LOCUS       DAOPRN010000001        74596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_252_length_74596_cov_118.887914, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000001 DAOPRN010000000
VERSION     DAOPRN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 74596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 74596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74596
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_252_length_74596_cov_118.887914"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1466)
                     /locus_tag="PKI65_00005"
     CDS             complement(207..1466)
                     /locus_tag="PKI65_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOB76861.1"
                     /translation="MSIPLAEQLRPKNWDEFVGQEHLVGADGIIRKLLSSKSLPSLIL
                     WGSPGTGKTTIAGLIAKDLQVPFFKLNAIDAGVKDIRNIIQKAQPYKTVILFLDEIHR
                     FSKSQQDSLLHAVEKGIITLIGATTENPSFEIIPPLLSRCQVYVLNPLEISDLDKLLQ
                     RAIDFYSSKNIIIEVIEKEALFTYSGGDARKLYNVIELLVDSSNSTNLVISNENVEAI
                     VKQKFLLYDKQGEYHYDLISAFIKSIRNSDPNAAIYWMARMLEGGEDPLFIARRMIIL
                     ASEDIGLANPNALLLANACFDAVHKIGMPEARIVLAECAIYLASSPKSNASYLSIEKA
                     IEFVHKTGDLPVPLHLRNAPTQFMRSLNYHKGYLYPHDHPNHFIEQECLPDEISGTSF
                     YIPADNSKENELKNYLKKLWKNKYRFE"
     gene            complement(1500..2750)
                     /locus_tag="PKI65_00010"
     CDS             complement(1500..2750)
                     /locus_tag="PKI65_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOB76862.1"
                     /translation="MNIAILSIGDEILRGIIDNKNASYIANKLFMNGFNTKTIISISD
                     NLDEIVETLNQLSKSHEIIISTGGLGPTPDDKTIEAIAKWSEVPLVENNKAKQLIKNH
                     YKKRNVQITQMRKKMFLIPETSELLPNSVGAAPGIKITKGNTTLFALPGVPQEAQAVL
                     DEAVLPWIIQNYSPVIWTTALTFRNIRESELATYIESKINDFDLLTVAYLPSFGYVRI
                     LFSANKDYTNDNSFNQFLNDLIFKYQEFFVYKSIEKPEQYLHKLMLNTGTTLCTIESC
                     TGGIVASKIVSNAGSSEYFRGSLICYSNEIKEKILKVPKDILTTKGAVSEETVKYMAE
                     NGKNLFNTDYSIAISGIAGPTGATPNKPVGLVHFAISTPSNIYCYKFVFHGNREINIE
                     SAANTALLLLIQHIEFFKINFIKN"
     gene            3103..4227
                     /gene="tgt"
                     /locus_tag="PKI65_00015"
     CDS             3103..4227
                     /gene="tgt"
                     /locus_tag="PKI65_00015"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOB76863.1"
                     /translation="MNFEIISQCGNARAGILRLPHGNVQTPVFMPIGTVGSVKGIMWD
                     DLKNILKVQIILANTYHLYLRPGIDILYEAGGLHKFINWQRPILSDSGGYQVYSLANI
                     RKINDDGVEFRSHIDGSKHIFTPEKVMKIQQYIGSDIIMAFDECTPYPVDYEYAKNSL
                     NLTHKWLERCIDYLEHNPPLYGYMQFLFPIVQGSIYSDLREASALFVSKFDTLGYAIG
                     GLSVGEPADIMYEMIKIVNNILPHNKPRYLMGVGTPANILQAVALGVDMFDCVIPTRN
                     GRNGQLFTSQGIINIKNKKWSRDFSPIDLNSPLELDQTTTKAYLRHLFLAGEMLGPMI
                     ASYHNLAFYIRLMNEIRYHILEGSYDQYYIKAMDTYMKRL"
     gene            4224..5306
                     /locus_tag="PKI65_00020"
     CDS             4224..5306
                     /locus_tag="PKI65_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOB76864.1"
                     /translation="MKIKKLDLYIAKKFISSFILIITLLIFIIIAFDISEKIDKFIAK
                     NAPVSLIITEYYFNFIPYFINLFSSLFIFISVIYFTSKLASRSEIIAMYSVGIDFKRI
                     LRPYLVVSFCLAIFSFVLQNFILPPANQRRINFENVYIKRNPQTKESYIHMQLDKNNF
                     VYMHTWDVKDYEGRFLSMDKIDYQKGVTNKMIASFSHYDTISKKWQLTDVIIRNIKDS
                     IEQLTRYKNLDTILPITPSDLNYETTLLETMNFTELNKYIRKQKERGAENINELLVER
                     QNRYASPFSAIILTIIAVTLSINKKQGGLGLNLAIGLSLSFGYILFQQISVSYGVAGI
                     LSPFIAAWLPNFIFIIIAIYLIYREI"
     gene            complement(5657..8017)
                     /locus_tag="PKI65_00025"
     CDS             complement(5657..8017)
                     /locus_tag="PKI65_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB76865.1"
                     /translation="MKKIFFITYLIIIFCFLYINSLKAQLIVNIIPNDTSICRGDSLI
                     LNATSNLPFTTFQWSTGDNTSSITVAPVSTTTYAVTGTSSSDETASASVTIIVKDNPN
                     LTYHLTTSVFCDVGGIDTITINSDMPGTEYIWSTGETSDQIIVSIDSTSVFFVTGTAP
                     NGCSATASIFVMLAPNPTIITTNDTICLGETGYISVSGDAMGYEWNNGSNQFTISDNP
                     TITTTYTVTGISFLGCTASATATIVVNPLPTITIQATDQNVCQGSETTLIASGGMNYV
                     WSNGETTNIINSIINQQTSFSVTGTDNNGCKNSATIQINVYPLPTIIVNDRTICIGDT
                     VTLYAQGASTYIWSNQQSGNSIVVSPSTTTTYSVTGTDNNGCTGSTIAVVTVLSNPVL
                     IVNDTAICEGESATLTVSGADTYHWSNGLNTPSIFVTPNQTTTYIVTGYLGSSCSASA
                     SITVTINPLPNFEINTQPAYCDAFNGQATIEHLSGTPPFQYLWNTYPQQTTQEITELC
                     GGTYFVTVVDANGCKTTGQTFIETLPPFNITSNVLPEHCGNKDGSIIINVNGNLHPLT
                     YYWSHNPNLDTNELYNLSSGYYHVTITDDLCTKDTSIRLPYWHGPNARFTPSTYLWYM
                     DGEPIVFYNYTVGAISYLFEFGDGTSSTDINPSHRYNESGTFVITLYATDEYNCTSIC
                     VDSITVLENFRIFIPNAFTPNNDFLNDYFTPFIQGIDIDSYEIFIFSRTGKLLYHSKD
                     INKPWDGNIVDKNERNINPNTFLYVIKLRTINEKDKIYRGVINLIH"
     gene            complement(8021..10771)
                     /locus_tag="PKI65_00030"
     CDS             complement(8021..10771)
                     /locus_tag="PKI65_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB76866.1"
                     /translation="MKKAFIIFFIFLPLFIYNQTYTVHHPIPWALNHQYMWGPQGSSW
                     NLNLNQDIFHFNINENQSFGYIEDLGTLGQWGIDLNLNAYLDIALSYYASGFHNGWVD
                     INYPVDITLTFPDYGTIIPGQWATISSDYNVLPNAYLHPTFPDEGVIGMDFNFSANIN
                     LGGQLCAFNCTPYNVFNINVPYDSVILLELNTYTGIATYPCISGGYPDICYSNVIPLE
                     VNDIGGIGLALWADIPYISSYEDTVLSNKCLKASGVDYYMNLTLNLIQFIESMADIIG
                     GSTGDAIYNFINSLSGSIQIYNDPDMTIELNYTLIDQANLFTKHYLNQDLTFCPTIYT
                     TFHFPTPVEYKEIDTNNNVIQSGINDTVTFIVGNNLNILWPCFGLDSLSTGPILHFMK
                     NQFNNHVWDSIDLNFNIIGLSFTLNLDINVSKEAMVNVPPLCVEINANEIVNIENNIN
                     NLKDAKSHYNFHFGPFLDDTLHITDFALTWMNDTWEILGFNDSIFQAELLSSAPSIGL
                     NIIGDTILCQGMQNGIIYAQVQNGSPPYNFLWSTGNNNITNTPIDSIIVGEGTYSVTI
                     TDAGGCSVSDSINIFSNPPINISLDKIDVWCKHDSTGTIMIIASGGTPPYSYVLHPNN
                     ISSTTNIFDSLPKGSYIVEIIDSYGCVAYDTIYLVELYELPPVNIMAEPTIGCQPLYV
                     QFYETNYEYFPTNRYLWSFGDTIYNPLHKFNTAGNIDIWVQVTNEFGCDTTAYYSQFI
                     TVYPSPQANMIIDPVLVYATDDPTFTIQCYNQSLGDTYISWDFGDSTTSTDDFPSHSY
                     QNDGTYTVTLIAYNEYGCSDTISRAVLMIDDVLQIPNVITPNGDGINDAFIIENIDKY
                     PYSTLTILNRWGNVVYEINGYDNTWDAKDQPGGTYYYILYTGSGPNNPDKVYNGTITI
                     IK"
     gene            complement(10790..11269)
                     /locus_tag="PKI65_00035"
     CDS             complement(10790..11269)
                     /locus_tag="PKI65_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76867.1"
                     /translation="MKNNFILTILLLFLSQVLIGQTIIYYPPKGIVETKYSPTDNIYN
                     YQDTLGIISIQIKLISNRNFYNFKETFNQEKFFFDNQLIPMLKNFDFTISNKTGILLK
                     GYIEVLHNEQIIEIVRTTFFTGDSNNIVIFTAAYPKEIDEIFYSPLLESLRSLELKN"
     gene            11416..12000
                     /locus_tag="PKI65_00040"
     CDS             11416..12000
                     /locus_tag="PKI65_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOB76868.1"
                     /translation="MKEYIDMSDEELVIAYKQGDQRSFQELYKRYQRRIFSYILVSVK
                     NKEIANDIFQDVFFKVINTIKSGNYREEGKFAQWIIRITHNAVVDHFRYQNKYAISNN
                     NCDDNENYFFDSITKDHSISPEELLIKKENKYLVRQAVSTLSDKQKQVVYLRMNEDKC
                     FREIAEAQNISINTALGRMRYAIMNIKKKIDPFL"
     gene            complement(12232..13152)
                     /locus_tag="PKI65_00045"
     CDS             complement(12232..13152)
                     /locus_tag="PKI65_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOB76869.1"
                     /translation="MNKTKKILIAVIFIASIGFLYWGINFLKGLKIFSDNTIVYVDYD
                     ETYGLTTNSPVLLNGLNIGKVDEFKFISDDYKVRVRLLIQTNIEIPKNSIALITSGDF
                     LEGKVIKIILGDAKEYIKDNDFLKGEVSPDFTQILGAELSPLKEKAITLMASIDSTIS
                     IFQNIANPQVQNDLIMTIENIKKTTISLNKSLENIENISSNIKANNDKINQIITNINQ
                     ITDTIKNSDLHLVINNLNKDIYELQKIIDTINMKSGTIGKLIYDDSLYFYLTKSSQSL
                     NYLLEDLRKNPKRYINLSLISFGSSSKERK"
     gene            complement(13214..14221)
                     /locus_tag="PKI65_00050"
     CDS             complement(13214..14221)
                     /locus_tag="PKI65_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76870.1"
                     /translation="MKQLCLFINILIFNFLLGQKSTLVYPPHLDSLLLYENLIIEGKN
                     DFIKLQSNEYFFRLISEILSYPDAYRYDFDTLKILYTLRGDDFLIITWGVSLSTGKTI
                     PYGFTLKKLSKDDTYRLTELKNIKDSLSDPERAILKNGDWWGAIYYQIIPLEINNIKC
                     YTLLGWDGENELYQSKLIDVLSFSYNGLPIFGKRIFRNIPDKKNPTRLIFRYSDEAVM
                     TLRYEKQSYEINTVKIKKRNKPNRSRNFKKAQKRDKKIVKTKRYNRNMIVFNTLSPLN
                     KFLDEQYQYYFPNAEDLVGLELIDNRWVYRTNIDARNAPDDQDNYNPEKRIHKNKTPK
                     Y"
     gene            complement(14278..14820)
                     /gene="rfbC"
                     /locus_tag="PKI65_00055"
     CDS             complement(14278..14820)
                     /gene="rfbC"
                     /locus_tag="PKI65_00055"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009039543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOB76871.1"
                     /translation="MEAINLEIEGLILFKPVIYKDNRGYFLETQRKSHLNEAEITDDF
                     VQENESLSIRNVIRGLHFQVPPHPQAKLVRVVKGKAIDVAVDLRKNSPTYGKYLMIEL
                     NETEKQSFYIPIGFAHGFISLADETILLYKCSDYYSKECEKTIAWDDPTLNIPWNCKA
                     PILSDKDRNGILFDDFNSPF"
     gene            complement(14826..17207)
                     /locus_tag="PKI65_00060"
     CDS             complement(14826..17207)
                     /locus_tag="PKI65_00060"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOB76872.1"
                     /translation="MDEKPKIPKYNNEFNPYIFKIVFKKNLWLLLLYLFVTLFIVFAS
                     IRYTKPVYQAKAVIQLNIENRASEVISSSALNKDNIYTSVEVLSSPEFIKRVIKQLPL
                     NTSIYAKGKILDFELYRSAPFLFTYEIIDSLIINKLINIEIKNDNTIDIFYKINNNTY
                     ATQGTFYDTIILDHIKFVLIPKDEIKEGDYCIIIHDLPSLIKQYRYQLSTNILNPNAG
                     SIELSFSDQNPLKCVDFLNTVIQEYKFFDVEQRTMSARSMLEYIDQQLESLEDIIKQQ
                     SGNDNIALSTNDTFTSFFSLKQEINKLELEIERINDEIVILNKSQEILNKDSLNTSRL
                     IVYLSQTSYYNILSTFVNEYRSLLEQYLALSAIYSTNTYTLEQLKWKIHYSKNSLNDI
                     LETLIERSNFQLSSLKKQLQNYKSKLSNIEKKAGITTMEPLTDISQSSLKYYEQFLNK
                     KLEYTLYVAGIVSDLVVLEEPTLLSNPIYPNKTKSLIIGLFIFILISTSTLIWKYIKY
                     DNILSIQDIESLFPDTPILSLPLFNGDIPDSQLIIHKYKRSLLTESFRKIKSHLLLIN
                     QDNIPKVIVITSTIPGEGKTFIAINLGGILALSNKKVLIIDTDLRRPKMSIGFNNKSE
                     LGLSSYLQGECSIEQCICHTELSNLFYFPAGPLPEYPNELLTSNKMDKLIEELTQSYD
                     IVILDTPPIGLISDATKFIMLADTTFYVFRSDFSKKEFLYNVKKLKQELNPKNLILVL
                     NGFNEKYIRKAKYSNYGYEYKYDIYGYYDGYGVKETKQSKSMKKYLNYFSKKK"
     gene            17308..18060
                     /locus_tag="PKI65_00065"
     CDS             17308..18060
                     /locus_tag="PKI65_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOB76873.1"
                     /translation="MHKIFFVHNQAIVIIPTYNEKENIESIIRTVFSLPEPFDILVVD
                     DNSPDGTSDIVKSLIDEFPDNLNLLLREKKEGLGRAYIAGFKWALEHNYEYILEMDAD
                     FSHNPEDLVRLLATCRDDNYDLSIGSRYVSGITVMNWPLSRVLLSYFASKYVNIITGM
                     PVKDSTAGFVCYKASFLSSIDLDKISFIGYAFQIEMKYYAYKLGFKIKEIPIIFSERQ
                     HGQSKMNKKIVFEAMWGVIQMRFRNVKKLLKK"
     gene            18060..19391
                     /locus_tag="PKI65_00070"
     CDS             18060..19391
                     /locus_tag="PKI65_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOB76874.1"
                     /translation="MDRFHIYNVNIINEGKIFNGEIFISNGLIEKINHNSSSSTPTSY
                     QKIDGQGNYLIPGIIDSHVHFREPGYIDKATIASESLAALYGGVTSFIDMPNTLPPTT
                     NKKTLEHKLIIARDKSFINYGFFLTAVENNILDLQQINPTQIAGIKIFWGDTTGGLNL
                     IDTEIINEVFKLPFLIAVHSEDNATIQYNESLYLGSFKNDVYIHPIIRGLDACIIAAK
                     DILGLASKYQTRLHYLHVSHINELNFILQWRDQIKHLSIETCPHYLYFDLSHYDVFGN
                     LIKVNPSIKSTPEQRIQLLNSLNIIDTIASDHAPHLKHEKQKNYDLAPSGLPSIQHNL
                     GLMWNLVNQGYLSLPDLIEKMTHNPAKIFKIKNRGYIREGYYADLVLVDDKVKETIST
                     EKLKYKCGWSPYENFTLPISIKKVWVNGNLILDDGKLNDIKSAQALIFENY"
     gene            19510..22140
                     /locus_tag="PKI65_00075"
     CDS             19510..22140
                     /locus_tag="PKI65_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOB76875.1"
                     /translation="MKYFNFSLMFFFLINASIFSQNYFYEDFSDGDFVSNPEWTGDVD
                     KYIIYTGTAVPANMKPSLRTNGSATDTTYLATEFVQSFTDSLEWNFWVKLSFNPSSGN
                     YARIYLCSDNENLKGSLNGYYVAIGYNGNDRVTLVKQQGTNHTALIVGSSADLNKSTN
                     ILRIRVKRYDDGTWLMYSDESGGSNYTLEGQFTDATFTSSNYLGVFNQYTSSNATSFY
                     FDEFYAGPIIIDTIPPKVISAKVINSFDLAITFSETMDNSIYDSDNYYADHGLGIPTS
                     VLDDPNSSNTVHLSWMPNPIIDDTIYHLSINNVSDLAGNTLDTTIIFTFHIPVAYDVL
                     INEIMADPDPPIALPPYEYIELYNPNHFPINLENWKIRISNTTRIIGNVDIDPNGYLI
                     LCHQDGMQDFENLPQHCKTFGFSSFSITNAGADIVLYSPDGNVISFAAFRDTWYQDPL
                     KAEGGYSVEMIDPNNPCADETNWRATNSLFGGTPGIINSIHGSNPDNDNPEILRAILQ
                     NSTTLLVLYTEPLDSIYALDSSNYIIEPLLTINNIKGNEPFYNEITIIFNESVDSSEI
                     YTIYFKDSIRDCVGNMLPHETSARFAFGNIPEPYDIVINEILHDPIGNNTPFVEIYNR
                     SNKILDLKYCVLANRDSLYELANKKSIAPGGFPIFPEEYIVLSKNKKQILSQYYTPNP
                     KNFIDMNLPKITKDSNTIVLALTNDTIIDLVKYSIKYHDPLLISTDGVSLERVNPNRP
                     SNESSNWHSAAATVGFATPAYKNSQYSDDLFTDEVTIDPPYFSPDNDGFQDLLDICFD
                     FKDPGYHVSITVHNLAGQPVRHLLRQEYVGTHMCYSWDGRDDNDKRCEFGWYIIRISA
                     FNTKGDKKEFKKAVSLNIRK"
     gene            complement(22295..23551)
                     /locus_tag="PKI65_00080"
     CDS             complement(22295..23551)
                     /locus_tag="PKI65_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76876.1"
                     /translation="MLNPNDKNILVAIPLMNEFENISTLMECLADQKLRAKVIALCVN
                     QPKHWSDSPDKKEIVCNNIKTLNYLNQISTSYPIKLIILDHASTGKGFASKIPSVGLA
                     RKLCIDALLPLANDDDIIVNIDADTYYPSNYLNSLYNIYKQLPLLQGICAPYYHKINN
                     LSSNQALLMLRYEIFLRYYTIQLLLIKSPYAYMPIGSAISFTVRAYKKSGRFKIRDAG
                     EDFYTMQKIRKIGMVSGWSDTNVYPSARSSDRVVFGTGPAINLTLREQSKKYPFYPTQ
                     LFEAIRDIYQSIEHFDMIDDIVNHLEKIIPREIITKIHNNNPKKHRFIHAIHEYFDAF
                     HIIKYLNKNYKVDPEKDFEHFKKFIKINFNKEIKYDKWEQIPIDELNTIRDQLYKFEL
                     NLQKSKYNYLINNQNNDKIWKYLSTW"
     gene            complement(23775..24935)
                     /locus_tag="PKI65_00085"
     CDS             complement(23775..24935)
                     /locus_tag="PKI65_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOB76877.1"
                     /translation="MSRILIVDDEKSVRETLREMLEYENYEVIDAENATKALDLLSSQ
                     SFDVILCDIKMPGMDGMELLEKILENYDIPVIMISGHGTIETAVDAIKKGAFDFIIKP
                     FDLNRLLISIRNALDRSNLMEETKRLKNKLSKKYALIGDSPAMKKLKENIERIAPTDA
                     RVLIMGENGTGKENIAHWLHELSPRAKGPFVEVNCAAIPSELIESELFGHEKGAFTSA
                     IKSKKGSFELANNGTLFLDEIGDMSLAAQAKVLRTLEENKITRVGGEKDIPVDVRVIA
                     ATNKNLVEEIKNKRFREDLFHRLAVIILEVPPLRERKEDIPLLIDHFVNELSESIKKP
                     TIKFDENAIEYLINLPWRGNVRELRNVVERLMILCNEKVSLEDVKNFANPYY"
     gene            complement(25080..26567)
                     /locus_tag="PKI65_00090"
     CDS             complement(25080..26567)
                     /locus_tag="PKI65_00090"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOB76878.1"
                     /translation="MNIKSFLKISTLSLVFIFIISACAQEKSRTTGWVYNDPNWGGFE
                     VAPYEEQETGPGLVLVTGGTFVMGRTEQDVTYQWDNVPRRVSVSSFYMDMAEVANVDY
                     REYIYWLSRVFGTDYPEVVNKALPDTLVWLSRLGYNDPFVEYYFRHPAYNFYPVVGVN
                     WRQANDYASWRTDRVNEYILVREGILKLDNMQMNENNFNTDAYLAGQYEGIVKNELYD
                     LNPTGMGTRKVRVEDGILLPKYRLPTEAEWEYAALGLIGNTVYERVLERRVYPWNGHV
                     LRTDYHKNMGDFVANFVRGRGDYMGVAGYLNDGYDATAPVFSYWPNDFGLYNMAGNVA
                     EWVLDVYRPLSYEDMDEWRPYRGNVFMTKVRDEEGLIAEKDSLGRIQWRPLTEEEAKN
                     RYNFRYADNRDYLDGDYTSSIYYDQADYLEEDQRNKIMYEYGKTSLINNQARVYKGGS
                     WKDRAYFLSPGTRRFLDEEMATDYIGFRCAMTRVGAPVNYGSKNR"
     gene            complement(26593..27576)
                     /locus_tag="PKI65_00095"
     CDS             complement(26593..27576)
                     /locus_tag="PKI65_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOB76879.1"
                     /translation="MKKKYTFILILLIGSITILKGQDPHFSQYYANPLYLNPALAGAQ
                     ICPRLAFNFRNQWPSITGTYVTYNASYDMYIPALSGGIGLLVNVDRAGEGILNTTMAG
                     FMYAFRLKVSDNFYINTALQATFVQKSLDWEKLIFPDQIDPRWGIIHNTNEIPPENLT
                     KNFADFSAGIAGYGDRYYFGFSASHVTTPNEGFTSTSYLPVRFTLHAGGIISIESKPY
                     KKLTIEETTLSPNILFSKQQDFEEFNYGLYLNKYPFVVGTWFRHSTKNPDAIIVLVGF
                     IQELFQIGYSYDITVSRLTNQSGGAHEFSLTFRLPCPVKAKKIAPINCPKF"
     gene            complement(27710..28624)
                     /locus_tag="PKI65_00100"
     CDS             complement(27710..28624)
                     /locus_tag="PKI65_00100"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOB76880.1"
                     /translation="MKKIFCIFLILLILHQNVSVNSQELNCNISINSSAITNVDQSIF
                     DNLQSSIYEFMNARKWTNYNFEPEERIECSIIITINSYDNVNMYGGTFQVQLRRPVYN
                     TSYHSTLFNFQDKDIQFQYTLGTSLDYIENTFVGNLESLLAYYAYLFIGLDFDSFQEL
                     GGNPFYEKAWSVINSIPANATQYKGWRSYENQRNRYWILENLTNKSYDNYRKGFYLYH
                     RHGLDIMVDDIVKGRSNIIQAMKLIQQAYKEKPGLFIINLFMTAKSDELVNIFKPAPT
                     MEKTEIVNILKSIDPMNASKYNEILVSQ"
     gene            complement(28637..29836)
                     /gene="coaBC"
                     /locus_tag="PKI65_00105"
     CDS             complement(28637..29836)
                     /gene="coaBC"
                     /locus_tag="PKI65_00105"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOB76881.1"
                     /translation="MYLQDKHILIGISGSIAAYKIPTLIRVLLQNNAEVRVVATKNAL
                     QFVTPTTIRTLTNYDVYTDMFDNAKFTGTQHISLTDWADIFLIAPATTNIIGKMAHGI
                     ADDALSTTFLSFDKQVFIAPAMNVKMWQHPILQQNLKILKNIGIKILNPDTGELACGY
                     KGTGRMMEPQQIVDEINAFLGSQSVLLGKKILVTAGPTYEPIDPVRFIGNKSSGKMGF
                     AIAETLAEMGAEVILIAGPTNLSISHPKVTRINVFTTNEMLDQCNKYFDHVDGVIMNA
                     AVSDYYPINIATEKIKKSDFLNLELKKTPDILLELSNKKNHQWIVGFALETENEEKNA
                     INKLKNKNLDAIILNSAKTTDIGMGSDFNKITIFTKNGKKLNTDKLPKKQIAKYIIDN
                     VIVDLCK"
     gene            complement(29847..30167)
                     /locus_tag="PKI65_00110"
     CDS             complement(29847..30167)
                     /locus_tag="PKI65_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOB76882.1"
                     /translation="MKKKDHRIWQEAVTRDIKQISEITGNIYLSTLIIGKRANQISQQ
                     TKEELDERLSEFKTTVDTLEEYIENKAQIEVAREYEMKPKPTLIATKEFLNKKIYFRI
                     PEHQ"
     gene            complement(30164..30976)
                     /gene="bamD"
                     /locus_tag="PKI65_00115"
     CDS             complement(30164..30976)
                     /gene="bamD"
                     /locus_tag="PKI65_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOB76883.1"
                     /translation="MKLKYSLFFILIITLTSCKENVEFNKIVKADDPELKYAKAMEYY
                     QQGSYYKAIQLIEMIIPYYRGTTKSQELYYHMAWSYYYQGDYIMASYHFKEFARTFPS
                     SKYAEECAFQTAYCKYMLAPPYYLDQSITKGAIKDFQLFITRYPNSVKVKDAKECIDK
                     LTANLEKKDFTNSKLYYDIKEYKAAIVSLNNFIKDYPASKFKEEAQFYLCLSHFYYAL
                     NSVADKQLPRFLEAIDVINNFTMLYPDSQYANKLNLIKAEIEKQIESKQNIL"
     gene            complement(31067..31429)
                     /locus_tag="PKI65_00120"
     CDS             complement(31067..31429)
                     /locus_tag="PKI65_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOB76884.1"
                     /translation="MKKLFLIVVLAFTIPVFVNAQVSVPSQNSPTKKAEFKVKTYFHC
                     ENGKKLIEDQLSKREDIYSVNADLETKIVTIVYNPERYKETDLIYLIHKVGYLTEKSP
                     KDTKIEKACSHDHEGDKH"
     gene            31660..32391
                     /locus_tag="PKI65_00125"
     CDS             31660..32391
                     /locus_tag="PKI65_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOB76885.1"
                     /translation="MDEIIYLKGIEKHYNIGQIKVKALQGIYLTISKNEFIVIMGASG
                     SGKTTLMNIIGCLDTPTSGEYYLNNQLVSGLDDDELANIRNKEVGFVFQTFYLLPRYN
                     ALENVMLPLVYAGVPKKERIERAVAMLDLVGLKDRMYHKPNELSGGQRQRVAIARALI
                     NNPSILLADEPTGNLDSYSSYEIMSLFEKLYNDGNTIVLVTHEPDIAAFGHRIVKLRD
                     GKIISDQANLNIANAEYFLKNYQEI"
     gene            32388..32945
                     /locus_tag="PKI65_00130"
     CDS             32388..32945
                     /locus_tag="PKI65_00130"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOB76886.1"
                     /translation="MKNQEKIYTKYGDKGYTQLLSGEKVPKYNLRIEAYGNIDEATAF
                     IGFLYDNIDIVEINDDLLRIISDLFVIEAHLSNKSNSADSLPKFSYTNIQFIEDRIDH
                     YASTLNKLNKFILPIGDFNASFCHIIRTVIRRAERRVCELTDNEVIPNKDLIISYLNR
                     LSDYFFVLSRKILHLNGKEERYWEY"
     gene            33015..34211
                     /locus_tag="PKI65_00135"
     CDS             33015..34211
                     /locus_tag="PKI65_00135"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HOB76887.1"
                     /translation="MINLENIKAFKERIESVGVPILSVYCDTDPSKDENKGMAWMIRI
                     KNALKEYKELEKKLKNRRSFLDEFMLSLQKVHPQTRTLVIFAWVNEKNVMQSETFELQ
                     IDLPAIDLTLGRIDVHYGKPYLLPIYYAFDEYERIGVLHIYGTKWRFYEVFLNEVEEI
                     TDTFATITTDEWKEIQEHLNAIDQTLYGERNVQARIKFKDKFKSKYENWSHKLYNKLA
                     KMIEKSIQTLDINRLILMGTEWQLNFFENYLSHNIKNIIIGHLPNPTNAENPSKKEIL
                     ERVSTVIEKTEYEEEKMLLDLVYQGNGISGLSEVLKALQIGRVQVLLLPWDFNAIAYM
                     CSNGLILATKEEADQYCGDANEIYLRDYIEDLTNEYSTRIEFVKGEIKDKLYTDLGGI
                     AGVTRW"
     gene            34309..35286
                     /locus_tag="PKI65_00140"
     CDS             34309..35286
                     /locus_tag="PKI65_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOB76888.1"
                     /translation="MKTLNIYINYFKKHLNELDLNREPKTLYDPIKYGLGVGGKRFRP
                     ALILLLCDFYGGNLDDAIPAALGIELFHNFTLFHDDIMDKAQLRRGKETVVAKYGLNT
                     ALLAGDTLFAIAYSMVNKTNEKHLNRILEEFTNTATKVCEGQQLDMDYETQINVTKND
                     YEKMIYLKTAVLIASSMKIGAIIGNCPANDENLIYDIGISLGLAFQLQDDYFDCFGNE
                     NTFGKKIGGDIVDAKKTFLYVSACESINNETERKYFYDLYNNKNIDNEEKNKKIITIY
                     KNLNIDKKTIKQIDYHIMEAKDVIHQLSIDNNKKDLISQVIDDISNRNK"
     gene            complement(35314..37332)
                     /locus_tag="PKI65_00145"
     CDS             complement(35314..37332)
                     /locus_tag="PKI65_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB76889.1"
                     /translation="MKQIIFFLFLVIFPHYIFTQVSIPELRCLEVLNNGDVSINWLPS
                     NDVNGDFIAYEIFYSVNINGPYTLISTINNINTTNYLHVSAQANMHKVHYYIKAKYGS
                     PINYTENSDTLSTIYLSLTDPLNGTAILSWNELHQPPLTSFSNYEILREYPSGIWSTI
                     GNTISTNFIDTIYPCSASISYQIKQIDNSGCYSLSNISSGIFHNLMPPKSPFLDSVTV
                     LSNGSAALGWQPSNSADVIAYIIYKYESGFWTPIDTVSGTNYYVYSDSNADESAEQYR
                     VAALDSCGNTSPLSNAIETIFLNGSLDICNNIFYLSWNHPTGVLNIEKYKIFLSKNTQ
                     PFIEISNTIRDSFNLPIDDTVGYWCVFIQSINNLGNTQSSNVLCFNFKKPSLPAYVKF
                     RKADFINNRINISWKCPPHTSTLGFSLYRSTDSLISFKKIATIQKNLTGDYIFVDPLT
                     NYTSYPIFYTLATIDSCGNEVFQSDTVSTICLNGTVNFDITNSLFWQGKASSSVINLQ
                     QILHKKIDNQPYLELNLPFDQRTYLDDLVDLEDIESSGHFSYQIEWIVYLDNPFDDYD
                     TIHSLWLDLFQPPRIYIPTGFTPNSHLDVNKIFKPSGIFLKAKDYSFTIFDRNGRIVF
                     ETNDLDKGWDGNSFDGKPEPEQLYVYLLKITLINGKVFEKRGIVMLYR"
     gene            complement(37457..37774)
                     /gene="trxA"
                     /locus_tag="PKI65_00150"
     CDS             complement(37457..37774)
                     /gene="trxA"
                     /locus_tag="PKI65_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOB76890.1"
                     /translation="MIENLTEINFDKVISEAKVPVIIDFWAEWCGPCKMMGQVLEEID
                     KIYNDKLFIGKVNVDESGSIAMKYGIRNIPTLLFFKNGQLVDKQVGAVTKKMLEDKLK
                     KLL"
     gene            38240..39226
                     /locus_tag="PKI65_00155"
     CDS             38240..39226
                     /locus_tag="PKI65_00155"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB76891.1"
                     /translation="MDNFNLLDYQALPKEQKRRFLDNLYQFLLENNYTAVDYQKLKIQ
                     STAPICPRCESENVIKAGVRDGKQVYKCKDCGRQYRETARTFVYRMRKADKMLDYLKC
                     MLEGKSLRACASEVGISLRTSFNWRHKILAAIKSLQGGISFSGIVEADELLMKYSEKG
                     RKYRSRKEYEKAKKKKHPKVAVLVMTDREGNLLFKHVGRKKVTNSQIREELSHRVTDS
                     NLICVKQKKEFKKAVKGTKSKKVVVNKTQIRRGVYSVQVVDKHVSDFKVWLRRFRGVA
                     TKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSDRWAWDEYWRLVNTKYR"
     gene            39519..40094
                     /locus_tag="PKI65_00160"
     CDS             39519..40094
                     /locus_tag="PKI65_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76892.1"
                     /translation="MKKIIFVCLTLFLFVNIHSQTLDMWEFGSGLSKAFDLGGELGIT
                     FGTNTNIMVSIKGAYHFNNYFSLGLNPFYWYIKSHLYTPAISINMTGIRGYLDISVFR
                     ALYLHCEFEKMFYRAPSLVYPYSEKWFNLDNFLVGAGFRQFISYNTYSYIEVLWNLNE
                     NINSVYYSPVIRAGVSYAFPNKKRYNKSDLE"
     gene            complement(40080..40460)
                     /locus_tag="PKI65_00165"
     CDS             complement(40080..40460)
                     /locus_tag="PKI65_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOB76893.1"
                     /translation="MLFFLSKNLISCAREIFINWYKAKALSEITNRAFYYSNKYGFEF
                     SKIKISNAKTRWGSCSHKGNIYINWTLIMAPEDILDYVIIHELVHLEIPNHSKAFWNR
                     LEIIYPNFIEAKKWLRDNANLLKI"
     gene            complement(40441..40755)
                     /locus_tag="PKI65_00170"
     CDS             complement(40441..40755)
                     /locus_tag="PKI65_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOB76894.1"
                     /translation="MNEIKIDKIIRSKRSTVSIQINEDNKLVVRAPYKLNENEIYKIV
                     QKHKKWIEKKQKEIEERNKKYIPKKFINGEQFLYLGNYYPLIILNEQNQSLIFDNAFF
                     FI"
     gene            complement(40935..42830)
                     /gene="rpsA"
                     /locus_tag="PKI65_00175"
     CDS             complement(40935..42830)
                     /gene="rpsA"
                     /locus_tag="PKI65_00175"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOB76895.1"
                     /translation="MENEKNFQPEMNQNEVDKTEEISNESEALSIDEDVKLIEEASDA
                     VEAKQENTENDDFDWDALLKETREYKEEERKKLEEKYTASMNVLQDKEVIEGTVIGFG
                     EREVFVNINYKSDGVIPLSEVRYNPDLKIGDKIEVYVESQDDGTGQLILSHKKARILS
                     AWNKINELYDKNEIVQGYIKSRTKGGLIVDLFGIDCFLPGSQIDIKPIKDYDAFVGTT
                     MDFKIVKINQEFKNVVVSHKAIIEEEIEAQKSEIISKLEKGQVLEGEVKNITSYGVFI
                     DLGGIDGLIHITDLSWGRINHPEEIVQLEQKLNVVILDFDEHKKRIALGLKQLTPHPW
                     DLLDPNLKAGDKVSGKIVAIADYGAFIEIIPGVEGLIHVSEMSWSQHLRSAQDFLKTG
                     DTVEAIILSIDREERKMSLGIKQLLPDPWVNIKEKYPVGSKHKAKVRNFTTFGVFVEL
                     EEGVDGLIHISDLSWTKKIKHPSEFCKIGDELEVVVLEIDTDNRRLSLGHKQIEENPW
                     EVYETVFTPDSIHQGKIIESRKDKGAIIELPYGMEAYAPASQLIKEDKTKAKSGETLD
                     FMIVEFNKDTKKIIVSHTRTWETPKPSKSKKTKTAQSDSSTHTTLADLGIFDDIKEQL
                     NNNEDKK"
     gene            43026..43439
                     /gene="mce"
                     /locus_tag="PKI65_00180"
     CDS             43026..43439
                     /gene="mce"
                     /locus_tag="PKI65_00180"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOB76896.1"
                     /translation="MNLTHIEHIGIAVKNLQESIKKFENMLGIKCYAIEEVKDQKVKT
                     AFFQIGQNKIELLEGTEPDSTISKFIEKRGEGVHHVALAVNNIEGALQELKEKNIQLI
                     DEKPRLGAEGLHIAFIHPKSAGGVLIELCENKNDK"
     gene            43449..45008
                     /locus_tag="PKI65_00185"
     CDS             43449..45008
                     /locus_tag="PKI65_00185"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOB76897.1"
                     /translation="MSIEKKIEELLEKRKQALLGGGQKRIEAQHSKGKLTARERINIL
                     LDEGSFEEFDMFVTHRCTDFGLEKELYYGDGVVTGYGTIDGRLVYVFSQDFTVFGGSL
                     SESYAQKICKIMDLAMENGAPVIGINDSGGARIQEGVRSLAGYADIFERNILASGVIP
                     QISAVFGPCAGGAVYSPALTDFIVMSKKNSFMFVTGPKVVKTVTGEIVTDEELGGPDV
                     HANKSGVAHFVSETEEEGLLLIRKLFSYLPSNNLEDPPFVDTGDPIDRLDDYLNYIIP
                     ENPNKPYDIKDIITRIVDNGEFLEVHAQYAQNIVVGFARLGGMSVGIVANQPNYLAGV
                     LDINASRKGARFVRFCDAFNIPIITLVDVPGFMPGTTQEYGGIIVHGAKLLFAYGEAT
                     VPKITVTLRKSYGGAHDVMGCKQLRADINYAWPTAEIAVMGASAAIEILERKNILAIQ
                     DEEERKKYIEEKINEYTEKFCSPYEAARYGYIDDVIEPRNTRFRIIRALYSLSTKKQN
                     LPPKKHSNCPL"
     gene            45020..45952
                     /locus_tag="PKI65_00190"
     CDS             45020..45952
                     /locus_tag="PKI65_00190"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOB76898.1"
                     /translation="MKRVVLFFMLLLLAMTNFSQNISDIRINEISPNYDGDLTNPLGN
                     KSPWIELYNTSLVSLNIGTMYLTDDRENLKKYQLPKSDVFNISPNGFLVLYLDTTEHC
                     HPLVAPLSTKTGYLALVLSNGRTIIDSLVFDPSAIEHETFLKRKIDGTGEWTLTKIST
                     PLLSNDIKVYETPDEIVKKLDPYGIGISIICFSIIIIILIILYLAFYYIMQIKKPIAK
                     IKKIEIKKEGETVKTEIIQAEEEISSEVVAAIGTALHLYLLSIHDYESTVITIKKIQK
                     PYSPWSSKLYNLTPTPQRIHLPKKFIYEKNRSNN"
     gene            45927..46355
                     /locus_tag="PKI65_00195"
     CDS             45927..46355
                     /locus_tag="PKI65_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOB76899.1"
                     /translation="MKKIDLTINGNKYSVEIGNVQDNRVEVNVNGTIYEVELSKEIEI
                     PKTPTLVSTVVNKSVKETIPEPVEVMATKQIRSPLPGTVLNILVREGDTVQRGQNLLI
                     IEAMKMENEIKADSAGIVKKIHVHKGDTISEGQILVEIGG"
     gene            46359..47513
                     /locus_tag="PKI65_00200"
     CDS             46359..47513
                     /locus_tag="PKI65_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOB76900.1"
                     /translation="MDFLGFVTQNLEKFLNYTAFANMSWGNVIMILVGLFFIALAITK
                     QYEPYLLIPIGFGIILGNIPSVPGLQIGIYEEGSVLNYLYFGVIKGIYPPLIFLGIGA
                     MTDFSALLSNPKLFIIGAAAQLGIYGALILALLFGYTPTEAGAIGIIGGADGPTAIFL
                     SSRLAPHLIGAIAISAYSYMALVPVLQPPIMRAMTTKKERTIRMKPPRAISKKEKVLF
                     PILGILLTTLIVPSGLPLLGMLFFGNLLRESGVTRRLAETASGPMLDIVTILIGLTVG
                     ASTQASTFLTVKSLGIFALGALSFMIATFGGVLGVKIMNLFIKKPENKINPLIGNAGV
                     SAVPDAARVSEMIGLEYDPSNHLLMHAMGPNVAGVIGSAVAAGLLLSFLA"
     gene            47601..48086
                     /gene="purE"
                     /locus_tag="PKI65_00205"
     CDS             47601..48086
                     /gene="purE"
                     /locus_tag="PKI65_00205"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOB76901.1"
                     /translation="MISIIIGSISDFDIIKKAIDLLEDFNVEYDVQILSAHRTPDETA
                     KYVKEATEKGTKVFIAAAGLSAHLPGVVAANTIVPVIGIPINTSLQGIDSLLSIVQMP
                     SGIAVATVGINAAQNAALLAIEILAINDVKLKQKLIDYKNKMKEKIAKDQKELEKKLS
                     K"
     gene            48093..48944
                     /locus_tag="PKI65_00210"
     CDS             48093..48944
                     /locus_tag="PKI65_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76902.1"
                     /translation="MRKILLFSLVLILSINTLSGQFSYESASTLGYGNSASAVPGFWS
                     GLMNPAGLGWLNNHQLGINYYNKYLLKELGYRDIAYAHVFNGDAGVISAHISQFGASS
                     LLYNTFSLGYSRVFADRVSAGLTIKYFLLTSNQPEYSGIGTIGFDAGLQVKIIDNLYF
                     GTMIANLNHSKLGTYEYDRLPIVGSLGFMYQIAKQSSLMLDILKESNNNPSLRFGTNF
                     SFNDRLYVRGGFQTTPARVSFGIGYRIQNFIVDISSSWHQTLGFSPSISMSYAFKKEH
                     QKPNIIY"
     gene            48947..51040
                     /locus_tag="PKI65_00215"
     CDS             48947..51040
                     /locus_tag="PKI65_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76903.1"
                     /translation="MKAKIYIFFVFICFFSYLSGQVEIDEPEELDIIQQNIEYIAEES
                     GVEEADFSELTEKLEYYLKHPINLNKTTVDELRDLGLLTDIQIINFFDHIQRNGKLHS
                     LYELQSIEGWDLATINKILPFVKISDVGVAHKYNFSEVMKEGNSQLFIRYMQTLEKQK
                     GYKAIDDSTLAANPNSRYLGSPYKLYTRYQFKYYNLISFGFTAEKDPGEEFFKGTQKQ
                     GFDFYSAHFFIRNVGPFKAIALGDYNLQLGQGLTMWTGMSFGKTADAVFTKRNATGIK
                     QYTSADENRFLRGVALNAKISKRLEILGFVSYKKIDASVEGDTMSYEDYYISSIVETG
                     LHNTVSSYNKKDAIKQFIGGGNIQYTSLNKRLRIGATAFTTILDKPLGSRDELYRMYE
                     FSGTTLNVASIDYSYIWKNFNIFGEEAYSSNGGIATINGIMAVTDKNLSFSLLHRYFD
                     PKYFVFYASPLQEGSKASNEQGLYFGLNANLTHKLNLTAYFDLFSFPWLRYRVNAPSE
                     GMEALVQLNYKFDKKNSIYFRYKHETKYLNKDDLYVKKLIETKKDNYRLNGVFQLSPS
                     FRYKFRVDYVNYRKGDDYNHDGFAVSQTLSYKKGRFPLSFHLTFIYFDTYSYDERIYG
                     YENDVLYAYSFPSYYYKGARYVINIKYTINKYLDFWAYLARTTYFDRNVIGSGLNEIQ
                     GNHKTDLHLQLRVKF"
     gene            51272..52282
                     /locus_tag="PKI65_00220"
     CDS             51272..52282
                     /locus_tag="PKI65_00220"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB76904.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEIVTSNRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNITYLNLN
                     IY"
     gene            52661..53965
                     /locus_tag="PKI65_00225"
     CDS             52661..53965
                     /locus_tag="PKI65_00225"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOB76905.1"
                     /translation="MLSSEERKEIKNLIQSEITPAIGCTEPIAVALCVAKAKEIIGSI
                     PEKIDVLLSKNIIKNAMGVGIPGTGMVGLPIAIALGALIGKSENKLQLLKDVQPRDVE
                     KGKKYISENRINIYLKENCPNNLYIETHCYVDSEKSVVIIEGAHDNFTYIEKNNKILL
                     NQSYESNDKNDNVVQLTFNKIYEYAIETPIEELEFILEAADMNKLASIQSFIGNYGHQ
                     ISKILTSEKAKSILGDNILNRIIAATAGACDLRMDGAMVPVMSTAGSGNQGIAATIPV
                     LIFAEETNCDKKKLIRALILSNLTVIYIKQKIGKLSPLCGCVVASTGSSCAITYLMNG
                     NKLQISHAAKNMIASITGLICDGAKPSCSLKIATSIYTALLSSLMAIDNKVVLSNEGI
                     IDDDIDKTINNLAAIGNIGMKNTDNIILDIMTKKISKSPMKY"
     gene            54011..55051
                     /gene="ribD"
                     /locus_tag="PKI65_00230"
     CDS             54011..55051
                     /gene="ribD"
                     /locus_tag="PKI65_00230"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOB76906.1"
                     /translation="MCENDLIYMRRCLNLAKEGLGYVRSNPLVGCVIVKDNHIIGEGF
                     HHAFGKPHAEIEAINNVKDKSQIKNSTIYINLEPCSHYGKTPPCSQSIIDVQPLRLVI
                     ADIDPNPLVNNKGKEILEKAGIKVDVGLCKNEHRHLNRRFYTFYQKNRPYIILKWAQT
                     LDGFIAQKNQQCIKWISNDASLQLVHKWRTEEMAILVGAETIRCDNPQLTSRHWYGHN
                     PIRLVVTNSSNLPINSNIFNDQAETTIFYKNLIKQYPSNIDLVKIENDFLGSLFAYCL
                     EKNIISILVEGGASTLDNFIKRNLWDEARVIIGSNIFGNGKKAPIIDEYKSFDIFDLE
                     DNKIIHYYNDTY"
     gene            55246..55680
                     /gene="dut"
                     /locus_tag="PKI65_00235"
     CDS             55246..55680
                     /gene="dut"
                     /locus_tag="PKI65_00235"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOB76907.1"
                     /translation="MIQINVKNISHHPLPDYATLFSSGMDIYAYIDNEFVMKPMERAL
                     ISTGLYIELPPGYEAQIRPRSGLAIKNGITVLNTPGTIDADYRGEVKVILINLSDQNF
                     TIHDGDRIAQMVIQKIERALWREVDEISDSERGGGGFGHTGK"
     gene            55704..56711
                     /locus_tag="PKI65_00240"
     CDS             55704..56711
                     /locus_tag="PKI65_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOB76908.1"
                     /translation="MKIIIPMAGIGKRMRPHTLTIPKPLIPLAGKPIVERIINILIES
                     LDREIDEIAFVIGDFSEEIQQRVADLGNKFNIKTSIYRQSSPLGTAHAVLAAEPSLKG
                     EVIVAFADTLFFSQFNLKNMDADGIIWTCEVPNPKDYGVVKLDDDGCVTEFFEKPTNF
                     ISNKAIIGLYYFRNAEILKKSLFKLIENDQKVNGEYQLTDALQYMIENGYKIKAQNVD
                     EWLDCGNKNATLIAHKRILERYPDESKVNESVKCKNAIIVPPCYIEEDVILENSIVGP
                     FVSIGKDCIISHSIITNSIIQNNVKLSNVNIDNSMIGKYVDYNDSYDELNIGDYSEMN
                     K"
     gene            complement(56780..57319)
                     /locus_tag="PKI65_00245"
     CDS             complement(56780..57319)
                     /locus_tag="PKI65_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76909.1"
                     /translation="MSTFKKTLFAFLIFFIQILILDHLPLSWGIQINLFIYIFLCLIP
                     FNYNKTLLLLIGFAIGMLRDLFTFTYGLYAFASTLLMFLQYYYFRLPQFNKYRKADKL
                     LYANSDLLGWPGFLLYITIFTIIFSFTTAIIEYFTTWMYLRLLFIIKEIFFSILFICI
                     AELIRSLLFHKEAIGRASE"
     gene            complement(57324..57971)
                     /gene="mreC"
                     /locus_tag="PKI65_00250"
     CDS             complement(57324..57971)
                     /gene="mreC"
                     /locus_tag="PKI65_00250"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOB76910.1"
                     /translation="MHLKKENEALFYENINLYNKNNQYVNIRCISDNQVDYLISDTSK
                     QLYQFLPARLISMTLHESKNYAVIDKGKRDGIKENSGVFTPKGILGVITWVSERYSLV
                     KTYMHPDVKISAAIKNNYVGIAQWDMHTPNNGEIINVPFTVKININDTIYTSHYSTLF
                     PPGIPIGIITAKTSDNIYQTKRYKFRYLEDLISVYYTYTVVCLDSDEINILYQNK"
     gene            complement(58155..59174)
                     /locus_tag="PKI65_00255"
     CDS             complement(58155..59174)
                     /locus_tag="PKI65_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOB76911.1"
                     /translation="MALFNFLTKEIAVDLGTANTIILYNDKVVVNEPSIVAIDNITNT
                     VIAVGKKAMMMHGKTHEHIKTIIPLRDGVIADFYAAEIMIREFIKMIPWKNTIINYSY
                     RMVIGIPSGITEVEMRAVHDSAEQAGAKEVKLIHEPMAAALGIGIDVLDNYGNMIVDI
                     GGGTTEVAVIALGGIVSNKSIRIGGNELNEDIQDYMRRAHNLSIGERTAERIKIEVGA
                     AIPNIENPPEPMEVNGRDIITGIPRSVHVNHEEIAHAIDKTISKIEDTVINALEYTPP
                     ELSADILQNGIYLTGGGALLRGLDKRLSTRIKLKVKVGDDPLLAVARGASVALKNFNK
                     FPFLI"
     gene            complement(59191..60714)
                     /gene="purH"
                     /locus_tag="PKI65_00260"
     CDS             complement(59191..60714)
                     /gene="purH"
                     /locus_tag="PKI65_00260"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOB76912.1"
                     /translation="MSIKTINSVLISVYNKDVLLPYLNILKNYKGKIISTGGTSKFLN
                     ENGINVIEIDNLTNYPALLDGRVKTLHPVIIGGILLRRDNENDKKEAKKYNLEEIDAV
                     LVDLYPFAETIKTSIDESEIIEKIDIGGITLIRAAAKNFKDVLVAADSRDYPHFFECI
                     KNGYSEEHDRRHLAKNSFAIVTMYDATIFQWFSQPYLAQESKIISKNLVTPLRYGENP
                     HQQAFFLHDESMRYEKHHGKELSYNNFLDIDSAWLLVNELNQPSCVVVKHTNPCGVAI
                     GKSPIDAFDRALAGDPVSAYGGIFAFNTPIDDATIEKLNSLFFEVLIVPHFTKGQIEK
                     LSIKKNRILLSISETKPNNWQWRSVLGGSLVQQRDDYIPDSSKWEWIVKNEKLNNKLI
                     DDMKLGVSIVKYLKSNAITIVKDNQLLGMGCGQPSRVDSTRIAIEKAKTFGHDLTGAI
                     LASDGFFPFTDSIDLAYQNGIVAVVEPGGSIHDKNIISFCQENDIILSFTKIRNFKH"
     gene            complement(60855..61427)
                     /gene="rsxA"
                     /locus_tag="PKI65_00265"
     CDS             complement(60855..61427)
                     /gene="rsxA"
                     /locus_tag="PKI65_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOB76913.1"
                     /translation="MEYLIIIISAIFVSNVVLTQFLGICPFLGVSQKMSTAVGMGAAV
                     VFVLTLATLVTYLVYYGILAPLHLEYLQTIAFILVIASLVQMVEIIIKKISQPLYQAL
                     GIYLPLITTNCAVLGVALLVITKNYNLGESIVYAISIGIGFGLALIIMAGLRERLEFS
                     NIPKAFQGVPIALILAGILSLAFMGFTGII"
     gene            complement(61430..62041)
                     /locus_tag="PKI65_00270"
     CDS             complement(61430..62041)
                     /locus_tag="PKI65_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOB76914.1"
                     /translation="MNQWQNFSKGILKENPVLVLVLGMCPTLAVTSSAINGLAMGLAF
                     TFVLTLSNVIISSIRKIVPKIIRIPVFIVIIATFVTIIDMVMHAFVPDLYKALGIFIP
                     LIVVNCIVLGRAEAFASKNNVLSSLIDGLGMGVGFTLILTVLGMIREILGAGSIFGYK
                     FIAPTSDGILVFILAPGAFLTLGILMALNNLYKNKMSNKPKNN"
     gene            complement(62046..62618)
                     /locus_tag="PKI65_00275"
     CDS             complement(62046..62618)
                     /locus_tag="PKI65_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOB76915.1"
                     /translation="MSKRESSLSNLALTLFIIAAVAAIMLALINLITTGPIQKAQQQK
                     EKDALREVLPSFSDLKTFYIPNIDGEDSLKVFEASYNGQIVGYAVSTYTMQGFNGKME
                     LMVGFDTTLTIINTQVLSHAETPGLGEKITQSDFKDQFRGKNPEKFKLSVKKDNGDVD
                     AITASTISSRAFCDAVDRAYRSLKAWKGGE"
     gene            complement(62615..63601)
                     /locus_tag="PKI65_00280"
     CDS             complement(62615..63601)
                     /locus_tag="PKI65_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOB76916.1"
                     /translation="MDKKFIISGSPHIYAKDNVQRIMLDVVIALIPATLGAFYFFGLG
                     AVRVFFVSVVTCLIFEYLIQKFLLKQKPTINDLSAIVTGMLLAFNVPSNLPTWVVIIG
                     SLIAIGIGKMVFGGLGQNLFNPALVARVFLLISFPVYMTSWPKPIASAGQLSDVVTGP
                     TPLGILKESGSISAASQTGELPSYVDMMLGNMGGSMGEVSAILLLIGAIYLLYRKVIT
                     LHIPLSYILTVIIFTGIFWLIDPTKYADPLFHLLTGGLILGAFFMATDMVTSPTSYRG
                     QIIFGIGCGVLTALIRLFGAYPEGVSFSILIMNAFVPLIDRISYKHFGEVKA"
     gene            complement(63609..64943)
                     /gene="rsxC"
                     /locus_tag="PKI65_00285"
     CDS             complement(63609..64943)
                     /gene="rsxC"
                     /locus_tag="PKI65_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOB76917.1"
                     /translation="MLKTFPKGGVHPDDCKLTAKSPIFVATLPDKVYIMLNQSAGAPA
                     VPIVKKGDKVKTGQLIAKGEAFISSNVHSPVTGTIDKIDPILDATGYKHQAIIINVEG
                     EDTWEEGIDITNNLIKEIKATPQEIIEKIKNAGIVGLGGAAFPTHVKLTIPQGKVAEY
                     LLINGAECEPYLTCDHRLMLEKPEEILVGTSIIMKALGVHRAFIGIENNKLDAIELLT
                     KLAKDYNGIEVVTLKKKYPQGAEKQLIKAITGREVPSGKLPIEVGCVVQNVGTAKAIY
                     DAVQKNMPLIERVVTVTGPNVSKPSNFLSRIGIPLKHLIELAGGLPEDTAKLISGGPM
                     MGKTLSNTDIPLTKAMGGILLFPYNLSKRLDPYPCIRCGRCVHACPMGLEPFYLEKLV
                     IKERWEDAEKHWIIDCMECGSCQYICPAKRPLLDYIRLGKNKVSAIIRSRKN"
     gene            complement(64947..65822)
                     /locus_tag="PKI65_00290"
     CDS             complement(64947..65822)
                     /locus_tag="PKI65_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOB76918.1"
                     /translation="MEPVIIAFVVLSLMAAIFAVIIYFVDKQFHVEEDPRIDLIAEKL
                     PGANCGGCGYAGCRNFAEAIVKNNSLDGLFCAPGGNKVMSEIAELMNLTAEEKKPQVA
                     VIRCQGSFENCPPKHDYNGIKNCGFANFLFAGKGGCAYGCLGLGDCVEACKFDALYMD
                     PITGLPVVIEDNCTACGNCVKACPRNIIELRNKGPKNRRIYVACMNKEKGIYAKANCL
                     VACIGCNACAKECKFDAIKIENFLAYIDYKKCTLCRKCVAVCPTNCILEVNFLPKKQE
                     NANIANDSSKNIVNN"
     gene            66069..66707
                     /locus_tag="PKI65_00295"
     CDS             66069..66707
                     /locus_tag="PKI65_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOB76919.1"
                     /translation="MIVNAFLKGIPVGLILAFSFGPAFFLVIQTALEKGFKKAIVTSI
                     GVSSSDAVFICLSLLGITAIEGLLKDVFGVIASIVLIIYGVVMIFSKPEILKKRNPKV
                     KDIRSKNEYIKLYFKGFLLNIANPFVLIFWVSIAGIVSQTAPKDQFLLYNIFFFLGIL
                     FTVFSMDVIKSLTAHKLKNYLRPRYIIYANRIIGCIIVILGVLLIFKTFMIN"
     gene            complement(66779..67438)
                     /locus_tag="PKI65_00300"
     CDS             complement(66779..67438)
                     /locus_tag="PKI65_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOB76920.1"
                     /translation="MKKFLIILLLLISNLSFAQRNVYSIKIALLKYSGGGDWYANPTS
                     LPNLAVFCNQNINTNIDPNIPEIEPGSQEIFNYPFIHMTGHGNVIFSDNEAANIRKYL
                     EAGGFLHIDDNYGMDPFIRPQIQKIFPDLPLQELPPSHPIFSQVYNFSNGLPKIHEHD
                     SKPPQAFGIILNGRLVLLYTYECDLGDGWESEEVHNDPPEIRLKALQMGANIIHYAFN
                     Q"
     gene            complement(67435..68133)
                     /locus_tag="PKI65_00305"
     CDS             complement(67435..68133)
                     /locus_tag="PKI65_00305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOB76921.1"
                     /translation="MDLFYLENIRDRVVIEGETYKHLRAHHIKTRDKIFFTNGKGLLA
                     TGKIVECMKQKIIVDVLEVKTMPPRDNKAILYTPILKQTERLDWLVEKTVELGVSELI
                     PIITERTIKTTIKLTRLQNIIKYSSLQSLKFYFPHICEPIFFEEAIKNNIKMKRTICY
                     CGTELEKTQFFDFLQPKEDIGIFIGPEGDFTIDEVNKAIYNGIIPVSLGKQRLRSETA
                     AIVSLTIFAIKNNL"
     gene            complement(68330..69283)
                     /locus_tag="PKI65_00310"
     CDS             complement(68330..69283)
                     /locus_tag="PKI65_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76922.1"
                     /translation="MIKRKGKHSNICLKVSDKLILFLSLSTYIIVSYILMNTSAIIYK
                     FRFINSIAISAFLLFVVITIFSFILIYDKKYKYSFSYIIAMLPSFAFFLIFINFDTFF
                     ESTIISYIWFTFQLYLLKKNNFSLKELYFYGLIIFLLIFINPVFIFLLLSVYITISFL
                     DILDFKKIITPFISALTLAIIILTIDYAFLDFQIYHYFLSSKPQLSSASLSLPLLAFT
                     IFSLVTCIMIYPWMQRQLVYKRRQIWVSIVQILICIIIAFTLPLDNFKIVLLIYVWLL
                     TLNIQKILIAEDLTWRKIIYQLFVILISEFWIFSCDFLNLF"
     gene            69786..71153
                     /locus_tag="PKI65_00315"
     CDS             69786..71153
                     /locus_tag="PKI65_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOB76923.1"
                     /translation="MCILILLFLPTNKKPYYQHKYQKLDFPDIIERGYINVIVEHNSM
                     NFFVHHGRVMGLQYELLDHIEKRWNININQIVINDLGEALALVAFDSIDILTSDITIV
                     PGRYPCIDFSIPLYKTKMVLVLYDSIKPSTEINDFSKLDIKKLENKIIAVPTKSIFIN
                     FLNKLKKSLNLNFQIKELIEEPESILDSVMAKKYDATIMDEKILIVNTPLLKNTSSYY
                     LPFPSLDVGWAFSCHQPILRDSVNAALIDLKNKGILASLLNKYQRPIYIQTTYKQIAH
                     SKRTKKLTKYDNILQKYAKIYDIDWRLVAALIYHESNFNIDAVSYKGAFGIIQIMPQT
                     AEEYGMSPTSSIDQQIKVAYRILNDYKKQLSIYFDNDKDLYRAMIASYNLGLGHILDA
                     IALALKYDGQINSWEQVAQWLLNKSQKIYYSDPVVKNGYCKGRLTVYFVNLVYETYEN
                     YKQLI"
     gene            71176..71796
                     /locus_tag="PKI65_00320"
     CDS             71176..71796
                     /locus_tag="PKI65_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOB76924.1"
                     /translation="MNLEFYIPEIIQKEAEAIKNIPIDNKYLDTVDLIYDRVHLKKGK
                     LVTSGMGKAGQIALNIATTFSSTGTPSIFLHPADAQHGDLGVIQPNDVLLLISNSGKT
                     TEILQLVDLARNLYNDIPLIVITGNKDGILAHKADLTLYTGNPPEVCPLGLTPTTSTT
                     VMTVIGDILVTLMMRKIDFKVEEYAKRHHSGYLGEKSKMFVNQNKK"
     gene            71804..72577
                     /gene="kdsA"
                     /locus_tag="PKI65_00325"
     CDS             71804..72577
                     /gene="kdsA"
                     /locus_tag="PKI65_00325"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:TIGR01362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOB76925.1"
                     /translation="MWLFAGPCVIENDKIPFVVAEKIKNIADELGYHFIFKSSYLKAN
                     RSKLDSFRGIPRLEALKILNEIRKNYKIPTITDVHESWECEEVAEYVDYLQIPAFLCR
                     QTELLISAGKTGKPINIKKGQFMSPESMKYAVDKVKSTGNENIFLTERGVMFGYYDLI
                     VDMTSIPRMKSIGCPVLIDATHGIQMPNRSDGVTHGHREFVETIALAGIAAGADGIFL
                     ETHPNPEQALSDADTQWPLDDVYDLLKKAKEIYSIINNV"
     gene            72594..74447
                     /locus_tag="PKI65_00330"
     CDS             72594..74447
                     /locus_tag="PKI65_00330"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOB76926.1"
                     /translation="MSLLILIYPFFVYNQIFITPEPQSMTKRIDIFVGQGYDIKYHKL
                     YISVTPGSDHISGGVFTKFIPLDDTDTMDFDANQCLVIDSVIYHGNAMTFIHQNNRLI
                     IKNLTLVSQSLDSIIIYYHGTPAASGFGSFYSDSQWLWTLSEPFGASDWWPCKNDLSD
                     KIDSIDIFIEHPSNYIAVANGLEQSKVDLVNSRTLTHWKHKYPVATYLIAIAVAPYHY
                     FNVHIPLTEGDLQIDNYVLSSAYNNAQNAMPNFSKVMKFYDSLIAPYPFMNERYGHAH
                     FGWGGGMEHQTISFVGSFDYELLAHELLHQWYGDAVTCGSWHDVWLNEGFATYFTALS
                     QEKFFPQDFIYWKNQSIDFITYLPCGAVYVYDTTSVDRIFNSRLSYRKGAYILHMLRW
                     ILGDDVFFNGVRQYYQTYKYNSAKTEDFKKIMEQVSDTTLTEFFNDWIYDEGYPMYNF
                     TVTNIDDDNFKIQVHQSSSCHYNTFFEMPLPFLLRYDIFDTIIILNNLVQNQDFFINL
                     PMPNEIIFDPNKWILTKNATIIIDVETYLNKQLSIYPNPASNKVAINNHSNNKFTLVI
                     TDLLSKKLIVDDCILPNSTYTVDISSLITGTYLMTFKSEKNEIITKKLVKQ"
BASE COUNT        26074 a        10591 c        11123 g        26808 t
ORIGIN      
        1 aaaattaaca attaatttta tattaatcca atattaaatt aaacaattga aattttccct
       61 atgaaaatcc taagctactt aatagtaatc taaagtattg cgtattggta aaaaaaacat
      121 attatttact tgcatcatta tgttaaaaat cgaattacaa attataattc aaaaaaagta
      181 aaatcttata ttattaagtt agagatttat tcaaatctat atttattttt ccaaagtttt
      241 tttaagtaat tttttagttc attttcttta gaattatcag caggtatata aaaagaagtc
      301 ccagatattt catctggcaa acactcttgc tcgatgaaat ggttaggatg atcatggggg
      361 tataaataac ctttgtgata attaagcgat cgcataaatt gtgtaggtgc atttctaagg
      421 tgaagcggca ctggcaaatc gccagtttta tgaacaaatt ctatagcttt ctctatggat
      481 aagtagctag cattactttt aggtgatgaa gcgagatata tagcgcattc agctaaaact
      541 atccttgctt ctggcatacc aattttatga actgcatcga agcaagcatt agctaatagt
      601 aatgcattag gattagcgag tcctatatct tcagaagcta atattatcat tctacgtgct
      661 ataaatagtg gatcttcgcc tccttctagc attcttgcca tccaataaat agcagcatta
      721 ggatcactat ttcttataga ttttataaaa gcagagataa gatcatagtg atattcgcct
      781 tgtttgtcgt acaataaaaa tttttgtttt actatagctt ctacattttc attgctaatc
      841 actagatttg tactattgct actatctact aataattcta ttacattata taattttcta
      901 gcatctccac cactataagt aaataaagct tctttttcaa ttacttcaat aattatattt
      961 ttcgatgagt aaaaatcaat agccctttgt aacaatttat ctaaatcaga aatttctaat
     1021 ggatttagta cataaacttg gcagcgagat aaaagaggtg gtatgatctc aaatgaagga
     1081 ttttcagtag tagcacctat cagagttata attccttttt ctactgcatg taataagcta
     1141 tcttgttggg atttagaaaa tctatgtatt tcatctaaaa ataaaataac tgttttatat
     1201 ggttgagctt tttgtattat atttcttata tctttcacgc ccgcgtctat ggcatttaat
     1261 ttgaaaaaag gaacttgtaa atcttttgct attaatcctg caatagttgt ttttcctgtg
     1321 ccgggcgacc cccacaagat taatgaaggt aatgatttag aacttaaaag ttttctaatg
     1381 atgccatctg cccctaccaa atgctcttgt cctacaaact catcccaatt ttttggtctt
     1441 aattgctctg ctaatggaat agacatatta caatattatg caaatataat aaatttaatt
     1501 taatttttta taaaatttat tttgaaaaat tcaatatgtt ggattagtaa aagtaaagct
     1561 gtattagctg cagattctat atttatttcc ctattaccat gaaatacaaa tttgtaacaa
     1621 taaatatttg atggcgtaga aatagcaaaa tgaaccaagc ctacaggttt gtttggtgta
     1681 gctcctgtgg gaccggcgat gccactaata gcgatagaat aatctgtgtt gaataaattt
     1741 ttaccatttt ctgccatata ttttacagtt tcttcgctta cagccccctt agtagttaaa
     1801 atatctttgg gtacttttaa tattttttct tttatctcat tcgaatagca tattaatgaa
     1861 cctctgaaat attcggaact gccagcattc gatactatct tactagctac tattcctcct
     1921 gtgcaactct caatagtaca caaagttgta cctgtattta acattaattt gtgtaaatat
     1981 tgttctggtt tttcaatact tttatataca aaaaactctt gatatttaaa tatcaaatca
     2041 tttaaaaatt gattaaaact attatcatta gtataatctt tattagcaga aaataatata
     2101 cgtacatatc caaaacttgg taaatatgct actgtcaata aatcaaaatc attaatttta
     2161 gattcaatat atgtggctaa ttcactttct cgaatatttc taaaggttaa tgctgttgtc
     2221 catataactg gtgaataatt ttgaataatc caaggcaaaa ctgcttcatc taagacagct
     2281 tgtgcttctt gtggtacacc tggcaaagca aataatgtag tattgccttt agttattttt
     2341 atgccaggag ctgcacctac agaattgggc aataattcag aggtttcagg tattagaaac
     2401 attttttttc tcatctgtgt gatttgcaca tttcgttttt tataatgatt tttaattaat
     2461 tgcttggctt tattattctc tactaaaggt acttctgacc attttgctat tgcttctatt
     2521 gttttgtcat cgggcgtagg acctaagcca ccagtagaaa ttattatttc atgagatttt
     2581 gataattgat taagggtttc tactatttca tctaaattat cagaaataga tattattgtt
     2641 tttgtattaa atccattcat aaataattta ttagctatat acgaagcatt cttattatct
     2701 attatacctc ttaatatctc atctcctata ctaagtattg ctatattcat aattattaca
     2761 ttaaaaggat gtaaacaaaa atactaaaaa atatttttcc gaacaatcat atattatgta
     2821 ttttgtagca tgcaatatga aagtaataga ttaaaattac gaaaaaaact taaataaatt
     2881 acaattaatc tctttatata aaatcgaaat aaactcaaat accttaaatt ctgaattatg
     2941 taaattgttg ttttttcaat tgtttatgat tagcatttat ttaacagatt aaaaaatttt
     3001 agattagaag ctaaattatt tatttggtta atattaatta gattaagcta aatatttatt
     3061 aattttgcta acttttaata taatttttta ttaataatta taatgaattt tgaaattata
     3121 tcacaatgtg gtaatgcgag agctggtatt ttgagattac cacatggcaa tgtgcagaca
     3181 ccagtattta tgccgatagg cacagtcggg tcagtgaaag gcattatgtg ggatgatcta
     3241 aaaaacattt taaaagttca aataattttg gcaaatacct atcatctcta cctaagacct
     3301 ggtatagata tactgtatga agcaggcggt ttgcacaaat ttattaactg gcaacgtccc
     3361 attctaagtg atagtggtgg atatcaagtg tattcattag caaatattag aaaaatcaat
     3421 gatgatggag tagagtttcg atctcatata gatggcagta agcacatatt tacaccagaa
     3481 aaagtaatga aaatacagca atatattggt tcagatatta taatggcttt cgatgaatgt
     3541 actccatatc ctgtagatta tgaatatgcc aaaaattcat taaatttaac acataagtgg
     3601 ttagaacgtt gcattgatta tctggagcat aatccgccat tgtatggtta tatgcaattt
     3661 ttattcccaa ttgttcaagg cagtatttat tctgatttac gagaagcttc agccttattt
     3721 gtaagtaaat ttgatacgtt aggctatgct ataggaggtt taagcgtagg cgaaccagct
     3781 gatattatgt atgagatgat caaaatagta aataatattt taccacataa taagcctaga
     3841 tatttgatgg gcgttgggac tccagccaat atattgcaag cagtagcttt aggtgtagac
     3901 atgtttgatt gtgtaatacc tactcgaaat ggtcgtaatg gtcaattatt tacatctcaa
     3961 ggtattatta atataaaaaa taaaaaatgg agtagggatt tttctcctat agatcttaat
     4021 agcccattag aattagatca aacaactaca aaagcatatc taaggcattt attccttgct
     4081 ggagaaatgc taggtcctat gatagcttct tatcataatt tagcatttta cataagatta
     4141 atgaacgaaa tacgttatca tattttagaa ggtagctacg atcaatatta tattaaagca
     4201 atggatactt atatgaaaag attatgaaaa tcaaaaaatt agatttatat attgctaaaa
     4261 aatttattag ttcttttata ttaattatta ctttattaat ttttatcatt attgcttttg
     4321 atatttctga aaaaatagat aaatttattg caaagaatgc tccagtttca cttattatta
     4381 cagaatatta ttttaatttc ataccatatt tcataaatct tttttcatca ctatttattt
     4441 ttatatcagt aatatatttt acatctaaat tagctagtcg ttcagagata atagctatgt
     4501 atagcgtcgg tattgatttt aaaagaattc tcagaccata tctagtagtt agtttttgtt
     4561 tagctatttt ttcatttgta ttgcaaaatt ttattttgcc acctgctaat caacgtagaa
     4621 taaacttcga aaatgtatat ataaaaagaa atcctcagac caaagaaagt tatattcata
     4681 tgcaattaga taaaaacaat tttgtatata tgcatacatg ggatgtaaaa gattatgagg
     4741 gtagattttt atcaatggat aaaatcgact atcaaaaagg tgtaactaat aaaatgatag
     4801 cttcttttag tcattatgat acaatttcta aaaaatggca attgacagat gtaatcatta
     4861 gaaatataaa agatagtata gagcaattaa ccagatacaa aaacttagat acgatattac
     4921 caattactcc tagtgatctt aattatgaaa ctactttatt agagactatg aattttactg
     4981 aattaaataa atatataaga aaacaaaaag aaagaggtgc agaaaatata aatgagcttt
     5041 tagtagaaag acaaaacaga tatgcatcac cattttcagc gattatatta actataattg
     5101 cggtaacatt atcaattaat aaaaaacagg gtggattagg tttaaattta gctataggac
     5161 tttcattaag cttcggatat attttatttc agcaaattag tgtttcttat ggtgttgcag
     5221 gtattttatc cccatttata gcagcttggt tgccaaattt tatttttatc attattgcta
     5281 tttatcttat ttatagagag atttaattat ctttttgtga taattatcaa tattaaaaat
     5341 ttagcttgat atacaaagta atgtataatt ttcttctagt gattttaaat agtgccaaca
     5401 tttttggttc tataacgttt tatatgggca aagatttaaa aaagagtaat tgtacaaaaa
     5461 agaatttgtt aatattgttt tattaaatag acatacgaga aagatggaat taaaagggtt
     5521 ataaatggca ctttgtgaac tttgtgtaat aacttagtgc cctttgtgat aaaaattagc
     5581 ctaaataatt aaaaatagtt attttttgta acaatctaaa attttattac ccacaataaa
     5641 cgttatagaa tcataattaa tgtatcaaat taataactcc tcgatatatt ttatcttttt
     5701 catttatagt acgtaatttt ataacatata aaaatgtatt tggattgata ttcctttcat
     5761 ttttgtcaac tatattacca tcccaaggtt tatttatatc tttgctatgg tacaataatt
     5821 tgccagtcct actaaaaata aatatctcgt aactatcgat atctattccc tgaataaatg
     5881 gagtaaaata atcatttaaa aaatcattat ttggagtaaa tgcattagga ataaatattc
     5941 gaaaattttc taaaacagtg atagaatcaa cacatataga tgtgcaatta tattcatctg
     6001 tagcatatag agttataaca aatgttccag actcattata tctatgagat ggattaatgt
     6061 cagtgctgct agtaccgtct ccaaattcaa ataaataaga aatagcaccg acagtataat
     6121 tataaaatac aattggttcg ccatccatat accataaata tgtgctagga gtaaatctag
     6181 cattaggacc atgccaataa ggaagtcgaa tagatgtgtc ttttgtacat agatcatctg
     6241 tgatggttac atgataatat ccgcttgata aattatataa ttcatttgtg tcaagattag
     6301 gattatgact ccagtaatat gtaagagggt gtaaattacc atttacattt ataatgatac
     6361 tgccatcttt attaccgcaa tgctctggta aaacattact agtaatatta aaaggtggca
     6421 aagtttctat aaaagtttgt ccagtagttt tgcaaccatt agcatctaca acagtaacaa
     6481 aataagtacc tccacataat tctgtaattt cttgagtagt ttgttgtggg taagtattcc
     6541 acaaatattg aaatggtggt gtaccagata aatgttctat cgtagcttgg ccattaaatg
     6601 catcgcaata tgcaggttga gtgttaatct caaaattcgg taaaggatta atggtaactg
     6661 taatgctagc tgaagcacta caactactac ccaaataacc cgttactata taagtagttg
     6721 tttgattggg ggttacaaag atactaggag tattcaatcc attactccaa tgatatgtat
     6781 cagcacctga aactgtaaga gtagcgctct cgccttcaca tatagcagta tcattaacta
     6841 tcagaacagg attactaaga actgttacta cagcaatagt gctacctgtg caaccgttat
     6901 tatcagtacc tgtaactgaa taagtagtcg tagtgctagg tgaaactaca atagaattac
     6961 cactttgttg attactccaa atatatgtac tagcgccctg agcatacaat gttactgtat
     7021 cgcctataca aatagtccga tcattaacta ttatcgttgg caaagggtat acgtttattt
     7081 gtatagtagc agaattttta catccattat tatctgtgcc agttacacta aaagaggttt
     7141 gttgattaat aattgaattt ataatattag tagtttcacc attagaccaa acataattca
     7201 ttccaccact agctattaga gttgtttctg aaccttggca tacattttga tcagtagctt
     7261 gtattgtaat agtaggtaaa ggatttacaa ctattgtagc agtagctgat gcagtgcatc
     7321 ctaaaaagct tattcctgta acagtatatg tagttgtaat agtaggatta tctgatattg
     7381 taaattggtt gctaccatta ttccactcat aacccatcgc atcgccactg acagagatat
     7441 agccagtttc tcctaaacaa atcgtatcat ttgtggtaat tatggtggga tttggagcta
     7501 acattacaaa tattgaagcc gttgcactac atccattagg tgcagtacca gtaacaaaaa
     7561 aaactgaagt actatctatc gatactatta tttgatcact tgtctctcca gtgctccata
     7621 tatactcggt tccaggcata tctgaattaa ttgtaatagt atctatgcct cctacatcac
     7681 aaaaaacaga agtagttaaa tgatatgtga gatttggatt gtcttttact attatagtta
     7741 cactagctga agctgtttca tctgaggaag atgtacctgt aacggcgtat gttgttgtag
     7801 atacaggtgc aacagtaata gatgaagtgt tatctcctgt tgaccattga aatgtggtaa
     7861 aaggtaaatt acttgtagca tttaaaatca aagaatctcc tctacaaatc gaagtatcat
     7921 taggtatgat atttacaatt aattgagctt ttaatgaatt tatatataaa aagcaaaaaa
     7981 taattatcaa ataagtaata aaaaagattt ttttcatttt ttattttatt attgtaatag
     8041 ttccattata tactttatca ggattatttg gtccagatcc agtatataaa atgtaataat
     8101 aagtaccccc tggctgatcc ttggcatccc aggtattatc atagccattt atttcataaa
     8161 ctacatttcc ccatctattt aaaatagtaa gagtagaata tgggtattta tcaatatttt
     8221 ctataataaa tgcatcattt ataccatctc cattaggagt tattacattt ggtatttgta
     8281 aaacatcatc tatcattaaa acagctctac taatagtatc tgaacatcca tattcattgt
     8341 aagcgattaa agtaactgtg taagtgccat cattttgata agaatgagaa ggaaaatcat
     8401 cagtagaggt agtagaatct ccaaaatccc atgatatgta agtatctcct aagctttgat
     8461 tataacattg tatggtaaag gtaggatcat ctgtagcata aactaaaact ggatcaatta
     8521 tcatattagc ttgtggactt ggatatactg tgataaactg agaataataa gctgtagtat
     8581 cacaaccaaa ttcattagtt acttgaaccc atatatctat attacctgca gtattaaatt
     8641 tatgtaaagg gttataaata gtatcaccga aactccataa atatcgatta gtaggaaaat
     8701 attcataatt agtttcataa aattgtacat atagtggttg gcagccaatg gtaggttctg
     8761 ccataatatt cacaggtggc aattcatata attctactaa atagatagta tcataggcta
     8821 cacaaccata agaatcaatt atttcaacaa tatagctacc tttaggtaaa ctatcaaaaa
     8881 tattagtagt actacttata ttatttgggt gtaaaacata agaatatggc ggtgtaccac
     8941 ctgatgctat tatcatgata gtaccggtag aatcatgttt acaccataca tctattttat
     9001 ctaagctaat attgataggt ggattactaa aaatatttat actatcactc acactacatc
     9061 cacctgcatc tgtaatagta acactatatg taccctcacc tactatgata gaatctattg
     9121 gtgtattagt aatattatta ttacctgttg accacagaaa attataaggt gggctaccat
     9181 tttgaacttg agcataaata ataccatttt gcataccttg gcacaaaatt gtatctccaa
     9241 taatatttaa ccctatggat ggggcagatg atagtaattc agcttggaaa attgaatcat
     9301 taaagcctag tatttcccaa gtatcattca tccacgttaa tgcaaaatca gtaatatgca
     9361 aagtatcatc tagaaatggt ccgaaatgaa aattgtaatg tgactttgca tcttttagat
     9421 tatttatatt attctctata tttactattt catttgcatt aatctctaca catagaggcg
     9481 gtacattaac catggcttcc ttagatacat taatatcaag atttaatgta aagcttaatc
     9541 caataatatt aaaattcaaa tcaatactat cccaaacatg gttattaaat tggtttttca
     9601 taaaatgtaa tattgggcct gtagacaaac tatctaatcc aaaacaaggc cataaaatat
     9661 ttaggttatt acccactata aaagtaacag tatcatttat cccactttgt atcacattat
     9721 tattagtatc tatttcttta tattctacgg gtgtaggaaa atgaaatgtg gtatatatag
     9781 taggacaaaa ggtcaaatct tgatttagat agtgttttgt aaataaatta gcttgatcta
     9841 ttaatgtata atttaattct atagtcatat caggatcatt atatatttgt atgcttccac
     9901 ttaatgagtt aataaaatta taaatagcgt cacctgtaga accgccaata atatctgcca
     9961 tagattctat aaattgaatt aaatttaatg ttaaattcat ataatagtct acaccactag
    10021 cttttaggca tttattagac aatactgtat cctcataaga tgatatatag ggtatatcag
    10081 cccataatgc tagacctatt cctccaatgt catttacttc taatggtata acattactat
    10141 agcaaatatc tggatagcca ccagaaatac atggatatgt agctatgcct gtatatgtat
    10201 ttaattccaa taatattact gaatcgtaag gtacattaat attaaaaaca ttataaggtg
    10261 tacaattaaa tgcacacaat tgtcctccca gattaatatt tgcactgaaa ttaaaatcca
    10321 taccgatcac accttcatct ggaaatgttg ggtgtaaata tgcattaggt aaaacattat
    10381 aatcagatga aatagtggcc cattgaccag gaattatggt gccataatca ggaaaagtaa
    10441 gagtaatatc tacaggataa ttaatatcca cccatccatt atgaaaaccg cttgcataat
    10501 atgataatgc tatatctaaa taagcattta aatttaaatc tatcccccat tgtcctaaag
    10561 ttcctagatc ttctatatat ccaaaacttt gattttcgtt aatattaaaa tgaaagatat
    10621 cttgatttaa atttaaattc caactactgc cttgtggccc ccacatatat tgatgattta
    10681 atgcccatgg tataggatga tgtacagtat atgtttgatt ataaatgaat aatggtagaa
    10741 aaataaaaaa aatgataaaa gcttttttca tatttatttc atatttaaat taatttttta
    10801 gttctaagga ccttaatgat tctagtaatg gagaataaaa aatctcatca atttctttag
    10861 ggtatgctgc agtaaatata acaatattat tactatctcc agtgaaaaag gttgttctaa
    10921 ctatttctat tatttgttca ttatgcaata cttctatgta accttttaat aaaataccag
    10981 ttttattgct gattgtgaaa tcaaaatttt ttaacattgg tattaattga ttatcaaaga
    11041 aaaatttttc ttgattaaat gtttctttaa aattataaaa atttctattt gagattagtt
    11101 ttatctgaat actaatgatc cctaatgtat cctgataatt ataaatattg tctgtaggtg
    11161 aatattttgt ctctactatt cctttaggag gataatatat tattgtttgt cctattaaaa
    11221 cttgagataa aaacaataat aaaattgtta atataaaatt atttttcatt agagttttat
    11281 ttattgcaaa tttatataat tttcttggaa ttttttttaa taataatttt tttattaaaa
    11341 aaattattta tctttgtata aaatttttgc ctatcgacta agacttttat gcttatatca
    11401 aaaaaattgt ataatatgaa agagtacatt gatatgagcg atgaagaatt agtaattgct
    11461 tataagcaag gagatcaaag aagttttcaa gaattatata agagatatca aagaaggatt
    11521 ttttcttaca ttttagtttc tgttaaaaac aaagaaatag ctaatgatat ttttcaagat
    11581 gtttttttta aagtaataaa tactataaaa tctggtaatt acagggagga gggcaaattt
    11641 gctcaatgga ttattcggat aactcataat gcagtggtag atcatttcag atatcaaaat
    11701 aaatatgcta tatctaataa taattgtgat gataatgaaa attatttctt cgatagcatt
    11761 actaaagatc attctatttc accagaagaa ttattaataa aaaaagaaaa taaatatttg
    11821 gtacggcaag cagtatctac actttctgat aaacaaaaac aagtagtata tttaaggatg
    11881 aatgaagata aatgttttag agaaattgca gaggctcaaa acattagtat caatacagct
    11941 ttaggcagaa tgagatatgc tattatgaat attaagaaaa aaattgatcc ttttttataa
    12001 tttttttaat aataaactca taggtattta cgttattaaa taaaaattgc ctatgaatga
    12061 taattatact ttaaaaaatc gaaaaaaaat tttaaaacat ttgagaaaat tgcatcttat
    12121 tgtaatttct aaaccaaaaa aagaagtaag taacttaata atgcaatata gtcatctttg
    12181 tatgttaaat aaagattttg ttatattagc taattaagaa atcaactttt tttattttct
    12241 ctcttttgat gaagatccga atgatataag acttagatta atatatcgtt tgggattttt
    12301 acgtaagtct tctaataaat aattaagact ttgagagctt tttgttagat aaaaatataa
    12361 actatcatcg tatatcaatt tgccaatagt gccagatttc atattaatag tatctataat
    12421 cttttgaagt tcatagatat ctttattcaa attatttatc accaaatgca aatcactatt
    12481 ttttatcgta tctgttatct gatttatatt tgtaatgatt tgatttattt tatcattatt
    12541 agcttttata ttagagctta tattttcaat gttttctaga gatttattta aactaattgt
    12601 agtttttttt atattttcta ttgtcattat caaatcattt tgaacttgtg gattagcgat
    12661 attttgaaaa attgatatag tactatctat tgaggccata agggtaatgg ccttttcttt
    12721 taaaggtgag agttcggctc ctagtatttg agtaaaatct ggactcactt ctccttttaa
    12781 aaaatcatta tcttttatat attctttagc atcacccaag attattttta ttactttacc
    12841 ttctaaaaaa tctccacttg ttattaatgc aattgaattc tttgggattt ctatattggt
    12901 ttgaatgagt aatcgcactc gaaccttgta atcatcagat ataaatttaa attcatctac
    12961 tttaccaata ttgaggccat tcaaaagtac agggctatta gtagtcaaac cataagtctc
    13021 atcataatct acgtaaacaa tagtattgtc agaaaatatt tttaaacctt ttaaaaagtt
    13081 gatcccccaa tataagaaac ctatagaagc tataaatata acagctatca atattttttt
    13141 agttttattc ataaaaattt tttagcaaaa ttgcacaatt tttttcaatt gatttagaaa
    13201 tatacaaaaa tatttagtat ttaggtgttt tatttttatg aattcttttt tcaggattat
    13261 aattatcttg atcatctgga gcattacgtg catctatgtt tgttctatag acccatctat
    13321 tgtctattaa ttctaaaccc actaaatctt cggcatttgg aaaataatat tgatattgtt
    13381 catctaaaaa tttattgagt ggtgataaag tattgaatac tatcatattt ctattatatc
