgacg
      721 gaaaaatccg gattgggcga tctttaccgc aaggtctttg cctcggtgtc ggatatcgtc
      781 gttgttttcg acacggatct gaagcttctc acgatgaatc cggccggagt caaagtggtg
      841 cgctccttag gggtgaaaca cgtctccgta ggcaaaaggc tttccgaggc tttgcctttt
      901 gttcccaaag ccacggaagc cgaatacaga aaactgatca aaaccggcaa atcgtccgtg
      961 ggagaagtga tatatcgggc gggtaacgtc gcgcgcatcg gctacaccaa aaggattccc
     1021 atcaaagaca agggttgcgt tatcggaata ctcaccgtta tccagaacat caccgaactg
     1081 aagaaagggg aattagcgct tcaaaagaac gtccggcgtt tcaacgccat cacttctctc
     1141 gccagggaaa tgatctggga aagcgatctt ccgggaacga tcacttatgt gagttcggcc
     1201 tccaaaaaaa taatgggata ttatcccaaa gaaattatcg gagaaaaact ttattcaaga
     1261 ttgtttttcc cggacaatca cgaagaaacg aaaaagctta tcgaaggcgt tttctccaaa
     1321 agaaaggaat tccatcttct gaaactgtca gccaagaaca agaatggtaa accggccgtt
     1381 ctcgaagtaa acggcacgcc gtttttcgac gaggaaggaa agatttccgg ttatatcgga
     1441 acggcggtcg acgtcaccga gcgcgagacc tacgaggaga acttgaagaa attcgaactg
     1501 gccgtcgaaa atgcctcgga ccacatcatc atcaccgacc cgaagggtgt tattcttttc
     1561 gcgaacaaag ccgtttcctc gaccaccggt tattccccga aagaaataat cggtcatagg
     1621 tcgtcccttt ggggaaagca gatgccgcca gaattctacg aaaacatgtg gcgaaaaata
     1681 agggacgaaa aagaagtcta tgtcggcgaa ctcaccaata aaaggaaaaa cggggagttg
     1741 tatatcgccg aaatccggat atcgccggtc ttggacgaaa acggtaaaat ccgtttttac
     1801 gtcggcatcg aacgggatat caccgaagcc aaggccatcg accgcgccaa aaccgagttc
     1861 gtttctttgg cctcccacca gcttcggaca cctctttcca tcatcgattg gtacaccgaa
     1921 tcccttttgt ccggagacaa aggggttttg accgaagccc aaaaagacta tatcgacgaa
     1981 atccacaaaa gcaaccgccg gatggtggat ctcgtggaat cgcttttgaa cgccacgaga
     2041 atcgacatgg ggagactcgt catttccccg aagccggtca gtctgccaaa aatagcggac
     2101 atcgttcttt ccgaactcgc gccgcaaatc aaagaaaaag agatttccgt ttccaagatt
     2161 tacgacgata ttccggaaat aaggctcgat ccggacttga cgcgggtcat tttccaaaat
     2221 cttttgtcga acgccgtcaa atacaccccg aaaaacggaa aaatatccct aaagatctgc
     2281 cgggaaaacg gaaacatcgt tatttacgtt gcggacaccg gcatcggcat tccggaaaaa
     2341 cagcaatcga agattttcga taagcttttc cgggcggaca acgcgaggat cgccaatccg
     2401 gaaggaaacg gccttggact ttacatcacc aaagccatta tcgaggcggc caagggaaca
     2461 atcggtttcg agtccaaaga aggcaaagga accacttttg tggtgacctt tccggtcagc
     2521 cgcgccaaaa aacctcaagt aaagaaaatc gcctgagatt attcgtagcg caaagcctct
     2581 atcgggttta aagcgctggc gcgcttggcc ggataatatc cgaaaacaat gccgatcatg
     2641 gccgagatcc cgaaagcgag tagaactgaa gggatcgata ttttcgtctg gattagcccg
     2701 gaataggaaa tggcgaaaga aatcaaagct ccgaggctga tgccgattat tcctccgaga
     2761 agagtgagaa cgattgcttc gaaaaggaat tgccggttga cgtcgattct tttggcgccc
     2821 agggccttgc gaagccctat ttcccgggtt ctttcggtga cggtcgtgag catcatgttc
     2881 atgatgccga tgccgccgac aacgagcgat atgcccgcga tggcggcgag aagtagggtc
     2941 agggtggcgg tgatgtttga gaccgcttcc actatttccg cctggttcat cacctggaaa
     3001 tcggcttcat ccgggttggc gatgtcgtgc tcgtcgagaa ggatggcttt ggtctgttct
     3061 tcgacaaaag acatgaccgc ggtcgattcg gcggcgatgt cgatcgtcga aagccggttg
     3121 cgtccgacaa gcgatctttg gatcgtagtc aaaggaatga aaatcgtatc gtcttgattc
     3181 atcaacccgc ccttgctttt cgagacgccg ataactttga atttcaagcc gttgatttta
     3241 acggtctcgc cgatcggatc ggctccttcg ccgaaaaggt cttcgacaac cgtcggtccg
     3301 agcacggcca ccctcgaagc gtttcttagg ttctgttcgg aaatgaaatc tccctgtgcc
     3361 acttcgagat tcttgacgat cgcgtattcc ggctcggttc cgataaccga ggtgttggtg
     3421 ttgttgccgt tcgcgatcac ctggtatctt ccggaaagct ctccggccac gcctttcact
     3481 ccgacaagct cggctttaag gcgtccgaga tcgtcgttgt cgagcgattg ggccg
//
LOCUS       DAOPQT010000050         3515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_28965_length_3515_cov_3.042486, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000050 DAOPQT010000000
VERSION     DAOPQT010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28965_length_3515_cov_3.042486"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PKG74_01765"
     CDS             complement(<1..438)
                     /locus_tag="PKG74_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOB90038.1"
                     /translation="MTNEEIARVFGEIGEYLEMGDVAFKPQAYSRASAVIASLEEDVA
                     SIYEKGGLKALEDIPGVGKSLALKIEEYLRTGKIKYYESLKKKTPVDVSELTRIEGLG
                     PRKIKILYKKLGIRNVDDLEKAASAGKISKLPGFGKKSEENILK"
     gene            complement(435..1088)
                     /locus_tag="PKG74_01770"
     CDS             complement(435..1088)
                     /locus_tag="PKG74_01770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOB90039.1"
                     /translation="MNIFKRFRINRVIRSFIISDLVLFSGWGMISPLFSIFVTGQVQG
                     ATLETVGVTTAIYWLVRSIAQMPLATALDRIKGEKDDFYTLIAGLVLVSISSFLYVFV
                     KTVPQLYTVHAFQALAFAFYAPSWSGIFSRHIDKDKAAVTWSIEHAFLGAASGISGYF
                     GSVIVANFGYQAVFIAASILSLIAAVVIFLIPDIVFPRPKRKQALPEMRNHSVKTIQ"
     gene            complement(1093..3420)
                     /locus_tag="PKG74_01775"
     CDS             complement(1093..3420)
                     /locus_tag="PKG74_01775"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOB90040.1"
                     /translation="MSSFLRQDSARRELTRLPQKGRLNAADYLSPSSLDALSAALREQ
                     QVLSGDYDLWLAKALFEFPEVIWAFERLDVEPTEVIQKINDYLAREPKPKQTREEVDK
                     RIEEIVLRAFEKSIDEVSNYVMPVDVMWALATISENCSKICALFDIDAEDLDEALIFG
                     RVKKVFKRKTGGVSITIGGLAGQMSGVRHRTVNRAWTSRPTPTLDMFGTDFTDIARAG
                     QTGFLIGHERVYQRMVNVLGRGNRPNALLLGLPGVGKETIVGHLAYMITRDKVPLALF
                     DKRLVSLDVSSLVSNADQPELQRRINQIFHEIKMAGNIILYIPNIHNLSRTSGERQLS
                     AANIILPLIIANDFPTIGATSPQEYREYIEKDSAFMDAFEVIRVEEISEDEAIRFLIY
                     DMLMSEKNNRTMISLGAIKTAVSLSRKYFREKPLPSSASDLLHEAWAWALENKVKRLT
                     SEMIVKIAEQKTNIPVRKAGKEEAEQLLNIENLIHQRLVDQNEAVSAVGRSLREYRSG
                     LSRKGGPIAAFLFVGPTGVGKTELAKILAEIQFGSEDAMVRVDMSEYQDESSYGRLIG
                     ASGGNIGGMLTDPVRQKPYSLVLLDEFEKAHPDVLNLFLQIFDDGRLTDAMGRVVDFE
                     NTIIIATSNAHSTFIKSSIDEGKTMAMIGDELKRKLVDYFKPELLNRFSEIVVFKSLS
                     EKDVESIAGLQLNSLAKSLDKTQGISMRFEEAATKEIARLGYDPAFGARPLRETISKH
                     IKSPIAEKILRDEIRRGSEVIIGFTDDGFKFDIIK"
BASE COUNT          806 a          907 c          929 g          873 t
ORIGIN      
        1 cttcagaata ttttcttcgc tttttttccc gaatccaggg agtttgctta ttttcccggc
       61 cgaggccgct ttttcgagat cgtcgacgtt ccttattccg agttttttgt aaaggatttt
      121 tattttccgc ggacccaagc cttcgatcct cgtaagctcc gacacgtcga ccggcgtctt
      181 cttcttgaga ctctcgtaat attttatttt tccggttctg agatactctt cgattttcaa
      241 agccagggat tttcccactc cgggaatgtc ttccaaagct ttcaagccgc ctttttcata
      301 gatcgaagcg acgtcttctt cgaggctggc gatgaccgcc gacgcccggc tgtaagcctg
      361 aggcttgaaa gccacatcgc ccatttcgag atactcgccg atctccccga aaacgcgggc
      421 tatttcttcg ttcgtcattg gatcgttttc accgaatgat tcctcatctc cggcaaggcc
      481 tgttttcttt taggacgggg aaagacaatg tccggaataa ggaaaatgac gacggccgct
      541 atcagggaaa gaatcgaggc ggcgatgaaa accgcctggt atccgaaatt ggcaacaatg
      601 acgctcccga aataaccgga gattcccgaa gcggctccca aaaaagcgtg ctcgatggac
      661 caggtgacgg ccgccttgtc tttgtcgatg tgtctggaaa atatgccgga ccaggagggc
      721 gcgtaaaaag caaaggccag cgcctggaaa gcgtgaaccg tgtaaagctg gggaacggtt
      781 ttcacgaaaa catacaggaa agaagaaatg gaaacaagca ccagtccggc gatcaaggtg
      841 taaaaatcgt ctttctcgcc cttgattctg tcgagagccg tcgcgagcgg catctgggcg
      901 atcgatctaa ccaaccaata aatggcggtg gtcactccga ccgtttccag ggtcgcgcct
      961 tgaacttgac cggtgacgaa aatggaaaaa agaggagaaa tcatacccca gccgctgaaa
     1021 agcaccaagt ccgagatgat gaacgacctg atgacgcgat tgatgcggaa tcttttgaaa
     1081 atattcataa aattatttaa ttatatcaaa cttgaatccg tcgtccgtga atccgattat
     1141 gacttccgat ccccttctga tctcgtcccg gagaatcttt tcggcgatgg gcgatttgat
     1201 atgtttggag atcgtctccc gaagcggtct cgcgccgaaa gccggatcat atcccagtct
     1261 cgcgatttcc ttcgtcgccg cttcttcgaa acgcatggat atgccctggg ttttgtccaa
     1321 ggatttcgcc aaggaattca gctggagccc ggcgatcgat tccacgtctt tttcggaaag
     1381 cgatttgaaa acaacgatct cggaaaagcg attcaaaagc tcgggcttga aataatcgac
     1441 cagttttctt ttgagctcgt cgccgatcat ggccatggtt ttgccttcat cgatggagct
     1501 cttgatgaaa gtggaatggg cgttcgaggt ggcgatgatg atggtgttct cgaaatcgac
     1561 cacccggccc atggcgtcgg tgaggcgacc gtcgtcgaat atctgaagaa aaagattcaa
     1621 aacatccgga tgggctttct cgaactcgtc gagcagaacc aagctgtagg gtttttgtct
     1681 gactggatcg gtaagcattc cgccgatgtt tccgccgctc gcgccgatca atcggccgta
     1741 actcgattcg tcctggtatt cggacatatc gacgcggacc atggcgtctt cggaaccgaa
     1801 ttggatctcg gccaggatct tcgcgagttc ggttttgccg acgccggtcg gaccgacaaa
     1861 aagaaaagcg gcgatcgggc ctccttttct agaaagcccg cttcgatatt cccgaaggga
     1921 gcggccgacc gcggaaacgg cttcgttttg gtcgacgagc ctttgatgga tcagattctc
     1981 gatgttcaaa agctgttcgg cttcttcttt cccggctttc cgcaccggaa tgttggtttt
     2041 ttgctcggcg atcttgacga tcatttccga ggtcaatctt ttcactttgt tttccaaagc
     2101 ccaggcccag gcttcgtgga gcagatcgct ggccgaagaa ggcaagggtt tttccctgaa
     2161 atatttgcgg ctcaaagaaa cggctgtttt gatggctccg aggctgatca tcgtgcggtt
     2221 gttcttctcc gacatcagca tgtcgtagat caaaaatctg atggcttcgt cttcggatat
     2281 ttcctcgacc ctgatcacct cgaaagcgtc catgaaagcg ctgtctttct cgatatactc
     2341 gcggtattcc tgggggctgg tggcgccgat tgtcgggaaa tcgttggcga tgatcaaagg
     2401 caggataata ttcgccgccg aaagctggcg ttcgcccgaa gtgcggctca aattgtggat
     2461 attcgggatg tacaaaatga tgtttccggc catcttgatc tcgtggaaga tctggttgat
     2521 ccgccgctga agctccggct ggtcggcgtt ggaaacaagg ctcgaaacat ccaaagaaac
     2581 gagcctttta tcgaacaaag cgagcggcac tttgtcgcgg gtgatcatat aggccagatg
     2641 cccgacgatc gtttccttgc cgactccggg aagtcccaga agaagcgcgt tcggcctatt
     2701 gccgcgaccc aagacattca ccatccgctg gtaaacccgc tcgtgtccga tgaggaatcc
     2761 ggtttgaccg gctcgggcga tatccgtgaa atccgttccg aacatgtcta aggtcggcgt
     2821 cggtctcgaa gtccaggccc gattcaccgt gcggtgtctc actccggaca tctgtccggc
     2881 cagaccgccg atcgtgatgg aaacaccgcc ggtttttctc ttgaaaacct ttttgacgcg
     2941 gccgaagatc aaagcctcgt ccaaatcttc ggcatcgata tcgaacaagg cacagatttt
     3001 cgagcaattc tcggatatgg tggcgagggc ccacatcacg tcgaccggca tcacgtaatt
     3061 gctcacctcg tcgatcgatt tctcgaaggc ccggagcacg atttcctcga tccttttgtc
     3121 gacttcttcg cgagtttgtt tcggtttcgg ctcgcgggcc agataatcgt ttatcttctg
     3181 gatcacctcg gtcggttcga catcgagtct ctcgaaagcc cagataacct ccgggaattc
     3241 gaaaagtgct ttggcgagcc agagatcata gtccccggac aagacctgct gttctctcaa
     3301 ggcggcggac aaagcgtcaa ggctcgaagg cgaaagatag tcggcggcgt tcagccgtcc
     3361 tttttggggg agtctcgtaa gctcgcgcct ggccgagtcc tgacggagaa aagaactcat
     3421 aagataaagg ctgaggatga tgcccggtca gaaaagttcc ggcaccttgg aaaggatgaa
     3481 aatgaaagcg gcggcggaga aaaccaaggt gatcg
//
LOCUS       DAOPQT010000051         3488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_29272_length_3488_cov_3.563064, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000051 DAOPQT010000000
VERSION     DAOPQT010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29272_length_3488_cov_3.563064"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PKG74_01780"
     CDS             <1..433
                     /locus_tag="PKG74_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HOB90041.1"
                     /translation="SLMAVLGLNVDILVPAALENAINEENVSKINAKQILEMANGPVT
                     PKAEEILTNKKVLIVPDVLANAGGVAVSYFEWVQNLQNYYWEKEEICAKLKKLMDSAF
                     TSVWAEYERMSKTTKKATLRQAAYSLAVQKVVKAMQARGEL"
     gene            433..1389
                     /locus_tag="PKG74_01785"
     CDS             433..1389
                     /locus_tag="PKG74_01785"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOB90042.1"
                     /translation="MSPKKRIAVLMGGPSSEHEVSLSSGKTVAKLLDRKKYEVFPVLI
                     DKKGKWDEDPRQLKDLIDLAFIAMHGEYGEDGAIQSVLEEAGLPYTGSGPASSALGMN
                     KVMSERVFRTAGLNPPESLLLQHFGDLGNLRIDFGWPLVVKPADKGSSVGVSIVQSPE
                     ELYPALEQAFAFSHNVMIQEFVKGRELTCGVIDDGLGNARALPPTEIVPKSAGFFDYE
                     SKYIPGASEEITPPDLPIDFIQLIQKTALAAHQAIKASGLSRTDMILSPYGKLYILEI
                     NTIPGMTPTSLLPQEALACGMSFPEFLDHVVQAAFRKYGLSD"
     gene            1373..3139
                     /gene="dnaG"
                     /locus_tag="PKG74_01790"
     CDS             1373..3139
                     /gene="dnaG"
                     /locus_tag="PKG74_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOB90043.1"
                     /translation="MASPIEEIKSKLDLVDFLRGYLDLKPAGKNLKANCPFHKERTPS
                     FMVSPDRQIWHCFGCNEGGDIFKFLMKYENLEFYEALQVLAEKAGVELRRISPADQRE
                     FGVLYDIARISAEFYRKNLEASEIARKYLEKRGLTVESIENFEIGFAGQSFEDLTLHL
                     INAGFDVKDLTRSGMTIKSEKGKYFDRFRGRIMFPLWNAFGKPVGFSGRILPEYETEE
                     TAKYLNSPETPIFSKSKIIYGLIQAKEAIREAGFVLVVEGMMDVIASHQDGVKNTIGI
                     SGTALTLDQLKLLHRHTNKLVFNLDNDPAGKQATERSIDLALENDFEARVLDLGQLAA
                     EKDLKIKDTADLVLLKSGILKELVGQSVSGMECYCREFLKDGDIGDRKQGIRMALQKV
                     RKIASAIEKDYWLKQISDRTGVREASLREEMEKPGMASGQSLKKVDTTTDLRDIKLSR
                     RELISERLLGCFSGDDDSKNSVEKVKEFLPQKYLEVYYCLSEGRLPESEELTGLANLI
                     NLRSGLADDSAAEVSRAVEMEELCRNLEMEFWTEKRDHLKSLIQTAEEKEDAKIVEYI
                     ADFQNITARIEFLKNQKFLNNG"
     gene            3132..>3488
                     /locus_tag="PKG74_01795"
     CDS             3132..>3488
                     /locus_tag="PKG74_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HOB90044.1"
                     /translation="MAKNKKKRSARKPVRKTKKPAKASRKIVRPKKSRKPAKPKIAKK
                     VSKKTAKPSVRKKEKPRKFKFDLGVLDELINQGKAKGFVTDLEILKFFPRIETDVPFL
                     EHIYDRIEEAGLRVIDV"
BASE COUNT          962 a          823 c          948 g          755 t
ORIGIN      
        1 ttcgttaatg gcggttttgg gcttgaacgt cgatattctc gtgccggccg ctttggaaaa
       61 cgccattaac gaagaaaacg tttccaagat caacgccaag cagattctcg agatggccaa
      121 cggcccggtg actccgaaag ccgaagagat cctgaccaac aagaaggtct tgatcgttcc
      181 cgacgtattg gcgaacgccg gcggcgtggc ggtttcttat ttcgaatggg tccagaatct
      241 ccaaaattat tattgggaaa aagaagagat ctgcgcgaag cttaaaaaac tgatggactc
      301 cgcttttact tcggtctggg ccgagtacga acggatgtcg aaaacgacga agaaggcgac
      361 tctgaggcag gcggcttatt ctttggccgt tcaaaaggtc gtcaaagcca tgcaagccag
      421 gggagaattg tgatgtctcc gaaaaaacga atcgcggttt tgatgggagg accttcgtcc
      481 gaacacgaag tgtctttgtc gagcggcaag accgtggcca aacttttgga caggaaaaaa
      541 tacgaagttt ttccggtttt gatcgacaaa aaaggaaaat gggatgagga tccgcgccag
      601 ctgaaagatc tgatcgatct cgctttcatc gccatgcacg gcgaatacgg cgaggacggc
      661 gcgatccagt ctgttctcga ggaagcgggc ttgccttata ccggaagcgg tccggcttcg
      721 agcgccctcg gtatgaacaa agtgatgtcg gaaagggttt tccgaaccgc cggactgaat
      781 cccccggaat cgcttttact ccagcatttc ggagatttag gaaatctgcg catagatttc
      841 ggctggccgc tcgtggtcaa accggcagat aaaggttcgt ccgtcggcgt gtcgatcgtc
      901 cagtctccgg aagaattata tccggccctg gaacaggctt tcgctttttc ccataacgtg
      961 atgatccagg aattcgtcaa aggccgagaa ctcacctgcg gtgtcatcga cgacggactc
     1021 ggaaacgcga gggctttacc cccgacggag atcgtcccga aatcggccgg gtttttcgac
     1081 tacgaatcca aatatattcc cggcgcttcc gaagaaataa cgcctccgga tttgccgata
     1141 gactttatcc agcttatcca aaaaacggct ctcgccgccc atcaggcgat caaagcctcg
     1201 ggattgtcga ggacggacat gattttgtcc ccctacggca aattgtatat attggagata
     1261 aacaccatcc cgggaatgac gccgacgagc cttttgccgc aagaagcttt ggcttgcgga
     1321 atgagttttc cggagttctt ggatcacgtc gtccaagcgg ctttccgcaa atatggcctc
     1381 tccgattgaa gaaataaaat ctaagctcga cctggtcgat tttctccggg gttatcttga
     1441 tttgaagccg gccggaaaaa atctgaaggc gaattgtccg tttcacaagg aacggacgcc
     1501 atctttcatg gtttcgccgg acagacagat ttggcattgc ttcggctgca acgaaggcgg
     1561 cgatattttc aagttcttga tgaaatacga gaatctggag ttttacgaag cgctccaggt
     1621 tctcgcggaa aaagccgggg tggaactccg gcgcatcagt ccggcggatc agcgcgaatt
     1681 cggcgtgctt tacgatatcg cgaggatatc cgcggaattt taccggaaaa atctcgaggc
     1741 gtcggaaatc gcccggaagt atctggagaa aaggggattg accgtcgaaa gcatcgaaaa
     1801 cttcgagatc ggtttcgccg ggcagagttt cgaagacttg acgctccatc tgatcaatgc
     1861 cggcttcgac gttaaagatt tgacgcgttc cggaatgacc attaaaagcg aaaaagggaa
     1921 atatttcgac cgtttccgcg gacggatcat gttccctctt tggaacgctt tcggaaaacc
     1981 ggtcggtttt tccggccgga ttttgccgga atacgaaacc gaggaaacgg ccaagtattt
     2041 gaattctccg gaaactccga ttttctccaa atcgaaaatc atctatggac tgattcaggc
     2101 gaaagaggcg atccgcgaag cgggattcgt ccttgttgtc gaaggaatga tggatgtgat
     2161 cgcgagccat caggacggag tgaaaaacac gatcggcatt tcggggaccg ctctgacgct
     2221 cgaccagttg aaattgcttc atcggcacac caacaaattg gttttcaatc tcgataacga
     2281 tccggccggc aagcaggcga ccgaacggag catcgatctc gccctcgaaa acgatttcga
     2341 ggcgcgggtt ctcgaccttg gccagctggc cgcagaaaaa gacctgaaaa tcaaggacac
     2401 ggccgatctg gttttgttga aatcggggat tttgaaggaa ctcgtcggtc agagcgtgag
     2461 cggaatggaa tgttattgcc gggagtttct gaaagacggc gatatcggcg acagaaaaca
     2521 gggcatcaga atggcgctcc agaaagtgag aaaaatagcg agcgctatcg aaaaagacta
     2581 ttggctgaaa cagataagcg accggaccgg cgtcagggaa gcgagcctgc gggaagagat
     2641 ggaaaagccg ggaatggcct ctggccagtc gctcaaaaaa gttgacacga caacggattt
     2701 acgggatatt aagctatcaa gacgggagtt gatcagcgaa aggctgcttg gctgtttttc
     2761 cggagacgac gactcgaaaa attccgtgga aaaagtcaaa gaattcctcc cgcaaaagta
     2821 tctcgaagtg tattattgtc tttccgaagg aaggcttccg gaaagcgagg aattgaccgg
     2881 cctcgccaat ctgatcaatc tacgatccgg cctcgctgac gattcggcgg ccgaagtctc
     2941 cagagccgtg gaaatggaag aactttgccg gaatttggaa atggagttct ggacggaaaa
     3001 gcgcgaccat ttgaaatcgt tgatccagac ggcggaagag aaggaggacg ctaaaatcgt
     3061 cgaatacatc gcggatttcc aaaacatcac cgcgagaata gaatttttaa aaaaccagaa
     3121 atttttgaac aatggctaaa aacaagaaga aaagatccgc gagaaaaccg gtgcgtaaaa
     3181 caaagaagcc ggccaaggct tcgcggaaaa tagttaggcc gaagaaatcc agaaaaccgg
     3241 ccaagccgaa gatcgccaaa aaagtttcca aaaagaccgc caaaccgtcg gttcggaaaa
     3301 aagaaaaacc ccgcaaattc aaatttgatt tgggagtctt ggacgagctt atcaaccagg
     3361 gtaaggccaa gggatttgtc accgatctcg agattctgaa attctttccg cggatcgaaa
     3421 ccgacgttcc tttcctcgag catatctacg accggatcga agaagcggga ctccgcgtca
     3481 tcgacgtc
//
LOCUS       DAOPQT010000052         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_30532_length_3377_cov_3.061108, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000052 DAOPQT010000000
VERSION     DAOPQT010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30532_length_3377_cov_3.061108"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PKG74_01800"
     CDS             complement(<1..453)
                     /locus_tag="PKG74_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90045.1"
                     /translation="MAERLKSVWKTPKLRLGVKAACLAASFFYLPFWASALLASLFYF
                     YPALNTEKLLASFLSSLFLGFVFSGIMSAADLWLVSAVIFYLLLGVKDMVFLNQKSVY
                     GLLFSVLMIAGFFGVLSGAISPWILALLIFILFREFFSIILPSYPKRAR"
     gene            complement(398..3016)
                     /gene="clpB"
                     /locus_tag="PKG74_01805"
     CDS             complement(398..3016)
                     /gene="clpB"
                     /locus_tag="PKG74_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOB90046.1"
                     /translation="MNSFNRFTLKAQEALQSAQDIASSMKHGEFRSLHLLYALIGDSE
                     SLVQPILEKLNVDFTALEDDINNELRRLPKIFGEANVSQMYLSQEVVKVIEAGVRFSQ
                     VNKDEFISCEHLLLGVFEVVSPAKILLERHGVTREGLMRALAELRGSAKVTDETPESK
                     FQVLEKYSENLTSKAREGKLDPLIGRDEELRRVIQVLSRRTKNNPVLIGEPGVGKTAI
                     VEGLAQKIVSGDVPETLKGKEIVSLDLGSLIAGTKFRGEFEDRLKAFIKEIKNSSGKL
                     ILFIDEIHMIIGAGSAEGAMDASNLLKPSLARGELHAIGATTTKEYQRHIEKDAALER
                     RFQPIMVEEPSLEDSVTILRGLKEKYEVHHGLKISDEALVAAVNLSARYISDRFLPDK
                     AVDVIDEASAARKLESESVPQSIDSLKKQITSLEIEKKALKKETGSESRQKTIDKSLK
                     DLKKQYDDLSGEWQAERMVFENVHNLRKRIEDLRRSAEVAERDGDLETVAKIRYGELP
                     QAEKEFNAYEKKHFGAKARKERFIKEQVDAEDVAKVISRWTGVPVANMLESEAEKLTR
                     IEEELEKRVIGQEEAVSAVSNALRRSRAGLADPNRPLGSFMFMGPTGVGKTELARALA
                     DFMFNRDDAIVRIDMSEYMEKHSVARLIGSPPGYVGYEEGGQLTETVRHKPYSLILFD
                     EIEKAHPEVFNVLLQVLDNGRLTDGKGKTVNFKNTIIILTSNVGSELNREISRVGFHM
                     SADEFKSKEEDYRTRMMEALRDRFRPEFLNRLDEIIVFHSLTKTDILKIVDIQIEGLR
                     KQLAERGIKATIDQETRKYIAEKGYDPEFGARPLKRFIQKTILDRLADQLIRKEIKNG
                     GKIKISLENSEIKIRS"
     gene            complement(3197..>3377)
                     /locus_tag="PKG74_01810"
     CDS             complement(3197..>3377)
                     /locus_tag="PKG74_01810"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HOB90047.1"
                     /translation="IQTDTAINPGNSGGPLLNLKAEVIGINTAIVSGAENIGFAIPAN
                     KVKTVMVSLLEGKAE"
BASE COUNT          801 a          879 c          814 g          883 t
ORIGIN      
        1 cctggcccgc ttcggatagc tcggaagaat aatcgagaaa aactcccgga aaagaataaa
       61 aatcaacaat gccaaaatcc agggggaaat cgctccggac aaaaccccga aaaacccggc
      121 gatcatgaga acggagaaaa gcaatccgta aaccgatttc tggttcaaaa aaaccatgtc
      181 tttcactccg agaagcaaat agaaaataac ggcggagacg agccaaagat ccgccgccga
      241 catgattccg gaaaaaacaa atcccaaaaa caaactcgat aaaaaagaag ccaggagctt
      301 ttccgtattc agagccgggt aaaaataaaa cagcgaggcg agcaaagccg aagcccaaaa
      361 aggaagatag aagaacgaag ccgccaaaca ggcggcttta actcctaatc ttaatttcgg
      421 agttttccag actgatttta atctttccgc cattttttat ttctttcctg ataagttggt
      481 cagccagccg gtcgaggatg gttttctgga taaagcgctt caaaggcctg gcgccgaatt
      541 ccggatcata gcctttttcg gcgatgtatt ttctggtttc ctggtcgatc gtcgccttga
      601 ttcccctttc ggcaagctgt tttctcaatc cttcgatctg gatatcgacg atcttcagaa
      661 tgtccgtttt ggtcaaagaa tggaaaacaa taatctcgtc caaacggttc aaaaactccg
      721 gtcggaagcg atcgcgcaag gcttccatca ttctcgtccg ataatcttct tccttgctct
      781 tgaactcgtc ggccgacatg tggaatccga cccgcgagat ctcgcggttc aattccgagc
      841 cgacgttcga agtcagaata atgatcgtgt tcttgaaatt gacggttttg cctttgccgt
      901 cggtaagtct tccgttgtcc aaaacctgca aaagtacgtt gaaaacttcg gggtgggctt
      961 tttctatttc gtcgaaaaga atcaggctgt aaggtttgtg tcgcacggtt tcggtaagtt
     1021 gtcccccttc ttcgtagccg acatatcccg gaggcgaacc gatcaaacgg gccaccgaat
     1081 gcttttccat gtattcggac atgtcgatcc tcacaatggc gtcgtcgcga ttgaacataa
     1141 agtcagcgag agcccgggcg agctcggttt taccgacgcc ggtcggaccc atgaacatga
     1201 acgatcccag gggtctattc gggtcggcca acccggcgcg actccgacgc aaagcattcg
     1261 aaaccgcgga cacggcttct tcctggccaa tgacccgttt ttccaattct tcttcgatcc
     1321 gggtcagttt ctcggcttcg gactcgagca tgttcgccac gggcacgccg gtccagcggg
     1381 aaatgacttt ggccacgtcc tcggcatcga cttgctcctt gataaaccgt tcttttcgcg
     1441 ctttggcgcc gaaatgcttt ttctcgtagg cgttgaattc tttttccgcc tggggcaatt
     1501 cgccgtagcg gatcttggcc acggtttcca gatctccgtc gcgttcggcc acttcagccg
     1561 agcgccgcag atcctcgatc ctttttctca aattgtggac gttctcgaaa accatccgtt
     1621 cggcctgcca ttctccggaa aggtcgtcgt attgcttttt caggtctttc agggatttgt
     1681 cgatagtttt ttgacgggat tcgcttccgg tttctttttt caaagccttt ttttctattt
     1741 ccagactggt gatctgtttt ttcaaagagt cgatcgattg gggcacggat tcggactcga
     1801 gcttcctcgc ggccgaagcc tcgtcaatga catccacggc cttgtccggc aaaaacctat
     1861 cggaaatata gcgggcggaa agattgacgg ccgcgaccaa agcttcgtcc gagattttca
     1921 agccgtgatg aacttcgtat ttttccttga gccccctcaa aatggtgacg ctgtcttcca
     1981 aagacggttc ttcgaccatg atcggttgga aacggcgttc caaagccgcg tctttttcta
     2041 tgtgccgctg atattctttg gtggtcgtgg cgccgatcgc gtgcaattcg ccgcgggcca
     2101 agctcggttt caacaaattg gaggcgtcca tggcgccttc agccgaaccg gctccgatga
     2161 tcatgtggat ctcgtcgata aaaagaatca atttaccgct cgaattcttg atttccttga
     2221 taaaagcctt taatcggtct tcgaattctc cgcggaactt ggtgccggcg atcaaagagc
     2281 ccaaatcgag tgaaacgatc tcttttcctt tcaaggtttc cggaacgtct ccggaaacta
     2341 ttttctgggc caaaccctcg acaatggcag ttttgcccac acccggctcg ccgatcaaaa
     2401 ccggattgtt tttggtgcgg cgggacaaaa cctggatcac tcggcgcaac tcttcgtcgc
     2461 ggccgatcaa aggatcgagt ttgccttcgc gggccttgct cgtcaggttt tccgaatatt
     2521 tttccaaaac ctgaaatttg gattccggag tttcgtcggt cactttagcg ctccctctaa
     2581 gctcggccaa ggcccgcatc agtccttcgc gggtgacgcc atggcgttcg agaagtattt
     2641 tggccggaga aacgacctcg aaaaccccca aaagaaggtg ttcgcaggaa ataaactcgt
     2701 ctttgttcac ctggctgaag cggactccgg cttcgatcac cttgaccact tcctgggaaa
     2761 gatacatctg ggaaacgttc gcctctccga aaatcttcgg caaacggcgc aattcgttgt
     2821 tgatgtcgtc ttcgagagcc gtgaaatcca cgttcagttt ttccaaaatc ggctggacca
     2881 aggattcgga atcgccgatc agggcgtaca aaagatgaag actgcggaat tctccgtgct
     2941 tcatggacga agcaatatcc tgcgccgact ggagagcttc ctgggctttt aaagtgaaac
     3001 gattgaaaga attcatgatg atttacgcta attatgacac aactcggttg aaataaaata
     3061 acaatttcgg attccggatg tcaaataatc cgtgcttcca aatctcgacc agatgccttt
     3121 cgagcggttt ttccacaatt ttgtgtgtta acgcttgtta agccattgat caatggctta
     3181 acagccgcta aggaacttat tctgcttttc cttccaaaag actcaccatg accgtcttta
     3241 ccttgttggc tggaatggca aagccgatgt tctcggcgcc ggacacgatg gccgtattga
     3301 tgccgatgac ctcggccttc aggtttaaga gagggccacc ggagttgccg gggttaatgg
     3361 ccgtgtccgt ctgtatg
//
LOCUS       DAOPQT010000053         3372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_30584_length_3372_cov_3.634911, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000053 DAOPQT010000000
VERSION     DAOPQT010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30584_length_3372_cov_3.634911"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PKG74_01815"
     CDS             <1..292
                     /locus_tag="PKG74_01815"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOB90048.1"
                     /translation="KKEIERDIENFRLHEASNKLYHYFWHVFADKIIEESKPRLKENK
                     DAADKAAAGETLLRMLRELIKMLHPFMPFTTEEVYQSLPDKKQTCLMAESWN"
     gene            283..942
                     /locus_tag="PKG74_01820"
     CDS             283..942
                     /locus_tag="PKG74_01820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOB90049.1"
                     /translation="MELTFLKFLISGGIGLALGIVWLSFFLLEDDEHPEPKSLLFATF
                     VLGLASAWVAIACEQIFAVHSPAWGIANYSRLSLVVNSFIEESIKFGVIWIFIRKSRF
                     FDEPIDRMIYMITASLGFATMENLLFFLSVAMNMNELVNLALLRFIGATLMHAIASGF
                     LGYFWSRKKIVWGVAGAILIHAGFNEMILKSGPALMPTIFLIVLSFLLFGYFDKLRET
                     I"
     gene            939..1319
                     /locus_tag="PKG74_01825"
     CDS             939..1319
                     /locus_tag="PKG74_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOB90050.1"
                     /translation="MNEENNFDLAKIAEESEGQLTIDVYQTPDEIFVESTIAGVEPED
                     LDIDITNESVTIKGERRKEEVIADEDYLHQECYWGRFSRSVILPQEIDPDKARADFKN
                     GILKIRLPKLNRGRVKKLKIKQEE"
     gene            complement(1423..1785)
                     /locus_tag="PKG74_01830"
     CDS             complement(1423..1785)
                     /locus_tag="PKG74_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90051.1"
                     /translation="MDFFEELRQRAMNEGVSPVLMKAVLNRLENSDVGKRLQFIVSIN
                     SHGPPLVAYAVEFVRFREEIIRGKNGNSDRVSAAWKRLIEKGFSQDLLMTLNKEILKA
                     TPSPVSISAARTPRRFPH"
     gene            2083..2325
                     /locus_tag="PKG74_01835"
     CDS             2083..2325
                     /locus_tag="PKG74_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOB90052.1"
                     /translation="MPRFDGTGPMGQGARTGRGLGPCGGGMRRGWGWCGGYGFGFRKF
                     ISPKNELAALEDEEKMLEEELAAIKEEKAALKNQQK"
     gene            2409..2687
                     /locus_tag="PKG74_01840"
     CDS             2409..2687
                     /locus_tag="PKG74_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOB90053.1"
                     /translation="MPRPRLCRKIEFDPNITYFKPQGVPLRYLEIVELTTEEMEAYRL
                     RHINNLEQKQAADKMRTSQSTYQRILYSAYKKIADALINGKAIKIIKQ"
     gene            2698..>3372
                     /locus_tag="PKG74_01845"
     CDS             2698..>3372
                     /locus_tag="PKG74_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOB90054.1"
                     /translation="MKKHLLILGLVILAIASISAIAVKNRPINTMLMLIEFEKVDGIL
                     QWEKELDKRDLTALVKVQDNVLEKNPETFKRLADKGYEVAGGYDEAPFWDMSYDEQYK
                     RLKESKEKIERITGEKMRVFGSRYFAYDETTLKAADALGIEYILGRGVNDVEATVYQP
                     KEYKTKIISVTNVDVGEPMGRGSLCDYSLWARGADSQEFGKILDESIAKKPNNMIIVS
                     HAYLGGT"
BASE COUNT         1018 a          751 c          789 g          814 t
ORIGIN      
        1 caaaaaagaa atcgagcgcg acatcgagaa tttccggctc cacgaagcgt cgaacaagct
       61 atatcactat ttctggcacg ttttcgcgga caagataatc gaggaatcca aaccgagact
      121 caaggaaaac aaagacgcgg cggataaagc cgcggccggc gaaacccttc ttcgcatgct
      181 tcgggagctt atcaagatgc ttcacccctt catgcctttc acgaccgaag aagtctatca
      241 atcccttccg gataaaaaac aaacctgttt gatggcggaa tcatggaact gacttttctc
      301 aagtttttga tttccggagg catcggcctt gctctcggca ttgtttggct ttccttcttc
      361 cttttggaag acgacgaaca tccggaaccg aaaagcctgc ttttcgccac ttttgttctc
      421 ggcctcgctt cggcctgggt ggccatagcc tgcgaacaga ttttcgccgt gcactctccg
      481 gcttggggaa tcgccaatta ttcccggctt tcgctcgtgg tgaacagctt catcgaggaa
      541 tcgatcaagt tcggcgtgat ctggatattc atccgcaaat cccggttttt cgacgaaccc
      601 atcgaccgga tgatctacat gatcaccgcg agtctcggat tcgccactat ggaaaatctg
      661 cttttcttcc tctcggttgc gatgaacatg aacgagctcg tcaatttagc cctgcttcgg
      721 ttcattgggg ccaccctgat gcacgccatc gcctcgggat tcttgggata tttctggtcg
      781 cgcaagaaaa tcgtctgggg cgtcgccggc gccattctga tacacgccgg attcaacgaa
      841 atgattttga aatccggccc ggccctcatg ccgacgatct tcttgattgt cctgtccttt
      901 ttattgttcg gttattttga taaacttaga gaaaccatat gaacgaagaa aacaattttg
      961 atctggcgaa gattgccgaa gaaagcgagg gacaattgac gatcgacgtt tatcagacgc
     1021 ccgacgagat tttcgtcgaa tcgaccatcg ccggcgtcga acccgaagat ctcgacatcg
     1081 atatcaccaa cgaatccgtc accatcaaag gcgaacgccg gaaagaagaa gtgatcgccg
     1141 acgaagacta tcttcaccag gaatgctatt gggggagatt ttcgaggtcg gtcatccttc
     1201 cccaggaaat cgatccggac aaagcgcggg cggatttcaa aaacggcatc ctgaaaatca
     1261 gactgccgaa gctgaatcgc ggccgggtaa aaaaactgaa aataaaacaa gaagagtaga
     1321 aatagtaagg ccccttcgtt gaacgaaagg gccttttaaa aggaactttg ttgcggacac
     1381 actggatctt ccataaatca aaccctccta gcaaattcga tgttagtgcg ggaaacgtcg
     1441 cggggtccga gcagcggaga tcgacaccgg agacggggtg gctttcaaga tctctttgtt
     1501 gagagtcatg agaagatctt gactaaagcc cttctcgatc agtcttttcc aagccgccga
     1561 aacccgatcg gaattgccgt ttttaccgcg aataatttct tcacggaaac ggacgaattc
     1621 caccgcgtag gcaaccagcg gggggccgtg actgttgatg gagacaataa actgcaaccg
     1681 ttttccgaca tcgctgtttt ccagccggtt cagtaccgcc ttcatcagaa cgggactgac
     1741 tccctcgttc atcgccctct gtcgcaattc ctcaaaaaaa tccatctcta accctccttc
     1801 ccattgatga tttcaatgtc cgaatccaac ccgatcggtt ggatcacttt ctctataaca
     1861 caaactcgtc gccagtcaac cggcggcgag tggacaaaaa aaatacagtt aagagccaaa
     1921 aatcaagtga ctttaagaag aataaattta gtacgtttat ccgaactttt ttaatcagat
     1981 ttttaaatta ttgacaatgg aatgttatgg gtgtatattc ataacagata gtaaataaag
     2041 gtcgagctat ttattaaaat taagaaagga aattaaaaaa atatgccaag atttgacgga
     2101 actggtccaa tgggacaagg cgcgagaacc ggccgtggcc tcggcccttg cggaggcgga
     2161 atgagacgag gctggggttg gtgtggcggc tatggattcg gcttcaggaa atttatttct
     2221 cccaaaaacg aactggccgc attagaagat gaagaaaaaa tgcttgaaga agaattagcg
     2281 gcgattaaag aagaaaaagc agcccttaaa aaccagcaaa aataaaagaa ggcgcgggaa
     2341 ccggcctctt tgcaaaaagg ggccgggtgg ccttctttca agaataattt atcataaaat
     2401 ataggaatat gccaagaccg agactttgca gaaaaataga atttgatccg aatataactt
     2461 atttcaaacc tcaaggcgta ccgctgagat atcttgaaat tgtggaactc actaccgaag
     2521 aaatggaagc atatcgtttg cgacatatta acaacttgga acaaaaacaa gcggctgaca
     2581 aaatgcgcac ttcgcaaagc acatatcaaa ggattctata ttcggcttat aaaaaaattg
     2641 ccgacgcttt gataaacggc aaagcgatta aaattatcaa gcaataatag aggaactatg
     2701 aaaaaacatc ttttgatttt gggattagtt attttggcga tagcttcaat atcggctatc
     2761 gctgtaaaaa atcggccgat caacaccatg ttaatgttga ttgagtttga aaaagtggat
     2821 ggaattttac aatgggaaaa agaactagat aagagagatc tgaccgcctt agtcaaggtt
     2881 caagataacg ttttggaaaa aaatccggaa acattcaaaa gattggccga taaaggatac
     2941 gaggtagccg gcggttacga tgaagctcct ttttgggaca tgtcgtatga cgagcaatat
     3001 aaacgcttaa aagaatcaaa agaaaagata gagaggatta ccggggaaaa aatgagagtt
     3061 tttggcagcc gctatttcgc ctacgacgaa accaccctga aggcggctga cgccttggga
     3121 attgaatata ttttaggccg cggagtcaac gacgttgaag caacggtgta tcagccaaaa
     3181 gaatataaga caaaaataat ttcggtaacc aatgtggatg ttggcgaacc gatgggacgg
     3241 ggttcgctct gtgattatag cctttgggcc agaggggccg attctcaaga attcggtaaa
     3301 atattggacg agagcatcgc caaaaaacca aataatatga tcatagtttc tcacgcttat
     3361 ttaggcggaa cc
//
LOCUS       DAOPQT010000054         3365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_30664_length_3365_cov_3.289426, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000054 DAOPQT010000000
VERSION     DAOPQT010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30664_length_3365_cov_3.289426"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PKG74_01850"
     CDS             complement(<1..537)
                     /locus_tag="PKG74_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOB90055.1"
                     /translation="MAVIGLAIFFAGFGQSILAKSEAKKLSQFIAANSALGSVLGLRQ
                     DGSLAASSEIVSQAEVISDGLNRERIMEGALFEASKTVEDDPETRTETIVYKVLSKDS
                     LASIAKKFNISIDTIAWANDLTNRTIKTNMNLFILPVSGVLHEIRSGESVESIADAYH
                     VPAQQIYAFNKGKILTEGV"
     gene            574..2319
                     /locus_tag="PKG74_01855"
     CDS             574..2319
                     /locus_tag="PKG74_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOB90056.1"
                     /translation="MSIIPQSLNDRQLEAATAPDGPLLITAGAGSGKTKTLTQRLAFL
                     LEKGVPAEQIVAITFTNKAAEEIKKRVYPEIKSRGLPFLGTFHSLGARILRQEPSFGR
                     TRGYVIFDDDDSSRLVKNIIKSLDLDKERFPGASFTGKISKIKSELLRPEDLGDDLAV
                     KIFEKYEKGLAEENAFDFDDLIEKTVRLFRQFPDVLGEYQRRFRHILVDEYQDINQSQ
                     YELVRLLASGHRNLSVVGDDCQSIYSFRGSDFRNFLNFEKDWPEAKIVTLDQNYRSTK
                     NIIAAAGGVIGHNTLQRPKNLWTDNPEGDLVHVFGAYGAEDEAAWLADKIIGLGDYEN
                     TAVLFRTNAQSRPIEQYFIYNQIPYQIYGGLKFYERKEIKDIVACLRYGFNPKDSISR
                     ERIETSFYRKPAIELVERLPEMAVALSPAELIGFILQTTDYPVFLATKFKNSEERMEN
                     IGELVSFAAESESLEELLEKVSLFQATDEMKGAERPGVKMMTIHLSKGLEFGNVFVCG
                     VNEGVLPHHRSLVSKDELEEERRLMYVAMTRAKEKLFLSFFNFPSRFLYEVPPELVEF
                     DGISPKTDEDTVYLD"
     gene            2319..3086
                     /gene="rsmA"
                     /locus_tag="PKG74_01860"
     CDS             2319..3086
                     /gene="rsmA"
                     /locus_tag="PKG74_01860"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOB90057.1"
                     /translation="MRRLGQHFLKNQEILRKIASALEISETDIIVEIGPGHGELTRHI
                     LDQNPKRLIAIEKDKNLAVKLADSLQKNNLEVILGDALKELPLLTRDLQSEYKLSGNI
                     PYYISGYLFRTMESLEKKPALSVFTVQKEVAGRIAAEPPQMNLLAASVCFWAEPKIIS
                     FVSKKDFSPAPKVDSAVILLKTRLSESPIDRKNYYPFIKALFKQPRKTILNNLSALET
                     NKEKLKEKLEKQGVDPSLRGQDLSFEEILELSRVFTG"
     gene            3106..>3365
                     /locus_tag="PKG74_01865"
     CDS             3106..>3365
                     /locus_tag="PKG74_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90058.1"
                     /translation="MKRKSLLLIFLAAILLTAFLGSPAIVSAQDAEQTESSSERTSGG
                     RVGTGEEQSGSLNCTLSLLSLNLTNILTCLLTNFVIEIVRAFV"
BASE COUNT          904 a          880 c          798 g          783 t
ORIGIN      
        1 cacgccctcg gtcaatattt tccctttgtt gaaagcgtat atctgctggg ccggcacatg
       61 gtaggcgtcg gcgattgact ccacggattc tccggagcgg atctcgtgga gcactccgga
      121 aaccggaaga ataaagagat tcatgttggt tttgatcgtc cgattggtta aatcattcgc
      181 ccaggcgata gtatcgatcg aaatattgaa ctttttggcg atggaagcga ggctgtcttt
      241 ggaaaggact ttgtaaacga tcgtctccgt tctcgtctcc ggatcgtctt cgaccgtttt
      301 cgaagcttcg aatagagctc cctccatgat ccgttcccgg ttcaggccgt cggaaatcac
      361 ctcggcctgg gacacgattt ccgaagaagc ggcgagcgag ccgtcctggc gcagaccaag
      421 aaccgatccc aaggccgagt ttgcggcgat gaactgcgat agctttttgg cttcgctttt
      481 tgctaaaata ctctggccga aacccgcaaa aaatatggcg agtcctataa ccgccaagag
      541 agcatatctc gatttgtggg tcagattact aatatgtcca ttataccaca aagcctgaac
      601 gatcgccagc tcgaggcggc caccgccccg gacggaccgc tcctcatcac cgccggcgcc
      661 ggatccggca agaccaaaac cctgacccaa agattggctt ttttgctaga aaaaggcgtt
      721 ccggccgaac aaatcgtcgc catcaccttc acgaacaaag cggccgagga aatcaaaaaa
      781 cgggtttatc cggaaataaa atcccgcggt ctgccttttt tgggaacatt ccattcgctc
      841 ggcgcgagaa tcctgcggca agagccgtct ttcggacgga ccaggggtta tgtcattttc
      901 gacgacgacg attcctcgag gctcgttaaa aacataatca agtctctcga tctcgacaaa
      961 gagaggtttc ccggagcgag tttcacggga aagatctcca aaatcaaaag cgagcttttg
     1021 cggccggaag acctcggaga cgatttggcc gtcaaaatct tcgaaaaata cgaaaagggg
     1081 ctcgccgaag aaaacgcttt tgatttcgac gaccttattg aaaaaaccgt ccggcttttc
     1141 cgccaatttc ccgacgttct cggagaatat cagcggcgtt tccgacacat cctggtcgat
     1201 gaataccagg acatcaacca aagccagtac gaactggtgc gattgctcgc ttccggacac
     1261 cgcaatttga gcgttgtcgg cgacgactgc caaagcatct acagcttccg gggatcggac
     1321 ttccggaact ttctcaattt cgagaaagac tggcccgaag ccaagattgt caccctggac
     1381 cagaattacc gctccacgaa aaacatcatc gccgcggccg gcggagtcat cggccacaac
     1441 acgctccagc ggccgaaaaa tctttggacc gacaatcccg aaggcgatct ggtgcatgtt
     1501 ttcggggcct acggagccga agacgaagcc gcctggctcg cggataaaat aatcggcttg
     1561 ggagactacg agaatacggc ggtgcttttc aggacgaacg cccaatcgcg accaatcgag
     1621 cagtatttca tctacaacca gatcccttac cagatctacg gcggattgaa attctacgaa
     1681 agaaaagaaa tcaaagacat cgtcgcctgc ctcagatacg gattcaatcc gaaagattcg
     1741 atcagccgcg aacgcatcga aacgtctttc tacagaaaac cggcgatcga gcttgtggaa
     1801 agacttccgg aaatggctgt tgcgctttct ccggccgaac tcatcggctt cattctccag
     1861 acaaccgatt atccggtttt tctcgctacc aaattcaaga attccgaaga acgcatggaa
     1921 aacatcggcg aattggtcag tttcgcggcc gaatcagaat ccctcgagga gctgctcgaa
     1981 aaagtctctc ttttccaggc gaccgacgaa atgaagggag ccgaacgacc gggagtcaaa
     2041 atgatgacca ttcacctttc caaaggactc gaattcggaa acgtttttgt ctgcggagtg
     2101 aacgaaggcg ttttgccgca ccaccgatcg ctcgtttcca aagacgagct cgaagaagaa
     2161 cgtcggctaa tgtacgtggc catgacccgg gccaaagaaa aattgttttt gagttttttc
     2221 aattttccct cgcgttttct ctacgaggtc ccgccggagc tcgtcgagtt cgacggcatc
     2281 agcccgaaaa cagacgagga tacggtatac ttggattgat gaggcgactc ggccagcatt
     2341 ttttgaaaaa ccaggaaatc ctccggaaga tcgcctcggc tttggagatt tccgaaaccg
     2401 acattatcgt cgaaatcggt ccgggccacg gagaactcac gagacatatt ttggaccaaa
     2461 atccaaaaag attgatcgcc atcgaaaaag acaagaacct ggcggttaaa ctcgctgatt
     2521 ctcttcaaaa gaacaatttg gaagtgatct tgggcgacgc cttgaaagaa ctgcctttac
     2581 ttacccgtga tttgcaaagc gaatacaaac tctccgggaa cattccttat tacatcagcg
     2641 gctatctttt ccggacaatg gaaagcctcg agaaaaaacc cgcgctttcc gttttcactg
     2701 tccagaaaga agtggccgga agaatcgcgg ccgagcctcc gcaaatgaat ctgctcgcgg
     2761 cgagtgtttg cttttgggcc gaaccgaaaa ttatttcttt cgtctccaaa aaagattttt
     2821 ctccggctcc gaaagtggat tcggcggtca ttcttctaaa aacccgattg tccgaatctc
     2881 caattgaccg gaaaaactac tatccattta taaaggcgct tttcaagcag ccgagaaaaa
     2941 ccatcttgaa caatctttcc gctctcgaaa caaacaaaga aaaactcaag gaaaagctcg
     3001 aaaaacaagg agtcgaccct tctttgcggg gtcaagacct ttctttcgaa gaaatcctcg
     3061 agcttagccg tgttttcacg ggataaaaat ggtattattt taacaatgaa acgcaaaagc
     3121 ctgcttctta tttttctcgc ggcgatattg ttgaccgcgt ttcttggatc ccccgccatt
     3181 gtttcggctc aagacgcaga acaaacggag tcttcatcgg aaagaacttc cgggggccgg
     3241 gtcggtaccg gagaagaaca atcaggctct ttaaattgca ctttatccct tttgagcctc
     3301 aatctcacga acatacttac ctgtcttctt accaactttg ttattgaaat cgttagagcg
     3361 tttgt
//
LOCUS       DAOPQT010000055         3343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_30953_length_3343_cov_3.402676, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000055 DAOPQT010000000
VERSION     DAOPQT010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30953_length_3343_cov_3.402676"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PKG74_01870"
     CDS             complement(<1..699)
                     /locus_tag="PKG74_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90059.1"
                     /translation="MSILIKGANILDGNGNPPRKADILVLKDKISAIGDFRGARPAET
                     VIDGLGSYVAPGFIDVDTDSDHYLTLFSNPGQEDFLKQGVTTIFGGMCGYSLAPLVYG
                     SMESVREWSESERLNVDWRTTKEFLSSLKRMDLGVNFGTLSGHLTMRQDIVKKPRALS
                     EKETKILGTLLEQSLEGGSFGMSSSLGYAPGKDIENEEIIELLKVVKAGNGVYSTHLR
                     DYGEDLLKSVEESVA"
     gene            complement(721..1803)
                     /locus_tag="PKG74_01875"
     CDS             complement(721..1803)
                     /locus_tag="PKG74_01875"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOB90060.1"
                     /translation="MLPEVIRIQAVRVLGVTIIAFVAALLLTPPLFKFLKKFGIKKQI
                     RSAESAPIFAKLHAKKEGTPTMGGILIWASVLGLAILFALLNHFFDGFFAYLNFVNRA
                     ETYLPLAAMFIAASLGFIDDLLGALRIGPNGGGMTTKHKFVMYCLVAAIGAWWFYSKL
                     GCLDNVENACILRIPFFGNFDVGLWYIPIFMLVVVASAFSADLTDGLDGLLGGVSLFI
                     FGGLMVVAFLIRRYNLAVMIGAIIGSLLAFLWNNINPAKFFMGDTGSMALGITMGVIA
                     MQTDSALFLPIIALPLVLETLSVIVQTISKKFFHRKIFLSTPIHHHFEAVGMPEAQIT
                     MRFWIIGFIGVGLGVILFVFYLFGLI"
     gene            complement(1784..2701)
                     /locus_tag="PKG74_01880"
     CDS             complement(1784..2701)
                     /locus_tag="PKG74_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOB90061.1"
                     /translation="MKKKTETNQGIKPHFFILLAAALLLTLMNQRLLSGKAVFGDSGK
                     LSGQAIGYSEVQDRAELPQALVLNEKLNAVAAEVVDLQTKRNLLNYNGGQNWPAASLT
                     KLMAAVVIEEFLDPGETINIPWTGPEDGVILPGEAYSVGDLEKIMLMSSSNKAAYSFE
                     KHSPEGRFVEMMNEKAKDIGLMDTNFTDASGLSESSRTSGDDLVKLVQYIWENHRRVF
                     TINRDPKGTITEIKSGQMKEISATHQFAGRPDFLGGKTGFTASAQGNLISIFSVENHP
                     ILILVLGTESRFEESEKILNFVTDASRSN"
     gene            complement(2667..3236)
                     /locus_tag="PKG74_01885"
     CDS             complement(2667..3236)
                     /locus_tag="PKG74_01885"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOB90062.1"
                     /translation="MPETEKQLEAKIEACLFVYGEPVKVANLAKMIRAEESETRKAID
                     RLKSRLDSEESGLVLIESDDKIQLVTKPVFGKIIEEIVKAEIKEDLTPAAQETLSIVA
                     YLGPISRPEIDYVRGVNSSFILRSLMVRGLVERSPSARRLSSFDYRLSFDALKQLGVS
                     EESELPEYERYREISKIFNEKENRNESGD"
BASE COUNT          805 a          851 c          865 g          822 t
ORIGIN      
        1 ggccacactc tcttcgacgg atttcaaaag atcttccccg taatcccgga ggtgggtcga
       61 atagactccg tttccggctt tgacgacttt caaaagctcg atgatctctt cgttttcgat
      121 gtctttcccg ggagcgtatc cgagactcga agacattccg aaagatccgc cttcgaggct
      181 ttgttcaagg agcgttccca aaatcttcgt ttctttttcg cttaaggctc gcggtttttt
      241 aacgatatcc tgacgcatgg tgaggtgtcc ggagagcgtt ccgaaattga cgccgaggtc
      301 catgcgcttc aggctcgaca agaattcttt ggttgtccgc cagtcgacgt taaggcgctc
      361 gctttccgac cactcccgca ccgattccat ggagccgtag acaagcggag cgagcgagta
      421 gccgcacata cctccgaaaa tggtggttac gccttgtttc aggaaatctt cctgtccggg
      481 attcgagaaa agcgttaaat agtggtcgga gtcggtgtcg acgtcgatga atccgggcgc
      541 gacgtaagat ccgagaccgt cgataacggt ttccgccggt cttgcccccc gaaaatcgcc
      601 gatggccgaa atcttgtcct tcaaaacgag aatatccgcc tttcgcggcg gattgccgtt
      661 gccgtcgagg atgttcgcgc ctttgatcag gatgctcatg atttaattat agcgcgtccg
      721 tcagattaac ccgaaaagat agaacacgaa gaggatcact ccgagtccga cgccgatgaa
      781 tccgatgatc cagaaacgca tggtgatttg cgcctcgggc atgcccacgg cttcgaaatg
      841 gtgatgaatc ggagtggaaa gaaatatttt ccggtggaaa aacttcttgc ttatcgtctg
      901 gacgatcacc gataaggttt cgagaaccaa gggcaaagcg atgatcggca aaaacagagc
      961 cgaatcggtc tgcatggcga tcactcccat ggtgatgccc aaggccatgg agccggtatc
     1021 tcccatgaag aatttcgccg gattgatgtt gttccataag aaagcgagaa gcgacccgat
     1081 gatggcgccg atcatcaccg ccaggttgta gcggcgaata aggaaagcga ccaccatcag
     1141 tcccccgaaa ataaacaaag aaactccgcc gagaagtccg tcgaggccgt cggtcaaatc
     1201 ggccgaaaaa gcgctcgcca cgacaaccag catgaatatc ggaatatacc aaagtccgac
     1261 gtcgaaattc ccgaagaaag gaatgcgaag gatacaggcg ttttccacgt tgtcgagaca
     1321 gccgagcttg gaatagaacc accaggcgcc gatcgcggcc accaaacagt acatgacgaa
     1381 tttgtgtttg gtcgtcattc ctccgccatt cggaccgatg cgaagcgccc ccagaagatc
     1441 gtcgatgaat ccgagactgg cggcgatgaa catggcggcg agcggtaaat aggtttcggc
     1501 gcggttcacg aaattcaaat aagcgaaaaa cccgtcgaag aaatggttga gaagggcgaa
     1561 gagaatggcg aggcccaaaa ccgaagccca gatcagaatg ccgcccatgg tcggcgttcc
     1621 ttctttcttg gcgtgaagtt tggcgaaaat cggagccgat tcggccgagc ggatttgttt
     1681 tttgatgccg aattttttca ggaatttgaa aagcggcggc gtaagcaaaa gggcggccac
     1741 gaaagcgatg atggtgacgc ccaaaacgcg aaccgcctgt atcctaatta cttctggaag
     1801 catcggtgac gaagtttaat attttttcgc tttcctcgaa tcggctctcg gttccgagaa
     1861 cgaggatcaa gattggatgg ttttcgacgg agaatatgga aatcagatta ccttgggccg
     1921 aggccgtgaa tccggttttg ccgcccaaga agtccggtcg tccggcgaac tggtgcgtgg
     1981 ccgagatctc tttcatctgt ccgcttttga tttcggtgat cgttcctttc ggatcgcggt
     2041 tgatcgtaaa aacgcgacga tggttttccc aaatatattg tactagcttc acgagatcgt
     2101 cgccagacgt ccggctcgat tcggaaagcc cggaggcgtc ggtaaaattg gtgtccatca
     2161 gcccgatatc tttggctttc tcgttcatca tctccacgaa tcgtccttcc ggagaatgct
     2221 tctcaaaaga gtaagccgcc ttgttcgacg aggacatgag catgattttt tcgaggtctc
     2281 cgacgctgta ggcttctccg ggtaaaataa ctccgtcttc cggcccggtc caaggaatat
     2341 tgatcgtctc tccgggatca aggaattcct cgatgacgac ggccgccatc agtttggtca
     2401 aagacgccgc aggccaattc tgtcccccgt tgtaattcaa aagattcctt ttagtttgaa
     2461 gatcaacgac ttcggccgcc accgcgttca gtttttcgtt caagacgagc gcctgcggaa
     2521 gttcggcccg atcttggact tcggagtaac cgatggcttg tcccgaaagc ttgccgctgt
     2581 ccccgaaaac cgcttttccg gacaaaagcc gttgattcat taaagtgagc agcaaggccg
     2641 cggcgagcag gataaagaaa tgcggcttaa tcccctgatt cgtttctgtt ttctttttca
     2701 ttgaaaattt tgctgatttc ccgataacgt tcgtattccg gcaattccga ttcttcggaa
     2761 acgccgagct gcttcaaagc gtcgaatgac aaccggtaat cgaaagaaga aagccttctc
     2821 gcgctcggac ttcgttcgac aagcccgcgc accatcaagc ttcgcaggat aaagctcgaa
     2881 ttgactcctc tcacgtaatc gatttccggc cgcgaaatcg gtccgagata agcgacaatg
     2941 gataaagttt cctgcgccgc cggagtcaaa tcttccttga tctcggcttt gactatttct
     3001 tcgattattt tcccgaaaac cggtttggtg acgagctgta ttttgtcgtc ggactcgatc
     3061 aaaacaagtc cggattcttc ggagtccaaa cgggatttca gacgatcgat cgcctttctt
     3121 gtttccgatt cttcggctcg gatcattttc gcgagattcg ctactttcac cggctctccg
     3181 tagacgaaaa gacaagcttc gattttcgct tccaattgtt tttcggtttc aggcatgcgg
     3241 cgaaatgatt atttcttcga attgtccgga ttgttcgatc ttgatggcgt tgtctttcag
     3301 caaatggaga agggccagga aaagaatgac gatttcttga cgc
//
LOCUS       DAOPQT010000056         3321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_31231_length_3321_cov_3.075628, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000056 DAOPQT010000000
VERSION     DAOPQT010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31231_length_3321_cov_3.075628"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PKG74_01890"
     CDS             complement(<1..1094)
                     /locus_tag="PKG74_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOB90063.1"
                     /translation="MKFKFLNRLGDALKAFSGFSGRLPFSLAGWSTSLRRPGRGVDVD
                     PMGNYAGWVYAALSKRAKRIAAIQLRLFELNRSQDVEEIFDHELISLLYRANPIQSKY
                     QFFYTIEMMIGIFGSSPVFKDRAGGKKIMYLWPLRPDLLKAIPDAQGGISSYEYRVDG
                     RLQKFAASDIILNNEPNPQNLLSGFSALSAAGLEIDADLQAALWNKYLLENFSEPGGV
                     LTTEQAIDDKSFERLKSEWNKRHAGPTNAGRWALLEKGLTATPIGRSPKEMDLVETRK
                     FNRNAITTILGVPMSMMTSEDVNLANAEVGERIFAKDTIDPEMNLIVSTFNEFLVPEF
                     GDNLELDYVSPIPEDMVQKVNVASADEGRWL"
     gene            complement(1223..1597)
                     /locus_tag="PKG74_01895"
     CDS             complement(1223..1597)
                     /locus_tag="PKG74_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90064.1"
                     /translation="MSKNILEKLGEGMRKRFEAGADFDHPPVQTEEEKKRAADLMIAK
                     KGVIDRAREYSKTPPAERENKKYLLAEIQEIVDLINDPFVLVIDQADGAPYSITFNPK
                     SHKAIRNFKKPRPIGKKKEIES"
     gene            complement(1629..1859)
                     /locus_tag="PKG74_01900"
     CDS             complement(1629..1859)
                     /locus_tag="PKG74_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90065.1"
                     /translation="MITLSCQACHGTIARDIELEVGSPTTIVLKMMIKCSHCGHPNRI
                     TIRTKLEKEILIDGRILEPGKGESGESIRSLS"
     gene            complement(1856..3082)
                     /locus_tag="PKG74_01905"
     CDS             complement(1856..3082)
                     /locus_tag="PKG74_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HOB90066.1"
                     /translation="MRKTLHHLLEIPDPIQKNRARLEVSELYLRIRESKARVICLEGG
                     VRSGKTFSIAQYLNERLQTPKPAGEKIEIVRKTMPALKATAMEDFFGIMGDLGLYDQN
                     KHNKTDQIYREGKNSVGFFPVDDQQKLRGRKRDILWINEANELTYEEFKQLALRTTRL
                     IILDFNPVEEDHWIVEKIFTRDDVEIIHSDYRCNPFLPPEIVAEIEMMRDADPNYWRV
                     FGLGLRPIKGTRIYSHYTLVDEFPKNPDQIIYGIDFGFNNPSAVVRIGIEERIHTWDE
                     LLYKTHLTNPELIEELIRLRDAGLITESMQGYADTAEPARIEEINRAGFNVKPADKAV
                     VPGIDFIKGRPLRFTKRSLNLIDEAKDYSWKTTKDGKILDEPVKLKDHLMDAGRYGEY
                     THGLSAAEGSARIRIL"
     gene            complement(3015..>3321)
                     /locus_tag="PKG74_01910"
     CDS             complement(3015..>3321)
                     /locus_tag="PKG74_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90067.1"
                     /translation="TLSEWKLREELWSIRDQYLIVFKKHTADIISALAKRAKRTGEAF
                     HSLSFLKVVEGFTEKTGIDLTSKGKKITGFEVVVRHAKNSPSSSRDSGSDSKESSAA"
BASE COUNT          709 a          850 c          859 g          903 t
ORIGIN      
        1 agccatcggc cctcgtcggc ggaagcgaca ttcacttttt ggaccatgtc ctcggggatc
       61 ggggagacat aatcgagttc gagattgtcc ccaaattccg gaacgagaaa ttcattgaaa
      121 gttgagacga tcaagttcat ctccggatcg atcgtatctt tggcaaagat cctttcgccg
      181 acttcggcgt tggcgagatt tacatcctcg gaagtcatca tcgacatcgg aacgccgagg
      241 atcgtggtga tcgcgttgcg gttaaatttg cgagtctcaa cgagatccat ctctttcgga
      301 gatctgccga tcggagtcgc ggtcaagcct ttttccaaaa gcgcccatcg gccggcgttc
      361 gtcgggccgg catggcgttt attccattca cttttcagcc gctcgaaaga cttgtcgtcg
      421 atcgcttgtt cggtcgtcaa tacgccgccc ggttcggaga agttttccaa aagatattta
      481 ttccacaatg cggcttgaag gtccgcatcg atctcaagac cggcggccga taaagcggaa
      541 aatccgctca ataaattttg cggattcggt tcgttgttga ggatgatatc ggaggccgcg
      601 aatttttgga gacggccgtc aacccggtat tcgtaagacg agatccctcc ttgcgcgtcc
      661 gggatcgctt tcagaaggtc cggacgaagc ggccaaagat acatgatttt tttgccgccg
      721 gcgcggtcct tgaaaaccgg agatgatccg aagatcccga tcatcatctc gatcgtgtag
      781 aagaattgat atttactttg aatcggattc gcccggtaaa gaagcgagat caattcatgg
      841 tcaaaaattt cctcgacatc ttgagatctg tttaattcaa agagtcggag ttggatcgcg
      901 gcgatccttt tggccctttt tgaaagcgcg gcatataccc agccggcata atttcccatc
      961 ggatcgacat cgactccgcg gcccggccgt cggagactgg tcgaccatcc ggcaagagaa
     1021 aatggaagcc ttccggagaa gccggagaag gctttgagag catcgccgag acgattcaga
     1081 aatttgaatt tcatatcctt ttttatttat gaaaatcggg ccgtctccgg cgatccatag
     1141 atcggccatt gatcggcccg taggctttcg atattgaatg cttatattat atcacaaaac
     1201 gaaatccgtg tcatccactc cgtcaagact cgatctcttt tttctttccg atcggccgtg
     1261 gctttttgaa atttctgatc gctttgtgcg atttcggatt gaaggtgatg gagtaaggag
     1321 cgccatccgc ttgatcgatg acgaggacga atggatcgtt gatcaaatcg acgatctctt
     1381 gaatttcggc caagagatat tttttatttt cgcgttcggc cggcggagtt ttgctgtatt
     1441 ctctcgcccg atcgatcacg cccttctttg cgatcataag atcggccgct cgcttttttt
     1501 cttcttcagt ttggaccggc ggatgatcga aatcggctcc ggcttcaaat ctttttctca
     1561 tcccttcgcc caatttttcg agaatgtttt ttgacatgac ttttttatat tattatattt
     1621 ttttgaaatt aagacaagga ccggatcgac tctcccgatt ctcctttccc gggttcgaga
     1681 atccggccgt cgatcaagat ctctttttcc agtttggtcc ggatggtgat ccgattcgga
     1741 tggccgcaat gcgagcattt tatcatcatt ttcaagacga tggtcgtcgg agatccgacc
     1801 tcgagttcga tgtctcgggc gatcgttccg tggcatgcct gacatgaaag agtaatcata
     1861 aaattcggat ccttgccgat ccctcggcgg ccgagaggcc atgcgtatat tcgccgtagc
     1921 gcccggcgtc catgaggtga tctttcaact tcaccggctc gtcgaggatc tttccgtcct
     1981 tcgtggtctt ccacgaatag tccttcgcct cgtcgatgag attgagagac cgcttcgtga
     2041 atcggagcgg ccggcctttg atgaagtcga tccccggcac gaccgccttg tccgccggct
     2101 tcacgttgaa tcccgcacga ttgatctcct cgatgcgtgc cggctccgcg gtgtcggcgt
     2161 agccctgcat cgattcggtg atgagaccgg cgtcccggag ccggatcaat tcctcgatga
     2221 gttccgggtt cgtgaggtgc gtcttgtaga ggagttcgtc ccatgtatgg atccgctcct
     2281 cgatcccgat ccggacgacc gcgctcgggt tgttgaagcc gaagtcgatg ccgtaaatga
     2341 tctgatccgg attcttcggg aattcatcga cgagcgtata gtgcgaatag atccgggttc
     2401 cttttatcgg ccgaagcccg aggccgaaaa cgcgccagta gttcggatct gcgtcgcgca
     2461 tcatctcgat ctcggcaacg atttccggcg gcaaaaacgg attacagcga taatcggaat
     2521 gaatgatctc gacatcgtcg cgcgtgaaga tcttttcgac gatccaatgg tcctcctcga
     2581 ccgggttgaa gtcgagaatt atcaagcgcg tcgtgcggag agcgagttgc ttgaattcct
     2641 cgtaggtgag ttcgttcgct tcattgatcc ataggatgtc gcgtttgcgg ccgcggagtt
     2701 tttgctgatc gtcgaccggg aaaaatccga ctgaattttt cccttcgcga tagatctgat
     2761 ctgttttatt atgcttgttt tgatcataaa gaccgagatc tcccatgatg ccgaaaaaat
     2821 cctccatcgc cgtcgccttc aaggccggca tcgtctttct gacaatttca attttctccc
     2881 cggccggctt cggcgtttgg agccgctcgt tgagatattg agcgatcgaa aatgttttcc
     2941 cggaccggac gccgccctcc aaacagatga cccgggcctt gctctcccgg atccggagat
     3001 aaagttcgga gacttcaagc cgcgctcgat tcttttgaat cggatccgga atctcgagaa
     3061 gatgatggag agtttttcgc atgtcggacc acgacctcga aaccggtgat cttttttcct
     3121 ttggaagtga gatcaatgcc ggttttttcc gtgaagcctt ccacgacctt caagaaagag
     3181 agggaatgaa aagcctcgcc ggtccgcttt gctcgtttgg cgagcgcgga aattatgtcg
     3241 gccgtgtgct ttttgaaaac gatgagatat tgatcccgga tcgaccataa ttcctcgcgg
     3301 agtttccact ccgaaagagt c
//
LOCUS       DAOPQT010000057         3306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_31419_length_3306_cov_4.067979, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000057 DAOPQT010000000
VERSION     DAOPQT010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31419_length_3306_cov_4.067979"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PKG74_01915"
     CDS             complement(<1..835)
                     /locus_tag="PKG74_01915"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOB90068.1"
                     /translation="MTSPNIVILSNNVGRKLASSIAERLFQKTHSVEVGRFNDGEIRI
                     EIEDSLRGEDVYIIAPTPAPADNIMEAVLIADAARRSSAGKIVLVLPYLGYGRQDRKD
                     KPRVPLSAKVICDMVSRYGPNHVLVLDAHSEQSLGFFSDKVGVDHLFGSYVGIPYIRE
                     NIPKPFMVASPDAGGVKRARAYADRLGHQNIAIFTKSRPEPGKIGGVSINSDVNGQNI
                     VFVDDMIDTGGTIIANAEAAVKAGAKDIYVFATHALLSKKAVPNLNKSPVKRIAITDS
                     ID"
     gene            complement(933..1121)
                     /locus_tag="PKG74_01920"
     CDS             complement(933..1121)
                     /locus_tag="PKG74_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90069.1"
                     /translation="MRRFRGLDRIRKNPESFQIVLDGMVVLIVVALDPKTPWRQILGV
                     MLLVSAFVSLRRQHAKHR"
     gene            complement(1135..1314)
                     /locus_tag="PKG74_01925"
     CDS             complement(1135..1314)
                     /locus_tag="PKG74_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90070.1"
                     /translation="MKRILEFTKRFWDDPRPFQFIFVIFGSAAALRLAEKFWPETLFV
                     ILVVISIISLINTRK"
     gene            1392..2177
                     /locus_tag="PKG74_01930"
     CDS             1392..2177
                     /locus_tag="PKG74_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOB90071.1"
                     /translation="MSKKIWLWIGIAVLLVLGAMAAKTKISGFSPSEAVNCSPAELLS
                     IICLAAILDSMNPCAFSVLLLTMGFLFSLESVERKKIMRIGVIFIAAIFATYLLIGLG
                     LLKAFQIFGVPRFISRFGAVALIAFGLIEIIGEVFPSFPVKLKIPEKAKPAIAKLIEK
                     GSEWAALLLGFLVGLTEFPCTGGPYLVMLSLLHDKATFVAGFKYLLVYNLIFVIPLVV
                     ILFMASDKGLIQKIDGWRKSSAGKFKLFSAVALMVLGILLLVF"
     gene            2214..2714
                     /locus_tag="PKG74_01935"
     CDS             2214..2714
                     /locus_tag="PKG74_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90072.1"
                     /translation="MKEKAASFEEVMKKVRDLEAGGSLDLSSEEDLSIAIMNLISLEE
                     HFYFTAEKTGKKEYFDMLDEVREMRKKLLAKMIDRKEGESWCIAKHLLAASMRLIEVG
                     TKAQTDKKPEEAKEVFDYAYKLYSMFWAIRLKVIDADGLKEIAKKDEPWKLEDIVDKL
                     VDCCGE"
     gene            2716..3084
                     /locus_tag="PKG74_01940"
     CDS             2716..3084
                     /locus_tag="PKG74_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90073.1"
                     /translation="MDKKQFWPTIFILIIVIGAIILIESFFPKDPDLEYADFAKCLTT
                     QGWIMYGTAYCPHCIDQKAMFKDAFRYVNYVACDIDPEKCVEKDIYEVPTWTSFSGER
                     LSGIQTLEELSRLSGCVLPR"
     gene            3089..>3306
                     /locus_tag="PKG74_01945"
     CDS             3089..>3306
                     /locus_tag="PKG74_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90074.1"
                     /translation="MKISNFNELAVNDLRKAGLLMAEAGLQAIDTKEAIKRAVFLRDE
                     DLIIKDLKIPLSGFGRIFMVGVGKCALDA"
BASE COUNT          784 a          808 c          861 g          853 t
ORIGIN      
        1 ggtcgatcga atcggtgatc gcgatgcgtt tgaccgggct tttgttaagg tttggcaccg
       61 ctttcttgga gagtaaggcg tgagtcgcga aaacgtagat gtctttggca ccggctttca
      121 cggccgcttc ggcgttggcg ataatcgttc cgccggtgtc gatcatgtcg tccacaaaaa
      181 cgatgttctg gccgttgaca tcgctgttga tggaaacccc gccgattttt cccggctccg
      241 gtcgggactt ggtgaagatg gcgatgttct gatggccgag acggtcggcg taagcgcgtg
      301 cccgcttgac ccctccggca tccggagaag cgaccatgaa cggcttggga atgttttctc
      361 ggatataggg gatgccgaca taagatccga agaggtgatc aactcccacc ttgtccgaga
      421 aaaagccgag actttgttcc gagtgcgcgt ccaaaaccaa cacgtggttt ggaccgtacc
      481 gactgaccat gtcgcagata actttggcgc taagcggcac gcgaggcttg tccttgcggt
      541 cttggcggcc gtatccgaga tacggcagaa caaggacgat ttttccggcg gacgatcttc
      601 gggccgcgtc cgcaatgagc acggcctcca taatgttgtc cgccggagcc ggtgttgggg
      661 cgataatgta gacgtcttcg ccgcgcaagc tgtcttcgat ttctattctg atctcaccgt
      721 cgttgaagcg gcccacttcg acagaatgcg ttttctggaa aagcctttcg gcgatgctcg
      781 aggcaagctt cctgcccacg ttgttggaca ggataacgat gttcggactc gtcattgaaa
      841 agacctcctt tgggatttat ccgaatccac cctatttaaa gcttaattcc gggacgccgt
      901 caaataaaaa aaccggcccc gaaacggagc cgtcagcgat gctttgcgtg ttgtcttctc
      961 aaagaaacaa aagcggaaac aaggagcata acgccgagaa tctgtctcca tggcgttttg
     1021 gggtcgagag cgacgactat gagaacaacc attccgtcga gaacgatctg gaacgattcg
     1081 ggattctttc gaatccgatc gaggcctcgg aatcttcgca tttccgttct cctttcattt
     1141 tcttgtgttt attaaagata tgatggagat gacaaccagg atgacgaaga gagtttccgg
     1201 ccagaatttc tccgcgagcc tcaaggcggc agcggaaccg aaaatgacga atatgaattg
     1261 gaaaggtcga ggatcgtccc aaaaccgctt cgtgaactcc aaaatccgct tcatctccat
     1321 tctcctttct tttcaatgat cttccggaat attcgctttt tattagtcga gagtcaagct
     1381 acaatgattt tatgtccaaa aaaatctggc tttggatcgg aatcgcggtt ttgctcgttt
     1441 tgggagcgat ggccgcgaaa accaagattt ccggtttttc tccctccgaa gccgtcaatt
     1501 gttctccggc cgagcttctc tccataatct gtttggccgc cattcttgat tcgatgaatc
     1561 cctgcgcttt ttccgtgctg ctcttgacta tgggattcct tttcagcctc gagtccgtcg
     1621 aaaggaaaaa aatcatgagg atcggcgtca ttttcatcgc cgccattttc gcgacctatc
     1681 ttctaatcgg cctcgggctc ttaaaagcgt tccagatttt cggagtgccg cgcttcatct
     1741 cccggttcgg cgccgtcgct ttgatcgctt tcggactgat cgagatcatc ggagaagttt
     1801 ttccgtcttt ccccgtaaaa ctgaaaatcc ccgagaaagc caaacccgcc atcgccaaac
     1861 tcatcgagaa aggatcggaa tgggcagctt tgcttctcgg atttctcgtc ggactgaccg
     1921 agtttccctg caccggcgga ccgtatctcg tgatgctttc tttacttcat gacaaggcga
     1981 ctttcgtggc cggattcaaa tatcttctcg tttacaacct tattttcgtc attccgctcg
     2041 tcgttattct ttttatggct tcggataaag gtttgatcca aaaaatagac ggttggcgca
     2101 aatctagcgc cggaaaattc aagctttttt cggccgtcgc cctgatggtt cttggtattc
     2161 tgcttcttgt tttctgattt tatggtttgc cgaaaatagg ctaaaatacg tttatgaaag
     2221 agaaagccgc gagtttcgaa gaagtcatga aaaaagtccg ggatttggaa gccggaggca
     2281 gccttgactt gtcttcggag gaagacttga gtatcgccat catgaatttg atcagcctcg
     2341 aggagcattt ttatttcacg gcggagaaaa ccggcaaaaa agagtatttc gatatgcttg
     2401 acgaagttcg ggaaatgcgg aaaaagcttc tcgccaagat gatcgaccgc aaggaaggcg
     2461 agtcctggtg catcgccaag cacttgctcg cagccagcat gcgtttgatc gaagtcggca
     2521 ccaaggccca gacggataaa aagcccgagg aggccaaaga ggttttcgat tacgcctaca
     2581 aactttattc gatgttttgg gcgatccgtt tgaaggtgat cgatgcggac ggactgaaag
     2641 aaatcgcgaa gaaagacgag ccctggaagc tcgaggatat cgtcgacaag ctcgtggatt
     2701 gttgcggaga ataaaatgga caaaaaacaa ttctggccga caatttttat tctgatcatc
     2761 gtcatcggcg cgatcatttt gatcgagagt ttttttccga aagatccgga tttggaatat
     2821 gccgatttcg cgaaatgcct gacgacgcag ggttggataa tgtacggcac ggcgtattgc
     2881 ccgcactgca tcgaccaaaa agcgatgttc aaagacgctt tccgctacgt caattacgtc
     2941 gcctgcgaca tcgatccgga aaaatgcgtc gaaaaggaca tctacgaagt gccgacctgg
     3001 acgagttttt ccggggaacg gctttccggg atccagacat tggaagagct cagccgcttg
     3061 agcggctgcg tattgccgcg ataaaccagt gaaaatatca aacttcaacg agcttgccgt
     3121 aaacgatttg agaaaagccg ggcttttgat ggccgaagcc gggctacaag cgatcgacac
     3181 caaagaggcg atcaagcgag ccgtgtttct gcgtgacgag gacttgatca ttaaagatct
     3241 gaagattccg ctttccggtt ttggccggat ttttatggtt ggcgtcggca aatgcgccct
     3301 cgatgc
//
LOCUS       DAOPQT010000058         3301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_31482_length_3301_cov_2.702095, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000058 DAOPQT010000000
VERSION     DAOPQT010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31482_length_3301_cov_2.702095"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKG74_01950"
     CDS             complement(<1..346)
                     /locus_tag="PKG74_01950"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HOB90075.1"
                     /translation="MNKEAIIVAVVVLYSFILAGIVWGIMPPMMASHWNVAGEVDGFA
                     PKALALLAMPVLGLALAGLFWLLPRLDPLKENIVKFEDVYKKTVVAIFAFLFYVYILT
                     IAWNLGYSFSLPK"
     gene            370..1428
                     /gene="tsaD"
                     /locus_tag="PKG74_01955"
     CDS             370..1428
                     /gene="tsaD"
                     /locus_tag="PKG74_01955"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019375564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOB90076.1"
                     /translation="MRLLAVETSCDETALALVECSEDLENPRFKVLRSVVASQIPLHR
                     RFGGVVPSLAKREHLKNLPIIFRKIGGESLRDKTDAVVVTVGPGLEPALWTGIEFAKS
                     LAKRWKKPLIGASHLQGHLYSFLLEPQENLEDVFPSAALVVSGGHTILLLLQSLVKYK
                     KLGETRDDAAGEAFDKAARLLRLPYPGGPEIEKAAKKGNPKAVAFPRPMIHDKNYDFS
                     FSGLKTSLLYYLKQSFKADWKEFNKNYGQNKIKLENLNDVAASFEQALVDSLVYKTVK
                     AAKEFKVKSIGVSGGVAANSLLRNSLRKESKKLDIAFLSASKRWNTDNAAMIAVAGYI
                     NFLNRKRLKIEAQPNLGL"
     gene            1439..>3301
                     /locus_tag="PKG74_01960"
     CDS             1439..>3301
                     /locus_tag="PKG74_01960"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOB90077.1"
                     /translation="MIELKSLKEDEIYELWEKSGYFNPDNLPRKNRKPYTIIMPPPNA
                     NGALHLGHALGIAIEDAFIRFYRLMGRKALWVPGADHAGFETQVVFEKKLEKEGRNRF
                     DIPREELWREMYAFTQENRKTMENQLRKLGASCDWSRKKFTLDEDIIRETQRSFVELH
                     KQGLIYRGSRLVNWCPKHQTAFSELEVKYVERVDPLYYVKYGPLSVATARPETIFGDT
                     GLAVNPKDKRYQDYIGKDIEFDGLLGKVKLPVVADESVDPDFGTGVVKVTPAHDMTDF
                     ETGLKHDLPAKSVIDRYGKMNEFTGPYKGMKVAEARAAVVVDLEKAGRIEKIDENYRH
                     SVATCYKCGSLIEPAILPQWYVKMKPLAAKGLEAVKSGKIKFVTEHFEKIYIHWLENI
                     HDWNISRQIVWGIRIPAWFCAKCGEAVVSEKIPERCPKCNGSILEQDPDVLDTWFSSG
                     QWPFLALGFPKSRDFKTYYPTDLMETGYDLVFFWVSRMIMLGLWRTKKPPFRNVYFHG
                     MVRDKDRQKMSKSKGNVIDPLGVAEIYGADAVRMALIVGNTPGLDPVVSEDKIRAYRN
                     FATKIRNAAKFVLTYIEPDSKIKPRFSKKDLDNLRELKIFKKEIDTVLLKEKNEM"
BASE COUNT          930 a          875 c          806 g          690 t
ORIGIN      
        1 acttcggcag gctgaaggaa tagccgagat tccaggctat ggtgagaata tagacgtaaa
       61 aaagaaaagc gaaaatcgcg accacggttt ttttgtaaac gtcttcgaac ttgacgatat
      121 tctctttcaa aggatcgagc cgcggcaaaa gccagaataa tccggcgaga gcgagaccca
      181 gaaccggcat cgcgagcaga gccaaagcct tgggtgcgaa tccgtcgacc tcgcccgcca
      241 cattccaatg gctcgccatc atcggcggca ttatccccca aacgatcccc gcgagaataa
      301 aagaataaag caccaccacc gccactatta ttgcttcttt gttcatgact atattatata
      361 ttgaaaggca tgagattatt agccgtcgaa acaagctgcg acgaaacagc cctggccctg
      421 gtggagtgtt cggaagattt ggaaaacccg cgcttcaaag tcctccgaag tgtcgtcgct
      481 tcgcaaatac cgcttcatcg gcgattcgga ggcgtggtgc cgagtctcgc caaacgggaa
      541 catctgaaaa acctgccgat aattttccgg aaaatcggcg gcgaatctct cagagacaaa
      601 accgacgccg tggtcgtcac cgttggtccg ggactcgaac cggctttgtg gaccgggatc
      661 gaattcgcta aaagtttggc caagcgctgg aaaaagcctt tgatcggagc gagccatctc
      721 cagggtcatc tctacagttt tcttctcgag ccgcaagaaa acctcgaaga tgtttttccg
      781 tccgcggctt tggtcgtctc cggcggccac accattcttc ttctactcca gagcctcgtc
      841 aaatacaaaa aactcggaga aaccagggat gatgccgcgg gcgaagcctt tgataaagcc
      901 gcgagactgc tccggcttcc ctatcccggc gggccggaaa tagaaaaagc cgccaaaaaa
      961 ggaaacccca aagccgtggc ttttccgcgg ccgatgatcc acgacaaaaa ctacgatttc
     1021 agcttctcgg gactcaaaac cagcctgctt tactatctca agcaaagctt caaagcagac
     1081 tggaaagaat tcaataaaaa ctacggccaa aacaaaataa aattggaaaa tctgaacgac
     1141 gtggccgcga gcttcgaaca agccttggtc gattccctgg tttataaaac cgtgaaagcg
     1201 gccaaagagt tcaaagtaaa atccatcggt gtttccggcg gcgtagccgc caacagcctt
     1261 ttgaggaact ctttaaggaa agaatcgaaa aaactcgaca tcgctttcct gtcggcaagt
     1321 aaaagatgga acacggacaa cgccgccatg atcgccgtcg ccggttatat caattttttg