    13441 ttttagtctt tacaattttt ttatctcttt tttgtgcttt tttaaaattt ctagatcgat
    13501 taggtttatt tctttttttt atttttactg tatttatttc ataagattgc ttttcgtatc
    13561 tcagagtcat tacagcttca tcgctatatc tgaaaattaa ccgagtagga ttttttttgt
    13621 ctggtatatt tctaaatatt cttttaccaa aaataggcag accattatac gaaaaactca
    13681 atacatctat gagttttgat tgatacaatt cattctcacc atcccaacct aataatgtgt
    13741 aacatttaat attattaatt tctaaaggta taatttgata atatattgct ccccaccaat
    13801 ctccattttt taatattgct cgttcaggat ctgataacga atcttttata ttttttaatt
    13861 ctgttaatct ataagtatca tcttttgaca atttttttaa agtaaaccca taaggaatag
    13921 ttttgcctgt agatagtgat actccccaag taataattaa aaaatcatcg cctctgagag
    13981 tatataatat ttttaaagta tcaaaatcat atctataggc atctggatag cttagtattt
    14041 ctgagattaa gcgaaaaaaa tattcatttg attgtaattt tataaaatca ttttttcctt
    14101 ctattattaa attttcatat aataataagc tatctaggtg cggtggataa acaagagtag
    14161 atttttgccc taataaaaaa ttaaaaatta aaatgtttat aaataagcat aattgcttca
    14221 taattataat aacttaataa ccgattagtt catatccttt taattgttac ataattttta
    14281 aaaaggtgaa ttaaaatcat caaataatat gccatttcta tctttatcgg ataatattgg
    14341 agctttacaa ttccacggaa tattgagggt gggatcgtcc cacgctatag ttttttcaca
    14401 ttctttgctg taataatctg agcatttata tagtaatatc gtttcatcag ctaatgaaat
    14461 aaaaccatga gcaaaaccta taggaatata aaacgattgt ttttcagttt cattcaattc
    14521 tatcattaga tatttgccat aggtagggct attttttcgt aaatccacag ctacatctat
    14581 ggctttgccc ttcactaccc ttactagttt ggcctgtgga tgaggtggca cttggaaatg
    14641 tagacctcta atgacattac gaatagatag tgactcattc tcttgaacaa aatcatctgt
    14701 tatctctgct tcattgagat gactttttct ttgagtttct aaaaaataac ctctattatc
    14761 tttataaata acgggtttga ataaaattaa tccttctatt tctaaattaa ttgcttccat
    14821 attatttatt ttttcttaga aaaataattt aaatattttt tcatagattt tgattgtttt
    14881 gtttctttca ctccatatcc atcatagtac ccatagatat cgtatttgta ctcataacca
    14941 taattgctat attttgcttt tctaatatat ttttcattga aaccatttaa tacaagtatc
    15001 agatttttag gatttaattc ttgttttaat ttttttacat tatacaaaaa ttctttttta
    15061 gaaaaatcac ttctaaaaac ataaaatgta gtgtcagcta acataataaa tttggtagca
    15121 tccgatataa ggcctattgg aggcgtgtct agtataacta tatcatagga ttgggtaagc
    15181 tcttctatta atttatccat tttgttacta gtcaatagct cattaggata ttctggtaag
    15241 ggtcctgctg ggaaataaaa tagattacta agctctgtat ggcaaataca ttgttctatg
    15301 ctacattcac cttgcaaata gctagatagt cccaattctg atttattatt aaatccaatg
    15361 ctcattttag gccttctcag gtcagtgtct atgatcaata cttttttgtt agataaagct
    15421 agaatgccac ccagattaat agctataaag gttttgccct ctcctggtat agtacttgta
    15481 attactatta ctttaggtat gttatcttga tttattagca aaagatgact tttgatcttt
    15541 ctaaatgatt ctgttagaag tgatctttta tacttgtgta taataagctg agaatctgga
    15601 atatcaccat taaataatgg caaagacaat atgggagtat cagggaatag tgattctata
    15661 tcttggatag acagtatatt atcatatttt atatatttcc atattaaagt agaagttgag
    15721 atcaaaataa atataaataa tcctattatt aatgatttag ttttgttagg gtaaattgga
    15781 ttagaaagta gagttggttc ttctaaaacg accagatctg atactatacc agctacatag
    15841 agggtatatt ccaatttttt atttagaaat tgttcataat attttaggct tgactgagat
    15901 atatcagtta agggttccat tgttgttata cctgctttct tttcaatatt gcttagctta
    15961 gatttataat tttgcaattg ttttttcaat gatgataatt gaaaattaga tctttctatt
    16021 agtgtttcta aaatatcatt taatgaattt ttgctataat gtattttcca ttttaattgt
    16081 tctaatgtat atgtattggt agaatatata gcagatagag ccagatactg ttctaataat
    16141 gagcgatact cgtttacaaa tgttgataaa atattgtaat aactagtttg agataaatat
    16201 actattaatc tagatgtatt caacgaatct ttattcaata tctcttgtga tttattcaaa
    16261 attactatct catcattgat gcgctctatc tccaactcta atttatttat ttcttgcttt
    16321 aaagaaaaaa acgaagtgaa agtatcattt gtagataaag ctatattatc attaccgctc
    16381 tgttgtttga taatatcttc tagagattct aattgctgat ctatatattc tagcatgctt
    16441 ctagcactca tagtgcgttg ttctacatca aaaaatttgt actcttgtat tacagtattt
    16501 aaaaaatcca cacattttaa aggattttga tcactaaaag acaactctat tgatccagca
    16561 ttaggattta gaatatttgt agataattga taacgatatt gcttgattaa acttggtaaa
    16621 tcatgaataa taatgcaata atcgccttct ttaatttcat ctttgggtat taaaacaaac
    16681 tttatatggt ctaatataat agtatcataa aatgtaccct gagtagcata agtgttatta
    16741 ttaattttat aaaagatatc tatagtatta tcatttttga tttctatatt aatcagttta
    16801 tttattatta agctgtcaat tatctcataa gtaaatagaa agggagcaga gcgatacaac
    16861 tcgaaatcta atattttgcc tttagcatat atacttgtgt ttagaggtaa ttgttttatt
    16921 actcttttta taaattctgg tgatgagagt acttctacac tagtgtatat attatcttta
    16981 ttcaatgcac ttgagcttat tacttctgat gctctatttt caatgtttaa ttgtattact
    17041 gctttagctt ggtatacagg ttttgtatat ctaatagatg caaaaacaat aaataatgtt
    17101 acaaataaat acaatagcaa aagccatagg ttctttttaa aaactatttt aaaaatatat
    17161 gggttaaatt cattattata tttaggaatt ttaggttttt cgtccattta ttataaataa
    17221 atcaatattt ttacaaaaat atgaattttt taagatttaa ataaaaatca atatttttct
    17281 attataaaaa aaaaaaaata cttacttttg cataaaatat tttttgtgca taatcaagct
    17341 attgtaatca tacccactta taatgaaaaa gaaaatatag agtctatcat aagaacagta
    17401 ttttctttgc ccgaaccctt tgatattctt gtagtagatg ataattctcc agatgggacc
    17461 tcagacatag ttaaaagtct aatagatgaa tttcccgata atctaaatct attattaaga
    17521 gaaaaaaaag agggtctagg gcgtgcatat attgctggtt tcaaatgggc attagagcat
    17581 aattatgaat atattctaga aatggatgcc gatttttcgc ataatcccga agatctcgtt
    17641 aggctgttag ctacctgtag agatgataat tatgatttat ctataggttc gagatatgtc
    17701 tcgggtatta cagtgatgaa ttggcctttg tctcgagtat tattatctta ttttgcttct
    17761 aaatacgtaa atatcattac tggtatgcct gtaaaagatt ctacagcagg gtttgtatgt
    17821 tataaagcat catttttatc ttctattgat ctcgataaaa tatcattcat tggttatgca
    17881 tttcagatag aaatgaaata ttatgcttat aaattaggat ttaaaatcaa agaaatacct
    17941 attatatttt ccgaaagaca gcatggacaa tccaaaatga ataaaaaaat agttttcgaa
    18001 gctatgtggg gtgtcataca aatgcgtttt cgcaatgtaa aaaaactttt aaaaaaataa
    18061 tggatcgatt tcatatttat aatgtaaaca ttattaatga aggtaaaata tttaatggtg
    18121 aaattttcat ttctaatgga ttaattgaaa aaataaatca taattctagt agctcgactc
    18181 ctacttctta tcaaaaaatt gatggacaag gcaattatct cattccaggt atcatagatt
    18241 ctcatgtgca ctttagagag cctggatata tagataaggc taccatagct tcagaatctc
    18301 tagcagctct ctatggtggc gttacttcat ttattgatat gccaaataca ttaccaccta
    18361 ctactaataa gaaaacactc gagcataaac ttattattgc tcgtgataaa tcttttatta
    18421 attatggatt ttttttaact gctgttgaaa ataatatttt agatttacaa cagataaatc
    18481 ctactcaaat agcaggtatt aaaatttttt ggggtgatac tactggtggt ttgaatctga
    18541 tagataccga aattattaat gaagttttta aacttccttt tttgatagct gtacattccg
    18601 aagataatgc tactattcag tataatgagt ctttgtatct aggttcattc aaaaatgatg
    18661 tctacataca tcctattatt cgtgggttgg atgcatgtat tatcgcagct aaagatatct
    18721 tagggctagc tagtaaatat cagactcgtt tgcattattt gcatgtttcg catattaatg
    18781 aattaaactt tattttacaa tggagagatc aaataaaaca tttatctata gaaacttgcc
    18841 cccattattt gtatttcgat ttatcacatt atgatgtttt tggtaatttg attaaagtaa
    18901 atccctctat caaatctacg ccagagcaac gaatacaatt gttaaattca ttaaatataa
    18961 tagatactat tgctagcgat cacgcaccac atctaaaaca tgaaaaacaa aaaaattatg
    19021 atttagctcc ttcgggactc ccttctatac agcataactt gggattaatg tggaatctcg
    19081 taaatcaagg atatcttagt ttgccagatc taattgaaaa aatgactcat aatccagcta
    19141 agatttttaa aattaaaaat agaggttata ttcgcgaagg ttattatgca gatttagttt
    19201 tggtggatga caaggtaaaa gaaacaatta gtactgaaaa attgaaatat aagtgtggat
    19261 ggagtccata cgaaaatttt acattgccta tctctattaa aaaagtttgg gtaaacggta
    19321 atttaatatt agatgatggc aaactaaatg acattaaatc tgctcaggca ttgatttttg
    19381 aaaattatta attaaattaa tcaaaatatt ttcttgtaaa gtttcatcca ttatttcttt
    19441 tatatctttt ttaattattt tttaatattt tattatgtaa ttttgcgaat aaataaaaat
    19501 tttgatttta tgaaatattt taatttttct ttaatgttct tttttctgat aaatgcaagc
    19561 atattcagtc aaaattattt ttatgaagat ttttcggatg gcgattttgt atctaatcct
    19621 gaatggactg gtgatgtgga caaatatatt atttatactg gtacagcagt gccagcaaat
    19681 atgaagcctt cattacgtac taatggttcg gctacagaca ctacttatct ggcaactgaa
    19741 tttgttcaat cttttacaga tagcttagag tggaattttt gggtaaaatt aagtttcaat
    19801 ccatcatcag gaaattatgc tcgtatttat ttatgttctg ataatgaaaa tctaaaagga
    19861 tcactaaatg gatattatgt agctatagga tataatggta atgacagagt aactctagta
    19921 aagcagcagg gaactaatca tacggcattg atagtaggca gtagcgcaga tttaaataaa
    19981 agtacaaata ttttacgtat tagagtaaaa cgctatgatg atggtacttg gttgatgtac
    20041 tcagatgaga gtggcgggtc caattataca ttagaggggc aattcactga tgctactttt
    20101 acctcctcta actatttagg tgttttcaat caatatactt catctaatgc tactagtttt
    20161 tattttgatg aattctatgc tggtcctatt attatagata ctatacctcc caaagtgata
    20221 tctgctaaag taattaatag ttttgattta gctattacgt tttctgagac tatggataat
    20281 agtatatatg atagtgataa ttactatgct gatcatgggc tcggaatacc aacttctgta
    20341 ttagatgatc ctaatagtag taatacagta catttatctt ggatgccaaa tccaataata
    20401 gatgatacta tttatcatct aagcataaat aatgtgtcag atttggctgg aaatacatta
    20461 gatacgacta ttatttttac atttcatatt ccagtagctt atgatgtttt aataaatgaa
    20521 ataatggcag atccagatcc acctatagct ctaccacctt atgaatatat tgaattatat
    20581 aatcctaatc attttcctat taatttggag aattggaaaa ttaggataag taacactaca
    20641 aggattatcg gtaacgtaga tatagatcct aatggatact tgatactttg tcatcaggat
    20701 ggtatgcaag atttcgaaaa tctaccacaa cattgtaaga ctttcggatt ctcatctttt
    20761 tctattacta atgcgggggc agatattgtt ttgtattcac ctgatggtaa tgtgatttct
    20821 tttgcagctt tcagagatac atggtatcaa gatcctctga aagctgaggg tggttacagt
    20881 gtagaaatga tagatccaaa caatccttgt gctgatgaga ctaactggag agctactaat
    20941 tcattatttg gtggtactcc aggaattata aattctatac atggctctaa tcctgataat
    21001 gataatccag aaattttaag agctattctg caaaattcta caactctatt agtattatat
    21061 accgaaccac ttgatagtat ttatgctcta gatagttcta attatataat cgaaccacta
    21121 ttaaccatca ataatataaa gggcaatgaa cctttttata atgaaatcac aataatattt
    21181 aatgaatctg ttgatagctc agaaatatat actatatatt tcaaagattc tataagagat
    21241 tgtgtgggta atatgttacc ccatgagacc tcagctcgtt tcgcttttgg taatatacca
    21301 gaaccctacg atatagtgat aaatgagata cttcatgacc ctatcggaaa taatacaccc
    21361 tttgtagaaa tttataacag aagtaataaa attttagatc tcaaatattg tgtacttgct
    21421 aatagggatt ccttgtatga gctagcaaat aaaaaatcaa tagctcctgg tggttttccc
    21481 atttttccag aagaatatat tgtattatcc aaaaataaaa aacaaatttt atcgcaatat
    21541 tatactccaa accctaaaaa ttttattgat atgaatttgc ctaaaataac taaagattct
    21601 aatactattg ttttagcttt gactaatgat accataatag atttagtaaa atattctatt
    21661 aaatatcacg atcctcttct cataagtact gatggagtat cgctggagag agttaatcct
    21721 aatagaccta gtaatgagtc tagtaattgg catagtgctg ctgctactgt cggttttgct
    21781 actcctgcat acaaaaatag tcaatattca gatgatttat ttactgatga ggttactata
    21841 gatccaccat atttttcgcc cgacaatgat ggatttcaag atttattaga tatttgtttc
    21901 gatttcaaag atccaggtta tcatgttagt attacagtgc ataatttagc aggacagcca
    21961 gtgagacatt tattaaggca ggaatatgtc ggtactcata tgtgctatag ctgggatggg
    22021 agggatgata atgataaacg ctgtgagttt ggttggtata taatacgtat ttccgctttt
    22081 aatacaaaag gagataaaaa agaattcaaa aaagcagtgt cattaaatat tagaaaatga
    22141 tgttttagag taaaaaaata acttttatcc ttcttttaaa tctaatcaaa caatgagcat
    22201 cttatttgtt taataaatat agttttgtgt attttgatga taatgataaa tagttaataa
    22261 tgtactttga ttctgaaaaa ctgaaattat tattttacca ggttgataaa tatttccaaa
    22321 tcttgtcatt attttgatta tttattagat agttatattt actcttttgc aagtttaact
    22381 caaatttata tagctgatct cttatagtat ttaattcatc tattggtatt tgctcccatt
    22441 tgtcatattt tatctcttta ttaaaattaa ttttaataaa ttttttaaaa tgctcaaaat
    22501 ctttttctgg atcaactttg taatttttat ttaaatattt tattatatga aaggcatcaa
    22561 agtattcgtg tattgcatga ataaatctat gttttttggg attattattg tgaattttag
    22621 taattatttc tctaggaatt attttttcta agtgatttac aatgtcatct atcatatcaa
    22681 agtgttctat gctttgatat atgtctctga tggcttcaaa taattgggtt ggataaaaag
    22741 gatatttttt actctgctct cttagagtca aatttatagc aggacctgta ccgaaaacaa
    22801 ccctatctga acttctagca gatggatata cattagtatc tgaccagcca cttaccatgc
    22861 ctatttttct aattttttgc atagtataaa aatcctcacc agcatctctg attttaaatc
    22921 taccagactt tttgtaagct ctaacagtga agctaatagc tgagccaata ggcatgtatg
    22981 catatgggct cttgatcaaa agtaattgaa tagtataata tctcaaaaaa atctcatatc
    23041 ttagcattaa taaagcttga ttactggata aattattgat tttatgataa tatggtgcac
    23101 aaatgccttg taataaaggt aattgtttgt aaatgttgta taaactattt aggtaattgc
    23161 tagggtaata tgtgtcagca tctatattta caatgatatc atcatcattt gctaaaggta
    23221 ataaagcatc aatgcagagt ttgcgtgcaa gacctactga aggaatcttg gatgcaaatc
    23281 ctttaccagt agaggcatga tcgagtataa taagtttaat cgggtatgag gtggatattt
    23341 gatttaggta atttagagtt ttgatattgt tacagacaat ttctttttta tcaggcgagt
    23401 cagaccaatg tttgggttga tttacacata aagcaataac tttagcacgt agtttttgat
    23461 ctgccaagca ctccattaat gtagaaatat tttcaaactc attcattaaa ggaatagcta
    23521 ccaatatatt tttgtcatta ggatttaaca ttattatttt gttaattata aaattaataa
    23581 aattttggat taaccaaaga tatacatttc acatataaat aacttcattt actcgaaatt
    23641 aaattatacc tttagttagt gatgcttagc aagatttcga ttgcctaata taaataaaaa
    23701 ctcaatagag attggatata cttttattaa tatatttata ttaagttatt gattacaagt
    23761 atattttaat aattttaata atagggattt gcgaagtttt ttacatcttc taagcttact
    23821 ttttcattgc aaagtatcat taatctctcg acgacatttc ttagttctct tacattgcca
    23881 cgccagggca aatttataag atactcaata gcattctcat caaatttgat tgtgggtttt
    23941 ttaattgatt cgctgagttc atttacgaaa tggtctatta gtaatggtat atcttctttt
    24001 ctttctctca gaggtggcac ttctagtata ataacagcaa gtctatgaaa taaatcttct
    24061 ctaaatcttt tatttttgat ttcttctacc aaatttttat tagtagctgc tattactctt
    24121 acatctacgg gtatatcttt ttcaccacct acgcgagtta ttttattttc ttcaagagta
    24181 cgcaatacct ttgcttgtgc tgccaagctc atatcaccta tttcatctaa aaacaaagta
    24241 ccattattag ctagttcgaa gctacctttt ttagatttaa tagcagaagt aaaagcacct
    24301 ttttcgtgtc cgaaaagctc actttcgatt agttctgatg gaatagctgc acaattaacc
    24361 tctacaaatg gaccttttgc tctaggactc aactcatgta accaatgagc tatattttct
    24421 ttaccagtac cattttcccc cattattagt actctagcgt cagtaggtgc tatacgttct
    24481 atattttctt ttagcttttt cattgcaggc gaatcaccta ttaatgcata tttttttgat
    24541 aatttatttt ttagtctttt agtttcttcc atcaaatttg atctatctaa agcattgcga
    24601 atagatataa gcaatctatt aagatcaaaa ggttttatta taaaatcgaa agctcctttt
    24661 ttgatagcat ctactgctgt ttctattgta ccatgccccg atatcattat tactggtata
    24721 tcataatttt ctaatatttt ttccaatagt tccataccat ccatgcctgg catcttgatg
    24781 tcacaaagta ttacatcaaa agattgagaa cttaataagt ctaaagcttt agttgcattc
    24841 tcagcgtcta ttacttcata attttcatat tctagcattt ctcttaatgt ctctcttaca
    24901 gacttttcat cgtctactat taatatgcga ctcatgttta ttaaattttt aaataaaaat
    24961 tgaagtataa aattaactat ttaatttttt gtaataattt atctctttaa atttttaaaa
    25021 aaaatcagag catatttatc aaatacgctc tgattttttt aataaaagat tataaataat
    25081 tatctgtttt tagatccata attcactgga gctcccacac gagtcatagc acatctaaac
    25141 cctatgtaat cagtagccat ctcttcatct aaaaatctac gagtaccagg actaagaaaa
    25201 taagcacgat ctttccaaga accaccttta taaactctag cctgattatt tattaaagaa
    25261 gtttttccat attcatacat gattttattt cgttgatctt cttctaaata atcagcctga
    25321 tcgtaataaa tagagctagt gtaatctcca tctaaatagt ctctgttatc agcataacgg
    25381 aaattgtatc tatttttagc ttcttcttct gtcagaggtc tccattgtat tcttcctaag
    25441 gaatcttttt ctgctatcag accttcttcg tctcttactt ttgtcataaa tacattacct
    25501 ctataaggtc tccattcatc catatcttca taagataaag gtctatacac atctaatacc
    25561 cattcagcca cattgccagc catattatag agtccgaaat catttggcca atagctaaaa
    25621 acaggagctg tagcatcata tccatcattt agataaccgg ctacgcccat ataatcacct
    25681 ctaccgcgta caaaattagc tacaaaatct cccatatttt tatgatagtc agtacgtaaa
    25741 acatgtccat tccaaggata aactcttcgt tctaaaacgc gttcataaac agtattgcct
    25801 attaagccta gcgcagcata ttcccattca gcttcggtag gtaatctgta tttaggtaat
    25861 aaaatgccat cttctacacg tacttttcga gttcccatgc cagtagggtt caaatcatat
    25921 aattcatttt ttactatacc ttcatattgt ccagctagat atgcatcagt attgaagttg
    25981 ttttcattca tctgcatatt atctagtttt aaaattcctt cacgtactag aatatattca
    26041 ttcactctat ctgtacgcca cgaagcataa tcatttgctt gacgccaatt aacacctact
    26101 acagggtaga agttgtatgc tggatgacga aaataatatt ctacgaatgg atcgttatat
    26161 cctaatcgag ataaccatac caacgtatcc ggcaaagctt tattaacaac ttcagggtaa
    26221 tctgtgccaa atactcttga tagccaatat atatattcac gatagtctac attagctact
    26281 tcggccatat ccatataaaa agatgatacg gagacccttc taggaacatt gtcccattga
    26341 taagtaacat cttgttcagt acgacccatt acaaaggtac cacctgtaac tagaactaag
    26401 ccaggaccag tttcttgttc ttcataagga gctacttcga agccacccca attgggatca
    26461 ttatataccc atccagtagt acgtgatttt tcttgagcac aagcacttat aataaatata
    26521 aaaactaaac ttaaagtgct aatttttaaa aaactcttta tattcataat ttatataatt
    26581 tttcaattta atttaaaatt ttggacaatt aataggagca atttttttag ctttaacagg
    26641 acaaggcaat ctgaatgtta aagaaaattc atgagcaccg ccagactgat tagtcaaacg
    26701 tgaaacagta atatcataac tataacctat ctgaaataat tcttgaataa aacctactaa
    26761 aactatgata gcatcgggat ttttagtact atgtctaaac caagtgccga caacaaaagg
    26821 gtatttattt aagtataaac cgtaattaaa ctcttcgaaa tcttgttgtt tagaaaataa
    26881 aatattagga gacaaggttg tttcctctat agtcaatttt ttataaggtt tagattctat
    26941 actaataatg ccaccagcgt gcaaagtaaa tctaacaggt aaataagaag tactagtaaa
    27001 tccttcgttt ggagtggtaa catgactagc tgaaaaacca aaataataac gatccccata
    27061 accagctata ccagcactaa agtcagcaaa attttttgtt aaattttctg gtgggatttc
    27121 atttgtatta tgtataatac cccacctagg atctatttga tcggggaaaa ttaatttttc
    27181 ccaatctaga gatttctgta caaaagtagc ttgtagagct gtatttatat aaaaattatc
    27241 tgaaactttt aatctaaatg catacatgaa accggccata gtagtattta atataccctc
    27301 accagctcga tctacattta ctaaaagccc tatgccgcca gataaagccg gtatatacat
    27361 atcataagat gcattgtatg taacataagt accagtaata cttggccatt gatttctaaa
    27421 attaaaagct aaacgtggac atatctgagc acctgctaat gctggattta gatataatgg
    27481 attagcataa tactgagaga aatgaggatc ttgacccttt aaaattgtta tgcttccaat
    27541 taataatatt aaaataaaag tgtatttctt tttcattatt tttttatttg ctacaaaaat
    27601 aatatatttt tattaaacaa aagtaataat tttttattta ttgaaaaata atttcattta
    27661 acttgttatt tatacataaa aatttataat taggttacat tataattatt tactgtgata
    27721 ctaatatttc gttatattta gatgcattca taggatctat tgattttaaa atatttacta
    27781 tttctgtttt ttccatagtc ggtgcaggtt tgaaaatgtt tactaattca tcacttttgg
    27841 cagtcataaa taaatttata ataaataatc ctggtttttc tttatatgct tgttggatca
    27901 atttcattgc ttgaataata ttgcttctac cttttacaat atcatcaacc attatgtcta
    27961 atccgtgtct atgatatagg taaaaacctt ttctataatt atcataactt ttattagtta
    28021 aattttctaa aatccaatat ctatttcttt gattttcata agaacgccat cccttgtatt
    28081 gtgtagcatt agcaggaata gaattaataa ctgaccatgc tttttcgtaa aagggatttc
    28141 cgcctaattc ctgaaaacta tcaaaatcta aaccgataaa cagatatgca taatatgcta
    28201 atagagattc taaattaccg acaaaagtat tttctatata gtctaatgat gtacctaaag
    28261 tatattgaaa ttgaatgtct ttgtcttgaa aattaaacaa agtagaatga taggatgtgt
    28321 tatacactgg acgcctgagt tgtacctgaa aagtaccgcc atacatattt acgttatcat
    28381 aactattaat agtgattata atcgaacatt ctatgcgctc ctctggctca aaattgtaat
    28441 tagtccattt gcgagcattc ataaattcgt agatagagct ttgcaaatta tcaaaaattg
    28501 attgatctac gttagtaata gcactcgaat taatagagat attgcaattt aattcttgag
    28561 aattaacaga tacattttga tgtaatatta gtaaaattaa aaaaatacaa aaaatttttt
    28621 tcataatttt tttatttcat ttacatagat ctactataac attatcaata atatatttag
    28681 ctatttgttt ttttggtaat ttatcagtat tcaatttttt tccatttttt gtaaaaatag
    28741 taattttatt aaaatcactt cccataccta tgtctgtagt ttttgcgcta ttcaaaataa
    28801 tagcatctag atttttattt tttagtttat ttatagcatt tttctcttca ttttcagttt
    28861 ccagagcaaa gccaactatc cattgatgat tttttttgtt acttaactcg agtaaaatat
    28921 caggagtttt ttttaattct aaatttaaaa aatcagattt tttaattttt tccgtagcta
    28981 tgttgatagg ataataatct gatactgcgg cattcataat cacgccatcg acatggtcaa
    29041 aatatttatt gcactgatct agcatttcat tagtagtaaa tacgttgata cgcgtaactt
    29101 ttggatgtga aatagagaga ttagtaggtc cagcgatgag tattacctct gctcccattt
    29161 ccgctagagt ttctgctata gcgaagccca tttttccact acttttattt ccaataaatc
    29221 tgacaggatc tattggttcg taagttggtc ctgctgtaac caagattttt tttcccaata
    29281 aaactgattg agatcccaaa aaggcattta tctcatctac tatttgctgt ggttccatca
    29341 tacggccagt tcctttatag ccacatgcta attcacctgt atcaggattt aaaattttta
    29401 taccgatatt ttttaaaatt ttcaaatttt gctgtaaaat tggatgttgc cacattttca
    29461 cattcatagc aggggctatg aatacttgct tatcaaaaga caaaaaagtt gttgacaatg
    29521 catcatcagc tatgccatga gccattttgc caataatatt agttgtggct ggagctataa
    29581 gaaatatatc agcccaatct gttaagctaa tgtgttgagt accagtgaat tttgcattat
    29641 caaacatatc agtataaaca tcataattag ttaaagtcct aatagttgta ggtgtgacaa
    29701 attgtagagc atttttagta gctactactc gtacttcagc attattttgc aacaaaactc
    29761 taataagcgt aggaatttta tacgctgcta tactgccact aatacctata agtatatgtt
    29821 tgtcttgtaa atacatttat cagtgattat tgatgctctg gtattcgaaa atatattttt
    29881 ttatttaaaa attccttagt agctatcaat gtaggctttg gcttcatttc atactcacgt
    29941 gctacttcta tctgagcttt attttctata tattcctcta atgtatccac agtagtttta
    30001 aattctgata atctctcatc gagttcttct tttgtctgtt ggctaatttg atttgccctt
    30061 ttcccaatta ttaaagtact caagtaaata ttaccagtga tttcacttat ttgtttgata
    30121 tctctggtta cggcttcttg ccatatccta tgatcttttt ttttcataat atattttgtt
    30181 ttgattcaat ttgtttttct atttcggctt ttattagatt taatttattt gcatattgac
    30241 tatctggata taacatagta aaattattaa ttacgtctat agcttctaaa aatctaggta
    30301 attgcttgtc tgccacacta tttaaagcat aataaaaatg agataaacac aaataaaact
    30361 gcgcttcttc tttaaattta gaagcaggat aatcttttat aaaattattt aatgaaacta
    30421 ttgcagcttt atattccttt atatcataat ataatttaga atttgtaaaa tctttttttt
    30481 cgagattagc agtcaattta tctatgcatt ccttagcatc ttttactttt acgctattag
    30541 gatatctagt gataaaaagt tgaaaatctt ttatggctcc ctttgtaatg gattgatcga
    30601 gatagtatgg tggtgctagc atatatttac aatatgcagt ttgaaatgca cattcctcag
    30661 catatttact cgaaggaaat gtcctagcaa actctttgaa atgataacta gccattatat
    30721 aatcaccttg ataataataa ctccatgcca tatgatagta taattcttgc gatttagtgg
    30781 taccacgata ataaggtatt atcatttcga ttaattgtat agctttataa taactgcctt
    30841 gttgataata ttccatagct ttagcatatt tgagttctgg atcatcagct tttacaattt
    30901 tattaaactc aacattttct ttacaagaag ttaatgttat aattaatata aaaaataagc
    30961 tatattttaa cttcatctta atattaaaat cagaataagt aacgaattat atagaaaaaa
    31021 attgcaaatt ttattattat ttttttttta aaaaaactta aattattcaa tgtttgtcgc
    31081 cctcatgatc atgcgaacaa gctttttcta ttttagtgtc tttggggctt ttttcggtta
    31141 agtatcccac tttgtggatt agatatatca gatcagtttc tttatacctt tcaggattat
    31201 atacaatagt tactattttt gtttctaaat cagcatttac agaatatatg tcttcccttt
    31261 tagataattg gtcttcaatt aattttttcc cattttcgca atgaaaataa gtttttactt
    31321 taaactctgc ttttttcgta gggctgtttt gacttggtac ggatacttgt gcatttacaa
    31381 atacaggaat agtaaatgct aatactacaa ttaaaaataa ctttttcata atttttaaaa
    31441 tattttaatg caaaattaca aatttttttt taaattaaat taactttctt aaattattat
    31501 aattttttgt aaacttattt tagaatgaga cagatgtgaa ggaaaaataa ggagtagggt
    31561 gggagcgggt aagtttatta gatagtatgt ttttttgtaa aaatatattt tgtatgatgt
    31621 atattttctt gtaattttgt tttttattat ctaatcaata tggacgaaat tatttattta
    31681 aaaggcattg aaaaacatta taatattggt caaatcaaag taaaagcatt acaaggaata
    31741 tatttaacta ttagtaaaaa tgaatttatt gttattatgg gggcttctgg ttcaggaaaa
    31801 actactttaa tgaatataat aggttgcttg gatacaccta ctagtggtga gtattacctt
    31861 aataatcagc tcgttagtgg tcttgatgat gatgaattgg caaatattag aaataaagaa
    31921 gttggcttcg tttttcagac attttatttg ttacctcgct ataatgcttt agaaaatgtg
    31981 atgttgccac tcgtatatgc cggagtacct aaaaaagaaa gaattgaacg agcagtggct
    32041 atgctagatt tagtgggctt aaaagacaga atgtaccata aacctaatga actatcaggc
    32101 ggtcagagac aacgcgtagc tatagctaga gcactaataa ataatccttc tatacttttg
    32161 gctgatgagc ctacaggtaa tctagatagt tattcttctt atgaaatcat gtcgttattt
    32221 gagaaattgt acaatgatgg caatactatt gtgctagtta ctcatgaacc agatatcgct
    32281 gcttttggtc atagaatagt gaaattgaga gatggtaaaa ttattagtga tcaagctaat
    32341 ttaaatatag ctaatgctga atatttttta aaaaattacc aagaaatatg aaaaatcaag
    32401 aaaaaattta tactaaatat ggtgataaag gatatactca acttttatct ggtgaaaaag
    32461 ttccaaaata taatcttcga atagaagctt atggtaatat agacgaagcg actgctttta
    32521 ttggtttttt atatgataat atcgatattg ttgaaataaa tgatgatttg ttgcgtatta
    32581 ttagtgatct ttttgttata gaagctcatt tatccaataa atctaactct gctgactctc
    32641 tacctaaatt ttcatatact aacattcaat ttatcgaaga tagaatagat cattatgcga
    32701 gtacattaaa taaactaaat aaatttattt tgccaattgg tgattttaat gcgtcatttt
    32761 gtcatataat tagaaccgtt attagaagag ctgagagaag agtatgtgag ctaactgata
    32821 atgaagtcat accaaataaa gatttaataa tttcttacct taataggtta agtgattatt
    32881 tttttgtgct gagtagaaaa atattgcatt taaatggaaa agaagaaaga tattgggaat
    32941 attaattttt attttaaaat tttgtatgat ttttgcaaat aatattttta gaaatttata
    33001 aaaaccaaat agttatgatc aacttagaaa atattaaagc ttttaaggaa agaatagaat
    33061 cagtaggagt gcccatatta tctgtttatt gtgatacaga tccatctaaa gatgaaaata
    33121 aagggatggc gtggatgatt aggataaaaa atgccttaaa agagtataag gagttggaaa
    33181 agaaattaaa aaatcgaagg tcttttttag acgaatttat gttatcacta caaaaagtac
    33241 accctcaaac gcgaactttg gtgattttcg cgtgggtaaa cgaaaaaaat gtaatgcaaa
    33301 gtgaaacttt tgaattacaa atagatctac cagctatcga tttgacatta ggtagaatag
    33361 atgtacatta tggcaaacct tatcttttac ctatttatta tgcttttgat gagtacgaaa
    33421 gaattggtgt tttgcatatt tatggcacta aatggagatt ttatgaagtt tttttaaatg
    33481 aggttgagga aatcacagat acctttgcta ctattactac tgatgaatgg aaagaaatac
    33541 aggaacatct aaatgctatc gatcaaacat tatatggaga aagaaatgta caagcgagaa
    33601 taaaatttaa ggataaattt aaatctaaat acgaaaattg gtctcataaa ctatacaaca
    33661 aacttgctaa gatgatagaa aaatccatac aaacacttga cattaatcgt ttaatcttaa
    33721 tgggtacaga atggcaactt aacttttttg aaaattattt aagtcataat atcaaaaaca
    33781 taataattgg gcatttgccc aatcctacta atgcagaaaa tccttcaaaa aaggaaattt
    33841 tagaaagagt atcgactgta attgaaaaaa cagagtatga agaagaaaaa atgcttctag
    33901 atttagtgta tcaaggcaat ggcataagtg gtttatctga ggtactaaaa gccttgcaga
    33961 taggtagagt acaagtatta ttgttgcctt gggatttcaa tgctattgct tatatgtgta
    34021 gtaatgggtt aatattggct actaaagaag aagctgatca atattgtgga gatgctaatg
    34081 aaatttattt gagagattat atcgaagatc tgactaatga atattctaca agaatagaat
    34141 ttgtaaaagg tgaaataaaa gataaactct atacagattt aggtggtata gcaggtgtaa
    34201 ctagatggta aaaatcgata actatctttt aaatattttt aataatatac aacgtaaaaa
    34261 tttgcaaaaa gtatttattt tcataatttt gctataataa aaaaaattat gaaaacatta
    34321 aatatatata ttaattattt taagaagcat ttaaatgaat tagatctaaa tagagaacca
    34381 aaaacattgt atgatccgat aaaatatgga ttaggagtcg gtggtaaaag atttagacct
    34441 gcattgattt tattattatg tgatttttat ggtggaaatt tggatgatgc tataccagca
    34501 gctttaggga tagaattatt tcacaatttt acactttttc atgatgatat tatggacaag
    34561 gcacagctga gacgtggtaa agaaactgta gtagctaaat atgggctaaa tactgcacta
    34621 ttagctggag atactttatt tgcaatagcg tatagtatgg taaataaaac taatgaaaaa
    34681 catctcaaca gaatattaga agaatttacc aatactgcaa ctaaggtgtg tgaaggtcaa
    34741 cagttagata tggattatga aactcagata aatgttacta aaaatgatta tgaaaaaatg
    34801 atttatttaa aaacagctgt tttaatagca agttctatga aaataggtgc tatcataggt
    34861 aattgccccg ctaatgatga aaatttaatt tatgatatag gcattagtct aggtttggct
    34921 tttcagctac aagatgatta ttttgattgt ttcggtaatg aaaatacttt tggtaaaaaa
    34981 attggtggag atattgtaga tgctaaaaaa acttttttat atgtatctgc ttgtgaatca
    35041 ataaataatg aaaccgaaag aaaatatttt tatgatttat acaacaataa aaatatagat
    35101 aatgaagaaa aaaataaaaa aattatcact atttataaaa atttgaatat tgataaaaag
    35161 acaataaaac aaatagatta tcatataatg gaggctaaag atgttattca tcaattatca
    35221 atagataata ataaaaaaga cttaatctct caagtaatcg atgatatatc aaatagaaat
    35281 aaataaaaat attataccta aatataattt ttgttatcta taaagcatca caatgcccct
    35341 tttttcgaaa actttgccat ttattaaggt tatttttaat aaatatacgt ataattgttc
    35401 aggttctggt ttgccatcaa aggagttacc atcccaccct ttatctaaat cattagtttc
    35461 gaatactatt ctaccatttc tatcaaagat tgtgaaagaa taatcttttg cctttaaaaa
    35521 aataccacta ggtttaaata ttttattaac atctaaatga ctattcggag taaagcccgt
    35581 agggatatat attctaggag gttgaaacaa atccaaccat aatgaatgta tagtgtcata
    35641 atcgtcaaat ggattgtcta aatacactat ccattcgatt tgataagaaa aatgaccact
    35701 actctctata tcctctaagt ctactaaatc atctagatac gttctttgat caaatggcag
    35761 atttaattct aaataaggtt gattatcaat ttttttgtgc aatatttgtt gcaaattaat
    35821 tactgaactt gatgctttgc cctgccaaaa aagcgaattt gtaatatcaa aatttacagt
    35881 accgtttaag catatagtag acacagtatc actttgaaaa acttcattac cacagctatc
    35941 tatggtggct aatgtataaa aaatgggata ggatgtataa ttcgttaatg gatctacaaa
    36001 tatataatca ccagttaaat ttttttgtat tgttgcaatt tttttaaaac taattaaaga
    36061 atcagtagat ctatatagtg aaaatccaag tgtgctagtg tgtggagggc atttccaaga
    36121 aatattgatg cgattattta taaaatccgc ttttctaaat ttcacataag caggcaatga
    36181 aggttttttg aaattaaaac ataatacatt agatgattga gtattgccta aattatttat
    36241 agattgtata aatacacacc agtaacctac cgtatcatct attggtaaat taaaactgtc
    36301 tcgaatagta tttgaaattt ctataaaagg ttgtgtattt ttggataaaa atattttata
    36361 tttttcaata tttagaactc ctgtaggatg attccaagat aaataaaaaa tattattaca
    36421 aatatcaagc gaaccattta ggaatatagt ttcgatagca ttagacaatg gactagtgtt
    36481 accacaacta tctagtgctg ccactctata ttgctctgca ctttcatcag cattagagtc
    36541 agaatataca taataattgg tgccagatac agtatctatt ggtgtccaga aaccagattc
    36601 atatttgtaa ataatataag ctatcacatc agctgagtta gatggttgcc agcctaaggc
    36661 tgcagatcca ttacttaaaa ctgttacgga atctaaaaat ggtgattttg gtggcattag
    36721 attatgaaaa attccactag atatatttga tagtgaatag caaccactat tatctatttg
    36781 tttgatctga tatgatatag aagctgaaca agggtaaata gtatcaataa aatttgtact
    36841 aatggtattt ccgattgtag accatatacc agaaggatat tctcttagaa tttcataatt
    36901 tgaaaaacta gttaatggag gttgatgaag ctcattccaa gataatattg cagtaccatt
    36961 taatggatct gttaatgata aataaattgt tgaaagagta tctgaatttt cggtataatt
    37021 aataggagat ccgtatttag ctttaatgta ataatggact ttgtgcatgt tagcttgagc
    37081 cgatacatgt aaataattag ttgtatttat attgtttatt gtgctaatga gtgtatatgg
    37141 accattgata ttaacagaat aaaatatttc gtaagctata aaatctccat ttacatcatt
    37201 actaggtaac caattaatag acacatcacc attatttaat acttctaaac aacgaagttc
    37261 tggtatcgat acctgagtaa aaatgtaatg agggaaaatt actaaaaata aaaaaaagat
    37321 aatttgcttc attacacttt ttttttaaaa aaaacttata aaacacaact aaaatttatt
    37381 aaaataatta aaaacaagga aataaatatt taataaaatt ataaatattt aagtttttca
    37441 atatgtttta ttggttttat aataattttt ttaatttatc ttctaacatt ttttttgtaa
    37501 ctgctcctac ttgtttgtct accaattgtc catttttaaa aaataataga gtgggaatat
    37561 tacgtatacc atatttcatt gctatactcc cgctttcatc tacatttact tttcctataa
    37621 ataatttatc gttgtagatt ttatcaattt cttctaatac ttgtcccatc attttgcatg
    37681 gtccgcacca ttcagcccaa aaatctataa ttactggaac tttggcctcg cttattactt
    37741 tatcaaaatt gatttctgtt aaattttcta tcatgctttt ttatacaaaa ttatattttt
    37801 tattttattt gataaaaaga ttataaataa atttttattt aattaagttc atcaatttat
    37861 gttatatatt ttttaaattt ttaaatattc ctttttttaa tcattctttc ttttatatct
    37921 aatgtgttta ttaaaaaaca ttagtaatta aatgtgattt atcagaaatc attatacaac
    37981 gtaatgacta attttttaaa taacggtgga tttaagtata ttattatttt tttcttattt
    38041 tatatttatt ttttattagt gaatatagtc taatttatta ttataatgtt gtaattatgt
    38101 taatttttat gaagtttttt aaaaattaat tgtgtttaga agtttttacg atttggttta
    38161 tgtatttaat ctttgtttta ctttacgtat tttatactac catcaaataa tttttattta
    38221 attttacaaa aaaataatta tggataattt caatctatta gattatcaag ctctacccaa
    38281 agagcagaaa cgtagattcc tagataatct atatcaattt ttattagaaa acaactacac
    38341 agccgtagat tatcagaagc taaaaataca aagcacagcc cccatctgtc ctagatgcga
    38401 aagcgaaaat gtaataaaag ctggagtacg cgacggcaaa caagtgtaca aatgcaaaga
    38461 ttgcggccga caataccgcg aaacagctag aaccttcgta tatcgcatgc gaaaagctga
    38521 taaaatgctc gattacctaa aatgcatgtt agaaggcaaa agtctaagag cttgcgcatc
    38581 ggaagtaggc ataagtctga gaacgagttt caattggcga cacaagatac tagcagcgat
    38641 caaatcatta cagggtggta tcagcttctc aggcattgta gaagctgatg agctgctaat
    38701 gaaatattca gaaaaaggtc gcaaatacag aagccgaaaa gaatacgaaa aagcaaagaa
    38761 aaagaaacat cccaaagtag ctgtgctagt aatgacagac agggaaggca atttactttt
    38821 caaacacgta ggacgcaaaa aagtaaccaa tagtcagata agagaagagc tatcgcatag
    38881 agtgacagat agcaacttaa tatgtgtgaa gcaaaagaag gaattcaaaa aagctgtgaa
    38941 ggggaccaaa agcaaaaaag ttgttgtaaa caaaacgcag attagacgcg gtgtatacag
    39001 tgtacaagta gtcgataagc atgtaagtga tttcaaggtt tggttgcgac gatttagagg
    39061 agtggctact aaatacctgc agagctatct gatgtggttt gtaatcacac ataaatactt
    39121 gacaagtgag atagaatctg acattggtcg gcttttaaat ctcgcatcct cggacagatg
    39181 ggcgtgggat gagtattggc ggctggtaaa tacgaaatat agataaacta cttcaagtaa
    39241 aacaatgata taaaatatct caattaaaat ttttctttta tttagttgta aattacattt
    39301 atgttagggg taatattgat acttctctag agagttttag attgatatta aaccgaagtc
    39361 atgtaaaata catatataat aattttaacg ttttgatata agtttttagt cttttattgt
    39421 atcccttgct ctacctttta ttattttttt ttagcaattt aaaaatttta ttaattttgt
    39481 ataaaatttt atactttttt ttaatctcta catcttcaat gaaaaaaatc atttttgttt
    39541 gtttgacttt atttttattt gtcaatattc attctcaaac tttagatatg tgggaatttg
    39601 gttctggact ttctaaagct tttgatttag gtggtgaact aggaataaca tttggtacta
    39661 acactaatat tatggtttcc atcaaaggtg catatcattt taataattat ttttcattag
    39721 gattaaatcc tttttattgg tatataaagt ctcatttata tacacccgct atttctataa
    39781 atatgacagg tataagaggt tatttagata tttctgtttt tagggcgttg tatttacatt
    39841 gtgagtttga aaaaatgttt tatcgagcac catcactagt ttatccgtat tcagaaaaat
    39901 ggtttaattt agataatttt ttagtaggtg caggttttag gcaatttatt agttacaata
    39961 cttattcgta catagaggtt ttatggaatt tgaatgaaaa tataaattct gtctattaca
    40021 gtccagttat tagagccggt gttagttatg cttttcctaa taaaaaaaga tataataaat
    40081 cagatcttga gtaaatttgc attgtcacgt agccattttt tagcttctat gaaatttggg
    40141 taaataattt ccaatctatt ccaaaacgct tttgaatgat ttgggatttc taaatgtact
    40201 agctcatgta ttattacata atctaaaata tcctctggtg ccattatcaa tgtccaatta
    40261 atataaatgt tgcctttatg cgagcatgat ccccaacgtg tttttgcatt actaattttt
    40321 atttttgaaa attcaaatcc atatttatta gaataataaa atgctctatt tgttatttct
    40381 gataatgctt ttgccttata ccaatttata aatatttctc gagcacatga tattaaattt
    40441 ttagataaaa aaaaaagcat tatcaaatat caaagattga ttttgctcat ttaatattat
    40501 taaaggatag tagttgccta aatacaaaaa ttgttctcca ttaataaatt ttttaggtat
    40561 atatttttta tttctttctt ctatttcttt ttgttttttt tcgatccatt ttttatgttt
    40621 ttgtactatt ttatatattt cattttcatt taacttatag ggtgctctta ctacaagttt
    40681 attatcttca tttatttgta tggaaactgt actacgttta gaacgtatta ttttatctat
    40741 tttgatttca ttcatcagaa aataaatttt tacaaaaata ttattttaaa aataattaaa
    40801 tttttgcatt tttttaatat aaaattaggt tgttattcaa tatttattag ctttttgctt
    40861 atttggtttg ttttatataa ataaaaaagg gtaatcacca gactaccctt tttatcgatt
    40921 aaattatgaa aaaattattt tttatcctca ttattattta attgttcttt aatatcatca
    40981 aatataccta aatcagctag agtagtatga gtagaactat ccgattgtgc agttttagtt
    41041 tttttagatt tagatggttt aggagtttcc catgttctag tatgagaaac aataattttt
    41101 ttggtatcct tattaaattc aacaatcata aaatcaagtg tttcgcctga tttagctttg
    41161 gttttatctt ctttaattaa ttgacttgca ggagcgtatg cttccatacc ataaggtaat
    41221 tctattattg cacctttatc tttacgcgat tctattattt taccttgatg tatagaatca
    41281 ggagtaaaaa cagtttcata aacttcccaa ggattttctt cgatttgctt atgtcccaaa
    41341 cttaatcttc tattatcagt atctatttcc aaaactacta cttctagttc atcacctatt
    41401 ttgcaaaatt cagaaggatg ttttattttt ttagtccagg ataaatcact aatatggata
    41461 agaccatcta caccttcttc taattctaca aaaactccaa aggttgtaaa attgcgaacc
    41521 ttagctttat gtttggaacc tacaggatat ttttctttaa tatttaccca agggtctggt
    41581 aatagttgct ttattcccag agacatttta cgttcttccc tgtcgataga taatataata
    41641 gcttctaccg tatctcctgt ttttaaaaaa tcttgtgcag aacgtaaatg ttgagaccat
    41701 gacatctcag agacatgtat caaaccctca actccaggta taatttcaat aaaagcacca
    41761 tagtctgcta tagctactat tttaccacta actttatcgc cagcttttag attaggatct
    41821 aatagatccc atggatgtgg tgttaattgt tttaaaccaa gagcaatgcg ttttttatgc
    41881 tcatcgaaat ctaatattac aacgtttaat ttttgttcga gctgaactat ctcttcagga
    41941 tgatttattc taccccagct tagatctgtt atgtgtatta atccatctat gccacctaaa
    42001 tctataaata caccataaga agtaatattt ttcacttcac cttctaatac ttgtcctttt
    42061 tctagtttgg atattatttc agatttttgt gcctctatct cttcttctat gatcgctttg
    42121 tgagatacta ctacattttt aaattcttga tttattttaa ctattttaaa atccatcgta
    42181 gtgccaacaa atgcatcata atcttttatg ggttttatat ctatttggct accaggcaaa
    42241 aagcaatcaa taccgaatag gtctactatt aatcctcctt tagtacgtga ttttatgtag
    42301 ccttgaacta tttcattctt atcatatagc tcatttattt tgttccatgc tgagagtatg
    42361 cgagcttttt tatgagagag tataagctgt ccagtgccat cgtcttgact ttctacataa
    42421 acttcgattt tatctcctat ttttaaatct ggattataac gtacctcact taatggtatc
    42481 acaccatctg atttgtaatt tatatttaca aatacttctc tttcgccgaa gccaattact
    42541 gtaccttcta taacttcttt atcttgaagc acattcatag aggctgtata tttctcttct
    42601 aatttttttc tttcttcttc tttgtactct ctagtctctt ttaataatgc atcccagtcg
    42661 aaatcatcat tttctgtatt ttcttgtttt gcttcgacag catcagaagc ctcttcgatt
    42721 aattttacat cttcatcaat tgaaagtgcc tcactctcat tagagatctc ttctgtctta
    42781 tcaacctcat tttgattcat ttctggttga aaattttttt cattttccat tcatgttttc
    42841 tcctttctgt agaaaaactt ttaattaagt tttttgtttg caaaattata aatttatttt
    42901 taaatgaaaa ataataaata tggaatttta atttttttac aattttttaa atttaattaa
    42961 taatcatttt aatttcttat tttttttata taaaaaaatt gttacctttg caaaaaaatg
    43021 taattatgaa tttaacacat atagaacata tcggcatagc tgttaaaaat ttacaagaaa
    43081 gcataaaaaa atttgaaaac atgttgggta taaaatgtta tgctatcgaa gaggtaaaag
    43141 atcaaaaggt aaaaacagct ttttttcaaa tagggcaaaa taaaatagaa cttttagaag
    43201 gaacagaacc agatagtaca atttctaaat ttatcgaaaa gagaggcgaa ggagtacatc
    43261 atgtagcatt agctgtaaat aatatagaag gcgcccttca ggaattaaaa gaaaaaaaca
    43321 tacaattaat agatgaaaaa ccacgattgg gagcagaggg cttgcatata gcatttatcc
    43381 atcctaaaag tgcgggtggt gttttaatag aattatgcga aaataaaaac gataaataat
    43441 aaatattcat gtcaatagaa aaaaaaatag aagaattatt agaaaagcgt aagcaagcct
    43501 tactaggagg agggcaaaaa cgcattgaag ctcaacattc caaaggtaaa ttgacagctc
    43561 gagagcgaat aaacatttta ttagacgaag gctcttttga agagtttgat atgtttgtta
    43621 ctcacagatg cacagacttt ggtttagaaa aagaattata ttatggtgat ggcgttgtaa
    43681 ccggctatgg cacgatagat ggtagattag tatatgtatt ttctcaggat tttactgtct
    43741 ttggtggttc attgtcagag tcatatgctc aaaaaatttg taaaataatg gatctggcaa
    43801 tggaaaatgg agctccagta ataggtatca atgactctgg aggagcgcgt atacaagaag
    43861 gtgttcgtag cttagctgga tatgcagata tttttgaacg taatattttg gcttctggtg
    43921 tgataccgca aataagtgct gtttttggac cttgtgctgg tggtgcagtc tactctcccg
    43981 cattgacaga ttttatagta atgtctaaga aaaatagctt tatgtttgtt acaggaccta
    44041 aagtagtgaa aactgttact ggtgagatag taactgatga agaactcgga ggtccagatg
    44101 tacatgctaa taaatcaggt gtcgctcatt tcgtctctga gacagaagaa gaaggacttt
    44161 tacttattcg taaacttttt agttatttac catctaataa tctggaagat ccaccttttg
    44221 tagatactgg tgatcctatt gatagacttg atgattattt aaattatata attccagaaa
    44281 atcctaataa accttatgat atcaaagata taattactcg catagtagat aatggagaat
    44341 ttttagaggt acacgcacaa tatgctcaaa atattgtagt tggttttgca cgtttaggag
    44401 gtatgagtgt gggtatagta gctaatcaac caaattatct agctggtgtt ttagatatta
    44461 atgcttctcg caaaggtgct agatttgttc gtttttgtga tgcatttaat attcctataa
    44521 taacattagt agatgtacct ggttttatgc ctggcactac acaggagtat ggtggcatta
    44581 tagtgcatgg tgctaaatta ttatttgctt atggagaagc tacggtacct aaaataactg
    44641 ttaccttgag aaaatcttat ggtggagctc acgacgtgat ggggtgtaaa caattaagag
    44701 cagatattaa ttatgcttgg cctactgctg aaattgccgt tatgggtgct agtgctgcta
    44761 ttgaaatttt agaaagaaaa aatatattag ctatacaaga tgaggaagaa agaaaaaaat
    44821 atatagaaga aaaaattaat gaatatactg aaaaattctg ctctccatat gaagctgctc
    44881 gctatggata tatagatgat gtaatagaac ctagaaatac tagatttcgc attataagag
    44941 ctttatattc cctatctaca aaaaaacaaa atctcccacc taaaaaacat tctaattgtc
    45001 ctttataatt gataatatta tgaaaagagt tgttttgttt tttatgcttt tgttattagc
    45061 aatgactaat ttttcacaaa atatttctga tattagaata aatgaaattt caccaaatta
    45121 tgatggagat cttactaatc cactaggaaa taaaagtcca tggatagagt tatataatac
    45181 ttctctagta tcattaaaca ttggaactat gtatttaact gatgatagag aaaacttaaa
    45241 gaaatatcaa ttacctaaaa gtgatgtatt taatatatca cccaatggtt ttttagtatt
    45301 atatttagat actactgaac attgtcatcc tttggtagca cctttatcta ccaaaactgg
    45361 atatttggca ttagtattat ctaatggaag aactattata gactctttag tatttgatcc
    45421 ttccgcaatc gagcatgaaa catttctaaa aagaaaaatt gatggaactg gtgaatggac
    45481 tttaacaaaa attagtactc cattgctatc aaatgacatt aaagtctatg aaacccctga
    45541 tgagattgta aaaaaactcg atccttacgg tataggtatt tctattatct gttttagtat
    45601 aattattata atattgataa tactatattt ggctttttat tatattatgc aaattaaaaa
    45661 gccaatagct aaaattaaaa aaatagaaat caaaaaagaa ggtgaaacag taaaaaccga
    45721 aattatccaa gcagaagaag aaataagtag tgaagtcgta gcggccattg gtactgcttt
    45781 gcatctatat ctacttagta ttcatgatta tgaaagtact gttattacaa ttaaaaaaat
    45841 tcaaaaacct tattcccctt ggagttcaaa actctataat ttaacaccaa caccacaaag
    45901 aattcattta cccaaaaaat ttatttatga aaaaaataga tctaacaatt aatggaaata
    45961 aatatagtgt agaaatagga aatgtccaag ataatcgtgt cgaagtaaat gtaaatggca
    46021 ctatttatga agtggaacta tcaaaagaaa ttgaaatacc caaaacacca actctggttt
    46081 ctaccgttgt taataaatct gtaaaagaga ctataccaga accagtagag gtaatggcta
    46141 ctaaacaaat aagatcccct ttgcctggta cagtacttaa tatattagtt agagagggag
    46201 atacagtgca acgtggacaa aatttattga taatcgaagc tatgaaaatg gaaaatgaaa
    46261 taaaagctga cagcgctggt atagttaaaa aaatacatgt acataaaggt gataccatta
    46321 gtgaaggtca aattttagta gaaataggag gttgatttat ggatttttta ggttttgtta
    46381 ctcagaattt agagaaattt cttaactata ctgccttcgc taatatgagt tggggtaatg
    46441 taataatgat cttagtaggt ctttttttta ttgcattagc tattactaag cagtacgaac
    46501 catatctact aataccaata ggttttggta ttatattagg caatatacct tctgtgccag
    46561 gattgcaaat aggtatatac gaggagggta gtgtattaaa ttatctttat tttggtgtga
    46621 ttaaagggat ctatccacca ctaatatttt taggtatagg ggctatgaca gattttagtg
    46681 ctctactatc taatcccaaa ctctttatta ttggagctgc agctcaatta ggtatttatg
    46741 gtgctttgat tttggcattg ttatttggtt atactcctac tgaagctggt gctataggta
    46801 tcataggtgg ggcagatggt cctacagcta tatttttaag ctctagatta gcgcctcatt
    46861 tgataggtgc tattgcgatc tcagcatata gttatatggc actagtacct gtacttcaac
    46921 cacctattat gagagctatg actaccaaaa aagaacgaac gattcgtatg aaaccaccac
    46981 gtgctatttc taaaaaagaa aaggttttat ttcctatttt aggtatttta cttactacat
    47041 tgatagtacc atcaggttta ccattattag gaatgctttt ctttggcaat ttgctccgtg
    47101 agagtggtgt cactcgtaga ctagctgaaa cagcaagtgg tcccatgtta gatattgtta
    47161 ccattttaat tggtttaact gtaggtgcat ctactcaagc ttctactttt ctaactgtta
    47221 aatctttagg tatttttgct ttaggtgctt tatcctttat gattgctact tttggtggag
    47281 ttttaggtgt taaaataatg aatttattta ttaaaaaacc tgaaaataaa attaatcctt
    47341 taataggtaa tgcgggagta tccgctgtac cagatgctgc tcgtgtatca gaaatgatag
    47401 gactagagta tgatccatca aaccatttac tcatgcatgc tatgggacct aatgtagctg
    47461 gtgtgatagg tagtgctgta gctgctggtc ttttactcag ctttttagca taatttatta
    47521 gaaaaaaaag agcattcaaa taaatgctct ttttttatat taattttttt atttacattt
    47581 gctcataaat atataaagtt atgatctcaa taataattgg aagcatttct gatttcgata
    47641 ttataaaaaa ggcaattgat ttattagaag attttaatgt agaatatgat gtacaaatat
    47701 tatctgcaca taggacacca gacgaaacag ctaaatatgt taaagaagca actgaaaaag
    47761 gtactaaagt atttattgct gccgctggtt tgtctgccca tctacctgga gtagtagctg
    47821 ctaatactat agtacctgta ataggtatac caattaatac tagtttacaa gggatagatt
    47881 cattattatc aattgtgcaa atgccatcag gtatagctgt ggctacagtt ggcatcaatg
    47941 cagctcagaa tgcagcgttg ctagcaatcg aaattttagc tattaatgat gtcaaactta
    48001 aacaaaaact cattgattac aagaataaaa tgaaagaaaa aatcgctaag gaccaaaaag
    48061 aattagaaaa aaaattaagt aaataatctt ttatgagaaa aattttatta ttttcattag
    48121 tattgatttt gtcaattaat acattatcag gccaatttag ttatgagagt gcttctactc
    48181 ttggttatgg aaacagtgca tctgcagtac ctggtttctg gtcaggcttg atgaatcctg
    48241 cagggttagg atggttgaat aatcatcagc ttgggatcaa ctattacaat aaatatttac
    48301 taaaagaact tgggtatagg gatattgctt atgctcatgt gtttaatggt gatgcaggtg
    48361 ttatttcagc tcatatttct caatttggtg ctagttcttt attgtataat actttttcac
    48421 ttggttactc tagagtattt gctgatagag tatcagctgg tttaacaatc aaatattttt
    48481 tactcacttc taatcagcct gagtatagtg ggattggtac tattggtttc gatgctggtt
    48541 tacaagttaa aatcatcgat aacttgtatt ttggtacaat gattgctaat ttaaatcatt
    48601 ctaaacttgg aacttatgag tatgatagat taccaatagt tggttcatta ggatttatgt
    48661 atcaaatagc taaacagtct tcattaatgc tagatattct gaaggaaagc aataataacc
    48721 catcactcag atttggtact aatttctcat ttaatgatag attatacgtg agaggtggtt
    48781 ttcaaacaac accagctaga gttagttttg gtataggcta tagaatacaa aactttatag
    48841 tagacatatc aagctcatgg caccaaactc ttggtttttc gccttctatt tctatgagtt
    48901 atgcttttaa aaaagaacat caaaaaccta atataattta ttagaaatga aagctaagat
    48961 ttatatattt tttgtcttta tctgtttttt ttcatatcta tcaggacagg tagagataga
    49021 tgaacctgaa gagttagata ttatacagca aaatattgaa tatattgcag aggagagtgg
    49081 agtagaagaa gcggatttca gcgaattaac tgaaaaatta gaatactatt tgaagcatcc
    49141 aataaactta aataaaacta ctgtggacga attaagagat ttgggattat taactgacat
    49201 acagattatt aatttttttg atcatatcca gagaaatggt aaactacata gcttgtatga
    49261 gttgcaatct attgaaggtt gggatttagc aactataaat aaaatattac catttgtgaa
    49321 aataagtgac gtaggggtag ctcataaata taatttttca gaagtaatga aagaaggtaa
    49381 ttctcaatta tttatccgtt atatgcaaac tttagaaaaa caaaaaggtt acaaagctat
    49441 cgatgattct actttggctg ctaatcctaa tagcaggtac ttgggtagtc catacaaatt
    49501 atatacgagg tatcaattca aatattacaa tttgattagc tttggtttta ctgctgagaa
    49561 agatcccgga gaggaatttt ttaaaggcac acaaaaacaa gggtttgact tttattctgc
    49621 acattttttt attcgtaatg taggaccttt taaagctata gccttaggag attataattt
    49681 gcaattaggt caaggactta ctatgtggac aggtatgagc tttggcaaga ctgcagatgc
    49741 agtttttaca aagagaaatg ctaccggcat caagcaatac acttctgctg atgaaaatag
    49801 atttcttagg ggtgtggctc taaatgctaa aatttctaaa cgcctcgaaa tattgggttt
    49861 tgttagttac aaaaaaatag atgctagtgt agaaggtgat actatgagtt atgaagatta
    49921 ttatatatct agtattgtag aaactggctt acataataca gtgtcatctt ataacaaaaa
    49981 agatgctata aaacaattta taggtggagg taatattcaa tatacatcac taaataaaag
    50041 attacgtata ggggctacag cttttactac aattttagat aagcctttag gatctagaga
    50101 cgaactatat agaatgtatg aatttagtgg cactacactc aatgtagctt ctattgatta
    50161 tagttatatt tggaaaaatt tcaatatttt tggagaagaa gcatattcat ctaatggtgg
    50221 tatagctact attaatggaa taatggcggt aactgataaa aatttgtcat tttcattact
    50281 tcataggtat tttgatccca agtattttgt tttttacgca tcaccactgc aggagggcag
    50341 taaggcaagt aatgagcaag gtttgtattt tggactcaat gctaatttaa ctcataaatt
    50401 aaatctaact gcttattttg atttattttc ttttccatgg ctacgttata gagtaaacgc
    50461 tcctagtgaa ggtatggaag ctttggtaca gcttaattat aaatttgata aaaaaaattc
    50521 aatttatttt agatataaac atgaaactaa atatttaaat aaagatgatt tatatgtaaa
    50581 aaaattaata gaaaccaaaa aagataatta tcgcttaaat ggagtttttc agttgtcacc
    50641 atcatttaga tacaaattta gagtagatta tgtaaattat cgcaaaggtg atgattataa
    50701 tcatgatgga tttgcagttt ctcaaacatt gtcttataaa aagggtcgtt tcccattatc
    50761 ttttcattta acattcatat attttgatac ttatagctat gatgagcgaa tttatggtta
    50821 tgaaaatgat gtactttatg cttactcatt cccatcttat tattataaag gtgctagata
    50881 tgtgattaat atcaaatata ccattaataa atatttggat ttttgggctt atttagctcg
    50941 tacaacatat tttgatcgca atgtaattgg tagtggatta aatgaaatac aaggaaatca
    51001 taaaacagat ttacatttgc aattgagagt taagttttaa acaaaaatta ttataaaaac
    51061 attattgctt caaatttgct agcatcaaag caaatcaatt tctagttata taaatattta
    51121 aaatcaagta gtaaattaat aatttattaa cgatattgat gcacgatata gattagaaaa
    51181 aaagtgttta gatggttgaa attaataaat gtttaagttt ttcaagttta tatgttaaat
    51241 aatttttata tttttacaaa aattattatt catgaaaaaa atagtggaaa acaccagtaa
    51301 ataccttgct atgagcaagg aagagaataa agaattctta gacaaccttt attctctaat
    51361 gaaaaaatta gaagccgatg tagtcgatta tcaaggtctg aaaatactca gcgccccgcc
    51421 actgtgccca gactgtcgaa gcaatcatat tattaaaaat ggtttacaaa atgggatgca
    51481 aaactatcgc tgcaaagact gtggcagaca gtttagagtt accacaggta cttttgttta
    51541 caggttgcaa aagaaagaga aaatgctcga ttacatcaga tgtatggtag caggcaaaag
    51601 tctcagagct tgtgctaaag aagtaggcat cagcctacca acaagcttcg cttggcgaca
    51661 caaaatattg gcagctttac aaagtttcga agataatatc aattttttcg gagtagtaga
    51721 gatggatgaa ctattgatgg attattccga aaaaggcaga atatacaaaa atgcagaaga
    51781 gctgaagcga gctaggaagc aaaaaccaaa gaaagtagcc gtattggctg ctacagacag
    51841 agctggcaac ttattattca aaaaatttga tggcaaaaag ctaagttcag aacagataga
    51901 gcaatttctg aaggctaaga tgccaaagga tggtgtcatg tgtagcagta ataaaaaagc
    51961 attcaaaaag gttagaagaa atgtaatcaa gcacaaggaa atagtaacta gcaataggca
    52021 aaagcgaggt atatatagcc taagtaccgt caagaaaaag gctagtgaat tcactaattg
    52081 gatatatttc aaatttaggg gcgtagctac taaatacctg caaaactaca ttatgtggta
    52141 tgtagtgatg ggtaaatacc tcacaggcaa gactgtaagc aatactgggc gaatgctcaa
    52201 tcttgcatcc tcggacaggt gggcatggta tcggtataga gagttagtaa atataacata
    52261 tctaaactta aacatttatt aattatccac tccccttaac cacacttcta taccaaaaac
    52321 gcatctatac taatctataa taagaaataa gataattaat gttttaaatt aacaatttaa
    52381 tgaatttagt gaaatttgaa tttatgtttt taaagtttat aatataaaag aatttttttt
    52441 agaatccaga tgcaccaaat taataattat tgagaatttt tttatttttt tataatttat
    52501 ttttatcatt tgtttaacgt aaaagtattt catctgttaa aaatactaga taaatgcata
    52561 tgataaaatt ctttattatt gtatgagttt agaataaaca aaaaaataaa gtttagtaaa
    52621 tttgttttta ctttttattt ttgcaatata aatttatatt atgttaagtt cagaagagag
    52681 aaaagaaata aaaaatttaa tacaaagtga gataacgcct gctattgggt gtacagaacc
    52741 tatagctgtg gctctttgtg tagctaaagc aaaagaaata ataggaagca tcccagaaaa
    52801 aatagatgtt ttattgagta aaaatattat taaaaatgct atgggtgttg gaatacctgg
    52861 aacaggtatg gtaggattac ctattgctat tgcattagga gctttaattg gcaaatcaga
    52921 aaataaatta caattattaa aagatgtaca gcctcgggat gtagaaaaag gcaaaaaata
    52981 tatttctgaa aatagaataa atatttattt aaaagaaaat tgtcctaata acttatacat
    53041 agaaacacat tgttatgttg atagtgaaaa atctgttgtt attatagaag gagctcatga
    53101 taattttact tacatagaaa aaaacaataa aatattatta aatcaaagct atgaatctaa
    53161 tgacaaaaat gataatgttg tccaattgac atttaataaa atatatgaat atgctataga
    53221 gacacccata gaagaattag aatttatttt agaagccgca gatatgaata aattagcatc
    53281 catacagtct tttattggaa attatgggca tcaaatttca aaaattttaa caagtgaaaa
    53341 ggcaaagtca attttaggag ataatatttt gaaccgaata atagccgcta cagctggtgc
    53401 ttgtgattta cgtatggatg gagctatggt tcctgtgatg agtactgctg gtagtggtaa
    53461 tcaaggtata gcagctacta tacctgtttt gatttttgct gaagaaacaa attgtgataa
    53521 aaaaaagcta atacgtgctt taatattaag taatttgaca gtaatatata ttaagcaaaa
    53581 aataggtaaa ctttctccac tttgtggttg tgttgtggct tctactggtt ctagttgtgc
    53641 tattacttat ttaatgaatg gtaataaatt acaaatatct catgctgcta aaaatatgat
    53701 tgccagtatc actggcctaa tttgtgatgg agctaaacca agttgctctc taaaaatagc
    53761 taccagtata tatactgctt tattatcttc attaatggca atagataaca aagtggtctt
    53821 atctaacgaa ggtatcattg atgatgacat agataaaact attaataatt tagctgctat
    53881 tggcaatata ggaatgaaaa atactgataa tattatttta gatattatga ctaaaaaaat
    53941 atctaagagt cctatgaaat attaatttaa aacaatacct ttgattacta ataaaataat
    54001 tttgaaatat atgtgtgaga atgatttaat atatatgcgt cgctgtttaa atcttgcaaa
    54061 agaaggactt ggttatgtta ggtcaaatcc tttagtaggt tgcgttatcg taaaggataa
    54121 tcatatcatt ggagaaggat ttcaccatgc atttggtaaa ccacacgcag aaattgaagc
    54181 aataaataat gtaaaagata aatcacaaat aaaaaatagc actatatata taaatttaga
    54241 accttgctca cattatggta aaactccacc ttgttcgcaa tctatcatag atgtacaacc
    54301 tcttagatta gtaatagcag atatagaccc taatccatta gtaaataata aaggcaaaga
    54361 aattttagaa aaagctggta tcaaagttga tgttggctta tgtaaaaatg aacatagaca
    54421 tttgaatagg cgtttttata ctttttatca gaaaaatcgc ccctatatta tacttaaatg
    54481 ggcacaaaca ctagatggct tcattgctca aaaaaatcaa caatgtatta aatggattag
    54541 taatgacgct agcttgcaat tagtacataa gtggcgtaca gaggaaatgg caatacttgt
    54601 tggtgctgaa acaattagat gtgacaatcc tcaattgact tctagacatt ggtatggaca
    54661 caatcctatt agattagtag ttactaattc aagtaatttg ccaattaatt ctaatatttt
    54721 taatgatcaa gctgaaacta caatttttta taaaaattta attaaacaat acccttcaaa
    54781 tatagatcta gtaaaaatag aaaatgattt tttaggttca ctatttgctt actgtttaga
    54841 aaaaaacata atatctattt tagttgaagg tggtgcttct actttagata attttattaa
    54901 acgtaatcta tgggatgagg caagagtgat aataggtagc aatatttttg gtaatggtaa
    54961 aaaagcccca attattgatg aatataaatc atttgacatt tttgatcttg aggataataa
    55021 aataattcat tattataatg atacttatta aaaataaatc ctactcacaa ataagcgcaa
    55081 cagacaaggt atggggtaaa aaaaggggaa gatactatag agtttagaaa gtagtacatt
    55141 gttagttaga ttatttgatt tttatttaga aaaaatgtaa aaaaatattt atattgtttc
    55201 ttttaaaaaa attatcctat ttttgcaaat ataaaatgaa attatatgat acaaattaat
    55261 gtcaaaaata tttcacatca tccattgcca gattatgcta cactgtttag ttctggtatg
    55321 gatatatatg cttatataga caatgaattt gtaatgaagc ctatggagcg tgctttaata
    55381 tcgacaggat tatatataga attaccacct ggttatgaag ctcagatacg tccgcgctca
    55441 ggtttagcaa ttaaaaatgg tataacagta ttaaatactc ctggcacaat agatgcagat
    55501 tatagaggcg aagtaaaagt aatactaatt aatctaagtg atcaaaattt tacaattcat
    55561 gatggtgata ggatagcaca aatggttatt caaaaaatcg aaagagcatt atggagagaa
    55621 gtagatgaaa taagtgatag tgaaagaggt ggagggggct ttgggcacac tggtaaataa
    55681 ttaatcttaa aatatttgaa tctatgaaaa ttataattcc aatggctggt ataggtaaaa
    55741 gaatgagacc acatactctc actataccta aacctttgat tccattagca ggtaaaccaa
    55801 tagtagagcg aataataaac attttaattg aatcattaga tagagaaatt gatgaaattg
    55861 cctttgttat tggcgacttc tccgaagaga tacagcaacg ggtagcagat ttaggtaaca
    55921 aatttaatat taaaacttct atttatcgtc agagtagccc actggggaca gctcatgcag
    55981 ttttagcagc cgagccatca cttaaaggag aagtcatagt tgcctttgct gatactttat
    56041 ttttctctca atttaattta aaaaatatgg atgcagatgg cattatatgg acttgtgaag
    56101 ttcctaatcc taaggactat ggagtagtaa aacttgatga tgatggttgt gtaactgaat
    56161 tttttgagaa acctactaat tttattagca ataaagctat tattgggtta tattatttta
    56221 gaaatgctga aatattaaaa aaatctttat ttaaattaat agaaaatgat caaaaagtta
    56281 atggtgaata ccaattaaca gatgcactac aatatatgat agaaaatgga tacaagataa
    56341 aagctcaaaa cgtagatgaa tggttggatt gtggtaacaa aaatgcaact ttaatagctc
    56401 ataaaagaat tttggaacgt tatcctgatg aaagtaaagt aaatgaaagc gttaaatgca
    56461 aaaacgctat catagtacca ccttgttaca tagaagaaga tgtgatctta gaaaactcta
    56521 tagtaggacc ttttgttagt attggcaaag attgtataat ctcacattct atcattacta
    56581 attctattat tcaaaataat gttaaattat ctaatgttaa tattgataat tctatgatag
    56641 gtaaatatgt agattataat gatagttatg atgagttaaa cattggagat tattctgaaa
    56701 tgaacaaatg aaattattag catataaata ataaataaaa gtttatttat atgattaaat
    56761 tatttgatac tagagtatat tattcgctag ctctaccaat agcttcttta tgaaataata
    56821 atgaacgtat aagttcggct atacaaataa ataaaattga aaagaatatt tctttgataa
    56881 taaataataa tcttagatac atccaagtag tgaagtactc aataatagca gtagtaaatg
    56941 aaaatattat tgtaaaaatc gtaatataga gtagaaaacc tggccagcct agaagatctg
    57001 aattagcata aagtagctta tctgcttttc gatacttatt aaattgtggt aatctaaaat
    57061 agtaatattg taaaaacatt aataatgtag atgcaaaagc atataatcca taagtaaatg
    57121 taaataaatc tctaagcata cctatagcaa aaccgattaa tagtaatagt gttttattgt
    57181 aattaaatgg tataaggcat aaaaaaatat atataaacaa attaatctgt ataccccacg
    57241 ataatggtaa atgatctaat attagtattt gaataaaaaa aatcagaaaa gcaaataatg
    57301 tttttttgaa ggtagacatt tatttattta ttttgatata atatatttat ttcatcacta
    57361 tctaagcaaa ctacagtata agtgtaatag acacttatta aatcttctaa atatctaaat
    57421 ttgtagcgtt tagtttggta aatattatca gaggtttttg ctgttataat gccgataggt
    57481 ataccaggcg gaaacaaagt agaataatga cttgtataaa tagtatcgtt tatattaatt
    57541 tttactgtaa aaggtacatt tattatttct ccattattgg gcgtatgcat atcccattga
    57601 gcgatgccga cataattatt tttaatagca gcagaaattt taacatctgg atgcatatat
    57661 gttttgacta aagaatatcg ttcggatacc caagttatta cacctaatat gcctttagga
    57721 gtaaaaactc cgctattttc ttttattcca tcccttttgc ctttgtctat aacagcataa
    57781 tttttagatt catgcaaagt cattgatatc aatcgtgcag gtaaaaattg atataattgt
    57841 tttgatgtat ctgaaattaa ataatcaact tgattatcag atatacatcg aatatttaca
    57901 tattgattat ttttattata caaattaata ttttcataaa ataatgcttc attttctttt
    57961 tttagatgca tataatgaga taaatctttt gaaaagctat taataggatt ggtaataaat
    58021 gaagatattt gaaaaaaata tcctttgtga atttcagaat tataaatata tagccataaa
    58081 gaaaaaatag ataatattaa aaatacaata aaaaaattat attttttaaa taatttgagc
    58141 cagttccaca tgtactaaat tagaaaggga aatttattaa aatttttaag agctacacta
    58201 gcaccgcgag ctactgctaa taaaggatca tcgccaacct ttacttttag tttgatgcgg
    58261 gtagataatc ttttatctaa accacgtaaa agggcaccac cacctgttag gtaaatgccg
    58321 ttttgcaaaa tgtcggcaga taattctgga ggtgtatatt ctaaagcatt aattactgta
    58381 tcttcgattt tagaaatagt tttatcgata gcatgagcta tctcttcgtg atttacatgc
    58441 actgagcgag gaatacctgt gataatatct ctgccattta cttccatagg ctctggtgga
    58501 ttttcaatat ttggaatagc ggcacctact tcgattttta tacgttctgc tgttctttca
    58561 ccgatactta gattgtgagc acgtctcata taatcttgga tatcttcatt taattcatta
    58621 ccaccaattc taatactttt attacttaca atgcctccta aagctattac tgccacttca
    58681 gtagtgccgc ctcctatgtc tacaatcata ttgccataat tatctaatac atctattcct
    58741 atacctagtg cagcagccat aggttcatga attagtttta cttctttagc gccagcttgt
    58801 tcagcagaat cgtgtacagc acgcatctct acctctgtga taccacttgg tatacctatt
    58861 accattcgat agctatagtt tattattgta tttttccaag gtatcatttt tataaattct
    58921 cttatcataa tctcagcagc atagaaatca gctataacgc catctctcaa agggattata
    58981 gtctttatat gttcatgtgt tttgccatgc atcatcatag cttttttgcc aactgcaatc
    59041 actgtatttg taatattatc tatcgctact atagaaggct catttactac tactttatca
    59101 ttatataata ttattgtgtt agctgtacct aagtctaccg ctatttcttt tgttaaaaaa
    59161 ttaaataaag ccatcttatt cctttatttt ttaatgttta aaattcctta tttttgtgaa
    59221 tgacaaaatt atatcatttt cttgacaaaa agatataata tttttgtcat gtatagatcc
    59281 acctggttct actacagcga ctataccatt ttgataagct aaatctatac tatctgtgaa
    59341 gggaaaaaaa ccatcactag ccaatatagc tccagtgaga tcatgaccaa atgtttttgc
    59401 tttttctatt gctatcctag tagaatctac tctacttggt tgaccacatc ccatacctag
    59461 taactgatta tctttgacta tagtaatagc gtttgatttt aagtacttga caatagatac
    59521 tcctaatttc atgtcatcta ttaatttatt attcaatttt tcatttttca ctatccattc
    59581 ccacttagat gaatctggga tgtaatcatc tctttgctgt actaaactac cacctaagac
    59641 gcttcgccat tgccaattat taggcttagt ttcagagata gaaagtaaaa ttctattttt
    59701 tttgattgat aatttctcta tttgaccttt tgtaaaatga ggtactataa gcacttcaaa
    59761 aaataaacta tttaattttt ctattgtggc atcatctatg ggagtattga aagcaaaaat
    59821 accaccataa gcagaaacag gatccccagc gagagctcta tcgaaagcat ctattggact
    59881 tttgcctatg gctacaccgc aggggttagt atgtttcact actacacaag atggttgatt
    59941 tagctcattt actagtaacc atgcactatc tatatctaaa aaattattat aggacaattc
    60001 tttgccgtga tgtttttcgt atctcatgct ctcatcatgt agaaaaaaag cctgttgatg
    60061 tggattctcg ccatatctaa gaggagttac caaattttta ctaatgattt tgctttcttg
    60121 tgctaaataa ggttgtgaaa accattgaaa tattgtagca tcatacatcg ttacgatagc
    60181 aaaagaattc ttagctaaat gacgcctgtc atgctcttca ctatatccat tttttataca
    60241 ctcaaagaaa tgaggatagt ctctgctatc agctgctaca agcacatctt tgaaattctt
    60301 tgccgcagca cgtatgagtg tgataccacc aatatctatc ttttctatta tttcagattc
    60361 atctatactt gtttttatgg tttctgcaaa aggataaaga tccactaaaa ctgcatctat
    60421 ttcttctaaa ttatactttt tggcttcttt tttatcattt tcattatctc ttctgagtaa
    60481 tatgcctcct ataataactg gatgtagggt tttaactcgt ccatctaaga gggctggata
    60541 attagtcaaa ttgtcaattt ctattacatt tattccattt tcattgagaa atttactagt
    60601 accaccagta cttattattt tacctttgta atttttcaag atatttagat agggtaggag
    60661 aacgtcctta ttataaacag aaattaatac tgaatttatt gttttaatag acattttttt
    60721 gcaaagataa aaaaagaaaa ttgtatttta aattttaatt attttttaat taaaatattt
    60781 agtatttaaa atcttcagag aataaagccg aactatttag aatttattat tcttatgtta
    60841 tttattaatt atttttaaat aattccagta aatcccataa atgctaaaga caagatacca
    60901 gcaagtatca aggctatagg aacaccttga aatgcttttg gaatattcga aaattctaat
    60961 ctctccctta gaccagccat tattattaat gctaagccaa aaccaatacc aatagatata
    61021 gcgtagacta tcgattctcc aagattataa ttttttgtaa ttactaataa agctacacct
    61081 aatacagcac aattagtagt aatcagaggc aaatagatgc cgagagcttg gtataatggt
    61141 tgagaaattt ttttaataat aatttctacc atttgcacta aagatgctat cactaaaata
    61201 aaagcaatag tttgcaaata ttctaaatgc aaaggagcta atatgccata ataaactaag
    61261 taagtaacta aggtagctaa tgtaaggaca aatactactg ctgcccccat accgacagct
    61321 gtactcattt tttgtgatac acctaaaaat gggcaaatac ccaaaaattg tgttaataca
    61381 acattagata caaaaattgc tgatattata ataattaaat attccattac taattatttt
    61441 ttggtttatt agacatttta tttttatata aattattcaa tgccattaat attcctaatg
    61501 ttaaaaaagc gccaggtgct aaaataaaaa ctaaaatacc atcagatgta ggagctataa
    61561 atttgtatcc aaatatactg cctgcaccga ggatttctct tatcatacct aaaactgtta
    61621 gaatcaatgt aaatcccact cccatcccca agccatctat taatgatgat aagacattgt
    61681 ttttagaagc aaaggcttcg gctctaccca atacgataca attaacaact attaatggta
    61741 taaagatgcc taaagcttta tataaatcag gtacaaaagc atgcatcacc atatctataa
    61801 tagttacaaa agtagctatt ataactataa aaacaggaat gcgaatgatt ttaggaacaa
    61861 ttttacgaat agatgaaatt attacattac ttaatgttaa aacaaaagta aaagctaagc
    61921 ccattgctaa tccatttatt gcagaagaag taacggctaa ggtaggacac atacccaaaa
    61981 ctaatactaa tactggattt tcttttaata ttccttttga aaaattttgc cactgattca
    62041 taattttatt ctcctccttt ccacgctttc aatgatctat atgctctatc tacagcatca
    62101 caaaaagctc gagatgagat agtagaggcg gtgatagcat ctacatcccc attatctttt
    62161 tttactgata acttaaattt ttctggattt ttacctctga attgatcttt aaaatcagat
    62221 tgagtaattt tttcgcctaa accaggagtt tcagcatgac tcaaaacctg tgtattgata
    62281 atagtaaggg tggtatcgaa acccaccatt aactccattt taccattaaa cccttgcatc
    62341 gtatatgtac tgactgcata gcctactatt tgaccattgt atgatgcttc aaaaacttta
    62401 agtgaatctt cgccatcgat gtttgggata taaaaagttt ttaaatcaga aaaagacggt
    62461 aatacttctc ttagtgcatc tttttctttt tgttgttgag ctttttgtat ggggcctgta
    62521 gtaattaaat taatcaaagc caacattata gcagctacag ctgcaatgat aaaaagtgta
    62581 agtgctaaat ttgataaact tgattctctt ttactcatgc ttttacctcc ccgaaatgtt
    62641 tatatgatat tctatctata agtggtacaa aggcattcat aatcaaaata gaaaatgata
    62701 cgccctctgg gtaggcacca aagagcctta tcaatgccgt taatactcca catcctatgc
    62761 caaaaattat ttgtcctcta tacgatgtag gtgatgtgac catatctgta gccataaaaa
    62821 aagctcccaa aatcaatcca cctgttaata aatgaaataa tggatctgcg tatttagtag
    62881 ggtctatcaa ccaaaatatt ccagtgaata ttatcacggt caaaatatag gatagtggaa
    62941 tatgcaaggt aataactttg cgatacaata aataaatagc accaatcaat aataatatag
    63001 ccgaaacttc tcccatactt cctcccatat tgccaagcat catatctaca tatgatggca
    63061 attcgccagt ttgactagct gctgatatag aaccactttc ttttaatatt cctaatggtg
    63121 taggtccagt aactacatct gacaattgac ctgctgatgc aataggctta ggccatgaag
    63181 tcatatatac aggaaatgaa attaaaagaa atactcgagc tacaagagca ggattgaata
    63241 aattttgtcc taaaccacca aagaccattt ttcctatacc aatagctatt agcgaaccaa
    63301 tgattactac ccaagtaggt agattactag gcacattaaa agctaaaagc atacctgtaa
    63361 ctatagcaga gagatcattg atagttggct tttgctttaa taaaaatttt tgaattaaat
    63421 attcgaaaat taaacaagta actaccgata caaaaaatac tctgacagca cctaatccaa
    63481 aaaaataaaa tgcgccaagt gtagcgggta ttagagctat cactacatct agcataatac
    63541 gctgtacatt atctttagca tatatatgag gcgagccaga gattataaat ttcttatcca
    63601 tatttgtatt aattttttcg agatcttatt attgcactca ctttgttttt gcctaatcta
    63661 atataatcta ataaaggtct tttagcagga catatatatt ggcaagagcc gcattccatg
    63721 caatctatta tccaatgttt ctctgcgtct tcccatcttt cttttatgac tagtttttct
    63781 aaataaaaag gttctaaacc catagggcaa gcatggacac atctaccaca tctaatgcaa
    63841 ggataaggat ctaatctttt tgataaattg tatggaaaca gtaaaatacc tcccattgct
    63901 ttagtcaatg gtatgtctgt atttgatagt gttttgccca tcattggtcc accactaatc
    63961 aatttagctg tatcttcggg taaaccacct gctaattcta tcaaatgctt caaaggtata
    64021 cctattcggg ataaaaaatt agatggtttt gatacattag gtccggtaac agtaaccaca
    64081 cgctctatca aaggcatatt tttttgtaca gcatcataga tagcttttgc agtgcctaca
    64141 ttttgaacta cacaaccaac ctctattggt aatttaccgg atggaacttc tctacctgtt
    64201 atagctttta ttaattgctt ctcagcacct tgtggatatt tctttttcaa agtaactact
    64261 tctatgccat tataatcttt tgctaattta gttaataact ctatagcatc tagtttgtta
    64321 ttttcaatac ctatgaaagc tctatgtact cctaaagctt tcataataat agaagtacct
    64381 actaagattt cttcaggttt ttcgagcatg agtcgatgat cacaagtaag atatggttca
    64441 cattctgcac catttattaa tagatattct gctactttgc cttgcggaat ggtgagtttg
    64501 acatgagtag gaaaggccgc tccacccaag cctacaatac cagcattttt gattttttcg
    64561 atgatttctt gtggagttgc ttttatctcc tttatcaggt tattagtaat atctatacct
    64621 tcttcccagg tatcttcacc ttctacatta ataataatag cttggtgttt ataaccagta
    64681 gcatctaata tcggatctat tttatctatc gtaccagtta caggagaatg aacattagaa
    64741 gagataaaag cttcaccttt agctatcaat tgtccagttt tcactttatc accttttttt
    64801 actataggta ctgcaggggc tccggcactc tgatttagca ttatataaac tttatctggt
    64861 agagtagcta caaaaatagg agatttagca gttagcttgc aatcatctgg gtgtacgcct
    64921 cctttgggaa atgtttttaa catgatttaa ttatttacta tattttttga tgaatcattt
    64981 gcaatattgg cgttttcctg ttttttaggt aaaaaattaa cttctaaaat gcaattagta
    65041 ggacagacag caacacattt acgacacaga gtacattttt tataatctat ataagctaag
    65101 aaattttcaa tttttatagc atcaaattta cattctttag cacaagcatt gcatcctata
    65161 caagctacca agcagtttgc tttagcataa ataccttttt ctttattcat gcaagctaca
    65221 taaattctac gatttttagg tcctttattt ctaagctcga taatatttct agggcaagct
    65281 tttacacaat taccacaagc agtacaatta tcttctatta caactggtaa acctgtaata
    65341 ggatccatgt acaaagcatc aaatttacat gcttcaacgc aatctccaag tccgagacat
    65401 ccataagcac agccaccttt gcctgcgaag agaaaattag caaaaccaca gttttttatt
    65461 ccgttataat cgtgtttagg aggacaattt tcaaaagaac cttgacaacg aatgacagct
    65521 acctgaggct ttttctcttc agcagttaaa ttcattaatt ctgctatttc gctcattact
    65581 ttattaccac caggtgcaca aaaaagacca tcgagcgaat tgtttttaac tatagcttct
    65641 gcaaaatttc tacaccccgc ataaccacaa ccgccacaat tagcaccagg caatttctct
    65701 gctattaaat ctattcgggg atcctcttct acgtgaaatt gtttatcaac aaaataaata
    65761 ataactgcaa aaatagcagc cataagtgat aatacaacaa atgcaataat gacaggttcc
    65821 acttattata attttattgc aaaataaaga attttttttt gaaataaaaa aattattaaa
    65881 tgattaataa aaataattat gaagtaaaaa aatattattt aattttaaaa tattagaaag
    65941 taatcataaa aatataattg ttcggtaata ttttatgatt tttataacaa tttttttata
    66001 attattatag atatattttt aattttgcaa aatcttaatt aataatctga taagcaatag
    66061 aagaatgtat gatagtgaat gcttttttga aaggaatacc agtaggttta atattggcat
    66121 ttagttttgg tccagctttt ttcttggtaa tacagacagc tttagaaaaa ggatttaaaa
    66181 aagctatagt tacatcgata ggagtttcta gtagcgatgc agtatttatt tgcttatcat