     1381 aacagaaaaa gactcaaaat tgaagctcaa cccaatctcg gcttataatt cataatatat
     1441 gatcgaactc aaatccctga aagaggacga aatctacgaa ctttgggaaa aatccgggta
     1501 tttcaacccg gacaaccttc cccggaaaaa tcgcaagccc tacacgatta tcatgccgcc
     1561 gccgaatgct aacggcgccc tgcatttggg gcacgctttg ggaatcgcca tcgaagacgc
     1621 ttttatccgt ttttatcgcc tgatgggccg caaagccctt tgggttccgg gcgcggacca
     1681 cgccggattc gaaacccagg tggttttcga gaaaaaactg gaaaaagaag gccgtaaccg
     1741 cttcgatatt ccccgtgaag agctttggcg ggaaatgtac gcctttaccc aggaaaacag
     1801 aaaaaccatg gaaaaccagc tccgcaagct cggtgcttcc tgcgactggt cccggaaaaa
     1861 attcactctc gacgaagaca tcatccggga aacgcaaaga tctttcgtcg aacttcataa
     1921 acaaggattg atctatcgcg gcagccggct ggtgaactgg tgcccgaaac accagaccgc
     1981 tttttcggaa ctggaagtga aatatgtcga gcgcgtcgat cccctctatt acgtcaaata
     2041 cggccccttg agcgtggcca cggcccgacc ggagacgatc ttcggagaca ccggcctcgc
     2101 ggtcaatccc aaagataaac gataccaaga ttatatcggt aaggatatcg agttcgacgg
     2161 cttgcttgga aaagtcaaac tgccggtggt tgccgacgaa tcggttgatc cggatttcgg
     2221 gaccggagtc gtcaaagtga cgccggcgca cgacatgacc gatttcgaaa ccgggttgaa
     2281 gcacgatctt cccgccaaat ccgtcatcga tcgctacgga aaaatgaacg aatttaccgg
     2341 cccctataaa ggaatgaaag tcgccgaggc gagagcggcc gtggtcgttg atctcgaaaa
     2401 agccggccgc atcgaaaaaa tcgacgagaa ttatcgtcac agcgtcgcca cctgctacaa
     2461 atgcggcagt ttgatcgaac ccgctatcct cccccaatgg tacgtcaaaa tgaaacccct
     2521 ggccgcgaaa ggtttggaag cggtcaaatc cggaaaaata aaattcgtca ccgagcactt
     2581 cgaaaaaatc tatatccact ggctcgagaa tatccacgac tggaacattt cgaggcagat
     2641 tgtctggggc atcaggatcc ccgcctggtt ctgcgccaaa tgcggagagg ccgtcgtctc
     2701 cgaaaaaatc ccggagcgct gcccgaaatg caatggttcg attcttgagc aggatccgga
     2761 tgttttggat acctggtttt cttccgggca atggccgttt ctcgctttgg ggttcccgaa
     2821 atcccgcgac ttcaaaacct attacccgac cgatctcatg gaaaccggat acgaccttgt
     2881 tttcttctgg gtgagccgca tgatcatgct tgggctctgg cgaaccaaga aacctccgtt
     2941 ccggaacgtt tatttccacg ggatggtgcg ggataaagac cgccagaaaa tgtcgaagtc
     3001 caagggcaac gttatcgatc ccttgggcgt ggccgagatc tacggagccg acgccgtgag
     3061 aatggcgctt atcgtcggca acactccggg actcgatccg gtcgtttccg aagacaaaat
     3121 acgcgcctac cgcaatttcg cgaccaagat caggaacgcg gccaaattcg tcttgaccta
     3181 tatcgaaccg gactcgaaaa tcaaaccccg tttttccaaa aaagacttgg acaatttgag
     3241 ggaattgaaa atcttcaaaa aagaaatcga taccgttttg ttaaaggaaa aaaacgaaat
     3301 g
//
LOCUS       DAOPQT010000059         3259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_32016_length_3259_cov_3.980649, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000059 DAOPQT010000000
VERSION     DAOPQT010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32016_length_3259_cov_3.980649"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKG74_01965"
     CDS             <1..382
                     /locus_tag="PKG74_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HOB90078.1"
                     /translation="MLKLLDSAIANAKNLGFKPEKLIIKEIRVDGGPMLKRWLPRAQG
                     RATPIQKKRSHVFLKLEESEKVKVSKFVFPEKTSKETSKKPKTEKKPKSEKPKVELEE
                     KSTGLEEPKDKGGLVRRVFRRKSI"
     gene            388..1068
                     /gene="rpsC"
                     /locus_tag="PKG74_01970"
     CDS             388..1068
                     /gene="rpsC"
                     /locus_tag="PKG74_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOB90079.1"
                     /translation="MSQKIKPTSFRLGVLKNWDSRWFADPKNRKFVLEEDSLIRKLIN
                     KKLKEAGIASIEIERTASSCRIIIKASRPGIIIGRGGKGIEDITKLLNREIAKLHKRS
                     KLPATEISLTVMELGRGDVSAQVVAQNIANDLEKRFRFRPTIKKAIEDVMNHREVKGV
                     KIKLSGRLDGAEISRREWLTRGSLPLSSLRADIDYAEATAFATYGAVGIKVWIYKGEV
                     FTNDEKSG"
     gene            1088..1501
                     /gene="rplP"
                     /locus_tag="PKG74_01975"
     CDS             1088..1501
                     /gene="rplP"
                     /locus_tag="PKG74_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOB90080.1"
                     /translation="MLIPKKVKHRKWQKGRSRKRQAETRGLTLSHGSFGLQALEAKYL
                     NSKQIEAARKTLTNFIKREGKIWIRVFPDKPVTQRPPEVTMGGGKGNVDHYVFSVKPG
                     RVIFEMDGVSEATAERALKLAGFKLPFKTRIIKKS"
     gene            1512..1775
                     /gene="rpsQ"
                     /locus_tag="PKG74_01980"
     CDS             1512..1775
                     /gene="rpsQ"
                     /locus_tag="PKG74_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOB90081.1"
                     /translation="MEENNIQKISSRRRFAGVVVSDKMKKTVVVEVAHTRKNPKYQKY
                     YTKNSRFKAEAEDGLYKIGDKVVIEETIPLSKEKHWKVVSKKD"
     gene            1777..2145
                     /gene="rplN"
                     /locus_tag="PKG74_01985"
     CDS             1777..2145
                     /gene="rplN"
                     /locus_tag="PKG74_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOB90082.1"
                     /translation="MIQERSILKVADNSGAKTIRCFRVLGGSRKRYAKIGDIIVASVQ
                     TVEPRKNIKKKDIVKAVIVRTKNALRRKGGLYVRFDDNSAVIVSVKKEPLGTRIFGPI
                     PRELKEKGFEKIVSLAQEIV"
     gene            2150..2455
                     /gene="rplX"
                     /locus_tag="PKG74_01990"
     CDS             2150..2455
                     /gene="rplX"
                     /locus_tag="PKG74_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOB90083.1"
                     /translation="MNIKKNDNVYILKGKDAGKSGKVLKVDSKLERIVVSGINQYKKA
                     VKPKKEGEKGQFVTISRPIDISNAMLVCPKCGKKSRVGYRIEKDKKIRYCKKCKATI"
     gene            2437..2916
                     /gene="rplE"
                     /locus_tag="PKG74_01995"
     CDS             2437..2916
                     /gene="rplE"
                     /locus_tag="PKG74_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOB90084.1"
                     /translation="MQSDYLEKIIVSAGVGKLRLASPQFEEKALPEIMKEFAAITGQK
                     PAITKAKKSIAGFKVRQGEVVGLKAVLRGKRMNDFLSRVVNVVLPRVRDFRGLSPKSI
                     DQNGNLSFGFRDQLVFPEINPETSRVNFGIEVTLVSRLKNKEKAIDFYRSQGIPLAK"
     gene            2926..3111
                     /locus_tag="PKG74_02000"
     CDS             2926..3111
                     /locus_tag="PKG74_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOB90085.1"
                     /translation="MAKESVIARACKKPKFSTREVRRCFRCGRKHGYLRDFGLCRICF
                     REMASKGQIPGVRKASW"
BASE COUNT         1026 a          759 c          854 g          620 t
ORIGIN      
        1 gatgcttaag cttttggatt cggccatcgc caatgccaag aatttggggt tcaagccgga
       61 aaaacttatc atcaaggaaa tccgggtcga cggagggccg atgctgaagc gttggcttcc
      121 gcgagcccaa ggccgggcga cgcccatcca gaaaaagcgc tcccatgttt tcttgaagct
      181 cgaagaatcc gaaaaagtca aagtttccaa attcgttttc ccggaaaaaa cttccaaaga
      241 aacgtcgaaa aaaccgaaga ccgaaaagaa accgaaatcc gaaaagccga aagtcgaatt
      301 ggaggaaaag agcaccggtc tcgaagagcc gaaagacaaa ggcggactcg tgaggcgcgt
      361 ctttagaagg aaatccatat aaaaaacatg tcacaaaaaa taaaaccgac atctttccga
      421 ctgggcgttc tgaagaattg ggattctcgc tggtttgccg accccaaaaa ccggaaattc
      481 gttctcgagg aagacagcct gatccggaaa ttgatcaaca agaaattgaa agaagccggt
      541 atcgcttcga tcgaaataga aagaacagcc agctcttgcc gcattatcat caaagccagc
      601 cggccgggaa tcatcatcgg acggggcggc aaaggcatcg aagacatcac caagcttttg
      661 aacagggaga tagccaaact ccacaaacgg agcaagcttc cggcgacgga aatcagcttg
      721 accgtgatgg agctcggccg gggagacgtt tcggcccagg tggtcgccca gaatatcgcg
      781 aacgatttgg aaaaaagatt ccgcttcaga ccgacgatca agaaagccat cgaggacgtg
      841 atgaaccaca gagaggtcaa gggcgtgaaa atcaagcttt ccggccgttt ggacggagcg
      901 gagatttccc gccgcgaatg gctgaccagg ggcagtttgc cgctatcgag cttgagagcc
      961 gacatcgatt acgccgaagc caccgccttc gccacctacg gagcggtcgg catcaaagtc
     1021 tggatttaca aaggagaagt ctttactaac gacgaaaaat ccggctaaaa tacccatctg
     1081 aacttccatg cttattccca aaaaagtaaa acatcgcaaa tggcagaaag gccggtcgcg
     1141 gaagcggcag gccgagaccc gcggcctgac tttgagccac gggagttttg ggctgcaagc
     1201 cttggaagcc aaatatctga attccaaaca gatcgaagcg gcgagaaaga ctttgaccaa
     1261 tttcatcaaa agggaaggaa agatatggat ccgggttttt ccggacaaac ccgtcaccca
     1321 aaggcctccg gaggtgacca tgggcggagg caagggaaat gtcgaccatt atgttttttc
     1381 ggtcaaaccc ggaagagtga ttttcgagat ggacggagtg agcgaggcaa cggccgaacg
     1441 cgccttgaag ctcgccggat tcaagcttcc gttcaagacg agaatcatca aaaaatccta
     1501 attttaaaaa catggaagaa aacaacattc aaaaaatcag ttctcgccgc agattcgcgg
     1561 gagtcgtcgt ttcggacaaa atgaaaaaga ccgtggtggt cgaggtggcc catacccgca
     1621 aaaatccgaa ataccagaaa tattacacca aaaacagccg tttcaaggcc gaggccgaag
     1681 acggccttta caagatcgga gacaaggtcg tgatcgaaga gaccataccc ctttccaaag
     1741 aaaaacattg gaaagtcgtt tccaaaaaag actaatatga ttcaggaaag atccattttg
     1801 aaagtggcgg acaattccgg agcgaagacc atccggtgtt ttcgggtttt gggcggaagc
     1861 cgcaagcgtt acgccaagat cggcgacatc atcgtcgctt cggtgcagac cgtcgaaccc
     1921 cgcaagaaca tcaagaaaaa ggatatcgtc aaagccgtca tcgtccgcac caaaaacgct
     1981 ttgagaagga aaggcggact ctatgtccgt ttcgacgata actcggcggt gatcgtgagc
     2041 gtcaaaaaag agcctttggg aacgagaatt ttcggaccga tcccgaggga gcttaaggaa
     2101 aaaggattcg agaaaatcgt ttctctggcc caggaaatag tttaagacca tgaacatcaa
     2161 gaaaaacgac aacgtttaca tacttaaggg aaaggatgcc ggcaaatccg gcaaggtcct
     2221 caaagtcgat tccaaactcg agagaatcgt cgtttccgga atcaaccagt acaagaaagc
     2281 ggtcaaaccg aaaaaagaag gcgagaaagg ccagtttgtc acgatttcga ggccgatcga
     2341 tatctcgaac gccatgctcg tctgtcctaa atgcggaaag aagagccggg tcggataccg
     2401 gatcgagaaa gacaaaaaaa taagatactg caaaaaatgc aaagcgacta tttagaaaaa
     2461 atcatcgtct ccgccggcgt cggcaaattg aggcttgcga gcccgcagtt cgaggaaaaa
     2521 gctttgccgg agatcatgaa ggaattcgcc gccatcaccg gacagaaacc ggcgatcacc
     2581 aaagcgaaaa aatccatcgc cggattcaag gttcgccaag gagaagtcgt cggcctgaaa
     2641 gcggttttga ggggaaaaag gatgaacgat ttcctttcga gggtggtgaa cgtcgtcttg
     2701 ccgcgggtga gagacttcag gggattgagc ccgaagagca tcgaccaaaa cggcaatttg
     2761 tctttcgggt tcagggacca gttggttttt cccgaaataa acccggaaac gagccgcgtc
     2821 aatttcggca tcgaagtgac gctcgtcagc cgcctgaaaa ataaggaaaa agctattgat
     2881 ttttataggt cacaagggat accattagca aagtaaaata agaaaatggc caaagaatca
     2941 gtcatagcca gagcgtgcaa aaagccgaaa ttcagcaccc gcgaagtcag gcggtgtttt
     3001 cgctgcggcc gcaagcacgg atatctgcgc gatttcgggc tttgccggat ttgtttccgc
     3061 gaaatggctt ccaaaggcca gattccggga gtgagaaaag cgtcttggta aaatgtatat
     3121 acagactctc gtaaaaatca aaaacgcgaa gctcgccgga aaaaaatctt tgaaggttcc
     3181 tttttcgaaa atggacatgg cggttctcga agctttggtc agagccggct atctcgcttc
     3241 cgtcgagaaa aaaggaagg
//
LOCUS       DAOPQT010000060         3229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_32395_length_3229_cov_2.826717, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000060 DAOPQT010000000
VERSION     DAOPQT010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32395_length_3229_cov_2.826717"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..2406)
                     /locus_tag="PKG74_02005"
     CDS             complement(127..2406)
                     /locus_tag="PKG74_02005"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOB90086.1"
                     /translation="MNKQNFTIKGMRCSSCANIIKNRLENLEGVEKADVNFAADRAEI
                     EYDPEKVSVENMNDDLEKLGYSLFSREITEQSTDHSDHSEHLGIGMSKKQKEQELAAL
                     RNKIKFIFPAAIVVFLYMMWDIAAGFFGFLPKIPIPMDIVNVVLFVLSSVALFWVGQP
                     FIQGVVKFVKYRAANMDTLIGIGALTAYIYSAIVVLLPRIRTILELPETTYFDVTIVV
                     IGFVSLGKYLEAQSRLKTGEAVEKLLGLQAKTALVLREGEEKEVPIAEVMVGDIVIVK
                     PGEKIPVDGKIIEGKTSIDESMITGEPLPIDKFVGDLVVGSTVNKQGAFKFKATKVGK
                     DTMLAQIIKMVEEAQGSKAPIQALADRISAVFVPTVLVIAVFSFVGWLAFGIPDLGFS
                     TALSYAILSLVGVLVIACPCALGLATPTAIIVGVGKGAEYGILVKNAEALEKLSEVDT
                     IVFDKTGTITKGKPEVTDVEVLDNTLKTDDILKYAGSAEKKSEHPLAKAIVAEALRRK
                     ISLVESSEFQALEGVGIKATVQGKNIYIHKPDKTNRDDRLLRLERQGKTAAVVEIDNR
                     RSGIIALSDTLKPEAQEAVNRIHGQGKKTMMMTGDNMETAKYIAGQIGIDEVMADVLP
                     GEKAQRIKDLQSKGRKVAMAGDGINDAPALAQADVGIAMATGTDVAIESSGITLLHGD
                     IKKLAEALELSRQTIKTVKQNLFWAFIYNVIGIPVAAGLLYPFWGIVLNPIFAGLAMA
                     GSSVSVVMNSLRLKARKLS"
     gene            complement(2416..>3229)
                     /locus_tag="PKG74_02010"
     CDS             complement(2416..>3229)
                     /locus_tag="PKG74_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOB90087.1"
                     /translation="LGSMLGLFGEKMKMSPIVASIMVLFVSGAMLVLALQMIGIKAFQ
                     RIRIALPKSFSARLFKNRNKFFEPFVVGFLTFLLPCGFTVVVEGFAVLSGNPLKGFLI
                     MLFFGLGTTIPLFMIGFSSAKFLGKERLSDRFLKIIGLLILFFTIYNLDFQFGISRFV
                     SEKWFAQNQMERIDPTAANVQIIKAVYEGVQDIQPNSFEVKAGQPIRFEVEVKTASYG
                     CMSTIMIPGLWNRPLPLKKDQTLVMEFIPQKPGVYQIACAMGVPRGTIKVVE"
BASE COUNT          737 a          815 c          821 g          856 t
ORIGIN      
        1 ttcttcggct tccggactcg atcgataaga acgcatcagg aaaacaagac cggcgatcaa
       61 aacaagaagg aacaacacgg aaatgatttt tttgttcata tgattattat aatcattttg
      121 tcatctttag gataattttc tcgctttcaa tctcaaggaa ttcatgacca cggagacgct
      181 gcttccggcc atggccaatc cggcgaaaat cggattcaaa acgattcccc agaacggata
      241 gagcagtccg gcggccacgg gaatgccgat gacattgtat atgaaagccc agaacagatt
      301 ctgtttcact gttttgatcg tctgacggga gagctccagg gcttcggcca attttttaat
      361 gtccccatgg agaagggtga ttccgctcga ttcgatggcg acatcggtcc cggtggccat
      421 ggcgattccc acgtccgcct gggccagagc cggtgcgtcg tttattccgt ctccggccat
      481 ggccactttc ctgcctttgc tttgcagatc ttttatcctt tgcgcttttt ctccgggcag
      541 gacatcggcc atcacctcgt cgatgccgat ttgtccggcg atgtatttcg cagtctccat
      601 gttgtctccg gtcatcatca tcgttttttt tccttggccg tggatgcggt taacggcttc
      661 ttgcgcttcc ggtttaaggg tatcgctcaa ggcgatgatt ccggatcgac gattgtctat
      721 ctctacgaca gccgcggttt tcccctggcg ttcgagccga agcagacgat cgtctctatt
      781 cgttttgtcc ggcttgtgga tatagatatt ttttccttgg accgtagctt tgatacccac
      841 gccctcgaga gcttggaact cggagctttc caccaaagat attttccgcc tcaaggcttc
      901 ggcaacaatg gccttggcta aaggatgttc ggattttttc tcggcgcttc cggcgtattt
      961 caagatatcg tccgtcttga gagtattatc cagaacttcg acatccgtca cttcgggttt
     1021 tcctttggtg atggtgccgg ttttgtcgaa aacgatcgtg tccacctcgc tcaacttctc
     1081 gagggcttcc gcgtttttga caaggatgcc gtattccgct cctttgccga cgccgacgat
     1141 gatcgccgtc ggcgtggcca aacccaaggc gcaaggacaa gcgataacca agactcccac
     1201 gagggaaaga atggcatagg acaaggctgt cgagaatccg aggtcgggga taccgaatgc
     1261 gagccagccg acgaaagaga atacggcgat cacgagaacc gtcggaacaa aaacagccga
     1321 gatgcggtcc gccaaagcct ggatcggagc tttcgagccc tgggcttctt ccaccatttt
     1381 gatgatctgg gcgagcatcg tgtctttgcc gaccttggtg gctttgaact tgaaagctcc
     1441 ctgtttgttg acagtcgaac cgacgaccag gtctccgacg aatttgtcga tcggtagcgg
     1501 ttctccggtg atcattgatt cgtcgatcga ggtttttcct tcgattattt ttccgtcaac
     1561 cgggattttt tctccgggtt tgacgatgac gatgtcgccg accataactt cggcgatcgg
     1621 aacttccttc tcttcgcctt cgcgcaaaac caaggcggtt ttagcctgta gcccgagcag
     1681 tttttccacg gcctcgccgg ttttcaatct cgattgcgct tcgagatatt tacccaagga
     1741 tacgaagccg atgacgacga tcgtaacgtc gaaataagtc gtttccggaa gctccaagat
     1801 cgtccttatc cggggtagaa gaacgacgat cgcgctgtaa atataggctg tcaaagcgcc
     1861 gatgccgata agcgtgtcca tgttcgccgc ccgatacttg acgaatttga ccactccttg
     1921 gataaaaggc tggccgaccc aaaaaagcgc caccgaagaa agaacaaaaa ggacaacgtt
     1981 cacgatatcc atcgggatcg gaatcttcgg cagaaatccg aagaaaccag cggcgatatc
     2041 ccacatcata tagaggaaga cgacgatcgc cgccggaaag atgaatttga ttttgttccg
     2101 gagcgccgca agctcttgct ctttttgttt tttactcatt cctattccga ggtgctcgct
     2161 atggtccgaa tgatccgtgc tttgttcggt tatctcgcgg gaaaacaggg aatagccgag
     2221 cttttccaaa tcatcgttca tgttttccac ggagaccttt tcgggatcgt attcgatctc
     2281 cgctcgatcg gccgcgaaat tgacatccgc tttttctact ccttccagat tttccaatct
     2341 gtttttgatg atattggcgc aactcgagca gcgcataccc ttgatcgtga agttttgttt
     2401 attcatggta tttttttatt cgacgacttt tatggtgcct ctcggcacgc ccatggcgca
     2461 ggcgatttgg taaactccgg gtttttgggg aatgaattcc atgacgagcg tctggtcttt
     2521 tttaaggggg agcggccggt tccaaagtcc ggggatcatg atggtcgaca tgcatccgta
     2581 gctcgcggtt ttgacttcga cttcgaatct gatcggctgg ccggccttga cttcgaaaga
     2641 attcggctgg atgtcttgga cgccttcgta aaccgctttg atgatctgga cgttcgcggc
     2701 cgtcggatcg atccgctcca tttgattttg tgcaaaccat ttttccgaaa cgaatcggct
     2761 gatcccgaat tggaaatcga ggttgtagat cgtgaaaaat aggatgagga ggccgatgat
     2821 tttgaggaat cgatcggata aacgctcttt gcccaagaat ttggcgctgg aaaagccgat
     2881 cataaagagg ggaatggttg tgccgaggcc gaagaaaagc attatcaaga atcccttcag
     2941 gggatttccc gacaggacgg cgaatccctc gacgaccacc gtgaacccgc aaggcaagag
     3001 aaaggtcagg aatccgacga caaacggctc gaagaatttg tttcggtttt tgaaaagccg
     3061 cgcggaaaag cttttaggca aggcgatccg tatccgctgg aaagccttga taccaatcat
     3121 ttggagcgcc agaacaagca tcgctccgga gacgaataga accatgatcg aagcgacaat
     3181 cggagacatt ttcatttttt ctccgaataa tccgagcatt gatccgagc
//
LOCUS       DAOPQT010000061         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_32411_length_3227_cov_3.648487, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000061 DAOPQT010000000
VERSION     DAOPQT010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32411_length_3227_cov_3.648487"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..458)
                     /gene="rpsM"
                     /locus_tag="PKG74_02015"
     CDS             complement(75..458)
                     /gene="rpsM"
                     /locus_tag="PKG74_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOB90088.1"
                     /translation="MRLIGINIPDEKRIEVALTYIYGIGPTVSKRILKETKIDPNKRA
                     KNLDQGEVSLLKDFIEKNCKIEGELRQIVRQNVGRLKEIQCYRGTRHARKLPVRGQRT
                     KTNSRTVRGNVRKTAISGKRKVELK"
     gene            complement(463..579)
                     /gene="rpmJ"
                     /locus_tag="PKG74_02020"
     CDS             complement(463..579)
                     /gene="rpmJ"
                     /locus_tag="PKG74_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOB90089.1"
                     /translation="MKVRPSVKKICKDCKIVRRRGRIYVVCKKTPKHKQRQG"
     gene            complement(603..806)
                     /gene="infA"
                     /locus_tag="PKG74_02025"
     CDS             complement(603..806)
                     /gene="infA"
                     /locus_tag="PKG74_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOB90090.1"
                     /translation="MHMIEKGVVLEALPAANFRVELESGKEVLAYLSGRMRQNHIKVL
                     PGDKVTVELSPYDDKRGRVTRRL"
     gene            complement(851..2458)
                     /locus_tag="PKG74_02030"
     CDS             complement(851..2458)
                     /locus_tag="PKG74_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HOB90091.1"
                     /translation="MEKPENKKIYLKKTDSAAAVIQRILNSRESKVILAVPRYSLLGE
                     NGNNFYLIFREASAMGKQVIIESSDEDIVDLALASGFQVINPVFKKVKRAVADIIPVS
                     ARVKPRPTRVPFIREEPAIKTQPEEEKVPETEPIIREYDPEPSPKPASEYRPRKSRNI
                     KIIVFSSGGILFAGLLAYAAVFVLPKAEARLTLVKTRWETPIAITASIKASAETGFTI
                     PGQIFSIDKNNVFSAPASGTKRVEESAEGVITVYNAYSSSKQSIVKGTRFITTGGKIF
                     KTNEAIIIPGAAISEGKIVASSIDVKVTAEKPGSDYNLPAGQSWRIPGFEGTPKFNGF
                     YGESKEAMSGGFVGDVKVPTEADIASLKEKSRQELLAMVKAAEGITVPETLRRIEGAT
                     SYAIVKEEVIDDIDEKGNFGLMLAAETKIMAFDEASVRKVVEEKLQTSGPGEDYEMKS
                     YEIDYGEASVNFDTGKAIIPVTIVSEWSRVFDAEAFKSVVAGKSAEDFSLYVAGDEEI
                     QAAVVTLWPFWVRSIPADFGRVTLTLE"
     gene            complement(2458..>3227)
                     /locus_tag="PKG74_02035"
     CDS             complement(2458..>3227)
                     /locus_tag="PKG74_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90092.1"
                     /translation="AGPAAAGRVGLGVPEIDVLLIDARIVSARVDGSKVLNPIGFPAK
                     RIEVGVAETLASRDTFQKIRQGLPKNGEIIFVSEPTSVKSHLLREESRCKNFVFVNVL
                     RDKTHVCAAKENGDIRPAGDFEWGLDNLLQSFHDDLTVPKDSVAPVLNRYLQNDASPD
                     FLKKFRFVFNKHFSVFEKGVSAAIHNAKMVKPIAFVMCDQIAASAKPFTFGTGAKFAF
                     LPWEKPGDILKREFVQFSSNKNFNRFAKQRIKWLNIN"
BASE COUNT          660 a          889 c          774 g          904 t
ORIGIN      
        1 ccttggcctg cttttcttct ccttcggcga tattctcttg tttcgaaact actttctttt
       61 ttcccatggc aatattactt caattccacc tttcttttgc cgctgatggc ggtttttctc
      121 acgttcccgc ggaccgtccg ggaattggtt ttggtgcgct gtccgcgaac aggcagtttt
      181 ctcgcgtgcc ttgttccgcg gtaacactga atctctttca agcgtccgac gttctgacgg
      241 acaatctgcc tcaattctcc ctcgatcttg cagtttttct cgataaaatc cttcaagagg
      301 ctcacctctc cctgatccaa atttttggct ctcttgttcg gatcgatctt ggtttccttg
      361 agaatccttt tggaaactgt cggtccgatt ccgtaaatat aagtaagcgc cacctcgatg
      421 cgcttttcgt ccggaatatt tatgcctatc aatctcattt aactatcctt gtctctgctt
      481 atgttttgga gtttttttgc aaaccacata aatccgccct cggcggcgga caatcttgca
      541 atccttacaa atttttttca ccgatggtct gactttcata ccgatgatct taacgcggat
      601 ttttaaagcc tccttgtcac gcgccctctt ttgtcgtcgt aaggagacaa ttccaccgtc
      661 actttgtcgc cgggcaaaac cttaatgtga ttctgcctca tgcggccaga tagatacgcg
      721 aggacttctt ttccgctttc caactcaacc cgaaagttcg ccgccggaag agcttcgaga
      781 acaactcctt tttctatcat gtgcatggat tctaccacaa gggaccgccc ggagtcaata
      841 tccgaggccc ttattcgagg gtcagggtga cgcgaccgaa gtcggccgga atgcttctca
      901 cccagaaagg ccaaagcgtg acgaccgccg cctggatctc ctcgtctccg gcgacataaa
      961 ggctaaagtc ttctgctgat tttccggcaa caaccgattt gaatgcttcg gcatcaaaga
     1021 ccctagacca ctccgagacg atggttacgg gaatgatcgc cttgcccgta tcgaaattga
     1081 cgctcgcttc tccgtaatcg atctcgtagc tcttcatttc gtaatcctcg cccggaccgc
     1141 tcgtttggag cttttcctcg accactttgc ggacgctcgc ctcgtcaaag gccatgattt
     1201 tggtttcggc ggcgagcatc aatccgaaat tccctttttc gtcgatatca tcgatcacct
     1261 cttctttgac aatggcatag gaggtcgctc cttctattcg ccggagtgtt tccggaacag
     1321 tgatgccttc ggccgccttt accatagcca aaagctcctg cctggatttt tctttgaggc
     1381 tcgcgatatc cgcctcggtc ggtactttga cgtcgcccac gaatccgccg gacatggctt
     1441 ccttggattc gccgtaaaat ccgttgaatt tcggcgtacc ctcgaatccg ggtatgcgcc
     1501 agctttgtcc ggccggaaga ttgtaatcgc tccccggttt ttccgccgtc actttgacgt
     1561 cgatcgacga agccactatt ttgccttcgc tgatcgccgc cccgggaata atgatcgcct
     1621 cgtttgtttt gaaaatcttg ccgccggtgg tgatgaaacg cgttcctttg acgatcgact
     1681 gcttgctcga actgtaagcg ttgtaaaccg tgatgacgcc ttcagcgctc tcttccactc
     1741 ttttggtgcc gctcgccgga gcgctgaaaa cattgttttt atcgatcgag aatatttggc
     1801 cgggaatggt gaatccggtt tcggcgctcg ccttgatcga agcggtgatg gcgatcggcg
     1861 tttcccagcg cgttttcacg agcgtcagac gcgcctccgc cttcggcaaa acgaaaacgg
     1921 cggcgtaagc caaaagtccg gcgaaaagga tccccccgga cgagaaaacg attattttaa
     1981 tatttcttga tttccgcggc cgatactcgg acgcgggctt gggcgacggt tcgggatcgt
     2041 attcgcggat aatcggttct gtttccggga ctttttcttc ttccggttga gtttttatcg
     2101 cgggttcttc ccgaataaag ggaacgcgcg ttggtcgcgg ctttactcga gcggagaccg
     2161 gaatgatgtc ggccacggcc cgtttcactt ttttgaaaac agggttgatc acctggaaac
     2221 ccgaagccaa agcgagatcg acgatgtctt cgtcgctcga ttcgataatc acctgcttgc
     2281 ccatggccga agcctcgcgg aaaatcagat aaaaattgtt tccgttttct ccgagcagag
     2341 aatatctcgg aacggccaaa ataaccttgg actcgcggct gttcaagatt ctctggatga
     2401 ccgcggccgc ggaatcggtt ttcttgaggt atattttttt gttttctggt ttttccatca
     2461 gtttatattg agccacttga tgcgctgctt ggcgaaacgg ttaaaattct tattcgaaga
     2521 aaactggacg aattcgcgct tcaggatgtc cccgggcttt tcccagggca aaaacgcgaa
     2581 cttggctccg gtgccgaaag taaagggctt ggccgaagct gctatctgat cgcacatgac
     2641 gaacgctatc ggctttacca ttttagcatt atgaatcgcc gctgacacgc ctttttcgaa
     2701 aacggaaaaa tgcttgttga aaacgaatct gaatttcttc agaaaatcgg gtgaagcgtc
     2761 gttttggaga taacggttca ataccggcgc caccgaatcc ttcggaaccg tcaagtcatc
     2821 gtggaacgat tggagaagat tatcgagacc ccactcgaaa tccccggccg gccggatgtc
     2881 tccgttttcc ttggctgcgc aaacgtgggt tttatccctc aagacgttaa cgaaaacaaa
     2941 gtttttgcag cggctttctt cccggagcaa atgggatttg accgaggtcg gttcgctcac
     3001 aaatattatt tctccgtttt tcggcaatcc ttgccggatt ttttggaacg tgtcgcgact
     3061 cgccaaagtt tccgccactc cgacctcgat tcttttggcc gggaaaccga tcgggttcag
     3121 aactttcgat ccgtccaccc tggccgagac gatgcgggcg tcgatcagaa ggacgtcgat
     3181 ctccggcacg ccgagcccga cccgtcccgc cgcggcaggg ccggcgc
//
LOCUS       DAOPQT010000062         3155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_33439_length_3155_cov_3.689355, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000062 DAOPQT010000000
VERSION     DAOPQT010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33439_length_3155_cov_3.689355"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PKG74_02040"
     CDS             complement(<1..1215)
                     /locus_tag="PKG74_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOB90093.1"
                     /translation="MLNIKKGDLSFKNAAGRILKAKIRGFTLMELLVYLGVFSIASVF
                     LTNILLTVTDITGRESASREVSSQLDATLETIQALIQQSSNIESASGSTLKLRMSAPA
                     SDPTCITLESGVIKIAQGPGTPADECSTTKTSLTTGSVVTNSLTFTKIDNIRYSEAEG
                     RELVGHSVVNIDMSMTYNSTNPKYAISRTLTSAIGRVSAATFDSDLLPDTTNTRSIGT
                     SALRWQNLNVNGTAYFAGNVGIGVSTPSQKLEINGNIKQGRMILWTGTIGNFALAANQ
                     VGYINFGDIAWNGQIEVELIGGWNHNTAFGALTRRFSFHHAVGGTSLSNYTTEVVDAS
                     GGLASNYAIGDAEIYETSKVRIPIYQINAAASNTIYAKVRIFATNPDNLVNVITVTAP
                     ATVENTATKNKVS"
     gene            complement(1272..1877)
                     /locus_tag="PKG74_02045"
     CDS             complement(1272..1877)
                     /locus_tag="PKG74_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOB90094.1"
                     /translation="MRKVLAHFKKNDPKIYFYLQTDPKIERLKPKDRKDYFSRLCVDI
                     IFQQISGKAGDAILAKFRSLFPKKRISAKKFLALGEPEIRAAGISAQKAKYLKSLAEH
                     FCGKKIRIKDFDKMTDEEVITELTRIKGIGRWTAEMFLMSTLGRENVFSAGDLGLRKG
                     LMKVYGFDEYPPEKKVEKITSAWAPYKTYGCLALWRSNDMP"
     gene            complement(1884..2465)
                     /gene="cyaB"
                     /locus_tag="PKG74_02050"
     CDS             complement(1884..2465)
                     /gene="cyaB"
                     /locus_tag="PKG74_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOB90095.1"
                     /translation="MACKNIEIEIQVNVEKVEPLLKFLEKNGVFQSEKRQLDEYFSPT
                     HRDFLSSRPVEEWLRLRDAEGKYSLNYKKWHYDDLGKSLYCDEFETKIENLETVKNIF
                     SVLNFKSLMVVDKLRKTWNYKDYEIAVDAVKNLGDFVEIEYIGEDEKIDPAKTIEEMV
                     GFLKKLGCGKIKRNFVGYPFALLFPDEVKYEIQ"
     gene            complement(2467..2961)
                     /locus_tag="PKG74_02055"
     CDS             complement(2467..2961)
                     /locus_tag="PKG74_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOB90096.1"
                     /translation="MENVKLRDATLVFLIKKSEDRVVDICLAMKKRGFGANRWNGVGG
                     KVITEETIEEAAKREAREEIGVSIHDLRKTAELSFHFPHNPAWDQLVHVYFSENWEGE
                     PKESEEMNPKWFSTSEIPFENMWPADIFWLPEALKGSLLRAMIQFGENDIVEDKEINI
                     IESF"
BASE COUNT          755 a          798 c          658 g          944 t
ORIGIN      
        1 agatactttg tttttggttg ctgtattttc aactgttgcc ggcgccgtta ccgtaatgac
       61 gtttaccaag ttatccggat tggtagcgaa tatcctgact ttggcgtaaa tagtgtttga
      121 agcggcggca ttaatttgat aaatcggaat ccggactttg gatgtctcat aaatttcggc
      181 gtctccgatg gcgtaattgc tggctagacc tcccgaagca tccaccactt cggtcgtata
      241 attgctcaaa gacgtaccgc cgacggcatg atggaaacta aaccttctgg taagagctcc
      301 gaaagccgtg ttgtggttcc aacctcctat taactccact tctatttgtc cgttccaggc
      361 gatgtctcca aaattgatat agccgacctg gtttgccgct agcgcgaagt tgccgatggt
      421 acccgtccac aaaatcatcc tgccttgttt gatgttgccg tttatttcca atttttgact
      481 cggcgtggaa acgccgatac cgacgttgcc cgcgaaataa gccgtaccgt tgacgttcaa
      541 attctgccat ctcaaggcgc ttgtgcctat gctccgggta ttggtcgtgt ccggcagcaa
      601 atccgaatca aaagtggcgg ccgaaacccg gccgatggcc gaagtcaaag tgcgggaaat
      661 cgcgtatttc ggattggtgg agttgtaggt catgctcata tcgatattga ccaccgaatg
      721 accaaccaat tccctaccct ctgcttccga ataacggata ttgtcgattt tggtgaaagt
      781 caaagaattc gtcaccacac tgccggtcgt caagctggtt ttcgtggtcg agcactcgtc
      841 ggccggcgtt cccggaccct gggctatttt gatcacgccc gattccaaag tgatgcaggt
      901 aggatcggac gccggagcgg acattctcaa tttcaaagtc gatccgctgg cggattcgat
      961 gttactcgat tgctggatca aagcctgaat cgtttcgagc gtcgcgtcga gctggctcga
     1021 aacttccctc gaggcggatt cgcggccggt gatatccgtc acggtcaaaa gaatattggt
     1081 gagaaaaacc gaggcgatcg aaaaaactcc caaataaacc aaaagctcca tcaaggtaaa
     1141 tccccgtatt ttcgctttta aaatccggcc cgccgcattt ttaaaagaca aatctccttt
     1201 ttttatattg agcattgttt taaaaatccc gacaattcta ttggattaat tataacataa
     1261 cgcttcccga atcagggcat gtcgttgctc cgccacagag ccagacatcc gtaagttttg
     1321 taaggcgccc aagcgctcgt gattttttcc acttttttct ccggcggata ttcgtcgaat
     1381 ccgtaaacct tcatcagtcc tttccgcaat cccaaatccc cggccgaaaa aacattctcc
     1441 cttcccaaag tggacatgag gaacatctcg gcagtccagc ggccgatgcc cttgatgcgg
     1501 gtaagttcgg tgatcacttc ttcatccgtc atcttgtcga aatcctttat tctgattttc
     1561 ttcccgcaaa aatgctcggc caggcttttc aggtattttg ctttttgcgc cgaaataccg
     1621 gctgcccgga tttccggttc tccgagagcc agaaattttt tggccgagat tctctttttg
     1681 gggaaaagcg atcggaactt ggcaagaatg gcgtctccgg cttttccgga aatctgctgg
     1741 aagatgatat ccacgcaaag acggctaaaa taatcttttc ggtctttcgg tttcagccgc
     1801 tcgatcttcg ggtcggtttg gagataaaag tatattttcg gatcgttttt cttgaaatgc
     1861 gcgagaactt ttcgcatgat cgactattgg atttcgtatt tcacctcgtc cggaaaaagc
     1921 aacgcgaaag gataaccgac aaaattcctt ttgatctttc cgcagccaag ttttttcaag
     1981 aaaccgacca tttcctcgat cgttttcgcc ggatcgattt tttcgtcttc accgatatat
     2041 tcgatttcca cgaaatctcc gagattttta actgcgtcga ccgcgatttc gtaatctttg
     2101 taattccaag tttttctcaa cttatcgacc accatcaacg atttgaaatt aaggaccgaa
     2161 aaaatattct ttaccgtttc cagattttca atcttggttt cgaattcgtc gcaataaaga
     2221 cttttaccaa gatcgtcata atgccatttt ttgtaattca aagagtattt tccttcagcg
     2281 tcccgcaatc tcaaccattc ctcgaccggc cggctggaaa gaaaatcccg gtgggtcgga
     2341 gaaaaatatt cgtccagctg gcgtttctcc gactggaaaa cgccgttctt ttccaaaaac
     2401 tttaaaagtg gctcgacttt ttcgacgttg acttgtattt ctatttcgat attcttacag
     2461 gccatatcag aaactctcga tgatatttat ttccttatct tccactatat cgttttctcc
     2521 aaactggatc atcgcccgaa ggagactacc tttaagcgct tccggcagcc agaaaatatc
     2581 ggctggccac atattctcaa aaggtatctc gcttgtggaa aaccatttcg gattcatctc
     2641 ttcgctttct tttggctcgc cttcccagtt ttcggaaaaa taaacatgga ccaactggtc
     2701 ccatgccgga ttatggggaa aatggaaaga cagttcggcg gttttccgga gatcgtgtat
     2761 ggaaaccccg atttcttctc tggcttccct tttggccgct tcttcgatcg tttcttccgt
     2821 aatcactttg ccgccgactc cgttccatct gttcgccccg aaaccccgtt ttttcatggc
     2881 aagacatata tcgacgactc tgtcttcgga cttcttgata aggaacacga gcgtagcgtc
     2941 ccttaatttg acattctcca tttgactatc ttaacagaaa tcaatctaat ccgtaattga
     3001 actcgtcttt ctattcgaga ccgtgatttc ggaattcttc ttttaatctc gatcgggtaa
     3061 atgtctttaa ccggcgacag acgcagattt ctactactcc tttccctgat tactaaccaa
     3121 gcttatctca caatcttata tatcttcccc gcatt
//
LOCUS       DAOPQT010000063         3140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_33639_length_3140_cov_3.872934, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000063 DAOPQT010000000
VERSION     DAOPQT010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33639_length_3140_cov_3.872934"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /gene="tuf"
                     /locus_tag="PKG74_02060"
     CDS             <1..214
                     /gene="tuf"
                     /locus_tag="PKG74_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413323.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOB90097.1"
                     /translation="PGYKPQFYMRTTDVTGEMILPQGMEMAMPGDTVNVTIKLLAPIA
                     LEEKQHFAIREGGKTVGAGVISKIVK"
     gene            229..534
                     /gene="rpsJ"
                     /locus_tag="PKG74_02065"
     CDS             229..534
                     /gene="rpsJ"
                     /locus_tag="PKG74_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOB90098.1"
                     /translation="MEKLRLRIKSYDQKLVDNSCKQIMEVLERMDTKIVGPMPLPTEF
                     KKYTVNRSTFVHKNAREQFEMRIHKRLIDILNPTQKLIESLSDLNLPAGVDIEIKTV"
     gene            613..1035
                     /gene="rplC"
                     /locus_tag="PKG74_02070"
     CDS             613..1035
                     /gene="rplC"
                     /locus_tag="PKG74_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOB90099.1"
                     /translation="MAYITAKKIKMSQIFKEDRVIPVTLVMVLDLPENMEIAENALVK
                     IVGTTKGRGFQGGVKRWGFHGGPKTHGQKDRLRAPGSIGATAPQRVLKGHKMAGHMGV
                     DRKTVKNVKVIAWNSEAKTMALKGAIPGMSGSKIKIYL"
     gene            1037..1675
                     /gene="rplD"
                     /locus_tag="PKG74_02075"
     CDS             1037..1675
                     /gene="rplD"
                     /locus_tag="PKG74_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOB90100.1"
                     /translation="MKADLYNAKMEVIDQVEIPDRIFGYPWKPDLVKQALDSQEKNSR
                     QPFAHAKGRGEVSGGGKKPWRQKGTGRARHGSTRSPIWKGGGVAHGPKKERDYSVKLN
                     QKMRQAAIMSVLSKKLEDNEVKFVDSLAVSEPKTKVLHSTLRNFFGKKAPNALLIPEI
                     GNKNVHRASSNLPKVKSLGPKDLNVYDLMKFKEIFIEKNAISAIDSHYHAVK"
     gene            1662..1934
                     /gene="rplW"
                     /locus_tag="PKG74_02080"
     CDS             1662..1934
                     /gene="rplW"
                     /locus_tag="PKG74_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOB90101.1"
                     /translation="MPSNKEKKTKDNKEKTDIRFYLSEKAGRLGGFNQFVFIIKNTLN
                     KSEVRKQIEKKYKVNVVAMNITRISKKNLKKAIVTLKKGQTINEKV"
     gene            1921..2760
                     /gene="rplB"
                     /locus_tag="PKG74_02085"
     CDS             1921..2760
                     /gene="rplB"
                     /locus_tag="PKG74_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOB90102.1"
                     /translation="MKKYNPTTPSRRHMTAPDYRSVLSGVKPEKKLTMNLQERAGRNN
                     RGRITMRHQGAGNKRTYRIIDFRRNDKLNIPARIESLEYDPYRTAFIMKLIYKDGTRR
                     YSIAPQGAKAGSTIVTAENAEFVPGNRLMLKNIPVGYSVFNIELNPGKGGQIVRSAGS
                     SAEVLGHDAGYTQLKMPSGEIRKVLWSNFATLGQVSNADWGLVNIGKAGRTRGMGVRP
                     TVRGTVMNPVDHPYGGGEGRQPRGTKKPKTKWGKITGGRKTRNQKKWSNVLITKRRLK
                     KKK"
     gene            2765..3088
                     /gene="rpsS"
                     /locus_tag="PKG74_02090"
     CDS             2765..3088
                     /gene="rpsS"
                     /locus_tag="PKG74_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOB90103.1"
                     /translation="MSRSSHKGPYVEASLLKKIAGLKPGDKTVVKTWARSSSIAPEMV
                     GFVFGVHNGKDFVEVRVTEEMVGHKFGEFAMTRKFVRHGGKMQKELEKGAGAPVAPAP
                     DKTAK"
BASE COUNT          980 a          748 c          832 g          580 t
ORIGIN      
        1 ccccggatac aagccgcagt tctacatgag gaccacggac gtcaccggtg aaatgatcct
       61 gccgcaagga atggaaatgg ccatgcccgg agacacggtc aacgtgacga tcaagcttct
      121 cgctccgatc gctctcgaag aaaaacagca cttcgccatt cgcgaaggag gaaagaccgt
      181 tggcgccgga gtcatctcca aaatcgtcaa ataattcgtt actctaacgt ggaaaagctc
      241 agattaagaa tcaaatcgta cgaccagaag ctggtggaca attcctgcaa gcagatcatg
      301 gaggttctgg agcggatgga taccaaaatc gtcggtccga tgccgcttcc gaccgagttc
      361 aaaaaataca cggtcaaccg ctcgactttc gtgcataaga acgccaggga gcagttcgag
      421 atgagaatcc acaagaggtt gatcgacatt cttaatccga cgcagaaact gatcgaatcc
      481 ttatccgact tgaatctgcc cgcgggggtg gacatagaaa taaaaacagt gtagtattgg
      541 atagaggata gccaacaaga aatcccacaa aataaccgag gcttcctcgg ttattttgtg
      601 ggcgtcaaac aaatggccta cattaccgcg aaaaaaatca aaatgagcca gatcttcaag
      661 gaagacaggg tgattccggt tactttggtt atggttttgg atctgccgga aaatatggaa
      721 atcgcggaga acgctttagt caaaatcgtc ggcaccacca agggccgcgg gttccaggga
      781 ggagtcaaac gctggggttt tcacggcggc ccgaaaacgc acggacaaaa agaccggctt
      841 cgcgctccgg gatctatcgg cgccaccgct ccgcaaagag ttctgaaagg ccacaaaatg
      901 gccggacaca tgggtgtcga cagaaaaacc gtgaagaacg tcaaagtgat cgcctggaat
      961 agcgaagcga aaaccatggc tttgaaagga gctattccgg gaatgagcgg aagcaaaatc
     1021 aaaatctatc tctaaaatga aagccgatct ctacaacgcc aaaatggaag tgatcgacca
     1081 ggtggaaata ccggatcgga ttttcggata cccgtggaaa ccggatctgg tgaaacaggc
     1141 tttggacagc caggagaaaa acagccgtca gccttttgcc cacgccaaag gccgcggcga
     1201 agtttccgga ggcggcaaaa agccgtggcg ccaaaaagga accggccgcg ccaggcacgg
     1261 atcgaccagg tcgccgatct ggaaaggcgg aggcgttgcc cacggcccga aaaaagaaag
     1321 ggattattcg gtcaaactca accagaaaat gaggcaggcg gccatcatga gcgttctttc
     1381 caaaaaactg gaagacaacg aagtaaaatt cgtcgattcc ttggccgtca gcgaaccgaa
     1441 aaccaaggtt cttcattcca cgcttcggaa tttcttcggt aaaaaagcgc cgaacgcgct
     1501 tttgataccg gagatcggaa acaaaaacgt tcatcgggcc tcatccaatc tgccgaaagt
     1561 gaagagcctc gggccgaagg acttgaacgt ctacgatctc atgaaattca aggagatttt
     1621 catagaaaag aacgcgattt ccgcgataga ttcccattac catgccgtca aataaagaaa
     1681 agaaaaccaa agacaacaaa gagaaaaccg atatccggtt ttatctcagc gaaaaagccg
     1741 gaagactcgg cggcttcaat cagtttgttt ttataatcaa gaacaccttg aacaaaagcg
     1801 aagtcaggaa acagatcgaa aaaaaataca aggtgaacgt cgtcgccatg aacatcaccc
     1861 ggatttccaa aaagaatctg aaaaaagcca tcgtcacttt aaagaaagga caaaccatca
     1921 atgaaaaagt ataatccgac aactccatcg agaagacaca tgacggcgcc cgactaccgt
     1981 tcggttttga gcggggtgaa gccggaaaag aaattgacga tgaatctcca ggagagagcc
     2041 ggccggaaca accggggccg catcaccatg aggcatcagg gagccggcaa caagcggacc
     2101 taccgcatca tcgatttccg caggaacgac aaattgaata tcccggcgag aatcgaatct
     2161 ttggaatacg atccttaccg tacggctttc atcatgaagc ttatctacaa agatggaacc
     2221 aggcgttatt cgatcgcgcc ccagggagcg aaagcgggct cgacgatcgt taccgcggaa
     2281 aacgcggagt ttgttccggg aaacagattg atgctcaaga acattccggt cggatactcc
     2341 gtttttaaca tcgaattgaa tccgggcaaa ggcggacaga ttgttcgttc cgccggttcg
     2401 tcggccgaag ttttgggaca cgatgccgga tacacccagc ttaagatgcc ttccggagaa
     2461 atccgcaagg ttctttggag caatttcgcc actttgggtc aggtttccaa tgccgattgg
     2521 ggtctcgtta atatcggaaa agccggtagg actcggggca tgggcgtgag acccacggtt
     2581 aggggaacgg tcatgaatcc ggttgatcat ccttacggcg gaggcgaagg cagacagcct
     2641 cggggcacca agaagccgaa aaccaaatgg ggtaagatca ccggaggcag aaaaaccaga
     2701 aatcagaaaa aatggtcgaa cgttttgata accaaacgca ggctgaagaa aaagaaataa
     2761 aaccatgtcg agaagcagcc acaagggtcc atacgtcgaa gcgagtcttt tgaaaaagat
     2821 cgcgggtttg aaaccgggag acaaaacggt tgtcaaaaca tgggcgcgct cgagctcgat
     2881 cgcgccggag atggtcggat ttgttttcgg agtccacaac ggaaaagatt ttgtcgaagt
     2941 gagggtgacc gaagaaatgg tcgggcacaa gttcggagaa ttcgccatga cccgcaagtt
     3001 cgtccgccac ggcggaaaga tgcaaaaaga attggaaaag ggcgcgggag ctccggtcgc
     3061 tccggctccg gataaaaccg ccaaataagc accatgcaag agataaaggc aaaattgaaa
     3121 aatctggacg tggctccgcg
//
LOCUS       DAOPQT010000064         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_33899_length_3123_cov_3.818123, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000064 DAOPQT010000000
VERSION     DAOPQT010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33899_length_3123_cov_3.818123"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..557
                     /gene="avd"
                     /locus_tag="PKG74_02095"
     CDS             216..557
                     /gene="avd"
                     /locus_tag="PKG74_02095"
                     /inference="COORDINATES: protein motif:HMM:NF033474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diversity-generating retroelement protein Avd"
                     /protein_id="HOB90104.1"
                     /translation="MIDDLIVYQKTYDFMLWLHPVVNKFPKSQRFVLGQRIENKTLDL
                     IHSMLSANIEREKSVLLKQASVELDELRMLIRLAKDLHFVSVKQYGVAAEKMNEIGKL
                     LFSWMKKFSPK"
     gene            938..1936
                     /locus_tag="PKG74_02100"
     CDS             938..1936
                     /locus_tag="PKG74_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HOB90105.1"
                     /translation="MTLFEQITDFENLHRAYLGARQCKRYRFSILKFGRRLEENLLSL
                     QKELQGKTYKHGKYREFVVSDSKKRTIRAAPFRDRVVHHAVCRAIEPIFDKGFIYDSY
                     ACRKGKGTHAAVRRLESFLRSLKTKAKGRRLYCLKCDVSKYFDNVDHELITKMIRRKI
                     PDKDLLWLVGEILESHKQGIPIGNLTSQLFANIYLNELDYFVKRTLREKYYLRYMDDF
                     LILGLDKKRLHESKELIRKFLREQLKLELHPKKAEVFPADKGVDFLGYVIRGNKRFLR
                     KSTVKRFLKKKHRLEAMVRNNVLSEQSWENSRASWRGYAKFADSHKLLEKLGQSLW"
     gene            complement(1938..2615)
                     /locus_tag="PKG74_02105"
     CDS             complement(1938..2615)
                     /locus_tag="PKG74_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOB90106.1"
                     /translation="MKGIILAAGKGVRAKPLTLETPKPLIKVKGKTLLERLTEKLPSE
                     IDELLIVTGYLAEQIESFVGNEFLGRKTICVRQPEQRGTFDALCRCEPLIDNERFAVF
                     FPDDLLGHQAIAECLKHDLAAAVKETTDPEKFGVVLVDENGFIADAVEKPKDPFSDLA
                     LASGYVLDPDIFRFPPRLGANDEYHLSEAIAGLSKSRKVKAVKSDFWFPVSSAEDILR
                     AEEVLEE"
     gene            complement(2676..2747)
                     /locus_tag="PKG74_02110"
     tRNA            complement(2676..2747)
                     /locus_tag="PKG74_02110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2713..2715),aa:Val,seq:tac)
     gene            complement(2786..3112)
                     /locus_tag="PKG74_02115"
     CDS             complement(2786..3112)
                     /locus_tag="PKG74_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90107.1"
                     /translation="MFTGAPDSITKLPPPPGGFWVGFCQAKATDFSMAKITKVVIRVF
                     WRKVNPLTPPPALQGVFLMIGEDIHGLVIFVIILVGFIIASSIFWFPIKVSRETFVFT
                     QICFTP"
BASE COUNT          848 a          772 c          766 g          737 t
ORIGIN      
        1 tggggcacgg gcgatgacaa ggcgactacc gtcttcctac gcggcggcga ttggagccct
       61 tcgtcgcacg cgggagcatt tgctctgaac ttgctttgga cggcgtccta cacgggcagc
      121 aatgtggggt ttcggtgcgc tcggtagcct aacgtagaaa tcttgaatct gaaatctttc
      181 gtcaaaaaaa ttaaaccgaa agaaaatatt tgactatgat cgatgacctc atcgtctatc
      241 aaaaaacata cgacttcatg ctctggctcc atccggtcgt caacaagttt cccaaaagcc
      301 agagattcgt tctggggcag aggatagaga acaaaaccct cgaccttatc cactcgatgc
      361 tttcggcgaa catcgagcgc gagaaatccg ttttgctgaa acaggcgagc gtggagctcg
      421 acgaattgag aatgcttatc cggctcgcca aggatttgca tttcgtgagc gtcaagcagt
      481 acggcgtggc cgcggagaaa atgaacgaaa taggcaaact ccttttctcc tggatgaaaa
      541 agttttcccc gaaataaact ataatataat tgtccgcccc ggaatcgatt ttccgcgcgg
      601 atagggataa aggcgtaaat taacggcgtc ttcctacgcg gcggcaattg gaacaattcg
      661 tcgaacgcgg gagcatttgc tctgaacttg aattagacgg cgtccaacac gaacaacaat
      721 gtggggtttc ggtgcgctcg gtacccatcg ataaggatcg tttgacgatc cttggcccga
      781 gcccggagtc gaaagattcc gggactgcgc caagatggct actgctttta ttccttcttc
      841 agtagggctt agcgtccgaa agggaaaata tgacctgttt ccccgcgcca ttgttttttg
      901 acccactggc gcggggtaat agggttctcg accggacatg actctttttg aacagatcac
      961 cgattttgaa aatctgcacc gcgcctatct cggcgcccgt cagtgcaaac gctatcgttt
     1021 ttccattttg aagttcggcc ggcggctgga agagaatctt cttagcctcc aaaaggaatt
     1081 gcaaggaaag acctataaac acggaaaata ccgcgaattt gtcgtaagcg actccaaaaa
     1141 gcggaccatc cgggcggcgc ctttcaggga ccgcgtggtt catcacgcgg tttgccgcgc
     1201 tatcgagccg attttcgaca aggggtttat ttacgattcc tacgcctgcc gcaaaggaaa
     1261 aggaacccac gcggccgtga gaagactgga aagttttttg cggagcctga aaaccaaggc
     1321 caaaggccgg cgcctctatt gcctgaaatg cgacgtttcc aaatatttcg acaacgtcga
     1381 ccacgaactg ataacaaaaa tgatccgccg gaaaatcccg gacaaagacc ttctttggct
     1441 cgtcggagaa atactcgaaa gccacaaaca aggcattccg atcggcaatc tgacgagcca
     1501 gcttttcgcc aacatctact tgaacgaact cgattatttc gtcaaaagaa ctttgcggga
     1561 aaagtattat ctccggtata tggacgattt cctgatcctt ggtttggaca aaaaacgcct
     1621 ccacgagtca aaggagctta tccggaaatt tctccgcgag caattgaagc tcgaattgca
     1681 cccgaaaaag gccgaagttt ttccggcgga caaaggcgtc gatttcctcg gatatgtcat
     1741 tcgcggaaac aaaagatttt tgagaaaatc cacggtcaaa agattcctga aaaagaagca
     1801 tcggttggaa gcgatggtga gaaacaatgt tctgtccgaa caatcctggg aaaattcccg
     1861 ggcttcgtgg cgcggctacg ccaagttcgc cgacagccac aagctcttgg aaaaattggg
     1921 gcagagtctt tggtaattta ttcttccaga acttcttcgg cccggagaat atcttcggcc
     1981 gaggagaccg gaaaccaaaa atcagatttc acggctttta ctttccgcga tttggacaaa
     2041 ccggcgatgg cttcggaaag atggtattcg tcgttcgctc cgagccgcgg cggaaatcgg
     2101 aagatgtcgg gatcgagaac atagccgctc gcgagcgcca gatcggaaaa aggatctttc
     2161 ggtttttcga cggcatccgc gataaagccg ttttcgtcga cgaggacaac cccgaacttt
     2221 tccggatccg tcgtttcttt gaccgcggcg gccaaatcat gcttcaggca ttcggcgatc
     2281 gcctgatggc cgagcaagtc gtccgggaaa aacacggcga atctttcgtt gtcgataagc
     2341 ggttcacagc gacaaagcgc gtcgaaagtt cctctctgtt cgggctgacg aacgcagatc
     2401 gttttccgtc cgaggaattc gttcccgaca aaactttcta tttgttcggc caagtagccg
     2461 gtgacgatca aaagttcgtc gatttccgaa ggcagctttt ccgtcaaacg ttccaaaaga
     2521 gtcttgcctt tgactttgat caaaggtttg ggcgtttcca aagtgagcgg cttggcgcgc
     2581 acgcctttcc cggctgccaa gattattcct ttcatttcaa taattataag ttattaatct
     2641 cgaacggaga ataatttggc cgcgccggga agatgtgcgc gctcaaggat tcgaacctag
     2701 gaccttctcg gtgtaaacga gacgctctgc cgctgagcta agcgcgctca aatcacgttt
     2761 attacaaacg aaaaaccttt taaaatcaag gtgtgaaaca aatttgtgta aacacgaagg
     2821 tttcacgtga aactttaatt ggaaaccaga aaatggaaga tgctataata aaaccaacca
     2881 aaataatcac aaaaatcacc aaaccatgaa tatcttcacc aatcatcaga aaaacacctt
     2941 gaagcgccgg ggggggggtt aaaggattaa cttttctcca aaaaacccta atcaccacct
     3001 tagttatctt tgccatcgag aaatcggtgg cttttgcttg gcaaaatccc acccaaaatc
     3061 ctcccggagg cggaggaagc tttgtaatcg agtccggtgc tcccgtaaac acgatttaca
     3121 tca
//
LOCUS       DAOPQT010000065         3122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_33916_length_3122_cov_2.762634, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000065 DAOPQT010000000
VERSION     DAOPQT010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33916_length_3122_cov_2.762634"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..1918
                     /locus_tag="PKG74_02120"
     CDS             20..1918
                     /locus_tag="PKG74_02120"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOB90108.1"
                     /translation="MKFSYYLLKKMVPGIPDKKKLADVLSVKSFEVEEVKADAMEIKI
                     MPNRWSDAASHWGMAREAAAATGRKLSFAPKIIVNQPQNKGFLEQIKIEDPKLCPRYM
                     AYYLELPKIGQSPVWMRKTLQTCGLRPVNAVVDILNYVMIETGQPLHAFDASKIKGGI
                     RVRRAKTNETIETIDNQKIKLDKSILVIADREKLLAIAGIKGGESSKVDSKTKAIIIE
                     SANFDPVSIYRASKTIKLATDASQRFGHGLSPELCKIGIDRAIVLLKEICKATFLDSK
                     DVYPKRQPKKILKFDEEKLQSVLGADIDSKTVSIILKSLGFRLLPKNMIEVPALRLDI
                     NIIEDLIEEVGRMYDLNKIPGKNPAVLLAPPEMDEYFLAAEKAKNSLMEMGFSEVRNS
                     SFVPAKTDGDVEMENPLSDDKRFLRNSLVYHLRENVKTNHRFSGNVRIFEIGKVFLKG
                     FKEEYHLAMAVSLKSGDYVFRELRGPAENLLRSLGLTDYAFVPEGSKLRLESDHEVLG
                     YLFPDPKNKAAFLEIDFDKTRHLMEGEREYLEISPYPSIIRDISFTVPASVRMNLILE
                     AIADLRIENLDDVDLIDYLNIADDRISLTLRLVFQSMKKTLSDEEANRSTAKIADILA
                     SDFKAEIR"
     gene            2000..>3122
                     /locus_tag="PKG74_02125"
     CDS             2000..>3122
                     /locus_tag="PKG74_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOB90109.1"
                     /translation="MAKKSEEKSVKKEQSYSAKDIEVLEGLEPVRKRPGMYIGSTGVD
                     GLHHLVWEIVDNSVDEAMGGYANNIRVELNKDGSVTVQDDGRGIPVDVHAVTKKSALE
                     TVMTTLHAGGKFGGAGYKVSGGLHGVGASVVNALSEHMKVEVRQNGFLYSQEYERGKP
                     KHAVKKIGKSVGRGTTSIFYPDKQVFSETIFDRKRILDHLREQAYLTKGLRIVFIDRR
                     EETPFYYAFRFAGGLRSFIDYLNEDGDPLIQQEVFYVNKEYNGMDIEAALAYTEGFEV
                     EEYSFANNIHTSDGGMHLTGLRTAITRTLNDFSRSSGYLKETDDNLTGEDVREGLIAV
                     VSVKLKNPQFEGQTKARLGNPEARSAVDTVVSEAFKEFLE"
BASE COUNT          851 a          755 c          800 g          716 t
ORIGIN      
        1 catcaaacaa ttctagatca tgaagttttc ctattatttg ctcaaaaaaa tggttccggg
       61 gattccggac aaaaagaaat tggccgatgt tttgagcgtt aaatcctttg aggtagaaga
      121 ggttaaagcc gacgccatgg aaatcaagat catgccgaac cgctggtcgg acgccgcttc
      181 ccattgggga atggcgaggg aagccgcggc cgcgaccggc cgaaagcttt ctttcgctcc
      241 aaagatcatc gtcaatcaac cgcagaacaa aggctttttg gaacaaatca agatcgaaga
      301 tccgaagtta tgtccgagat atatggctta ttatctcgag cttccgaaaa tcggacagtc
      361 tccggtttgg atgcggaaaa cgcttcaaac ctgcggtttg cggccggtga acgctgttgt
      421 cgacattttg aattacgtta tgatcgaaac cggccagccg ctccatgctt tcgacgcctc
      481 gaaaatcaaa ggcgggatcc gagtccgccg agccaaaacg aacgaaacaa tcgagacgat
      541 cgacaaccag aagatcaaac tcgacaagtc gattttggtc atcgccgacc gcgaaaaact
      601 tttggccatc gccggtatca aaggagggga aagctcgaaa gtggattcca aaaccaaagc
      661 gatcatcatc gagtccgcca atttcgaccc ggtttcgatt tacagggctt ccaaaacgat
      721 caaactcgcg accgacgctt cacaaagatt cggtcacggc ttgtctccgg agctttgcaa
      781 gatcggcatc gaccgggcaa tcgtgctgct caaggaaatc tgcaaagcga cttttctcga
      841 ttccaaggat gtttatccga aacgccaacc gaaaaagatt ttgaaatttg atgaggaaaa
      901 gcttcaaagc gttctcggag cggatatcga ttccaagact gtttcgatta ttttgaaatc
      961 tttgggattc cgtctccttc cgaaaaatat gatcgaggtt ccggctttga ggctcgacat
     1021 aaatatcatc gaggacttga tcgaggaagt cggtcggatg tacgacctga acaagattcc
     1081 cgggaaaaat ccggctgttt tactcgcgcc gccggaaatg gacgagtatt tcttggccgc
     1141 ggaaaaagcc aagaatagtc ttatggaaat gggattttcc gaagtccgca attccagctt
     1201 tgttccggct aaaacggatg gagacgtcga aatggaaaat cctctgtccg acgacaagcg
     1261 attcctccgg aacagccttg tttaccatct tcgggagaac gtcaaaacca atcatcggtt
     1321 ttccggaaac gtgagaatat tcgaaatcgg caaagttttc ctcaagggct tcaaggaaga
     1381 atatcatttg gccatggccg tcagtctgaa atccggcgac tatgttttcc gcgaacttcg
     1441 cggaccggcc gagaatctgc tgcggagtct cggacttacg gattacgctt ttgtgccgga
     1501 aggaagcaaa ctgcggctgg aatccgatca tgaagttttg gggtatcttt tccccgatcc
     1561 gaaaaacaaa gccgcttttc tcgaaatcga tttcgacaaa acgcgtcatc tgatggaagg
     1621 ggaaagggaa tatctcgaga tctcgccgta tccttcgatc atccgcgaca tttcctttac
     1681 ggttccggct tccgtgagaa tgaatcttat tctcgaagcg atcgccgatt tgaggatcga
     1741 gaatctcgac gacgtcgatt tgatcgatta tctgaatatc gccgacgacc ggatcagcct
     1801 cactttgcgc ctggttttcc aatccatgaa aaagacgctt tcggacgagg aagccaaccg
     1861 ttcgacagcc aaaatcgccg atattttggc ctctgatttc aaggccgaaa tccgctaaga
     1921 cgagaggcct gaagggttgt ctaaagccct gatttttgtt agaataaaaa gactttttga
     1981 agttttaaac atctggaaaa tggccaaaaa aagcgaggaa aaatccgtta aaaaagagca
     2041 gtcgtacagc gccaaagaca tcgaggttct ggagggtttg gaaccggtca gaaaaaggcc
     2101 tggaatgtac atcggttcca ccggcgtcga cggcttgcat cacttggttt gggagatcgt
     2161 cgacaactcg gtcgacgaag ccatgggcgg atacgccaat aatatccgcg tggaattgaa
     2221 taaagacggc agcgtaacgg ttcaggacga cggccgcggc attccggtcg atgtccacgc
     2281 cgtgaccaaa aagtccgcgc tcgagaccgt gatgaccacg ctccacgccg gcggcaaatt
     2341 cggcggagcc ggatacaagg tttccggtgg tctgcacgga gtcggagctt cggtcgtaaa
     2401 cgctttatcc gaacacatga aagtcgaagt ccggcagaac ggttttcttt acagtcagga
     2461 atacgagcga ggcaagccca agcacgccgt gaaaaaaatc ggcaaatccg tcggtcgcgg
     2521 caccacttct attttttatc cggacaagca ggtgttttcc gaaacgattt tcgaccggaa
     2581 aagaatctta gaccatctgc gggagcaggc ttatctcacc aaaggcttga ggatcgtttt
     2641 tatcgaccgt cgggaagaga ctccgtttta ttacgccttc cggttcgccg gcggactcag
     2701 atcgttcatc gattatctta acgaagacgg tgatccgctc atccagcagg aagtctttta
     2761 cgtcaacaag gaatataacg gcatggatat cgaagcggct ttggcttaca ccgaaggttt
     2821 cgaagtcgaa gaatacagct ttgctaacaa tatccatact tcggacggcg gcatgcattt
     2881 gaccggactc cggacagcca tcacgaggac tttgaacgat ttcagccgtt cgtccggata
     2941 tttgaaagaa acggacgaca acttgaccgg agaagacgtg cgcgagggac tgatcgccgt
     3001 tgtctcggtc aagctcaaaa acccgcagtt cgaaggccag accaaggcca ggttgggcaa
     3061 tcccgaggcg agaagcgccg tggatacggt cgtttccgaa gccttcaagg agtttttgga
     3121 aa
//
LOCUS       DAOPQT010000066         3094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_34325_length_3094_cov_3.097400, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000066 DAOPQT010000000
VERSION     DAOPQT010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34325_length_3094_cov_3.097400"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PKG74_02130"
     CDS             complement(<1..914)
                     /locus_tag="PKG74_02130"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90110.1"
                     /translation="MKKGFHEINFDEVTLDTESSRGFSVTEKDLDRRIFLGLGLVAAA
                     IGGLVFFRTGFLSIVKNKAYAERADANINKVIIESAPRGIIFDRYGKPIVENTPILSV
                     YLRPAELVKQNEMSPVLEALAKLGVDQNKLFSILNQSNLQETDTVLIKKDISNSEAID
                     LKAMGLNSLFVADDFKREFDESFAHVVGYTGLVTADDIKKRSLTGSDTVGKTGLEAAF
                     DESLRGVNGRTIVRRNVKGESLEEKSLTEPKIGNDLKTTIDAELQEYFYWRLEKGIND
                     LDLREGGAGIAFDPKTGEILSLISYPSFT"
     gene            complement(916..1182)
                     /locus_tag="PKG74_02135"
     CDS             complement(916..1182)
                     /locus_tag="PKG74_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90111.1"
                     /translation="MISFGNLLLGFVVATSFFFRNWWQRILIFSIAGFFVKFYPGFDM
                     TLLFFILSAAFGSWLGDFLRKKISFGFVIAILASEVVFNILISI"
     gene            complement(1175..1834)
                     /gene="mreC"
                     /locus_tag="PKG74_02140"
     CDS             complement(1175..1834)
                     /gene="mreC"
                     /locus_tag="PKG74_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOB90112.1"
                     /translation="MRKNNRLLLFTALIIGTYLVCGGENVLSWRHQLLSFAMESGSEI
                     DNLKIKNLELEAQLLNLKIGNKETEGQKVAAKVYSLYPFANRSEIVINKGSGSGVEEG
                     MAVVVENKIMVGRVKRVYPNSAVVQTIFDKDFKTPVRIGESETDAFYDGGLTAKLSLI
                     DAKSPAKEEDMVFTADKNLPYGLVLGKAVNITSGSPLKEARLQPTYELKNLRNVVVIV
                     D"
     gene            complement(1850..2893)
                     /locus_tag="PKG74_02145"
     CDS             complement(1850..2893)
                     /locus_tag="PKG74_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOB90113.1"
                     /translation="MNIFGKFSKDVGIDLGTTNTLIYLKNKGLLVNEPTIVAVNNKTG
                     QIVAIGNEAKKMLDRTPQHIDVIKPLTGGVISDFEMTEEVIKYHLRNLSEGIFNRYGL
                     AVLGVPTHLTEVERKSVEDAALSAGVSKVYLIEEPIAAALGARLPVEDPTANMIVDVG
                     GGTTEIAIISIGGTVISNSLKTAGDKLNEDIIRFVRDEFKLAIGEPTAEDLKISIGSA
                     IPLDEKLEMTVHGRDLSTGLPKEAVIKNSHVRMAISRSLKTIAEASKSLIEEAPPELV
                     GDILERGVYICGGGSMLRGFDQYLSKEIASQATLIDDPLTCVIRGVGMIIEDFGHYEK
                     ILDSQAKPREIKI"
     gene            complement(2871..>3094)
                     /locus_tag="PKG74_02150"
     CDS             complement(2871..>3094)
                     /locus_tag="PKG74_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOB90114.1"
                     /translation="GSAAKIYKRMLEDGLEVLYDDRDVSPGEKLADADLIGIPYRLIV
                     SEKTGKKIELKKRTDKKTTLVNYEYLRKI"
BASE COUNT          696 a          810 c          760 g          828 t
ORIGIN      
        1 gtgaagctcg gatagctgat gaggctcaag atctcgccgg ttttcggatc aaaggcgatg
       61 ccggcgcctc cttcccgaag atccaaatca ttgattcctt tttcgagccg ccagtaaaaa
      121 tattcctgaa gctcggcgtc gatcgtcgtt ttgaggtcgt ttccgatttt cggctcggtc
      181 aaagattttt cttccaaaga ttctcctttg acgttacgcc tgacgatcgt tcggccgttc
      241 actccccgca ggctttcgtc gaaggccgct tcgagtccgg tctttccgac ggtgtcgctt
      301 cccgtaaggc tgcgcttctt gatgtcgtcg gccgtcacga ggccggtgta gcccaccaca
      361 tgggcaaaag actcgtcgaa ttcccttttg aaatcgtcgg ccacgaaaag cgaattcaag
      421 cccatagctt tcagatctat ggcctcagaa ttggaaatgt cttttttgat caaaaccgta
      481 tccgtctcct gaagattgct ctgattcaat atggaaaaaa gcttgttttg gtcgacgccg
      541 agcttggcca aggcttcaag caccggagac atttcgtttt gcttgacgag ctcggccggc
      601 cgcagataaa cgctaagtat cggtgtgttt tccacgatcg gttttccgta gcggtcgaag
      661 atgatccccc ttggcgccga ttcgataatc actttgttga tgttcgcatc cgcgcgttcg
      721 gcgtaggcct tgtttttgac gatactcaaa aatccggtcc gaaagaaaac cagacctccg
      781 atcgcggccg ccaccagacc aagtcccagg aatatccgcc tatccaaatc tttttcggtc
      841 acggaaaaac ccctcgagga ttcggtgtcc agggtgactt cgtcaaaatt gatttcgtga
      901 aatccttttt tcatttcaaa tggaaatcaa aatattaaaa accacttccg aagcgagaat
      961 ggcgataaca aagccgaagc ttatcttctt ccgcagaaaa tccccgagcc agcttccgaa
     1021 ggcggccgac agaatgaaaa atagcagagt catgtcgaat cccggataga atttcacgaa
     1081 aaacccggcg atcgagaata tcagaatcct ctgccaccag ttcctgaaaa aaaagctcgt
     1141 ggcgacgaca aagccgagga gaaggttgcc gaagctaatc aacgatgacc acgacattgc
     1201 ggaggttttt gagttcgtag gtcggttgaa gccgggcttc tttgagtgga gagccggaag
     1261 taatattcac ggctttgcct aaaacaaggc cgtagggaag attcttgtcc gccgtgaaaa
     1321 ccatgtcttc ttccttggcc gggcttttgg catcgatcag gctcaatttg gcggtcagtc
     1381 cgccatcgta aaaagcgtcg gtctccgatt cgccgatccg gaccggggtt ttgaaatcct
     1441 tgtcgaaaat ggtctggacc acggccgaat tcggataaac ccgtttcacc cggccgacca
     1501 ttattttatt ctcaacgacc acagccatac cttcttccac cccagaaccc gaacctttat
     1561 tgatgacgat ttccgaacga ttggcaaaag gatagagtga atagaccttg gccgccactt
     1621 tttggccttc ggtttctttg tttccgattt tcagattcaa aagctgggct tcgagttcca
     1681 aattcttgat tttcaagttg tcgatttcac tcccggattc catggcgaaa gacaagagtt
     1741 ggtggcgcca agaaagaacg ttctctccgc cgcacacaag atacgttccg ataattaatg
     1801 ccgtaaataa aagcagccga ttgttcttcc gcatgactct tatattgtac tatattttga
     1861 tctcgcgagg cttggcctgg ctgtcgagaa tcttttcgta gtggccgaag tcttcgatga
     1921 tcatgccgac tcccctgatg acgcaagtca aaggatcgtc gatcaaagtg gcctggctcg
     1981 cgatttcctt cgaaagatac tggtcgaatc cgcgcagcat gcttccgccg ccgcagatat
     2041 aaactcccct ctcgaggata tcgccgacga gttccggcgg cgcttcctcg atcaggcttt
     2101 tcgaggcctc ggcaatggtt ttcaaagatc tcgaaatggc cattctcacg tgcgaatttt
     2161 tgatgaccgc ctctttcgga aggccggtcg aaagatcccg tccatggacg gtcatttcga
     2221 gtttttcgtc gagcggaatg gccgaaccga tggagatctt caagtcttcg gccgtgggtt
     2281 cgccgatggc caacttgaat tcgtctctga cgaatctaat gatgtcttcg ttcagtttgt
     2341 ctccggcggt tttcaggctg ttcgaaatca ccgtgccgcc gatcgaaatg atggcgattt
     2401 ccgtggtgcc gccgccgacg tcgacgatca tgttggcggt cggatcctcc actggcagac
     2461 gcgcgcccaa agcagcggcg atcggttcct cgatcagata aactttggag acgccggcgg
     2521 aaagagccgc gtcttccacg gatttccttt cgacttcggt caaatgggtc ggcacgccaa
     2581 gcacggccaa tccgtagcga ttgaaaatgc cttcgctcaa gttccgaaga tgatatttga
     2641 tgacctcttc ggtcatctcg aaatcggaga tgactcctcc ggtcaaaggt ttgatgacat
     2701 caatgtgctg gggcgtccgg tcgagcattt tcttggcttc gttgccgata gccacgatct
     2761 ggccggtttt attgttgacc gccacgatgg tcggttcgtt caccaaaagc cccttgtttt
     2821 tgagatagat taaagtgttg gtcgttccga ggtcgatgcc gacatctttg ctaaattttc
     2881 cgaagatatt catagttcac cagtgttgtt ttcttatcag tccttttctt caattctatt
     2941 ttcttgccgg ttttctcgct cacgatgagc cgatacggaa tgcctatcaa gtcggcgtcg
     3001 gcgagttttt ctcccggaga aacgtcgcgg tcgtcgtaaa gaacttccag gccgtcttca
     3061 agcatccgct tgtagatttt cgcggccgat cctt
//
LOCUS       DAOPQT010000067         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_34415_length_3088_cov_2.890867, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000067 DAOPQT010000000
VERSION     DAOPQT010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34415_length_3088_cov_2.890867"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="PKG74_02155"
     CDS             complement(<1..1552)
                     /locus_tag="PKG74_02155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOB90115.1"
                     /translation="MNNKTTKKDEPVRFIPVGGFEEIGRNMMAFEYKEEIILIDMGLQ
                     FPEEETPGIDFIIPNTSYFEPKRQNIKAVILTHAHYDHIGAIPYLSKKLGNPPIYTTA
                     LTKEIINKRQEEFLNAPKLNIRIVKNGDVIKFSEHFEAKFFAVPHNIPDTTAVILRTP
                     AGNFANLADVKIDYDAEGKPTNLEEFRAAAKEKIHVLFLDSTRAEAPGRSLSEKMVEK
                     NLEELFQKAEGRIIIGTFASLLTRIAEIIKIAEKMGKKVAISGRSMKENIEISRNLGY
                     IKPKKGTIVPIEDIHKYKDDKVMVLSTGAQGEPKASLMRISNGEHKYITIKPGDTVIF
                     SSSVIPGNERSVQILKDNLARQGAIVYHSEIVDIHSSGHASQDELRDIIKIMNPKFFV
                     PIHGYFFMRSQNALNGVESGVPKENILLPDNGQVVEITKTSAKITDKTIPASYVLVDG
                     LGVGDVGEVVLRDRRLLAQEGMIVIIATVSRRDGRLVKNPDIISRGFIYLKENQKLLE
                     DIRTKTRGI"
     gene            complement(1617..1701)
                     /locus_tag="PKG74_02160"
     tRNA            complement(1617..1701)
                     /locus_tag="PKG74_02160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1665..1667),aa:Leu,seq:cag)
     gene            complement(1771..>3088)
                     /locus_tag="PKG74_02165"
     CDS             complement(1771..>3088)
                     /locus_tag="PKG74_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOB90116.1"
                     /translation="ISKLVFANLGTLAEIRQENNAKEWVVRLKEDLKWQDKTKITSDD
                     IIFTIETILNPDSHSPLYQSFEGAVVSRVSELETRFTLPAPYVFFETTLKNLEPIPKH
                     IFGNIPALNFRLSNYVLEPIASGPYVFDSYKKNKNGFIEEYRLKVNPDYSPKPYISRF
                     VFKFFTSEAEAIAAYNAGTIDGLTVSDPENLDQIRVRHSVERLAAPRYYAVFINQSLA
                     SSLKDLKARAALDQAIDKQALVENIFKGFAVPIKGPTKDAEPTEVEGLIPEGSTFNLT
                     IADIPVLRKTAEAIKADWQKIGVETNIVPLSITEISESIRKRDYQMILFGNILNNPED
                     FYSFWHSSKRFYPGLNLALYQNSRVDSLIERIRAEVDPGQRLVDVARLGDLIASDFPA
                     IFLYSPDYLYIASPKLKGFESASVITPTDRLENIETWYIKTTRQLK"
BASE COUNT          642 a          702 c          857 g          887 t
ORIGIN      
        1 caatgccgcg tgttttggtg cggatgtctt cgagaagttt ctggttttct ttgagataga
       61 tgaacccgcg ggaaatgatg tccggattct tcacgagccg gccgtcgcgg cgactcaccg
      121 tggcgatgat gacgatcatc ccttcctggg caaggagtct tcgatcacgg agtaccactt
      181 cgccgacgtc gccgacgcca agaccatcca cgaggacata ggaagcggga atggttttat
      241 cggtgatctt cgccgaagtc ttggtgattt ccaccacttg gccgttgtcc ggaagaagga
      301 tgttttcttt gggaacgccg gattcgacgc cgttcaaggc gttttgcgat ctcatgaaaa
      361 agtatccgtg gatcggaacg aagaacttgg gattcatgat tttgatgata tcccggagtt
      421 cgtcctggga agcgtgcccc gaagaatgga tgtcgacgat ttccgaatgg tagacaatgg
      481 ctccttgtct cgccaggttg tctttaagta tctggacgct ccgttcgttt cccgggatga
      541 ccgaagagga gaaaatgacc gtgtctccgg gtttgatggt gatgtactta tgctcgccgt
      601 tcgagattct catcagactt gccttgggtt cgccctgggc gccggtcgag aggaccatca
      661 ccttgtcgtc cttgtactta tggatgtcct cgatcggaac aatggttcct tttttcggct
      721 tgatgtatcc gagattcctg gagatttcga tattttcctt catcgagcgg ccggaaatgg
      781 ccactttttt gcccattttc tcggcgatct tgatgatttc ggcgattctc gtgagtaaag
      841 aggcgaaagt gccgatgatg atccgacctt cggctttttg gaaaagctct tcgagatttt
      901 tctccaccat tttttccgaa agcgaacgtc cgggagcttc ggctctggta ctgtcgagga
      961 aaagaacgtg gattttttcc ttggccgcgg ccctgaattc ctcgaggttg gtcggtttgc
     1021 cttcggcgtc gtagtcgatt ttgacgtccg ccaagttggc gaaattgccg gccggagtcc
     1081 ttaagatgac ggcggtcgtg tccggaatgt tgtgggggac ggcgaagaat ttggcttcga
     1141 aatgttcgga gaacttgatg acgtccccgt ttttgacaat ccggatgttc aatttcggag
     1201 cgttcaggaa ttcttcctga cgcttgttga tgatttcctt ggtgagggcg gtcgtgtaga
     1261 tcggaggatt gccgagtttt ttcgagagat agggaatggc gccgatgtgg tcgtaatggg
     1321 catgggtcag aatgaccgcc ttgatgtttt gtctttttgg ttcgaaataa gaagtgttcg
     1381 ggatgatgaa atctattccc ggggtttctt cttcggggaa ctgaaggccc atgtcgatta
     1441 aaataatctc ttccttgtat tcgaaggcca tcatgttccg gccgatctcc tcgaatcctc
     1501 ccacggggat gaatctcacc ggttcgtctt ttttagttgt tttgttgttc attaaaaatc
     1561 aaactcttga ttggtttttg tcgacttttt ttctgatgta aaaacaagaa tttatttggt
     1621 gccgaagggg agatttgaac tcccaagggt ttccccgcag agccctgaac cctgtgcgtt
     1681 taccaatttc gccacttcgg ctttctcttt tattagaagt tatccaagct tggcttcaga
     1741 atatcagcaa gcggtttttt ccgcaagccg ttatttgagc tgtctcgtgg ttttgatata
     1801 ccaggtttct atgttttcga ggcggtcggt cggggtgatc acagatgcgg attcgaatcc
     1861 tttcagtttc ggcgaagcga tatagaggta atccggagaa tagaggaaaa tggccggaaa
     1921 atccgaagcg atgagatccc cgagcctcgc cacgtcgacc agcctttgac cgggatcgac
     1981 ttcggcccgg attctttcga tcaggctgtc gacccgggaa ttctggtaaa gcgccaggtt
     2041 cagtcccgga tagaatcttt tcgacgagtg ccagaacgaa tagaaatctt ccgggttgtt
     2101 cagaatgttt ccgaaaagaa tcatctggta gtcgcgtttt ctgatggatt cgctgatttc
     2161 cgtaatgctc aagggcacga tgttcgtttc gacgccgatc ttctgccagt ctgctttgat
     2221 cgcttcggcg gttttccgga gaacggggat gtcggcgatc gtcaaattga aagtcgatcc
     2281 ttcggggatc agaccttcga cttccgtcgg ctcggcgtct ttggttgggc ctttgatcgg
     2341 aacggcgaat cctttgaaga tgttttcgac gagagcttgt ttgtcgatcg cctggtccag
     2401 ggccgctctg gctttcaggt ctttcaggct cgaagccaga ctctggttta tgaaaacagc
     2461 atagtatctc ggcgccgcaa gacgctccac cgaatggcgg acccggatct ggtcgagatt
     2521 ttccggatcg gacacggtaa gtccgtcgat cgtcccggcg ttataagcgg cgatggcttc
     2581 ggcttcgctc gtgaaaaact tgaaaacgaa acgcgagata tagggcttcg gcgaataatc
     2641 gggattgact ttcagacgat actcctcgat aaagccgttc ttgttctttt tgtaggagtc
     2701 gaaaacataa ggtccggaag cgatcggctc gagtacgtag ttcgacaatc ggaaattcaa
     2761 agccggaata ttgccgaaaa tatgtttcgg gatcggctcc aggtttttaa gcgtggtttc
     2821 gaagaaaaca tagggcgcag gaagagtgaa cctggtttcg agctcgctca cccgggaaac
     2881 caccgctcct tcgaagcttt ggtaaagcgg ggaatgggag tcgggattca aaatggtctc
     2941 gatcgtgaaa atgatgtcgt cgctcgtgat cttggttttg tcctgccatt tcagatcttc
     3001 tttcaagcgg accacccatt ctttggcgtt gttttcctgt ctgatttcgg cgagcgttcc
     3061 gagattggcg aaaaccagtt tcgagatg
//
LOCUS       DAOPQT010000068         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_34417_length_3088_cov_2.811078, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000068 DAOPQT010000000
VERSION     DAOPQT010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34417_length_3088_cov_2.811078"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PKG74_02170"
     CDS             complement(<1..1029)
                     /locus_tag="PKG74_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90117.1"
                     /translation="MVRIENAVKFIINFISNLVHFTEASYLVVIETIVVGGVLLIIFT
                     ALYRFFKLWWMDTIQTDYLKKLPWMSVEIKVPRENIKSPKSMEQVFANIFGIYSFGLK
                     PEEVVLEGKIEDWISAEIAASHNQIRFIFYFPTKDKNTVQSAIYGQYPDAEITEIEDY
                     TKILPLDLPNDEYDVFGADMILGKRGANNALPMRTYTDFSGGATISRFRFDVEKIDPI
                     AGLLEAMANLKEGEHIWYQILMQPYNSGDFKKLSEEYINKIALKPDIDPATGKQKIDP
                     ITGKKKFIPKRDDELTAEQKEQIRAANAKASKLVFKAIPRFVYIARKSIADRGKFYAL
                     VSSFQIFSV"
     gene            complement(1017..1853)
                     /locus_tag="PKG74_02175"
     CDS             complement(1017..1853)
                     /locus_tag="PKG74_02175"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOB90118.1"
                     /translation="MKQNPLSIEELVEAVIGKTELDDRSRKIVEKRFGLAADKPATLQ
                     ELGNTYGVTRERVRQVEAEALIDLKRAAAKIAETDKLIEFIVDYLSGTGGVRGDDFLM
                     KDIYNLSRPESEEAVFKNKLRFLLQLKDTPHFQKENEDYYDFWYSQEDALATLESVHS
                     SLLRQLKKTEEFLDILRSIIVPQHITEPVALNYISVSKKIGVGPYGDLGLRDWDEINP
                     RTVRAKIYLALKKSGRPLHFTEIASMIESHAPTVHNELIKDRDKFVLVSRGTYGLKQW
                     SE"
     gene            complement(1941..2012)
                     /locus_tag="PKG74_02180"
     tRNA            complement(1941..2012)
                     /locus_tag="PKG74_02180"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1978..1980),aa:Lys,seq:ttt)
     gene            complement(2024..>3088)
                     /locus_tag="PKG74_02185"
     CDS             complement(2024..>3088)
                     /locus_tag="PKG74_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOB90119.1"
                     /translation="QAMDDYYKILGLNRNASEEEIKTAYRKLAHKYHPDKAGGDEQKF
                     KKINEAYQVLSNKEKRQQYDRFGRVFDAGQAGANPFQGFNWNVDFNGADFGNFGGIGD
                     IFETFFGGGRTGYETRRKGVDLEMVKEISLEQAASGTKAEISYSTLVECDKCKGKGYD
                     SSKGVSKCEACKGSGRVKNMRNTILGSFYQESVCPECRGKGSIPKERCSACRGRGAVS
                     GKREVTIDIKPGVENGQIIKVSGVGEALADAPAGDLYVRIRIKPHPVFERRGNDLIMK
                     KSIGFKDILLGKKLEITTLSGKKIDVVIPPGFNLRDDLRVPGEGITRQGDLYIRFEVK
                     TPKKISSDLKKSLEESDGEW"
BASE COUNT          683 a          787 c          727 g          891 t
ORIGIN      
        1 caccgagaaa atctggaaag aagagacaag agcgtaaaac tttcccctgt cggcgatgct
       61 ttttcgggcg atatagacaa atcgcggtat ggccttaaaa accagttttg aggctttggc
      121 gttcgccgcc cggatttgtt ctttttgttc ggcggtcaat tcgtcgtctc ttttggggat
      181 aaattttttc tttccggtta tggggtctat tttttgtttt ccggttgcgg gatcaatatc
      241 gggtttaaga gcgattttat tgatgtattc ctcggacagt ttcttgaaat cgccactgtt
      301 ataaggctgc atcaatatct ggtaccagat atgttcgcct tccttcaggt tggccatcgc
      361 ctccaaaagt ccggcgatcg gatcgatttt ctcgacgtcg aaccggaaac ggctgattgt
      421 ggcgcctccg gaaaaatccg tgtaagtcct catcggcaaa gcgttattcg cgcctctttt
      481 cccgagaatc atatccgccc cgaagacgtc gtattcgtcg ttcggcagat caagcggcag
      541 gattttggtg taatcttcaa tttcggtgat ttcggcatcc ggatactgcc cgtaaatcgc
      601 cgactgcacg gtattcttgt ctttggtcgg aaaataaaaa ataaacctga tttggttgtg
      661 ggaagcggcg atctccgccg agatccagtc ctcgatcttt ccttcgagaa cgacttcttc
      721 cggcttcaaa ccgaagctgt aaatgccgaa gatgttcgcg aaaacctgct ccatggattt
      781 gggagatttg atgttctccc gcggaacttt gatttccaca ctcatccaag gaagcttctt
      841 cagataatcc gtctggatgg tatccatcca ccagagcttg aaaaatcggt aaagcgccgt
      901 gaagataata aggagaaccc cgccgacgac gatcgtctcg atgacgacca agtagctcgc
      961 ctcggtgaaa tgcaccaggt tcgatatgaa attgattatg aatttgacgg cgttttctat
     1021 tctgaccatt gtttcaggcc gtaggttccc cgcgagacca aaacgaattt gtccctgtct
     1081 ttgatgagtt cgttatggac ggtcggggcg tggctctcga tcatcgaagc gatttccgtg
     1141 aaatggagcg gccggcccga ttttttcaaa gccagataga tcttggctct tactgttctc
     1201 ggattgatct cgtcccagtc gcgaagcccg agatcaccgt aagggccgac tccgattttt
     1261 ttggaaacgg aaatgtaatt cagggccacc ggctcagtga tgtgctgcgg aacgattatc
     1321 gaacggagga tgtccaaaaa ttcttcggtt tttttcagct gtcgcaaaag cgaagagtga
     1381 accgactcca aagtcgccaa agcgtcttct tgcgaatacc aaaaatcgta gtagtcttcg
     1441 ttttcttttt ggaaatgcgg agtgtccttc aactgaagca aaaatctcaa tttgttcttg
     1501 aagaccgctt cttccgattc cggacgcgaa agattataga tgtccttcat caagaaatcg
     1561 tctcccctga cgccgccggt tccggaaaga taatcgacga tgaattcgat taatttgtcg
     1621 gtttcggcga ttttcgcggc cgctcttttc agatcaatca aagcttccgc ttccacctgg
     1681 cggacccttt cgcgggtgac gccgtaagta ttcccgagct cctgaagagt ggccggtttg
     1741 tcggcagcca agccgaatcg tttttcgacg attttccggc tccggtcgtc gagctctgtc
     1801 ttcccgatga cagcctcgac caattcttca atgctcaagg gattttgttt catattgtgt
     1861 attataaaat tacaacgttt tcttacccgt gtcaagtaaa ttgtgccgga tcaagcgacg
     1921 gataaataaa atttatcccg tccccccgga aggactcgaa ccttcaactt tttgcttaaa
     1981 aggcaactac tctaccattg agttacgggg ggataagaga atcttaccac tcgccgtccg
     2041 attcttcaag cgatttcttg aggtcggagc tgattttttt cggggttttc acctcgaagc
     2101 ggatatagag atcgccttgc cgggtgatac cttcgcccgg aacgcgaaga tcgtcgcgga
     2161 gattaaatcc cggcgggatg accacatcta ttttctttcc ggataaagtc gttatctcca
     2221 gttttttgcc gaggagaatg tccttgaatc cgatcgattt tttcatgata agatcgttcc
     2281 cgcgtcgctc gaaaaccgga tgcggtttta tccttatcct cacataaaga tctccggctg
     2341 gagcgtcggc caaagcctcg cccacgccgc tcactttgat gatctggccg ttttccactc
     2401 cgggtttgat gtcgatcgta acttcccttt ttccggaaac cgcgccgcgg ccgcggcaag
     2461 cagaacagcg ttctttgggt atgcttcctt ttccgcggca ttccgggcaa accgattcct
     2521 gataaaaact gccgaggatg gtgtttctca tatttttcac ccggccgctg cctttgcagg
     2581 cttcgcattt cgaaacgcct ttcgaagaat cgtatccttt gcctttgcat ttatcgcatt
     2641 cgaccaaagt ggaataagaa atctccgctt tggttcccga agccgcctgt tccaaactga
     2701 tctccttgac catttccaaa tcaactcctt tgcggcgggt ttcgtaaccg gttcgccctc
     2761 ctccaaaaaa cgtctcgaaa atgtctccga ttcctccgaa gttcccgaaa tccgctccgt
     2821 tgaaatcgac gttccagttg aatccctgaa aaggattggc tccggcttgt ccggcgtcga
     2881 aaacccgtcc gaagcggtcg tactgctgcc gcttctcctt attcgagaga acctggtagg
     2941 cttcgtttat tttcttgaat ttctgctcgt ctcctccggc tttgtccgga tgatatttgt
     3001 gcgccagctt gcggtaggcg gttttgattt cctcctcgga agcattacgg ttaagcccca
     3061 aaattttgta ataatcgtcc atcgcttg
//
LOCUS       DAOPQT010000069         2841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_38333_length_2841_cov_3.515075, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000069 DAOPQT010000000
VERSION     DAOPQT010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38333_length_2841_cov_3.515075"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..223)
                     /gene="rpsR"
                     /locus_tag="PKG74_02190"
     CDS             complement(20..223)
                     /gene="rpsR"
                     /locus_tag="PKG74_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOB90120.1"
                     /translation="MERQCFFCSQNMTDVDYKDVELLRRFVSGQAKIIDPKHTGTCAK
                     HQRILAQAVKRARFMGLLPFVRQ"
     gene            complement(236..685)
                     /gene="ssb"
                     /locus_tag="PKG74_02195"
     CDS             complement(236..685)
                     /gene="ssb"
                     /locus_tag="PKG74_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOB90121.1"
                     /translation="MNLNKVFLIGRLTIDPEIRTIPSGEKVTSFGVATNRTWTDKSGN
                     RKEATEFHNVVLWGRQAEIVSQFLKKGSVIFIEGRLQTRSWQGKDGVSRRTTEIIGER
                     VQLGPRAASQGPSEETPEAAPESVEKPEEMPVIDLDEEGIKPEDLPF"
     gene            complement(685..1101)
                     /locus_tag="PKG74_02200"
     CDS             complement(685..1101)
                     /locus_tag="PKG74_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOB90122.1"
                     /translation="MDNEEKRKYEIALLTKDESTALKVADLLSAEGAEIVVGPRPNLI
                     KLAYKIDKEESAFFSFIDFESLPEKIASLDKSLKLDKSILRFLIVTPPPAKAVAKSFG
                     KKPTADSAPKPEAADDTVKESLSNEALEQKLEEILS"
     gene            complement(1147..>2841)
                     /locus_tag="PKG74_02205"
     CDS             complement(1147..>2841)
                     /locus_tag="PKG74_02205"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOB90123.1"
                     /translation="QELGFEYATKSGITWGMSDLVVPEEKKGIIEAANAKVAVIDEQY
                     ESGLLTESERKARVIEIWDNVKTEIEKVAKAALPKSSPVSTIIASGARGSWEQVNQMM
                     GMRGLVKNPQGEIIDLPVTSCYKEGFSPLEYFISVHGNRKGLVDTALKTAEAGYLTRR
                     MIDVSQSLVIKEEDCRTKEGIEIRRSEGLEFGHKFSDRLFSRTSLEEIKSKGKILVKA
                     NEIISIEAAKAIEADPVIEVVKVRSPMTCKTLYGICSKCYGYDLGHNEPIKLGEAVGV
                     IAAQSIGEPGTQLTLRTTHSGGVARADITSGLPRIEELFEVRVPKGKALLSEYDGVID
                     KIEDKGNLRSVFVKVNKKAGAKKDKIVEYQMPRNTLLLIESGQVVSKGDPISEGSIDL
                     KELYVYKGKEEVYRYLIREAQKIYVSEGAAINNKHLEVVLRQMFSRVRISDPGQSQFV
                     IGEVIDKSRFFEVNRDLKKAGKEPARAEELLLGIDKVALSAEGFLSAASFQETARVLV
                     SAASEGKEDPLVGLKENVIIGRLLPIGTSFRGEFENIEEKQEPIAPDTETESAPGE"
BASE COUNT          571 a          775 c          698 g          797 t
ORIGIN      
        1 ggtttgtcga aagcgaacat tattgcctta caaagggcaa aaggcccatg aatctcgctc
       61 ttttcacggc ttgcgccagg attctctggt gcttggcgca ggttccggta tgcttcggat
      121 cgatgatctt cgcctgaccc gagacaaatc ttctcaaaag ctcgacgtct ttataatcga
      181 catcggtcat attctgggaa caaaaaaaac attgcctttc catggtttaa aaaagttaaa
      241 atggtaagtc ttccggcttg atgccttcct cgtccaaatc gatcaccggc atctcttcgg
      301 gtttttcgac cgattccggc gcagcctccg gtgtttcttc cgacggccct tggctcgcgg
      361 cccggggtcc aagctggact ctttcaccga taatctcggt cgtccggcgg gaaactccgt
      421 ccttaccttg ccagcttctc gtctgcagcc gtccttcgat aaaaatgacg cttcctttct
      481 tcaagaactg gctgacgatt tccgcctgcc tgccccaaag aaccacgttg tggaattccg
      541 tcgcctcttt ccggtttccg gatttgtcgg tccaggtccg gttggtggcg acgccgaaag
      601 aagtcacttt ctctccggaa ggaatggtcc tgatttccgg gtcgatcgta agccttccta
      661 ttaagaaaac cttgttgaga ttcattaaga caaaatttct tcgagttttt gttccagagc
      721 ctcgttgctc agcgactctt tgacggtgtc gtcggccgcc tccggtttgg gagcggaatc
      781 ggccgttggt tttttgccga acgatttcgc caccgctttg gccgggggcg gagtaacgat
      841 caaaaatctc aagatcgatt tgtcgagttt gagggatttg tccaaagaag cgatcttctc
      901 cggcaaggac tcgaaatcga taaaagagaa aaaagccgat tcttccttgt cgatcttgta
      961 agccaacttg atgagattgg gcctagggcc gacaacgatc tccgcgcctt cagcggagag
     1021 caaatcagcc actttcaggg cggttgactc gtctttcgtc aaaagcgcta tttcgtattt
     1081 tcttttctct tcgttatcca tttataaaca ttgatagcct aacttatcgg ctccagtatt
     1141 gtcaatttac tctcccggag cggattcggt ttccgtgtcc ggagcgatcg gctcttgttt
     1201 ttcttcgata ttctcgaatt cgcccctgaa gctcgtgcct atcggcagca atcggccgat
     1261 gatgacgttt tctttaaggc cgaccaaagg atcttctttg ccttcggaag cggccgagac
     1321 caaaaccctt gccgtttcct ggaacgaggc ggcggacaag aatccttcgg ccgagagcgc
     1381 caccttgtcg attcccaaaa gcagttcttc ggcccgggcc ggctctttgc cggctttttt
     1441 caaatcgcga ttcacctcga agaatctcga cttgtcgatc acttctccga tgacgaattg
     1501 gctctgaccc ggatcggaaa tgcgaacgcg ggagaacatc tgccgcaaaa ccacttccaa
     1561 atgtttgttg ttgatggccg ctccttcgga aacgtaaatc ttctgggctt cgcggatcag
     1621 ataacgatag acttcttcct tgcctttgta gacgtaaagt tctttcaagt cgatactgcc
     1681 ttcggaaata ggatctccct tcgagaccac ttgtccggat tcgataagga gcaaagtatt
     1741 cctcggcatt tggtattcga cgatcttgtc ttttttggcg ccggcttttt tgttcacttt
     1801 gacgaaaacc gaacgcagat tgcctttgtc ttctatcttg tcgataacgc cgtcgtattc
     1861 ggaaagcaaa gccttgccct tggggacacg cacctcaaaa agctcttcga tcctcggcag
     1921 tcccgaagtg atgtccgcgc gggcgacacc gccggaatgg gtggtccgga gagtaagctg
     1981 ggttcccggt tcgccgatcg attgggcggc gatgacgccg accgcttctc caagcttgat
     2041 cggctcgttg tgtcccaaat cgtatccgta acacttcgaa caaatgccgt agagagtctt
     2101 gcaggtcatc ggggaccgga ctttgacgac ctcgatcacg ggatcggcct cgatcgcttt
     2161 ggccgcctcg atcgagatga tctcgttcgc tttgacgaga atctttcctt tggattttat
     2221 ttcttccaaa ctggtgcgcg agaaaagcct gtcggagaac ttgtgtccga attcgagtcc
     2281 ttcgctgcgc ctgatctcga tgccttcctt ggtgcggcaa tcctcttctt tgatcaccag
     2341 actttgactg acgtcgatca tgcggcgggt gagatatccg gcttcggcgg ttttcaaggc
     2401 ggtatcaacc agtcctttcc tgtttccgtg gaccgagatg aaatactcga gcggcgaaaa
     2461 tccttctttg tagcaggagg tgaccggcag atcgatgatt tctccctgcg ggtttttgac
     2521 aagccccctc atacccatca tctggttcac ctgctcccag ctgcctctcg cacccgaagc
     2581 gatgatggtc gagaccggac tcgatttggg caaagccgcc ttggccactt tttctatttc
     2641 ggttttgacg ttatcccaga tttcgatgac gcgggctttt ctttcacttt cggtcaaaag
     2701 accgctttcg tattgttcgt cgataaccgc cactttggcg tttgccgcct cgatgattcc
     2761 tttcttctct tcgggcacca ccaagtcgga catgccccag gtgattcccg atttggtggc
     2821 gtattcgaat ccgagctcct g
//
LOCUS       DAOPQT010000070         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_39736_length_2764_cov_2.676633, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000070 DAOPQT010000000
VERSION     DAOPQT010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39736_length_2764_cov_2.676633"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..547)
                     /locus_tag="PKG74_02210"
     CDS             complement(65..547)
                     /locus_tag="PKG74_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90124.1"
                     /translation="MSLKKQKKVKERKPERSGSAKDDLIRFLSDKARKYHALRRLVDD
                     VILKQARQMEDLSREISRTKADLLNGMKIAEISEIPFRGGRYVFARSRSFSIKDPIAL
                     DRWARKNGFVKADQGLIREGLKIADEKNLLPPFAVKVETDSIIYRSEKRKDKKSHGKK
                     "
     gene            complement(537..1649)
                     /locus_tag="PKG74_02215"
     CDS             complement(537..1649)
                     /locus_tag="PKG74_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOB90125.1"
                     /translation="MNFEIRKAERTKSKLRLAIAGPAGSGKTMGSLRVAKGMGGRVCL
                     IDTERGSGDLYADLFPYDIISLTPPFKPDILIAALHAAEAAGYDTIIVDSLSHFWSDE
                     GGLLDQADKLQQAGKNRFTLWADITPQHRRLVNALLNSPKDIIATMRSKQEYAIEQVD
                     GPNGTKRTEVKKLGLAPVQREGMEYEFTIFFDTDQNHNTKASKDRTNMFANEVFILDE
                     SIGARIKKWLETGKLDPRKIRAEIVSQLKDRLEFGMPTNQQDAAEFIRFAIKRLTNLE
                     PTEENYPEILKALSAITDKQAAIDKVYKPADVINPDEIKGPDESKKPEGGEKVGETAK
                     DASQVEGTTNKGISEPAPVAQTAPAPAPAATSQHVA"
     gene            complement(1691..1930)
                     /locus_tag="PKG74_02220"
     CDS             complement(1691..1930)
                     /locus_tag="PKG74_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90126.1"
                     /translation="MEKSSAPVETMRRFEVIQDLRYFAHPSFYHSLLSWSTPRLKALL
                     VYYREVAKEGNSGEIGNQNPIPFMEIRTQISVLRK"
     gene            complement(2158..2712)
                     /locus_tag="PKG74_02225"
     CDS             complement(2158..2712)
                     /locus_tag="PKG74_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOB90127.1"
                     /translation="MKLSKRIAVDSVVAVILILLFGLAHQVDAPKISPDLVCDLGTGK
                     IEKSIPEDTRAARIDAYFSNRKMPAAGLGSVFIAAADRYEIDWSLLPAIAVVESSGGL
                     HACGNNLFGWASCRPGIGEFESIESAIDFVAKNLGGKNPKTARIYGGNIDSDLWAYNG
                     SVNPEYPGKVKKIMGDIERTPLPR"
BASE COUNT          576 a          714 c          707 g          767 t
ORIGIN      
        1 tcctgtccaa tactctttct gattccgttc ggagatcttc ctccaaagcg ccccgatcga
       61 ctgatcattt tttgccatgc gattttttat cctttctttt ttcggaccga taaatgatcg
      121 agtccgtttc gacttttacg gcaaacggcg gaagcaagtt tttttcgtcg gcgatcttga
      181 gtccttcccg gatgagtcct tgatcggctt tgacgaatcc atttttccgc gcccatcgat
      241 cgagagcgat cggatctttg atgctgaagg accgggaccg ggcgaaaaca tatcttccgc
      301 cgcggaatgg gatctccgag atctcggcga tcttcatgcc gttcaagaga tcggctttcg
      361 tccgggaaat ctcccgagaa agatcttcca tctgccgggc ctgtttgagt atgacatcat
      421 cgacaagcct ccggagcgcg tgatattttc gcgctttgtc cgatagaaat cggatcagat
      481 cgtctttcgc ggagccggat ctctccggct tccgctcctt gacttttttc tgttttttaa
      541 gcgacatgtt ggctcgtggc cgccggagcc ggggccgggg ctgtttgggc caccggagcc
      601 ggttcagata tgcctttatt ggtcgttcct tccacttgag acgcgtcttt ggccgtctcc
      661 ccgacctttt cccctccctc aggttttttg ctttcatccg ggccttttat ctcgtccgga
      721 ttgataacat ccgccggctt ataaacttta tcgatggccg cctgtttatc cgtgatcgct
      781 gaaagtgctt tcaagatctc cggataattt tcttctgtcg gctcgagatt cgtgagccgc
      841 ttgatcgcga agcggatgaa ttcggccgcg tcttgctgat tcgtcggcat tccgaattcg
      901 agacgatctt tcaattgaga gacaatctcg gcgcggatct tgcgcggatc cagtttcccg
      961 gtttcgagcc acttcttgat tcgagcgccg atcgattcat cgagaatgaa aacttcgttc
     1021 gcgaacatat tggtccggtc cttcgatgcc ttcgtgttgt gattctgatc ggtatcgaag
     1081 aagatcgtga attcgtattc catgccttcg cgctgaacag gagcgagtcc gagttttttg
     1141 acctcggtcc ttttcgttcc gttcggtccg tccacttgct ctatcgcata ttcttgtttt
     1201 gagcgcatgg tcgcgatgat gtccttcgga gaattcagaa gggcattgac tagccttcga
     1261 tgttgcggcg taatatcggc ccaaagcgtg aagcgatttt tgccggcctg ttgaagtttg
     1321 tcggcttgat cgagcaagcc gccttcatcg gaccaaaaat gagaaagcga gtcgacgatg
     1381 atcgtgtcat agccggcggc ttcggccgca tgaagcgcgg cgatcagaat gtccggcttg
     1441 aacggcggag tgagagagat gatgtcataa ggaaaaagat cggcatatag atcgcccgat
     1501 cctctttccg tgtcgatgag acaaacgcgg ccgcccattc ctttggcgac tctcaatgat
     1561 cccatcgttt tgccggaccc ggccgggccg gcgatcgcga gacgaagttt tgatttcgtt
     1621 cgctctgctt tgcgaatttc aaaattcatg atgatttttt ctttctgcga tccgtggatc
     1681 gcgttagcgg ttattttcgg aggaccgaga tctgcgtccg aatttccatg aatggaatcg
     1741 gattttgatt tccgatctct ccggaatttc cttctttcgc cacctcccga taatagacga
     1801 ggagggcttt gaggcgcgga gtcgaccatg aaagaagtga atggtagaac gacggatgcg
     1861 cgaaatatcg gagatcttga ataacctcga atcgccgcat cgtctcgact ggcgctgatg
     1921 atttttccat aagcggataa tgaattttaa tttttaattt tgaaagatct tcgacctttg
     1981 acttaattat agcgcaagag aaaaagcgcg acaatgagtc aattgtggat aacttcggcc
     2041 gtctccggca ttttacggca tacgaaaaaa gccgggggta aaacccggcc ggggacctct
     2101 cccctaccct atcaaaacga attttttgga ccatttaaga ggggaacacc cgaggcccta
     2161 tcgggggagg ggagtccgct caatgtcgcc catgatcttt ttgaccttcc cgggatattc
     2221 cggattgacc gatccgttgt aagcccaaag atccgagtcg atgtttccgc catagatccg
     2281 cgcggttttt ggattttttc cgccgagatt tttggcaacg aaatcgatcg ctgattcgat
     2341 cgattcgaat tctccaatcc cgggccggca cgaggcccat ccgaaaagat tgtttccgca
     2401 agcgtgaagt cctccggatg attcgacgac cgcgatcgcc ggcaagagcg accagtcgat
     2461 ctcatatcga tcagccgccg caatgaaaac agatccgagt ccggccgccg gcatttttcg
     2521 attcgaaaaa taagcatcga tccgggcggc tctcgtatct tccggaattg atttttcgat
     2581 cttcccggtt ccgagatcgc aaacaagatc cggggagatc tttggcgcgt cgacttgatg
     2641 agcgaggcca aacaataaaa tcagaatgac ggccacgacc gagtcgactg ctattctttt
     2701 tgagagtttc ataatgagcc ggcgttcgct ccggcgactc tgttccgatc gatcgcgctt
     2761 ttac
//
LOCUS       DAOPQT010000071         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_40048_length_2750_cov_3.246011, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000071 DAOPQT010000000
VERSION     DAOPQT010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40048_length_2750_cov_3.246011"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKG74_02230"
     CDS             complement(<1..403)
                     /locus_tag="PKG74_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOB90128.1"
                     /translation="MPFEISTERFSGPLQKLLELIEERKLEITELSLAEITGEFLDYV
                     HKMGEAEPKVLADFVSVAARLLLIKSKSLIPNLELTTEEEESIVDLEERLKLYKELKS
                     AEKNVAVFWKSEKRSFSRDFAAGSRFVVFYPP"
     gene            complement(447..>741)
                     /locus_tag="PKG74_02235"
     CDS             complement(447..>741)
                     /locus_tag="PKG74_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90129.1"
                     /translation="AHANAKMRFYGLSEYRVKRVINHPQRIEKGIAENTAAVMQSSGS
                     KKHPYEIWTMIKDESRSGEKIRKVISAWKYPGTTKPGEPLPQEILIEIKEAGF"
     assembly_gap    742..751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(780..1994)
                     /locus_tag="PKG74_02240"
     CDS             complement(780..1994)
                     /locus_tag="PKG74_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOB90130.1"
                     /translation="MRKILFIIFLGALTAGAFLFLNTTYTFRVGVGAANEPEKPAGVS
                     GIIGKLITIGEEQQAVIGSKNDIENQQPLAEKPTAVKAVYSTAWSAGSAKKIDYFIDL
                     IKTTELNAIVIDIKDYSGYVAYDIGDPLVNKYKAKDIKIPKINSLIKKLHDQGIYVIA
                     RIAVFQDPILAKNRPDLAVQSKSTGTTWFDRQNLAWTDPASLEVWDYNLAIAKDAANR
                     GFDEINFDYIRFPSDGDIADMVFPVYDMTSPKHFTMRKFFEYTRNSLPETPISADLFG
                     LAGIVNDDLGIGQVLEDALPYFDAVSPMAYPSHYAANFMGFKNPAEHPYEVVRNTMAT
                     AMARLHKLEEQGMENKPKLRLWLQDFNMGATYDAVMVRKEIQAVEEIDPTAGWMLWNP
                     NNVYTKGALLPG"
     gene            2052..>2750
                     /gene="dnaX"
                     /locus_tag="PKG74_02245"
     CDS             2052..>2750
                     /gene="dnaX"
                     /locus_tag="PKG74_02245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOB90131.1"
                     /translation="MSLAIYRKYRPKTLTDVLGQESVTEVLKNAAKQDKLSHAYLFYG
                     PRGSGKTTAARILAKIVNCETRMNDPKFRELGEPCNRCRPCLEIDEGRALDVIEIDAA
                     SNRGIEEIRNLKEGIKLSPASYRRKVFIIDEVHQLTKEAFNALLKTLEEPPEHAIFIL
                     ATTEYEKLPATIISRTQRFNFRRLSVKTILGKLQTIVKEEKLKISDDALELVAVAAEG
                     SFRDAESLLDQIISL"
BASE COUNT          644 a          703 c          670 g          723 t
ORIGIN      
        1 ccggcggata aaaaacaaca aatctggagc cggcggcaaa atcccgagaa aacgatcttt
       61 tctccgactt ccaaaaaacg gctacgtttt tttccgcgga tttgagttcc ttgtaaagct
      121 tgagtcgttc ttcgagatcg acgatgcttt cctcttcttc ggtcgtcagt tccagattcg
      181 ggatcaggct tttcgatttg ataagcaaaa gcctggcggc cacggaaacg aaatcggcca
      241 ggactttcgg ttcggcttcg cccattttat gcacgtaatc caaaaattct ccggtgattt
      301 ccgcgaggct caattcggtg atctcgagct tgcgttcttc gatgagttcc aggagcttct
      361 gcaacggacc ggagaatctt tcggtgctga tttcgaaagg cattttacga attataaccc
      421 cgaaaccttg ccccgccaaa aacgcttcag aatccggctt cctttatttc gatcaggatt
      481 tcttgcggca gcggctctcc gggtttggtt gttcccggat atttccaggc ggaaatgact
      541 ttcctgattt tttctccgga tcgggattcg tctttgatca tcgtccagat ctcgtaagga
      601 tgctttttgc tgccggagga ttgcatcacg gccgcggtat tttcggcgat ccccttttcg
      661 attctttgtg gatggtttat gacgcgtttg acgcggtact cggaaaggcc gtaaaaacgc
      721 attttcgcgt tcgcgtgagc tnnnnnnnnn ncaaaccagt tttttccggc cgagcatgtt
      781 tatcccggaa gcaaagcgcc cttggtatag acgttattcg gattccaaag catccagccg
      841 gccgtcgggt ctatctcttc gaccgcctgg atctcttttc tcaccatgac ggcgtcatag
      901 gtcgcgccca tattgaagtc ctgcagccaa agacgaagct tgggtttatt ctccatgcct
      961 tgttcctcga gcttatggag tcttgccatg gccgtagcca tggtatttct taccacttcg
     1021 taaggatgtt cggccggatt cttgaatccc atgaaattcg cggcatagtg cgatggataa
     1081 gccatgggcg aaaccgcgtc gaaataaggc aaggcgtcct cgagcacttg gccgataccg
     1141 agatcgtcgt tcacgattcc cgccagcccg aaaagatcgg ccgagatcgg tgtttccgga
     1201 agactgtttc tcgtgtattc aaaaaacttt ctcatggtga agtgcttcgg gctcgtcatg
     1261 tcgtaaaccg gaaaaaccat atcggcgatg tcgccgtccg aaggaaagcg gatgtaatcg
     1321 aaattgattt cgtcgaatcc ccgattggcc gcgtctttgg cgatcgccag attgtaatcc
     1381 cagacttcca gcgaagccgg gtctgtccag gccagatttt ggcggtcgaa ccaggtggtt
     1441 ccggtcgatt tgctctggac cgccaaatca ggcctgttct tggccaaaat cgggtcctgg
     1501 aacacggcga tacgggcaat gacgtaaata ccctggtcat gcaatttctt tatcagggag
     1561 ttgattttgg ggattttgat gtctttggcc ttgtatttgt tgacgagtgg atcgcctatg
     1621 tcgtaagcga cgtaaccgga ataatccttg atgtcgatga caatggcgtt cagttccgtg
     1681 gttttgatga ggtcgatgaa ataatcgatt tttttggccg agccggctga ccaggcggtc
     1741 gagtaaaccg ctttgacagc tgtcggtttt tccgcaaggg gttgttggtt ttctatgtcg
     1801 ttcttcgatc cgatgaccgc ctgttgttct tccccgatcg ttatcagttt tccgatgata
     1861 cccgaaactc cggccggttt ttccggttcg ttcgccgccc caacaccgac tctaaaagtg
     1921 taagtcgtgt tcaaaaacaa aaaagccccc gcggtcaagg ctcccaaaaa gattatgaaa
     1981 aggatttttc tcatcaaact ccggcttttg aagcgttttt attctaatcc attttcacct
     2041 ataataaaag catgtctctc gccatttacc gcaaataccg cccgaaaacc ttaaccgatg
     2101 ttttgggcca ggaatcagtg accgaagtcc ttaagaatgc cgccaaacag gacaagttga
     2161 gtcacgccta tcttttttac ggaccccgcg gatccggcaa aaccacggcc gcgagaatcc
     2221 tcgccaaaat cgtgaactgc gaaaccagaa tgaatgatcc gaaattccgg gaactcggag
     2281 aaccttgcaa tcgttgccgg ccttgcttgg aaatcgacga aggccgagcc ttggacgtca
     2341 tcgagatcga cgccgcttcg aaccgcggca tcgaagaaat ccggaatctc aaagaaggca
     2401 ttaagctttc cccggcttcc taccgccgga aggtattcat catcgacgag gttcatcaac
     2461 taaccaaaga agctttcaac gcacttttaa agactttgga agagccgccg gagcacgcca
     2521 tatttatttt ggcgacgacc gaatacgaaa aacttccggc gacgatcatt tcccggaccc
     2581 aacgtttcaa tttccgaaga ttgtccgtga aaacgatcct cggaaaactt cagacgatcg
     2641 tcaaagaaga aaaactgaaa ataagcgacg acgctctgga actcgtggcc gtggccgccg
     2701 aaggaagctt cagggacgcg gaatctttac tcgaccagat aatttctctc
//
LOCUS       DAOPQT010000072         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_40197_length_2741_cov_4.396500, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000072 DAOPQT010000000
VERSION     DAOPQT010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40197_length_2741_cov_4.396500"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..>2741
                     /locus_tag="PKG74_02250"
     CDS             62..>2741
                     /locus_tag="PKG74_02250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOB90132.1"
                     /translation="MSSAKTKNFSSHPSSFVKEPDLIAVQIDSFEWFKKTGLRNLFDE
                     ISPIRDHSAKEMELYFRDYYFDEPKFSTDECRERALTYEAPLRVNMELVHLVSGRKET
                     QEVYFGDFPIMTEKGTFIINGVERVVILQLVRSPGVYFISESWRGRKLFGAKIIPNRG
                     AWIEFETDSNGVIDVKIDRHRKMPATSFLRIFGLETEEEIKAVFPNDNGPVKHIENTL
                     KKDPAKTTSESYLELYKRLRPGDLATAENAKNLIDAMFIRSDRYDLSEIGRFKVNQRL
                     ETNDSKDRLLSQKDIVRIMAEIIRLNNDPHAKDDDIDHLGNRRLRSVGELLEARMRLG
                     FGRIRRIVQDRMSTISGEERQALTPIQLVNTRPLIGVVRDFFATSSMSQFMDQVNPLT
                     ELEHKRRISALGPGGLTKERAGFEVRDVHPSHYGRLCPIQTPEGQNIGLINHLANYTR
                     VNKYGFLEAPYVKMEKGKITGEIVWFDAIEEEKHKIVHAGEKLTEDNKLADKIVEVRF
                     QGKPMNVNRDDVEYMDVSPSEILSVGASLIPFLENDDAKRALMGSNMQKQAVPSIRPE
                     APYVGTGVEKTAAYNSGQVIVADEAGEVTYVDGSKIIIEDVDRHKRIYPIDKFRMTNQ
                     YIAFSHKPIVKMGDKVKKGEVIADGTAIKDGVLALGQNLLVAFVSWEGANFEDAIILS
                     ERVAQMDRYTSINIEDFVCDVRDTKLGPEITTPDIPNVSEEKLKNLDEEGIIRIGAEV
                     TAGDILVGKISPKGESELTSEERLLRAIFGEKARDVKDSSLEVPHGKGGRVVGVKIFD
                     REKGDKLEAGVIRRIQIQVASLRSIQAGDKMAGRHGNKGVISQIRPIEDMPYLEDGTP
                     VDIILNPLGVVSRMNLGQILETHLGWAAEKLGYRANT"
BASE COUNT          751 a          677 c          729 g          584 t
ORIGIN      
        1 atattgttaa atatctaaaa taaatcgaga cgaagccgat ttcttaaagg cggtttttta
       61 tatgtcgtca gcaaaaacca aaaacttttc atcccatccg agttcgttcg tgaaggaacc
      121 ggacctgatc gccgtccaaa tcgattcctt cgagtggttc aagaaaaccg gattgcggaa
      181 cctgttcgac gagatctctc ccattcgcga ccattcggcg aaggaaatgg agctttattt
      241 tcgcgattat tatttcgacg aaccgaaatt ttcgaccgac gaatgtcgcg aacgggcttt
      301 gacttacgaa gctccgcttc gggtcaatat ggaattggtc catctcgtgt ccggtcggaa
      361 ggagacccaa gaagtatatt tcggtgattt cccgatcatg accgagaagg ggacctttat
      421 catcaacggc gtggaaaggg tggttatttt gcagctagtg agatcgcccg gagtttattt
      481 catatccgaa tcctggaggg gtcggaagct ttttggcgcc aaaatcattc cgaatcgtgg
      541 cgcctggata gaattcgaaa ccgattcgaa cggcgtgatc gacgtcaaaa tagaccgcca
      601 ccgcaaaatg ccggccacct cttttctgcg tatctttgga ttggaaaccg aggaagagat
      661 caaggccgtt tttccgaacg acaacggtcc ggtcaaacat atcgaaaata ctttaaagaa
      721 agatccggcg aaaaccacct ccgaaagcta tctcgaattg tacaaaaggc tccgccctgg
      781 ggatctcgcc accgccgaaa acgccaagaa tctgatcgac gccatgttca tccgttccga
      841 ccgctacgat ctttccgaga tcgggcgctt caaagtgaac cagcgtctgg aaacgaacga
      901 ttccaaagac agattgcttt ctcaaaaaga catcgtgcgg ataatggcgg aaatcatcag
      961 gctgaacaac gacccgcacg ccaaagacga cgatatcgac catctgggaa accgccggct
     1021 tcgttcggtc ggagaacttt tggaggcgcg gatgcgtttg ggattcggaa gaatcaggag
     1081 aatcgtccag gacaggatgt cgacaatctc gggagaggag cggcaggcgc tcactccaat
     1141 ccagctcgtg aacacccggc ctttgatcgg cgtggtccgc gatttcttcg ccacttcctc
     1201 catgtcgcag ttcatggacc aggttaatcc tttgaccgag cttgaacaca aaaggaggat
     1261 ttcggctttg ggtccgggcg gactcaccaa agaacgagcc ggattcgagg tgagggacgt
     1321 ccacccttcg cactacgggc ggctttgccc gatccagact ccggaagggc agaacatcgg
     1381 acttatcaat catttggcga actatacccg cgtcaacaaa tacggatttt tggaagcgcc
     1441 ttacgtcaaa atggaaaagg gaaaaatcac cggagaaatc gtttggttcg acgcgatcga
     1501 agaggaaaag cacaagatcg tccacgccgg agaaaaactg accgaagaca acaagttggc
     1561 ggacaaaatc gtcgaagtcc gatttcaagg gaagccgatg aacgtcaatc gcgacgacgt
     1621 cgaatacatg gacgtttctc cttcggaaat cctttcggtc ggagcgtctt tgatcccgtt
     1681 tttggagaac gacgacgcca aaagggcttt gatgggctcg aacatgcaaa aacaggccgt
     1741 gccttcgatc aggccggaag cgccctatgt cggcaccggc gtcgaaaaaa ccgccgctta
     1801 caattccgga caggtgatcg tggccgacga agcgggcgag gtgacctatg tcgacggatc
     1861 gaaaatcatt atcgaagacg ttgaccgtca caaacggatt tatccgatcg ataaattcag
     1921 gatgacgaac caatacatcg ctttttctca caagccgatc gtcaaaatgg gcgacaaggt
     1981 taaaaaagga gaagtgatcg ccgacggcac ggccatcaag gacggcgtcc tcgctctggg
     2041 gcaaaaccta ctcgtggctt ttgtttcctg ggaaggagcg aatttcgaag acgccatcat
     2101 tctttcggaa agagtggccc agatggatcg ctacacctcc atcaatatcg aagattttgt
     2161 ctgcgatgtt cgcgacacga aattgggacc ggaaatcacc acgccggaca ttccgaatgt
     2221 ttccgaagaa aaactgaaaa atctggacga ggaaggcatc atcaggatcg gcgccgaagt
     2281 gacggccggg gatattctgg tcggcaagat ttctccgaaa ggagaatccg aactgacttc
     2341 ggaagagcga ctgctccggg cgatcttcgg agaaaaagcg agggacgtca aagattcttc
     2401 tctggaagta ccgcacggaa aaggcggccg cgtagtcgga gtaaaaatct tcgaccgcga
     2461 aaaaggagac aagcttgaag ccggagttat tagaaggatc cagatccaag tggcttcgct
     2521 tagaagtatc caggccggag acaaaatggc cggccggcac ggaaataagg gcgtcatttc
     2581 gcagatcagg ccgatcgagg acatgcctta tctcgaagac ggcacgcctg tggatatcat
     2641 tcttaatcct cttggtgtgg tttcccgcat gaacctcggc cagattttgg aaacacattt
     2701 gggctgggcc gcggaaaaat tgggataccg cgcgaatact c
//
LOCUS       DAOPQT010000073         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_41000_length_2701_cov_3.001134, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000073 DAOPQT010000000
VERSION     DAOPQT010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41000_length_2701_cov_3.001134"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..567
                     /locus_tag="PKG74_02255"
     CDS             136..567
                     /locus_tag="PKG74_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOB90133.1"
                     /translation="MKIRIKRFDKSLSLPEYKTSGAGAFDLTSRERMEIDPGEIGYIP
                     LNVAIETPEGFVTLLVARSSLHKKGLWLANGLGVIDADFCGDEDELKAVCFNFTDKPI
                     MVEKGDRVCQAIILKREKAEWEEVDRMAGKTRGGFGTTGEK"
     gene            604..674
                     /locus_tag="PKG74_02260"
     tRNA            604..674
                     /locus_tag="PKG74_02260"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:636..638,aa:Pro,seq:cgg)
     gene            738..1658
                     /locus_tag="PKG74_02265"
     CDS             738..1658
                     /locus_tag="PKG74_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOB90134.1"
                     /translation="MPRKPKKEDKKESGLNVRVIKAEPENEKIEEPAVSAEETVLFLK
                     KHAVSACPEPETNDKPDDGYEDWEDNEPNNRKKRKLSIITRTVVFLFMVAAVWFGLKL
                     ANKSGYSACLSDNGVDAGNCGVALQKLKDDQVIDKTRLADAGSIIAGLQKYSLEKRAL
                     PENLSTLLSGGYIPKTYSDPEFDKPYFYKRQNNTSYSFCVYLSSGAWGVNPAGCPSRE
                     SYLSGIYEEETPPVPETRTVTVQETSIGWLNVRQSPTTDSSIITKAYAGEQYRVLEEK
                     GDWLRIPLKSSVLINGEQVIEGWVYKSYVK"
     gene            1752..2054
                     /locus_tag="PKG74_02270"
     CDS             1752..2054
                     /locus_tag="PKG74_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90135.1"
                     /translation="MLFIALIHLTGSILGAVMAVFNYRQSRTLIGSFFKRYYQLTVLA
                     STLLAIGFAGKCFVLDKPGSVADGLVQFILLAAAGIYAMAIIVWPKEANKYLSCKL"
     gene            2118..>2701
                     /locus_tag="PKG74_02275"
     CDS             2118..>2701
                     /locus_tag="PKG74_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOB90136.1"
                     /translation="MKISFFEIEDWEAEYLKSKLPGHELFFSPNRIDKNNLTEAGNFD
                     VISVFTGSKIDDPVLEALPNLKLIATRTTGFDHIDLVATAAKNIVVSNVPSYGENTVA
                     EHAFALLLSISRKIFQSFDRIKETGSFDQKGLQGFDLKGKTIGVVGVGRIGQYAIRIA
                     KGFGMDVIAYDAFPRAGLEAELGFRYVSFEELLK"
BASE COUNT          757 a          633 c          709 g          602 t
ORIGIN      
        1 cgaaaaacgc gccgaagaat tgaacgagct atccgacaaa gaacttgcgg ccatggccga
       61 caaagccaaa gaagccaaac aggaattcga ggaggggatc gaatcggaga tgaagaaaag
      121 acactacgtg aaataatgaa gatccgcata aaaagattcg acaagagtct gtctcttccg
      181 gaatacaaaa cgagcggcgc cggagctttt gatctgacga gccgcgaacg tatggaaatc
      241 gatcccggag agatcggcta tattcctttg aatgtcgcga tcgaaacgcc cgaaggattc
      301 gttactcttc ttgtcgcccg gagttcgctc cacaaaaagg gcttgtggct tgcgaacggc
      361 ttgggcgtca tcgatgcgga tttttgcgga gacgaggacg aactgaaagc ggtttgtttc
      421 aatttcacgg ataagccgat aatggtggaa aaaggcgacc gcgtctgcca ggcaatcatt
      481 ttgaaacgcg aaaaagccga atgggaagaa gtcgatcgca tggcggggaa aacccgggga
      541 ggattcggaa ccaccggcga aaagtagacg aaaagcgctt tttgcgatta aataggatag
      601 tcgcgggctg tagtataccg gtagtacgtg cgcttcgggt gcgtaaagac cgagttcgat
      661 tctcggcagc ccgacaatcg aggttcatcc acaatttctt ttcgacaaag cgagaatgaa
      721 atataataat aagtactatg cccaggaagc ctaaaaaaga agacaaaaaa gaaagcggat
      781 tgaatgtccg cgtaatcaaa gccgagcccg aaaacgaaaa gatcgaagag ccggcggttt
      841 ccgccgaaga aacggtcttg ttcctgaaaa aacacgccgt gagcgcttgc cccgaaccgg
      901 aaacaaacga caagccggac gacggctacg aagattggga ggacaacgag ccgaacaacc
      961 gcaaaaagag aaaattgtcg ataataacga gaaccgtcgt tttcctgttt atggtcgcgg
     1021 ccgtctggtt cggactgaaa ctggccaaca aaagcggata cagcgcctgc ctttcggaca
     1081 acggagtgga tgccgggaat tgcggcgtcg ctctacagaa actgaaagac gatcaggtta
     1141 tcgacaagac aagactggcc gatgccggaa gcatcatcgc cggcctccaa aaatacagcc
     1201 tcgaaaaaag agctttgccg gaaaatctgt cgactctttt gtccggcgga tacattccca
     1261 aaacttacag cgatcccgaa ttcgacaaac cctatttcta caagagacaa aacaacactt
     1321 cttacagctt ttgcgtttat ctttcttccg gggcctgggg agtgaatccg gccgggtgtc
     1381 cgagcaggga aagttatttg agcgggattt acgaagaaga gacgccgccg gtgccggaga
     1441 caagaaccgt taccgtccaa gaaacgtcga tcggctggct gaacgtgcga cagtctccga
     1501 ccacggattc gtcgatcatc accaaggctt atgccggaga acaataccgg gtgcttgaag
     1561 aaaaaggaga ttggcttcgc attccgctga agtcatcggt acttataaac ggcgaacagg
     1621 tgatcgaggg ttgggtttac aagagctacg tcaaataacg cctggttttg tggtataatt
     1681 cgtcaaaggt tgaaagccgt gtccgcggac gggttctcta tccttgtccg cggaggcgga
     1741 aaaatataaa aatgttattt atcgccctaa ttcatttgac gggatctatt ctcggagcgg
     1801 taatggctgt tttcaattat cggcagagtc ggactttaat cggatctttt tttaagagat
     1861 actaccaact gacagttctt gcctcaacac tcctcgctat cggattcgcc ggaaagtgtt
     1921 ttgttttgga taaacccgga agcgtcgccg acggcttggt gcagttcatt cttttggccg
     1981 ccgccggcat ttatgccatg gcaatcatcg tttggccgaa agaagccaac aaatatctga
     2041 gctgtaagct ttaatctatc tggaagcgct ttatcaaagc cttggtaaaa ggcgcgtttt
     2101 cgcctaaact taatcttatg aagatttctt ttttcgagat cgaggattgg gaagcggaat
     2161 atctgaaatc caaacttccc ggacacgaac tttttttctc cccgaaccgg atcgataaaa
     2221 acaatctcac ggaagccggg aatttcgacg tgatttcggt tttcaccggc tccaaaatcg
     2281 atgatccggt tctcgaagct ttaccgaatc tgaaactgat cgccacccgg accacgggat
     2341 tcgaccatat cgatcttgtc gctaccgcgg cgaaaaacat cgtcgttagc aatgttccgt
     2401 catacgggga aaacaccgtc gccgagcacg ctttcgcgct tttgctctct atctcgagaa
     2461 agattttcca atctttcgat cgcatcaaag aaaccggatc gttcgaccag aaaggactcc
     2521 aggggtttga tctgaaagga aagacgattg gcgtggtcgg cgtcggccgt atcggccagt
     2581 acgcgattcg catcgccaag ggattcggaa tggatgtcat cgcttacgac gctttcccga
     2641 gagccggttt ggaggcggaa ctcgggttca gatacgtcag tttcgaggag cttctgaaga
     2701 a
//
LOCUS       DAOPQT010000074         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_42202_length_2642_cov_3.163896, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000074 DAOPQT010000000
VERSION     DAOPQT010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42202_length_2642_cov_3.163896"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PKG74_02280"
     CDS             complement(<1..472)
                     /locus_tag="PKG74_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOB90137.1"
                     /translation="MFDFLFFRPLLNILVLISEYLVHGDFGFSVIALTVLVRILLFPV
                     FHKFLKSQQIMNKIQAEQKKIEAQYPKDKEKQLQAIMELYKANHINPFASIFLTFIQI
                     PVILALYKVIVAGLNGAATQSLYSFVRPITAVNYVFLGLINLKESNTIMLGLALG"
     gene            complement(465..788)
                     /locus_tag="PKG74_02285"
     CDS             complement(465..788)
                     /locus_tag="PKG74_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOB90138.1"
                     /translation="MIARKHRFNVLSSKGFEVFYRGKNLLIKTRPNDLTFPRFGVFLT
                     KKNIPLSSQRIALKRLIFDFIDKKRVLSSSLNQDFLIINLAKMDKIRDNKPIIEKELE
                     KAFNV"
     gene            complement(788..922)
                     /gene="rpmH"
                     /locus_tag="PKG74_02290"
     CDS             complement(788..922)
                     /gene="rpmH"
                     /locus_tag="PKG74_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOB90139.1"
                     /translation="MSVTYQPKKKKRSRTHGFLKRQSTPGGRRTIARRRAKGRRKLTV
                     "
     gene            1000..2376
                     /gene="dnaA"
                     /locus_tag="PKG74_02295"
     CDS             1000..2376
                     /gene="dnaA"
                     /locus_tag="PKG74_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOB90140.1"
                     /translation="MPKIKAMNKEELWKNVLSELEIQISKPNFLTWLKNSRMNEYENG
                     KATVVLPNHFAKEWVETKYHKLILGVLRSKDGSIKNITYSVDSVSFKKPTDTAPVAPP
                     TKQLVFSELKIDPETGLNPKYSLKSFVVGSSNELAYAASLAVIEEIGKKYNPLFIYGG
                     TGLGKFFFKQETAYEIKQNYKNINVKYVSSEKFTNDVIWAIRNKRIEDIKEAYRNVDV
                     LIIDDIQFIGGKEKTEEEFFHTFNALYEANKQIIISSDRPPRAIPILEERLRSRFEGG
                     MIADISFPDYETRMAIIKVKLQERNEVFDDPIVSIVAKKIQRNIRELEGILNKLIFYR
                     NQHREGFSAEQVESIVNDILTQSAVNVSPTQVIKAVSTFYEISPNDLIGRGRKKEIVE
                     PRQVAIYLLREILNMSYPYIAEKMGKRDHTTAIYAYEKINLSLSKDQGLNQKISLIKD
                     LINKPQNL"
BASE COUNT          821 a          620 c          541 g          660 t
ORIGIN      
        1 ctccgagcgc caaaccaagc attatcgtat tgctttcttt gaggtttatc aagcctaaaa
       61 acacgtaatt aaccgctgtt atcggcctca caaaagaata aagactttgg gtcgcggcgc
      121 cgttcaagcc ggcgacaatc accttgtaaa gggcgagaat gaccggtatc tggataaaag
      181 taagaaaaat actcgcgaaa ggattgatgt ggttggcttt gtaaagctcc atgatagcct
      241 ggagttgttt ttccttgtct ttgggatact gggcctcgat tttcttctgt tcggcctgga
      301 ttttgttcat tatttgctgg cttttcaaaa acttgtggaa aaccggaaaa agcagaattc
      361 tcaccaaaac cgtgagtgcg atcacggaaa agccaaagtc tccgtgaacc aaatactcgg
      421 aaatcaaaac caaaatattc aaaagcggcc taaaaaacaa aaaatcaaac attaaaggct
      481 ttttctaatt ctttttcgat aatcggctta ttatctctta ttttgtccat tttggcgaga
      541 ttgatgatca agaaatcttg gttaagagaa gacgataaaa cccttttttt gtcaataaaa
      601 tcaaaaatca gcctttttaa ggcgattctt tggctggata agggaatatt tttcttggtc
      661 agaaagacac cgaacctcgg gaatgtcaag tcattgggtc ttgttttgat caaaaggttc
      721 tttccccgat aaaaaacctc aaaacccttg cttgacaaga cattgaagcg atgctttctg
      781 gcgatcatca gaccgtcagt tttctccgtc ctttggcgcg acgcctcgcg atcgttcgtc
      841 tgccgcccgg agtgctctga cgtttgagaa agccgtgcgt gcggcttctt ttctttttct
      901 tcggctgata agttacggac atgtattaaa aataatcgaa ttatccacag gtagtcaaca
      961 ggttgtgttt tcttctttgt tttgactatc aagcttccct tgcctaaaat taaagccatg
     1021 aacaaggaag agctttggaa aaacgttcta tcggaattgg aaatacagat ttcaaagcct
     1081 aacttcctta cttggctgaa gaacagccgc atgaacgaat acgaaaacgg caaagcgacc
     1141 gttgttcttc cgaatcactt tgccaaagag tgggtggaaa caaaatacca caagctgatc
     1201 ctcggagttt tgagaagcaa ggacggatcg atcaaaaaca tcacctacag cgtcgacagc
     1261 gtttctttca aaaaaccaac ggatacggcc ccggtcgctc ccccgaccaa acagcttgtt
     1321 ttttccgagc ttaaaatcga tccggagacc ggtttgaatc ccaaatacag tctgaaatct
     1381 tttgtcgtcg gctcttcgaa cgaactggct tacgccgcct ccttggccgt gatcgaagaa
     1441 atcggcaaaa aatacaatcc tttgttcatt tacggcggca ccgggctcgg caaatttttt
     1501 ttcaagcagg agacggcata cgagatcaag cagaactaca agaacatcaa cgtcaaatac
     1561 gtttcctcgg agaagttcac gaacgatgtc atctgggcca tcaggaacaa aaggatcgaa
     1621 gacatcaaag aagcttaccg gaacgtcgat gttttgatta tcgacgacat ccaattcatc
     1681 ggtggcaagg aaaaaaccga agaagagttt ttccacacct tcaacgccct ttacgaagcg
     1741 aacaaacaga tcatcatctc ctccgatagg cctccgcgcg ccattcccat actcgaagaa
     1801 agactccgct cgagattcga aggcggaatg atcgccgaca tcagcttccc cgactacgaa
     1861 acccgcatgg ccatcatcaa ggtaaagctc caggaaagga acgaggtttt cgacgacccg
     1921 atcgtctcta tcgtcgccaa aaaaatacaa aggaacatcc gggagctcga aggcatcctg
     1981 aataagctta ttttctacag aaatcagcac cgggagggct tttccgccga acaggtggaa
     2041 agtatcgtga acgacattct aacccagtca gcggtcaatg tgagcccaac ccaggtgatc
     2101 aaggccgttt ccacttttta cgagatctct ccgaacgatt tgatcggccg gggacgcaaa
     2161 aaagagattg tcgaaccgag acaggtggcc atttatctgc ttcgggagat cttaaacatg
     2221 tcttatccct atatcgccga gaaaatgggc aaacgcgacc acacaaccgc catctacgcc
     2281 tacgagaaaa taaacctttc cctttccaaa gaccagggat tgaaccaaaa gatttcccta
     2341 attaaagacc tgatcaacaa accgcaaaac ctgtgaatta ctatgggtat aaacaatgga
     2401 taaaaataaa acattcccga tgacagcttt ttatccacaa ttgaattgtt aaaaaaaatc
     2461 gtttaaaaac gcgtcgaaaa caatttatcc accaatccac aacaataact acaacaagtt
     2521 ttatatatga aaataatcgt attacgtaac aatcttaaaa acgctttgga cgccgcctcg
     2581 agaacagtgg aggaaaacct gaacctgccg atcctgaaaa acatcctgat caaagcggat
     2641 ca
//
LOCUS       DAOPQT010000075         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_42536_length_2627_cov_3.487947, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000075 DAOPQT010000000
VERSION     DAOPQT010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42536_length_2627_cov_3.487947"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PKG74_02300"
     CDS             <1..566
                     /locus_tag="PKG74_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOB90141.1"
                     /translation="GFKFSTYATWWIRQAITRALADHSRTIRIPVHMVETISKYTQAR
                     RKLMQELGREPLAEEVAIEMGVNVEKIRHIQKISQEVISLESPVGEEEDSTLSEFIED
                     EKTLSPSQVVSRELLKDQIREVLNDLTERERKILELRFGLRDSTPHTLEEVGKIFGVT
                     RERIRQIEAKALEKIRQHQKSVKFGEY"
     gene            580..2430
                     /locus_tag="PKG74_02305"
     CDS             580..2430
                     /locus_tag="PKG74_02305"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOB90142.1"
                     /translation="MIQANEIRKKYLEFFKARGHAVIPSASLIPENDSTLLFTNSGMF
                     PLVPYLAGERHPAGNRLVNSQKCFRAEDIESVGDSRHDTFFEMLGNWSLGDYFKKEQL
                     EWWYEFLIEELKLDPKKLYQTVYAGDPAIGLGKDDEVVGIVKGIFKKYGIEAEEGPAT
                     TEKGEEGPGQEIDFDQERIFAYRDKNWWQRGDALGELGGPDSETFYDTGKPHDPKFGK
                     HCHLNCDCGRFLEIGNSVFMQFKKTAEDWEPLQNKNVDFGGGLERIAMVANNLQNIFE
                     SELFRPIIEKIEDLSGKKYDEDRQAFEIVSDHLKAAVFIMSEPAGIGPSNKDQGYIVR
                     RLIRRAAVYGRELGISDPVWIRQIGDIVRRIYKGIYPELEENKDFVDAELEKEEQKFT
                     KTLEKGLKEFSKIDQSDGVSGEEAFDLYQTFGFPIEIIEELAKEKSTSVDKEGFERKM
                     KEHQELSRTASAGMFKGGLADQSEETTKLHTAAHLLLAGLRQVLGEHVIQKGSNITAE
                     RLRFDFSHSEKLTDEEIKKVEDFVNEAILADKPVVCEEMDYEEAKKSGAMGVFDDKYG
                     NKVKVYDISPYSREICGGPHVTHTGILGKFKIQKEESVAAGIRRIRAVIL"
     gene            2438..>2627
                     /locus_tag="PKG74_02310"
     CDS             2438..>2627
                     /locus_tag="PKG74_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90143.1"
                     /translation="METEFLEIADGKYIVIAPASQSLTVYTRIIEERDRPREEIDEAE
                     LSNIVSQAIWKVFDRERTP"
BASE COUNT          758 a          606 c          717 g          546 t
ORIGIN      
        1 agggattcaa gttttcgact tacgccactt ggtggatcag gcaggcgatc acccgcgcct
       61 tggccgatca ttcgcgcacc atcaggatcc cggtccatat ggtggaaacg atctctaaat
      121 acacccaggc gcgccggaag ctgatgcagg agcttggacg cgaacccttg gccgaggaag
      181 tggccatcga aatgggcgtg aacgtggaga agatccgcca catccagaaa atttcccagg
      241 aagtgatctc tttggagtcg ccggtcggcg aagaggagga ctcgacgctt tcggaattca
      301 tcgaagacga aaaaacgctt tctccctcgc aggtggtttc gcgggagctt ctgaaagacc
      361 agattagaga agttttaaac gaccttacag aaagggagcg aaaaatactg gagttgagat
      421 tcggactccg cgacagcacg ccgcatacct tggaagaagt cggaaagatt ttcggcgtca
      481 ctcgggaacg catcagacag atcgaggcca aagctttgga aaaaatcagg cagcaccaga
      541 aatccgtgaa attcggagaa tactagacaa tccttctcga tgatacaagc caacgagatt
      601 cggaaaaaat atctggagtt tttcaaggcc cgggggcacg cggtcattcc ttcggcttcc
      661 ttgattccgg aaaacgattc cactttgctt ttcacgaact cgggtatgtt cccactcgtg
      721 ccttatctcg cgggcgagcg ccatccggcg gggaatagac tcgtcaattc ccagaaatgc
      781 ttccgggccg aagacatcga atccgtcgga gacagccggc acgacacttt tttcgaaatg
      841 ctcggcaatt ggtctttggg agattatttc aaaaaagagc aactcgagtg gtggtatgag
      901 tttttgatcg aagagcttaa actcgatccg aaaaaacttt accagacggt ttacgccgga
      961 gatcccgcaa tcggacttgg aaaagacgac gaggtcgtcg gaatagtcaa aggtattttc
     1021 aagaaatacg gtattgaagc cgaagaaggt ccggcgacaa ctgaaaaagg agaagagggc
     1081 ccgggacaag aaatcgattt cgatcaagag aggattttcg cttaccgcga caagaactgg
     1141 tggcagaggg gagatgcctt gggcgaattg ggcggaccgg actcggaaac tttctacgat
     1201 accggcaagc cgcacgatcc gaaattcgga aaacattgcc atctgaactg cgattgcggc
     1261 cgattcctcg aaatcggcaa ttcggttttc atgcagttca agaaaaccgc cgaagattgg
     1321 gaaccgcttc aaaacaaaaa cgtcgatttc ggcggcggac tcgagagaat agccatggtg
     1381 gcgaacaatc ttcaaaatat tttcgaatcc gagcttttcc ggccgattat cgagaaaatc
     1441 gaagatttgt ccggaaaaaa atatgatgaa gacagacagg ctttcgagat tgtaagcgat
     1501 catctgaaag cggccgtatt catcatgtcc gagccggccg gtatcggccc ttcgaacaag
     1561 gatcagggtt atattgtccg gcgtttgatc aggcgggccg cggtttacgg ccgcgagctc
     1621 ggcatatccg atccggtctg gataagacaa atcggcgaca ttgtccggag gatttacaag
     1681 ggtatttatc ccgaactcga ggaaaacaaa gattttgtcg acgccgaact cgaaaaagaa
     1741 gaacaaaaat tcacgaaaac tttggaaaaa ggattgaagg agttttccaa aatcgatcaa
     1801 tcggacggcg tctccggaga agaagcattc gatctttatc agacgttcgg ttttccgata
     1861 gagatcatcg aagagctggc caaggaaaaa agtacctccg tcgataaaga aggattcgaa
     1921 agaaaaatga aagaacacca agagctttcc cgcaccgctt cggccggaat gttcaagggc
     1981 ggcttggctg accagagcga agaaacgacc aagctccaca cggccgccca tttgctcttg
     2041 gccggactgc ggcaagtatt gggcgaacac gttatccaga aaggaagcaa catcaccgcc
     2101 gagagacttc gtttcgactt ttcccattcg gagaaattga ccgacgagga aatcaaaaaa
     2161 gtggaggatt ttgtgaatga ggccattttg gcggacaaac cggtggtttg cgaagaaatg
     2221 gattacgaag aagcgaaaaa atccggggcc atgggggttt tcgacgataa atacgggaat
     2281 aaggtgaaag tttacgatat ttctccctac agtcgcgaga tctgcggcgg tccgcacgtg
     2341 actcacaccg gaatcttggg aaaattcaag attcaaaaag aagaatccgt ggccgcggga
     2401 attagacgca tcagagcggt tattttataa ttcagtcgtg gagactgaat tcttggagat
     2461 agccgacggg aagtacatcg tcatcgcgcc cgcttcacag agcctcacgg tctacacgag
     2521 gatcatcgag gaacgcgatc ggccgcgcga agaaatcgac gaagccgaat tgtcaaacat
     2581 agtctcccag gcgatttgga aggtgttcga ccgggagcgg actccgg
//
LOCUS       DAOPQT010000076         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_42666_length_2621_cov_3.069758, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000076 DAOPQT010000000
VERSION     DAOPQT010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42666_length_2621_cov_3.069758"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /locus_tag="PKG74_02315"
     CDS             complement(<1..1498)
                     /locus_tag="PKG74_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOB90144.1"
                     /translation="MNHKKIFLAATFLAVFGLFGFNPPKTEAAMTAAEIQTLMQSLQQ
                     QISQLQQQLSSLRQTPTQTTATATGWCYNFSTNFGVGTISQDVLALETALQKEGLLSY
                     PDNVFNESTASAVTRLQEKYRADVLSPVGLTRGTGFVGSSTRAKLNALYGCGISSAST
                     SIPYITVIYPNGGETFTTGQTYNITWRSNGINKVIVTECADTPRLNPPYTCEPLSGYG
                     ETDAGLGYRSWYVDPNNPFYAGVSRIRIRVSDANNPNIYDESDNYVTVLDPYEPTYQN
                     SYDSSSSNTSSSAPGQITNLTAQNSSGYALLNWDSPYNGGSLIQNYKVYRGTSSGNLS
                     LLATTQFTNYTDSSVARGVTYYYRVKAVNASGESGFSNEVSVLIPSSSSYSYYSDSSE
                     SVRIASVYPSSITQGQTFSVSWQVSSHFQGCRVWFQGTNETKPNLAISGNTSVSFSTL
                     GVPAGTYAVSLRCFRPSEIGAMSNYQNADYMTNSAYNSIGKSDTSYVNI"
     gene            1678..1821
                     /locus_tag="PKG74_02320"
     CDS             1678..1821
                     /locus_tag="PKG74_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015010594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HOB90145.1"
                     /translation="MMWLIIGIVSLAAWIAGFFVFKVAGLFIHLLLIVAVVSFLVRII
                     KGK"
     gene            1831..2166
                     /locus_tag="PKG74_02325"
     CDS             1831..2166
                     /locus_tag="PKG74_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90146.1"
                     /translation="MAKITKAKIKSSARKIRNATEEQFNEMEKNYEKLMGSVNNMSAP
                     GLLFVSFIGIAIILLLDSALFDLIGLLVLIYPFYLFVQKTGHHDGYCEGYYDHMTKDK
                     DTGRSDKKD"
     gene            2228..>2621
                     /locus_tag="PKG74_02330"
     CDS             2228..>2621
                     /locus_tag="PKG74_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOB90147.1"
                     /translation="MKKGFKNNIEKLTLENDNFRKVLYTAGHCQLVLMSLKPKEDIGV
                     EIHPDNDQFFRFEAGHGKAIIDGNEYDLEDGDAVIVPAGAEHNIMNVSETEGLKLYTI
                     YSPAHHKDGIVRLTKEEAMANEADFDGVT"
BASE COUNT          729 a          604 c          618 g          670 t
ORIGIN      
        1 gtatattgac atatgaagtg tccgatttgc cgatactatt gtacgcggaa ttagtcatat
       61 agtcggcatt ctgataatta gacatcgcac cgatctcgct cggtctaaaa catcgcaggc
      121 ttactgcata cgttccggcc gggacaccga gcgtggaaaa actaacgctg gtatttccgc
      181 ttatcgcaag attaggtttg gtctcgttcg tgccctggaa ccagacgcgg catccctgga
      241 aatgagacga aacttgccag cttaccgaga aagtttggcc ttgtgttatg gacgaaggat
      301 aaacactcgc aattctcacg gattcgctac tatccgaata ataagaatag gaagagctcg
      361 agggaatcaa aaccgaaact tcgttggaga aaccgctttc gccgctggcg ttcaccgcct
      421 tcacgcgata atagtaggtt acgcctcttg ccacactcga atcggtatag ttggtgaatt
      481 gggtggtggc gagtaaagaa agattacccg aagaagttcc ccgataaacc ttataattct
      541 ggatcaagct tccgccatta tagggagaat cccaatttaa aagagcgtag ccggagctgt
      601 tttgggcggt caaattggtg atttggccgg gagcggaact cgaagtattg gaactgctcg
      661 agtcataaga attttggtag gtcggttcgt acggatcgag aacagtcaca taattatcgc
      721 tttcatcgta gatgttggga ttattagcgt cggagactct tattcttatt cgacttactc
      781 cggcgtaaaa cggattattg ggatcaacat accatgaccg ataacccaat ccggcgtcgg
      841 tctcaccata accggatagt ggttcacaag tatacggcgg atttaaacgt ggcgtatcgg
      901 cgcattcggt cactattact ttattgattc cattcgatct ccaggtaatg ttgtaagtct
      961 ggccggtggt aaaagtctct cccccattcg gatagataac agtaatataa ggaatcgatg
     1021 tcgacgcgga cgaaattccg catccgtaga gcgcgtttaa tttggctctg gtcgagctac
     1081 ccacgaaacc ggttccccgc gtaagtccta ccggagaaag gacatccgct ctgtattttt
     1141 cctggagcct cgtgacagcc gaagcggttg actcgttgaa cacgttatcg ggatagctca
     1201 aaagcccttc tttctgtaag gcggtttcga gcgccagaac atcctggcta atagtcccga
     1261 ctccgaaatt ggtgctgaag ttgtaacacc aaccggtcgc ggttgccgtc gtctgcgttg
     1321 gtgtttggcg caaagacgac aattgctgtt ggagttggct gatctgttgc tgcaacgact
     1381 gcattaatgt ttgtatttcc gcagctgtca tggccgcttc agtttttggc gggttaaaac
     1441 caaaaagacc gaaaacagcc aagaaggttg ctgctaagaa tatcttttta tgattcatgt
     1501 ttttttatat cgactttaaa ttaaagtgat ccgacccttt gtttatatga taacataact
     1561 tccccagagg ctcccgaaaa aaagacgtgg aacgccggtt tgaaaaaatg tgaaagtcag
     1621 cggcggcaat tgtgctataa tcaaataaag cgtcgataca ttaaaaataa ataaatcatc
     1681 atgtggttaa tcataggcat tgtctcgctc gcggcctgga tcgccggctt ttttgtattt
     1741 aaggtcgccg ggctctttat ccacctgctt ctcatcgtgg cggtggtttc tttccttgtc
     1801 cgcataatca aaggaaaata aataattatc atggcaaaaa taaccaaagc aaaaatcaaa
     1861 agcagcgccc gcaaaattcg taacgcgacc gaagaacaat tcaacgagat ggagaaaaat
     1921 tacgagaagc tcatgggaag cgtcaacaac atgagcgctc cagggctttt gttcgtttct
     1981 ttcatcggaa tagccatcat cctcttgctc gacagcgccc tattcgatct tatcggatta
     2041 ctggtattga tttatccgtt ctaccttttc gtgcagaaaa ccggacatca tgacggttat
     2101 tgcgaaggat attacgatca catgaccaag gataaagaca ccggcaggag cgacaaaaaa
     2161 gactaaactc gacatcgttc tgacttgggg attttttcct ttacctgttc tgtattacaa
     2221 ttacaccatg aaaaaaggtt tcaaaaacaa tatcgagaaa ttaacccttg agaacgacaa
     2281 tttccgaaag gtcctctaca ccgccggaca ctgccagctc gttttgatga gcctcaagcc
     2341 caaagaagac atcggcgtcg agatccatcc ggacaacgat caattcttca ggttcgaagc
     2401 cggtcacggc aaagcgatca tcgacggtaa cgaatacgac ctcgaggacg gcgacgcggt
     2461 cattgttccg gccggagccg aacataacat catgaacgtt tccgaaaccg agggattgaa
     2521 actatacacc atctattccc cggcccatca caaagacgga atcgtgcggc tgaccaaaga
     2581 agaagcgatg gcgaacgaag cggatttcga cggcgtgaca a
//
LOCUS       DAOPQT010000077         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_42819_length_2615_cov_3.122656, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000077 DAOPQT010000000
VERSION     DAOPQT010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42819_length_2615_cov_3.122656"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..716
                     /locus_tag="PKG74_02335"
     CDS             270..716
                     /locus_tag="PKG74_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90148.1"
                     /translation="MKKIFLIAAIAILALGTMAFMAISTKAAAPTGTLTGTPITINIG
                     ERAALPTLTVTAPNPSDITDEHDIRIWAIHIGTQVIWDESVTTFTPGGTAAEKVSGSI
                     TYEKYLGSAENVVAMIEVISDFDPGDTLEINGLGLSLTVAAAWAKR"
     gene            701..1999
                     /locus_tag="PKG74_02340"
     CDS             701..1999
                     /locus_tag="PKG74_02340"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOB90149.1"
                     /translation="MGQALNWSVDGSSFRPADNSTRLDFTTAGDEDNIANVQVGVSNS
                     VKSQENVTYTVQFTLPGGSYIPKNGIFKITFPVGTILTNATFTTNLFNLGDFALNKNN
                     NILTVTRNGEGPAYIGSPTDITFTISGVTNTTVAAANKQITVENQTSGGGFLASGISA
                     SFAILEVPAVIMDLTCESSGQAGAVFLRFTEPDGAAGGAYIVKHSATAITEGDWNSGT
                     IVPQNWIGKAKGVARQELATGLTPNNRYYFAIKSAAAVGDYESDISNSISCIAPSSAP
                     SNIDNTAPTTRFVSPVPGSTIKTGTILLKGTARDTGGSSVRRIEISLDNGTTWSEATV
                     VGEDGADKLWEYAIQNAKARTITVLARSIDWTGNVETPTSITFTVSENGEDITVTPPS
                     VPVGASADEIKAAITTIQQQLVLLIQQLIQRLLAELRVML"
     gene            complement(2188..2550)
                     /locus_tag="PKG74_02345"
     CDS             complement(2188..2550)
                     /locus_tag="PKG74_02345"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOB90150.1"
                     /translation="MLQKFKIETIEFLNSQLKELNNQGIFWIEDLKCNFDVEIGRDML
                     VDYDKNGIDTFVLWSGDSDFAGPVSQLIKDGKKVILFATARRIASEFDSIKGLLIFDI
                     RKIRDFICWNREVSKKSA"
BASE COUNT          700 a          645 c          580 g          690 t
ORIGIN      
        1 cgaaacagaa ttcaaaaccc tcgcttagcg ggggttttga attaaataag cttttctagt
       61 aaaatgatta catcacgacg aaaggggatc cgaaaggtta attacctaag gagccccttt
      121 cttttttgct tttcaaccgt gaagttatca acaaggaaat ccgccgattt tatcgcataa
      181 tatattcata tagcgctcgt cccttttact cgcctatcag cgcttaatta atggcgctgg
      241 caccgtatcc gtaaaacgag acaaagaaaa tgaaaaagat cttcctaatc gccgcgatag
      301 ccattttggc tctcggaacc atggccttta tggcgatttc caccaaggcc gccgcgccta
      361 ccggaacctt aaccgggacg ccaataacca ttaatatcgg cgaaagggcg gcgctgccta
      421 ctcttaccgt taccgctccg aatccgagcg atatcaccga cgaacatgac ataagaattt
      481 gggctatcca tataggaacg caggttatct gggatgaaag cgttaccaca tttacgcccg
      541 gcggaaccgc cgcggaaaag gtttccggct ctattactta cgagaaatat ctaggcagcg
      601 ccgaaaacgt agtggctatg atcgaagtta tttccgattt tgacccggga gatactttgg
      661 aaattaatgg cttggggtta tcgcttacag tggcggcggc gtgggccaag cgctgaattg
      721 gtcggttgac ggctcgagtt ttaggcctgc cgacaatagc acgcggctgg atttcacgac
      781 tgccggcgat gaggacaata tagccaacgt tcaggtcgga gtcagtaatt ccgtaaaaag
      841 ccaagaaaac gttacctaca ccgtgcaatt tacccttccc ggtggaagtt acataccgaa
      901 aaacggtatt tttaagatca cttttcctgt tggcactatt ttgacgaacg ccacttttac
      961 gacaaatctc tttaacctgg gagattttgc gttaaacaag aataacaata tccttaccgt
     1021 tacgagaaac ggtgaaggcc ctgcctacat cggttctccg accgatatca cgttcactat
     1081 cagcggcgtt acaaacacaa ctgtcgccgc cgctaacaaa caaattaccg tagaaaatca
     1141 aaccagcgga ggaggttttc ttgcttccgg aatttccgcg tctttcgcta ttcttgaagt
     1201 tccggcggtt attatggatc ttacctgtga atcctccggt caagccggag ctgtcttcct
     1261 ccgcttcacc gaacctgacg gagccgcagg cggagcttac attgttaaac attccgccac
     1321 agctatcacc gaaggcgact ggaacagcgg gaccatcgtt ccccaaaatt ggatcggcaa
     1381 agccaaaggc gtggctcgtc aggaactcgc gaccggctta actccgaata atcgctatta
     1441 ctttgcgata aagtccgcgg ccgcggtcgg agattatgaa tcggatatct ccaattcgat
     1501 ttcttgtatc gctccttctt ctgctccatc gaatatcgac aataccgctc cgacaactcg
     1561 ctttgtctct ccggttcccg gatctactat caaaaccgga accatactct tgaaaggtac
     1621 ggcccgggat accggagggt cgtccgttcg gagaatagaa atctctttgg ataacggaac
     1681 aacttggagc gaagcgaccg ttgtcggcga agacggggcg gacaagcttt gggaatacgc
     1741 gatccaaaat gccaaggccc gaaccatcac tgttctggct cgctccatcg actggactgg
     1801 caatgtggaa accccgactt ccattacctt caccgtttcc gaaaacggag aagacataac
     1861 cgttactccg ccttcggttc cagtcggcgc ttcggcggac gagatcaagg cggccattac
     1921 gactatccag cagcagttgg tgcttttgat ccaacagctg attcagcggc ttttggccga
     1981 actacgggtg atgctctaga ctagataata cggatttaac gattgatgtt tatacatatt
     2041 gtatccctca ccatagatta cggaggaggg gctttgtcca caaaataagt ttttgacaca
     2101 agagtcgcga tttagtagga tataaatgat cggaaacgat cgctaactct agagcttggg
     2161 ggccctcatc gggtcccctt tgctgtatca ggccgatttc ttggaaactt ctctattcca
     2221 gcatataaaa tctcgtattt ttcgaatatc aaaaataagg agtcctttta tagaatcgaa
     2281 ctccgaagcg attcttctgg ccgttgcgaa gagaataacc tttttaccgt cttttataag
     2341 ctgacttacc ggaccggcaa aatcactatc tccgctccag agtacgaaag tatcgattcc
     2401 atttttatcg taatctacca acatatctcg ccctatttcg acatcaaagt tacactttaa
     2461 gtcttctatc cagaaaatac cttgattatt caattctttt aactgggaat tcagaaattc
     2521 gatagtttct attttaaatt tttgaagcaa tgattttctg atgaaatttt taataatttc
     2581 cggagattcg ggaggaatgc tggatacgtc tatcg
//
LOCUS       DAOPQT010000078         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_44119_length_2556_cov_2.620952, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000078 DAOPQT010000000
VERSION     DAOPQT010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44119_length_2556_cov_2.620952"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /gene="groEL"
                     /locus_tag="PKG74_02350"
     CDS             complement(<1..886)
                     /gene="groEL"
                     /locus_tag="PKG74_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOB90151.1"
                     /translation="MAKQILFDEEARKSLKKGIDKLARAVTMTLGPRGRAMVIEKGYG
                     APQVTFDGVTVAKEIELEDKYENLGADLLKQAADKTNDNVGDGTTTAVVLAHAMIEEG
                     ERQISDKGFNVIQLSEALKKGSDAVIKALEDQKELIDDDKKVEEVATLSAKNNEIGKL
                     IAEVMKKIGKEGVVTIEDSNTVSNSYEIVEGMQFDKGYISQYMISNPERMEAVLEDPY
                     ILVTDQKISAINDLLPLLEKLIKVGKKELVIVADDVDGEALATLVVNKLRGIFNVLAV
                     KAPGFGDRRKEMLQDIATV"
     gene            complement(917..1207)
                     /locus_tag="PKG74_02355"
     CDS             complement(917..1207)
                     /locus_tag="PKG74_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOB90152.1"
                     /translation="MNIKPLSNHIFLEPVEEEKATESGIIIPDTADKEKPVKGKVIAV
                     GSGKLNEKGERTPMSVKVGDTVLFKKYGPDEIEIDGKKYLVGDEDDVLAIVE"
     gene            complement(1294..2325)
                     /locus_tag="PKG74_02360"
     CDS             complement(1294..2325)
                     /locus_tag="PKG74_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOB90153.1"
                     /translation="MEIVFWAVIILFLSLLGVALFLLLRKSKPADNSLILLQNQINEM
                     SRVLDAKLGELPKVMQSQFGESSRMIKEITEELTKVGEGQKQVVSLADQLKDLQDILK
                     NPKQRGVLGEYFLEETLKNVLPPNSYEIQYKFKDGEMVDAAVFVKDKIIPVDSKFSLE
                     NYERILSENDSQRREELEKQFRDDLKNRIDETSKYVRPEENTMDFAFMFIPSEAIYYD
                     LLVNKVGAVKTNTRDLIEYAFKEKHVIIVSPTSFLAYLQTVLQGLKALQIEESAKEIK
                     KRVEELGKHLASYEGYMKKLGSHLGTSVGMYNQAYKEFGKIDKDVLRISGSTANIEPM
                     VLEKPEAEE"
     gene            complement(2372..>2556)
                     /locus_tag="PKG74_02365"
     CDS             complement(2372..>2556)
                     /locus_tag="PKG74_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90154.1"
                     /translation="VILRPERPVGSDYRIALSYAYDYFGCCDENCLSDCRFKESATDQ
                     SDADFCLINPFAAENP"
BASE COUNT          518 a          709 c          560 g          769 t
ORIGIN      
        1 taacggtggc gatatcctgg agcatttctt ttctcctgtc gccgaatccc ggcgccttca
       61 ccgccaagac gttaaagatc cctctcaatt tattgaccac gagcgtggcc aaagcttcgc
      121 cgtccacgtc gtcggcgacg atcactaatt cctttttgcc gactttgatc agtttttcca
      181 aaagcggcaa aagatcgttg atggccgaaa tcttctgatc ggtgacaagg atataggggt
      241 cttcgagtac cgcctccatt ctttccggat tggaaatcat gtactgcgag atatatcctt
      301 tatcgaactg cattccctcg acgatctcgt aggaattgct caccgtattc gaatcctcta
      361 tggtgacgac gccttccttg ccgattttct tcatcacttc ggcgatcaac tttccgattt
      421 cattattttt cgccgacagc gtggccactt cttccacttt cttgtcgtcg tcgatcaatt
      481 ctttctgatc ttccagagcc ttgatcaccg catcgcttcc ttttttcaaa gcttcggaaa
      541 gctggatgac attgaatcct ttgtcgctta tctgacgctc gccttcctcg atcatggcgt
      601 gggccaagac cacggccgtg gtcgtgccgt ccccgacatt atcgttcgtc ttgtcggccg
      661 cctgtttcag caaatcggct ccgagattct cgtatttgtc ttcgagctcg atttccttgg
      721 cgacggtgac gccgtcaaac gtcacctgcg gggcaccgta tcctttttcg atgaccatcg
      781 cccgtccgcg cggacccaaa gtcatggtga cggctcgcgc caatttgtct attccttttt
      841 tcaaggattt tctcgcttct tcgtcgaata aaatttgttt agccatggtg ttgatttttt
      901 ttaaacgcga tccgacctat tccacgatcg ccagaacgtc gtcttcgtct ccgaccaaat
      961 attttttccc gtcgatttcg atttcgtcgg gaccgtattt tttgaataga accgtgtcgc
     1021 cgaccttgac cgacatcggc gtccgttcgc ctttttcgtt caattttccc gatcccacgg
     1081 cgataacttt gcctttgacc ggtttttctt tgtcggccgt atccgggatg atgatgcccg
     1141 actccgtggc cttttcttct tcgaccggct caaggaaaat atggtttgat agaggtttta
     1201 tgttcatttc gacttatatt atatcatatc ttgccgcggt tttgtcaagc cgttttaaga
     1261 cgacctccga ttgcttgacg gctttcccga aggtcattcc tcggcttcgg gcttttccaa
     1321 gaccatcggc tcgatatttg ccgtcgaacc gctgatccgc aaaacgtctt tgtctatttt
     1381 cccgaattcc ttgtaggcct ggttgtacat tccgaccgag gtcccgagat ggcttcccag
     1441 ctttttcatg tatccttcgt agctcgccag atgcttgccg agctcctcca cccgtttttt
     1501 gatctccttg gccgattcct ctatctgcag agctttgaga ccctggagca cggtttggag
     1561 ataggcgaga aagctagtcg gagaaacgat gatcacgtgt ttttccttga aagcgtattc
     1621 gatcaaatcc ctcgtgttcg ttttaaccgc acccacttta ttcaccagga gatcgtaata
     1681 aatcgcctcc gaaggaatga acatgaaagc gaaatccatg gtgttctcct ccggacgcac
     1741 gtatttcgag gtttcgtcga tgcggttttt cagatcgtcc ctgaattgtt tttccaactc
     1801 ctcccttctc tgggaatcgt tttctgacaa tatcctttcg tagttctcca aggagaactt
     1861 ggaatccacc ggaatgatct tgtccttcac gaaaaccgcg gcgtccacca tttcgccgtc
     1921 tttgaacttg tactgtattt cgtaggaatt cggcggcagg acgttcttaa gggtctcttc
     1981 caaaaaatac tctccgagca ctcccctttg cttcgggttt ttgaggatat cctggagatc
     2041 tttcagctga tcggccaaac tcaccacctg tttctggcct tcgcccacct tggtcagctc
     2101 ctcggtgatc tctttgatca tccggctgct ttcgccgaac tgcgactgca tcacctttgg
     2161 cagttctccg agtttcgcgt ccaaaacccg gctcatttcg ttgatctggt tttgaagcaa
     2221 tatcaaagaa ttgtcggccg gtttcgactt tctcaagagc aaaaacaaag caacgcccaa
     2281 aagcgacaag aacaaaatta tgaccgccca gaaaactatt tccatgggtt ctattttaga
     2341 ccaaaaaaag ccggctcgcc acccgatggt ttcaggggtt ttcggcggca aaagggttga
     2401 tcaggcaaaa atccgcgtcg ctctgatccg ttgccgattc cttgaaccgg caatcgctaa
     2461 ggcaattttc gtcgcagcag ccaaaataat cgtaggcgta gctcagagcg attctgtaat
     2521 ccgatccgac cgggcgctcc ggtcgcagga tcacgg
//
LOCUS       DAOPQT010000079         2522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_44931_length_2522_cov_3.463316, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000079 DAOPQT010000000
VERSION     DAOPQT010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44931_length_2522_cov_3.463316"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKG74_02370"
     CDS             <1..240
                     /locus_tag="PKG74_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOB90155.1"
                     /translation="DTFPLMKINEGSAEIEHEATVSKINEEKLFYLTSRGLTEDEAMS
                     LIVSGFIEPIVKELPMEYAIELNRLIELEMEGSVG"
     gene            242..838
                     /locus_tag="PKG74_02375"
     CDS             242..838
                     /locus_tag="PKG74_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOB90156.1"
                     /translation="MKTILLDIKKNQKISVAEDTQFVLADSRGFEEEFTLNLVFQKPG
                     VASEVLGIFNLPAAEKIHLTTSSNHLVPGTSCHTYIKAVLNEKSDFDYRGEIFIGKKA
                     RQTTAYLHDHVLVAGDNIKRNSQPILQIETNDVQASHGSTTGRIDRNELYYLESRGLE
                     KNKAEETIIQGFLIDLIKKIKDENVKRSVLDKLGLKNE"
     gene            835..2049
                     /locus_tag="PKG74_02380"
     CDS             835..2049
                     /locus_tag="PKG74_02380"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOB90157.1"
                     /translation="MKDIRKDFPILERKINGRELVYLDNAATSQKPKQVLEAIAGYYS
                     NHNANIHRGLHTLSEEATDMYEKSREKIAGFIGANCPEEVVLTKGATESLNMVAAMWG
                     ATNLKKNDTILFGDFEHHSNMVPWLEAAEKTGAKIEYLESDENGEIRMEEVEKKMTDK
                     VKLIAITHASNVLGTIVPIKEICRLAAKKGILVCVDGAQAAPHLKVNMADLGCDFYCF
                     SAHKMLGPMGVGVLWARKEILEKLKPYEFGGGMIDEVSYRKATYAAIPARFEAGTPNV
                     AGAIGFAAAVDYLEKIGMDNIRKHEIALSRRLLEELRQIEGLKVIGPKEPEKRSGLVS
                     FAIAGIHAHDIASVLNDYGIAVRAGHHCVMPYHAKIGLSGTTRASFYLYNTEEEIDRL
                     VEGIKKAVEILS"
     gene            2051..2434
                     /locus_tag="PKG74_02385"
     CDS             2051..2434
                     /locus_tag="PKG74_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOB90158.1"
                     /translation="MEDFYREEFMDIFRHPHNRGTLENPSVFAYKKNPMCGDSLVLRL
                     KIKDGIIEDAKFEGEACAVSIISSSILTDYLIGKSVDEAKKINKDRLLEMISLNLTTS