    66241 tattaggtat aacggccatc gaaggattac tgaaagatgt ttttggggta atagcaagta
    66301 ttgtgcttat aatatatggt gtagttatga tcttttctaa accagaaata cttaagaaaa
    66361 gaaatcctaa agtaaaagat attcgtagta aaaatgaata tattaagtta tatttcaagg
    66421 gatttttatt gaatatagct aatccttttg tattgatttt ttgggttagt atagctggta
    66481 tagtttcgca aactgctcct aaggatcaat ttttattata taatattttc ttttttctag
    66541 gtatcttatt tactgttttt tctatggatg ttatcaaaag tctgacggct cataaattaa
    66601 aaaattattt gaggccaaga tacataatat atgctaatag aataattggg tgtattatag
    66661 taatattggg tgttttactt atttttaaaa cttttatgat aaactaatct actacgtata
    66721 ttatgatata ttgtgaagat aaatgatgaa tttactttaa ctcagctggc ataaaaaatt
    66781 actgattaaa tgcataatgt attatgttag ctcccatttg taatgctttt agtctgattt
    66841 ctggaggatc attatgcact tcttcacttt cccatccatc tcccagatcg cattcatagg
    66901 tatatagcaa aactaacctg ccatttagaa ttataccaaa tgcttggggc ggtttactat
    66961 cgtgctcatg aatttttggc aagccattag agaaattata tacttggcta aagatagggt
    67021 gtgatggggg taactcctgc agtggtagat caggaaagat tttttgtatc tgcggacgta
    67081 tgaaaggatc cataccataa ttatcatcga tatgtaaaaa accacctgct tctaagtatt
    67141 ttcttatatt cgctgcttca ttatccgaaa aaatcacatt accatggcca gtcatatgta
    67201 taaaaggata attaaaaatt tcttgtgagc ctggttctat ctccggtata ttaggatcta
    67261 tattagtatt aatattttga ttacaaaaaa cagcaagatt tggcaaagat gttgggttag
    67321 cataccaatc acctccgcca gaatatttta ataaagctat ttttatgcta taaacatttc
    67381 tttgggcaaa agataaattt gatattaata gtaataatat tattagaaat tttttcataa
    67441 attatttttt attgcaaata tagttaaact tacaatagcg gcagtttcac ttctgagtct
    67501 ttgtttacct aatgacactg gtatgatacc gttataaata gccttattga cttcatctat
    67561 tgtaaaatcg ccctctggac caataaaaat acctatgtct tctttaggtt gtaagaaatc
    67621 aaaaaattgt gttttctcta attctgtacc acaatagcag attgtacgtt tcatttttat
    67681 attatttttg atagcttctt cgaaaaaaat cggctcacaa atatgaggga aataaaattt
    67741 cagagattgt aatgatgagt attttattat attttgcaat ctcgttagtt tgatagttgt
    67801 tttgatagtt ctctcagtga taataggtat taattcactt actcctaatt ctaccgtttt
    67861 ttctaccagc cagtctaatc tctctgtctg ttttaatata ggtgtgtata gtattgcttt
    67921 attatcacgg ggaggcatag ttttcacctc tagtacatcg actattattt tttgtttcat
    67981 acattctact attttgcccg tagctagaag tcccttacca tttgtaaaaa atattttatc
    68041 tctagttttt atatgatgag ctctcaaatg cttatacgtt tcaccttcta ttactactct
    68101 atctctaata ttttctaaat aaaataaatc catatttttt gataaaattc tatatcaatt
    68161 tattaattga tgcaaaatta taaatcttta attaaaaaaa aacgttgatt cgcttttaac
    68221 ggtgtaaaac taaatgctaa tgactcaaac accatacgtt tatgtttttt gataattgtg
    68281 attttttaca ttaatagatt gtctgttgca tttatttgaa ttattttttt taaaataaat
    68341 ttaaaaaatc acacgaaaaa atccagaatt cacttatcaa aataacaaat aattgataaa
    68401 ttatttttct ccaggttaga tcttcggcta ttaaaatttt ttgaatattt agtgtcaaaa
    68461 gccatacata tatcaaaagt acgattttga agttatctaa aggtaatgta aaagctatta
    68521 ttatgcaaat taatatctga actattgata cccagatctg tcttctcttg tatactaatt
    68581 gtctctgcat ccacggataa atcattatgc aagtaacaag tgaaaaaatt gtaaatgcta
    68641 atagtggtaa agacaaacta gctgatgaca attggggttt tgatgataaa aagtaatgat
    68701 aaatctgaaa atctaaaaaa gcataatcga ttgtgagaat aataatagct aatgtcagtg
    68761 cagaaataaa aggtgttata atttttttaa agtctaggat atctaaaaat gaaattgtaa
    68821 tatatacaga taagagcaag aaaataaaaa caggattaat aaatatcaat aaaaatatta
    68881 ttaaaccata aaaatatagt tcttttagag agaaattatt ttttttaagt aaatataatt
    68941 gaaaagtaaa ccaaatatat gaaataatag tactctcaaa aaaggtatca aaatttataa
    69001 atattaaaaa aaatgcaaaa ctgggtaaca tagctattat atagctaaat gaatatttat
    69061 attttttgtc gtaaataagt ataaatgaaa aaatagtaat gacgacaaat aataaaaaag
    69121 ctgagatagc tatagaattt ataaatctaa atttatagat aatagccgaa gtattcataa
    69181 gtatatatga aacaataata tatgtagata aactcaaaaa taaaataagt ttatctgata
    69241 cttttagaca gatattgcta tgtttacctt ttcgttttat catttaatct taatattaaa
    69301 aaaatgaatt cataaatata aatatttaaa taaataaaat tttaaatctt tttttaaaaa
    69361 aaaatggaac tatatttttt taaaaaacaa aattaataat ttaaaacaga aaaatattta
    69421 gattaagaaa aataagaatt taattttata taaatattca aaaaaactgt ttcttgcact
    69481 ttattttagg tttgattaat aaggcaggtt atttgcaaga tcactatgtc aaaaaaaaat
    69541 ttatagttaa tcgagtaatg gttatctgtt attattattt taagtgatta attaaattta
    69601 gttgaaagac ttgaataatg ccttgtgaat agttatttag ctgttttatg ttaaaatatt
    69661 taatttataa tttattatat aaatttgtct aaaattataa gcaattgacg acaaataatt
    69721 ggttaataaa tttgaaaaag atcttaatta aatataaaaa tttaatatta ttagggtgtt
    69781 tttttttgtg catattaata ttattatttt tacctactaa taaaaaacct tattatcagc
    69841 ataaatatca aaaattagac tttccagata taatagaaag aggctatata aatgtaatag
    69901 ttgagcataa tagtatgaat ttctttgtac atcatggtag agtaatgggg ctacaatatg
    69961 agctactcga tcatatagag aaacgatgga atataaatat caaccaaata gtaataaatg
    70021 atctaggcga agcacttgct ttggtagctt tcgatagtat cgatattttg acttcagata
    70081 ttaccattgt acctgggcga tatccctgta tagactttag tataccttta tataaaacta
    70141 agatggtatt ggttctatat gattctataa agccctcgac agagatcaat gatttttcta
    70201 agttagatat taaaaaatta gaaaataaaa taatagcagt tcctactaaa tcgatattta
    70261 tcaatttttt aaataaatta aaaaaaagtt taaatttaaa ttttcaaata aaagaattga
    70321 tagaagaacc tgaatctata ttggatagtg tgatggcaaa gaaatatgat gctactataa
    70381 tggatgaaaa gatattaata gtcaatacac ctttactaaa aaatacaagc tcatattatt
    70441 taccttttcc atccttagat gtaggctggg catttagttg tcatcaacca atattaagag
    70501 atagtgtaaa tgctgcatta atagacttaa aaaacaaagg tattttagct tcattactta
    70561 ataaatatca aagacctata tatatacaga ctacatataa gcaaatagct cattctaaaa
    70621 gaacaaaaaa attgacaaaa tatgataata tcttacaaaa atatgctaaa atttatgata
    70681 ttgactggcg tttagtagca gcattgatat atcatgagtc aaattttaat atagatgcag
    70741 tttcgtataa aggtgctttt ggcatcatac agataatgcc acagacagca gaggaatatg
    70801 gtatgtcgcc tactagctct atagatcaac aaataaaagt tgcatatagg attttaaatg
    70861 attacaaaaa acaattaagc atatattttg ataatgataa agatttatat agagctatga
    70921 tagcttctta caatttagga cttggacata tattagatgc tattgcttta gctctaaaat
    70981 atgatggaca aattaatagt tgggagcaag tagcacaatg gctattgaat aaatcacaaa
    71041 aaatatatta ttctgatcca gtggtaaaaa acggctattg caaaggacga ctaacggttt
    71101 attttgttaa tttagtgtat gaaacttatg aaaattataa acaattaatt taaatatgta
    71161 attttgtcaa aaaaaatgaa tctagagttt tatataccag aaataataca aaaagaagct
    71221 gaagctatta aaaacattcc aatagataat aaatatctgg atactgtaga tctaatatat
    71281 gatcgtgttc atttaaaaaa aggcaaatta gtaacttcgg gtatgggcaa agctggacag
    71341 atagctttga atatagcgac tacttttagc agtacaggta cgccatctat ttttttgcat
    71401 cctgcagatg ctcaacatgg tgatctaggt gtgatacaac ctaatgatgt tttattatta
    71461 atttctaatt ctggcaaaac aactgaaatt ttgcaattag tagatttagc aagaaattta
    71521 tataatgata ttcctttaat agtgattaca ggtaataaag atggtatatt agctcataaa
    71581 gctgatctga ctttatacac tggaaatcca ccagaagtat gcccattagg gctgacgcct
    71641 actacttcta ctactgttat gactgttata ggtgatatac tggtaacatt aatgatgcga
    71701 aaaattgact ttaaagtaga agaatatgct aaaagacatc attcaggata tcttggcgaa
    71761 aaatctaaaa tgtttgtaaa tcaaaataaa aaataataat tttatgtggc tatttgcagg
    71821 tccttgtgta atagaaaatg ataaaatacc ttttgtggta gcagaaaaaa taaagaatat
    71881 tgcagatgag ttaggttatc attttatctt taaaagtagc tatctgaaag ctaatcgttc
    71941 taaattagac agcttcagag gtatacctcg actagaagca ctaaaaatat tgaatgaaat
    72001 aagaaaaaat tataaaatac ctacgataac agatgtacac gaaagctggg aatgcgaaga
    72061 ggtagccgaa tatgtcgatt atttacaaat ccccgctttt ctatgtagac aaactgaatt
    72121 attaatatcg gctggcaaaa cagggaaacc aattaatatc aaaaaaggtc aatttatgtc
    72181 tccagaaagc atgaaatatg ctgtagataa agttaaaagc actggtaatg aaaatatttt
    72241 tcttaccgaa agaggagtta tgtttggtta ttatgatctc atagtagata tgacctcgat
    72301 acctcgcatg aaaagtattg gatgtcctgt attaatagat gctactcacg gtattcaaat
    72361 gccaaatcgt agtgatggtg ttacgcatgg acatagagaa tttgtggaaa ccatagctct
    72421 ggcgggtatc gctgctgggg cagatggcat ttttttagaa acacatccta atcccgaaca
    72481 agctcttagt gatgccgata ctcaatggcc gctagatgat gtttatgatt tactcaaaaa
    72541 agcaaaagaa atttattcaa ttattaataa tgtttaatat gaaaaaattg tttttgtcgc
    72601 tattaatttt aatttatcct ttttttgttt ataatcagat ttttatcact cctgagcccc
    72661 aatcaatgac taaaaggata gatatttttg taggtcaagg ctatgatatt aaatatcaca
    72721 aattgtatat cagtgtaact cctggctctg atcatattag tggtggtgtg tttacaaagt
    72781 ttataccatt agatgatact gatactatgg atttcgatgc taatcagtgt ttagttatcg
    72841 attcagttat atatcatggt aatgctatga cttttattca tcaaaataat aggttaatta
    72901 ttaaaaatct tacactagta tctcaatcat tagatagtat tattatttac tatcatggca
    72961 ctccagcggc tagtggtttt ggatcttttt attctgactc acaatggttg tggactcttt
    73021 cagaaccttt tggggcttca gactggtggc cttgcaaaaa tgacttaagt gataaaatcg
    73081 attctataga tattttcata gaacatccat caaattatat cgctgttgct aatggattag
    73141 agcaatcaaa agtagattta gtgaatagca gaaccttaac tcattggaag cataaatatc
    73201 cggtagctac ttatctaata gctatagctg tagccccata tcattatttt aatgttcata
    73261 tacctctcac tgaaggagat ttacaaattg ataattatgt gctttcatca gcttataata
    73321 atgcacaaaa tgcaatgcct aatttctcaa aggtgatgaa gttttatgat tcacttatag
    73381 caccttatcc atttatgaat gagagatatg gtcatgcaca cttcggttgg ggaggcggta
    73441 tggagcatca aacaataagt tttgtaggga gtttcgatta tgaactttta gctcatgaac
    73501 ttttacatca atggtatggc gatgctgtta cttgtggctc ttggcatgat gtatggctaa
    73561 atgaagggtt tgctacttat ttcaccgcac tttctcaaga aaaatttttc cctcaagatt
    73621 tcatttattg gaaaaatcaa tctattgatt ttattaccta tcttccttgc ggagctgtgt
    73681 atgtttatga tactacttct gtggatagaa tatttaattc tcgtctaagc tacagaaaag
    73741 gcgcttatat attacatatg cttagatgga ttctaggtga tgatgttttt tttaatggag
    73801 tgaggcaata ttatcaaacg tataaatata attctgctaa aactgaagat ttcaaaaaaa
    73861 taatggagca ggtatctgat actacattaa cagaattttt taatgactgg atatacgatg
    73921 agggatatcc gatgtataat tttacagtca ctaatattga tgatgataat ttcaaaatcc
    73981 aagtccatca atcatcatca tgtcattata atacattttt tgaaatgcct ttaccttttt
    74041 tattgcggta tgatattttt gataccatca taatacttaa taatttagta caaaatcaag
    74101 atttttttat caatttacca atgcctaatg aaataatatt tgatcctaat aaatggattt
    74161 taactaaaaa tgcaactatt attatagatg tcgagactta tttaaacaag caattatcta
    74221 tatatccaaa tcctgcatct aataaagtag caataaataa tcatagcaat aataaattta
    74281 ctcttgtcat taccgatcta ttatccaaaa aattaatcgt tgatgattgc atattgccaa
    74341 attctactta tactgtagat attagttcat tgatcactgg cacatattta atgacattca
    74401 aatctgaaaa aaatgaaatt attactaaaa agctagtgaa acaataattt gaaaaattag
    74461 ttttacggtt ttagtatagt tttattaaaa tcaatacacc tacaaagcaa aaattttgct
    74521 acagcaaatt attgacaatt aaatttttat ttaagtaaat gtgattttat ggtataattt
    74581 ttgctaaatt ttaaat
//
LOCUS       DAOPRN010000002        62294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_352_length_62294_cov_126.536271, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000002 DAOPRN010000000
VERSION     DAOPRN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 62294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62294
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_352_length_62294_cov_126.536271"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..1430)
                     /locus_tag="PKI65_00335"
     CDS             complement(276..1430)
                     /locus_tag="PKI65_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOB76927.1"
                     /translation="MRLIFVIILILNINFLLAQDFYPEECENLVPNASFEGNGKTTLK
                     PWKKLNTADYFVYSTSKKIQDVNKIKFDKNYILRPARTGNAYAGLRFWPKNYNEFLQV
                     KLNKTLEKGKIYYFEVYITPSKYSNCYAKSFGASFYPNAPNYLSPLVLETYRPQIEVR
                     DEKGLKSDDPENEWLKISGTFTAMGNELFLTIGNFDLNNRDKFKRKSIFSFGKREAYY
                     YVDDVLLCEIGIDSTLYFLSLNTTLDSQLFDIDEIDISAKDIIKTPNREDTLVQLIPD
                     EGGSKTFFIYFEPNSYKLNDTIYRFFSYVVEVLRENPSLKILIKGYQETNESDDIKLA
                     EKRAMNIFYLLNGNGISQSRFTIQLPKQSCKSDFGALLHCRLVTITIINE"
     gene            complement(1432..2094)
                     /locus_tag="PKI65_00340"
     CDS             complement(1432..2094)
                     /locus_tag="PKI65_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76928.1"
                     /translation="MKRIAIIIFTAYILVFCVNINAQTTKHICDNVNCTGNCGKYIDD
                     DQDGYCDFGIKLENKNIDTISQNMTLSSVDDVPNDNGINNNFIHKTIQQYLVNYQNHF
                     NVLPVLRLNIKKRSYNLIEIGATTIILYLLSWILSKNKVISKRLHKRIWNLLLLISFL
                     VSCLLGILLIIQINYDFPHKVLYNDFLYWHVQIGIVMVLIGIFHALWHIPYFKKIFSP
                     IE"
     gene            2248..3201
                     /locus_tag="PKI65_00345"
     CDS             2248..3201
                     /locus_tag="PKI65_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOB76929.1"
                     /translation="MEKILVIPHPIEISEKQIRFLKDELSKYNVKLVYYTDEPSNDEE
                     LINRISNANYLIIAVLPLNADILSKAKNLEYISITFTGYDHVDINYCKANNIKVSNVA
                     GYSTTAVAELTIAMAIMLYRQLIPQYLSFKLSGDKINKPGREIYNKTWGVIGYGNIGK
                     KVTEYAKSLGANIIVHTRSLHPEASNIDFVSKQHLIANSDIISIHMPLNNETKHFISF
                     REFDQMKDNTILINTARGLIVNQEALIDALINKKIAGAASDVFEIEPPLDPQYPLLHI
                     DNFLALPHIGYYTEEALEKRADIGIDNFLNYLNGKITNEVC"
     gene            3655..4761
                     /gene="meaB"
                     /locus_tag="PKI65_00350"
     CDS             3655..4761
                     /gene="meaB"
                     /locus_tag="PKI65_00350"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOB76930.1"
                     /translation="MLTEDNKNNKESALKINEGIEQPSQVNQEVINKFLSKTKTPSTQ
                     ELIEGIKNGDRVALSRAITLIESLNPRHYCEAQKIIEACLPLSGNSIRIGVTGIPGAG
                     KSTFIESFGTMLTELDYKVAVLAIDPSSQLSKGSILGDKVRMERLATNPKAFIRPSPS
                     AGTLGGVSRKTKETMILCEAAGFDIVLIETVGVGQNEVAVRNMVDFFLLLLVAGAGDE
                     LQGMKRGIIEMADAIFINKADGDNINKALASAQEYKLALHLLPPHPAGWTPIVDVCSA
                     ITGYNLDKVWETIQKYINLVKQNAYFEKNRQNQEAQRIYDYLIEELNMFFWNNEKLQN
                     EYNKLKDLVLKQKITAYQAAIKLKKLFIKLISKR"
     gene            complement(4774..5403)
                     /locus_tag="PKI65_00355"
     CDS             complement(4774..5403)
                     /locus_tag="PKI65_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76931.1"
                     /translation="MKRNYLSILFIFFQYIFLFGVNNSSDSLVLAWAKDDPTLSKILS
                     VLPSNWHFELDKPYLKIYCEDSLYCKNKNIESDSLESVITDKVQVFILLKFEPIWSIE
                     KILSAKQNNERVNKEIIELQHKYNKKIIMESKNQAYKPTTYLDSVYYQKLKIINNKYI
                     ELPNFNTIKYSWFFVDECCTTNEIYDYHPMELMIQMLNIKNLFRELGGS"
     gene            5625..6098
                     /locus_tag="PKI65_00360"
     CDS             5625..6098
                     /locus_tag="PKI65_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOB76932.1"
                     /translation="MKTQEKNNEKMLNEVENGQVEEFSEDLCLDENAFAEKLAGIISE
                     QRSVNMLLLKGFIASDVQVFNYGTWEKYSFTLGVSERKWNGANFEYYNQYLPITLNGV
                     LADIVGKQYKKGNEIIVLATIHRRVKEIDGKRRAFYDIKANDLYAVNKSQSAKVA"
     gene            6359..6925
                     /gene="efp"
                     /locus_tag="PKI65_00365"
     CDS             6359..6925
                     /gene="efp"
                     /locus_tag="PKI65_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOB76933.1"
                     /translation="MATTSDISNGICIEFNGELYTVVEFQHVKPGKGAAFVRTKLKHI
                     PSGKIIQNTFASGAKINIQRIERRPYQFLYKENEYYHFMHTETFEQIMISESLINAPQ
                     FLKEGQEVEIIFHANSETPLLCELPTYVVLEVTYSEPGVRGDTATNAMKLAILETGVE
                     IKVPLFINVGDKIKVNTQTGEYGERVNN"
     gene            6956..7963
                     /locus_tag="PKI65_00370"
     CDS             6956..7963
                     /locus_tag="PKI65_00370"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOB76934.1"
                     /translation="MTLDRSYSIQEIISIIDNPLAKVIGDTSIKATGINEIHRVNPGD
                     ITFVDNEKYFNRVLSCDASIIILNKEIEAPDGKVLIYSDDPFRDYVKLVKFFAPFIPQ
                     DMAIHPSAEIGKGTIIQPLVFIGPNVKIGENCIIHPNVSIHANSIIGNNVIINSGSVI
                     GGEAFYFKRRPDYLDKLEGCGRVVIEDNVEIGSCCTIDRGVSADTIIGKGTKLDNLIQ
                     VGHDTIIGKNCLIAAQAGIAGVVNIEDNVILWGQVGVQKDLTIGKGAVVLGQSGIAKS
                     IEPGKVYFGSPAREASDKMREIALLKKLPELFEIVNNLMRIHGIDSDLKINNKNLNNF
                     E"
     gene            complement(8049..9113)
                     /locus_tag="PKI65_00375"
     CDS             complement(8049..9113)
                     /locus_tag="PKI65_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76935.1"
                     /translation="MKKLNLIIFLVFALILISCNNDEPNETPVNPDNDVSFITDGVLI
                     INEGLFGQGNGEISYYDFSTNKVSHQIFYNINNKLLGDLPYRLAVSDDVAIITVNNSN
                     KVYIVNLHNFLLLSEYDVPSPREIVFRDLRHIYVSSLTESYLYVINSIDNSISKIYCD
                     RPIEQIIEVNSKIYGLAWSNYYSGKANKFVMVIDLVQNQLIDSIEVGIEPQTIVYKDN
                     YLWVLSTGGYMHEENAKISKISLDNHQIVHYWNFPNNNDYPSYLIIRNGKMYFLNNNA
                     IQSATIDDHNILSINTLYAASSSSSLYYLMWDGDDNCFWVSDVKDYMHNGSVYKISEN
                     GELLLTIEAELVPGMICRIL"
     gene            complement(9143..11026)
                     /locus_tag="PKI65_00380"
     CDS             complement(9143..11026)
                     /locus_tag="PKI65_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76936.1"
                     /translation="MINFYKHTIIIIICFIYIIFNSDYSCCQDTIYLQKIEIIDSTQK
                     MGRLSTFINSFSISDSMHFIPIAERQWLQIIKMQTPVYLKEYGVQNASISFRGFGAGH
                     TLLIWNDIPLLSYSLGQSLWLSFFSNNFRINNYEGAFSSLGFPGALGSIIEAETVFAG
                     KNHYYIGYSFATINNNDIEFSISQQLKNGNAFRVQYNGSFDKNYYEFINTMHNNKLEV
                     KRDNEHLGQQLSFDIKLNKWQMGFNIQYHQNEVPASILSAQLDNNATQNENIQLAYLR
                     YNWSSSFHWQHYSSFGWYSEQYEYNSNLPPAETVSNSLAPQIYHQSNFTFGDHQLLLR
                     IRFSYQYLNSSAYQKIADRYITDQSLIYNYENNGWQWWVSEQLQIKNFKKYYPQGGIG
                     VSKKWKYGNHNFIVAGALSHLERLPSFNDLYWVPGGNPDLIPEASNNFDIKVKWLFNK
                     GFFYSICEINPFYYFTNNLIQWKPSENQAIWTPININKAIQNGIQANWKLDFTFNNFI
                     LRFTSNFLVQYAEDRETHKELIYVPNYIFNELINLSIKNHWKINYEYSYNSKRFSTFD
                     NDRYLPWYAVQNLGLSYEHNIKKVSMHYSIWVKNLTNANYCTVAWYPMPRRYYIFSLQ
                     IEL"
     regulatory      complement(11082..11288)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(11588..12574)
                     /locus_tag="PKI65_00385"
     CDS             complement(11588..12574)
                     /locus_tag="PKI65_00385"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB76937.1"
                     /translation="MDNFNLLDYQALPKEQKRRFLDNLYQFLLKNNYTAVDYQKLKIQ
                     STAPICPRCESENVIKAGVRDGKQVYKCKDCGRQYRETARTFVYRMRKADKMLDYLKC
                     MLEGKSLRACASEVGISLRTSFNWRHKILAAIKSLQGGISFSGIVEADELLMKYSEKG
                     RKYRSRKEYEKAKKKKHPKVAVLVMTDREGNLLFKHVGRKKVTNSQIREELSHRVTDS
                     NLICVKQKKEFKKAVKGTKSKKVVVNKTQIRRGVYSVQVVDKHINDFKVWLQRFRGVA
                     TKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSDRWAWDEYWRLVNTKYR"
     gene            12808..15396
                     /gene="mutS"
                     /locus_tag="PKI65_00390"
     CDS             12808..15396
                     /gene="mutS"
                     /locus_tag="PKI65_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOB76938.1"
                     /translation="MAKKLTPLMKQYWEIKSQYPDTILLFRIGDFYETFGEDARIASQ
                     VLGIVLTKRHSGAAADEDLAGFPHHSLDTYLPRLVRAGYRVAICEQLEDPKLAKTIVK
                     RGVTELVTPGVVFTDNVLMQKENNFLAAYYYEKDLHGIALLDVSTGDFFATQGDKDFI
                     KRILESHHPTEVLLSKEQKEYFLDDLGDYPVFTFDSWIFQYDFARDKLIEQFETENLK
                     GFGLEDMPLAQICAGVSLYYVSSTQQRQLSNITSISRIDNNDYMWLDNFTIKNLELLE
                     PLHNEGISFRKIIDHCVTPMGSRLLSRWIIMPIIDINIINWRLNIVDFFVNNNEKLQL
                     IRENLSSIGDIERMVGRLASQKINPKELLQLANAIDVAFNIQKILDDVPDFFNNKKKI
                     DHKLINIVNKIRNTIVEEPPASIQKGDCIKDGINVELDELRNIKNHSKEILKEILQRE
                     IKNTGIASLKIGFNNIFGYYFEVTNPHKNKVPASWVRKQTLTQAERYTTEELKTYEDK
                     IISAETRIMQLELEEYDILLDGLIPYCRDLLYAGHLIAELDVLTNFAHIAIINDYKRP
                     VLTDDLIIDIDQGRHPVIEKMLKAGERYIPNDLYLDTNNQQIIVLTGPNMAGKSAFLR
                     QTALIVIMAQMGSFVPANKAIIGIVDKLFTRVGASDNISLGESTFMMEMNETASILNN
                     LSERSLILLDEIGRGTSTFDGISIAMAIIEYLHNHPYYRPKTIFATHYHELNEVANYL
                     ERVKNFHFSVKEIDNKIIFLRKLEEGGTEHSFGIHVARMAGIPNIVVNRANNILKQLE
                     ADRENNKWEEKPLNKGIQLSLFQLDDPLLEELRQMILSLDLDQLTPLEALMQLNLIKS
                     KLKSSK"
     gene            complement(15490..16389)
                     /locus_tag="PKI65_00395"
     CDS             complement(15490..16389)
                     /locus_tag="PKI65_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOB76939.1"
                     /translation="MKNFWFKFFHLSISEWRGIFLLAILIFFISLLPYTLKNHYKPTA
                     LNFDDLDSISILLQNNRILALASRDSLFQTNYKTWGVNSINPFPFDPNTISANDFKKL
                     GFTDKQTQTFINYRSKGATFKTKNDLLKLYFVDDDIFAVLAPYILLPDTITYSNVKTT
                     KKTDKAKVQIELNAADSIDLIKIPGIGKYRAKKILELKNKLGGFIDYDQLKMIYGFND
                     SLIETLKPYLTINPYLIKKININNANIKEMAKHPYIGYNLAISIYNYKQNHGNYKSIE
                     ELKNLKSVNDSIYHLVLPYITVQ"
     gene            complement(16386..16784)
                     /locus_tag="PKI65_00400"
     CDS             complement(16386..16784)
                     /locus_tag="PKI65_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB76940.1"
                     /translation="MKKIFSILIIIFSVSIVMCNAQTITDTIDFNPDYSEPLVIMPNA
                     FTPNGDGINDYFYPFISYNYPIVSYELRIYGLNGQEVFYSTDPFTRWGGDKSDMETIH
                     NNTKTFVYYLNVLFSDGARKRLRGSFLLLL"
     gene            complement(16838..17689)
                     /gene="nadC"
                     /locus_tag="PKI65_00405"
     CDS             complement(16838..17689)
                     /gene="nadC"
                     /locus_tag="PKI65_00405"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOB76941.1"
                     /translation="MNRLSIDDIIKWALIEDIGDGDHTSIAIIDPQAVGNTALYAKEN
                     GVIAGISFAKRLIHYYDPNISIKLLKSDGDAIVAGDIIFMLYGKQLSILSIERVLLNF
                     MQRMSGIATTTRAMVDQISHTRAKLLDTRKTTPLLRTLEKEAVRLGGGYNHRFGLDDM
                     ILIKDNHIDFAGGISQAIKKVEIYLSKNNLKLPIEVEARNIDDVKEILNYDSVLRIML
                     DNFSVDDVKLAVSIVNGKKELEVSGGITLKNIKVYAETGVDYISSGYMTHHYGSIDLS
                     LKVIKGT"
     gene            complement(17713..18504)
                     /locus_tag="PKI65_00410"
     CDS             complement(17713..18504)
                     /locus_tag="PKI65_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOB76942.1"
                     /translation="MKKNLIWSLYQANTLWMDIDGNLKNIETVIKKSKSFDADILVCP
                     EVCTTAYATKNPEIAQTMDSFMIDRLLSMAKKYQLAICGSFMIQENGKLYNRFIWFSA
                     DGEIFKYDKAHLFSLADENIYFTRGSNTITISYQGWEIRPYICYDIRFPVWNRNKKTD
                     NKFDYDILVYVSNWPTKRYYHWKHLLIARAIENQAYVIGVNRVGKDGNGFEYSGGTMC
                     IDPAGSVVLEAKQDENIFIVNTYYDHLTKIREKLPFSSDWDNFKI"
     gene            complement(18644..21736)
                     /gene="secDF"
                     /locus_tag="PKI65_00415"
     CDS             complement(18644..21736)
                     /gene="secDF"
                     /locus_tag="PKI65_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOB76943.1"
                     /translation="MRLKGTVWFFVILFLLASIFSLSFTLCTRKVENKAKEFAYSEAT
                     YKEAEKLAMGDSIKMMMLLDSIAREKQSQYLDSMMDVKVYNILIKKYTYAECKEKELQ
                     LGLDLKGGMNLTIEVDDAAIIRALAAKKNDPKLEEALKRAQERKVASNVSFVDLFVEE
                     IKKIDPNQPLVTYFLSRETSDYLNVNSTDQDVINYLKREIKSAFDNTFIVIRKRIDKF
                     GVTQPNIQKLVGQSRILIELPGVDNPNRVRSILQTTAKLEFWETWNLTEIAPRLEQAN
                     KRLAQMLKSQDSLLFVEDTATIVESKPKKDTLKDTSKLAQQLKALETKPLGETPEDIE
                     FEQYKKENPLFAYLTINIRQEGNQQFYGEGPLIGYAYAKDTAKINEYFRLTQDLFRDV
                     KFLWSAKPIANTRNVYELIALKVNPLTNKAPLEGDVIVNARQDVDQYGNVIVDMQMNS
                     EGAKIWKDLTAKNIGRSIAIVLDNLVYSYPVVQSEIPNGRSQISGNFTIEEAQDLATV
                     LNAGKLPAPARIIEENVVGPSLGSEAIKSTMIAFLIAFILVIIYMITFYGTAGIGASI
                     ALLLNLIILFGVLASIGATLTLPGIAGIVLTLGMAVDANVIINERIKEEVRAGKGLRL
                     AIKDGYKNSYSAIIDGNVTTIIAGVVLLIFGMGPVQGFATTLVIGILTSLFTAIFVTR
                     LFIELLLDKGKNVTFYHKFSENFLANTKIDFITKYKTFFIISGIVILASIVSLSTKGL
                     QLGIDYSGGRAYVIRFDQPVKATEVQSSLFKVLNEVPEVKIYGNNNQVRIVTKYMLED
                     TSRNVGAIIEKALYDGVKEFYKNPVSFEDFSTDNPNKVIGIMSSQNVGPSIAKDIQRS
                     AFISIAIALLAMFIYIMIRFKKWQYSLGATVALFHDAFIVIGFFSIFYGILPFNLEID
                     QNFLAAILTLIGYSINDTVVIFDRIREYNILFPKRPLKQNMNEAINATLSRTINTAGT
                     TIVVLLAIFLLGSEVIQGFTFAMILGVIIGTYSSIFIASSVAWLIMDGGKKSKKIEKK
                     "
     gene            21866..22236
                     /gene="ssrA"
                     /locus_tag="PKI65_00420"
     tmRNA           21866..22236
                     /gene="ssrA"
                     /locus_tag="PKI65_00420"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            22884..23909
                     /locus_tag="PKI65_00425"
     CDS             22884..23909
                     /locus_tag="PKI65_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76944.1"
                     /translation="MKKLFLISILIVISMTMYGQKAALHTASGVQIFNGSNAFVNAHN
                     AASSGDTIYLSGGTFYAPTSITKTLYIYGAGHYLDSTQATGKTIISGNLILDRNTDGT
                     YIEGIDLTGEVRLQFSTPDTISNITLAYCKISGNINLSYNGNTYSNNFALQRSVVIGS
                     INFTRATNAAVFNSILQSQCNSSNGALYENNILFFAIASYSSGFTIIGDNNIIKNNIF
                     FKNDNKSGVGGNGNQFFNNLFTTATPYFGGSYTASGNYYPVNIDSVFVNNNIYTFDYS
                     KNYHLKHPDTYIGTDGTQIGIYGGTFPYKEGAVPSNPHIQLKNVAPTSDSNGQLQIEF
                     KISAQDE"
     gene            23902..25674
                     /locus_tag="PKI65_00430"
     CDS             23902..25674
                     /locus_tag="PKI65_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOB76945.1"
                     /translation="MSNPSYFFSTILKTLSVLLFLAMSQSIYAQQTIVEYEYWFNDDF
                     NNRTITAITPVSQYHLDTMISVSTLPNGINVINFRAKDSQGKYSVITSQLFYKKELTD
                     SDTNHLLSYEYWFDDDFQNKTVVDISPAEQTTVLAQLDVSTMSNGMHLFNFRAKDERG
                     YYSSIAYQFFFKKTVTSSDTNYIVSYKYWYDDDYNNLIYVDLPTPTKQLNLIDNLDMT
                     RIPKGIHTINFQFKDDANFWSVISTDTIEKISLPIADYTYEVLLSNCDSTVIAFTDNS
                     IDGDMYLWDFADGTTDTLANPTHTFYNSGIHSVILTITDTTTLADSTKQMDILITGHT
                     ANSFAVMNCDSYTSPSGNYTYTTSGVYYDTIPNHWGCDSLLTINLTINYSDNITDVIT
                     ACDSYTWIDGNTYTASNNTATFTLTNAAGCDSIVSLDLTINYTPDNTITQMGDTLISN
                     ADGVNYQWLDCNNDFSPIFGENNQNFTPTETGNYAVEVTNYECIDTSDCINVIIDNIL
                     ENTFAGKIKIYPNPSDGSFYIDLENYYDKISISLANVIGEIVYQNNYYNTRLILINIE
                     NAKGIYFISISSADEIATFKIILN"
     gene            25900..26583
                     /locus_tag="PKI65_00435"
     CDS             25900..26583
                     /locus_tag="PKI65_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOB76946.1"
                     /translation="MNLPVNINYKEFFDFIKQFLTDNRLERFYNVINDRITNVQIILE
                     DIYQGHNASAIIRTCDAMGLQYINVIEKRNKFKPNKDISLGSDKWVDIMTYSGEKGLN
                     DCIKVLKQRDFTIVATSSHLPEAYTPETLPIDKPIALIFGVEKEGLSDEAMSLANEYM
                     YIPMYGFAESLNVSVSVGISLFSITHRIRKADKMLKPCGDEYYKILCKWALATLSNSE
                     FYIKTFINK"
     gene            26588..27802
                     /locus_tag="PKI65_00440"
     CDS             26588..27802
                     /locus_tag="PKI65_00440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOB76947.1"
                     /translation="MPNNKVRNKAFTFLLLMGIVSLLADMTYESARSLIGPYLLFLGA
                     SASVLGFVFGLGEFIGYTFRLVFGFVVDKTKNYWLFTILGYSINLIAIPLLAIVPPNG
                     WIWAAILIICERFGKAVRAPAKTTLVSFAAKRVGTGKGFGFVEVLDQIGAMLGPLFLF
                     FIMQWKESAGTHAQYQWAFASLAVPAVLTLIVLFIARAIFPKAEKFELTDKKTNITSQ
                     KLKFKEIKPILLYIIAIGLLAMGMADFPLMAFHIETNKVIDVKYITLIYMIVMGVDGL
                     SAFIFGNLYDKKGAMTLLISAIIGLLFAPLVFVSTNLTSIVIGLILWGVLLGAMESVA
                     LAYIADNVEVAYRGRILGIYYTFYGITWFVGSWLMGILYNVNPVYVAIFSIVILLSAI
                     IVLWRQIKINKQ"
     gene            27799..28272
                     /locus_tag="PKI65_00445"
     CDS             27799..28272
                     /locus_tag="PKI65_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76948.1"
                     /translation="MRIFCDLDGVLTDFDGRFFELFGKYPNEFIDTYGYSSFWASFTR
                     QAEDFWSGIKWKNDGKKLWQFIRPYNPIILSSVPPSMAKIATKYKEKWIRDELGLDIE
                     YYITSRKEKQNYSDQGSILIDDMKKNIDEWLLRGGIGILHISAENTIKELKKIGL"
     gene            complement(28279..29220)
                     /locus_tag="PKI65_00450"
     CDS             complement(28279..29220)
                     /locus_tag="PKI65_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76949.1"
                     /translation="MTKALLLFSGGLDSILAGKLLKEQGIYVTALKYITPFFGWELKK
                     EPEKFLKKIEDYEFDEGKIIDITEKYLKLLKKPKYGYGSHANPCIDCKILMINEACEL
                     LKEEKADFIATGEVIGQRPMSQTKNALVLIKQKSKAEDLLLRPLSAKLLFPTKPERDG
                     LVDRDKLLAISGRSRKEQLMLAEKYGIKDIPSPAGGCLLADPVIGDRILKVLKGPFDF
                     NPITAELITFGRHFIRDDTWIMIGRDYEENKKIYEIAYSNYDIYCLSEPSPVGIVLKG
                     NKDILKDLLLKYSKKAKLAIKEGENVDLNIIKNIDEV"
     gene            complement(29807..30325)
                     /locus_tag="PKI65_00455"
     CDS             complement(29807..30325)
                     /locus_tag="PKI65_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOB76950.1"
                     /translation="MNYRKKELVPILNDQLQIINVANRDEVHNGSKMLHPVVHLYVFN
                     NEGNLLLQKRSNKKEIQPGKWDASVGGHIAYNEKPEDALFREAKEEIGLKKFIPQLIH
                     HYIWESQIEKELVFVFVTKILDQNQLKLGKEVNELRWWTKKEIFSQLGKQFFTPNLEY
                     EFLNILDKIWKS"
     gene            complement(30362..30868)
                     /locus_tag="PKI65_00460"
                     /pseudo
     CDS             complement(30362..30868)
                     /locus_tag="PKI65_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
     gene            complement(30880..30952)
                     /locus_tag="PKI65_00465"
     tRNA            complement(30880..30952)
                     /locus_tag="PKI65_00465"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30917..30919),aa:Gly,seq:ccc)
     gene            31031..31942
                     /locus_tag="PKI65_00470"
     CDS             31031..31942
                     /locus_tag="PKI65_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOB76951.1"
                     /translation="MNFTKSPDWQREHNFYKEKKTAEQKTLIVIIITFITMLAEIITG
                     WIFGSMALFSDGLHMGTHVSALSISLFSYILARKYAKDIRYTFGTWKFEILGAYTSAL
                     ILGLMGLFVLIVSIKRFFNPVKISYDYALLVAVIGLLVNVVCAFILRDNNKHEHNKDL
                     NLKSAYLHVIVDALTSILAIIALLGAKYFNLNFLDPLMGFVSSFLIFRWTYFLIKDSS
                     AILLDKQTNPTLVENIIQIIESDGKSKVIDLHLWKLTSDKYACIVSIDTSGLYSIYEY
                     KDRLKKFEELAHINVELHKNNGNLRTS"
     gene            32101..33231
                     /locus_tag="PKI65_00475"
     CDS             32101..33231
                     /locus_tag="PKI65_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOB76952.1"
                     /translation="MRIAVNTRFLLYQRLSGFGVFIEEIFSRLVKEHPEVEFTFIFDR
                     PYHSSFIYNTNVTPKVLYPPARHPILKYIYYQISLKRYINNNKFDLFISPDSELPLHL
                     KCPSLLVLHDLNFMYDHKYLPLSDRRYMLYFTPKFLKHATRIITVSEFSKHDIEHNFP
                     FTNNKVGIVYNAASEKFKPLSEQKIQKFREKYSKGKPYFLFVGVITPRKNLANLLKAY
                     SIFREMSDIDYPLIIGGNKIHKYKEFEKELKNNKYSSDIFLIGKVLDEEIASLIGSAY
                     SLVFPSKFEGFGIPIIEAMKSGIPVITSNVSSMPEVAGDAALLVDPYNPRSIADAMLL
                     LTKSNDLYVDLKNKGLERAKSFSWDISAKKMWEEIEKTIGIS"
     gene            complement(33338..33928)
                     /gene="rdgB"
                     /locus_tag="PKI65_00480"
     CDS             complement(33338..33928)
                     /gene="rdgB"
                     /locus_tag="PKI65_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOB76953.1"
                     /translation="MELIFATHNLEKLKEIQNLIGSNIKILSLNDIGYSREINETGQT
                     FEENAFIKANTIYQIYNKPVFADDSGLEVKALNGRPGVFSARFAGEGYNSFDPHIKKL
                     LNELKGIKQREARFRTVICLIINDKVNFFEGIINGHIAEYQSGVNGFGYDPIFIPNGY
                     SKTFAEMNIYEKNKISHRAKAVSKLIKFIMSDPHLF"
     gene            complement(34039..35076)
                     /gene="ruvB"
                     /locus_tag="PKI65_00485"
     CDS             complement(34039..35076)
                     /gene="ruvB"
                     /locus_tag="PKI65_00485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOB76954.1"
                     /translation="MSRNDALSIDKNQLSEKDREYESLIRPKNFEEFFGQTQIIENLR
                     VFVKAAKARNEALDHVLLHGPPGLGKTTLSYIIANELGVDIKVTSGPVLEKPGDLAGL
                     LTSMKPFDVLFIDEIHRLSPVVEEYLYSAMEDYKIDILIESGPNAKSIQIKLNPFTLI
                     GATTRSGLLTSPLRSRFGINSRLDYYTSDVLLQIVLRSAKILNVEININAAEEIARRS
                     RGTPRIANLLLRRVRDFAQIKGSGIIEKQITLYALNALNIDSHGLDEMDIKILTTIIQ
                     KFSGGPVGLNTIATAVSEDPGTIEEVYEPFLIQQGYIKRTPRGREATKLAYEHLGIKY
                     KGSDDTMSLFD"
     gene            complement(35139..35576)
                     /locus_tag="PKI65_00490"
     CDS             complement(35139..35576)
                     /locus_tag="PKI65_00490"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOB76955.1"
                     /translation="MISRDEYFMREALKEAQKAFEYGEVPIGAIIVANNKIISKAHNE
                     VERLKDPTAHAEMIAIATALEFFNNKYLDHCTLYVTIEPCPMCAGALSLTRISKIIYA
                     ASDIKYGYTLFHPSILHKKTEVKNGILKDDAIKLMKSFFENKR"
     gene            complement(35581..36762)
                     /locus_tag="PKI65_00495"
     CDS             complement(35581..36762)
                     /locus_tag="PKI65_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOB76956.1"
                     /translation="MKKLITCLIIFISYFLIAQKNLLPNGDFEIISRCPNSHTKYLNV
                     DTNDIFLPFWYFINRSTPDFFHRCAKGTNVSVPCNFAGCSEPKSGDGFVGIILRVDTA
                     SYQYNKGYVEHISTEIAISLKKNSYYALSFSYKSAPNSGIITNGLGVYLSSSLPDFNE
                     YNEYIFYKPQIFLHDSIIIDNQEWMDFVGFFIASGDEKYITIGNFVPYNLQNTKIIRK
                     NKSINEMDYYAYCFIDNVKLYELKNPNDLIINTICTQTDSFLEVSLITKTNIWKKLDV
                     LPLNQPFVLDNVYFTFDSYELSANSLIYLDSIVDFLKTYQEIEFVINGHTDNIGSDFY
                     NKWLSTERAKAVYKYFFNKGISPARMQYVGYGDKKPISTNKTNEGRQKNRRVEIVLIK
                     K"
     gene            complement(36996..37232)
                     /locus_tag="PKI65_00500"
     CDS             complement(36996..37232)
                     /locus_tag="PKI65_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76957.1"
                     /translation="MYTVYKVVDKHINDFKVWLRRFRGVATKYLQSYLMWFVITHKYL
                     TSEIESDIGRLLNLASSDRWAWDEYWRLVNTKYR"
     gene            complement(37343..38599)
                     /locus_tag="PKI65_00505"
     CDS             complement(37343..38599)
                     /locus_tag="PKI65_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOB76958.1"
                     /translation="MQIKSIKINFPFFYAICFYLIFISNIFIPPFDIGLGIYFRFVDI
                     FVPLSLLLIIIDKNRIIPKSFIFFIVFALWIAICLIVNHSNVSYNDFFEFYKIIKFII
                     ITIITISYFKYFPHHFNLFIQITFILLLIINLFQYFNIFHFHDLIVPYYDPSLLHWST
                     FGKNSLGQPGPKRMLGTAGNPNNNALIFLFYFCWFLWSMFDEKIKKIKYFQWIFLFLS
                     ITAIILCQSRTALVIIIVIPIIYFIFYHKPIKYLICILLGTAFIFFILSITNKYSIIY
                     FSDFFKNIVESGENIVSTNQSVRGRLDVWKFLIDMWKEKPIFGYGPYKNFFYSYTIYS
                     ENEYILYLWRYGIIGIILYIFWYLFPIIKYENKKFKIFITPFYLLFGLSMMIAAITNN
                     PISHPMISTLLAIAMGHHFALRKAPF"
     gene            complement(38717..38807)
                     /locus_tag="PKI65_00510"
     tRNA            complement(38717..38807)
                     /locus_tag="PKI65_00510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38771..38773),aa:Leu,seq:taa)
     gene            complement(38813..38885)
                     /locus_tag="PKI65_00515"
     tRNA            complement(38813..38885)
                     /locus_tag="PKI65_00515"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38850..38852),aa:Gly,seq:gcc)
     gene            complement(38947..40326)
                     /locus_tag="PKI65_00520"
     CDS             complement(38947..40326)
                     /locus_tag="PKI65_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOB76959.1"
                     /translation="MKEIFFEPYKVKMVEPINMSSKQEREQWIKEAHYNLFNLPSERT
                     IIDLLTDSGTGAMSDRQWSALMLGDESYAGASSYYNLKKVIEDILGFPYFMPTHQGRA
                     AENVLFSVLIKSGDIIPGNSHFDTTKGHIEYRQATAIDCTIDEAFDTDIIHPFKGNVD
                     INKLVKVLETNEASKIPFIIVTITNNTAGGQPVSMENLKQVREVANHFNKLVVIDGAR
                     FAENAYFIKTREKGYEDKNIKEIVKEMLSYADIMTMSAKKDGLVNIGGFIAMRDHKIW
                     EQTSTFNVMFEGFVTYGGMAGRDMNALAVGLDESTEFTYLDYRVKQVKLLGDILQESG
                     IPYQKPAGGHAIFVDAKKFLPQVPKEEYIAQTLAVELYVEGGVRGVEIGTLLADRDPI
                     TKQDRYPALELVRLAIPRRIYSNDHLRYVGEVLKAVYEKRDQIKHGYRIVEEAPIMRH
                     FTIKLDKIL"
     gene            complement(40450..41061)
                     /locus_tag="PKI65_00525"
     CDS             complement(40450..41061)
                     /locus_tag="PKI65_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76960.1"
                     /translation="MKRNILVSFIIVFTVLIMISCEEEKVLYPLDQEIKDYTYFPIGT
                     FWIYMDSLSNEKDTLRVIGGEMGTYESTEKDFEYEYFSQFIYSTRKKANMLYMGTAEY
                     YLDYQTCVLNQYGNGPKFFSMKPRGYNYNNLIYEKNLDSLDVGQRCYNNIKVFKYIKT
                     YTDTSDYERYYYVKKIGLIRKDINKTQEQWELIDFYLNIIPQF"
     gene            complement(41131..42426)
                     /locus_tag="PKI65_00530"
     CDS             complement(41131..42426)
                     /locus_tag="PKI65_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOB76961.1"
                     /translation="MSEELKSKRESLGVIGLGYVGLPIALAFAKCVHVIGYDIKPDRV
                     ELLKKHVDPSNELPSEAFDNCDIDFTNNYEDLHNASFYIITVPTPVDKHNFPDLSLLI
                     KASETIGKVIKKGDYVVYESTVYPGATEEDCIPVIENISGLRAGIDFKYGYSPERINP
                     GDPNHTLSNTIKVVSACDKESLEQITTIYSWVVEAGIHQAPTIKVAEAAKIIENTQRD
                     INIALMNELSIIFNKMNINTYDVLEAASTKWNFLKFEPGLVGGHCIGVDPYYLLHKAY
                     EYHYLSQVIMPGRRVNDAMGYYVAEQTAKMLMATGKNILETNVLVLGVTFKENVSDIR
                     NTRIIDMISELKTYGINVDAVDYMADAKEFYKEYGYQLKDIPGNNYDAVILAVKHKNY
                     EKLTEDDLTQLLRNDGKGIVVDIKGILRGKINKFKYWTL"
     gene            complement(42426..43391)
                     /locus_tag="PKI65_00535"
     CDS             complement(42426..43391)
                     /locus_tag="PKI65_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOB76962.1"
                     /translation="MNKFNFAIIGAAGYIAPRHMKAIKDTGNLLTAVLDPYDGIGIID
                     SFAPEADYFKEPERFDRHLDKLRRQGEKEKIHYVSICSPNYLHDAHIRLALRNQANAI
                     CEKPLVLNPWNIDALQIIEKETGYKINTILQLRLHDKIKELKKQIETSVDKRKVDVDL
                     TYITSRGKWYFYSWKGDIQKSGGIATNIGIHFFDMLLWIFGSVKDSYVHFHTRDRAAG
                     YLELEKARVRWFLSINASDLPEQAINKGKRTYRSILLNNEEFEFSEGFTELHTESYKE
                     ILTGKGFTLEDARASIELAHFIRNSTPNAKHGDLHPIANQIIKEL"
     gene            complement(43403..43981)
                     /locus_tag="PKI65_00540"
     CDS             complement(43403..43981)
                     /locus_tag="PKI65_00540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOB76963.1"
                     /translation="MNEEYFSHHTAIVDEGAIIGRGTKIWHFCHVMSSAVIGDNCSLG
                     QNVFVATGVRLGRNVKVQNNVSIYTGVVCEDDVFLGPSMVFTNIINPRSAVVRKDQYQ
                     TTIIRRGATIGANATIVCGIEIGEYAFIGAGAVVTKDVSPYALVVGTPAKQIGWMSEY
                     GHKLFFNNNDIAECPESHQLYQLIDNKVIRIK"
     gene            complement(43985..45121)
                     /locus_tag="PKI65_00545"
     CDS             complement(43985..45121)
                     /locus_tag="PKI65_00545"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOB76964.1"
                     /translation="MIMKPIKMVDLQTQYLKIKTEIDSAIQDVIDTTAFINGPAINAL
                     KNILKKYLNVKHVIPCANGTDALQLALMSLDLKPGDEVITPTFTFISTVEVVALLGLK
                     PVFVDVDPDTFNINVDDIKNAITDKTKAIIPVHLFGQCADMEVIMNIARENNIYVIED
                     NAQAIGADFNFADGNTMKAGTIGDIGCTSFFPSKNLGCYGDGGAIFTNSDELADKLSY
                     IANHGMKIKYHHEYIGVNSRLDTLQAAILKVKLQYLDDYIMARQKAANYYDNAFANES
                     NLKIPKRFAKSTHVFHQYTLQTNNFSRDELIKFLAERQIPSMIYYPVPIHMQKAYQKY
                     VKTNDYFPISEYLAQNVISLPMHTELDEEQLEYITKSVLEFINK"
     gene            45388..46578
                     /gene="kbl"
                     /locus_tag="PKI65_00550"
     CDS             45388..46578
                     /gene="kbl"
                     /locus_tag="PKI65_00550"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOB76965.1"
                     /translation="MYKDFQEYLIKELASIYEAGLAKEERIIVSPQGAVIRLADGKEL
                     LNFCANNYLGLSNHPRLKQAATKALETHGYGMSSVRFICGTTDLHKELERKIAEFFGT
                     EDTILYAACFDANAGLFEPLLTEKDAIISDALNHASIIDGVRLCKAQRFIYANGDMNE
                     LEEQLKKTQNLRFRVIATDGVFSMDGNVAPVDKIIELAKKYNAMVMIDESHSAGVVGE
                     TGRGVTELFNCKGQVEMITGTLGKAFGGAIGGFTTGKKEIIEMLRQRSRPYLFSNSLP
                     PMIVAAGIEMFYMIDETNELQDKLHDNVKYFMQQIQKVGFDVKPTQSAIVAVMLYDAK
                     VASGMAKRLSDEGIYVTGFSYPVVPKDQARIRVQLSAAHEYEHIDKCVNAFEKVGKEM
                     GILK"
     gene            46767..47600
                     /locus_tag="PKI65_00555"
     CDS             46767..47600
                     /locus_tag="PKI65_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOB76966.1"
                     /translation="MYPTKIALVTGASSGIGRALCIQLAKKGYFVAGMSRNMEKLISL
                     QNKIKRFNIEFLPVQGDVRIEYDCKKFIDTVIEKWGHIDILINNAGISMRALFDELNL
                     EVLHELMNTNFWGTVYCTYYALPYLLEAKGSLVGVSSIAGYQALPARSGYSASKSAIH
                     GLLNTIRIENLYTGLHVMIACPGFTSSNIRNTALTADGSPQNETPLEEGKLMSAEMVA
                     KKIIKGIEKRKRQVIMTTEGKLTVFLGKFFPAFVDKLVFKNLLKEKNSPLIKKYQYGK
                     N"
     gene            47587..48030
                     /locus_tag="PKI65_00560"
     CDS             47587..48030
                     /locus_tag="PKI65_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76967.1"
                     /translation="MEKIKKLEIIPLEGSNLFKFGDSQDLILETLGEPEDIELFEDED
                     EDEPNTSIWFYENNISLFFDEVDEDYFILKAIESSYPETYFKGTKIIGMSDNDLKNFL
                     KSIGYDKFDEEIEDWGEKRISIEEDMIDFYIEDDTVISVAWSSEY"
     gene            48272..49486
                     /locus_tag="PKI65_00565"
     CDS             48272..49486
                     /locus_tag="PKI65_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOB76968.1"
                     /translation="MKPIIEIENLTKIYRLGKLHGSYLTFRDAITQVFKINKNRQKRQ
                     YIKALDNVNLCIQKGEKIGIIGKNGAGKSTLLKILSRITYPTEGIAILRGRTASLLEV
                     GTGFHGELTGRENIYLNGAILGMKRHEITEKFDDIVAFSGVEKFIDTPVKHYSSGMQM
                     RLAFAVSAFLEADIMLVDEVLAVGDAEFQKKCLGKMGEVSTKEGRTIIFVSHNLNAIA
                     NLCEKTILFVDGRVEMFDYTEKVINKYINEIAQHETSKYWDKFDNNYVRPISFKILNC
                     ENKEKFKFQNIEDIYFEIVYEKLSDDDTYPNIHLLTSNNQYVLTSIIDDNKLPKGKGI
                     FKVYAKLEKELLNQGLYHVGLAFTNFKKNRVDFFENYCISFEIVDIPENRKYPYFAPL
                     SGIIRRELTWIV"
     gene            49522..50031
                     /locus_tag="PKI65_00570"
     CDS             49522..50031
                     /locus_tag="PKI65_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOB76969.1"
                     /translation="MDIFEALLTRRSIREYTGEAIEKEKIIEALKYAMYAPSAHNSRS
                     WRFILTSKKEHLIKISEIHPYAKMLPNASWAVLFCNDLDAEPTEGYGALNVAACIENF
                     LLAIHGMGYGAVWIGIYPRKQRIQAIKKFFELPDNLLPIAICSVGVPAIKPTTVDRYE
                     PEKILKIYE"
     gene            complement(50116..50673)
                     /locus_tag="PKI65_00575"
     CDS             complement(50116..50673)
                     /locus_tag="PKI65_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76970.1"
                     /translation="MDIIPILAGISIGFFLMATSYRHALIFKSNFQIIYVNLIFAILS
                     ILMYFLGFQLGLATRYGMPFNPSIISIILFLFLSIRMYLSIAKGKLSELIVDIGKFKN
                     VIALSFSMSFESFIAALGVSYIFTNFTRSFIYTIVLILLFLFVGTYAGKQTNRLSSVK
                     IFFILSSIFYFINGLICIFVTIFTQ"
     gene            complement(50879..51934)
                     /locus_tag="PKI65_00580"
     CDS             complement(50879..51934)
                     /locus_tag="PKI65_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB76971.1"
                     /translation="MKKLTIILLLTLMLSVCMLMGQITITYDDMPPIPFSIIQNSTSV
                     IGGIDISNNGANQTWNLSNLTPNDYLSIDLVDLSTLSFADQYPNGNYAISLENTYYTI
                     ISHTTNNLAIVGLTIPIEEFNITAYLKYLYPELVYTFPYTMGTVVESYPKIKTKLRID
                     TTINNIYIDSVAVELKKYVKREAVGWGTTKLPNGDFQTLKTLVQSKDTTTLLAKLYGL
                     SWMPVYGDTLESNSIEFQMKNLLYPLATITLNNNLQPTQISWVNVDPSLNITQAISSH
                     VKYFPNPVQDFITFEADKPIAIIEIFSLDGKLILKQDAPPSTIFQIDLSELCSGNYVF
                     RLYSNNEIIKTGKLTKE"
     gene            52039..53874
                     /locus_tag="PKI65_00585"
     CDS             52039..53874
                     /locus_tag="PKI65_00585"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOB76972.1"
                     /translation="MKKLLSLIIILVCSTIVYGQTLIVDENFDSYIAGNKFVQQAGSP
                     WTTWSNQPGSSEDPIVSDAQYVSSPNSLYIQNNNDLVLKLNAYTTGRYSISFKIFVST
                     GSLGYFNILNSFAGSSSVWAMQAYFKNNGYLVVDAGGASIDSIAYTQNDWHDVLIVVD
                     LDNDIATIEFDEQFFHLWKFSSGTFGDGNMKKLDAIDFFGWSEGGLNANFYIDDMKIQ
                     EMPAPEMPINLTATLQNQFDIHLNWTAPTTIPNCYLLVRNDRLAAIINASETEYIEAH
                     VYPQTVNYKLFSYVTSQGASAPALTQITIPGGVDRNFVLFEIATATNCPYCPSAARGI
                     NDLITNNANVAVINYHIGDSYATTDCNTRLSFYTSQVGTPTTWVDGVLNLSGGATGTT
                     SLYSAYNPMYQSRISIPSVYINYININKVNDNQYEAKVSVKEHTNFFTSGLKLHVALV
                     ESHIPQTWYTLSELNYVCRKMYPSGNGTNLDFSQSDSLSFTFNINVDAGIVVSNCELI
                     AFVQHQPTKEVIQTIKAPLDPSSIIQLNTNNINIYPSPASDYIYVSEAQGMHMQFINL
                     NGQIVKDQLISSSNFPIYVNDLPSGVYIVTFAENPQIKQKVIIMH"
     gene            complement(53928..55160)
                     /locus_tag="PKI65_00590"
     CDS             complement(53928..55160)
                     /locus_tag="PKI65_00590"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOB76973.1"
                     /translation="MDNIIYDIPDILDDIRLGKLIIVVDDEDRENEGDFILAAEKITP
                     EIVNFMSKHGRGLICTPISQEVANKFKLPLMVEHNTATHNTAFTVSIDLLGYGNTTGI
                     SAADRARTIYWLTRDNAKPEDFGRPGHIFPLIAKPGGVLQRAGHTEASVDLCRLAGLK
                     PIAVLVEILNDDGTMARLPQLIEIAKKFDIKITSVKRLIQYRLKHESLIKQVEEVFLP
                     TEYGNFQLKAFLQITNNLPHLALVKGKWEADESILVRVHSSCVTGDIFGSYKCDCGPQ
                     LHRAMQMIENEGKGVIVYMNQEGRGIGLLNKLKAYHLQEEGLDTVDANLALGFKDDER
                     DYGIGAQILRALGAKKIRLITNNPIKKIALEGYGLEIVETIPLEIEPNPYNRFYLQTK
                     ATKMGHNISILDNKIENK"
     gene            55306..55722
                     /locus_tag="PKI65_00595"
     CDS             55306..55722
                     /locus_tag="PKI65_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOB76974.1"
                     /translation="MKKYLFALFILLAFSINNYAQNIKATDTINNDNEPIFALVQEPA
                     KYPGGDEALLKFIHKNIKYPKQAIKNNISGTVYVQFITEKDGSISDVKVIRGIGGGCD
                     EEAVRIVNKMPKWIPAKQKGVPVRYLNVIPITFKIK"
     gene            55894..57900
                     /gene="uvrB"
                     /locus_tag="PKI65_00600"
     CDS             55894..57900
                     /gene="uvrB"
                     /locus_tag="PKI65_00600"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOB76975.1"
                     /translation="MKFKLVSEYKPTGDQPEAIEQLINGILNGEQYQILMGATGTGKT
                     FTMANVIAQLQRPTLVISHNKTLAAQLYQEFKRFFPDNLVEYFVSYYDYYQPEAYLPS
                     TNTYIAKDLSINEEIEKLRLATTSALMSGRRDIIVVASVSCIYGLGNPNDFLANSFQI
                     RLGQHISAKQLIEKLVNAMYHREDYELNIGTFRLKGDTLEIMQGYGEIIYRILFFNDQ
                     IEQIESYNGIDYTLLSIPEEVTIYPASIFMTTKEKLFKAIDRIVLDLGEQLKFLRNQN
                     KFEEAQRLEDRVVNDIEMLKELGYCSGIENYSRYFDDRKPGERPFCLIDYFPKDYLMF
                     IDESHVTIPQIRGMYGGDRTRKEILVEYGFRLPSALDNRPLTFDEFESLINQVIYVSA
                     TPGDFEFLKTEGAYAEQIIRPTGIPDPEVIVKPTKNQIDDLLAEIKKVREHDGRILVT
                     TLTKKMAEELSTFLTDLNIPTRYIHSDVETLDRVEILKSLRTGDFDVLVGVNLLREGL
                     DLPEVELVVILDADKEGFLRNYRSLIQTAGRAARNVNSKVILYADKITDSIHKMITET
                     SRRREKQIKYNEEHNITPTSVIKEISYSLISDDNKNYIYVNTNDNMSIAEKQAVYYTE
                     REIKENIKKLEKEMKQASKELDFIKAAYLRDEIIKLKNLLRKNK"
     gene            complement(57966..59999)
                     /locus_tag="PKI65_00605"
     CDS             complement(57966..59999)
                     /locus_tag="PKI65_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HOB76976.1"
                     /translation="MIFLYPGRLWFLLLLLIPLIIHLFDFRKTIKAYFPHIKILEALA
                     EKTKREQTLKRWLLFAIRFLAFGFLILAFCMPVRKSEITETTSGDKLLIAIDNSLSMR
                     SFNGTFTLLQQAKDLAKEAVANQSASTIISIIPTSTSVKPDYHLKNECTHFIDSITYR
                     PSQILNYKTFLNTLIESKAKSIIIFSDFQKTDFPLQFFQYLDSMESMVYLMPIQRSSI
                     TNISIDSIYLGAPVIVKNTQGTLYIKLKNWNNDIEEGVKIDLYINNKSVGSAISNLPP
                     NTQTTQEISFPVIDGSYITGKVSIVDDGYDFDNSMFFAINIPSVIQVYHLASDEIRSF
                     ISFIFKGDSLFKYNKINIDELPLTANHQSTLYILESLKNISPDIFNHLLTQVERGNAL
                     LIIPPKENANYLNSLIANLGISFSDVMDKQQLRIENIAYYSPFFKGMFTSTPRDFDFP
                     LVFKHYRLPISKGTNLLSLTNNDPFLKQINHGKGQIYIFASPLQSEYTTLIQHQLFVP
                     IILQIAFSSAQALDLYYYINQETGIPVPFTLPARGDILESTDEAIFLLDLVKNEKYIP
                     FISKAKQPILFLKNIISRADIYYLYYKDNKLPLAFNYPRHESDPRYWSKNELDSLIKN
                     SNNIVIGKYPVAFGPSNLIIHTKKLPIWLIFVISSIILLISEIILLRLWKDRV"
     gene            complement(60060..61160)
                     /locus_tag="PKI65_00610"
     CDS             complement(60060..61160)
                     /locus_tag="PKI65_00610"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOB76977.1"
                     /translation="MKELIIHKWWISNDMQAFTTIDDKLIKVIEPGKYNLHDGPDFLG
                     TTIEVDGIKLIGNTEIHICEEDYRRHRHIENPHFTNVILHVFCFPASKPILEIPFRIQ
                     VSPEIILNDNNNKQNYQFSYDDLLLEGICRLRQIGNQLINDFASNDLIFTLYISLFRA
                     MGLKDNAIPMTMLANKISYKIFYDRYNLNEIKVAYLFASGLYNEINPSIEMISKKYQL
                     LPLSKDIWMHKGIRPNAYPIIRIPKAVECFYYLVNEADFFHKLIINGCDFWFKKLNKI
                     LTIENKKIILANCILPLRIWYAERYGLMNLMEHIINEGSKYDAENNKLIEKACQDLNL
                     DIKSLNLIQAQGVLSLIKKGMIRSDFGKNMIT"
     gene            61196..62062
                     /locus_tag="PKI65_00615"
     CDS             61196..62062
                     /locus_tag="PKI65_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOB76978.1"
                     /translation="MKSLKISPSYLELHLMVLLFGITGVLGKLISLSALSLVFYRMLI
                     AALSLLFWLKRKNLKLNLSKKVFCISIFTGIIMALHWYLFFYAIKISTVSLVLGLLGT
                     GTLFTSFLEPLILKKKFSYLDFIFAIPILLGVLLIYWGEPVQWKGLVIAIISYLLFSL
                     FSVINKKIGIDYDAVVISFWEFLSGSIFMLILTLANEKLTLPNITDSIYLLILGVACT
                     AYAFSALIRLMQKYSAYLVVMHVNLEPVYAILLSLLIFGESEQLSLLFYLGASLIILT
                     LFLYQLKGKQEI"
BASE COUNT        22318 a         9205 c         8881 g        21890 t
ORIGIN      
        1 tcttatgtgg taagctaaaa tttgattacc cacactatcc gttatagaac caaaactttt
       61 tattatattg cattgaacaa ttctaacata taaaacaaat taataatatt attttgtaat
      121 attttaattt gttatccgtg aaatataatt ttgctgatta tttcacagtt tatacaaata
      181 tcaacaaatc aatttataac tattcgaatg tttatcaaag ttgttaaggt tttattaatt
      241 tttatcattt taattattaa cattaataca aaattttatt catttataat agtgatagtt
      301 actaatctac aatgcaacaa agcaccgaaa tcggacttgc aactttgttt gggtagttga
      361 atagtaaaac gactttgtga tatgccatta ccatttaata aatagaaaat attcattgct
      421 cttttttcag ctaatttaat atcatcagat tcgttcgttt cttgatagcc tttaatcaat
      481 atttttaatg aaggattttc gcgtaaaact tcaactacat aagaaaaaaa acgataaata
      541 gtatcattta atttatatga attaggttcg aaatatataa aaaaagtttt agatccgccc
      601 tcatcaggta ttaattgcac taatgtatct tccctgttgg gagttttgat tatatctttg
      661 gcagagatat ctatctcatc tatatcaaat aactgactat caagagtagt atttaatgat
      721 aaaaaataca atgtagaatc tatacctatt tcacataaca aaacatcatc cacataataa
      781 taagcctctc gtttgccaaa tgaaaatatc gattttcttt taaatttatc tctattgtta
      841 agatcgaaat taccgatagt gagaaaaagt tcattcccca tagcagtaaa tgtaccactg
      901 atttttaacc attcattttc aggatcatca ctttttagcc ctttttcgtc tcttacttct
      961 atctgaggtc tatatgtttc taatactagg ggcgataggt aatttggtgc attaggataa
     1021 aagcttgcac cgaaagattt agcatagcaa ttgctatatt tggatggggt aatatacacc
     1081 tcgaagtaat atattttacc tttttctaaa gttttgttta gttttacttg caaaaattca
     1141 ttataatttt tgggccaaaa acgtaaacct gcataagcat tgcctgtgcg agcaggccta
     1201 aggatataat tcttgtcaaa ctttatttta tttacgtctt gtattttttt acttgtacta
     1261 taaacgaaat agtctgctgt atttaatttt ttccatggtt tcaatgtagt tttaccattg
     1321 ccttcgaagc ttgcatttgg caccaagttt tcgcattctt caggataaaa atcttgagct
     1381 aataaaaaat ttatatttaa tattagaata attacaaata ttaatctcat tttattctat
     1441 tggagaaaat atttttttaa aataaggtat atgccataag gcatggaata taccaatcaa
     1501 taccattact atacctatct gtacatgcca atataaaaaa tcgttatata gaaccttgtg
     1561 aggaaaatcg taatttattt gtataattaa taaaatacct aacaggcaag aaactaaaaa
     1621 actaatcaat aataataaat tccatatacg cttgtgcaat cttttgctta ttactttatt
     1681 ctttgataat atccaagata ataaatataa aattatcgtt gtagccccga tctctattaa
     1741 attatatgaa cgtttcttaa tatttaacct caaaactggt aaaacattaa aatgattttg
     1801 ataatttact aaatattgtt gtatagtttt atggataaaa ttattattta ttccattatc
     1861 atttggcaca tcatccactg atgataatgt catattttgt gagattgtat cgatattttt
     1921 attttctaat ttgataccaa aatcacaata accatcttga tcatcatcaa tgtatttgcc
     1981 acaattgccc gtgcaattta cattatcaca tatatgctta gtagtttgtg cattaatatt
     2041 gacacaaaaa actaaaatat atgcagtaaa aattataatg gctatacgtt tcattattat
     2101 tttttacaaa gttatttaaa atttatctaa ttattactca atctattaat tttttttata
     2161 catttatcta tctgtatgca aattgtaatt tttgtatata tttgctaaac atttaaagta
     2221 ataataattt aatattatat aaatgttatg gaaaaaattt tagttatacc acatccaata
     2281 gagatatccg agaagcaaat taggttttta aaagatgaat tgagcaaata taatgtcaag
     2341 ctagtttact ataccgatga accctctaat gatgaagaat tgataaatcg aataagtaat
     2401 gctaattatt taattatagc tgtattacca ttaaacgctg atatattgag caaagcaaag
     2461 aatttagaat atatttctat tacttttact ggttacgatc atgtagatat aaattattgc
     2521 aaagctaata atatcaaagt aagtaatgta gctggatatt ctactacagc tgtagctgaa
     2581 ctgactattg ctatggctat aatgctctat cgtcaattaa tacctcaata tttatctttt
     2641 aaattatctg gtgataaaat aaataaacca ggtagagaaa tatataataa aacatgggga
     2701 gtgataggct acggtaacat aggtaaaaaa gtaacagaat atgctaaaag tttaggtgct
     2761 aatataatag tgcatactcg ctctctgcac cccgaagctt ctaatataga tttcgtttct
     2821 aaacaacatt taatagccaa ttctgatatt ataagcatac atatgccact aaataacgaa
     2881 accaaacatt ttataagttt tagagagttt gatcagatga aagataatac aattttaata
     2941 aatacagcta ggggattaat agtaaatcaa gaagctttaa tagatgcttt gataaacaaa
     3001 aaaatagcag gtgcagcatc tgacgtattt gagatagaac cccctctcga tccacaatat
     3061 cctttattac atattgataa ctttttagct ctgccacaca ttggctatta taccgaagaa
     3121 gcattagaaa aaagagccga cataggaatt gataattttc ttaattattt gaatggcaaa
     3181 ataactaatg aagtttgtta agtgaccatt aaacaaataa aactggagtg gctaagagtg
     3241 agaaactgaa aaaaacattg agaaataatt tttgaatgtt taatttacaa tttagaaatt
     3301 gttgtttagg acttttttgc aattaatatt acattcttga ttttttattc gcaaatattc
     3361 caaaaaaata aataattttg acttttaatt tttaataaaa ttttttttga ctatttttga
     3421 atttataagg taaaaaagtt taatttatta aaaaagttaa aaaaaaaatt aacaatatgt
     3481 taaaagcatt tgcttattga gcaaattgag gtaattgata gatttgttta tataaaaact
     3541 taagttattt gctaaagctt atttcaataa tctcacagat tcattatatc atttaccata
     3601 tttgttcata tagtattata aaacattgtt caaatagttt attaattata aaatatgtta
     3661 acagaagata ataaaaataa taaagagtca gctcttaaaa taaatgaagg aatcgaacaa
     3721 ccatcccaag taaatcaaga agtaataaat aaatttttgt caaaaacaaa aacaccttct
     3781 actcaagaat taatagaagg aataaaaaat ggtgatagag ttgctctgtc gagagctatt
     3841 acattaatag aaagtctaaa tccgagacat tattgcgaag ctcaaaaaat aattgaagct
     3901 tgcttgcctc tcagtgggaa tagtataaga ataggtgtaa ctggtatacc aggagcgggc
     3961 aaaagtactt ttatagaatc ttttggtact atgcttacag agttagatta taaagtagct
     4021 gttttagcga tagatccttc tagtcaatta tctaaaggaa gcatattggg cgacaaagta
     4081 agaatggaac gactagcaac aaatccaaaa gcatttattc gtccttcacc ttcggcaggt
     4141 actctggggg gagtatctag aaaaactaaa gaaactatga ttttatgcga agcggctggt
     4201 tttgatatag tattgataga aactgtaggg gtcggacaaa atgaagtagc agtgagaaat
     4261 atggtagatt tctttctctt acttctagta gctggcgcag gcgatgagtt gcaaggtatg
     4321 aaacgaggta tcatcgaaat ggctgatgct atttttatta ataaagctga tggagacaat
     4381 atcaataaag ctctagcctc tgctcaagaa tataaactag ctctccatct tttgccacca
     4441 caccctgcag gatggacgcc tatagtagat gtttgtagtg ctatcacagg atacaatctc
     4501 gacaaagttt gggaaacaat tcaaaaatat ataaatcttg taaaacaaaa tgcatatttc
     4561 gaaaaaaata gacaaaatca agaagctcaa cgaatatacg attatttgat agaagagcta
     4621 aacatgtttt tttggaacaa cgaaaaatta caaaatgaat ataataaatt aaaagatctg
     4681 gtactaaagc aaaaaatcac tgcatatcaa gcagctatta aattgaaaaa attatttatc
     4741 aaattaatat ctaaacgata attaattttt tttttagctg cctcctaatt ctctaaaaag
     4801 atttttaata tttagcattt gtatcatgag ctccattgga tgatagtcat agatttcatt
     4861 agtagtgcaa cattcatcca caaaaaacca actatacttt attgtattga aatttggcaa
     4921 ttcaatgtat ttattattta tgatttttaa tttttgataa tatacactat ccaaataggt
     4981 agtaggctta tatgcttgat ttttgctttc cattattatt tttttattat atttatgttg
     5041 caattctatg atttctttgt ttaccctttc attattttgt ttagcagata atattttttc
     5101 tatcgaccaa attggttcaa attttagtaa aataaatacc tgtaccttat cagttataac
     5161 tgattctagg ctatcagact cgatattttt atttttacaa tataaagaat cttcacaata
     5221 aatttttaaa tatggcttat ctaattcaaa atgccaattc gagggtaaaa cagataaaat
     5281 ttttgatagt gtaggatcat ctttagccca tgctaaaaca agtgaatcag aagaattatt
     5341 aacaccaaac aaaaaaatat attgaaaaaa tataaataaa atactcaaat aattcctttt
     5401 catcttaaac ttttagaata atacatacta aaatcgtgcc aacattagat aaaaatttga
     5461 aaatattata tttttttaca agtaatataa ttgtgatttt attatttgta attgattaca
     5521 aataattaca aacgaatata ttcgtaagta attgattatt atccgactac agcgagagct
     5581 tgatattaat tttgccaaac aaacatttca aaaaagagtg aattatgaaa acacaagaaa
     5641 aaaacaacga aaaaatgtta aacgaagtag aaaatggtca agtagaagaa tttagcgaag
     5701 atttatgctt agacgaaaat gcttttgcag agaaattagc tggtattatc tctgaacaac
     5761 gtagcgtaaa tatgctatta ttaaaaggtt tcatagcttc tgatgtacaa gtattcaatt
     5821 atggtacctg ggagaaatat agctttacgc ttggagtatc tgaacgcaaa tggaatggtg
     5881 caaactttga atactataat caatatttac caataacact aaatggtgta ttagctgata
     5941 tagtaggcaa gcaatataaa aaaggaaatg aaataatagt attggctact atacaccgta
     6001 gagtaaaaga aattgatgga aagcgtagag ctttttatga catcaaagct aatgatctct
     6061 atgctgtaaa taagtcacaa tcagctaaag tagcttaaaa aagacaaaaa aaataaaaaa
     6121 agcggagtag atgctccgct tttttttatt gattaaatga aattatccaa aaaagttttc
     6181 tcaaaatttt tataacaagg tatagaatat taattatcta taaaaaatta aaaaaaaatc
     6241 tcaaaaataa actaacaaaa cgatgtttat tagttaaaat ttttaaataa ataaataaaa
     6301 aaaaattatt tattttattc caattcaaaa atttgatata aatttgtcaa aaatataaat
     6361 ggcaacaaca tcagatataa gtaacggaat ttgtatagaa tttaatggtg aactatacac
     6421 agtggtagaa tttcaacatg taaaacctgg taaaggtgct gcatttgtta gaacaaagct
     6481 aaagcatata cccagtggca aaatcataca aaatactttt gcatcaggtg ctaaaataaa
     6541 tatacaacgt atcgagagac gtccttatca atttttatat aaggaaaatg aatattatca
     6601 ttttatgcat acagagactt ttgagcaaat aatgatttct gaatcattga taaatgctcc
     6661 tcaatttctt aaagaaggtc aagaggttga aatcatattt catgcaaata gtgagacacc
     6721 acttttatgc gaattaccta cctacgttgt attagaagtt acttatagtg aaccaggagt
     6781 acggggagat acagctacta atgctatgaa attggcaatt ttagaaactg gggtagaaat
     6841 aaaagtacct ttatttataa atgtaggcga caaaataaaa gtaaacacgc aaacaggaga
     6901 atatggtgaa agagttaata attaatatta aaaattttaa aaaaatactt gaaaaatgac
     6961 actagataga tcatacagta ttcaagaaat tatttcaata attgataatc cacttgcaaa
     7021 agtaatagga gatacttcta taaaagcaac aggtataaat gagatacata gagtgaatcc
     7081 gggagatatc accttcgtcg ataatgaaaa atattttaat cgcgttttat cttgtgatgc
     7141 ttctatcatt atattaaata aagaaataga agctccagat ggaaaagttt taatatactc
     7201 agacgatcca tttagagatt atgtaaaatt ggttaaattt tttgctcctt ttataccaca
     7261 agatatggcc attcatccaa gtgcagaaat aggcaaaggc actataattc aacctcttgt
     7321 atttattgga ccaaatgtta agattggtga aaattgtatc attcacccta acgtaagcat
     7381 tcatgcaaat agcattatag gcaataatgt tataattaat agtggttctg taataggcgg
     7441 agaagctttt tactttaaac gtagacctga ctatttagac aaattagaag gttgtggtag
     7501 agtagtaata gaggataacg tggaaatagg atcttgctgt actatcgata gaggagtatc
     7561 tgctgatact ataataggga aaggaactaa gttggacaat ttgatacaag taggacatga
     7621 taccataata ggaaaaaatt gtttaatagc agctcaagct ggcatagctg gtgtagtaaa
     7681 catagaagat aatgtgatac tttggggaca agtaggtgta caaaaagatc ttactattgg
     7741 taaaggggct gtagttttag gacaatcggg gatagctaaa tcaattgagc ccggaaaagt
     7801 gtattttggt agtccagcta gagaagcatc agataaaatg agagaaattg cattattaaa
     7861 aaaattacct gaactctttg aaatagtaaa taacttaatg agaattcatg gtatcgactc
     7921 agatctaaaa ataaataata aaaatcttaa taattttgaa taattttttt ttaattatta
     7981 tgattatgaa gtaagaaaat aatgacatcg atgctaattc atttaccata atggtaggat
     8041 gcaaaagtct acaatatgcg acatatcata ccaggtacta gctcggcttc tatagtcaaa
     8101 agtaattcac cgttttcaga aattttataa actgatccat tgtgcatata atcttttaca
     8161 tcactcaccc agaagcagtt atcatcacca tcccacatta gataatacaa actagaacta
     8221 ctagaagcag catataaagt attgatagac aatatgttat gatcatcaat agtagcagat
     8281 tgtatagcat tattattcag aaaatacatt ttgccattcc taataatcag ataagatgga
     8341 taatcattat tattaggaaa attccaatag tgaacgattt gatgattatc taaagaaatt
     8401 ttagaaattt tagcattttc ttcgtgcata tatccacctg tagacagtac ccataagtaa
     8461 ttatctttgt acacgatagt ttgaggttct ataccgactt cgatgctatc aattaattga
     8521 ttctgaacta gatctataac cattacaaat ttattagctt tgccactata atagttggac
     8581 caagctaatc cataaatttt actatttacc tcaattatct gctctattgg cctatcacaa
     8641 taaattttag aaatactatt gtctattgag ttaatcacat ataaataaga ttcagtaagg
     8701 ctagatacat aaatatgtcg taaatctcta aaaactattt ctctaggtga tggcacatca
     8761 tactcactca aaagtaaaaa attatgcaga tttacaatat aaactttatt agaattattg
     8821 actgtgataa tggctacatc atcagataca gctagtctgt atggtagatc accgagcagt
     8881 ttattattaa tattataaaa aatctgatga ctaactttat tagtagaaaa atcataataa
     8941 gatatctcac cattaccttg accaaaaagc ccttcattga tgataagaac tccatcagtt
     9001 ataaagctaa catcattatc aggatttact ggtgtctcat ttggctcatc attattacaa
     9061 cttattaata tgagagcgaa aactaaaaaa ataattaaat ttaatttttt cataatttta
     9121 ttttttatat ggtttctaaa aattataatt ctatttgcaa ggaaaaaata taatatcttc
     9181 gaggcatagg ataccaagct actgtacagt aattagcatt agttaaattt tttacccaaa
     9241 tgctataatg catagatact tttttgatat tatgctcgta agataatcca agattttgaa
     9301 ctgcatacca gggtaaatat ctatcattat caaaagtact aaatcgttta gaattgtacg
     9361 aatattcata atttattttc caatgatttt ttattgataa attaataagt tcattaaaaa
     9421 tataattagg tacataaata agctctttgt gagtttctct atcctcagca tattgaacca
     9481 aaaaattaga tgtaaagcgt aaaataaaat tattaaacgt aaaatctagt ttccaattag
     9541 cttgtattcc attttgaata gctttattta tattaattgg ggtccagata gcttgatttt
     9601 cagagggttt ccattgaatc aaattattgg taaaataata aaaaggattt atttcgcaaa
     9661 tagaatagaa aaatccttta ttaaataacc atttcacttt aatatcaaaa ttattacttg
     9721 cttctggtat cagatcagga ttaccacctg gcacccaata taaatcattg aatgagggca
     9781 atctctccag atggctgaga gcacccgcta caataaaatt gtgattacca tatttccact
     9841 ttttgctaac tcctattccg ccctgtggat aatatttttt aaaattttta atttgcaatt
     9901 gctcgcttac ccaccattgc cagccattat tttcgtaatt ataaattaac gattgatctg
     9961 taatatatct atcagctatt ttctgataag cagagctatt caaatattga taagaaaatc
    10021 taattcttaa caacaactga tgatctccaa aagtaaaatt actctgatga tatatttgtg
    10081 gagctaaact attagaaaca gtttctgctg gcggtaaatt agaattatat tcatattgct
    10141 cactatacca accaaaggaa gaataatgct gccaatgaaa gctcgatgac caattatatc
    10201 tcagataagc taattgaata ttttcatttt gcgtagcatt attatctaat tgtgcactta
    10261 atatagaagc tggcacttca ttttggtgat actgaatatt aaaacccatc tgccatttat
    10321 ttaatttaat atcaaagctg agctgttgtc ccaaatgctc attatctctt ttcacttcta
    10381 atttattatt atgcattgta tttataaatt cataataatt tttatcaaaa cttccattgt
    10441 attgaactct aaaggcatta ccatttttta actgttgtga aatagaaaat tctatatcat
    10501 tattattaat agtagcaaat gaatatccaa tataataatg attcttgcca gcaaaaactg
    10561 tttcagcttc tatgatggaa cctaaggcac ctggaaaacc taaggaacta aaagcacctt
    10621 cataattatt aatacgaaaa ttattagaaa aaaaagataa ccataaactc tgccctaaag
    10681 aatatgacaa taaaggaata tcattccaaa taagtaaagt atgaccagca ccaaaaccac
    10741 gaaaagaaat agaagcattc tgcacaccat attcctttag atatacaggt gtttgcattt
    10801 ttattatttg caaccattgt ctttctgcaa taggtatgaa atgcatagaa tcgctaatag
    10861 agaaactatt aataaaagta gaaagtctac ccattttttg tgtagaatca atgatttcaa
    10921 tcttttgcaa ataaatggta tcctgacaac aagaataatc agaattgaaa ataatataaa
    10981 taaaacaaat aataataata atcgtatgct tataaaaatt aatcataaaa atgattttaa
    11041 taattttcta ctccttttga ccccgaaaag aatagtccta aaaaaggata aaaagtttta
    11101 gcaggtcttc tggcttgctc acctctatca ccttcccatc ttataaaaag atagtggtta
    11161 atagtattaa gatagaggta aaaaagagct aacagctgcg ggcacagctc gggattcaca
    11221 cccgattccc tattagtttg tttataatga atttttatta aacaaacact aaaactaatt
    11281 gcaaaaatat ataataaatt tagaagaaaa aaattcaaat aaatattgtg aatggatcat
    11341 tttaattatt tcagctttgt tctaaaatta ttaattaact tattgttggt attttttttg
    11401 ctctattcaa taaatgagtt ttgattagtg aaataatcat aatgttattt ctgtgttata
    11461 attctgaata tagctaaaat aaataagaaa cattggtaat tttaattgaa ttaaaaaagc
    11521 ctattgagta tatttaaaag gaatcgaaaa aagtccagaa acaaatcatt gttttacttg
    11581 aagtagttta tctatatttc gtattcacca gccgccaata ctcatcccac gcccatctgt
    11641 ccgaggatgc gaggtttaaa agccgaccaa tgtctgattc tatctcactt gtcaagtatt
    11701 tatgtgtgat tacaaaccac atcagatagc tctgcaagta tttggtagcc actcctctaa
    11761 atcgttgcaa ccaaaccttg aaatcattta tatgcttatc cacaacctgt acactgtata
    11821 caccgcgtct aatctgcgtt ttgtttacaa caactttttt gcttttggtc cctttcacag
    11881 cttttttgaa ttccttcttt tgcttcacac atattaagtt gctatctgtc actctatgcg
    11941 atagctcttc tcttatctga ctattggtta cttttttgcg tcctacgtgt ttgaaaagta
    12001 aattgccttc cctgtctgtc attactagca cagctacttt gggatgtttc tttttctttg
    12061 ctttttcgta ttcttttcgg cttctgtatt tgcgaccttt ttctgaatat ttcattagca