                     RISCAALVLYALEEALKNYENKKSE"
BASE COUNT          768 a          548 c          652 g          554 t
ORIGIN      
        1 gacactttcc ccttgatgaa aatcaacgag ggatcggccg agatagagca cgaagcgacc
       61 gtgagcaaaa tcaatgaaga aaaactattc tatttaacga gccgggggct taccgaagac
      121 gaggcgatga gcttgatcgt ttccggattc atcgaaccca ttgtcaaaga attgccgatg
      181 gaatacgcca tagaattgaa taggctgatc gagctcgaaa tggaaggaag tgtcggctaa
      241 catgaaaacg atccttctcg atatcaaaaa aaatcaaaag ataagtgtcg ctgaagacac
      301 gcaatttgtt ttggcggaca gtcgcggatt cgaagaagaa tttactttga atcttgtctt
      361 ccaaaagccc ggggtggcct ccgaggtgtt ggggattttc aatctgccgg cggcagagaa
      421 aatccattta accacttcct cgaatcatct ggttccagga acttcgtgcc atacatatat
      481 caaggccgtt ttgaacgaaa aatcggattt cgattaccgt ggagaaatct tcattggaaa
      541 aaaagcccgg caaacaaccg cctatttgca cgatcatgtt ttggtcgccg gagacaatat
      601 caagagaaac tcccagccga tccttcaaat cgaaacgaac gacgtccaag cgtcgcacgg
      661 atccacgacc ggaagaatag atcgaaacga gctttattat ttggaaagcc gggggctcga
      721 aaaaaacaaa gccgaagaaa cgatcattca aggattcttg attgacctca taaaaaagat
      781 caaagacgag aatgtcaaaa ggtcggtgct ggacaaattg ggattgaaaa acgaatgaaa
      841 gacataagaa aagattttcc gattttggaa agaaaaatca acggccgtga attggtctat
      901 ttggacaacg ccgcgacttc gcagaagccg aagcaggttt tggaggcgat cgccgggtat
      961 tattcgaatc acaacgccaa catccaccgg ggattgcata ctttaagcga agaggcgacc
     1021 gatatgtacg agaaatccag agaaaagatc gccggattca tcggcgcgaa ctgtccggaa
     1081 gaagttgttc tcaccaaagg agccaccgaa tctttgaata tggttgcggc aatgtggggc
     1141 gccaccaatt tgaaaaaaaa cgacacaatc cttttcgggg atttcgaaca ccacagcaat
     1201 atggttccct ggctggaagc ggctgaaaaa accggagcga agatcgaata tctggaatct
     1261 gacgaaaacg gcgaaatccg tatggaagaa gtcgagaaaa aaatgaccga caaggtgaaa
     1321 ttgattgcta tcacccatgc ttcgaatgtt ttggggacca ttgttccgat caaagaaatc
     1381 tgccggctgg cggccaaaaa aggaatcttg gtttgcgttg acggcgcgca agcggcgccg
     1441 caccttaaag tgaatatggc tgacctcggg tgcgattttt attgtttttc ggcgcacaaa
     1501 atgctcggac ccatgggtgt gggcgttctc tgggcgagaa aagagatttt ggaaaaactc
     1561 aaaccttatg aattcggagg cggtatgatc gacgaggttt cttaccgaaa ggccacttat
     1621 gcggctattc cggcacggtt cgaagccggt acgccgaatg tcgccggagc aatcgggttt
     1681 gccgcggccg tggattatct cgaaaaaata ggaatggata acatcagaaa gcatgaaatc
     1741 gcgttgagca ggcgtttact tgaagagttg aggcagatcg agggactgaa agtgatcggc
     1801 ccgaaagaac cggaaaaaag aagcggcctc gtttctttcg cgatcgccgg aatccacgcg
     1861 cacgatatcg cttccgtatt gaacgattac ggaatcgccg tcagggccgg gcaccattgc
     1921 gtcatgccgt atcacgcgaa aatcggcctt tcgggaacaa ccagggcgag cttttatctt
     1981 tacaataccg aagaagagat cgatcggctg gtcgagggaa taaaaaaggc tgtggagatt
     2041 ttgagttgag atggaggatt tttaccgcga agagttcatg gatattttcc gacatcccca
     2101 taacagggga accttggaaa acccgtcggt ctttgcctat aaaaaaaatc ccatgtgcgg
     2161 agactccttg gttctgcggc tgaagatcaa ggatggaatt atcgaagacg ccaaattcga
     2221 aggcgaagcc tgcgcggtca gcatcatctc gtcgtcgatc ctcacggact atttgatcgg
     2281 gaaaagcgtc gacgaagcaa agaagataaa caaagaccga ttgctcgaaa tgatcagctt
     2341 gaatttgacc accagccgta tttcctgcgc cgcattggtt ctttacgctt tggaagaggc
     2401 gttgaaaaac tatgaaaaca aaaaatccga ataaaatcac caaagattcg aaccttgccc
     2461 aagtggtgta cgaacatccg gaagccgtgg aggtattgct ggattacggt ctccattgca
     2521 tc
//
LOCUS       DAOPQT010000080         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_45038_length_2517_cov_3.353371, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000080 DAOPQT010000000
VERSION     DAOPQT010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45038_length_2517_cov_3.353371"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..224)
                     /locus_tag="PKG74_02390"
     tRNA            complement(151..224)
                     /locus_tag="PKG74_02390"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Val,seq:cac)
     gene            complement(298..657)
                     /gene="rplL"
                     /locus_tag="PKG74_02395"
     CDS             complement(298..657)
                     /gene="rplL"
                     /locus_tag="PKG74_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOB90159.1"
                     /translation="MEKFQNIISEIEKLNALELSELVKALEEKFGVSASAPAAAAASA
                     AVAEEKTSFNVLLKASGDQKINVIKVVREVTGLGLKEAKDLVDAAPKVVKEGLKKEEA
                     DELKKKLEEVGATAELQ"
     gene            complement(668..1147)
                     /locus_tag="PKG74_02400"
     CDS             complement(668..1147)
                     /locus_tag="PKG74_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOB90160.1"
                     /translation="MLTRKEKQEILRQGTEDVKSSQSMVFLDFTGVPTGKLNAFRSEM
                     RKLGGHFDVVKKRLLKLIFSRANVAIDPETFEGQVGTVFSPTDVFAVSGPIYKAEGFK
                     VLGGFDIAAGKAVPATDIIAMGKLPSREILLGQVVGTIAAPLTSLLYVLKERSKQTA"
     gene            complement(1297..1995)
                     /locus_tag="PKG74_02405"
     CDS             complement(1297..1995)
                     /locus_tag="PKG74_02405"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOB90161.1"
                     /translation="MAEKINIFEENNPENEDDSGFVNESYEELIRHLETDGYIQTPVI
                     AEAFRKIDRKDFVPPELGEEAYFDSALAIGFDQTISQPLVVAFMIELLRPKPGERVLD
                     VGSGSGWQAAILADLVGETGQVVGIERIKELAEKAKENIGKYGFIDSGRVKIVVGDGS
                     RKMEGEPPFNKIIAAAAAEEIPEAWKEELVVGGRIVAPVGQSIVVIDKIKPDQFETKE
                     YFGFSFVPLVKSKN"
     gene            2067..>2517
                     /locus_tag="PKG74_02410"
     CDS             2067..>2517
                     /locus_tag="PKG74_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90162.1"
                     /translation="MDLETSAVEEQASVPAFRVKVTGTETVVIPVPNEDTKDFRGVVE
                     AIKAKVAEAVYRKPPRIVVAVTAIEISRAVADHLKSQRPFGRKQITEGLVVPQSLTLV
                     VNDAPVFRTSKPLSCERHAFMTVVERMSGVKLKELLCSGSSFVIYNQV"
BASE COUNT          569 a          654 c          627 g          667 t
ORIGIN      
        1 gacctgggag acaaattcgt cgttctttcg gttgccgatc tcttggcgaa cgcgatctat
       61 cgccttcaca acgatcggtc gatctccgaa ttgatcctct agcaacatga gcaggccaac
      121 cggcctgctt ttcttatatt tgtcaaaatg tgggcactac tggattcgaa ccagtgacct
      181 tacgaatgtg aatcgtacgc tctaaccaac tgagctaagc gcccgaacgg tcgatataaa
      241 cataacaaaa accggctttt ttgaacagcc ggttttctaa tcgaagattc cttggaatta
      301 ctgcaattcc gcggtggcgc cgacttcttc caatttcttt ttgagttcgt cggcttcttc
      361 tttttttaat ccttccttga cgactttcgg ggcggcgtcg accaaatcct tggcttcttt
      421 gaggccaagg ccggtcacct ctctcaccac cttgatgacg ttgatcttct ggtcgcccga
      481 agccttgaga agaacgttga aagatgtttt ctcttcggct acggcggccg aagcggccgc
      541 ggccgccgga gcgctggcgg aaacgccgaa tttttcctca agagccttca ccaattccga
      601 aagctccaag gcgttgagtt tctcgatttc cgagatgatg ttttggaatt tttccatgtt
      661 ggtttgttta tgctgtttgt ttcgacctct ctttcaagac gtaaagaagc gaagtgagcg
      721 gagcggcgat cgttccgaca acctgaccaa gtaggatctc gcgggaaggc aatttgccca
      781 tggcgatgat gtccgtcgcc ggaaccgctt ttccggccgc gatgtcgaat cctccgagca
      841 ccttgaagcc ttcggctttg tagatcgggc cggaaacggc gaaaacgtcg gtcggagaaa
      901 aaaccgtgcc gacctggcct tcgaaagttt ccggatcgat agcgacattc gctctcgaga
      961 agatcaactt gagcagcctt tttttgacga cgtcaaaatg tccgcccagt tttctcatct
     1021 cgcttctgaa agcgttgagt ttacccgtgg gaacgccggt gaaatccaaa aaaaccatcg
     1081 actggctgga tttcacgtcc tccgttccct gtctcaagat ctcctgtttt tcttttctgg
     1141 taagcatgtt tgttttcccg aaagggaaat aaaaaactgc gctataaacc cgcagtcgac
     1201 ccgaatgctt tcctcggcag gttcctttga aggaattaag gcgaacgcct gctttctgcg
     1261 gatatggctt atcttctcac gatttcccta ctcttgtcaa ttcttggatt tcacgagcgg
     1321 cacgaagctg aagccgaaat actctttggt ttcgaattgg tcgggtttga ttttgtcgat
     1381 gacgacgatg ctctgcccga ccggagcgac gatgcggccg ccgaccacca gttcttcttt
     1441 ccaagcctcg ggaatttcct cggccgcggc cgcggcgatt atcttgttga aaggcggctc
     1501 gccttccatt tttctggaac cgtctccgac gacgattttc acccggcccg aatcgatgaa
     1561 tccgtatttt ccgatattct ctttggcttt ttccgcaagc tccttgatgc gttcgattcc
     1621 gaccacttgt ccggtttctc cgaccaaatc ggcgaggatc gccgcttgcc agccgctgcc
     1681 gcttcccaca tcgagcacgc gttcaccggg cttaggccgc aaaagctcga tcatgaaagc
     1741 gacgaccaaa ggctgggata tggtctgatc aaagccgatg gccaaagccg aatcgaaata
     1801 agcttcttca ccgagttccg gaggcacgaa atctttccgg tcgattttcc ggaaagcttc
     1861 ggcgataacc ggagtttgga tataaccatc ggtttcgaga tgcctgatga gctcctcgta
     1921 gctttcgttc acaaacccgg agtcgtcttc gttttcaggg ttgttttcct cgaaaatatt
     1981 gattttttca gccatttatg atgtcattat aatataaaat ggtcgtttag gccattttaa
     2041 gttcctaaaa ataagaagga ggaagattgg atttggaaac atcagcggtg gaagaacaag
     2101 cttcggtacc ggcgttccgc gtcaaagtaa cagggactga gacagtggtc atacccgtgc
     2161 ccaacgagga cacgaaagac tttcgagggg tcgtagaagc gatcaaggcc aaggttgccg
     2221 aagcagtcta tcgaaaacca cccaggattg tcgtggcggt gacggcgatc gaaataagcc
     2281 gagccgttgc cgatcactta aagagccaaa ggccgttcgg tcgaaaacaa atcaccgaag
     2341 gcctggttgt tccccagtcg ttgacgcttg tcgtcaatga cgcgcctgtc tttcggacaa
     2401 gcaaaccgtt gagctgcgag cgccacgcgt ttatgacggt tgtcgaacgt atgagtggag
     2461 taaagttgaa agaactgctt tgctccggca gtagcttcgt catctataac caggttg
//
LOCUS       DAOPQT010000081         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_45185_length_2511_cov_3.025651, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000081 DAOPQT010000000
VERSION     DAOPQT010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45185_length_2511_cov_3.025651"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PKG74_02415"
     CDS             <1..915
                     /locus_tag="PKG74_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOB90163.1"
                     /translation="GDTVVVSRAGDVIPQITKVFKDLRTGKEKSFKMPKTCPVDGSPV
                     VKTGVFYRCSNPKCGARNMREIRHFVSKAAFNIEGLGPKIIDRFMDEGLISDAADIFS
                     LQKGDIASLEKFGEKSADNLINEIETRKKTSLARFIYSLGILHIGEETANTLAKDVSK
                     RSGKKTFRPKEAAGLLESLSKEELEELPDIGPKVAESFYSWFKDKRNIHLLEKLDKSG
                     VEIRAEIPASGKLSGKTFVLTGTLETMSREQAKEKIRALGGDISESIGSKTDYLVAGV
                     DPGSKYDKAKEIGTEVLDEKEFLEILRN"
     gene            988..1563
                     /locus_tag="PKG74_02420"
     CDS             988..1563
                     /locus_tag="PKG74_02420"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOB90164.1"
                     /translation="MFDLEGKRVLVRPAETKDLRLLYTWRNQADFIELCSARRRIVGY
                     EEFVEEFHRDLDGDCHIQCVIERLKDQTTIGTVFSYNLNLVDGFVFVTIFVDEHYRRL
                     GYSVEAMALFLDYLFTTLPLHKIYMEVYGYNDASLSVVRRAGFAQEACFKEHRYLDGK
                     RHDLFRFALFRDKSQVVKDFLARLRLRKEGG"
     gene            1869..>2511
                     /locus_tag="PKG74_02425"
     CDS             1869..>2511
                     /locus_tag="PKG74_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90165.1"
                     /translation="MYSRITSNIFWAFLPQKTDYTFLSIRFNLDKTQSQKDFLAIAHL
                     LEHYLVNDVRVKDGPDLYEVQGDVSADCLTIHFRFYKKDAIKSLKDIFNIFQYQSFDN
                     EDLFVQEKKRVISELAAKNDDLFEKLYQISSAERFIGFNPYKEDYNSAIEAIKSIKID
                     DIKKLFSKINKNKITVFSAYKPNIVFRLKTAGLLKSNLSGLNYEVFDASQKMTL"
BASE COUNT          759 a          465 c          624 g          663 t
ORIGIN      
        1 ggggacacgg tggtggtgag ccgggccgga gacgtcatcc cccaaatcac caaggttttc
       61 aaggatttgc ggaccggaaa ggagaaatct ttcaaaatgc ccaaaacttg tccggtggac
      121 ggctcgcccg tcgtcaaaac cggagttttt taccgatgtt ccaatccgaa atgcggcgcc
      181 agaaatatgc gggaaatcag gcatttcgtt tccaaggcgg ctttcaatat cgaaggcctt
      241 ggcccgaaaa tcatcgaccg gtttatggac gaaggcttga tctcggatgc ggccgatatc
      301 ttcagtctcc aaaaaggcga catcgcctcg cttgagaaat tcggggaaaa atcggccgac
      361 aatctgatca acgaaatcga gaccagaaaa aaaacgagtt tggcgagatt tatttattct
      421 ttgggaatcc tccacatagg cgaggaaacg gcgaacacct tggctaaaga cgtgtcgaaa
      481 agaagcggaa aaaagacatt cagacccaaa gaagcggccg ggcttttgga atctctgtcg
      541 aaagaagaac tcgaggaatt gccggatatc ggtcccaaag tggcggaaag cttttattcc
      601 tggttcaaag acaaaaggaa tattcatctg ttggaaaaac tggacaaatc gggagtagag
      661 atccgggcgg agatcccggc ttctggaaaa ttgagcggca agacttttgt cttgaccgga
      721 acgctcgaga cgatgtctcg ggaacaagcc aaagaaaaaa tccgcgcttt gggaggagac
      781 atatcggagt caatcggctc gaaaacggat tatcttgtcg cgggcgtcga tccgggttcg
      841 aaatacgaca aagcgaaaga gatcggtacg gaagttttgg acgagaaaga gttcttagag
      901 atattgagaa attaattttg ctatcatata gtaaagttat aagagagctc ttttccataa
      961 gaatatcgca gaaaggaagg aggtccggtg tttgatctgg aaggtaagcg agttttggtc
     1021 cgacctgctg aaacgaagga tctaagattg ctatatacct ggcggaatca agctgatttt
     1081 atagaattgt gttcagcgcg ccgccgaatt gtcggatatg aagagttcgt cgaagaattc
     1141 catcgggatc tagatggaga ttgtcatatt caatgcgtga ttgaacgcct gaaagatcag
     1201 acaaccatcg ggacggtttt ctcctacaat ctgaatcttg tagatggatt cgtctttgtc
     1261 acaatctttg tagacgaaca ttatcgacgt ttaggatata gcgttgaagc aatggcgctc
     1321 ttcttggatt atctgtttac gacactgcct ctccataaga tttatatgga ggtatatggc
     1381 tataacgacg cttcgttatc cgttgtccga agagccgggt ttgctcagga ggcatgtttt
     1441 aaggagcatc gatatctaga cgggaaaaga catgaccttt tccgtttcgc tctattcaga
     1501 gacaaatcgc aagtcgttaa agactttttg gcacgcttgc gattaagaaa agaagggggg
     1561 tgagattaag tgtctaaagg tggtggaggc ggtggcgatg attctcaaaa agacggagtc
     1621 tatcttgagg gccgtgctag ccattctcac caacatagag atggatcata tggtagagac
     1681 atcgaagctt atcgcaatac ctatgatcat ggtaagttag tgagtagcga caaggctagc
     1741 gattggggca aggttcatac cgacgatcgc agcggtggta gtagcagcgg gaaataagtg
     1801 atttctaaaa ggggcgcgta tgttcgcccc ttctttcttt ggttttttac gctattatta
     1861 gatagtccat gtattcacga atcacttcaa atattttttg ggcattttta ccgcaaaaaa
     1921 cagattacac ctttttgagt attagattca atcttgataa aacgcaatcg cagaaagatt
     1981 ttctggcgat cgcgcatctt ctggagcatt atttggttaa cgatgttaga gttaaagatg
     2041 ggccggattt atacgaagtc caaggagatg tgtcggcgga ttgtttgacg atccatttcc
     2101 gcttctataa aaaagatgcg ataaaatcgt tgaaagatat ctttaatatc ttccaatatc
     2161 aaagttttga taacgaagat ttattcgttc aagaaaaaaa gcgagtgata tcggagttgg
     2221 cggctaaaaa cgacgatttg ttcgaaaagc tttaccaaat atcaagcgcg gaaagattta
     2281 taggattcaa tccgtataaa gaggattata atagcgctat cgaagcgata aaaagtatta
     2341 aaatcgatga tattaaaaag ttattttcga agatcaacaa aaataaaatt accgtattca
     2401 gcgcttataa gcccaatatt gtttttcgat tgaaaacagc cggattattg aagtcgaatc
     2461 tatccggtct taattacgaa gtttttgacg cgagtcaaaa aatgacttta t
//
LOCUS       DAOPQT010000082         2487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_45738_length_2487_cov_3.441201, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000082 DAOPQT010000000
VERSION     DAOPQT010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45738_length_2487_cov_3.441201"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..366)
                     /locus_tag="PKG74_02430"
     CDS             complement(4..366)
                     /locus_tag="PKG74_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90166.1"
                     /translation="MKKTIKNALIDAGLVILYIALVVAFMIRGENALRNETASLIMML
                     LLLVISVAMMGVLIFGKPTLWYLEGKKERSRIPSFIYRRILVYRPDPALRDSLHRVFL
                     TRTAKRSILKQAIKAGRS"
     gene            713..916
                     /locus_tag="PKG74_02435"
     CDS             713..916
                     /locus_tag="PKG74_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90167.1"
                     /translation="MKCFCGRHFHKWGKRSGGSAVYGLGFIGALVYYMGHAASFQTVI
                     LGILKAFVWPAFFVYKAIEIWQI"
     gene            920..1351
                     /locus_tag="PKG74_02440"
     CDS             920..1351
                     /locus_tag="PKG74_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOB90168.1"
                     /translation="MDEYKPRVGIGVMIFKEGKVLLGKRKGSHGEGEYAFPGGHLEYL
                     ESFSDCGRRETREECGIEIKNIRFQYLANVTKYAPKHYAHIGLSADWDSGEPQVLEPD
                     KSESWDWYDLDNLPKPLFEPCALAIRCHKENKNYLDSGDII"
     gene            complement(1385..>2487)
                     /locus_tag="PKG74_02445"
     CDS             complement(1385..>2487)
                     /locus_tag="PKG74_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90169.1"
                     /translation="EKNYKDFLDPDLLEAWKSDPSQALGRITSSPWPERIEIGEISRF
                     DSEAYDISGKIIEMTSTGMSGSRAVQINVAKFGNRWLITGVYVFPYQVDDSWKEYEGN
                     EISFEYPETLGAEYISETEWPPTVEIRSGDDNKCWEGITVSSAYPIYFREVMDKTIYC
                     IDVSEEGAAGSVYKSYTYISPRRGRLVALSFVLRYPNCLNYPEDQARTCAKEREFFDL
                     DALVNNIFQTIKFESASNTLADQIARCLVSSSSEGQEKCDELLKQITDFDSCVMAGFP
                     VTKSDSLTQCQVGGKIFTEGENSSWEEVVSAINNCEAATVVPSWRLLVTVILKNDRQL
                     IAVEPERDDILDVTAAAEAKCGPIEIIPLPSD"
BASE COUNT          650 a          601 c          586 g          650 t
ORIGIN      
        1 tttttaactt cgaccagctt ttattgcctg ctttaaaata ctacgtttcg cggttcgtgt
       61 caaaaagacc cggtgtagag aatcgcgtaa agcaggatca gggcggtaaa caagaatccg
      121 acggtatata aaagaaggaa tccggcttct ttcttttttt ccttccaaat accaaagcgt
      181 cggttttccg aaaataagca ctcccatcat cgcgaccgaa atcacgagga gcaaaagcat
      241 cataatcaag gaggcggttt cgtttctcaa ggcgttttct ccccggatca tgaaagccac
      301 caccaaagct atgtataaaa tcactagtcc ggcgtcgatc aaggcgtttt tgattgtttt
      361 cttcattaat tcattatagc aaacaaaact tcttgaaggg ttgccacgcc cgatttttcg
      421 tgttatactt acaaaagtaa ggatctcgcg gtatagtcgc accccgcgaa aaagaagata
      481 aagaaactat cgaccatact atgtacaagt gcgaaaaatg cagcgccact tccgaagctg
      541 cggccgaatg ctgcggtgag aacatgaagg aagtcaaaga agccgccccg gaagcttaac
      601 tttatcatca gaaatctcct cccatcgaac gggaggagat tttttgtttg cggcgtgtta
      661 atattcaacc atggaaccaa atacatacga ggaggcaaga ggtttcgcga gagtgaaatg
      721 cttttgcggt cgccatttcc acaaatgggg taaaaggtcc ggcggatcgg cggtttacgg
      781 gctcggattc atcggcgccc tggtttatta catggggcac gccgcgagtt tccaaaccgt
      841 aatcctgggg attctcaaag cctttgtctg gccggcgttt ttcgtctaca aggcgatcga
      901 aatctggcag atctaagtca tggacgagta taaaccccgg gtcggcatcg gagtgatgat
      961 tttcaaggag gggaaagtct tgctcgggaa gcgcaaaggt tcccacggcg aaggggaata
     1021 tgcttttccg ggggggcatt tggaatatct cgagtctttt tccgattgcg gccgcaggga
     1081 aacccgcgag gaatgcggaa tcgaaataaa aaacatcaga ttccagtatc tggccaacgt
     1141 gaccaaatac gctccgaagc attacgccca catcggcctg tcggccgact gggattcggg
     1201 agagccgcaa gttctcgagc cggacaaatc cgagtcctgg gactggtacg atttggacaa
     1261 tctgccgaag ccgcttttcg agccgtgcgc tttggctatc cgatgccaca aagaaaataa
     1321 gaattacctg gattccggag atatcatttg attcaaaaaa tcccgctatt agcgggattt
     1381 tatcttaatc gcttgggagc ggaattatct cgatcggacc gcatttggct tcagccgccg
     1441 ccgtgacatc gaggatatcg tccctttccg gctcgacagc gatcaattgc ctgtcgtttt
     1501 ttaaaatgac ggtgacgagt aacctccacg atggaacaac agttgcggct tcgcaattat
     1561 tgatcgccga gacgacttct tcccaagaac tgttttctcc ttcagtaaaa atctttcctc
     1621 ctacttgaca ttgagttaaa gaatcagatt tcgttactgg aaatccggcc ataacgcagg
     1681 aatcgaaatc cgtgatttgc tttaaaagct catcgcattt ttcttggcct tcggaggagc
     1741 tcgacaccaa gcagcgcgcg atctgatccg ccagggtatt cgacgccgat tcaaatttaa
     1801 tcgtttggaa gatgttgttt accaaagcgt cgagatcaaa aaattccctt tctttcgcgc
     1861 aggtccgggc ttgatcttcc ggataattca ggcaattggg atatcggaga acaaaactca
     1921 atgcgaccag ccgtcctctt cttggagaaa tgtaagtata agacttataa acgcttccag
     1981 ccgcgccctc ctccgaaacg tcaatacaat aaattgtttt atccatcact tctcgaaagt
     2041 aaatcggata cgcggatgat acagttattc cttcccagca tttattgtca tctccggatc
     2101 ggatttctac tgtcggcggc cactcggtct cagaaatata ttcggctccc aaggtttccg
     2161 gatactcgaa agaaatctcg ttgccttcat attctttcca agaatcgtcc acttgataag
     2221 ggaaaacgta gactccggtg ataagccagc ggttgccgaa tttggcgacg ttgatctgga
     2281 ccgcccgcga tccggacatg cccgtgcttg tcatctctat gattttaccg ctgatgtcgt
     2341 aggcttcgga gtcgaatctc gaaatttctc cgatctctat tctttccggc caggggcttg
     2401 aggtgatcct tcccaaagct tgcgagggat cggatttcca agcctcgagc aaatctggat
     2461 cgagaaaatc cttataattc ttttcta
//
LOCUS       DAOPQT010000083         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_46077_length_2474_cov_4.014882, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000083 DAOPQT010000000
VERSION     DAOPQT010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46077_length_2474_cov_4.014882"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..356
                     /locus_tag="PKG74_02450"
     CDS             78..356
                     /locus_tag="PKG74_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90170.1"
                     /translation="MELIDAAIGLMLIYVSIEIALSSALEWFPDIRFLTSIDKSVLFR
                     AFRAALAFTIAIIFLAIFISSSLEERSCSYIIGSVLHFAFSFLILKRE"
     gene            457..531
                     /locus_tag="PKG74_02455"
     tRNA            457..531
                     /locus_tag="PKG74_02455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:490..492,aa:Arg,seq:tcg)
     gene            complement(556..867)
                     /locus_tag="PKG74_02460"
     CDS             complement(556..867)
                     /locus_tag="PKG74_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90171.1"
                     /translation="MKIEIKWWVVCVIVWTACFLVVDFSIQDFREDRRQLEEIYWWRT
                     MALSRVEMKIDEDTIPGFGIVYFEGGDKALVLPKRSGGESFFVDLKLRRLDNGKIIDT
                     K"
     gene            complement(1670..1927)
                     /locus_tag="PKG74_02465"
     CDS             complement(1670..1927)
                     /locus_tag="PKG74_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90172.1"
                     /translation="MLARVRTFVVGTLPREISREVLLRLYALGADLQRSFASSSDFVP
                     GEKVEIVFHKDLPEPEMRRICLSFVRRVKKLEPRFEEQEFS"
     gene            complement(2021..2236)
                     /locus_tag="PKG74_02470"
     CDS             complement(2021..2236)
                     /locus_tag="PKG74_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HOB90173.1"
                     /translation="MDMDVKPGKYKHFKGFDVEVIGCGKHTETLEEFVIYRDHLCGDL
                     WVRPKAMFTDIVERNGETKPRFRYLDN"
     gene            complement(2288..>2474)
                     /locus_tag="PKG74_02475"
     CDS             complement(2288..>2474)
                     /locus_tag="PKG74_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90174.1"
                     /translation="DRVWKKTHDAASKIFTWLGTLTLASLFFRPEIIFAVIIGGTLIA
                     ALYLFAYSYLEFRKENR"
BASE COUNT          636 a          600 c          514 g          724 t
ORIGIN      
        1 cgacgtaaaa tgttctatag agatgacaga aattgagcta taaagctcat gtacattccc
       61 agaaaggaag gatcaccgtg gagttaatcg atgcggctat cggcctaatg ttgatttatg
      121 tctctatcga gattgctttg tcctctgccc tcgagtggtt tccggatatt cgtttcctta
      181 cgtctatcga taaatctgtg ttgtttcgtg ctttccgcgc cgctttggct tttactatag
      241 cgatcatctt tcttgccatt tttatctctt cttctttaga ggaaagaagt tgttcttaca
      301 tcatcggttc agtgttgcat ttcgccttct cgtttctgat tctaaaaaga gaataatcca
      361 agctataagc tctttgtgga tccaatatcc atgaggagtt ttttgttttt ttaaagccta
      421 tcgttttcgg acttttattt gttagaattg gatgacgctc ctgtggtgaa acggatatca
      481 cggctggctt cgaaccagct gttgggggtt cgattcctcc cgggagcacc aaagaaaaag
      541 gaccttaaaa ggtccttact ttgtgtcgat gatttttccg ttatcgagcc ggcgcaactt
      601 taagtcgacg aaaaacgact cgcccccgga tcgtttggga agcaccaagg ctttatctcc
      661 accttcaaaa tagactatcc cgaagccggg aatggtatcc tcgtctatct tcatctccac
      721 gcgggataaa gccattgtcc gccaccaata aatctcctct aattgcctcc tgtcttctct
      781 gaagtcttga atggagaaat ccacgacaag aaaacaagct gtccagacga tcacgcaaac
      841 cacccaccac ttaatctcga tcttcattgt tcccttcggc cacatgagtc ttgccccccc
      901 tctttaggtt ttcgaggtac ttctatgatt tcaacctaaa acaacgtcga agattgtcaa
      961 ttcactttaa cgaaatcgga aagcccctct tataaagagg ggctctaaat gctttattgt
     1021 ttaaatccac gatcgatatc ctgaatttaa agataaaggc tagattgagg ttctagccgg
     1081 acctttttct actagtgtcc gtagtatggt gtaatctctg ttataccatg tggagtttcc
     1141 accacgaggc cgccgtcggc tttctctgta actgtgattc tttcttcttt taataaagaa
     1201 gaaagatctt gaagcaatcg tatcctatgc accaatccta tctgcaatgc gtcgatctga
     1261 gcggtcaact tgtcaatatc atcttgaagt cgagagactg aaacggccga caccgccaaa
     1321 acaatgacaa tggcggctaa ggctgctacc gtagaactaa tcttataaat taattataac
     1381 ataattgtta tgatttgtca agagagatcc attaaacaaa tccccgcctt acgacggggt
     1441 tgatatagcc ggatggatct ttatcggagc gtcgggatca aagaaacctt ctgctttctc
     1501 caaccccact ttgaccctca taaactccgc ggcctccatg ggattgccgc aggctttaca
     1561 gatcgatggc gtgaagtttt ggtgaataaa gccgcactcc ggacaaacat atttggttat
     1621 cgatttgatg ccggacacat tgtcacctcc tttcgaaaaa acggttggcc tacgagaatt
     1681 cctgctcctc gaatctcggc tcgagcttct taactcttct tacgaagcta aggcagatcc
     1741 gtctcatttc cggttccggc aaatctttgt ggaaaacgat ctccactttc tcgcccggga
     1801 caaaatcgga tgaagacgcg aatgaacgtt gcaaatcggc tccgagagcg tacaaacgca
     1861 gtagcacttc gcggctgatt tcccggggca aagtaccgac aacaaaagtt ctaacacggg
     1921 ccaacattat cttcgccccc tttttgaaaa gatcgatctg ctttacttat aacttaacgg
     1981 ctgcggttta ggcaaccgcg gtcgtttttt ccattaacac ctaattgtcc agatatctga
     2041 atctcggttt tgtctctccg ttgcgttcca cgatgtccgt gaacatcgct ttcggtctga
     2101 cccaaagatc gccacagaga tgatcgcgat agatcacgaa ttcttccaaa gtttcggtgt
     2161 gcttgccgca gccgatgacc tcgacgtcga atcctttgaa atgtttatat ttccccggct
     2221 ttacgtccat atccatattg gatattttag cacgctatga tcagcgcagc tacgcgatta
     2281 ccggaattta tcggttttct ttccggaatt cgagataaga ataagcgaaa agatacaagg
     2341 ccgcgattaa tgttcctccg atgatcaccg cgaaaatgat ttccggccgg aaaaacagac
     2401 tagcaagagt caaagttccg agccaggtga agatttttga ggcggcatca tgagtctttt
     2461 tccaaacccg gtcg
//
LOCUS       DAOPQT010000084         2469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_46215_length_2469_cov_4.006628, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000084 DAOPQT010000000
VERSION     DAOPQT010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46215_length_2469_cov_4.006628"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..1106)
                     /locus_tag="PKG74_02480"
     CDS             complement(282..1106)
                     /locus_tag="PKG74_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOB90175.1"
                     /translation="MTPLNGSLISRDIYERIKPIVPENKFLAAILVGDNPASLSFVKK
                     QKQQAAEKTGIDFRVFEFPESVLEKDLVSAIQNLAADRNCGGIIVQLPLPDHLDKQAI
                     LDAVPREKDPDVLSSESIKLFREGKSLVLPPAVGVVKEILDFQKIDIKQKSAAIIGLG
                     FLVGRPVSDWLRGKCRELFLIDKGDDFSPVKTADIVILGTGVPGIVKESEAKPGALVI
                     DFGYGKDKNGKLKGDFMVADAETKEGFYTPTPGGTGPVLVARLLLNFCELNQNPGN"
     gene            complement(1482..2469)
                     /gene="glyA"
                     /locus_tag="PKG74_02485"
                     /pseudo
     CDS             complement(1482..2469)
                     /gene="glyA"
                     /locus_tag="PKG74_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
BASE COUNT          607 a          586 c          591 g          685 t
ORIGIN      
        1 tgatgtaaat cgtgtttacg ggagcaccgg actcgattac aaagcttcct ccgccaccgg
       61 gaggattttg agtcgggttt tgccaagcaa aagccaccga tttttcgatg gcaaagataa
      121 ataaagctgc gataagggtt ttttggagaa aagttaatcc tttaaccctc cctccccccc
      181 ggatttgagg tgtttttatg gctgatcatg gtttggtgaa gaaattcttt tatattggag
      241 ttattatagc atctttcccg taggccatga attcaggaaa tctaatttcc gggattttga
      301 ttcaattcgc agaaattgag aagcagtcgg gccacgagaa cgggaccggt gccgccggga
      361 gtgggtgtat aaaatccttc ttttgtttcc gcgtcggcga ccatgaaatc ccctttcagt
      421 ttcccgtttt tatcttttcc gtatccgaag tcgatgacca aagcaccggg cttcgcttcc
      481 gattctttga ctattcccgg aacgccggtt cccaaaatca cgatgtccgc ggttttgacc
      541 ggagaaaaat catctccttt gtctatcaag aaaagctcgc ggcatttgcc acgaagccaa
      601 tcgcttacgg gccggccgac caggaatccc aaaccgatta tggccgcgga tttttgctta
      661 atatcgatct tctgaaaatc caagatttct ttgacgactc cgacggccgg aggcagaacc
      721 aaagacttcc cttcccggaa aagcttgatc gattccgaac tcaaaacatc cggatctttt
      781 tctcgcggca ccgcgtcgag aatcgcctgc ttgtccaaat gatccggcaa aggaagctga
      841 acaatgattc ctccgcaatt tcgatcggcg gccaggtttt ggattgcgga aaccaagtcc
      901 ttttccaaaa cgctctccgg aaactcaaaa acccggaaat cgataccggt tttttccgcg
      961 gcctgctgtt tttgtttttt aacgaagctc aaagaagccg gattgtcgcc gacgaggatc
     1021 gcggccaaaa acttgttttc ggggacaatt ggcttaatcc gttcgtaaat atcccggctg
     1081 atcaaagagc cgttaagcgg ggtcataaaa taagcatagc ctacaaaagc tttgtcttaa
     1141 agaatcttag ttcgcttgcc aaaacttata aaatatggta tttattaaaa tataggaggt
     1201 gaaattaatg gaagttcttt ctttaaatat cgttgacgtt cgcagaagac gaaagagcca
     1261 agaacgcctt tctgatgatg cgtctgcgtt tctccatact gaaacggtat tggaacgcta
     1321 catcgacgat aagaatccta gttatagcca gaattcaaag gcgtatcagg atctcagtcg
     1381 ccggcgagcg gcggaaagac gccagtatcc tcggtcctag tggcccgaag ccgcccacca
     1441 ctttctaaac agagtgtcgg gcggtatttt tttgtctaac ttctgttttt caaaatatcg
     1501 taaatcttct tggcgatcgc tttcatgtct ttttccttca ggccgcggcg cgtgaccgcc
     1561 ggagtgccga ttctgattcc ggacggcttg aaagccgaaa catctccggg aatgctgttc
     1621 cggttcgcga gaatatcgtt tttctcgagc agtttttccg cggtcagtcc atcgatattg
     1681 aaattcttca aatccaaaag catcaaatgg ttgtccgtgc ctccggtcac caagtcgaat
     1741 ccgagttttt tcaactcttc ggccaaagct ttggcgtttt tcaggatttg ctcctcgtat
     1801 ttcttgaatt gcggcttttg ggcttcgtag aacatctggc atttggccgc ggtgacattg
     1861 ttgtgcggac cgccttggag tccgggaaaa accgctttat cgattttctc ggaaatttct
     1921 ttcttggaaa aaatgatcgc ccctcttgga ccggccaaag ttttatgggt agttgtcgta
     1981 acgacatcgc tatacggaaa aggcgaagga tggagtccgg ctaaaaccaa tccggcgata
     2041 tgggaaatgt cggccatatg gtaagcaccg acggatttgg cgatttctcc gatatgcttg
     2101 aaattgattt ttcggggata agcggaaatg ccggagacga ttatcttcgg acggtgtttt
     2161 gcggccagac gttcgatctc gtcgtaatcg ataagcccgg ttttctcatt aacgccgtat
     2221 tgaaccgaat taaagaattt tccggaaaaa gaaaccttgt ggccgtgggt taaatggcct
     2281 ccggccgcga gcttcattcc caagatcgtg tctccgggtt ccaaaagccc gccgtagacc
     2341 gcgaaattcg ccggagagcc ggaataaggc tgaacattca cggaccattt cgagggattc
     2401 aagcggaaag cttttaaggc gttttcctgg cacagttttt cgatttcatc gtaatactcg
     2461 tttcccggg
//
LOCUS       DAOPQT010000085         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_47137_length_2432_cov_3.496424, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000085 DAOPQT010000000
VERSION     DAOPQT010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47137_length_2432_cov_3.496424"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..894
                     /gene="rpsB"
                     /locus_tag="PKG74_02490"
     CDS             127..894
                     /gene="rpsB"
                     /locus_tag="PKG74_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOB90176.1"
                     /translation="MAIQTETKENEIIETQEAKVSPEDLLTLQEMIDAGLIHGLAKSK
                     THPKMKPYIHSTRSGVEVIDLVQTISLLKKASEFLKSVVDSGKSVLLVGTAAIAKQGV
                     KNSAEKTGNPYVIERWIGGTLTNFKIVNGRVQHFKKLLADKEAGNLAKYTKKEQLMIE
                     REIAKMAAIFTGLKDMNELPGAMLVMSPSENKLAIAEAKNAGIPVVAFLNTNADPTTV
                     EYPIPGNDKLSTSIEWVLNYLEPNLKRNNERGNSQTA"
     gene            866..1309
                     /gene="tsf"
                     /locus_tag="PKG74_02495"
     CDS             866..1309
                     /gene="tsf"
                     /locus_tag="PKG74_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOB90177.1"
                     /translation="MNAETVKRLREETGAGVMDAKRALEDAAGDFEKAKALIAERGLA
                     KAEKKAERTTGSGRIFSYIHNERIGVLLLLRCETDFVARLEDFQELGRNLTMQIAAME
                     PETTGDLLAAPYIRDENTTIDNLIKGVIAKTGENIQVEKFCRYVL"
     gene            1329..1616
                     /locus_tag="PKG74_02500"
     CDS             1329..1616
                     /locus_tag="PKG74_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90178.1"
                     /translation="MMFSLAAAVYVFSRAIPRVDDEVVAPGEKKDGLWWERTMKKLPL
                     DKIDDTFNRFVEKTLRKTKIAIMKADNAVTGKLKGFKSENGKKNGDSGLIS"
     gene            1694..1777
                     /locus_tag="PKG74_02505"
     tRNA            1694..1777
                     /locus_tag="PKG74_02505"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1728..1730,aa:Tyr,seq:gta)
     gene            1867..1938
                     /locus_tag="PKG74_02510"
     tRNA            1867..1938
                     /locus_tag="PKG74_02510"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1899..1901,aa:Thr,seq:tgt)
     gene            1951..2022
                     /locus_tag="PKG74_02515"
     tRNA            1951..2022
                     /locus_tag="PKG74_02515"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1983..1985,aa:Thr,seq:ggt)
     gene            2050..2226
                     /gene="rpmG"
                     /locus_tag="PKG74_02520"
     CDS             2050..2226
                     /gene="rpmG"
                     /locus_tag="PKG74_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOB90179.1"
                     /translation="MAKAKSKFSENLIKLKCGVCGKINYFTRKNKKLVERKISLQKYC
                     SRCRKRTEHKEVKK"
BASE COUNT          708 a          574 c          622 g          528 t
ORIGIN      
        1 aaagtgccga aagtcctgat ccagggagat caccaagcaa tcgccgactg gcggcttaag
       61 gaggggaaga aaaagctcgg aaaagttgac taaaaagccg tttttaatta ctattgttta
      121 gtatttatgg cgattcagac cgagaccaaa gaaaacgaaa taatcgaaac ccaggaggcc
      181 aaggtgtctc cggaagactt gttgacactt caggagatga tcgacgccgg gcttattcac
      241 ggcttggcca aaagcaagac ccatccgaaa atgaagccgt atatccactc gacgagaagt
      301 ggagttgaag tgatcgatct tgtccagacg atcagtcttc ttaaaaaagc gtccgagttc
      361 ttgaaatcgg ttgtcgattc cggaaaaagc gttcttctgg tcggaaccgc agccattgcc
      421 aagcaggggg ttaaaaactc cgccgaaaaa accgggaatc cctacgttat cgaaagatgg
      481 atcggcggaa cgctcaccaa tttcaagatc gtcaacggcc gggtccagca tttcaagaaa
      541 ttgctggcgg acaaggaagc cggcaatttg gcgaaataca ccaaaaagga acagctgatg
      601 atcgaaagag aaatcgccaa gatggcggca atcttcaccg gactcaaaga catgaacgag
      661 cttccgggag cgatgcttgt catgagcccc tcggaaaaca aattagccat cgccgaagcg
      721 aagaacgccg gcattccggt agtggctttc ttgaatacca atgccgatcc gacaacggtc
      781 gaatatccca ttccggggaa cgacaagctt tcgacctcca tcgaatgggt tttgaattat
      841 ttggaaccta accttaaacg caacaatgaa cgcggaaaca gtcaaacggc ttagggagga
      901 gaccggcgcc ggcgtgatgg acgcgaaacg cgccctcgag gacgcggccg gggatttcga
      961 aaaagccaaa gctttgatcg ccgaacgcgg attggccaaa gccgagaaaa aagccgaacg
     1021 aactaccgga agcggccgga tcttttctta tatccacaac gaaaggatcg gcgttctgct
     1081 tttgctccga tgcgagaccg atttcgtggc gcgcctcgaa gatttccagg aactcggccg
     1141 caaccttacg atgcagatcg cggccatgga gccggagacg acaggggatt tactcgccgc
     1201 tccctatatc cgcgacgaaa acaccaccat cgacaatttg attaaaggcg tcatcgccaa
     1261 aaccggcgaa aatatccagg tggagaaatt ctgccgctat gttctataac ttcttgctcc
     1321 agataataat gatgttctct ctcgctgcgg cggtttatgt tttcagccgc gccattccgc
     1381 gcgtggacga cgaagtcgtt gcgccgggcg agaaaaaaga cggcttgtgg tgggagcgga
     1441 cgatgaagaa actgccgctc gacaagatcg acgacacctt caatcgcttt gtcgagaaaa
     1501 ctttgagaaa aacgaagatc gccatcatga aagccgataa tgcggttacc ggaaagctca
     1561 agggattcaa atcggaaaac ggcaagaaga acggcgatag cggtttgatt tcttagcccg
     1621 aaatctgctt aaataaaaca agcctatcaa ggcttgtttt gaaacattca gtcgtttcaa
     1681 acattaatcg gacgctgagg tggcggagcg gtcaatcgca acagactgta aatctgtcgc
     1741 ccttgtggct acgggggttc gaatccctcc ctcagcacaa ataatcgttt tcggcgatta
     1801 tttggcggaa taagagaaaa atcgtattaa aataggcttt gcgttttcga gagactttgc
     1861 tattatgcca gcatagctca gtggcagagc aactgttttg taaacagtgg gtcgggggtt
     1921 cgattccctt tgctggctcc gggctttaca gccgatgtag ttcagtggta gaacgctttc
     1981 ttggtaagaa agaggtcgtg agtccgattc tcaccatcgg ctcaaaggag cgaaaaaaaa
     2041 taaaccaaca tggctaaagc aaaatcaaag ttttcggaaa acctcataaa attgaaatgc
     2101 ggcgtttgcg gcaagatcaa ttatttcacc cgcaagaata aaaaactggt ggaaagaaaa
     2161 atcagtctcc agaaatattg cagccgctgc cggaagcgca ccgaacataa agaggtcaaa
     2221 aaataatcgt ttcgatatga aaaacaaaac cccgccattt ctggacgggg ctttggattt
     2281 ggttcgcggt cttagcgtcg gccgacgaaa gagaatatcc cggcctgaga gagccgcttg
     2341 gtttccgact tatcccgttc attacgacga gacccgtttt gctttacgcg ttcttcgccg
     2401 gagtctttga tcttgtcata actgcggcaa tc
//
LOCUS       DAOPQT010000086         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_47397_length_2423_cov_3.504223, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000086 DAOPQT010000000
VERSION     DAOPQT010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47397_length_2423_cov_3.504223"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="PKG74_02525"
     CDS             complement(<1..1203)
                     /locus_tag="PKG74_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90180.1"
                     /translation="MDSQQNFPSNLSPNQNQSTPPFGAAPSSFNPAPFGSTPASMPAP
                     TDQFPQPSAPVNQAFNIPPQPASFTQPSQPQPMNQPFVPASPAPATPVPPPPSSPDIG
                     VRTMASDAESLRVTGGSEAIPQILSTSPAGPNEPVFTPDVVNLAGGSKPGSPRRSRRW
                     LTTIIVGLVLILGVFLAFRYVVLPRMCPVCEICTPVEEPVIPPPEEVVVPTRAHTSYF
                     TIAPDRTENIQLDTVSLLNIKERLSQIPSEMIQPSTVIETVISDSAGPITLPIYFNTV
                     FPGDIPEPDLVANFEEDFTPFVYYSASSNPFPGIIARVRPGISADTYEAIGTRFEGSS
                     QFANLFLTDTGNFGSFDDSSIDPTKLVRFARGSNSQRIEYGFFQSGENTYWVITTSYD
                     AIKEAVRRL"
     gene            complement(1229..>2423)
                     /gene="pnp"
                     /locus_tag="PKG74_02530"
     CDS             complement(1229..>2423)
                     /gene="pnp"
                     /locus_tag="PKG74_02530"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOB90181.1"
                     /translation="ELVHKNILEKEMRPDGRKLDEVRDLYTETGLLPRTHGSALFIRG
                     NTQALAVTTLAAPGAEQLIETMEYSGKRRFMLHYNFPPYSVGETGSYRGPGRRDIGHG
                     ALAEKALRPLIPTQDEFPYTIRVVSEILSSNGSSSMATVCAGSMSLMDAGVPIKKPAA
                     GIAMGLMSNEKGDYKILTDIQGPEDHHGDMDFKVAGTTDGVNAVQMDVKIWGVTLEIV
                     EKTFVQAREARTRILESMMKTIKSPKELSPYAPQVMVIHIDPDKIGEVIGPGGKTINA
                     IIERTKALTIDIEQTGKVFVAADTKEAVLKAVAEIEGLTKEYEVGEIVEGNVVRLLDF
                     GAILDLGGGRDGMIHISEFKDGFVKNINDVVKIGDFLRAKVVRVEDGKIALSVKQLST
                     QSKQK"
BASE COUNT          540 a          620 c          685 g          578 t
ORIGIN      
        1 gagccttctc accgcttctt tgatggcgtc gtaggaagtg gttatgaccc agtaggtgtt
       61 ttctccggat tggaaaaacc cgtattcgat cctttgagaa ttggatcccc tggcaaaacg
      121 gacaagcttg gtcggatcga tgctgctgtc gtcgaaactc ccgaaattgc cggtgtcggt
      181 caaaaagagg ttggcgaact gagaagatcc ctcaaatctg gtgccgatgg cttcgtaagt
      241 atcggccgag attccgggcc tcacccgggc gattattccc ggaaaaggat tggaagaggc
      301 gctgtaataa acgaacgggg tgaaatcctc ttcgaaattg gcgaccaagt ccggttccgg
      361 aatgtctccc ggaaacacgg tgttgaaata aataggcaaa gtgatcgggc ctgcgctgtc
      421 cgaaatcacc gtttcgatga cggttgaagg ctggatcatt tcgctcggaa tctgggaaag
      481 cctttctttg atgttcaaaa ggctgactgt gtcgagctgg atattctctg tgcgatccgg
      541 tgcaatggtg aaatacgaag tgtgcgccct tgtcggcacg accacttctt ccggaggcgg
      601 aataacgggt tcttcgactg gcgtgcaaat ttcgcaaacc gggcacattc tcggcaaaac
      661 cacgtatcgg aaagccaaga acactcccaa gataagcacc aagccgacaa tgatggtcgt
      721 aagccaacgc ctcgatcttc tcggagatcc gggcttggaa ccgccggcaa gattcaccac
      781 gtccggggta aagaccggtt cgttcggtcc ggccggagaa gtcgaaagaa tctgagggat
      841 ggcttcgctg ccgccggtca cgcgcaagga ttccgcgtcc gaggccatgg tcctaacacc
      901 gatatcggga gatgacggag gcggaggcac cggagtggcc ggagccgggc ttgccggaac
      961 aaacggttgg ttcatcggct gcggctgact cggctgggtg aacgaagccg gttggggagg
     1021 aatattgaaa gcctgattca ccggagccga aggctgcgga aactgatcgg tcggagcggg
     1081 catcgaagcc ggcgtcgatc cgaaaggagc cggattgaac gacgacggag ccgcgccgaa
     1141 cggaggagtc gattgatttt gattgggcga cagattcgaa ggaaaatttt gttggctatc
     1201 catacactaa tgatagcaca acgaaaaatt atttttgttt ggattgtgtg gataattgct
     1261 tcaccgagag agcgatcttg ccgtcttcga cgcggaccac tttcgcccga agaaaatctc
     1321 cgatcttgac gacgtcgttg atgttcttca cgaagccgtc tttgaattcc gaaatatgga
     1381 tcatgccgtc gcgcccccct cccaaatcga ggatggctcc gaaatcgagt aatcgaacaa
     1441 cattcccttc gacgatttct ccgacttcat attccttggt caggccttcg atttccgcca
     1501 ccgctttcag gacggcttct ttggtgtcgg cggccacgaa aacttttccg gtctgttcga
     1561 tatcgatggt caaagccttg gtcctttcaa tgatggcgtt gatcgttttt cctccgggac
     1621 cgatgacttc gccgatttta tccggatcga tgtggatgac catcacctgc ggcgcgtacg
     1681 gagaaagctc tttcggagat ttgatggtct tcatcatgga ttcgagtatc cttgtccgcg
     1741 cttcccgcgc ctggacaaag gttttttcga cgatttccaa tgtaactccc cagattttca
     1801 cgtccatctg gacggcattc acgccatcgg ttgttcccgc cactttgaaa tccatgtctc
     1861 cgtggtggtc ttccggaccc tggatatcgg tcaaaatctt gtaatcgcct ttttcgttcg
     1921 acatgagccc catggcgatg ccggcggccg gttttttgat gggaacgccc gcgtccatca
     1981 aggacatgct tccggcgcag accgtggcca tggaagaaga accgttcgaa gaaagaattt
     2041 cggaaacaac gcggatggta taagggaatt cgtcctgcgt cggaatcaac ggccgcaaag
     2101 ctttttccgc cagagcgcca tggccgatgt cgcgacgtcc cggtccgcgg taagatccgg
     2161 tttcaccgac cgaatacggc gggaaattat aatggagcat gaagcgcctt ttaccggaat
     2221 attccatggt ttctataagc tgttcggctc cgggcgcggc cagagtggtc acggccaaag
     2281 cctgggtgtt gccgcgaatg aaaagggccg atccgtgggt ccgaggcaaa agtccggttt
     2341 cggtgtaaag atccctcacc tcgtcgagtt tgcgtccgtc cggacgcatt tctttttcca
     2401 aaatgttttt gtggaccagt tcg
//
LOCUS       DAOPQT010000087         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_47697_length_2412_cov_3.184132, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000087 DAOPQT010000000
VERSION     DAOPQT010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47697_length_2412_cov_3.184132"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /locus_tag="PKG74_02535"
     CDS             complement(<1..1902)
                     /locus_tag="PKG74_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOB90182.1"
                     /translation="MDDFKSLKLKIASPEEVLKWSYGEVTKPETINYRTQRPEKDGLF
                     SERIFGPTKDYECYCGKYKKIRYKGVVCDKCGVEVTRSSVRRERMGHIKLAAPVAHIW
                     FLRSVPSRIGLALDAPVAKLEKVIYYAAYIITSVDEEARKAAIEEVDREFKSRKKLEE
                     IDELGMAAEVAKQTLKELRPGTILSEAEFYNLSRRFGNVFQARAGAEGVRKVLENLDA
                     EKEIKVIEKELDKTDDTSRRQRLARRLKFFIAMKRNNIRPEWMVMTELPVLPPDLRPM
                     VALDGGRYATSDLNDLYRRVINRNNRLKKLLELKAPEIIVVNEKRMLQEAVDALIDNI
                     ARMGRAQLSARRRPLRSLADMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPNLKYDECG
                     LPKKMALELFRPFVISKIIERGLAYNIKNANRLIESAPAEIWEILEEVIKNRYVLLNR
                     APTLHRLGIQAFKPVLIEDLAIQIPPLVCSGFNADFDGDQMAVHLPLTDEAQKESEFL
                     MLSSRNILKPASGDPIPGPWNDIVLGVFYLTEADPEAKGSGKVFGSKEEAVLAYDSGV
                     VDLRAVIKINKDLETTCGRIIFNRALPEEIGYVNEPINKKAMNQLFARILNECGLEKG
                     KAALDMIQEL"
     gene            complement(1904..>2412)
                     /locus_tag="PKG74_02540"
     CDS             complement(1904..>2412)
                     /locus_tag="PKG74_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOB90183.1"
                     /translation="GATEKEIREELKAAGLPETGQVTVYDGRTGEAFSRPVTVGRIYM
                     MKLNHLVLDKVHMRSIGPYSLITQQPLGGRAQFGGQRFGEMEVWALEGHGAAHTLQEM
                     LTIKSDDVMGRSAAYESIVRGEKIKKPNIPATFNVMVNELKALNLNVELMGDGEEREK
                     PTEETKEN"
BASE COUNT          490 a          672 c          604 g          646 t
ORIGIN      
        1 gagctcctgg atcatgtcga gcgccgcctt gcctttctcg agaccgcatt cgtttaagat
       61 cctcgcgaaa agctggttca tcgctttttt gtttatgggc tcgttaacgt agccgatttc
      121 ttcgggcagg gcgcggttga aaataatccg gccgcaggtg gtttccaaat ccttgttgat
      181 tttgatcacc gcccgcaaat cgacaacgcc gctatcataa gccaaaaccg cctcttcttt
      241 gctgccgaaa accttgccgc tgcctttggc ttcgggatcg gcttcagtca gatagaaaac
      301 tcccaagaca atgtcgttcc aaggaccggg aatgggatcg cccgaagccg gttttaggat
      361 attgcggctc gaaagcatca ggaattcgct ctctttctgg gcttcgtcgg tcaaaggaag
      421 atgcacggcc atctggtcgc cgtcgaaatc ggcgttgaat ccggaacaga cgagcggcgg
      481 aatttggatg gccaaatcct cgatcaagac cggcttgaaa gcctggatgc cgagccgatg
      541 caaagtcggg gcgcggttca aaagcacgta gcgattcttg atgacctctt ccaggatttc
      601 ccagatttcc gccggagcgg attcgatcaa acgattggcg ttcttgatat tgtaagccaa
      661 acccctttcg atgattttgg aaataacgaa tggtctgaaa agctccaaag ccattttttt
      721 cggtaagccg cattcgtcgt atttgaggtt cggcccgaca acgatgaccg atcggcccga
      781 atagtcgacg cgttttccga gcaggttctg cctgaatctt ccctgcttac ccttaagcat
      841 gtcggccaaa gacctaagcg gccttcggcg cgcggaaagc tgggcccggc ccatgcgggc
      901 gatgttgtcg atcagggcgt cgaccgcctc ctggagcatg cgcttttcgt tgacgacgat
      961 gatttccgga gccttgagct ccaaaagctt tttcaagcga ttgttgcggt tgatgacgcg
     1021 gcgataaagg tcgttcaaat ccgaggtggc gtagcggcct ccgtccaaag cgaccatcgg
     1081 ccggagatcc ggcggcaaaa ccggaagctc ggtcatgacc atccattccg gccggatgtt
     1141 attcctcttc atggcgatga agaatttcag tcttctcgcc agtctttgtc tgcggctcgt
     1201 gtcgtcggtt ttatccaatt ctttctcgat aaccttgatt tctttttccg cgtccaggtt
     1261 ctccaaaact ttacgcacac cttcggcgcc ggctctcgcc tggaaaacat tcccgaatct
     1321 gcgggaaaga ttgtaaaact ccgcctcgga aaggatcgtt cccggacgta attcctttaa
     1381 agtctgcttg gccacttcgg cggccattcc gagctcgtcg atctcttcga gtttttttct
     1441 cgatttgaat tcccgatcga cttcttcgat cgccgctttt ctcgcttctt cgtcaaccga
     1501 agtgatgata taggcggcgt aatagatgac tttctcgagt ttggcgaccg gcgcgtccaa
     1561 agcaagaccg atcctcgacg gaacgctcct caaaaaccag atgtgggcga ctggcgcggc
     1621 cagtttgatg tggcccattc tttctcttct caccgaagag cgcgtcactt cgacgccgca
     1681 cttgtcgcag accactcctt tgtagcggat cttcttgtat ttgccgcaat agcattcgta
     1741 atccttggtc ggtccgaaaa tccgctcgga aaaaagtccg tctttttccg gacgctgcgt
     1801 tcggtaattg atggtttccg gcttggtgac ctcgccataa gaccatttaa gcacttcttc
     1861 cggagatgcg attttcagtt ttaaagattt gaagtcgtcc attttagttt tccttggttt
     1921 cttcggtcgg cttttctctc tcttccccgt ctcccataag ttcgacgttc agattcaaag
     1981 ctttcagttc gttcaccatc acgttgaaag tcgccggaat attcggtttt ttgatttttt
     2041 cgcctctcac gatcgattcg taagccgcgg accggcccat gacgtcgtcg gacttgatgg
     2101 taagcatttc ctgcagagtg tgggccgctc cgtgcccttc caaagcccag acttccattt
     2161 ctccgaaacg ctgtccgccg aactgggcgc ggccgcccaa aggctgttgg gtgatcaaag
     2221 aataaggccc gatcgaacgc atgtgcacct tgtccaaaac caggtgattc aatttcatca
     2281 tgtaaattct gccaacggtt accggccggc tgaaagcctc acccgtacgt ccgtcgtaga
     2341 ccgtaacctg accggtttcg ggaagcccgg ccgccttaag ctcctccctg atttcttttt
     2401 cggtagcgcc gg
//
LOCUS       DAOPQT010000088         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_48405_length_2383_cov_2.740550, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000088 DAOPQT010000000
VERSION     DAOPQT010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48405_length_2383_cov_2.740550"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2372)
                     /locus_tag="PKG74_02545"
     CDS             complement(<1..2372)
                     /locus_tag="PKG74_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOB90184.1"
                     /translation="MDGIGSWLLGLTDAAREAWLRMFGDQPEIIGEFPLNPQTPIKEE
                     AIDQYKEKPLISVAKLRIARGETNQIQGSRFSFGGKVTISIERPDKIRIEVPVVSDNS
                     GRIVYQYKPAAYDPIGVYYISAKDTVSGLTSDRIKFELAEAVEKIEEEETSNEVEETE
                     NNKPEDDLRFCDFGSSSYGSGQVILNEIAWMGSSASANSEWIELKNVSTGTVDLSGWQ
                     ILDKDKGIKIRFPAGAKISAGSFYLLERTTDASVPGVAADAIYTGALSNSGEDLKLFN
                     ASCGLVDAVFSSDGWLAGDAASRKTMEKDSSGIGWHTSSFADGTPKQENSTGSYTPPL
                     PPPVTPPSPSPTLVDFCDQFDLDSPTRKILINEIAWAGSIVSSSDEWIELKNIGDTDI
                     SLKGWQLLDKAGDIKIVFPGSTAIPAHGFYLLERTDDESVPGVAANLVYIGGLNNSEE
                     TLRLFDNECALVDEVIDVGENWSNIGGSGAPDYRTAERTNLASWHTYHGEGIIGIKGT
                     PKAENSVPAAEEESDALAADDWQISYNFDEVSVDFSLPINEDVSYRILDMESGKFTDV
                     ILNASGVFSKRIDEIGRNYQYRIRIKGEDGTDLGYSDIKTIYIPSYIENTSFYRASHY
                     TLRGEEIEEPLFEFSYDNYPFMPRDTNLALAYGEPPGPNYKVMVFYLNRDAPKDLFLN
                     VDQPLAKYASDVLKTRYDLCGGITESERSSIVLADAAEGCNIMIGGIPNSAMVFQTYL
                     SEEDNRLLLPIKRQSGETVFDSEDFITVAFYGLYRFYPSSHQETDIFKLLA"
BASE COUNT          554 a          624 c          575 g          630 t
ORIGIN      
        1 gccaaaagct tgaaaatatc cgtttcttgg tgggacgacg gataaaatct gtaaagcccg
       61 taaaaggcga cggtgatgaa atcttccgaa tcaaagaccg tttctccgga ttgccttttt
      121 atgggcaaga gcaaacgatt gtcttcttcg gataaataag tttggaaaac catagcgctg
      181 ttcgggatgc cgccgatcat gatattgcag ccttcggccg cgtcagccag aacaatagac
      241 gatcgttcgc tttccgttat cccgccgcaa agatcatatc tggttttcag aacatcgctc
      301 gcatatttgg ccaaaggctg gtcgacgttc aaaaacagat ctttgggtgc gtcccggttc
      361 aaatagaaaa ccatcacctt atagttcggc cccggaggtt ctccgtaagc caaagcgagg
      421 ttcgtgtctc gcggcataaa cggatagttg tcgtaagaaa actcgaaaag cggttcctcg
      481 atctcttcgc cgcgcagcgt gtaatgggaa gcccggtaaa agctcgtgtt ttcgatataa
      541 ctcggaatat agatcgtttt tatgtccgaa tatcccaagt cggttccgtc ttcgcctttg
      601 atccttatcc gatattggta gttgcggccg atttcgtcga tacgtttgga gaaaaccccg
      661 ctcgcgttca agataacgtc cgtgaattta ccgctttcca tatccaaaat ccggtaagaa
      721 acatcctcgt ttatcggcaa agaaaaatcc acggaaactt cgtcgaaatt gtagctgatt
      781 tgccagtcgt cagccgccaa ggcatcgctc tcttcctcgg ctgccggaac actgttttcc
      841 gccttgggcg ttcctttgat tccgattatc ccctctccat ggtaagtgtg ccagctcgcc
      901 aggttggttc tttcggctgt ccggtaatcc ggagcgccgc ttccgccgat attagaccag
      961 ttttctccca cgtcgattac ctcgtccacc aaagcgcatt cgttgtcgaa aagcctcaag
     1021 gtttcttcgg aattattgag tcctccgata taaacaaggt ttgccgctac tcccgggaca
     1081 ctctcgtcgt cggtccgttc gagaagataa aatccgtgag ccgggatcgc ggttgatccg
     1141 ggaaaaacga ttttgatgtc gccggccttg tcgagaagct gccaaccctt aagggaaata
     1201 tccgtgtcgc cgatgttttt gagttcgatc cattcgtccg aagaagaaac gatcgatccg
     1261 gcccaggcga tttcgtttat gagaatcttt ctggtcggcg aatcgagatc gaattgatcg
     1321 caaaaatcca ccaatgtcgg gctaggactc ggcggcgtta ccggaggcgg taaaggagga
     1381 gtatagcttc cggtggagtt ttcctgtttc ggggtgccgt cggcaaaaga cgacgtatgc
     1441 caaccgatcc ccgaagaatc tttttccatg gtttttctcg aagcggcatc tccggccagc
     1501 cagccgtcgc tcgagaaaac cgcatccacc aacccgcaag aagcgttgaa aagcttcaag
     1561 tcttccccgg aattggacag ggctccggta tagatcgcat ccgccgcaac tccgggaaca
     1621 ctcgcatcgg tcgttcgctc caaaagataa aaacttccgg ccgatatttt ggctccggcg
     1681 ggaaaacgga ttttgatgcc tttgtcttta tcgagaatct gccaaccgga aagatcgact
     1741 gtcccggtcg agacgttctt gagttcgatc cattcgctgt tcgcgctcgc ggacgatccc
     1801 atccaggcga tttcgttcaa aatcacctgt ccgctgccgt aagaagaaga accgaaatcg
     1861 caaaatcgaa gatcgtcttc gggtttattg ttttctgttt cttcgacttc gttggaagtt
     1921 tcttcctctt caattttctc gactgcttcc gcaagctcga atttgatcct gtccgaggtc
     1981 aagccggaga ccgtatcctt ggccgagata taataaactc cgatcggatc gtaagccgct
     2041 ggtttgtatt gataaacaat tcttccgctg ttgtcggaga caacaggaac ctcgatcctg
     2101 attttatccg gacgctcaat tgaaatcgta actttccctc caaaggagaa acgcgaaccc
     2161 tggatttggt ttgtctctcc tctcgcgatc cgaagcttag ctacagaaat cagcggtttt
     2221 tccttatatt gatcgatggc ttcttcctta atcggagttt gtgggttcaa tgggaattcg
     2281 cctatgatct ccggttgatc gccgaacatc cgaagccagg cttcgcgggc ggcatcggtc
     2341 agaccgagca gccagcttcc gataccatcc atgatttgcc cga
//
LOCUS       DAOPQT010000089         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_49860_length_2331_cov_3.275923, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000089 DAOPQT010000000
VERSION     DAOPQT010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49860_length_2331_cov_3.275923"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..195)
                     /locus_tag="PKG74_02550"
     tRNA            complement(109..195)
                     /locus_tag="PKG74_02550"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(159..161),aa:Ser,seq:gct)
     gene            274..1692
                     /locus_tag="PKG74_02555"
     CDS             274..1692
                     /locus_tag="PKG74_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOB90185.1"
                     /translation="MPENKENLEKRSPVVVVLGHVDHGKTTLLDWIRKTKTAEKEAGG
                     ITQSIGAYEIIHNGERITFLDTPGHEAFKAMRLRGAKVADMAILVVASDEGLKPQTEE
                     SIRILKETKTPYVIAFTKTDLPTANIEKIQNDLTAKEVLLEGRGGDVSWQAVSGKTGE
                     GVNELLDLVLLLAEFLDLTFDPGIDARGFVIECRKDPRQGNIATVIVKNGVLKPGIVI
                     KTLTADGKIKILEDYAGRTAEGLKPSSPALVIGFNNLPQIGEEFVVGEGKLTPLIAEA
                     TVLAEKEKQELKPAVLKADVSGSLEVLKDIFSDKVNILESSAGEITDGDIKTAISGKA
                     FIIAFRTKMNRLAEGYAKNQQVKIIDSDIIYDLIEQVGDYLDTFKEEEASGKLKVLKI
                     FGKSGSEQTIGGEVVEGAIQNNRRITVSRNNIELGEGRIINLQENKVDVQEVKAGSQG
                     GLLVESSIVIKPGDEISQRAVK"
     gene            1664..1993
                     /gene="rbfA"
                     /locus_tag="PKG74_02560"
     CDS             1664..1993
                     /gene="rbfA"
                     /locus_tag="PKG74_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOB90186.1"
                     /translation="MKFRKERLNSVLRDELSKMIQKEIEFPAGVLATITEVDTAPDFS
                     ESKVGVAVLPSDKSNEVLKILNEQAGHFYSLIFKKLKIYSVPKIKFIYDPGSENAARV
                     EEISLKQ"
     gene            2009..2083
                     /locus_tag="PKG74_02565"
     tRNA            2009..2083
                     /locus_tag="PKG74_02565"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2041..2043,aa:Cys,seq:gca)
     gene            2085..2171
                     /locus_tag="PKG74_02570"
     tRNA            2085..2171
                     /locus_tag="PKG74_02570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2118..2120,aa:Leu,seq:taa)
BASE COUNT          671 a          525 c          600 g          535 t
ORIGIN      
        1 gataaagccg gcagcgatga aaaagatata gaaaaaataa gagaattcgt tagaatcgcc
       61 aaatcttctt aagaagataa gtatctatta tatagttcga gcaaacggcg gagaggcagg
      121 gattcgaacc ctggaagccc ttgcgagctt aacacgttag caccgtgtcc cattagaccg
      181 ctctggcacc tctccttgaa cgcaaaagca aaacgtgatt ttgctttatt ttcgcttgtt
      241 tttataccat ttttctgcta attttacaaa gcgatgccgg aaaataaaga aaatttggaa
      301 aagagatcgc ccgtagtcgt ggtcttggga cacgtcgacc acggcaaaac cacgcttttg
      361 gactggatcc gcaaaaccaa gaccgccgaa aaagaagccg gaggtattac ccaatccatt
      421 ggcgcctacg aaatcatcca taacggcgaa agaatcactt ttttggacac gccgggacac
      481 gaagctttta aggcaatgcg gcttaggggc gcgaaagtgg ccgatatggc cattttggtc
      541 gtggcgagcg acgaaggatt gaaaccccaa accgaagaat cgatcaggat tttgaaagaa
      601 acaaaaactc cttatgttat cgctttcacg aagaccgacc ttccgacggc caatatcgaa
      661 aagatccaaa acgatttgac ggccaaagag gttctgctcg agggccgggg cggagacgtg
      721 agttggcagg cggtttcggg caaaaccggc gaaggcgtga acgagctttt ggatttggtg
      781 ttgcttttgg ccgagttctt ggatttgact tttgatcccg gaatcgatgc ccggggattt
      841 gtgatcgaat gccgtaaaga tccgagacag ggaaatatcg cgacggtcat cgtcaaaaac
      901 ggcgttttga agccgggaat cgttatcaag actttgacgg ccgacggaaa aataaaaata
      961 ctggaggatt atgccggccg gacagcggaa ggcctcaagc cctcaagccc ggctttggtg
     1021 atcggtttca acaaccttcc gcagatcggc gaagaattcg tcgtcgggga aggaaaattg
     1081 acgcctttga tcgcggaagc gactgttttg gcggaaaaag aaaaacagga attgaaaccg
     1141 gcggtgctca aagctgatgt ctccggatcg ctcgaggttc tgaaagatat tttcagcgac
     1201 aaagtgaaca ttcttgagtc ttcggccgga gaaatcaccg acggcgacat caagaccgcc
     1261 atttccggaa aagcctttat catcgctttc aggacaaaga tgaaccgttt ggccgaagga
     1321 tacgccaaga accagcaagt aaagattata gattcggata ttatctacga tctgatcgag
     1381 caggtaggcg actatctcga tactttcaag gaagaagagg cgagtggaaa actaaaggtt
     1441 ctgaaaatct tcggcaaaag cggcagcgag cagaccatcg gcggagaagt cgtcgagggc
     1501 gcgatccaaa acaatcgccg aatcactgtt tcccggaaca acatcgagct cggcgaaggc
     1561 cgaatcatca atcttcagga aaataaagtc gatgtccagg aagtgaaagc cggcagccaa
     1621 ggcggacttc tcgtcgaatc atcgatcgtt atcaagcccg gagatgaaat ttcgcaaaga
     1681 gcggttaaat agcgtccttc gggacgagct ttcgaagatg atccaaaagg agatcgaatt
     1741 tccggccggg gttttggcga cgatcaccga agtcgacact gcgccggact ttagcgaatc
     1801 caaagtcggc gtggccgttt taccgtcgga taaatcaaac gaagttttga aaatattgaa
     1861 cgaacaggcc ggtcattttt acagccttat tttcaagaag ctcaagattt acagcgttcc
     1921 gaaaataaag tttatttacg accccggatc ggaaaatgcc gccagggttg aggaaatttc
     1981 cttaaaacaa tagaataaaa tcagtttcgg cgtcgtagcc aagtggtaag gcaagggtct
     2041 gcaaaaccct tatacgtggg ttcgattccc gccgacgcct ccaggcccag gtgctggaat
     2101 ggtagacagc caacacttaa aatgttgtga ggtttatcct cgtgtgggtt cgagtcccac
     2161 cctgggcacc agtattaggc ttttaggaat ttcgttcgcc ccccccgctt tccgccgccg
     2221 agtttcttta tctttgcaag ttcaaatatt agagacacat tgttccgtcg tccgaagagg
     2281 gtggaatggt attcttaatt tctattcccg aaattggttc ataatcttcc a
//
LOCUS       DAOPQT010000090         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_50427_length_2311_cov_3.421986, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000090 DAOPQT010000000
VERSION     DAOPQT010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50427_length_2311_cov_3.421986"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PKG74_02575"
     CDS             complement(<1..284)
                     /locus_tag="PKG74_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HOB90187.1"
                     /translation="MKSEKKSKETKLSNKEDQSKALDALVESLREKYGEESIMKLGET
                     KRADVEVIASGSFSLDRALGVGGFPKGRIVEIFGPEMSGKSTLGLQVIAEA"
     gene            complement(241..912)
                     /locus_tag="PKG74_02580"
     CDS             complement(241..912)
                     /locus_tag="PKG74_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOB90188.1"
                     /translation="MDNEKYNEKTLAELMEELMAGQGLNIDKLGEATNIPKRYLLALM
                     ENDSKNLPAEPYVRGYLLKIAEVAGVDPTGLYSAYKKLGVKTSGKEDSLPSNRFAIKK
                     PASRWLIPAVLVLCLVIAVSFKIKDILGIPSIEVNLPETALIVYAPMAGIEGRIDPKD
                     SLELNGEPIYPDSTGAFSEQVPLEQGINTFEFKIKRFLGRETKIQRQIIYEIGEEIQG
                     DEIIE"
     gene            complement(905..>2311)
                     /locus_tag="PKG74_02585"
     CDS             complement(905..>2311)
                     /locus_tag="PKG74_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOB90189.1"
                     /translation="VPPPLDLLNSQNEKPTAGDLRANANIIRRTLESFGIPVEMGEIT
                     IGPKVTRYTLKPAEGVKLSRITALNQDLALALAAHPLRIEAPIPGKSLVGIEVPNKSA
                     AIVRLGSLMAYPEFGSSSILTFALGREISGDPVMPNIAKMPHLLIAGATGSGKSVVIH
                     SLIVSLLYKNSPETLKFIMIDPKRVELTAYNKLPHLMAPVITEGKKAVAALRWAISEM
                     DHRYDTLMQSGNRDIQSYNAVHKDEPLNYIVIVIDELADLMAAYGREIESSIVRLAQM
                     ARATGIHLVVSTQRPSVEVITGLIKANITTRIALQVASQVDSRTILDMAGAEKLLGGG
                     DMLFMTSDLSKPKRIQGTYITEGEIKAVAKFISENNEPEMRAIVDDSGSNPGAAVFDF
                     GGENDTDDELYPDALEVVTEAKKASASLLQRRLKIGYARAARLLDIMEDKGIIGPGDG
                     AKPREVYLDQIEHHEDNG"
BASE COUNT          483 a          622 c          595 g          611 t
ORIGIN      
        1 gcttcggcga tgacctggag cccgagcgtg cttttaccgg acatttccgg cccgaaaatc
       61 tcgacgattc ttcctttggg gaaaccaccg acacccaagg ctcgatccaa agaaaacgat
      121 ccgctcgcga tcacctcgac gtcggctctc ttggtctcgc cgagcttcat gatcgattct
      181 tcgccgtatt tttcccgaag cgactcgacg agcgcgtcga gggctttaga ctgatcttct
      241 ttattcgata atttcgtctc cttggatttc ttctccgatt tcatagataa tctgtctttg
      301 tatttttgtt tcccgaccga gaaacctttt tattttgaat tcgaaagtgt tgattccctg
      361 ttcgagcggc acctgctcgc tgaaagcgcc ggtcgaatcc ggatagatcg gttcgccgtt
      421 caattcgagg gaatccttgg gatcgatccg gccttcgatc cctgccatgg gcgcatagac
      481 gatcaaggcg gtctccggaa gattgacctc gatgctcggt atccccaaaa tatcctttat
      541 cttgaaagaa acagcgataa ccaaacaaag taccaatacc gccggaatca accagcggct
      601 cgccggtttt ttgatggcga atcggttcga cggcaaagaa tcttctttac ccgaagtctt
      661 aacccctagt ttcttgtagg cgctgtagag cccggtcggg tcgactccgg ctacttcggc
      721 gattttcagc aaataaccgc ggacataagg ctcggccggc agatttttgg agtcattctc
      781 catgagggca aggaggtatc ttttggggat attggtcgct tcgcccagct tgtcgatatt
      841 gagtccctgg ccggccatca attcttccat taattcggcc aaggtttttt cgttgtattt
      901 ttcgttatcc attatcttcg tggtgttcga tctggtcgag atatacttct ctcggtttgg
      961 ctccgtctcc agggccgata atgcccttgt cctccatgat atcgaggagc cgcgcagccc
     1021 gagcataacc gattttcagc cttcgctgca agagcgaggc cgaggctttt ttggcttcgg
     1081 tcacgacctc cagggcgtcc ggataaagct cgtcgtccgt gtcgttttct cccccgaaat
     1141 cgaaaaccgc cgccccggga ttggaaccgg aatcgtcgac aatagcccgc atttccggct
     1201 cattgttttc ggagatgaat ttggccacag ccttgatctc gccttcggta atataggtgc
     1261 cttggattct tttcggcttc gataaatccg aggtcataaa aagcatgtct ccgcctccca
     1321 aaagtttttc cgctccggcc atgtcgagga tggtccgcga atcaacctga ctcgccacct
     1381 gaagggcgat cctcgtcgtg atattcgcct tgataagccc ggtgatgacc tcgaccgaag
     1441 gccgctgggt ggagacgacg agatgaatac cggtcgccct cgccatctgg gcgagcctca
     1501 cgatcgaact ttctatttcc cggccgtaag cggccataag atcggccaat tcgtcgatga
     1561 cgatgacgat gtaattcagg ggttcgtcct tatggacggc gttgtagctt tggatgtcgc
     1621 ggttgccgct ttgcatcaga gtatcgtagc ggtgatccat ttcggaaata gcccaacgga
     1681 gcgcggccac ggctttcttg ccttcggtga tgaccggcgc catgagatgg gggagtttgt
     1741 tataggcggt gagctcgacc cgtttcggat cgatcatgat gaatttcaaa gtttccggag
     1801 agtttttgta aagcaaagaa acaatcaagc tgtgaatgac gacggatttt cccgaacccg
     1861 tggctccggc gatgagcaaa tgcggcattt tggcaatatt cggcataacc ggatccccgg
     1921 aaatttccct tcccaaagcg aaagtcaaaa tactcgagct tccgaattcc ggataagcca
     1981 tgaggcttcc cagtcggaca atggccgcgg atttgttcgg tacttcgatg ccgaccaaag
     2041 acttacccgg aatgggcgct tcgatcctca aaggatgggc cgccaaagcc aaagccaaat
     2101 cttggtttaa ggcagtgatc ctcgaaagct ttacgccttc ggccggtttc aaagtgtatc
     2161 ttgtcacctt ggggccgatg gtgatttctc ccatttcgac cggtattccg aaactctcga
     2221 gcgtccgcct gatgatgttg gcgttcgccc gcaaatctcc ggccgtgggc ttttcgtttt
     2281 ggctgttcaa aagatcgagc ggcggcggaa c
//
LOCUS       DAOPQT010000091         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_50460_length_2310_cov_2.624390, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000091 DAOPQT010000000
VERSION     DAOPQT010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50460_length_2310_cov_2.624390"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PKG74_02590"
     CDS             <1..980
                     /locus_tag="PKG74_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOB90190.1"
                     /translation="DEIPNLLNDPKSPILNRPVMGKYSPASTVKPFLSVAALKEGVIK
                     PSDLVYSSGVLEVPNPYYPDQPTLFPDWKPHGWVDLYAAIARSSNIFFYAVGGGLPYN
                     EDLIQGETDFKGLGMPLIEKYLKLFGFNEKTGVEIPGEATGFIPSPSTQKERTGIAWR
                     LGDTYQVSIGHGDLKISLLELIRAYAAIVNNGLVYKPHIVAGSEPEIVRDISYLEPEM
                     QTTLKGMSDAIYKSYGTCTSLADLDYKSVAKTGSASVLDNKKINAIFVGCGPIPIEDY
                     PSICVLVLAENAKTSTTNAVPIGRDVLDWYYKNRVGRSVDSATGDVSAE"
     gene            1012..1575
                     /locus_tag="PKG74_02595"
     CDS             1012..1575
                     /locus_tag="PKG74_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90191.1"
                     /translation="MSKSLKIILIVLLVLAVGIFVAWREGLFTSFRLFSSGMSKPGTC
                     LLVEERYCSTAKFIYFEEARDPILGFKIPAGAPVFAPTDGSATPIFMDNPTNPNSPYS
                     GILLRHFVIPNSTSGLITVNVIANPLSDANQNPEVQTGDLLGYVSENTVDVFGDYNLI
                     VNIENQALFPGTVYSNTNLYIKDKLGL"
     gene            1666..>2310
                     /locus_tag="PKG74_02600"
     CDS             1666..>2310
                     /locus_tag="PKG74_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90192.1"
                     /translation="MKKILFFTFLISGFFLISFRTAKAQCFEGDFPPDSYFQCVNGQI
                     CQGNECVSYDEVPRTYLPSDQVLDSQEEEIWYKRQWTLWQDTGGQTGCHPYTTCSTSY
                     SVDSTNGCPGSSCGQKSSELGLSTANLNPWGKYQIEECKSSFIGNDTFFRPVSYYLYR
                     ITSRLYSTTDKCTNIDSSNQCLAGEENKGLWTGSCEGSVGDFYKCCCHNQTMESV"
BASE COUNT          593 a          548 c          550 g          619 t
ORIGIN      
        1 tcgacgaaat acccaatctc ttgaacgatc cgaagagtcc gatcctcaac cggccggtca
       61 tgggaaaata cagcccggct tcgaccgtga aacctttctt gtccgtcgcc gccctgaaag
      121 aaggagtcat caaaccgagc gatcttgttt attcttcggg agtgctcgag gtgccgaatc
      181 cgtattatcc ggatcagccg accctttttc cggattggaa gccgcacggt tgggtcgatc
      241 tttatgccgc tatcgcacgc tcgagcaaca tttttttcta cgccgtggga ggcggcttgc
      301 cgtataatga agatttgatc cagggcgaaa ccgatttcaa gggactgggt atgcctttga
      361 tcgagaaata tttgaagctt ttcggattca acgagaaaac cggcgtggag attccgggag
      421 aggcgaccgg attcattcct tctccttcga cccagaaaga aagaaccggc atcgcctgga
      481 gattgggaga cacttaccag gtttccatcg gacacggaga cttgaaaatc agtcttctcg
      541 agcttatccg ggcctacgcc gcgatcgtta acaacggctt ggtctacaaa ccgcatatcg
      601 ttgccggaag cgagccggag atagtccgcg atatttctta tctcgagccg gaaatgcaga
      661 caacattgaa aggaatgtcg gacgcgattt acaaatctta cggcacctgc accagtttgg
      721 cggatctcga ttacaagagc gtcgccaaaa ccggaagcgc ctcggttttg gacaacaaga
      781 aaattaacgc catttttgtc ggctgcgggc cgataccgat cgaggactat ccgtcgatct
      841 gcgttttggt gctcgcggaa aatgccaaaa ccagcacgac gaacgccgtt ccgataggcc
      901 gcgatgtgct cgattggtat tataagaacc gggtgggaag gtccgtggac tcggcaacgg
      961 gagacgtttc agcggaatga tcaggacgtt atatgttata ctgaaattat tatgtccaaa
     1021 tccttaaaaa taattctaat cgttctcctt gttctcgcgg tcggtatttt cgtcgcctgg
     1081 agagaagggt tattcacttc gttcaggctc ttttcttcgg gaatgtcgaa gccgggaacc
     1141 tgcttgttgg tcgaagaaag atactgctcc acggccaagt ttatttattt tgaagaagcg
     1201 agggatccga ttctgggatt taagattccg gcgggagcgc cggttttcgc tccgacggac
     1261 ggatcggcaa cgccgatatt catggataac ccgaccaatc ccaacagtcc ttattccggt
     1321 attttactgc gtcattttgt catcccgaat tcgacctccg gcctcattac cgtcaacgtt
     1381 atcgcgaatc ctttatccga tgcgaatcaa aaccccgaag tgcagacagg ggatcttttg
     1441 ggatatgttt cggaaaacac ggttgatgtt ttcggagatt ataacctgat cgtgaatatc
     1501 gagaaccaag ccctgttccc gggcacggtt tattcgaata ccaatcttta cattaaagac
     1561 aaattggggc tttaagctcg tcaaaacttt accccaatct ttttttgata atccttattt
     1621 ctttgaaaac gagggttatc gtttgtctat gatataatta tttcaatgaa aaagattctc
     1681 tttttcacct ttttgatatc tggatttttt ttgataagct tccgcaccgc taaagcccaa
     1741 tgctttgaag gagattttcc tccggacagc tatttccaat gcgtgaacgg tcaaatctgc
     1801 caaggaaacg aatgcgtttc ctatgacgaa gttccgagaa cataccttcc ttcagatcag
     1861 gtgctcgatt cgcaagaaga agaaatatgg tataaaaggc agtggacgct gtggcaagat
     1921 accggagggc agacaggctg tcatccctat accacttgct caacatcgta tagcgtagac
     1981 agcaccaatg gatgtccggg gtcttcttgc ggccaaaaat caagcgagtt gggtctgtcg
     2041 accgccaact tgaatccttg gggtaaatat caaatcgagg agtgcaaatc ttcgtttata
     2101 ggaaacgaca cattttttcg tcccgtatct tattatcttt accgtatcac ttctcgtctc
     2161 tattccacta ccgacaaatg cactaatata gattcttcca atcaatgtct tgctggcgaa
     2221 gagaacaaag gcctttggac cggttcatgc gaaggaagtg tcggggattt ctataaatgc
     2281 tgctgccaca atcaaacaat ggaatctgtt
//
LOCUS       DAOPQT010000092         2295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_50876_length_2295_cov_3.561607, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000092 DAOPQT010000000
VERSION     DAOPQT010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50876_length_2295_cov_3.561607"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1350
                     /locus_tag="PKG74_02605"
     CDS             217..1350
                     /locus_tag="PKG74_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90193.1"
                     /translation="MEDWESLLSEAESIIEITARRFDNIDGYDYEDLLQIGRIKVWEI
                     LSEKKGTFAAMKGYIAVSLANLFRTEFGKTKAAKRINLRQTTSLDAKIDDDDDRTRYD
                     FIPDKGGAITLETLDIVKTAALKTKAKRAIRGVVWCLMELLGIRTEQAPKKINYGTFV
                     KYGLSRYLWVFFNNSPYRALRYAYPELKAKDMKKRPNNYWKGERGKRKAVEELREILL
                     SSGYGERDYPLVVNHDFIVDHGLSFPLQTMYGSSPFLFLDAVFPGRFHPWEMATTPKY
                     WFESDDRAIAATKWLVEEVLGFDIPNLDEKEIWRRRIGVKIRRSDFDDNGLRGLISQF
                     GNSPKRLLELAYPGKFQEWDFQSKDKNGSVPPDWNSPPAPRGG"
     gene            complement(1537..1881)
                     /locus_tag="PKG74_02610"
     CDS             complement(1537..1881)
                     /locus_tag="PKG74_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOB90194.1"
                     /translation="MKTFINWVISAVAIIIAAYVLPGVNVDGFLAVLVLAVVLGAINA
                     FIKPIILFLTFPINIVTLGLFTLVINAALVILASYIVPGFSVASFWWAMLFSIVLSFV
                     TTVFSSFGTDNK"
     gene            complement(1954..2024)
                     /locus_tag="PKG74_02615"
     tRNA            complement(1954..2024)
                     /locus_tag="PKG74_02615"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1990..1992),aa:Gln,seq:ttg)
BASE COUNT          663 a          484 c          552 g          596 t
ORIGIN      
        1 ggcttcctcg gccgccgcct tgatggcttt tttcaattta tccttgtcga acttttcggt
       61 cccaccgtgc ctttttacaa cttctttagt cattgtgaaa ataatttacg acctttggca
      121 acggaaagat tgctgctgat tcagtatagc ataatcgttt gacctcggtt ttgataaaag
      181 ataacattcg accagttcct tgaaaggaga atatctatgg aagattggga atcgcttctt
      241 tccgaagcgg aaagtataat cgagataacg gctcgtagat tcgacaatat agacggttat
      301 gactacgaag atctccttca gatcggaagg attaaagtct gggagatatt gtcggagaag
      361 aaaggaacat tcgccgcaat gaagggctat atagccgttt cgctcgccaa ccttttccgt
      421 accgaattcg ggaaaaccaa agcggcaaag aggatcaatt tgcgtcagac gacatctctt
      481 gacgcgaaaa tcgacgatga cgatgatcgc acccggtatg attttatacc cgataaggga
      541 ggggcgatta cccttgaaac gctggatatc gttaaaactg ccgctttgaa gacaaaggca
      601 aaaagagcga tacggggagt ggtctggtgc ctgatggagc ttctcggtat tcggacggag
      661 caagctccga aaaagataaa ctacggcact tttgtgaagt atggactttc ccgttatctt
      721 tgggttttct ttaataactc tccttaccgc gccttgcgtt atgcttatcc ggaactcaag
      781 gcgaaggaca tgaagaagag gccgaataat tattggaaag gagaacgagg caaaaggaag
      841 gccgtagagg agctgagaga gatactcttg tcttcgggat acggagagag agactatccg
      901 ttggtcgtga atcacgattt tatcgtcgat cacgggctgt cttttccgtt acagaccatg
      961 tacggctcaa gcccatttct ttttctcgat gccgtttttc ccgggagatt tcatccgtgg
     1021 gaaatggcga ccactccgaa gtattggttc gagagcgacg atcgtgcgat cgccgcgaca
     1081 aaatggcttg tcgaagaagt tttgggattc gatatcccga acttggacga gaaggaaatc
     1141 tggagaaggc gaataggcgt caagataaga cgaagcgatt tcgacgataa cggattaaga
     1201 ggcctgatca gccagttcgg taattctccg aagagactgc tcgaactggc ctatccaggt
     1261 aagtttcagg aatgggactt ccaaagcaag gataaaaatg ggtcggtccc gccggattgg
     1321 aactcgccgc ccgcgccacg aggtgggtaa tcgaagaata tgccggtctc actccgtggt
     1381 cctccggaat cggattcaga ttcttcatag agaacggttt gcacggcatg atcaccgcca
     1441 aaagcctggg gttcaattca tcgcccagag cggcgttgat gaatgcttac ccgcaaaatg
     1501 tatgactcct ccttacgagg agtctttcta tattaattac ttattgtctg tgccaaacga
     1561 cgagaaaacg gttgtcacaa aagacaaaac aatgctgaac agcatcgccc accagaaact
     1621 ggcgacgctg aatcccggaa caatgtagga agcgagaatg acgagtgcgg cgttaataac
     1681 caaggtaaaa agcccgagag ttacgatatt gatcggaaaa gtgagaaaga ggattatcgg
     1741 cttgatgaaa gcattgatcg ctccgagcac aaccgcgaga acgagcacgg ccaaaaagcc
     1801 gtcaacgttc actccgggca aaacataggc tgctattata atggcgacag ccgatataac
     1861 ccaatttata aaagtcttca taataatgaa ttaatttgat tggtttataa taagataatt
     1921 atagcataat tatcttatta tggatctgtt ttgctgcggg gctaggaatc gaacctagag
     1981 ttcctgcttc aaaggcaggc gtgttaccat tgcaccaccc cgcaataatt actgaaaata
     2041 ctctatcata gccttaataa gtcctgcaat tttacggttt aatctagggt tttttaacct
     2101 taattcttaa cactccaggg taatcatttt tatttctcaa tcagctataa tgtttatgtt
     2161 attatggcgt tatgactatc gaatacgaag cgactttccc gaatatcgat aagaccgaat
     2221 tccgcgatcg attgattaaa gtcgaagcca agcttttacg gtcggaattc ttgcaaaaaa
     2281 gaaaggtttt tcatt
//
LOCUS       DAOPQT010000093         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_51981_length_2257_cov_3.599001, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000093 DAOPQT010000000
VERSION     DAOPQT010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51981_length_2257_cov_3.599001"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PKG74_02620"
     CDS             complement(<1..1376)
                     /locus_tag="PKG74_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOB90195.1"
                     /translation="MNKTIAKNSFLMLLGQSVGRLLRAGIIIYAARVLGADSWGSLEN
                     ALGISALFVIFADLGINSLLIREANRKPEEQKAYLATSLAIKTSLVAILAVLMNVFAG
                     KLSNLPGAIALMPIVALVFIFDSLRDLGVSLSRSLERMEIEAVVNVLTNLFIVILGFM
                     ALRQFGTNQSFALAYAIGAGLGTIAVFIPLKKYFQGIWKSFDKKLVKPIIVSAWPFGL
                     VSLLGIVMINIDMIILGLISTAEQVGFYSAAQKPIQFIYLIPSLISAAFLPSLSRLAC
                     KNDKKDCFRDRIEQGIKATLLIALPAALGGVILAEPIMLLLYGPEYLAGTMSFLVLCL
                     TFVVVFPSALIVNAAFAAEKNQKEFIIYAFLGIAGKIVLDLILIPRLGIIGAAISTFL
                     NQIIINTYIWRKMKQMADFRVLPQLRKIVPAAGIMGLVSYALLQLGVPVILNIAVAVI
                     VYIATLWLL"
     gene            1614..1937
                     /locus_tag="PKG74_02625"
     CDS             1614..1937
                     /locus_tag="PKG74_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90196.1"
                     /translation="MSRIRRGWVLFFAAIVIAKEGIPSAEDAKRRIREIPRKRGIFVY
                     PEKEMYNKSDMNAALIFFGTWAVVSPFFTGNVGTSIFYSNILLGLALVLTACWRLCGC
                     RGKKK"
     gene            1959..2192
                     /locus_tag="PKG74_02630"
     CDS             1959..2192
                     /locus_tag="PKG74_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOB90197.1"
                     /translation="MKVKIYSTPVCVYCRMAKEFFKKNNVEFEEVNVAVDRQAAEEMV
                     AKSHQMGVPVIDIDGEIFLGFNRRDLEEKLGIR"
BASE COUNT          627 a          514 c          606 g          510 t
ORIGIN      
        1 agcagccaga gagtagcgat ataaacgatt acggcaacgg cgatattcaa tatcaccgga
       61 acgccgagtt ggagcagggc gtaagagaca agccccatga ttccggccgc cggaacgatc
      121 tttctaagct gcggtaaaac ccggaaatcc gccatttgct tcatttttcg ccagatatag
      181 gtattgatga tgatttggtt caggaaggta gaaatagccg ctccgataat ccccagtctc
      241 ggaatgagaa taagatcgag gacgattttc ccggcgattc cgagaaaagc gtagataatg
      301 aattctttct ggtttttctc ggcggcaaag gcggcgttca cgatcagggc cgagggaaaa
      361 acaacgacaa aggtaagaca gagaacgagg aagctcatcg ttccggcgag atattccggg
      421 ccgtataaaa gaagcatgat cggttcggcg agaataacgc cgcccaaggc cgccggaagg
      481 gcaatgagca aggtcgcctt tataccttgt tctatccgat cgcggaagca gtctttttta
      541 tcgtttttgc aagccaagcg cgacagactc ggcaggaaag cggcggaaat aaggctcggg
      601 ataaggtaga tgaactggat cggtttttgg gcagccgaat aaaacccgac ttgttcggcc
      661 gtggaaatga gccccaagat tatcatgtcg atattgatca tgacgatccc taagaggctt
      721 acgaggccaa aaggccaggc ggaaacaatg atcggtttga cgagtttctt gtcgaagctt
      781 ttccagatcc cttgaaaata tttcttgaga ggaatgaaaa cggcgattgt cccgagtccg
      841 gcgccgatcg cgtaagccag ggcaaagctc tggttggtgc cgaactgccg caaagccata
      901 aatccgagga taacgatgaa aagattggta aggacgttca ccacggcctc gatctccatt
      961 ctctcgaggc tgcggctcaa ggaaactccg agatccctca aggaatcgaa aatgaaaact
     1021 aaagcgacga tcggcatcaa agcgatcgct cccggaaggt tgcttagttt tccggcgaaa
     1081 acattcatca aaaccgcgag aatggccacc aaactggttt tgatagccaa ggaggtggcg
     1141 agataggctt tttgttcttc cggctttcga ttggcttcgc ggataagcag ggaattgatg
     1201 ccgagatcgg cgaaaatgac gaaaagcgcg gaaatgccca gggcgttttc gagagatccc
     1261 caggaatcgg cccccaaaac ccgggcagcg tagatgatga tcccggcccg caaaagccgg
     1321 cccacgcttt ggccgagaag catcaaaaag gagtttttgg cgatggtctt gttcataaag
     1381 tccctttatt ttgcctttaa tttgacaaaa agtccaaaat caccgataat cctaaacaat
     1441 tagtttagag acagatcagg cagttttccc aaaacgttaa gccattgtgt ttcgactaag
     1501 aaaaggtcaa aagtttaaac caataatggc ttataacgat aacgctccga ggcagatgtt
     1561 caagggcgat tggaagtgct ccaagtgcgg cgctccgatc accgaacttc cctttgagcc
     1621 ggatccgtcg aggttgggtt ctcttctttg ccgcgattgt catcgcgaaa gaaggaattc
     1681 cttcggcgga agacgctaag cgtcgcatcc gagaaatccc gcggaaacgc gggatttttg
     1741 tatacccgga aaaggaaatg tataataaaa gcgacatgaa cgccgctttg atatttttcg
     1801 gaacctgggc cgttgtttct ccgtttttca cgggaaacgt cggaacgagc attttttaca
     1861 gcaacattct gctcggattg gcgcttgtcc ttacggcctg ctggaggctt tgcggctgcc
     1921 gtggcaagaa gaaatgattt tgtgctaata tcatcacgat gaaagtaaaa atatattcga
     1981 ctccggtttg cgtctactgc cgcatggcca aggagttttt caagaaaaac aacgtcgaat
     2041 tcgaagaagt gaatgtggcc gtcgacagac aggcggccga ggaaatggtc gcgaaatccc
     2101 accaaatggg cgtgccggtg attgatatcg acggagaaat ctttttggga ttcaaccgcc
     2161 gcgatttgga agaaaaactg ggaataagat aagttctctt atgtacgagc ttgtcatcat
     2221 cggcggagga ccggcgggaa tggccgccgg catctac
//
LOCUS       DAOPQT010000094         2247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_52289_length_2247_cov_4.722172, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000094 DAOPQT010000000
VERSION     DAOPQT010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52289_length_2247_cov_4.722172"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PKG74_02635"
     CDS             complement(<1..430)
                     /locus_tag="PKG74_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HOB90198.1"
                     /translation="MKDIIVIDIETKNTFADVGGDEHIDELEVSVACVYSYNENKFRS
                     FWETNMEELGNLLKESGLVVGFSINRFDLPVLDRYFPFSTKALKRLDLLEEIELSFGS
                     RIGLDLLAQTNIGLCKTHHSLEAIRLWNEGQHKELENYCQN"
     gene            complement(525..1031)
                     /locus_tag="PKG74_02640"
     CDS             complement(525..1031)
                     /locus_tag="PKG74_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90199.1"
                     /translation="MSVTSDISPDRLFLESILDIVKHNKVTVSVDEAFWEEGDFYLVG
                     QDFEQDNFIKLPSREFLFLLQDLADVAALFDEPVFIIEGKIPEESMLSERDCIIRKRA
                     IIALWRISSEIPAGQIGLVIHEAEGKAWIGRYRLQKFREIMAKASLSYRLITCEDAQR
                     ICCYRHEL"
     gene            complement(1278..1607)
                     /locus_tag="PKG74_02645"
     CDS             complement(1278..1607)
                     /locus_tag="PKG74_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90200.1"
                     /translation="MKKFEALLNSVVEADKRDQEAKKEASEAARAAYFAAYRAADASI
                     SGSVKITLPNGGWISVFRGGAVITSSSRAVCAAEVLMNKDVGRFFAAAVAAAEKDELQ
                     ISIPAFS"
     gene            1724..>2247
                     /locus_tag="PKG74_02650"
     CDS             1724..>2247
                     /locus_tag="PKG74_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90201.1"
                     /translation="MSKNKHRKQKPVEPKPNRLTLSPEVKRSIVGVAFCGLSAILFLA
                     SRAKAGPVGDFLFHNLSIAFGVGYYFIPTIFAILGIGFLTSGEKREFGQNAFFGAVLF
                     VIAGLSLLDILYPEKGGAAGNIFGAIEIPFGKIAGIIISVSVFIISVIITLDIPLKFS
                     FLKKKSEIGTIKPV"
BASE COUNT          604 a          549 c          480 g          614 t
ORIGIN      
        1 cgttctggca ataattctcg agttctttgt gctggccttc gttccaaagc ctgatggcct
       61 ccaaactgtg atgggttttg caaagcccga tgttcgtctg ggccaagaga tcgagtccga
      121 tgcggctgcc gaaactcaac tcgatttcct cgagaagatc gaggcgcttc aaagccttgg
      181 tggaaaaagg aaaatagcgg tccagaaccg gcagatcgaa gcggttgatg ctgaatccga
      241 caaccaatcc cgattccttg agcaggtttc cgagctcttc catgttcgtt tcccaaaagc
      301 tccggaactt gttttcgttg taggaataga cgcaggccac ggaaacttcg agttcgtcaa
      361 tgtgctcgtc gccgccgacg tcggcgaagg tgtttttggt ttcgatatcg ataacaataa
      421 tgtctttcat agatttaaga tattgaagaa taacgggttt ttcggaaaaa ttcaatcttt
      481 tgaataaaaa accccgtaaa cgaatacagg ggacttcgat tccatcaaag ttcatgacga
      541 taacaacaaa tcctctgagc gtcttcacaa gtgatcagcc tataagacaa agatgctttg
      601 gccattattt cccgaaactt ttgaagccgg taacggccga tccaagcctt tccctccgcc
      661 tcgtgaatga caaggcctat ttgaccggcc ggaatctcgg agctgattct ccaaagcgct
      721 ataatcgccc tcttgcggat aatgcaatcc cgttcagaca acatagactc ttcgggaatt
      781 ttgccctcga tgatgaaaac tggttcgtcg aacaaagcag cgacatccgc caagtcttgt
      841 agcagaaaaa ggaactctct tgacggaagt ttgatgaaat tatcttgttc gaaatcctgg
      901 cccacaagat aaaaatcacc ttcttcccaa aacgcttcat ccacggacac ggtaaccttg
      961 ttgtgtttga cgatgtctaa aatgctctcc aggaacaacc tatcgggact tatatccgaa
     1021 gttacgctca ttattcttcc tccttaaaga ccattcttat ggatagaagt aacatgcccc
     1081 taacgggttg tcaaaaacag attctaataa aaactatccc gcggattaac gcaggataat
     1141 agtcgacatg tcttaggaac ggggctcgta atagaaaagc cccatctcta tcattttgac
     1201 gaagatgaga ccgtatggtt ttgattttta aaaacaaaac ggcccacaag cattgcttgc
     1261 gggccaaaga acgaaatcta ggaaaaggcg gggatggaga tttgaagctc gtctttctca
     1321 gccgcagcca cagctgcagc aaaaaaacgg cctacgtctt tgttcattaa tacctccgcc
     1381 gcgcaaacgg cgcgagaact agaggtaata actgctcctc ctctaaaaac ggagatccat
     1441 cctccgttag gaagagtgat tttgactgaa ccagaaattg atgcatcggc ggcacggtaa
     1501 gcggcaaagt aagcggctct agccgcttcc gacgcctctt tcttagcctc ttgatccctt
     1561 ttgtccgctt cgacgacgga gttgagtaat gcctcgaact ttttcaatcc ctttacctcc
     1621 ttaaggaact cttattatat tatattttaa ctatagcaca ctttgtccac cttgtcaaga
     1681 ccaattcatt tttcttgatt tttactatat aatgagcctg tttatgtcca agaataagca
     1741 tcgcaagcaa aagccggtcg agcccaagcc aaacagactt actcttagtc cggaggttaa
     1801 aagaagcatt gtcggagtcg ctttttgcgg actctcggct attttattcc tcgcctcaag
     1861 agccaaggcc ggaccggtcg gagattttct tttccataat ctctccattg ctttcggggt
     1921 cggctactac tttattccga cgatcttcgc cattttgggc atcggttttc tcacttccgg
     1981 agaaaaaaga gaattcggtc aaaatgcctt tttcggcgcc gtattgttcg ttattgccgg
     2041 acttagccta cttgatattc tttatccgga aaaaggcgga gccgccggca atattttcgg
     2101 ggcaattgag attcctttcg ggaaaatcgc cgggattatc atttcggtga gcgttttcat
     2161 catcagcgtc atcatcaccc tcgacattcc gctcaagttt tctttcctca agaaaaaatc
     2221 cgaaatcgga acaatcaaac cggtttt
//
LOCUS       DAOPQT010000095         2213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_53395_length_2213_cov_3.322984, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000095 DAOPQT010000000
VERSION     DAOPQT010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53395_length_2213_cov_3.322984"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKG74_02655"
     CDS             <1..347
                     /locus_tag="PKG74_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90202.1"
                     /translation="EFQGRTLVANPAFPIGVIDGIVDNDPDIAGQTIVLRADVLKIYP
                     EAPFDSKLITISVENAEVFFMNNGRNLAPDDIRNIKAGTAISVGLAEGETNRDILFKD
                     EFEAIKITFFDK"
     gene            685..2034
                     /gene="murC"
                     /locus_tag="PKG74_02660"
     CDS             685..2034
                     /gene="murC"
                     /locus_tag="PKG74_02660"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOB90203.1"
                     /translation="MSKKTKFKKTDSYKAVHFVGIGGIGMSSLASWFLAQNWAVSGSD
                     IQDSQILKGLTKKGAKVKIGHKSSNIPANASLLIHSAAIPAQNPEIKEAKVRNMEILT
                     YAQALGRVMNDYKTIAVAGAHGKSTTTAMVAKILIEAGLDPTLILGSKIDGSNFRFGK
                     SDYLIVEADEYDGSFWNYRPWAALVTNIDLEHLDFYKDLGAVKKSFIKFLNQADKDGF
                     IVLNKDNENLVSILPSLKNTHIAWYSIKDKRRKIIKDLLRVPGKHNVSNALGAMTLAL
                     NLGIDEKTIFRALSGFKGIWRRLEYRGETKSGFKVFDDYAHHPTEIKASLQALKEKFP
                     DSNLVCVFQPHQAKRLEALFKDFAKAFNQADHLILLDVYQVVGREEVSQNITSEDLAM
                     AVANVNKIKDISYLPDSPERAEILRITLEEIKEAKKNNVVVMMGAGDIFTLTDKLLK"
BASE COUNT          642 a          542 c          506 g          523 t
ORIGIN      
        1 ttgaattcca aggtagaact ctggtggcca acccggcatt cccgatcggg gttatcgatg
       61 ggatagtcga caacgatccc gatatcgccg gacaaacgat tgttctcagg gccgatgttt
      121 taaagatata tcccgaagcg ccattcgact ccaaacttat cacgatatct gtcgaaaacg
      181 cggaagtgtt ttttatgaat aatggacgaa acctggcgcc cgacgatatt agaaacatca
      241 aagccggtac ggccataagc gtcggtcttg ccgagggaga aaccaatcgg gatattttgt
      301 tcaaagacga attcgaagcg attaaaatca ccttcttcga caaataagag gggagaaggt
      361 tcttgactgc tttcaagctc ccgacattcc gggagctttt ggtaaaaaac aaattgctta
      421 gcttaaagga cgattgtata ctaatggata agttgttttc aaaagtataa ggattgattt
      481 tccggttagc tgccatgacg gggaagagca agctttaagt ctggtgatac gtcgcataat
      541 atacctttta cgacagtcct tctccgggaa acccttgttc ggcccttctc tatatttatt
      601 tttataaaac aaccgattgg tttatacgaa tcatatttct tatcacggtc gctaacgccg
      661 tgccataacc tctttcaagc ttacgtgtct aaaaaaacaa aattcaagaa aaccgattct
      721 tacaaggctg tccacttcgt cggtatcggc ggtatcggca tgagcagcct cgcttcctgg
      781 tttttggccc aaaattgggc tgtaagcggc tcggatatcc aagatagcca aatactcaag
      841 ggattgacta aaaaaggcgc caaggtcaaa attggccata aatcctcgaa tattccggca
      901 aatgccagcc ttttgatcca ttcggcggcc attccggcac aaaacccgga aataaaagaa
      961 gccaaggtta gaaacatgga gattctgacc tacgcccaag ctttgggtcg ggtcatgaac
     1021 gattacaaaa caatcgccgt ggccggagcc cacggtaaaa gcacgaccac ggccatggtc
     1081 gccaaaatcc tcatcgaagc cggactcgat ccgactctga tcttgggaag caaaatcgac
     1141 ggttccaatt tcaggtttgg aaagagcgat taccttatag tcgaggccga cgaatacgac
     1201 ggttcgtttt ggaactaccg tccttgggcg gctcttgtca cgaacatcga tctcgagcat
     1261 ctggattttt acaaagatct cggagccgtc aaaaaatcct tcatcaagtt tttgaaccag
     1321 gccgacaaag acggattcat tgttttgaac aaagacaacg agaatctggt ttccattttg
     1381 ccgtcgctca aaaacaccca catcgcctgg tattcgatca aagacaagcg tcgtaaaata
     1441 ataaaagatc ttttgcgggt tccgggaaag cacaatgtct cgaacgccct gggagcgatg
     1501 actttggctt tgaacctcgg catagacgag aaaactattt tccgggcgct ttccggattc
     1561 aagggcatct ggcggaggct cgaataccgc ggcgaaacca aatccggttt caaggtgttc
     1621 gacgattacg cccaccatcc gaccgagatc aaagcctcgc tccaggcgct caaggaaaaa
     1681 tttcccgatt ccaacctggt ctgcgttttc cagccgcacc aggccaaacg cctggaagcg
     1741 cttttcaagg actttgccaa agcttttaac caggcggatc atttgattct tctcgacgtt
     1801 taccaggttg tcggccgaga agaagtgtcg caaaatatta cttcggaaga cctggccatg
     1861 gccgtcgcga acgttaacaa gatcaaagac ataagctatc ttcccgattc tccggaaaga
     1921 gccgagatcc tcagaatcac cctcgaggaa ataaaagaag ccaagaaaaa caacgtcgtc
     1981 gtgatgatgg gcgccggaga tattttcact ttgacggaca aactattaaa ataaccgaat
     2041 gtactactcg aacatcgatc aattcaaaga agccaacggc tacgggctcg aagacgtctg
     2101 gtatccccgc gtgacgagta tcgtgaacat caaagccaaa ccggcccttt accggtatta
     2161 cgccgagctc gccaatttca acgaaggaga agccatcaaa aagcaatcgg ccg
//
LOCUS       DAOPQT010000096         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_53523_length_2209_cov_3.346797, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000096 DAOPQT010000000
VERSION     DAOPQT010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53523_length_2209_cov_3.346797"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="PKG74_02665"
     CDS             <1..1584
                     /locus_tag="PKG74_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90204.1"
                     /translation="ETNDSYPPLEGACPAGSSVKWRSGASIDPSECQNICKPTLPVDP
                     PLFCDLTQNCRHETLEALGISPHLATTFEHNGVLKSGLTKPECNYLCGDLYVCKDSVP
                     VSVKGKYTNHDDCLVGEGVYCFKKEDLIYEQCLAEGISPGQSAYYYCAAPSYDCSKTS
                     QTFSDSESCENTMHGKCYRVSNDCEAECPPEGLGQYYWCNPAGGCSSGNYLSGEECEQ
                     GDEEKVCYSDFGSCSSACRTSGVISCNLPETGSGDDIAVSLEADPQCVPIRSLNYQST
                     LSWEIGIGNSCGEGEEPSNNRFSCVSCNMPSPSVESGSVAVSADRTTEYGISCTRAAY
                     RCCWEEDEDYSCNCVGEEQERVCETCTRAITICDDEHAASTGIAKTSIRVVPKPVIQS
                     FTADPVDILYTAENRVAESGYNKFSTLSWTSLESQFDFPITTSCVVIRDDGTSEAAGL
                     NQSGSRTVSPSRTTIYTLQCRNSDDEEPETCYSDSETENATVRVFGAGIEEPGPQTYL
                     EGFGKKIGQIINGFLDLPE"
     gene            1636..>2209
                     /locus_tag="PKG74_02670"
     CDS             1636..>2209
                     /locus_tag="PKG74_02670"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOB90205.1"
                     /translation="MKKLIVANWKMNPETIAEARSLLSRSLTVISKNKSIDFLAAVPT
                     PYLGDAAFKKRAKYLAGQNVSWAENGALTGEFSAKMLYSLGVKYAIVGHSERREYFFE
                     NEEIIAKKIQKCFEAGIRPILCVGESTAIRRKGLDLVKIYLKDQLRQIIAGTEHSLIS
                     KIVFVYEPVWAVGAEEADDPESSAELINHMK"
BASE COUNT          584 a          545 c          569 g          511 t
ORIGIN      
        1 gaaacgaatg acagctatcc acccttggaa ggagcttgtc cggccggaag ttcggtcaaa
       61 tggagatccg gagcttcgat cgatccgagc gaatgccaaa atatctgcaa gccgaccctt
      121 ccggtcgacc ctcctctttt ttgcgatctt acgcaaaatt gtagacacga gactttggag
      181 gcactcggga tttctccgca tcttgccacc actttcgagc ataacggagt cttgaagtcc
      241 ggattgacga aaccggaatg taattatctt tgcggcgatc tttatgtctg caaagattcg
      301 gttccggttt cggtcaaggg aaaatatacg aaccacgacg actgcctcgt cggcgaagga
      361 gtgtattgtt tcaaaaaaga agatcttatc tatgaacaat gtctcgccga aggaatctcg
      421 ccgggccaat ccgcctatta ttactgtgcc gctccttcgt atgactgcag caaaacttcc
      481 cagacttttt ccgattcgga atcctgcgaa aacaccatgc atggaaaatg ctatcgcgtg
      541 agcaacgact gtgaagccga gtgccctccg gaaggccttg gtcaatatta ttggtgcaat
      601 cccgcaggcg gatgctcgag cgggaattat ctatcgggag aagaatgcga gcaaggcgac
      661 gaagaaaagg tttgctattc ggatttcgga tcttgttctt ccgcctgcag aacttctggg
      721 gtaatttcct gcaatcttcc ggaaacaggc tccggagacg atattgcggt ctctctcgaa
      781 gccgatccgc aatgcgttcc cataagatct ctaaattacc agtcgacgct ctcttgggaa
      841 ataggtatcg gtaacagctg cggcgaaggc gaggagcctt ccaacaacag gttctcctgt
      901 gtcagttgca atatgccatc tccgagcgtc gaaagcggca gcgtcgcggt ttctgcggat
      961 agaaccaccg aatacggcat ttcctgcacc cgggccgcct ataggtgctg ttgggaagaa
     1021 gacgaggact acagttgcaa ttgcgtcgga gaagaacaag aaagagtttg cgaaacctgc
     1081 acccgcgcca tcactatttg cgacgacgaa cacgctgctt ccacgggaat cgccaaaaca
     1141 tcgatacgag tggtccctaa acccgttatc caaagcttca ccgccgatcc agtggatatt
     1201 ctctataccg ccgaaaacag agtggctgaa tccggataca ataagttctc gactcttagt
     1261 tggacttctt tagagagtca attcgatttt ccgataacga cttcctgcgt cgtcattagg
     1321 gacgacggaa cttcggaggc ggctggtttg aaccaaagcg gttcccgcac ggtgtctcct
     1381 tcgagaacca ccatttatac gcttcaatgc cggaacagcg acgacgaaga gccggaaact
     1441 tgctattcgg attcggaaac ggaaaacgcg accgtcaggg tattcggagc gggcatagaa
     1501 gaaccggggc cgcagaccta tctcgaagga ttcggtaaaa agatcggtca aatcattaac
     1561 gggtttttgg atcttccgga ataggggaaa cattcgaatc ccgcgaaagg cgggattttt
     1621 gttatactta acattatgaa aaaactgatt gtcgccaatt ggaagatgaa tccggagacg
     1681 attgcggagg ccaggtcttt gctcagccga tctcttacgg taatatccaa aaataaatcg
     1741 atcgattttc tggcagccgt gccaacgccg tatctcggcg atgcggcctt caaaaaacgc
     1801 gccaagtatc tggccggcca gaacgtctct tgggccgaaa acggggcttt gaccggagag
     1861 ttctccgcga aaatgctcta ttccttggga gtgaaatacg cgatagtggg tcattccgag
     1921 cgccgggaat atttttttga gaacgaagag atcatcgcca agaaaattca gaaatgtttc
     1981 gaagctggaa taagacctat tctttgcgtc ggcgaatcga cggccatcag acggaaagga
     2041 ttggatctcg tcaaaatcta tctcaaggat cagctgcggc agatcatcgc cggcacagaa
     2101 cacagcctta tatcgaaaat cgtttttgtt tacgaaccgg tttgggccgt cggagcggaa
     2161 gaggccgacg atccggagag cagtgccgaa ctcataaacc acatgaaat
//
LOCUS       DAOPQT010000097         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_53768_length_2201_cov_3.049860, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000097 DAOPQT010000000
VERSION     DAOPQT010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53768_length_2201_cov_3.049860"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="PKG74_02675"
     CDS             complement(<1..1282)
                     /locus_tag="PKG74_02675"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOB90206.1"
                     /translation="MDFSGFLVWPGRDPGIKKDALALAEDFINRPIGFSFSEIEKRAL
                     RPFFTNPDHSVYLIHTLPEAITARLEARYSRMNNRRGLRGVFVDSCLPELLASRIPEA
                     ENDPKAWLDSRGIKNLDQFSEDPLGKIAFEEFSRHFCCDPGYLERFASSELTNRFFGN
                     WIERFGHGSIGRMAKIAIGCEGVSLMAAKALEEPRPGAGFMELSTRYVNLSGAEVYPI
                     ADIVRLFSPALAERVSMMIASDFELYRNLSDPNGAFAGFLEKRYGHHFKDQSQLKTAI
                     GGEVCDVLGNLLPGATLTSLGVSVSGEAFGSLIKHLLLHGTPETIALASVILAEAEKT
                     GSNQLARHFLPSKKEREFWGFLEPESFRGLKAKEVVFASQYPSIVTAFDLLRLKPRFS
                     KRSDGEIAAMIRFDREDNDKLPPEFEGVGMAFKFI"
     gene            1372..>2201
                     /locus_tag="PKG74_02680"
     CDS             1372..>2201
                     /locus_tag="PKG74_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOB90207.1"
                     /translation="MLKGKKNLDLPEIEKKVLDFWEDGRIFEKSLERRKNKKAFKFYE
                     GPPYANGKPGIHHVLARVFKDIILRYKAMEGFYVPRRAGWDTHGLPAEIATEKELGIK
                     NKKEIEAYGIDRFNAEAKKGVMKYQREFELMTERIGYWLDLKNAYITYTNPYIEGLWK
                     VFKKISNRGFLKRDYKILSWCPRCETPLASHELAQPGAYKLVKDPSLYVKFKLKNEDN
                     TYLLVWTTTPWTLPANMLVAVNPNLVYSKYEINNESVWSGIVPPGAENARVVETASGK
                     "
BASE COUNT          563 a          574 c          572 g          492 t
ORIGIN      
        1 taatgaactt gaaagccatg ccgacgccct cgaattccgg aggcagcttg tcgttatcct
       61 cgcgatcgaa tctgatcatg gcggcgatct ctccatccga tcttttgctg aagcgcggct
      121 tcagtcgtaa aagatcgaaa gccgtaacga tcgagggata ttggcttgcg aaaaccactt
      181 ccttggcttt taggccgcgg aagctttccg gctccaggaa tccccaaaac tctcgttctt
      241 ttttgctcgg cagaaaatgc ctggcgagct gattgcttcc ggttttctcg gcttcggcga
      301 gaataacgga agcaagagcg atcgtctccg gagtcccgtg gagcaacaag tgcttgatca
      361 ggcttccgaa agcctctccg cttacggaaa cgcccaaaga agtgagcgtc gctccgggca
      421 aaagattgcc gagaacgtcg cagacctctc cgccaatagc ggttttgagc tggctttggt
      481 ctttgaagtg gtggccgtac cgtttctcga gaaatcccgc aaacgctccg ttcggatcgc
      541 tgagatttcg gtaaagctcg aaatccgaag cgatcatcat tgagacgcgc tccgcgaggg
      601 ccggggagaa aagccttacg atgtcggcaa tcggatagac ttccgctccg gacaaattga
      661 cgtaccttgt cgagagctcc atgaatccgg cgccgggtcg aggctcttcg agagctttag
      721 cggccatgag agatactcct tcgcaaccga tggcgatttt agccattcgg ccgatcgatc
      781 cgtgtccgaa ccgttctatc cagtttccga agaaacggtt ggtaagttcg gacgatgcaa
      841 acctttcgag gtatcccgga tcgcagcaga agtggcggct gaattcctcg aaagcgatct
      901 ttcccaaagg atcttcagag aactggtcga ggtttttgat gcctcgactg tcgagccagg
      961 ctttgggatc gttctccgct tccgggatgc gcgaagcgag gagttcgggc aagcaggaat
     1021 cgacaaagac tccgcgaagc ccgcgccgat tgttcatccg cgagtatctc gcttccagtc
     1081 ttgcggttat ggcctccggc aaggtgtgaa tcagatagac cgagtgatcg gggttggtga
     1141 aaaacggcct gagcgccctt ttctcgattt ccgagaaaga gaatcctatc ggccgattga
     1201 tgaagtcttc ggccaaagcc aaggcgtctt tctttatccc cggatccctt ccgggccaaa
     1261 caaggaaacc gctgaaatcc atttcgaaaa cccccttcga tttgtcaaag atgctagccg
     1321 caatgtatcc tacttttatt cgaaaggcaa actcattaca ataaaaacac tatgctgaaa
     1381 ggaaaaaaga acctggatct tccggaaatc gagaaaaaag tgcttgattt ctgggaagac
     1441 ggccggattt tcgagaaaag cctggaacgg cgaaaaaaca agaaagcctt caagttttac
     1501 gaaggtccgc catacgcgaa cggcaagccg ggaattcatc atgtgctcgc cagggttttc
     1561 aaggacatta tccttcgcta taaggcgatg gagggctttt acgttccgcg gcgcgccggt
     1621 tgggataccc acggcttgcc cgcggaaata gccaccgaaa aagaactcgg catcaagaac
     1681 aagaaggaaa tcgaagccta cggcatcgac agattcaacg ccgaagccaa aaaaggcgtc
     1741 atgaaatacc agcgtgaatt cgaattgatg accgagcgca tcggctattg gctcgatctc
     1801 aaaaacgctt acatcaccta taccaatcct tatattgaag ggctttggaa ggttttcaag
     1861 aaaatcagca atcgcgggtt cctgaaaagg gattacaaga ttttgtcctg gtgcccgcgc
     1921 tgcgaaaccc cgctcgcttc gcacgagctg gcccagcccg gcgcttacaa actggtcaaa
     1981 gatccgtcgc tctacgtcaa gttcaaactg aaaaacgaag acaacactta tcttcttgtt
     2041 tggacaacca cgccctggac tttgccggcc aatatgctcg tggcggtaaa tcccaacctc
     2101 gtttattcca aatacgagat aaataacgaa tctgtctggt ccggcattgt tccgcccgga
     2161 gcggaaaacg caagagtcgt ggaaacggct tcggggaaag a
//
LOCUS       DAOPQT010000098         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_55299_length_2155_cov_2.785714, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000098 DAOPQT010000000
VERSION     DAOPQT010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55299_length_2155_cov_2.785714"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="PKG74_02685"
     CDS             <1..1095
                     /locus_tag="PKG74_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOB90208.1"
                     /translation="FRVYSGKLSRGSYILNSTKNNQERIGRILRMHANDREEVDEIYA
                     GEIGALVGLKNTTTGDSLCDPDKPIILEKITFPQPVIEQRVSPKTKADQEKMGMALHR
                     LAEEDPTFRVSGDPETGETIIAGMGELHLEVLVERMRREFSVEADVGRPQVAYKETIL
                     GSAEAEGKYVRQSGGRGQYGHVWLKVSSKDRGDGFTFVDAIKGGAIPREFIPAVEKGV
                     REALDRGVIAGYTLTDLEVTLYDGSFHEVDSSEAAFKIAASMALQEGAKKAKPVILEP
                     VMKIQVIIPSEFLGDITGDLSSKRGKIEAMNDRANTKVVDAMVPLSEMFGYATKLRSM
                     TQGRGAFTMEFDHYDIVPKNLEEAIISGKK"
     gene            1194..>2155
                     /gene="tuf"
                     /locus_tag="PKG74_02690"
     CDS             1194..>2155
                     /gene="tuf"
                     /locus_tag="PKG74_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOB90209.1"
                     /translation="MAEKEKFSRTKPHVNVGTIGHVDHGKTTLTAAIIHVLYLKGLAK
                     KEEKVDDIDKAPEEKARGITIALHHSEYESEKRHYAHIDAPGHADYIKNMITGAAQMD
                     GAILVVSAADGPMPQTREHILLAHQVGVPAMVVFLNKVDMVDDPELVALVEAEVRELL
                     SKYSYKGDEVPIIKGSALKALEASSLDDPAVKPILELVDALDSYIPEPKRETDKPFLM
                     PIEDIFTIEGRGTVVTGRIERGVVHVNDEVELIGLKPTAKTVVTGIEMFNKTLDEGQA
                     GDNVGVLIRGIKKEDVERGQIIAKPGSVTPHTDFEAEVYLLTKE"
BASE COUNT          592 a          557 c          592 g          414 t
ORIGIN      
        1 ttccgcgttt acagcggcaa gcttagccgg ggaagctata ttttgaattc gaccaaaaac
       61 aaccaggaaa gaatcggccg cattctgcgg atgcacgcga acgacaggga agaggtggac
      121 gaaatttacg ccggagagat cggcgccctt gtcggactca aaaacacgac gaccggcgat
      181 tcgctttgcg atccggataa gccgatcatt ctggaaaaaa tcacttttcc gcagccggtc
      241 atagagcaga gagtttcgcc gaagaccaaa gccgaccagg agaaaatggg catggccctt
      301 catcgtctgg ccgaggaaga tccgactttc agggtctccg gagatcccga aaccggcgaa
      361 acgatcatcg ccggaatggg tgagcttcat ctcgaagttc tcgtcgaaag aatgcggcgc
      421 gaattttccg tcgaagccga cgtcggccgg ccgcaagtcg cctacaaaga gactattttg
      481 ggttcggccg aagccgaagg caaatatgtc aggcagtccg gaggccgcgg ccagtacgga
      541 cacgtctggc ttaaagtcag ctccaaggat cgcggagacg gattcacttt cgtcgacgcc
      601 atcaaaggag gagccattcc ccgggaattc attccggccg tcgaaaaagg cgtgcgcgaa
      661 gctttggatc ggggtgttat cgcgggctac acgttgactg acctcgaggt gactctttac
      721 gacggctctt tccacgaagt cgattcgtcc gaagccgctt tcaagatcgc cgcttccatg
      781 gctttgcagg aaggcgccaa aaaagccaaa ccggtgattt tggagccggt gatgaaaatc
      841 caggtgatca tcccgtcgga atttttggga gacatcaccg gagacctttc ttcgaaaagg
      901 ggaaagatcg aagcgatgaa cgaccgggcc aataccaaag tggtcgatgc catggtcccg
      961 ctttcggaga tgttcggata cgccaccaaa cttcgttcga tgacccaggg aagaggggcg
     1021 tttaccatgg agttcgacca ctacgatatc gttccgaaga acctggaaga ggcgatcatt
     1081 tccgggaaga aatagccgga actccaaaaa cccagaaaaa accttgacaa atacccccat
     1141 caatcagtaa tattttaagg tcgtaaagtc ataaaaaata atcaaattca aaaatggccg
     1201 aaaaagagaa attttcaaga actaaaccgc acgtcaacgt cggcaccatc ggtcacgtcg
     1261 accacggcaa gaccactttg acggcggcga ttatccacgt tctttacctc aaaggtttag
     1321 cgaagaaaga agagaaagtc gacgatatcg acaaggcgcc ggaagaaaag gcgcgcggta
     1381 tcaccatcgc cttgcatcac tcggagtacg agtcggaaaa aaggcattac gcccacatcg
     1441 acgctccggg acacgcggat tacatcaaaa acatgattac cggcgccgcc caaatggacg
     1501 gagcgattct cgtcgtttcc gccgccgacg gcccgatgcc tcagacaagg gagcatatcc
     1561 ttttggccca tcaggtcggc gttccggcca tggtggtatt cctgaataaa gtcgatatgg
     1621 tggacgaccc cgaattggtg gcgctcgtcg aagccgaagt gagggagctt ctttccaaat
     1681 acagctacaa aggagacgaa gtgccgatca tcaaaggttc ggccttgaag gcgctcgagg
     1741 cgagttcgct cgacgatccg gcggtcaaac cgatcttgga attggtggac gcccttgatt
     1801 cttatattcc ggaaccgaaa agagaaacgg ataagccgtt cctgatgccg atcgaggata
     1861 tcttcacgat cgaaggccgc ggtaccgttg tcaccggccg catcgaaaga ggcgtggtcc
     1921 acgtgaacga cgaagtggag cttatcggct tgaaaccgac cgctaaaacc gtggtcaccg
     1981 gaatcgagat gttcaacaag accttggacg aaggacaagc cggagacaac gtcggtgttt
     2041 tgatccgtgg catcaagaaa gaagacgtgg aacgcggaca gatcatcgcc aaacccggat
     2101 cggtcacgcc gcataccgac ttcgaggccg aagtttatct tttgaccaaa gaaga
//
LOCUS       DAOPQT010000099         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_56187_length_2130_cov_3.926747, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000099 DAOPQT010000000
VERSION     DAOPQT010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56187_length_2130_cov_3.926747"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PKG74_02695"
     CDS             complement(<1..849)
                     /locus_tag="PKG74_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOB90210.1"
                     /translation="MKEKIKIVWGHIKNFAKTRKKATIALIVLLVISGVFAAKAIKAR
                     NVETKYVLAAARTDTISSVVAGGGVVAAESQVDIKPKVSGDVVYVGVKVGDKVKKGDL
                     IARIDETDARKSVRDAEINLENAKLSYEKSIKPATELEIRQAENALFKAEDNLDKEAL
                     AAYDAALSGLAKLPEAISGIYDLIYDTDFGFEMTNLNYYKTLALLNKDGAAKAASLTE
                     ENYKKAKSLYKSVSSDLIAFDSEGDEILNYLESVYEGVKAGLEAAQAERNFIMLVRDD
                     FDSRNQD"
     gene            complement(857..1339)
                     /locus_tag="PKG74_02700"
     CDS             complement(857..1339)
                     /locus_tag="PKG74_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90211.1"
                     /translation="MNIKDFFESKTFKGTLVGLTVFVVLLWIFQLGVGIGYRKAYFSG
                     RAGDNFRLIFGGRPQNHGVPPFRVFYPAAEQGVFSGYGTTGTIISINLPRFALETPEN
                     IERSVLIGEKTEIRKYRDNLRPEDLQLEDNVVIIGSPSQNGEIEARLIRYLPSEQFQK
                     "
     gene            complement(1343..1729)
                     /locus_tag="PKG74_02705"
     CDS             complement(1343..1729)
                     /locus_tag="PKG74_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90212.1"
                     /translation="MELFSGIKPPSGLEDKIIKKIAAEERKIAVVKTAFFGVSGLTAA
                     TASIPAFLKAVDLMEQSNFLAYVSLVFSDSRIVLSNLSEFLMATIEAVPATEISIALN
                     LILAGLWSIAAVAKSIPAIRTVKLRI"
     gene            complement(1753..>2130)
                     /locus_tag="PKG74_02710"
     CDS             complement(1753..>2130)
                     /locus_tag="PKG74_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOB90213.1"
                     /translation="GNIDPKMNIRAWLFTIARNLVIDSARKKKTVSFSDFTDQNNRLL
                     EDMIQSDDLSPEELLIRDSDKKALRKLVSRLPLIYRETLILKYDEGMDFKEIAGILGI
                     SPNTVKSRHQRAILRLKTALASE"
BASE COUNT          490 a          513 c          516 g          611 t
ORIGIN      
        1 atcctggttc cggctgtcga aatcgtcgcg gacgagcatg atgaaattgc gttccgcttg
       61 ggccgcttcc agtccggctt tgacgccttc gtaaaccgat tccaggtaat tcaagatttc
      121 atctccttcg gagtcgaaag cgatcaaatc cgaggaaacg gatttataga gacttttggc
      181 ttttttgtaa ttttcttcgg tgagcgaagc cgccttggcc gctccgtctt tgttcaaaag
      241 agccaaggtc ttgtaataat tcaaattggt catttcgaac ccaaaatcgg tatcgtagat
      301 caagtcatag attccggaaa tcgcttccgg cagtttggcg agcccgctca aagcggcgtc
      361 ataggcggcc aaagcctctt tatccaaatt atcttcggct ttgaacaagg cgttttccgc
      421 ctgtcttatt tccagctcgg tcgccggctt gattgatttt tcatagctca atttcgcgtt
      481 ttccaaattg atttccgcgt ccctgacgga ttttctcgca tccgtctcgt cgatacgcgc
      541 gatcagatct ccttttttga ctttgtcgcc gactttgacg cccacgtaaa ccacgtctcc
      601 ggaaaccttg ggtttgatat cgacttggct ttcggcggcg acaacgccgc ctccggcgac
      661 cacggaagaa atggtatcgg ttctcgcggc cgccaaaacg tattttgttt ccacgtttct
      721 cgccttgatg gctttggcgg cgaagacacc ggaaataacg agaagaacga tcaaggcgat
      781 cgtcgctttt ttacgggttt tggcgaagtt ttttatatgt ccccagacga tttttatttt
      841 ttctttcatg ttgtttttat ttttgaaatt gctccgacgg caaatacctg attaatctcg
      901 cttctatttc tccgttttgg ctcggagagc cgatgatgac gacgttgtct tcgagttgca
      961 gatcttccgg acgcagattg tcccggtatt tcctgatttc tgttttttcg cctatgagta
     1021 ccgaccgctc gatattctcc ggggtttcga gggcgaatct cggaagattg atgctgatga
     1081 tcgttcccgt tgtcccgtat ccggagaaaa ctccttgttc cgcggccgga taaaacaccc
     1141 ggaaaggagg aacgccgtgg ttttgcggtc ggccgccaaa aataagacga aaattatctc
     1201 cggcacggcc ggaaaaataa gccttgcggt agccgattcc gacgcccaat tggaatatcc
     1261 agaggagaac gacaaagacc gtcaggccga ccagggtgcc tttaaaggtt ttcgactcga
     1321 aaaaatcctt gatgttcatt ttctatattc tgagctttac tgttctgatc gcgggaatgc
     1381 ttttggcgac cgcggcgatt gaccagagtc cggcaagaat caggtttagg gcaatgctga
     1441 tctcggtcgc cggcacagcc tctattgtcg ccatcaagaa ttccgacaga ttcgatagga
     1501 cgatccggct gtcggaaaaa acaagtgaaa cgtaagccag aaaattgctt tgttccatga
     1561 gatcaacggc tttaaggaaa gcggggatag aggctgttgc ggccgtaagg ccggaaacgc
     1621 cgaaaaacgc agtttttacg acagcaattt ttctttcttc cgcggctatt ttcttgatga
     1681 ttttgtcttc aagcccggaa ggcggtttta ttccggaaaa aagctccata cctgaatata
     1741 cggataaacc cgctattcgg atgcaagggc ggttttaagc ctcaaaatag ccctctggtg
     1801 gcggcttttc acggtattcg gggaaatacc caagattccg gcgatctcct taaaatccat
     1861 tccttcgtcg tatttcagga taagagtttc ccggtaaatc aaaggaagtc tggatacgag
     1921 ctttcttagg gcttttttgt cggaatcccg gatcaaaagc tcttccgggg aaagatcgtc
     1981 gctttggatc atatcttcca gtaaacggtt gttttgatcg gtgaaatcgg aaaaagaaac
     2041 ggttttcttt tttctcgccg aatcgatcac gagattcctc gcgatcgtga aaagccaggc
     2101 ccggatattc attttcggat cgatatttcc
//
LOCUS       DAOPQT010000100         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_56771_length_2114_cov_3.446819, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000100 DAOPQT010000000
VERSION     DAOPQT010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56771_length_2114_cov_3.446819"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKG74_02715"
     CDS             complement(<1..201)
                     /locus_tag="PKG74_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90214.1"
                     /translation="MNINDIQKWEREFSAKKGIAQDEEKATKIAVLKLVEEVGEACKA
                     ILEKKWEEIPAESETSLDPSQPA"
     gene            complement(480..905)
                     /gene="rplK"
                     /locus_tag="PKG74_02720"
     CDS             complement(480..905)
                     /gene="rplK"
                     /locus_tag="PKG74_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOB90215.1"
                     /translation="MAKPIKTTLKLQIKAGAANPASPVGPALGQHGVNIQEFVKQFND
                     ATKDMGSDIIPAEITIYEDRSFTFKLKTPPASDLLRKAAGIEKGSAVPNKNKVGKVSK
                     EDLRKIAEKKAVDLNANDVEAAEKIIAGTARSMGIEVVD"
     gene            complement(927..1481)
                     /gene="nusG"
                     /locus_tag="PKG74_02725"
     CDS             complement(927..1481)
                     /gene="nusG"
                     /locus_tag="PKG74_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOB90216.1"
                     /translation="MEKTTPEQTETRHWYAVHTYSGYEDAVSRYLKQRIESLGVEDKI
                     FNVIVPKEKKVKIKNGRRTVVDEKIYPGYVLVDMVMGPDTWYIVRNTPRVTGFVGADT
                     TTPTPLSKAEVDSLMAKMTGEEPSFRIDYKAGDLVRITDGPFKDADAKISEVDEARGR
                     VKVLVSIFGRDTAVEIDALQVKKI"
     gene            complement(1488..1673)
                     /gene="secE"
                     /locus_tag="PKG74_02730"
     CDS             complement(1488..1673)
                     /gene="secE"
                     /locus_tag="PKG74_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOB90217.1"
                     /translation="MALKKFIKDSYEELRKVNWPSRKDTFRMVLLVIVFAVIVSLLLF
                     SADSFFTFLMKKFILKI"
     gene            complement(1704..>2114)
                     /locus_tag="PKG74_02735"
     CDS             complement(1704..>2114)
                     /locus_tag="PKG74_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086081.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOB90218.1"
                     /translation="DADVDGAHIRTLLLTLFYRYFEKLIENGHIFIAQPPLYKVQKGK
                     EFHYGYTDAEKDALLKKMGEQSVVSRYKGLGEMNPEQLWETTMNPETRMMKVVTVDDA
                     KEADRLFDIFMGSEVEPRKRYIQIHGASVKNLDI"
BASE COUNT          469 a          535 c          500 g          610 t
ORIGIN      
        1 tgccggctgc gaagggtcga ggctggtttc gctctcggcc gggatctctt cccacttttt
       61 ctccaagata gccttgcacg cttcgccgac ctcttcaacc aatttcagaa cggcgatctt
      121 ggtggctttt tcctcgtctt gggctatgcc ctttttggca gaaaactccc tttcccactt
      181 ttggatgtcg ttgatattca ccggacttat tataaacgaa ctggtaattt tgggaataca
      241 actacgccaa atcctgcctc tttagcctct aggccgcaaa cgtaaagcca aataaaaggt
      301 ttttcacggc gaaaatagct attagtcgac gatttctatc cccaagagaa taagaaggtc
      361 taaaatagcg gcaatcagta tcccggcggc aatgcctttc acgattttct tcattttttg
      421 tttaacaaaa agcgaccttt tgggtcgctt ttagcggtta cagtattatg ttcgcgaggt
      481 taatccacaa cctctatccc catcgagcgc gcggtgccgg caatgatctt ctcggcggcc
      541 tccacgtcgt tcgcgttcaa atcaaccgct tttttctcgg cgattttccg caaatcctcc
      601 ttactcactt ttcccacttt gttcttgttc ggaacagccg atcctttttc aataccggcg
      661 gctttgcgga gcaaatccga agccggcgga gttttcagct tgaaggtgaa gctccgatcc
      721 tcgtaaatgg tgatttcggc cggaatgatg tcgctgccca tatccttggt ggcgtcgttg
      781 aactgtttga cgaattcctg gatattcacg ccgtgctggc ccaaagccgg gccgaccgga
      841 gaagccgggt tggcggcgcc ggcttttatt tggagtttca aagttgtttt gattggtttg
      901 gccatattag caggaaatat cttaaactaa atctttttga cttgcaaagc gtcgatttcc
      961 acagccgtgt cgcgtccgaa aatggaaacc aaaactttga cccggcccct ggcttcgtcc
     1021 acttccgata ttttggcgtc ggcgtccttg aacggaccgt cggtgatgcg gaccaagtct
     1081 cccgctttgt aatcgatccg gaagctcggt tcctcaccgg tcattttcgc catcagggaa
     1141 tcgacttcgg ctttggagag cggggtcggc gtggtggtgt cggcaccgac gaacccggtg
     1201 acgcgcgggg tgttgcggac gatataccaa gtatccggcc ccatgaccat gtcgacgagc
     1261 acgtatcccg ggtatatctt ttcgtcgacg acggtccggc gcccgttttt gatcttgact
     1321 ttcttttcct tgggaacgat aacgttgaaa attttgtctt cgacgcccaa cgattcgata
     1381 cgctgcttca aataacggct gacggcgtct tcgtatcccg aataagtgtg caccgcgtac
     1441 cagtgccgag tttcagtctg ttccggagtg gttttttcca taaaatttta aatcttcaaa
     1501 atgaatttct tcatcagaaa agtgaaaaac gaatctgcgg aaaaaagaag caaagacacg
     1561 atcacggcga aaacaataac gagaagcacc atgcggaagg tgtcttttct gctcggccag
     1621 ttgaccttgc ggagttcttc gtaggaatct ttgatgaatt ttttgagtgc catgtaagct
     1681 ttgtaaggtt ataattttta aagctagatg tcaagatttt tgaccgaagc accgtggatc
     1741 tggatatatc tttttctcgg ctcaacctcg cttcccatga aaatgtcgaa aagccgatcc
     1801 gcttccttgg catcgtctac ggtcaccact ttcatcatcc tcgtttccgg attcatggtc
     1861 gtttcccaaa gctgttccgg attcatctct cccaaacctt tatagcggct gaccaccgac
     1921 tgttcgccca ttttcttgag taaggcgtct ttttcggcgt cggtataacc ataatggaat
     1981 tctttgcctt tttggacttt ataaagcggc ggctgggcga tgaaaatatg cccgttctcg
     2041 atcagttttt cgaaataacg gtagaaaagc gtcagaagca aagtccggat atgggcgccg
     2101 tcgacgtcgg catc
//
LOCUS       DAOPQT010000101         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_58025_length_2081_cov_3.615992, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000101 DAOPQT010000000
VERSION     DAOPQT010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58025_length_2081_cov_3.615992"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>227)
                     /locus_tag="PKG74_02740"
     CDS             complement(<1..>227)
                     /locus_tag="PKG74_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90219.1"
                     /translation="VYTDIKQNRIPNKLIVAGLICAVVLYASVLIYDRFFIGLQTNLD
                     YMIEALLNTAAAMIAGYVIWDLKLWSPGDAK"
     assembly_gap    228..237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(283..1221)
                     /locus_tag="PKG74_02745"
     CDS             complement(283..1221)
                     /locus_tag="PKG74_02745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOB90220.1"
                     /translation="MEFCVTNLCNNRCVMCTNSAEFSDPKNSQGYEFDQLIERIGQRA
                     EKLLKTREPINITGGEPTIVPHFVKLLNWLRKNFPRNEIVCVSNGRMFSYEDFAKQVL
                     RVDNLKLEIAFHGHTPRLYDEISGVKGGFPQTVQGVKNILRFRNDSHLIEIRTVIIRQ
                     NHKKLDEILGFVHKNFKGLDTVVLIFHELEGQCGDHLDKVVVSYKDIEKEVIRNIEKW
                     SEKIDDLRLYHFPLCVLPPKLWPHTWRTLPPREVSFPDECAKCLYRKYCMGVHNDYRH
                     IVGDEEFNSIAAKVPLRTSRGQEFVYHPIVGIDDKS"
     gene            complement(1235..1435)
                     /locus_tag="PKG74_02750"
     CDS             complement(1235..1435)
                     /locus_tag="PKG74_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90221.1"
                     /translation="MAKKEEKTYIVTYRSEEHFEVVGWVWASNLLEAKRKAKAELAEQ
                     ARRYDVTLAEIAEYKDASEISF"
     gene            complement(1464..>2081)
                     /locus_tag="PKG74_02755"
     CDS             complement(1464..>2081)
                     /locus_tag="PKG74_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90222.1"
                     /translation="IHDYLTQVPGSFDQLKKGLASIRSIIKRKNLKMHLGSNTTVVKQ
                     NYPYLPQIGKFILDQGIKDSEFIFVDCNEGGARRYFEELVPKISEAAPFIRQCLDIGQ
                     KAGALHWDIRYVPLCYFPDHLHRISELHEIKIFNTEHIAQDFVDFDALESRKRWARKK
                     TLRCQGCALYNSCEGIWMEYLKRYGDEEFSPVKTISKRQKKMLDS"
BASE COUNT          503 a          495 c          493 g          580 t
ORIGIN      
        1 aatttggcgt cgcccggaga ccataacttc aaatcccaga tgacgtaacc ggcgatcatc
       61 gccgccgcgg tattcaacaa agcttcgatc atataatcga gatttgtttg aagaccgatg
      121 aagaagcgat cgtagatcaa aacggaagca taaaggacga cggcgcaaat cagcccggca
      181 acgatgagct tgttcgggat tctgttctgc ttgatatccg tataaacnnn nnnnnnncaa
      241 tcgctccgat caaaaacatc ggcgcgagaa aaaggaaatc gatcatgatt tgtcgtcgat
      301 cccgacgatc ggatgataaa cgaactcctg tccccggctt gttctcaagg gaactttcgc
      361 cgcgatggaa ttaaactctt cgtctccgac gatatgacga taatcgttat gaacgcccat
      421 gcaatatttc cggtaaaggc atttggcgca ttcatccgga aaagaaacct ctctcggagg
      481 caaagttcgc caagtgtgcg gccaaagctt tggcggcaaa acgcacaaag gaaaatgata
      541 aagcctcaga tcgtcgattt tttcggacca tttctcgatg ttccggatca cttctttctc
      601 gatgtctttg taagaaacca ccactttgtc caaatggtct ccgcattggc cttccaattc
      661 gtgaaaaatc agcacgaccg tgtcgagccc tttgaaattc ttgtggacaa aacctaggat
      721 ctcgtcgagc ttcttgtggt tttgcctgat aatgaccgtt cttatctcta taagatggct
      781 gtcgttccgg aaacgcaaaa tgttttttac tccttggacc gtctggggaa aacctccttt
      841 aaccccggag atttcgtcgt agagccgcgg agtgtgtcca tggaaagcga tttccaattt
      901 gagattgtcg acgcgcaaaa cctgtttggc gaaatcctcg taggagaaca tccggccgtt
      961 cgatacacag acgatttcgt tgcgggggaa gtttttccgg agccagttca aaagtttgac
     1021 gaaatgcgga acaatagtcg gttctccgcc ggtgatattg atcggctctc tggtcttcag
     1081 aagcttttcg gcacgctggc cgatcctttc gatcaactgg tcgaattcgt atccttggct
     1141 gtttttggga tcgctgaact cggccgaatt ggtgcacatg acgcatcggt tgttgcacag
     1201 attggtgacg caaaattcca tcagacgata tggatcaaaa gcttatttcc gatgcgtctt
     1261 tgtattcggc tatttccgcc agggtgacat cgtagcgacg agcctgctcc gccaattcgg
     1321 ctttggcctt gcgctttgcc tcgagcaagt tactcgccca tacccatccg accacttcga
     1381 aatgttcttc gctccggtaa gtaacgatat aagttttttc ttctttttta gccattgtgt
     1441 tttatcgatt tgtttttttt cgatcaggaa tcgagcattt tcttttgtcg tttgctgatt
     1501 gttttaacgg gagaaaactc ctcgtcgccg taacgcttca ggtattccat ccaaatgcct
     1561 tcgcaggaat tatacaatgc gcagccttgg cagcgcaaag ttttcttacg agcccatctt
     1621 ttacgcgatt ccaaggcgtc gaaatcgacg aaatcctggg caatatgctc cgtgttgaat
     1681 atttttattt catggagttc gctgatccgg tgcaaatgat cgggaaaata acaaagcggc
     1741 acgtatctta tgtcccaatg gagggcgccg gctttttggc cgatatcgag acattgccgg
     1801 ataaacggcg ccgcttcgga gattttcggg accaactcct cgaaataacg cctggctccg
     1861 ccttcattgc aatcgacgaa gatgaattcc gaatctttga tcccctggtc caggatgaat
     1921 tttccgattt ggggaagata agggtaattc tgcttgacga cggtggtgtt ggagccgaga
     1981 tgcattttca aattcttccg cttgatgatc gaacggatac tggccaatcc ttttttaagc
     2041 tggtcgaaac ttccggggac ctgggttaaa taatcgtgga t
//
LOCUS       DAOPQT010000102         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_58787_length_2061_cov_2.352443, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000102 DAOPQT010000000
VERSION     DAOPQT010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58787_length_2061_cov_2.352443"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="PKG74_02760"
     CDS             <1..1271
                     /locus_tag="PKG74_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90223.1"
                     /translation="SGTPGYTGAYMSGYYGLDLFTGGQSRVHVLQNGNIGIGTISPVQ
                     LLEVSGTNGKGISLTNTSGYGNTSNIDFYGFNNTSLIRRFASIQGLVTGEDTQNGDIG
                     AILFSTKPLLGDTSLTERMRITSGGNVNIGTTAPFIGQLNVNKANVGALGGDLWITNA
                     AANTTGNSARLSFGLDNTTDTTSNAYIHALLTNSSGYLTDLAFGTYGGGSLSERMRIT
                     SEGNVGIGTVSPGAKLNVAGGDIKMGDASNPRGLNILQHYLSLGETESGASTILGNNI
                     KVTDGVDETVQVSASAADGPTYLRLNYSNGITFHRIAPAAVGTQYASSTGELMRITTA
                     GNLGIGTTVPAAKLAINGGLHVGGDSDPGDNNALIDGTLTVTGNSNTCNMVSYTGGGG
                     TTQCPAGFYTWSGVALTSGYMLCCKVSNPI"
     gene            complement(1315..>2061)
                     /gene="recA"
                     /locus_tag="PKG74_02765"
     CDS             complement(1315..>2061)
                     /gene="recA"
                     /locus_tag="PKG74_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003349162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOB90224.1"
                     /translation="KAAFIDAEHALDPDRAKKIGVKVSELLLSQPDSGEEALNIMESL
                     VRSGMIDVIVVDSVAALTPKAEIEGEMGDQFIGLQARMISQAMRKITAIANKSKTLII
                     FINQLREKIGMMGYGEPTTTPGGRALKFYSTIRIDLRRIAQIKKGEEVVGNRVRAKVA
                     KNKVAAPFKTAEFDIMFADGISYEGDVMNVAIGQGIIKKSGNTYSFGEEKLGVGTEAA
                     KARLKEDKKLFREIEKKTREALDEAEKKNK"
BASE COUNT          478 a          531 c          502 g          550 t
ORIGIN      
        1 attcgggaac cccgggttac accggagctt acatgtccgg ctattacgga ttggatcttt
       61 ttaccggagg acaaagtaga gtacatgtct tacaaaacgg taatattgga atagggacta
      121 tcagcccggt tcagttattg gaggtttccg gtacaaacgg taagggcata tctctaacga
      181 atacatccgg ttacggcaat acttccaata tcgatttcta cggtttcaac aatacgtctc
      241 ttataagaag attcgcctcg attcaaggcc tggtcacggg agaagatact caaaatggcg
      301 atataggggc gattcttttt tcgaccaagc cactccttgg tgatactagc ttgacggaaa
      361 gaatgaggat tacctctgga ggtaacgtca atatcggaac gacggcgcct tttatagggc
      421 aattgaatgt aaataaagct aacgtcggag ctctcggcgg agatctatgg ataaccaatg
      481 ccgcagccaa caccacgggt aattcggctc gtctttcctt cggcctcgat aacactaccg
      541 atactacttc aaacgcttat atccacgcat tattaaccaa tagttccggc tatttgacag
      601 atttggcttt tggcacatat ggagggggat cgttatccga aagaatgaga atcacttcag
      661 aaggtaatgt cggtatcgga accgtatctc ccggagccaa attaaatgtc gccggcggcg
      721 atattaaaat gggcgacgcc tcaaacccga gaggacttaa tattctgcaa cattatttat
      781 ctttgggcga aacggaaagc ggagcgagca ccatcttggg aaacaacatt aaagtcaccg
      841 acggagtaga cgaaaccgtt caagtgagcg cttccgccgc cgacggcccc acttatctca
      901 ggttgaatta ttccaatggc attactttcc accgaatcgc tccggcggct gtcggcaccc
      961 aatatgcttc ttcgaccggg gaattgatga gaataacgac ggccggaaac ttaggtatcg
     1021 gcaccaccgt ccccgcagcc aaactcgcca taaacggcgg tctccacgtc ggcggcgact
     1081 ccgaccccgg agataataat gccttgatcg acggcacctt aaccgtcacc ggaaactcca
     1141 atacctgcaa tatggtttct tacaccggag gcggaggtac cacccagtgt cccgccggct
     1201 tctacacctg gtccggtgtc gccttaactt cgggttacat gctctgctgc aaggtgagta
     1261 atccgatata atgtaaaaga cagcggctgt gtggccgctg tttttgtcga tagattattt
     1321 gttctttttt tccgcttcgt cgagcgcttc ccgggttttc ttttctattt cccggaaaag
     1381 ctttttgtct tcttttaatc tggccttggc cgcttcggtg ccgactccca gtttttcttc
     1441 cccgaaagaa taagtgtttc cggatttctt gatgattccc tggccaatgg ccacgttcat
     1501 cacgtctcct tcgtatgaaa tgccgtccgc gaacatgatg tcgaattcgg ctgtcttgaa
     1561 aggcgccgcc actttgtttt tggccacttt ggcccggacg cggttgccga ccacttcttc
     1621 tccctttttg atctgagcga tccttcggag atcgatcctg atcgtcgaat agaatttcaa
     1681 ggcccggccg ccgggagtgg ttgtcggttc gccgtagccc atcatcccga ttttctcccg
     1741 aagctggttg ataaaaatga tcaaggtttt ggacttgttg gcgatggccg tgatttttct
     1801 catggcctgg ctaatcattc tcgcctggag tccgatgaat tggtctccca tttctccctc
     1861 gatttccgct ttcggggtga gagcggccac ggaatcgacg acgataacgt cgatcattcc
     1921 ggacctgacc agactttcca taatattcag ggcttcttcg ccgctgtccg gctgtgagag
     1981 aagaagttcg gaaaccttaa ccccgatttt tttggcgcga tccggatcga gcgcgtgctc
     2041 ggcgtcgata aaagcggctt t
//
LOCUS       DAOPQT010000103         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_59033_length_2054_cov_3.320160, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000103 DAOPQT010000000
VERSION     DAOPQT010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59033_length_2054_cov_3.320160"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..205
                     /locus_tag="PKG74_02770"
     tRNA            134..205
                     /locus_tag="PKG74_02770"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:166..168,aa:Thr,seq:cgt)
     gene            228..938
                     /locus_tag="PKG74_02775"
     CDS             228..938
                     /locus_tag="PKG74_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOB90225.1"
                     /translation="MDSSPNQTITVSVIPGVGGDDAKDWARMLVRMYESYAKKRGWKS
                     REIHDNVVEIEGAVAYDTLKNESGVHRLVRISPYDAKKLRHTSFALVEVLPELKKFEA
                     DKIQIPESDLKTEFFRSSGPGGQNVNKVETAVRLVHLPTGLVAASQAERSQIQNKERA
                     LILLKSKLAKLMADRRETTLDNLRVKVQPEWSRQIRSYTLHPYKLVKDHRTKAESRRA
                     DEVLEGDLDVFIQAELKL"
     gene            952..1632
                     /gene="ftsE"
                     /locus_tag="PKG74_02780"
     CDS             952..1632
                     /gene="ftsE"
                     /locus_tag="PKG74_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOB90226.1"
                     /translation="MITFSNVSKAYNNHEVLRDISFKIEPKEFVSLVGRSGAGKSTLV
                     RLLIGEDKPTKGRIVFGPYEINKLQSHELPEVRRRMGIIFQDFKLLPKKTAYENVVFA
                     MEVAGRSEEDIDEAAPQALELVGLGKKMRNYVHELSGGERQRVAIARAMVNQPDVIIA
                     DEPTGNLDPVNTWGIINLLLKINELGTTVVLATHNKDIVNQLEHRVISLDDGMVIRDE
                     DKGKYILT"
     gene            1641..>2054
                     /locus_tag="PKG74_02785"
     CDS             1641..>2054
                     /locus_tag="PKG74_02785"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOB90227.1"
                     /translation="MFTTLARIIKYGFQNFWRNGLVSFATILVLLLAVIVFQGMMLFG
                     EIGRQAIDAVQDKIDISVYFVSNAPEDEILRLKEALLTLSEVKSVDYVSKEEALELFK
                     ERHADDPTISQAIEELEENPLRASLNIKAQDPNQYS"
BASE COUNT          565 a          521 c          519 g          449 t
ORIGIN      
        1 gacggagaag cttaagccgg aaagaatctt tatttttccg aaagaaattg acaggttttt
       61 gacttcgaga atgtcggcca ttgatgatat aatacgttta tattactcta tattggcttc
      121 gaataaaact tgtgccggag tagctcagtg gcagagcaac tgtttcgtaa acagtgggcc
      181 gcgagttcga ttctcgcctt cggctccgca aaccaaataa tcatcttatg gattcatcgc
      241 caaaccaaac aataaccgta tccgttattc ccggagtggg cggagacgac gccaaagact
      301 gggcgcggat gctcgtcaga atgtatgaaa gctacgctaa aaaacgcggc tggaaatcgc
      361 gcgagatcca cgacaatgtc gtcgagatcg aaggagcggt agcctacgat accctgaaaa
      421 acgaatccgg cgtccatcgg ctggtcagga tttctcctta cgacgccaag aaattaaggc
      481 acacttcctt tgctttggtg gaagttttgc ccgagctcaa aaagttcgaa gcggacaaaa
      541 tccaaattcc ggagagcgac ctaaaaaccg agttttttag atcgtccgga cccggaggcc
      601 aaaacgtcaa caaagtggaa accgcggtgc ggctggtcca tttgccgacc ggactcgtgg
      661 ccgcctccca ggccgaacgc tcccaaatcc agaacaagga aagggctttg atccttttga
      721 aatccaaatt ggccaaactg atggcggatc gtcgggaaac gaccctcgat aacttgaggg
      781 tcaaggttca gcccgaatgg agccggcaga tcagatcgta taccctgcat ccttataaac
      841 tggtcaaaga ccacaggacc aaagccgagt caaggcgggc ggacgaagtt ttggaaggcg
      901 accttgatgt ctttatccaa gccgagttaa aattataaac aaccaaacca agtgattaca
      961 ttcagcaatg tttccaaggc ctacaacaac cacgaggtct taagggatat tagtttcaag
     1021 atagaaccca aggaattcgt ttctttggtc ggtcgctccg gagccggaaa atcgaccctg
     1081 gtcaggcttt tgataggcga agacaagccg accaagggcc ggatcgtttt cgggccgtac
     1141 gaaatcaaca aactccaaag ccacgagttg ccggaagtga gacggcgcat gggaatcatt
     1201 ttccaggatt tcaagctttt gcccaagaaa accgcctacg agaacgtggt tttcgccatg
     1261 gaagtggctg gccgttccga agaggacatc gacgaggccg ctccccaggc tctggagctt
     1321 gtcggattgg gcaagaaaat gcggaattac gtccatgaac tctccggcgg tgaacgccaa
     1381 agggtggcga tcgcgagggc gatggtcaac cagccggatg tcatcatcgc cgacgagccg
     1441 accggaaacc tcgatccggt gaacacctgg ggcatcatca acctgctttt aaaaatcaac
     1501 gagctcggca cgaccgtggt tttggccacc cacaacaagg atattgtgaa tcagctcgaa
     1561 caccgcgtca tcagcctcga cgacggcatg gttatccgcg acgaagataa aggaaaatac
     1621 atactgactt aattccaata atgtttacca ctttagctcg gatcatcaaa tacggcttcc
     1681 aaaacttctg gagaaacgga ttggtctctt tcgccaccat cctcgtgctt ttgctcgctg
     1741 tcatcgtttt ccagggaatg atgcttttcg gggagatcgg ccggcaggcg atcgatgctg
     1801 tccaggacaa aatcgacatc agcgtttatt tcgtctcgaa cgctccggaa gacgaaattc
     1861 tcaggctgaa agaagctttg ctcactttgt ccgaggtgaa atccgtggac tacgtttcca
     1921 aggaagaagc tctcgagctt ttcaaggaac ggcacgccga cgatccgacc atttcccagg
     1981 cgatcgaaga actcgaagaa aatcccttgc gcgcttcttt gaacatcaag gcccaggatc
     2041 cgaaccagta ttct
//
LOCUS       DAOPQT010000104         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_59059_length_2053_cov_3.531532, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000104 DAOPQT010000000
VERSION     DAOPQT010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59059_length_2053_cov_3.531532"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /gene="mraW"
                     /locus_tag="PKG74_02790"
     CDS             complement(<1..491)
                     /gene="mraW"
                     /locus_tag="PKG74_02790"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HOB90228.1"
                     /translation="MDLTKNTEEKNKSTPIAAHTPVLLNETIRALDPQPGEFFVDGTA
                     GEAGHMRAILEKIGPNGKYLAIDWNKKNVERLAREFADDSRVTVLNMNFADIPEALRG
                     KKADGLLLDLGFSSGELESGRGFSFQKDEPLLMTYSDDQEPLYKILKRLSEKEIFEIL
                     KLTG"
     gene            complement(565..996)
                     /gene="mraZ"
                     /locus_tag="PKG74_02795"
     CDS             complement(565..996)
                     /gene="mraZ"
                     /locus_tag="PKG74_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOB90229.1"
                     /translation="MFIGEYKHNLDTKSRLAIPAKFRNQLSSGAIITRGLDRCLFVFT
                     SEDWDILAQKLVALPLTQADSRAFVRLMLAGAAECDFDSQGRVLIPDYLRKYAELEKE
                     TVVTGLYNRIEIWDSEVWSSYKKKTEGASEEIAEHLRDLGI"
     gene            complement(1188..>2053)
                     /locus_tag="PKG74_02800"
     CDS             complement(1188..>2053)
                     /locus_tag="PKG74_02800"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HOB90230.1"
                     /translation="PDTDSICSAIVYANLKGYIPVRQGELNKETAFALDYFKVPAPQI
                     LESGEGKKLVCVDHNEASQMIENWDNAEIIEVIDHHKINFNYGTPIYFHSEPIGCTGT
                     IIAKQNLEAVKANPVLAGLLLSAILSDTVIFKSPTATEEDKAIATELAKVAGIEDMIA
                     FGIELKTKNADVNGRSIEEIIASDFKDFDFNGKKIGIGQIEVVESAEIESKAKGIIDS
                     LQSLKQSGYEAVFFMATDIMKEGTKLFFVGDPEVVEKAFGVKPENNAIYLPGVMSRKK
                     QIVPNLEKAFG"
BASE COUNT          454 a          542 c          458 g          599 t
ORIGIN      
        1 ccggtaagct tcagtatctc gaaaatctct ttctcggaca gccgcttaag gatcttataa
       61 agcggctcct ggtcgtcgct gtaggtcatg agcaggggct cgtctttctg gaagctgaat
      121 ccccgtccag actcgagctc tcccgaagaa aatccgagat cgaggagcaa tccgtcggcc
      181 ttttttccgc gaagcgcttc tggaatgtcg gcgaaattca tgttgagcac cgtcacccgg
      241 ctgtcatcgg cgaactcccg ggccaggcgt tcgacattct ttttattcca atcgatcgcc
      301 agatattttc cgttcgggcc gattttctcg agaatcgctc tcatatgtcc ggcttctcca
      361 gccgtgccat cgacaaagaa ctctccgggt tgcggatcca aagcgcggat tgtttcattt
      421 aaaagaaccg gggtgtgcgc cgctatcggc gttgacttgt ttttttcttc ggtgtttttt
      481 gttaaatcca tttttcgacc ttttttccga acgatctatt tctcttaggt ttcggcggac
      541 ttgctcctta aagcccgccg aaacctatat ccccaaatcc ctcaaatgtt cggctatttc
      601 ctcggaagcg ccttcggtct tcttcttgta ggaagaccaa acttccgaat cccaaatctc
      661 gatccgattg taaagtccgg tgaccacggt ttctttctcc aattccgcgt atttgcgcaa
      721 ataatccggg ataagcaccc ggccctggga atcgaaatcg cactcggccg cgccggcgag
      781 catcaaacgc acgaaagccc gcgaatccgc ctgggtcaaa ggcagagcca ccagtttttg
      841 cgccagaata tcccaatcct cggacgtaaa aacaaacaaa cagcgatcca ggcctctcgt
      901 gattatggcg ccgctggaca attgattcct gaacttggcc ggaatggcca gccgggattt
      961 cgtgtctaga ttgtgcttat attctccgat aaacatttgg gtttttaagt tatccacttt
     1021 tccccacttt tataccattt tactccacag gcctttttag gtcaaaatgt gtataactcc
     1081 ggctattgtg gaaaagtggt ttattagcaa ctgatccgcc ctgccggagg cgaccaccaa
     1141 aaaagtccca cttctgcggg acttttttgg ttcttctagc ctcttgatca gccgaatgcc
     1201 ttttccagat tgggcacgat ctgtttttta cgagacatca cgcccggcag ataaatagcg
     1261 ttgttttccg gttttacgcc gaacgctttt tcgacaactt ccggatctcc aacaaagaaa
     1321 agctttgttc cttctttcat gatgtcggtc gccatgaaaa ataccgcttc gtatccactt
     1381 tgcttcaaag attgaaggga atcgatgatt ccctttgctt tggattcgat ttccgcggat
     1441 tcgaccactt cgatctggcc gatgccgatt ttctttccgt tgaaatcgaa atccttgaaa
     1501 tccgaagcga taatctcctc tatgctccgg ccgttcacat ccgcgttttt ggttttcaat
     1561 tcgattccga aggcgatcat atcctcgatt ccggcgacct tggcgagctc ggttgcgatc
     1621 gctttatctt cttcggttgc ggtcggcgac ttaaaaatca ccgtatccga caaaatggcg
     1681 gaaagaagaa ggcctgctaa aacaggattc gctttcaccg cttccaagtt ttgtttggca
     1741 ataatcgtcc ctgtgcagcc gatcggctcc gaatggaaat aaatcggggt tccgtaattg
     1801 aaatttattt tgtggtggtc gatgacctcg atgatttccg cattgtccca attttcgatc
     1861 atctgggaag cttcattgtg gtcgacgcag accaatttct ttccttctcc gctttccaaa
     1921 atttgcggag ccggaacttt gaaataatcc agagcaaagg ccgtttcctt gttcaattct
     1981 ccttgtctta cgggaatata gcctttgaga ttggcgtaga caatggccga acagatactg
     2041 tccgtatccg ggt
//
LOCUS       DAOPQT010000105         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_61545_length_1990_cov_2.612920, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000105 DAOPQT010000000
VERSION     DAOPQT010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61545_length_1990_cov_2.612920"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKG74_02805"
     CDS             complement(<1..680)
                     /locus_tag="PKG74_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90231.1"
                     /translation="MTKPLVRPWQARPFRRLSIPLNNPLLLTSLDKQAKALRKIFPEL
                     KDATFDRELAKRIAKWSREVGVGGLFLLPRWELIAAKYNEALLQTWVGAKMAGWSSSS
                     YLGDPEKFCEAVEERFLRQGRFKEGFIRDLAEIQPDSGLLVLPVQPVTPECLAGGMRR
                     ELRSRQGVPLGLFEVLCVIVSQWPDVSYLQCPHMITTSGDLYAKNGDGKFRYAEDSDN
                     MVYIGGLHP"
     gene            complement(827..>1990)
                     /locus_tag="PKG74_02810"
     CDS             complement(827..>1990)
                     /locus_tag="PKG74_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOB90232.1"
                     /translation="IAVGRLYSGVLKKGAWVSHINRRGEIKKVQIANVMLFDGLNRID
                     VPEAVAGEIVAVSGIPEISIGETIAALENPKALPIIHIDEPTIKMTFGVNTSPFVGRE
                     GEYTTSRNLRERLIKELETDVALKVEETDKADRFIVSGRGELHLAILIEKMRREGYEL
                     EVSKPQVIFKENESGVTEPIETVSIEVPEEYAGVIIEMMGKRLAKMKDMNIDNHTMKM
                     DFEIPTRGLIGVRNEFMTSTKGSGIMNTLFSGYAPYKGKLAESTHGSIITTETGITNN
                     YGLVSAQGRGKLFMGAGVEVYEGMVIGENAKSRDELVNVCRQRELTNFRAKNDGLQDQ
                     LEVPLKLTLEDALDYIHDDELVEITPKSIRIRKRYLTENDRRKAAKEKKTEAR"
BASE COUNT          439 a          540 c          453 g          558 t
ORIGIN      
        1 ggatggagac cgccaatata gaccatatta tcgctatctt ccgcgtaacg gaatttccca
       61 tctccgtttt tcgcgtatag gtcgccggaa gtagtaatca tatgcggaca ctgcagatag
      121 cttacatctg gccactgaga gacaataacg cacaaaactt cgaataggcc caatggaacc
      181 ccttgtctac tacgcagctc tcttctcatc ccacccgcca gacattccgg tgtgacaggt
      241 tgcaccggca agactagaag accgctgtca ggttgaattt cagccaagtc tcttatgaaa
      301 ccctccttaa accggccctg gcggagaaat ctttcttcca ccgcctcaca gaacttctcg
      361 gggtctccta gatagctgct ggacgaccaa ccggccatct ttgccccaac ccaggtttgg
      421 agaagagctt cgttgtattt tgccgcgatg agttcccatc ggggaagtag aaacaatcct
      481 cccacgccaa cttctcgaga ccacttggcg atacgcttag ccaactccct atcgaaggtc
      541 gcgtctttca gctccggaaa gatttttcga agagctttcg cctgtttgtc caaagacgta
      601 agaagcaaag gattgttcaa cggaatcgaa agacgacgga aaggccgcgc ttgccatggt
      661 cttaccaacg gtttcgtcat ttatatcatc ctcctttttc ttaaggtgct ccactctatt
      721 agagagaata aaaaatctat tgtcaaatat aaaaacttcc atgtgagaag gcggtttttt
      781 atggtcttcg ccgtctattt cataaactct aagcgtttga atcgatttat cgagcctccg
      841 ttttcttctc ctttgccgct ttgcggcggt cgttctcggt caaatatctt ttcctgattc
      901 taatactctt aggagtgatt tccacgagct cgtcgtcatg gatgtaatcg agggcgtctt
      961 ccaaggtaag cttcaaaggc acctcgagct gatcctgcag gccgtcgttt ttggcgcgaa
     1021 agttcgtcaa ttccctttgc cgacagacgt tcaccaactc gtcgcgggat ttggcgttct
     1081 ctccgatgac catgccttcg taaacttcca cccccgctcc cataaagagc tttccccttc
     1141 cttgggcgct taccaatccg taattattgg tgattccggt ctcggtcgtg ataatcgaac
     1201 cgtgagtgct ttccgccagt tttcctttat aaggcgcgta tccggaaaaa agagtattca
     1261 tgattcccga tcccttggtg ctcgtcatga attcgtttct tacgccgatc aggcccctcg
     1321 tcggaatctc gaaatccatc ttcatcgtgt ggttgtcgat attcatatcc ttcatcttag
     1381 cgaggcgttt gcccatcatc tcgataatca cgcccgcgta ttcctccggg acttcgatgg
     1441 aaacggtttc gatcggctcg gtgacgccgc tctcgttttc tttgaaaatc acttggggtt
     1501 tggaaacttc gagttcgtat ccctcgcggc gcattttttc gatcaaaatg gcgaggtgca
     1561 gttcgccgcg gccggaaaca atgaagcgat cggccttgtc cgtttcttcc actttgagag
     1621 cgacgtcggt ttcgagctcc tttatcaatc tctcccttaa atttcgcgaa gtggtgtatt
     1681 ctccttcccg gccgacaaaa ggcgaagtat tcactccgaa agtcattttg atggtcggct
     1741 cgtcgatatg gattatcggc aaggctttcg ggttttccaa agcggcgatg gtttcgccga
     1801 tcgagatctc cggaatgccc gaaacggcga cgatttctcc ggccacggct tcgggaacgt