    12121 gctcatcagc ttctacaatg cctgagaagc tgataccacc ctgtaatgat ttgatcgctg
    12181 ctagtatctt gtgtcgccaa ttgaaactcg ttctcagact tatgcctact tccgatgcgc
    12241 aagctcttag acttttgcct tctaacatgc attttaggta atcgagcatt ttatcagctt
    12301 ttcgcatgcg atatacgaag gttctagctg tttcgcggta ttgtcggccg caatctttgc
    12361 atttgtacac ttgtttgccg tcgcgtactc cagcttttat tacattttcg ctttcgcatc
    12421 taggacagat gggggctgtg ctttgtattt ttagcttctg ataatctaca gccgtgtagt
    12481 tgttttttaa taaaaattga tatagattat ctagaaatct acgtttctgc tctttgggta
    12541 gagcttgata atctaataaa ttaaaattat ccataattat ttttttgtaa aattaaataa
    12601 aaattattta atggtagtat aaaatacgta aagtaaaaca aagaatattg ttcctctaac
    12661 ccccatacaa agcctaatat ttccaaaaaa aattaaatta aatggtcaaa gcaaatagtt
    12721 ttttaaaaaa aattatcatt attttttatt aattttactt ctgatttttt tttaattaaa
    12781 cagtattttt ttttaaaaaa ctatttcatg gcaaaaaaac tcacacctct catgaagcaa
    12841 tattgggaaa taaaaagcca atatccagat acaattcttt tatttagaat aggagatttt
    12901 tatgaaactt ttggtgaaga tgcgcgtata gcatctcaag tactaggaat agttcttacc
    12961 aaacgtcata gtggtgcagc agcagatgaa gatttagctg gttttccaca tcatagttta
    13021 gatacatatc tacctagact tgtacgcgcc ggatatagag tagctatatg tgaacaatta
    13081 gaagatccaa aacttgccaa aactattgta aaaaggggtg ttactgagct tgtaactcct
    13141 ggtgtagttt ttactgataa tgttttaatg caaaaagaaa acaatttttt agcggcttac
    13201 tattatgaaa aagatctaca cggtatagct ctattagatg tatccactgg tgattttttt
    13261 gccactcaag gcgataaaga ctttattaaa cgtattttag aatcacatca tcctaccgaa
    13321 gttttattat caaaagaaca aaaagaatat ttcttagatg atttgggcga ttaccctgtg
    13381 tttacttttg actcttggat ctttcaatat gattttgcac gtgataagct gatagaacaa
    13441 ttcgaaactg aaaacttaaa aggctttggg ctcgaagata tgccattagc tcagatttgt
    13501 gcaggtgtat ctctatatta tgtgagtagc actcaacaga gacaattatc taatattact
    13561 tctattagtc gtattgataa taatgattat atgtggttag ataattttac aatcaaaaat
    13621 ttagaattat tggaacctct acataatgaa ggtatttctt ttcgcaaaat tatagatcac
    13681 tgtgttacac ctatgggttc tagattgctc tcgagatgga ttattatgcc cattattgat
    13741 ataaatatta tcaattggag attaaatatt gtcgatttct ttgtaaataa taatgagaaa
    13801 ttacaactta taagagaaaa tcttagctct attggtgata tcgaacgaat ggttggtaga
    13861 ttagcaagtc aaaagataaa tcctaaagag ttattacaat tagctaatgc tatcgatgta
    13921 gcatttaata ttcaaaaaat attagatgat gtgcctgatt tttttaataa taaaaaaaag
    13981 atagatcaca aactaataaa tatcgtaaac aaaattagaa atactattgt agaagaacct
    14041 cctgcttcta tacagaaagg cgattgtatt aaagatggta ttaatgtcga attagacgaa
    14101 ttgagaaata ttaaaaatca tagcaaagag attttaaagg aaatactaca aagagaaatt
    14161 aaaaatactg gtattgcaag tctaaagatt ggctttaata atatttttgg ttattatttc
    14221 gaagttacaa atccgcataa aaataaagta cccgcctctt gggttcgcaa acaaacatta
    14281 acgcaagctg aaagatatac taccgaagaa ttaaaaactt atgaagataa aatcataagt
    14341 gcagaaacca gaattatgca gttagaatta gaagaatatg atatactatt agatgggcta
    14401 ataccttact gcagagactt attatacgct ggacatctaa tagcagagtt ggatgtatta
    14461 actaattttg ctcatatagc aataataaat gattataaaa ggcctgtttt aacggatgat
    14521 ttaatcatag atattgatca aggtagacat ccagtgatag aaaaaatgct aaaagctggt
    14581 gagagatata tacctaatga tttgtattta gatactaata atcaacaaat aatagtattg
    14641 acaggtccta atatggctgg taaaagtgca tttcttcgtc aaacagcgct aatagtaata
    14701 atggcccaaa tgggtagctt cgtacctgcc aataaagcaa ttataggtat tgttgataaa
    14761 ctatttacta gagtaggtgc tagtgacaat atatctttag gtgaatctac atttatgatg
    14821 gaaatgaatg aaactgctag tattttaaat aatttgtccg aacgaagttt gatattacta
    14881 gatgaaatag gacgtggcac tagcactttt gatggtatat ctatagctat ggctatcatc
    14941 gaatatctgc ataatcaccc atattataga cctaaaacta tctttgctac tcattatcat
    15001 gaattaaatg aagtcgctaa ttatttagaa agagtaaaaa attttcattt cagtgttaaa
    15061 gagattgata ataaaataat ttttttgaga aaattagaag aaggtggtac tgaacatagc
    15121 tttggcatac atgtagctcg catggctggt atccctaata ttgtagtaaa tagagctaat
    15181 aacattctaa aacaattaga ggctgacaga gaaaataata aatgggaaga aaaaccatta
    15241 aataaaggca tacagcttag tctgttccaa ttagatgatc cattattaga agaactaaga
    15301 caaatgattc tatcattaga tttagatcaa ttgacaccat tagaagctct aatgcaacta
    15361 aatttaataa aatcaaaact gaaatctagt aaataatagc tattacaaaa tcaataatga
    15421 acaaactaat aataaaatca aatataggtt ttaaatatca ttatacatat caattaataa
    15481 aatgttaatt tattgaactg taatataagg taaaactaag tgataaatac tatcatttac
    15541 tgattttaaa ttttttagtt cctcaataga tttataatta ccatgatttt gtttataatt
    15601 ataaatagaa atggctaaat tataccctat ataaggatgc ttagccattt ctttgatatt
    15661 agcattattg atattaattt ttttaatcaa atatggatta atggttaaat aaggtttcag
    15721 tgtttctatt agagaatcat taaatccata aatcattttt aattgatcat aatcaataaa
    15781 tccacctaat ttatttttta actctaaaat tttttttgcc ctatatttgc caatgccggg
    15841 tattttaatt aaatcaatgc tatctgctgc atttagctct atttgaactt ttgctttatc
    15901 cgttttttta gtagttttga cattagaata cgtaatagta tctggcagta atatataagg
    15961 tgctaaaact gcaaatatat catcatctac aaaatataat ttaagtaaat cattttttgt
    16021 cttaaaagta gctcctttgc ttctataatt aataaaagtt tgagtttgtt tatctgtaaa
    16081 tcctaatttt ttaaaatcat tagcactaat agtattcgga tcaaaaggaa atggatttat
    16141 tgaatttact ccccaagttt tataattagt ttgaaaaaga gaatctctac ttgctaatgc
    16201 taaaatccta ttattttgta ataaaattga tatactgtct aaatcatcaa aatttaaggc
    16261 agtaggttta taatgatttt ttaatgtata aggtagtaag gatatgaaaa aaatcaaaat
    16321 tgccaataaa aatattcctc tccattctga gatagataaa tgaaaaaatt tgaaccaaaa
    16381 atttttcata ataataacaa aaaactacct ctcaatcttt ttctggcacc atcactaaat
    16441 agtacattca gataataaac gaatgttttg gtattattat gtattgtttc catatcagat
    16501 ttatcaccac cccaccttgt aaaagggtcg gtagagtaaa agacttcctg tccattcaaa
    16561 ccatatattc tcaattcata agaaacaatg ggataattat aagatataaa aggataaaaa
    16621 taatcattta tcccgtcacc attgggagta aaagcatttg gcataataac tagtggttct
    16681 gaataatcag gattaaaatc tatagtatct gtgattgtct gagcattgca cattactatt
    16741 gaaactgaaa agataattat taatatagaa aaaatttttt tcatttcttg ttttttattt
    16801 ttacaaaatt aattattttt tctcatttca aaataaacta agtacctttt attactttta
    16861 agcttagatc tatgctacca tagtgatgag tcatgtaacc actcgaaata taatctacac
    16921 cagtttctgc ataaactttt atatttttaa gggttatacc accgctgact tctaattctt
    16981 ttttcccatt tacaatagat acagctaatt ttacatcatc tacactgaaa ttatctaaca
    17041 taatacgtaa tactgaatca taatttaata tttcttttac atcatcaata ttgcgtgctt
    17101 cgacttcgat gggtaatttt aaattatttt ttgataaata tatttctact tttttaatag
    17161 cttgtgatat gccaccagcg aagtctatat gattgtcctt tattaatatc atatcatcta
    17221 aaccgaaacg atgattgtac ccacctccta atctcactgc ttctttttcg agtgttctga
    17281 gtaatggagt ggtttttcgt gtatctaata atttagctcg tgtatgtgat atctgatcta
    17341 ccattgctct agtagtagta gctatgccag acatcctttg cataaaatta agtaaaaccc
    17401 tttctataga tagtattgat agttgtttgc cataaagcat gaaaataata tctccagcta
    17461 ctatagcatc accatctgat tttaatagtt ttattgaaat attcggatca tagtaatgga
    17521 ttaaccgctt ggcaaatgat atgcctgcta ttactccatt ttctttggca tacaaagctg
    17581 tattgcccac agcttgtgga tctatgatgg ctatagatgt atgatcgcca tcacctatat
    17641 cctctattaa agcccatttg attatatcat ctatagataa tctattcatt tttttgattt
    17701 ttatattttt ttttaaattt taaagttatc ccaatcagaa gagaacggaa gcttttctct
    17761 tatttttgtt aagtgatcat aataagtatt tacgataaaa atattttcat cttgtttagc
    17821 ctccaaaacc acgctaccag caggatctat acacatagta cccccagagt attcaaatcc
    17881 atttccatct tttcctacac gattaactcc tattacataa gcttgatttt caatagctct
    17941 agctattagt agatgcttcc aatggtaata tcgtttagta ggccagttag atacatatac
    18001 taaaatatcg taatcaaact tattatctgt ctttttgttt ctattccata ctgggaaacg
    18061 tatatcataa catatatagg gacgtatttc ccatccttga taagaaatag taattgtatt
    18121 gctgcctcga gtgaaatata tattctcatc agctaaggaa aataaatgtg ctttatcata
    18181 cttaaaaatt tctccatccg cagaaaacca tataaatcta ttataaagtt tgccattctc
    18241 ttgtatcata aagctaccac aaatagccaa ttgatatttt tttgccatag ataataatct
    18301 atctatcata aaactatcca tagtttgggc tatttctgga tttttagtag cataagcagt
    18361 agtacacact tcgggacaaa ctaatatatc tgcatcaaaa gatttagatt ttttaattac
    18421 agtttctata ttttttagat taccatctat atccatccat aatgtattag cttgatacaa
    18481 gctccatatt agattttttt tcattatttt tttaattttt taaatataat ttaattaatt
    18541 aacattactc aatttatatg agaataattt ataaaaaatt ttgttaaata aataaagttc
    18601 ttagtaataa atgattctca ggctcaatta tctgaaaata ttattatttc ttttcgattt
    18661 ttttagattt cttgccacca tccattatta gccaagctac agaagaagcg atgaaaatag
    18721 atgaataagt acctattatt acacctaaaa tcatagcaaa agtaaagcct tgtatcactt
    18781 cgctaccaag taaaaagatt gccaaaagta ctacaatagt tgtaccagca gtatttatgg
    18841 tacgagatag agtagcatta attgcttcgt tcatattttg ctttaatggt cgtttaggaa
    18901 acagtatatt atattctcgt attctatcaa aaattacaac agtatcatta atagaataac
    18961 caataagtgt aagaattgca gctaagaaat tttgatctat ttcgagatta aaaggtaaaa
    19021 taccatagaa gatgctaaaa aatcctatca ctatgaaagc atcatgaaat aatgctactg
    19081 tagctccaag gctatattgc cattttttga atctgatcat tatatatata aacattgcta
    19141 agagagctat ggctatagag ataaaggcag aacgttgaat atccttagct attgatggac
    19201 ctacattctg tgaactcatt atacctatta ctttatttgg attatcagta gaaaaatctt
    19261 caaaagaaac aggattttta taaaattctt ttaccccatc ataaagtgct ttttctataa
    19321 tagctcctac atttcttgat gtatcctcga gcatatattt agttactata cggacctgat
    19381 tgttattgcc ataaattttt acttctggta cttcatttaa aactttaaaa agagaagatt
    19441 gcacttcggt agcttttacg ggttggtcaa aacgtatcac ataagcgcga ccaccagagt
    19501 aatcgatgcc taattgtaat cccttagttg atagagatac tatagaagcc aaaataacaa
    19561 taccagaaat tataaaaaat gttttgtact tagtgataaa atctatttta gtattagcta
    19621 agaaattttc tgaaaattta tgataaaaag taacattttt gcccttatct agtaataatt
    19681 caataaacaa tctagtaaca aatatcgcag taaataaaga agtcaaaata ccaatcacaa
    19741 gtgtggtagc gaaaccttgt acaggtccca tcccgaatat caataagact acacctgcta
    19801 ttatggtagt aacattgccg tctataatag ctgaataaga gtttttataa ccatctttga
    19861 tagctagccg tagccctttg ccagccctta cttcttcttt tatgcgttca ttaattatta
    19921 cattcgcatc tacggccata ccaagtgtta aaactatacc cgcaatacct ggcagtgtca
    19981 aagtggcacc gatagaagct aacacaccaa aaagtataat caagtttagc aataaagcta
    20041 tactagcacc tataccagca gtaccataga atgttatcat atagattatt actaaaataa
    20101 aagctattaa aaaagcaatc atagtagatt taatagcttc tgagcctaat gatggcccca
    20161 ctacattttc ttctattata cgagctggag cgggtaattt accagcattt aatacagtag
    20221 ctagatcttg agcttcctct atagtaaaat tacctgatat ttgtgatcta ccatttggta
    20281 tttcagattg tacgacagga taagaataca ctagattatc taagacgata gctatacttc
    20341 taccaatatt tttagcagtt agatccttcc atattttagc cccttcagag ttcatttgca
    20401 tatctactat tacattacca tattgatcta cgtcttgccg agcatttact atcacatcac
    20461 cttctaaagg agctttatta gtcaaaggat ttacttttaa ggcaattaat tcatagacat
    20521 tcctcgtatt agcaataggt ttagcactcc ataaaaactt aacatctctg aaaagatctt
    20581 gagtcaaacg aaaatattca ttgatttttg ctgtatcctt agcgtatgca taaccaatta
    20641 gaggtccttc gccataaaat tgttgattac cttcttgtct tatattaata gttagataag
    20701 caaaaagtgg attttctttt ttgtattgtt cgaattcaat atcttcaggg gtttctccga
    20761 gaggtttggt ttctaatgct tttagttgtt gtgctagttt actagtatct tttaaagtat
    20821 cttttttggg tttagattct acaatagtcg ctgtgtcttc gacaaaaaga agagaatctt
    20881 gagatttgag catttgagcc aatcgtttat tagcttgttc gagcctggga gctatctcag
    20941 tcagattcca agtttcccaa aactctaatt tggcagttgt ttgcaaaata cttcttacac
    21001 gattaggatt atctacacca ggcaattcta tcagtatacg actttgcccc actaattttt
    21061 gaatattagg ttgagtgacg ccaaatttat ctatacgttt acgtataaca ataaaagtat
    21121 tgtcaaaagc agattttatc tctcgtttta gataatttat tacatcctga tcagtagaat
    21181 ttacatttaa ataatcacta gtttcgcgag ataaaaaata tgtaacaagt ggctgattag
    21241 gatctatttt ttttatttct tctacaaata aatctacaaa agacacatta ctggctactt
    21301 ttctttcttg agctcttttt agagcttctt cgagtttggg atcattcttt ttagcagcta
    21361 atgcccttat aatagcagca tcatctactt cgattgtaag attcatccct ccttttaaat
    21421 ccagacctaa ttgcagttct ttttctttac attcagcata agtgtacttt tttataagta
    21481 tattatacac ttttacatcc atcatagagt ctagatattg actttgtttt tcgcgagcga
    21541 tagaatctaa taacatcatc attttaatag aatcgcccat agctaatttc tcagcttctt
    21601 tatatgtagc ttcggaataa gcaaattctt tagctttgtt ctctactttt cgcgtacaga
    21661 gtgtaaacga taatgaaaat atcgaagcta ataaaaatag gattacaaaa aaccaaacag
    21721 taccttttaa acgcataatt tatttttttt taattaacac ttttgatttg caaaaataaa
    21781 agaaatattt taattataaa taaaaaattt aaaaaatttt gatatattaa tttttttttg
    21841 taattttgta taacctataa atatcggggc cggattggtt tcgacagcag ggacagtgga
    21901 ttagcttgca tgccgaggat gggatgtgac ctcgtaaatc atcattccaa acaaataaat
    21961 ggcaacgatt attctgttgc attagctgcc taatctagag attagaagct aattgcctcc
    22021 ccagaagctg tgctcaatgg cgtctgggat aaggtatcat aaaaattgag ctcacttaga
    22081 gtgaagctac gacctctaag tgatatattg tagctttgga atttggtggg agcctatccc
    22141 ctaaccaaat tctgaaatat aatagatagg ctaagcatgt agacagctat tttgctgact
    22201 gcttggacga gggttcgact ccctccggct ccatttattt tcaaaataat agtttcttta
    22261 acaaatttta tcatttgtct gagagtgtta caatgcactc aaaatataaa attatatatt
    22321 ttatctgtta cacaatattg aacacaatca tatttgatat taggaattcg cagattacta
    22381 aataataaat tacaaaaatc atagtgtgag atgtattgca atgttctact taatcatata
    22441 tcagtaacct ggttttttat aacaatttat taaataatat tcaatttata gtaaatcttt
    22501 ttagaattca agaaatatga aaaacaaata gtaatagtgc aagagtttaa tatatgcttt
    22561 ttaatttgaa attttattta ccttgtgaaa gtaaaaaaat ttaaattggg gaaatacatt
    22621 atgatatatt taacagagtc aactgattag tattaatatt tttctaatta ttatatttcg
    22681 aaattttttt tattaaatgt tatgattatt taaaaaatgt ttgtaatttt gtataataat
    22741 tttatgtatt tgttttaatg tatatttaaa taatagcaat taatcgatta atttattttt
    22801 tataatttat ttcatatttt tttagattaa atattatcct aataaatttt aaaataatcc
    22861 cccccttaaa aatatataac attatgaaaa aattattttt aatttcaatt ttgattgtca
    22921 tatcaatgac aatgtatggg caaaaagcag ccctacacac agcatcagga gtacaaatct
    22981 tcaatggctc caatgctttc gttaatgcac acaatgcagc tagcagtggc gatactattt
    23041 atctctctgg aggcacattc tatgcaccaa caagcattac taaaacatta tatatttatg
    23101 gagccgggca ttatctagac tctactcaag caactggtaa aactattata agtggtaatt
    23161 taatacttga taggaatact gatggcacat acatagaagg aatagacttg acaggcgaag
    23221 ttagattaca atttagcact cctgatacta ttagcaatat aactcttgct tattgtaaaa
    23281 tatctggcaa tattaattta agctataacg gcaatactta tagcaataat tttgcactcc
    23341 agcgtagtgt ggttataggc agcataaatt tcacacgcgc tactaatgct gctgtattca
    23401 atagcatctt acaaagccaa tgcaatagtt ctaatggtgc actttatgaa aataatattt
    23461 tgttttttgc aatcgcatct tatagtagcg gttttactat tattggtgat aataacatta
    23521 ttaaaaacaa tatatttttt aaaaatgaca ataaatctgg agttggtgga aatggaaatc
    23581 agttttttaa taatctattt acaactgcta caccatattt tggtgggtca tatactgcaa
    23641 gtggaaacta ttatcctgta aatatagatt ctgtttttgt aaataataat atttatactt
    23701 ttgattattc taagaattat cacttgaaac atcccgatac atatataggc actgatggta
    23761 cccagatagg catttatggt ggcacattcc catacaaaga aggtgctgtc ccatccaatc
    23821 ctcatataca actgaaaaat gtagctccta caagcgactc taatggacag ttacagatag
    23881 aatttaaaat aagtgcacaa gatgagtaat ccaagctact ttttcagtac aatactaaag
    23941 acattgtctg tattattgtt tttagcaatg tcgcagagta tctatgctca gcaaactatt
    24001 gtggaatatg agtattggtt taatgatgac tttaataata gaacaattac tgctattaca
    24061 ccagtatctc agtatcattt agatacaatg atatctgttt ccacccttcc taatggtata
    24121 aatgtgataa acttcagagc taaagattcg caagggaaat atagcgttat cacaagccaa
    24181 cttttttata aaaaagaatt aacagactcg gatactaatc acctactttc ttatgaatac
    24241 tggtttgatg atgattttca aaataaaacc gtagtagata tttcacctgc tgagcagaca
    24301 acagtattag cacaattaga tgtgagtacc atgagcaatg gtatgcattt atttaatttc
    24361 agagcaaaag atgaacgcgg atattacagt agcatagcat atcagttttt tttcaaaaaa
    24421 acagtaacaa gttcagatac taattacatt gtatcatata aatattggta cgatgatgat
    24481 tacaataatt taatctatgt ggatttacct acgccaacaa aacaacttaa tctaattgat
    24541 aaccttgata tgacgaggat accaaaaggc attcacacaa ttaattttca atttaaagat
    24601 gatgcaaact tttggagtgt catttctact gatacaatag aaaagatttc tctgccaatc
    24661 gctgattata cttatgaagt attattatct aattgtgatt ccacagttat agcatttaca
    24721 gacaatagca ttgatgggga catgtattta tgggactttg cagatggcac tactgataca
    24781 ttagccaatc caactcatac attttataat tctggcattc attctgttat tttgacaatt
    24841 actgatacta ctacactcgc tgatagcact aaacaaatgg atattttaat tacaggccat
    24901 actgcaaatt catttgcggt tatgaattgt gacagttaca catctccgag tggtaattac
    24961 acatatacaa catctggcgt ttattacgat actattccaa atcattgggg atgcgatagt
    25021 ttattaacta ttaatcttac tataaactat tcagataata taacagacgt tatcacagca
    25081 tgtgattcat acacatggat agacggcaat acttacacag caagtaacaa tactgctact
    25141 tttacattga ctaatgcggc agggtgtgat tctattgtat cgcttgatct caccataaac
    25201 tatactcctg ataatacaat tacacaaatg ggggatactt taatttctaa tgcagatgga
    25261 gtaaactatc aatggctaga ttgtaacaat gatttctcac caatatttgg tgagaataat
    25321 caaaatttca cgccaacaga aacaggaaat tatgctgtgg aagttactaa ttatgaatgt
    25381 atagatactt ctgattgcat aaatgtaata attgataata ttttagaaaa tacatttgca
    25441 gggaaaatca aaatttatcc taacccgtca gacggtagtt tttatattga ccttgaaaat
    25501 tattatgata aaatctctat ttcacttgct aatgtgattg gcgaaattgt ttatcaaaac
    25561 aattattata atacaaggtt aattttgata aacattgaaa atgcaaaagg tatctatttt
    25621 atttctataa gttcagcaga tgaaatagca acatttaaaa ttattttgaa ttaatccata
    25681 aaaattattt ataaattaat atttttggtc atttttaagt agttatttac gtatttagta
    25741 aaaaataaaa gattatttcc ataaaaacat ataagaagaa tgtattaata aaacacttaa
    25801 tacttaaaaa caaaacatta ctattaagtc aagaattata aaaatattaa aaaacaggca
    25861 ttaaaaaaaa ttataatttt gtcaagaaat taattattta tgaatctacc tgtaaatatt
    25921 aattataaag aattctttga ttttataaag caatttttga cagataatag attggaaaga
    25981 ttttataatg tcataaatga tagaattact aatgtacaaa taatattaga ggatatatat
    26041 caagggcata atgctagtgc aattatacgt acctgcgatg ctatgggtct acaatatatc
    26101 aatgtcatcg agaaacgaaa taaatttaaa cctaataaag atatatccct gggtagtgat
    26161 aagtgggtag atataatgac ttatagtggc gaaaaaggac ttaatgattg cattaaagtc
    26221 ctcaaacaac gcgatttcac tatcgtagct acatcatcgc atttgcccga agcatatact
    26281 ccagaaacat taccaataga taaaccaata gcactaatct ttggcgtaga aaaagaaggc
    26341 ttatcagatg aggctatgtc attagcaaat gaatatatgt atattcctat gtatggtttt
    26401 gctgagagcc taaatgtatc cgtatcagta ggtatctcat tatttagtat tacacatcgt
    26461 attagaaaag ctgacaaaat gttaaaaccc tgtggtgatg aatattacaa gattttatgc
    26521 aaatgggcat tagctacatt atcaaattct gaattttata taaaaacttt cataaacaaa
    26581 taaaatcatg ccaaataata aagtaagaaa taaggctttc acttttttac tattaatggg
    26641 tattgtaagt ttattagcag acatgactta tgaaagtgcc cgtagtttga taggtccgta
    26701 tttgttattt ttaggagcta gtgcatctgt attgggtttc gtatttggcc taggtgaatt
    26761 tatcggatat acatttagat tggtatttgg ttttgtagtg gataaaacta aaaattattg
    26821 gctttttact attttaggct attctattaa tttaattgct attccactct tagctattgt
    26881 accgcctaat ggatggatct gggcagctat attaatcata tgtgagagat ttggtaaggc
    26941 ggtgagagca ccagctaaaa ctactttagt atcattcgca gctaaacgtg taggcacagg
    27001 taagggtttc ggatttgtag aagtgttaga ccaaatagga gcaatgttag gtcctttatt
    27061 tttatttttt attatgcaat ggaaagaatc tgctggcaca catgctcaat atcaatgggc
    27121 ctttgctagt ttggcagttc cagcagtact cacactaatt gttttattta tcgctagagc
    27181 aatcttccca aaagcagaaa aatttgaatt aactgataaa aaaactaata tcacttcaca
    27241 aaaattaaaa tttaaagaaa tcaaacctat attattatac attattgcta taggattact
    27301 agcaatgggg atggcagatt ttcctttgat ggcttttcat atcgaaacta ataaagtgat
    27361 agatgtcaaa tatattactt tgatctatat gatagtaatg ggagtagatg gactatcagc
    27421 ctttattttt ggcaatttgt atgataaaaa aggtgctatg acactcttga tatctgctat
    27481 tattggtttg ttatttgctc ccttggtttt tgtgtcaaca aatcttacaa gcattgtcat
    27541 aggattgata ttgtggggag ttttgcttgg tgctatggag agtgtagcct tagcttatat
    27601 agcagataat gtagaagtag cttatagagg tagaatatta ggtatatatt atacttttta
    27661 tggcattact tggtttgttg gaagctggtt aatgggaatt ttgtataatg taaatcctgt
    27721 atatgtagcc attttttcaa ttgtaatatt actatctgct ataatagtac tatggcgaca
    27781 aataaaaata aataaacaat gagaatattt tgtgatctcg atggagtact tactgatttc
    27841 gatggtagat tttttgaatt atttggtaaa tatcctaatg aatttattga cacatacggc
    27901 tattcttcat tctgggcatc ttttacacga caggcagaag acttttggag tggtattaaa
    27961 tggaagaatg atggaaaaaa attatggcag tttattcgtc catacaatcc aattatctta
    28021 tcttctgtgc ccccctccat ggcaaaaata gctactaaat ataaggaaaa atggattaga
    28081 gatgagttgg gtttagatat agaatattat attacaagta gaaaagaaaa acaaaattac
    28141 agcgaccaag gaagcattct aatagatgat atgaaaaaaa atattgacga atggttactt
    28201 cgtggtggta ttggcatatt acatatttca gcagaaaata ctattaaaga attaaaaaaa
    28261 atcggactat aaccttctct acacctcgtc aatattttta ataatattaa gatctacatt
    28321 ttcaccttct tttattgcta attttgcttt ttttgaatat tttaataata aatcttttaa
    28381 aatatcttta ttacctttta aaacaattcc gactggagat ggttcgctca agcaatagat
    28441 gtcataatta gaataggcta tttcataaat ttttttattc tcctcataat ctctacctat
    28501 cattatccat gtatcatctc taataaaatg tctgccaaat gtaataagtt cagctgttat
    28561 aggattaaaa tcaaatgggc cctttaaaac tttcaaaatc ctatcgccaa taactggatc
    28621 tgccaataaa caaccacctg ctgggctagg tatatctttg ataccatatt tttctgctag
    28681 cattagctgc tctttgcgag atctacccga tatagctaat aatttatctc tatccaccaa
    28741 accatccctt tctggcttag tagggaaaag taactttgca gataatggtc gaagtaacaa
    28801 atcctcagct ttagattttt gctttattaa tactaatgca ttttttgttt gactcattgg
    28861 cctctgacct atcacttctc ctgtagctat gaaatcagct ttttcttctt ttagcagttc
    28921 acaagcttca ttaatcatta atattttaca atctatacaa ggattagcgt gggagccata
    28981 tccatattta ggttttttta ataattttaa atatttttct gtgatatcta taatcttgcc
    29041 ttcatcaaat tcgtaatctt ctattttttt taaaaatttt tctggttctt tttttaattc
    29101 ccacccaaaa aatggcgtaa tatatttcaa tgctgttaca tatattcctt gctctttaag
    29161 caattttccg gctaaaatac tatctaaacc accagaaaac aataataaag ctttagtcat
    29221 aattacttaa ataaaaagac tatcaaaatt aataaaaaat tataaatttc aaatctataa
    29281 catgcttatt ttcatacttt cgccacttta tatttttttt cagtttttta ttaacatttt
    29341 ttttaataaa gtataagaaa gtaaaaaaaa tccgttaaat ggtaagtatc aaatggaaaa
    29401 cgaaagagtt attagaaaat taataattgt tttacttgag gtagtttatt tatatttcat
    29461 attcactagc cgccgatact catcctatgc ccatctgtgc gaggttgcaa agttttaaag
    29521 tcgaccaatg tctgattcaa tcacgctcgt caagtactta catgtggtta tagaccacat
    29581 ctaatagctc taaaatagca ttagaattat ttggtaaaca ttcctctgaa tcattgcaac
    29641 caaacaacgg taacaaaata actatattaa aaatatagta taacatactt aatgtataac
    29701 aattatttat tttagcaaat ctcgtttcat ctaccacaaa tcgattttaa ctttaataga
    29761 gtcaatataa ttattattta ataagattta cataatttcg aataattcaa gatttccaga
    29821 ttttatctaa aatatttaaa aattcatatt ctaaatttgg tgtaaaaaat tgtttaccta
    29881 gttgagaaaa gatttctttt tttgtccacc acctcagttc attaacttct ttaccaagtt
    29941 ttaattgatt ttgatctaat attttagtaa caaaaacaaa aactaattct ttttctattt
    30001 gcgactccca aatataatga tgaatgagct gagggataaa tttttttaaa cctatttctt
    30061 cttttgcttc tctaaataaa gcatcttctg gtttttcatt atatgcgata tgtccaccta
    30121 cactagcatc ccatttgcca ggttgaattt cttttttatt acttcgcttt tgcaaaagca
    30181 aattaccctc attattaaag acatataaat gtactactgg gtgcaacatt ttagatccat
    30241 tgtgcacttc atctcgatta gctacattta ttatttgtaa ttgatcattt aaaataggta
    30301 caagctcttt ttttctataa ttcaaattta ttttgttaat aattaattgt gctatgagcc
    30361 agactcccat tataatatat agtaatacac cactaataaa tgcccaccat tcatgtgata
    30421 aattatatat agaaaataca attaatgcaa tatgcaatgt aaaaagtatt aggagtgtta
    30481 gtaagttttt tcgaaattct attgtttgct cttctgttat ctctggtatt tccatgtaat
    30541 gtcccatcca tttgaaaagt atgtttatat tagtaaaata aataattgct aataataaca
    30601 ctagcaaagt ttctattagt gctggtttcc ataaaaaaaa tacttcatta tcaaaaatta
    30661 ttgatatacc accgagtaat gacaatagta cagtgtctaa aagaataaat ttatcccatt
    30721 gtttttttat tataccaatt aaaacaaatt gtattatacc aaatgatatg gctacactaa
    30781 tggcagtttt aggtggcaaa aaactatcta ttattatata aacaagtaat ggtaatagac
    30841 caggaagtaa ttttaataaa attttcataa tacattaaga gcggtaaacg ggattcgaac
    30901 ccgcgaccct cagcttggga agctgatgct ctaccacctg agctactacc gcttatttag
    30961 atatacaaaa ttaataaatt tttttacatt tttatataat tttgctcata aataatttta
    31021 aaaaacattt atgaacttta ctaaatcgcc agattggcaa agggaacaca atttttataa
    31081 agaaaaaaaa actgctgaac aaaaaacttt aattgttatc ataattacat ttataacaat
    31141 gttagctgag ataataacag ggtggatatt tgggtctatg gcactttttt ccgatggatt
    31201 acatatgggt acccacgttt ctgcactcag tatttctctg ttttcttata tacttgctag
    31261 aaaatatgct aaagatatta gatatacttt tggcacatgg aaatttgaaa tcttaggtgc
    31321 atacactagt gcattaattt taggtttaat ggggttattc gttttaatag tttctattaa
    31381 gcgttttttt aatccagtta agatcagcta tgactatgcg ttactagtag cagttattgg
    31441 tttattggtt aatgttgtgt gtgcttttat tctacgcgat aacaataagc atgagcataa
    31501 taaagattta aatctcaaat cagcttatct gcacgtaata gttgatgcgt tgacatccat
    31561 tttagctatt attgctttgc taggagccaa atattttaat ttgaattttt tagatccatt
    31621 aatgggattt gttagttctt ttttaatttt tagatggact tattttctca taaaagattc
    31681 atctgctata ttactcgata agcaaacaaa tccaacatta gtagaaaata ttatccaaat
    31741 tatagaatca gatggcaaaa gtaaagtaat agatttacac ttgtggaaat tgacttctga
    31801 taaatacgct tgtatagtat ctatagatac ttcaggttta tatagtatat acgaatataa
    31861 agatcgatta aaaaaatttg aagaattagc acatataaat gtagaattac ataaaaataa
    31921 tggtaatctt agaacttctt aataaattta ctaataaaaa attattttat ctttactgat
    31981 attaatagct agtttttttt gtgtacttat ttttatttta cagattcaaa aaaaagttta
    32041 taatatttta tatttttgca aatgaaaata aagtaatatt ttttctaaaa aaaatcatat
    32101 atgagaatag ctgtaaatac aagatttttg ctttatcaaa gattaagtgg ttttggcgtc
    32161 tttattgaag agatctttag tagattagta aaagaacatc cagaggtaga atttactttt
    32221 atatttgata ggccttatca ttcatcgttt atttataata caaatgttac tcctaaagtt
    32281 ttgtatccac ctgctagaca tcctatatta aaatatattt attatcaaat ctcactcaaa
    32341 cgttatatta ataataataa atttgattta tttatttcac cagattcaga attgccatta
    32401 catttaaaat gtccatcatt attagtatta catgatctta attttatgta tgatcataag
    32461 tatttaccat tatctgatcg acggtatatg ttgtatttta cacctaaatt tttaaaacat
    32521 gctacacgca ttattactgt aagtgaattc agtaaacatg atatagaaca taattttcca
    32581 tttactaata ataaggtagg tatagtctac aacgcggcat ctgaaaaatt taagccatta
    32641 tcagaacaaa aaattcaaaa atttagagaa aaatattcaa aaggtaaacc atatttttta
    32701 tttgtaggag taataactcc gagaaaaaat cttgcaaatt tattaaaagc ttatagtatt
    32761 tttagagaaa tgtctgatat agattatcct ttaataattg gtggcaataa aattcataaa
    32821 tataaagagt ttgaaaaaga attaaaaaat aataaatatt cttcagatat tttcttaata
    32881 ggcaaggtac tggatgaaga aatagcaagt ttaattggta gtgcatattc cttagtattt
    32941 ccttcaaaat tcgaaggctt tggtatacct ataatagaag ctatgaaatc aggtatacct
    33001 gtcatcactt ctaatgtaag ttctatgcca gaggtagcag gtgatgctgc cttattagta
    33061 gatccatata atcctagatc aattgccgat gctatgttat tattaactaa atcaaatgat
    33121 ttgtatgttg atctaaaaaa taaaggatta gagagagcta aatcattttc ttgggacatt
    33181 tctgctaaaa aaatgtggga agaaatagaa aaaactatag gtatttcttg agatatctta
    33241 attttcagaa ctttatatct gacattaatt acaatatagc gagctatatt aaactaaatt
    33301 attacaatta ttaaaataaa aatttttaga ttaatcatca aaaaagatga gggtcagaca
    33361 taataaattt tatcaattta gatacagctt tagctctatg acttatttta tttttttcat
    33421 aaatattcat ttcagcgaat gttttgctat aaccattagg aatgaaaata ggatcataac
    33481 caaacccatt tacaccactt tgatactccg cgatatgtcc atttattata ccttcgaaaa
    33541 aattaacttt atcattaatg ataaggcaaa taactgttct aaatcttgct tctctttgtt
    33601 ttattccttt taattcattt aataattttt ttatatgagg atcgaagcta ttatacccct
    33661 cacctgcaaa tctagctgaa aaaacacctg gtcttccatt taaagctttc acttctaaac
    33721 ctgaatcatc tgcaaaaaca ggcttattat atatttgata aattgtattt gcttttatga
    33781 atgcattctc ttcaaatgtt tgaccagttt catttatttc tcttgaatat cctatatcat
    33841 ttaaacttag aattttgata ttagaaccta tcaaattttg aatttctttc aatttctcca
    33901 aattatgagt tgcaaaaatc aattccatat tattttttaa attaactaat gaataaaaat
    33961 taatatatga ttactactag tatttaaaat agatttatta attatttttc gttatattta
    34021 ttgattaatt tcacaaaatc aatcaaacaa tgacatagta tcgtcactac ctttatattt
    34081 aatacccagg tgctcataag ctaatttagt agcctcacgt ccacgaggtg tgcgttttat
    34141 atatccttgt tgaatcagaa aaggttcata aacttcttcg atagtaccag gatcttcact
    34201 tactgctgta gctatagtat ttaagcctac ggggccacca ctaaactttt gaattatagt
    34261 tgttaaaatt ttgatgtcca tctcgtctag gccgtgtgaa tctatattca aagcattaag
    34321 agcgtagaga gttatttgtt tttctattat accagatccc ttaatctgtg caaaatctct
    34381 cactctgcgg agcaataaat tagctatgcg aggcgtacct ctactacggc gagctatttc
    34441 ttctgctgca ttaatattta tctctacatt taatatttta gcagagcgaa gaactatctg
    34501 aagcaagaca tcagaagtat aataatctaa tctagaattg ataccaaagc gagaacgtaa
    34561 aggagaagta agaagtcctg atctagtagt agcgcctatg agagtaaatg gattcaattt
    34621 tatttgaata gatttagcat tgggaccaga ctctataagt atatcaattt tataatcttc
    34681 catagcagaa tatagatatt cctctactac ggggctcaat ctatgtatct catcaataaa
    34741 tagaacatca aaaggtttca tagaagttaa taatccagcc aaatcgccag gtttttctaa
    34801 tacaggaccg gaagttactt ttatatctac acctagttca tttgcaatta tgtaagacaa
    34861 agttgtttta cctaatccag gaggtccatg tagcaaaaca tgatctagag cttcattgcg
    34921 agcttttgct gcttttacaa atacacgtaa gttttctatt atttgcgttt gaccaaaaaa
    34981 ttcttcaaaa ttctttggac gtatcaagct ctcatattct cgatcttttt cacttagttg
    35041 atttttatct atacttaatg catcatttct agacatacta aaatggataa gtttttttca
    35101 aagttactaa ataatatgaa attaataaaa aattcatttc atcttttgtt ttcaaaaaaa
    35161 ctcttcatta atttaatggc atcatctttt aatataccat ttttaacttc agttttttta
    35221 tgaaggattg aaggatgaaa taatgtatat ccatatttga tatcagaagc ggcataaata
    35281 attttactga ttcttgtcaa agacaaggca ccagcacaca taggacaggg ttcgatagta
    35341 acatataaag tacagtgatc taaatattta ttattaaaaa attctaaggc agttgcaata
    35401 gctatcatct ctgcatgtgc tgtaggatct tttaatctct caacttcatt gtgagcttta
    35461 ctgattattt tattattagc tacaataata gcacctatgg gcacctcacc gtattcaaaa
    35521 gctttttgag cctctttgag agcttctctc ataaaatatt catctcgtga tatcattaat
    35581 ttattttttt attaaaacta tttccaccct acgatttttt tgcctacctt cattagtttt
    35641 gttagtgctt ataggctttt tgtctccata acctacatac tgcattctag caggagatat
    35701 tcctttatta aaaaaatatt tataaacagc ttttgctctc tcggtactca accatttatt
    35761 ataaaaatcg ctaccaatgt tgtctgtatg accattaatt acaaattcta tttcttgata
    35821 agtttttaaa aaatctacta ttgaatccaa ataaattaaa gaattagcag ataattcata
    35881 actatcaaat gtaaaatata cattatctaa tacaaaaggt tgattcaatg gcaaaacatc
    35941 taattttttc cagatattag ttttagtaat taaagatact tctaagaagc tatctgtttg
    36001 agtacatata gtattaatga tcaaatcatt aggatttttt aattcatata atttcacatt
    36061 gtcaataaaa cagtaagcat aataatccat ctcatttatg gatttatttt ttcttattat
    36121 tttggtattt tgtaaattat atggcacaaa atttcctata gtaatatact tttcatcacc
    36181 cgaagctata aaaaaaccca caaaatccat ccattcttga ttatcaatta taatactatc
    36241 atgcaaaaat atctgcggtt tataaaatat atactcatta tattcattaa aatctggtaa
    36301 cgacgaagat agataaacac ccaatccatt agtaataatt ccggaattag gagctgattt
    36361 atatgaaaat gataaagcat aataagaatt ctttttaagc gatatagcta tctcagtgga
    36421 tatatgctct acatatcctt tattatattg ataagaggct gtgtctactc ttagtattat
    36481 tccgacaaaa ccatccccag acttaggttc agaacaacct gcaaaattgc atggaacgct
    36541 cacattagta cctttagcgc atctatgaaa aaaatcaggt gttgacctat taataaaata
    36601 ccaaaagggt aaaaaaatat cattagtatc tacatttaaa tatttagtat gagaattagg
    36661 gcaacgtgat attatttcga aatcgccatt tggcaaaaga tttttttgag caattaaaaa
    36721 atatgaaata aaaataatta aacaagtaat taattttttc atcgaatatt tattagattt
    36781 tttaagttgt taaacaaatt taaaaattat ttttcacatt ttctatattt ttcattagat
    36841 attaaattgc attcactgaa aaatcagcat ttatattaat ctatattgaa atgatttttg
    36901 atatataatt aaattttgag tggagaatca gtcaatataa acgaatgtaa tgtggtgagt
    36961 agatataaac aaatcattgt tttacttgaa gtagtttatc tatactttgt attcaccagc
    37021 cgccaatact catcccacgc ccatctgtcc gaggatgcga ggtttaaaag ccgaccaatg
    37081 tctgattcta tctcacttgt caagtattta tgtgtgatta caaaccacat cagatagctc
    37141 tggagatatt tagtagccac acctctaaat cgccgcaacc aaaccttgaa atcgtttata
    37201 tgcttatcga ctactttata cactgtatac accgcgacta attatttaat tatactataa
    37261 aatacgtaaa gtaaaacaaa tgataattgc ctctaatgcc cttacaaagc ataatattta
    37321 aaaataactt actggtatat ttttagaatg gagcttttct tagtgcaaaa tgatgcccca
    37381 tagcaatagc taaaagtgtc gaaatcatag gatgtgaaat aggattatta gtaatagcag
    37441 ctatcatcat tgataatcca aataataaat aaaatggagt aatgaatatc ttgaattttt
    37501 tattttcata tttaattatt ggaaatagat accaaaaaat ataaagtatt ataccaatga
    37561 taccatacct ccacaaataa agtatatatt cattctctga atatattgta taagaataaa
    37621 aaaagttttt atatggtcca taaccaaaga ttggtttttc tttccacata tcgattaaaa
    37681 acttccaaac atctaagcga cctctcacag attgattggt agatacaata ttttctccac
    37741 tctccacaat atttttgaaa aaatcagaaa aataaataat tgaatattta tttgttatac
    37801 taagtataaa aaaaataaaa gccgttccca ataaaataca gattaaatat ttaataggtt
    37861 tatgataaaa aataaaataa attattggta taactataat aataactaaa gctgtgcgag
    37921 attgacataa aataatagct gtgatagata aaaacaaaaa tatccattga aaatacttaa
    37981 tcttttttat tttttcatca aacatagacc ataaaaacca gcagaaataa aacaaaaata
    38041 tcaatgcatt attattagga ttgcctgctg tgccgagcat acgtttggga cctggctgcc
    38101 ctagtgaatt tttaccaaaa gtagaccagt gtaataaaga aggatcataa tatggtacta
    38161 ttaaatcatg aaaatgaaaa atattgaaat attggaataa attaataatc aataataata
    38221 taaatgtaat ctggataaat aaattaaaat gatgtggaaa atatttaaaa taggatatag
    38281 ttattattgt tatgataata aattttataa ttttataaaa ttcaaaaaaa tcattataag
    38341 aaacattaga atgattaaca atcaaacaga tggctatcca taatgcgaag acgataaaaa
    38401 aaataaaaga ctttggaatt attcgatttt tatcaataat aattaataat agacttaatg
    38461 gtacaaatat atctacaaac ctaaaataaa tacccaatcc aatatcaaaa gggggtataa
    38521 aaatattaga aataaatatt aaataaaagc aaatagcata aaaaaaaggg aaattaattt
    38581 ttatagattt aatttgcata aaaaaatttc aaaaacaaaa ataaacaaaa aaaaaccgtg
    38641 aaaaatttta gttttcacgg tcaaattata taaaatattt caaattataa ttaacaaaaa
    38701 ttctagttca aaaatttggt acctcgggcg ggacttgaac ccgcacggcc ataaataagg
    38761 ccacaggatt ttaagtccgg cgcgtctacc atttccgcca ccgaggccct agagcggaaa
    38821 acgggactcg aacccgcgac cccaaccttg gcaaggttgt gctctaccaa ctgagctatt
    38881 tccgcattta ttttaaaaaa attacatctg caaaattatt aatatttttt aaaataaaaa
    38941 aattttttac aaaattttat ctaatttaat tgtaaaatgt ctcataatag gagcttcttc
    39001 tactattcga taaccatgct taatctgatc acgtttttca taaacagctt ttaatacttc
    39061 gcccacatat cttaaatggt cattagaata gatgcgacga ggtatagcta gacgtaccaa
    39121 ttccaatgct ggataacgat cttgtttagt tataggatct ctatcagcta atagagttcc
    39181 tatttctaca cctctcactc caccttctac atataattct actgctaatg tttgtgctat
    39241 atattcttct tttggcactt gaggtagaaa ctttttagca tctacaaaaa tagcatgtcc
    39301 tccagcaggt ttttgatatg gtattccgct ctcttgtaag atatctccta acagtttaac
    39361 ttgcttaaca cgatagtcta aatatgtaaa ttcagtagat tcatctaaac ctacagccag
    39421 agcattcata tctctacctg ccatgccacc ataagtaaca aaaccttcaa acataacatt
    39481 aaatgtgcta gtttgttccc atattttatg atcacgcata gcaataaaac ctccaatatt
    39541 taccaatcca tcctttttag cactcatagt catgatatca gcataagata gcatctcttt
    39601 tactatttct ttaatatttt tatcttcata acctttttcc cttgtcttta tgaaataagc
    39661 attttcggca aatctagcac catctatcac tactaattta ttgaaatgat tagctacttc
    39721 gcgtacttgt tttagatttt ccatgcttac gggttggcca ccagcagtat tatttgtaat
    39781 agtaacaata ataaaaggaa ttttagatgc ttcattagtt tctaatactt taactaattt
    39841 attaatatcc acattccctt taaaagggtg aatgatatct gtatcaaagg cttcatcaat
    39901 agtacaatct attgctgtag cttgtctata ttcgatatgt cctttagtgg tatcaaaatg
    39961 actattacca ggaataatat ccccagattt tattaaaact gagaaaagta cattctcagc
    40021 ggctctccct tgatgtgtag gcatgaaata aggaaaacct aaaatatctt ctattacctt
    40081 ttttaaatta taatatgatg aagctccggc atagctctca tctccaagca tcaaagcaga
    40141 ccattgccta tcgctcatag cgccagtacc actatctgtc aataagtcaa taatcgtcct
    40201 ttcgctgggc aaattaaata aattataatg agcctctttg atccattgtt cacgttcttg
    40261 tttagaactc atattaatag gctctaccat ttttactttg tagggttcaa aaaaaatttc
    40321 tttcataaat caattatttt ggcaaagtta attgaaaaat tacatattat taaaaaaaat
    40381 ttaatatttt aaagttaaaa taagcttatg taatgatttt ttaaaaaacg aaataaataa
    40441 taattaagtt taaaactgtg ggataatatt tagataaaaa tcaattagtt cccattgttc
    40501 ttgagtttta ttaatatctt ttctaattaa accgattttt ttaacataat aatatctttc
    40561 gtaatctgac gtatcagtat atgttttaat atatttaaat acttttatat tattgtagca
    40621 acgttgacca acatctaaag aatctagatt tttttcataa attaaattat tataattata
    40681 gcctcttggc ttcatactaa aaaacttagg tccattacca tattggttta aaacacaagt
    40741 ttgataatct agataatatt cagcggttcc catgtataac atattagctt ttttacgagt
    40801 agaatatata aattgggaga aatattcata ttcgaaatct ttttctgtag attcgtaagt
    40861 acccatctcg ccccctataa cccttagagt atctttctca tttgacaaag aatccatata
    40921 tatccaaaaa gtacctatgg gaaaataagt ataatctttt atttcttgat ctaatggata
    40981 gaggactttt tcttcttcac agcttatcat aatcaaaact gtaaaaacta ttataaaact
    41041 aaccaatata tttcttttca taatttattt ttttcaaaat taaataaaaa atttaattaa
    41101 acataatttt aaaaattttt agaaattttt tcacagagtc caatatttga acttattaat
    41161 tttgcctctt aatattccct ttatatctac tactatacct ttaccatcat tacgtagcaa
    41221 ttgcgttaaa tcatcttcag tcaatttctc ataattttta tgttttactg ctaatattac
    41281 agcatcataa ttattaccag gtatatcttt tagttgatag ccatattctt tgtaaaattc
    41341 tttagcatca gccatataat ctacagcatc tacattaatg ccataagttt ttagttcaga
    41401 tatcatatct atgatgcgtg tattacgaat atctgataca ttttctttga aagtaacgcc
    41461 tagtaccaag acattagttt ctaaaatatt tttaccagta gccatcaaca ttttagcagt
    41521 ttgctcagct acataatatc ccatagcatc atttaccctt ctgccaggca ttatcacttg
    41581 tgagagatag tgatattcat aagctttatg caacaaataa taaggatcta caccgataca
    41641 gtgtccacct acaagtcctg gttcaaattt cagaaaattc catttagtac ttgctgcctc
    41701 taatacatca taggtattaa tattcatttt attaaaaatg attgacaatt cattcattag
    41761 agctatattt atatctcttt gagtattttc gataatttta gcagcttcgg ctactttgat
    41821 tgtaggagct tgatgtatac cagcttctac tacccaactg taaatggtag tgatctgttc
    41881 taaactttct ttatcgcatg cagatacgac ctttatagta ttagataaag tatgatttgg
    41941 atcaccagga ttaattctct caggcgaata tccatatttg aaatctatcc ctgctcttaa
    42001 accactgata ttttctatta caggaataca atcttcttca gtagcacctg gatatactgt
    42061 agattcataa actacataat cacctttttt gattacttta ccaatagttt cactagcttt
    42121 aataagtaga ctaagatctg gaaaattatg tttatctact ggtgtaggca cagtaataat
    42181 ataaaaagat gcattatgta aatcttcata attattagta aagtctatat cacaattatc
    42241 aaatgcttca gatggcagtt cattgcttgg atctacatgt ttttttaata attctactct
    42301 atctggcttg atgtcatagc caataacatg tacacattta gcaaaagcta aagcaatagg
    42361 taatccaaca taacctaaac ctataacccc taatgattct cgtttagatt ttaattcttc
    42421 tgaaatcata attctttaat tatttgattt gcaatagggt ggagatcgcc atgtttagca
    42481 tttggtgtac tattgcgtat aaaatgtgct aattctatcg atgcccgagc atcctcgaga
    42541 gtaaaacctt taccagttaa aatttcttta taactctcgg tatgaagctc agtaaatcct
    42601 tcagaaaact caaattcttc attatttaat aaaatagacc gataagttct ttttccttta
    42661 ttaatagctt gttcgggtaa atctgatgcg ttaatggaaa gaaaccatct aacgcgcgct
    42721 ttttctaatt ctagatatcc agcagctcta tcgcgcgtat gaaaatgtac ataagaatct
    42781 tttacactac caaaaatcca taataacata tcaaaaaaat gaataccaat attagtagct
    42841 ataccacctg atttttggat atcacccttc catgaataaa aataccactt gccacgagaa
    42901 gttatatacg taagatctac atctactttg cgtttatcaa ctgaagtttc tatttgtttt
    42961 tttaattcct tgatcttgtc atgtaatcgt aattgaagaa tggtattaat tttataccca
    43021 gtttcttttt caattatttg caaagcatca atgttccagg gattgaggac aaggggtttt
    43081 tcacaaatag cattagcttg atttctaagt gctaatctta tatgagcatc atgtaaataa
    43141 ttaggagaac agatgctaac ataatgaatt ttttctttct ctccttgtcg acgaagttta
    43201 tccaaatgcc tatcaaagcg ttcgggttct ttgaaataat cagcttcggg cgcaaacgaa
    43261 tcaataatac ctatcccatc ataaggatct aaaactgctg ttaataagtt gccagtatct
    43321 ttaatagctt tcatatgtct aggggctata tatcctgctg ctcctataat agcaaaatta
    43381 aatttattca ttttattcta atttatttta ttctaattac tttattatct attaattggt
    43441 acaattgatg actttcgggg cattctgcaa tgtcattatt attaaaaaat aatttatgac
    43501 catattcact catccagcca atttgtttag ctggtgttcc tacaactaga gcataaggtg
    43561 atacatcttt agtaaccaca gcgccagcac caataaaagc atattcacct atctcgattc
    43621 cacaaacaat ggtagcatta gctcctattg tagcgcctct tctaataata gtagtttgat
    43681 attgatcttt tctgactaca gcactacgag gattgataat attagtaaaa accatgctgg
    43741 ggcccaaaaa tacatcatct tcgcaaacca caccagtata aatagaaaca ttattttgca
    43801 cttttacatt tctacctaat ctcactcctg tagctacaaa aacattttgt cctaaagaac
    43861 agttatctcc tatcactgca ctagacataa cgtgacaaaa atgccaaatt ttagtacccc
    43921 tccctatgat agctccttcg tctactatag cagtatgatg tgaaaaatat tcctcattca
    43981 taatttattt atttataaat tctaaaactg attttgttat atattccaat tgctcttcgt
    44041 ctaattctgt atgcatgggt aatgaaatta cattttgagc taaatactct gagataggga
    44101 aataatcatt tgtttttaca tatttttgat aagctttttg catatgaatt ggcactggat
    44161 aatagatcat agaaggtatt tgcctctcgg caagaaattt gatcaattca tctctagaaa
    44221 aattatttgt ttgtaatgta tattgatgaa agacatgagt agatttagca aaacgttttg
    44281 gtatcttgag gtttgattca ttagcaaaag cattatcata ataattagcc gctttttgcc
    44341 tagccatgat ataatcatct aaatattgta atttaacttt caaaatagca gcttgtagag
    44401 tatctaatcg agaatttaca cctatatatt cgtgatgata ttttattttc atgccatgat
    44461 tagcaatata acttagttta tcagcaagct cgtcagaatt agtgaatata gctccaccat
    44521 cgccgtagca gcctaaattt tttgatggaa aaaaagaagt acaaccaata tctccaatag
    44581 taccagcttt catggtattg ccatctgcaa aattgaaatc tgcaccaata gcttgggcat
    44641 tatcttctat cacataaata ttattttctc tagctatatt cattattacc tccatatcag
    44701 cacattggcc aaagagatga acaggaatga tagctttagt cttatcagta atagcatttt
    44761 taatatcatc tacattaata ttaaaagtat cagggtctac atctacgaaa actggcttga
    44821 gtcctagcag agctactacc tctacggtag aaataaaagt gaaagtagga gttataacct
    44881 catctcctgg cttcaaatca agagacatta aagctagctg aagagcatca gtaccattag
    44941 cacagggaat gacatgtttt acatttaaat atttttttaa aatatttttt aaagcattta
    45001 ttgctgggcc attgataaaa gcagtagtat ctatcacatc ctgaatagca ctatctatct
    45061 cagtttttat ttttaaatac tgagtttgta aatcaaccat ttttatcggt ttcattataa
    45121 tatttttaca aaaatataaa aattttataa tatttttaca aaaatataaa aattttacaa
    45181 aaaaattatt aaattattca aataaaaatt aaataaatga ctgtaattcc ccctattaag
    45241 acatgtctat aacatttttg agaatctgta aatttttaaa aatattccta gtacaattat
    45301 caaaatattt cagataaata cgcaaaatta tattaatttt ttaattcatt atttgcaata
    45361 ttcttattat gtttgcataa aaaaaatatg tataaggatt ttcaagaata tttaatcaaa
    45421 gaacttgctt caatctacga agctggttta gcaaaagaag agcgtataat agtatcgccc
    45481 caaggggccg taatacgcct cgccgatggc aaagaattat taaatttctg tgctaataat
    45541 tatctaggct tatctaatca tccacgtcta aaacaagctg ccactaaagc actcgaaact
    45601 catggctatg gtatgtctag tgttagattt atatgcggaa ctactgatct gcataaggaa
    45661 ttagaaagaa aaatagctga attttttggt accgaagata ctattctcta tgcagcttgc
    45721 ttcgatgcta atgctggatt gtttgaacca ttattaactg aaaaagatgc tatcataagt
    45781 gatgctctca atcatgcatc tatcattgat ggagtgaggt tatgtaaagc tcaacgtttt
    45841 atctacgcta atggtgatat gaatgagcta gaagagcaac ttaaaaagac tcaaaatcta
    45901 cgtttcagag ttatagctac tgatggagta tttagcatgg atggtaatgt agctcctgtt
    45961 gataagataa ttgaattagc taaaaagtat aatgctatgg tgatgataga cgaaagtcat
    46021 tctgccggag tggtgggtga aacaggtaga ggtgttactg aactctttaa ttgcaaagga
    46081 caggtagaaa tgattacagg tactcttggt aaggcttttg gtggtgctat cggtggattt
    46141 actactggta aaaaagaaat tatagaaatg cttcgtcaac gtagccgtcc ttatttattt
    46201 agtaactcac tccctcctat gatagtggct gctggtatcg aaatgtttta tatgatagat
    46261 gaaactaatg aattacaaga caaactccat gataatgtaa agtattttat gcagcaaata
    46321 caaaaagttg gctttgatgt taaacctacc cagagtgcta tcgtcgctgt tatgttatat
    46381 gatgctaagg tggcttctgg tatggctaaa agacttagtg atgaaggtat ttacgtgact
    46441 ggtttctctt atccagtagt acctaaagat caagctcgca ttagagtaca actatcagca
    46501 gctcacgaat acgaacatat tgataaatgt gttaatgcat tcgaaaaagt cggaaaagaa
    46561 atgggaatat taaagtaaaa catattttag aaaatataaa tatatttgat aaaggggcaa
    46621 taattaatca ttgtcccttt ttataatttt aattgtttag tgttgttaaa tttttaaaaa
    46681 aaaaaataat tttttttgct attttaaaat ataattttat taattttgta aaaaaaattt
    46741 tttatatcac taaaatagta ttgtaaatgt atccaactaa aatagcatta gtaacaggtg
    46801 catcgtctgg tataggcaga gctttatgta ttcaattagc taaaaaagga tattttgtag
    46861 ctggcatgtc tagaaatatg gaaaaattaa ttagtctaca aaataaaatt aaacgattta
    46921 acattgaatt tttacctgta caaggcgatg taagaataga atatgattgc aaaaaattta
    46981 ttgatactgt aatagagaaa tggggacata tagatatttt aataaataat gctggtatat
    47041 ctatgagagc actttttgat gaactaaatt tagaagtgtt acacgaatta atgaatacta
    47101 atttctgggg tactgtatat tgtacatatt atgccttgcc ttatttatta gaagctaaag
    47161 gtagcttagt aggtgtatca tctattgcag ggtatcaagc attacctgca cgttctggtt
    47221 actctgcttc taaatctgct attcatggtc ttttgaatac tattagaata gaaaatttat
    47281 atactggatt acatgtaatg atagcttgtc caggctttac ctcatctaat atcagaaata
    47341 ctgctcttac tgcagatggt tctccacaaa acgaaactcc gttagaagaa ggtaaattaa
    47401 tgagtgctga gatggtagca aaaaaaataa taaaaggaat agaaaaacgt aaaagacaag
    47461 taatcatgac tacagaaggt aaattaacag ttttcttagg aaaatttttc cctgcttttg
    47521 ttgataaact tgtttttaaa aatttgctta aagaaaaaaa ttcaccatta ataaaaaaat
    47581 atcaatatgg aaaaaattaa aaaattagaa attatcccat tagaaggttc taatttattc
    47641 aaatttggag attcacaaga tctaatatta gaaacattag gagaacctga agatatcgaa
    47701 ctattcgaag atgaagatga ggatgaacct aataccagta tatggtttta tgaaaacaat
    47761 atttctttgt ttttcgatga agtagatgaa gactatttca tattaaaagc aattgaaagc
    47821 tcttatcctg aaacttattt taaaggtact aaaataatcg gtatgtctga caacgactta
    47881 aaaaattttc taaaaagcat aggttatgat aaatttgatg aagaaatcga agattggggc
    47941 gaaaaacgta tctccatcga agaagatatg atagattttt atatcgaaga tgatactgtc
    48001 attagcgtag cctggtctag tgaatattaa aaatatataa aaagaatttt ctttaaatct
    48061 atttttaata gtcatctcta ggaattataa gtacttataa ttcctagttt tgttttgatt
    48121 ttttaaaaaa ataaatgtaa cagcatattt aaaattccaa aaatattaca ctataaaata
    48181 gaatatacaa ttacgttgct tgttttaaat taatttttgt atttttttat tgtcaaatct
    48241 gataaaatat atttaatttt ataaaaaaaa agtgaaacct ataatagaaa ttgaaaattt
    48301 aaccaaaata tatagattgg gaaaattaca tggctcatat ttgactttta gagacgccat
    48361 cactcaagta tttaaaatta ataaaaacag gcaaaaaaga cagtatatca aggctctaga
    48421 caatgtaaat ctatgtattc aaaaaggtga aaagataggt ataataggca aaaatggtgc
    48481 aggcaaaagt acacttttga aaattttatc cagaataaca tatcctacag aaggtatagc
    48541 tattttgcgt gggagaacag ccagtttatt agaagtgggt acaggttttc atggagaact
    48601 aactggaaga gaaaatatct atctaaatgg tgctatttta ggtatgaaac gtcatgaaat
    48661 tactgaaaag tttgatgata ttgtagcttt ttctggagta gaaaaattta ttgatacacc
    48721 agtaaagcat tattcttctg gtatgcagat gcgtttggct tttgctgttt ctgctttttt
    48781 agaagctgat attatgcttg tagatgaggt attagctgta ggcgatgcag aatttcaaaa
    48841 aaaatgttta ggcaaaatgg gagaagtatc tactaaagaa ggtagaacaa taatatttgt
    48901 aagtcacaac ctaaatgcta tcgcaaatct ctgtgaaaaa actattctat ttgtagatgg
    48961 gcgagtagaa atgtttgact atactgaaaa agtaataaat aaatacataa atgaaatagc
    49021 tcaacatgaa acttctaaat attgggataa atttgataat aattatgtta gacctatttc
    49081 ttttaaaata ttaaattgtg aaaataaaga aaaatttaag tttcaaaata ttgaggatat
    49141 ttatttcgag atagtatatg aaaaattatc cgatgatgat acctatccta atattcatct
    49201 tttgacatct aataatcaat atgtattgac aagtataatt gatgataata aattacccaa
    49261 gggcaaggga atattcaaag tatatgctaa attagaaaaa gaattattaa accaaggact
    49321 atatcatgta gggcttgcat ttacaaattt taaaaaaaat cgagttgatt ttttcgaaaa
    49381 ttattgtatt agtttcgaaa tagtagatat acctgaaaat cgaaaatatc cttattttgc
    49441 tcctttatca ggtattataa gaagagaact aacatggata gtatgatttt tttttagtat
    49501 atttgataaa aaatattatt tatggatatt tttgaagcat tacttaccag gcgtagcata
    49561 agagaatata ctggtgaagc aatagaaaaa gaaaaaatta tagaagctct taaatatgca
    49621 atgtatgcgc catcagctca taatagtcgc tcatggcgtt tcatacttac atctaaaaaa
    49681 gagcatttga ttaaaatatc agaaatacat ccttatgcta aaatgctccc aaatgcatct
    49741 tgggcagtat tattttgtaa tgacttggat gctgaaccta cagaaggcta tggtgctcta
    49801 aacgtagccg cttgtataga aaatttttta ttagcaatac acggtatggg ctatggggct
    49861 gtatggatcg gtatatatcc aagaaagcaa agaattcaag ctattaaaaa attttttgag
    49921 ttgccagata atcttttgcc aatagctatt tgttctgttg gggtccctgc cattaaacca
    49981 acaacagtag atagatatga accagaaaaa attttaaaaa tatatgaata gtatcttaaa
    50041 aataactttt atttttccat tcgtgtcttt gcttgataaa ataatctcta agaaactata
    50101 atctcaataa aaaatttatt gagtaaatat agtaacaaat atacatatca aaccattaat
    50161 aaaataaaat attgatgata aaataaagaa aatcttgaca gagcttagtc gattagtttg
    50221 tttaccagca taagtaccaa caaataaaaa aagtaaaata aggacaatag tataaataaa
    50281 cgatcttgta aaattagtaa aaatatagct gacccccaaa gctgctataa aagattcgaa
    50341 actcatagaa aacgacaaag ctattacatt tttaaattta ccaatatcta ctattagctc
    50401 actcaatttg ccttttgcta tactcagata cattctgata gataaaaata aaaataaaat
    50461 gatacttata atagatgggt taaaaggcat accatatctc gtagctaacc ctaattggaa
    50521 acctaaaaaa tacattaaaa tagataaaat agcaaaaatc aaattaacat aaataatttg
    50581 aaaatttgat ttaaaaatca atgcatgcct ataagatgta gccatcaaaa aaaatcctat
    50641 gctaatgccc gctaaaatag gtattatatc cataaaaaat tatttacttg caaatctatg
    50701 taattttttt tacgtgacta tattttttaa attattaaaa ttattttagt cgtttatttt
    50761 gataatgttt attctttatt tctttgtgtt aaacaaaaat atgattctca atttttgaat
    50821 tagtcatata ttattatata ccatcacact atgatagcat agtattgtct gtaaatattt
    50881 attctttagt taatttgcca gtttttatta tttcgttatt gctataaagt ctaaaaacat
    50941 agttaccact acataattca ctcaaatcaa tttgaaatat ggtactgggg ggagcatctt
    51001 gttttaaaat taatttacca tcgagagaaa atatttcaat aattgcaata ggtttatcag
    51061 cctcgaaggt tataaaatct tgaactggat tgggaaaata tttgacatga gatgatattg
    51121 cttgcgttat attaagagac ggatctacat ttacccaaga tatttgagtt ggttgcagat
    51181 tattatttaa cgtaatagta gctaaggggt ataataaatt tttcatctga aattctatac
    51241 tattggattc taatgtatct ccatatacag gcatccaaga taatccatat aatttagcca
    51301 ataaagtagt agtatcttta ctttgtacta atgtttttaa agtttgaaaa tctccattag
    51361 gcaatttagt agtcccccac ccaacagcct ctcgctttac gtactttttt aattctacag
    51421 caacactatc tatatatatg ttatttattg tagtatcaat gcgcagttta gttttgattt
    51481 taggataact ctctactact gttcccattg tataagggaa tgtataaacg agctctggat
    51541 ataaatattt taaatacgcc gttatattga attcttcaat agggatagtc aaacctacga
    51601 tagctaagtt attagtagtg tgtgaaataa tagtataata agtattttct aaagaaatag
    51661 cataattacc attaggatat tgatctgcaa atgataaagt cgataaatca actaaatcaa
    51721 tacttagata atcattagga gttaaattag agagattcca cgtttgatta gctccattat
    51781 tagaaatatc aataccacct atgactgatg tggaattttg aataatagaa aatggaatag
    51841 gaggcatatc atcataagta atcgtgatct gtcccattaa catgcataca ctaagcatta
    51901 atgttaatag taaaatgatt gttaattttt tcataatttt aattttaata atttacacaa
    51961 aattaaataa ttttttatta gaaattaaaa aaaattattt aattttgtat ttacaaatat
    52021 ttttaaaaaa ataagattat gaaaaaactt ttatcattaa ttatcatttt ggtatgtagt
    52081 accattgttt atggacaaac attaatagta gatgagaatt ttgattctta tatagctggt
    52141 aataaatttg ttcaacaggc tggctcccct tggactactt ggagtaatca gcctggtagt
    52201 tcagaagatc caatagtttc tgatgcacaa tacgtatctt ctcctaattc tttatatatt
    52261 caaaataaca atgatttagt attaaaatta aatgcctata ctacgggtcg ttattcgata
    52321 tcatttaaaa tttttgtgtc tacaggttct ttgggatatt ttaatatact aaatagcttt
    52381 gctggtagta gtagtgtatg ggctatgcag gcttatttta aaaataatgg ttatctagta
    52441 gtagatgctg gtggtgcttc gatagatagc attgcttata ctcaaaatga ctggcatgat
    52501 gtcctaatcg tagtagattt agataatgat atagctacga tagaatttga tgaacaattt
    52561 tttcatctgt ggaaatttag tagcggtact tttggagatg gtaatatgaa aaaattagat
    52621 gctatagatt tctttggctg gtcagaaggt ggattaaatg ctaattttta tattgatgac
    52681 atgaaaatac aagaaatgcc tgcgccagaa atgcccataa atcttacagc tactttacaa
    52741 aatcaatttg atatacattt aaattggaca gctcccacta ctattcctaa ttgttacttg
    52801 cttgtacgca atgatcgatt agcagcgata attaatgcaa gcgaaactga atatatagaa
    52861 gctcatgtat atcctcaaac cgttaattat aagttattta gttatgtaac ttcacaaggc
    52921 gcatcagcac cagctttaac acaaataaca ataccagggg gcgtagatag aaattttgta
    52981 ttattcgaaa tagctactgc aacaaattgt ccatattgcc cgagtgctgc tagagggata
    53041 aatgatttaa tcaccaataa tgctaatgta gctgtaataa attatcatat cggcgatagc
    53101 tatgctacaa cagattgtaa tacacgttta tcattttata ctagccaagt aggaactcct
    53161 actacttggg ttgatggcgt tttaaatctt tctggcggtg ctactggtac aacaagctta
    53221 tattctgcgt ataatccaat gtatcaaagc aggatatcaa taccatcagt ctacattaat
    53281 tatataaata taaacaaagt aaatgataat caatatgaag ccaaagtatc agtaaaagaa
    53341 catacaaatt tttttacttc aggattaaag ctacatgtag ctttagtaga aagccatata
    53401 ccacaaacat ggtatacatt aagcgaatta aactatgtat gtcgcaaaat gtatcctagt
    53461 ggtaatggaa caaatcttga cttttcacaa agcgactctt tatcatttac ctttaatatc
    53521 aatgtagatg caggaatagt ggtatcaaat tgtgaattga ttgcttttgt acaacatcaa
    53581 cctactaaag aagttattca aaccattaaa gctccattag atccatcaag catcatacag
    53641 ttaaatacta ataatataaa tatatatcca tcaccagctt ccgattatat ttatgtatcc
    53701 gaagctcaag gtatgcatat gcaatttata aatctaaatg gtcaaatagt aaaagatcaa
    53761 ctaatatctt ctagcaactt tcctatttat gtaaatgatt tacctagcgg tgtatatata
    53821 gttacttttg ctgagaatcc gcagattaag caaaaagtca taataatgca ctaaactata
    53881 tacaatatta taataaaaaa aaccgactat tagtcggttt ttttttatta tttattttca
    53941 attttattat ctaaaatgct aatattatgt cccattttag ttgctttagt ttgcagataa
    54001 aatctattat aaggattagg ctctatctcg agaggtattg tctctactat ttctaaacca
    54061 taaccttcga gagctatttt tttaattgga ttattagtta ttaatctaat ttttttagct
    54121 cctaaagccc ttaatatttg tgcccctata ccataatctc tttcatcatc tttgaagcct
    54181 agagctaaat tagcatctac tgtatctaat ccttcttctt gtaaatgata agcttttaat
    54241 ttattcaaaa gtccaatacc tctaccttct tgattcatgt aaactataac tccttttcct
    54301 tcattttcta tcatttgcat agctctatgt agttgtggtc cacagtcaca tttataacta
    54361 ccaaaaatat caccggttac acaagaagag tgcactctta ccaaaataga ttcatctgct
    54421 tcccattttc cttttacaag agctaaatgc ggtaaattat ttgtaatttg caaaaaagct
    54481 tttaattgaa aattaccata ttctgttggc aaaaaaactt cttctacttg cttaatcaaa
    54541 gattcgtgct tgagtctata ctgtattagt ctttttacac tagttatttt aatatcaaat
    54601 tttttagcaa tttcaattaa ttgcggtaaa cgcgccatag taccatcatc attcagtatt
    54661 tctactaaaa cagcaatagg tttcaatcca gctaaccgac ataaatctac agaagcttct
    54721 gtatgtccag ctctttgtaa cacgccacct ggtttagcta ttaacggaaa aatatgcccg
    54781 ggtctaccga aatcttctgg tttagcatta tctcttgtta accaataaat agtacgagcc
    54841 ctatcagctg ctgaaatacc agttgtatta ccatagccta ataaatcgat tgatactgta
    54901 aaagctgtat tatgggtagc tgtattatgt tctaccatta atggcaattt aaatttatta
    54961 gcaacttcct gagaaatagg tgtacatatc aaacctctac cgtgtttaga catgaaatta
    55021 acaatttcag gagttatctt ttcagctgct aatataaaat ctccctcatt ctctctatct
    55081 tcatcatcta ctactattat caatttacct aatctaatat catctaaaat atcaggaata
    55141 tcataaataa tattatccat aattctacct catattatat tatgcaaaga tacattattt
    55201 ttcaacaatg taaataaaat ttatttagaa tgattttgta attaaactga gtaattgata
    55261 tggagggaat taataaaaaa aattttcata tttttacaaa aaaatatgaa aaagtattta
    55321 tttgctctat ttattttatt agcattttct attaataatt atgcccagaa tataaaagct
    55381 actgatacta taaataatga taatgaacct atctttgcac tagtacaaga acccgcaaaa
    55441 tatccaggtg gtgatgaagc tttattaaaa tttattcata aaaatataaa atatcctaaa
    55501 caagctatta aaaataatat atcaggcact gtatatgtac aatttattac agaaaaagac
    55561 ggctcaatat ctgatgtaaa agttataaga ggtataggtg gtggttgtga tgaagaggca
    55621 gtaagaatag taaataaaat gcctaaatgg atacctgcca aacaaaaggg tgtacccgta
    55681 agatatttaa atgtaatacc tattactttt aaaatcaaat aattatttaa acaaatcaca
    55741 taattatatt acaaataata aaaattatat tttataacac atagtgtaag taatcaaatt
    55801 ttagttttac ataagtatta gcacgtttat tttatttttt tatattgaaa aaaattatta
    55861 attttattat ttagatctaa aagccaattt ctaatgaaat ttaaactagt aagtgaatat
    55921 aagccaacag gagatcagcc agaagctata gagcaattaa taaatggtat attgaatggt
    55981 gagcaatatc aaatattaat gggagctact ggtacaggca aaacatttac tatggcaaat
    56041 gtaatagctc agttacagcg tcctacttta gtaataagtc ataataaaac tcttgctgcc
    56101 caactatatc aagaatttaa aaggtttttt ccagacaact tggttgaata ttttgtttct
    56161 tattatgatt attatcagcc agaagcatac ctcccttcta ctaacactta cattgcaaaa
    56221 gatttgtcta ttaatgaaga aattgaaaaa ttaagattag caactacatc ggctttaatg
    56281 tcaggccgta gagatataat agtggtagct tcagtttctt gtatttatgg attgggtaat
    56341 cctaatgatt ttttagctaa ttcttttcaa ataagactag gtcagcatat cagtgctaaa
    56401 caactaattg aaaaattggt gaatgctatg tatcaccgtg aagattatga gttaaatatt
    56461 gggacattta gattaaaagg tgatacacta gagataatgc agggttatgg agaaataatt
    56521 tataggatcc ttttttttaa tgaccaaatc gaacaaattg aaagctataa tggtatagat
    56581 tatacattat tatctattcc tgaggaggta actatttatc ctgctagcat ttttatgact
    56641 actaaagaaa aattatttaa agcaatagat cgcatagtat tagacctggg cgagcaatta
    56701 aaatttttaa gaaatcaaaa taagtttgaa gaagctcaga gattagaaga tagggtagtc
    56761 aatgatatag aaatgcttaa agagttagga tattgtagtg gtatagaaaa ttattcacga
    56821 tattttgatg atcgtaaacc tggcgagcgt cctttttgct taatagatta tttcccaaag
    56881 gattatttga tgtttattga tgaaagccat gttactattc ctcaaattag aggtatgtat
    56941 ggtggtgata gaacaagaaa agaaatttta gtagaatatg gatttagatt gccttcagct
    57001 cttgataata gacctcttac ttttgatgaa ttcgaatcgc taattaatca agttatttat
    57061 gttagtgcta caccaggaga ttttgaattt ttgaaaactg aaggtgctta tgccgaacag
    57121 ataataagac ctactggcat acctgatcct gaagttattg taaaacccac aaaaaatcaa
    57181 atagatgatt tactggctga aattaaaaaa gttagagaac atgatggtag aatacttgta
    57241 actacattaa ctaaaaaaat ggctgaggaa ttgtctactt ttctaacaga tcttaatatc
    57301 ccaactagat atattcattc tgatgtggaa actctagatc gtgtagaaat attaaaaagt
    57361 ttgcgtacag gtgattttga tgtattagta ggtgtgaatt tgcttcgaga aggtctagac
    57421 ctaccagaag tagagctcgt cgtaatatta gatgctgata aagagggatt tttgagaaat
    57481 tatcgaagtc tcattcaaac agcaggcaga gcggccagaa atgttaattc taaagttata
    57541 ctttatgctg ataaaataac tgattctatc cataagatga taactgaaac ttctcgtcgc
    57601 cgtgaaaaac aaattaaata taacgaagag cataatataa ctcctacatc tgtgataaaa
    57661 gagattagtt attctttgat ttctgatgat aataaaaatt atatttacgt taataccaat
    57721 gataatatga gtattgccga aaaacaagca gtatattata ctgaaagaga aattaaagaa
    57781 aatattaaaa aattagaaaa agaaatgaag caagcttcta aagaattaga ttttattaaa
    57841 gccgcttacc tacgtgatga aataataaaa cttaaaaatc ttttacgtaa aaacaaataa
    57901 taaactatta caaaacatgc ataagaaaaa atataaaagg caaggataga aagtttagtg
    57961 tagctttata ctctatcctt ccataatcta agtaatatta tttctgatat taatagtatg
    58021 atagaagaga tcacaaaaat caaccatata ggcaattttt tagtatgtat gattaagttt
    58081 gatgggccaa atgccacagg atatttacct attacaatat tattagaatt ttttattaaa
    58141 ctatctaatt cattttttga ccaatagcgt ggatcagact catgccttgg ataattaaaa
    58201 gctaagggta gtttattatc tttataatac aaataataaa tatcagctct actaattatg
    58261 ttttttaaaa ataaaattgg ctgctttgct ttgctaatga aaggtatata tttttcattt
    58321 ttaactagat ctaataaaaa aatagcctca tctgtacttt ctaatatatc acctcgagct
    58381 ggcagtgtaa atggtactgg aatgccagtc tcttgattga tataataata aagatctaga
    58441 gcttgagcag agctaaaggc tatctgcaaa attataggca caaatagctg atgttgaata
    58501 agcgtagtat actcactctg taatggggag gcaaatatat aaatttggcc tttaccatga
    58561 tttatttgtt ttagaaatgg atcattatta gttaagctaa gcaggttagt ccctttagat
    58621 ataggtaagc gataatgttt aaaaactaat ggaaaatcaa aatctcttgg agtactggta
    58681 aacatacctt taaaaaatgg actgtaataa gctatatttt caattctcaa ttgctgctta
    58741 tccataacat cactaaatga aatacctaaa tttgcaatta aagaattcaa ataattggca
    58801 ttttctttgg gtggtattat cagtagggca ttacctctct ctacctgagt caataaatga
    58861 ttaaatatat caggggatat attttttaga ctttctaata tatataatgt ggattgatga
    58921 ttagcagtta gaggcagctc atcaatatta atcttattat atttgaataa actatctcct
    58981 ttgaatataa aagatataaa agaacgtatc tcatcactag ctaaatgata aacctgaata
    59041 acggaaggga tattaatagc aaaaaacata gaattgtcga aatcataacc atcatccacg
    59101 atacttacct tgcctgtgat atagctgcca tcaatcactg gaaagctaat ttcttgcgtt
    59161 gtttgtgtat ttggtggtaa attagaaata gcagaaccta cagatttatt attaatatat
    59221 agatctattt tcactccctc ttctatatca ttattccaat ttttaagctt aatatataag
    59281 gttccttgag tatttttcac aatgacagga gctcccagat aaatactatc aatagatata
    59341 ttagtgatgc ttgatctttg tattggcatc aaataaacca tggattccat gctatcaagg
    59401 tattgaaaaa actgcaaggg aaagtctgtt ttttgaaaat cactaaatat gatgatagat
    59461 ttagctttgc tttcaataag tgtattcaaa aatgttttat aattcaaaat ctgtgaaggt
    59521 ctataagtaa tagaatcaat aaaatgcgta cattcatttt tgaggtgata atctggcttt
    59581 acactagtag atgtaggaat aatgctgata atggtagaag ctgattggtt agcaacagct
    59641 tctttagcta aatctttagc ttgctgtagc aaagtaaagg tgccattaaa agatctcata
    59701 ctgagactat tgtcgatagc tattaatagt ttgtctccac ttgtagtttc agtgatttca
    59761 gattttcgta caggcataca aaatgctaaa atcaaaaaac caaaagctaa aaatctaatg
    59821 gcaaataata gccaacgctt gagtgtttgc tctctttttg ttttttctgc taatgcttct
    59881 aatatcttaa tatgtggaaa atatgcttta atagtttttc tgaaatcaaa taaatgaatg
    59941 attaaaggta ttagcaataa aagcaaaaac caaagccttc ctggatataa aaagatcata
    60001 aaatagtttt atacaaaaat aaaaattatt attttatcaa aaggtaacct gagtattttt
    60061 tacgtgatca tatttttgcc aaaatctgat ctgatcattc ctttttttat taatgacaac
    60121 acaccttgtg cttgtatcaa atttaatgat ttgatatcta aattaagatc ttgacatgct
    60181 ttttctatca atttattatt ttcagcatca tatttagatc cttcatttat tatatgttcc
    60241 attagattca ttaagccata tctctctgca taccatattc tcaatggcag aatgcaatta
    60301 gctaaaatga tttttttatt ttctattgtt aaaattttat ttaatttttt aaaccaaaaa
    60361 tcacagccat ttataattaa cttatgaaaa aaatcagctt cattaactaa ataataaaag
    60421 cactcgacag ctttaggtat acgtatgatg gggtatgcat taggcctaat tcctttatgc
    60481 atccagatat ctttagacaa aggcaaaagt tgatacttct ttgatatcat ttcgatacta
    60541 ggatttatct cattataaag tccggaagca aacaaataag ctacttttat ttcatttaga
    60601 ttgtatctat cataaaatat cttgtatgaa atcttattag ctaacattgt cataggtata
    60661 gcattatctt tgagccccat agctctaaat aagcttatat acaaggtaaa tattaaatca
    60721 ttactagcaa aatcattaat taattgattc ccgatttgac gtagtctaca tataccttct
    60781 agcaataaat catcatacga aaattgatag ttttgtttat tattattatc attcaaaata
    60841 atttcaggtg atacttgtat tctaaatggt atctctaata taggcttaga tgcaggaaag
    60901 caaaaaacat gaagtataac attagtaaaa tgaggatttt ctatgtgacg atgtcttcta
    60961 taatcttcct cacaaatatg tatctctgta tttccaatca atttgatacc atctacttct
    61021 atagtagtac ctaaaaaatc cggtccatca tgcaaattat attttccagg ttctattacc
    61081 ttgattaatt tatcatctat agtagtaaat gcttgcatat cattactaat ccaccactta
    61141 tgaataatta attctttcat ttaatttcta attttgtata aaattaatat tttttatgaa
    61201 atctttgaaa atttctccat catatctaga attacatcta atggttttgc tatttggtat
    61261 tactggggtt ttaggtaaat taatttctct atcagcacta tcattagttt tttatagaat
    61321 gttaatagcc gctttatctt tattgttttg gttaaaaaga aaaaatttaa aactaaatct
    61381 atctaaaaaa gtattttgta tttctatatt tactggtatt ataatggcat tacattggta
    61441 tttatttttt tatgctatta aaatatccac tgtatcatta gtattaggat tattaggcac
    61501 tggcacttta ttcacttctt ttttagagcc tttgatatta aagaaaaaat tttcttattt
    61561 agattttatt tttgctattc ctatattgct cggagtacta ttgatatatt ggggtgagcc
    61621 agtacaatgg aagggtttag tgatagctat tatctcatat ttacttttta gcttattcag
    61681 tgtgataaat aaaaaaatag gcatagacta cgatgcagta gtgataagtt tttgggaatt
    61741 tctcagtggt agcatcttta tgttgatttt gactctagca aatgaaaaat taactttgcc
    61801 aaatataaca gatagtattt atttattaat actaggtgta gcctgcacag cttatgcttt
    61861 ttcagcattg attaggttaa tgcaaaaata cagtgcatat ttagtagtga tgcatgtaaa
    61921 tcttgaacca gtttatgcta ttttactttc attattaata ttcggtgaat cagagcaatt
    61981 gagtcttttg ttctacttag gtgccagctt aataatatta actttatttc tttatcaatt
    62041 aaaaggaaaa caagaaattt aatatttatg attctattca gaaataatgt tgttatacaa
    62101 tttttcatta tatatgataa atttattaaa cttttttttg tgaacttagt ggttaaataa
    62161 tattagaaac cacaaagcac acaaagatat tacgcaaaga acgcaaagat tattattcat
    62221 aattccgata tacctatcgg aaaacaacat tttctttaag tagagccaaa tttatttaat
    62281 gaacatatta attt
//
LOCUS       DAOPRN010000003        62169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_353_length_62169_cov_110.163393, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000003 DAOPRN010000000
VERSION     DAOPRN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 62169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_353_length_62169_cov_110.163393"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(463..2283)
                     /locus_tag="PKI65_00620"
     CDS             complement(463..2283)
                     /locus_tag="PKI65_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOB76979.1"
                     /translation="MKTYFQYPFLIGTIFLFAALIYIYIKWYVKLSKYDKVRFKKFWT
                     TNKVWKAIKDSINDGLLHLSIFKKNPVLGYMHMSLAFGWFLLIVIGHLEVVVAKGGLN
                     FPFYYAIFFRYFETVHATTIAKIFAAVMDFLLLFILSGVVLAITKRFYKKLFGMKRTT
                     KLKTFDKMALYSLWSIFPLRLLAESCSAAVYNNGSFLTNTTGAWLGNMINVSAIYEPS
                     WWLYSFALCFFFIALPFSRYMHIPTEIYYIFLRNAGIKTKKTWESYAQIQLYSCSRCG
                     ICLDACQLFDAGISDVQSVYFLQFIRNKVDAEKPLFGCLICGRCQVECPVHLNLNDLR
                     TALRIKASKPLTFSYAYTKKPEQQTADTIYFAGCMTHLTPNIKKSMTKIFDYVGENVW
                     FMDDDKSICCGRPLMLAGQYDSASQLISKNTELIIASGAKNIILSCPYCYKVFKEDYL
                     LPDINIKFHANYIKELIKQNKLPEGKLNLTAIYHDPCELGRGSNIYQEPREVIEHYAH
                     RIPLQKNKDKDRALCCGAGLGGVNLPAEAKNTVAYNTMSYFAKFNPDVLITACPLCKK
                     TFVRDRRIETMDISELVVKAIEQPNDSKSKETKEISYAII"
     gene            complement(2280..2624)
                     /locus_tag="PKI65_00625"
     CDS             complement(2280..2624)
                     /locus_tag="PKI65_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOB76980.1"
                     /translation="MGDLDFLKVKHLHIDYDKLELDLLNKVLEEEPSFQWCISCGCCT
                     ASCSAGNHTTFNVRRISLLLRRGEIESLEKELEKCMLCGKCTLVCPRDIQLRNVVMNI
                     KRNILKYKMILL"
     gene            complement(2825..3883)
                     /locus_tag="PKI65_00630"
     CDS             complement(2825..3883)
                     /locus_tag="PKI65_00630"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOB76981.1"
                     /translation="MYKKIKSKVKNINMKKIVVIGAGIAGLEVSKQLLQANLPVTLIE
                     QSSKIGGNIKNYAYLFPDFYNAEDLLNSYQYIITDPNLQLLLNAHVSKIDFSKKIYNV
                     ITKDGITIPADAIVIATGYNTFDARLKEEFGYGIYDNVITSVELEQMFKKQEIITPSG
                     KVPERIGFIHCVGSRDAKVSNTYCSRLCCITAIKQAIETKKLLPLVEIFCFYIDLRMY
                     GLLFEDLYLSAQRDYNIQFVRGRLSEASEMIDKKIQIKAEDTLIGRPLQMSADMIVLM
                     VGMEASDFSKQFKYHNDLNVNEHGFFDPINPHNLRNLTAKEGVFLAGTSIGPMSVGET
                     IDHARGTALSVLSYIKSN"
     gene            complement(3868..4917)
                     /locus_tag="PKI65_00635"
     CDS             complement(3868..4917)
                     /locus_tag="PKI65_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOB76982.1"
                     /translation="MDANIKVSINRIYWSEYIKEIPRDNYFFFRSCIRQTFFPGAEQM
                     FLNILSKNLNLNYKDDPDLTTCTGIGYHSDIIPLETVHTMVARHFSLMNESGYSNFVI
                     SCVTTFGVMTEALNMWHEFPETLEKTIENLYKTTKRTFELPKTIAHASDIIYLYRDEI
                     AKQCIYKLINKKTGQPLKGVDHVGCHYAKIFPQHGVGGAEFPKVLSEMIKSWGGEVID
                     YPARRHCCGFGFRQYFIKETRGYSLTHSQVKFESMEPYQPDFIITNCPGCNMFMDRWQ