     1861 cgattctgtt caagccgtcg aaaagcatga cgttcgcgat ttggactttt ttgatttcgc
     1921 cgcggcgatt gatgtgactg acccacgcgc cttttttcaa aactccggaa taaagccgac
     1981 cgacggcaat
//
LOCUS       DAOPQT010000106         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_62563_length_1966_cov_3.032444, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000106 DAOPQT010000000
VERSION     DAOPQT010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62563_length_1966_cov_3.032444"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..419
                     /locus_tag="PKG74_02815"
                     /pseudo
     CDS             78..419
                     /locus_tag="PKG74_02815"
                     /inference="COORDINATES: protein motif:HMM:NF021158.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
     gene            471..824
                     /locus_tag="PKG74_02820"
     CDS             471..824
                     /locus_tag="PKG74_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOB90233.1"
                     /translation="MLVASDRNLSIFDLDGKRLKMVSFNGKDAPLDPAYTLEFNNTLV
                     SAVICNNIAGDFTLSDNTIKGLFVTTEKFCTSPDGIMEAEDAFKAMLSSGATLFLAGN
                     TLTMSDSENIMVFSQ"
     gene            990..>1966
                     /locus_tag="PKG74_02825"
     CDS             990..>1966
                     /locus_tag="PKG74_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOB90234.1"
                     /translation="MKISEEKRGDEGSMVSVNLGAFCNQNCVFCLAADGKEVYPRLSR
                     DEAIKVIESYLDEKGKNLLLTGGESTIHPDFVEIIGKVIKDDRVRSLNIMTNGVRLGD
                     KSFFDAVIATDPKKKISFSFSLHGHNPKLAESITRGQKGDFEKTCAAMHYAIQGGYSA
                     DVAIIMVEQNYRHLPDFARFIVDEFPGIGGVSFGYPLLFGNAEINKDFIYAKFSDIAP
                     YLKEALNIFLAKGIRAVTAAGAPVPLCAIPGVEEVSVRPLIEWRRRYIGTATSGLLNS
                     LKDEDTIIPKIKPDQCAECVLNDACVGVLKCYADIFGSDGVIPVTIDNF"
BASE COUNT          530 a          435 c          498 g          503 t
ORIGIN      
        1 ccgctttttt tgccggatat tccggaaaaa atgttatact tacaatacag gtcgtcatta
       61 ataataaatt agaaccaatg aataaaaaga ctttatggtc gattgtcatt ctggtcgttg
      121 tggcaatcgt cgtctatttc ggggtggttg tttggcttaa ccggaataac gcggagctta
      181 agggaacggt ttcttaccgc gaacgcattg ctttgccttc ggggagcgag gtaaaagtgc
      241 agatacgtta cgtttctaaa ggagaaacct ttgcgtaaac aagcatcatc acgagagggg
      301 aaaacgtgcc gattccgttc acggttatct acgacgcttc caagatagac ccgagcgctg
      361 attatgacgt ggtggctcaa atccttgtcg acggcgaaga gcgttggacc gtcggctccc
      421 cggaaccgtt cataggcgaa gattcgctgc cggtcgagga aatcgatttc atgctggtgg
      481 cttcggaccg aaacctttcc atcttcgatc tcgacggaaa gagattgaaa atggtctctt
      541 ttaacggaaa ggacgcgcct ttggacccgg cttacacttt ggaattcaac aacactttgg
      601 tctcggccgt gatctgcaac aatatcgccg gagatttcac gctctccgac aacacgatta
      661 aagggttatt cgtgacaacg gagaaattct gcacgagccc ggacggcatc atggaggcgg
      721 aagatgcgtt taaagccatg ctaagctctg gagcgacatt gttcctcgcg ggcaacacat
      781 tgaccatgtc cgatagcgaa aatatcatgg ttttctccca atagtctttt cgcaaacaaa
      841 aaaagaagat aaaacctccg gctacgctgg aggtttttga tataataaaa atcaagagag
      901 tgatcgtgcc atgccttgtc ttggtcggca ttatcggaat atcgattgtc tgaaagattt
      961 tacagaaaga gttataattc aatcgattga tgaagatttc cgaagaaaaa agaggagacg
     1021 agggatcgat ggtcagcgtc aatttgggag ctttttgcaa ccagaattgc gttttttgtc
     1081 tggccgccga cggcaaagaa gtatacccga gattatcccg cgacgaagcg atcaaggtta
     1141 tcgagagtta tcttgacgag aaaggtaaga atttgcttct tacgggcgga gagtcgacta
     1201 ttcatccgga ctttgtggaa atcatcggaa aggtgattaa agacgaccgc gttcgttctt
     1261 taaatatcat gacaaacggc gtcaggcttg gagacaaaag tttttttgac gccgtgattg
     1321 cgaccgatcc caagaaaaag atttcttttt ctttctctct tcacggacac aatccgaagt
     1381 tggcggaaag catcacgcgg ggacaaaagg gagatttcga gaaaacttgc gctgccatgc
     1441 attacgccat ccaaggtggc tatagcgcgg atgtggctat catcatggtt gagcagaatt
     1501 accgtcatct tcccgatttc gcgaggttta tcgtcgatga atttccagga atcggagggg
     1561 tttctttcgg ttacccgctt cttttcggaa acgcggaaat caacaaggat ttcatttacg
     1621 ccaaattcag cgatatcgcg ccttacctca aagaagcctt gaatattttt ctcgctaaag
     1681 gcatcagggc cgttaccgcg gccggagcgc cggtaccgct ttgcgccatc cccggagtgg
     1741 aagaagtgtc cgtaaggccg ctcatcgaat ggagaagacg atatatcgga acggccacaa
     1801 gcggccttct gaattctttg aaagacgagg acacgattat cccgaaaatc aagccggatc
     1861 aatgcgccga atgcgtttta aacgacgcct gcgtcggagt gcttaagtgt tatgccgaca
     1921 tcttcggctc ggacggcgtt atacccgtga caatcgataa ttttga
//
LOCUS       DAOPQT010000107         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_62919_length_1958_cov_1.926432, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000107 DAOPQT010000000
VERSION     DAOPQT010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62919_length_1958_cov_1.926432"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKG74_02830"
     CDS             complement(<1..405)
                     /locus_tag="PKG74_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOB90235.1"
                     /translation="MAAYSKVLKIRLKDKEDIEELAKSGRILRAVLARLRSSVKPGVK
                     TKEIDLLSRELIASAKAKPSFLNYRPEGAIRAFPASVCVSVNETVVHGVPNDYIIKSG
                     DLVKLDLGINLNGYYTDAAVTVIAGKSSPKTKL"
     gene            complement(392..1036)
                     /locus_tag="PKG74_02835"
     CDS             complement(392..1036)
                     /locus_tag="PKG74_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOB90236.1"
                     /translation="MKKQPKVIILFGPPGSGKGTQAAFIKKATKGFALFDGGTEIEKM
                     VRSKKYDDFPGIEAARKAFFSGELVDPLWVIDRLTIPATKKLWKQGKGIIYSGSFRTV
                     LEIFGNKKRTGLLKVVEKLYGKENIIVLQINVPVSESVKRNVKRGRPGLDEAKIIRVR
                     YENYKKLSYPVIQGFKKAGIKVVQIDGKPKREKVWKDIAAALSKLGVEIAVGRL"
     gene            complement(1036..>1958)
                     /locus_tag="PKG74_02840"
     CDS             complement(1036..>1958)
                     /locus_tag="PKG74_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HOB90237.1"
                     /translation="LKYLSAQGGGIIAPMELPALALNLLFIVAGSLIMIWIGEMISEQ
                     KLGDGVSLIIVAGIVSGLPRSVIGQVLMMIGRYQSTGSFLGIGIDKILILLALALVVV
                     AGVVLVNEGERKIPVAYAKRVRGNRVFGGSTTYLPLRVNQAGVIPIIFAISILMLPQY
                     LGLGLRSLDAGWAIKVADFIEATLANPLWNGLLYFVMVFAFTYFYTFIVFEPNEIAKN
                     LQRNGGFIQGIRPGASTAEYLNSIIAKLTLFGALFLGIIAVLPSIVQGITKVANMTIG
                     GTGLLIVVSVALETAKQIDSELKIREYEIA"
BASE COUNT          474 a          518 c          478 g          488 t
ORIGIN      
        1 caatttggtt ttcggagagg acttgccagc gatcacggta acagccgcat ccgtgtaata
       61 gccgttcaag ttaatgccga ggtcgagctt cactaaatca cctgacttga tgatgtaatc
      121 gttcggcact ccgtgaacca cggtctcgtt tacggaaacg caaacgcttg ccggaaaagc
      181 cctgatcgct ccttcgggcc ggtaattcaa gaaagaaggt ttggctttgg ccgaggcgat
      241 cagttcccgg gaaaggagat cgatctcttt tgttttcacg ccgggcttga ccgaagatct
      301 caaacgcgcc aaaaccgccc tcaaaatcct gccggatttg gcgagttcct cgatatcctc
      361 tttgtcttta agcctgattt tcaaaacctt gctataggcg gccaaccgcg atctccactc
      421 ccaatttcga aagcgccgcg gcgatatcct tccaaacttt ttcccgtttc ggtttgccgt
      481 cgatttgaac gacttttatg ccggcttttt tgaagccttg gataaccgga tagctcaatt
      541 ttttgtaatt ctcgtatctc acgcggatga tcttggcttc gtcgagcccg ggccggccgc
      601 gcttcacgtt tcttttgacc gattcggaaa ccggaacatt gatctgaagg acgatgatgt
      661 tttcctttcc gtaaagtttt tccacgactt tcaggagccc tgttcgcttt ttgttcccga
      721 aaatctcgag cacggtgcgg aaagaaccgc tgtaaatgat tccctttcct tgcttccaaa
      781 gtttcttggt cgcgggaatg gtcagccggt cgatcaccca aagaggatcg acaagctcgc
      841 cggagaaaaa agcttttctc gcggcttcga tacccggaaa atcgtcgtat tttttcgacc
      901 gcaccatttt ttctatctcg gttccgccgt cgaacaaagc gaatcctttg gtcgcttttt
      961 taatgaaagc cgcctgagtc ccttttcccg agcccggagg cccgaacaaa ataatgactt
     1021 tgggctgttt tttcattagg cgatttcgta ttccctgatc ttcagctccg aatctatctg
     1081 tttggcggtc tcgagagcca cggaaacgac gatcaaaaga ccggtgccgc cgatcgtcat
     1141 gttggcgacc ttggtgatcc cttggacgat cgaaggaaga acggcgatga tgccgaggaa
     1201 gagtgctccg aacaaagtca gtttggcgat gatcgagttc aggtattcgg cggtggaagc
     1261 acccggcctg atgccctgga taaaccctcc gttcctttgg agatttttgg ctatttcatt
     1321 cggctcgaaa acgatgaagg tgtaaaaata agtgaaggcg aaaaccatga caaaataaag
     1381 gaggccgttc caaagtggat tggcgagagt ggcttcgatg aaatcggcta cctttatcgc
     1441 ccagcccgcg tccaaagacc taagtcccag tccgagatac tgcggcaaca tcaatatgga
     1501 aatggcgaag atgatcggaa taactccggc ttggttcacc ctcaaaggaa gataagtggt
     1561 cgatcccccg aaaacccggt tgccccgcac tcttttggcg taagccaccg gtattttccg
     1621 ttcgccttcg ttcaccaaaa cgactccggc gacgacaacc aaggccaaag ccaaaagaat
     1681 cagaatctta tcaatgccta tgccgagaaa actgccggtc gactgatatc ggccgatcat
     1741 catcagaacc tgtccgatga cggaacgcgg caagccgcta acgattccgg cgacgataat
     1801 cagggaaact ccgtctccca atttctgttc gctgatcatc tctccgatcc agatcatgat
     1861 gagcgatccg gcgacgataa aaagcagatt caaagccagg gccggcaatt ccatgggagc
     1921 gatgataccg ccgccctggg ccgaaagata tttgagaa
//
LOCUS       DAOPQT010000108         1946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_63393_length_1946_cov_3.221576, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000108 DAOPQT010000000
VERSION     DAOPQT010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63393_length_1946_cov_3.221576"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKG74_02845"
     CDS             complement(<1..226)
                     /locus_tag="PKG74_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90238.1"
                     /translation="MSYENPLFVAGIAVASLALGYLIRQSLGIRFLRQEADKKAKEAL
                     EAADLEAKNLVLKAKDRAVFIVEEAKREEKE"
     gene            289..1815
                     /gene="gpmI"
                     /locus_tag="PKG74_02850"
     CDS             289..1815
                     /gene="gpmI"
                     /locus_tag="PKG74_02850"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOB90239.1"
                     /translation="MSKKTGFKKVVLVVLDGWGVGPKDRSNPIWTANPQSIAYIKENF
                     PLVSLQASGIAVGLPWQEEGNSEIGHLTIGSGRVIYQPFPKITMAVKDGSFFQNKVLL
                     DAVNYARENKVGLHFVGLFGEANVHSSLEHLQALVKMATDNGFPDFRLHLITDGRDGP
                     PEAGLKLLGQIPQGNISSVSGRFFSMDRDNYLDRTAAGFRAMIGQSENEPDAASFLRK
                     MYSEKITDEFIRPTTIGDPGKSVKNGDAVVFFNFREERMKQLVRMAIDNLPDSRLVSF
                     VEYDPSFKIPVAFPAEKVEQPLAKVLSDRGLTQLRIAESEKEAHVTFFFDGQKTEAYP
                     NEYRVIIPSRKTANHALFPEMMSEEITARATTAIEEGIYNFILINYANPDMVAHTGDF
                     EACLKAVASIDKSLRALVDAGLKTDTPLIITADHGNIERLLNPHTGEPETKHDPNPVP
                     FYLVSEAFRQPKTPVEADQREKEVAGSLADIAPTILELMDIPKPTEMTGESLLSYLNS
                     "
BASE COUNT          496 a          557 c          452 g          441 t
ORIGIN      
        1 cctctttctc ttctcttttg gcttcttcga ctatgaaaac agccctgtct ttggctttca
       61 aaacaaggtt tttggcctcc aaatccgcgg cttcgagcgc ttctttagct tttttgtccg
      121 cttcctgcct taaaaatcga attcctaaac tttggcgaat aagataccct aaagcaaggc
      181 tcgcaaccgc aatcccggcc acaaaaagtg gattttcgta actcatgtat taagattaat
      241 gtattagatt gcatgacctt ggctaatcat aactcggaaa gaaacgcggt gtcaaaaaaa
      301 acaggcttca aaaaagtcgt cttggtggtt ttggacggct ggggcgtcgg ccctaaagac
      361 cgctccaacc ccatctggac ggccaacccg caatcgatcg cctacatcaa agagaacttc
      421 cccttggtca gccttcaggc ttcgggcatc gccgtcggac tcccctggca agaagaagga
      481 aacagcgaga tcggccatct cacgatcggc agcggccggg tgatttacca gcctttcccg
      541 aagatcacca tggccgtgaa agacggtagt tttttccaaa acaaggttct tctcgacgcc
      601 gtaaactacg ccagagaaaa caaagtcggt cttcattttg tcgggctttt cggagaagcc
      661 aatgtccatt cttctttgga gcatctccag gcgctcgtca aaatggcgac ggataacgga
      721 ttccccgatt tccggctgca tctcatcacc gacggacgcg acggccctcc cgaagccggt
      781 ctgaagcttt tgggacaaat tccccaagga aatatttcga gcgtttccgg aagattcttc
      841 tccatggacc gcgacaatta tctcgatcgg accgccgccg gattcagggc gatgatcggc
      901 caatcggaaa acgagccgga cgcggcgtct ttccttagaa aaatgtacag cgagaaaatc
      961 accgacgagt ttatccggcc gacgacgatc ggcgacccgg ggaaaagcgt caaaaacgga
     1021 gatgctgtcg tttttttcaa tttccgcgaa gagcgcatga aacagctcgt cagaatggcg
     1081 atcgacaatc tcccggattc ccggctcgtt tcctttgtcg aatacgatcc ttctttcaag
     1141 attccggtgg ctttcccggc ggagaaagtc gaacagccgc tcgccaaagt tttgagcgat
     1201 cgcggtctca cccagctgcg gatcgccgaa agcgaaaaag aagctcatgt cactttcttt
     1261 ttcgacggcc agaaaaccga agcctacccg aacgaatacc gggtcatcat tccttcgcga
     1321 aaaacggcga atcacgcgct ttttccggaa atgatgtctg aagagatcac cgccagggcg
     1381 accacggcca tcgaagaagg aatctataac ttcattctca taaattacgc caatccggac
     1441 atggtcgccc acaccggaga tttcgaagcc tgcctcaaag ccgtggcctc gatcgacaaa
     1501 agtctccggg ctcttgtcga cgccggtctc aagaccgata ccccgctcat catcaccgcc
     1561 gaccacggca acatcgaacg gctgctaaac cctcataccg gcgagccgga aaccaagcac
     1621 gacccgaacc cggtgccgtt ctaccttgtt tccgaagctt ttcgtcagcc gaaaaccccg
     1681 gttgaggccg accaaaggga gaaagaagtc gccggcagcc tggcggacat tgctccgact
     1741 attttagaat taatggacat tccgaaacca acggaaatga ccggagaaag cctgctttct
     1801 tatctgaatt catgaccaaa ctgggaaaat tgatttttct cttcctcata aacgggttgg
     1861 ggctttattt gagccaccga tttattccgg gattccaaat ttccctcgat cccaaaaccc
     1921 tcgccgaaac cgccggacta ctcacg
//
LOCUS       DAOPQT010000109         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_64985_length_1910_cov_3.291644, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000109 DAOPQT010000000
VERSION     DAOPQT010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64985_length_1910_cov_3.291644"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..344
                     /gene="rpsK"
                     /locus_tag="PKG74_02855"
     CDS             15..344
                     /gene="rpsK"
                     /locus_tag="PKG74_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOB90240.1"
                     /translation="MYIKVSFNNTLVTVTDNHGNVVTWASAGSLGFAGPKKATPFAAQ
                     KIIAAIAEKIAKTGPFNVDVVVSGVSVGRDSAIRSLVNHGFNIMSIKDVTPVPHNGPR
                     PKKPRRV"
     gene            354..968
                     /gene="rpsD"
                     /locus_tag="PKG74_02860"
     CDS             354..968
                     /gene="rpsD"
                     /locus_tag="PKG74_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOB90241.1"
                     /translation="MSRRILEKTERRLGAKLFLKAERCLSAKCAEVKKPYAPGQHGKG
                     RRRQGEYGKQMAEKQKVRFTYGLTEAEMKKAFSKSEQSKEPTDVALVKILESRLDNVV
                     FQLGLAPSRRMASQLVGHGHITVNGRKTTIPSMRVRPGDKIAFRKESADHPVAKAAET
                     RLKQYEPPTWLKLDKEKMIGEMVSEPKDIDLPFDLELVVNFYSK"
     gene            972..1730
                     /gene="rpoA"
                     /locus_tag="PKG74_02865"
     CDS             972..1730
                     /gene="rpoA"
                     /locus_tag="PKG74_02865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOB90242.1"
                     /translation="MKYALLSETVNIKTISEDNKIGKFSVEGLYPGYGLTLGNSLRRV
                     LLSSLPGAAITQFKVKGILHEFSTIPGVVEDVVEIGLNLKKIRFQFFADEPQVLTLKI
                     KGEKDVVAGDIEASTQVVVMNPEEHLFTVTDKKADVEMEITVDKGLGYVPVERRKTEK
                     LEIGVVALDAIFTPVQAVSFKVDNMRVGDRTDYNKLDLEIVTDGTITPSDALHKAGNI
                     LLDHFKKISEISVRELSVEKSGKKKSSAREKKEE"
     gene            1733..>1910
                     /locus_tag="PKG74_02870"
     CDS             1733..>1910
                     /locus_tag="PKG74_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HOB90243.1"
                     /translation="MHHGKKGRKFGRKTGDRRQFIKGLLNNLIIRESIKTTEARAKEI
                     KPLAEKLVTLAKKQD"
BASE COUNT          558 a          458 c          517 g          377 t
ORIGIN      
        1 tcgactccgg caagatttac atcaaggtgt ctttcaataa caccctcgtt accgtgacgg
       61 acaaccacgg caacgtcgtc acctgggctt cggccggatc cttggggttt gccggtccga
      121 agaaggcgac gcctttcgcc gctcagaaaa taatcgccgc cattgccgag aagatcgcca
      181 agaccgggcc tttcaacgtc gacgtcgtcg tttccggcgt ttccgtgggt cgggattcgg
      241 ccatcaggtc gctcgtgaat catggtttca atattatgtc gatcaaagac gtcacaccgg
      301 tgccgcacaa cggtccgcgt ccgaaaaaac cgagaagagt ctaattcaaa aaaatgtcca
      361 gaagaatttt ggaaaaaaca gaaagaaggt tgggggcgaa gctttttttg aaagccgagc
      421 gttgcttgtc ggcgaaatgc gccgaagtga aaaagcctta cgctccggga cagcacggca
      481 aaggccggag acgtcaggga gaatacggca aacagatggc ggaaaagcaa aaagtccgct
      541 ttacctacgg cttgaccgag gctgaaatga aaaaagcttt ctcgaagagc gaacaaagca
      601 aagagccgac cgacgtcgct ctggtgaaga tattggaaag ccggttggac aacgttgttt
      661 tccaattggg gttagcgcct tcgcggagaa tggcgagcca gcttgtcggc cacgggcaca
      721 tcacggtgaa cggccggaaa acgaccattc cttcgatgcg cgtgcgtccg ggagacaaga
      781 tcgctttccg caaggagtcg gccgaccatc cggtggccaa ggcggccgaa acccgcttga
      841 agcaatacga accgccgacc tggctgaaat tggacaagga aaaaatgatc ggcgaaatgg
      901 tctccgagcc gaaagacatc gatctgcctt tcgatttgga attggtcgta aacttttatt
      961 caaaataaaa aatgaaatac gcactactca gtgaaacagt caatatcaaa accatttccg
     1021 aagacaataa aatcggaaag ttcagcgtgg aaggccttta tccgggatac ggcctgactt
     1081 tggggaacag cctccgaagg gtacttttgt cgtctttgcc cggagcggcc atcacccagt
     1141 tcaaggttaa ggggattttg cacgagtttt cgaccatccc cggggtggtg gaagacgtgg
     1201 tggaaatcgg attgaatctg aagaaaatca ggttccagtt tttcgccgac gaaccgcagg
     1261 tgctgacttt gaaaatcaaa ggcgaaaaag acgtcgtcgc cggagacatc gaagcgtcga
     1321 cccaggtggt ggtgatgaat cccgaagagc atctttttac cgtcaccgac aagaaagccg
     1381 atgtcgagat ggaaatcacc gtcgacaagg gattgggata cgttccggtg gagcggagaa
     1441 aaacggaaaa gctggaaatc ggagtcgtcg cccttgacgc cattttcact ccggtgcagg
     1501 cggtgagctt caaagtggac aacatgcgcg tcggcgaccg caccgattac aacaaactcg
     1561 atttggagat cgtgaccgac ggaacaatca ctccttccga cgccctgcat aaagccggaa
     1621 acatcctgct cgatcatttc aaaaagattt cggaaataag cgtcagggaa ttgtctgttg
     1681 aaaagtccgg gaaaaagaaa tcttcggctc gcgagaaaaa agaagaataa tcatgcatca
     1741 cggcaaaaaa ggaagaaaat ttggaagaaa aaccggcgac cgccggcaat tcataaaggg
     1801 tttgctgaac aacctgatta ttcgcgaatc catcaaaacc accgaggcga gggccaaaga
     1861 aatcaaacct ttggcggaaa aactcgtcac tttggcgaaa aaacaggatt
//
LOCUS       DAOPQT010000110         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_65331_length_1902_cov_4.010287, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000110 DAOPQT010000000
VERSION     DAOPQT010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65331_length_1902_cov_4.010287"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PKG74_02875"
     CDS             complement(<1..539)
                     /locus_tag="PKG74_02875"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOB90244.1"
                     /translation="MKLTFLGGVGNMTGSSYLLESEPLDPGKKPTKILIDCGLEQGGS
                     YCEDSNFKPFPFDPKEIDAVVATHSHIDHIGRLPRLVKSGFKGPIISTPPGKDFAEEL
                     LLDSEQLLRNEAEAKRKEPLYNLDDVEKTLGLWQSIRYHEIKKIKDFEVELYDAGHIM
                     GSSFVVVSAMEPKKNNEGVV"
     gene            complement(912..1481)
                     /gene="ruvA"
                     /locus_tag="PKG74_02880"
     CDS             complement(912..1481)
                     /gene="ruvA"
                     /locus_tag="PKG74_02880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOB90245.1"
                     /translation="MIYSLKGKLEGKGSGYVIVETSGVGYKVFFPASSENKLPGIGKE
                     IKLYTELRIKKDEGFDLYGFLYKEERDFFELLNTVSGIGPKTALGILNLASVGDLKAA
                     IADGNKDILVKSIGIGKKTGERLITELKDKIFIPESGKKSAAWDEDVYQALLELGYKS
                     KDAKEAVVKINPNLKTSAERLKEALKKVK"
     gene            1495..1830
                     /locus_tag="PKG74_02885"
     CDS             1495..1830
                     /locus_tag="PKG74_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90246.1"
                     /translation="MTNEELEKFEKKLLQEEAEVEAKIKATPDIIEFGDSQDEDTEEA
                     DESAAADLQASKQDILLHKLDDIRSALNKIKNKTYGKCEIGGEEIEMEILELNPSARY
                     CRKHNKPES"
BASE COUNT          503 a          470 c          437 g          492 t
ORIGIN      
        1 acaacgcctt cattattctt tttcggctcc atagccgaaa cgacgacaaa actcgatccc
       61 atgatatgcc cggcgtcgta aagctcgact tcgaaatcct ttattttttt gatctcgtga
      121 taacggatgg actgccagag tcccagtgtc ttttccacgt cgtccaggtt atagagcggc
      181 tcttttcttt tggcttcggc ttcgttcctc aaaagctgtt cggaatcgag cagtagttcc
      241 tcggcgaaat cttttcccgg aggcgtggaa atgatcgggc ccttgaatcc gcttttgacg
      301 agccgcggca gacgaccgat gtgatcaatg tggctatggg tggcgacgac ggcgtcgatc
      361 tccttgggat cgaaaggaaa cggtttgaaa ttggaatctt cgcaataact gccgccctgc
      421 tccaaaccgc agtcgatcaa aattttggtc ggcttttttc ccggatcaag cggttcgctt
      481 tccaaaagat aacttgagcc ggtcatattt ccgactccgc ctaagaaagt aagtttcata
      541 atatagatta tatcccaaag gttttatcga ataaaaatac ctctagacaa aaggcaacaa
      601 atgtgttata aataaaatat aaactgatct tgaaaggagg aagaataggt ggcttgtcga
      661 gtaacgaaaa tcggcagcgg aagggtcgcc gttgtaacgc ccgagcaaat agaaattgtg
      721 ttttgccttg accccaaaac caagaagccg cttattcttt caaagaccaa ctacggagaa
      781 ggagaagata tgtgggttag tccccgaaac ttctccgaag ctctccgcgt ggtcgctgga
      841 atcttccacg acccaagcag agccgaagct ctgcggaacc catagggggc gacgatatgc
      901 gcgccccttt tctatttcac ttttttaagc gcttctttca gtctctcggc cgaagttttg
      961 aggttcgggt ttattttaac taccgcttcc ttggcgtctt tcgatttgta gccgagctcc
     1021 aaaagcgcct gataaacgtc ttcgtcccag gcggctgatt tcttcccgga ctcgggaata
     1081 aagatcttgt ccttaagctc ggtgatcaat cgctcgccgg ttttctttcc gatgccgatc
     1141 gatttcacga gaatgtcctt gttcccgtcg gcgatggcgg ctttcaaatc tccgacagac
     1201 gccagattca aaatccccag agcggttttc ggaccgattc cggagaccgt attcagaagc
     1261 tcgaagaaat ccctttcttc tttatataaa aatccgtaaa ggtcgaaccc ttcgtctttt
     1321 tttatcctaa gctcggtgta gagttttatt tctttcccta tacccggaag cttgttctcg
     1381 gacgaagccg ggaaaaacac tttgtacccc actccggacg tttccacgat tacgtatccc
     1441 gatcctttcc cctccagttt gccttttaag ctgtaaatca tatactttga ttatatgacg
     1501 aacgaggaat tagaaaaatt cgaaaaaaag ctgcttcaag aagaagccga agtcgaggcc
     1561 aaaatcaaag ccacgccgga catcatcgaa ttcggcgatt cgcaggacga ggataccgaa
     1621 gaagccgacg agtcggccgc ggccgatctc caggcgagca agcaggatat tcttctccac
     1681 aagctcgacg atatccgctc ggctttgaac aagatcaaaa acaaaactta cgggaaatgc
     1741 gaaatcggcg gagaagagat cgagatggaa atactcgaat tgaatccgtc ggcgcgctat
     1801 tgccggaagc acaacaaacc agaaagctga ttcagcagaa gaaaacaaat taaggccgac
     1861 attgtcggcc ttttttattc aagaaattag ttcgtggccg cg
//
LOCUS       DAOPQT010000111         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_65893_length_1891_cov_4.105664, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000111 DAOPQT010000000
VERSION     DAOPQT010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65893_length_1891_cov_4.105664"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="PKG74_02890"
     CDS             <1..1660
                     /locus_tag="PKG74_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HOB90247.1"
                     /translation="ISGLTISGTDAGNYTLTQPTTTASIVASGLTISGVTADDKIYDA
                     TTVAELNVSAAALVGVNPGDDVVLNTANAVGNFSDKNVGSGKTVTTSGFTISGADAGA
                     YTLNQPTTTASITEKTLTVMGITANDKVYDGTTSATLNVSGAALVGVINPDVVFLDTS
                     SATGTFDTPDVGTNKTVTITGLAINGADAGNYTLTQPTTTANITTAPIVATRIVILQP
                     TDATVGDPITVTLRAEDNSGNLAASFDNVVTLLTTGSATGAGPVNIVNGIGSINLNNA
                     IAETVTLSLLDSTGTGLDVSSTRTVSWTNTPPPPSTTSSQSAPPIPRFIGIAFPNAEI
                     FISSFDSRGVFESSRTISSAEGYFDAFLKTEGNTTYSVSSTDRNGDVSITKVFSLDLN
                     IGGLETNLDIIIAPTIRLSRNAVRRGDNLVVSGYAFPNAGVRAFVDGAEKGGIVYPDN
                     SGRYSISINTADIDFGSHSVVVNETFNDGRKSENSIARFFTVSRVFTPQADFNADGKV
                     DIQDWSVFLSRWNPSGEPDLRVDLNGDGKVDIADFSIILRAIQR"
     gene            1669..>1891
                     /locus_tag="PKG74_02895"
     CDS             1669..>1891
                     /locus_tag="PKG74_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90248.1"
                     /translation="MKKSSILLAAVFIAVINIRQASAATFGLSASGESFSVGDNLTVD
                     LDIDSQGVSVNAGQGTIRFSPDILEAESVD"
BASE COUNT          454 a          545 c          474 g          418 t
ORIGIN      
        1 gatctccggt ctcacgattt ccggcaccga cgccggaaac tacactctta cccaaccgac
       61 gacaacggcg agtattgtcg cttccggcct gaccatatcc ggcgttaccg ccgacgataa
      121 aatatacgac gctacgaccg ttgccgagtt gaacgttagc gctgccgcgc ttgtcggcgt
      181 caatcccgga gacgacgttg ttttaaatac cgcgaatgcc gtcggtaatt tttccgacaa
      241 aaacgtcggc tcgggaaaaa ccgtaaccac ttccggattc accatatcgg gagcggatgc
      301 gggcgcatac actctgaatc agccgacgac aacggcgagc atcaccgaaa aaaccttgac
      361 cgttatgggc attaccgcta acgacaaggt atacgacggt acgacgagcg ccaccttgaa
      421 tgtttccgga gcggcgcttg tcggcgtcat caatcccgat gtggtctttt tggacacttc
      481 ttccgcgacc ggaacattcg acacgcccga tgtcggaacg aacaaaaccg ttaccataac
      541 cggtcttgcc ataaacggcg ccgacgccgg aaactatacc cttactcagc cgacgacaac
      601 ggcaaacatc acaaccgctc ccattgtcgc tacgagaatc gtcattctgc agcctaccga
      661 cgcaacggtc ggcgatccca taacagtcac tttgcgcgcg gaggataatt ccgggaactt
      721 ggccgcctcg ttcgataacg tcgtcaccct gctcaccacc ggttcggcga ccggagccgg
      781 tccggtcaat atcgtaaacg ggatcggttc gatcaatctc aacaatgcca tcgccgaaac
      841 cgtcacctta tccttgctcg attccaccgg aacaggactc gacgtttctt cgacgagaac
      901 cgtttcctgg acaaacaccc ctcctcctcc gtccacgact tcttcccaat cggctccgcc
      961 gataccgaga ttcataggta tcgcttttcc gaacgccgag atttttatct cttctttcga
     1021 ctcccggggg gtgttcgaat cttcccggac catttcttcc gccgaaggat atttcgatgc
     1081 ttttctgaaa accgaaggaa acaccaccta tagcgtgtcg tcgaccgaca ggaacgggga
     1141 tgtttccatc accaaggtct tcagcctgga tctcaatatc ggaggattgg aaactaattt
     1201 ggatatcatc atcgcgccga ccatccgtct ttcccgaaac gccgtgcggc gcggagacaa
     1261 tcttgtggtg agcggctacg cttttccgaa cgccggggtc agagcttttg tcgacggcgc
     1321 ggaaaaaggc ggaatcgttt atccggacaa ttccggcaga tacagcatat cgatcaatac
     1381 ggccgacatc gatttcggca gccattcggt cgtggtcaac gaaactttca acgacggcag
     1441 gaagagcgag aattcgatcg cccgcttttt caccgtttcc cgcgttttca ctccgcaggc
     1501 ggatttcaac gcggacggca aagtggatat ccaggactgg agcgtttttc tttcgagatg
     1561 gaatccgtcg ggagaacccg atttgagggt tgacctgaac ggagacggca aggtcgatat
     1621 cgcggacttt agtataatat taagagcgat tcaaagataa aaatcaaaat gaaaaaatca
     1681 tcaattctat tggcggcggt tttcatagcg gtgatcaata tccgccaggc gtcggccgcc
     1741 actttcgggc tttcggcgag cggagaaagc ttcagtgtcg gagacaactt gacggtcgat
     1801 ctcgatatcg acagccaagg tgtttccgtg aacgccggac agggaacgat ccggttttct
     1861 ccggatatcc tcgaggcgga aagcgtcgac a
//
LOCUS       DAOPQT010000112         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_66066_length_1888_cov_2.327332, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000112 DAOPQT010000000
VERSION     DAOPQT010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66066_length_1888_cov_2.327332"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="PKG74_02900"
     CDS             <1..1722
                     /locus_tag="PKG74_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HOB90249.1"
                     /translation="ERMKPAFQKMAAVGTAGFAALTGAIALSVKETIADEAAMNRLAH
                     ILRTSRDATDEQIESLYKQADALERVGVISAGSIIQAQAQLATFDLQSESIEKLIPSI
                     LDYVVAEKGAAATTEDLRGLTNGLAQALQGNFASLTKTGFVLDDATKALIANGTEAER
                     AAALVDVLNSTYKGFNEAARNTTEGGIIAMNNELNRLRATIGKQFLPIIQEITTKLTP
                     LLTSFISWIEANPELFRTITIVVGGLFALLAVVGLLGLAIPALSAGFADLAVVIGFLV
                     SPIGLIVLGIAALIAIGVLLYKNWDTISAYAVQVWGAIKQTFEDAWNAISAAVSNAWN
                     AIVGAINTGFEWIKTAFWFFIDFVIGWWAMLFDLLVPGWRGALAQLWADASLYFKMIG
                     EYISGVMTSIKTWISDALKAISEVWNSVWGQVKDYFSTIWETIKSVASSAFDFIQQGI
                     DKLLGPLQRLIDMAQSAFNAAKSAVSSVGGSISSGIQSIVNTGKAITGRAGGGPVFAG
                     TPYIVGENGPELFVPGSSGSIIPNGRSAGGPTFQIVITGNTLLDRDSARKIGDQLVRY
                     LKDNIRI"
BASE COUNT          453 a          523 c          499 g          413 t
ORIGIN      
        1 gagaggatga aaccggcatt tcaaaagatg gccgccgtag ggacggccgg ttttgccgcg
       61 ctcaccggcg cgattgctct ttccgtgaag gagacgatcg ccgatgaggc cgccatgaat
      121 cgactcgcgc acatcctccg gacttcaagg gatgcaacgg acgaacaaat tgaatctctc
      181 tacaaacaag ccgatgcgct cgagcgcgtg ggagtcattt cggccggaag catcatacag
      241 gcgcaagcac aactcgcgac gttcgacttg cagtcagaat cgattgagaa actcattccc
      301 tcaattcttg actacgttgt cgcagaaaaa ggcgcggccg ctacgacaga ggacctccgc
      361 ggactcacaa acggcctcgc tcaagcgttg caaggaaatt tcgcgtcgct cacgaagaca
      421 ggattcgtcc tcgacgatgc gacgaaggcc ctcatcgcaa acggcacaga agccgagcgt
      481 gcggcggctc tcgtggatgt cctcaattct acatacaaag gattcaacga ggccgcgaga
      541 aacacgaccg aaggaggaat tatagcgatg aataatgaac tcaaccgcct tcgcgccaca
      601 atcggaaagc aatttctccc gatcattcaa gagatcacga caaaactcac tcccctcctc
      661 acgtctttca tctcatggat tgaagcgaac ccggaacttt tccgcacgat cacgatcgtc
      721 gtcggcggat tgttcgcgct tcttgcggtc gtggggcttc tcggactcgc cattcccgcg
      781 ctctcggccg gattcgccga tctcgcggtc gtgatcggtt ttctcgtttc tccgatcgga
      841 ctgatagtcc tcggcatcgc cgcgcttata gcgatcggcg tgcttttata taaaaattgg
      901 gacacgatct ccgcttacgc ggtccaagtt tggggcgcta tcaagcagac tttcgaggac
      961 gcatggaacg ctatctccgc ggccgtttca aacgcatgga acgcgatcgt cggcgccatc
     1021 aataccggtt ttgaatggat aaaaaccgcc ttttggtttt tcatcgattt cgtgatcgga
     1081 tggtgggcaa tgctctttga tctactggtc ccgggatgga gaggcgctct cgctcaatta
     1141 tgggcggacg cttcgctata tttcaaaatg atcggagaat atatctccgg agtcatgaca
     1201 agtattaaga catggatctc ggacgctctc aaagcgatct ccgaagtttg gaattcagtt
     1261 tggggacagg tcaaagatta cttctcgaca atttgggaaa cgatcaaatc ggtcgcctcg
     1321 tccgcgtttg atttcatcca acagggaatc gataaattac tcggtcccct tcaacgcttg
     1381 atcgatatgg cgcaatcggc tttcaatgcc gcaaagagcg cggtttcgtc cgtcggagga
     1441 tctatttcat cgggcattca gtcgatcgtc aacaccggca aggccatcac cggccgggcc
     1501 ggcggcggac cggtatttgc cgggacgcct tacattgtcg gcgaaaatgg tccggaatta
     1561 ttcgttccgg gatcttccgg atcgatcatt ccgaacggca gatcggccgg cggaccgact
     1621 ttccagatcg tcatcaccgg aaacacactt ctcgatcgcg atagcgcccg caagatcggc
     1681 gaccaactcg tgcgctacct caaggacaac atccgaatat gatctatgtc ttcatcaaaa
     1741 agaaaaccga ggaggacttt gtcgatcgca cggatcaaat aaagtcgatc agcatcacgc
     1801 aaggtctcac gaaggaggcc acgaccgcgg cgctcgtcgt cgactactac ggcgcgaaat
     1861 atcggccgga aggcgaggat gaggtcga
//
LOCUS       DAOPQT010000113         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_68018_length_1848_cov_10.844953, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000113 DAOPQT010000000
VERSION     DAOPQT010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68018_length_1848_cov_10.844953"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(492..579)
                     /locus_tag="PKG74_02905"
     tRNA            complement(492..579)
                     /locus_tag="PKG74_02905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(543..545),aa:Ser,seq:gga)
     gene            693..1028
                     /locus_tag="PKG74_02910"
     CDS             693..1028
                     /locus_tag="PKG74_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90250.1"
                     /translation="MRSDRVQAALRELDRIGGEEMLRGSNDNERLGILEKLLPETCSW
                     PIMPARELIFRIQKEWGIDMDVCPFYFLEDDESETQYCLYGGEKAECLCGQPCEKCVI
                     RDQEEVVLF"
     gene            1121..1339
                     /locus_tag="PKG74_02915"
     CDS             1121..1339
                     /locus_tag="PKG74_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOB90251.1"
                     /translation="MPEKIRKIYRSRTDRIIFGVCGGLGEYFEVDPVIFRLLFVLFAF
                     MASGILIYLISAIIIPLESKKTAKTIED"
     gene            complement(1428..>1848)
                     /locus_tag="PKG74_02920"
     CDS             complement(1428..>1848)
                     /locus_tag="PKG74_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90252.1"
                     /translation="PAEISKLEKAIAKLQEDFETAKEDGEEACRIRDEITKKREELKY
                     LFDLQRCAVLVEKPQNDEVATIGHRVTYHNARNGESRTIVLCGDWTPDEERCRRTRIK
                     TPLFAALEGAKRGEERNLFTNESQRIVIDRIEAADDE"
BASE COUNT          486 a          420 c          439 g          503 t
ORIGIN      
        1 tgtcttccgc ttgggcaaac gcttcaatca attattttga aaatgaaaaa atgaagattg
       61 cccaagcgga aagctctttc gcccaaaaag cgagaaatga gcttgtagag atagaaacaa
      121 aacttgatcg cttgcttgat ttgcaactgg acggcaattt gtcgcaagcg gaatataccg
      181 ctaaaaagcg caagctcatt ctcgctaaaa aagatttgga agaaaaaata tccgcttttg
      241 ggcgaaagag caataatcgg ttcgaacttg ccataaactt cataaaagaa gccaaccaag
      301 ccgaaaaatg cgctcaacaa gaaaatcccg aaaggattcg ggattttctc aaaaagattg
      361 gttcgaactt ccgcattgcc gaccgcactt tgtttttgga tttcaaaaat accttcaaaa
      421 ttgcggaaaa ataccatgcc gaggcgcgaa gcgccgaggc aaactctttc aatttcgctg
      481 aatgtaaaac ttggcggaga ggcagggatt cgaaccctgg ataccttgcg gtatattcgc
      541 tttccaagcg aacgcactag accgctatgc gacctctcca agcgtattca gcttatactt
      601 ttacggcctt tttctcaata aggggcttga ccgcggttcg caaaaacatt attttgttaa
      661 ttatccgaat atagttttgg aggtgttcga tcgtgcgttc cgaccgcgtg caggccgctc
      721 ttcgagaatt agaccggatc ggcggagaag aaatgcttcg tggatcaaac gacaacgaaa
      781 gactcggtat tttggaaaag ctccttcccg agacttgttc ctggccgatc atgccggccc
      841 gggagctcat attccggatc cagaaagaat gggggataga tatggacgtt tgtcctttct
      901 atttcctcga agacgacgaa tccgaaacgc agtattgtct atatggaggg gaaaaggccg
      961 aatgcctctg cggccagccc tgcgaaaaat gcgttatccg cgatcaagaa gaggttgtcc
     1021 ttttttaatt tccgggaaac aagttctggc cgcgcggatc tataccaagt tcgcgcggtt
     1081 ttcttttgtg atataattat ggaaagtaat atcaatcaac atgcctgaaa aaataagaaa
     1141 aatatacagg tcgagaaccg ataggattat cttcggggtc tgtggcggac tcggagaata
     1201 tttcgaagtg gatccggtga tcttccgatt acttttcgtg ttattcgctt ttatggccag
     1261 cggaattttg atctatttga tttccgcgat cattattcct ttggagtcca aaaagaccgc
     1321 gaagacaatc gaagactaat atttttttta aaaacatttt ccgcgaaaat aaagacgctc
     1381 cggaattttc cggagcgtta tgttttgcgc ggcaaggaac gatttgctta ttcgtcgtcg
     1441 gcggcctcga tgcgatcgat gacgattctc tgggattcgt tggtaaagag attcctttct
     1501 tcgccgcgtt tggctccttc gagcgcggcg aaaagaggcg tcttgattct cgtccgtcgg
     1561 catctttcct cgtcgggagt ccaatcgccg cagaggacga tggtcctaga ttctccgtta
     1621 cgggcattat gataggtcac tctgtggcct atcgtcgcga cctcgtcgtt ttggggtttc
     1681 tcgaccagaa cggcgcatct ttgaagatca aagagatatt tcagctcctc tctcttctta
     1741 gtgatctcat cgcgaatgcg acaggcctct tcgccatcct ccttagcggt ctcgaaatcc
     1801 tcctgaagtt tagcgatggc cttttccagc ttggagattt ccgccggc
//
LOCUS       DAOPQT010000114         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_68657_length_1836_cov_2.949467, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000114 DAOPQT010000000
VERSION     DAOPQT010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68657_length_1836_cov_2.949467"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /gene="sufB"
                     /locus_tag="PKG74_02925"
     CDS             complement(<1..1170)
                     /gene="sufB"
                     /locus_tag="PKG74_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOB90253.1"
                     /translation="MPEKTFRKNIDIRKNYSEKFGFNMPEEFLLKIEKGLNEETVKRI
                     SKAKNEPAWMLEFRLKAFGFFKEKSLPSWGPDLSKIDFDDIYYYLKPVDEAKKTWDEV
                     PESIKNTFEKLGVPQAEKEFLAGVKAQYDSEVAYSSLTKELENEGIVFLSTDEGLRKY
                     PEIFEEYFGKLVPFSDNKFAALNSAVWSGGSFVYIPKGAQVKRPLQAYFRINAEKMGQ
                     FERTLIIADEGSFTQYVEGCTAPVYSTDALHAAVVEVFVKKGARFRYTTIQNWSNDVY
                     NLVTKKAKVEENAVMEWVDGNLGSKTTMKYPSCYLVGKGSRGEMLSIAYASENQHQDT
                     GAKMIHLAPDTSSKIISKSISLKGGRSSYRGRVEVSSEAKNAKCRVECDALILDED"
     gene            complement(1163..1834)
                     /gene="sufC"
                     /locus_tag="PKG74_02930"
     CDS             complement(1163..1834)
                     /gene="sufC"
                     /locus_tag="PKG74_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020214966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOB90254.1"
                     /translation="MNLSVSANSIHVLMGQNGSGKSTLAQTIMGNPFYEPVAGKIEFE
                     GVDILGLKPDERSKKGIFLSFQYPSEVAGVGIQSFLRILYNNSHEEKLTPVRFRELLF
                     AKSDMLGINREILERSLNDGFSGGEKKKMEILQMSILEPKLAILDEVDSGLDIDSLKI
                     VANAVDRLRKKNNMAVLIITHYARILKYLEPDFVHVMKDGRIAKSGNRRLAYELEERG
                     YEFDA"
BASE COUNT          405 a          491 c          390 g          550 t
ORIGIN      
        1 atcctcgtcc aggatcagcg cgtcgcattc cacccggcac ttcgcgtttt tggcttcgga
       61 agagacttcc accctacccc gataactcga tcttccgcct ttcagactta tcgacttgga
      121 aatgattttc gaagaagtat ccggagcgag atgaatcatt ttggcgccgg tgtcctggtg
      181 ctggttctcg ctcgcgtaag caatggagag catctctcct cgagaaccct tgcccacgag
      241 atagcaagat ggatatttca tggtcgtttt cgagcccagg tttccgtcca cccattccat
      301 gacggcgttt tcttcgactt tcgctttttt ggtcaccagg ttgtagacgt cgtttgacca
      361 attctggatg gtagtgtacc tgaacctagc ccctttttta acgaaaacct ccacgaccgc
      421 cgcgtgcaag gcgtcggtcg aataaaccgg agctgtgcag ccctcgacgt actgggtgaa
      481 gcttccttca tcggcaatga tcaatgttct ttcgaactgt cccatttttt ccgcgttgat
      541 ccggaaatac gcctgtaaag gccttttgac ttgggctcct ttgggaatat agacaaaaga
      601 tcctccggac caaacggcgc tgttcaaggc ggcgaatttg ttgtcggaaa acggcacaag
      661 ctttccgaaa tattcctcga agatttccgg gtattttctt aagccctcgt ccgtggacag
      721 aaaaacgatt ccctcatttt ccagttcttt tgttaaggac gaataggcga cttcggaatc
      781 gtattgcgct ttgacgccgg ccagaaactc tttctcggct tgcggcacgc ccaatttctc
      841 aaacgtgttt ttgatcgatt ccggaacttc gtcccatgtt tttttggctt cgtccaccgg
      901 cttcaaataa taataaatgt cgtcgaaatc gatcttcgac aggtccgggc cccagctcgg
      961 caagcttttc tctttaaaaa acccaaaagc tttgagcctg aactccagca tccaggcggg
     1021 ctcgtttttg gctttggaaa tccgcttcac cgtttcctcg ttcaaccctt tttctatctt
     1081 caacaaaaac tcttcgggca tgttaaaccc gaatttctcg ctgtaattct tccggatatc
     1141 gatgtttttg cggaaagtct tttcaggcat cgaattcata tcctctttct tccagttcgt
     1201 aggccaacct cctgtttccg cttttcgcga tccgaccgtc tttcatgacg tggacaaagt
     1261 cgggttccag atatttcaag attctcgcgt aatgggtgat tatcagaacc gccatgttat
     1321 ttttcttccg aagacgatca acggcattgg ccactatttt caagctatcg atatccaaac
     1381 cgctgtcgac ttcgtccaag atcgccagtt tcggctcgag aatcgacatc tggagtattt
     1441 ccattttctt tttttctccg ccggaaaatc cgtcgttcaa tgatctttcc agtatttccc
     1501 ggtttatccc aagcatatcc gattttgcaa aaagcaattc cctgaatctt acgggcgtca
     1561 atttttcttc gtgcgagtta ttgtaaagaa tccttaagaa gctttggata cccactcccg
     1621 ccacctcgct cggatactgg aacgacagga atattccttt tttggaacgc tcgtcgggtt
     1681 tcagacccaa aatatcgacg ccctcgaatt ctatttttcc ggcaacaggc tcataaaaag
     1741 gatttcccat tatcgtctgg gccagagtgc tttttccgga cccgttctgg cccatcagaa
     1801 cgtgaatact gtttgcggag acggacagat tcactt
//
LOCUS       DAOPQT010000115         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_68812_length_1833_cov_7.398200, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000115 DAOPQT010000000
VERSION     DAOPQT010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68812_length_1833_cov_7.398200"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="PKG74_02935"
     CDS             complement(<1..1531)
                     /locus_tag="PKG74_02935"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOB90255.1"
                     /translation="MVDEGPEELLHGCDAEQCFNTTMVDEGLAFYADIDFISDGFNTT
                     MVDEGYTDTITYSLTMPWFQYHHGRRERRKQRRKEPIMQLTIKNKIKTNDRGLYDFLR
                     EQCHLSKNIYNQGIFLYRMRLDAYSKLTPEEKVSLTKENKKKYYLSPSGVYKIINHEK
                     PENMKLLPAYTANLVWHAQGKEMESYFALLSKKKGTTGGDKINPPGFKPTGDKGYHTL
                     FYDLDQQTTKAVKEGLENGYFQIPVSPTYVKAHAGFEPFKIPIPKNLIGKKIKLIRIV
                     PNEKGTAFESQFIYEAGEPATPKENATGALAIDIGVHNLASCVNTNNGHSFIVDGSYI
                     DGVNKEYNRRISYLQRINSFQKKKVKASHTNQMNAITERRNNQVKEYMQLAAKKIIDY
                     VLSNNIARIIVGYSVDFKRNSNIGRNNNRKFNQIPFGVLRQKLEYLSEMNGIAYIEHE
                     ESYTSKASFLDNDPIPVYDKERKKKAKVKKGDAISVSESESVPEQKHVFSGKRVTRGK
                     YR"
BASE COUNT          403 a          414 c          411 g          605 t
ORIGIN      
        1 ctcgatattt tcctctggtt acacgcttac cggaaaagac atgcttttgc tccgggacgc
       61 tttcgctttc gcttacactt attgcatcgc ctttttttac ctttgctttc tttttgcgct
      121 ctttgtcgta gaccggtatc ggatcgttat cgagaaaaga cgctttagac gtgtacgatt
      181 cttcgtgttc gatatacgct attccgttca tctctgaaag atactccagc ttttggcgca
      241 gaaccccgaa cggtatctga ttgaacttcc ggttgttatt tcggccgata ttgctattac
      301 gtttgaagtc aacggaataa ccaacgatta ttctggcaat gttattgctt agaacatagt
      361 caatgatttt ttttgcggcc aattgcatgt attccttcac ttgattgttc ctacgctctg
      421 tgatagcgtt catttgatta gtgtgagagg cttttacctt cttcttttgg aaagaattga
      481 tgcgttggag gtaagagata cgccggttat attccttatt cacaccatcg atataacttc
      541 cgtccacgat aaaagaatgg ccgttgttgg tattcacgca tgacgcgaga ttgtgaacgc
      601 ctatatcaat tgcaagggct ccggttgcgt tttcttttgg cgttgccggt tcaccggctt
      661 cgtagatgaa ctgcgactca aatgccgtgc ctttctcgtt aggcacgata cgaatcagtt
      721 taatcttttt accaatcaga tttttaggta taggaatctt gaacggttca aatcccgcgt
      781 gtgccttaac gtaggttgga gataccggaa tctgaaagta tccgttttcg aggccttcct
      841 ttactgcctt tgttgtttgc tggtcaaggt catagaataa agtgtggtat cctttatcgc
      901 cggtaggttt gaaacccggc gggttgatct tgtccccgcc ggttgtgcct tttttcttgc
      961 taagtaaagc aaaataagac tccatctctt tgccctgcgc atgccataca agattcgcag
     1021 tatatgccgg gagaagtttc atgttttctg gtttttcgtg gtttattatt ttgtatactc
     1081 ccgaaggaga taggtagtat tttttcttgt tctctttagt aagagacacc ttctcttctg
     1141 gtgtaagttt tgaataggcg tccaacctca tacggtataa aaatatccct tgattataga
     1201 tattttttga taggtgacat tgttctcgca gaaagtcata aagcccgcga tcatttgttt
     1261 ttattttgtt tttgattgta agctgcatta taggctcctt tcgtcgttgt tttcgccttt
     1321 ctcgtcgacc atggtggtat tgaaaccaag gcatcgttag cgaatacgtg attgtatccg
     1381 tatatccctc gtcgaccatg gtggtattga aaccgtcaga gataaaatct atatccgcgt
     1441 agaaggctag tccctcgtcg accatggtgg tattgaaaca ctgctcggca tcacagccat
     1501 gcagaagctc ctcgggtccc tcgtcgacca tggtggtatt gaaacttggc gcatttgcgc
     1561 cattgcttgg cgcacggccc tccgtccctc gtcgaccatg gtggtattga aacgcgcaaa
     1621 gaatacatcg ccgcgctgaa cgcatacgag tccctcgtcg accatggtgg tattgaaacg
     1681 ctatcaaacc gtttcgcgtt gcttagcgag gattagtccc tcgtcgacca tggtggtatt
     1741 gaaaccggca acctctccac gaacggcgtt gatctctttg ttgtccctcg tcgaccatgg
     1801 tggtattgaa acccatcaaa ggctcgtata cag
//
LOCUS       DAOPQT010000116         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_68822_length_1833_cov_3.637233, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000116 DAOPQT010000000
VERSION     DAOPQT010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68822_length_1833_cov_3.637233"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PKG74_02940"
     CDS             <1..886
                     /locus_tag="PKG74_02940"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOB90256.1"
                     /translation="NAEDADPLCRGQNARSGVKKEGQTERVILTGGEPTLHPQLNEII
                     LSGKKKGFMIEVQTNGTLFGAKELEKWKKSGLDIINFAFHSHKEETSNKLRGINYGFQ
                     KIIDNIKYASQLGFELHLIHVINSVNYKDLPEWIDFVKTMGLRRFYVNLSIVVPDGWA
                     WENQWIIPKMADIGPYLRKACRKCEEYGINFDISEIVPLCHVKGFETHAVSTNFKFTG
                     LKIADDYYVGRRILDFKKPNRIVANKAPQCAECSMNEICGGFYPRLKTLYGVSDFKPR
                     HDDPRKIIDKIRPKKNNG"
     gene            879..1775
                     /locus_tag="PKG74_02945"
     CDS             879..1775
                     /locus_tag="PKG74_02945"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOB90257.1"
                     /translation="MVKRSNNRTRWVDQGHDYVSPYIYIGYDCNNNCSFCSESDEYLE
                     NLEKKDLKQLKAEIKRIRENYDFISFMGREPTLRPDFFDLLAFAKTLDFRQISVATNG
                     RLLAYPEFVKKMLASGVNQVGISFNSSDEKIHDSMTACPGSFKQTIQGIKNLTTLKKP
                     GLSILVNVPLTQKNYETLEPTLDLLIRSGVKEINILWVAPLSRRSRTKDIVCHMPTLS
                     RHVAKTLKKEKYQKSGVKFLLVEFLPCSLPKDFRDFFFPCLEKNSNKTRIDLCPECPY
                     KFSCDGVLRDYLELYGQEGLKL"
BASE COUNT          546 a          446 c          422 g          419 t
ORIGIN      
        1 caacgctgaa gatgccgatc cactgtgccg cggccagaac gcccgatccg gcgtaaaaaa
       61 agagggccag accgaacgcg taattctcac cggaggtgag ccgacgctcc atccgcagct
      121 gaatgagatc atcctctccg gaaagaaaaa aggattcatg atcgaagtcc aaacaaacgg
      181 caccttattc ggcgccaaag aactcgaaaa atggaaaaaa tccgggctcg atatcatcaa
      241 tttcgctttt cacagccata aggaggagac gagcaacaag cttcggggaa tcaattacgg
      301 attccagaag atcatcgata atatcaaata cgcgagccag ttgggatttg aactccatct
      361 gatccacgtt atcaactcgg tcaattacaa ggatcttccg gaatggatcg attttgtcaa
      421 aacaatgggt ttgagaagat tttatgtgaa tttgagcatc gtcgttcctg acggctgggc
      481 ttgggaaaat cagtggatca ttccaaaaat ggccgatatc ggcccgtatc ttcgcaaagc
      541 ctgccgcaaa tgcgaagaat acggaatcaa tttcgacatt tccgaaatcg tgcccttgtg
      601 tcacgttaaa ggtttcgaaa cccatgccgt ttcgacaaac ttcaaattca ccggattaaa
      661 aatagccgac gattattatg tcggtcggcg gatactggat tttaaaaagc cgaatcgcat
      721 agtggccaac aaagctccgc aatgcgccga gtgttcgatg aacgagatct gcggaggatt
      781 ttatccgcgt ctcaaaacgc tttacggagt ttccgatttt aagccgcgcc acgacgaccc
      841 tcgaaagatc atcgataaaa ttcgccccaa aaagaataat ggttaagcgt tcgaacaatc
      901 ggacacggtg ggtcgaccag ggacacgatt acgtaagccc gtatatttat atcggctacg
      961 actgcaacaa caactgctct ttctgcagcg aatccgacga gtatttggaa aaccttgaaa
     1021 aaaaagacct gaagcaactt aaagcggaaa tcaagaggat cagggagaat tacgatttta
     1081 taagtttcat gggtagggaa cccactttgc ggccggattt tttcgatttg ctcgccttcg
     1141 cgaaaacatt ggatttccgg caaataagcg tcgccaccaa cggccggctg ctggcttatc
     1201 cggagtttgt caaaaaaatg ctcgcctccg gcgtgaacca agtaggtatt tcttttaaca
     1261 gttcggacga gaaaatccac gattcgatga cggcttgccc aggaagcttt aagcagacga
     1321 tccagggaat caaaaacctg acgactctga aaaagccggg gttgagcatc ctcgtcaacg
     1381 tgccgctgac ccaaaagaat tacgagacgc tcgaaccgac tctagactta ttgatacgtt
     1441 cgggagtcaa agaaataaac attttgtggg tggcgccttt gagccggcgt tcccgaacaa
     1501 aggatatcgt ctgccacatg ccgacgctca gccgccacgt cgccaaaact ttgaaaaaag
     1561 aaaaatacca aaaatccgga gtcaaattcc tgctcgtcga attcctgcct tgcagcctcc
     1621 cgaaggattt ccgggacttc ttcttccctt gtctcgaaaa aaactccaac aagacgcgta
     1681 tcgatctatg cccggaatgt ccttacaagt tttcctgcga cggagtgctt cgggattatc
     1741 tcgagttata cgggcaagaa ggattgaaat tatgaacaaa tccgcggctt ccacgctcaa
     1801 agattaccaa aatttcctga aaaattatcc ggt
//
LOCUS       DAOPQT010000117         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_71293_length_1789_cov_2.842561, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000117 DAOPQT010000000
VERSION     DAOPQT010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71293_length_1789_cov_2.842561"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKG74_02950"
     CDS             complement(<1..604)
                     /locus_tag="PKG74_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOB90258.1"
                     /translation="MKRIAINGFGRIGRLFFRQAILDPEIEITAINDLGDLENLVYLL
                     KFDSVYGLFNKDVKTSGTTLSVDNKEIKFFQIKNPGELPWKDLNIDIVVESTGVFESY
                     EAARQHIAAGAKKVVLTAPAKDADTGDAKTVLVGVNEDDFQTTTLTSNGSCTTNAASP
                     VIAVLAQNPGIEKAILNTVHAYTATQGLVDGISKGSDWRRG"
     gene            complement(644..>1789)
                     /locus_tag="PKG74_02955"
     CDS             complement(644..>1789)
                     /locus_tag="PKG74_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOB90259.1"
                     /translation="FFNDASNQLIQSVMPAYFSTVLGIPPFFVGLIEGASDAISSFAR
                     VFSGWFSDKIKRRKLPSIFGYALSVATRPFFTIISGFNDAFLLRMVDRAGKGFREPPR
                     DALIVESVEKKDLGVSFGFQRAMDAAGSLIGPLLAIWFVKLYDGDYQRFFVFSFWLGL
                     FAVLTYVLVKEKRDSSESEGIVAEELDFRLIKRNKELLLFVASAFIFGLGELPIALML
                     LRPIEIGIGTLNVPLMYFLYGLAFTLAAIPFGKWSSKVGERIVIVIGFLAAIVAYSVL
                     AATASFWPTIVAFALYGLYPAAAEGARKILIAKLVEPQLLATGQGLLQTAIGVSSLFA
                     GVIGGALWTKFGAQTAFVYGATMSFTGLFVFSLVSFNNFFRKIRRPA"
BASE COUNT          470 a          469 c          453 g          397 t
ORIGIN      
        1 ggccgcgacg ccaatcgctt cctttggata tcccgtcaac caggccttgg gtagcggtgt
       61 aggcgtggac cgtgtttaaa atcgcctttt ctatgcccgg gttttgggcg agaacggcga
      121 tcaccggcga ggcggcgttc gtggtgcagc ttccgttcga agtcagggtg gttgtttgga
      181 aatcgtcttc gttcacgcca accaaaacgg ttttagcatc ccccgtgtcc gcgtctttag
      241 ccggagcggt cagaaccact tttttggctc cggccgcgat atgctgtctc gccgcttcgt
      301 aggattcgaa aactccggtc gattcgacga cgatatcgat gttcaggtct ttccaaggaa
      361 gctcccctgg attcttgatc tggaaaaact ttatttcctt gttgtcgacc gaaagagtcg
      421 ttccgcttgt tttgacgtct ttgttgaaga gtccataaac cgaatcgaac ttgaggagat
      481 aaaccaggtt ttccagatct ccaagatcgt tgatggcggt tatttctatt tccggatcca
      541 agatggcttg ccggaaaaac aaacggccga tgcggccgaa accgttgata gctattcgtt
      601 tcatgaatca attataccaa aaatcgatag gttccgcttc ttcttaggcc ggccttctta
      661 tttttctgaa aaaattgttg aaactcacca aggaaaacac gaaaagtccg gtaaaagaca
      721 tggtcgcgcc gtagacaaaa gcggtttggg ctccgaactt tgtccacaaa gcaccgccga
      781 taactccggc gaacaaagac gacactccga tggccgtctg gagaagccct tggccggtcg
      841 cgagaagctg cggctccacc agcttggcga tcaatatttt tctcgctcct tcggccgcgg
      901 ccggataaag cccgtacaaa gcaaaggcga caatggtcgg ccaaaagctc gccgtggccg
      961 caagcacgga ataagcgacg atggcggcca aaaatccgat gacgatcact atcctctccc
     1021 caaccttgct agaccatttt ccaaaaggaa tggcggccag ggtaaaagcc aagccgtaga
     1081 gaaaatacat caaaggaacg ttcaaggtcc cgatgccgat ttctatcggc cgcaaaagca
     1141 taagggcgat cggcagttct ccgagcccga aaatgaaagc gctcgccacg aaaagcaaaa
     1201 gctccttgtt tcttttgatc aatcggaaat ccaattcttc ggcaacaata ccctcggatt
     1261 cggacgaatc tcttttttct ttcacgagga cataggtcag aacggcaaat agcccgagcc
     1321 agaaggagaa aacgaaaaac ctctgataat ctccgtcgta gagcttgacg aaccagatgg
     1381 caaggagcgg cccgataagc gaaccggcgg cgtccatcgc cctctggaat ccgaaagaaa
     1441 caccgaggtc ttttttctcc accgattcga cgatcaaggc gtctcgcggc ggttcgcgga
     1501 atcctttgcc cgcccggtcg accattctca aaagaaaagc atcgttgaac ccggagatga
     1561 tcgtgaaaaa cggtcgagtg gcgaccgata gggcataccc gaaaatcgaa ggtaatttgc
     1621 gtcttttgat tttgtcggaa aaccatccgg aaaaaaccct ggcgaaactg gagatggcgt
     1681 cgctcgcgcc ctcgatcaaa ccgacaaaaa aaggcgggat acccaaaacc gtggaaaaat
     1741 aagccggcat caccgactgg atcagttggt tcgaagcgtc gttgaaaaa
//
LOCUS       DAOPQT010000118         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_72729_length_1764_cov_2.911644, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000118 DAOPQT010000000
VERSION     DAOPQT010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72729_length_1764_cov_2.911644"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PKG74_02960"
     CDS             <1..562
                     /locus_tag="PKG74_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOB90260.1"
                     /translation="FVQDKCIVSQREVKTDSLNFHQALKSLLRQDPDVVMIGEMRDSE
                     SIATAMTSAETGHLVFSTLHTNSAAQTVDRIIDSFPANQQGQITSQLAATLVGIVSQR
                     LVPRIDGGRVPACEIMFVNSAIRNLIRERKIYQIDLVIETSLQEGMTTLNRSLADLVK
                     KREISLENAELYSLNPSELRILLERM"
     gene            566..>1352
                     /locus_tag="PKG74_02965"
     CDS             566..>1352
                     /locus_tag="PKG74_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOB90261.1"
                     /translation="MRFKYSARTKEGELQAGFVEAPTRESAIGILTGHELFILTLESE
                     EKKSFLAGLPFFGERVGLRDIMVFTRQFATLLSAQVPLDKTFTTLVDQTRNTLLKRTI
                     EEVRQDIDSGLALSQALEKYENVFSNFYVNMIRSAEITGRLEEAANYLADYYERQATL
                     SGKIKNSMTYPMVTIFLFLGVAGYLIIGVLPQIEDMFVSSGIQMPGFTHALVVFGQFL
                     SRWWFMVFFAIIALVFFFADYFRSAEGKIVYDELVFRMPVFGRL"
     assembly_gap    1353..1362
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1363..>1764
                     /locus_tag="PKG74_02970"
     CDS             <1363..>1764
                     /locus_tag="PKG74_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOB90262.1"
                     /translation="AIAQFGESASILIKGGVPIAQALEISGDTIGNMIYGEALKSSAE
                     DIRRGELLSQSLAKNQDLFPPLVSQMVAVGETTGRLDELLSKLSGFYSREVDETVGNL
                     VELIQPVLMIVIGGAVAGLFASILLPMFQFVS"
BASE COUNT          379 a          466 c          468 g          441 t
ORIGIN      
        1 atttgtccag gataaatgta tcgtttccca acgtgaggtt aaaaccgaca gccttaattt
       61 ccaccaggct ctaaaatctt tgctccgtca ggatccggac gtggtgatga tcggagaaat
      121 gagggattcc gaatccatcg ccacggccat gacttcggcg gaaaccggac atttggtttt
      181 ctccaccctc cacaccaatt ccgcggccca gaccgtcgac cgcatcatcg attctttccc
      241 ggcgaaccag cagggccaga taacgtccca gctcgccgcc actttggtcg gcatcgtttc
      301 ccagaggctc gtgccgcgca tcgacggcgg ccgcgtcccg gcctgcgaaa tcatgttcgt
      361 caattccgcc atcaggaatc tgatcaggga aaggaagatc taccagatcg atctcgttat
      421 cgagacaagc ctgcaggaag gcatgaccac tttgaaccgg tcgttggccg acctcgtcaa
      481 gaaaagggaa atttctttgg aaaacgccga gctttattcc ttgaatcctt cggagttgag
      541 gattctgctc gagcggatgt aagccatgag attcaagtat tcagcgagaa ccaaagaagg
      601 agagcttcaa gccggttttg tcgaagcgcc gaccagggag tcggccatcg gcattctgac
      661 cggacacgag cttttcattc tcactttgga aagcgaggaa aagaaatctt tcctcgccgg
      721 tttgccgttt ttcggcgaac gggtaggact tcgcgacatc atggttttta cgaggcaatt
      781 cgccaccttg ctttcggccc aggtaccgct cgacaagacg tttacgacct tggtcgatca
      841 aacgaggaac acccttctca aaagaaccat tgaagaagtg cgtcaggaca tcgattccgg
      901 attggcgctt tcgcaggccc ttgaaaaata cgagaatgtt ttttcgaatt tttacgtgaa
      961 catgatcagg tccgcggaaa tcaccggccg gctggaggag gcggccaact acttggcgga
     1021 ttattacgag cgtcaggcga ctttgtccgg caaaatcaag aactcgatga cttatccgat
     1081 ggtgacgatt ttcctttttc tcggcgtcgc cggctatctt atcatcggcg ttttgccgca
     1141 gatcgaagac atgtttgtct cttcggggat ccagatgccg ggctttaccc acgccttggt
     1201 ggttttcggg caatttctgt cccgatggtg gtttatggtt ttctttgcca ttatcgccct
     1261 ggttttcttt ttcgcggatt atttccggtc ggccgaggga aaaatcgttt acgacgaatt
     1321 ggttttccgg atgccggttt tcggaagatt gtnnnnnnnn nngccattgc ccaatttggg
     1381 gaatcggcga gtatccttat taagggcggc gttccgatcg cccaagcctt ggagatcagc
     1441 ggagacacga tcggcaacat gatttacggc gaggccctga aatcctcggc cgaagatatc
     1501 agacggggag aactgctgtc gcagtctctc gccaagaacc aggatctttt ccctccgctc
     1561 gtgagccaga tggtggcggt tggagagacc accggccgtt tggacgagct tttatccaaa
     1621 ctttccggat tttacagccg cgaggtcgac gaaaccgtcg gcaacctggt ggagcttatc
     1681 cagccggtct tgatgatcgt catcggcggc gccgtggccg ggcttttcgc ctcgattctc
     1741 ctgccgatgt tccagtttgt ctcc
//
LOCUS       DAOPQT010000119         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_74893_length_1728_cov_3.821877, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000119 DAOPQT010000000
VERSION     DAOPQT010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74893_length_1728_cov_3.821877"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKG74_02975"
     CDS             complement(<1..244)
                     /locus_tag="PKG74_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOB90263.1"
                     /translation="MEKFSDQEFLEFLVKSIVDHPEDVVVSRKIDEMGVLLSLKVNPR
                     DMGQVIGRDGNTAKSIRSLLRIVGIKNNARVNLKIEE"
     gene            complement(282..545)
                     /gene="rpsP"
                     /locus_tag="PKG74_02980"
     CDS             complement(282..545)
                     /gene="rpsP"
                     /locus_tag="PKG74_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOB90264.1"
                     /translation="MLAIKLKRIGKKGQAAFRVVVAEAKSKLKGQSIEDLGYVNPHTD
                     KFELKKDRINYWIKNGARATETVKRFMAKSGMGPETSVVKKSK"
     gene            complement(662..>1728)
                     /locus_tag="PKG74_02985"
     CDS             complement(662..>1728)
                     /locus_tag="PKG74_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOB90265.1"
                     /translation="KKIVSLIEDMENAAEAQPEVQVVDGKLEEFNRLMDELDEEEKKY
                     QIELDSFNKVFRESLRQVEAKKDEIEELDDKKQQLIFQKDKALFQKSELENQLRSISR
                     KFEDFVGVSADMRMDERTAETRMMRLRAELSSMGDVDEAIVKEAEATEERHEFLSNQI
                     VDLEKASADLKTLLKDLDFKIRNEFNVALKKINDELQSFVKLMFGGGRAELKLEKIRE
                     TTREAIEAAGVLGEVIPNQEEAEEIRPGIEIDVVLPKKKIKGLEVLSGGERSLLSIAI
                     LFALISISPPPFLVLDEVDAALDERNARRFGELLKNYAKETQFVIVTHNRATMEAADV
                     LYGVTMGDDGVSRVVSLKLS"
BASE COUNT          361 a          458 c          390 g          519 t
ORIGIN      
        1 gctcttcgat cttcaaattg acgcgggcgt tgttcttgat gcccacgatc cgaagcaacg
       61 atctaatgga tttggccgtg ttgccgtcgc ggccaataac ttggcccata tcgcgcggat
      121 tgacctttaa ggacagcaaa actcccattt cgtcgatttt cctcgagacg acgacgtctt
      181 ccggatgatc gactatggat ttaactagga attccaaaaa ctcctggtcg gaaaactttt
      241 ccatgtctgc tatatctgct tgttttttat ttttttaaag attatttcga cttttttacg
      301 actgatgtct ctggtcccat cccagatttg gccatgaatc tcttaacggt ctccgtggct
      361 ctggcgccgt tttttatcca ataattgatt ctatctttct tcagttcgaa cttgtcagtg
      421 tgcggattga cataaccgag gtcttcgatc gactgtcctt tcagcttcga cttggcttcg
      481 gccacgacca cgcgaaaagc ggcctggccc ttctttccga tccttttcaa cttgatggct
      541 aacatgatct tgtaaagaca atattacccg aaaatctcaa tgcagtcaat cgggaataac
      601 tctcgttctc cgagggaagg gttctgttat tcattataac atatcgatcg atcaaaaccg
      661 atcaagagag cttcagagaa acgactcgcg aaacgccgtc atctcccatg gtcacgccgt
      721 aaagtacatc cgccgcttcc atcgtcgccc gattgtgcgt aacgatcacg aactgtgttt
      781 ccttggcgta attcttgaga agctcgccga atcggcgggc attcctctca tcgagcgccg
      841 cgtccacttc gtccaggacg agaaacggcg gaggcgaaat ggaaataaga gcgaaaagaa
      901 tggcgatcga aagaagactt ctttctccgc cggaaaggac ttcgaggcct tttatttttt
      961 tcttcggcag aacgacgtcg atttctatgc ccggccggat ctcttcggct tcttcctgat
     1021 tcggaatcac ttcgccgaga actccggcgg cttcaatcgc ctctcttgtc gtttcccgga
     1081 ttttttccag ctttaattcg gcgcggccgc cgccgaacat aagcttgacg aaagactgca
     1141 gttcatcgtt aatcttctta agggcgacgt tgaattcgtt tctgatctta aaatcaaggt
     1201 ctttgagcag ggtttttaga tcggccgagg ctttttcgag atcaacgatc tggttgctca
     1261 aaaattcgtg tctttcctcg gtggcttcgg cttccttgac gatcgcttcg tcgacatctc
     1321 ccatactaga aagctccgcc cgcagccgca tcatccgggt ttcggccgtc cgctcgtcca
     1381 tgcgcatatc ggccgagacc ccgacaaagt cttcgaactt ccgggagatg gatcggagct
     1441 ggttttccaa ttcggatttc tggaaaagcg ccttgtcttt ctggaaaatc agctgctgtt
     1501 ttttgtcgtc gagttcctcg atttcgtctt ttttggcctc gacctgccgc aggctttccc
     1561 ggaaaacctt attgaaacta tcgagctcga tctgatactt tttctcttct tcgtcgagtt
     1621 cgtccatcag ccggttgaat tcctcgagtt ttccgtcgac gacttggacc tcgggttggg
     1681 cttcggccgc gttttccatg tcttcgataa ggctcactat ttttttca
//
LOCUS       DAOPQT010000120         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_76848_length_1697_cov_2.694884, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000120 DAOPQT010000000
VERSION     DAOPQT010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76848_length_1697_cov_2.694884"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..578
                     /locus_tag="PKG74_02990"
     CDS             135..578
                     /locus_tag="PKG74_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOB90266.1"
                     /translation="MSDIDQKPKVGVGVMVFKDGKVLLGKRNDDAEKASSELHGEGTW
                     TMPGGKLDFGETLKEAACREAIEETGIKLNKLELISVTDEILTDKHFVTVGFLCEDFD
                     GDPRVMEPEEITEWRWFDLNDLPEKVFPPSAKIINNFLEKKIYRS"
     gene            complement(615..980)
                     /locus_tag="PKG74_02995"
     CDS             complement(615..980)
                     /locus_tag="PKG74_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90267.1"
                     /translation="MSLPYEKPQPIERLYQTLYDEGALERSPQGLTRNQMIEEISGYL
                     VKTESCSALHFITYCALGDEELQSVAQVYERHISPRPKEYNGMTDDDKLVALRLFAKE
                     EKLTLRIEELCKIQLRTED"
     gene            complement(1046..>1697)
                     /locus_tag="PKG74_03000"
     CDS             complement(1046..>1697)
                     /locus_tag="PKG74_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90268.1"
                     /translation="ERMYEVLLQRIEASVKAMTRRAIEENSQALEKEYNRDVEDFSQS
                     LEKPFWRIDYEDALNLLRKNGFPGLQWGNDLKADHEATVVKIMNAKENEPRPVFIMRY
                     PKEIKFFNMKVSEKDPRVVLSADLIFPFAGEGVGSAVREPNGEKLEKRLLESNMFRLH
                     KERGGTLDDFGWYLDLVKSKKTKPHAGYGLGNERLIQYILGSNDIRNCSTFRMITN"
BASE COUNT          418 a          386 c          409 g          484 t
ORIGIN      
        1 gcgaaaaatt aaacgaaggt cagattcagc aaatttcaaa cgatatgctt ttggtctatc
       61 aaaaacgcga cgatattctc gttttgttat tagtagatat tggctctcac gcttctttgt
      121 ttaataaata atttatgtct gacatcgatc aaaaaccaaa agtaggagtg ggagtaatgg
      181 tttttaaaga cgggaaagtt ttacttggta aaagaaatga tgacgcggaa aaagcgtcga
      241 gcgaactcca tggcgaaggc acctggacca tgcccggagg aaaattggat ttcggagaaa
      301 cgctgaaaga agccgcttgc cgcgaagcca tagaagaaac agggattaaa ctcaataaac
      361 tggagctgat cagcgtcacc gacgagattc tgaccgataa acatttcgta accgtcggct
      421 tcttgtgcga ggattttgac ggagatccgc gagtaatgga gccggaagaa atcaccgaat
      481 ggcgatggtt tgatctgaac gatctaccgg aaaaagtctt tccgccgagc gccaaaatca
      541 tcaataattt tctcgagaaa aagatttatc gatcgtaaga aaagccgcct ttatggggcg
      601 gcgggtcgaa ggtttcaatc ctcggttctc aactgaatct tgcacagctc ttcgatgcga
      661 agcgtcagtt tctcttcttt agcgaacagt ctgagcgcga cgagcttgtc gtcatcggtc
      721 atgccgttat attccttggg cctcgggctg atgtgccgtt cgtagacttg agctaccgac
      781 tggagctctt cgtctccgag cgcgcaatag gtgatgaaat gaagcgcgct gcagctctcg
      841 gtcttaacga gataacccga tatttcctcg atcatctggt ttctagtcaa gccttgcgga
      901 gatcgctcga gagccccttc gtcgtaaagc gtctgataaa gacgttctat cggttggggc
      961 ttttcatacg gcaaagacat attaattctc ctttcaaggt cctttgtagt cggattctat
     1021 tatggttgtt tttctaagtc aatgctcaat tcgtgatcat ccggaaagtg gaacaattcc
     1081 tgatgtcgtt tgatcccaag atatattgga tcaggcgttc gtttcccaag ccgtagccgg
     1141 catggggttt ggtctttttg gatttgacga gatcgagata ccagccgaaa tcgtccaagg
     1201 ttccgccgcg ttccttgtga agccgaaaca tatttgattc gagcaagcgt ttttcgagct
     1261 tttctccgtt tggttcgcgg acggccgacc ctaccccttc tccggcgaaa ggaaagatca
     1321 aatcggccga aagaaccact cgcggatctt tttccgatac cttcatgttg aagaacttta
     1381 tttctttcgg atagcgcatg atgaataccg gccgcggctc gttctctttt gcgttcataa
     1441 ttttgacgac cgtcgcttca tgatcggcct tcagatcgtt gccccattgg agtccgggaa
     1501 atccgttttt ccgcaagaga ttcaaagcgt cttcgtagtc gattctccaa aacggttttt
     1561 cgaggctttg ggaaaaatct tcaacatccc ggttgtattc tttctcgagc gcttggctgt
     1621 tctcttcgat cgctcttctt gtcatggctt tgaccgacgc ttcgatgcgt tgaagtagaa
     1681 cctcgtacat tctttct
//
LOCUS       DAOPQT010000121         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_78194_length_1676_cov_3.181370, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000121 DAOPQT010000000
VERSION     DAOPQT010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78194_length_1676_cov_3.181370"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKG74_03005"
     CDS             <1..185
                     /locus_tag="PKG74_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90269.1"
                     /translation="TMEVDGWENDREKIILITSADKEESNYANAIVPIVEAFVKDHDP
                     EEIPLGLINSKVYRLR"
     gene            193..1629
                     /locus_tag="PKG74_03010"
     CDS             193..1629
                     /locus_tag="PKG74_03010"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOB90270.1"
                     /translation="MEIWIINPPFKEKTTREGRCAQKKNMWSSLWPPVSLAYIAAILR
                     EDGHEVKAFDCIASETDKDIFFRESNGSPRIAVVNTSTPTIESDLEFARDFKKKFPEA
                     TVIVFGTHPSALPNECLDSGADIAVIGEPENSIRIISRSIVSGSEEELDRASFAKKNP
                     DGSISIFSGNAFSDCLDKMPFPAWDLFDLSKYLMPGKDLAPRKFLTITPSRGCPHNCS
                     FCNAGSYYGKKPRFRDPDRVVDEIERNIREFGIKDFLIWTESFTMNPEFAEKICDGII
                     NRGLKVNWVCNSRTDAASLPLFEKMKQAGCWMISFGVESGEQKILDLAKKSASVIDSE
                     KAINLAKKAGLLTVSHVIIGLPGETRESVKKTWELLDRTKPDFAQFYCAVPFPGSRLY
                     EEARRNNWIKQGSEFVDFEQMKSVMDLPTMKAEETMRLKNQSYLRYYLNVPRVFKVIR
                     RASAKGIISAGKSAVALVFSEIKGQFKP"
BASE COUNT          497 a          368 c          431 g          380 t
ORIGIN      
        1 aaaccatgga agtggacgga tgggaaaacg atagagagaa aataatattg attacgtcgg
       61 cagataaaga agagagtaat tacgcgaacg ccattgttcc gatagtggaa gcgtttgtca
      121 aagaccacga ccccgaagaa ataccgctcg gtttgataaa tagtaaagta tatcgattga
      181 gataattgaa aaatggaaat ctggatcatc aatccgccgt ttaaggagaa aacgacaaga
      241 gaaggaagat gcgcgcagaa aaagaatatg tggtcttcct tgtggcctcc ggtcagcctg
      301 gcttatatcg ccgcgattct tcgcgaagac ggacacgaag tcaaagcttt cgattgtatt
      361 gcttcggaaa cggataagga tatttttttc cgcgaatcga acggatcgcc gcgaatcgcc
      421 gtcgtcaaca cttcgacgcc gaccatagaa agcgatctgg aattcgcgag ggacttcaag
      481 aaaaagtttc cggaagcgac ggtgattgtt ttcggaaccc atccttcggc tttgccgaat
      541 gaatgccttg attcgggagc ggatatcgcc gtgatagggg agccggaaaa ctcgataaga
      601 atcatcagtc gatcgatagt aagcggatcg gaagaagaac tcgatcgagc gagtttcgcg
      661 aaaaagaacc ccgatggtag tatttccatc ttttccggga acgcgttttc cgattgcctc
      721 gataaaatgc ctttcccggc ttgggatctt ttcgatcttt ctaaatactt gatgccggga
      781 aaagatttgg ctccgcggaa gttcctgaca attacgccct ctcgaggctg cccccacaac
      841 tgttctttct gcaatgccgg atcttattac ggaaaaaagc cgaggttccg cgatcccgat
      901 cgcgtagtcg acgaaatcga aagaaacatc cgcgaattcg gaataaagga ttttcttatt
      961 tggaccgaat ctttcaccat gaacccggag ttcgcggaaa agatctgcga cgggatcatc
     1021 aataggggat tgaaggtgaa ttgggtttgc aacagccgga ccgatgccgc ctctcttcct
     1081 cttttcgaga aaatgaaaca agccggctgc tggatgatca gtttcggcgt cgagtccgga
     1141 gagcaaaaaa tactcgattt ggcgaagaaa agcgcctcgg ttatcgattc ggaaaaagcg
     1201 atcaacctcg ccaaaaaagc ggggcttctg accgtcagcc acgtgattat cggcctaccc
     1261 ggagaaacga gagaatcggt caaaaaaacc tgggaattgt tggatcgcac caaaccggat
     1321 ttcgcgcagt tttactgtgc cgttcctttt cccggatcaa ggctttatga agaggccagg
     1381 cgaaacaatt ggataaagca ggggagcgaa ttcgtggatt tcgaacagat gaaatcggtg
     1441 atggatctgc cgaccatgaa agcggaagaa acgatgaggc taaaaaacca atcgtactta
     1501 aggtattatt tgaatgtccc gagagtgttt aaagtgataa gacgggcaag cgcgaagggg
     1561 ataatttccg ccgggaagag tgccgtcgcc cttgttttct ccgaaatcaa aggacaattc
     1621 aaaccatgat caccatcatc atacccgtat tcaacgaaaa agagaccatc gagaac
//
LOCUS       DAOPQT010000122         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_80689_length_1639_cov_3.597222, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000122 DAOPQT010000000
VERSION     DAOPQT010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80689_length_1639_cov_3.597222"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PKG74_03015"
     CDS             complement(<1..794)
                     /locus_tag="PKG74_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOB90271.1"