                     YVIQETTGKVYSVDGNGIPVLTYEEVAAILLGYDPIEIGLFMHQTDVLPLLEKIGVEF
                     DKDRYLSLRSQCIKK"
     gene            complement(4920..5555)
                     /locus_tag="PKI65_00640"
     CDS             complement(4920..5555)
                     /locus_tag="PKI65_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOB76983.1"
                     /translation="MIPIEEIYSDPRIKENLNACINCGVCTAICPASDYYDYEPRYVI
                     KILLSKDENELEQLLSSDYIWYCGQCMSCKARCPRENAPGVAISALRYWSQKLGLFVK
                     SKMGRNQIIIKQTIGQNIYDLGYCVHPSKVTREAHPEQGPVWEWIFNNREKAYSLVNV
                     NIDREGPGPVRKISDKVMNEIHSILNGTGAVDFWDQIETSVNEYKNYHKNE"
     gene            complement(6033..6722)
                     /locus_tag="PKI65_00645"
     CDS             complement(6033..6722)
                     /locus_tag="PKI65_00645"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOB76984.1"
                     /translation="MGDHKTSVKRNVLILILIILFCDQVLKIYIKTNFLLGEKVCLIG
                     NWFCLYFVENAGMAYGIQWGGSIGKYILTIFRIVAAAVILWFLIKSFKKNHHSLFYYS
                     IALIFAGAIGNIIDSMFYGLIFSESGIYGFDTQPARFVPIGQGYAPFMQGRVVDMLYF
                     PIINTTLPDWFPIWGGKNFVFFQPIFNIADASITTGVLIMLIFYKKVFSYSKDDSPLD
                     VTTDMENEFNN"
     gene            complement(6825..7259)
                     /locus_tag="PKI65_00650"
     CDS             complement(6825..7259)
                     /locus_tag="PKI65_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOB76985.1"
                     /translation="MATNNNFDEKTRYSDEELEEFKQIILKKLEKAKSDLQILRESMS
                     SDGEIETNLTFRLQEDSKENLMREENAKLIARLERFVRDLELALVRIENKTYGICRVT
                     GKLIDKERLKAVPHATLSIEAKLQMQRGEIPINKENEEEIEG"
     gene            complement(7281..10637)
                     /gene="ileS"
                     /locus_tag="PKI65_00655"
     CDS             complement(7281..10637)
                     /gene="ileS"
                     /locus_tag="PKI65_00655"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOB76986.1"
                     /translation="MRKYREYKSLNLPNIANEVLDFWETNDLFKKSIKNREGQPCFVF
                     YEGPPSANGMPGIHHVMSRTIKDLFCRYKTLKGYQVNRKAGWDTHGLPVEIAVESKLG
                     ISKEDIGKKISIADFNNQCRKEVMKYKDKWEELTKIMGYWIDLDHPYITFDNKYIETL
                     WYLLKQLYNNGLLYKGYTIQPYSPAAGTGLSSHELNQPGCYKIIKDYSVVAQFKVIKN
                     EKSEFLFNGAEGDVYFLAWTTTPWTLPSNTALAVGEDIEYAEVITINRYTKEKQTVIL
                     ARNVIDRWFSPNNENLPFESFDPTKNIIPYKILRTFRGLDLLNVRYEQLLPYAKPDRG
                     DPFRVIIGDFVSTEEGTGIVHIAPSFGADDFRMDKLYDIGALTLVDKQGKFTKEMGEF
                     AGKYVKAEYYTDHSIEKEKIVDLEIIAKLKRENKAFATEKYEHSYPHCWRTDKPIIYY
                     PLDSWFIKTTAYKERMIELNRTINWKPEFTGTGRFGNWLENMVDWNLSRTRFWGTPLP
                     IWRTTDKTEEKCIGSVEELIKEIEKSIECGYMKSNPFKDFVIGDDSEENYKKIDLHRP
                     YVDDIILVSPSGKPMYREPDVIDVWFDSGAMPYAQLHFPFENIDVFEKNFPADFIAEG
                     VDQTRGWFYTLHAIATMIFDSVAFKNVIANGLVLDKNGFKMSKRLGNVVDPFEIINHY
                     SADALRFYIITNSQPWDSLRFDIECVKEVIRRFFGTLYNTYSFFALYANIDGFTYCEP
                     EIPFEERLELDKWILSELNSLIKNVDEAYEDYEPTRAGRLIEQFTIDHLSNWYVRLSR
                     RRFWKGDYSIDKISAYQTLYTCLETIAILLSPIAPFYSERLFRDLNEVTIKKSHESVH
                     LLDFPIYNSNYIDKELEDRMELAQKISSMILSLRKKANIRVRQPLSKAIIPADENTIT
                     QLEKVKHLILHETNIKDLEYISLQNDFLVKKAKPNYKLLGPKYGRLMKEISAIIQDWD
                     NNMINEIETNKKITITTSEGHIEILLEEVEIFTEDIPGWMVAHDNNLTVALDITITEK
                     LRMEGVARDFVNRIQNLRKDKNYDVTDKIRVFFYSSDPFVDKSIFENYSYICNEILAK
                     EIKNISINGLEADVITVDDNIDVLVHIEKI"
     gene            10714..11334
                     /locus_tag="PKI65_00660"
     CDS             10714..11334
                     /locus_tag="PKI65_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOB76987.1"
                     /translation="MSKNKYYVVWIGRKPGIYTSWAECKAQVESYPGAKYMGFTTYDQ
                     ALLAYQAPVPSPDVVNPILEQIQYLFPPDSIAVDASVNMKTGTMEYRGIHLSSMQLIF
                     HVGPWDYGLKSNNIAEFLALVHGLQWLMQYDLDAPVYSDSITAMAWVRNKKINSKTLK
                     KMPIPQIIEKVDNAIAWLHDNPNHAEVRKWNTEQWGEIPADFNRKS"
     gene            11378..11449
                     /locus_tag="PKI65_00665"
     tRNA            11378..11449
                     /locus_tag="PKI65_00665"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11411..11413,aa:Glu,seq:ctc)
     gene            11580..12257
                     /gene="clpP"
                     /locus_tag="PKI65_00670"
     CDS             11580..12257
                     /gene="clpP"
                     /locus_tag="PKI65_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOB76988.1"
                     /translation="MKRKNEFYSYATKHIGISSQTLDDYIIASNGYISPTIIEERQLN
                     VAQMDVFSRLMMDRIIFLGVPIDDYVSNIVTAQLLFLESTDPQKDVFLYMNTPGGGVY
                     AGLGIYDTMQYVAPDIATICTGIAASMGAVLLCAGTKGKRSALPHSRIMIHQPMGGAE
                     GQATDIEIIAKEIKKLKDELYQIISYHTGQPFEKVEKDGDRDFWMRSDEAKEYGMIDE
                     VLTKNKE"
     gene            complement(12478..13923)
                     /locus_tag="PKI65_00675"
     CDS             complement(12478..13923)
                     /locus_tag="PKI65_00675"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOB76989.1"
                     /translation="MFELIFSNIHSTTKNLVWVGTKIDQLPSEIDDKDKNIINKELNR
                     SNNVVVFNRSTHLEIIVILEPSDKEEIRKVGSKVSKIVQDENLSELILQCNDEVLIYF
                     YEFVEGLFLNSYRFNQYKSLKTENEVKYLLQVSDKYEAYFIELYHLVKSVFWARDLVN
                     RAPSDLYSSIFVQEILNLFEGIAINIEILEKKQIESMKMGGLLGVNKGSSTPPYFVIL
                     HHNDDQALNTKPFIFVGKGITFDTGGISLKPAKNMHEMKSDMAGGAVVASLMYALAIN
                     HIPVNAIGLIPITDNMPGPNSLLPGDIITMMDGRTVEVVNTDAEGRLILADALCLAQK
                     YAPTLVISIATLTGAAHKALGHYVIAGMQSKASAFFERLEKIGMDTYERIVEFPLWEE
                     YFNPIKSDIADIKNAGNEYAGAITAGKFLEFFSDFPFIHLDIAGPAYITKHWGYWPQG
                     GTGVGVRLLYNYLKSLLDLPEFNEIITQNEN"
     gene            complement(14036..14779)
                     /locus_tag="PKI65_00680"
     CDS             complement(14036..14779)
                     /locus_tag="PKI65_00680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOB76990.1"
                     /translation="MKTKTPSLLFIFIFLIYSCSSTKEISKTTNDNEINQETKSTISF
                     NNFRDTISCLIGNDLARNFINNGLDIDINLLKAGYEDVLNNADTLFTNEEFQAAMQRL
                     QREAMQKAEAKRLEEANLNKEVGKKFLENNKNNPDVYETTSGLQYKVIKMGEGPKPTI
                     ADKVRVHYEGKLLNGKVFDSSYQRGEPIEFQLNQVIPGWAEGLTLMPVGSIFELYIPS
                     ELGYGDRPIGEIPAGSTIIFKVELLEIVE"
     gene            complement(15075..17195)
                     /locus_tag="PKI65_00685"
     CDS             complement(15075..17195)
                     /locus_tag="PKI65_00685"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOB76991.1"
                     /translation="MKKIYNFIRILLFLSIIMFSLPVWSQLNVDVSYSAFDLVQNILI
                     GQGITVTNVQFTGNNAQKAYFYGTTNIGFTDGVLLTTGGATVAIGPNNSGSASVSVDT
                     PGDADLSAIIGVPTNDASVLEFDFIPQADTISFYYVFASEEYPEFVCSSFNDVFAFLL
                     TGPDPSGGMYNKKNIAIIPNTNLPVAINTVNPGYPGSGYSASGCTSLAYSQYYVDNTG
                     GQTIQFDGFTVPLKAFAHVVPCETYHIKLAIADATDHSYDSGVFLQAGSFSANSYDIQ
                     MENINISESIPDNSIIEGLNCGSYTQICFTRSFDINTNDTLTIFLDGTAINGVDYIDE
                     NGNPIPTQFIFTPDTYEQCINIYAIADTLDESTETIIIKVPSLNPCIGSDTIRDTVYI
                     INHMPFEVYITGDTIICEGESGQLIANTTQFPAFFHWSTGSQMGSINIHPTQEITYYS
                     IIGTDEYMCIDYDTIMVRLLPKAEVNLGNDTCITSGPFALDATQQNSVFSYLWSTGHT
                     TSMIDVLESGIYWVSVTNSTYDMCDDADTINVKIIPTPIVDLPQNILLCSHEFITLSV
                     SQFNNDLYEFIWSDGSNDTNYILDKPLPGIYDINVKVIGCDTISDSTTIIVEDCEINI
                     PNVITPNGDGINDAFYITNIEFYPNSEIKIYNRWGKRVYESQNYQGDWDGDRLDEGTY
                     YYIFRLNYGRGKIEEIHGTITIIR"
     gene            complement(17269..17682)
                     /locus_tag="PKI65_00690"
     CDS             complement(17269..17682)
                     /locus_tag="PKI65_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB76992.1"
                     /translation="MKGNLLSLIILLTFISCQSKADKVKELKLDAITHIYKRDDETAK
                     QKLNKAVKLTPNDPEIYYLMGNILFNESNYQEAINYYEKTIELDSTYAQAYTSLGKIY
                     RIFNDRDKWCENFVKAYQLGDKTVYNDVRHCLPGI"
     gene            complement(17700..19043)
                     /locus_tag="PKI65_00695"
     CDS             complement(17700..19043)
                     /locus_tag="PKI65_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOB76993.1"
                     /translation="MGAFHAYDIRGIYGKDFNNEDVLKIGYYFPQIFNVKKILIGRDV
                     RKSSDAIRDTLIEGLMCSGCDIYDAGLATTPSIYWATAKYHFDGSIMITASHNPKEYN
                     GLKFSGKNAVPIGYDDGLNKIEKLIKENKKIEIVKNGTYKEFDFKIEYLNFLKKYLKP
                     DLDLNFTIDCSNGMAGLFIHQIFDEGPYYIFDELDGNFPNHEANPLDEKNLEALKQNV
                     AKRKDDFGMIFDGDADRVMFVDENSNFISPDLMIALLGHYFLEERGEKGLVLQDIRTS
                     RSVAEYVEPMGGKIVMWRVGRAYAANKLREIDGVYGGELAGHYYFRDFYYSDSGLLAS
                     ILITNIISDFVKKGYKVSEIIARIKKYENSGEINYKINRKLDAMNAVKDYFFNEQKPI
                     AFYDFDGYRLDYVDWWFNIRPSNTEPYLRLIVEAENKGLLENKLNIIDKIISGYD"
     gene            19294..>19595
                     /locus_tag="PKI65_00700"
     CDS             19294..>19595
                     /locus_tag="PKI65_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB76994.1"
                     /translation="MRQLNICIFLSFIHLTLFSQISFTDLDRLTRITKDVKALSHDTM
                     MGRKSATKYEWKAGNYIISELNKISVQKLPGYESFRLAFTINNDKIKRDTTADIIA"
     assembly_gap    19596..19695
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19696..20394
                     /locus_tag="PKI65_00705"
     CDS             <19696..20394
                     /locus_tag="PKI65_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOB76995.1"
                     /translation="TADIIAYIDNGAPYTIILGAHYDHIGYEVYDITKNQYLIYNGAD
                     DNASGVACLLELARHLSTGFLPQHNYIIAFWGSEEIGLYGSTYFCQKILINLKVPPII
                     LYINYDMVGSLGYKKNELILYGSATGKNIKKYIPRKYDTVKIIKLPVSFSFSDHTCFY
                     KNKIPYLYFTTGLPKVYHTIKDEFRLINFNGILFTLKLTEYILETIEIESIRYNKACS
                     LNYIITGVKLLMSK"
     gene            20469..20738
                     /locus_tag="PKI65_00710"
     CDS             20469..20738
                     /locus_tag="PKI65_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOB76996.1"
                     /translation="MKIKPLADRVVIEPAPAEEKTAGGIIIPDTAKEKPLKGTVVAVG
                     PGKKDEPMTVKAGDVVLYSKYAGTEITIDGKEYLIVKESDILAII"
     gene            20766..22385
                     /gene="groL"
                     /locus_tag="PKI65_00715"
     CDS             20766..22385
                     /gene="groL"
                     /locus_tag="PKI65_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573313.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOB76997.1"
                     /translation="MAKEIILGYEAREQLKKGVDKLANAVKITLGPRGRNVVLERSYG
                     APVSTKDGVSVAKEISLEDKFENMGAQMVKKAASEASDAAGDGTTTATLLTQIIYNAG
                     LKSVTAGANPMDLKRGIDKAVKAVIEHIKQQSQQIGDSYEKIEQVATISANNDREIGK
                     TIAEAMRKVKKEGVITIEEAKGTETTVDIVEGMQFDRGYISPYFVTNSEKMIVEYENP
                     YILLYENKLSGMKELLPILEKVFQTNRPLIIIAEDVEGEALATLVVNKLRGSLKVAAV
                     KAPAFGDRRKEMLEDIATLTGGTVISEEKGFKLEDATLDLLGQAKKVTIDKDNTTIVE
                     GAGSKEAIEARIKQIKVQIEDATSDYDKEKLQERLAKLAGGVAVIRVGAATEVELKEK
                     KDRYDDALHATRAAVEEGIIPGGGVAYIRAIEALDNIDVFNEDEKIGVNIIRRALEEP
                     LRQILENAGLEGSVIVQKVKEGKNDFGFNAYTEKFENLLETGVIDPTKVSRVALEKAA
                     SIAGSLLITEAAVAEIPEETPAAPAMPPMDY"
     gene            22756..23256
                     /locus_tag="PKI65_00720"
     CDS             22756..23256
                     /locus_tag="PKI65_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_022071788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(P)"
                     /protein_id="HOB76998.1"
                     /translation="MVSKTDAIEIITYAEENGINIWIDGGWGVDALLEEETRVHNDID
                     LFVEERNSKKFIEVIKEKGFAEVTEAYTTTSHTVWKDNKGRIIDLHIFKFNEQGYIVF
                     EGEAYPPKVFSGIGKIGDKMVRCIDAENQVLFHLGYEHDENDIHDVKLLCQRFDIPVP
                     SEYKSV"
     gene            23718..24194
                     /locus_tag="PKI65_00725"
     CDS             23718..24194
                     /locus_tag="PKI65_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOB76999.1"
                     /translation="MKQKPKKVAVLAATDRAGNLLFEKFEGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEIVTSHRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNITYVN"
     gene            24469..26382
                     /locus_tag="PKI65_00730"
     CDS             24469..26382
                     /locus_tag="PKI65_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOB77000.1"
                     /translation="MSEYLPHECGIVLLRLLQPLSYYMEKYNCCYHGLDMLYLLMEKQ
                     HNRGQDGAGVVSVKLDVEPGYRYLDRIRSNVDSPIIDIFQKIYDPLRILEANNSPLLY
                     DPEYLKRNIPFISELYLGHLRYGTYGKYQIEYVHPHLRQSNWRTRTLALVGNFNLTNA
                     DQLFLDLINYGQHPIETSDTVTILEKLGYYLDEMNNELYEKFKTQGYTKAEISPKIAE
                     TLDIVSIVKKAAQNFDGGYVIAGVLGHGDAFVFRDPRGIRPAYYYKDDEFFVVTSERP
                     PIQTAFNVKTDDIKEMPPGHVLILKKNKDLSIVPYTDNIPSKMACSFEHIYFSRGTDR
                     DIYKERKMLGAMLADPILSAIDYDLENTVFTYIPNTSTVAFLGLYAELEKWLDVQKKE
                     KIIDIINMGENINDKELRAILNWHPRADTLVVKDLKHRTFITKESKRNGLASHVYDVT
                     YGVVRPNIDRIVAIDDSIVRGTTIKKSIIPMLNRLEPKEIVIASTAPQIRYPDCYGID
                     MARLEEFIAFQAAISLLKERGQAKLIKYVYDKCKEQDSLPDNKLENYVSLIYQPFEEE
                     EITHKIEELLTPIANKTKLTIIYQSLEGLSKVLQDNKGDWYFTGNFPTIGGYRVVNNA
                     FINYYEGKNKRAY"
     gene            complement(26516..27874)
                     /locus_tag="PKI65_00735"
     CDS             complement(26516..27874)
                     /locus_tag="PKI65_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOB77001.1"
                     /translation="MKIHIIAIGGAVMHQLAIALQQAGHIVSGSDDEIFNPAKENLEK
                     YNLLPCEIGFFADRITPDIDLIISGMHAKANNPELTEAKKLNLDVLSMPEFIYQHSKN
                     KHRIVIGGSHGKTTVTGMIMHVLRLLNKQFDYLVGSKVVGFDFNIKLSNEAPIMIIEG
                     DEYLASTELPIPKFIIYKPDVGLINGIDWDHANVFPSFENYLEQFKKFAEMVPSDGAL
                     FAVDEPIVRKILDEAQLKLTPIFYKTFPYFVENGHFFLKYQEKSYPLQIFGKHNMQNI
                     SAAHKICEYLDIDTKDFCEAMMSFRSANKRLEKLYENKNLVVFRDFAHAPSKVRATID
                     AVREHYPDRQLISVFELHTYSSLNQNFLPQYSKTMQNSDISIVFYSKHALEIKQLPDL
                     NTIDIIKAFDQQNLFVTTIVNDIQNIIEQYIKLPAAIVLMSSGNFDNWELNINTIKEI
                     VN"
     gene            28176..29672
                     /gene="rpoN"
                     /locus_tag="PKI65_00740"
     CDS             28176..29672
                     /gene="rpoN"
                     /locus_tag="PKI65_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOB77002.1"
                     /translation="MLKQTQHLRLLQRLSPQQIQLMKLIQLPIAELEQRIKEELISNP
                     ALDTLDDTDDDENIMIIDDEIDKNNELDYPDIDDEKTDIINDDDELLDEYIDEDEIPE
                     YKLYINNTSPDDSTNIQYYDYTTKTLNDYLKEQLDYKNLSEQEYLIGEYIIGNIDDNG
                     YLQRSIDAIVNDLLFIRGINTNADEVEKVLDIIQRFDPPGIGARDLKECLLLQLEAKE
                     HKTPEINLAIEILENYYDDFIKKHYDQIIKKANITEDQLKMAISEIQKLNPKPGNSLY
                     QIGNSAISIIPDFIIYNNDGELELTLNNYNIPYLCTNKKYENMYQSLSKEASNDPKKK
                     ETVQFIKQKLDGARWFINALEQRQNTLYITMKTIMELQKDFFMTGDETKLKPMILKDI
                     ADKINMDISTVSRVANSKYVATPYGTFLLKHFFSDSLETKSGKEVSNKTIKAIIKDII
                     KNEDKSNPLTDEELTKILEEKGFKIARRTVAKYRDQLNIPVARLRKEL"
     gene            29690..30301
                     /locus_tag="PKI65_00745"
     CDS             29690..30301
                     /locus_tag="PKI65_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77003.1"
                     /translation="MKKNLSQVISIALHPVFIPIWFAIILFNSGYFLNAFLPNAFFKS
                     YIWLLIIIMVIIPTIIILFSYHLGLIESVHSSIPIERIKILLIILVSSFILYFFLKRL
                     HIPIFYLLPIRLTIILTTLLCIFSSFMNVSIHSAGWMNLFTSLYILQYRLIEINIFWI
                     IVIIPILWGLAAQARYVVGKHNRLQIFMGAFLGIITGLSVFMV"
     gene            30465..30611
                     /locus_tag="PKI65_00750"
     CDS             30465..30611
                     /locus_tag="PKI65_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77004.1"
                     /translation="MKKYQDKMNTHTETKKSKNIKIKMPKGYKKSKVDISDIEEIKYK
                     ENKK"
     gene            30608..31942
                     /locus_tag="PKI65_00755"
     CDS             30608..31942
                     /locus_tag="PKI65_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77005.1"
                     /translation="MNILIVAYYWPPAGGSGVQRWLYMSNHLADIPGNNLTIYVPDTT
                     DYQVLDPTLSADISPKLKIIKRPIWDPAHFYNKAVGEKNKIGVGMSSVKEKKSLIQKI
                     GLWFRANVFVPDSRVFWVNPSYRFLKKYIEKNKIDVIVTTGPPHSMHLIGLKLKQYFK
                     EKIIWVADFRDPWSEIQYYETLKTTKFAFNKIKKLEKKVLINSDIVTTVTPSWANMFR
                     DISKRKIEIIENGYEDSLFEHIKIINQPKRFNIVHTGVMYATRNPHNLWNSLRIIKNE
                     IPAFVDLAKIQLIGNLDASVINELKNLDLYDITEIYPPMSHKEVIEYQINAALLLLII
                     DQAKTYISTAKGMITGKIYEYFACQRPILGIGPTDGDAARILGDSEYAKMVDWDDLEG
                     IKNFITNIYQKYINGDKLVVDYHQKELYSRKNLALKFNNILLEYINNKKNNG"
     gene            complement(32127..32708)
                     /locus_tag="PKI65_00760"
     CDS             complement(32127..32708)
                     /locus_tag="PKI65_00760"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77006.1"
                     /translation="MCKIFLYTFIIFLIFFKLQGQNEGIYKDLNTALSDAEKVKVLIL
                     KNKKLEKLTSKIDTFQNLEVLVLKRNKLNSLPENIINCKKIIKIDLSYNKFKDFPIIL
                     IELPNLKELNLYHNEIEYLPNEIKEFCCLEKLDLGDNYLTKLNQSITGLNNTLKVLNL
                     RLNMIPLKHQKEVRERYLPNTKIYFSTPCNCDL"
     gene            complement(32849..33580)
                     /locus_tag="PKI65_00765"
     CDS             complement(32849..33580)
                     /locus_tag="PKI65_00765"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOB77007.1"
                     /translation="MERKIIILIALIILFFSCEKQESCLMSVGKYSEIIIPLDSIDRL
                     YVEGRVNLYLIKDSIDFLKIKGGENLINGVSINQKNYELKIKDKNKCYWLRNYKHWID
                     IETHYRNINYINPEIYGEMIFENTCIQDTINIEVWDSALDIKGELNVNRFIFRSHGGN
                     VGLYITGQSKYLYTYLHGAGPQHLEYLCSDTVFVNSCSYQNIYVCVNNVLWVDINSNG
                     NVYFRGNPSEKNFNYKGSGKVLPLE"
     gene            complement(33565..34605)
                     /locus_tag="PKI65_00770"
     CDS             complement(33565..34605)
                     /locus_tag="PKI65_00770"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOB77008.1"
                     /translation="MKKIILFIYFIPILAFSQLSIGISGSYGFLFPHYKKMYNFIENH
                     PAGFSLFYENKYYPIDTFQSPKRYRIEAYYTSLSNNKVLGQAYSLLGDIYFRVYKEKI
                     YFFLGSGVSYLSKHYTEDNYRNFAIGSHFNVHINFGWDFIIYQKSPLLINMQFRWNHF
                     SNGAIKMPNTGLNIPSLHLSIGYFQEKHNKNKKIDTFNLFQPKYHSFGTYSIKQNRTN
                     DVYYSVYTTSFEYYVSHYNAVQWMVGTDFIWDGSLPDYLKTKDFDLDKYNAIPLKISL
                     KGGWDVHIDKMDLIFQVGIYLRNLAFKYQPFYTRFGIRYFFSNNFGAQISLRSHYVKA
                     DVIEWGLVWRGK"
     gene            34838..38116
                     /locus_tag="PKI65_00775"
     CDS             34838..38116
                     /locus_tag="PKI65_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOB77009.1"
                     /translation="MKRLSIFLLLNLILIKMSYSQQETRLLRFPTTDGSKIVFTYAGQ
                     LYTVSKDGGIARELTTGDGYAIFPRFSPDGKWIAFTGQYDGNTEVYLMPAEGGIPKRL
                     TYTATLNRDDLSDRMGPNNIVIGWTPDSKYIIYRSRKSSFNDFVGQLFIVSIDGGLST
                     QLPLSSGGFCSYSPDSKYLAFNRVFREFRTWKYYRGGMADDIRIFDFDSKNIINITNN
                     EAQDIIPMWIGEEIYFISDRDRIMNLFVYNTKTEETKKVTNFDDYDIKFPSHSVTNGL
                     IVFEKGGYIYLFDTKTKEANKVSIIINQDLPLARTLKVNAADYITSFTLSPGGERMGF
                     SARGDIFSVPATEGVVRNLAKSSGVHERNVEWSPDGKYLAYLADNTGEFEIYIQKHDG
                     SEPPIQLTKNADTYYFEILWSPDSKKLLFSDKKLRLRYVDIDTKKITEVAQSQVWEYR
                     DFTWSPDSRYIAYSDPQKNDFNIIRIYDTYTKETFDLTDMWYDSHSPHFSPDGKYLYF
                     VSERDFNPIYSRVEWNYAYQDISKVYLAILDKSIPNPLAPRNDDLDLSKKTADDKKSD
                     KKKKDKEITEEDALVKVVIDIDGLKDRIISLPIEVSTYWNLQATNDKILYQRRGIKDE
                     KTQLLAFDLKERKETKLGEFDTYLLSNDQKKILIKSKNNYYVLDFAVAELKPDKKVPT
                     QEMVVWVDKKAEWEQIFYEAWRQMRDFFYVPNMHGVDWEKIKNKYEVLLPYVANKHDL
                     NYIIGEMVGELSVGHAYIGGGDLPTINKIYTGLLGAELSKDTQSGYFKIDKILEGANW
                     SRDLRSPLQELQVNAKVDDYIISIDNIQTNTVNDIYKLLIDKANKQVEIVINDKPNNE
                     GARKVIVVPVANEATLYYYNWVQNNIKLVNEKTNGQVGYIHIPDMGVDGLNEFVKYFY
                     PQLTKKALIIDDRGNGGGNVSPMILERLLRVPQRATMARNVLIPGVVPTQTMVGPKVL
                     LFNQYSASDGDLFPYGFKKYNIGKTIGVRSWGGVIGIRGSLPFVDGTYLNKPEFASYS
                     IDESKWIIEGYGVEPDIVIDNDPYREYLGIDDQLLKAIEVIMEELKNAKEIPDIPTPP
                     DKSK"
     gene            complement(38296..39150)
                     /gene="ispE"
                     /locus_tag="PKI65_00780"
     CDS             complement(38296..39150)
                     /gene="ispE"
                     /locus_tag="PKI65_00780"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOB77010.1"
                     /translation="MKDKIIIEKAPAKINLGLFVLKKRDNGYHDISTIMYPINDFYDI
                     ITISISETLSLTVPNHIELENEDNLVFKAYNLLKNKFNLPPVKIVLEKNIPYQAGLGG
                     GSSDAVATLKALNKLFNLGLDANELLKFSIKLGSDCPFFVYANPLIAEKQGDILTTIN
                     LSLENKYIYIFLPYLNDKPIQVSTTEAYSQIKPNEFREDIKTIIKNYPMVQWKYQLKN
                     DFLEIFINKYPFLYELIEKLYELGAIYVNLSGSGSAIFAIFDNKNKKLEYSSIINKYC
                     KVIYKGYL"
     gene            complement(39158..40237)
                     /gene="fbaA"
                     /locus_tag="PKI65_00785"
     CDS             complement(39158..40237)
                     /gene="fbaA"
                     /locus_tag="PKI65_00785"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOB77011.1"
                     /translation="MSIKNQLKPGIVWGDALKALYEDAKKREYALPAVNVVGTNSVNA
                     VLEAARDVHSPVIIQFSSGGAQFFAGKGLSNENYEAAIIGAISGAMHIHNVAEYYGVP
                     VVIHTDHAQKKLLPWVDGMLKAGEKYFKEKGKPLFSSHMLDLSEETIEENISISKSFL
                     ERMHKIGMFLEIELGVTGGEEDGVYHSAIDNPKLYTQPDHVAYAYEELKNISPNFTIA
                     AAFGNVHGVYKPGAVVLKPVILKNSQEYICKKLGISEPKPVSFVFHGGSGSALEEIRE
                     AVSYGVVKMNIDTDTQWAFWDGVHKYYLKYKDYLQGQIGNPEGPDKPNKKYYDPRAWL
                     REGEISMRERVKIAFDDLKSLNILD"
     gene            40467..41480
                     /gene="gap"
                     /locus_tag="PKI65_00790"
     CDS             40467..41480
                     /gene="gap"
                     /locus_tag="PKI65_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOB77012.1"
                     /translation="MKTVRIAINGFGRIGRNAFKILESSKDVDIVAINDLTAPATLAH
                     LLKYDSIFGKFEGTVDTKEQSIFVNGKEYKIYAEKDPSVLPWKELDIDIVLECTGKFR
                     SRDGMMKHIEAGAKKVILSAPASKGEDVDITVVLGVNDDKITKDHILISNASCTTNCL
                     APVVKVLHDNFGIEYGFMNTIHAYTNDQIILDSPHKDLRRARAAAMSIIPTSTGAAKA
                     IGLVIPELAGKLNGSSMRVPVPDGSVVDFYALLKKDTSVKEINAIMKNYAEGSLKGIL
                     EYCEDPIVSSDIIGNPHSSILDAELTNISEKRFVRIVSWYDNEFGYSNRLAEIAIKVG
                     KIE"
     gene            41713..42336
                     /gene="folE"
                     /locus_tag="PKI65_00795"
     CDS             41713..42336
                     /gene="folE"
                     /locus_tag="PKI65_00795"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004366384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOB77013.1"
                     /translation="MLDDFEPGDGYSRIDYYDEETTNKLAEYYYEIIKLIGDDPNREG
                     LQRTPIRVAKAMQFLMHGYYADPEQILRSAMFKEDYREMVIVKDIEIYSMCEHHMLPF
                     FGKAHVAYIPYGNIVGLSKIARVVDVFARRLQVQERLTSQIKDCIQNVLHPMGVAVVI
                     EAHHLCMMMRGIQKQNSVMVTSDFTGAFRKEETRAEFLSLIGTKTLH"
     gene            42538..42609
                     /locus_tag="PKI65_00800"
     tRNA            42538..42609
                     /locus_tag="PKI65_00800"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42570..42572,aa:Met,seq:cat)
     gene            complement(42607..44190)
                     /locus_tag="PKI65_00805"
     CDS             complement(42607..44190)
                     /locus_tag="PKI65_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOB77014.1"
                     /translation="MKKFVFIFILLPFTLFAQNITKVNSNINEIKIFLNGAQITRISD
                     INLQKGDNKILISNLPDDLDPKTVNVKGQGDFIIQTVNVSINYLSDYAKDPTYKILKD
                     SLELLQQKLDKNNTILQVYIEEEAMIKANKTIGGTQNGTNVNELIKASEFFRSRLLDI
                     KTRSLAIQRDNKELLEAINKLQNQIASYEKIAKRPVGEILLNLYADKATNGRLEVSYF
                     TNYANWRPSYDLRFVDLNNPISLFLKGEIIQTTGENWENVKLILSTSNPTISQQKPKL
                     YPWYLSVYMPKATSDIEIRGQRENIAVAYAEEEIKTYNKPAPVMMAIGYETPISMEYT
                     LEAKIDVPSNQEKIVNIQSYDLPAKYLYYTAPKIDPDAFLIANIYDWDKYYFLDGIAN
                     IYCENSFVGTTKISSYQIEDTMQVSLGRDKNVIVTRNSIKDSTAKQLIGSKVKYIRAY
                     EIVVRNNKKLPIEIIIEDQLPISQQKEIEVTEIEVGNGKIDPDTKIVTWRYDILPNQT
                     VKTKLKFVVKSPKELNLAL"
     gene            44649..45491
                     /locus_tag="PKI65_00810"
     CDS             44649..45491
                     /locus_tag="PKI65_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOB77015.1"
                     /translation="MKNFLVGIDFSKGSIKALEYAVMLNKYIDSKITMLYVDKPIPTE
                     SIYATVTANYRSELHQRFEQLLNEFRQHIKEPENLEYKITTGKVYEELANYAKKNNVD
                     YIFAGTHGISGYEELWIGSNANRIVSTAPCPVFIVRQTYEVHDKINSILLPIDSTAET
                     TNKLPWAIEIAKNTGAEIHIFGAYITDLTTLKNKVNKNVAMIESKLETENINFKTFKK
                     NISNYASDLITYIEENNIDLAIIMTEQENRNTNILLGQYAMQMINHCPVPVLSIHQDD
                     KYNF"
     gene            45492..46892
                     /locus_tag="PKI65_00815"
     CDS             45492..46892
                     /locus_tag="PKI65_00815"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOB77016.1"
                     /translation="MNPLFLISKEATKLGRINSLKPISASGSVRFYAIVYFNDTALLG
                     CYNDNVKENEAFIYFTNFFNNYNLPVPKIVHVNKDKTIYFQTFISDQTIYDKLKYNNY
                     LFDSTIIDYYKQALKLLTNFNKLTNIDFSKTFPRHCFDSRCIRWDLNYFKYNFLKIIN
                     IPFDEDKLENDFDLLEKKILSFPQNYLSLRDYQSSNLLIKENKIYVIDYQGARLGSQM
                     YDVASIIFDSKARIPHEIKLELFDYYLDLISIDDNDKTKLKEEFYWLALFRLMQAFGT
                     FGYRGLIQNKPSFTSSIIYGSKNLEIIYKFLNFNDYCQLPIIIENIINKYQSNDNLIG
                     KKILNIKIYSFSIKKHYPPLDMEHGGGFIFDCRCIINPGIIPEMQSLNGKDQLVIQYL
                     KTSEIVQNFLNYTFNTVLLATEEYLAKGYTFMQIGFGCTGGQHRSVFCAEEIARRLKE
                     FFGEKINIYIKHLELE"
     gene            complement(47140..47460)
                     /locus_tag="PKI65_00820"
     CDS             complement(47140..47460)
                     /locus_tag="PKI65_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOB77017.1"
                     /translation="MNEKDKKDKKENQINVVLPPEIADGVYSNLAIISHSTDEFVLDF
                     LSIVPGVPDAKVKSRIICTPQHAKKLLRALSDNIQRYENSFGEIIEYEHKMPNINTKF
                     YGEA"
     gene            complement(47464..51741)
                     /gene="rpoC"
                     /locus_tag="PKI65_00825"
     CDS             complement(47464..51741)
                     /gene="rpoC"
                     /locus_tag="PKI65_00825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOB77018.1"
                     /translation="MAFKSDSIPTNFSSIIISIASPDDILAMSHGEVTKPETINYRTY
                     KPERDGLFCERIFGPVKDYECACGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQL
                     SVPIAHTWYFRSNPNKIGALLGLNSKQVNNIVYYEKYVVTNPGIMAKENINQLDFLTE
                     EEYFNILENIPIENQQLSDDNPDKFIAKMGGEALLDLLSKIDLDELSYQLRDKASKET
                     SQQRKSDLLKRLQVVEDFREAQTRIKNRPEWMILKVLPVIPPDLRPLVPLDGGRFASS
                     DLNELYRRVIIRNNRLKRLVDIKAPDVILRNEKRMLQEAVDSLFDNTRKVAVTGSSLN
                     RPLKSLSDSLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELRMNEVGLPKEMAAELFKP
                     FIIRHLLERGIVKTVKSAKKVIERKDPVIWEILENVLRGHPVLLNRAPTLHRLSIQAF
                     QPKMIEAKAIQLHPLVCTAFNADFDGDQMAVHVPLGNEAIAEAQLLMLSTHNILNPAN
                     GAPIAVPSQDMVLGLYYLTKARKSTNDFPVRGEGKIFYSTDEALIAYNDGKADIHAIV
                     KVMINDKDGNPTLIETTIGRVILNQIVPKEIGFINKLITKSIIRDIIADVLKATNNVV
                     TTQFLDDLKNLGFKMSFKGGLSFNLGDLIVPEEKIDIINEAKQEVEEITLNYNMGLIT
                     NNERYNQVIDTWTHANAKLTQILIKQIASDKQGFNPVFMMLDSGARGSKEQIRQLCGM
                     RGLMAKPQKSTTSSSEIIENPIIANFKEGLSMLEYFISSHGARKGLADTALKTADAGY
                     LTRRLVDVAQDVIVSEDDCGTLRGLTVGDLRNGDEIVETMYDRILGRVSLHDIQKPIT
                     GEIIVHEGEEITEEKAQAIVDAGITEVEIRSVLTCESRHGVCAKCYGRNLATGKLVNI
                     GEAVGVIAAQSIGEPGTQLTLRTFHIGGAVGGIGIESKIVAKADGVVHFDELRYIEYE
                     NNSKNKYYIVIGRSAEIHLLDPNTMTILQTANIPYGAKLYKLEGEVVKKGEIICEWDA
                     YNSLIISDVAGKVAYNDIIENITFKYEIDENTGHKDRIIIESKQRTKIPQVLITSPNG
                     DVIKTYDLPVGANVLVDNGQLIKAGDIIAKIPRVVGKASDITGGLPRVQELFEARSPM
                     DPAVVAEIDGIVSFGPKLKRGNKELIITSKTGEERHYLIPITKQILAQEGDFVKAGTP
                     LCDGPISPLDILAIKGPTKVQEYLVNEIQEVYRLQGVKINDKHFEVIVKQMMQKVEIV
                     DPGDTRFLEGEIVDKFTFKDENDKIFNMKVVTDAGDSDLRPGMLITARQLRDINSVLK
                     RRDMNLIQARDAQPATAKQVLQSITRAALQTQSVLSAASFQETVKVLTNASVKATRDT
                     LQGLKENVIVGHLIPAGTGIRQYENLIVGSMDEYEALIESKKEFQNQ"
     gene            complement(51751..55551)
                     /gene="rpoB"
                     /locus_tag="PKI65_00830"
     CDS             complement(51751..55551)
                     /gene="rpoB"
                     /locus_tag="PKI65_00830"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOB77019.1"
                     /translation="MKERIKFGKSLQYETPDLLEIQLQSFKDFFQLETTPENRKNEGL
                     FKVFKENFPITDSRGLYVLDFVDYYVDPPRYTIEECMERGLTYSVPFKARLYLYCTDP
                     EHEDFEAVTQDVYLGPIPYMTPKGTFIINGSERVVVSQMHRSPGVFFQTTRHPNGRLI
                     YSARIIPLKGTWIEFTTDIQSVMYAYIDRKKKVPMTTLLRAIGYETDKDILEIFNLTE
                     EVEVNEENLKNCIGRKLAARVVRTWIEDFVDEDTGELTSLERTEVLIERETVIEEIHI
                     PIILKSDTETILLHRDDIVTEDYSVLFNTLQKDPANNQREALEYIYRQLRNTEPPDKE
                     TALQIIDKLFFSDKKYDLGEVGRYKINKKLGRDTVDINQRVLTHEDIIDIIKYLINLI
                     NNKTDIDDIDHLSNRRVRTVGEQLYMQFGVGLARMARTIRERMNVRDNEVFKPIDLIN
                     AKALSSVINTFFGTNQLSQLLDQTNPLSELTHKRRVSALGPGGLSRERAGFEVRDVHY
                     THYGRLCPIETPEGPNIGLINSLTVYSRINDLGFIETPYRRVVDGVVHINESPIYLTA
                     EDEENYYIAPANTLLDENGLIVEDLLMCRYGADFPVVPREQINLMDVATNQIASVSAS
                     LIPFLENDDANRALMGSNMMRQAVPLLNPEVPIIATGLEAKVAESSRVLINAEHDGIV
                     EYVDAKKIVIRYDLGENDKLASFDDDATVYELTKFLRTNQNTIINLIPIVKKGDKVKK
                     GQVLCEGFATKEGELALGRNLLAAFMPWKGYNYEDAIVISERVIKEDLYTSIHIEEFS
                     TEVRETKRGVEELTNDIPNVSIEATKDLDSNGIIRVGAEVREGDILIGKITPKGETDP
                     TPEERLLRAIFGDKAGDVKDASLKTPPGTSGTVIDTKLFSRLTKNKKEKEKEKHLIEK
                     AEAEFEKVASNLRDKLIKKLLFITEGLVSNGVYNKYKVELIPKGEKFTARNLQRIENY
                     LDINPENWTLDEEKNKLIKQVLHNYEIKYKDFYGKLQRNKYTLSVGDDLPTGIVKLAK
                     VYIATKRKLKVGDKMAGRHGNKGIVAKIVSEADMPFLPDGTPIDIVLNPLGVPSRMNL
                     GQIYETVLGWAGKKLGLNFATPIFDGATIDEINEYIKKADLPDNGQVYLYDGGTGERF
                     DQPTTVGVIYMMKLHHLVDDKMHARSIGPYSLITQQPLGGKSHFGGQRFGEMEVWSLE
                     AFGAAHILREVLTVKSDDVTGRAKAYEAIVKGRNLPEPGIPESFNVLVHELRGLGLKV
                     TFEE"
     gene            complement(55722..56105)
                     /gene="rplL"
                     /locus_tag="PKI65_00835"
     CDS             complement(55722..56105)
                     /gene="rplL"
                     /locus_tag="PKI65_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOB77020.1"
                     /translation="MADIEVLAEELVNLTVKEVNELLTILKEKYNIEPASAPVMVAGA
                     NMGDTGQATAQEEQTEFDVILKTPGPQKLAVVKLVKEMTGLGLKEAKELVDNAPNPIK
                     EKISKDEAEALKKSLEEAGAEVEVK"
     gene            complement(56136..56657)
                     /gene="rplJ"
                     /locus_tag="PKI65_00840"
     CDS             complement(56136..56657)
                     /gene="rplJ"
                     /locus_tag="PKI65_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOB77021.1"
                     /translation="MKKEDKKILLDELTEIINKNDFLYIVDISGLDSVDTFKLRSLCF
                     RENIKLKVVKNTLLRIAMQNSDKDFSEFYHILNGPTAIMYGAIVNNAPAKLIKKFRQN
                     SDKPILKGAFIEGVAFIGDHQLNTLATLKSREELIGDVALALNSSFNKLLSTLQGQGQ
                     KIAGVLKTLAEKE"
     gene            complement(56670..57362)
                     /gene="rplA"
                     /locus_tag="PKI65_00845"
     CDS             complement(56670..57362)
                     /gene="rplA"
                     /locus_tag="PKI65_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOB77022.1"
                     /translation="MTKISKKRKTALTKYDKNALYSLADAMKIIKDITYTNFDASVDL
                     DVVLGVDPRKSNQMVRGSVMLPHGTGKEVRVLVLCGPDKENEAKDAGADYVGLDEYIE
                     KIKSGWTDIDVIICTPDLMPKLAPLGKILGPRGLMPNPKTGTVTMDVGKAVKAAKSGK
                     LDFKVDKYGIIHAPIGKVSFETDKLVDNAKELLTTIIRLKPPTTKGTYIKKITASSTM
                     SPSVKIDPKSIN"
     gene            complement(57396..57833)
                     /gene="rplK"
                     /locus_tag="PKI65_00850"
     CDS             complement(57396..57833)
                     /gene="rplK"
                     /locus_tag="PKI65_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOB77023.1"
                     /translation="MAKEVVGIIKLQIKGGAANPAPPIGPALGAKGLNIMEFCKQFNA
                     RTQNQAGKLLPVIITVYSNKSFDFVVKTPPVVVQLMEAAKIQKGSSEPNRIKVGKVTW
                     EQVRQIAQDKMNDLNCFTIESAMKIVAGTANSMGISIIGNPPF"
     gene            complement(57850..58404)
                     /gene="nusG"
                     /locus_tag="PKI65_00855"
     CDS             complement(57850..58404)
                     /gene="nusG"
                     /locus_tag="PKI65_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOB77024.1"
                     /translation="MQDIDNDLRKWYVIRTITGKEKKVKELLEIEIDKLNLHNYISRV
                     LIPIEKVYQIRKGKRITKEKNYFPGYVLVEAILDGEVINTIKSIPGVLNFLGDKDKPT
                     PLRPAEVRRILSKVDELNDKGEEIEVSFLVGENVRIIDGPFSSFIGTVEEINEEKKKL
                     KIGVKIFGRKTLLELSFMQVEKES"
     gene            complement(58431..58613)
                     /gene="secE"
                     /locus_tag="PKI65_00860"
     CDS             complement(58431..58613)
                     /gene="secE"
                     /locus_tag="PKI65_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOB77025.1"
                     /translation="MKKLIRQLKEVRDEFLYKVTWPSWDELIEGSILVAIASLIIAVV
                     VLVMDLASQGIMGLIY"
     gene            complement(58629..58701)
                     /locus_tag="PKI65_00865"
     tRNA            complement(58629..58701)
                     /locus_tag="PKI65_00865"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58666..58668),aa:Trp,seq:cca)
     gene            complement(58726..59913)
                     /gene="tuf"
                     /locus_tag="PKI65_00870"
     CDS             complement(58726..59913)
                     /gene="tuf"
                     /locus_tag="PKI65_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOB77026.1"
                     /translation="MAKEKFERTKPHVNIGTIGHIDHGKTTLTTAITQILSEKGMAQA
                     KSFYDIDNAPEEKERGITINTSHIEYETDNRHYAHIDCPGHADYIKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLAHQVGVPAIVVFMNKVDLVDDPELLDLVEMDVRDLLNF
                     YGYDGDNVPVIRGSALGALNREPQWVEKVLELMDAVDNYIPQPLRDIDKDFLMPIEDI
                     FSITGRGTVATGRIEQGVINTGDPVEIVGLSEEKLKSVVTGVEMFRKILDRGEAGDNV
                     GLLLRGIDKDEIRRGMVVAKPGSIHPHKKFKAEVYILKKEEGGRHTPFKNHYRPQFYF
                     RTTDVTGEITLPDGVEMVLPGDNISIQVDLIYPIAMNKGLRFAIREGGRTVGAGQVTE
                     ILD"
     gene            complement(59943..60014)
                     /locus_tag="PKI65_00875"
     tRNA            complement(59943..60014)
                     /locus_tag="PKI65_00875"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59980..59982),aa:Thr,seq:ggt)
     gene            complement(60028..60100)
                     /locus_tag="PKI65_00880"
     tRNA            complement(60028..60100)
                     /locus_tag="PKI65_00880"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60065..60067),aa:Gly,seq:tcc)
     gene            complement(60107..60188)
                     /locus_tag="PKI65_00885"
     tRNA            complement(60107..60188)
                     /locus_tag="PKI65_00885"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60152..60154),aa:Tyr,seq:gta)
     gene            complement(60197..60269)
                     /locus_tag="PKI65_00890"
     tRNA            complement(60197..60269)
                     /locus_tag="PKI65_00890"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60234..60236),aa:Thr,seq:tgt)
     gene            complement(60383..60676)
                     /gene="raiA"
                     /locus_tag="PKI65_00895"
     CDS             complement(60383..60676)
                     /gene="raiA"
                     /locus_tag="PKI65_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOB77027.1"
                     /translation="MKVNINPIHFKTDKKLDEYITDKINKLSKFHQEIISSEVILKLD
                     NTSAPENKIVEIRLIIAGNDLFAIKKAKTFEEATDLAVDALRRQIEKTKDNWK"
     gene            complement(60706..61605)
                     /locus_tag="PKI65_00900"
     CDS             complement(60706..61605)
                     /locus_tag="PKI65_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOB77028.1"
                     /translation="MDWIKEYLNYLENNQRYSFNTISSYRIDLYQFDEFIKNTFDIDI
                     LEVNRIHVNDWIMNLSQSGLKASTINRKIVSLSSFYNYLLNKEIISKNPIKNISKPKI
                     PMRIPQYIKLKDLSPYDFENTNKSEIKNDYEAIRNILIIEMLYQTGIRRSELINLKNE
                     DIDLNRKTIKILGKRNKERYVPFTNNLKKIIIEYLDIKNIFFNNKEVDDKFFLLNSGK
                     KLYDKFVYRTVNNYLSSINYISQKSPHILRHTFATHLLHEGADLNAIKELLGHSSLAS
                     TQIYAHSSIERIKQVYSKSHPRS"
     gene            complement(61658..61855)
                     /gene="rpsU"
                     /locus_tag="PKI65_00905"
     CDS             complement(61658..61855)
                     /gene="rpsU"
                     /locus_tag="PKI65_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOB77029.1"
                     /translation="MLIVELKEGDSIDKALKKLKRKYEKTGVAREVKARQFYVKPSER
                     EREKKSRALYMQRRITKSMNA"
BASE COUNT        21327 a         9750 c         8925 g        22067 t
ORIGIN      
        1 ctgcaaaact acattatgtg gtatgtagtg atgggtaaat acctcacagg caagactgta
       61 agcaatactg ggcgaatgct taaccttgca tcctcggaca ggcgggcgtg gtatcggtat
      121 agagaattag tgaatataac atatgttaat tgaaacatat attaattctt caatccaccc
      181 acgctgtcat tacatttata aataacctat cattaaaaac ttacataaaa gataaatctt
      241 actatgtgta tctaacatta aaattatcca taaattttta tatgctagaa ctcattatga
      301 ataagtaaaa aatttaaatc taatcagaag taatatttat tagtattgcc aataaaattt
      361 tagtatataa ataagtttta aacttataat cttatgtttc tttaaagcat agcaagataa
      421 taaataaaaa atacaaagta tttgtatttt aaaaaaaaca tcttatatga tagcataact
      481 tatttctttt gtttctttag atttactgtc attaggttgt tctatggctt taactactag
      541 ctcagatata tccatcgttt cgatacgcct atccctgaca aatgtttttt tacaaagtgg
      601 gcaagcagtt atcaatacat ctggattaaa tttagcaaaa taagacattg tattataagc
      661 taccgtattt ttagcttctg caggtaaatt caccccacct aatcctgcac cacagcataa
      721 tgctctatct ttatctttat ttttttgtag tggtattcta tgagcataat gttctatcac
      781 ttcacgaggt tcttggtata tgttagatcc tctaccaagt tcacatggat catgataaat
      841 agctgttaga tttagtttgc cttctggtaa tttattttgc tttattaatt cttttatata
      901 attggcatga aatttaatat ttatatctgg taatagataa tcttctttaa aaactttata
      961 acagtatgga catgataaaa taatattttt ggcaccagaa gcaattatta gttctgtatt
     1021 tttgcttatt aactgtgatg cggaatcata ttgtcctgct agcataagag gtctcccaca
     1081 acatatagat ttgtcatcat ccataaacca aacattttct cctacgtaat caaagatttt
     1141 agtcatagat tttttgatat taggagttaa atgtgtcatg caacctgcga aatatatcgt
     1201 atcagcagtt tgttgctcag gttttttggt ataagcatat gaaaaggtta atggtttact
     1261 ggcttttata cgtaatgcag ttcttaaatc atttaaattg agatgtactg gacactctac
     1321 ttggcatctt ccacaaatca aacatccaaa aaggggtttc tctgcatcta ccttatttct
     1381 aataaattgc aaaaaataga cagattgtac atcagaaata ccagcatcaa atagttggca
     1441 agcatctaag cagataccac atctcgagca agaatataat tgtatctgtg catagctctc
     1501 ccatgttttt tttgttttta tgccagcatt acgtaaaaaa atataataga tctctgtagg
     1561 tatatgcata tatctcgaaa atggtaaagc tataaaaaag aaacaaagag caaacgaata
     1621 taaccaccag cttggttcat atatggctga tacatttatc atattcccta gccaagctcc
     1681 tgtggtatta gttaaaaaac taccattatt atatacagct gcactacaac tttctgctaa
     1741 taaacgaagt ggaaagatag accaaagtga ataaagtgcc attttgtcaa atgtctttaa
     1801 tttggtagta cgtttcatcc caaataattt tttataaaat ctctttgtta tcgcaagaac
     1861 tacaccagaa agaataaata aaagtaaaaa atccattact gctgcaaata ttttagcaat
     1921 agtagtagca tgcactgttt cgaaatatct aaaaaatata gcataataaa atggaaaatt
     1981 taacccacct ttagctacca ctacttctaa atgacctatt actatcaata aaaaccatcc
     2041 aaaagctagg ctcatatgca tatatcctaa aactggattt tttttaaaaa tacttaaatg
     2101 caataaccca tcattgatgg aatccttaat tgctttccat actttatttg tagtccaaaa
     2161 ctttttaaat cttactttat catatttaga taatttaaca taccatttta tatatatata
     2221 aataagagct gcgaataaaa atatagtacc tatcaaaaat ggatattgaa aatatgtttt
     2281 cataataata tcattttata ttttaaaata tttctcttaa tattcattac tacattacgt
     2341 aattgaatat ctctgggaca taccaaagta catttaccac aaagcataca cttttctaat
     2401 tctttttcta agctttctat ctctcccctt ctaagtagta agctaatgcg cctaacatta
     2461 aaagtagtat gattcccagc actacagcta gcagtacagc aaccacagct tatacaccac
     2521 tgaaatgagg gttcttcttc taatacttta tttagtaaat caagttctaa tttatcataa
     2581 tctatgtgta aatgctttac tttgagaaaa tctaaatcac ccataattat ttatatatta
     2641 ttttattatt tttttaaaaa ttcttaagta aattaaaaaa actaatgaaa aaaccaaaaa
     2701 aatagtactt agcattgaat attattagat tgcttagcat gcaaaatatg tgatttcccc
     2761 cacagtggtt tagaaattta gtttttgtta ttatgagatt attaaacata atatattttt
     2821 tcaattaatt agatttgata tatgataata cagataaagc tgttcctcga gcatggtcaa
     2881 tagtttcacc tactgacatt ggtcctatag aagtaccagc taaaaaaacg ccttcttttg
     2941 cagtcaaatt tcttaaatta tgaggattta taggatcgaa aaaaccgtgt tcatttacat
     3001 tcaagtcatt gtgatatttg aattgcttag aaaaatcaga tgcttccatg ccaaccatta
     3061 aaactatcat atccgcactc atctgtaatg gcctacctat taaagtgtct tcggctttga
     3121 tttgaatttt tttatctatc atttcacttg cttcagataa tctaccacga acaaattgga
     3181 tgttataatc tctctgggca ctaagatata gatcttcgaa taaaagtcca tacatacgca
     3241 aatctatata aaagcaaaaa atttcaacta agggcaaaag ctttttagtt tcaatagctt
     3301 gtttgatggc agtaatacag cataaacgcg agcaataagt attgctaacc ttggcatcgc
     3361 gagatccaac acaatgaata aacccaattc gttcagggac cttaccacta ggagtaataa
     3421 tctcttgttt tttaaacatt tgttctagct ctacagaggt aatcacatta tcatatatac
     3481 cataaccaaa ctcttctttt aaccgtgcat cgaatgtgtt gtatcctgta gctatcacga
     3541 tagcatctgc tggtatagta ataccatctt ttgtaattac attatagatt ttttttgaaa
     3601 aatctatctt ggacacatgt gcatttaata gaagttgtaa gttgggatct gttataatat
     3661 attggtaaga atttaaaaga tcttcggcat tataaaagtc aggaaataag taagcataat
     3721 tttttatatt gccaccgatt ttagaacttt gctctatcaa agtaactggt aagttagctt
     3781 gtagcaattg cttagatact tctaatcctg ctattccagc tccgatgact actatctttt
     3841 tcatatttat attttttact ttggatttta tttttttata cattgagagc gcaaagataa
     3901 atatctatct ttgtcaaact ccacaccgat tttttctaac aatggtaaga catctgtctg
     3961 atgcataaaa agaccaattt ctattggatc ataaccaagc aatattgctg ctacctcctc
     4021 gtatgttaaa acaggtattc cattcccatc aacagaataa acttttcctg tagtttcttg
     4081 tatcacgtat tgccaacgat ccataaacat attacagccg ggacaattag taataataaa
     4141 gtcaggttga taaggttcca tgctttcaaa ttttacctgg gaatgagtga gtgaataacc
     4201 cctagtttct tttataaaat attgtctaaa gccaaatcca cagcaatgtc ttcgagcagg
     4261 ataatctata acttcaccac cccaagattt tatcatttct gacaatactt tgggaaattc
     4321 tgcaccgcct actccatgct ggggaaaaat tttagcatag tggcatccta catgatctac
     4381 cccttttagt ggttgtcctg tttttttgtt tatcaattta tagatgcact gcttggctat
     4441 ttcatctcta tataaataga taatatcact ggcatgagca atagtttttg gtagttcaaa
     4501 agttcttttt gtagtcttat aaagattttc tatagttttt tctaatgttt ctggaaattc
     4561 atgccacata ttcaaagctt cagtcattac accaaaagta gttacacagg atattacaaa
     4621 attagaatat ccagactcat tcattaatga aaaatgcctg gctaccatgg tatgtacagt
     4681 ttctaaaggt atgatgtcac tatgatatcc tattcctgta caggttgtta agtctggatc
     4741 atctttataa tttagattta gatttttgct tagaatgttt aaaaacattt gttctgcgcc
     4801 cggaaaaaat gtttgtctga tacaacttct aaaaaagaaa taattgtcac gaggaatttc
     4861 ttttatatat tcactccagt atattctatt tattgaaact tttatattag catccatgat
     4921 tattcatttt tatgatagtt tttatattca ttgaccgatg tctctatttg gtcccaaaaa
     4981 tctacagcac cagtcccgtt tagaatagaa tgtatctcat tcatcacttt atcagaaatc
     5041 tttctaacag gtccaggacc ttcacgatcg atatttacat taactaagga atatgctttt
     5101 tcgcgattgt tgaaaatcca ttcccataca ggtccttgct cgggatgtgc ttcacgcgtt
     5161 acttttgatg gatgtacaca atagcccaaa tcataaatat tttgtcctat tgtttgcttt
     5221 attattattt gatttcgccc cattttactt tttacaaaaa gtccaagctt ttgagaccaa
     5281 tatctcaatg cactgatagc tactcctgga gcattttctc ttggacaacg agctttgcac
     5341 gacatacatt gtccacaata ccaaatataa tcggacgata gcaattgttc taactcattc
     5401 tcatcttttg ataataatat tttgatgaca tatcttggct cataatcata ataatctgat
     5461 gctgggcata tggctgtgca tacaccacaa ttaatacaag cattcaaatt ttcttttatt
     5521 cgaggatctg aataaatttc ttctataggt atcattattt tatatattac taaaaaacta
     5581 aaaaaaaatt ttccttaatt aaatcttttt tacaaaagaa accaaaatat tttaataaaa
     5641 aaataaaaat gaaaaatatc attttttaat ataaataaat aatccattat ttgaataaaa
     5701 tcaaaattta ggtattttta ctaacaattt ttaaattatt aatgcttgtt taataatatt
     5761 attcaatatt acttttatta agatattttt ctaataagat ttagagaatc aaaaaaagga
     5821 aaagactaaa tatttgttat attttaagta ttttatacta tcttttaaag aaaaaaaata
     5881 ttaaatttaa gctatagctt taattactcc tgcctaattg taatttcaaa ctttttcaat
     5941 aattatttaa aaaattacaa taataatatt cttatttata ttatacagta caaatataat
     6001 ttttttttat aatgttaatt ttaaattaag ttttaattat taaattcatt ttccatatca
     6061 gtagttacat ctagtgggga atcatcttta gaatatgaga aaactttttt ataaaatatt
     6121 aacattatca agacgcctgt agtgatagat gcatcagcta tattaaaaat aggttgaaaa
     6181 aaaacaaaat ttttacctcc ccagatagga aaccaatcgg gcaaagtggt attgataata
     6241 gggaaataca acatatctac tactcgtcct tgcataaaag gggcataacc ctgacctata
     6301 gggacaaatc ttgccggttg tgtatcaaag ccatatatac cactttcact aaatatcaat
     6361 ccataaaaca tactatctat aatattacct atagcacctg caaaaatcaa agctatggaa
     6421 taataaaaaa gtgagtgatg atttttttta aaacttttaa ttaaaaacca taaaataaca
     6481 gcagccgcta ctattcgaaa aatagttaat atgtatttgc caatagagcc accccattga
     6541 atgccatatg ccataccagc attttcaaca aaatacaggc aaaaccaatt acctatcaag
     6601 catacttttt cgcctaataa aaaatttgtt tttatatata tttttaaaac ttgatcacaa
     6661 aataatataa ttaaaattaa aattaagaca tttcgcttaa ctgaagtttt gtgatcaccc
     6721 atagctaata atattttaag attctattag tgcttttagt tttttaatga aaattattta
     6781 taataaaatt ttgaatattt attaaataac ataattttaa aagattatcc ttcgatttct
     6841 tcttcatttt ctttattaat aggtatctcg cctctctgca tttgcagttt agcttctata
     6901 ctgagagtag cgtgtggtac agcttttagt ctttctttat ctataagttt tcctgtaact
     6961 ctacatatac cgtaagtttt attttctata cgtacaagtg ctaattcgag atctctcaca
     7021 aatctttcta atctagctat caatttagca ttttcctctc tcattagatt ttctttacta
     7081 tcctcttgca atctaaaagt tagatttgtt tctatttcac catcactact catactttca
     7141 cgcaatattt gaaggtctga tttagctttt tctaattttt ttaaaattat ttgtttaaac
     7201 tcctctagct cttcatcact atagcgcgtc ttttcatcaa aattattatt tgttgccata
     7261 atcaattttt tttaaaacaa ttatattttt tcaatatgta ctaaaacatc aatattatca
     7321 tctactgtaa taacatctgc ctctaatcca tttatagaaa tatttttaat ttctttagct
     7381 aatatttcgt tgcaaatgta agaataattt tcaaatatac ttttgtctac aaaaggatca
     7441 gacgaataga aaaacactct tattttgtca gtaacgtcgt aatttttgtc ctttcttaaa
     7501 ttttgtattc tgtttacaaa atctcgtgct actccttcca ttcttagttt ttcagtaata
     7561 gtaatatcta aagctacagt taaattatta tcatgtgcta ccatccaacc aggtatatcc
     7621 tctgtgaaga tctctacttc ttctaataat atttcgatat gtccttctga agtagttatc
     7681 gtgatttttt tattcgtttc tatttcattt atcatattat tatcccagtc ttgaataata
     7741 gctgatattt ctttcatcaa tctaccatat ttaggcccta ataatttata atttggttta
     7801 gcttttttaa ctaaaaaatc attttgcaaa cttatatatt ctaaatcttt tatattggtt
     7861 tcatgcaaaa tcaaatgctt taccttttct aattgcgtaa tagtattttc atcagcaggt
     7921 attatagcct tcgatagagg ttgtctaact ctaatgtttg ccttttttct gagtgaaagt
     7981 atcatagaac ttatcttttg agctagttcc attctatctt ctaattcttt atctatataa
     8041 tttgaattat atataggaaa atctaataaa tgaacacttt catgtgattt tttaatagta
     8101 acttcattca aatctctaaa caatctctca gaataaaaag gtgcaatagg agataacaaa
     8161 attgctatgg tttctaaaca agtatataat gtttgataag ctgatatctt gtctatggaa
     8221 taatcaccct tccaaaatct tcgacgcgac aggcgtacat accaattact aagatgatcg
     8281 atagtaaatt gttctattaa tctgccagct ctcgtaggtt catagtcttc ataagcctca
     8341 tctacatttt ttattaaact atttaattct gataaaatcc atttatctaa ttccaatctt
     8401 tcttcaaaag gtatttctgg ctcacaataa gtaaatccat caatatttgc ataaagagca
     8461 aaaaatgaat aagtattgta aagagttcca aaaaatcttc ttatcacttc ttttacacat
     8521 tcgatgtcaa atcttagact atcccatggt tgtgaatttg taataatata aaatcttaaa
     8581 gcatcagcac tataatgatt aattatttcg aatgggtcta cgacgttacc aagtctctta
     8641 gacattttaa atccattttt atctaaaact aatccattag ctataacatt tttaaaagcc
     8701 acactatcaa aaatcattgt agctatagca tgtaacgtat aaaaccatcc acgagtttga
     8761 tctacgccct cagcaataaa atcagctggg aaattttttt cgaaaacatc aatattttca
     8821 aaaggaaaat gtaattgagc ataaggcata gctccgctat caaaccatac atctatcaca
     8881 tctggttcac gatacatagg tttgcctgag ggtgatacaa gtattatatc atctacatat
     8941 ggacgatgca aatctatttt tttataattt tcttctgagt catcaccaat tacaaaatcc
     9001 ttgaaaggat ttgatttcat ataaccgcac tcaatgcttt tttctatttc tttaattagc
     9061 tcttctacag atccgataca tttttcttca gttttgtcag ttgttctcca tataggtagc
     9121 ggtgtccccc aaaagcgcgt acgactcaaa ttccagtcta ccatattttc taaccaattg
     9181 ccaaaacgcc ccgttccagt gaattcaggt ttccaattaa tagtcctatt gagctctatc
     9241 atcctctctt tgtatgctgt agtttttata aaccaagaat ctaatggata ataaataata
     9301 ggtttgtctg tacgccagca atgaggataa ctatgttcat acttttcagt agcgaaggct
     9361 ttattttcgc gttttagttt tgctattatt tctaaatcta caattttttc tttttctatt
     9421 gaatgatcag tataatattc ggcttttaca tatttaccag caaattctcc catttctttt
     9481 gtaaatttac cttgcttgtc tacaagagtc aatgcaccta tatcgtataa tttatccatt
     9541 ctaaagtcat ctgcaccaaa actgggagct atatgcacta tacccgtacc ctcctcagtt
     9601 gatacgaagt cacctataat cactctaaat ggatctcccc tatcaggttt agcatagggt
     9661 aaaagttgtt cgtatctaac atttaataaa tctaaacctc tgaaagtacg taaaatttta
     9721 tatggaataa tattttttgt tggatcaaaa gattcgaaag gtaaattttc gttattagga
     9781 ctaaaccatc tatctataac atttcgagct aaaataactg tttgtttttc ttttgtatat
     9841 ctattgatag ttatcacttc tgcatattct atatcttccc ctacagctaa tgctgtattt
     9901 gaaggcaagg tccatggtgt ggttgtccaa gctaaaaaat atacatctcc ttcagcacca
     9961 ttaaataaaa attcagattt ttcatttttt ataactttga attgggctac tactgagtag
    10021 tcttttataa ttttataaca ccctggttgg tttagttcat gagaactcaa acctgttcct
    10081 gctgctggag aatatggttg aatagtataa cctttataca aaagaccatt attataaagt
    10141 tgcttgagca aataccataa tgtttctatg tatttattat caaaagtaat gtaaggatga
    10201 tcgagatcta tccagtatcc catgatttta gttaattcct cccacttatc tttgtatttc
    10261 attacttctt ttctgcattg attgttaaaa tctgcaatag atattttttt gcctatatct
    10321 tcttttgaga ttcctaattt agactctaca gctatctcta cgggaagtcc atgagtatcc
    10381 catcccgctt ttctatttac ttggtatcct tttagagttt tgtatctaca aaaaagatct
    10441 tttattgtac gggacattac atgatgtata ccaggcatac cattagcact aggtggtcct
    10501 tcgtaaaaca caaaacaagg ttgtccctcc ctatttttaa tacttttttt aaataaatca
    10561 tttgtttccc aaaaatctag cacttcatta gctatattag gtaaatttaa gctcttatat
    10621 tctctatatt ttctcataaa atataattaa ttttgcaaaa ttacaagaaa aaaatgaatc
    10681 taaatactaa aaaattaaaa atgattatta aacatgtcaa aaaataaata ttacgtggta
    10741 tggatcggta gaaaaccagg aatatatacc tcctgggcag aatgcaaggc tcaagtcgaa
    10801 agttatccag gagctaagta tatgggattt actacttatg atcaggctct attagcatat
    10861 caagcaccag tgccttcgcc tgatgtagta aatcctattt tagaacaaat acaatatttg
    10921 tttcctcctg acagtatagc tgtagacgct tctgtaaata tgaaaactgg cactatggag
    10981 tatagaggca tacatttatc gtctatgcaa ttaatttttc acgtaggtcc ctgggattat
    11041 ggattaaaaa gcaataatat agccgaattt cttgctttag tacatggatt gcaatggcta
    11101 atgcaatacg acttagatgc accagtatat tccgattcta taactgctat ggcgtgggtg
    11161 agaaataaaa aaataaattc taaaactctc aaaaaaatgc caataccaca aataatagaa
    11221 aaagtcgata acgctattgc atggctacat gacaatccta atcatgctga ggtgagaaaa
    11281 tggaatacag aacaatgggg agaaataccg gcagatttta atagaaaatc ttaaattaat
    11341 tttaataata aaaaattatt ttataatttt gcaaaaaggg cggttcgtct aagggctagg
    11401 atctcagatt ctcattctga agataggggt tcgaatcccc taccgcctac ttgcatacac
    11461 tcaactaaat gtaatgcatt aggaataaat ttttaatgca ttagtattca agttttaatt
    11521 taataattct gataaataga aaagattttt tctatttttg caaaaaataa ttcaaaatta
    11581 tgaaaagaaa aaatgaattt tatagttatg ctactaagca tatcggtatt agtagtcaaa
    11641 ccttagacga ctacataata gcatctaatg gatacatatc acccaccatt atagaagagc
    11701 gacaacttaa cgttgcacaa atggatgttt ttagtagatt gatgatggat agaatcattt
    11761 ttttaggtgt acctatagat gattatgtat ctaatatagt aactgcacag ttgcttttct
    11821 tagaatctac agatccacaa aaagatgtat ttttatacat gaatacacca ggtggtggtg
    11881 tatatgcagg cctagggata tatgatacta tgcaatatgt tgccccagat attgctacta
    11941 tttgcacagg catagccgca tcaatgggag cagttttgct ctgtgctggt actaaaggca
    12001 aacgttcagc tttacctcat agtagaatca tgatacatca acctatgggt ggtgcagagg
    12061 gacaagctac tgatattgag ataatagcaa aagaaatcaa aaaattaaaa gatgaattgt
    12121 accaaatcat ctcataccat actggacaac catttgaaaa agtagaaaaa gatggtgatc
    12181 gtgatttctg gatgcgatca gatgaagcca aagaatatgg catgatagat gaagtactga
    12241 ctaaaaataa agaataaaaa tcaactattt taactattat aatatacaaa aactctaaat
    12301 ctcaaataaa tctatataca aagcataatt aactttcata ttatagatta gttggtaaga
    12361 agaccttgag atgtgatgta ggaaatagtg atattaaaaa aaattattag ttgtaggttg
    12421 atatttttga tttatgtata attaattact tgcaatgtaa aattaatatt ttcctattca
    12481 attttcattt tgtgtaatga tttcattgaa ctcgggtaaa tctaatagtg atttcagata
    12541 attatacaat aatctcacac ctacacctgt gccaccttgg ggccaatacc cccaatgctt
    12601 agttatataa gctggtccag caatatctaa atgtataaaa ggaaaatcag aaaaaaattc
    12661 taaaaatttt ccagccgtaa tggctcctgc atattcgtta cctgcatttt ttatatcagc
    12721 tatatcactt ttgataggat tgaaatattc ttcccaaagt ggaaattcta caatgcgttc
    12781 gtaagtgtcc ataccaattt tttcgagtct ttcgaaaaat gctgaagcct tggattgcat
    12841 gccagctatt acataatgtc cgagagcttt atgtgcggct ccagtaagtg ttgcaatact
    12901 aataacaagt gtaggggcgt atttctgagc aagacatagt gcatctgcta aaatcagtct
    12961 accttctgca tcagtattca ctacttccac cgttctacca tccatcattg taataatatc
    13021 accaggcaaa agtgaatttg gtcctggcat attgtctgtt atcggtatca aaccaatagc
    13081 atttactggt atatgattta tagctaaagc atacattaaa cttgctacta ctgcaccgcc
    13141 agccatatca gatttcattt catgcatatt tttggctggt tttagtgaga taccaccagt
    13201 atcaaaagta atacctttac ctacaaaaat aaagggttta gtatttaatg cttgatcatc
    13261 gttatggtgt aaaattacaa aatatggtgg tgtagagcta cctttattga ctcctaataa
    13321 accacccatt ttcatactct ctatttgctt tttttctaat atttctatat tgatagctat
    13381 accttcaaac aagtttaata tttcctgtac aaaaatagat gaatataaat ctgatggagc
    13441 tctattcact aaatctcttg cccaaaagac agatttcact aaatgataaa gttcaatgaa
    13501 ataggcttca tatttatcag aaacctgcaa taaatattta acttcatttt ctgtttttaa
    13561 tgatttatac tggttaaacc tatagctatt taaaaacaaa ccttctacaa attcataaaa
    13621 ataaattaat acctcatcat tacattgtaa aattaattct gataaattct catcttgaac
    13681 tattttagaa actttactac caacttttcg tatttcttcc ttatcacttg gttctaaaat
    13741 tactattatt tctaaatgag tagatctatt gaatactact acattattgc tacgatttaa
    13801 ttctttattg ataatatttt tatctttatc atctatctca ctgggtagtt gatctatttt
    13861 agtaccaacc catactagat ttttagtagt actatgtata ttagaaaata ttaattcaaa
    13921 cataaaagat tattttaaat taattggttt ttttgccaaa attaatattt agattttttt
    13981 aattataatt aaaaaaaact ctattaaaac tattatcgtt aatttttttt aaaaattatt
    14041 caacgatttc taataattct actttaaaaa taatggtaga tccagcaggt atttctccta
    14101 tgggtctatc gccatatccg agttcagaag gtatatataa ttcgaatata gatcctaccg
    14161 gcatcaaggt taacccttcg gcccagccag gtatcacttg atttagttga aattctatag
    14221 gttcacctct ttggtagcta ctgtcaaata ccttgccatt tagaagtttg ccttcgtagt
    14281 gtacacgcac tttatcagca attgtaggtt tgggaccttc ccccattttt atcactttat
    14341 attgcagccc agatgttgtt tcataaacat ccggattatt tttattattt tctagaaatt
    14401 ttttaccaac ttctttattt aagttagctt cttctagcct tttagcttct gctttttgca
    14461 tagcctccct ctgtaatctt tgcatagcag cttgaaactc ttcattagta aataaagtat
    14521 ctgcattatt taatacatct tcgtacccgg cttttaataa attgatatct atatctaagc
    14581 cattatttat aaaattacga gccagatcat tacctatcaa gcaactgata gtatctctga
    14641 aattattaaa gctaatggta gatttggttt cttgattaat ttcattgtca ttagtagtct
    14701 ttgatatttc tttagtagaa ctgcatgaat aaattaaaaa gataaaaata aataaaagac
    14761 ttggagtttt tgttttcata attttttata caaaaataca tcaaaaaata aaactgcaaa
    14821 aaaaataatt atttaatcat ttactcttaa aaaaaaaata acaaataaac taaacaatta
    14881 atgactaagg actaacaact gggatcataa attctatata aattttgaat tctgatttac
    14941 acctttctca tcctccctgt gtcaacttgt cattcatctt aattattact ttgaggttta
    15001 gttttatttg attaattaat atacttggat ataataatat ttgctcatta taaatcaaag
    15061 aattaattac aatactatct aataatagtt atagtaccat gaatctcttc gattttgcct
    15121 cgtccataat ttaatctaaa gatataataa tatgtacctt catctagcct atcaccatcc
    15181 caatcgccct gataattttg actttcataa actcttttac cccaacgatt atagatttta
    15241 atttcactat taggataaaa ttcgatattt gtaatgtaaa aagcatcgtt tataccatct
    15301 ccattaggag taataacatt tggaatatta atttcacaat cttctactat aatagtagta
    15361 ctatcagaaa tggtatcaca accaataact tttacgttta tatcatatat acctggtaga
    15421 ggtttgtcta aaatatagtt tgtgtcattg cttccatcag accaaataaa ttcataaaga
    15481 tcattattaa attgagaaac cgaaagggta ataaattcat gactacataa caatatattc
    15541 tgtggtaaat caactatagg ggtaggtatt atttttacat taatagtatc tgcatcgtca
    15601 cacatatcat aagtactatt agtaacactg acccaatata ttccgctttc taaaacatct
    15661 atcatactgg tagtatggcc tgtagaccat aagtaagaaa acactgagtt ttgctgtgta
    15721 gcatctagtg caaaaggtcc actagtgata caagtatcgt taccaaggtt tacttcagct
    15781 tttggtaata aacgtaccat tatagtatca taatcgatac acatgtattc atcagttcct
    15841 ataatagaat aataagtaat ttcttgtgtg ggatggatat taatactacc catttgacta
    15901 ccagtactcc agtgaaagaa ggcaggaaat tgagttgtat tagctataag ttgaccactt
    15961 tcgccctcac agataatcgt atcacccgtt atatatactt caaatggcat atgatttatt
    16021 atatatacag tatctctgat agtatcgcta ccgatacatg gatttaaact tggtactttt
    16081 attattatag tttcagtaga ttcatctaaa gtgtctgcta tagcatatat gtttatacat
    16141 tgttcgtacg tgtcaggtgt aaaaataaac tgggtaggaa taggatttcc attttcatct
    16201 atgtaatcaa ctccgtttat tgctgtacca tctaaaaata tcgttaatgt atcatttgta
    16261 ttgatatcaa aacttcttgt gaaacatatt tgagtatatg aaccacaatt tagcccctca
    16321 attatcgaat tgtcaggaat agattcacta atattaatat tttccatttg tatatcataa
    16381 gaattagccg agaagctacc agcttgcaaa aaaactccac tatcataaga atgatcagtt
    16441 gcatcagcta tagcgagttt aatgtggtat gtttcgcaag gtaccacgtg tgcaaatgct
    16501 tttaatggca ctgtaaatcc atcaaattgt atagtctgtc ctccagtatt atcaacataa
    16561 tattgcgaat aagctaaact agtgcagcca gatgcagaat agccactgcc aggatatcct
    16621 ggattcacag tattaatagc gacaggaaga ttagtatttg gtattattgc tatatttttt
    16681 ttgttataca tacctccact aggatcaggc cctgttaata aaaaagcaaa cacatcatta
    16741 aaagatgaac atacaaattc tggatattcc tcgctagcaa aaacataata aaaactaata
    16801 gtatctgcct gtggtataaa gtcaaattct aaaacagatg catcattagt aggtactcca
    16861 ataatagcag ataaatcagc gtcaccaggt gtatcgacac ttacagaagc actaccagaa
    16921 ttattagggc cgatagctac tgtagctcca ccggttgtta acaagacacc atctgtgaat
    16981 ccaatatttg ttgtaccata aaaatatgct ttctgagcat tattacctgt aaattgcaca
    17041 ttagtaacag taataccctg gcctattaat atattttgta ctaaatcaaa tgcagaatat
    17101 gatacgtcta catttaattg gctccataca ggtaaagaaa acattattat acttaaaaat
    17161 aataatattc taatgaaatt gtaaattttc ttcataatta ataaataaca gcatacaaaa
    17221 ttacaaaata atattatata aatgttaaat aaaacatcta attattcatt atataccagg
    17281 caaacaatgt ctgacatcat tatatactgt tttatcccct agttgataag cttttacaaa
    17341 attttcacac catttatctc tatcattgaa tatacgataa attttgccta gagatgtata
    17401 cgcctgtgca taagtgctat ctaattctat agttttttcg taataattga ttgcttcttg
    17461 ataattactt tcattaaata agatattccc cattaaataa taaatttcag gatcattagg
    17521 tgtgagtttt acagctttat taagtttttg cttagcagtc tcgtcatctc ttttgtaaat
    17581 atgagtgata gcatctaatt ttagttcttt taccttatct gctttgcttt ggcaactaat
    17641 aaatgttaat aatataatta atgataataa attccctttc attttcatat tttttaatat
    17701 taatcataac cagatataat tttgtctata atatttaatt tgttctctaa gagtccttta
    17761 ttttctgcct ctactatcaa tcttagatat ggttctgtat ttgaaggtct gatattaaac
    17821 caccaatcta cataatctaa tctatagccg tcaaagtcat aaaaagcaat tggtttttgt
    17881 tcattaaaaa agtaatcttt tacagcattc atagcatcga gtttacgatt gattttataa
    17941 ttaatctctc ctgaattttc atactttttg attcttgcta ttatttcaga tactttataa
    18001 ccttttttta caaaatctga gatgatattg gtgattaata tgctagctaa taatcctgaa
    18061 tcactgtaat aaaaatctct aaaatagtaa tgaccggcta attctccacc gtaaactcca
    18121 tcaatttctc tgagtttatt agcagcataa gctctaccga cacgccacat tactattttg
    18181 ccacccatag gttctacata ttctgctact gacctagaag tacgaatatc ttgcaaaacc
    18241 aatccttttt ctccacgctc ttcgagaaaa taatgaccta ataatgctat cattagatca
    18301 ggggaaataa aattactatt ttcatctaca aacattactc tatcagcatc tccatcaaaa
    18361 atcatcccga aatcatcttt tctttttgca acattttgtt ttagagcttc tagatttttc
    18421 tcatccaatg gattagcttc atgattagga aaattgccat ctaattcatc aaaaatataa
    18481 taagggccct catcaaaaat ttgatgtata aataaaccag ccattccgtt gctacaatct
    18541 atagtaaaat taagatccaa atctggtttt aaatattttt ttaaaaaatt taagtattca
    18601 attttgaaat caaactcctt ataagtacca ttttttacta tttcgatctt tttattctct
    18661 tttattaatt tttctatttt atttagtcca tcatcatacc cgataggcac agcatttttg
    18721 cctgaaaatt tcaatccatt atattctttg ggattatggc tagccgttat catgatagac
    18781 ccatcaaaat gatattttgc tgttgcccaa taaatagatg gggtagtagc taatcccgca
    18841 tcataaatat cgcatccaga gcacatcaaa ccttctatta aagtatcgcg aattgcatcc
    18901 gaagattttc taacatctct acctatcaat atttttttaa cattaaaaat ttgtggaaaa
    18961 taataaccta tttttaatac atcttcatta ttaaaatctt tgccgtaaat acccctaatg
    19021 tcataagcat gaaaagctcc catatttttt tttgacaaat atacaataaa tttaatgaaa
    19081 aggttgaaaa aaatgattta aaatatgcgt ttgtctaata tggacaaaat agtaaaataa
    19141 atgtagtaaa agtcatcctt gctaactggt attttattta aacaacgaga atattgacta
    19201 tatgttatta actgaatgcc tattttgata agtttaaata atttaaattt ttttaaaatt
    19261 tcaataatat tattatcttc gtaaaacatt tacatgagac agctcaatat ttgtattttt
    19321 ttgtctttta tacatttaac tctattttca caaatatctt ttactgattt agatagatta
    19381 acaagaataa cgaaagatgt gaaagcactc tctcatgata ctatgatggg gcgaaaatca
    19441 gctacaaaat atgagtggaa agctggcaat tatattattt cagaattaaa caaaatatct
    19501 gtccaaaaat tacctggata tgaatctttt aggttggcat ttactataaa caatgataaa
    19561 ataaaaagag atacgacagc tgacataata gcatannnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19681 nnnnnnnnnn nnnnnacagc tgacataata gcatatattg acaatggtgc cccatataca
    19741 ataatattgg gagcacacta cgatcatata ggatatgaag tgtatgatat tactaagaat
    19801 caatatttga tatataatgg tgctgatgat aatgctagtg gtgtagcctg tttattagaa
    19861 ttagctagac atttatccac tggattttta ccacaacata attatattat agctttttgg
    19921 ggaagtgaag agataggatt gtatggctct acatattttt gtcaaaaaat attaattaat
    19981 ttgaaagtgc cacctataat tttatatata aattatgata tggtaggtag tctaggctat
    20041 aaaaaaaacg aattaatatt atatggatca gctacaggta aaaatataaa aaaatatata
    20101 ccacggaaat atgatacagt aaaaattata aaattgccag tatcattttc atttagtgat
    20161 catacttgtt tttataaaaa caaaatacct tatttatatt ttacaactgg tttacctaaa
    20221 gtttatcata ctataaaaga tgagtttaga ttaataaatt ttaatggcat tttattcaca
    20281 ttaaaactaa cagaatacat tttagaaaca atcgaaatag aatctataag atataataaa
    20341 gcttgcagtt taaactatat aattacaggt gttaaattat taatgtcaaa atgacatata
    20401 aatttgtttg gcacaaaaaa tgataaaatt aaagcaaacc aacaaaaaaa ctaaagatta
    20461 tgagtgaaat taaaattaaa cctttagcag atcgagtagt tatcgagcca gcacctgctg
    20521 aggaaaaaac tgcaggtgga attataattc cagatacagc gaaagaaaaa cctttaaaag
    20581 gtacagtagt agctgtagga ccaggtaaaa aagatgaacc aatgacagtg aaagcaggag
    20641 atgtagtctt gtattctaag tatgccggaa cagagatcac cattgatggc aaagaatact
    20701 tgatagttaa agaatccgac attttagcaa ttatttaata attaacatta aaaaaaataa
    20761 aaactatggc aaaagaaatc atattaggct atgaagcccg agagcaatta aaaaaaggcg
    20821 tagataaatt agcaaatgct gtgaaaataa ccttgggtcc aagaggacgc aatgtagttt
    20881 tagaaagaag ctatggagca cctgtaagta caaaagatgg tgtatcagta gccaaagaaa
    20941 taagtttaga agataaattc gaaaatatgg gtgctcaaat ggtgaaaaaa gctgcgagcg
    21001 aagctagtga cgcagctgga gatggtacca caacagcaac tttgttaact caaatcattt
    21061 ataatgcagg actgaaaagc gtaactgcag gagctaatcc tatggaccta aaacgtggta
    21121 tcgataaagc tgtaaaagca gtgatagaac acattaaaca gcaatctcaa cagataggag
    21181 atagctatga aaaaatcgaa caagttgcta ctatttcggc taataatgat cgtgaaatag
    21241 gtaaaactat tgcagaagca atgcgtaaag tcaagaaaga aggtgttata accatagaag
    21301 aggccaaagg cactgaaaca actgttgata tagtagaagg tatgcaattt gatcgaggtt
    21361 acatttctcc atattttgta actaattccg aaaaaatgat tgtagaatat gaaaatcctt
    21421 acattttact ttatgaaaat aaactaagtg ggatgaaaga gctacttccc atattagaaa
    21481 aagttttcca aactaataga ccacttataa taattgctga agatgtagaa ggtgaagcat
    21541 tagctacatt ggtagttaat aaattaagag gttcattaaa agtagcagct gtgaaagctc
    21601 ctgcttttgg tgatagaaga aaagaaatgc ttgaagatat cgcaacattg acaggcggca
    21661 cagttattag tgaagaaaaa ggattcaaat tagaagatgc tacgctcgat ctattaggtc
    21721 aagcaaaaaa agtaactatc gacaaagaca atactactat agtagaaggt gctggtagta
    21781 aagaggcaat agaagcacgc atcaaacaaa taaaagtgca aatagaagat gctactagcg
    21841 attatgataa agaaaaactt caagaaagac ttgctaaact tgctggaggc gtcgctgtta
    21901 ttagagtagg tgctgctact gaggtagaac taaaagagaa aaaagataga tatgatgatg
    21961 cactgcatgc tactcgtgca gcagtagaag aaggcatcat acctggaggt ggcgttgcat