                     /translation="MSSVKPVKLNPDSPNFFRFKKMGKDYLITSDAGNHVFLSPEDFS
                     KFVRGKIKEESALYAELKEKGFFKNDLDLTKEIAQYQKRSASLFQGPSLHIVVTTLRC
                     NYKCVYCQASSRSMTAKGYDMNLKTAKRAVDMIFESPSHVITIEFQGGEPLMNWPAVK
                     YIVEYSREKNKKIRKILLISLVTNLSVMDDEKFDFLIDNRVSICTSLDGPEKVHNINR
                     PFPGENSHKLTSDWIKKIKKIEKEKKESGKDIYHLSALVTVSKESL"
     gene            complement(795..1022)
                     /locus_tag="PKG74_03020"
     CDS             complement(795..1022)
                     /locus_tag="PKG74_03020"
                     /inference="COORDINATES: protein motif:HMM:NF038315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HxsD-like protein"
                     /protein_id="HOB90272.1"
                     /translation="MASKSTGISVRFNKKFYNLESVQKSAKQFRSLADLKISDSKSGI
                     DVRVVAKKPFSDSLIADELKNYALAEMKDII"
     gene            complement(1026..1580)
                     /gene="hxsD"
                     /locus_tag="PKG74_03025"
     CDS             complement(1026..1580)
                     /gene="hxsD"
                     /locus_tag="PKG74_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system protein HxsD"
                     /protein_id="HOB90273.1"
                     /translation="MAKKKTAAEKSKTDNKEIKIAVNPKIYPLEAIYGAAYVFLDRAY
                     VFLDGNPKTKIMVSIKSKDEENFGKADKIKSEFLNELVNCSLRRQIAERNAKLREYVA
                     GAALLGASGDINVFPVTETDNISPDELPIGTEIEETVIETETWKEDPFQIAVPWEEKY
                     AVAEKVEIAPAVKTETKKKKTKKK"
BASE COUNT          359 a          384 c          369 g          527 t
ORIGIN      
        1 ttcaaagatt ccttggaaac cgtcaccaaa gcggataagt ggtaaatgtc cttaccggac
       61 tcttttttct ctttttctat ttttttgatt ttcttgatcc aatcggaagt cagtttgtgg
      121 ctgttttctc cggggaacgg ccgattgatg ttgtggactt tttccggccc gtccaaagag
      181 gtgcagatgg aaaccctgtt atcgatcagg aaatcgaatt tctcgtcgtc catgacgctc
      241 aaattggtga ccagactgat caaaaggatc ttcctgatct ttttgttttt ctcccggctg
      301 tattcgacga tgtatttgac cgccggccag ttcatgagcg gttctcctcc ttggaattcg
      361 atggtgatga cgtggctcgg cgactcgaaa atcatgtcca ccgcccgttt agcggttttc
      421 aaattcatat cgtacccttt ggcagtcatg gaacgggaac tcgcctggca ataaacgcat
      481 ttgtaattgc atcgcaaggt ggtgacaacg atgtgcagag acgggccttg aaacaaagac
      541 gccgaccgct tttggtactg cgcgatttcc ttagtgagat ccagatcgtt tttgaaaaaa
      601 cctttttcct tcaattcggc gtacaaggca ctttcttctt ttattttccc tcggacgaac
      661 ttcgagaaat cctccggcga cagaaagaca tggtttcccg cgtcgctcgt gatcaaataa
      721 tctttgccca ttttcttgaa acggaaaaag ttcggtgagt ccggattcag ttttacgggt
      781 ttgaccgaag acatttagat gatatctttc atttccgcca gggcgtaatt tttcaattcg
      841 tcggcgatca aagaatcgct gaacggtttt ttggcgacga cgcggacgtc gatccccgac
      901 ttggagtcgc tgattttcaa atccgccaaa cttctgaatt gtttggctga tttttgcacg
      961 gattcaaggt tataaaactt tttgttgaaa cggacggaaa tccccgttga ttttgacgcc
     1021 atttgttatt ttttcttcgt ctttttcttt ttggtctcgg ttttcacggc cggagcaatc
     1081 tcaacctttt cggcaacagc gtatttttcc tcccagggca cggcgatctg gaaaggatct
     1141 tctttccagg tttcggtctc gatcaccgtc tcctcgattt ccgttccgat cggcaattcg
     1201 tccggactga tattatccgt ttccgtcacc ggaaaaacat tgatgtctcc ggaagcgccg
     1261 agaagagccg ctccggcgac gtattccctt aatttggcgt ttctttcggc gatttgccga
     1321 cggaggctac aattgacgag ttcgttcaaa aactccgatt ttatcttgtc cgcttttccg
     1381 aagttttcct cgtctttgct tttgatagac accatgattt ttgttttcgg attgccgtcg
     1441 agaaaaacat aggctctgtc caaaaaaaca taagccgcgc cgtaaatcgc ttcgagcgga
     1501 tagattttgg ggttgaccgc tattttgatt tctttgttgt cggtcttgga cttttcagcc
     1561 gcggttttct ttttagccat aacagaatta aatcggatta ctcaccttac agcagagcat
     1621 gtatccggaa gttaaagct
//
LOCUS       DAOPQT010000123         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_82554_length_1613_cov_3.060334, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000123 DAOPQT010000000
VERSION     DAOPQT010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82554_length_1613_cov_3.060334"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKG74_03030"
     CDS             complement(<1..183)
                     /locus_tag="PKG74_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90274.1"
                     /translation="MKNFWKAIKNWWATSQLAKRIKSLLWRAAMYGVAYACGWIVQNL
                     GILNLNPQITAIIGLIF"
     gene            complement(219..1190)
                     /locus_tag="PKG74_03035"
     CDS             complement(219..1190)
                     /locus_tag="PKG74_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90275.1"
                     /translation="MEDFYKKLYPDAPANPFGVDPEKIKILPDHAIADHIFAGEAGAV
                     PFNWKPFLGPRELQYWIPFCVSFSRLRCAVAKARKEGLEIDFSERDLGVESGTTKQGN
                     GLHEVSEFFRNTGIFLQKDCFFTNQMISDGWPMWDKIFDLSDVPAGAQRYKGGNHSWV
                     IGGIEAIKQQLAFSPIQIAVPVGDNWEAEIVSPPSVIRSYHAITLYFVGDYLEIQDSV
                     GKEFKKLTLDYPITGAKSFRDLPQNWKVLNKKMYKLVRDPQNQNEVYAVMPGVAKRHI
                     ATAETLRLGAQEPDRFWNWQAGDTIATATPEEWALPTAAEIHFDPVE"
BASE COUNT          359 a          423 c          416 g          415 t
ORIGIN      
        1 gaagatcaag ccgatgatgg ccgtgatctg cggattcaga ttcaagatcc cgagattttg
       61 gacgatccat ccgcaagcat aagccacgcc atacattgcg gccctccata agagggattt
      121 gatgcgcttc gccagttgcg aagtcgccca ccagtttttg atcgccttcc aaaaattttt
      181 catgttttta ttttctgctt aatgataaag atcgaccact attcgacggg gtcgaaatgg
      241 atctcggccg ccgtcggcaa ggcccattct tcgggcgtgg ccgtggcgat ggtatcgccg
      301 gcttgccaat tccagaagcg atcgggttct tgcgccccga gtctcaaagt ctcggccgtg
      361 gcgatatgcc gcttcgccac gccgggcatc acggcataga cttcattttg attttgcgga
      421 tcccggacca gtttatacat ttttttattg aggaccttcc aattctgggg caaatcccgg
      481 aaggatttgg cgccggtgat cggatagtcc agcgttaatt ttttgaattc ttttccgact
      541 gaatcttgga tctccaaata gtcgccgaca aaataaagag tgatcgcgtg atatgaccgg
      601 atcacgctcg gcggcgagac gatctcggcc tcccaattgt ctccgaccgg gaccgcgatc
      661 tgaatcggcg agaaggcgag ttgctgtttg atcgcttcga tgccgccgat gacccatgaa
      721 tgattccctc ccttgtatct ttgcgcgccg gccggcacat cggaaagatc gaagatctta
      781 tcccacatcg gccatccgtc cgagatcatc tgattcgtaa agaagcagtc tttttgaaga
      841 aagatcccgg tattgcggaa aaattccgag acttcgtgga ggccgttgcc ctgtttcgtg
      901 gtcccggatt cgactccgag atctcgttca gaaaaatcga tttcgagtcc ttccttgcgc
      961 gctttggcga ccgcgcatct caatcttgaa aaagaaacgc aaaaggggat ccagtattga
     1021 agttcgcgcg gaccgagaaa cggcttccag ttaaaaggga ccgctccggc ttcgccggca
     1081 aagatatgat cggcgatcgc gtgatccggg agaatcttga tcttctccgg atcgacgccg
     1141 aacggatttg ccggcgcatc cggatataat tttttataaa aatcttccat gatttttttc
     1201 ttgttaaaag taaggggcga ccatcggccg ccccgggagg cgactaaaga gccgccttga
     1261 ttcgctcgac tctttcgagg agagcgataa tttccggaat ggtccgattt ccaaaaagga
     1321 tatgccaaag atcatgcttg tcgcgacgga tgagaagaag attcttccga tcggcttttc
     1381 cgccgcgcga cttcgggatc aagtgatgat gagacatccg ccgggctttc cggtatctgc
     1441 ggccgcttcg gctcatgaaa cacctccaaa gaacgattta ttttttcttc ccgaggacga
     1501 tcacgcggcc gccttttttg gccgcgatct tgagcgcctc ttttgatttc aaaaagatcg
     1561 tcgacatggc cgaggccaag acttcgatct cctcgtcggg tccggatttt tcg
//
LOCUS       DAOPQT010000124         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_83887_length_1595_cov_2.755844, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000124 DAOPQT010000000
VERSION     DAOPQT010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83887_length_1595_cov_2.755844"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..>1595
                     /locus_tag="PKG74_03040"
     CDS             235..>1595
                     /locus_tag="PKG74_03040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOB90276.1"
                     /translation="MTSFEVAYKRLNLAQKQAVDAIEGPVMVIAGPGTGKTQILTLRI
                     ANILRRTDTEPENILALTFTEAGVASMRKRLIEFIGSPAYGVSINTFHGFCNDIIKNY
                     PEEFPMIIGSENINEVDQIRIVEDLIEKLPLKELKPFGDVFYYLRPILRALNDLKREG
                     VSVEKFKEAVEAEAVDFEKIDDLIYEKGPHKGKMKGKYQDLLRRINKNKDLAFFYEKY
                     ESRLREEKLYDYSDMIMEVLRTLERNNDLLLRLQEEYQYVLVDEHQDTNNSQNKILEM
                     LCNFHKNPNIFVVGDEKQAIFRFQGASIENFLYFKSLYPEAVLIALEDNYRSTKSILE
                     SAESLIHGEKPLKAKVDYPEKKISLLEFSKPETENRFVAEEIKKKIDVHVPANEIVVL
                     YRENKDAFPVAEALDKVGVPYIIESDQDVLSDLNIQKIVLLLQAIDRFGDQGKFLEAM
                     HID"
BASE COUNT          462 a          357 c          391 g          385 t
ORIGIN      
        1 attggcggtc tccatcccat tcttttgatg tcaggaacgc gcgtggatat cgcatggagt
       61 agcaaaaagt ggaaaaggca ttatggtgac agaaagagaa tgatggatgt ggcggcaata
      121 tacggtattc ctccttttgc gtcttgactc gtatgaaggg gttgttcgcg agattatcgt
      181 ggatgaccct tttttaactt tttctacgtt ggtttttggg gtatattgga aataatgacg
      241 agcttcgaag tcgcctacaa acgccttaat ttggcccaaa aacaggccgt ggacgcgatc
      301 gagggcccgg ttatggtaat agccggtcca ggcacgggaa agacccaaat tttgactttg
      361 agaatagcca atattttgcg acggaccgac accgaaccgg agaatatcct cgccctcact
      421 ttcaccgaag ccggcgttgc ttcgatgcgg aaacggctga tcgaattcat cggttctccg
      481 gcctacgggg tttccatcaa cactttccac ggattctgca acgacatcat taagaattat
      541 ccggaggagt tcccgatgat catcggttcg gaaaacataa acgaagtcga tcaaataagg
      601 atcgtcgaag acctgatcga gaaactgcct ttgaaagaac tgaagccttt cggagatgtt
      661 ttctattatc tccggccgat tttgagggct ttgaacgatt tgaagcgcga aggggtatcc
      721 gtggagaaat tcaaggaggc ggtcgaggcc gaggctgtgg atttcgaaaa aatcgacgat
      781 ttgatctatg aaaaaggtcc gcacaaagga aagatgaaag gaaaatatca ggatcttctc
      841 cggcggataa acaagaacaa agatttagcc tttttttacg aaaagtacga atcccggctc
      901 cgtgaggaaa agctttacga ttacagcgac atgatcatgg aagttctgcg gacgctcgag
      961 cgaaacaacg atctgctgct tcggcttcag gaagaatacc aatatgtcct ggtcgacgag
     1021 catcaggata ccaataacag ccagaacaaa attttggaaa tgctctgcaa tttccataag
     1081 aacccgaata ttttcgtggt cggagacgaa aagcaagcca ttttccggtt ccagggagcg
     1141 agcatcgaaa actttcttta tttcaaatct ttatatcccg aagccgtgct cattgccctc
     1201 gaggataatt accgttcgac gaagtcgatc ttggaaagcg ccgaaagcct gatccacgga
     1261 gaaaaacccc tgaaagccaa agtcgattat ccggaaaaga aaatcagcct cctcgagttt
     1321 tccaaaccgg aaacggaaaa ccggttcgtg gccgaagaaa ttaagaaaaa gatcgacgtc
     1381 catgttccgg cgaacgaaat cgttgttctt taccgggaaa acaaagacgc ttttccggta
     1441 gccgaagcct tggacaaagt gggcgttccc tacatcatcg aatccgatca ggatgtttta
     1501 tccgatctaa atatccaaaa aatcgtctta ctgctccagg cgatcgatcg cttcggcgac
     1561 caggggaaat tcctcgaagc catgcacatc gattt
//
LOCUS       DAOPQT010000125         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_87631_length_1545_cov_3.077181, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000125 DAOPQT010000000
VERSION     DAOPQT010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87631_length_1545_cov_3.077181"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PKG74_03045"
     CDS             <1..952
                     /locus_tag="PKG74_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOB90277.1"
                     /translation="DSRNQDIPYFLSNDLKTAETYVSSLQSELSNLASKKSAFRDAVL
                     SVAEKKDDLEELKNKPDALTLSTAKISLSQRENSLTDAKEKLNDHYIRAPFDGTVAKM
                     NIKNSDSVSSDSVSSGSSIATIVSEKSMAEISLNETDISRVKIGQKAVLDFDSTDDVV
                     IAGTIIEVDAVGSSSQGVVSYGAKIAFESEDERIKSGMSVNAEIKVEEKTNVLVVPAA
                     AVKSQGGRTFVSIASNPPKTIGNVAVGLSSLPTMVSVETGLENDDYVEIVSGLSEGDV
                     VVVRTVAGDSGQATITNIRSSTGGNMMMFQSGGMAAPRMR"
     gene            949..>1545
                     /locus_tag="PKG74_03050"
     CDS             949..>1545
                     /locus_tag="PKG74_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005898026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOB90278.1"
                     /translation="MIEVRNIKKTYRNGGIPSEVLKGLSFKIDDGEFVAIMGPSGSGK
                     STIMHILGCLDSPTEGRYLLDGKDVSGLSDDELAEIRKKKIGFVFQSFNLLPRCSAIR
                     NVSLPLIYSETDKEQRRARAEKCLEAVGFSKEHFDYLSNQLSGGQMQRVAIARALVNN
                     PSIILADEPTGNLDSATGKIVMETFRRLNTEEKRTIVVI"
BASE COUNT          411 a          420 c          389 g          325 t
ORIGIN      
        1 cgacagccgg aaccaggata tcccctattt tctctccaac gacctaaaaa ccgccgaaac
       61 ctacgtttca agcttgcaga gcgaactttc caacctggct tcgaaaaaga gcgctttccg
      121 ggacgcggtc cttagcgtgg cggaaaagaa agacgatctc gaggaactca agaacaagcc
      181 tgatgcgctc actctttcca ccgctaagat ttccctttcc cagagggaga acagtctgac
      241 cgacgccaaa gaaaagctga acgaccacta tatccgcgct cctttcgacg gaaccgtcgc
      301 caagatgaac atcaagaaca gcgattcggt ttcttcggac tcggtttcct cgggctcttc
      361 catcgccacc attgtctcgg aaaagagcat ggccgagata tcgcttaacg aaaccgacat
      421 ctccagagtc aaaatcgggc aaaaggccgt tcttgatttc gactcgactg acgacgttgt
      481 catcgccgga acgatcatcg aagtcgacgc cgtgggttcg tcttcgcaag gagtggtttc
      541 ctacggcgcg aaaatcgctt tcgagagcga agacgaacgc atcaaatccg gcatgagcgt
      601 caacgccgaa atcaaagtcg aggaaaaaac gaatgttttg gtcgtgccgg cggccgccgt
      661 caagtcgcaa ggcggaagaa cttttgtttc gattgcttcc aatcccccga aaacgatcgg
      721 caacgtggcc gtcggactat cttctttacc gaccatggtt tccgtggaaa ccggattgga
      781 gaacgacgac tacgtggaaa tcgtctccgg tcttagcgaa ggcgacgtcg tggtcgtaag
      841 aaccgttgcc ggcgattccg gacaagcgac gataaccaat atcaggtctt cgaccggcgg
      901 caacatgatg atgttccaaa gcgggggtat ggccgcgccg agaatgagat gattgaagtc
      961 aggaatatca aaaaaacgta tcggaacggc ggtattcctt ccgaagtcct gaagggcctc
     1021 agtttcaaga tagacgacgg agaattcgtc gccatcatgg ggccttccgg atccggaaaa
     1081 tcgaccatca tgcatatttt aggctgtctc gacagcccga ccgaaggccg atatcttctc
     1141 gacggaaaag acgtttccgg tctttccgac gacgaactgg ccgaaatcag aaagaaaaaa
     1201 atcggctttg ttttccaatc attcaatctt ctgccgaggt gttcggcaat aaggaacgtt
     1261 tccctacccc ttatctattc cgaaacagat aaggaacaaa ggagggcgcg ggcggaaaaa
     1321 tgtctggaag cggtcggatt ttccaaggag catttcgatt atctttccaa tcagctttcc
     1381 ggcggccaaa tgcagcgcgt cgccattgcc cgggctctcg tcaacaaccc gtcgattatt
     1441 ctcgccgacg aaccgaccgg aaacctcgat tcggccaccg gaaaaatcgt catggagact
     1501 ttccgtaggc tcaacaccga agaaaaacgg accatcgtcg tcatc
//
LOCUS       DAOPQT010000126         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_87648_length_1545_cov_2.275839, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000126 DAOPQT010000000
VERSION     DAOPQT010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87648_length_1545_cov_2.275839"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKG74_03055"
     CDS             complement(<1..192)
                     /locus_tag="PKG74_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90279.1"
                     /translation="MKKEAKHKKKKKSKEPTKPDHLRGKHKPEEFRQYGLFMALPTEE
                     RKEIFGFSTDAGFSKKFKVN"
     gene            complement(213..470)
                     /locus_tag="PKG74_03060"
     CDS             complement(213..470)
                     /locus_tag="PKG74_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90280.1"
                     /translation="MMKNAIRYDKKTIEKARRLFKKAVPLREIMRRTGIKSTSTIQFR
                     FDPKYRKKHALRGMKWRKKNPERWSEINRRAVARYNQKKRG"
     gene            complement(467..1192)
                     /locus_tag="PKG74_03065"
     CDS             complement(467..1192)
                     /locus_tag="PKG74_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90281.1"
                     /translation="MDIVYILGRGSKWQNNELRFSLRSIVKNFRHGKVWIIGEFPKWL
                     RNCEHVAALDPYDNKLSNAIFKIRTACRTPEISERFVLMNDDFYFLRKIDSIQAFTLG
                     TMSKAILEHKTQAGYYFHAFRETRNLLQAAGHQEPANYEVHYPIVFEKKKFLAATDAL
                     EWETTLCLFRSIYGNTFNIGNKKRIDTKVYDISELSKLTEGDLLSSSDRVVLRPKFQK
                     FIYEKFPDPSQYEDPKSDPGKLP"
     gene            complement(1146..>1545)
                     /locus_tag="PKG74_03070"
     CDS             complement(1146..>1545)
                     /locus_tag="PKG74_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90282.1"
                     /translation="QWTPEARRYHGWLDYVRAEFLGALQKAGKINRKEWRDFFYILGP
                     KPLHTGRKKCRMRIFITWSSGAHGDPENIFGSIADALFESDKYLSGEFDFNAKPGTAG
                     RVDIDIEIGDEECGTDGYRLHPRARLKMAK"
BASE COUNT          334 a          378 c          376 g          457 t
ORIGIN      
        1 attcactttg aattttttgg agaagccggc atcagtcgaa aagccgaaga tctctttccg
       61 ctcctctgtc ggcaaggcca tgaataagcc gtattgccga aattcctccg gcttatgttt
      121 gccccggagg tggtccggtt ttgtgggttc ctttgacttt ttcttctttt tatgttttgc
      181 ctcctttttc atgctatttt tttaagaaat tgtcaaccgc gctttttttg attatagcgc
      241 gcaacggccc gtctgttgat ctcggaccat cgctccggat tcttcttcct ccacttcatt
      301 ccccggagag catgcttctt ccggtatttt ggatcgaaac ggaattggat cgtcgaagtt
      361 gatttgatcc cggtccgccg catgatctcg cgcaacggca cggctttctt gaaaagccgg
      421 cgcgcttttt cgatcgtttt tttgtcgtat ctgatagcgt ttttcatcat ggaagttttc
      481 ccggatccga cttcggatct tcgtattgag aaggatccgg gaatttttcg tatatgaatt
      541 tttgaaactt cggccgaaga acgactcgat cggatgacga aagcaaatcc ccttcagtca
      601 atttcgacaa ttccgaaatg tcataaacct tcgtatcgat ccgctttttg tttccgatgt
      661 tgaaagtgtt gccgtagatc gagcggaaaa ggcaaagcgt agtctcccac tcgagcgcat
      721 cggtcgcggc caaaaatttc tttttctcaa agacgatcgg ataatggact tcataattgg
      781 ccggctcttg atgcccggcc gcctgaagta gattccgcgt ctcccggaac gcgtgaaaat
      841 aatatccggc ttgcgtctta tgctcgagaa tcgctttcga catggtcccg agcgtgaatg
      901 cttgaatcga atcgatcttt ctcaaaaagt aaaagtcatc attcatcaaa acgaatctct
      961 ccgagatctc cggagtccgg catgctgttc ggatcttgaa aatggcgttc gagagtttat
     1021 tgtcgtaagg atcgagagcg gcgacatgct cgcaattgcg gagccatttc gggaattccc
     1081 cgatgatcca aactttccca tgcctgaaat ttttgacgat cgatcggaga gaaaagcgga
     1141 gttcgttatt ttgccatttt gagccgcgcc cgaggatgta gacgatatcc atcggttccg
     1201 cattcttcgt ctccgatttc gatatcaatg tcgacgcggc cggctgttcc gggctttgcg
     1261 ttgaaatcga attctccgga gagatacttg tcgctttcaa agagggcatc ggcgattgag
     1321 ccgaagatgt tttcgggatc tccatgcgcg cccgaggacc aagtgatgaa aattcgcatg
     1381 cggcatttct ttcggcccgt atggagaggc ttcggaccga ggatatagaa aaaatctctc
     1441 cattccttcc ggttgatctt gccggctttt tggagcgctc cgaggaattc agcccggaca
     1501 taatcgagcc atccgtgata gcgtcgggct tccggagtcc attgt
//
LOCUS       DAOPQT010000127         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_88361_length_1536_cov_2.120864, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000127 DAOPQT010000000
VERSION     DAOPQT010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88361_length_1536_cov_2.120864"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PKG74_03075"
     CDS             complement(<1..526)
                     /locus_tag="PKG74_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOB90283.1"
                     /translation="MKIYTTGSFDILHRGHFNILTQAAALGDLIVGIQSDEWIEKQKG
                     RRPILTTEERIAQIRALPFVKEVIEYGDPDQRPIYDQIRPDAIVQGDDWLYSADRSDL
                     IAYLKEKNIRLILLPRTEGISTTEIRKRVERSKRNDEKFLLDRVRMVPIEDLRLYEKF
                     DSDKVSRLMEAIGKD"
     gene            complement(523..1125)
                     /locus_tag="PKG74_03080"
     CDS             complement(523..1125)
                     /locus_tag="PKG74_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90284.1"
                     /translation="MKKVIAILAAGTTKSWDRHSVKIGGETLLERIVRQLKSRGDFDI
                     VISSNKIGDHAIEGSREIVNPLTGNDLGCFYGLKDLNPDLFIFGDVYFTDRAIDRILA
                     GEDHFFGRTSESGSKRYGEMFAVRPDADFWNFLEIAWTAYQAGKLKRLWSWDLYSMMR
                     GKNFYQFGNTGDFTEIKDETEDFDKPEDLEKWFNLYPSKR"
     gene            complement(1122..>1536)
                     /locus_tag="PKG74_03085"
     CDS             complement(1122..>1536)
                     /locus_tag="PKG74_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90285.1"
                     /translation="DDAIVCEKFMERAEEAIINAIKIQGSDVALNFYFGNRSGFRRDF
                     EEGMKKGYTIRVRPHWGVAICLPTNRIDQMIKECDQFAERQDDVRIGKFLINNGIRIY
                     FPLPSLIDHRLGSETKSLVGDIGEDRHAYKFIDSL"
BASE COUNT          354 a          406 c          351 g          425 t
ORIGIN      
        1 cgtcttttcc gatcgcctcc atcagccggg agactttgtc ggaatcgaat ttttcataaa
       61 gccggagatc ttcgatcggg accatccgga cccgatcaag aagaaatttt tcatcgtttc
      121 gttttgatct ttcaacgcgc ttccggattt cggtcgtcga gatcccttcc gtccggggaa
      181 gcaaaatcaa acggatattt ttttctttga gataggcgat cagatccgat cgatcggccg
      241 aatagagcca gtcatcgcct tgaacaatcg catccggccg gatctgatca tagatcggcc
      301 gctgatccgg atctccgtat tcgatcactt ctttcacgaa cggaagcgcc cggatctgcg
      361 cgatcctctc ctcggtcgtt aagatcggcc ggcggccctt ctgcttttcg atccattcat
      421 cgctttgaat gccgacgatc agatctccga gcgcggccgc ttgagtcaaa atgttgaaat
      481 ggccgcggtg taaaatgtcg aatgatccgg tcgtgtagat tttcatcttt ttgaaggata
      541 aagattgaac cacttttcga gatcctcggg cttatcgaaa tcctccgtct cgtctttgat
      601 ctccgtaaag tctccggtgt ttccgaattg atagaaattt tttccgcgca tcatcgaata
      661 gagatcccat gaccaaagcc gtttcaattt tccggcttga tatgccgtcc atgcgatctc
      721 gagaaaattc caaaaatcag catccggccg gaccgcaaac atttcgccgt agcgtttcga
      781 tccggattcg ctcgtgcggc cgaagaaatg atcctcgccg gcaaggatcc gatcgattgc
      841 ccggtccgtg aaatagacat cgccaaaaat aaaaagatcc ggattcagat ctttcagtcc
      901 ataaaaacag ccgagatcgt ttcccgtgag cggattcacg atctcccggg atccttcgat
      961 cgcgtgatcc ccgatcttgt ttgatgagat gacgatgtca aaatctcccc gggattttaa
     1021 ctgccggacg attctctcca aaagagtctc tcctccgatt ttgactgaat ggcggtccca
     1081 cgatttcgtc gtgccggccg ctaaaattgc gatgactttt ttcatagaga atcaatgaat
     1141 ttataagcat gccgatcttc tccgatgtct ccgacgagag atttcgtttc agatccgagc
     1201 cggtgatcga tcagcgacgg gagcggaaaa tagatccgga tgccgttatt gatcaaaaat
     1261 ttcccgatcc ggacatcgtc ttgccgttcg gcgaattgat cgcattcttt gatcatctga
     1321 tcgattcggt ttgtcggcaa acagatcgcc acgccccaat gcggccggac ccggatcgta
     1381 tatccttttt tcattccttc ttcgaagtcg cgtcggaagc cggaacgatt cccaaaatag
     1441 aaatttagag cgacatcgct tccttgaatt tttattgcgt ttatgattgc ctcctcggcg
     1501 cgttccatga atttttcgca aacaatcgcg tcgtct
//
LOCUS       DAOPQT010000128         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_89165_length_1525_cov_3.573469, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000128 DAOPQT010000000
VERSION     DAOPQT010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89165_length_1525_cov_3.573469"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKG74_03090"
     CDS             complement(<1..413)
                     /locus_tag="PKG74_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOB90286.1"
                     /translation="MNKDIDTIQSEAAEAINRAKTSEEVEAIRVRYLGRKEGLLNNVL
                     KSLKDLPLEEKRRIGPLAQQARKEIEEAIDFKLKSLVEFEIPEEDITLPGKRSETGHL
                     HPLTKTENEILRIFTSLNFSVIDGPELESEYYNFDV"
     gene            complement(419..491)
                     /locus_tag="PKG74_03095"
     tRNA            complement(419..491)
                     /locus_tag="PKG74_03095"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(456..458),aa:Met,seq:cat)
     gene            complement(568..642)
                     /locus_tag="PKG74_03100"
     tRNA            complement(568..642)
                     /locus_tag="PKG74_03100"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(605..607),aa:Asp,seq:gtc)
     gene            complement(686..>1525)
                     /locus_tag="PKG74_03105"
     CDS             complement(686..>1525)
                     /locus_tag="PKG74_03105"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOB90287.1"
                     /translation="ELSSFQLQGLGLSPDIAVVLDVFPDHLDVHRSVKEYLEAKSEIA
                     KHQKKSDVVFYNKDNKNSFLIAKKSKGKKIPVSEKKFDLFSPADLKIAGPHNFKNAVM
                     AAEIGLYLGIDPGTIKATVKKYKGLPYRLELVAKKNVGGKSIEIYNDSASTNPETTAA
                     AVKSFKEPIFLIAGGKDKNLDYGSLGATLQKSSVREAILFGENKEKIKKAIGKSKVKI
                     ILTDNLIKALAAAFEDAKKYLRFAPDEKAIIVFSPGATSFDMFKDYIGRGKKFSKLAK
                     SLD"
BASE COUNT          355 a          367 c          350 g          453 t
ORIGIN      
        1 acgtcgaaat tgtaatattc ggattcgagc tccggtccgt cgatcacgga aaaattgagc
       61 gaagtgaaaa tcctcaagat ctcgttttct gttttcgtta aaggatgaag atggccggtt
      121 tcggaacgct ttcccggaag agtgatgtct tcttcgggga tttcgaattc gactaaagat
      181 ttgagtttga aatcgatcgc ctcttcgatt tctttcctcg cctgctgggc caaaggcccg
      241 atccgccgtt tttcctcgag cggaagatcc tttaacgatt tcaatacatt attcaacaaa
      301 ccttctttcc tgcccaggta acgcaccctg atcgcctcga cttcctcgga agttttggcg
      361 cgattgatcg cttctgcggc ttcagattgg attgtgtcta tgtctttatt cataaaagtg
      421 ccggcgggtg gaatcggacc accgacctaa gcgttatgag tgcttcgctc taaccactga
      481 gctacgccgg cagcgaatat ccgcctgctt tattatatta ggctaatata accgaaatta
      541 gaaggcaaac agccttttcg taaaatgcgc agcggacggg attcgaaccc gcgatctctg
      601 ccgtgacagg gcagcgcttt aagccaacta agctaccgct gcaaacgcga ttactgacgt
      661 attttaaagc cgattttcaa ataaatcaat ccaagctttt cgcgagtttg ctgaactttt
      721 ttcctcggcc gatgtaatcc ttaaacatgt cgaaactggt cgctcccggg gaaaagacga
      781 tgatcgcttt ttcgtccgga gcgaatcgca gatatttttt ggcatcctcg aacgccgcgg
      841 ccaaggcttt tatcaagtta tcggtgagaa tgattttgac tttgcttttt ccgatggctt
      901 tttttatttt ctctttgttt tccccgaaaa gaatcgcctc tcgaaccgaa gatttttgga
      961 gcgtcgcgcc aagcgatccg taatccaaat tcttgtcttt acctccggca ataaggaaaa
     1021 tcggctcttt gaacgatttg accgccgccg cggtcgtctc cggattggtg gaggcggaat
     1081 cgttgtagat ttctatcgat ttcccgccca cattcttttt ggccaccagt tccaaacggt
     1141 aaggcaaacc tttgtatttt ttgaccgtcg ctttgatggt cccggggtcg attcccaaat
     1201 aaaggccgat ttccgcggcc atgaccgcgt ttttgaaatt gtgcggaccg gcgattttga
     1261 gatcggccgg agaaaaaaga tcgaatttct tttccgaaac cggaatcttt tttcctttgc
     1321 tttttttggc gatcaaaaaa gaatttttgt tgtctttgtt gtaaaaaaca acgtcggatt
     1381 tcttctgatg tttcgcgatt tccgatttgg cttccagata ttccttaacc gaccggtgaa
     1441 cgtctaagtg atccggaaaa acatcgagca ccacggcgat atcgggcgac aatccgagtc
     1501 cttggagctg gaagctcgaa agctc
//
LOCUS       DAOPQT010000129         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_90809_length_1506_cov_3.351482, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000129 DAOPQT010000000
VERSION     DAOPQT010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90809_length_1506_cov_3.351482"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PKG74_03110"
     CDS             complement(<1..1101)
                     /locus_tag="PKG74_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90288.1"
                     /translation="MKFDFKKIDAGKIAAVVLLIVLAVFAIGAIMKRSPEPLSVGENG
                     RRFTLPEGRQIYHISQAEEIWPKLIEAIIDPVDVHIGNTQKLSVIIDDDAEIVSVEAH
                     IETDNDVVVLPLEFVKQTESESLSPEEIGEKNIFKEFLRRFQSMPKRRKASLYEGSWI
                     VKDTHDNTYHTVFIAKDAAGRENTITLAWSDACGIPMGGNATLSSNCSISTPDGVDNG
                     NLIINPGVTLTLNSTFAFNSGKTITVNGAIALGEDGALRKTNIWMVDFDNDTYPANAT
                     QYLQDTAPTNGKRRNTLNAGGIDCNDNSASIYPGTVTTGGCSCNYSSSCDESASGTTT
                     TSTCNADGTFTGGSGSCGCSRDTDGNYCGTGYT"
     gene            complement(1111..>1506)
                     /locus_tag="PKG74_03115"
     CDS             complement(1111..>1506)
                     /locus_tag="PKG74_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90289.1"
                     /translation="KTANCPEKLISPDNLPSSAGVLKLNLAEDGKLSGEINFSFANKG
                     SDFWLFRSTTNFGEQWKNLIILLTPGFEGSVSYKKDSANNRILIVGNTDETDGGFEVS
                     YRLTAPRFDYLNWPNVPKDAEPGTGLKVK"
BASE COUNT          330 a          367 c          370 g          439 t
ORIGIN      
        1 agtatacccg gttccgcaat aattgccatc cgtgtctctc gaacatccgc aagagccgga
       61 tcctccggta aaagttccgt cggcgttgca ggtgcttgtc gttgtcgtgc cgctggccga
      121 ttcgtcgcaa ctcgatgaat aattgcaaga gcagcctccc gtggtgactg ttcccggata
      181 aatgctcgcg ctattgtcgt tgcagtcgat gccgccggca ttcaaagtat ttctgcgttt
      241 tccgttggtc ggggccgtat cttgaagata ttgggtggcg ttcgcgggat aagtgtcgtt
      301 atcaaaatcc accatccaga tattggtttt tctaagcgcc ccgtcttctc ccaaggcaat
      361 cgctccgttt acggtgattg ttttaccgga attgaaggcg aaagttgaat tcaaggtcaa
      421 ggtgacgccc ggattgataa tcaaattacc gttatccact ccgtctggcg tggaaatcga
      481 gcagttgctg ctcaaggtgg cgttcccgcc catgggaatg ccgcaagcgt cgctccaagc
      541 taaggtgatt gtgttttccc ggccggccgc gtctttagca atgaaaaccg tgtgataagt
      601 attgtcgtgg gtgtctttga cgatccatga tccttcgtac agagacgcct tgcgccgttt
      661 cggcattgat tggaatcgcc ttaagaattc tttgaatata tttttctctc cgatttcttc
      721 tggggataac gactccgatt ctgtttgctt tacgaattcc aaaggtagga ccacgacatc
      781 gttgtcggtc tcgatatggg cttcgacgga gacgatttcc gcgtcatcat cgatgattac
      841 cgaaagtttt tgcgtgtttc cgatatggac gtcgactgga tcaataatcg cttcgatgag
      901 tttgggccag atttcttcgg cctggctgat atgataaatc tgtctgcctt ccggaagagt
      961 gaatcttctt ccgttttcgc cgacgctcaa cggttccggg cttctcttca taatggcgcc
     1021 gatggcgaaa acggcgagaa cgatcaacaa aacgacagcg gcgatttttc cggcgtcgat
     1081 cttcttgaaa tcaaatttca tatttttgtt ttatttgacc tttagaccgg ttcccggttc
     1141 cgcgtctttc gggacgttcg gccaattcag atagtcgaat ctcggcgcgg tcaatcggta
     1201 ggaaacttcg aaacccccgt ctgtttcatc ggtattaccg acgatcagaa tacgattgtt
     1261 tgcgctatcc tttttataag aaacactacc ctcgaatccg ggcgtgagga gaataatcaa
     1321 gtttttccat tgttctccga aattagtcgt gctgcggaaa agccagaaat cagatccctt
     1381 gtttgcgaac gagaaattga tttctccgga caatttgccg tcttcggcca agtttaattt
     1441 caaaacgccg gccgaagacg gcaaattgtc cggagaaatc aatttctccg gacaatttgc
     1501 cgtctt
//
LOCUS       DAOPQT010000130         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_94942_length_1460_cov_2.140925, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000130 DAOPQT010000000
VERSION     DAOPQT010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94942_length_1460_cov_2.140925"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..586
                     /locus_tag="PKG74_03120"
     CDS             56..586
                     /locus_tag="PKG74_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HOB90290.1"
                     /translation="MDPIKDFQSSSENILAFLKSEMQKIRSNRPNPALVEDVKVEAYG
                     DVMTINQLATINIVPPRELIISPWDKSILSNIEKAINQNNSGLSVSADSAVIRVALPS
                     LTEERREELAKLVKSLAEENRIKMRLERDKAQKIINTFPEDQKFKQKDELQKRVDKFN
                     EEVDRLIKEKLGEIDE"
     gene            592..>1460
                     /locus_tag="PKG74_03125"
     CDS             592..>1460
                     /locus_tag="PKG74_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOB90291.1"
                     /translation="MLAIEIILIIFFVAVIVAGHEFGHFLVAKLSGMKVNEFGFGYKP
                     RLFSKKIGETEYSLNALPFGGFVEIEQGKAGEPGRGFNNASFVKRLLTLLGGIVMNFL
                     IAWAAFSVLFMVGMPQALYITNVLPNSPAESAGIKSGDQILGFSDLDAFLEAVKNHEG
                     QPLALELSRDNERIKVSVTPMSLVSAETGRIGAEVTEPGISRLGFFGSIKQGFLSCFE
                     IIGLIIRRLASFFRIRGSLSEVSGVVGVIGAIHTANKLGLPYVLQLLGMVSLNFTVVD
                     LLPIPTLDGGNIA"
BASE COUNT          387 a          366 c          358 g          349 t
ORIGIN      
        1 tttatttcat cgaacagttc aataccaaca aaaaacttca gcttcaagcc gcatcatgga
       61 cccgataaaa gatttccaat cgtcttcgga gaatatcctc gcttttctta aatccgaaat
      121 gcagaagatc agatccaatc ggccgaatcc agccttggtc gaagacgtca aagttgaagc
      181 ttacggcgac gtcatgacca taaaccagct cgccaccatc aatatcgttc ctccaaggga
      241 gcttatcatt tccccttggg ataagagtat tctctcgaat atagaaaaag ccatcaacca
      301 gaacaattcc ggattgagcg tttccgctga cagcgctgtc atcagggtgg ctcttccgag
      361 cctcaccgaa gaacggcgcg aagagctcgc caaactcgtc aaatcattgg ccgaggaaaa
      421 ccggataaaa atgcgtctcg aaagagacaa agcgcaaaag ataatcaaca cttttcccga
      481 agaccaaaaa ttcaagcaaa aggacgagtt gcagaaaaga gtcgacaagt tcaacgaaga
      541 agtcgaccgt ctaatcaaag aaaaactcgg ggagatcgac gaataacatc catgctcgcc
      601 attgaaatca tattgattat tttttttgta gccgtgattg tcgccggcca cgagttcgga
      661 cattttctgg tggcgaagct tagcggcatg aaggtcaacg aattcggttt cggatacaag
      721 ccacgtcttt tctccaaaaa aatcggcgag accgaataca gtttgaacgc cttacctttc
      781 ggcggatttg tcgaaatcga acaagggaaa gcgggcgagc cgggaagggg attcaacaac
      841 gcttctttcg tgaaaagact gcttacgctt ctcggaggaa tcgtcatgaa ctttttgatc
      901 gcctgggccg ccttttccgt tcttttcatg gtcggcatgc cgcaggctct ttacatcacg
      961 aacgttcttc cgaacagtcc ggcggaatcg gccggaatca aaagcggaga ccagattctg
     1021 ggtttttccg atctggacgc ttttctcgaa gccgtcaaaa accacgaagg ccagcctttg
     1081 gctttggaat tgagccgcga taacgagaga atcaaggtga gcgtcactcc catgtctttg
     1141 gtttcggccg agaccggccg tatcggcgcc gaagtgaccg aaccgggcat cagccggctc
     1201 gggtttttcg gaagcataaa acagggattt ttgtcgtgct tcgagatcat cggtctgatt
     1261 atccggagac tggcgagctt tttccggatc cgcggaagtc tttccgaagt gagcggagtg
     1321 gtcggagtga tcggcgccat ccatacggcg aacaaattgg gtcttcctta cgtgctccag
     1381 ctcctcggga tggtttcctt gaatttcacg gtggtcgatc ttctgcccat tccgactttg
     1441 gacggcggca acatcgcttt
//
LOCUS       DAOPQT010000131         1453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_95595_length_1453_cov_1.908441, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000131 DAOPQT010000000
VERSION     DAOPQT010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95595_length_1453_cov_1.908441"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="PKG74_03130"
     CDS             <1..1388
                     /locus_tag="PKG74_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90292.1"
                     /translation="YRPEGEDEVEIYDGDPDESGSGAIKIFGGFIVRVAQQLAQGPVI
                     TFDCDLKNFVHRLDYKLVNSTYEAMTAHDIIEDIVDNFSGPGITIENVEDDATAEIQS
                     ITFNNIAPSQAIQKIADILSKEWYVDFDGDIHFFSKFSEEAPFNLDDTGGKYIFDSLH
                     IVEDFTQIRNSILVEGGEEKSTVEETEEFVGNGTQYTFPLTRKYTGISVEVGSASQTV
                     GIANIDSFDDYDVLYDFNTRSLQFNPASPPADGEVIAVSGLYYFPIAVRFREGGSIGK
                     YGERQVLIQEKDISSRTDAVARAAAEIAAYAEKISEGSFQTYESGLRAGQKITISSDL
                     RGISKQFVIQRLSGRVVSPEKILWTAEIVSVKTYEVIDLLAQIIKGSRVEVDKDAVIQ
                     NAERVNRTILVGRTITVRAPEQVIRTLLVGRDILTYLNDPPVWVAGPYHPTSLADRHR
                     PAFADRGAVAS"
BASE COUNT          345 a          410 c          388 g          310 t
ORIGIN      
        1 aatatcggcc ggaaggcgag gatgaggtcg agatctacga cggcgatccg gacgaatcgg
       61 gatccggggc gatcaagatc ttcggcggct tcatcgtgcg cgtcgcgcaa caactcgcgc
      121 aagggccggt gatcaccttc gattgcgatc tcaaaaattt cgtccatcgg ctcgactaca
      181 aactggtcaa ttctacttat gaagcgatga cggcccacga tatcatcgag gatattgtgg
      241 acaatttttc cggaccgggc atcacgatcg aaaatgtcga agatgatgcc acggccgaga
      301 tccagtcaat cactttcaac aacatcgcgc cgtctcaagc gattcaaaag atcgccgata
      361 ttttaagcaa agaatggtat gtcgattttg acggggatat ccattttttc tcaaagtttt
      421 ccgaggaggc tcccttcaat ctcgacgaca ccggagggaa atatattttc gactcgcttc
      481 atatcgtcga ggacttcaca cagatccgga actcgattct cgtcgaggga ggcgaggaaa
      541 aatccacagt cgaggagacc gaagaattcg tcggcaacgg cacgcaatac acttttccgc
      601 ttacgcgcaa atacaccggg atctccgtcg aagtcggatc cgcatcgcaa acagtcggca
      661 tcgcgaatat cgattcgttc gacgactatg atgtcctcta tgacttcaac acccgatcgc
      721 ttcaattcaa tccggcttcc ccgccggccg atggcgaagt gatcgcggtc tccggtctct
      781 actacttccc gatcgcggtc cgcttccgcg agggaggatc gatcggaaag tacggagagc
      841 ggcaagtcct catccaagaa aaagacatct cgtcgcgcac ggatgcggtg gcacgcgcgg
      901 cggccgagat cgccgcgtat gccgagaaga tctccgaagg atcctttcag acatacgagt
      961 ccggtctccg ggccgggcaa aagatcacga tctcctcgga cctccggggc atctcaaaac
     1021 aattcgtgat ccaacgcttg agcggtcgcg tcgtgtctcc ggaaaagatc ttatggaccg
     1081 cggagatcgt ctccgtgaaa acgtatgaag tgatcgacct tctcgcgcag atcatcaagg
     1141 gatcccgcgt ggaagtcgac aaggacgccg tcattcaaaa cgccgagcgc gtgaaccgga
     1201 cgattctcgt cgggcgcacg atcacagtcc gagcgccgga gcaagtgatc cggaccctcc
     1261 tcgtcggccg ggatattctc acctacttga acgatccgcc cgtttgggtt gccggtccgt
     1321 atcatccgac ttctcttgct gatcgccatc gtccggcgtt tgcggaccgg ggagcggttg
     1381 cgtcataatt gctaaagtgg tataatttaa tcatcaagcc cacgggcctc actcgcgaat
     1441 ctatggatca gcg
//
LOCUS       DAOPQT010000132         1442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_96584_length_1442_cov_3.558039, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000132 DAOPQT010000000
VERSION     DAOPQT010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96584_length_1442_cov_3.558039"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..388)
                     /locus_tag="PKG74_03135"
     CDS             complement(233..388)
                     /locus_tag="PKG74_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90293.1"
                     /translation="MAIFKPEAKPEIKYPRVMVSVKRHKSLAAEAKKKGMSIMALAEQ
                     KFKKAGK"
     gene            complement(431..697)
                     /locus_tag="PKG74_03140"
     CDS             complement(431..697)
                     /locus_tag="PKG74_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90294.1"
                     /translation="MKIRKRGNRAIIEDPQTPPETIESRIEKVLLILIGPVLGYLATT
                     GLTWVIVWLINTIFGIHFHPNFWLLGLLVYLILLLLKDIFGGKK"
     gene            complement(660..971)
                     /locus_tag="PKG74_03145"
     CDS             complement(660..971)
                     /locus_tag="PKG74_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90295.1"
                     /translation="MKKRRILFPVLLIWHDRTIDVKGASSLLFTWAFIRKGRTLTLQD
                     IDEENFTMESLFTMDIRGSIRVARKPKEVSVDSLRKIINQQKNDGDKIENPQARQPGD
                     H"
     gene            complement(976..>1442)
                     /locus_tag="PKG74_03150"
     CDS             complement(976..>1442)
                     /locus_tag="PKG74_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90296.1"
                     /translation="RDPVECLDEFLAMMNGCEKHLTHNGFRFDIPFVIEQARRLGAGG
                     LNEEELSSFESKLYRTGVDSAVLFKASKLNLSRGIGESFKQFADRVMEIRAYGLKYNV
                     GVCCDELGIDRSNIKQHRALGDVALTLEIYRKMDLLFPVLDRQSTEEQEVPF"
BASE COUNT          339 a          374 c          332 g          397 t
ORIGIN      
        1 gatgcgctcc atgcgttcct tgacattccg gatatctgaa gcatcgataa aacggccggc
       61 gctttccgtc cttgtatcga tatctgcccg agaattgaag ttccatcggc gagccgcatt
      121 ccgggcaagc gagatcttga tgagcgattc cttgtgaata gtccattttt ttgaaactga
      181 tttcgattcc ggccggcgag atcctccatc tcatcggccg agtcgaagtc cgttattttc
      241 cggccttctt gaatttctgt tccgcgagag ccatgatcga catccccttc tttttggcct
      301 cggccgcgag cgacttgtgc cgcttgaccg aaaccatgac gcgaggatat ttaatctctg
      361 gcttcgcttc gggtttgaaa attgccatga gttttttatt atttatttat taagactgga
      421 cctcgacctt ttattttttc cctccaaaaa tatctttgag aagaagcaag atcagataaa
      481 ccagtaatcc caaaagccaa aaattcggat ggaaatggat cccgaaaatc gtgttgatga
      541 gccaaacgat gacccaagtg aggccggtcg tcgcgagata tccgagaacg ggaccaatta
      601 aaatcaataa cactttttcg atccgtgatt cgatcgtctc gggcggcgtt tgaggatctt
      661 caatgatcgc ccggttgccg cgcttgcgga ttttcaattt tgtctccatc gtttttttgt
      721 tgatttatta tttttcggag gctatcgact gaaacctcct tcggctttct cgcgacgcga
      781 atcgatcccc ggatgtccat cgtgaaaagc gattccatcg tgaaattttc ttcgtcgatg
      841 tcttgaagcg tgagcgtccg gcctttccgg atgaaggccc atgtgaacag caaagacgag
      901 gcccctttta catcgatcgt ccggtcatgc catatcagca ataccggaaa caaaattctt
      961 ctttttttca tgcgattaaa acggaacctc ttgctcctcg gttgactgac gatcgagaac
     1021 cgggaacaga agatccatct tgcggtagat ctcgagcgtt agcgcgacat cgccgagcgc
     1081 ccgatgctgt ttaatgttcg accgatcaat gccgagttcg tcgcaacaaa cgccgacatt
     1141 atacttcaag ccataggcgc ggatctccat cacgcgatcg gcgaattgct tgaacgattc
     1201 tccgatcccc cgggagagat tcaatttgct cgctttgaaa aggacggccg agtcgactcc
     1261 ggtcctgtat aacttcgact caaacgagga caattcttcc tcgtttagtc cgccggctcc
     1321 gagtctccgg gcctgttcaa tgacgaacgg aatatcgaaa cgaaatccgt tatgcgtcaa
     1381 gtgcttttcg catccgttca tcatggcaag aaattcatcg agacattcaa ccggatcccg
     1441 gc
//
LOCUS       DAOPQT010000133         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_99907_length_1409_cov_2.728951, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000133 DAOPQT010000000
VERSION     DAOPQT010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99907_length_1409_cov_2.728951"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PKG74_03155"
     CDS             <1..767
                     /locus_tag="PKG74_03155"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOB90297.1"
                     /translation="INQAIGSFNLVKPDVVISVPAGITPTERRAVINAAIEAGAKNAY
                     VVREPLLAALGAGIPINSSSGNMTVNIGGGTSEVAVISLGSIVCWSSLRIAGNKFDQA
                     IIDFIKKKYSLAIGEQTAEAAKIAVGSAVPIKNTRLEFEIRGRDLVSGLPKDVVVNSN
                     EIAEALNPLLLEIAGSVQNVFNETPPELVADIMEKGIIISGGGADLRNIDQFLTRILG
                     VESYIAEDAILCVAKGSGMILNHLETYKKTLLNKTA"
     gene            768..>1409
                     /locus_tag="PKG74_03160"
     CDS             768..>1409
                     /locus_tag="PKG74_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOB90298.1"
                     /translation="MIIGHERQQKFFDRLVKAGELSGSYIFFGESETGKFSFAESIAK
                     RLEPDSRTLTELLIVKPDEKDSIGIDAVRRLKRFLSEKPVNSGARSVIIDEAESLTNQ
                     AENALLKIAEEPPEKSLIVMIVRNPDTLLPTLVSRFRKIYFSRVKADEISAWLEKEKG
                     ISSVVAKAAAESSFGRPGLALRIAEKNFTVPEELESLADYRAFVKSEIMKLYKD"
BASE COUNT          360 a          374 c          374 g          301 t
ORIGIN      
        1 tcatcaacca ggcgatcgga tctttcaatt tggtgaaacc ggatgtcgtt atctcggtgc
       61 cggccggaat cacgccgacc gaacgccggg cggtgatcaa cgcggccatc gaagccggag
      121 ccaaaaacgc ctatgtggtg agggaacccc ttctcgccgc tctcggagcc ggaattccga
      181 ttaattcttc ttcgggaaac atgactgtca acatcggcgg aggaacaagc gaagtggcgg
      241 tcatttcttt gggaagcatt gtctgctggt ctagcctccg gatcgccgga aacaaattcg
      301 atcaggccat catcgatttc atcaaaaaga aatattcttt ggccatcgga gaacagacgg
      361 ccgaggcggc caaaatcgcg gtcggatcgg ccgttcccat caaaaacacc cgtttggagt
      421 tcgagatccg cggccgagat ctggtttccg ggcttccgaa agacgtggtc gtcaattcga
      481 acgaaatcgc cgaagctttg aatccgctcc tgcttgaaat cgccggctcg gtgcagaatg
      541 ttttcaacga aactccgccg gaattggtgg cggacatcat ggaaaaaggc attatcatct
      601 ccggcggcgg cgccgatctg cggaacatcg accagttttt gacgcggata ttgggcgtcg
      661 aatcctatat cgccgaggac gccatcttgt gcgtcgccaa gggatcggga atgatcctga
      721 atcatctcga aacctacaag aagactttac tcaacaagac cgcctagatg atcatcggcc
      781 acgaacgcca gcagaagttt ttcgacaggc tcgtgaaagc gggagaactc tcgggaagtt
      841 acatcttttt cggagaatcc gagacgggaa agttctcttt cgccgaatcg atcgccaaac
      901 gcctcgagcc cgattcccgg accttgaccg aacttcttat cgtgaagccg gacgaaaaag
      961 actcgatcgg catcgacgcc gtaagaaggt tgaaacgatt cctctcggaa aaaccggtga
     1021 actccggagc gagatcggtt ataatagatg aagcggagtc gttgaccaac caggccgaaa
     1081 acgccctctt gaaaatcgcc gaagagccgc cggaaaaatc gcttatcgtg atgatcgtga
     1141 gaaatccaga cacccttctg ccgacgcttg tttcgcgctt cagaaagatt tatttcagcc
     1201 gcgtcaaagc cgatgaaatc tcggcttggc tcgaaaaaga aaagggaatt tcttctgttg
     1261 tcgccaaggc cgcggccgaa agctctttcg gccgtccggg attggctttg cggatcgccg
     1321 agaaaaactt cacggtgccc gaggagcttg aatctttggc cgactaccgc gctttcgtca
     1381 aatccgaaat catgaaatta tacaaagac
//
LOCUS       DAOPQT010000134         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_102975_length_1379_cov_3.335347, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000134 DAOPQT010000000
VERSION     DAOPQT010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102975_length_1379_cov_3.335347"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..91
                     /locus_tag="PKG74_03165"
     CDS             <1..91
                     /locus_tag="PKG74_03165"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90299.1"
                     /translation="KSLFRDQIGQFVYNKTNRRPMILPVIIEV"
     gene            complement(148..>1379)
                     /locus_tag="PKG74_03170"
     CDS             complement(148..>1379)
                     /locus_tag="PKG74_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOB90300.1"
                     /translation="ICEAVDQTRGWFYTLLAVSALLGEKAPYRNVICLGLVLDKNGLK
                     MSKSKGNIVDPWSMIEKYGVDPIRWYFYTVNDPGEPKRFDEADLGKALRQFFFIIFNV
                     FSFYELYADKSVELKKKPKAVLDRWILARMYETEKTVSRNLDAYNIGDAGREMEKFVE
                     DLSRWYLRRSRERFQRPRSEEDFKEASAVLLECLKSLSQILAPFTPFWSEALYLSLPL
                     KKKAVSVHLTDWPRLPHSWADSKLLRLMVEAREIVAEALAERNAKAIKVRQPLQTLKF
                     RNKSLEKYPEILELVKEEVNVKEAVFDESLKTPIWLDDKITADLKAEGLVRELMRMAQ
                     DLRQKSGLKPGESIELFVDASQELTDVFSFRTKELESKLFAKKIEFKKSEAIFTGESE
                     DTLDGNPVWIGIRKISD"
BASE COUNT          324 a          360 c          332 g          363 t
ORIGIN      
        1 aaaatccctt ttccgggacc agatcggcca attcgtctac aacaagacca accgccggcc
       61 gatgatccta ccggtgatta tcgaagtctg atcaataaaa actcccctta cggggagttt
      121 tttcatcgga tggctaccgg acccgggcta gtcgcttatt tttctgatcc cgatccagac
      181 cgggtttccg tcgagcgtat cttcgctttc tcccgtgaaa atcgcttcgg atttcttgaa
      241 ttctattttt ttggcgaaga gtttagattc caattctttg gtccggaaag aaaagacatc
      301 ggtgagctct tgggaggcgt cgacgaaaag ctcaatcgat tctccgggct ttaatccgga
      361 tttctggcga aggtcctgcg ccatgcgcat gagttcgcgg accaagcctt cggctttgag
      421 gtcggccgtg attttatcgt cgagccaaat cggggttttc agactctcgt cgaaaaccgc
      481 ttctttgacg ttcacctctt ctttcaccag ctccaggatt tccgggtatt tctccagact
      541 cttgtttcgg aacttcaaag tctgcaaagg ctgtcttact ttgatggctt tggcgtttct
      601 ttcggccaga gcttcggcga cgatctctct tgcttcgacc atcagccgca agagtttgga
      661 atcggcccaa gaatgcggca agcggggcca atcggtcaaa tgaaccgaaa ccgctttttt
      721 cttcaaaggc aaggaaagat acaaagcttc gctccagaaa ggagtgaacg gagcgagaat
      781 ttgactcaaa gacttcaagc attccaaaag cacggccgag gcttccttaa agtcttcttc
      841 cgaacgcgga cgctggaaac gctcgcgcga acgcctcaaa taccaacggc tcaaatcttc
      901 gacgaatttt tccatttcgc gtccggcgtc gccgatattg taggcgtcaa gattccggga
      961 aacggttttt tctgtttcgt acatccgcgc caggatccaa cggtcgagaa ccgctttcgg
     1021 ttttttcttc aactcgacgg atttatccgc gtaaagctcg tagaaggaaa agacgttgaa
     1081 aataatgaag aaaaactgcc tcaaagcttt accgaggtcc gcctcgtcga atcgcttcgg
     1141 ctctcctgga tcgttcacgg tgtagaaata ccaacggatc ggatcgactc cgtatttctc
     1201 tatcatcgac caggggtcga cgatgtttcc tttggatttg gacattttga gtccgttttt
     1261 gtcgagcacc aaaccgagac aaatgacgtt ccgataaggc gctttttctc cgagcaaagc
     1321 ggagacggca agcagagtgt aaaaccagcc ccgagtctgg tccaccgctt cgcagatga
//
LOCUS       DAOPQT010000135         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_103631_length_1373_cov_2.569044, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000135 DAOPQT010000000
VERSION     DAOPQT010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103631_length_1373_cov_2.569044"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKG74_03175"
     CDS             <1..381
                     /locus_tag="PKG74_03175"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90301.1"
                     /translation="VESLLGKVGFTKTADLAEELIKKDLEKSLKTLSDINEAGYNLVE
                     LNHDLIHYLRRVLVLHFNPDMKNVFESELTPDELETLIKQSKEADPEKLTAMLKSLIR
                     AYSEMRYSPFAIVPLEMAIIENLK"
     gene            382..1179
                     /locus_tag="PKG74_03180"
     CDS             382..1179
                     /locus_tag="PKG74_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOB90302.1"
                     /translation="MDKASGFLLGFMPSFNSDLLEQIEFAKRHFDFLELTLPYNLRPW
                     SPEYIESVRKSLGKFSLVGHLHWDIDLTALDGEKNAKEGLQLFRKLGTSKVIIHPSFP
                     ENVGIDEALRKNMEALKALTDFCDPLNIDLLAENGDPTIPDIVEKLRYLTDQIPNLNI
                     ALDIGHAVNNWRKFAEVLQDKTKHLHLHYSDGVDDHLPFPDNIILKEIVDFWGNKNMT
                     ATLEIFRKKHGGTLEEIEGSKREKILLKQIEMARKRRFDVLILNKHF"
     gene            complement(1194..>1373)
                     /locus_tag="PKG74_03185"
     CDS             complement(1194..>1373)
                     /locus_tag="PKG74_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90303.1"
                     /translation="EGASEETVKEIASILGFDYSRAKFCSIDVLYSEKYGFSTDVINS
                     HTPRLLFDMKNPFIK"
BASE COUNT          439 a          279 c          282 g          373 t
ORIGIN      
        1 gtcgaaagct tgctcggaaa agtcggtttc accaagaccg ccgatctggc cgaggaactc
       61 attaagaaag atttggagaa atctttgaaa actctatcag acataaacga ggccggatac
      121 aacctggtgg aactgaatca cgatttgatc cattatcttc gccgcgtact cgtgcttcat
      181 ttcaatccgg atatgaaaaa cgttttcgag tctgaactga cacccgacga actcgaaact
      241 ttaataaaac aatccaaaga agccgatccg gaaaaactga ccgccatgct gaaatctctg
      301 atccgcgcct acagcgaaat gcgttactcg ccttttgcca tcgttccttt ggaaatggcg
      361 attatcgaga atttaaaata aatggacaaa gcaagcggtt tcctactcgg ttttatgccg
      421 agttttaaca gcgatctttt ggaacaaata gaattcgcga agcgacattt tgattttttg
      481 gaattgactt taccttataa tcttcggccg tggtcgcccg aatatatcga atctgtcaga
      541 aaatctttgg gaaagttttc tcttgtcggg catctccatt gggatatcga tttgacggct
      601 ttggatggag aaaagaatgc gaaggaaggc cttcaattat ttagaaaact aggaacatcc
      661 aaagtaatta ttcatccgtc tttccctgaa aatgttggca ttgatgaagc tctccgaaaa
      721 aatatggaag ccttaaaagc gctcaccgat ttctgcgatc cgttaaacat cgatcttctg
      781 gcggagaatg gagatccaac cattccggat attgtcgaaa aactccgata tcttaccgat
      841 cagattccca acttaaatat agctttggat atcggtcatg ccgtaaataa ttggagaaaa
      901 ttcgccgaag ttctccaaga taaaaccaaa catcttcatt tgcattatag cgacggtgtg
      961 gacgatcacc tgccttttcc cgacaacatc attttaaaag aaattgtgga tttttgggga
     1021 aataaaaata tgacggcgac tttggagatc tttagaaaaa aacacggcgg gactttagag
     1081 gaaatcgagg gatcaaaaag agaaaaaatt ttattaaaac aaattgaaat ggcaagaaaa
     1141 aggcgcttcg acgtcctaat attaaacaaa cacttttgag gctgaaaatc aagctacttg
     1201 ataaacggat ttttcatgtc gaataaaaga cgaggggtat ggctattgat gacatcggtc
     1261 gaaaatccgt atttttcgct gtatagaaca tcgatcgaac agaatttggc tctggaataa
     1321 tcaaaaccca aaatcgaagc gatttccttg accgtttctt ccgatgctcc ctc
//
LOCUS       DAOPQT010000136         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_103710_length_1372_cov_3.044039, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000136 DAOPQT010000000
VERSION     DAOPQT010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103710_length_1372_cov_3.044039"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PKG74_03190"
     CDS             complement(<1..1178)
                     /locus_tag="PKG74_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOB90304.1"
                     /translation="MKRVYLDFAATTPLDKAVFKIMRPYFSEYFGNPGAIHSFGQEAM
                     KAIDQSREKIIRLLGGNILEDFRNVIFTGSATEANNLALRGSVKEFYNLKRSDKYLPV
                     KIIVSNMEHDSVLKTAEDLEAEGIEVVYLPVDRKGLVDIGKLKASLDQRTALVSVHYV
                     NNETGVMEPIMEIGKIIKSFRENLGSKEIFPLFHSDCVQAFQYFDCSPKTIGADLITL
                     SGHKMFGPKGVGVLYFDRIVSGRERQAGKIIGLKPILTGGGQEFGLRSGTENVPGIVG
                     LTAAAEISDRKRKESFKKVLGLRDYFAKNLKTLDRKITLNTPLEKSSPHILNIRFQGI
                     SSEEMIGRLDLLGIAASAGSACSSRSLTLSHSLLATGLPEKETAESVRFSFSRETKKS
                     "
BASE COUNT          332 a          331 c          329 g          380 t
ORIGIN      
        1 tcggattttt tagtttcccg gctgaagctg aaacggacgc tttcggccgt ttctttttcc
       61 ggcaggcccg tggcaagaag gctgtgggat aaggtcaagc tacggctcga acaagccgag
      121 ccggccgaag cagcgatacc caacaaatcg agccgaccga tcatttcttc ggagcttatt
      181 ccttgaaaac ggatattcaa gatatggggg ctgctttttt cgagcggagt gttcaaagtg
      241 attttccggt caagggtttt caggtttttc gcgaaataat cgcggagacc caaaactttt
      301 ttgaaagatt cttttctttt tcggtcgctg atttccgcgg ctgcggtcaa ccccactatt
      361 cccggaacgt tttccgttcc cgaacggaga ccgaactctt gtccgccgcc ggtaagaatc
      421 ggcttcagac cgatgatctt tccggcctgg cgttcccttc cggaaacgat tcggtcgaaa
      481 tataaaactc cgacaccttt cggaccgaac atcttgtgtc cggataaggt gatgagatcg
      541 gcgccgatag ttttcgggga gcaatcgaaa tactggaaag cctggacgca gtccgaatgg
      601 aaaagcggaa aaatttcttt gcttcccaga ttctcgcgaa aactttttat gattttcccg
      661 atttccataa tcggttccat gacgccggtt tcgttgttga cgtagtgaac cgaaaccaaa
      721 gctgttcttt gatcgagaga ggcttttaat tttccgatgt cgaccagacc ttttctgtcg
      781 accggaagat aaacgacttc gattccttcg gcttccaagt cttcggcggt ttttaggacc
      841 gaatcgtgct ccatgttgga gacgattatt tttaccggaa gatatttgtc gcttcttttc
      901 agattgtaaa attccttgac gcttccacgc aaagccagat tgttggcttc ggtggccgaa
      961 ccggtaaaaa taacgttcct gaaatcctcc aaaatatttc ctcccaaaag ccggattatt
     1021 ttttcccggg attgatcgat cgccttcatc gcttcctgcc cgaaagaatg gatcgctccc
     1081 ggattgccga aatattcgga aaaatacggc ctcattattt tgaagaccgc tttgtcgaga
     1141 ggcgtggtcg cggcaaagtc caaataaacg cgtttcatat tatgagctta aaccgaaacc
     1201 cggaaaaaca aaatcagaaa aggctcgagg gcgcggattc gagatcgcgg atgacccggg
     1261 aaaaatcaag gctgactttg gcgatctctc cggttttttt gtccggaacg agaagatatc
     1321 cctgatcttt ggccaattcg tacaaatcct tggtcaattt gacgtcgttc tg
//
LOCUS       DAOPQT010000137         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_104426_length_1365_cov_3.863359, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000137 DAOPQT010000000
VERSION     DAOPQT010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104426_length_1365_cov_3.863359"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PKG74_03195"
     CDS             <1..732
                     /locus_tag="PKG74_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOB90305.1"
                     /translation="GAEFISEELGRKIEGVDVTALGRAHRVVSTKDNTTIVGGAGDKK
                     AIDSRISQLKAQIQKTDSDYDKEKLEERLGKLSGGVAVIKVGAPTESSQKELKQRVED
                     AVAATKAAMEEGIIPGGGMALFNVEGKISISGGAVEAAAAKVISKALQAPINAIVLNS
                     GEKPDEIVPQLKKEAEGKKWWGFNALTNEFCDLKEKGIIDPLKVTKTAFVNAVSVASN
                     YLTIGSAITDIPKKEEHNHPEMPEY"
     gene            820..1365
                     /locus_tag="PKG74_03200"
     CDS             820..1365
                     /locus_tag="PKG74_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOB90306.1"
                     /translation="MYLLLGVIAVIFLWLVGSYNSLVTLKTRVKEALSDIDVQLKRRF
                     DLIPNLVETVKGYMSHEANVLEKVTEARASVAQSKGNPLERDKAESALSGTLKTLFAV
                     SENYPELKANTNFVELQRELADTENKIQAARRFYNSNVREMNIRVQRFPTNLIAGVFG
                     FKAEPFFETEAGEKEPVKVQF"
BASE COUNT          403 a          324 c          367 g          271 t
ORIGIN      
        1 ggagcggagt ttatctccga agagctcgga agaaagatcg agggagtgga tgtgaccgct
       61 ttgggacggg cccatcgcgt ggtgtcgacc aaagacaata cgacgatcgt cggcggagcc
      121 ggagacaaga aagccatcga ttcccgtatc agccagctca aggcgcaaat ccagaaaacc
      181 gattctgatt acgacaaaga aaaactggaa gagcgtttgg gcaagctttc cggcggcgtg
      241 gcggtcatta aagtcggagc gcccacggaa tcgagccaga aagaattgaa gcaaagggtg
      301 gaagacgccg ttgccgccac caaggcggcc atggaagaag gtattattcc cggaggcggc
      361 atggcgcttt tcaatgtcga ggggaagata tccatttcgg gcggagcggt tgaggccgcg
      421 gccgccaagg tgatctccaa agcccttcaa gcgccgataa acgccattgt cctaaacagc
      481 ggagaaaagc cggacgagat cgtcccgcaa ttaaaaaaag aagccgaagg caagaaatgg
      541 tggggattca acgctttgac gaacgaattc tgcgatctca aggaaaaagg gattattgat
      601 ccactcaaag tgacgaaaac cgccttcgtg aacgccgttt ccgtggcgag caattatctc
      661 accattggtt cggccatcac cgacattccg aaaaaagaag aacacaacca tccggaaatg
      721 ccggaatact agtttgcctt ttaaccgcgc ccaaaaagcg cggttttagc cttcgtgtta
      781 taatcaaaac agtcgttaaa aacaaataaa gcaatcaata tgtatctttt attaggtgta
      841 atcgcggtaa tctttctctg gttggtcggt tcttacaaca gcctggtgac cctgaaaacc
      901 agggtcaagg aggctttgtc cgacatcgat gtccaattaa aaagacgttt tgatctgatc
      961 cccaatctgg tggaaacggt taaaggctat atgtcgcacg aagccaatgt tttggaaaag
     1021 gtaaccgagg cgagggcgag cgtcgcccaa agcaagggaa atcccttgga aagggataag
     1081 gccgaaagcg ccttgagcgg gaccttgaaa acccttttcg cggtttccga gaactatccc
     1141 gaacttaaag ccaacaccaa tttcgtggag cttcagcgcg agctcgccga caccgaaaac
     1201 aaaatccagg cggcccgtcg attctacaac agcaacgtca gggaaatgaa tatcagagtt
     1261 cagaggtttc cgaccaatct gatcgccgga gttttcggat tcaaagcgga gccgttcttc
     1321 gaaaccgagg ccggagaaaa agaaccggtt aaagtccagt tttaa
//
LOCUS       DAOPQT010000138         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_104657_length_1363_cov_2.672783, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000138 DAOPQT010000000
VERSION     DAOPQT010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104657_length_1363_cov_2.672783"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PKG74_03205"
     CDS             <1..487
                     /locus_tag="PKG74_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90307.1"
                     /translation="GFLEGDASQNIIQEVSYSQNQVVIDRLAKVLSLSRKIGVLLTLV
                     CATVAIIISFNTIILAIYSNKEEIAIMRLVGASNAFIRGPYVIVGIVYGVIAVLAAMI
                     IMAPFIYAASPYVSVFITGMNLWGFFLGSFFKFLGYNLLFGVGIGVISSVIVIRKYLE
                     I"
     gene            700..>1363
                     /locus_tag="PKG74_03210"
     CDS             700..>1363
                     /locus_tag="PKG74_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOB90308.1"
                     /translation="MNLRNIAIIAHVDHGKTTLVDALLKQSENFKIKTDNPTDLIMDS
                     NELEREKGITIFSKNASIKYKETKINIVDTPGHADFGGEVERIMRMVDGALLLVDAKE
                     GPMPQTKFVLRKAIEAGHKIIVVINKIDKSDARPNWALNATYDLFIELGATDEQIDFP
                     VIYASAVLGKAGTEPDLKTMKNIEPLFQAIIDRIPAPKADKNGPLQMLTVNLQYDNYR
                     GRI"
BASE COUNT          352 a          347 c          312 g          352 t
ORIGIN      
        1 cggttttctc gaaggcgacg cttcgcaaaa tatcatccag gaggtgagct acagccagaa
       61 ccaggtggtt atcgaccggt tggccaaagt tttgagcctt agccgcaaga taggcgtctt
      121 gcttactttg gtctgcgcca ccgtggccat catcatttcc ttcaacacca ttatcctcgc
      181 catttactcg aacaaggaag aaatcgccat catgcggctc gtcggcgctt cgaacgcctt
      241 tatccggggg ccgtatgtta ttgtcggaat cgtctacggc gtgatcgccg ttctcgcggc
      301 catgatcatc atggcgccct ttatctacgc ggcctcgcct tatgtttccg tctttatcac
      361 cggcatgaac ctttggggat ttttcctcgg atcgttcttc aaattcttgg gatacaatct
      421 acttttcggg gtcggcatcg gcgtcatctc gagcgtcatc gtcatccgga aatacctcga
      481 gatttagggt tgtggataaa tcgcgctttc cgggtttgct tttatcgaga ctctgctata
      541 ataaataaag gaatacaaaa aatcgccttg tgaggaggcg gtttttttat ggcttttcgc
      601 gtcgtccgcc ttgcaacttt ctaaaaaata ttgtattaaa agctctgata aaggccattg
      661 atcgaccaaa aaggcctttt tatttttatt atcgacatca tgaatttaag gaatatcgcc
      721 atcattgctc acgttgatca cggcaagacc actttggtcg atgccttatt gaagcaatcc
      781 gaaaatttca agatcaagac cgacaacccg accgatctta tcatggattc gaacgaactc
      841 gagcgggaaa aaggcatcac tattttttcc aaaaacgctt ccatcaaata taaggaaaca
      901 aaaatcaaca tcgtggacac gcccggacac gccgatttcg gcggggaagt ggagcgcatc
      961 atgagaatgg tcgacggagc tctacttctc gtcgatgcca aagaaggtcc gatgcctcaa
     1021 actaagtttg ttttgcggaa agcgatcgaa gccggacata aaatcattgt cgtcatcaac
     1081 aaaatcgaca agtccgacgc gagacctaat tgggctttga acgccaccta cgatcttttc
     1141 atcgagctcg gagcgaccga cgaacagatc gattttcccg tgatttacgc ttcggccgtc
     1201 ctaggcaagg ccggaaccga gccggatttg aagacgatga aaaacatcga gccacttttt
     1261 caggcgatca tcgaccgtat cccggccccg aaagccgata aaaacgggcc gcttcaaatg
     1321 cttacggtaa acctccaata cgacaattac cggggccgca ttg
//
LOCUS       DAOPQT010000139         1353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_105726_length_1353_cov_2.651002, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000139 DAOPQT010000000
VERSION     DAOPQT010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105726_length_1353_cov_2.651002"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..>1353)
                     /gene="cysS"
                     /locus_tag="PKG74_03215"
     CDS             complement(65..>1353)
                     /gene="cysS"
                     /locus_tag="PKG74_03215"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOB90309.1"
                     /translation="CGPTVYDFSHIGNFRTYIFEDLLRRTLEWNGFKVKQVMNITDVE
                     DKIIKKAIEESKTISEITLPYTRIFFSDLKDLNIEKAAVYPRATKHVKDMLRMVQALV
                     EKGFAYRGKDDSVYFEIDKFPEYGKLAGLDKVTLKSGARVESDEYDKEEARDFVLWKA
                     MKPKEPSWPSDFGPGRPGWHIECSAMSMRYLGPHFDIHTGGVDNIFPHHENEIAQSEA
                     STGKPFVNYWMHSEHLMVNGEKMSKSLDNFYTLRDIKERGFEPLAFRYATLTTHYRSK
                     MNFTWEALEASQNALNNLRFEIAKLKMFAAESGRGSLDKENKFCSAFTEAVNNDLNVP
                     EALGIAWQVVKDDSLSSKQKLGLLLDFDNIFGLGLKNVKKPVIPRKIKAIVKERELLR
                     ANQQFVQSDALRKKIEALGYKLEDTENGPIVTEKIK"
BASE COUNT          274 a          312 c          368 g          399 t
ORIGIN      
        1 aaccgattgc tcggcgtcca aatcgtgggg cggcattttt aggtctttcg aatccatact
       61 cggattattt tattttttct gtgacaatcg ggccgttttc cgtgtcttcc agcttatatc
      121 ctaacgcttc tattttcttc ctcaaagcgt cggattgaac aaactgttga ttagctctta
      181 ataactcccg ttctttgaca atggctttga ttttccgggg aataaccggt tttttgacgt
      241 ttttgagacc taggccgaag atgttgtcga aatcgagcaa aagcccgagt ttttgcttcg
      301 aagacaagga atcgtctttg accacttgcc aggcgatacc caaagcctcg ggaacgttca
      361 ggtcgttatt caccgcttcg gtaaaagccg aacaaaactt gttttcttta tccaaggaac
      421 cgcggccgga ttcggccgcg aacattttaa gcttcgcgat ttcgaagcgc agattgttca
      481 gggcgttttg gctcgcttcg agcgcttccc aggtgaaatt cattttggag cggtaatggg
      541 tagtgagcgt cgcgtagcgg aaggcgagcg gttcgaatcc gcgctctttg atatcccgca
      601 gggtgtaaaa attgtcgagc gacttcgaca tcttttctcc gtttaccatc aagtgttcgg
      661 agtgcatcca gtaattgacg aacggctttc cggtcgaggc ttcggattgg gcgatttcat
      721 tttcgtggtg gggaaagatg ttgtccacgc ctccggtatg gatatcgaag tggggtccga
      781 gataacgcat gctcatggcc gaacattcga tgtgccagcc cggccgtccg ggtccgaaat
      841 ccgacggcca gctcggttct ttgggtttca ttgctttcca aagcacgaaa tcgcgggctt
      901 cttctttgtc gtattcgtcg ctttcgacgc gggctccgga cttgagcgtt accttgtcga
      961 gcccggccag ttttccgtat tccgggaatt tgtcgatttc gaaataaacc gaatcgtctt
     1021 tgccgcggta ggcgaatcct ttttcgacaa gggcttggac cattcgcagc atgtctttga
     1081 cgtgcttggt ggctcgggga taaaccgcgg ctttttcgat gttcaggtct ttaagatcgg
     1141 aaaaaaagat gcgggtgtag ggaagggtga tctcggaaat ggttttcgat tcctcgatgg
     1201 cttttttgat gatcttgtct tcgacgtcgg tgatgttcat cacctgtttc accttgaatc
     1261 cgttccattc gagggtgcgg cggagaaggt cttcgaagat ataggtgcgg aaattgccga
     1321 tgtggctgaa atcgtagacg gtcgggccgc agg
//
LOCUS       DAOPQT010000140         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_108179_length_1331_cov_4.337774, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000140 DAOPQT010000000
VERSION     DAOPQT010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108179_length_1331_cov_4.337774"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1331
                     /locus_tag="PKG74_03220"
     CDS             <1..>1331
                     /locus_tag="PKG74_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOB90310.1"
                     /translation="EPLFGADREDVFKVAAADFVSDEEGTGLVHIAPAFGEDDLNLAK
                     KINLKKFPTTVSDDGRMAAGFPGAGKPIKEADPEIVGDLEKRGLVYKFDSIEHEYPHC
                     WRCGTPLIYMARHSWFFEVSRLRSELVKANERVNWIPEHIKEGRFGEWIKEAKDWAIS
                     RERYWGTPIPVWKCGKCEHEEVIGGIDDLVRLSKQPTNRCFLLRHGESQSNVLNVSSA
                     APEKFDNPLTDKGRKDIESLLPRLKKEKIDLIFSSPLKRSKETSEIIAKGLQIDVKYD
                     DRLTEYRTGVFNGQPAEEFEGFFANQEEKFFKAPEQGETLTDIRARMMNFVRELNENH
                     SEKNILIVGHGDPLWALQAALKGLDERFSAEAKADLYLKPGELRKIEVRNLPFDRTGK
                     LDLHKPYIDEFKLICPDCGAEMTRTKEVADVWFDSGAMPFAGNYYPQYYPA"
BASE COUNT          347 a          329 c          368 g          287 t
ORIGIN      
        1 cgagccgctt ttcggagcgg atcgggaaga cgttttcaaa gtagcggcgg cggattttgt
       61 ttccgacgaa gaaggtacgg gattggttca tatcgctccg gctttcgggg aagacgatct
      121 gaacctcgcc aagaaaatca atctgaagaa attcccgacg accgtgagcg acgacggccg
      181 aatggctgcc gggtttcccg gagccggaaa acccatcaag gaggctgatc cggaaatcgt
      241 cggtgatctc gaaaaaagag gcttggttta caaattcgat tcgatcgagc acgaatatcc
      301 tcattgctgg aggtgcggca ctcccttgat ctatatggcg aggcactcgt ggtttttcga
      361 agtcagtcgt ctccggtcgg agcttgtcaa agcaaacgaa agagtcaact ggatcccgga
      421 gcacatcaaa gagggccgtt tcggcgagtg gatcaaagaa gccaaggact gggcgatctc
      481 gagagagcgc tattggggca cgccgattcc ggtttggaaa tgcggcaaat gcgagcacga
      541 agaagtaatc ggcggaatcg acgatctcgt caggctgtcg aaacagccga ccaaccgctg
      601 ttttctcctt cgccacggcg aatcccagtc gaatgttttg aacgtctctt ccgccgctcc
      661 ggagaaattc gataatcctt tgaccgacaa gggtcggaaa gacatcgaat cccttttgcc
      721 gcggctcaaa aaagaaaaga tcgatcttat attttcttcg cctttgaagc gttctaaaga
      781 aacgagcgag atcatcgcca aaggactcca gatcgacgtc aaatacgacg atcgcctgac
      841 cgagtaccgc accggcgttt tcaatggcca accggccgaa gaattcgaag gatttttcgc
      901 gaatcaagag gagaagttct tcaaggcgcc ggaacaaggc gagactttaa ccgacatcag
      961 ggcgagaatg atgaattttg tccgggaatt gaacgaaaat cattctgaga agaatatcct
     1021 gatcgtgggt cacggcgatc cgctctgggc tctccaggcg gcactaaagg gcttggacga
     1081 gcgcttctcg gccgaagcca aagccgacct ttatttgaaa cccggagagt tgagaaagat
     1141 cgaggttcga aatcttcctt ttgatcggac gggcaaactc gatcttcata agccttatat
     1201 cgacgaattc aaactgatct gtccggactg cggagcggaa atgacgcgga ccaaagaagt
     1261 ggccgatgtc tggttcgatt ccggggccat gccttttgcc ggcaattact acccgcaata
     1321 ttatccggcc g
//
LOCUS       DAOPQT010000141         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_108478_length_1329_cov_1.811617, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000141 DAOPQT010000000
VERSION     DAOPQT010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108478_length_1329_cov_1.811617"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKG74_03225"
     CDS             complement(<1..198)
                     /locus_tag="PKG74_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90311.1"
                     /translation="MSKKQKNEFHGGRIKTLVENLPKAGSPHTPVRSRDERWAAVGEI
                     IQQVQRITHRHHTWREHLADAS"
     gene            complement(191..1249)
                     /locus_tag="PKG74_03230"
     CDS             complement(191..1249)
                     /locus_tag="PKG74_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90312.1"
                     /translation="MENQNLIPNTTPVPHILIRQWMPRLGDVELRILLVVVDQTLGWI
                     EDPTTKRRKEKDWISHSQLAAKTGKGSASISRGLKTLISDHQLIVAVDSKGRLLDTPE
                     KRTKIGRGGKIFYRLNVHAPQLTLFDLPVPPKEKPHQIDDPSEKPHQKKHPFFDDLQK
                     KPDLTKESIKDSMRSAEPTAPENKNIVSSILEEGAKKAAEIQDHDAGLPKKKEPSVHA
                     QFMDFWYKLVLKTRGFKPQISPADGQNLKRVLQTGIKPDVLEKIAVFFLADQSLKKFA
                     PTLATMLSGGILNGLLNRMENDPEFYRNLNHYADLYLPRKENERPKEIPAVEIRSAIS
                     LLMNKFKMPGREEAQAYE"
BASE COUNT          266 a          322 c          356 g          385 t
ORIGIN      
        1 actggcatcg gccagatgtt cgcgccaggt atgatggcgg tgggtgatcc gctgcacctg
       61 ttggatgatc tcgccgactg cagcccaccg ttcgtcacgg ctgcgcaccg gtgtatgagg
      121 ggatccggct tttgggaggt tttcgacgag cgttttgatc cggccgccgt ggaattcgtt
      181 tttctgcttt ttactcatac gcttgagcct cctctctccc gggcatctta aatttattca
      241 tcaagagcga gatcgcgctc cggatctcga ctgccgggat ttctttcggc cgctcgtttt
      301 ctttgcgcgg caagtagaga tcggcgtaat gattcagatt ccggtaaaat tccggatcgt