    22021 acataagagc tatcgaagcg cttgataata ttgatgtttt caatgaagat gaaaaaatag
    22081 gtgttaatat tattcgtaga gcattagaag agccacttcg tcaaatacta gaaaatgctg
    22141 gtctagaagg cagcgtaatt gtacaaaaag taaaagaagg taaaaatgat tttggtttca
    22201 atgcatatac tgaaaaattt gaaaatctat tagaaactgg cgtgatagat cctactaaag
    22261 tatctcgtgt ggctttagaa aaagcagcat ctattgcagg ttctttacta attacagaag
    22321 cagctgttgc tgaaatacct gaagaaactc cagcagctcc agctatgcca ccaatggact
    22381 attaaaatag tatttgataa atcaaataaa agggtcacat taatgtgacc cttttatttt
    22441 tagattaaat tcaaagcctt gtgaatattt aataaggttt ttataatctt taataaatgt
    22501 ttaagttttt caagtttata tgttaaataa tttttatatt tttataaaaa ttattattca
    22561 tgaaaaaaat tgtagaaaac accagtaaat accttgctat gagtaaggaa gagaataaag
    22621 aattcttaga caacctgtat tctctaatga aaaaatgatt ttggaatata gccctacact
    22681 attgtttgta gagcatgatg atgccttttg taagaatatt tatacgaaaa atattaattt
    22741 gaacggaggg attacatggt aagtaaaact gatgccattg agataataac atacgctgaa
    22801 gaaaatggaa ttaacatatg gattgatggc ggttggggag tagatgcatt attagaagaa
    22861 gaaacaagag tacacaacga tattgattta tttgtggaag aaagaaatag taaaaagttt
    22921 attgaagtaa taaaagaaaa aggttttgcg gaagtaacag aagcatatac caccacatct
    22981 cacacagttt ggaaggataa taaaggtagg ataattgatc ttcatatatt taaattcaac
    23041 gaacaaggat acattgtttt tgaaggagaa gcatatcccc caaaagtgtt tagtggtatt
    23101 gggaaaatag gcgataaaat ggttagatgt attgatgctg aaaatcaagt attatttcat
    23161 ctgggctatg agcatgatga aaatgatatt catgatgtaa aactattgtg ccagagattt
    23221 gatattcctg ttccgagtga gtacaagtca gtttaacaat ataaatttat tggacatgtt
    23281 cccgcctacc gatagcccca aaaacgcaat ggatatgttt ccgagaacgg gcatactcaa
    23341 atgacattca ttctgccctc tcgagccacg ttaagtgcgt ggtaatgatg tccacgcaca
    23401 aaaaattaat ttttccgaaa acccttatat atcaatgctt ttagcacaca tggatacaaa
    23461 acccattaag tcaataacat gattttttcg gttcgaggaa acaagtccat aaaggcggtt
    23521 tttgaggttg agtggtcagg ttggatgttg ggataagtaa actgataata tgtgggcgat
    23581 aaatcaagaa tatacctagc agactgtttc cgtatagatg gagagactgc ttggttttat
    23641 attgagtata aactttaaac ttttttaaaa tactttgcag ccttttcgtc gtataaagcg
    23701 tatataggtt gaaggaggtg aagcaaaagc caaagaaagt agccgtatta gctgctactg
    23761 acagagctgg caacttgttg tttgaaaaat ttgaaggcaa aaagctaagt tcagaacaga
    23821 tagaacaatt tctgaaggct aagatgccaa aggatggagt catgtgtagt agtaataaaa
    23881 aagcattcaa aaaggttaga agaaatgtaa tcaagcataa agaaatagta accagccata
    23941 gacaaaagcg aggtatatat agcctaagta ccgtcaagaa aaaggctagt gaattcacta
    24001 attggatata tttcaaattt aggggtgtag ctaccaaata cctgcaaaac tacattatgt
    24061 ggtatgtagt gatgggtaaa tacctcacag gcaagactgt aagcaatact gggcgaatgc
    24121 tcaatcttgc atcctcggac aggtgggcat ggtatcggta tagagaatta gtgaatataa
    24181 catatgttaa ttgaaatata tattaattaa ccaaattcgc aacacactta tccctgtata
    24241 tattttaaaa caattaaaaa aattgattaa ttaaattatg aattcattta ctcatttaaa
    24301 aaataatata ttacttagat caaaagttgc ttttataatg caaataaagt atttatttaa
    24361 acaaatgagt ctattttgat ttatatttat gatttttaat tatttttaaa aaattatttt
    24421 taaaaaatta tttatttttt aacattatta ttataatttt gtaaaaaaat gagtgaatat
    24481 ttacctcatg aatgcggaat agttttactc cgtttattac agcctctcag ttattatatg
    24541 gagaaatata actgttgtta tcatggtcta gatatgttat atttgttaat ggaaaaacaa
    24601 cataatagag gtcaagatgg tgcgggagta gtatcagtta agttagatgt agagcctgga
    24661 tatagatatt tagataggat acgctctaat gttgattctc ctatcataga tatttttcaa
    24721 aaaatttatg atcctttacg catattagaa gccaacaatt cgcctttact ctatgatcct
    24781 gaatatctta agcgaaatat accttttatt agtgagttgt atttggggca tcttagatat
    24841 ggtacttatg gtaaatatca aatagaatat gtacatccac atctcaggca gagtaactgg
    24901 cgtacgcgta ctttagcctt agtgggtaat tttaatctta ctaatgctga tcaacttttt
    24961 ttagatctta taaattatgg acagcatcct atagagactt cggacactgt tactattctt
    25021 gaaaaattag gatattattt agatgaaatg aataacgaat tgtatgaaaa gtttaaaact
    25081 cagggttata ccaaagctga aatttctccc aaaatagcag aaacattaga cattgttagt
    25141 atagttaaaa aagcagcaca aaactttgat ggtggttatg ttatagcagg agtactaggt
    25201 catggagatg cttttgtatt tcgagatcca cgaggcatca gaccagctta ttattataaa
    25261 gatgatgagt tttttgtggt tacttcagag agaccaccaa tacaaactgc atttaacgta
    25321 aaaactgatg atataaaaga aatgcctccg ggacatgttt tgattttaaa aaaaaataaa
    25381 gatttatcta ttgttcccta tacagataat ataccttcta aaatggcttg tagttttgaa
    25441 catatttatt ttagccgtgg aacagataga gacatatata aagaaagaaa aatgttaggt
    25501 gctatgctgg cagatcctat attatctgcc attgactatg atttagaaaa tacagttttt
    25561 acttatatcc ctaatacttc tacagtagct tttcttggtt tatatgctga attagagaaa
    25621 tggcttgatg tacaaaaaaa agaaaaaata atagatatca ttaatatggg ggaaaatatt
    25681 aatgataaag aattacgagc aatacttaac tggcatccgc gtgctgatac actcgtagta
    25741 aaggatttga aacatcgtac ttttatcact aaagaatcta aacgcaatgg attagctagc
    25801 catgtgtatg atgtgactta tggtgtagtg agacctaata ttgatagaat tgtagctata
    25861 gatgatagta ttgtacgtgg taccactata aaaaaaagta taattccaat gctaaatagg
    25921 ttggaaccaa aagaaatcgt aattgcttct acagctcccc aaattaggta tcctgactgc
    25981 tatggtatag acatggctcg tttagaagaa ttcatagctt tccaggctgc tatttctctt
    26041 ttaaaagaaa gaggacaagc caaattgata aaatatgtat acgataaatg taaagagcaa
    26101 gattcattac ctgacaataa attagaaaat tacgtttctc ttatttatca accttttgaa
    26161 gaagaagaaa taactcataa aatagaagaa ttattaactc ctatagctaa taaaactaag
    26221 ttaactatca tttatcaatc cttagaaggt ttaagtaagg ttttgcaaga caataaaggt
    26281 gattggtatt tcactggtaa ttttcccact ataggtggat atcgagtggt taataatgct
    26341 tttattaatt actatgaagg taaaaataaa agggcatatt gaaattaaat tatttataat
    26401 tgataattat gataaattta aagtctcaaa aaaaaagtga tgagtagaaa ccattagaat
    26461 acattttggt aaatttatta tttttgacta atttattttt atattctatg tataactaat
    26521 taactatttc tttaattgtg ttaatgttta attcccaatt atcaaaattt ccactactca
    26581 tcaaaacaat agctgcaggt aatttaatat attgctctat aatattttga atatcattta
    26641 ctatggtggt aacaaataga ttttgctggt caaaggcttt tattatgtct attgtattta
    26701 gatctggcaa ttgctttatc tctagggcat gtttactata aaaaactatt gagatatctg
    26761 aattttgcat agttttacta tactgaggta aaaaattttg gtttaagcta gaataagtat
    26821 gtaattcaaa tacagagatt agctgcctat caggatagtg ttctcttacc gcatcaatgg
    26881 tagctctcac ttttgatggt gcatgtgcaa aatctctaaa aaccaccaaa tttttatttt
    26941 catataattt ttctaacctc ttattagcag aacgaaagct catcattgct tcacaaaaat
    27001 ctttggtatc gatatctaaa tattcacaaa ttttatgtgc tgcagatata ttttgcatgt
    27061 tatgcttacc aaaaatttgt aagggatatg atttttcttg atatttaaga aaaaaatgcc
    27121 cattttctac aaagtatgga aaggttttat aaaaaatggg agttagtttt agttgtgctt
    27181 catccaatat tttgcgtact ataggttcgt ccactgcaaa taaagcacca tcagaaggaa
    27241 ccatttcggc aaatttttta aattgctcta aataattttc aaaactagga aaaacattag
    27301 catgatccca gtctatacca tttatcaatc ctacatctgg tttataaata ataaattttg
    27361 gaatagggag ctcagtacta gctaaatatt catcaccttc tatgatcatt ataggagctt
    27421 cattacttag ttttatgtta aaatcgaaac caaccacctt agaacctact aaataatcga
    27481 attgcttgtt taagagtctt aatacatgca ttatcatacc tgtaacagtt gttttgccat
    27541 ggctgccacc tatcactatc ctatgtttgt ttttcgaatg ttgataaatg aattctggca
    27601 tagataaaac gtctaggttc aatttttttg cttcagttaa ttcaggatta ttagccttag
    27661 catgcatgcc agaaattatt aaatctatat ctggagtgat tctatcagcg aaaaaaccaa
    27721 tttcacatgg caatagatta tatttttcta aattttcttt tgctggattg aatatttcat
    27781 catctgatcc gctaactatg tgtccagcct gttgtaaagc tattgctaac tgatgcatta
    27841 cagcacctcc tatagctatt atatgtattt tcatgattga ttatacttac aataaagaaa
    27901 ttttaattca caaaattaca aatttatgca taaaatattc ccctttatct gttttaaaag
    27961 aaaagattag taataatcaa attttttttt aaaagttata attttgcaac tattaataaa
    28021 aattatttct cttatttgaa tatttgtttt ttaaatttta gtattattat aattctatag
    28081 ctctataaat ataacttaat agaaaggtta caaaaatttt tataatagca ataaattcat
    28141 tatttttgta taaaataaat tcaataaaaa ttatcatgct taaacaaaca caacatttaa
    28201 gattattaca acgactatct ccacagcaga ttcaattgat gaaattgata cagctaccta
    28261 ttgctgagtt agaacagcga atcaaagaag aattaatatc aaaccctgct ctagatacct
    28321 tagatgatac tgatgacgat gagaatataa tgattattga tgatgaaata gataaaaaca
    28381 atgaattgga ttatccagac atagacgatg aaaaaacaga tataataaat gatgatgatg
    28441 aattattaga tgaatacata gatgaagatg aaattcctga atataaatta tatataaata
    28501 atactagccc tgatgactct acaaacatac aatattatga ctatactact aaaacattaa
    28561 atgactatct aaaagaacag ctagattata aaaatctaag tgagcaagaa tatttgatag
    28621 gagaatatat aataggaaat attgatgata atggttactt acaaagaagt atagatgcca
    28681 ttgtcaatga tttattattt ataaggggaa tcaatacaaa cgcagatgag gtagaaaaag
    28741 tgctcgacat aatacaacga ttcgatccac caggtatagg agctagagat ttaaaagaat
    28801 gtttactatt acaattagaa gcaaaagaac ataaaacacc ggaaataaac ttagctattg
    28861 aaattttaga aaactattat gatgatttta ttaaaaaaca ttatgatcaa ataatcaaaa
    28921 aagctaatat cactgaagat caattaaaga tggctataag tgagatacaa aaactaaatc
    28981 caaagcctgg taattctttg tatcaaattg gcaattcagc tataagtata ataccagatt
    29041 tcatcatata taataatgat ggagaacttg aactgacatt aaataattac aatattccat
    29101 acttatgcac aaataaaaaa tacgaaaaca tgtatcagag tctgtctaaa gaagctagta
    29161 atgatcctaa gaaaaaagaa actgttcaat ttattaaaca aaaactggac ggagcacgtt
    29221 ggtttatcaa tgcacttgag caaagacaaa atacccttta tataactatg aaaactatta
    29281 tggaattgca aaaagatttt ttcatgactg gcgatgaaac taaactaaag cctatgatac
    29341 taaaagatat agcagataaa ataaatatgg atatttctac tgttagtaga gtggctaata
    29401 gtaagtatgt ggctacaccg tatggaacat ttcttttaaa gcattttttc tctgattcat
    29461 tagaaactaa atcaggaaaa gaagtgtcga ataaaactat aaaagcaata ataaaagata
    29521 taatcaaaaa tgaagataaa tcgaatccat taactgatga ggaattgaca aaaatattag
    29581 aagaaaaagg gttcaaaatt gcacgaagaa cagtagccaa atatagagat caattaaata
    29641 tacccgttgc cagacttaga aaagaactat aaaataatta acatcaaata tgaaaaaaaa
    29701 tctatctcag gtaatatcta tagctttaca tcctgttttt atccctatat ggtttgctat
    29761 tatcttattt aattctggtt atttcttaaa tgctttttta ccaaatgctt tttttaaatc
    29821 atatatttgg ttgcttatca tcattatggt gatcatcccc actatcatta ttcttttttc
    29881 gtatcattta ggattgattg aaagcgttca tagctctata cctattgagc gaataaaaat
    29941 attattgata attttagttt ctagttttat tttatacttt tttttaaaaa gactacatat
    30001 acctatattt tatttattac ccataaggct tacaataatt ttaacgactt tgttatgtat
    30061 ttttagtagc tttatgaatg ttagcataca cagtgctgga tggatgaatt tatttacttc
    30121 tttatatata ttgcaatata gacttataga aataaatata ttttggatta ttgttattat
    30181 acctatattg tggggcttgg ctgcccaagc taggtatgta gttggcaaac ataatagatt
    30241 acaaatattt atgggggctt ttttaggaat aataactggt ttgagtgtct ttatggtttg
    30301 attagttggt aaatatttta atatctacgg ataatatggt ttttatgtaa ttttgtaatt
    30361 aattattttt gaattttatg aattttatct tatatctaat aattggttta ttacttttat
    30421 tttggatcac acaaaggttg ctgccctatt ttttaaaaag aaaattgaaa aaataccaag
    30481 ataaaatgaa tactcataca gaaaccaaaa aaagtaaaaa tataaaaata aaaatgccta
    30541 aaggttataa aaaaagcaaa gtagatatat ctgatataga agagataaaa tataaagaaa
    30601 ataaaaaatg aatatcttaa tagtagcata ttattggccg ccagctggtg gtagtggagt
    30661 tcagagatgg ttgtacatgt ctaatcattt agcggatatt ccaggtaata atctaaccat
    30721 ttatgtgcca gatactacag attatcaagt gttagatcct actttatctg ctgatatttc
    30781 accaaaattg aaaatcatca aaagacctat ttgggatcca gcacattttt ataataaagc
    30841 tgtaggtgag aaaaacaaaa taggtgtcgg gatgagctct gtaaaagaaa aaaaatcatt
    30901 gattcaaaaa ataggattat ggtttagagc caatgttttt gtcccagatt ctagagtgtt
    30961 ttgggtaaat ccttcatatc gttttttaaa aaaatatatc gaaaaaaata aaatagacgt
    31021 tatagtcact actggtccac cacatagtat gcacttgata ggactcaaac taaagcaata
    31081 tttcaaagaa aaaattattt gggtagcaga tttcagagat ccatggtcag aaattcaata
    31141 ctatgaaaca ttgaaaacta caaagtttgc atttaataaa ataaaaaaat tggaaaaaaa
    31201 agttctaata aattctgata ttgtaactac tgttacccct tcatgggcta atatgtttcg
    31261 agatatttct aaacgaaaaa ttgaaataat agaaaatgga tatgaggatt cattatttga
    31321 acatataaaa atcataaatc aacctaaaag atttaatatt gtacatacag gcgtaatgta
    31381 tgctacgaga aatccacata atttatggaa tagtttaaga attattaaaa atgaaattcc
    31441 cgcatttgtt gatcttgcca aaattcaact tataggaaat ttggatgcta gtgtaatcaa
    31501 tgaattaaaa aatttggatc tttatgatat aaccgaaata tatccaccca tgtctcataa
    31561 ggaagttatt gaatatcaaa taaatgcagc actccttctt ttaataattg atcaggcaaa
    31621 aacatatatt tcaactgcaa aaggcatgat cactgggaag atttatgaat attttgcatg
    31681 tcaaagacct atcctaggca ttggcccaac tgatggtgat gcagctagga ttttaggtga
    31741 ttcagaatat gcaaagatgg tagattggga cgatttagaa ggtatcaaaa attttattac
    31801 aaacatttat caaaaatata ttaatggtga taaattagta gtagattatc atcaaaaaga
    31861 attatacagt agaaaaaatc ttgctcttaa atttaataat attttgctcg aatatattaa
    31921 taataaaaaa aataatggat aaattaattg atttctgata cattagtatt ggctaaattt
    31981 tgatccgtta taactataaa aatttatcaa atctagattg ttcgttgtta tttctccata
    32041 gatagacatt aattagtaca aataaagata aaagttggat agttgagaaa agtttagcaa
    32101 aacagttgtt gccagttgtt agctatttat aaatcacaat tacatggtgt tgaaaaatat
    32161 atttttgtat taggtagata acgctctcgc acctcttttt gatgttttaa tggtatcata
    32221 tttaatctta aatttaatac tttcaatgtg ttattcaatc ctgtaatact ctgattgagt
    32281 tttgttaaat aattatcacc taagtctaat ttttctaaac aacaaaattc ttttatctca
    32341 tttggcaaat attcgatttc attatgatac aaattcaatt cttttaaatt tggtagctct
    32401 atcaaaataa ttgggaaatc tttgaatttg ttataactaa gatctatttt tattattttt
    32461 ttacaattaa taatgttctc tggtaaacta tttaatttat tacgctttaa taccaatact
    32521 tctaaatttt gaaatgtatc tatttttgat gttaattttt ctaatttttt attttttaat
    32581 attaatactt tcaccttttc agcatcgcta agggcagtgt ttaaatcttt atatattcct
    32641 tcattttgtc cttgtaattt aaagaaaatt aaaaatataa taaaagtata taaaaaaatt
    32701 ttgcacataa attaaaaatt acatatccta ctacaaaatt aaaaataaat aactataatt
    32761 tttataaata ttttttaata atctatctgg ctttttattg tccatcagtt tcttatctca
    32821 ttctatcgtc caccattaga tttctccatt attctaaagg taaaaccttg ccactgcctt
    32881 tataattgaa atttttttct gatggatttc ctctaaaata tacattgcca ttgctattaa
    32941 tatctaccca caaaacatta ttaacacaaa catatatatt ttgataacta caagaattta
    33001 caaacacagt atctgaacat agatattcta aatgctgtgg ccctgcaccg tgtagatatg
    33061 tataaagata tttactttgc ccagtaatat ataatcccac attgccaccg tgagatctaa
    33121 agataaagcg atttacattt aattctcctt ttatatctaa agctgagtcc catacttcta
    33181 tatttattgt gtcctgtata caagtatttt cgaaaatcat ttctccataa atctcaggat
    33241 ttatataatt aatatttcta taatgtgttt ctatatctat ccaatgctta taatttctaa
    33301 gccaataaca tttgtttttg tctttaattt ttaattcata atttttttga tttatagata
    33361 ctccatttat taaattttcg ccacctttga tttttaaaaa atctatacta tcttttatta
    33421 aatataaatt tactctaccc tctacatata gtctatctat gctatctaaa gggattataa
    33481 tttccgaata tttacctact gacatcaaac aagattcttg tttttcgcaa gaaaaaaata
    33541 aaataatcaa agcaattaaa ataattattt tcctctccat actaaccccc attctattac
    33601 atcagctttc acataatgac tacgcaaaga aatctgtgcg ccaaaattat ttgaaaaaaa
    33661 ataacgtata ccaaatctag tataaaatgg ttgatatttg aaagctaaat ttctcaaata
    33721 tatgccaact tgaaaaatta aatccatctt atctatatgt acatcccatc caccttttaa
    33781 tgaaatttta agtggtatag cattatattt atctaaatcg aagtctttgg tttttaaata
    33841 atctggtaaa gatccatccc aaataaaatc tgtacctacc atccattgta ccgcgttata
    33901 atgcgataca taatattcga aactagtagt gtaaacagaa tagtagacgt cattagttct
    33961 attttgtttg atagaatacg tcccaaaaga gtgatatttt ggttgaaaca aattaaaagt
    34021 atcaattttt ttatttttat tatgtttttc ttgaaaatat ccaatagaaa ggtgtagact
    34081 tgggatattt aatcctgtat tcggcatttt aatagctcca ttagaaaaat gattccatct
    34141 aaactgcata tttattagca aaggactctt ttgataaata ataaaatccc atccaaaatt
    34201 aatatgtaca ttgaaatgtg acccaatagc aaaattgcga taattatctt cagtataatg
    34261 cttggataaa tatgacacgc cactacctag aaaaaaatat attttttctt tataaactct
    34321 aaaatatata tctcctaata aactataagc ttgcccgagc actttattat tactcaagct
    34381 tgtataatat gcttctattc tgtatctttt aggactttga aaagtatcta tagggtaata
    34441 cttattttca taaaataagc taaaaccagc tgggtggttt tctataaaat tatacatttt
    34501 tttgtaatgt ggaaataaaa aaccataact ccctgatata ccaatactta attgagagaa
    34561 ggctaatata ggaataaaat atatgaataa aattattttt ttcatcagaa aacaaaaata
    34621 taaataatat gaataatgaa atcctttgaa ggtaattgaa aaattgtctt ttaagctgat
    34681 atttagctag atagtatcaa tttaataacc ctaaatctct cagaaatttg cattattcag
    34741 tttttttttt aaataaaaaa ttaatatttc aggatataac tcgattaatt ttcatttact
    34801 taattgcaat ttttttttat ctttgtaaat aaaaaaaatg aaaagattat caattttttt
    34861 attgttaaat ttaattttaa tcaaaatgag ttattcacaa caagaaacac gtttattacg
    34921 ttttcctact actgatggga gtaaaattgt tttcacgtat gcaggacaat tgtatactgt
    34981 gtctaaagat gggggtatag ctcgcgagtt aactacaggt gatggttatg caatttttcc
    35041 tagattttcg cctgacggta aatggatagc attcactgga caatatgatg gcaatactga
    35101 agtatatttg atgccagccg aagggggcat tcctaaaaga ctcacttaca ctgctactct
    35161 caatcgtgat gatctaagcg atcgtatggg accaaataat atcgtaatcg gctggactcc
    35221 tgatagtaaa tacattattt atcgcagtag aaaaagttct tttaatgatt ttgtaggtca
    35281 attgtttata gtttctattg atggtggtct ctctactcaa ttaccattat cctctggtgg
    35341 cttctgctct tactcgcctg atagtaaata tttggctttt aatcgggtgt ttcgtgaatt
    35401 tagaacttgg aaatattatc gtggcggtat ggctgatgat attagaatct ttgatttcga
    35461 ctctaaaaat atcattaata ttactaataa tgaagctcaa gatataatac ctatgtggat
    35521 aggagaagag atttatttta tctcagatag agaccgcatc atgaatctat ttgtttataa
    35581 tactaaaact gaagaaacta aaaaagttac aaactttgat gattatgata tcaagtttcc
    35641 ttcacactca gtgactaatg gattaatagt attcgaaaaa ggcggttata tttatttatt
    35701 tgacactaaa actaaagaag ctaataaagt ttctattatc ataaatcaag atttaccttt
    35761 agctagaacg ctaaaagtaa atgctgctga ttatattaca tcttttacat taagtcccgg
    35821 tggagaacgt atgggtttct ctgcccgtgg cgatattttt agtgtaccag ctaccgaggg
    35881 cgttgttaga aaccttgcta aatcatctgg tgtgcatgaa agaaatgtag aatggtcccc
    35941 agatggtaaa tatttagctt atcttgcaga taatactggt gaatttgaaa tttatattca
    36001 aaaacatgat ggttcagaac cccccattca actgactaaa aatgcagata cttattattt
    36061 cgaaatactc tggtccccag atagcaaaaa attattgttt agtgacaaaa aacttcgcct
    36121 tcgttatgtt gatattgata ctaaaaaaat tactgaggtg gctcagagtc aagtatggga
    36181 atatcgtgat ttcacttgga gcccagacag tagatatata gcatacagcg atccacagaa
    36241 aaatgatttt aatattattc gaatttatga tacgtatact aaagaaactt ttgaccttac
    36301 agatatgtgg tacgactcac atagcccaca cttcagtcct gatggtaaat atctttattt
    36361 cgtttctgag agggatttta atcctattta ttcacgtgta gagtggaatt atgcttatca
    36421 ggatatatct aaagtttatc ttgctattct cgacaaatct atacctaatc cattagctcc
    36481 cagaaatgat gatttagatt taagtaaaaa aactgctgat gataaaaaat cagataaaaa
    36541 aaagaaagat aaagaaataa ctgaagagga tgcacttgta aaagttgtaa tagatattga
    36601 tggattaaaa gatcgtatca ttagcttgcc tatcgaggta tctacatatt ggaatctaca
    36661 agctactaat gataaaatac tttatcagag aagaggtatt aaagatgaga aaactcaatt
    36721 attagccttc gatttgaaag aaagaaaaga aactaaatta ggtgaatttg atacttatct
    36781 tttatctaat gatcagaaaa agattttaat taaaagtaaa aacaattact atgttttaga
    36841 ttttgctgtg gcagagctaa aaccagataa aaaagtcccc acccaagaaa tggtagtatg
    36901 ggtagacaaa aaagcagaat gggaacaaat attttacgaa gcttggcgac agatgcgaga
    36961 ttttttctat gtacctaata tgcatggcgt agattgggaa aaaataaaaa ataaatatga
    37021 agttttgtta ccttatgttg ctaataaaca tgatttaaat tatattattg gtgagatggt
    37081 tggagagcta agtgtggggc atgcttatat aggaggtggc gatttaccta ctattaataa
    37141 aatttacact ggccttttag gtgcagaatt atcaaaagat actcaaagtg gttattttaa
    37201 aatagataaa atattagaag gtgccaattg gagcagagac cttcgtagcc ctctacaaga
    37261 gctacaggta aatgctaaag tcgatgatta cattatcagt atagataata tacagactaa
    37321 tacagtgaat gacatttaca agctattgat agataaagct aataaacaag tagagatagt
    37381 aataaatgat aagccaaata atgaaggtgc tcgcaaagtc atcgtagtac ctgtggctaa
    37441 tgaggcaact ctatattact ataattgggt gcaaaataat attaaactgg taaatgaaaa
    37501 aacaaatgga caagtaggct atattcacat accagatatg ggtgtagatg ggttgaatga
    37561 gtttgttaaa tatttttatc cccaattaac aaaaaaagct ttgataatag atgatcgtgg
    37621 taatggtggc ggtaacgtat ctccaatgat tttagaaaga cttctcagag taccacaacg
    37681 tgctacaatg gcaagaaatg tattgattcc aggagtagtg ccaactcaga ctatggtagg
    37741 acctaaggtt ttacttttca atcaatacag cgctagtgat ggagaccttt ttccttatgg
    37801 attcaaaaaa tataatattg gcaaaactat tggcgtgaga tcttggggcg gtgtaatagg
    37861 tatacgtggt tcattacctt ttgtagatgg tacttatctc aataaaccag aatttgctag
    37921 ctatagcata gatgagtcta aatggattat agagggttat ggagtagagc cagatattgt
    37981 gatagataat gatccatata gagaatattt aggtatagat gatcaactct taaaagctat
    38041 tgaagtgatc atggaagaac tcaaaaatgc taaagaaatt cctgatatac caactcctcc
    38101 cgacaaatct aagtaatcga agattgttaa aatagatatc tgaacaattg attttgtttc
    38161 attaataagg gcatattttg ctatgtattg atataaaata ctctttttat ttgcttcata
    38221 cttttctcgt cttttatact caatttcaca tctgacaccc ttctatattt cactgttatt
    38281 aattttatat tgtatttata aataaccttt ataaataact ttacaatatt tattaataat
    38341 tgatgaatat tctaattttt tatttttatt atcaaaaatt gcaaaaattg ctgatccact
    38401 accactcaga ttaacataaa ttgcacctaa ttcatataat ttttctatta attcatacaa
    38461 aaaaggatac ttattaataa aaatttccaa aaaatcattt tttagctgat atttccactg
    38521 aaccattgga taatttttga ttattgtttt tatatcttct ctaaattcat taggttttat
    38581 ttgagaatat gcttcggttg tagatacttg aataggttta tcattcaaat aaggcaaaaa
    38641 aatatatata tatttatttt ctaatgataa atttatggtg gtcagaatat caccttgttt
    38701 ttcagcaatt agaggattgg catagacgaa aaaaggacaa tcactaccaa gcttgataga
    38761 aaattttagt aactcattag catctaaccc taaattaaat aatttattca aagcttttaa
    38821 tgtagctaca gcatcacttg aaccaccacc tagtccggct tgatatggta tgtttttttc
    38881 taaaacaatt ttaactggag gcaaattaaa tttatttttt aataaattgt atgctttaaa
    38941 aactaaattg tcttcatttt ctagttcgat atgatttggt acggttaatg ataatgtctc
    39001 tgaaatagag atagttatta tatcataaaa atcattaata ggatacatta tagtgctaat
    39061 gtcgtgataa ccattatcac gtttttttaa aacaaaaagc cccaaattaa tctttgctgg
    39121 ggctttttct ataattattt tgtctttcaa tattaaatta atctaaaata tttaaagatt
    39181 tgagatcgtc gaaagctatt ttcactcttt ctctcataga tatttcacct tctctcagcc
    39241 atgcgcgagg atcataatat tttttattag gtttatctgg accttctgga ttacctattt
    39301 gtccctgtaa ataatccttg tatttcaaat aatatttatg cacaccatcc caaaaagccc
    39361 attgtgtatc agtatcgata ttcattttta ctacaccata gcttacagct tctcttattt
    39421 cttctaaagc tgatccacta ccaccatgaa aaacgaaact tactggttta ggttcagaaa
    39481 tacctaattt tttgcatata tattcctgac tattttttaa aattacaggt ttcaatacta
    39541 ctgcacctgg tttatataca ccatgtacat taccaaaagc agcagctatt gtaaaattag
    39601 gactgatatt tttgagttct tcataagcat aagctacatg atctggttga gtatacaatt
    39661 taggattatc aatagcagaa tgataaacgc catcttcttc gcctcctgtt actcctaatt
    39721 ctatttctaa aaacattcct attttatgca tgcgttctag aaatgattta gaaatactaa
    39781 tattttcttc tatagtttct tcgctcagat ctagcatatg agatgaaaat aatggtttac
    39841 ctttttcttt gaaatatttt tcaccagctt ttagcatacc gtctacccag ggtaataatt
    39901 ttttctgagc atgatctgta tgtatcacaa caggcacacc ataatattca gctacattat
    39961 gtatatgcat agcacctgag atagctccta ttatagctgc ttcataattt tcatttgaca
    40021 atcctttgcc agcaaaaaat tgtgctcccc cactagaaaa ttgtattatt acaggtgagt
    40081 gcacatctct cgcagcttct aatactgcat ttacagaatt agtaccgact acatttacag
    40141 caggtaacgc atattcacgc tttttagcat cttcatataa tgcttttaag gcatctcccc
    40201 aaactattcc tggttttaat tgatttttta tactcataac tttagttttt tattttgcca
    40261 aaattaataa aaatttatat gatttacaat attattatat cttgatctat tattgattta
    40321 tttataaaat aggattttac ttttcattta gattataaaa tcttaaggaa tagataaact
    40381 tatcttaatg atatttgttt ttatcagaac tcgaaaaaat cattttgtat tattacaata
    40441 tttaagtaat tttgcagaaa aaagatatga aaactgtaag aatagcaatc aatggtttcg
    40501 gcagaattgg cagaaacgca ttcaaaattt tagaatctag taaagatgta gatatagtag
    40561 ctataaatga tttaacggca ccagctacat tagcacattt attaaaatat gattccatat
    40621 ttggcaaatt tgaaggtacg gttgatacta aggagcaaag tatatttgta aatggtaaag
    40681 aatataaaat ttatgctgaa aaagatccat cagttttgcc ttggaaagaa ttagatatag
    40741 atattgtgct agaatgtact ggcaaattta gaagcagaga tggaatgatg aagcatatcg
    40801 aagctggtgc aaaaaaagta attttaagtg cacctgctag caaaggtgaa gatgtagata
    40861 taactgtagt tttaggagtg aatgacgata aaataacaaa agatcatata ctcatatcta
    40921 atgcttcgtg cactacaaat tgtttagcac cagtagtaaa ggttttgcac gataattttg
    40981 gtattgaata tggctttatg aatactatac atgcctatac taatgatcaa attattttag
    41041 attcacctca taaagattta cgtagagcac gtgctgctgc tatgtctatt atacctactt
    41101 ctacaggagc agctaaagct attggacttg tgatacctga attagcaggc aaattaaatg
    41161 gtagctctat gagagtgcca gtaccagatg ggtcagttgt agatttctat gctttactaa
    41221 aaaaagatac ttctgtgaaa gaaataaatg ctataatgaa aaattatgcc gaaggtagct
    41281 taaaaggtat tttagaatac tgtgaggacc caatagtgag ctcagatatt atcggcaatc
    41341 cacactcttc tattttagat gcagaattaa ctaatatttc agaaaaaaga tttgtaagaa
    41401 tagtgtcttg gtatgataat gaatttggct attctaatag attagcagaa atcgctatca
    41461 aagttggaaa gatagaataa atagtttttt ctaataatgt tattaacatt tatcattaca
    41521 tttttgatat taatattagc tatactaggg ataggtatta aaatgttgat taaaaaagat
    41581 ggtaaatttg aaaaaaagtg ttatataata gatcctaaga caggaaaaaa aatatctact
    41641 tgtattcgat gtgacaaaga ataaaataaa aaatataatt ttcttatgag tttaaatatt
    41701 aaatttgaaa atatgcttga tgattttgag ccaggtgatg gttattccag aatagactat
    41761 tatgatgaag aaactactaa taaattggct gaatattact atgaaatcat aaaacttata
    41821 ggtgatgatc ctaatcgtga aggattgcaa aggacaccaa tcagagttgc taaagcaatg
    41881 caatttttaa tgcatggtta ttatgctgat cctgaacaaa ttttacgttc tgcaatgttt
    41941 aaagaagatt acagagaaat ggtaatagta aaagatatag aaatatattc tatgtgtgaa
    42001 catcatatgt taccattttt tggtaaggct catgtagctt atatacctta tggtaatatt
    42061 gtgggactga gtaaaatagc cagagtagtt gatgtatttg ctcgtcgact acaagtgcag
    42121 gagaggctga ctagtcaaat aaaagattgt atccaaaatg ttttacatcc tatgggtgta
    42181 gctgtagtaa tagaagctca tcacttatgt atgatgatgc gtggaataca aaaacaaaat
    42241 tctgtgatgg ttacctctga ttttacaggc gcatttagaa aagaagaaac tcgtgcagaa
    42301 tttttatctc ttattggtac aaaaacatta cattaattga aatcttataa aagagtaaaa
    42361 aatccaatat actttagctt tgatcaatag ttttatagct tagatagaag atagtctaat
    42421 tgaatattta tcaaaatctc agtctgaatg caaattatta taatattaat ttaagctttt
    42481 tttaaaaaat tattttgtta attaaaaaat tatttgtaat tttgcaaata taaatattgc
    42541 ggggtagagc agaggtagct cgtcgggctc ataacccgga ggtcgcaggt tcgattcctg
    42601 cccccgctac aaagctaaat ttaattcttt cgggcttttc acaacaaatt ttaattttgt
    42661 tttcacagtt tgattaggta atatatcata tctccaagtt actattttgg tatctggatc
    42721 tattttaccg ttacctactt ctatttcagt tacttctatt tctttttgct gtgatattgg
    42781 taattgatct tctattatga tttctattgg taattttttg ttatttctca caactatttc
    42841 ataggctctt atgtatttca ccttagatcc tatcaattgt ttagcagttg agtcttttat
    42901 gctattacgc gtgactatta catttttgtc tcttccgagt gacacttgca ttgtatcttc
    42961 tatttgatat gatgagattt tagtagtacc tacaaagctg ttctcacaat aaatattagc
    43021 tataccatct aaaaaataat atttatccca atcatatata tttgctatca aaaaagcatc
    43081 gggatctatc ttgggagccg tataatatag atattttgct ggcaaatcat aagattgtat
    43141 atttacaatt ttttcttgat tagatggcac atctatcttg gcttctaatg tgtattccat
    43201 cgatataggt gtctcatagc caatggccat catcactggt gctggtttgt tataagtttt
    43261 aatttcttct tctgcataag ctactgcaat attttccctt tgacccctaa tttcaatatc
    43321 cgatgttgct tttggcatgt aaacactaag ataccaagga tataattttg gtttttgttg
    43381 cgaaattgtt ggattagaag tgcttaaaat tagttttaca ttttcccaat tttcacctgt
    43441 agtttgaatt atttctcctt ttaaaaacaa tgaaatagga ttattaagat caacaaacct
    43501 aaggtcatag cttggtcgcc aattagcata attagtaaaa tatgaaactt ccaatctgcc
    43561 attagtagct ttatctgcat atagatttaa aagtatttca cctacaggac gtttagctat
    43621 tttttcgtat gatgctattt gattttgtaa cttattaata gcttctaata actctttgtt
    43681 atctctttga attgccaatg acctggtttt gatatctaac aatctcgaac gaaaaaattc
    43741 tgaagctttt attagttcgt ttacattggt tccattttgt gtgccaccta tagttttatt
    43801 agcttttatc attgcttctt cttcgatata tacttgtaaa atagtattat ttttatctaa
    43861 tttttgctgc aataattcta aggagtcctt taatatttta tatgtggggt ccttagcata
    43921 atcagataga taatttatag atacattcac agtttggatt ataaaatcac cttgaccttt
    43981 tacattaaca gttttaggat ctagatcatc tggcaaatta cttattaata ttttattatc
    44041 acctttttgt aaattgatat cagatattct agtaatctgt gcaccattta aaaatatttt
    44101 gatttcatta atattagaat ttacttttgt tatattttga gcaaataatg taaaaggtaa
    44161 taaaatgaat ataaaaacaa attttttcat aatattttaa tttttgtaaa attaatgaat
    44221 tttatcgtaa ttatattgag tttaaaaaaa tttaaaacaa tacaatttat tttgcttatt
    44281 tgtaaatttt aatttaaaat ttttattgga gtattatatt gtgcacaatg aaaataaccc
    44341 tcaatacatt gtaagagcta ttcacctatt accaaataat ataatgatcg tacaatgtaa
    44401 aaatcataaa ctcaaaattt cattgataat gtatagggca gtatctatgt tgccagatat
    44461 atctcagaaa gtagggatgc tttttattta tcctacgaca aatataatat ctaagtaaat
    44521 acgtgtatca atgatatcat ttgcataaat acttaaaaac atatataaag cattaaaaat
    44581 acaagagatt ttagatttta tttaaaattt gtttataatg ataaaataat taaatttgta
    44641 aaaaaattat gaagaatttt ttagttggaa tagatttttc taaaggttcg ataaaagcct
    44701 tagaatatgc agtaatgtta aacaaatata tagattctaa aatcactatg ctttatgtag
    44761 ataagcctat acctactgaa tcaatttatg ctacagttac agcaaactat agaagtgaat
    44821 tgcatcaaag atttgaacag ttattaaatg aatttagaca acatataaaa gaaccagaaa
    44881 atttagaata taaaatcact acaggtaaag tgtatgaaga gctagctaat tatgctaaaa
    44941 aaaataatgt agattatatt tttgcaggta cccacggcat ttctggttat gaggagttat
    45001 ggataggcag taatgctaat agaatagttt ctactgctcc ctgcccagta tttattgtta
    45061 gacaaacgta tgaagtacat gataaaataa atagtatttt gttgccaata gattctacag
    45121 ctgaaactac taataaattg ccatgggcta tagaaatagc taaaaatact ggagccgaaa
    45181 tacatatttt cggtgcatat attactgatt taactacttt gaaaaacaaa gtaaataaaa
    45241 atgtagctat gatagaaagc aaattagaaa cagaaaatat caattttaaa acatttaaaa
    45301 aaaatatatc taattatgct tctgatttga taacttatat cgaagaaaac aatatagatt
    45361 tggctatcat tatgacagaa caagaaaatc gaaatacaaa tattttacta ggacaatatg
    45421 ctatgcaaat gattaatcat tgtccagttc cagtattgag tattcatcaa gacgataaat
    45481 ataattttta attgaatcct ttatttctaa taagtaaaga agctactaaa ttaggtcgta
    45541 taaatagcct aaaaccaata tcagcatccg gttcggttcg tttttatgct attgtctatt
    45601 tcaatgatac tgcattatta ggttgctata acgataatgt aaaagaaaat gaagccttta
    45661 tttattttac taattttttt aataattaca atttgcctgt ccctaaaatt gttcatgtaa
    45721 acaaggataa aactatttat tttcaaactt ttatttctga tcaaactatc tatgataaat
    45781 tgaaatacaa caattacctt tttgatagta cgattattga ctattacaag caagctttaa
    45841 aattattaac taatttcaat aagcttacca atattgattt tagtaaaaca tttccgagac
    45901 attgttttga tagtagatgt ataagatggg atttgaatta tttcaaatat aattttttga
    45961 aaataataaa tattcctttt gatgaagata agcttgagaa tgatttcgat ttattagaaa
    46021 agaaaatttt gtctttccct caaaattatc tttcacttcg cgattatcaa tcttctaatt
    46081 tattaattaa agaaaataaa atttatgtga ttgattatca aggtgcaaga ttaggttctc
    46141 aaatgtatga tgttgcttct ataatttttg atagtaaagc gcgtatacct catgagatca
    46201 aattagaatt atttgattat tacttagatt tgatttcaat tgatgataat gataaaacaa
    46261 aattaaaaga agaattttat tggttagcac tttttagatt aatgcaagct tttggaacat
    46321 ttggttatag aggattaatt caaaataaac ctagttttac ctcttctatt atttatggat
    46381 ctaaaaatct tgaaattatt tataaatttt taaattttaa tgattattgc cagttaccta
    46441 taattattga aaatattatc aataaatacc aatccaatga taacttaata ggcaaaaaga
    46501 tattaaatat aaaaatttat tctttttcta taaaaaaaca ttacccacct ttagatatgg
    46561 aacacggtgg tggttttatt tttgattgtc gttgtattat taatcctggt attatacctg
    46621 aaatgcagag tctcaatgga aaggatcaat tagtgataca atatttaaaa acatctgaga
    46681 tcgtacaaaa ttttttaaat tatacgttta atacagtttt attagctact gaggaatact
    46741 tagctaaagg ttatacattt atgcaaatcg gctttggatg cactggtgga caacatagat
    46801 ctgtattttg tgctgaagaa atagctagaa gattaaaaga attttttggt gaaaaaataa
    46861 atatctatat aaaacattta gaattagaat aataaatgat ataaaaataa aacttattaa
    46921 tgcttcatca ataaaaatta tttcaaaaat ttgatttaca aattaattat aaattaaact
    46981 ctttacaatc aaaaataata attataaatg cgatgtgcag taagtagttt ttttttaatt
    47041 tttttgtaat taaaaaaaga ataatgcaat tttaaaaaat agcttaccat ctattgcttt
    47101 ctgtaaaaat caagcatttt cgcaattatt atactttcac tatgcttcgc cgtagaattt
    47161 agtatttatg ttgggcattt tatgttcata ttctattatt tcaccaaaac tattttcgta
    47221 acgttgaata ttgtcagata gagctcttag tagtttttta gcatgttgag gtgtacagat
    47281 aatgcgactt ttcactttag catcaggtac tccagggact atagataaga aatctaaaac
    47341 aaactcatct gtcgaatgag aaatgatagc taaattagaa taaacaccat cagcaatctc
    47401 aggaggtaaa actacattaa tttgattttc ttttttatct tttttatctt tttcattcat
    47461 aatttattga ttttgaaatt cttttttaga ctctattaag gcttcgtatt catccattga
    47521 accgactatt aagttttcat actggcgaat acctgtacct gcaggtatca aatgtcctac
    47581 gattacattt tcttttaagc cttgtaaagt atcacgagtt gcttttacag aggcattagt
    47641 aaggactttt acagtttctt ggaatgaagc agctgataaa acactttgag tttgtagtgc
    47701 agctctagta atggattgta atacttgttt agctgtagct ggttgagcat cacgagcttg
    47761 tatgagattc atatctctac gttttaaaac agagttaata tctctcaatt gtctagcagt
    47821 tataagcata ccaggacgta aatcactgtc accagcatca gttactactt tcatattaaa
    47881 gattttatca ttttcatctt taaatgtgaa tttatcaact atttcacctt ctaaaaatct
    47941 tgtatcacct ggatctacga tttccacttt ttgcatcatt tgttttacta taacttcgaa
    48001 gtgtttatca tttattttaa caccctgtaa gcgataaacc tcttgtattt catttactaa
    48061 gtattcttgt acctttgtag gacctttaat agctagtata tcaagtggag agatgggacc
    48121 atcacatagt ggggtgccag ctttaacgaa gtcgccttct tgagcaagaa tttgtttagt
    48181 tatagggatt agataatgac gttcttcacc tgttttagaa gttataatta attctttatt
    48241 acctctttta agtttaggac cgaaactaac aataccatct atttctgcaa ctacagcagg
    48301 atccatagga gaacgagctt cgaataactc ttgtacacga ggtaatccac cagtaatatc
    48361 actggcttta cctactacac gtggtatttt agctataatg tcaccagctt ttattaattg
    48421 tccattatca accaaaacgt ttgcacctac aggtaaatca tatgttttga taacatcacc
    48481 attaggtgaa gttattaaaa cttgtggtat cttagtcctt tgcttagatt ctataattat
    48541 tctatcttta tggcctgtat tctcatctat ttcgtattta aaagttatat tttctattat
    48601 atcattgtat gcaactttac cagcaacatc actaataatt aatgaattat aagcatccca
    48661 ttcacaaata atttcacctt ttttcactac ttcaccttcc agtttgtata atttagcacc
    48721 ataaggtata ttagcagttt gtaaaatagt catagtatta ggatctaaaa gatgtatttc
    48781 ggcagaacgt cctataacga tataatactt gtttttacta ttgttttcat attctatgta
    48841 tcttaattca tcgaaatgaa caacaccatc agccttagca actattttac tttcgatacc
    48901 tatgccacct acagcacctc caatatggaa ggtacggagc gttaattgag tacctggttc
    48961 accaatagat tgtgcagcta tgacaccaac ggcctcaccg atgttaacta atttgcccgt
    49021 agctaaattt ctaccatagc atttagcaca aacaccatgt cgcgattcac atgtaagtac
    49081 agaacgtatt tccacttcag taataccggc atctacaatg gcttgagctt tttcttcagt
    49141 aatctcttct ccttcatgta ctattatctc gcctgtaata ggtttttgta tatcatgtaa
    49201 agatactcta cctaaaatac gatcatacat agtctctaca atctcatctc cattacgtag
    49261 atctcctact gttagacctc tcagtgtacc acaatcatct tcacttacta tcacgtcttg
    49321 agctacgtcc accaatctac gagtaagata tccagcatca gcagttttaa gagcagtatc
    49381 ggccaaacct tttcgtgcac cgtgtgatga aataaaatat tcaagcatcg aaagtccttc
    49441 tttgaaatta gctatgatgg gattttcaat aatttctgag ctagatgttg tacttttttg
    49501 aggttttgcc atcagaccac gcatgccaca caattgtctt atttgttctt ttgatcccct
    49561 tgcaccagaa tcaagcatca taaaaactgg gttaaagcct tgcttatcac tggcaatctg
    49621 ttttattagt atttgagtca atttagcatt agcatgagtc caagtatcta ttacttgatt
    49681 ataacgttca ttattagtaa ttaaacccat attatagttt aacgttatct cctctacttc
    49741 ttgcttagct tcgtttatta tatctatttt ttcttctggt actattagat caccaagatt
    49801 aaaggataaa ccacctttaa aagacatttt gaagccaaga ttttttaaat catctaaaaa
    49861 ttgcgtagtt actacattat tagtagcttt caaaacatca gctataatat ctctaattat
    49921 gcttttagtt atcaatttat taataaaacc tatttcttta ggaactattt gattgagtat
    49981 tactctacca atagtagttt ctattaaagt cggattgccg tctttatcat taatcattac
    50041 tttaacaata gcatgaatat ctgctttacc atcattatat gctatcaatg cctcatcagt
    50101 ggaataaaat attttacctt ctccccttac aggaaaatca tttgtgcttt ttcgagcttt
    50161 agttagataa tatagtccaa gtaccatatc ttgtgatggt actgcaatag gagcaccatt
    50221 agcaggattt aagatattat gagtagaaag catcaataat tgagcttcag caatagcttc
    50281 attacctaaa ggaacatgca cagccatctg atcaccatca aaatctgcat taaatgcagt
    50341 acaaacaagc gggtgcaatt gaattgcttt agcttcaatc attttaggtt gaaatgcctg
    50401 aatgcttaat ctatgtagag taggcgctct attaagcagt actggatgac ctctaagtac
    50461 attttctagt atttcccaga taacgggatc ttttctttca ataacttttt tagcagattt
    50521 aactgtctta acaatgcctc tttctaacaa atggcgaatg ataaaaggtt taaataattc
    50581 agcagccatt tcttttggca aacctacctc attcattcta agttcaggcc ccactacgat
    50641 aacagagcga ccagaatagt ctactctttt acctaataaa ttttgtctga aacgtccttg
    50701 tttgcctttc aagctatcac taagtgattt taatggtcta ttaagtgaag agcctgtaac
    50761 agcaacttta cgagtattgt caaaaagaga atctacagct tcttgcaaca tacgtttttc
    50821 atttctcaat ataacatcag gagccttgat atctaccaat cgtttcaatc tattgttacg
    50881 tattattact cttctataca actcatttaa atcactagag gcaaatctgc caccatctag
    50941 aggtactaat ggtctgagat ctggaggtat tactggtaat actttcaaaa tcatccattc
    51001 agggcgattt ttgatgcgag tttgtgcctc acgaaaatcc tccactactt gaaggcgttt
    51061 caataaatca gattttcttt gttgagaagt ttctttacta gctttatcac gcaattgata
    51121 agataattca tctagatcaa ttttagaaag caaatctaac aaagcttcac cacccatttt
    51181 agctataaat ttatcaggat tatcatctga gagttgttga ttttctattg gaatattttc
    51241 taaaatatta aaatattctt cttcagttaa aaaatcaagt tgattgatat tttccttagc
    51301 cattatacct ggattggtaa ctacatattt ttcataataa acgatattat ttacttgctt
    51361 tgaattgagt cctagaagtg ctcctatctt gttaggatta gagcgaaaat accaggtatg
    51421 agctattggg actgataatt gtatatgtcc catacgctcg cgtctaactt ttttttcagt
    51481 tacttctaca ccacaacgat cacagacgat acctttgtag cgtatgcgtt tgtatttacc
    51541 gcaagcacat tcataatctt ttactggacc aaaaattcgt tcacaaaaca aaccgtctct
    51601 ttcaggtttg tatgtacgat aattaatggt ttctggctta gtcacttccc catgagacat
    51661 agccagaata tcatcaggag atgctatact gattattata cttgaaaaat ttgtggggat
    51721 cgaatcactt ttaaatgcca tattttttac ctattcttca aatgttactt ttaaacctaa
    51781 accacgcaat tcatgaacta atacattaaa tgactcagga atacctggtt ctggtaaatt
    51841 tcgacctttt acaattgctt cataagcttt tgctctaccg gtcacatcat cacttttaac
    51901 tgttaatact tcacgtaata tatgagctgc accaaaagcc tctaatgacc atacttccat
    51961 ttcaccgaat cgctgaccac caaaatgcga tttaccacct aatggttgct gggtaataag
    52021 tgaatacgga ccaatagacc tagcatgcat cttgtcatcc actaagtgat gtaatttcat
    52081 catataaata acaccaactg tagttggctg atcaaaacgt tcaccagtac cgccgtcata
    52141 taaataaact tgcccgttat cgggtaaatc agctttttta atgtactcat taatttcatc
    52201 tattgtagct ccatcaaaaa ttggagtggc aaaattgaga cctaactttt tgccagccca
    52261 tcccaataca gtctcataaa tttgtcctaa attcatccta ctaggtactc cgagaggatt
    52321 taaaactata tctattggag tcccatctgg tagaaaaggc atgtctgctt ctgaaactat
    52381 tttagctact atacctttat taccatgtct accagccatc ttatctccta cctttaattt
    52441 gcgtttggta gctatatata ctttagccag ttttactatt cctgttggta ggtcatcacc
    52501 tacacttaga gtatatttat tgcgttgtaa tttaccataa aaatctttat atttaatctc
    52561 ataattgtga agtacttgtt tgattagttt atttttttct tcatcgagag tccagttttc
    52621 tggattaata tctagataat tttcaatcct ttgcaagttt cttgcagtaa atttttcgcc
    52681 tttaggtatt aattctacct tatatttatt atatacccca ttagaaacta ggccctcagt
    52741 aataaataaa agttttttaa tcaatttatc tcttaaatta ctagctactt tttcaaattc
    52801 tgcttcggct ttttctataa gatgtttttc cttttctttt tcttttttat ttttagtcaa
    52861 gcgagaaaat aatttagtat ctattacagt tcctgaagtg ccgggaggtg ttttgagaga
    52921 agcatctttt acatcaccag ctttatcacc aaaaatagct cgtagcaatc gctcttcggg
    52981 agtaggatca gtctctcctt ttggagtaat ttttccaatt aagatatcac cttctctcac
    53041 ttctgcacct actctaataa tgccattact atcaagatcc ttagtggctt ctatactcac
    53101 attaggtata tcattagtta actcttctac acctctttta gtttcacgta cttctgtcga
    53161 aaattcttcg atgtgtattg atgtatataa atcttctttt attactcgtt cagaaataac
    53221 aatagcatct tcgtaattat accctttcca aggcatgaaa gctgctaaaa gatttctgcc
    53281 aagggctaat tcaccttctt tagtagcaaa tccttcacat agaacttgac cttttttgac
    53341 tttatcacct tttttgacaa taggtattaa attaatgata gtattttgat ttgttcttag
    53401 aaatttagta agttcatata ctgtagcatc atcatcaaaa gaagctaatt tatcattctc
    53461 acccagatcg tatctaataa caattttttt agcatctaca tattctacaa tgccgtcatg
    53521 ttcggcatta ataagtactc gggagctctc agctacttta gcttctagac cagtagctat
    53581 aataggaact tcaggattta gtaatggaac agcttggcgc atcatattag atcccattag
    53641 agctctatta gcatcatcat tttctaaaaa aggaattaaa gaagcagaaa ccgaagcaat
    53701 ttgattagta gcaacatcca ttaaattaat ttgttcgcga ggtacaacag gaaaatctgc
    53761 accataacga cacattaata gatcttctac tattagtcca ttttcatcta ggagggtatt
    53821 ggcaggagct atgtaataat tttcttcatc ttctgctgtt aagtatattg gagattcatt
    53881 aatgtgaact actccatcta ctactctacg ataaggtgtt tcaataaatc ccaaatcatt
    53941 tatacgtgaa tagactgtta gagagtttat caagccaata ttaggacctt cgggagtttc
    54001 gatggggcat aaacgaccat aatgagtata atgaacgtct cggacttcga atccagcacg
    54061 ttcacgagac aaaccgccag gaccaagtgc acttactcga cgtttatgtg taagctccga
    54121 aagcggatta gtttgatcta gcaattgaga taattgattg gtaccaaaaa aagtatttat
    54181 aacagatgac aatgctttag catttattag atcaataggt ttaaatactt cattatccct
    54241 tacattcata cgctcacgaa tagtgcgagc catacgagcc agacctacac cgaattgcat
    54301 atataattgt tcacctacag ttcttactcg tctattgctt aggtggtcta tatcatctat
    54361 atcagtttta ttgttaataa gatttataag atatttgata atatcgataa tatcttcatg
    54421 tgttaataca cgctgattaa tatctacagt atctctacct aattttttat taatcttata
    54481 tctacctacc tctcctaaat catatttttt atctgagaaa aataatttat caataatttg
    54541 taaagcagtt tctttatctg gtggttcggt attacgaagc tgacgataaa tgtattctaa
    54601 agcttctctt tgattattag caggatcttt ttgtaaagta ttaaaaagta ctgaataatc
    54661 ttcagttaca atatcatctc tatgtaagag aatagtttct gtatcagatt ttaaaataat
    54721 aggtatatga atctcttcga taacagtttc tctttctatt agtacttctg tacgttctaa
    54781 agaagttaat tcacctgtat cttcatctac gaaatcttca atccaagtac gaaccacacg
    54841 tgctgcgagt tttctaccaa tgcaattttt aagattttct tcatttactt ctacttcttc
    54901 tgtaagatta aaaatttcta aaatatcttt atcagtctca taaccaatag ctctcaatag
    54961 agtggtcatg gggacttttt tctttcggtc tatatatgca tacatcacac tttggatatc
    55021 tgtagtaaat tcaatccatg tacctttaag tgggataatg cgagccgaat aaatcaatct
    55081 tccattaggg tgacgtgtag tttgaaaaaa tacaccagga ctgcgatgca tctgtgatac
    55141 tacaactctc tcggaaccat taataataaa agtacctttg ggagtcatat aaggtatggg
    55201 gcccaagtat acatcttgag taacagcttc gaaatcttca tgctcaggat ctgtacaata
    55261 caaataaagt cgagctttaa aaggtacact ataagtcaac cctctttcca tacattcttc
    55321 tatagtataa cgaggtggat ctacataata atctacaaaa tccaaaacat ataaacctct
    55381 agaatctgta attggaaaat tttctttaaa aactttaaat aatccctcat tttttctatt
    55441 ttctggagtt gtctccaatt gaaaaaaatc cttaaatgat tgcaactgta tctctaaaag
    55501 atcaggtgtt tcgtattgta aggatttgcc aaatttgatt ctttctttca tatttttaga
    55561 atctctttat tttattttta aaatttagaa atttattttt tagaatttat tataaaaaaa
    55621 tttaattata tatttattta ctttaaaaaa aatgcaaaag accagcctga acaattatat
    55681 aattgttcag actgataatt ttattattgc aaatatacat tttacttaac ttcaacttca
    55741 gctccagctt cttctaaaga tttttttagt gcttcggctt catcttttga aattttttct
    55801 ttaatagggt taggagcatt atcaactaat tcttttgctt cttttaaacc tagtcctgtc
    55861 atttctttta ctaatttaac aacagctaat ttttgaggac ctggagtttt tagaatgaca
    55921 tcaaattcag tttgttcttc ttgcgctgtg gcttgtccag tatcacccat gttagctcct
    55981 gcaaccataa cgggagcaga agctggttcg atattatatt tttcttttaa aattgtaaga
    56041 agttcattta cttctttcac agtaagattt actaactctt ctgctaatac ttcaatatct
    56101 gccatttttt gatattttta aatgttaaac aatttttatt ctttttctgc caatgttttt
    56161 aaaacaccag caattttttg tccttgacct tgcaaggttg ataataattt attaaacgaa
    56221 ctattaagag ctaatgcaac atcgcctatt agttcttcgc ggctctttag agtagctagt
    56281 gtattaagtt gatggtcgcc aataaatgct accccttcta tgaatgcacc ttttagaata
    56341 ggtttatctg aattttgtct aaatttttta ataagtttag caggtgcatt atttacaatt
    56401 gctccataca taatagctgt aggtccattt aagatatgat aaaactcaga aaaatcttta
    56461 tcactatttt gcatagcgat gcgaagcaaa gtatttttta caactttcaa tttgatattt
    56521 tctctaaaac aaagtgaacg taacttaaaa gtatctacag agtcaaggcc actaatatca
    56581 acaatataaa gaaaatcatt tttattgatt atctcagtta attcatctaa taaaattttc
    56641 ttatcttctt ttttcataat ttttaaaagt taattaatag atttaggatc aatttttacg
    56701 cttggactca tagtactgga agcagtgatt tttttaatgt aagtaccttt agttgtaggg
    56761 ggttttagtc taataatagt agtcaatagt tctttagcgt tatctacaag tttatcagtt
    56821 tcaaaagaaa ctttaccaat aggagcgtgg ataataccat atttatctac cttgaaatct
    56881 aatttaccac ttttagctgc tttgacagct tttcctacat ccatagtaac agtacctgtt
    56941 ttaggattag gcatgagtcc acgtggacct aagattttac ccagtggtgc taatttaggc
    57001 atcaaatcag gggtgcaaat aatgacatct atgtctgtcc aacctgattt aattttttca
    57061 atgtattcat ctaatcctac atagtcagca cctgcatctt tagcttcgtt ttctttatct
    57121 ggtccacaaa gcactaatac acgaacttct ttaccagtac cgtgtggaag catcacggac
    57181 cccctaacca tctggttaga tttgcgagga tctaccccca aaactacatc tagatcgaca
    57241 gaggcatcaa aattcgtgta agtaatatcc tttatgatct tcatagcatc agctaggcta
    57301 tagagtgcgt ttttatcata tttggttagc gctgtttttc ttttttttga tatcttagtc
    57361 atggttcatt aatttttttt taattttttt ttaatttaga atggaggatt accgataata
    57421 gaaattccca tgctattagc tgtgccagcg acaattttca tagcagattc tattgtaaag
    57481 caattcaaat cattcatttt atcttgagcg atttgcctta cttgttccca agtaaccttg
    57541 cctactttaa ttctattagg ttcggatgag cctttttgaa ttttagcagc ttccattaat
    57601 tgaaccacaa cgggtggagt tttcactaca aaatcaaaag atttattaga ataaacagta
    57661 ataattacag gtaatagttt accagcctga ttttgagtac gagcattaaa ttgtttacaa
    57721 aactccataa tattaagtcc cttagctcca agggcaggtc caatcggtgg agctggatta
    57781 gctgcacctc ccttaatttg taattttata attcctacaa cctcttttgc cattattata
    57841 ttattttttt taagattcct tttccacttg cataaaactt aattctaata gggttttacg
    57901 accaaatatt ttaacaccta tttttaactt tttcttttct tcatttattt cttctacagt
    57961 tcctataaaa gaactaaagg gtccatcaat aatacgtaca ttttcaccta ctaaaaaaga
    58021 aacttcaatt tcttcgcctt tatcatttaa ttcatctact ttactgagta tcctacgcac
    58081 ctctgcagga cgtaatggtg ttggtttatc tttatcgcct aaaaaattta acacaccagg
    58141 aatactttta atagtattaa taacttctcc atctaaaata gcttctacta aaacatagcc
    58201 aggaaaataa tttttttctt ttgtaatacg ttttccttta cgtatctgat aaactttttc
    58261 tataggtatc aaaactctag aaatataatt atgtaaattt aatttatcaa tttcaatttc
    58321 tagaagttct tttacctttt tttctttgcc agttatagtt cttataacat accatttacg
    58381 tagatcatta tctatatctt gcattattaa ttaataattt aaaatttttt ttaataaatc
    58441 aaacccatta taccttgact agctaaatcc ataaccaaaa ctacaacagc aatgattaat
    58501 gaagcaatag ccactaaaat agatccttct attaattcat cccaagaagg ccaagtgact
    58561 ttatacaaaa actcatctct cacttctttt aactgtctaa ttaatttttt cataaattaa
    58621 aaaatttgcg cgggtgggaa gattcgaact cccaaccaat ggttttggag accactactc
    58681 taccacttga gctacacccg cataatagta taacatattt ttttattaat ccaaaatttc
    58741 agttacttga ccagctccta cagttctacc accttcacga atagcaaacc ttagaccttt
    58801 attcatcgca ataggataaa tgagatctac ttggatagat atattatcgc caggtagtac
    58861 catttctaca ccgtctggca gagtgatttc accagtaaca tctgtagtac ggaaataaaa
    58921 ttgtggacga taatggtttt taaatggagt atgtctacca ccttcttctt tttttagaat
    58981 ataaacctca gctttgaatt tcttatgagg gtgaatacta ccaggcttag caacaaccat
    59041 gccacgtctg atctcatctt tatctatacc tcttagaagc aggcccacat tatcaccagc
    59101 ttctcctctg tctagtattt tacggaacat ttccactcca gttacgacac tttttaattt
    59161 ttcttcacta agtccaacta tttcaacagg atcacctgtg ttgataacgc cttgttctat
    59221 acgtccagta gctacggtac cacgtccagt aatagaaaat atatcctcga taggcattaa
    59281 gaaatcttta tcaatatctc taagtggctg agggatgtaa ttatcaactg catccatcaa
    59341 ttcgagaact ttctctaccc attgaggttc gcgattaaga gcaccaagag ctgatccacg
    59401 gataacggga acattgtctc catcgtatcc atagaagttt agcaaatcac gcacatccat
    59461 ttctactaag tctaataatt caggatcatc aaccaaatct actttattca taaaaaccac
    59521 aatagcaggt acgcctactt gatgagctaa taagatatgt tctcttgttt gaggcatagg
    59581 accatcagta gctgccacta ctaagatagc accgtccatt tgagctgcac ctgtaaccat
    59641 atttttgata tagtcagcat ggccaggaca atctatatgt gcataatgcc tattatcagt
    59701 ttcatattct atatgagagg tgttaatagt tataccacgc tctttttctt cgggtgcatt
    59761 atcgatatca taaaagcttt tagcttgagc catacctttt tcagataata tttgagtaat
    59821 agcagttgtt aatgttgttt taccgtgatc aatatgccct attgtaccaa tattgacatg
    59881 aggttttgta cgttcaaatt tttcttttgc catgtttttt gtattttttt tggtttaatt
    59941 agagccttcg gcgagagttg aactcgcgac cccttcctta ccaaggaagt gctctaccac
    60001 tgagctacaa aggcataaat atctaaaagc ggaagacggg gctcgaaccc gctacctaaa
    60061 gcttggaagg ctttcgctct gccagatgag ctacttccgc tagaagtggg gagggaagga
    60121 ttcgaacctc cgaaggctta gccaacagat ttacagtctg ccccatttga ccgctctggt
    60181 acctccccat ttactgagcc gatagaggga ctcgaacccc cgaccagctg attacaaatc
    60241 agctgctcta ccagctgagc tacatcggct ttttttttaa ttaaaatttt ttttaaatta
    60301 actctctaaa aatttagttt gcaaaattaa taatattttt taaaataaaa aaataatatt
    60361 tttttatttt tttttattaa tcttatttcc aattgtcttt tgttttttct atttgtctcc
    60421 ttagagcatc tacagctaag tcggtagctt cttcaaatgt ttttgctttc ttaattgcaa
    60481 aaagatcatt acccgcaatt attaagcgta tttcgacaat tttattttct ggtgctgaag
    60541 tgttatctaa ttttagtatt acttcgctac taattatttc ttgatgaaat ttgcttagtt
    60601 tatttatttt gtcagttatg tattcatcta actttttatc agttttgaaa tggattggat
    60661 taatatttac tttcataaaa aacctcctta tttattttat tatttttaag atctaggatg
    60721 tgactttgaa tatacttgtt ttatacgctc tatagaacta tgagcatata tctgagtaga
    60781 agctagactg ctgtgaccta atagttcttt tatagcattt aagtctgctc cctcatgtaa
    60841 aagatgtgtg gcaaaagtgt gtctcaaaat atgaggactt ttttgagaaa tataattaat
    60901 gctactcaag tagttattta ctgtacgata aacaaattta tcatataatt ttttgccact
    60961 atttaatagg aaaaatttat catctacttc tttattatta aaaaaaatat ttttgatatc
    61021 taaatattca attatgattt tttttaaatt attagtaaac ggtacataac gttctttatt
    61081 tcttttaccc aaaattttaa tagtttttct attgagatct atatcttcat tttttaaatt
    61141 tatcaattca gatctgcgta tgccagtttg atatagcatt tctatgatca atatatttct
    61201 tatagcttcg tagtcatttt ttatttcact tttgttagta ttttcaaaat cataaggtga
    61261 taagtctttt aacttaatgt attgaggtat acgcataggt attttgggtt ttgagatatt
    61321 ttttatagga tttttagata ttatttcttt attaagtaaa taattataaa atgaagaaag
    61381 tgaaacaatt tttctattta tggtcgaagc ttttagtcct gactgagata aattcattat
    61441 ccaatcattc acatgtatac gattcacttc taaaatgtca atatcaaatg tgtttttaat
    61501 aaattcatca aattgatata aatctatacg atatgaagat atagtattga atgaatatct
    61561 ttgattattt tctaaataat ttaggtattc ttttatccaa tccattaaat aagttaagaa
    61621 tattaataaa aactaagttt ttttttaaaa aaaatactta tgcattcata ctttttgtga
    61681 ttctacgttg catatataga gctcttgatt ttttctctct ttctctttca gaaggtttga
    61741 cataaaattg tctggctttt acttctcgag ccacaccggt tttttcatac tttcttttta
    61801 gttttttcaa agctttatca atgctatcac cttcttttaa ttctactatc aacatatatt
    61861 acctctttat tttttacaaa attaaataat ttttacatat tgacaaaatt tttctattcc
    61921 aaaaataatt tataattttg aatttgtgta aaaaaatata aaaacttatg aagcgtttat
    61981 tctcgttatt tttcttgatg ttatttattt gtttttgatg atttcttttt gcaaccaggt
    62041 gatgccattt atataataaa tgaagatgga caatttattg gtccaattac agatgtagaa
    62101 aacaaagaat ttaagatgta ttcgactgtg ccattaaatg gagaagaaat aactttatta
    62161 tatgtaaaa
//
LOCUS       DAOPRN010000004        61209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_365_length_61209_cov_104.952055, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000004 DAOPRN010000000
VERSION     DAOPRN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 61209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61209
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_365_length_61209_cov_104.952055"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..963
                     /locus_tag="PKI65_00910"
     CDS             73..963
                     /locus_tag="PKI65_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOB77030.1"
                     /translation="MNSDIRTRDAIRVSWIGFFVNLILCFFKLFAGIFGKSTAMLADA
                     IHSISDFVTDLIVIVFVSISAKQRDATHDYGHGKFETFASLLVSAILLAVGIGILING
                     INKVSYVIKGKSIAAPGMIAFIAAIVSIITKESLYWYTIKIGKKTNNQAVIANAWHHR
                     SDALSSVGTALGIGGAIFLGEKAHILDPIAGILVSGFIIWAALKILLPAINELLESSL
                     PENLENEIVDIIKSHPDVKDCHNLKTRKIGNIYAIDVHVKLDKNITFVHSHDVASEIE
                     SKIRNKFGKETIINIHTEPI"
     gene            complement(1127..1291)
                     /locus_tag="PKI65_00915"
     CDS             complement(1127..1291)
                     /locus_tag="PKI65_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOB77031.1"
                     /translation="MKKYECDICGYIYDPQKGDPDSGIAPGTPFEELPDDWVCPICGA
                     DKSEFSELED"
     gene            complement(1338..2090)
                     /locus_tag="PKI65_00920"
     CDS             complement(1338..2090)
                     /locus_tag="PKI65_00920"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOB77032.1"
                     /translation="MKKLENKIAIITGAAKGMGREEAILFANEGAKIVVTDILIDEAK
                     IVANEIIKNNGEAIAFKLDVAKAKDWEKLIDQIMDRWGKVDILVNNAGILNLCGIEEI
                     TEKEWDKIMEINAKGTFLGIKYVLPAMKLTKNGSIINISSIYGLVGSGAAVAYHASKG
                     AVRLLTKTVAAELAKYNIRVNSIHPGVIKTPMTDDLLKNPENAKNLLGTTVLGRAAEP
                     IEVAYGALFLASDDASYVTGSELVIDGGYTAL"
     gene            complement(2375..3583)
                     /locus_tag="PKI65_00925"
     CDS             complement(2375..3583)
                     /locus_tag="PKI65_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB77033.1"
                     /translation="MKTKSFLISMILLISVSSFAQSNKVLDAYNYLKAGKLDKAKAAI
                     DAAAQYPDTKDDPKTWLYKGNVYLAIAFTKDDKYKQLTPNPLDTALEAYKKCLILDPD
                     FTVSTAVPPHPNMGLKLIADEYFNIGATYFNNKDYNNALVYFEKSRNTRKIFTKEVDT
                     LANFYGAIAALNLQDTITAEKLLKELTTATNYNEPRVFTLLFDIYLAKKDTDNMLKTI
                     QLGKKRFPNDNDILLREINYYLITGDLEKSQLLLQEAITKDPNNPMLYYVIGTNYDQL
                     AQDTTLSQERRDLFFEQAKKSYQKAIELNPDFYEAYYNYGALLFNKGVEIFNKANKLP
                     PTEQAEYENLKKVYESLWHQSLPLLEKALTIQPDDIPTLYTLRTIYARLSMPDKLKEI
                     NQKIELLEKN"
     gene            complement(3640..6159)
                     /gene="gyrA"
                     /locus_tag="PKI65_00930"
     CDS             complement(3640..6159)
                     /gene="gyrA"
                     /locus_tag="PKI65_00930"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOB77034.1"
                     /translation="MEDNELTQSGGQIIDINIEDQMKTAYINYSMSVIVSRALPDVRD
                     GLKPVHRRVLYGMYELGLFSNRPTKKSARIVGEVLGKYHPHGDSSVYDAMVRLAQPWS
                     MRYPLVEGQGNFGSIDGDSPAAMRYTEAKLTKLAEEMLADLEKDTVDFQNNFDDSLKE
                     PTVLPAKIPNLLLNGASGIAVGMATNMPPHNLSEVVDGIIAYIDNRDITVEELMNYIK
                     GPDFPTGGLIYGIDGVIEAYNTGRGRVCIRGEAEIIEHNNKHQIIISSIPYQVNKSDL
                     VQKMAALVESKKIDGITDIRDESDREGIRIVIDMRRDANANVILNSLYRFSPLQSTFS
                     INNIALVNGKPETLNLIQLIHYYVEHRHEVIIRRSQYELNEAEKRAHILEGLLKALDF
                     IDEVIAIIRSSETVEIAKNGLMQRFDFTDIQATAIIDMRLRTLTGLERDKLQSEYDEL
                     MKKIAYLKHVLSDENLQMQIIKDELLEIKQKYGDKPRTDIDIHGKNVRIEDLIADEPV
                     VITISHLGYIKRTKLSDYRSQNRGGKGKIASDIRDEDFIENLYIATNHDYLLLFTRKG
                     KVYWLRVFEIPEAARNAKGKPIQNLIALDSDDKIQTIINTKNLDDHDAIKNDYVIFIT
                     TKGIIKKTSLTAFARPRSTGIIALNIRDGDDLLQVALTHGKSEIVVATSQGKTVRFNE
                     EEVRPMGRTAIGVKAITLSSKDDSVIGMVVMEDENQQILVISEKGYGKRSFLSAYRKT
                     HRATKGIKAMNITEKTGKLISIIDVTNENDDIMLITKNGIGIRLNISNIRLQNRVTQG
                     SRLIQLQNDDAIASVAKIAHDIDDDDTENIEAEISLEENNE"
     gene            complement(6221..7003)
                     /locus_tag="PKI65_00935"
     CDS             complement(6221..7003)
                     /locus_tag="PKI65_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOB77035.1"
                     /translation="MGKAINYIDTHAHIYLEDFDKDRKDVLERAEKAGIDLIILPAID
                     MSTFDAMWQCYQVNVNLQKMMIGLHPGSVKEDYKLELNFIENQLQNNVNSFIGIGEIG
                     MDLYWAKTYIVEQRIVLIQQLQWAIQYDLPVCIHQRNSLHEIISILDEFSNKPRGIFH
                     CFTGTYEEAKIIIDRGFMLGIGGIITFKNNKLAQTISQIGLDYLVLETDAPYLTPVPY
                     RGKRNESSFIPHIAKKIAEILKIDIKKVAFKTTMNAKNLFSL"
     gene            complement(6988..9972)
                     /locus_tag="PKI65_00940"
     CDS             complement(6988..9972)
                     /locus_tag="PKI65_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOB77036.1"
                     /translation="MRTKVIPFFLSILLFANAINFLNGQISIVQEEFYYHESTGMTIE
                     DYEHYNLMNKANMAPRKKDNCVLTKLVYGWHPYWQNGLQTNYDWNLLSHFVYFAYAID
                     PATGNPSTTQAFSTAQSVTDALNAGVKVHLCATLFSNHSTFLNNITAQRNFMHQIVTL
                     VLNRGAHGVNIDFEGMNSSHNAAFANFMNELADTFHTRIPGSEVTIALNAVEWSDVFN
                     INAMKNKVDYFVIMGYDYYWGGSTTAGPTDPLYNFVTSYNYTLTKSITHYLNKGCPPN
                     KLILGLPYYGREYGTVSNTIPSDVNNPPNCVSRTYKFVRDNTTGHYSATNKLWDQASF
                     TPAYIFYDGTEWRQCFIDDKYSMGKRMDLINQRGIAGMGIWALGNDDGYSDFWDAIRD
                     HFTTCALTPCVDSLFDMGGPLRNYYDNEDYTYTISPSNAQSITLNFTQLDLEPNNDML
                     WLYDGSSTSSQLIGSFTGNSLPGTLIANSGAFTLKFHSNSSINKSGFKLYYQCDQSPI
                     DNIPPITLISPISTWQTQDFSVIFQDSDAESGIQKAYYQVIEYDGIDWGANPHRGFLG
                     DNFDSPSINSRWNIITGNWVQENGCLIQTDSSIHNTHISTYLDQELSNRYLYEFEAKV
                     LGNNLTNKRVGFYFFSSDSASENRGNGYFIWFRLETGKLEFYKVTNDVFSKVHETNCP
                     LNLNQWYNIKVIYDRIDGGIWVYVNNLLKGKWIDSQPYNNGSYISFRTGNCSAQFNQI
                     KVYRSRYENATIHIGVGPDNSKELRYQSINTLSPAGKIKSIVQDNADNLSNTAMCDVY
                     VDYTPPIPPSYVWDGIGQDYDTIFLLPISTQWDHAQDPNSGISHYEVCLGSQPGYNDI
                     VDWINVGTNNFYSFNPTSLIYDNFYYITVKTFNNAGMSIQTFSDGFRYIVVDQISIFN
                     QNNKPWIIYPNPANDHIYIKGDINIEKLSIYSLLGAKIDCPIIEKHNNKVAIQIDHLS
                     SGIYIFQINSGNQIYFYTWEKQ"
     gene            complement(10171..11394)
                     /locus_tag="PKI65_00945"
     CDS             complement(10171..11394)
                     /locus_tag="PKI65_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOB77037.1"
                     /translation="MKIDRYLASKLIQSKQEGSARPILIIAQISIAISIFAMLISFAI
                     IRGFKTEIKEKVVGFNGHLQIANYSSLVEVYTSDPIAYPRDDVENLKSISGIVSIAPV
                     IEKPAILHEDNLIEGIIFKGINADYPKDFFEKNLVKGRFPNLDTALTEVCISKNIAQK
                     YNLDIDSAVRIYFFKEGQYTPRGRKLKIVGIYNTGLQDYDNHYVLGNIKIPQQIDGWS
                     DSLVAAYEIYLTDIDKIDIIKPLILQRLNYDVQLIDVRDKENRFFSWLEMLNSNVVVI
                     YILMAIVAFINIISILLILILEKIKFIGLMKTLGMTTNNLSKVFLWITMRYICYSLII
                     GNVVTILLIIAQNLWHLIPLNEAMYYMSYVPMQFDFDYFIFANLAIIFLGLLGIVIPR
                     LVIKQISPAEALKIE"
     gene            11507..12157
                     /locus_tag="PKI65_00950"
     CDS             11507..12157
                     /locus_tag="PKI65_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOB77038.1"
                     /translation="MIKLENIYKSFKPVEVLKGINLEIKENEFISIVGPSGAGKSTLL
                     HLIGTLEKPDSGTVKYNDIDIYKLNDKELSDFRNKNLGFVFQFHHLLPEFTAIENVML
                     PVLIGGLNKDEAMDKAKILLENLNLSHRFTHRPQELSGGEQQRVAIARALINNPKVLL
                     ADEPTGNLDSKNAQEVINLFKKLRDECKRTIILVTHQESFAKISDRIVYIKDGVIE"
     gene            complement(12197..14188)
                     /locus_tag="PKI65_00955"
     CDS             complement(12197..14188)
                     /locus_tag="PKI65_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOB77039.1"
                     /translation="MKKIYIFFASLISILSISAQDYNCTSIPKSKHQQKLDLAISKIY
                     QGMQSKFIESQKIYFSEATEILKSINENHEYPIVNYYLGLTYIYKPDNNFYLAEQYLK
                     KAIDACPDDIPEAYFHLGKLAYTNNDKCEAINYFKRFLSYEGDFDTLLDEARLLLKWS
                     IEICDIISNPVPFEPKLVKGISSPLDDYLVSLSPDNEIAYYTRKTKEYQLSGYKSEPA
                     FQERFYYSYRIGKNEFDNGKLMPYPFNRNENEGGATITGNNKELIYTVCIMVNSPRQY
                     YNCDLYSSRYVKGYWTDIIPLDRVNRPDTWESMPSITADGQELYFVSNRPGGIGGYDI
                     YKSIRDQNGEWSEPINLGRPINTPGDEKSPFIHTDTRTLYFSSNGHPGIGGYDIFYVK
                     LNDNDDKSYLKVKNIGFPINTENDEVGFVVSLDGRYGYFASNNIKNQSIGGMDFYYFD
                     LHPDAKPEEVLLVKGNLKSQDTTQSVKATIQIKSMESKKVSFIPVDDEGDYAATLLKN
                     EDYLLTIKGEDIVYQSTYISARDSLEASIIELEMEVQPIELGMHYRLHNIYFAFNSAD
                     ILPSSQKVLDEFIIFLNDHPTMKISIEGHTDYIGSDDFNLILSEKRARAVYDYLVING
                     INKNRLEYKGFGKQRPIATNDTEEGRAMNRRTEFVILSY"
     gene            complement(14337..16382)
                     /locus_tag="PKI65_00960"
     CDS             complement(14337..16382)
                     /locus_tag="PKI65_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HOB77040.1"
                     /translation="MKNFLKIFLCVILCILCIPRLNSKNTYDIDKEFIDIITKRSLDD
                     ITDNLKNIKDTIKIFNILDTLENQALYKNDLNLRAKIEFTRGCLHYLGSNHIQSLKHF
                     STSYRIFTSLHDTVGIVYSLYYQSVIAFNINLNSIIVSNFFEAKDYLPGFKNDSLLIE
                     FYRLLGSAYFNMGFYKESIDAVQSAIQLSYHINSKEQLLRSYMNLALNFIELKKYKEA
                     EEILLYVLEKAQESNNYNLIGTAYSYIGYLYNEFGEYEKGIEYNKKALEFRKKINDKR
                     GISTELNNIADGYYKLGNYTMSIEFSKEALKYADSANLTNAKISANFILAQSYACLGN
                     YKGAFDALLKSYTAYKEKYNQNFIQEVLDILVKTSYDYLINENMALKQKLEINAKLIK
                     NNRNLQFFMVLIIIITIIIAYFLFRNTMKNRRLVKEMAKINLELIETNKKLIENEEAL
                     KIANATKDKFLSIIAHDIRNPIASMISFIRIMRRDFNDLTKEERNALIDELEKVVNNT
                     NQLLDNLLLWVRSQSGKIEVKIVDTNLNNIISHVQDLFTSIAKNKNIDLIIEGDTNID
                     IKTDVDMLNTILRNLVNNAIKFTHPQGTVTISTEISNGNLIISVSDTGIGMSEELQEK
                     LFKIGEKVVQNGTNKETGSGLGLLLVKEFVTLLNGKLELESEKGKGTTFRIYLPLDF"
     gene            16663..17532
                     /locus_tag="PKI65_00965"
     CDS             16663..17532
                     /locus_tag="PKI65_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOB77041.1"
                     /translation="MLTSFFARLLQILIGYTPHFLLFFWDYFIYFITYHLLHYRKKVV
                     QKNLKLAFPDKSKNYYKKIEKQFYHYFARLIIDIAINGTTNKTKRLKYLKFENINEIK
                     KCLSEGKNVMIALGHLNAWTTQIILSEVLEVPVYMVYKPLSNKYIDNWLRNNLHSPNT
                     IFIDNRSAARHILKVSKQGTPSVFLMVADQSPATDTGVHWVDFFGINTPFHRGIEFLA
                     TKFNMPVFFADILYIKPFEYTTKYHLIYNGEEKVESKTITQRFANVLEKAICEHPGDY
                     LWSHKRWKKVIKY"
     gene            17981..20347
                     /gene="feoB"
                     /locus_tag="PKI65_00970"
     CDS             17981..20347
                     /gene="feoB"
                     /locus_tag="PKI65_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOB77042.1"
                     /translation="MTLNDLDIGQSGYIVKIKGNETFRHRLMEMGFKTNENVTLLHKA
                     PLNDPLLFQVMGSKVSLRRSEAINIEITTEEELQESENIDILSTNIQHLITKKSKNIE
                     VALIGNPNSGKTTIFNYVSHAHEHVGNYAGVTVDAKSGTIEFKDYKITFVDLPGTYSL
                     QVFSPEEAFVRHYLLKKMPDVIINVIDASNIERNMFLTTQLMELDYPMIIAINMYDEL
                     TKKGAKLNIDKLEEMFKIPIIPTIGIKNKGIDKLLSAVIDRYESTLTVKSVVHFPLPV
                     EACIEKIENEIAKTNFTKSAISQRFVAIKLLEKDPEFHRFLKRNEYASLNNVVDKCIE
                     NIEKQYNRPSEFLFTDVRYGFIKGALKETLSYSKEDADQSIDKLDKILTHKIWGIPIF
                     LAFLYVMFFSTFYLGSYLQNWMESGVDWFKVFLSGFLPASLFKNLLIDGVITGIGSVL
                     VFLPNIMILFLFIAFMEDTGYLSRVAFLMDKLMHRIGLHGKSFIPMIMGFGCNVPAIM
                     SARILENPSDRKITILINPFFSCNAKLTIYVFIINIFFPNSLAYMPFILYLTGVLIAA
                     IVAILFRKFLFAKESSPFVMELPPYRMPTFNAIIKHMWFRASMYLKKIGTTIFIASIV
                     IWFLSHFPLDNDNTRLIETQIARITETETNTALLDSLHTQLVYERISNSYLAKISMFL
                     EPIFKPLGYEWHVISALISGSVAKELVVSTLSIVLPNRGAMANILSPPSAAGLLFFAL
                     LYCPCIATLSTIKKETGSVWWAMFTVVYTTIIAYGVALLVKSIFTLIL"
     gene            20435..21475
                     /gene="thiL"
                     /locus_tag="PKI65_00975"
     CDS             20435..21475
                     /gene="thiL"
                     /locus_tag="PKI65_00975"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOB77043.1"
                     /translation="MSLTPLNNIGEFALLERLAKLINIYNKDKVIKGIGDDAAVIKPD
                     IDLVQVVTTDMLVENIHFDLSWMPLKHLGYKSIVVNVSDVVAMNAIPAHVTVTLAISN
                     RFTLEAIDELYEGIRLACENYKIDIIGGDMVSSNQGLIISITAMGYINKENLVYRNGA
                     KPNDLICVTGDLGTAYAGLLILQREKKTFMANPYYQPDLQNYEYVIEKQIKPEPRIDI
                     INKLRELNILPTAMIDISDGLSSELHHICRQSNTGCRIYEDHIPIDMMVSNIADEFYI
                     SPITMALHGGEDYELLFTIPLNQYDLIKNIDDISVIGHITDANEGMYLINNIGEAIEL
                     KAQGWDSFKKEE"
     gene            21543..22289
                     /locus_tag="PKI65_00980"
     CDS             21543..22289
                     /locus_tag="PKI65_00980"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOB77044.1"
                     /translation="MSGHSKWSTIKRKKEANDAKRSKMFTKIIKDITIAVREGGPDPD
                     SNPRLRLAISNARGVNMPKDNIQRAINKAIEKDSASYVELVYEGYAPHGVAIIVECAT
                     DNQQRTISNIRSYFNKHGGNLTTTGSLNFIFDQKGVFSIKRDQIKNFDDFMLEIIDAG
                     ADDIIEEDDFVIIYSARENFGNIQKALERLSIEAESSKLERIPNNYIKLEKEQALKIL
                     KLIDLLEDDDDVQEVYHNLELSDELIEALE"
     gene            complement(22294..22548)
                     /gene="rpmA"
                     /locus_tag="PKI65_00985"
     CDS             complement(22294..22548)
                     /gene="rpmA"
                     /locus_tag="PKI65_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOB77045.1"
                     /translation="MAHKKGVGSSRNGRESESKRLGVKKFGGQYVKAGNIIVRQRGTK
                     HYPGENVGIGRDHTLYALKDGIVEFKKTRGNKSYVSIKVN"
     gene            complement(22565..22876)
                     /gene="rplU"
                     /locus_tag="PKI65_00990"
     CDS             complement(22565..22876)
                     /gene="rplU"
                     /locus_tag="PKI65_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOB77046.1"
                     /translation="MFAIVELGGKQFKITEGKKLYIDRLNAELESILEVDKVYLIAND
                     EGVKVGKPIVEGAKVTIKVLEHLKGPKVTVFKKKRRKGYRVKKGFRPYLTKVEVLNIK
                     A"
     gene            complement(23085..23906)
                     /locus_tag="PKI65_00995"
     CDS             complement(23085..23906)
                     /locus_tag="PKI65_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOB77047.1"
                     /translation="MEIEIKPPGKWSFFDVKELAFYKDTLYFLAWKDIKIKYKQAVLG
                     FLWAFIQPIAMTAVFVIFNLAINVTLGDVNIPYPLFVLSGVMLWNLFGNAVTTTSNSV
                     VSNANILKKIYFPRIFFPMSSLLVNLFDFFITLLIYIPVMVYFKVYPNWDIIYILPIC
                     VLLVSILVLGIGSFFGALNVKYRDVKYVIPFIIQLLFFISPVFYSNNQIAIPWLKSAY
                     YLNPMTGVIELFRYGLFGTQINQFGLSISIIISFVCLIVGLYYFRKTEYFFADIS"
     gene            24012..25307
                     /locus_tag="PKI65_01000"
     CDS             24012..25307
                     /locus_tag="PKI65_01000"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOB77048.1"
                     /translation="MLNKKIIKSIFIFFLPLYLFAQYDYYIYDIENTFDGVYDERFAT
                     ILLLDTSWEFKFPVIDLAKGNALQLSFDMLVDEVYDLYVVFSPCSPEWQILDEPITDF
                     IEGFNENPIFDYSFSRNTLISYINYYYYFPNEDIKFKRSGNYLLTVYYHDKENNKYSK
                     AFSKRFVVSEQALLVNAHVIFASDVTERFYRQQVLFSFTSNNIVFNNIYERLYVCLLQ