      361 tttccatgcg attgagcagt ccgttcaaga tccctcccga gagcatagtc gcaagcgtgg
      421 gcgcgaattt cttgagcgac tgatcggcaa ggaagaacac ggcgatcttc tcaaggacat
      481 cgggctttat cccggtttgg aggactctct tgagattttg gccgtcggcc ggggagatct
      541 gcggcttgaa tccccgagtc tttaggacga gtttatacca aaaatccata aattgagcat
      601 gaaccgatgg ctctttcttt ttaggaagcc cggcatcatg atcttgaatt tcagccgctt
      661 ttttggctcc ttcttccagt attgacgaaa ctatattttt attttccggt gcggtcggct
      721 ctgccgagcg catactatct tttatacttt cttttgttaa atcgggtttc ttttgtaggt
      781 catcaaaaaa agggtgcttt ttttgatgag gcttttcaga agggtcatca atttgatgag
      841 gcttttcttt gggggggacc ggaagatcaa agagggttaa ttgaggcgcg tggacattca
      901 gccgatagaa gatcttgcct ccccggccga ttttggtccg cttctccgga gtatcaagaa
      961 gccgtccctt tgagtccacg gcgacgatca attgatggtc cgagatcagc gtctttagcc
     1021 cccgggatat agaagccgat ccctttccgg tttttgccgc caattgagaa tgcgagatcc
     1081 agtctttctc cttgcggcgc ttcgtcgtcg gatcttcgat ccatccgagc gtctgatcga
     1141 cgaccacgag aaggatccga agttcgacat ctccgaggcg cggcatccat tgacggatca
     1201 atatatgcgg aaccggagtt gtgttcggaa tgagattttg attctccatg atttaattat
     1261 agcgcatctc ggcaatagcg tcgatgcgcg attgtggata attccgggga taagtccccg
     1321 gatctgttg
//
LOCUS       DAOPQT010000142         1319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_109621_length_1319_cov_3.141614, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000142 DAOPQT010000000
VERSION     DAOPQT010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109621_length_1319_cov_3.141614"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PKG74_03235"
     CDS             complement(<1..887)
                     /locus_tag="PKG74_03235"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOB90313.1"
                     /translation="MKNNPLENARQQLFDIAKILELDKKTIDRLLKIENLVSGEIPVK
                     LDNGKIKKFKAWRSQHNNAIGPYKGGIRFHEETTEEEVKALSMWMTWKTAIIGIPYGG
                     GKGGVAVNPKTLSKSELQRLSRGYIRLIAKSIGSDKDIPAPDVNTNAEIMGWMLDEYE
                     KIIGHKDPGALTGKPLELGGSKGRTEATGLGGSYILDSFVKKLKKQKKAVSVAIQGFG
                     NVGSYFAKFVHESGYKVVAVSDSKNTLYNEKGLDIGKAFDYKAKNDAFKGFVGGKLLP
                     SEAVLGLNVDILVPAALENA"
BASE COUNT          318 a          330 c          285 g          386 t
ORIGIN      
        1 gcgttttcca aagcggccgg cacgagaata tcgacgttca agcccaaaac cgcctccgaa
       61 ggtaaaagct ttcctccgac aaatccttta aaagcgtcgt ttttcgcttt gtaatcgaaa
      121 gcttttccga tatccaatcc tttttcgttg taaagggtgt ttttgctgtc ggagaccgcc
      181 actactttgt atccgctctc gtggacgaac ttggcgaaat acgatccgac attcccgaat
      241 ccctggatgg ccacggaaac ggcttttttc tgttttttta gttttttgac gaaagaatcg
      301 agaatataag aaccacccaa acccgtggct tcggtgcgac ctttggagcc gccgagctcc
      361 aaaggcttgc ccgtgagcgc tcccggatct ttgtggccga tgattttttc gtattcgtcg
      421 agcatccagc ccatgatttc ggcgttcgtg ttcacgtccg gcgccggaat gtctttgtcg
      481 ctgccgatcg atttcgcgat caaccggata tatccacggc tcaatctttg aagctcggat
      541 ttggaaaggg ttttcggatt caccgccact ccgcctttgc cgccgccgta gggaatgccg
      601 ataatggccg ttttccaggt catccacatc gaaagcgcct tcacttcttc ttcggtcgtt
      661 tcctcgtgga atctgattcc gcccttatag ggtccgatgg cgttgttgtg ctggctcctc
      721 caagctttga attttttgat tttgccgttg tcgagcttta ccggaatctc tccggaaacc
      781 aaattctcta ttttaagcaa ccgatcgatg gtttttttat cgagctcaag gattttggct
      841 atatcaaaga gctgctgcct ggcgttttcg agcggattat ttttcataag tgatttaatt
      901 ctaaccccga ttcggaaaag ccccaaatag aaataaacta tcaaaaaagc ctttattgga
      961 gtttatttct gcctcttgac aaaacaaacc aatatatgtt gtaataaaat tacaaaaatt
     1021 gaggagcgac aaaaacgaac gctccaggtc ttcaaaagta gcgaagcgga gtctaaaaac
     1081 caggctccag ccagacggcg cgcaggttct cattcatacc aagagcggag aaagaacctg
     1141 tcgcgccgtt tccatatccg cacggatatt ttacttgcaa tctattcctt aaattgctat
     1201 tttaaaaatc tgttctcaat attttccaat atataaggag ggtcttgttg atggagtcgt
     1261 cgccccgaag aacttcgtcc aaagcctgtc tttttcccag actgcttccc aaagatcgg
//
LOCUS       DAOPQT010000143         1305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_111317_length_1305_cov_2.140800, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000143 DAOPQT010000000
VERSION     DAOPQT010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111317_length_1305_cov_2.140800"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>319)
                     /locus_tag="PKG74_03240"
     CDS             complement(<1..>319)
                     /locus_tag="PKG74_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOB90314.1"
                     /translation="RRVTRVVAGGKRFRFRATVVVGDGKGSVGLGVAKGLDVQQAVQK
                     ARTAAKKNLFRVKIQGGTIPHEVKAKASAAEVLLKPAKPGHGLLAGGAVRTVLLLSGI
                     KDIS"
     assembly_gap    320..329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(369..722)
                     /gene="rplR"
                     /locus_tag="PKG74_03245"
     CDS             complement(369..722)
                     /gene="rplR"
                     /locus_tag="PKG74_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOB90315.1"
                     /translation="MKPVKLQNQRVIRRQARTRSKIRGTALMPRLSVKRGNRNIFLQA
                     IDDVKGVTLVSASTKELDFKGTKSERAMQLGELIAEKAKKIGVSKMVFDRGAYKYHGR
                     VKQVAEGARKAGIEI"
     gene            complement(731..>1305)
                     /gene="rplF"
                     /locus_tag="PKG74_03250"
     CDS             complement(731..>1305)
                     /gene="rplF"
                     /locus_tag="PKG74_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOB90316.1"
                     /translation="RNLVINMSKIGKQPVKMIEGVKASMGDGVLKLSGPNADLEVKML
                     PGVAAEIKEDEIVFTATDRSDKTRANWGTMRALTANAVSGAAKDFSKALKIEGIGFKA
                     SVDGENLVLNLGFSHPIVLALPKGIKASVDKNIITVAGADKFLVGETAAKIRSFKKPE
                     PYLGKGVMYVDEVVRRKAGKKVAGTTAKAA"
BASE COUNT          244 a          377 c          301 g          373 t
ORIGIN      
        1 ggaaatgtcc ttgatgcccg agagaagtaa aaccgttcgg accgcgcctc cggccaaaag
       61 tccgtgtccg ggcttggccg gcttgagaag cacttcggcg gcgctcgctt tggccttcac
      121 ttcgtgtgga atggtaccgc cttgaatttt aacgcggaaa agattctttt tggccgcggt
      181 cctggctttt tgcacggcct gctgaacatc caatcctttg gccacgccga gtccgacaga
      241 ccctttgccg tcgccgacaa caaccgttgc cctgaatctg aatctcttgc ctccggcgac
      301 cacccttgtc acgcggcgcn nnnnnnnnna attcgtccct gattgttctt ctgtaaccgt
      361 tcattgtttt atatttctat gccggctttt ctcgcgcctt cggcgacctg cttcacccgg
      421 ccgtgatatt tgtaagctcc cctgtcgaaa accattttgg aaacgcctat ttttttggct
      481 ttctcggcta tcaactctcc gagctgcatg gctctttccg atttggttcc tttaaaatcc
      541 aattctttgg tcgaagccga aaccaaagtg acgcctttga cgtcgtcgat cgcctgcaaa
      601 aaaatgttcc ggtttcccct tttgaccgac aaacgcggca tcaaagccgt ccccctgatc
      661 ttgctccggg tcctcgcctg ccttcttatt actctttggt tttgaagttt gactggtttc
      721 atttggatga ttaagcggct ttggcggtcg tacccgcgac tttcttgccc gcctttcttc
      781 tcacgacttc gtcgacgtac atcacgcctt tgccgagata aggttcgggt ttcttgaacg
      841 aacggatttt agccgcggtt tctccgacca agaacttatc ggctccggcg acggtgatga
      901 tgtttttgtc gacggatgcc ttgattcctt tcggcagcgc caaaacgatc ggatgggaaa
      961 aaccgaggtt caaaaccagg ttttctccgt caaccgacgc cttgaatccg ataccttcga
     1021 tttttaaagc cttggaaaaa tctttggccg ctccggatac ggcgttcgcg gtcaaagccc
     1081 gcatcgtgcc ccagttggct cgggttttgt cggaacgatc cgtcgccgta aaaacaatct
     1141 cgtcttcctt gatttcggcc gcgactcccg gaagcatttt cacctcgagg tcggcgttcg
     1201 gcccggaaag cttcaaaacg ccgtctccca tggaagcttt cactccttcg atcatcttca
     1261 ccggttgttt tccgatctta gacatgttga ttaccaaatt tcgaa
//
LOCUS       DAOPQT010000144         1298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_112072_length_1298_cov_2.950925, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000144 DAOPQT010000000
VERSION     DAOPQT010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112072_length_1298_cov_2.950925"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PKG74_03255"
     CDS             complement(<1..422)
                     /locus_tag="PKG74_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90317.1"
                     /translation="MRKNIVILSVILLVAAAGIAFYFFDGRLRETEVLPPNVSAWKGK
                     QLVADYFEFLIEGAIQDISAETSPATVTIAVKLPSIFVDPDAETADVVCELKEDAEIT
                     FYDLSTGEETSVTLADLNVLNQVVAELDGMTNVSVLDG"
     gene            complement(469..>1298)
                     /locus_tag="PKG74_03260"
     CDS             complement(469..>1298)
                     /locus_tag="PKG74_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90318.1"
                     /translation="ASFVRFFPSINLLINLFVPLVITIVLKAGFVYAGDFLAKRNAAP
                     IVETAASKEEKRKTRIFRLKNFGYSLAMFFTVFVFFQIVSGLFSGTILASLFGHPLFA
                     FCFMIIVSGKLMNILNKNQWSKRLTILFPLLYMIYRVLLGQWQFVLGSARMVVIMWFI
                     FNVFKQAIAFYVQTREISVVPLDCLKKGDMPCPSAEIIIKSKLSVVGRVDDLGSWGPE
                     GLSLEQVTLISSIPVEERKTDVEIYKTFSFAPYMLFSAVLCILTRSSFMALLVNWIG"
BASE COUNT          391 a          324 c          285 g          298 t
ORIGIN      
        1 tcaccgtcca aaaccgagac gttggtcatt ccgtcgagtt ccgccaccac ctggttcaaa
       61 acgttcaaat ccgccagcgt taccgaggtt tcctctcccg tggataaatc gtagaaagtg
      121 atctccgcgt cttctttgag ttcgcagacg acgtcggccg tctccgcgtc cgggtccacg
      181 aaaatgctcg gcaatttgac ggcgatggtg acggtggccg gagacgtttc cgcagatatg
      241 tcctggatcg ctccttcgat caagaattcg aaataatcgg ccaccaattg tttacccttc
      301 caagccgaga cgttcggagg caaaacctcg gtttctctca agcggccgtc aaagaaataa
      361 aaagcgattc ctgccgccgc gaccaacaat atgacggaca atatgacaat gttttttctc
      421 ataaggttaa gatttatttt tatattccta attgatatta taacaaacct aaccgatcca
      481 attgaccaaa agcgccataa aagagctccg cgtcaatatg cacaaaacag cggaaaaaag
      541 catataagga gcaaaggaga acgttttgta gatctcgaca tcggttttcc gctcttccac
      601 gggaatggag gatatcagag tcacctgttc caaactcaat ccttccggac cccacgaacc
      661 caaatcgtcg actcggccga cgaccgataa tttggatttg atgatgattt cggccgacgg
      721 gcaaggcata tccccttttt tcagacaatc caacggaaca accgaaattt ccctcgtctg
      781 tacgtaaaaa gcgatggcct gcttgaaaac attgaaaatg aaccacataa taacgaccat
      841 tcgcgccgaa cccaaaacaa attgccattg tccgagtaaa acgcggtaga tcatgtataa
      901 caatggaaag agaatggtca gtcgtttcga ccattggttt ttattcagaa tattcatcag
      961 ttttccggat acgattatca tgaagcaaaa agcgaaaagc gggtgaccga aaagcgaagc
     1021 cagaatcgtc ccggaaaaca aaccggagac aatctgaaag aaaacgaaca cagtgaagaa
     1081 catcgccaaa gaatagccga agttcttcag ccggaaaata cgggtttttc ttttttcttc
     1141 tttggacgcc gcggtttcga cgatcggcgc cgcgtttctt ttcgccaaaa aatccccggc
     1201 gtagacaaac cccgctttca ggacgatcgt aatcacgagc ggaacgaaaa gatttatgag
     1261 aagattgatg gacgggaaaa atctgacaaa gctcgccg
//
LOCUS       DAOPQT010000145         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_112563_length_1294_cov_3.908797, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000145 DAOPQT010000000
VERSION     DAOPQT010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112563_length_1294_cov_3.908797"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..>1294
                     /locus_tag="PKG74_03265"
     CDS             161..>1294
                     /locus_tag="PKG74_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOB90319.1"
                     /translation="MFSKDPHIQKTIALLKEPQKLYRGMFAAVLAAGMTAVIPLIYGR
                     SIDIAVQPESDLLVIVGLLVLWFVLSLVADGLSRYSVRQSYEVAMDLTNRLLVKIFRH
                     LNDLPIRFHKEMKMGKIMRRVDRGVEDLSNMIEKSLFAFFPSVISCVVAIAILFFVEW
                     RLASILTVSSLVYVWITFVYTKNIVKKQKEMHRGWEKAYGDLWDSVLNVQAVKASVAE
                     EFEEKRNLKNFIAAGGVYIKWRNIWQEMAMWQKIVFSLGFVLVFGLGIIMLRSGELSA
                     GNLVMFFGYINLLTVPLTQLADQYRMIKSGMFSFRRAMKYYDYPEEKDVARPIVIKDL
                     KGSVEFENVDFGYKKNKLVLRDISFKVEPGESVAFVGESGVGKS"
BASE COUNT          333 a          285 c          325 g          351 t
ORIGIN      
        1 gattatttat tttatagagg cggtttcgat aaaaagcatg agatttcgtc atccgatatg
       61 ctgaaaaata ttaaaaaaat aaaagaagaa ttgtttagaa acgataaagc caatatcttt
      121 ttctgcggca atttaagcaa gctaagcaaa atttgatttt atgttttcca aagatcccca
      181 tatccaaaaa acgatcgcgc tcctgaaaga gccgcaaaag ctttatcggg gcatgttcgc
      241 cgccgttctc gccgccggga tgaccgccgt cattccttta atttacggac gctcgatcga
      301 catcgccgtc cagccggagt ccgatctttt ggttatcgtc ggactgcttg ttttgtggtt
      361 tgttctgtct cttgtcgccg acgggctttc tagatacagc gtcagacaat cttacgaagt
      421 ggccatggat ttgacgaaca ggcttctcgt gaaaattttc cgtcatttga acgatttgcc
      481 gatcaggttc cacaaggaaa tgaaaatggg aaagatcatg cgccgggtgg accggggagt
      541 ggaagatctt tccaacatga tagaaaaatc gctcttcgcc tttttcccgt cggtcatctc
      601 ctgcgtcgtc gccatcgcca ttctcttttt cgtcgagtgg cgtctggctt cgatcctcac
      661 ggtctcgagc ctggtctacg tctggatcac cttcgtctac acgaaaaaca tcgtcaaaaa
      721 gcagaaagaa atgcaccgcg gctgggaaaa agcctacgga gatttgtggg attcggtttt
      781 gaacgtccag gcggtcaaag cgtccgtggc cgaggagttc gaggaaaaga ggaatctgaa
      841 aaacttcatc gccgccggag gagtttacat caagtggagg aatatctggc aggaaatggc
      901 catgtggcaa aagatagttt tttctttggg ttttgttttg gttttcggat tgggaataat
      961 catgctccgg tccggagagc tttccgcggg caatttggtg atgttttttg gatatatcaa
     1021 tttgctgacc gtgcctttga cccagctcgc cgaccagtac cggatgataa aaagcggcat
     1081 gttttctttc cgccgagcca tgaaatatta cgattatcct gaagaaaaag atgtagcccg
     1141 tccgatcgtc atcaaagacc ttaagggatc ggtggagttc gagaacgtcg atttcggcta
     1201 caagaaaaac aagcttgttc tgcgcgacat cagcttcaaa gtcgaacccg gagaatccgt
     1261 ggcttttgtc ggagaatccg gagtgggcaa gagc
//
LOCUS       DAOPQT010000146         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_114104_length_1282_cov_2.929095, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000146 DAOPQT010000000
VERSION     DAOPQT010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114104_length_1282_cov_2.929095"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..>1282)
                     /locus_tag="PKG74_03270"
     CDS             complement(178..>1282)
                     /locus_tag="PKG74_03270"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOB90320.1"
                     /translation="DEAKTKPIQHSVRAMLSLGLQPDILVVRAKRPVEEDNLQKISLF
                     CSIPRDRVVEGLDAENIYEIPLTLEKKGLLKAVLDVFEIKKRKNGLEDWSRQYQRMSS
                     KKQVSIGLVGKYHNHPDAYISVNEALCHAGAANNVSVKIVPIDSEKPDMMEKLKTVSG
                     IVVPGGYGYRGVPGIIKAIEFSRENKVPFLGLCYGLQIAVVEWCQNVLGWKDAGSSEF
                     GLETKHPVIDILDSQKDLKKMGGTQRLGSYPAEIKKGTLLWEIYAPWHKGKGLIEERH
                     RHRFEVNPKYHREILEKGMVFGAMSPNKELVEFIELPEKMHPFFVATQAHPEFKSRFM
                     EPHPLFAAFIGAALKNSNRRTKKPKHKIKKIKR"
BASE COUNT          270 a          348 c          285 g          379 t
ORIGIN      
        1 gaaaaccatg ctttcctcgg ctgtggccat ttccgtctta tccacccttt ccggactcct
       61 tatcgcctcc aggttcgatt tgacggtcgg accgattatc gtcatcatcg caagtatcat
      121 ctttttcatc ggcttacttt ttaagcccga gtattaaaat agggccgttc tccgacccta
      181 tcttttaatc ttttttattt tatgtttggg ttttttagtt cggcgattgg aattttttaa
      241 ggccgcgccg ataaaagccg cgaaaagcgg atgcggttcc ataaatcggc tcttgaattc
      301 cggatgggcc tgggtggcca cgaaaaacgg atgcattttc tccggaagtt cgatgaattc
      361 caccagttct ttattcggcg acatggctcc gaaaaccatc cctttttcca aaatctcccg
      421 gtgatatttc ggattcacct cgaagcggtg tctgtgcctt tcctcgatca atccctttcc
      481 tttgtgccag ggcgcgtata tttcccaaag caaagttcct tttttgattt ccgccggata
      541 gctgcccagt ctctgggtgc cgcccatttt cttcaaatcc ttttgcgaat ccagaatatc
      601 gatcaccgga tgcttggttt ctagaccgaa ctcggaactc cctgcatctt tccagccgag
      661 cacattttgg caccattcga cgacagcgat ctggagtccg tagcaaagtc ccaggaaagg
      721 cactttgttt tccctcgaga attcgatggc tttgattatt ccgggaaccc cgcgatagcc
      781 gtagcccccg ggaacgacga ttcccgagac cgttttcaat ttttccatca tgtccggctt
      841 ctcggaatcg atgggaacga tcttcacgga aacgttattg gcggcgccgg cgtggcacaa
      901 tgcttcgttc accgaaatat aagcatccgg gtgattgtgg tatttgccga ccaaaccgat
      961 cgacacttgt tttttcgaag acatccgttg gtattgcctc gaccaatctt ccagtccgtt
     1021 tttccttttc ttgatctcaa agacgtcgag cacggccttc aaaaggcctt tcttttccaa
     1081 agttaatgga atctcataaa tattttcggc gtcgagtcct tccaccaccc gatcccgcgg
     1141 aatgctgcag aaaagcgaaa tcttctggag attgtcttcc tcgaccggtc gcttggctct
     1201 cacaacgaga atatccggct ggagtccaag agagagcatg gcccgcaccg aatgctggat
     1261 gggtttggtt ttcgcctcgt cc
//
LOCUS       DAOPQT010000147         1260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_117013_length_1260_cov_3.381743, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000147 DAOPQT010000000
VERSION     DAOPQT010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117013_length_1260_cov_3.381743"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..699
                     /locus_tag="PKG74_03275"
     CDS             220..699
                     /locus_tag="PKG74_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOB90321.1"
                     /translation="MVVLLHNIRSLYNTGSIFRTADAAGFSKIYLCGITPEPLDRFGR
                     YRKEISKVALGAEKYIEWEKFKSTGRLIDRLRKEGYKIWSIEQDEDSVPYDKVRVSRK
                     DKIALVLGSETKGLPKSILEKSDKIVEIPMKGRKESLNVSVAFGIVAFSLGKNNKRK"
     gene            718..1203
                     /locus_tag="PKG74_03280"
     CDS             718..1203
                     /locus_tag="PKG74_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOB90322.1"
                     /translation="MPSIKKMIFLAVIIVILIFFWIAFYYFLQDIGQIREIEASMYRG
                     GAEKATLKLETATDPISQAKGLSGRDKLQETDGMLFIFDSDEPRVFWMAGMRFSLDIV
                     WIKDNVVIGIEYDVPPYKSSEGMPEFYESPGPVDKVLELPAGEAETIDLQIGDRLVVK
                     Q"
BASE COUNT          327 a          286 c          305 g          342 t
ORIGIN      
        1 taatttcatc tagtagccac cttgagctgg tccttaattt gggagtccgg actatatgcc
       61 tagtctttcc cggactcccc gtcatctttt tttctttcat tgttccgttc cttctacctc
      121 ctactatcct gggcttgggg caggcccggg atacctttta cccacctaag acgtgggctt
      181 attttatccg gccggaaaaa tctgttataa aataccttta tggttgtctt gcttcataat
      241 atccgcagtc tttacaacac tggttcgatt tttcggacgg ccgacgccgc cggattttcc
      301 aagatttatc tctgcggcat cacgccggag ccgctcgatc gtttcggccg ataccggaaa
      361 gaaataagca aggtcgcttt gggagcggaa aaatatatcg aatgggaaaa atttaaatca
      421 accggtcggt tgatcgatcg gttgcgtaaa gaaggctata agatctggag catcgaacag
      481 gatgaagatt cggttcctta cgataaagtc cgggtttccc gaaaagacaa aatcgcttta
      541 gttttgggaa gcgaaaccaa aggtttgcca aaatccatat tggagaaatc ggacaaaata
      601 gtcgaaattc ccatgaaagg ccgaaaagaa tcgctgaacg tttccgtggc tttcggcatc
      661 gtcgcttttt ctctcggaaa aaacaataag cggaaataag gtataattag atccgtaatg
      721 ccttcgatca aaaaaatgat atttctcgcc gttattatcg ttatccttat ttttttctgg
      781 atagcttttt attattttct tcaggatatc ggtcagatcc gggaaatcga ggcgtcgatg
      841 taccgcggag gcgcggaaaa agcgactctc aagctcgaaa cggcgaccga tcccataagc
      901 caggccaagg gcctttccgg ccgcgacaag cttcaggaga cggacggaat gctttttatt
      961 tttgattcgg atgaaccgag ggtgttctgg atggcgggca tgaggttttc tctcgatatc
     1021 gtctggataa aagacaacgt cgtgatcggc atcgaatacg acgttccgcc gtataaatcg
     1081 agcgaaggaa tgccggagtt ctacgaatct cccggtccgg tggataaagt cctggagctt
     1141 ccggccggag aagccgaaac catcgatctt cagatcggcg accggctcgt cgtgaagcaa
     1201 taatatggac aaagagaaga aaataatcgg gcttcatcgc aatgttttct ttctcgggct
//
LOCUS       DAOPQT010000148         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_118458_length_1250_cov_1.753138, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000148 DAOPQT010000000
VERSION     DAOPQT010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118458_length_1250_cov_1.753138"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..425)
                     /locus_tag="PKG74_03285"
     CDS             complement(90..425)
                     /locus_tag="PKG74_03285"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOB90323.1"
                     /translation="MFTFPFSIFYFQDGTMWKNPFILFLIAVGLFFVFLPSYQRMQDL
                     KQANIEYEQRIQELIEEKEALTEEKRLLEEDPIYLEKVAREKMGLIREGEIIFKIEPR
                     TTNSETPRY"
     gene            complement(490..>1250)
                     /gene="eno"
                     /locus_tag="PKG74_03290"
     CDS             complement(490..>1250)
                     /gene="eno"
                     /locus_tag="PKG74_03290"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938675.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOB90324.1"
                     /translation="SESIRMGTEVFHNLKKLLHAKKLSTSVGDEGGFAPDLKSNEEAV
                     NVILDAIRAAGYKPGKDMYIALDPASSEFYKNGSYMLEAEAVPKKSALQMVDFYSEWV
                     DKYPIVSIEDGLAEDDWDGWKALTGRLGNKIQLVGDDLFVTNVKKLSQGIEKSIANAI
                     LIKLNQIGSVSETLDTINLAKKNKYNSVISHRSGETEDTTIAHLAVATGVGQIKTGSL
                     SRTDRLCKYNELLRIEEELGSKAIYGGTLWRKIW"
BASE COUNT          296 a          345 c          260 g          349 t
ORIGIN      
        1 tcatccacgg cctcatggcc gtggtctttt cgcgatgata aaaaaaggcg cctttcagcg
       61 ccttttttaa tctaatgatt tacgataact caataccggg gcgtttctga attcgttgtc
      121 cggggttcga tcttaaaaat gatctcgccc tcacggatca ggcccatttt ttccctggcc
      181 actttttcca aataaatggg gtcttcctct aaaaggcgct tctcttcagt caaggcctct
      241 ttctcttcta taagctcttg gatccgctgc tcgtactcta tgttcgcctg cttaaggtcc
      301 tgcatccgtt ggtacgatgg cagaaaaacg aaaaacaagc cgaccgcgat cagaaataga
      361 atgaatgggt ttttccacat agttccgtcc tgaaaataga aaatagaaaa tggaaaagtg
      421 aacatggaaa aatcaaacca gcttccatct tccctcttcc attctctatt ctcctatcaa
      481 attacaaaac taccagatct ttctccacaa tgtcccgccg taaatcgcct tggagccaag
      541 ctcctcttca atacgcaaaa gctcgttgta tttgcagaga cggtctgtcc tggacaacga
      601 ccctgtcttg atctgcccga cgccggtcgc caccgccaaa tgggcgatcg ttgtgtcttc
      661 ggtctcaccg gaacgatgag aaataacaga attgtatttg ttcttcttgg ccagattgat
      721 cgtatccagc gtttcgctaa ccgacccgat ctggttgagc ttgatcagga tcgcgttggc
      781 gatcgacttt tcaatgccct ggctcaattt tttcacgttg gtgacaaaaa gatcgtcccc
      841 gaccagttgt atcttgttcc caagcctccc ggtaagcgcc ttccaaccgt cccaatcgtc
      901 ctccgccaga ccatcctcta tcgaaactat cggatactta tccacccact cggaataaaa
      961 atccaccatc tggagggccg atttcttcgg aacagcttcc gcctcgagca tgtaggaacc
     1021 gttcttgtaa aactcgcttg aagccggatc gagcgcaatg tacatgtcct tcccgggctt
     1081 gtagccggcg gcccttatcg cgtcgagtat tacgttgaca gcttcctcgt tcgatttgag
     1141 gtccggagcg aatccgcctt cgtcaccgac cgatgtagaa agttttttcg cgtgaagaag
     1201 cttctttaga ttatggaata cttccgtacc catccttatg gactcgctga
//
LOCUS       DAOPQT010000149         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_118839_length_1247_cov_2.627517, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000149 DAOPQT010000000
VERSION     DAOPQT010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118839_length_1247_cov_2.627517"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PKG74_03295"
     CDS             <1..1025
                     /locus_tag="PKG74_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOB90325.1"
                     /translation="SAYGPTKNPHDLERVPGGSSGGSAAAVAADMAVVALGSDTGGSI
                     RQPAGFCGTVGFKPTYGAVSRFGLLAMCSSLDQIGPFARSVKDAEMVFEEIKGEDRYD
                     ATSFDLPDRRLWEKEDLKNIVIGLPKEYLEGGLSKEVETTIKNVIHEFTDMGAVFKDI
                     SLPHTKYALPCYYIIMPAEASTNLARYDGIRYGRSKDAKSLLEIYEKTKGERFGEEVK
                     RRILLGTFVLSSGYYDAYYAKAQRVRRLIREDFEKAFDSDKVDAILAPVSPSPAFKIG
                     EKTQNPMEMYLSDIFTIPANLAGIPGLAIPAKNKEGDLPIGFQLMGKWGDDKNLFSLG
                     KLYESI"
BASE COUNT          321 a          310 c          328 g          288 t
ORIGIN      
        1 cttcggccta cggcccgacc aaaaaccccc atgatctgga gcgggtgccc ggaggttcgt
       61 ccggcggctc ggcggcggcg gtcgcggccg acatggccgt ggtcgccttg ggatcggata
      121 ccggaggctc gatcaggcag ccggccggat tctgcggcac agtcggattc aaaccgactt
      181 acggcgcggt ttcgcgtttc gggcttttgg ccatgtgttc gagtctggat cagatcgggc
      241 cttttgcgag atcggtcaaa gacgccgaaa tggttttcga agagatcaaa ggcgaagacc
      301 gctacgacgc cacttctttc gatttgccgg acaggaggct ttgggaaaaa gaagatttga
      361 aaaatatcgt catcggtctt cccaaagaat acctggaagg agggttgtcg aaagaagtcg
      421 aaacgacgat caaaaacgtc atccacgagt tcacggatat gggggcggtt ttcaaggaca
      481 tcagcttgcc gcacaccaaa tacgccctgc cttgctatta catcattatg ccggccgagg
      541 cctcgaccaa tttggctcgt tacgacggca tacgttacgg ccgatcgaaa gacgcgaaaa
      601 gccttctcga gatttacgag aaaaccaaag gcgaaagatt cggcgaagaa gtcaaacgcc
      661 ggatactcct cggaactttc gtgctttctt ccggctacta tgatgcctat tacgccaaag
      721 cccaaagagt gagaagattg atcagagagg atttcgaaaa agctttcgac agcgacaaag
      781 tggacgcaat tctcgctccg gtttcgccgt ctccggcttt caaaatcgga gaaaagacgc
      841 aaaatccgat ggagatgtat ctttccgaca ttttcaccat tccggcgaat ctggccggaa
      901 tccccgggct cgctattccg gccaaaaaca aagaaggaga tttgccaatt ggatttcaat
      961 tgatggggaa gtggggagac gacaagaatc ttttctcctt ggggaaactt tacgaaagta
     1021 tataaaataa atatgaaaaa cccgcacatc cttgtgcggg ggtcccaata ggtccgttat
     1081 aagggatcgg taatgaagat gtttagttcg agccattccc gaacttcacc ttcacctttt
     1141 ccgatgcatc ttatacggcc gtaaagaact gcctctttgc cattgagaag atcgaaatct
     1201 tctcgatcta ctttccgatt gataaccgtg actttcgttg ctgcggt
//
LOCUS       DAOPQT010000150         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_119433_length_1243_cov_1.877946, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000150 DAOPQT010000000
VERSION     DAOPQT010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119433_length_1243_cov_1.877946"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PKG74_03300"
     CDS             <1..641
                     /locus_tag="PKG74_03300"
                     /inference="COORDINATES: protein motif:HMM:NF025044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOB90326.1"
                     /translation="KTWFLSRTWTSFMIIPELIKRFVDVFAELPGIGPRQAIRLAFYL
                     INRGKGFNEQVSSAVDSLKEITLCKNCFNITRNQSGLCDICENPKRDKAIIAIVEKET
                     DLMSMENTKKWNGVYLILGDLKKTGVLDAGQKNRIEVLKDRIVRDLGGQAKEIIIAFN
                     PTTYGDINSQIVGKELRPLTEKISRLGRGIPTGGEIEFADEETLSAAIERRD"
     gene            complement(666..965)
                     /gene="rplU"
                     /locus_tag="PKG74_03305"
     CDS             complement(666..965)
                     /gene="rplU"
                     /locus_tag="PKG74_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009752820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOB90327.1"
                     /translation="MLSVIKTGGKQYVVSSGDKIKIEKLEAEPGQEVVFDKVLFQEKD
                     GQVEIGRPYISGASVKAKVARQARDKKIIVFRYHSKTRYRKTKGHRQHFTEVVIA"
     gene            complement(976..>1243)
                     /locus_tag="PKG74_03310"
     CDS             complement(976..>1243)
                     /locus_tag="PKG74_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90328.1"
                     /translation="AAMGIIFVAAIVNILGPAGNFVVSTGIIGDKTTTAEIIGVGFVV
                     VLMNSWLSFVVFRKERLISWFLAMTGLVVAILTLIKVASVVYFW"
BASE COUNT          330 a          332 c          305 g          276 t
ORIGIN      
        1 caaaaacctg gtttctttcg agaacgtgga catcattcat gataatcccg gaattaatta
       61 aacggttcgt cgacgttttc gccgaactgc cgggcatcgg cccgaggcag gccatccgct
      121 tggcttttta tttgatcaat cgcggcaaag gattcaacga acaggtttcc agcgccgtcg
      181 attctttgaa agagatcact ctctgcaaga actgcttcaa tatcacccgg aaccaaagcg
      241 gactctgcga catctgcgaa aaccccaaac gggacaaggc gatcatcgcc attgtcgaga
      301 aagaaaccga tttgatgtcc atggaaaaca ccaagaagtg gaacggcgtc tatctaatcc
      361 tcggcgacct caaaaaaacc ggcgtcttgg acgccggaca gaaaaaccgg atcgaggttc
      421 tgaaagaccg gatcgtgaga gacctcggcg gccaagccaa ggaaatcatc atcgctttca
      481 atcccacgac ctacggagac atcaatagcc aaatcgtcgg caaagagctc cgacctttga
      541 cggaaaaaat cagccgcttg ggccgcggca ttccgaccgg aggcgaaatc gaattcgccg
      601 acgaagaaac cctttccgcc gccatcgaac gtcgagactg aaaatccccc tttgggggat
      661 ttttatcaag ctatgacgac ttcggtgaag tgctggcggt gtcctttggt cttccgatag
      721 cgggttttgg agtgatagcg gaaaacaatt atctttttgt ctctcgcctg ccgggccact
      781 ttggctttga ccgaagcgcc ggagatatag ggccggccga tttccacttg gccgtccttt
      841 tcctggaaca gaactttatc gaaaaccacc tcttgtccgg gctcggcttc caatttttct
      901 atcttgattt tgtctccgga agaaacaacg tattgttttc cgccggtttt gatgactgat
      961 aacataggcc aaatactacc agaaatagac caccgacgca acctttatca aagtcagaat
     1021 ggcgaccaca aggccggtca tggccaaaaa ccaggaaatc agcctttctt tcctgaagac
     1081 gacgaaagac agccaggagt tcatgagcac gacgacaaat ccgacgccga tgatttccgc
     1141 ggtcgtggtt ttgtctccga tgatgccggt cgaaaccacg aaattgccgg ctgggccgag
     1201 aatgttgacg atggcggcga caaagataat tcccatggcc gcc
//
LOCUS       DAOPQT010000151         1191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_126905_length_1191_cov_3.600352, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000151 DAOPQT010000000
VERSION     DAOPQT010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126905_length_1191_cov_3.600352"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PKG74_03315"
     CDS             complement(<1..700)
                     /locus_tag="PKG74_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOB90329.1"
                     /translation="MDKTKRQFSLFKFAAAFAISFTIFIAGFAFSERLSRSRLEDLQN
                     DVLALQSEWNSLEMLTLIDKTSSVSCEMYEEQILNFTNETDVFGQKLESVANRLPTDN
                     PQVVNMKVSYWLMEIRDFFTLKQIETKCGKEYTTILYFFDRACVDCVYQGEVLRQIKQ
                     EYPEIMIYSFDVSNKDISIIKILSQIYNIKTTPTLIIGDRKIEKETYRQELNSILGIE
                     SKPLIDALKPESKTP"
     gene            complement(704..>1191)
                     /locus_tag="PKG74_03320"
     CDS             complement(704..>1191)
                     /locus_tag="PKG74_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90330.1"
                     /translation="ALKNQRVRWNRGTIRNFVKYRKFFANPVYGDFGLFIFPSTVVLL
                     SVAFGLFLVSAANLMVIAAKKFEYGTLFHVPPLSFDNFFRFDRFFIQPTAMQIISLVI
                     FGLSLVSLYFIRKYSPKDFKNMTLFYVPYLLVYYPLFVLGFWAITVFYELTHRKLAWH
                     K"
BASE COUNT          309 a          244 c          327 g          311 t
ORIGIN      
        1 ccggggtttt gctttcgggt ttcaaggcgt cgatcaacgg cttggattcg attcccaaaa
       61 tagaattcaa ttcttggcga taggtttctt tttctatttt ccgatcgccg atgatcaaag
      121 tcggcgttgt tttgatattg tagatttggg aaaggatttt gatgatggag atgtctttat
      181 tcgagacgtc gaaggaataa atcatgattt ccggatattc ctgcttgatc tgccgaagca
      241 cttctccctg gtagacgcaa tctacgcagg cccggtcgaa aaaataaaga atggtcgtgt
      301 attccttacc gcactttgtt tctatctgct tcagggtgaa aaagtcgcgg atctccatca
      361 gccagtagct cactttcatg ttgaccacct gggggttgtc ggtcggcaag cggttcgcca
      421 ccgattcgag tttctggccg aaaacgtcgg tttcgttggt gaagttcaaa atctgctcct
      481 cgtacatttc gcagctgacg ctcgaagtct tgtctatcag ggtaagcatc tcgagggagt
      541 tccattcgga ttgaagggcg aggacatcgt tttggagatc ttctagccgc gagcggctca
      601 acctttcgct gaaagcgaat ccggcgatga aaatggtaaa ggaaatggca aaggccgcgg
      661 cgaatttgaa cagactgaat tgtcttttgg ttttatccat gggttatttg tgccaagcga
      721 gttttctgtg agtcagttcg tagaaaacgg tgatggccca gaatccgagc acgaaaagag
      781 ggtaataaac gagaagatag ggaacgtaga acaaggtcat gtttttgaaa tccttgggcg
      841 aatatttacg gatgaaatat agcgaaacga gcgacaggcc gaaaataacg agggaaatga
      901 tctgcatggc cgtcggctgg ataaagaatc tgtcgaaccg gaaaaagttg tcgaaactaa
      961 gcggcggcac gtggaagagc gtaccgtatt cgaatttctt ggcggcgatg accatgaggt
     1021 tcgcggccga gacgaggaac agtccgaagg cgacgctaag aagaacgacg gtcgacggaa
     1081 aaatgaataa tccgaaatct ccgtataccg gattcgcgaa aaacttgcgg tatttgacga
     1141 aattgcggat ggtgccgcgg ttccacctga ccctttggtt tttcagagcc t
//
LOCUS       DAOPQT010000152         1189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_127202_length_1189_cov_3.012346, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000152 DAOPQT010000000
VERSION     DAOPQT010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127202_length_1189_cov_3.012346"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKG74_03325"
     CDS             complement(<1..249)
                     /locus_tag="PKG74_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOB90331.1"
                     /translation="MAKKDKIWKSVIFTGYECNNHCRFCMEQDKRNLPIRTAMEVKKE
                     IAAAKKRGSTYLELIGGETTIRPDFADIVGFAKKVGFKT"
     gene            complement(233..>1189)
                     /locus_tag="PKG74_03330"
     CDS             complement(233..>1189)
                     /locus_tag="PKG74_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90332.1"
                     /translation="QRLAEKEGYGAPYDPDVFLPMNDASAWAFEKIGEEPGLKKSYRA
                     KDYRKILGVFGAGRFSMSTIPSKISYQMMAPIDRKPTEYSCSARTYSPQMEMAERVIS
                     SLCYNHYVDCLRFEKDFQEDFFKVFYNALENLKALEAVEVKDDRVYLLTRNPRKKAAY
                     MMFFCDPDENVLRRIEERRNLASDEEWGKERGIGWLRRPRPDKRDLKKQSALKSELDK
                     LCGSFEGKTAIIDGKVTNVGKRSFRILSKEGKNFEFLLTPQTAIAEMFFDDKGESYEK
                     SATLKEIRKGDPVSVTAALGDKPQALVIRKITVVNWIDGKER"
BASE COUNT          250 a          309 c          264 g          366 t
ORIGIN      
        1 ggtcttgaat ccgactttct tggcgaaccc gacgatgtcg gcgaaatctg gcctgatggt
       61 cgtttctcct ccgataagct cgagataagt cgatcctctt ttcttggccg cggcgatttc
      121 ttttttgact tccatcgccg ttcttatcgg caggttcctc ttgtcctgct ccatgcaaaa
      181 acggcaatgg ttgttgcatt cgtatcccgt gaagatgacg cttttccaaa ttttatcttt
      241 ctttgccatc gatccaattg actacggtta ttttccttat gacaagcgcc tgcggtttgt
      301 cgcctaaggc ggccgtcacc gaaaccggat cgccttttct gatttctttc agtgtcgctg
      361 acttttcgta agattcccct ttgtcgtcga aaaacatctc cgcgatcgcc gtttgcggag
      421 tcaagagaaa ctcgaagttt tttccctcct tcgacaaaat ccggaaagat ctcttgccga
      481 cgttcgttac ttttccgtca atgatcgcgg tttttccttc gaaagaaccg cataatttat
      541 cgagttcgga tttcaaggcg gattgctttt tcaaatcccg tttgtcgggt cgcggccttc
      601 ttagccagcc gatcccgcgt tctttacccc attcctcgtc cgacgccaag ttgcggcgtt
      661 cttcgatgcg gcgcaagacg ttttcgtcag gatcgcagaa aaacatcata taagccgctt
      721 tttttctcgg attcctcgtc aaaagataaa cgcggtcgtc ttttacttcg accgcctcca
      781 aggctttaag gttctccaag gcgttataga aaactttgaa aaaatcttct tgaaagtctt
      841 tttcgaatct aaggcaatcg acataatgat tgtagcaaag cgaggaaatc actctttcgg
      901 ccatttccat ttgcggcgaa tatgtcctcg cggaacagga gtattctgtc ggttttcggt
      961 cgatgggggc catcatctgg taggatattt tggaaggaat ggtcgacata ctgaaccgac
     1021 cagctccgaa aacacccaat attttccgat aatctttggc ccggtatgat tttttgaggc
     1081 cgggttcctc tccgattttt tcgaaagccc aggcactcgc gtcgttcatg ggtagaaaca
     1141 catccgggtc gtacggagcg ccatagcctt ctttttccgc caatcgctg
//
LOCUS       DAOPQT010000153         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_130688_length_1167_cov_3.169065, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000153 DAOPQT010000000
VERSION     DAOPQT010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130688_length_1167_cov_3.169065"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..>1167
                     /gene="nusA"
                     /locus_tag="PKG74_03335"
     CDS             175..>1167
                     /gene="nusA"
                     /locus_tag="PKG74_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOB90333.1"
                     /translation="MDLKQLLTVVGQVAKEKGIEPEKIISVIEESIAAAYRKEYGKRG
                     EIIKAKLDQKSGEMDFWQVKEVVDESTVRIVSDEDQEEQSIEQKTEDEEPKLPRYNPE
                     RHIFIEEAKKVNPEVKVGDNLEFSLEPKTDFGRIAAQAAKQVILQKLREAEKDMLLQE
                     FKDKEGKVVSGIIHRFERGNVYVEIGKALGIMFGNESIPGEHYRVNDRMRFYVLSIQE
                     DRRGQPGIILSRSHPRFINRLFELEVPEINDGTVEIKGVAREPGSRTKIAVASKVPGV
                     DPVGSCVGQRGTRVMAVTNELGGQEKIDIIEWSEEPEKFVAAALSPAKVLSVEIL"
BASE COUNT          347 a          272 c          322 g          226 t
ORIGIN      
        1 agtatgaagt caggctctgt ttcaagagct tccgctcggg atttaacttg acaaacgtaa
       61 ttcgacgagt atactgattg acattaaccc ccgctctgcg ggggtttata aataaaacga
      121 gcgaaagtaa aataagataa agtcttcgtt cggagacaaa acataaaaaa caaaatggac
      181 ttaaagcaac tgttgacggt cgtcggtcag gtggccaagg aaaaaggcat cgagccggaa
      241 aaaataattt cggtcatcga agaatcgatc gccgccgcct atcgtaagga atacggcaaa
      301 agaggagaga tcatcaaagc caagctcgac cagaaatccg gagaaatgga tttctggcag
      361 gtgaaggagg tggttgatga aagcaccgtg agaatcgtct ccgacgaaga tcaggaagaa
      421 cagtcgatcg aacagaaaac cgaagacgaa gagccgaagc ttccccggta taatccggaa
      481 aggcatattt ttatagaaga ggcgaaaaag gtcaatcccg aggtcaaagt cggcgacaat
      541 ttggagttct ccctcgaacc caaaaccgat ttcggccgca tcgccgccca ggccgccaaa
      601 caggtgattc tccaaaagct ccgcgaagcg gaaaaagaca tgcttctcca ggaattcaaa
      661 gacaaggaag gcaaagtcgt ttccggcatt atccatcgtt tcgagagagg caatgtttac
      721 gtggaaatcg gcaaggcttt gggcatcatg ttcggcaacg aatccattcc cggagagcat
      781 tatcgggtga acgaccggat gcggttctat gtcttgagca tccaggaaga ccgccgcggc
      841 cagccgggaa tcattctttc ccggtcgcat ccgcgcttca tcaacaggct tttcgaattg
      901 gaggttccgg agatcaacga cggaaccgtg gaaataaaag gagtggcccg tgaacccggc
      961 agtcgcacca agatcgccgt cgcttccaaa gttccgggag tcgatccggt cggttcttgc
     1021 gtcggacaaa gagggaccag ggtgatggcg gtgacgaacg aactcggagg acaggaaaag
     1081 atcgacatca tcgaatggtc cgaagagccg gaaaagttcg tggccgccgc cctttctccg
     1141 gccaaggtct tgagcgtgga gatattg
//
LOCUS       DAOPQT010000154         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_130735_length_1167_cov_2.394784, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000154 DAOPQT010000000
VERSION     DAOPQT010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130735_length_1167_cov_2.394784"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKG74_03340"
     CDS             <1..238
                     /locus_tag="PKG74_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOB90334.1"
                     /translation="LPYVLQLLGMVSLNFTVVNLLPIPALDGGNIAFLVYEKIRGKPA
                     NAKIASTVNLAGYLILLFLMIVVNIRDISKLFLK"
     gene            235..>1167
                     /locus_tag="PKG74_03345"
     CDS             235..>1167
                     /locus_tag="PKG74_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase"
                     /protein_id="HOB90335.1"
                     /translation="MKQSELFTKTLREAPKDEESKNASLLIRGGFIYKVMAGAYAFLP
                     LGLRVFRKLEEIIREEMTSIGAEEILMTSLQPKANWETTGRWLDFDALFKIKSQISEI
                     DYALAPTHEEELTPIAKKHIVSYRDLPKAVFQIQNKFRDEKRAKAGLLRGREFFMKDL
                     YSFHADAADLDRYYDKVSKTYMKIWKRVGLGKITYFTWASGGTFSKYSHEFQTLSESG
                     EDTIYVCEKCRVAVNEEIIAEQRECPICGGREFSKKKSIEVGNIFKLGVKFSKPFELR
                     YKDKSGREIEVQMGCYGIGLNRVMGTIADTSNDEK"
BASE COUNT          329 a          264 c          289 g          285 t
ORIGIN      
        1 tcttccttac gtgctccagc tcctcgggat ggtttccttg aatttcacgg tggttaatct
       61 tctgcccatt ccggctttgg acggcggcaa catcgctttc cttgtttacg aaaaaatccg
      121 cggcaaaccg gccaacgcca agatcgcttc gaccgtcaat ttggcgggat atttgatttt
      181 gctgtttctg atgatagttg ttaacataag agacataagt aaattattct tgaaatgaaa
      241 caaagcgaac ttttcacgaa gactttgaga gaagcaccga aggacgagga atcgaaaaac
      301 gcctcccttt tgataagggg aggttttatt tacaaagtga tggccggagc ctacgccttt
      361 cttcctttgg gacttcgcgt tttcaggaaa ttggaagaga ttatccgtga ggaaatgaca
      421 tcgatcggag ccgaagaaat actgatgact tcgctccagc ccaaagccaa ctgggaaacg
      481 accgggcgct ggctagattt cgacgcgctt ttcaagataa aaagccaaat ttcggaaatc
      541 gattacgcct tggcgccgac ccatgaagaa gaactgactc cgatcgccaa aaaacatatc
      601 gtttcctacc gtgatctgcc gaaagcggtt ttccagatac aaaacaagtt ccgcgacgag
      661 aaaagagcca aagccggatt gctccgcgga cgggaattct tcatgaaaga cctttattct
      721 ttccatgcgg atgcggccga cctcgaccgg tattacgata aggtgtcgaa gacctatatg
      781 aagatttgga agcgggttgg tttgggaaag atcacttatt tcacctgggc ttcgggcgga
      841 acattttcca aatattctca tgagttccaa accctttccg aatccggaga agacacgatc
      901 tatgtctgcg aaaaatgccg ggtggccgta aacgaggaaa tcatcgccga acagcgggaa
      961 tgtccgatct gcggaggccg cgaattttcc aagaaaaaat ccatcgaagt cggcaatatt
     1021 ttcaagctcg gtgtcaagtt ttcaaagccc tttgaattgc ggtataaaga caaaagtggc
     1081 cgggaaatag aggttcaaat gggctgctac ggtatcggtt tgaacagggt gatgggaacg
     1141 atcgccgaca cctcgaatga cgaaaaa
//
LOCUS       DAOPQT010000155         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_132519_length_1156_cov_2.164396, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000155 DAOPQT010000000
VERSION     DAOPQT010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132519_length_1156_cov_2.164396"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKG74_03350"
     CDS             complement(<1..551)
                     /locus_tag="PKG74_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOB90336.1"
                     /translation="MTIDLNYLILSAIIAAAAGLIGCFALMKKMTLASDAFGHIALPG
                     LGLAILFKINPILGGAAALLIGAFLIWLIENKTKISSEAIIGVIFSASLAIGSLITPE
                     EHLIEALFGGFQEINTLELAIAAGISLAIIAFILRNKEKLVISLISQDLAVTSGIDIK
                     KLNLYFILFFGLTVILGLRYSGAL"
     gene            complement(535..>1156)
                     /locus_tag="PKG74_03355"
     CDS             complement(535..>1156)
                     /locus_tag="PKG74_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOB90337.1"
                     /translation="EGSSLAVIGPNGAGKTLLFKALIKAEAYSGEVKWKPGVKIGYIP
                     QKLDLERDLPVTAFELLSAKAKIAGLGLRKVEEAIELVDLSLADSKQPIGNLSGGQFQ
                     RTLIAFALIGDPNVLLFDEPTAGVDAPGQNKIYELINRLKKEKNLTVLLISHDLSLVY
                     EYADEVLCLNREKMCFGEPRKVLEPHALEELYGSPQKYHIHDDHRS"
BASE COUNT          288 a          281 c          311 g          276 t
ORIGIN      
        1 agcgctccgg aatacctgag gcccaaaata accgtcagtc cgaagaacaa gatgaaataa
       61 aggttcagtt ttttgatgtc gatgcctgag gtgacggcaa gatcctggga aatcaaggaa
      121 atcaccagtt tctctttgtt tctgagaata aaagcgatga tggccaagct gatgccggcg
      181 gcaatcgcca attccaaagt attgatctct tggaatccgc cgaaaagcgc ttcgatcaga
      241 tgttcttccg gcgtgatcag actaccgatg gcgaggctcg ccgaaaaaat aactccgata
      301 atggcctcgg aagaaatctt ggttttgttt tctataagcc agataaggaa ggcgccgatc
      361 aaaagggccg cggcgccgcc gagaatgggg ttgatcttga aaagaatggc gagccccaat
      421 ccgggcaggg cgatgtggcc gaaagcatcc gaagccaggg tcattttctt catgagggcg
      481 aaacagccga taagcccggc ggccgcggcg atgatggcgg aaagaataag ataattaaga
      541 tcgatggtca tcgtggatat gatatttttg cggcgacccg tacaattctt cgagagcgtg
      601 cggctccaag actttccgcg gttccccgaa gcacattttt tcgcgattca aacaaagaac
      661 ttcgtcggcg tattcgtaga caaggcttag gtcgtgggaa atgagaagaa cggtcaggtt
      721 tttctctttt ttcagacgat tgatcaattc gtagattttg ttctggcccg gcgcgtccac
      781 tccggcggtc ggctcgtcga acaaaagaac gttcggatcg ccgatcaagg caaaggcgat
      841 aagcgtccgt tggaattgtc cgccggaaag attgccgatc ggctgtttcg agtcggccaa
      901 agaaagatcc accaactcga tggcttcttc gacttttcgc aagccaagtc cggcgatttt
      961 cgccttagcc gacaggagct cgaaagccgt caccggcaga tctctttcca aatcgagttt
     1021 ttgggggata tagccgattt tgactccggg tttccatttt acttcgccgg aataggcttc
     1081 ggctttgatc aaggctttga aaagcagcgt tttccccgcg ccgttcggcc cgatgaccgc
     1141 caaactgctt ccttct
//
LOCUS       DAOPQT010000156         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_132645_length_1155_cov_2.669091, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000156 DAOPQT010000000
VERSION     DAOPQT010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132645_length_1155_cov_2.669091"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PKG74_03360"
     CDS             complement(<1..1108)
                     /locus_tag="PKG74_03360"
                     /inference="COORDINATES: protein motif:HMM:NF036827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOB90338.1"
                     /translation="MPYSVETGGGFIGFLLTALFNYATHVGAIVISVVFLIVGLGLIL
                     NRQVKKVYLAIKDVRLKARAKRAYFQAVKKEKPDKFDDIEFKEPIKTEEEKPLEKEVP
                     VIKQERQLYNTPRLEPKSEPLPSTKHEPVLEVKPTPKFEPRSDAKPVVQPSTAQALPP
                     YRPGHGDVAFQVKSFSANQGLRKAKFYLDGEQPSQEPPAAYEAVINTNPIPESKPVFE
                     PLIEHPIIAPKEESLDVKKPELEPKVKPSDKPKVEEISKPEVKIREYTMPSLDLLDNH
                     ESEADLMKNEESTASRTELINTIFEDLNIGAKVVGHTIGPSVTRYDIQMDRNVSVSSM
                     ARYINDISIRLGGVATRFEALVIGKATSGLEIPNE"
BASE COUNT          258 a          258 c          306 g          333 t
ORIGIN      
        1 attcgttcgg aatttctaaa cccgatgtcg ctttgccgat aacgagggct tcaaagcgcg
       61 tcgcgacgcc gccgagacga atcgatatat cgttgatata acgcgccatc gaagaaaccg
      121 agacattgcg gtccatctga atatcgtagc gggtgaccga tggtccgatg gtgtgcccga
      181 cgactttcgc cccgatattt aagtcttcga aaatcgtatt gatcaattcg gtgcgcgaag
      241 ctgtcgattc ttcattcttc atcaaatctg cttcggactc gtgattgtcg agcaaatcaa
      301 gtgaaggcat cgtatattcg cgaatcttga cttctggctt acttatttct tcaacttttg
      361 gtttgtcgga aggttttact ttcggttcga gttccggctt cttaacatct aaagattctt
      421 cctttggagc gatgatcgga tgttcgataa gcggctcgaa aacgggtttg ctctccggaa
      481 tcggattcgt attgatgacc gcttcatacg ccgccggagg ctcctgtgat ggttgttcgc
      541 catcgagata aaacttcgct ttgcgaagac cttgattggc gctgaaagat ttaacttgaa
      601 aggcgacatc gccgtggccg ggacgataag gcggcagagc ctgggcggtc gacggctgaa
      661 cgactggttt ggcatcgctt cgcggttcaa attttggtgt tggcttgact tctagaaccg
      721 gctcgtgttt ggtcgagggt agaggttcag actttggctc gagacgcgga gtattatata
      781 gctggcgttc ctgcttgatg accggaactt ctttttcgag aggtttttct tcttccgttt
      841 tgatgggttc tttgaactca atatcatcga atttatcggg cttttctttc ttcacggctt
      901 gaaagtaggc tcttttggct ctggctttta gacgaacgtc tttaatcgcc aggtacacct
      961 tcttgacttg gcggttcaaa attaagccga ggccgacgat gagaaaaacg acgctgatga
     1021 cgatggcgcc gacgtgcgtc gcgtaattaa aaagggcggt cagaagaaaa ccgatgaaac
     1081 caccaccggt ctcgacgcta taaggcatgc taccggggtt taagatgacc gattggatat
     1141 tgctttgaaa aacgg
//
LOCUS       DAOPQT010000157         1147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_133987_length_1147_cov_2.678571, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000157 DAOPQT010000000
VERSION     DAOPQT010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133987_length_1147_cov_2.678571"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1147)
                     /locus_tag="PKG74_03365"
     CDS             complement(<1..>1147)
                     /locus_tag="PKG74_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOB90339.1"
                     /translation="ISKLPGFGKKSEENILKGVFFAKKFRGRYILGDKLQWIESVAKD
                     LKTLKEIKKLDVAGSIRRRKETVGDLDILAVSSKPKPVMDYFVSRPWVVRVLGKGEAK
                     SSVKAENGMDVDLRVLEEKSYGAGLLYFTGSKEHNIRLRELAIKKGLKLNEYGLFQGK
                     KMIAGKTEEEVYKALGLEYVPPEMREDTGEIQLAALKKIPKLVDYGSLEGDLQIQTDW
                     SDGENSIEEYAAAAINLGLKYSAITDHTQSLKVANGLDEKRLEKQMLEIDRLNKSFDK
                     KRIDFRILKGAEVDILKDGSLDLPDKVLSKLDIVGVAIHSHFNLPEKEQTKRLIRAME
                     NKWSDIVFHPTGRLIGEREPLRLDMEAVIKAAKTIGTVLEIDSYPNRL"
BASE COUNT          236 a          316 c          245 g          350 t
ORIGIN      
        1 agtctgttcg gatagctatc tatttcgaga acagttccga tcgtcttggc cgcctttatc
       61 accgcctcca tatcgagccg aagcggctcc ctttctccga taagccgtcc ggtcggatgg
      121 aaaacaatgt cgctccattt attctccatc gccctgatca atcttttggt ctgctctttt
      181 tccggcaaat taaaatgcga atggatggcc acgccgacga tatcaagttt agataacacc
      241 ttatccggca aatccaaaga accgtctttc aggatatcga cttccgcgcc tttcaggatc
      301 cggaaatcga ttctcttttt atcgaagctt ttgttcagcc ggtcgatctc cagcatctgc
      361 ttttcgagtc ttttttcgtc gaggccgttc gcaaccttaa gactctgggt gtggtcggtg
      421 atggcgctgt atttcaaacc aaggttgatg gccgccgcgg cgtattcttc gatcgagttt
      481 tctccgtcgc tccaatcggt ctggatttga agatctccct cgagcgatcc gtaatccacg
      541 agtttcggta ttttcttcag cgcggccagc tggatctcac ccgtatcttc cctcatttcc
      601 ggcggaacgt attccagacc gagcgctttg tagacttcct cctctgtttt tccggcgatc
      661 attttctttc cctggaaaag accgtattcg ttcagcttca atcctttttt gatagccagt
      721 tcgcgaagcc ggatgttatg ctctttagag ccggtgaaat acaaaagccc ggcgccgtaa
      781 gatttctcct ccaaaaccct taaatcgaca tccataccgt tttccgcttt gaccgaggat
      841 ttggcttctc cttttcccaa aaccctgacg acccaaggcc ggctcacgaa ataatccatc
      901 accggcttcg gtttcgagga aacggccaag atatcgagat cgccgacggt ttctttccgt
      961 cgcctgatcg atccggcgac gtcgagtttt tttatttctt tgagagtttt cagatcctta
     1021 gccacggatt cgatccattg gagcttgtct ccgaggatat aacggccgcg gaactttttg
     1081 gcaaaaaaaa ctcccttcag aatattttct tcgctttttt tcccgaatcc agggagtttg
     1141 cttattt
//
LOCUS       DAOPQT010000158         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_140837_length_1109_cov_2.539848, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000158 DAOPQT010000000
VERSION     DAOPQT010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140837_length_1109_cov_2.539848"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PKG74_03370"
     CDS             <1..855
                     /locus_tag="PKG74_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOB90340.1"
                     /translation="LNICLLVLVFTPLGVQDKTSSRWLEIKGVSIQPSELLKITFIIY
                     ISSWLASAKRDRKTDVWEGFLPFLLISGLVGFLLVIQPATSIVLILMAATLIVYFVSG
                     AKLSFIAAITMLALIAFGLLIAFTPYRLARFVTFFNKDADPLGASFQVNQAMIAIGSG
                     RIFGVGYGNSTSKIIGLPEPISDSIFAIAAEEFGFVGVVFFILLFFVLTLAGFFGSLE
                     TKTKFGKFAMIGFSSIIGIQAFVNMAAISGMIPLTGTPLPFISYGGTSLTVFMTMLGL
                     MVNFYRHA"
     gene            848..>1109
                     /locus_tag="PKG74_03375"
     CDS             848..>1109
                     /locus_tag="PKG74_03375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB90341.1"
                     /translation="MRKLRIAFAGGGTGGHVYPLLAVATETQILAANKRLEIEMRYFG
                     SPKQYRTTLEENGISVSSIMESKIRRYFDLRNLIDIPKFGLSL"
BASE COUNT          233 a          308 c          264 g          304 t
ORIGIN      
        1 ctaaacatct gccttctcgt gctcgttttc acgcctctcg gagtccagga caaaacttct
       61 tcgaggtggc tcgagatcaa aggagtctcg atccagccgt cggaacttct taaaataact
      121 tttatcatct atatctcttc ctggctcgcc tcggccaaac gcgaccggaa aacggacgtt
      181 tgggagggat ttttgccgtt tctcctcatt tccggactgg tcggattcct gctcgtcatc
      241 cagccggcga caagcattgt tttgatttta atggcggcga ctctgatcgt ttatttcgtg
      301 agcggcgcca aactttcttt tatcgcggcc atcaccatgc tcgctcttat cgccttcggg
      361 ctcttgatcg ctttcactcc ttacagactc gcgcgattcg tcactttttt taacaaagac
      421 gccgatcctc tgggagcctc gttccaggtg aaccaggcga tgatcgccat cggctccgga
      481 cggatcttcg gagtcggcta cggcaactcg acctcgaaaa tcatcggctt gcccgaaccg
      541 atcagcgatt ccattttcgc catcgccgcc gaggaattcg gattcgtcgg cgtcgttttc
      601 tttatcctgc ttttcttcgt cctaacactc gccggatttt tcgggagctt ggaaaccaaa
      661 accaaattcg ggaaattcgc catgatcggt ttttcctcga tcatcggcat ccaggctttt
      721 gtcaacatgg ccgccatttc ggggatgatt cctttgaccg gaactccgct gccgtttata
      781 agttatggag gaacgagcct cacggttttc atgaccatgc tcggattgat ggttaatttt
      841 tatcgccatg cgtaaattga gaatcgcctt tgccggagga ggaaccggcg gacacgttta
      901 tccgctcctt gccgtggcca cggaaacgca aatcctcgcc gcgaacaaac gtctggaaat
      961 cgagatgcgt tatttcgggt ctccgaagca ataccgaacg actctcgagg aaaacggtat
     1021 ttccgtcagt tcgatcatgg agagcaagat cagacggtat ttcgacttga ggaacctgat
     1081 cgatatcccg aaattcggac tcagcctgc
//
LOCUS       DAOPQT010000159         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_144660_length_1089_cov_2.403288, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000159 DAOPQT010000000
VERSION     DAOPQT010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144660_length_1089_cov_2.403288"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1089
                     /locus_tag="PKG74_03380"
     CDS             <1..>1089
                     /locus_tag="PKG74_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90342.1"
                     /translation="LEVFKINFRVKGVGASSIVVSDGAVTASDGSGTNVLVGTRGIDI
                     TVAAGQTLPSGQISVLPPIEQITRPATPAASLPDMPEVLVDLYPDPMVWHNASSPFLG
                     RWALPEDITDVATLVNKNPSSNPTVSEGLFDNKTFAALDDGIWYLHVRFRNNVGWGPT
                     DHYRIAVDTVPPSPFGILSSSGTSSTNPSPTISFFASDALSGIAGYSVQIDNGDYDSV
                     SGSSFTFPPLVPGVHDIRVAARDSAGNSTETFSELEIIPIESPTIDYLTPRVYIGEGG
                     LEAGGSSLPGYSVVVSLKNIEEAEIASTVVLTDESGNWFVQFNDPLRRGRYYIEATAK
                     DSRGALSLPVSSEEIRASSKPIIVVGGIG"
BASE COUNT          227 a          310 c          286 g          266 t
ORIGIN      
        1 tctcgaagtc ttcaaaataa atttccgcgt taaaggcgtc ggcgcctcgt ccattgtcgt
       61 tagcgacggc gccgtcaccg cttccgacgg aagcggcacc aacgtgcttg tgggcacgag
      121 gggtatagac atcacggtcg cagccggaca aacattgccg tccggacaaa tatccgtttt
      181 gcctccgatc gaacaaatca cccgtccggc aacgccggcg gcctcgctcc cggatatgcc
      241 ggaagtcctt gttgatcttt atccggaccc gatggtttgg cataacgctt cctctccgtt
      301 tctcggacgt tgggctttgc ccgaggatat caccgacgtc gcgaccctgg tcaacaagaa
      361 tccttcttcc aatccgacgg tttccgaagg tcttttcgac aacaagactt tcgccgcttt
      421 ggacgacgga atatggtatc tccacgttcg gttcagaaac aacgtcggct ggggcccgac
      481 tgaccattat cgcattgccg tggacactgt tccgccgtcg cctttcggca ttttgtcttc
      541 gagcggaaca tcttccacca atccgtcgcc gacgatttct tttttcgcga gcgacgccct
      601 ttccggcatt gccggataca gcgtccaaat cgacaatgga gattatgatt ccgtttccgg
      661 aagctctttc acctttccgc cgcttgttcc gggagtccac gatatccggg tggcggcgag
      721 agacagtgcc ggcaattcca cggaaacttt cagcgaactg gagatcatac cgatagaatc
      781 tccgaccatc gattatctga ccccaagggt ctatatcggc gaaggcgggc ttgaggcagg
      841 cgggtccagc ttgcccgggt attcggtcgt cgtttccttg aaaaacatcg aagaagcgga
      901 aatcgcttcg accgttgttt tgaccgatga atcagggaat tggtttgtcc aattcaacga
      961 tccgctccgc agaggaaggt attatatcga agcgacggcc aaagattctc ggggagcttt
     1021 gagcttgccg gtttcttccg aagagatcag ggcttcttcc aagccaatca ttgttgtcgg
     1081 cggaatcgg
//
LOCUS       DAOPQT010000160         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_145986_length_1082_cov_2.933788, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000160 DAOPQT010000000
VERSION     DAOPQT010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145986_length_1082_cov_2.933788"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKG74_03385"
     CDS             <1..287
                     /locus_tag="PKG74_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90343.1"
                     /translation="FFLTAMGLSILSEFVGDEGTMASFALAWIVLGFYAWLLYFEIMN
                     SRRLAWNRNKWGSFKDFQDSENRWTPWGYLMFAIIILTFAGSFIQGFLEV"
     gene            complement(338..949)
                     /locus_tag="PKG74_03390"
     CDS             complement(338..949)
                     /locus_tag="PKG74_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90344.1"
                     /translation="MVNMLKKQALFWLKISGLVMVIAAVFLFFYASEKKIEFPPELLE
                     AKTASSTIAEEITSLSGEAAANLEAISREDKAGNYYEAVDLVITEIKRNNEIREKCVG
                     LLSELQKMIAGLGEIDSVPAQAAGLQAISAEMTLVNQLMSYNDGLQTLLEHLRLKFLS
                     NSPKDFDAGTASILEEVNAEVEQINSQNRRAQTLIREFNKYFQ"
BASE COUNT          254 a          242 c          280 g          306 t
ORIGIN      
        1 acttttttct gaccgctatg gggttaagca tacttagcga gttcgtgggc gacgagggga
       61 ccatggcgtc ttttgctttg gcttggatcg ttttgggatt ttacgcgtgg cttttgtatt
      121 ttgagatcat gaacagccgc cgattggctt ggaacaggaa caagtgggga agctttaagg
      181 atttccagga ctccgagaat cgctggacac cttggggtta tcttatgttc gcgatcatta
      241 ttttgacttt cgcgggcagt tttatccagg gatttctcga agtatagttg ataataaata
      301 aaaacgcccc agaaagggcg tttttagcgt aaggccgcta ttggaaatac ttgttgaact
      361 cccggatcaa agtctgggct cttcggttct ggctgttgat ttgctccacc tcggcattga
      421 cctcctcgag aatggaggcg gttcccgcat caaaatcctt cggagaattg cttaggaatt
      481 tcaaacgtag atgctccaaa agagtctgta agccgtcgtt gtagctcatc agttggttta
      541 cgagagtcat ttccgcggaa atggcctgca agccggcggc ctgggccggg accgaatcga
      601 tctcgccgag accggcgatc attttctgaa gctcggaaag aagtccaacg catttttctc
      661 ttatctcgtt gttcctcttg atttccgtaa tgacgagatc aacggcctcg taataattcc
      721 cggctttgtc ttcacggctg atcgcctcca ggttggccgc ggcttcgccg gaaaggctgg
      781 tgatctcttc ggcgatggtt gaactcgccg tcttggcttc gagaagctcc ggagggaatt
      841 ctatcttttt ttccgaagcg tagaaaaaca aaaaaacagc ggcgataacc atgaccagtc
      901 cggagatttt gagccagaaa agcgcctgtt tcttcagcat attcaccata tattatggga
      961 tttctttaca ataatcaaat cttcgactaa cctgaaataa gtcgcgaaaa tgttccgcga
     1021 tcaaggtaaa agcgggatta gtatagtggt agtacgctac cttgccaagg taatggcgcc
     1081 gg
//
LOCUS       DAOPQT010000161         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_148441_length_1070_cov_2.854187, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000161 DAOPQT010000000
VERSION     DAOPQT010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148441_length_1070_cov_2.854187"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..943)
                     /locus_tag="PKG74_03395"
     CDS             complement(5..943)
                     /locus_tag="PKG74_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90345.1"
                     /translation="MIGPKIWTEGDEILATDLNKTGIGLRALEKSTPDMQLAVEPGVV
                     SISGTIVKYAGGNSGSFSAPSSNPRIDLLCIDSAGALSIVQGTEAASPSAPTYPAGKY
                     VIAEVYLRVGATSIKNTDDSTNGYIYRDVRAFFTGAGDQMVETFIAAETIAADDAVAI
                     GDYQTDGGVLMDTHKTTSGQANGSGALNFSGPISVGNNANRILLVFYYQQGGSGQGSW
                     SWNGSSMTSIIDTTMPSSGGRVGCLYLLAPSTGSQTLAITGLSANVGVRIWIYSYYNA
                     KQSGQPDNSNSKGGTTSTSAAITPNTSVSARRASQR"
BASE COUNT          209 a          285 c          327 g          249 t
ORIGIN      
        1 agctctaccg ctgggatgcc ctgcgggcgg acacggaagt gttcggcgtg atcgccgcgg
       61 aggtcgaggt cgtgcctcct ttcgaattgc tgttgtccgg ttgcccgctt tgcttcgcat
      121 tatagtacga atagatccat atacggacgc ctacgtttgc ggagagtccg gtgatcgcga
      181 gagtttgaga tccggtcgat ggagcgagga gatagagaca cccgacgcgg ccgccgctgg
      241 acggcatcgt cgtatcgatg atgctcgtca ttgaagatcc gttccacgac cacgatcctt
      301 gaccggaccc gccttgttgg tagtagaaga ccaaaagaat tcggttcgca ttgttgccga
      361 cactgatcgg accggagaag ttgagggcgc ccgatccgtt cgcttgcccg gaagtcgtct
      421 tgtgcgtatc catcaaaacg ccgccgtcgg tctggtagtc tccgatcgcg accgcatcat
      481 ccgcggcgat cgtctccgcg gcgatgaagg tctccaccat ttgatcgccg gcgccggtga
      541 aaaaggcgcg gacatcgcga tagatatagc cgttcgtcga gtcgtccgtg tttttgatcg
      601 aggtcgcgcc gacgcggagg tacacttcgg cgatcacata tttcccggcc gggtaggtcg
      661 gcgccgaagg cgacgccgct tccgtgcctt gcacgatcga gagggcgccc gcggaatcga
      721 tgcacaaaag atcaatgcgg ggattcgacg acggagcgga gaaggatccg gaattgccgc
      781 cggcatattt cacgatcgtc ccggagatcg agacgacgcc cggctcgacg gccagttgca
      841 tatccggagt gcttttttcg agagcgcgaa gcccgattcc ggtcttgttg agatcggtcg
      901 cgagaatctc atctccttcc gtccaaattt ttggtcctat catagtgttt tattttagat
      961 attttgagga tccggagaag cgtccggaaa ttcgagaagg tgaatttgat cgtgatctcc
     1021 gttttcatgt tagtcattcg agaaggtgtc ctcgtagtcg atcgtgagcg
//
LOCUS       DAOPQT010000162         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_152089_length_1053_cov_1.685371, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000162 DAOPQT010000000
VERSION     DAOPQT010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152089_length_1053_cov_1.685371"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="PKG74_03400"
     CDS             <1..805
                     /locus_tag="PKG74_03400"
                     /inference="COORDINATES: protein motif:HMM:NF020383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90346.1"
                     /translation="TSNDCPIGTKFFEFFNGSEEDKKLASKIGLGRRRNYYCNIVVLK
                     NDNHPEEVGKVFKWRFGQQIQQKINDKINPIDSSDPVMVHNPFDIVPLKLKAIEKNGF
                     RNFDTSEWILSGKSVADYVMPKASSDEKTKYMEALIEHLFHVSDFVRDDMFKSYDELV
                     DILNDVLLNHGLSPVSKEDGGVESKTVRNNTGTSFPNFSVSSKKNDDVKKESKEEVKE
                     ETKEEEEERDPFSDEEPEDEKESTSSIVDDDLDLDKIFGSSDNDDDAPF"
     gene            849..>1053
                     /locus_tag="PKG74_03405"
     CDS             849..>1053
                     /locus_tag="PKG74_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB90347.1"
                     /translation="MGKKEETVWDLTPDQFRILTNASRLNPIVYISKDFGGLITADQM
                     DLALDTFYMNKKFDYKLEQSFVIH"
BASE COUNT          362 a          221 c          235 g          235 t
ORIGIN      
        1 gacgtcgaat gattgtccga taggcaccaa gttctttgaa ttcttcaacg gttctgaaga
       61 agacaaaaaa ctggcgagca agatcgggct tggacgaagg agaaattatt actgcaacat
      121 cgtcgttctc aagaacgaca atcatccgga agaagtagga aaggtcttca agtggcggtt
      181 cggtcaacag atacaacaga agatcaacga caaaataaat ccaatagaca gttctgatcc
      241 cgtcatggtc cacaatcctt tcgacatcgt cccgttaaaa ttaaaagcga tagagaaaaa
      301 cggtttcagg aacttcgata cgtctgaatg gattttaagc ggtaaatcgg tcgcagacta
      361 cgtcatgccg aaggcttcgt ccgacgagaa gacaaaatac atggaagcgt tgatcgaaca
      421 tctgtttcac gtcagcgact tcgtaagaga cgacatgttt aagtcgtacg acgaactggt
      481 cgacatcctc aacgacgttc ttttgaacca cggcttatct ccggtttcaa aagaagacgg
      541 aggagtcgag tcgaagaccg ttcgaaacaa caccggtact tcttttccta atttttctgt
      601 ttcttctaaa aagaatgacg atgtcaaaaa agaaagcaag gaagaagtca aggaagaaac
      661 caaggaagaa gaggaagaga gagatccatt ttcagacgaa gagccagaag acgaaaaaga
      721 atcgacgtct tcgatcgtcg acgacgacct tgatctcgac aagatctttg gttcaagcga
      781 taacgacgac gatgccccgt tttaaaaacc ttgaaataaa ataaaataaa tcagaggagg
      841 aaaaactcgt ggggaaaaaa gaagaaactg tctgggatct cactccagat caattcagga
      901 tactgacgaa tgcttcgaga ttaaatccga tcgtttacat cagcaaagac ttcggaggac
      961 tcataaccgc cgaccaaatg gacttggctc tcgacacgtt ttacatgaac aaaaagtttg
     1021 actacaagct tgaacagtct ttcgtcatcc aca
//
LOCUS       DAOPQT010000163         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_159766_length_1018_cov_2.801661, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000163 DAOPQT010000000
VERSION     DAOPQT010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159766_length_1018_cov_2.801661"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1018
                     /locus_tag="PKG74_03410"
     CDS             <1..>1018
                     /locus_tag="PKG74_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOB90348.1"
                     /translation="KSEDKEAARKNLVKNFDLSELQAEAILEMKLQSLAALEREKIEN
                     ELKEKKKLIAELEALLKSAKAILKVVKTEMLALREKFGDERKTKVVASGLKEFSDEDL
                     IPEEEVMITFSKSGYIKRLPPQNIKSQHRGGKGVIGSDVGEDDFLTHFISAATHDNIL
                     FFTEKGRVFQTKVYEIPAASRTAKGRLIQNFLEIPADEAVNAIVTYSNEQLKSGSGYL
                     VMVTLKGVIKKTSIKEFGNIRRNGIIAINLNKGDKLVGAKLSSGKDEIVITSIEGQAI
                     RFKESETRAMGRAASGVRGINLSSSDKVASFDIVPAEKKSANLLSVMDNGFAKQTPLK
                     QYKVQ"
BASE COUNT          298 a          234 c          279 g          207 t
ORIGIN      
        1 gaaatctgaa gacaaggaag cggccagaaa aaacctggtg aagaatttcg atttgagcga
       61 gcttcaggcc gaagccattt tggaaatgaa gctccagagc ttggccgctt tggaaaggga
      121 gaagatagaa aacgaactaa aggagaaaaa gaaactgatc gccgaattgg aagcgcttct
      181 gaaaagcgcc aaggccatct tgaaagtggt caaaacggaa atgctcgcct tgagggaaaa
      241 attcggcgac gagagaaaga ccaaagtggt cgcctctggg ttgaaagagt tttccgacga
      301 ggatcttatt cccgaagaag aggtgatgat cactttctcg aagagcggct atatcaagcg
      361 gctgccgccg caaaacatca agagccagca tcggggtgga aaaggggtta tcggttcgga
      421 cgtcggcgaa gacgatttcc tgacgcattt catttccgcc gccacccacg acaacatcct
      481 tttcttcacg gaaaaaggcc gcgttttcca aaccaaagtt tacgagattc cggccgcgag
      541 ccgcacggcc aaaggccggt tgatccagaa tttcctggag attccggcgg acgaggcggt
      601 gaacgctatt gtcacctatt cgaacgaaca attgaaatcc ggttccggct atttggtgat
      661 ggtgaccttg aaaggcgtca ttaaaaagac gagcatcaag gaattcggga acattaggag
      721 aaacggcatc atcgccatca atttgaacaa aggagacaag ctcgtcggag ccaagctttc
      781 ttcgggcaag gacgagatcg tcatcacttc gattgaagga caggctatca gattcaagga
      841 atcggaaacc agggccatgg gccgggccgc ttccggagtg cggggcatca acctttcttc
      901 ttcggacaag gtggcttctt tcgacattgt tccggccgag aagaaatcag ccaatctttt
      961 gagcgtcatg gacaacggct ttgccaagca aactccattg aagcagtaca aagttcag
//
LOCUS       DAOPQT010000164         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Colwelliibacteriota bacterium isolate
            AMR_MDS_1515 NODE_162953_length_1005_cov_1.405263, whole genome
            shotgun sequence.
ACCESSION   DAOPQT010000164 DAOPQT010000000
VERSION     DAOPQT010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854720
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Colwelliibacteriota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Colwelliibacteriota bacterium
            Bacteria; Candidatus Colwelliibacteriota.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:12:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 683
            CDSs (total)                      :: 650
            Genes (coding)                    :: 647
            CDSs (with protein)               :: 647
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Candidatus Colwelliibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162953_length_1005_cov_1.405263"
                     /isolate="AMR_MDS_1515"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2750074"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..>1005
                     /locus_tag="PKG74_03415"
     CDS             177..>1005
                     /locus_tag="PKG74_03415"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HOB90349.1"
                     /translation="MPPTNGVRGTDNLGVTRKYDVADVISLLDVARYPLLAILTNAGK
                     DPVTKEGNALKKKETTDPEFKWFEDKFGAREDIVAANQSGATSLVVTNGSYFSVGDVL
                     LCVKSGQATNNPTGEVMLVTNVNTNTLTVQRQVGDGSLGGGLLQQNDIVWIIGNANEE
                     GADIREIKATAVAEAINYCQIFRTTIGITETAKNTKGWTKESDFDYQTRKKGIEHLVD
                     VERAFMFGKKELIMSGVHPKRFTAGIFQRINQVVSNVATRDDFNGFLRTLFAHGNTEK
                     "
BASE COUNT          301 a          227 c          269 g          208 t
ORIGIN      
        1 aagtcgagag aggagcgggt gctccgacct cgcacggcga agacaagggc gcggaagaac
       61 gccgcctcga acaaatcttc ggaacaagcg ggtcaaaaag tcccttggga ggtctgggag
      121 tataactcca tcaaggtcga gcctcctata attaaccaat ttaacgataa aatcatatgc
      181 ctccaacaaa cggagtaaga ggaacagaca atttgggagt caccagaaaa tacgatgtgg
      241 ccgatgtcat ttcgcttctg gatgtcgccc gctatccgct tctggccatt cttaccaatg
      301 ccggaaaaga tccggttacc aaagaaggta acgcgctcaa gaaaaaagaa acgactgatc
      361 cggaattcaa atggttcgaa gacaagttcg gcgcgcggga agatatcgtg gcggccaacc
      421 agagcggcgc gacatcgctg gtcgttacca acggttcgta tttcagcgtc ggcgatgttt
      481 tgctttgcgt aaagtcaggt caggcgacga acaatccaac cggagaagtt atgctggtaa
      541 cgaacgtaaa caccaacact ttgaccgttc agcgtcaggt gggtgacgga tcattgggtg
      601 gtggactcct tcagcaaaac gatattgtct ggataatcgg aaacgccaat gaagaaggcg
      661 ctgacattcg agaaataaaa gcgacggcgg tggcggaagc catcaactat tgccagatat
      721 tccgaacgac tatcggtatc acggaaacag ccaagaacac caagggatgg accaaagaaa
      781 gcgatttcga ttatcagacc agaaagaaag gcatcgagca tttggtcgat gtcgagcgag
      841 cttttatgtt cggcaagaaa gaattgatta tgagtggtgt tcatccgaaa cgatttacgg
      901 ccggaatttt tcagagaatc aatcaggtcg tttccaacgt ggcgacgcgc gacgatttca
      961 acggattttt gcgaaccttg ttcgctcacg gcaatacgga aaaat
//