                     NGRWIDAHCNIKPTYMKDNIVYFDESAKNFTTFDGNNEFRYFDIRSISYQTGNLAGIM
                     HDSETVHVYLVPDISRRYTQYLRQPDLNGRFFISNDLYSNINTESEYYYVHFAIKSEP
                     ITEGAVYLIGDFTNYEIIPRYRMQYNYEAKQYELCLLLKQGYYNYAYALVPNQTDILN
                     ISYFEGNHSETENEYQGLIYYREESDRYFRLLHVFSISK"
     gene            25385..26233
                     /locus_tag="PKI65_01005"
     CDS             25385..26233
                     /locus_tag="PKI65_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOB77049.1"
                     /translation="MNKVRTAFLLGLMFALFMGVGYLIGGESGMFIAFFIALLMNGIS
                     FFYSDKIALSMFRAKEVSESEAPQLHRIVEELATEAGIPKPRVYISNIEVPNAFATGR
                     SPKHSAVAVTKGLLEILDQNEIKGVLAHEIAHIANRDILISSLAAVIASAIAMLARVA
                     YFANIFGGDNKGGGIGNLLATLIMIIITPILAMIIQLAISRTREYVADEKGAKLARNP
                     LYLANALRKMDAYAKMKPVTENEINPAATHMLIINPFKKGGLAALLSTHPPIEKRIEK
                     LEAMAY"
     gene            26334..27308
                     /locus_tag="PKI65_01010"
     CDS             26334..27308
                     /locus_tag="PKI65_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOB77050.1"
                     /translation="MNNAIVVSNITKRYREHTALDNVSFEVPTGSIFGILGPNGAGKT
                     TLLRIINNIIPPDNGEILLFDEPLTDKHMEQIGYLPEERGLYKKMSVGDQVLYLSQLK
                     GLSQKQARERINYWFEKLEIKSWLGRKVEELSKGMQQKVQFIITVIHSPRLMIFDEPF
                     SGFDPINVDILKNEIIELKNKGATILLSTHNMESVEELCDEIVLIDRSKLILYGEVDE
                     IKRKFSKNHFEVTFAHPIDDKKIENWQKNHPHFTINRVNKNNNDKNTISITSNLPIKS
                     NEILLLVIELGEIISFREQLPSMHDIFVTVVQQKEEIYDHEEQITYNN"
     gene            27280..28614
                     /locus_tag="PKI65_01015"
     CDS             27280..28614
                     /locus_tag="PKI65_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOB77051.1"
                     /translation="MKSKLPIIIKREYLNIVKKTSFIVITLLGPIILASIFVIPIYIS
                     HINEAQISVGVIDETGFIYKGLETNALARYTLLYLPVDSAKKLLDEDKYDAILYIPIS
                     FINSPQTPRIWSKNAINQSVINNIESVLKSELEKHRLALYGINNQILEQINADVKVNN
                     YTYKGGVEKVASSTVATILGFVMALIIYFFVFMYGSMVLRSVMEEKLNRIVEIIISSV
                     KSIHLMIGKVIAVLLVGLTQFVTWILLTVLIIFGVQQAFPQLFKYTEPEIVNIQTKGL
                     TQSEAQQIANAAKLQESPSNNALLALANINIPLYLLLFLFYFIFGYLIYATLFAAIGA
                     AVDNQDDTNQFMLPITIPLILAIICLQPIFNNPTGGVSIFFSLFPFTSPVIMIGLIPF
                     NPPLWLIITSMAILVLTFILSAYISSKIYRIGILTYGKKPSYKELWNWLKKY"
     gene            28893..31286
                     /gene="topA"
                     /locus_tag="PKI65_01020"
     CDS             28893..31286
                     /gene="topA"
                     /locus_tag="PKI65_01020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOB77052.1"
                     /translation="MAKQSKEEKKEKKSSTSKKTTNKSSTYTLKGKNLVIVESPAKAK
                     TIEKYLGSDFTVLSSFGHIRDLPKGELGVDVEKNFEPKYVIIKGKEKVINQLSAASNQ
                     SELVWLATDEDREGEAISWHLAEALEIPEDKLKRITFHEITKSAIEEAIKNPRGIDYN
                     LVNAQQARRILDRLVGYELSPVLWRKIAKNLSAGRVQSVGLRLIVERDREIERFVAEE
                     SYKISGNFFLLEKPDILLSAELNHEFKKEKDALNFLEKSKNAIFTIKDIKQHPAKRTP
                     PPPFITSTLQQEAAKQLRFSVSQTMNIAQQLYEAGLITYMRTDSVTLSNLALNAAKEF
                     ITKNYGEKYYKRRQYKNKVKGAQEAHEAIRPTDLSKSTIEATQQMKRLYELIWRRTIA
                     SQMSDAEFIRATISIASDQNKHLFLAKSESLVFDGYLKIYQEKDDDDDVKEITSAIDI
                     SKFKIDDRLDFKDITAKQSFTNPPYHYNEAALVKKLEELGIGRPSTYATIITTIQQRD
                     YVDKRSIPAKERNIKIYTLDNSKKIKDTEKKEKYGGEKQKLISTELGKVVTDFLMKHF
                     PDIMDYNFTAEIEERFDKIALGQEEWQDMLRDFYKDFHPKVLEAIETSSKQHISRLLG
                     VDPKTNKNVYITYTKHGMALQLGDYTDNEKPSYVSINKRQNIDFNKITLEEALKFFEL
                     PKELGDYQGSKVVVNIGRYGPYVLYKDKFYSIKKLDKPLTEFTLDEAIEIIKQPTGLI
                     KTISEKPLIQLLIGRYGPYIKTDDGNIPIPKGTDINTITDDEIKFIIENHKTKKNKK"
     gene            31283..32443
                     /locus_tag="PKI65_01025"
     CDS             31283..32443
                     /locus_tag="PKI65_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOB77053.1"
                     /translation="MKELQYLFEPCFYDFSFNNHKSHLYSNVITIDKLDDKSMSDINS
                     LAIINFSINTGKKNFINSLYKLRSYLYSYRKIDNVQIIDLGTFINNTDAFENINGLAY
                     VLSDLLLKGALPIILSDDRSVVLAIHKAYELIKRFYNLAFILPFIDVNADNLWEQSKD
                     LLPWHNEIFKDNNLLNNATFIGYQSYLTDNEALEWVENNFYDTLRLGDIRENIKATEP
                     YIRNSDIVCFDLNAMKKAEAPATMLTLPNGLYAEELCQLAFYAGMSDKSSVFTFYNYY
                     PDKDLQDQTIQLLAQTIWYIVEGVSLRVKDYPFTAKTQMIKYTVIMDSAVKELIFYRS
                     PRSDRWWIELPIYNTSQIQRHTLIPCLYEDYLAALQNKIPHIFWNAIKKSKK"
     gene            complement(32591..33694)
                     /locus_tag="PKI65_01030"
     CDS             complement(32591..33694)
                     /locus_tag="PKI65_01030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOB77054.1"
                     /translation="MGKNIKLLIILLTALPSIVLSQQKIEIFGKFISPTQYSEITLSN
                     YLNPAALTIKVPIINSDSFYISLEQTSAEEIYKLYFDIRTNFLALVLKPGEKVHLLLN
                     PYQLGNKPIIKGSPSSIMVYEVEWILANLQKQLDSLQRLYIQTKSIDTKAIIEQEFIN
                     LHNVKNTYINNIVRSNPSSLANLFFIERLNIDDGYAETYKIVDSALLHNYPHHPGVLN
                     FHNKTQALLRTSIGSKAPNIKLPSPNGDTLELYSIKGKLILVDFWASWCGPCRKENPL
                     VVKAYQTFHPKGFEVFAISLDKDKQSWINAINTDKLTWHHVSDLKYWNSEAAKLYGVS
                     VVPYNFLLDENYNIIAKNLRGQQLYNFLNHYFK"
     gene            complement(33744..34496)
                     /gene="truA"
                     /locus_tag="PKI65_01035"
     CDS             complement(33744..34496)
                     /gene="truA"
                     /locus_tag="PKI65_01035"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOB77055.1"
                     /translation="MRYLLELSFVGTNYFGWQKQKNLPTIQGELDKALTIFFRQDLTT
                     IGCGRTDTGVHAINFFAHFDININIENPNRILYNLNAILPTEIAIKNINSVQDDFHAR
                     YSAISRTYHYLIIKNKNPFFINRAWQMSKPLNIDNIKKLLPSLIGIKDFSAFAKKMPI
                     SNYNCNIHEIIVKEHKDFIIFSITANRFLRQMVRSIVGVLTSIGLGDIDEKTFNDLLI
                     KKDKNKIKLIAPPQGLYLTHVCYPENLTHIVK"
     gene            complement(34549..35766)
                     /locus_tag="PKI65_01040"
     CDS             complement(34549..35766)
                     /locus_tag="PKI65_01040"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOB77056.1"
                     /translation="MKILVLNCGSSSIKYQLFDMLADETVLAKGLLERIGLPMGIVNH
                     TPIGKEKIIYEQPIPNHTAGIEIIIKILIDKELGVIKSLNEIAAAGHRVAHGGEHFKN
                     SVLINNETKSEISKCIQLAPLHNPANLKGILAVEQLIPGLPQVAVFDTSFHQSIPDYA
                     YIYGIPYEYYEKMEIRRYGFHGTSHKYVSDKSAKLIGKNIDDLKIISCHLGNGASITA
                     IENGKSIDNSMGFTPVEGLIMGTRCGDLDVGALLYIMEKENIDTKQANDLINKKSGVF
                     GISGLSPDMRDIESAAKNGNKRAQLALDVYAYRVKKYIGAYAAAMNGLDLLIFTGGIG
                     ENSSFMREKIASDLEFLGIKLDSNLNNSAKGKDALISTTDSNAYIVVATTNEELVIAR
                     DTMCIVNDNLSKN"
     gene            complement(35812..36666)
                     /locus_tag="PKI65_01045"
     CDS             complement(35812..36666)
                     /locus_tag="PKI65_01045"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOB77057.1"
                     /translation="MRFRGIHAILILALSLVLLLLGIAGIIYLHGTALENKLKENFTI
                     QIMLKPTASEVEISLLKHNLEATGKFSAINYIPSMKMLEELQAELGDISADKLELENI
                     PLPSLLEATISADYLNDEVIKNLVTQIKQKDIVDEVYYPTNVLTEIEKNKNTLSTITL
                     VVAIIILAIAVTLLSLTIRLTIYSQRFTIRTMLLVGAKKGFIQKPFLKDALFWGVLAS
                     ILAILGLEVIINQLYKLIPELNGVVTQQHFIFLFIFIFIIGVVITELSTLFSVNKYIK
                     MKTDSLFY"
     gene            36864..37826
                     /gene="trxB"
                     /locus_tag="PKI65_01050"
     CDS             36864..37826
                     /gene="trxB"
                     /locus_tag="PKI65_01050"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOB77058.1"
                     /translation="MSNITGEEKRNLVIIGSGPAGLTSGIYAARADLHPLLYEGLISG
                     GQLINATLIENYPGFPDGIQGMELMEKIKKQAIKFGADLRFSSIEKVDFLQYPFLLWD
                     DNDIAISALSVIIATGSSPRLLGLPSEKEYLGYGVSTCATCDGYFYKGKVVAVVGGGD
                     TAAIEAIYLANLASKVYIIHRRDQLRACTCNQKKIFNTPNIEVIWDSVVEEIKGETKG
                     FTKNVNAIVLKNVKTNELKTINVDGVFIAIGHHPNTELFEGQLELTNDKYIKTIPGST
                     KTSVEGIFACGDVRDPIYKQAVIAAGSGAMAAIDAQKFLENFNF"
     gene            37913..38386
                     /locus_tag="PKI65_01055"
     CDS             37913..38386
                     /locus_tag="PKI65_01055"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOB77059.1"
                     /translation="MKKEYLGLEYYIFDNLEELDKKDKELVKNAIDASTRAYAPYSKF
                     HVGAAVRLSNDIIIQGNNQENAAYPSGLCAERVTLFYAHSRYPNEVIVTIAIYANNED
                     NTYKDIVTPCGACRQVMAEIQSAQKKPLRVILLSPTNSGIILPSIDVLLPFQFEL"
     gene            38405..39586
                     /locus_tag="PKI65_01060"
     CDS             38405..39586
                     /locus_tag="PKI65_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOB77060.1"
                     /translation="MKKIKAYLILVIFFILSIIVCCKKNVPQGHVSISGRLIGGGGKP
                     LYFATVHPKEIELLDSCTLNEEGFFKIKLAIAQPQFIKAYSNDTNFIILAAHPKEDIY
                     ISGDYYQLDATYSVFGSPSSEIIHKYRSFTRKNLEKLHMLSIYWENHKYDNDNLNIKD
                     SLDRKALEIFKNQRDSALALINKNPEDFANIFIIYQFFINQPLFDEYHDLEIYQWVLS
                     NLEKNYSDNEHVINLKMRINKIKTKIAERDSILSRLAIGKPAPDFALPLWQSDSEFVL
                     SLERGKSLLLHFFAGASLSSVKDMSKLKKLLPKLSKYKIKLITIFLDYDMETIARAID
                     GEGINWTICYDLSSTRSPVAKLYAVEQLPMYYLIDANGIIRDKAFTADSINVSSINTI
                     Q"
     gene            39583..40365
                     /locus_tag="PKI65_01065"
     CDS             39583..40365
                     /locus_tag="PKI65_01065"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOB77061.1"
                     /translation="MKKQISYIPYSLAKEKKLYFISDIHLGMPNESDSKNREILLVQW
                     LEQVSSDAQAIFILGDLFDFWYEYKYVVPKGYTRLLGQLAKMNHSGIEIHFFVGNHDM
                     WTYGYLENEIGFKIYSDIAGFYIDNIRILLGHGDGLGPRDYGYKVMKKIFKNNVAQKL
                     FSMLHPYFAFKIAHRLSSTSRGDYEKHIEEFKGIEKEFIIQFLASLDESIKPDIAIFG
                     HRHLKTDLLINDIRYINLGTWLLEPNYAQFDGSNVLLKTFFK"
     gene            40605..44144
                     /locus_tag="PKI65_01070"
     CDS             40605..44144
                     /locus_tag="PKI65_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOB77062.1"
                     /translation="MIKIDYIINKKLKLSLWILLISVLVFTINHTFYPLYQSINKNNL
                     SKKVEKQWNELKSFVNDTAIIKLIINNDWSSLQQELENINNIKAGVWIYDADSLIYWS
                     TNDFIINNLTAQQWVAESERGIFLCYSQEFENYKLILSWQLQESYPIKNNYLTNKINK
                     KIDPLAAFELKINNKNNNVLENLEFIPRDIQPTKTQNFIALLSFILFLFGLYLLISAL
                     INKWKASNKIFVLLILILTIIGLLWILTNFLSTKYYVFNPSFFALSLIFNNIGKTFLL
                     VWLAALIIKIAIKLLPNDYKRLFCFVTSIFFSGNIFIWLSRIFNATEFPYSYYEWIST
                     KVDIIFVIILAISLLVIWTWSIRLMLTHKPPKKIVYSGFAFYTIFLIFLLKNQNFYLI
                     ISMLGILLWLIMYLLSSMNKKSFRRYFIYIFSGMIVAISSFLFMRVHHQSFSNYLLLN
                     AVKISSERDLILENHLNELQTEISADRFLVNYANSFDTSMQWLTHIKNHYLSSFQDQY
                     DIIGFICSSHDKFLNNNLEINCWQYYDSIVEYSQAQHISDNLYFITNANITYYLYKIM
                     LHSELPHQLYLELYKKKPFRPEGYLELLVNIEEEQLLPGQLDYATYYNNTLMQSYGNT
                     IFPLQVDNEIDAAPIGKLFKYHDKYAIKSEIDNNRFIVLVSNHTLPNWLSIFSFFIII
                     ILLLYLIYYILIKGINIKKIFRSYAGRLQITLISIITIVILVAGILSVKYIQNTYSRQ
                     LEKQCFERNYGLYLSLQDEIVKNNIKINSTSIDSLLWKYKYSYFSDLHFYNTDGTLLS
                     TTQFALFNDHLLTTLINPDVYWTNKWKKYWYYSNKEKISEFEYRNTYMPVFRGNNIIG
                     ILSIPMFSQITELRGELSELIGAFINIYAILLLISLIFTFLITQYINRPLQLISKRLK
                     SLNLSKQNEKIEYKSNDEIGQLVKQYNQMIDELAEKAEKLAQNEREIAWREMAKQVAH
                     EIKNPLTPMKLSVQFLQRIWKQDKKRFEQDIEKYLTTLLEQIEQLSEIATAFSQFNKM
                     PEPLSKEVNLITLLQNLAALFHSTDYDINIHLESDKLTVIGDESRLNRAFTNILQNAV
                     QALSNERRGEINIYAKSDNNNAIIIISDNGIGIANEMLENIFEPNFTTKSSGTGLGLA
                     IVKHIIEDLNGKILVESLLGEGTKFTIILPLCY"
     gene            44218..45558
                     /locus_tag="PKI65_01075"
     CDS             44218..45558
                     /locus_tag="PKI65_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOB77063.1"
                     /translation="MSEPNIKTKSLPKIFVLDTSVILYNHEAIYSFDDNFIAIPITVL
                     EELDNFKKGNELINFEAREFIRILDQISYNHSINEWIQFRENGASVRVILDEGSDPDA
                     NKIFGGHKPDHRILNVAIKLSKEFPDRKVILVSKDINLRLKARALNIMAEDYETGKIK
                     DLEKLYTGKTTLEIDDKSIIDLMYKQGFVEPNEIGITDPVPNHYFILKYNSNSVLGYY
                     NPLTQVIEKIVKKPAARIYPRNAEQVFSIHSIVSPNIKLVTLQGVAGTGKTLLALAGA
                     LETKDDFKQIYLARPIVPLSNKDIGFLPGDIKSKINPYMEPLYDNLRFIKSQNNNYAF
                     QQNIDKMLEEDNLVIAPLAYIRGRSISHVCFIVDEAQNLTPHEVKTIITRAGEGTKII
                     FTGDIRQIDTPYLDARSNGLSYLIDRLKGHPIYAHVTLEKGERSELANIANELL"
     gene            45555..46229
                     /gene="lipB"
                     /locus_tag="PKI65_01080"
     CDS             45555..46229
                     /gene="lipB"
                     /locus_tag="PKI65_01080"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOB77064.1"
                     /translation="MNIKIVDLGNKRFDDAYQYQLKKVDEVFNKKNEPDFIGYLILLE
                     HPHVYTLGKHGKEDNLLISPQLLEKINAELVKTDRGGDITYHGPGQIVGYPIIDLMKL
                     KIGPKQYVYNVEEVIINVLDKYGIKGDRMSGSIGVWLDPNTPKARKICSIGIRITRNI
                     TMHGFAFNVNTDLKYFSYINPCGITGKGVTSIAKELGYEVDINEVKKYILDIFSEIFK
                     VEIQNL"
     gene            complement(46268..47179)
                     /locus_tag="PKI65_01085"
     CDS             complement(46268..47179)
                     /locus_tag="PKI65_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HOB77065.1"
                     /translation="MHIYYSFDEDFEISNPVVTIGTFDGVHAGHRAILKRLNILSQKV
                     NGESVLITFYPHPRKVLYPDTLGKNLKMICTQNEKIHLLQETKLNHLFIIHFTNEFSK
                     ITAEQFVRNFLVNKLHARVIVVGFNHHFGYDKQGDYEYLYELQQYFGFQVEEIPQQEI
                     EKETVSSTRIRQALSQGLIQKANAYLQSHFPFFGVVTEINPLDNKLTKIKIKTDEIEK
                     IVPPIGLYAGVLQMDIKKIKCLIITNEIDENIIPFIEIIFQGEINITLGDNVRAFFAK
                     ELQLYDKIDNLHKSINEYITLINELIY"
     gene            complement(47249..47869)
                     /locus_tag="PKI65_01090"
     CDS             complement(47249..47869)
                     /locus_tag="PKI65_01090"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOB77066.1"
                     /translation="MILPEKTIERLSKYRRVLINLNFQQKDYIYSHELANLIHITPEQ
                     VRRDMMLIGHSGTLRRGYEVKQLIEKINSIIDGNTVQYVTLVGVGNLGRAMLNYLKNK
                     NKYLQIIACFDNDQDKINKMCCGIPCYSINEAEEQIKKLNINIAILTVPTNVANEVAD
                     ILIKAGIKGLLNFTTMPLKVPSNVYLEEYDMITSLEKVAYFTQFIE"
     gene            complement(47903..49054)
                     /locus_tag="PKI65_01095"
     CDS             complement(47903..49054)
                     /locus_tag="PKI65_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOB77067.1"
                     /translation="MIYNILIVDDEEGIRMAIKRALEKHRIDFPFLDEPIKLSLYEAE
                     SAERALEILSDTHMDIILLDNKLPGMQGIDIMPYLNEHYPDTKILIITSFASLDIALE
                     ATKHGAFDFIPKPFTPQELKNAIEIASKQIYLNSIIKDMDKEARKIRFKFLSILSHEL
                     KTPITTLEGYIYMIKEKQLGDNIDNYMELIDKMLNKLEEMRTLIMDLLDFTNLQAEST
                     IGEKELLSLKEIIMFSIDSQRPLAIQKSITFDIDIKSPLNIFASKNEMEILFNNLINN
                     AIKYNKNNGKIIIKGEENDNNIIIKIKDTGIGINENDLPHIFDEFYRTVDAKSRRLSG
                     TGLGLSIVKKMIELYNGTIEVESKVNEYTEFTIILPKIEDNKTKNFIDN"
     gene            complement(49060..49455)
                     /locus_tag="PKI65_01100"
     CDS             complement(49060..49455)
                     /locus_tag="PKI65_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOB77068.1"
                     /translation="MEKIKILLVDDDPDFSDQMLFYLNKFGYNTIYLPSQRSAESRLK
                     DLKFDLAIIDLMMEEEDSGLQLARKIKSNYPDIPIILLTAITNETGYSLSNEFDNSPE
                     LMPIDKYCEKGIRPEVLNKIIQQLLTNKK"
     gene            complement(49536..51533)
                     /locus_tag="PKI65_01105"
     CDS             complement(49536..51533)
                     /locus_tag="PKI65_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOB77069.1"
                     /translation="METRKLEYHQLVFTVQNRCKVCYTCVRECPAKAIRIFNGQADIL
                     PDRCITCGNCIKVCSQGAKDFVKHSDEVDELLQTNEKVAALVAPSFPSEFSDFKTHNN
                     FVGLLRKLGFDYIYEVAFGADLVAHTFKKLIKANPDMQYITSDCPAIVKFVKKYYPEL
                     TENLLPVCSPMNAMNRVVKNMFDYNIKTIFIGPCIAKKDDWEEVDFKITYRELRSLIQ
                     KHNIEIENIEPSTFDPPYGGLGAIFPVSRGLIQAVDIDDKLYKSNIIAAEGREDFQEA
                     LKEFYSKTWGKENLELLCCDGCIMGPGTTSLSNRFAKEAFVTNYVKRKLKNIDWEEWE
                     NNLNYWMETLDLSVEYYPSPIEDLIPSEEEIRSVLIELGKSTPEDELNCGACGYNTCR
                     EHAIAIIKGIAETEMCLPYTIDRLHSLLEELNISNKELRDAQEALKKTEKLATMGQLS
                     AGIAHELNNPLGVIIMYANILYEECPPDDPKRKDLEMIVSQAERCRQIVKGLLNFARK
                     NQVVYEKINIQNLFDKAINSVIIPEHITVKTKVDPPDLEGYIDIEQMLQAITNLIQNS
                     IDAIGNKKGEIILEAQTKDNYLAISVYDNGVGVPDSDLSKIFEPFFTTKPVGKGTGLG
                     LATVYGIIKMHRGKIEVESNADPSKGPTYTKFYIFIPQSKN"
     gene            complement(51515..53098)
                     /locus_tag="PKI65_01110"
     CDS             complement(51515..53098)
                     /locus_tag="PKI65_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOB77070.1"
                     /translation="MKINITVNNQKYKVDLGENLLNALLSLGYDIPHLCYLENYSPNY
                     ACRLCMVEIDGFNNPIQACTVDVNTPLQVYTHTPKLINIRKNILELILSQHSLECNTC
                     PKAINCSLLNYAYQYGVNEIIWRHVITDSKEKDISSPVIAKNINKCISCGICVKICNE
                     MMHCNVFNHAFIGTKKTIISSLDKPINLSNCYYCGQCSYHCPTSALYEKSYIDNVEID
                     PQNTTLIFDSAAVYNLMSQINSNFTKNPILSFNYLCQFLGFKKLINLGELIDAKLYLD
                     FQLLKDRKHPIISNLCPVSIEYIKQKYPELIEFISPLASPMQAAIRLTNKSNPILILT
                     NCSAYKNDIFQNYSNTEHKIYVANVRDFLKYLLRNGYTLEQLKNLIPNIKAKKNYSYS
                     IGGLYADISMYNLLKLILDETNIQIDKPSKINSFLTGKNENKKYNFRLKNKNYSLISI
                     NTISEIANFFDHEFLKTNLALIGCWSCANGCINGGGKTFEIFKTTNKSLSSRNPLLTQ
                     IYNINEDLIIQEKLWKQEN"
     gene            complement(53095..54966)
                     /locus_tag="PKI65_01115"
     CDS             complement(53095..54966)
                     /locus_tag="PKI65_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOB77071.1"
                     /translation="MSTIKLQLGYAITSLELQNIWEKELLSLLSQDKHFILKNHSTDL
                     SPNDDAIIIETFPNTGLKGFLYAEPLVWIKHPLKRTLCFRNLEPELIPWIIECVVNLD
                     ITSLKPWIQLDDYGESWPHIPIITQMPWYDKIVPFLTNSFSGDDWIIDNTILDNTNID
                     FIVSLYNKKSSVEVKDLIFKSKLIGRGSGTLPIEIKWQAIDEQLAEKKYLIINANEFG
                     PSSLSNYFLLRYYPTMILEGIITTCYALHINNAIIAIHQDYLDVYEAFSKLISQAKDL
                     GLLGDDILNTGNSLSIDVMLTPGMLITGEETALIHLLESQMPFPTNKPPYPTSLGLWG
                     QPTLVHNVETIANISQIIKTDKSLEIYAPISKQGTKVVTLCGEINNYGVYEISMDISI
                     DEVINKIGGGLNNSNKIKGFHIGGVTGRFFDLDNLDKSLYDLEKQTNYLGTSTIFTFS
                     EEQCIVKLLQHISEILTEQSCGKCIPCLYGSRVWSELISYLSLANNPQIPHFPLYRLK
                     TILSFNKLDRTVTESSLCALGKSLSQTFISAYNFFKDEIEEHIFDKYCRSYQCVGLRN
                     YIIDEDACTGCGLCIVECPVNAIIEKRINLFVILDGKCVGCGLCEKVCKFSAINLKK"
     gene            complement(54963..55403)
                     /locus_tag="PKI65_01120"
     CDS             complement(54963..55403)
                     /locus_tag="PKI65_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77072.1"
                     /translation="MRIILPFTINELFKEVWHVYPNSLIDFCAKIMLEPYALSKSHFD
                     ELEIYFKQPASEIYAFVVTCFPDLLISDINYDIVMCRGWNCYLKYGKNFLNEVNYQFR
                     NESEKPIIKFHKCNGKCHTDQIELNINSAFYKILNNIKEKIIKK"
     gene            55728..57500
                     /locus_tag="PKI65_01125"
     CDS             55728..57500
                     /locus_tag="PKI65_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB77073.1"
                     /translation="MKYSKKIKSTDIIIDEKKQFINKYNFVIIPILLIFLSFSLSNCK
                     IKDTNKTLQASVEIQNLLLDAQKCLLLNELDQAKEILFDIISKDPKQDVAYYLLSKYY
                     ELNNKWDSANKYIDIAIKLSPENVYYLELSANILVKLRKYREAAQRYEILISKNPKIL
                     DYYLNAAQMYLIAFMPNNALEIFTKLESQHGFIPEIGFYKYQIYSLLGKSDEALDELW
                     RCIQYDPSNLDYKKEYSSFAIEKGKITALYRLVQWYKQRDSTDGYAALFAANYYFAIN
                     KVEELKKELLIAIKDPRVDFQDKLQVILTSIENPNIDEQFIQQLFYHLEDLHSDKAET
                     FNIIGFYYQAQNILDTAIVYFKKALELDSTNDATYRNLIMSYEAKNDYDSMLFYANKA
                     LNFYPDIGEFYFYAGYAAFMQKKFEKCIPHLENALSYIPSKNKELQNIIIELLANAYY
                     ETGEYAKAFDYFEKAIKANPANLVLKNNYAYFLAQQDTMMDKALNLIDEVLLTEPTRF
                     SFIDTKAWILFKMKNYDEAKKWIDKALMYNDSDPEILEHAGDIYYHLNLIDDAVQLWQ
                     KSKENGNNSEIINKKINDKKYYAF"
     gene            57490..58290
                     /locus_tag="PKI65_01130"
     CDS             57490..58290
                     /locus_tag="PKI65_01130"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOB77074.1"
                     /translation="MRFKFILSILIVSLVFFSCKTSKSPQQYSQDSKFISIESKDTFV
                     LVNNTSYNNISLKTNGIFNYENKEINFNANIIIIHDSLVWMSASVLPGIEIFRILVNK
                     DSIKIINRFSREYYTDSFEKIYEKIGICINFDVVEALLVANDFISFNHEIIQKHTDSL
                     FTNYTISRNIIIGPDDKNCNLLQNLKISNNSLKIVQNELNYWNRDNISISIKYEKYDL
                     IGTMLLPTNINIELKSYENPLIIAKVLFKQIKLNQNVSTPFNVPNWGK"
     gene            complement(58385..59623)
                     /locus_tag="PKI65_01135"
     CDS             complement(58385..59623)
                     /locus_tag="PKI65_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOB77075.1"
                     /translation="MKSSLLSYWIAYRFWKSKKSYSLINILTYVTFFGIMIAVAALII
                     VICVFNGFEDVIVSMTEVIYPDISISKADGKPFDLNELDLNYIKQINGIASIEPTLEA
                     TALARYDDEQLVVKVKGINPNGYNSKNIAPYLLYGEPQVILYDSIPAAILGSGVAHNL
                     NVNIKDYSKYLYIYSIKESSSQVSATTLFTESACMISGIFAINQDYDNIYIYAPIDEV
                     QYLLGIANKFSNIEIGISDKSNINKVKKELANLLPEGFIVKDRHEQDELLFKILRNER
                     LIVFILLAFIVLIASLNIISSLGLLLLDKQKRVQTLYALGMNISQLKKLFLREGAMIV
                     LGGGAIGLLLGSIIAFLQLQFGIVPMHGGDMVGHIAYPIAFKVRDYFFIILLLLSIAI
                     LLPRIPIKRIDKDFVAIRNV"
     gene            complement(59623..59961)
                     /gene="rbfA"
                     /locus_tag="PKI65_01140"
     CDS             complement(59623..59961)
                     /gene="rbfA"
                     /locus_tag="PKI65_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOB77076.1"
                     /translation="METIKQQRVASVIKRALSEIFVNEAKEWFGDAMITVTVVRVTAD
                     LKLAKVYLSIYGIADKNEVLKEIQILKPQIKNLMVQKIRHQLRWMPDLQFFIDDSLDY
                     IEKIDNILNK"
     gene            complement(60161..60958)
                     /locus_tag="PKI65_01145"
     CDS             complement(60161..60958)
                     /locus_tag="PKI65_01145"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOB77077.1"
                     /translation="MSKNNKNCKTKSTDNFIEKKQINPKTSIKTKIGSLFFHYRNYIF
                     PFFYFILFIPSPKIFPSFTIPLILGLILLFIGLGIRYFTIGLVNINRAGKNKYIYADK
                     LHTDGLFSLCRNPLYLGNIIEICGLAFLSNSLIFLLIMFPLFVFIYYCIVLAEEDFLL
                     KKFGNEYLEYKQNVNRWIPNMKNISKIINSTQFNYKRVLIKEYTTTFIALLAATLLIM
                     KMYYVFYLNNFINSLPYFISIIFVLIFTYFAIRYLKKTKKITAKLFL"
BASE COUNT        21702 a         8806 c         8695 g        22006 t
ORIGIN      
        1 ttttagcact ataaaaataa ataataaaat ttttattttt gtaaaaaatt tttatttttg
       61 taaaaaattt ttatgaatag tgatatccgt actcgtgatg ctattagagt atcatggatt
      121 ggtttttttg taaatcttat tctttgcttt tttaaactat ttgcgggtat atttggcaaa
      181 agtactgcta tgctagctga tgctattcat tctatttcag attttgttac agatttaata
      241 gttatagttt ttgttagtat ttcagcaaag cagcgtgatg caacacatga ttacggacat
      301 ggcaaattcg aaacttttgc ttctttatta gtaagtgcta ttttgttagc agtgggcata
      361 ggtattttaa tcaatggtat taataaagtt tcatatgtaa ttaagggcaa atctattgct
      421 gctcctggta tgatagcatt tatagctgct attgtatcta tcatcactaa agagtctttg
      481 tattggtaca ctataaaaat agggaaaaaa acaaataatc aagcagtgat agctaatgca
      541 tggcaccacc gttcagatgc tttatcatca gtaggaacag ctttaggtat tggtggagca
      601 atatttttag gcgaaaaagc tcacatactt gatcctatag ctggtatatt ggtaagcgga
      661 tttattatat gggctgcttt gaaaatttta ttacctgcta tcaatgaact attagaatca
      721 tcgcttcccg aaaatttaga aaatgaaatt gtcgatatta tcaaatcaca tccagacgtt
      781 aaagattgtc ataatttaaa aactagaaaa attggtaata tttatgcaat agatgttcat
      841 gtgaaattag ataaaaatat tacttttgtt cattcgcatg atgtagccag tgagatagaa
      901 agtaaaatac gtaataaatt tggcaaagaa accattataa atattcatac agaaccaatt
      961 tgaggtaatt tataacccta cattttaatg atttacaaat ctaatactca cgatctagag
     1021 tatactaaat taacataatt attagaaggg atcaattaaa aaatttatgt ttataagttt
     1081 ttgtacgttg taaattttat aaattctgca aaattggtat taaaaatcaa tcttctagtt
     1141 cgctaaattc tgatttatca gcgccacata taggacacac ccaatcatca ggtaattctt
     1201 caaaaggcgt acctggtgct ataccagaat ctggatcacc tttttgtgga tcataaatat
     1261 aaccacaaat atcacattca tactttttca tatcttttta ttttttgaca tgcaaaatta
     1321 aaaattaatt tattaattta caaagctgtg tatccaccat ctattactaa ttcagaacca
     1381 gtaacgtagg aagcatcatc agaagctaaa aacaatgcac cgtaagctac ttctataggt
     1441 tcagcagctc ttcctaatac agtggtgcca agcagatttt tagcattttc tggatttttt
     1501 aataaatcat cagtcatagg ggttttaatg acacctgggt gaattgagtt gacacgaata
     1561 ttatatttag ctaattcggc agcaactgtt tttgtcaaaa gcctcacagc gcctttagaa
     1621 gcatgatatg ctacggctgc accactacca accaaaccat aaatagaaga gatattaata
     1681 atagatccat ttttagtcaa tttcatagca ggcaatacat acttaatacc caaaaaagtt
     1741 cctttcgcat taatttccat aattttatcc cattcctttt cagtgatttc ttctatacca
     1801 cacaaattta atataccagc attatttacc aatatatcta ctttgcccca tctgtccata
     1861 atttggtcaa ttaatttttc ccagtcttta gctttagcta catctagttt aaatgctatt
     1921 gcttcgccat tattttttat tatttcatta gcaactattt tagcctcatc tattagaata
     1981 tctgttacaa caattttagc tccttcgtta gcaaataaaa tagcttcctc tctccccata
     2041 cctttagcag ctccagttat tatcgcgatt ttattttcta attttttcat attacctaaa
     2101 ttatttgatt tatttataca aaattaaaac atattcaaca taaatatgta taaaatgaca
     2161 aatatcatat atatttatgc gcaaatatat ttcataattt aagttatccc aatagtaagg
     2221 agctatataa caaagtaatt attaggaaat tacgagagca aaataaggca tagtaaggtt
     2281 tataaatttt aatataatat ttcaatgtat gtgataaaaa aaggagttat ttatgataaa
     2341 taactccttt tataaatttt aatatatatt ttttttaatt tttttctagc aattctattt
     2401 tttgatttat ttcttttaat ttatcaggca tagacaatct agcatatata gttctgagtg
     2461 tgtatagagt agggatatca tctggttgaa tagttagtgc tttttctaat aaaggtaatg
     2521 attgatgcca aagagattca tatacttttt tcaaattttc atattcagct tgttctgttg
     2581 gaggcagctt attagcttta ttgaaaatct ctacgccttt attaaatagc aatgcaccat
     2641 agttgtaata tgcttcataa aaatcaggat ttaactcaat agctttttga tatgattttt
     2701 tagcttgttc gaaaaataaa tcccttctct cttgagataa agtagtatct tgggccagtt
     2761 gatcataatt agtacctatt acataataca acataggatt attaggatct ttagtgatgg
     2821 cttcttgcaa tagtaattga gatttttcta aatcaccagt aattagataa taatttattt
     2881 ctctcaataa aatatcatta tcattaggaa atcttttttt acctaattga attgttttaa
     2941 gcatattatc agtatctttt ttagctaaat aaatatcaaa taatagagta aaaactcttg
     3001 gttcattata attagtagca gtagttagct cctttaaaag tttttcagct gtaatagtat
     3061 cttgtagatt aagagctgct atagcaccat aaaaattggc taaagtatca acctcttttg
     3121 taaaaatttt tcttgtattt cgagactttt cgaaataaac taatgcatta ttatagtctt
     3181 tattattgaa atatgtagcc cctatattaa agtattcatc agctattaat ttgagtccca
     3241 tattaggatg tggcggtaca gcagttgata ctgtaaaatc tgggtctaaa atgagacatt
     3301 ttttatatgc ttctaaggct gtatcgagag ggttaggagt cagctgttta tatttatcat
     3361 ctttagtaaa agcaatagcc aagtaaacat tgcctttgta gagccaagtt ttaggatcat
     3421 ctttagtatc tggatattga gccgcagcat ctatagctgc tttagcttta tctaattttc
     3481 ctgcttttaa ataattataa gcatctaata ctttatttga ttgagcaaaa ctactcacgc
     3541 ttattaaaag tatcatactt attaaaaaac ttttagtttt catactgtta attttttata
     3601 caaatttaat acaaattttt taaatatttg tgaatttttc tattcattat tttcttctaa
     3661 gctaatttcg gcttctatat tttctgtatc atcatcatca atgtcatgag ctattttggc
     3721 aacagaggca atagcatcat cattttgtaa ttgaatcaaa cgtgaccctt gagttactct
     3781 attttgtaat cggatattag aaatattaag acgtatacct atgccatttt tagtgatcag
     3841 cataatgtca tcattttcat tagttacatc aattatactg atcagttttc cagttttttc
     3901 agttatgttc atagctttta tacctttagt agcacgatga gtttttcgat aagctgataa
     3961 aaatgaacgt tttccataac ctttttcaga aatcaccaat atttgttgat tttcatcttc
     4021 cattactacc atacctatca cactatcatc tttagaactt aaagttatag ctttaacacc
     4081 tatcgcagtt ctacccatcg gacgtacttc ttcttcatta aaacgtacag ttttaccttg
     4141 actagtagca actactattt ctgatttacc atgagtaagg gctacttgta ataaatcatc
     4201 accatctcta atattcaaag ctattatacc agtactgcga gggcgtgcaa aagctgtaag
     4261 acttgttttt ttgataattc ctttagtagt tataaaaatc acataatcat ttttaatagc
     4321 atcatggtca tcaagatttt tagtattaat aatagtttgt attttatcat cactatctaa
     4381 ggctattaga ttttgtattg gctttccttt agcattgcga gcagcttcgg gtatttcgaa
     4441 tacacgtaac cagtaaactt ttccttttcg agtaaataat agtaaataat catgattagt
     4501 agctatatac aaattttcta taaaatcttc atctctgata tcagatgcta tcttgccttt
     4561 accacctcta ttttgactac gataatcact taatttagtt cgtttgatat aaccaagatg
     4621 agatattgtg ataactactg gttcgtcagc tatcaaatct tcaattctaa catttttacc
     4681 atgaatatct atatctgttc taggtttatc accatatttt tgtttgattt ctagtagttc
     4741 atcttttatt atttgcattt gcaagttttc gtcactcaag acatgtttga ggtaagcaat
     4801 ttttttcatc aactcatcgt attcactttg caatttatca cgctcgagtc cagtcaaagt
     4861 tctcaatctc atatcaataa tagctgtagc ttgtatatct gtgaaatcaa aacgttgcat
     4921 caaaccattt ttagcaattt ctactgtttc actactacga ataatggcta tcacttcatc
     4981 gataaaatcc aatgctttca ataagccttc taagatatgt gctctttttt cagcttcatt
     5041 taattcatat tgagatctac gaattatgac ttcatgcctg tgttcaacgt aataatggat
     5101 taattgaatt agattcaatg tttcaggctt accatttacg agtgctatat tattgatact
     5161 aaaagtagat tgtaatggac taaatctata aagtgaattt aaaataacat tagcattagc
     5221 atctctacgc atgtctatca caatacgtat gccttctcta tcagattcgt cccgtatatc
     5281 agtaatccca tcaatttttt tagattctac cagtgcagcc attttttgca ctaaatcact
     5341 tttattaact tgataaggaa tactagaaat aataatttga tgtttattat tatgttctat
     5401 gatttctgct tcaccgcgta tgcatactct acctctacct gtattataag cttctatgac
     5461 gccatcaatt ccataaataa gtccaccagt aggaaaatca ggacctttta tataattcat
     5521 taattcctct acagtaatat ctctattatc tatatatgca atgatgccat ccactacttc
     5581 ggataaatta tgaggaggca tattagtagc catacctaca gctatccctg aggctccatt
     5641 aagtaacaga ttgggtatct tggcaggtaa aactgtaggt tcttttaagg aatcatcgaa
     5701 attattttga aaatctacag tatctttttc taaatcagct aacatttctt cggcaagctt
     5761 agtaagtttg gcttctgtat aacgcatagc tgcagggcta tcaccgtcta tagacccaaa
     5821 attaccttgc ccctctacta atggatatcg catagaccac ggttgagcta gacgtaccat
     5881 agcatcatat acagatgaat caccatgagg gtgatattta cctaatactt ctcctactat
     5941 acgagcactt tttttggttg gtctattaga aaaaagacct aattcgtaca tgccatacaa
     6001 tactcttcta tgtacaggtt ttagtccatc acgtacatct gggagagccc gagaaactat
     6061 cacagacatt gagtagttaa tgtaggcagt tttcatctga tcttcgatat ttatatcaat
     6121 tatctgccct ccactttgag ttaattcatt gtcttccata atatagtaaa attatttgca
     6181 aatgtatgaa aaatatatca aaaagcaaaa gtaaatttaa ttataaagaa aataaatttt
     6241 tagcattcat agttgtttta aatgctactt tttttatatc tattttaaga atttcagcaa
     6301 ttttcttagc aatatgtggt ataaaggagc tttcatttct tttacctcta tatggtactg
     6361 gtgttagata tggtgcatct gtctctaaaa ctaaataatc taagccaatt tgtgatatag
     6421 tttgtgccaa tttattattt ttaaaggtaa taataccacc tattcctagc ataaaaccgc
     6481 gatcaataat tattttagcc tcttcataag ttcctgtaaa acaatgaaat ataccccttg
     6541 gtttattaga aaactcatct aatatagaaa taatttcatg taatgaattt ctctgatgaa
     6601 tgcaaactgg taagtcatat tgtatagccc attgtaattg ctgtataagg actatccttt
     6661 gctctactat gtaagtttta gcccaatata aatccattcc tatttctcca ataccaatga
     6721 aactatttac attattttgt aattgatttt ctataaaatt taattctaat ttataatctt
     6781 cttttacact accaggatgc aatcctatca tcattttttg taaatttaca tttacttgat
     6841 agcactgcca catagcatca aaagtgctca tatctatggc tggaagtata attaaatcta
     6901 tgcctgcttt ttcagccctt tctaaaacgt cttttctatc tttatcgaaa tcctctaagt
     6961 atatatgcgc atgagtatct atgtaattta ttgcttttcc catgtataaa aatatatttg
     7021 attaccagaa tttatttgaa aaatataaat acctgatgat aagtgatcta tttggatggc
     7081 cactttatta ttatgctttt cgataatagg acaatctatt ttagctccta gcaaactata
     7141 aattgataat ttttcgatat ttatatcacc ttttatatat atgtgatcat tggcaggatt
     7201 aggatagatg atccatggct tgttattttg attaaaaatt gatatttgat caaccactat
     7261 atatctaaat ccatcactaa aagtctgaat actcatacct gcattattaa aagttttaac
     7321 tgtaatataa taaaaattat cataaattaa acttgttgga ttaaatgaat aaaaattatt
     7381 agttcccaca tttatccaat cgactatatc attataacca ggttgagaac ctagacaaac
     7441 ttcataatga ctaatgccac tattaggatc ttgggcatgg tcccattgag tagatatcgg
     7501 taataaaaaa atggtatcat aatcttgtcc tatgccatcc caaacataag aaggcggtat
     7561 aggaggagta taatctacat atacatcaca catagcagta ttcgaaaggt tatctgcatt
     7621 atcttggaca atgcttttga tcttgccagc aggtgacaaa gtattgatac tttgatatct
     7681 caattcctta gaattatcgg gacctacacc tatatgtatt gttgcatttt cgtaacgaga
     7741 acgatagacc ttgatttgat taaattgtgc tgaacaattg cctgttctga aagatatata
     7801 actaccatta ttataaggtt gactatctat ccatttacct tttaataaat tattgacata
     7861 aacccatatc ccaccatcta ttctatcata tattactttt atattgtacc attggtttaa
     7921 gtttaatgga cagttagttt catgtacttt agagaaaaca tcattcgtta ctttataaaa
     7981 ttcgagtttt ccagtttcta atctaaacca gataaaatat ccattacctc tattttcaga
     8041 agcagagtca gaagagaaaa aataaaagcc tacacgttta tttgtcaaat tattccctag
     8101 tacttttgct tcaaattcat ataaatatct attagaaagt tcttgatcga ggtaagtgct
     8161 gatatgagta ttatgaatag acgaatctgt ttgtatgaga caaccatttt cttgtaccca
     8221 atttcctgtt atgatgttcc atctactatt tatggatggt gaatcgaaat tatcacccaa
     8281 gaagcctcta tggggattgg ctccccaatc tataccgtcg tattcgatca cttgataata
     8341 agccttttgt ataccactct cagcatcaga atcttgaaat ataactgaga aatcttgtgt
     8401 ttgccaagta gatattgggg agataagtgt aattggtggt atattgtcta ttggagactg
     8461 atcacattga taatatagtt tgaaaccaga tttattaata gaagagttag aatgaaactt
     8521 tagagtaaag gcaccagaat tagcaataag agtgcctgga agagaattac ctgtaaacga
     8581 ccctatcaat tgagaagatg tagaactacc atcatacaac cacaacatat cattattagg
     8641 ctctaaatct aattgagtaa aattaagtgt aatagattga gcatttgatg gtgaaatagt
     8701 gtatgtataa tcttcattat cataataatt tctaagaggt ccacccatat cgaataagct
     8761 atctacacaa ggcgtcagag cacaagtagt aaaatgatct ctaatagcat cccaaaaatc
     8821 tgaataacca tcatcattac ccagagccca tattcccata cctgctatac ctctttgatt
     8881 aatcaaatcc atgcgcttac ccatactata tttatcatct atgaaacatt ggcgccactc
     8941 agtaccatca taaaagatat atgcgggtgt gaatgaggcc tgatcccaca gtttgttagt
     9001 agcagagtaa tgtcctgttg tgttgtctct aacaaattta tatgtgcgcg atacacaatt
     9061 aggaggatta tttacatcag aaggtatagt attggataca gtgccgtatt cacgtccata
     9121 ataaggtaaa ccgagaatta atttattagg tggacaacct ttatttagat aatgagtaat
     9181 agatttagta agtgtataat tatatgaggt tacaaaatta tataaagggt ctgtagggcc
     9241 tgcagtagtg ctaccacccc aataataatc gtaacccata atgacgaaat aatctacttt
     9301 atttttcatg gcattaatgt tgaatacatc actccattcg actgcattaa gagctatagt
     9361 aacttctgat cctgggatac gtgtatgaaa agtatcagca agttcgttca taaaattagc
     9421 aaaagcagca ttatgagatg agttcattcc ttcgaaatct atatttactc catgagctcc
     9481 tctatttaaa actaaggtta cgatctgatg cataaaattt ctttgtgctg taatattatt
     9541 taaaaatgtg gaatgattgc taaatagagt agcgcataga tgtaccttga cacctgcatt
     9601 tagagcgtct gtaactgatt gagcagtgct aaaagcttga gtagtagaag gatttccagt
     9661 ggcagggtct attgcatagg caaaatatac aaaatgactt aagagattcc aatcatagtt
     9721 tgtttgtaag ccattttgcc aataaggatg ccatccatag accaacttag tcaaaacaca
     9781 attatctttt ttacgaggcg ccatattagc tttattcatt agattgtaat gctcataatc
     9841 ttctattgtc atccctgtgg attcgtgata gtaaaattct tcttgtacta ttgatatttg
     9901 cccatttaaa aaattaattg cattagcaaa taatagaata gataaaaaaa agggtattac
     9961 ttttgttctc atatttatat atttttattt attacaaaaa tataaattta aaaatttcat
    10021 caaaaatagt aaattaatct aatttaaaag ttaaaacttg ggcatttaaa tatcataaaa
    10081 tcaattcgct aagattaatt taaataaatt gaaaattaat aaatcactag tatacttaat
    10141 tatttgattt tcatttcaat tacttgttat ctattctatt tttaaagctt ctgcaggtga
    10201 tatctgcttg ataaccaagc gaggtatcac tattcctaat aaacctaaaa atataatagc
    10261 taaattggca aaaatgaaat aatcaaaatc aaactgcata ggcacatatg acatgtagta
    10321 catagcttca ttgagtggta tcaggtgcca tagattttga gcaattatca ataaaattgt
    10381 aactacatta ccaatgatca aactataaca tatataccgc attgtaatcc acaaaaatac
    10441 ttttgaaaga ttatttgtgg tcattcccag agtcttcatt agtccgataa atttgatttt
    10501 ttctaagatt aatattagta aaatgctaat aatattaata aaagcgacga tagccattag
    10561 aatatatatc actaccacat tactatttag catttctaac catgaaaaaa aacgattttc
    10621 tttgtctctg acgtctatta attgcacatc ataatttagc ctttgcaata tcaatggttt
    10681 tataatatct attttatcga tatctgtcag atatatttcg taagctgcta ctaatgaatc
    10741 actccaacca tctatctgtt gaggaatttt aatattacct aaaacgtaat gattgtcata
    10801 atcttgtaat ccggtattat aaataccaac aatttttaat ttacgacctc ttggagtgta
    10861 ttgaccttct ttaaaaaaat aaattctaac agcactatct atgtctagat tatacttttg
    10921 ggcaatattt ttagaaatac atacttcagt gagggctgta tctaaattag ggaagcgacc
    10981 ttttacaaga tttttttcga agaaatcttt tgggtaatca gcattaatac ctttaaagat
    11041 gatcccttcg attaaattat cttcgtgtag aattgctggt ttttctatta caggtgctat
    11101 agagacgata ccactaatag atttcaaatt ttctacatca tcgcgtggat atgcaatagg
    11161 atcgctagta taaacttcta ctaaactact ataattagca atttgtagat gaccattaaa
    11221 accgactact ttttctttaa tttcagtttt gaatcctcta atgatagcaa aagaaataag
    11281 catagcaaaa atactaatag ctattgatat ttgagcaatt atcagtatag ggcgcgctga
    11341 cccttcttgt ttactttgta tcaatttaga agcaagatac ctatcgattt tcaaattttt
    11401 ttgtaacaaa agtaattaaa aagaattaat tattattaaa ataaatataa ttaatttttg
    11461 atttgtaatt ttggcaataa ttttatgtta attttttgaa attatgataa ttaaattaga
    11521 aaacatttat aaatccttta agcctgttga agttttgaaa ggtatcaatt tagaaataaa
    11581 agaaaatgaa tttatatcga tagtaggacc tagcggcgct ggcaagtcta cattgctaca
    11641 tttaattggc acattagaaa aaccggatag tggcacagtg aaatataatg atatagatat
    11701 ttataaatta aatgataaag aattgagtga ttttcgtaat aaaaatcttg ggtttgtatt
    11761 tcagtttcat catcttttac cggagtttac agctatcgaa aatgtaatgc ttccagtatt
    11821 aataggtggt ctaaacaaag atgaagctat ggataaagct aaaatattat tggaaaattt
    11881 aaatttatca catcgcttta ctcatagacc acaagaacta tcaggaggcg aacaacaacg
    11941 tgtagctatt gctagagctt tgatcaacaa tcctaaagtt ttacttgctg atgagcctac
    12001 tggtaatcta gatagtaaaa atgctcaaga agtaataaat ttattcaaga aattgcgtga
    12061 tgaatgcaaa cgcacaataa ttttagtaac ccaccaagag tcttttgcaa aaatatcaga
    12121 tagaatagta tatataaaag atggagttat tgagtaatta tgaaattttt cacatttaaa
    12181 tgattaaata tcaattttaa taactcaata tcacaaattc agtccttcta ttcattgctc
    12241 taccctcttc agtgtcattt gtggctattg gtctttgttt accaaaacct ttatattcca
    12301 aacgattttt atttattcca ttaataacta aatagtcata aactgccctt gctctttttt
    12361 cggaaagtat caagttgaaa tcgtcgctac cgatatagtc cgtatgtcct tcgatgctaa
    12421 ttttcatagt agggtgatca ttcaaaaata ttataaactc atcgagtacc ttttgagaac
    12481 taggtaaaat atctgcagaa ttaaaagcaa aataaatatt gtgtaagcga tagtgcattc
    12541 ccagctctat aggttgtact tccatttcta gctctataat tgaagcctct aatgaatctc
    12601 tagcagatat ataagtactc tgataaacaa tatcttcgcc ttttatagtt aataaataat
    12661 cttcattttt taacaatgta gctgcataat ctccctcatc atctacagga ataaacgata
    12721 cttttttgct ttccatgctt tttatctgta tagtagcttt tacagactgt gtagtatctt
    12781 gagattttag atttcctttt accaacaata cttcttctgg tttagcatca ggatgcaaat
    12841 cgaaataata aaaatccatt ccaccaatgc tttgattttt aatattatta ctagcaaaat
    12901 agccatatct accatctaga ctcactacga aacccacttc atcattttcg gtatttattg
    12961 gaaaccctat atttttgact ttcagataag atttatcatc attatcattc aattttacat
    13021 aaaaaatatc ataaccgcct attccaggat gtccattaga actaaaatat aaggtacgag
    13081 tatcagtatg aataaatggc gatttttcat caccaggagt attaataggt ctgcccaaat
    13141 taataggttc agaccattca ccattttgat ctcttatact tttataaata tcataaccac
    13201 ctatacctcc aggtctattg ctcacaaaat ataactcttg accatcagca gttattgatg
    13261 gcatactttc ccaagtatct ggcctattaa ctctatctag tggtatgata tctgtccaat
    13321 aacctttcac gtatctcgat gaatataaat cgcaattata atactgtctg ggactattga
    13381 ccattataca tacagtatag atcaattctt tgttattacc tgtaatcgta gcaccgcctt
    13441 cattttcatt tctattaaaa ggatatggca ttagtttgcc attatcaaac tcatttttac
    13501 caatacgata actatagtaa aatctttctt gaaatgcagg ctcagactta taaccggata
    13561 attgatattc ttttgttttt ctagtataat aggctatttc attatcagga ctcaaagaca
    13621 ctaaatagtc atctaaagga ctactaatac cttttactaa tttaggctca aaaggaacgg
    13681 gattactgat tatgtcacat atctctatac tccacttcaa aagcaatcga gcttcatcca
    13741 aaagagtatc aaaatcacct tcataagata aaaaacgctt aaaataattt atagcctcac
    13801 atttatcatt attagtatat gctaatttac ctaaatgaaa atatgcctct ggtatatcat
    13861 caggacaagc atctattgct ttttttaaat attgttctgc aaggtaaaaa ttattatcag
    13921 gtttataaat atatgttaat cctaaataat agttaacaat aggatattca tgattttcat
    13981 ttatagattt taaaatttcg gtagcttcac taaaataaat tttttgagat tcaataaatt
    14041 tgctttgcat gccttgatat atttttgaaa tagcaaggtc caatttttgt tgatgtttgc
    14101 ttttaggaat gcttgtacaa ttgtaatctt gtgctgaaat ggataaaata ctaattaagc
    14161 ttgcaaaaaa tatataaatt tttttcattt tttcattttt tattattcaa tcataatcac
    14221 ataatgtaat gcaaatatac ataaaaaata aaattattgg aagttgtaaa ttatcaaatg
    14281 ttaattaaat gattacttta taaataaata tgaaattcaa tatcagtaca aataaattaa
    14341 aaatccaagg gcaaatatat tctaaaagta gtccctttgc ccttttcact ttctaattct
    14401 aatttaccat ttaaaagtgt gacaaattct ttaacgagta acaaacctaa tccagagcca
    14461 gtttccttat tagtaccatt ctgaactact ttttcaccga ttttaaataa tttttcttgt
    14521 aattcttcgg acattccaat acccgtatca gatacagaaa ttatcaaatt accgtttgat
    14581 atttcagtac taatagttac tgtaccttga gggtgagtaa attttatagc attatttaca
    14641 agattacgca aaatagtatt taacatatcc acatctgttt ttatatctat attagtatca
    14701 ccttcaatta ttaaatcaat atttttgttt ttagctattg atgtaaacaa atcttgcacg
    14761 tgagatatta tattatttag attagtatct actattttta cttctatttt acctgattga
    14821 gatcttaccc ataaaagcaa attgtcaagt aattgatttg tgttattcac aaccttttct
    14881 agctcatcta tcagagcatt tctctcttct tttgttagat cattaaaatc tcttctcatt
    14941 attctgataa aacttatcat actagctatc ggattgcgta tatcatgagc tatgatgctc
    15001 aaaaatttat ctttagttgc atttgctatt tttaaggctt cttcgttttc tattagtttt
    15061 ttattagtct ctattaattc taggtttatt ttagccattt ctttaactaa tctacgattt
    15121 ttcattgtat ttctaaacaa aaagtaggcg ataataatag taatgattat tatcaaaacc
    15181 ataaaaaact gtaaattgcg attatttttt ataagtttag catttatttc taatttttgc
    15241 tttaatgcca tgttttcatt aataagataa tcataagaag ttttcacaag tatatccagt
    15301 acttcttgaa taaaattttg attatatttt tctttataag cagtataaga ttttaataat
    15361 gcatcgaaag cacctttata attaccaaga caagcataag attgtgctaa aataaaattg
    15421 gctgatatct tagcatttgt tagattagca ctatctgcat attttaatgc ttctttgcta
    15481 aactcaatag acattgtata attacctaat ttataatatc catctgctat attatttaat
    15541 tcggtagata taccacgttt atcattgatt ttttttctaa attctaaagc ttttttatta
    15601 tattctattc ctttttcata ttctccaaat tcattatata agtatccaat ataagaataa
    15661 gcagtaccta ttaaattgta attatttgat tcttgggctt tttctaaaac atataatagt
    15721 atttcttcgg cttctttata tttttttaat tctataaaat ttaatgctaa attcatgtat
    15781 gaacgcaaca attgttcttt actattgata tggtatgata gttgtattgc agattgtaca
    15841 gcatctatac tttctttgta aaagcccata ttaaaatatg ctgaacctaa taatctgtaa
    15901 aattctatta ataaactatc atttttaaat cctggtagat aatctttagc ttcgaaaaag
    15961 tttgatacta ttattgaatt caaatttata ttaaaagcaa ttacactttg ataatataaa
    16021 gaatatacta tgcctacagt atcgtgtagt gaagtaaaaa tcctgtatga tgtggaaaaa
    16081 tgttttaatg attgtatatg attagaacct aagtaatgca aacaacctct agtaaattct
    16141 attttagctc taaggtttaa atcattttta tataaagctt gattttctaa agtatctaaa
    16201 atattaaata ttttaattgt atcttttatg ttttttagat tatcagttat atcatccaaa
    16261 gatcttttag ttataatatc gataaactcc ttatcgatat cataagtatt tttggaattt
    16321 aatcttggta tacataaaat acacaatata acacatagaa atatttttaa aaaatttttc
    16381 attttcaata ttatttatgc aaaaataata tttttatcga aagtaaaata tgtattatct
    16441 aaaacattaa ctgattttat ttaagattgt tttttatttt aattaaaaat tactaaagtt
    16501 taatataaaa gctataaatg ataaaatgag agataaaaaa gcaagaaagc cgaaagtcat
    16561 ttgttgttgt ttttattttt tattagcttt taagtgtatt aattatttag tattaaaatc
    16621 ttttttgtaa atataaatat atatttttgt aaaaatatta taatgttaac atcctttttt
    16681 gctagattac ttcaaatatt aataggttat actcctcatt ttttactttt tttttgggat
    16741 tattttattt atttcattac atatcatttg ctacactatc gaaaaaaagt agttcaaaaa
    16801 aatttaaaat tagcatttcc tgataaatct aaaaattatt ataaaaaaat cgaaaaacaa
    16861 ttttatcatt attttgccag attaatcata gatatagcta ttaatgggac tacaaataaa
    16921 actaaaagat taaaatatct aaaatttgaa aatataaatg aaataaaaaa atgtttatca
    16981 gaaggcaaaa atgtgatgat agcattggga catttgaatg catggacgac acaaataatt
    17041 ttatcagagg ttttagaagt gccagtctat atggtttata aaccattatc taataagtat
    17101 attgataatt ggctaagaaa taatctacat tctcccaata ctattttcat agacaatcga
    17161 tctgctgcaa ggcatatatt aaaagtttca aagcaaggca ctccatctgt ttttttaatg
    17221 gtcgcagatc aatctccagc tacagataca ggggtgcatt gggtggattt ttttggaata
    17281 aatacacctt ttcatcgagg catagaattt ttagctacta aatttaatat gccagttttt
    17341 tttgcagata tattatatat aaaacctttt gaatatacta ccaaatatca tttaatttat
    17401 aatggagaag aaaaagtaga atcaaaaacc attacacaaa gatttgcaaa tgttttagaa
    17461 aaagctattt gcgaacaccc tggagactat ttatggtcac ataaaagatg gaaaaaagta
    17521 attaaatatt gatttatttt aatattatat attaaacctt ttaaaaaaaa taagttaata
    17581 attcaatatt tatggagata ggtatagaaa aaattatttt ttaaatcaga cttttagtta
    17641 atgacattta gtaaacttat acgaagcatg atttttagat ttatgatgag tagagagttt
    17701 caatgaagag ttagaggaga atgagcaaat aaaggcattt tgaaactaat attcaagcat
    17761 ataattatta acatcttaat tttggggaac aaaaaagtga aggtgatgat tttttttggt
    17821 cctgattaat aatatttttc aaacatagat taaaagtttc gaaaatcgga aaaccattgt
    17881 atgttgtcat tttaagatca catttttctg ataaatatca attatgaaat atattaataa
    17941 acatttatac ataattttgc aatttattat agaatagata atgactttaa acgatctaga
    18001 tataggacaa agtggctaca tagttaagat caagggtaat gagacttttc gtcatagatt
    18061 aatggaaatg gggtttaaaa ccaatgaaaa tgtcactctg ctacacaaag ctccccttaa
    18121 tgatccatta ctctttcaag tcatgggctc taaggtttct ctcaggagaa gtgaagctat
    18181 taatattgaa atcacaactg aagaagaatt acaagaatct gaaaatattg atatactttc
    18241 aacaaatatt caacatttaa taaccaaaaa gagcaaaaat atagaagtag cacttatagg
    18301 gaatccaaat tctggaaaaa caactatttt taattacgtt agccacgcac atgagcatgt
    18361 gggcaattat gctggagtca ctgtagatgc caaaagtgga actatagaat ttaaagatta
    18421 caaaatcact tttgtagatt taccaggaac ttattctctt caagtatttt caccagaaga
    18481 agcctttgta cgccattatt tacttaaaaa aatgccagat gttatcatca atgtaataga
    18541 tgcttctaat atagaacgta atatgtttct tactactcag ctgatggaat tagactatcc
    18601 tatgattata gctattaata tgtatgatga attaacaaaa aaaggagcaa agctaaatat
    18661 tgataaactg gaagagatgt ttaaaatacc gattattcct actataggaa taaaaaataa
    18721 aggtatagat aaacttttga gtgcagtcat cgatagatat gaatctactt taacagtgaa
    18781 aagcgtcgta cacttccctc tgccagtaga agcctgtata gaaaaaatag aaaatgagat
    18841 cgctaagaca aatttcacta aatctgctat atctcaacgt ttcgtagcta ttaaattatt
    18901 agaaaaagat ccagaatttc atcgttttct gaaaagaaat gaatatgctt ctctgaataa
    18961 cgtagtagat aaatgtatcg aaaatattga aaaacaatat aatcgaccat cagaattttt
    19021 atttacagat gtgaggtatg gttttattaa aggtgcactt aaagagacat taagttattc
    19081 aaaagaggat gctgaccaaa gtatcgataa gctagataaa atattgacac acaagatctg
    19141 gggaatcccc attttccttg cttttcttta tgttatgttt ttttctacct tttatttagg
    19201 tagttatttg caaaattgga tggaatcagg agtagattgg ttcaaagtat ttttgtctgg
    19261 atttttgcca gcatcattat ttaaaaattt attaatagat ggtgtgatta ctggtatagg
    19321 tagcgtttta gtctttttac ctaatataat gattttattt ttatttatag cctttatgga
    19381 ggatacaggt tatttgagta gagtagcttt tttgatggat aaattaatgc atcgtatagg
    19441 acttcatggc aaatcattta taccaatgat aatggggttt gggtgcaatg tgccagctat
    19501 tatgtctgct agaatattag aaaatccgtc ggatagaaaa ataactatat tgataaatcc
    19561 atttttttct tgcaatgcta aattaactat ctatgtattt ataattaata tatttttccc
    19621 taattcttta gcatatatgc catttatctt atacttaact ggcgtattaa tagcagcaat
    19681 tgttgctatt ttatttagaa aatttttatt tgctaaagag tcttctccat ttgtaatgga
    19741 attaccgcct tatagaatgc ctacatttaa tgctattata aaacacatgt ggtttagagc
    19801 ttctatgtat ctaaaaaaaa ttggtactac tatttttata gcttctattg taatttggtt
    19861 tttatctcat ttcccattag ataatgacaa tactagattg atagagacac aaatagcaag
    19921 aatcacagaa actgaaacaa atacagcttt gttggattct ttacataccc aattagtata
    19981 tgagcgtatt tctaactcat atttagctaa aataagtatg tttttggaac ccatatttaa
    20041 acctttaggc tacgaatggc acgtaatatc tgcacttatt agtggttcgg tagctaaaga
    20101 gcttgtagta tctacattat ctatagtatt gcctaataga ggagccatgg ctaatatatt
    20161 gtcgcctcca tctgctgctg ggttattgtt ttttgctttg ttatattgtc catgtatagc
    20221 tactttatca actattaaaa aagaaacagg ttcggtttgg tgggctatgt ttactgtagt
    20281 ttatacaact ataatagctt atggagtagc tctgttagtt aaaagtattt ttacattaat
    20341 attataattg gtttaatgat atttataaaa ttttattatt gatattttat tatttatttt
    20401 gtagtttttt aaaaattatt aataaattgc ttatatgtca ttaactccgt taaataatat
    20461 tggtgaattt gcactgttag agagattagc caaattaata aatatttata acaaagataa
    20521 agtaatcaaa ggtataggtg atgatgcagc agtcattaaa ccagatatag acttagtaca
    20581 agtagtaact actgatatgt tagtagagaa tatacatttc gatctcagtt ggatgccttt
    20641 gaaacatttg ggatacaaat ctatagtagt aaatgtaagt gatgtagtag ctatgaatgc
    20701 tataccagct catgtaacag taaccctagc gatatctaat agatttactc tggaggctat
    20761 agatgaatta tatgaaggaa ttagattagc ttgcgaaaat tacaaaatag atataatagg
    20821 cggagatatg gtgtcatcta atcaaggact tataatctct attactgcaa tgggttatat
    20881 aaataaagaa aatcttgtat atcgtaatgg agccaaacct aatgatttga tttgtgttac
    20941 cggtgattta ggtactgctt atgccgggtt acttatattg cagagagaga aaaaaacttt
    21001 tatggctaat ccttattatc aacccgattt acaaaattat gaatatgtga tagagaaaca
    21061 aataaaacca gaacctcgca tagatattat taataaacta cgagaattaa atatactgcc
    21121 cacagctatg atagatatca gtgatggtct atccagtgag ctacatcata tatgcaggca
    21181 gagtaatact ggctgcagaa tatacgaaga tcatatccca atagatatga tggtatctaa
    21241 tatagctgat gagttttata tttctcccat tactatggca ttgcatggtg gagaagacta
    21301 tgaattgtta tttacaattc cactaaatca atatgatctc ataaaaaaca tagatgatat
    21361 ttcagtaatc ggccatatca cagatgcaaa cgaaggcatg tatctaataa ataatatcgg
    21421 cgaagctata gaactgaaag cacagggatg ggatagtttc aaaaaagagg aataataaaa
    21481 taaaaaaaaa atattgtttt aaataattat atttttttgt aaatttgtaa aaataatata
    21541 atatgtcagg tcatagtaag tggtcaacaa taaaaaggaa aaaagaagca aatgatgcta
    21601 aacgctctaa aatgtttact aaaatcatta aagatataac aatagctgtc agagaaggtg
    21661 gccccgaccc agattctaat cctaggctca gattagccat ttctaatgct agaggggtaa
    21721 acatgcctaa ggataatata caaagggcta taaataaagc tatcgaaaaa gattctgcta
    21781 gttatgtaga gcttgtctat gaaggatatg ctcctcatgg tgtggctata atagtagaat
    21841 gtgctactga taatcaacaa agaactatat ctaatattag aagttatttc aataaacatg
    21901 ggggcaatct tactacaacg ggatcactaa attttatatt tgatcaaaaa ggtgtcttta
    21961 gcatcaaacg tgatcaaata aaaaatttcg atgattttat gcttgaaatt atcgatgctg
    22021 gagctgatga tataatagaa gaagatgatt ttgtaatcat atattcagct cgtgaaaatt
    22081 ttggtaacat tcaaaaagca cttgaaagat tatctataga agcagaatca tctaaattag
    22141 aacgtatacc aaataattat attaaattag aaaaagaaca agctttaaaa atactaaaat
    22201 taatagattt actcgaagat gacgatgatg tgcaagaggt ataccacaat ttagaattat
    22261 cagatgaact tatagaagca ttagaataaa caattaattt acttttatag aaacatagga
    22321 tttatttcca cgtgtctttt tgaattctac tataccatct ttcaaagcat acaaagtatg
    22381 atctctgccg atgcctacat tttcacctgg ataatgttta gtaccacgtt gacgaactat
    22441 aatattacca gcttttacat actggccacc aaatttttta acccctaatc gtttactttc
    22501 ggattctcta ccattgcgag aacttcctac acctttttta tgtgccatat ataatttcct
    22561 ttttttatgc tttaatattt aaaacttcaa ctttagttaa ataaggacga aaaccttttt
    22621 tcactctgta acccttacga cgtttctttt taaaaactgt tacttttggt ccttttaaat
    22681 gctctaaaac tttaatagta actttagcac cttctactat tggtttgcct actttcacac
    22741 cttcatcatt tgctattaag taaactttgt ctacttctaa aatgctttct agttcagcat
    22801 ttaatctgtc gatatatagc tttttgcctt cagttatttt aaactgttta ccccctaatt
    22861 ctactattgc aaacattata ttgaattttg aattgcaaaa ttattaattt tttttaaata
    22921 aaaaaaattt taatttgtat tttaataaaa aatatagagt aatctttttt gaatttttat
    22981 attatttttt tttaaacatg taatatactt tcatatctca taaattttaa tttattaaaa
    23041 agtaaagaat atttgtgatt aaaataaaaa ctagtattta ctatctatga tatatctgca
    23101 aagaaatatt ctgtctttct gaaataatat aagccaacta ttagacagac aaatgatatt
    23161 ataatactta tcgataatcc gaattgattt atttgtgtac caaaaaggcc atacctaaat
    23221 aattcgatga caccagtcat tgggtttaag taataagcag atttcaacca tggtatagct
    23281 atttgattat tagaataaaa tactggagaa ataaaaaata aaagttggat aataaaagga
    23341 attacatact tgacatctct atatttcaca tttaatgcac caaaaaatga acctattcct
    23401 aatactaata tagaaactaa taatacacaa ataggcaaaa tgtatataat atcccaattg
    23461 gggtaaactt taaaatacac catcactgga atataaatca ataatgtaat gaaaaaatca
    23521 aaaagattga ccaatagaga actcattggg aaaaaaattc taggaaaata tatttttttt
    23581 agaatattag cattgcttac aacactatta gaagtagttg ttactgcatt accgaaaaga
    23641 ttccatagca tcacccctga gagtacaaat aatggatatg gtatattgac atcgcctaat
    23701 gtaacattaa tagctaaatt aaaaatcaca aatacagcag tcatagctat gggctggata
    23761 aaagcccata aaaagcctaa aactgcttgt ttatatttta ttttgatatc tttccatgct
    23821 aaaaaataaa gagtgtcttt ataaaaagca agctctttaa catcaaaaaa actccattta
    23881 ccaggtggct ttatctctat ctccatctaa ataataatat atttgcaaaa ttaaataaaa
    23941 ttgcaactat tgcaaatata ttatttttct tattttaaag tttaattttt aattttgaaa
    24001 aaattaaaaa aatgttaaat aaaaaaatta ttaagtctat ttttattttc tttttaccat
    24061 tgtatttgtt tgcccaatat gattattata tatatgatat agaaaatacg tttgacggag
    24121 tttatgatga gagatttgct accatattat tattagatac ttcgtgggaa ttcaaatttc
    24181 ctgtgataga tttagctaaa ggcaatgcat tgcaattatc atttgatatg ttagtagatg
    24241 aagtatatga tctttatgta gtttttagtc cttgctcgcc agaatggcaa atcttagatg
    24301 agcctatcac tgattttatt gaaggtttta atgaaaatcc tatatttgat tatagttttt
    24361 ctcgcaatac actaatatca tatataaatt attattatta ttttcctaat gaagatatca
    24421 aatttaaaag gagtggaaat tatttactaa cagtttatta ccatgacaaa gaaaataata
    24481 aatatagtaa agcttttagt aaaagatttg tcgtaagtga gcaagcactt ttagtaaatg
    24541 ctcatgtaat atttgctagt gatgtaactg aaagatttta tcgtcaacaa gtgctcttta
    24601 gctttaccag caataatata gtattcaata atatttatga acgcttatat gtgtgtttac
    24661 tccaaaacgg tagatggata gatgctcatt gcaatataaa acctacttat atgaaagata
    24721 atattgttta tttcgatgaa tctgcaaaaa attttacaac ttttgatggg aataacgaat
    24781 ttagatattt tgatattaga agcatatcat accaaactgg caatttagcc ggaatcatgc
    24841 acgacagcga gactgtacat gtatatttgg tgccagatat atcacggcga tatactcaat
    24901 atcttagaca accagattta aatggtagat tttttatttc taatgattta tacagtaata
    24961 taaatacaga aagtgaatac tattatgtgc attttgctat caaaagtgaa cctattactg
    25021 agggagccgt ctatctaata ggagatttta ctaattatga aattattcca agatatagaa
    25081 tgcaatataa ctatgaagct aaacaatacg aactttgttt attactaaag caaggttatt
    25141 ataattatgc ttatgcatta gtgccaaatc aaacagatat attaaatatt tcatatttcg
    25201 aaggcaatca ttctgaaaca gaaaatgagt atcaaggctt gatttattat agagaagaga
    25261 gtgatagata ttttagactt ttacatgttt ttagtatttc gaaatgaatt gtaattattt
    25321 aaaaaatatt ttaataataa tatttggcat atattttgag caaaaataaa aaattaaagt
    25381 aattatgaat aaagtcagaa ctgcattttt attaggatta atgtttgcac tatttatggg
    25441 tgtaggatat ctaataggtg gtgagagtgg aatgtttata gcatttttta tcgctttatt
    25501 gatgaatggg atctcctttt tttattctga caaaatagca ctatcgatgt ttagagctaa
    25561 agaagtttca gaatccgaag cacctcaact tcatagaata gtggaagaat tggctactga
    25621 ggctggcatc ccaaagccta gagtatatat ttcaaacata gaagtaccca atgcttttgc
    25681 tacaggtcgt agtcctaaac atagtgctgt cgcagtaacc aaaggattat tagaaatatt
    25741 agatcaaaat gagataaaag gagtattagc tcatgaaatt gctcatattg ctaatagaga
    25801 tatcctgatt tcttcattag cagcagtgat agctagtgct atagctatgt tagctcgtgt
    25861 tgcttatttt gcaaatatct ttggtggtga taataaaggt ggtggaatcg gcaatttatt
    25921 agctactctc attatgataa taattactcc tattttagct atgataattc aattagctat
    25981 ttcacgtact cgtgaatatg tagctgatga aaaaggtgct aagcttgctc gtaatccttt
    26041 atatctagct aatgctttac gaaaaatgga tgcttatgct aaaatgaaac ctgttacaga
    26101 aaatgaaatt aatcctgcag ctactcatat gctcattatt aatccattca aaaaaggagg
    26161 attagcagct ttattatcaa cccaccctcc gattgaaaaa agaattgaga aattagaagc
    26221 tatggcgtat tgaaagcaaa ctaattttta ttaaaaaaat attttattac aatcattgtt
    26281 aattttctca tttttattaa ttttgcattt aatttattta tcttttaata attatgaata
    26341 atgctatagt agtttctaat ataactaagc gatatagaga acatacagct ttagataatg
    26401 ttagctttga agtacctacg ggaagtattt ttggtatttt aggtccaaat ggtgctggca
    26461 aaacaacact tttgagaata atcaataata tcataccacc tgacaatgga gaaatattac
    26521 ttttcgatga gccactgaca gataagcata tggagcagat tggttatcta ccagaggagc
    26581 gaggcttgta taaaaaaatg tccgttggcg atcaggtact atatctttca cagctaaaag
    26641 gattatctca gaagcaagcc agagaaagga ttaattattg gttcgaaaag cttgaaataa
    26701 aatcttggct tggacgcaaa gtagaggaat tatctaaagg gatgcaacaa aaagtacaat
    26761 ttatcattac tgtgattcat tctccgcgac ttatgatctt tgatgaacct tttagtggtt
    26821 tcgatcctat taatgtagat atattaaaaa atgaaatcat agaattaaaa aataaaggag
    26881 ctaccatttt actctctact cataatatgg agtctgtaga agagctttgc gatgagatag
    26941 tattgataga taggtctaag cttatactct acggagaagt agatgaaata aaacggaaat
    27001 ttagtaaaaa tcattttgaa gtcacttttg ctcacccaat agatgataaa aaaatagaaa
    27061 actggcaaaa aaatcatcct cattttacta tcaatagagt taataaaaat aataatgaca
    27121 aaaacactat atctatcaca tctaacttgc caataaaatc taatgaaata ttactattag
    27181 taatagaact aggagagatc atctctttta gagaacagct acctagtatg catgatatat
    27241 ttgtaactgt agtacaacaa aaagaagaaa tctacgatca tgaagagcaa attacctata
    27301 ataattaaaa gagaatatct aaatatagta aagaaaacta gctttattgt aattacatta
    27361 cttgggccca taatattagc tagcatattt gttataccta tatatatttc tcacattaat
    27421 gaagctcaaa tatcagtggg tgtgatagat gaaacaggtt ttatatacaa ggggctcgag
    27481 actaatgctc tagctagata cactttgtta tatctgcctg tagacagtgc taaaaagtta
    27541 ttagatgaag acaaatatga tgctatactt tatattccta tctcatttat taactcacca
    27601 cagacaccac gtatatggtc taaaaatgct ataaatcaat ctgtaataaa taatatagaa
    27661 tcagtactta aaagtgaatt agaaaaacat agattagcct tatatggtat caataatcaa
    27721 atcctcgagc aaataaatgc agacgtaaaa gtaaataatt atacttataa agggggagta
    27781 gaaaaagtag cttctagtac tgtagctaca attttgggct ttgtaatggc attaataata
    27841 tatttctttg tatttatgta tggctctatg gtattgcgtt cagtaatgga agagaagctt
    27901 aatagaattg tagagatcat tatcagctct gtgaagtcta ttcatctaat gatcggcaaa
    27961 gtaatagcag ttttgttagt aggtcttaca caatttgtta cgtggatctt gctaacagtg
    28021 ttaatcattt tcggagtaca gcaagctttt cctcaacttt ttaaatatac cgagccagaa
    28081 atcgtaaata tacaaactaa aggacttaca caatcagagg ctcaacaaat agcaaatgct
    28141 gccaaattac aagaaagtcc ttctaataat gcacttttag ctttagctaa tataaatatt
    28201 ccattatatc ttttattgtt tttattttat tttatctttg gttatctgat ttatgcaacg
    28261 ctttttgctg ctataggtgc agcagtagat aatcaagatg atactaatca atttatgcta
    28321 ccgatcacaa taccactaat cttagctatc atttgtttgc aacctatatt taacaaccct
    28381 acgggcggag tttctatctt tttcagcctt tttcctttta cttctcctgt gataatgatt
    28441 ggactcatac cttttaatcc accattatgg cttattataa catctatggc tatcttagtg
    28501 cttactttta tactatcagc atatatttcc tctaaaatat atagaattgg catacttacc
    28561 tatggcaaaa aaccttcata taaagaactg tggaattggc tcaagaaata ttaatttttt
    28621 tatgattagt aattgcatat aatcattaat atacaatttt tttgttttaa actttgattt
    28681 tctggattta aggattagtt aagaatgtat cttattttgc aaagctaaag ttcactatca
    28741 tttaattgtt taataagtat tcccgttttt atagatttat acgacaacat agatattacc
    28801 ggtagattat ttttatattt gaaaaaatga tttaccaaaa tacaataaaa ttatttttat
    28861 atatttttgc acaataaaaa aaaggaaata ttgtggctaa acaaagtaaa gaagaaaaaa
    28921 aagaaaaaaa atctagtaca agtaaaaaaa caacaaataa aagtagtacc tatacattaa
    28981 aaggtaaaaa tttagtaata gtagagtctc cggctaaagc caaaactatc gaaaaatatt
    29041 taggaagcga ttttacagta ttatcaagtt ttgggcatat acgcgatttg ccaaaaggtg
    29101 agttaggtgt agatgtagag aaaaatttcg aacctaaata tgtgattata aaaggcaaag
    29161 agaaagttat aaatcaatta agtgctgcta gcaatcaaag tgaattggta tggttggcta
    29221 cagatgaaga tagagaagga gaggctatta gctggcatct agccgaagca ttagaaatac
    29281 ctgaagataa attaaaaagg attacttttc atgaaattac aaaatctgct atcgaagagg
    29341 ctattaaaaa tccacgaggt atagattata atcttgtaaa tgctcaacag gctcgtcgta
    29401 ttttagatcg tctagttggt tatgaattat cacctgtttt atggagaaaa atagctaaaa
    29461 atctttctgc tggtagagtg cagtctgtcg gattgcgtct gatagtcgaa agggatagag
    29521 aaattgaaag attcgttgcc gaagaatcat ataaaatttc tggaaatttt ttcttattag
    29581 aaaaaccaga tattttactc tcagcagagt taaatcatga atttaaaaaa gaaaaagatg
    29641 ctttaaattt tttagaaaaa tctaaaaatg caatatttac tatcaaagat ataaagcagc
    29701 atcctgctaa gcgtactcca ccaccgccat ttatcacttc tactctacaa caggaggcag
    29761 ctaaacagct aagatttagc gtttcacaaa caatgaacat cgctcagcaa ttatatgaag
    29821 ctggattgat cacatatatg agaacagata gtgtgactct tagtaattta gctctgaatg
    29881 ctgctaaaga gtttattact aaaaattatg gcgaaaaata ttataaacgt aggcaataca
    29941 aaaataaagt aaagggtgct caagaagctc acgaagctat acgacctaca gatttatcta
    30001 aatctactat agaggccaca caacaaatga aaagactcta tgagcttatc tggcgacgca
    30061 ccatagcttc acaaatgtct gatgcagaat ttataagagc taccatttct attgcatctg
    30121 accagaataa acatctcttc ttagctaaaa gcgaatcttt agtttttgat ggttatttaa
    30181 aaatttatca agaaaaagat gatgatgatg atgtaaagga aatcacctct gcaatagata
    30241 tttctaaatt taaaattgat gatagattgg actttaaaga tataactgcc aagcaatcat
    30301 ttaccaatcc tccttatcat tacaatgaag ctgcactagt taaaaaatta gaagagttag
    30361 gcataggtcg tccatctact tacgctacta tcatcactac tattcagcaa agagattatg
    30421 tagataaaag gagcatccct gctaaggaga gaaatataaa aatttatact ttagataatt
    30481 ctaaaaaaat aaaagataca gaaaagaaag aaaaatatgg tggtgaaaag caaaaattaa
    30541 tctctacaga gcttggtaag gtagttacag attttttaat gaaacatttc cctgatatca
    30601 tggattataa tttcactgct gaaatagaag aacgctttga taaaattgcc ctcggtcaag
    30661 aagaatggca agatatgctt agagattttt acaaagattt tcatcccaaa gtattagaag
    30721 ctatagaaac ttctagcaaa caacatattt ctcgattatt aggagtggat cctaaaacta
    30781 ataaaaatgt ttacattact tatactaagc atggtatggc attgcaacta ggcgattata
    30841 ctgataacga aaaaccttca tatgtttcta taaataagag acaaaatatt gatttcaata
    30901 aaataacgtt ggaagaagct ttgaagtttt ttgaactacc taaagagctc ggcgattatc
    30961 aaggtagtaa agtggtagta aacatcggta gatatggtcc gtatgttttg tataaggata
    31021 aattttattc tatcaaaaaa ctagataaac ctttgacaga gtttacactc gatgaagcca
    31081 tcgaaataat caaacaacct actggactta ttaaaactat ttctgaaaaa ccattgatac
    31141 aattattgat aggtcgttat ggaccttata ttaaaactga tgatggcaat atacctatac
    31201 ctaaagggac agatataaat actatcaccg atgatgaaat aaaatttatc atagaaaatc
    31261 ataaaaccaa aaaaaataaa aaatgaaaga gcttcaatat cttttcgaac cttgctttta
    31321 tgattttagt tttaataatc ataaatcaca tttatattct aatgttatca ctatagataa
    31381 actagatgat aagtctatgt ctgatattaa ttctttagca attattaatt tctcaataaa
    31441 tactgggaaa aaaaacttta ttaatagctt atataagctt agatcttacc tttattcgta
    31501 tagaaaaata gacaatgttc aaataataga tctaggaaca tttataaaca atacagatgc
    31561 tttcgaaaat attaatggtt tggcttatgt attatctgat ttattattaa aaggtgcttt
    31621 gcctataata ttgagtgatg atagatctgt agtattagcc attcataaag cttatgagtt
    31681 aataaaaaga ttttataatt tggcttttat tttgcctttt atagatgtga atgcagacaa
    31741 tctgtgggaa caatctaaag atcttttgcc gtggcataat gaaatattta aagataacaa
    31801 tctactaaat aatgctactt tcataggcta tcagagttat ctgactgata atgaagcact
    31861 agaatgggta gaaaataatt tttatgatac actcaggctc ggtgacatac gcgaaaatat
    31921 taaagctaca gaaccatata ttagaaattc ggatatcgta tgcttcgatt tgaatgctat
    31981 gaaaaaagct gaagccccag ccactatgct cactctacca aatggattat atgcagaaga
    32041 actttgccaa ttagcttttt atgctggtat gtctgacaaa tcatctgttt ttacttttta
    32101 taattattat cctgataaag atttgcaaga tcagaccatt caacttttag cgcaaactat
    32161 atggtatatc gtagaaggcg tttcgcttag ggtaaaagat tatcctttta ctgctaaaac
    32221 tcaaatgata aaatatactg ttattatgga ttcggctgta aaggaattga tattttatag
    32281 aagtcctcgt tcagatcgtt ggtggataga actacctatt tacaacactt cgcaaattca
    32341 acggcatact ctcatcccct gcttgtatga agattattta gctgcattac aaaataaaat
    32401 tcctcatata ttttggaatg ctattaaaaa atctaaaaaa taattaattt cagtattttc
    32461 tgaaattaat tatacaaaaa atacaagtaa aactgaagaa aaaaagattt atgatttgta
    32521 ttttagaatt tatttgttat tgatttttat atataattgt ttttaaaaaa aaagcctaaa
    32581 tcaaaaaatt ttatttaaaa taatgattta aaaaattata aagctgttgg cctctaagat
    32641 tttttgcaat gatgttataa ttttcgtcta ataaaaaatt gtaaggcacc acacttactc
    32701 cataaagttt tgctgcttct gaattccaat attttagatc acttacgtga tgccatgtga
    32761 gtttgtctgt attaatggca ttgatccacg attgtttatc tttatctaag gaaatggcaa
    32821 aaacttcaaa ccctttagga tgaaaagttt gatatgcctt tactactaaa ggattttctt
    32881 ttctacatgg gccacaccag ctagcccaaa agtctactaa aattaatttc ccttttatag
    32941 aatataattc taatgtatct ccattgggag atggtaattt aatatttggt gctttagagc
    33001 ctatacttgt cctaagtaat gcttgggttt tattatgaaa atttaaaaca ccagggtgat
    33061 ggggataatt atgtagcaaa gcactgtcta caattttata tgtttcagca tagccatcat
    33121 ctatatttaa tcgttcaata aaaaataaat ttgctaaact acttggattg cttctaacta
    33181 tattatttat gtaagtattt ttgacattat gcaaattgat aaattcctgt tctattattg
    33241 cttttgtatc tattgatttt gtttgaatat aaagcctttg taagctatct aattgttttt
    33301 gcagattagc aagtatccat tctacttcgt aaaccataat actcgaagga gaacctttta
    33361 ttattggttt atttcctaat tgatatgggt ttagtaataa atgtactttc tcgccaggtt
    33421 ttaaaactaa ggccaaaaaa tttgttctaa tgtcgaaata caatttataa atctcttctg
    33481 cagatgtctg ctctaatgat atataaaacg aatctgagtt gataataggc acttttatgg
    33541 tgagtgctgc aggatttaga taatttgata aggtgatttc agaatattga gttggagaaa
    33601 taaatttacc aaatatttct attttttgtt gacttaatac tattgacggc agcgctgtca
    33661 atagtataat aagaagtttt atatttttcc ccattttttt atttcaaaat tatataaaga
    33721 atttcaaacc attcattttt ttattactta actatgtgag taagattttc aggataacag
    33781 acatgtgtca aatataaacc ttgtggtgga gcaattaatt taatcttgtt tttatctttt
    33841 ttaatcagta aatcattaaa tgttttttca tctatatcac ctaaacctat tgaggtcaat
    33901 accccgacaa tgcttcttac catctgtcta agaaatctat tagctgtaat tgaaaaaatt
    33961 ataaaatctt tatgttcttt cacaattatt tcgtggatat tgcaattata atttgatata
    34021 ggcatttttt tagcaaaagc actgaaatct ttgataccga ttagagatgg taataatttt
    34081 tttatattat ctatattcaa aggtttagac atttgccagg ctctattaat aaaaaaagga
    34141 tttttatttt taataatcaa ataatgatat gtacgactaa tagccgaata tctagcatga
    34201 aaatcatctt gcacactatt tatattttta atagcaatct cagttggtaa aatagcattt
    34261 agattatata atatcctatt aggattttct atattaatat ttatgtcaaa atgagcaaaa
    34321 aaatttatag catgtacgcc tgtatcagtt ctgccacaac caatagtagt aagatcttga
    34381 cgaaaaaata ttgtaagtgc tttatctaat tcaccttgaa tagtaggtag atttttttgc
    34441 ttttgccaac caaagtaatt agtacctaca aatgataatt caagtaaata tctcatttta
    34501 taaaaaatct aaagctacaa atagcgatta atgaatttta ttatttattt aattttttga
    34561 aagattatca ttcactatac acatagtatc acgtgcaatg acaagttctt catttgtagt
    34621 tgctactaca atataagcat tagagtctgt agtagatatc agagcatctt taccttttgc
    34681 agaattattc aaattagaat ctaatttaat accaagaaat tccaaatcag aagctatttt
    34741 ttctctcata aagctactat tttcacctat gccaccagtg aagatgagca aatctagccc
    34801 attcatagct gctgcataag caccaatata tttttttacg cgatatgcat atacatccaa
    34861 agctaattga gctcgtttat taccattttt agctgcagat tctatatcac gcatatcggg
    34921 actaagacca gagataccaa aaaccccact ttttttattt atcaaatcat tagcttgttt
    34981 agtatctata ttttcttttt ccataatata aagtaaggct cctacatcca agtctccgca
    35041 acgagttccc attatcaagc cttctacagg tgtaaacccc atactattat caattgattt
    35101 accattttct atagcagtta tagaagcacc attacctaaa tggcatgaga ttatctttaa
    35161 atcatcaata tttttaccaa tcaatttagc tgatttatca gatacatatt tatgactagt
    35221 accatggaag ccataacgac gaatttccat cttttcataa tattcatagg gtattccata
    35281 aatataagca taatctggaa tagattgatg aaaagatgta tcaaaaactg ctacttgggg
    35341 taatcctgga attaattgtt cgacagctaa aataccttta agatttgctg gattgtgtaa
    35401 aggagctaat tgtatgcatt tacttatttc tgattttgtt tcattattta tgagtacgct
    35461 atttttaaaa tgttcgcctc catgagctac cctatgacct gcagcagcta tctcattaag
    35521 agattttata acacctagtt ctttatctat caatattttt ataattattt ctatacctgc
    35581 tgtatgattt ggtataggct gctcatatat tattttttct tttcctatag gagtatgatt
    35641 tacaatgccc attggcaaac ctatcctttc tagtaaccct ttagctagta cagtttcatc
    35701 tgctagcata tcaaacaatt gatatttaat gctagaacta ccacaattaa gaactaaaat
    35761 tttcattttt ttgcaaagtt aaacaagaat tagaaaaaat aaaaatttta attaataaaa
    35821 caatgaatct gttttcattt ttatatattt atttacagaa aagagagttg ataattcagt
    35881 tatcactaca cctattataa atataaatat aaaaagaaat ataaaatgtt gttgagtaac
    35941 aactccatta agttcaggaa taagtttata gagttgattt attattactt ctaatcctag
    36001 aatagctaat atagatgcta atacacccca gaatagggca tcttttaaga aaggtttttg
    36061 aataaatcct ttcttagctc ctacaagtag cattgttctg atggtaaatc tttggctata
    36121 tattgtcaag cgtatagtca agcttagcaa tgtaactgcg attgctaaaa taataatagc
    36181 tactacaagt gttatggtac tgagggtatt tttatttttt tctatttcag ttagcacatt
    36241 agtaggataa tacacttcgt ctacaatatc tttttgtttt atctgagtta ctagattttt
    36301 tattacttca tcatttaaat aatcagcgga tatagtagcc tctaagagag atggcaaagg
    36361 aatattttct aattctaatt tatctgcaga tatatcacct aactcagctt gaagttcttc
    36421 gagcattttc attgatggga tgtaattaat agcagaaaat ttgcctgtag cttctaaatt
    36481 gtgttttagt aatgagatct ctacctcact agcagtaggt tttagcatta tttgtatagt
    36541 gaaattttct tttaatttat tctctagtgc tgtaccatgc aaatagataa tacctgctat
    36601 tcctaatagt aacaatacaa ggcttagagc taatattaat atggcatgaa tgcctctaaa
    36661 tctcataata tattatttat tgtgcaaata tataaaaatt tgagataatg atatttttgc
    36721 caataacaga tttatatatt ttcaatcatt tatataaaaa attgaatagt ctactaataa
    36781 cgagcaagtg atacctattt taatctttct cgttgttttt taaacttatt ttcttttagt
    36841 aattttacaa aaaaatatta taaatgtcaa atataacagg agaagaaaaa agaaacttag
    36901 ttattattgg ctcaggtcct gccgggctca catcaggtat ttatgctgct agagcagatt
    36961 tacatccatt attatatgaa ggattaatca gtgggggtca gttaataaat gctacattaa
    37021 tagaaaatta tcctggtttt cctgatggga tacaaggtat ggaacttatg gaaaaaataa
    37081 aaaaacaggc tattaaattt ggtgcagatt tgcgtttttc atcaatagaa aaagttgatt
    37141 ttttgcaata tccttttctt ttatgggatg acaatgatat agctatttct gccttatctg
    37201 taatcatcgc tactggatct tcgccacgac ttcttggcct tccttccgaa aaagaatatc
    37261 taggttatgg agtttctact tgtgctactt gtgatggtta tttttataaa ggaaaagtag
    37321 tagctgtagt aggtggtggt gatacagctg ctatagaagc tatttatctg gctaatctag
    37381 cttctaaagt atatattata cacagacgtg atcaattgag agcatgcact tgtaatcaaa
    37441 aaaagatttt taatacacca aatattgagg ttatctggga tagtgtggtg gaggaaataa
    37501 aaggtgaaac caaaggtttt actaaaaatg tcaatgctat agttttgaaa aatgtaaaaa
    37561 ccaacgaatt gaaaactatt aatgtagatg gtgttttcat tgctataggt catcatccta
    37621 atacagaatt atttgaagga caattggaat taacaaatga taaatatata aaaactattc
    37681 ctggaagtac taaaacgagt gtagaaggaa tctttgcttg tggtgatgtg agagatccta
    37741 tttataagca agctgttata gctgccggtt ctggagcaat ggctgccatt gatgcacaaa
    37801 aatttttgga aaattttaat ttttgatttt ttataaaata gggtgtgtat taattgtgtt
    37861 taggtaataa aattttttat cgtatctttg tacttatcaa ttaaaagtta aaatgaaaaa
    37921 agaatattta ggattagaat attatatatt tgataattta gaagagttag ataaaaaaga
    37981 taaagaatta gtaaaaaatg cgatagatgc ttctactcgt gcatatgctc cttatagcaa
    38041 atttcatgta ggggcagctg tcagattatc aaatgatatt attattcagg gaaataatca
    38101 agaaaatgca gcttatccgt caggattatg tgctgaacga gtgacacttt tctatgctca
    38161 tagtcggtat cctaatgaag taatagtaac tatagccatc tatgctaata atgaagataa
    38221 tacctataaa gacattgtaa ccccttgtgg agcttgtagg caagtgatgg ctgagataca
    38281 atctgctcaa aaaaaacctc taagagtgat tttactcagt cccactaata gtggtattat
    38341 tttgccttct attgatgtgt tgttgccttt tcagtttgaa ttataaaaat attttagata
    38401 aaaaatgaaa aaaattaaag catacttaat tttagtaata ttttttattt tatcaattat
    38461 agtttgctgc aaaaagaatg taccacaagg tcatgtaagc atctctggta gactcatcgg
    38521 tggtggaggc aagccgttgt atttcgctac tgtacatcct aaagaaatag aattattaga
    38581 tagctgcact ctcaatgagg agggtttctt taaaatcaag cttgctatag ctcaaccaca
    38641 atttattaaa gcatacagca atgatactaa ttttataata ttagcagctc atccaaaaga
    38701 agatatctac atcagtggtg attattatca attagatgct acatattcag tatttggttc
    38761 tccttctagt gaaattatac ataaatatag atcttttaca cgtaaaaatc tcgaaaaatt
    38821 gcacatgctc tccatctact gggaaaatca taaatatgat aatgataatt taaatataaa
    38881 agatagctta gatagaaaag ctttagaaat atttaaaaat cagagagatt ctgctctagc
    38941 tctgataaac aaaaatccag aagattttgc aaatattttt attatttatc aattttttat
    39001 taatcaacct ctattcgacg aatatcacga cttagaaatt tatcaatggg tattaagtaa
    39061 tttagaaaaa aattattctg acaatgaaca cgtaataaat ctaaagatga ggatcaataa
    39121 aataaaaacc aaaatagcag agcgtgatag tattttaagt agattagcta ttggtaagcc
    39181 agctccagat tttgcactgc ctttatggca gtctgattct gagtttgttt tgtcattaga
    39241 acgtggcaag tctttattac ttcatttttt tgctggtgct tctctatcaa gtgtaaaaga
    39301 tatgtctaag ctaaaaaaac ttttaccaaa actctcaaaa tataaaatta aattaattac
    39361 tatctttctt gattatgata tggagaccat cgcacgtgca attgatggag agggaataaa
    39421 ttggactatt tgttatgatc ttagtagcac acgtagtcct gttgctaaat tgtatgctgt
    39481 agagcaattg cctatgtatt atttgataga tgctaatggt attataagag ataaagcttt
    39541 tactgctgac agcataaatg tttcatctat caatactatt caatgaaaaa acaaatttct
    39601 tatattcctt attcactagc taaagaaaaa aaattatatt ttatatctga tattcatttg
    39661 gggatgccta atgagagcga tagcaaaaat agagagattt tgctcgttca atggttagaa
    39721 caagtctcat ctgatgcaca agcaattttt atcttgggtg atttatttga tttttggtat
    39781 gaatacaagt atgttgttcc taaaggatat actcgccttt tggggcaatt agctaaaatg
    39841 aaccacagtg gcatagaaat tcattttttt gtaggtaatc atgatatgtg gacttatggt
    39901 tatttagaaa atgaaatagg atttaaaata tatagtgata ttgctggatt ttatatagat
    39961 aatatacgaa ttcttttagg tcatggagat ggattagggc ctagagatta tggatacaag
    40021 gtaatgaaaa aaatttttaa gaataatgtt gctcagaaat tattttctat gctccatcct
    40081 tattttgctt ttaaaattgc tcatagatta tcatcgacta gcagaggtga ttatgaaaag
    40141 catatcgaag agtttaaagg aatagaaaag gaatttatca ttcaattttt agcttcttta
    40201 gatgaaagta ttaagcccga tattgccatt ttcggtcata gacatttaaa aacggatctt
    40261 ctaataaatg atattagata tataaattta ggtacttggc tactagaacc taactacgca
    40321 caatttgatg gtagcaatgt tttattaaaa acttttttta aataatagat aatcaaaatc
    40381 atccaaataa attgaattaa ttagataccc aataagtatg agtatgaaac tgagaaatga
    40441 tgtttctaat cctgaaataa gtttatataa aaaattttaa gtgagtattt agttttgttt
    40501 ataggcatct attctctgtg tctaattata aatattatta acgaagcatt taattaaatc
    40561 attttgttta aatttaataa atttgtttac taaataaatt ttttgtgatc aaaattgatt
    40621 atataattaa taaaaaatta aaattaagcc tgtggatact tctgataagt gttttagtat
    40681 ttactattaa tcatacattt tatccacttt atcagtcaat aaataaaaat aatttatcaa
    40741 aaaaagttga aaaacaatgg aatgaactaa aaagttttgt aaatgatact gctatcatta
    40801 aattaataat caataatgat tggagtagtc tacaacagga gttagaaaat attaataata
    40861 taaaggctgg tgtttggata tatgatgcag acagtttgat ctattggtct actaatgact
    40921 ttataattaa taatttaaca gcgcagcaat gggtagctga gagtgagagg ggtatttttt
    40981 tatgttacag ccaagagttt gaaaattata aattgatttt gagctggcaa ttacaagaaa
    41041 gttatccaat taaaaataat tatttaacaa ataagataaa taaaaaaata gacccattag
    41101 cagcttttga attaaaaata aataataaaa ataataatgt tttagaaaat ttagaattta
    41161 ttcctcggga tattcagcct actaaaactc aaaatttcat tgcattatta agttttattt
    41221 tgtttttatt tggtttgtat cttttgattt ctgcattgat aaataaatgg aaagctagta
    41281 ataaaatctt tgtcctttta attttaatat taacgattat cgggttatta tggatactta
    41341 ctaatttttt atctaccaaa tattatgttt ttaatccttc attttttgca ttatctctta
    41401 tttttaacaa tataggtaaa acatttttat tagtgtggct agctgctcta attatcaaga
    41461 ttgctatcaa gctattacct aatgattaca aaaggctttt ctgttttgta acctcaatat
    41521 ttttctctgg taatattttt atctggctat ctcgcatatt caatgcaact gaatttccct
    41581 atagctatta tgaatggata tctacaaaag tggatattat atttgtaata attttagcta
    41641 tatccctact ggtcatatgg acatggtcga tcagattgat gctaactcac aaaccaccca
    41701 aaaaaatagt ttatagtggt tttgcttttt atacaatttt tttgattttt ttattaaaaa
    41761 atcaaaattt ttatcttatt atctcgatgt tagggatctt actttggctg ataatgtacc
    41821 tattatcatc tatgaataag aagtcgttta ggcgctattt tatttatatt tttagtggta
    41881 tgatagtagc tattagtagt tttttattta tgcgagtgca tcatcagagt ttttcgaatt
    41941 atttactctt aaatgctgta aaaataagct ctgagcgaga tttgatacta gaaaatcact
    42001 taaatgaact ccaaactgaa atatctgctg atagattttt agtgaattat gctaatagct
    42061 tcgataccag tatgcaatgg ctgacacata taaaaaatca ctacttatcc tcatttcaag
    42121 atcagtatga tatcataggt ttcatctgta gtagtcatga taaattttta aataataatt
    42181 tagaaatcaa ttgctggcaa tattatgata gcatagtgga gtattcgcaa gcacagcaca
    42241 tatcagataa tttatatttc attactaatg ccaatattac ttactattta tacaaaatca
    42301 tgcttcatag tgagctacca caccaattat atttagaact ttacaaaaag aaacctttta
    42361 gaccagaagg atatttagaa ttattagtaa atatagagga agagcaatta ctccctggac
    42421 agctagatta tgctacatac tataataata cattaatgca gagctatggt aatacaattt
    42481 ttcctttaca agtagataat gaaatagatg cagctcctat tggcaaatta tttaaatatc
    42541 atgataaata cgccatcaaa agcgaaatag acaataatag atttattgtg ttagtgtcta
    42601 atcacacttt accaaattgg ttgagtatct tttcattttt tattataata attctattgc
    42661 tttatttaat ttattacata ttaataaaag gtattaacat taaaaagatt tttagaagtt
    42721 atgcgggtag attacaaatc acattgatca gtattattac tattgtcatt ttggtagcag
    42781 gtattttgtc tgtcaaatat atacaaaaca catactcacg gcaattagaa aaacaatgtt
    42841 ttgagcgcaa ttatggattg tacttatcat tacaagacga aatcgtaaaa aataatataa
    42901 aaatcaactc gaccagcata gattcactgt tatggaaata taaatactct tacttttcgg
    42961 atttacattt ttataataca gatggcactt tattatcaac gacacaattt gcattgttta
    43021 acgatcattt gttaacaaca ttgataaatc cagatgttta ttggacaaac aaatggaaaa
    43081 agtattggta ttactcaaat aaagaaaaaa taagtgagtt tgaatatcgc aatacatata
    43141 tgccagtatt tagaggtaat aatataatag gcatattgtc tatacctatg tttagccaga
    43201 tcacagaatt acggggagaa ttgagtgagc taataggtgc ttttataaat atttatgcca
    43261 ttttactctt gatttctcta atttttactt ttttaataac acaatatata aatagaccct
    43321 tgcaattaat aagtaaaagg ctaaaatcac taaatttatc taaacaaaat gaaaaaatag
    43381 aatacaaaag taatgatgag ataggacaat tagtaaagca atataatcaa atgatagatg
    43441 aattagctga aaaagctgaa aagctggctc agaacgaaag agaaatagct tggagagaaa
    43501 tggcaaaaca agtagctcat gaaataaaaa atcctcttac acctatgaag ctaagtgttc
    43561 aatttttgca aagaatatgg aagcaagata aaaaaagatt tgaacaagat atcgaaaaat
    43621 acttaacaac acttctagaa caaatagaac aattatctga aatagcaaca gcattttctc
    43681 aatttaataa aatgccagaa ccattaagca aggaagtaaa tctcataaca cttttacaaa
    43741 atttagctgc cctgtttcat agtacagatt atgatattaa tattcattta gaatctgata
    43801 aattgacagt aataggagat gagagtagac taaatagagc ttttactaat atattgcaaa
    43861 atgccgtgca agctctatca aatgaaagac gcggagagat aaacatttat gctaaatcag
    43921 ataataataa tgctataatt ataatttctg ataatggtat cggaatcgcc aatgagatgc
    43981 tagaaaatat atttgaaccc aatttcacta ctaaatcatc tggtaccgga ctaggcttag
    44041 caatagtaaa gcatataatc gaagacttaa atggtaaaat attggtagaa agtcttttag
    44101 gtgaggggac aaaatttacg attatcctgc cattgtgtta ttagatgata aaaaacatgt
    44161 tgcaattata aaataattgg ctaggctaaa aaataaatta ttatctttga taaaaaaatg
    44221 agtgaaccaa atataaaaac aaaatcactt cctaaaattt ttgtattaga tacatctgtc
    44281 atcttgtata atcatgaagc tatttatagc tttgatgaca attttattgc tattcccata
    44341 acagtattag aggaattaga taatttcaaa aaaggaaatg aattaattaa tttcgaagct
    44401 cgtgaattta taagaatctt agatcagata tcatataatc attcaattaa tgaatggata
    44461 cagtttagag aaaatggggc ctctgttaga gtcatattag atgaaggatc cgaccctgat
    44521 gctaataaaa tttttggtgg ccacaagcca gatcatagaa ttttaaatgt agctataaag
    44581 ctatcaaaag aatttcctga tcgtaaagtg attttggtgt ctaaagatat aaatcttaga
    44641 ttaaaagcaa gagctctgaa tataatggcc gaagattatg aaacaggtaa aataaaagat
    44701 ttagaaaaat tatatactgg caaaactaca ttagaaattg atgacaaatc tataatagat
    44761 ctgatgtata aacaaggctt tgtggaacca aatgaaatag gcatcactga tcctgtccct
    44821 aatcattatt ttatcttaaa atataatagc aactccgtat taggttatta taatccatta
    44881 acacaagtaa tagagaaaat tgtcaaaaaa ccagcagcac gtatttatcc acgaaatgca
    44941 gagcaggtat tttctattca ttctattgtc tcgcctaata ttaaattagt tacattgcaa
    45001 ggtgttgctg gtacaggtaa aactttatta gctttagccg gcgctttaga aactaaagat
    45061 gatttcaaac aaatatattt agctagacct atcgtaccac ttagtaataa agacattgga
    45121 tttttgccag gagatataaa aagtaaaata aatccttata tggagccact atatgataat
    45181 ctgcgtttca taaaatctca aaataataac tatgcttttc agcaaaatat agataagatg
    45241 ctcgaagaag ataatctagt aatagccccg ttagcatata ttcgaggtcg tagtatctca
    45301 catgtatgct tcatagtaga tgaagctcag aatttaacac ctcatgaggt aaaaactatc
    45361 atcactagag caggagaagg tactaaaatt atatttactg gcgatattag acaaatagat
    45421 accccttatt tagatgctcg aagtaatgga ttatcatacc tcatagatcg tctaaaagga
    45481 catcccattt atgctcatgt aaccttagaa aaaggcgaaa gaagtgaatt agcaaatata
    45541 gcaaatgaat tattatgaat ataaaaattg tagatctcgg taataagcga ttcgatgacg
    45601 cttaccagta tcaattaaaa aaagttgatg aagttttcaa taagaaaaat gaacctgatt
    45661 ttattggtta tttaatatta cttgagcacc cacatgttta tacccttgga aaacatggta
    45721 aagaagataa tttgctcatt tcacctcaat tattagaaaa gattaatgcg gaattagtaa
    45781 aaactgatag agggggtgac attacttatc atggaccagg acagattgtt ggttatccga
    45841 taatcgatct gatgaaacta aaaataggac ccaaacagta tgtttataat gtcgaagaag
    45901 tgataattaa tgtattagat aaatatggaa taaagggaga tagaatgagc ggctctatag
    45961 gtgtatggct agatcctaat acaccaaagg ctagaaaaat ctgctctatc ggtattcgaa
    46021 ttactagaaa tattactatg catggctttg catttaatgt aaatacagat ctaaaatact
    46081 tttcatatat aaatccatgt ggtattactg gcaaaggagt aacatctata gctaaagaat
    46141 tagggtatga ggtagatata aatgaagtaa aaaaatatat tttagatatt ttttctgaaa
    46201 tttttaaagt cgaaattcaa aatttataat atttttggga cttttttttt aaaaaaaata
    46261 aatatcttta atatatcaat tcatttatta atgttatata ctcattaata ctcttatgca
    46321 aattatctat tttatcatat aattgtaatt cttttgcaaa aaatgcacgt acattatctc
    46381 ctaatgtgat atttatttca ccttgaaaaa ttatttcaat aaatggtatg atattctcat
    46441 caatttcatt tgtgattatt aagcatttaa tttttttaat atccatttgc aaaactccag
    46501 catataaacc tatcggtggt actatttttt ctatttcatc agttttgatt tttatttttg
    46561 tcaatttatt gtctaatggg tttatttcag ttacaacacc gaaaaaggga aaatgagatt
    46621 gtaagtaagc attagctttt tgtatcaatc cttggcttag agcttgtctg attctagtag
    46681 atgatacagt ctctttttct atctcttgtt gtggtatctc ctctacttga aatccaaaat
    46741 attgttgtaa ttcatataaa tattcatagt caccttgttt gtcgtagccg aaatgatgat
    46801 taaaaccaac tactatgact ctagcgtgca atttatttac taaaaaattt ctaacgaatt
    46861 gctcagcggt aattttagaa aattcattag taaaatgtat tataaataga tgattcaatt
    46921 tagtctcttg taatagatgt attttttcat tttgagtgca gatcattttt aaatttttac
    46981 ctaaggtatc aggatataat actttgcgag gatgaggata aaaagttatc agtacagact
    47041 caccatttac tttttgactt aatatattta atcttttcaa tatagctctg tgtccagcat
    47101 gcacaccatc aaaagtgcca atcgttacaa caggattaga tatttcaaaa tcttcatcaa
    47161 agctataata tatatgcatt tttatttttt taaaaaaaaa tacttactca tttatttagc
    47221 atttactttt tttttaatta ttttggttct attcaataaa ttgtgtaaag taagcaactt
    47281 tttccaaaga tgttatcata tcatattctt ctagatatac gttggatggc acttttaaag
    47341 gcatagtagt gaaatttaat aatcctttaa tgcctgcctt aattaaaata tcagctactt
    47401 cgttagctac atttgttggg actgtcaata tagcaatatt tatatttaac ttttttattt
    47461 gttcttctgc ttcattgata gaataacaag gtataccaca acacatttta ttaattttat
    47521 cctggtcatt gtcaaagcaa gctattattt gcaaatattt atttttattt tttaaataat
    47581 tgagcattgc acgtcccaaa ttaccaacac ctactagagt aacatattgt actgtgttac
    47641 catctattat agaatttatc ttttctatta attgtttaac ttcataaccc cttctaagtg
    47701 taccgctatg tcctattaac atcatatccc ttcttacttg ttcgggagtt atatgaatca
    47761 gattagctaa ttcatgtgaa tagatataat ctttttgttg aaaatttagg tttatcaata
    47821 cccttctata tttacttaaa cgttctattg ttttttctgg taatatcata ttagtgaaat
    47881 ttataattaa taaaaaagac ttttaattat caataaaatt tttagtttta ttatcttcta
    47941 ttttaggtaa aataatggta aattctgtgt attcattaac tttgctctct acctctatag
    48001 ttccattata caattctatc atttttttta cgatagaaag tcccagtcca gtaccagata
    48061 aacgacgaga tttagcatct actgttctat agaattcatc aaaaatatgt ggtaaatcat
    48121 tttcattgat accaatacct gtatctttta tttttattat tatattatta tcattttctt
    48181 cgccttttat tattatttta ccgttatttt tattgtattt tattgcatta tttattaaat
    48241 tattaaataa tatttccatt tcatttttac tagcaaaaat atttaatgga ctttttatat
    48301 ctatatcgaa agttatagat ttttgaatag ctaatggtcg ttgactatca atagaaaaca
    48361 tgataatttc ttttaatgat aacaattctt tttctcctat agtgctttcg gcttgcaaat
    48421 tagtgaagtc tagaagatcc attatcagag ttctcatttc ttctaattta ttaagcattt
    48481 tgtctattaa ttccatataa ttatctatat tatcacctaa ttgcttttct tttatcatat
    48541 aaatatatcc ttctagggta gttatggggg ttttgagctc atgcgacaaa atagaaagaa
    48601 atttgaagcg tatttttcta gcttctttat ccatatcttt tattatacta tttagatata
    48661 tttgtttgct agctatctcg atagcatttt ttagttcctg tggagtaaaa ggtttgggaa
    48721 tgaaatcaaa agctccatgt ttagtagctt ctaatgctat atctaatgag gcaaaggaag
    48781 taatgattag tattttagta tcaggataat gctcatttag ataaggcata atatcgatac
    48841 cttgcattcc tggcaattta ttatctaata atataatatc catgtgagta tcagacaaaa
    48901 tttctaaagc cctctcagca ctctctgctt catatagcga taatttgata ggttcatcta
    48961 aaaatggaaa atctatccga tgtttttcta atgctctttt tattgccatt ctaatgcctt
    49021 cttcgtcatc tactattaaa atattgtata tcataacctt tattttttat ttgttaataa
    49081 ttgttggatt attttattta atacctcagg cctgatacct ttttcacaat atttatctat
    49141 aggcattaat tctggcgaat tatcaaattc attagaaagt gaataccctg tttcattagt
    49201 gatagcagta agtaatataa taggtatgtc tggatagttt gatttgattt ttcgagctaa
    49261 ttgtagtccg ctatcttcct cttccatcat caaatctatg atagcaagat caaattttaa
    49321 atctttcaga cgactttcag ctgatctttg cgaaggtaaa tatattgtat tataaccaaa
    49381 tttatttaga taaaataaca tttgatcaga aaaatcggga tcatcatcta ctaataaaat
    49441 ttttattttt tccataattt ttattttttt tttaaaacta cttaatgata tcatattttt
    49501 ggctttaata atattttatt ttttatataa aattattaat ttttactttg aggtataaag
    49561 atataaaatt tagtatatgt aggcccttta gacggatcag cattgctttc cacttctatt
    49621 ttgcccctat gcattttaat gattccataa acggtagcca aacccaaccc cgtccctttg
    49681 cccacgggtt tagttgtaaa aaatggttca aaaatttttg ataaatcact atcgggtacc
    49741 ccaacaccat tatcatatac tgagattgcc aaatagttat ctttagtctg agcttctaat
    49801 atgatttcac cttttttatt gccaatagca tctattgaat tttgaataag attagtaatg
    49861 gcttgtagca tttgttctat gtctatatat ccttcgagat ctggcggatc aactttggtt
    49921 tttactgtaa tatgttcagg tattataaca gaattaatag ctttatcaaa tagattttgt
    49981 atatttattt tttcataaac aacttgattt tttctagcaa aattaagtaa tcctttaaca
    50041 atttgcctac atctctcggc ttgcgagact atcatttcta gatcttttct tttagggtca
    50101 tcgggcggac attcttcata aagaatattt gcgtacatta ttataacacc gaggggatta
    50161 ttgagttcat gagctatgcc agcacttaat tgtcccatag ttgctaattt ttcagttttt
    50221 ttcagagctt cctgggcatc tcgcaattct ttgtttgaga tatttaattc ttctaatagg
    50281 ctatgcaatc tgtctatagt atatggaagg cacatctcgg tttcagctat accttttatt
    50341 atcgctatag catgctctct acaagtattg tatccacaag caccacaatt gagttcatct
    50401 tcgggagtag atttacctaa ttcaattaaa acagatctga tttcctcttc acttggtatt
    50461 aaatcttcaa taggggaggg ataatattct acagataaat caagtgtttc catccaatag
    50521 tttaaattat tttcccattc ttcccaatca atatttttta attttctttt aacataatta
    50581 gtaacgaaag cctccttagc aaatctatta ctcaaagaag tagttcctgg ccccattatg
    50641 cagccatcac aacacaaaag ttcaaggttt tctttgcccc aagtttttga ataaaattct
    50701 ttcagagctt cttgaaaatc ttctctgcct tcagcagcta ttatattact tttatagagt
    50761 ttatcatcaa tgtctacagc ttgtatcagc cctctagata caggaaatat ggctcctaaa
    50821 cctccataag gagggtcgaa agttgaaggt tctatatttt ctatttcaat attatgtttt
    50881 tgaattaaac ttctaagttc acgataagta attttgaaat ccacctcttc ccaatcatct
    50941 tttttggcga tacaaggtcc tataaaaata gttttaatat tatagtcaaa catatttttt
    51001 actactctat tcattgcatt cataggcgag catacaggta ataaattttc tgtcagttca
    51061 ggataatatt ttttaacaaa tttcactatt gcagggcaat ctgaagtgat atattgcata
    51121 tctggattag cttttattag ttttttaaat gtatgagcta ctaagtcggc accgaaagcc
    51181 acttcgtaaa tatagtcaaa accaagtttt cttaataaac ctacaaaatt attatgagtt
    51241 ttgaaatcag aaaattcgga tgggaagctt ggcgctacta atgcagctac tttttcattt
    51301 gtttgtaaaa gctcatcgac ctcatcagag tgctttacaa aatctttggc gccttgacta
    51361 caaactttta tgcagttgcc acaggtaatg cacctatctg gtaaaatatc agcttgcccg
    51421 ttaaatatcc ttatagcttt agctggacat tctctaacac aagtataaca aaccttgcat
    51481 ctattttgta ctgtaaaaac taattgatga tattctaatt ttcttgtttc cataatttct
    51541 cttgaattat taaatcttca ttaatattat aaatttgggt gagcaaggga tttcttgagg
    51601 ataaactttt atttgtagtt ttaaatattt cgaaagtttt gcctccacca ttaatgcatc
    51661 cattagcaca gctccagcat cctattaatg ctaaatttgt ttttaaaaat tcatgatcaa
    51721 aaaaatttgc aatctcactt attgtattaa tgcttattaa agaataattt ttatttttta
    51781 atctaaaatt atacttttta ttttcatttt ttcctgttaa aaaactatta atttttgagg
    51841 gtttatctat ttgtatattg gtttcatcta aaattaattt taataaatta tacattgata
    51901 tatcagcata cagtccgccg atagaatatg aatagttttt tttagctttg atgttaggaa
    51961 tcaaattttt taattgttct aatgtatagc catttcgcaa taaatatttt aaaaaatctc
    52021 ttacattagc aacataaatt ttgtgctcag tattactata attttgaaaa atatcatttt
    52081 tgtaagccga acaattagtg agtatcagga taggattact tttattagtc aatctaattg
    52141 cagcctgcat tggggaagct aaaggactga taaattcaat taattctgga tatttttgtt
    52201 taatatattc tatactgaca ggacataaat tagaaatgat agggtgtttt ctatctttta
    52261 aaagttgaaa atctaaatat aatttagcat ctattaattc tcctaaatta attaattttt
    52321 taaaacctaa gaattgacat aaataattaa atgataaaat aggatttttt gtaaaattac
    52381 tattaatctg agacatcaag ttgtaaacag cagcagagtc aaatattaag gtagtatttt
    52441 gtggatctat ctccacatta tcaatgtatg atttttcata taaagcacta gtaggacaat
    52501 gataagagca ttgtccacag tagtaacaat tagataaatt tataggttta tccaaagaag
    52561 aaataatagt ttttttagta ccaataaaag catgattaaa tacattgcaa tgcatcattt
    52621 cattacaaat tttaacacaa ataccacaag agatgcattt atttatattt ttagctatca
    52681 caggactaga tatatctttt tccttggaat cggtaatcac atgtctccaa ataatttcgt
    52741 ttacaccata ttgatatgca taatttaata aactgcaatt aatagcctta ggacatgtat
    52801 tacattctaa tgaatgttgt gacaagatta attctaatat attcttacga atatttatta
    52861 gcttcggagt atgagtgtaa acctgcagtg gtgtgtttac atcaacagta caagcttgaa
    52921 taggattatt aaatccatca atttctacca tgcacaatct gcaagcataa tttggggagt
    52981 aattttctaa atagcaaaga tgtggaatat catagcctaa agaaagtaaa gcatttagta
    53041 gattttcccc tagatctact ttgtattttt gattatttac tgtaatatta attttcattt
    53101 ttttaaattt atagctgaaa acttacaaac tttttcacat aaaccgcagc ctacacattt
    53161 accatctaaa ataacaaata gatttattcg tttttctatt atagcattta cagggcattc
    53221 tactatacaa agtccacatc cagtacatgc atcttcatct ataatataat ttctcaatcc
    53281 tacacattga taagaacgac aatatttatc aaatatatgc tcttcgattt catctttgaa
    53341 aaaattatat gctgatatga atgtttgcga aagacttttg cctaatgcac atagtgaaga
    53401 ttcagtaaca gtacgatcta atttattaaa tgataaaata gtttttaatc tatataaagg
    53461 aaaatgggga atttggggat tattagctaa tgatagataa ctaatcagct cagaccatac
    53521 cctagatcca tataagcatg gtatacattt accacaactt tgctctgtaa gtatttctga
    53581 tatatgttgt aaaagtttaa cgatacattg ctcttctgaa aaagtaaaaa tagtagatgt
    53641 acctaaataa ttagtttgtt tttctaaatc ataaagagat ttatccaagt tatctagatc
    53701 aaaaaatctc ccagtaactc ctccaatgtg aaagcctttt attttatttg aattattaag
    53761 tccaccacct attttgttta ttacctcatc tatactgata tccatagata tttcgtaaac
    53821 gccataatta tttatttctc cacatagagt tactacttta gtcccttgct tcgaaatggg
    53881 ggcgtagatt tcaagagatt tatcagtttt tattatttga gaaatatttg ctatagtctc
    53941 tacgttatga actaaagtgg gctgtcccca caaacctaaa ctagtgggat atggcggttt
    54001 attagtagga aaaggcattt gagattctaa taaatgtata agggcagtct cttcacctgt
    54061 aattaacata ccaggtgtca acattacatc aatagataaa gaattaccag tatttaaaat
    54121 atcatcacct aatagaccta aatctttggc ttggctgatc aatttactaa aagcttcata
    54181 aacatctaga tagtcttgat gaatagctat tatggcgtta ttaatatgca aagcatagca
    54241 agtggttatt ataccttcta gaatcattgt aggataatat cgaagtaaaa aataattact
    54301 tagactacta ggtccgaatt cgttagcatt tattattaaa tatttttttt ctgctaattg
    54361 ctcatctata gcttgccact tgatttctat aggtagagta cctgaacctc taccgataag
    54421 cttactctta aaaattaaat cttttacttc tacactactt tttttattat atagagagac
    54481 gatgaaatca atatttgtat tgtctaaaat tgtattgtct attatccaat catctccaga
    54541 aaaagaattt gtcaaaaagg gcactatctt gtcataccaa ggcatttgag ttattatagg
    54601 gatatggggc cagctttctc cataatcatc taattgtatc caaggtttca aactagttat
    54661 atcaagattt acaacacatt ctattatcca tggtattaac tcaggttcta aatttctgaa
    54721 acataaagta cgcttgagtg gatgttttat ccatactaag ggttcggcat acaaaaatcc
    54781 ttttaatcca gtattaggaa aagtttctat tataatagca tcatcattag gcgataaatc
    54841 tgtactatga ttttttaaaa taaaatgttt atcttgagag agcagagaca ataactcttt
    54901 ttcccatata ttttgcaatt ctaatgaggt gatagcatag cctaactgaa gctttatagt
    54961 gctcattttt ttataatttt ttctttaata ttattcaaga ttttataaaa agctgaattt
    55021 atatttaatt ctatttgatc tgtatggcat ttgccattac atttatgaaa ttttataatt
    55081 ggtttttctg actcatttct aaattggtaa ttaacttcat ttaaaaaatt tttaccatat
    55141 tttaggtagc aattccaccc tctacacata actatgtcat aattgatatc ggatattaat
    55201 aaatcaggga agcaagtaac cacaaaagca taaatctccg atgcaggctg tttaaaataa
    55261 atttctaatt catcaaaatg ggatttagag agtgcatatg gctctaacat tattttagca
    55321 caaaaatcta tgagagaatt aggatatacg tgccatactt ccttaaataa ttcatttatg
    55381 gtaaaaggta aaattattct catattgttt atcaaataac attgttacaa aattaacaaa
    55441 taaatttgaa ataataatac tattttaaat gtaaaaatat ttataaataa gcagaaataa
    55501 actatgtaaa agaaaaaagt ttaaaaatca taaataacaa aacaaatata caataaatct
    55561 tatttgaaat tccatttttt gaataactat atatttattt atccgatcaa tgatgtaaat
    55621 tatcaattac agattagatc ttaataaatc taagtattta aataattttt tatattaatt
    55681 ttaaatttta attttgcata attaacttac gaatatatta agggaaaatg aaatattcaa
    55741 aaaaaatcaa atctacagat atcattatag acgaaaaaaa acaatttatc aataaatata
    55801 actttgtcat tattcctata ttattaatat ttttaagctt ttcacttagt aattgtaaaa
    55861 taaaagatac taataaaact ttgcaagcct cagtagagat acaaaatctt ttattagatg
    55921 cacaaaaatg tttactacta aatgagttag atcaagcaaa agagatatta tttgacatta
    55981 tcagtaaaga tcctaagcaa gatgttgctt attatctact tagtaaatat tatgagctaa
    56041 acaataaatg ggatagcgca aataaatata tcgatattgc tatcaaatta tcaccagaaa
    56101 atgtttatta tttagaacta agtgccaata tactggttaa attaagaaaa taccgagaag
    56161 ctgcacagcg atatgagata ttaatatcta agaatcctaa aatattagat tattatttaa
    56221 atgctgcaca aatgtattta atagctttta tgccaaataa tgccttagaa atctttacga
    56281 aattagaatc gcagcatggc tttataccgg aaataggttt ttataaatat caaatatact
    56341 cattgctagg taagagtgat gaagctctag atgagctatg gagatgtata caatatgatc
    56401 cgtctaattt ggattacaaa aaagaatatt cctcatttgc aatagaaaaa ggtaaaataa
    56461 cagctctcta ccgtttagtg caatggtata aacaacgtga tagtaccgat gggtatgcag
    56521 cgctattcgc tgctaattat tattttgcaa ttaataaagt agaagagtta aaaaaagaat
    56581 tgttaatagc tatcaaggac ccaagagtcg attttcaaga caaactgcaa gtgatattaa
    56641 caagcataga aaaccccaat atagatgaac aatttataca acaattattt tatcatttag
    56701 aagatttaca tagcgataaa gccgaaactt ttaatatcat aggtttttat tatcaagcac
    56761 aaaacatttt agacactgct attgtttatt tcaaaaaagc tcttgagtta gactctacta
    56821 atgacgcgac atatagaaat ctcattatgt cttatgaagc taaaaatgat tatgatagta
    56881 tgctatttta tgctaataaa gcgcttaatt tttaccctga tataggcgaa ttttactttt
    56941 atgcaggtta tgctgccttt atgcagaaaa aatttgaaaa gtgcattcca catttagaaa
    57001 acgcactcag ctatatacct tctaagaata aagaattgca aaatattatt atagaattac
    57061 tagctaacgc ttactatgaa acaggagaat atgccaaagc attcgattat ttcgaaaaag
    57121 caataaaagc taatccagcg aatctcgtac tcaaaaataa ttatgcatat tttttggctc
    57181 aacaagatac tatgatggat aaagcattaa atttaataga tgaggtttta ctaacagaac
    57241 ctactagatt ttcttttatt gatactaagg cttggatttt atttaaaatg aaaaattatg
    57301 atgaagcaaa aaaatggata gataaagctt tgatgtataa tgattcggat ccagaaattt
    57361 tagaacatgc aggtgatatt tattatcatc taaatctaat agatgatgca gtgcaactat
    57421 ggcaaaaatc taaggaaaat ggaaataact ctgagataat caataaaaaa ataaatgata
    57481 aaaaatatta tgcgttttaa atttatttta tctattttga tagtttctct tgtttttttt
    57541 agttgcaaaa cttctaaatc acctcagcaa tactctcagg atagtaaatt tatatctata
    57601 gagtcaaaag acacctttgt attagtaaat aatactagtt ataataatat atctttaaaa
    57661 actaatggta tttttaatta tgaaaataaa gaaatcaact tcaatgcaaa tatcatcata
    57721 atacacgata gtttagtatg gatgagtgcc tcagttttgc cgggaataga gatatttaga
    57781 attttagtaa ataaagatag catcaaaatt attaatcgat ttagtagaga atattacaca
    57841 gactcatttg aaaaaattta tgaaaaaatt ggtatttgta taaattttga tgttgtagaa
    57901 gcattattgg tagctaatga tttcatttct tttaaccatg aaattattca aaaacacact
    57961 gattcattat ttactaacta tactatatct agaaatatta ttatcggtcc tgatgataag
    58021 aattgtaatt tattacaaaa tctaaaaatt tctaataatt ccttaaagat agtacaaaat
    58081 gaactaaatt attggaatag agacaatata agtatctcga ttaaatatga aaaatatgat
    58141 ttaataggta ctatgttatt gcctactaat attaatatag aattaaaatc ttacgaaaat
    58201 ccattaatta tagctaaggt gctctttaaa caaataaaat taaatcaaaa tgtatctaca
    58261 ccttttaatg taccaaattg gggtaaataa ttttaacaaa tagcctataa caaacaacat
    58321 ataaatctaa acttataact ttctaaaatc attccccttt gctttttatt ttatattaat
    58381 ttttttaaac atttctaata gctacgaaat ctttatctat tcttttgata ggtattctag
    58441 gcaataaaat agcaatagat aaaaggagta agataatgaa aaaataatct ctaactttga
    58501 atgcaatagg gtaagctata tgtcctacca tatcgcctcc atgcataggt acaatcccaa
    58561 attgtaactg taaaaaagct attatactac caagtaagag acctatggct ccacctccta
    58621 atacaatcat agcaccttct ctcaaaaaaa gtttttttaa ttgcgaaata ttcataccta
    58681 gagcataaag tgtttgtaca cgcttttgtt tatctaacaa taataacccc agtgagctaa
    58741 taatatttaa tgaagctatc aaaacaataa acgctaataa tataaaaact atcaagcgtt
    58801 catttcttaa gattttaaat aaaagttcgt cttgctcgtg tctatctttt actataaatc
    58861 cttcaggcaa taaatttgca agttcttttt tcactttatt gatatttgat ttatccgaaa
    58921 tgcctatttc tatattagaa aatttattag ctattccaag taaatattgc acttcatcga
    58981 taggagcata aatataaata ttatcataat cttgattgat agcaaagata ccagaaatca
    59041 tacatgctga ttcagtgaaa agcgtagtag ctgatacctg agaagaactt tctttaatag
    59101 aatatatata caaatacttc gaataatcct taatattaac attcagatta tgggctactc
    59161 cactacctaa aattgctgct gggatagagt cgtacaagat tacctgaggc tcaccatata
    59221 ataaatatgg ggcaatattt tttgaattgt aaccattagg atttattcct ttcactttaa
    59281 cgactaactg ctcatcatca tatcgggcaa gtgcagtggc ttctaatgtt ggttctatag
    59341 atgctatacc atttatttgt tttatatagt ttagatccaa ctcatttaaa tcaaatggtt
    59401 tgccatcagc tttagagata ctaatatcgg gataaattac ctctgtcata gagactatta
    59461 catcttcgaa gccattaaat acacagatta ctattatgag tgcagctaca gctatcatta
    59521 taccaaaaaa agtaacatat gtcaaaatat taattaagct atatgatttt ttagatttcc
    59581 aaaatctata agctatccaa taagagagca aagaagactt cattatttat ttaatatatt
    59641 gtcaattttt tcgatataat ctaatgaatc atcaataaaa aattgtaaat cgggcatcca
    59701 tcgaagttgg tgtctgattt tttgaaccat taaattttta atttgtggtt ttagaatttg
    59761 tatttctttt aaaacctcat ttttatcagc tatgccataa atagaaagat atactttggc
    59821 tagctttagg tctgcagtta ctcgaactac tgtaactgta atcatagcat ctccgaacca
    59881 ctcttttgct tcattaacaa aaatctctga taaagctctt ttaattacac tagctacgcg
    59941 ttgttgcttg atggtctcca tattaaagat ttattattat taaaaaaaat tttttttaac
    60001 aaaattaatt atacttattg aattaattat taggttaata atattttagc aatatccttg
    60061 ttgcatacat aaatcaaaaa tatatattta tgatgatagt ttcactacat agacattttt
    60121 aattatactg ttcataacga tacttttgga aaatttctaa ttataaaaat aactttgcag
    60181 taattttttt agtttttttg agatatctaa tcgcaaaata agtaaatatt aaaacaaaaa
    60241 taattgaaat aaaatatggt agtgagttta taaaattatt aagataaaaa acataataca
    60301 ttttcattat taataaagta gctgctaata aagctataaa agtagttgta tattctttaa
    60361 ttaaaaccct tttataatta aattgtgtgg aatttataat ttttgaaata tttttcatat
    60421 taggaatcca tctattaaca ttttgcttat attctaaata ttcattacca aattttttta
    60481 aaagaaaatc ttcttcagct agaacaatgc aataataaat aaaaacaaaa agtgggaaca
    60541 taatgagtaa aaaaattaat gagtttgata aaaatgccaa gccacatatt tcaattatat
    60601 tccctagata aagtggattg cgacatagtg aaaaaagtcc atcagtatgc aatttgtcag
    60661 catatatata tttattttta cctgccctat taatgttgac taagcctata gtaaagtatc
    60721 taataccaag tcctataaaa agcaatatta atcctaaaat caatggtata gtaaatgaag
    60781 gaaaaatttt aggtgatgga ataaataaaa taaaataaaa gaatgggaaa atataattac
    60841 gataatgaaa aaataaggaa ccaatttttg tttttattga agtttttgga tttatttgct
    60901 ttttttcgat gaaattatct gtgcttttgg ttttacaatt tttattattt tttgacataa
    60961 aacattaaat tttatacaaa aataatttat ttaaataata tagagattat tatatatcta
    61021 taagatcttt tacctaatgt taattaaatc aataaaatca gagctgtttt tgttatgtgt
    61081 tattattgtt aatatttagg taatagcagg aagcttattt taataaaaaa taaagggaat
    61141 tgaatagttg taattaaatg tttaagtttt tcaagtttat atgttaaata atttttatat
    61201 ttttacaaa
//
LOCUS       DAOPRN010000005        58024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_403_length_58024_cov_125.613362, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000005 DAOPRN010000000
VERSION     DAOPRN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 58024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58024
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_403_length_58024_cov_125.613362"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(473..2287)
                     /locus_tag="PKI65_01150"
     CDS             complement(473..2287)
                     /locus_tag="PKI65_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOB77078.1"
                     /translation="MFKNHPKGLVIAFFSNMGERFGFYTMMAILVLFLQAKFGMSAES
                     AGSIYSWFYFAIYALALVGGIIADTTKRYKLVVLLGLIVMLLGYVMMAVPGTTLAIAI
                     TGLMVIALGNGLFKGNLQAIVGQLYDDPKYEPLRDTGYLLFYMGINIGAFFAPFAATG
                     IRNWFLKINGFIHDASLPAMCHAYLDGQSLDIAKFQDLANSVSSTVVTDLSAFAHQYI
                     DVFSRGYNYAFGIAAIAMIISLLIFVFYQKSLPDARKIQKAQKEQEAKEHKEQPTTEK
                     FPIKNLIYAIVVFVAVLGIFWFIMKNVDIGFAVALFCGFITYIILSSTPEEKPKITSL
                     VFVFFVVIFFWMSFHQNGLTLTLFARDYVFKEVNAFTALFFNLKSLLSIILAITGIVM
                     IFTSKKLVNKIIAAAFFLGFGYLTYYYYQINVGKMIPIAPELFQSFNPLFIVALTFPI
                     NGLFSWLRNKGKEPSTPKKIGIGMFIAAFAYTIMIFASANLTSPSEIAQSSITDYERV
                     TPYWLISTYLMLTIAELFLSPMGLSFVSKVAPQRFQGLMQGGWLLTTAVGNKLLFLGT
                     YFWERIELWQLWLIFVGVCVASGLLIFALMKKLEAATS"
     gene            complement(2302..3723)
                     /locus_tag="PKI65_01155"
     CDS             complement(2302..3723)
                     /locus_tag="PKI65_01155"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOB77079.1"
                     /translation="MIKQKTLKNEVFIEGKGVHIGKNVKLTLKPNDENTGIVFKRIDI
                     DMNNTIPALADYASDTIRGTNLSRDGVVIRTVEHLLAAIIALDIDNIIIEMDNEEVPI
                     LEGNSYGFINAIKNQSEIIEQNENRKIYNISTTLSYYDEEKSAEYWLTPDDNYRLTVM
                     IDYNKDILPPQYATLNDMNEFEKEISHARTFIFLSELDELIQHGLIKGGDLNNGLVIV
                     DKQIPQEKLDFYKDYFNIHNVTIIDKGILNNTDYYYSNEPARHKLLDVVGDLALVGYR
                     FNGHVIANKPGHKSNVEFAKIIREQLKKEAKKSPVSNFDIYNKEPLYDIEAIKHYMPH
                     RYPFLLVDKIIEKGKNYIIGIKNVTYNETHFLGHFPKQAVMPGVLLIEAMAQTGGMMI
                     LSDLPDPENYITYFLSIKDVKFRHKVVPGDTVVFYLELASPIRRGIVNMIGKAYVGEK
                     IVMEANMMAQVVKMENPYLETNK"
     gene            complement(3695..4762)
                     /gene="lpxD"
                     /locus_tag="PKI65_01160"
     CDS             complement(3695..4762)
                     /gene="lpxD"
                     /locus_tag="PKI65_01160"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOB77080.1"
                     /translation="MKFTISQISQLLEGEIEGDDTIEIRNVARIEEAKKGDIAFIFDP
                     KYEKCIYDTQASALIISKDLALIDPVNVTLIRVNNPYLSFLKILNIIDEAKKKRKNYT
                     DISKFAYIDEEATIGNNVSIGHYSVVCKNSVISDNTILREQVYIGENVHIGKNCYIYP
                     QVVILEDCEVGDNCIIHSGAIIGSDGFGFFPKEDGTYEKIPQIGNVIIENNVEIGANV
                     SIDRSTLGSTRIKEGTKLDNLIHIAHNVVVGKNNAMAAQCGIAGSTKIGDNNMFGGQV
                     GISGHISIRNNNKIAAQSGIASNVSDNEMLMGSPAIDAKAFKRNFIAFKELAEIRNKV
                     YNLDKLISNLIKNDKTKDTKK"
     gene            complement(4790..5326)
                     /locus_tag="PKI65_01165"
     CDS             complement(4790..5326)
                     /locus_tag="PKI65_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77081.1"
                     /translation="MKKNDFLHFLLLALLILLVVVYRLFNFIPNFTPIAALALLCGYV
                     FKNKKWYLILPVIALFISDLFIGLYQSIVMIFTYLSFVVIALIGAKMIKKIKVTSVFL
                     SSITASFTFFVLSNFGVWLGGWYGYSWHGLINCYIMAIPFFRYEIIGTLFFTFLIFAI
                     FNFAIKPIATPELQNKLY"
     gene            5606..6592
                     /locus_tag="PKI65_01170"
     CDS             5606..6592
                     /locus_tag="PKI65_01170"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77082.1"
                     /translation="MDNFNLLDYQALPKEQKRRFLDNLYQFLLENNYTAVDYQKLKIQ
                     STAPICPRCESENVIKAGVRDGKQVYKCKDCGRQYRETARTFVYRMRKADKMLDYLKC
                     MLEGKSLRACASEVGISLRTSFNWRHKILAAIKSLQGGISFSGIVEADELLMKYSEKG
                     RKYRSRKEYEKAKKKKHPKVAVLVMTDREGNLLFKHVGRKKVTNSQIREELSHRVTDS
                     NLICVKQKKEFKKAVKGTKSKKVVVNKTQIRRGVYSVQVVDKHINDFKVWLRRFRGVA
                     TKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSDRWAWDEYWRLVNTKYR"
     gene            6873..7055
                     /locus_tag="PKI65_01175"
     CDS             6873..7055
                     /locus_tag="PKI65_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77083.1"
                     /translation="MRRFRGVATKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
     gene            complement(7229..8452)
                     /locus_tag="PKI65_01180"
     CDS             complement(7229..8452)
                     /locus_tag="PKI65_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOB77084.1"
                     /translation="MTEIKLPELDDYDYKFSNEKIAKFPVEPRDSSKILIGNNENLQF
                     DYFYNIINYLPENGLLILNNTKVVPARIMVKKETGAWIEFLLMEPVNADSIAQALQDI
                     GMSKWKCMIGNAKKLRNSPHLSYYFTKNNQNIYIQIDVEWLENNENIVTFKWQPEGFS
                     LSEILELIGEMPIPHYIKRKPVEDDKIFYQPVFAKVDGSIASPTASLHFTDRVFEKFE
                     NKGYQKIFVTLHVGAGTFKPVKTSIEEHQMHREEFLVNKSTIDVLYENINRPWVVVGT
                     TTLRCLESLLLFAENLYPYPININQFDIMHRKYNKICDRKILIEKLKKFLENKNFDNL
                     YGHTSLFILPPATVLSADYLITNFHQPKSTLLMLVDAFSSISWRNIYDYALENDFRLF
                     SYGDACLLKNKLLDK"
     gene            8582..10213
                     /locus_tag="PKI65_01185"
     CDS             8582..10213
                     /locus_tag="PKI65_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOB77085.1"
                     /translation="MKTKIFPIFMIVIFFINSGCKKKTETVEAILPIDSLAIYATYEL
                     KTDLSHLTDAEKQAIKLLIKAADIMDELFWHQAFGDKNLMDTISNDTLRQYSYINYGP
                     WDRLNNNKPFISGYGPKPDGANFYPADLTKEEFENWQNTDKTSLYTMIRRDANGNLLA
                     IYYHEYFKEQLSKAADLIEQAAKLINDKNFANYLSLRSEALKTSNYYASDMAWLDSKT
                     SKIEYIVGPIENYEDRLFGYKAAFESFVLIKDIEWSKKLEKYGKLLPQLQKSLPVNDE
                     YKKEIPGSMGDINVYDAIYYAGDCNAGSKTIAINLPNDEEVQLKKGTRKLQLKNVMQA
                     KFDKIVVPIAELILDENIAGNVVFNAFFQNVMFHEVAHGLGIKNTINNKGTVREALKE
                     AYSPIEEAKADIMGLYLIEKLREMGEITEGLLEQNYASFVASIFRSVRFGAASAHGIA
                     NMLEFNFLVDAAAISKNSQGKYNIDFEKMKNAISDMVQKIITIQANGDYESAIQWINE
                     KGKINTDLQKDLNKINDAKIPVDIVFKQGIDVLGL"
     gene            10409..10633
                     /locus_tag="PKI65_01190"
     CDS             10409..10633
                     /locus_tag="PKI65_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77086.1"
                     /translation="MINIRPNYNIMPLKELEQYILQNKHLPDVPNQDEISKNGMYVYE
                     MNTVLLKNVEELTLYVIELEKRIDEMEKVK"
     gene            10645..11493
                     /locus_tag="PKI65_01195"
     CDS             10645..11493
                     /locus_tag="PKI65_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77087.1"
                     /translation="MKQIILSFILIIYSINTFPQYINYNLTNQSETLLSSNNELNLSK
                     MNDTSAFYKSELGFTTGFNWFSLFTPKKENKEYGDLDYLYYYDNYIASVFYIRNYNQI
                     LSLGLEIANTATKLKTKSADVIGAMGHKYQDLDYNINYLNFILLLQFNIFSKNKIDIN
                     INLNPYISYIIYSKVTGYIESWSTIILQDSLGNLFSRPSHSIINLNESPIKDINKLLR
                     GFRIGYDIEYAYKPNVSFLFANYFCYTTSTNISCSNCEGKWRSIAFSLGIIYKFNKSY
                     EKNNIN"
     gene            11471..12136
                     /locus_tag="PKI65_01200"
     CDS             11471..12136
                     /locus_tag="PKI65_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77088.1"
                     /translation="MKKIILINFIILLSLFKIYSQDKTFLSTEFNTNYFYYFLGENNN
                     KFNYGFSLQLSENINKFKISFGAIYATKYYWDKPSEKYNKCEYLLKNIRLQIIGNYQI
                     ISKNTFSMDILGGFDFDQIIDYDVKLYYVNGYREKINNIKIQNKLGVSCVMGLNFSKL
                     ISDRVALNLSPKFYITIKQDHEPYSPPLGPISEPFYENLIDDLFTFNLSIGIEYLFKK
                     SEQ"
     gene            12460..12936
                     /locus_tag="PKI65_01205"
     CDS             12460..12936
                     /locus_tag="PKI65_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77089.1"
                     /translation="MFIITGMTEVAVDTKGRFLMPAFWKTLFTQYQINEFVIKRHLYH
                     SCLELIPISVWNDELEKLNKLNRFNKQSNDFIRFYMAGVRVIEFDKVGRIQIPKDLYD
                     FADIKNTAVLSSSIDRLEIWDKEQYENYLNKHSNEFQKFAEEFLGKSFMSDEEDKS"
     gene            12933..13844
                     /gene="rsmH"
                     /locus_tag="PKI65_01210"
     CDS             12933..13844
                     /gene="rsmH"
                     /locus_tag="PKI65_01210"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOB77090.1"
                     /translation="MSNQGYHIPVLLKEVKNEVPLLDRGIYVDATFGSGGHSQAILSI
                     ISKKSKLFAFDIDVDALTNDINDKRFTLINANFKFVRFYLNYFEALPVDFILADLGLS
                     SHHIETDIRGFSFMSDVPLDMRMNQEQQINAKEILNNFSEDELIQIFRNYGNVPFAKS
                     LAHDIVNSRKTKPIKTSSELNDLALKYILKGKEFKNLAKVYQALRIAVNNEVDNLKML
                     IDEAYKSLNPGGKLCIITFNSLEDQIVKNELRKKIDNLLDQNWLKGTKHQKWSKIYKI
                     TPNDEEIKSNPRARSAKMYVAIKNENL"
     gene            13841..14239
                     /locus_tag="PKI65_01215"
     CDS             13841..14239
                     /locus_tag="PKI65_01215"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOB77091.1"
                     /translation="MSKNKSKKDKKDKKNIIKALMSGDYLASDPLIKQLPFIAWITFL
                     MILYIAKNYQIDKTLYELGKIKNENEALKTKSIVLNARKIATMNLEYCYKELAKDGLK
                     PSDQPPIKIFDYSIEINNKKIKKSRKNASR"
     gene            14226..16349
                     /locus_tag="PKI65_01220"
     CDS             14226..16349
                     /locus_tag="PKI65_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOB77092.1"
                     /translation="MPQDKIPFDEIAKSKIFGRASVVFFIVVFIGILIIYKIIDIQFV
                     RGDELRSHTFDYEYKYVEITGIRGNIYSEDGMVLATSIPVYDVYLDLNEKNVSKDLFD
                     TQIDSLSLKLANKFRDKTANDYKSSLIKIRKSGKRYVKFKSNLSYEDVEELKTFPIFS
                     RGQYGGGLILVKKDRRVKPLGSIAERTIGFSRDNFKVGIEGAYDSILAGNKGYQWMHR
                     IAPGVWIPAEDTSSTIKPIDGKHIKTTINAYYQQIATRALEKVLKENQADHGTVLLME
                     VATGKIKAIANLKCMPDSSYKEVYNYAIGERYEPGSTFKTISLLAAIDDGLIDLDEII
                     NTGSGTWRYGSQVMSDSHDGGFGEISLRKCFELSSNIGISKAIIKAYSSDEKKFFDKL
                     ESFGITHKTGIDIKGEPNPSVNFPNSKLYSRYTLAWTSIGYEVLLTPLQILTFYNAIA
                     NNGKMMQPFLVDEFIDENRTIWKKEPKVLNKKIAKDASIKTMQSLLEGVVQHGTASNI
                     KDSIIPIAGKTGTALIAKDGGYKSEGKEYVASFAGYFPANNPLYSCIVVVNKPKQGLY
                     YGSQIAAPVFYEIAKKIYSTQLEIYSIENKETFNNNLYFFTPAQLIKNVQSNIKVTTY
                     PFYTNDGFARLDTNKIDVIATSHFINNQKLPDFKGMLLHDALFIIKKLGLKVEIKGAG
                     RVIDQKPAPLTNIDKNLDKIVLFLK"
     gene            16360..17814
                     /locus_tag="PKI65_01225"
     CDS             16360..17814
                     /locus_tag="PKI65_01225"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOB77093.1"
                     /translation="MKNLKDILHSIEVESIKGDINLNIDNLSNNSNTIKANGLFIAKK
                     GYKVDGHDFVNNAINNGAVAVVCSENIPIPENITKILVKDSTDVEGKIADNFYDHPSK
                     KLKVIGITGTNGKTTTATLIYQLLNNLGKKAGLISTITYKYGNVELPSINTTPDAIEL
                     NRLLNEMWRNGCKYAIMEVSSHSIVQKRILGINFSGAIFTNITHEHLDYHGTFENYIK
                     AKKTFFDNLPADAFALINIDNKNSKIMVQNTKAQVYTYGLAKMADFHARILENTLEGL
                     HLEIDGYDTWFLLAGKFNAYNILAAYGCTKILGYDKQDILSELSKVEPINGRFNVINN
                     DGVYIIIDYAHTPDALENILNTLNEIDIDGKIITVFGAGGNRDKTKRPLMGKLAAEYS
                     DIVIITSDNPRNEDPEAIISDIEKGVPTNLNSKVYKITDRADAIKMAINLANKGDVVV
                     IAGKGHETYQEVKGIKHHFDDKEIVFQIINSKKQ"
     gene            17834..19093
                     /gene="mraY"
                     /locus_tag="PKI65_01230"
     CDS             17834..19093
                     /gene="mraY"
                     /locus_tag="PKI65_01230"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOB77094.1"
                     /translation="MLYYLFTYLDRIYDFPGAGLFNYISFRAAMSALFSLLIILIFGK
                     PFINYLKKKQIGEEIRHLGLEEEDRKKGTPTMGGILIFLAIIIPVLLFAKLENVYVIL
                     MLITTIMMAGLGFIDDYIKVFKKNKKGVSEGAKIIVQLALGLIVALVMYNNKHVAIRE
                     QISQTNIVADVQEAPVMRTASDYFGPEQIGSLKTTIPFVKNNQFDYANLVKWIGEDYQ
                     KWAWIVFVPIILIIVLLVTNGANLTDGLDGLCAGTSAVVGAGLAVFAWVSGNFVFASY
                     LNILYLPNSGELTVFIASFVGSLIGFMWYNSYPAKIFMGDTGSLMIGGIIAVFAILVR
                     KELLLPLLCLVFLLEIGSSAIQRYVFKYRKWRFGLEYAQKNRVFLMAPLHHHFQKKGW
                     HEVTIVNRFIIINIIAAILAVITLKIR"
     gene            19096..19659
                     /locus_tag="PKI65_01235"
     CDS             19096..19659
                     /locus_tag="PKI65_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77095.1"
                     /translation="MKKVNNKMTYILNDIISKSNNISLAENIPHLLDDQLKSSKKLSK
                     NYLKYFTKNTNRLVVLRKLNNVKFIDDSYAINNYALCYALEMTNDPIIWISLDNSNIY
                     LSKYLTENIEKKVKIWINISKNNDIHNSIYAYLVPEFYTVKNLSQAIKLAYAFADPGD
                     NILFAPNKSNIISKTYSNKFKKIVNEL"
     gene            19656..20852
                     /locus_tag="PKI65_01240"
     CDS             19656..20852
                     /locus_tag="PKI65_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOB77096.1"
                     /translation="MSKGIFKIFKGDKTIWIIYIFLCIFSMIAVYSTIGNLAFRFNEG
                     NTTYYLIQHIIILIFGIVLAYLVHLMDYRIWGFLARFLFLMAVPLLIATLFKGHTTND
                     AARWLRIPVIGISFQSSDFARFALIILLARIAASKQDNIKDFKEAYLPMIIAVAITVG
                     LIFPTNLSTAILILATALLIMYIARVPLKYLIVTIGIAVVIMGISILIMLQMPNKGRV
                     GTWQSRIESFTNKNGSVPYQRMKADIAIANGGIVGKFPGNSTQRGTLPQSHTDFIYAI
                     IIEELGIVGGIAVLLFYCVLMYRAIHVGKQSKGFFGAFLAIGFGFYIGFQAIVNMSVA
                     VGLIPVTGQPLPMVSKGGTSIIFTSIAVGIIQSIARDCQKDQKQIDEQMETDDNEIIE
                     NISEAG"
     gene            20874..21986
                     /gene="murG"
                     /locus_tag="PKI65_01245"
     CDS             20874..21986
                     /gene="murG"
                     /locus_tag="PKI65_01245"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOB77097.1"
                     /translation="MKIKNEIKKWVIFSAGGTGGHIFPALAVAERLRELHPELGILFI
                     GNKARMESEIIPKYDFPIILLDMHGLDRKNILKNINLPIKLIKSWIKLNKIFKKFKPK
                     VVIGFGGYVTYPVLKLAQWRGIPTIIQEQNSTPGLANIKLSKKASAICAAYPTIKNYI
                     KTPNVYMIGNLVRQEILLPMSKEEAANFWNLDINKKTILALGGSLGAKAINDFICNIL
                     TDLISKDLQLIWQTGDKYSINSCLNIDKLNDNIIVKPFIREMREAYTMADIVIARAGA
                     GTLAELSALAKPSIIIPSPNVVNNHQYLNAQALAESNAIILLDENLLDIKGKEILLDL
                     INNENLQKKLSKNISKFHNPNAINDFIELIEKYLKN"
     gene            21992..23353
                     /gene="murC"
                     /locus_tag="PKI65_01250"
     CDS             21992..23353
                     /gene="murC"
                     /locus_tag="PKI65_01250"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOB77098.1"
                     /translation="MKLNNIEVKHIYMLGIGGIGMSSLAQYFLYNGCKVSGYDRVRSE
                     ICNILENKGANIHYEANNDLVPNDINLVVYTPAIADDFTEWEIIKNKKLPVYKRSDIL
                     AMITPQFKTFAVAGTHGKSTVTAILSHIWNTADRDFMSMVGAIIKNYENNFIINGEPK
                     EFIVEADEYDKTFLKFTVDTGIITSIEPDHLDIYKNYEDLKTAFTEFANKTKNEIWLD
                     ENVNLQTNKKNLTYGLSDNSDIFAYNIKLKDSKYIFDVKSNYHNIKIEKLQLNITGKH
                     NIKNAIASIAIALSNNINIDQIKKAISTYPGLKRRLDLIYNSKNKKYYDDYAHHPTEI
                     DALFEALKSIYPDKTLCAIFQPHLYSRTLYLADGFANALSKWDRIILLDIYPAREEPI
                     DGVTSEWLLSKIDNKNKYLMTKDELLNNIEKINEDIIVTIGAGDIDRLREPIKNKLIE
                     YET"
     gene            23343..24140
                     /locus_tag="PKI65_01255"
     CDS             23343..24140
                     /locus_tag="PKI65_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77099.1"
                     /translation="MKHKKWLIACSIILPIIIIIGWLFIRDKSEATSIARIDINIHTA
                     DTNKVIDTTYVLDLIYQVTPRVIGMVIEDFPLDNIYQTIKKSPFIEKLLCNITLDGTL
                     KIEAWARCPLLHIISNNGYECILDTSGYLMPVPTRYNLYIPVAYGNIKTKLKIGTKVQ
                     NIQSYTYESKGFTELDKLFYLAKNIKNNKISNLLFWDIYVENPKSFYLYPLVGNQYIK
                     LGDIDQLNEKLKNITIFYNHIHQIANVDEYEGFDLRVPPQVIAIKQL"
     gene            24177..25466
                     /gene="ftsA"
                     /locus_tag="PKI65_01260"
     CDS             24177..25466
                     /gene="ftsA"
                     /locus_tag="PKI65_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOB77100.1"
                     /translation="MKKQVDTLEERIHVGLDIGTTKIAMVAGRLNGDGKLEILGHQIV
                     QSKGVRRGTVINVQKTVDGLKELKMKMLKDFGIEVGIVHLGIAGKYIESLNIEHSIIR
                     KNPNNMITGQEINDFLETVKQMLKQPGRDVIHIIPQEYTINDESGITDPIGAVGSRLS
                     CKIHAAVANISAIEMLKNCVSMADMDIADIMLEPLASAEAVLEEQEKEGGVVLVDIGG
                     GTTDIAIIKENIIRHVCVIPFGGEAVTEDLKNGLSLLKEEAELCKVNYGNAIPADVNP
                     NEVIAFQAMKNCPQREFSLRAISEIISARMSEILETVNYEINISNVTMPLIGGIVLTG
                     GGSLIKNTSQLSSYITGLPSRVGTPNHLLSHDTSSYLLSPQYSTAIGLLLNAIKNDYN
                     QIILNNRVNENKVDGIKEKKQKKYKFDIWGNLSNFFD"
     gene            25487..26698
                     /gene="ftsZ"
                     /locus_tag="PKI65_01265"
     CDS             25487..26698
                     /gene="ftsZ"
                     /locus_tag="PKI65_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOB77101.1"
                     /translation="MDKKSFEFILPASDPPIIKVIGIGGGGCNAVKHMFDKGIKGVDF
                     VICNTDLQVLETNPIPIKIKLGASGLGAGSKPEVGKKAAEESIEEIRNLLKNNTKMLF
                     ITAGMGGGTGTGAAPVIANIAKELDILTVAIVTTPFMFEGNIRYQQAMTGIEELRNNV
                     DALLVIHNEKLFELYPDLKISNAFSKVDDILLTAAKGIAEIITVPGIVNVDFEDVKTV
                     IKDSGIALMGTGYAEGENRAIESLESAIHSPLLSEIAITGAQNVLLYISYGSDELTIK
                     EFKEMTSYLQNETGGANVIWGNSHNPELNGVQVTIIATGFNPTQSTTIKSQKYENTYC
                     DDSKVAEQKIGYGRNYRDDEELEKLENIPAYIRKGKKININAPSPGNIAININKDKTI
                     SENNYLYKHVD"
     gene            complement(26827..26973)
                     /locus_tag="PKI65_01270"
     CDS             complement(26827..26973)
                     /locus_tag="PKI65_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77102.1"
                     /translation="MMSNKVNSTQAEALTMVCAQVIGNDVSINIRGMNGYLQLNTIMP
                     MINR"
     gene            complement(27032..27400)
                     /locus_tag="PKI65_01275"
     CDS             complement(27032..27400)
                     /locus_tag="PKI65_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOB77103.1"
                     /translation="MKRTLNINYKYRYKNIIKIGRTHTMDAVPLALRQEFSGYVSQLE
                     HNIQKLEHSLDHLSELPIGGTVVGTELNAPKTFDVIVYKKIAEFTELPFKPAKNKFEG
                     ISTCDAIVSASEALKSTAVS"
     gene            complement(27493..28203)
                     /locus_tag="PKI65_01280"
     CDS             complement(27493..28203)
                     /locus_tag="PKI65_01280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOB77104.1"
                     /translation="MYKDHLESFLKYNERIHDIKDSRQETDENNPAWNNGFLPGLDIV
                     GIYGIIAKYKPAKYIEIGSGNSTKVAYKSIKENNLNTEIISIDPLPRANIDHLADKVI
                     RKPFENLPDNKFIVKSLEKNDILFIDNSHRVFPNSDAMICFLELLPFLKNGVIVHIHD
                     IYLPYDYPQFMCARFYNEQYMLAAFILANPEKYKTILPNYFISEDVELAKIISPIWNH
                     PNLSGVERHGCSFWLQIS"
     gene            complement(28227..28352)
                     /locus_tag="PKI65_01285"
     CDS             complement(28227..28352)
                     /locus_tag="PKI65_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77105.1"
                     /translation="MKEKIKSVYKFISPKFQSIHLEYKVIPKPRYGHGNPPIPFY"
     gene            complement(28411..30465)
                     /locus_tag="PKI65_01290"
     CDS             complement(28411..30465)
                     /locus_tag="PKI65_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOB77106.1"
                     /translation="MKIILPYSPWWLLLFAILSIALALLLYFKNPYDALSKPWKNTLI
                     VTRSIVFFLLFFLLLTPYIQKNKKIVYPPEIILLVDNSQSITVDNSFNEISTKIKSLT
                     DKYSKNYIWHNYSFSNVITNKLLFTFDGKATNLYDALQLAINKSLENDNIRAIILVSD
                     GINTQKQDPRFLFNKFKNPIYTIGLGDTTTQTDLQVSKIQCNEKIPIGSNFPIEIILN
                     AKKAKGKTSKLQIFIDDELWYSENINITHDNYSKILTIQAKAEKEGIHKIKVTWQQIS
                     EEKNIKNNNLSKSIEVVKQKYKIGILACATHPDIGAINRALSSVLLNEVQLIRPGIDP
                     IANDLDVLIIYQLNSACINTSKIEELKQSTIPIWLFIGTQTQLPLATSIVPWIGNLIN
                     SSYIESQVKVNEQFPLFQIANEELHFFEQAPPIITLYPRNRTSIKFPTIFYQKIGTAT
                     TTNPVMFFYQSINKNIAVFNGEGIWRWRIESLRNNNNNFDTWVIKVINYLLTTTKEGF
                     KIIVESVYQSVDPINIYAELINPSGELINTPEIQIIINSNQGYSNKYLFSRSGNGYSL
                     QLGQLPPGVYNYSATVLSPDFPFTAKGFFIVEKIDVELQDLTANHDLLKEIANASGGK
                     FLYFSELDSLEKILKSQTMSQKYYTIEKITSSVIDSWIYLVIITLLLGFEWFIRKYMG
                     GY"
     gene            complement(30469..31218)
                     /gene="fabG"
                     /locus_tag="PKI65_01295"
     CDS             complement(30469..31218)
                     /gene="fabG"
                     /locus_tag="PKI65_01295"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOB77107.1"
                     /translation="MKLLENKTAVITGAARGIGRAIAEKFASNGANVAITDLNINEEV
                     EKFIDEIQKKYNIKAKAYAFDVSDLNACQDFVEKLLQDFTTIDILVNNAGITRDNLLL
                     RMSEQDWDLVIKVNLKSVFNMTKVLLQHFMRNRSGSIINMASVVGVAGNAGQANYSAS
                     KAGIIGFTKSIAQEVGSRNIRCNAIAPGFIETPMTAQLPDEVRKDWMQKIPLRRGGTP
                     DDVANIALFLASELSSYVSGQVINVCGGMRM"
     gene            complement(31266..32966)
                     /gene="ftcD"
                     /locus_tag="PKI65_01300"
     CDS             complement(31266..32966)
                     /gene="ftcD"
                     /locus_tag="PKI65_01300"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOB77108.1"
                     /translation="MLLNKKIIECVPNFSEGRDLNIINQITEEIKNVEGVKLLDVDPG
                     ATTNRTVVTFAGEPDAVIEAAFRAIKKATELIDMTRHKGAHPRFGATDVCPLVPVANI
                     TMDEVVEYANKLGKRIGEELGIPVYLYEFAAKTEERRNLANVRAGEYEGLEKKLKDPH
                     WHPDYGPANFLPKIGAIAVGARNFLVAINFNLNTTSVRRANAIAFDVREKGRPMRKGN
                     SITGEIVKDENGNTVYIPGTLKATKAIGWFIEEYGIAQVSMNITDITVTPVHIAFDEV
                     SEKARLRGIRVTGAEIVGLIPKSVLIDAGKYYLRKQQRSTGVSENELIKIAIKSMGLD
                     DLKPFVPQSKVIEFILAKDEPPPKLINMSLCEFADETASESPTPGGGSVSAYVGALGT
                     ALATMVANLSSHKRGWDDRWEEFSNYAEEGQKIKEELIQLLEEDANAFNKVMDAFGLP
                     KDTEEQMINRKKAIEEATRYAIEIPYRTLKTASLAIPLCKKMAEIGNPNSVSDAGVGA
                     IACLTAIKGAYMNVLINVAGLDDKTWAKPILDDSAQIIENTEKEVNEIYNFVLNKIKA
                     "
     gene            33517..35265
                     /gene="lysS"
                     /locus_tag="PKI65_01305"
     CDS             33517..35265
                     /gene="lysS"
                     /locus_tag="PKI65_01305"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOB77109.1"
                     /translation="MAIELSEQEIIRREALKQLREMGIEPYPNEAYTVTHYAKDILEN
                     YKEDSTQYGDVSLAGRIMSRRIMGGASFIEIQDSTGRIQAYIKQDEICPPDDNTFYQV
                     VFKKLMDIGDIVGIRGFVFRTKMGEITIHVKELKLLAKCLRPLPIVKEKDGKIYHAFT
                     DPEQRYRNRTLDLIVNPHVRDIFRTRTKMIQSMRDFLNKKNFMEVETPILQPIYGGAT
                     ARPFKTYHNTLDTSLFLRIANELYLKRLIAGGFDAVYEFAKDFRNEGMDRFHNPEFTQ
                     MELYVAYKDYYWMMDLVEEMLEYITLAIHGKTDILVGEHTISLASPFKRICFFDAIYK
                     YTGHDISNLDENELIEVAKILDIELDGVSGKAKIIDTIFGDKVEPLLIQPTFIMDYPI
                     EMSPLAKKHRSKPGLVERFELIINGKELCNAYTELNDPLDQRQRLEEQLQLRQLGDEE
                     AMMLDEDFLSAIEFGLPPTAGLGIGIDRLAMIMTNVPSIQEVIFFPQMAPERKAIAMD
                     SESLKSIGIPDVWIPLLTESGIVTLEDLIETNVNKILNSLGGLRKKHKIKDSLPTKED
                     IEKWIKNAEKSIKESK"
     gene            35600..36364
                     /gene="tpiA"
                     /locus_tag="PKI65_01310"
     CDS             35600..36364
                     /gene="tpiA"
                     /locus_tag="PKI65_01310"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOB77110.1"
                     /translation="MRKKIVAGNWKMNPNYDEAIDLLNFLKDNIPTDHSGVDIIICPP
                     FIYLIEAKKILNRQDIYVGAQNVSSFTNGAYTGEISAAMLKSININYTIIGHSERRQY
                     FGEKEENFSKKIKLCLQEHITPIYCVGETLEQRNNGEQLKVVEKQISEALYNLSDDEI
                     AKIIIAYEPVWAIGTGVNATPDQAQQMHVFIRELIAKKYNQTISQGIIILYGGSVKAS
                     NAKELFSMPDIDGGLIGGASLKGKEFTSIINIAMNI"
     gene            36368..37612
                     /locus_tag="PKI65_01315"
     CDS             36368..37612
                     /locus_tag="PKI65_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOB77111.1"
                     /translation="MIWIFIKEAIAMAWHSLKSNKLRTILTISGITIGIFSIIAVLTV
                     IDSIKNQVNTSIASLGSDALFIQKWPWSFDADMPWWKYVSRPNTSYREYLFLKNYSET
                     IGDITYTALLNTKTSSHNITKSNQSIIGITTSYANFFPFIIKEGRMFTLEEVEQGSSV
                     CILGYEAAINYFGTTKIIGQTIKIGSQKLNIVGVLEKEGENILAGSNDKLIFVPVLFL
                     RNFIKLDSDEANPYISVKPKPNYTQEDLKNEVRFLLRQYRRLPPKKEDDFTINTATML
                     TQSVEGIFKSMTLGGWIIGGFALLVGGFGIANIMFVSIKERTQQIGIQKALGAKNRFI
                     LLQFLFESVFLSIIGGLIGLLLVLILSIVLKFLINFAFPLTFENIITGIIIATVTGIL
                     AGIVPALQASKLDPVEAMRKNV"
     gene            37937..38377
                     /locus_tag="PKI65_01320"
     CDS             37937..38377
                     /locus_tag="PKI65_01320"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOB77112.1"
                     /translation="MKKLSVILLIVIASITISGCKDKDKNNDSQEATKTTLLCKKWKI
                     EKIYMNGQERQDLLNLMSGMVWEFKKDGTFTIYLGTSIASSGTWNFNNDETQINLTVT
                     YEQGENLEEPINDIFYILKLNESQLWVRDDQNSDSFEYHFIPLT"
     gene            complement(38694..40793)
                     /locus_tag="PKI65_01325"
     CDS             complement(38694..40793)
                     /locus_tag="PKI65_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOB77113.1"
                     /translation="MRKLLFFITMFLIMNSTISQELNYPKTKKVDVVENYHGTLVSDP
                     YRWLEDENSPETKLWIKEQNELTFSYLSQIPFRDKLRKYYEEIWNYERMGTPSEIGDK
                     LYYFKNSGLQNQSVFCEKNKKTNEELVLLDPNKWDPKGTTAISALAFSHDKQYLAYGI
                     SKAGSDWVEIFVMDLKTKKLLPDHIKWAKFTSIAWYKDGFFYSGYEITDSTKALTAKN
                     EYHKIYYHKIGTQQNKDKLIYENNDYPLRNFLLYTDKDEKYAFLYENESTSGIQLHVS
                     KLPLKNNSFSLLFKGFEHDYYLVDIINDFAYFITNEGAPNYKLIKISLKKPSLSPKTV
                     IAESKFVLNGVFASKDYWVIQYQEDVKSKLYLADKTGKILDEIKLPTIGTVAGISTYH
                     EHNNIYFTFTSYLYPPTIFYYNADTRQLSLYFKPSLKFNPDDYVTEQIFYTSKDGTKI
                     PMFITHKKDIKLDGNNPTLMYGYGGFNISVLPSFSIKIIPFLERGGIYAVPNIRGGGE
                     YGKIWHEAGIKEKKQNVFDDFIAAAEYLISRGYTSPSKLAINGGSNGGLLVGAVMTQR
                     PDLFKVALPAVGVMDMLRYHKFTIGWAWISDYGSSDDPNDFEYLYAYSPLHNIKEGVE
                     YPATLVTTADHDDRVVPAHSFKFIATLQEKQTGNNPVLIRIETDAGHGGGKPIAKTID
                     EVADVFAFMMFNLGMEF"
     gene            complement(40949..42154)
                     /locus_tag="PKI65_01330"
     CDS             complement(40949..42154)
                     /locus_tag="PKI65_01330"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOB77114.1"
                     /translation="MKSEINKNNIFVEVGFSYRNHYDSYICGDTFLYKRFKEENRYIV
                     VLSDGMGHGVKANVLSALTATMALNFTYEHKEEFRIAEIILNTLPVCSERKISYATFT
                     ICDILPDSTVKILEHDNPDTIIFRKNSELTIPHTCLLINELKNENSKREIKHATFYPQ
                     LQDRLILLTDGITQAGMGTDKYPFGWERNNVVDFVSKTLESEPLISAQKLAQRIVNIA
                     YKLDNYNPQDDMTCGVIYFRQPRKVLIVTGPPFDEKDDKKMAQIFQDFKGDKVICGAT
                     TADIISRELNKKIIDTFEFEDPELPPISYMEGANFITEGILTLSKVSDILFHQNPPFN
                     IPRGPAGKLLQLLIDSDEIYFLVGTKVNEAHQDPHLPVDLEIRRTVVRRIARQLEEKL
                     LKEVIIEFI"
     gene            complement(42144..43973)
                     /locus_tag="PKI65_01335"
     CDS             complement(42144..43973)
                     /locus_tag="PKI65_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOB77115.1"
                     /translation="MKAQIQIDTSKCNMCYACIKVCPVHAIRSSVEETHAELIADRCI
                     NCGSCYTHCPQEAITYRNSIEKVISWINDEEKLAAILDPAISGEFIDVNDYKKFISMI
                     RQSGFDYVIDGAFAADLLAIQYKKFLDENKGKYYLFTLCPIIQNYVQKYAIKALNNLL
                     PLPILPEMSSFITKKIINEQIKTVYIGPCIAYKDISEFSNSDEVLTFEELRKLFDRFG
                     ITEIEQEFSSFDFVEGKWGNYFAIEDGILIATDQAQDILSTNVISSSGENLFKDAIFD
                     FMNYIEIIKKHFNIYYCNGCIMGPAITLTGNYIMKKYQVLQYSQKRLRDLDEEKWKLN
                     INKYINETLPVSFKNLEIKLPKVSKKQIEDILNYLQITPSAKPCKNCGFDTCEEFATA
                     IAQGIANSEMCIYYNIANKIKLIKKLNNQLEKLNQKIVAEEEKGEKLKEELQLHALII
                     ENIKELLHKVPAGILVVNGENKVEYSNEFFVKLLGDEVKEINEIVPGLRGSQIEKLLD
                     ISLVNMIEYALTHPQGETQRDILINNKWFNVSVYPIKYRSLAVMLIKDISIPEVNKAE
                     VVEEIKKAIEKELAMVQQIAFSLGEGASEVERILNKVIKIYEK"
     gene            complement(43951..44232)
                     /locus_tag="PKI65_01340"
     CDS             complement(43951..44232)
                     /locus_tag="PKI65_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOB77116.1"
                     /translation="MTEKNNDKIPIYICMGSSCFSKGARILVEIINNTIEQYQLQDKV
                     DFRGKRCFDRCADGPNIQIGDQIYNRVNPENIKSIILNYFSIHESANTD"
     gene            complement(44459..45562)
                     /locus_tag="PKI65_01345"
     CDS             complement(44459..45562)
                     /locus_tag="PKI65_01345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOB77117.1"
                     /translation="MNKNKKILIICPYPTDKAPSQRLKYEQYFNYFNDNGWQITVSPF
                     VTENFWKVIYKKGHFFEKIIYTIYGYFIRLIDLLRIKKYSIIYIHLWVTPIGLPIFEW
                     LFAKLSNKMIYDIDDMIFLGHSSDANKFIIKAKGTKKMITLMKKADHVITCTPTLDKF
                     VKQYNSMTTDISSTINTAIYVPIADYTLHNLPTLGWSGSHSTSKYLKILEPVFVELLK
                     RSIKFKVLIIGDENFRFSNTEIPFTAIPWSLDTEVNDLIKIDIGLYPLPDEPWVYGKS
                     GLKALQYMALGIPTIATAIGANFRIINDGVNGFLIPVGDIDMWIKRIIELLENKELRE
                     KIGKNARKTVEMYYSVEANKDKYQTILESLNNM"
     gene            complement(45609..46805)
                     /locus_tag="PKI65_01350"
     CDS             complement(45609..46805)
                     /locus_tag="PKI65_01350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB77118.1"
                     /translation="MNNKIKNVLYVTYDGLSDPVSQSQILPYLEKLSNNAIKFHILSF
                     EKKEKLPKLEDELWSKVEKYNWVWYPDDYTKKPAILSALYDIDRGREKIKKILKYNPE
                     ILCIHARSYISMMMAYPAAKRYNIPIIFDMRGFWADERVDGHIWSLKNPIYRIVYKYF
                     KRKEKLWLKNSDAIVVLTDAAKDFIIDKFSIASNKIYVIPCATDFDFFDPDKVEIKYD
                     NHIFNLLYFGSAENWYLFDEMFEFYNEYRRVFIPSKFTLILHSKNNKILELLNKYKWY
                     NEVEIIYNIPRASMPKYINQAMHVVFFLNQNFSNIGISPIKNAEALAMGKPIITNTSS
                     EAIVTLEEKKLGVFVKNHNKKEYRSACLKLKNTDFDSQYIRAMAREKYDLTLLSSKYI
                     DVYDAI"
     gene            complement(46802..48628)
                     /gene="asnB"
                     /locus_tag="PKI65_01355"
     CDS             complement(46802..48628)
                     /gene="asnB"
                     /locus_tag="PKI65_01355"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOB77119.1"
                     /translation="MSGIAGIYSIEANDSFLQNIYHFNEYIAHRGRDTAAYYFKSNIA
                     LGYQGSSLDINDQPLISLDGHHAIVFDGILFNFDEIKNYFFKKYPNQSIFLKTTSTAE
                     LLLELYALEGESIFAKLNGMFSMAIWDEKEKKLVILRDPIGIKPLYISHQDNYFCFAS
                     ELKAFYQILPEISVDATAITYYLHLGFIPHTHTILSQVKKLPAAHYLIIKDGKYKLKK
                     YHNFEIKKSNISYIEAKEQVRITIEKQVRGALSSDANMGIFLSGGIDSSLIASIAAKY
                     QNRPLMAFTITQDDKSLDESIYASRIADYLGLQYTPLKIDIDFLTKNFEKILDNMDEP
                     LADSSFLVTYAISQFASKKVEAVLSGDGGDELFLGYGMYRWAKRLNNPFIKSTKPLIA
                     FAFKNIPIQRFRRWSQFFEIDNYVNSNIFSIENNYFTNWQLKELLIISNDLQLPFSTL
                     DNKDPILQQAWFDFNYCLPDDLLVKIDRAGMANALEIRPPLLSAELADLAWSIPTNYK
                     ICNHELKFMLKDILADYLPRELFDRPKKGFAIPITEWCMNDLAPYIKSSLCNPKSNIF
                     NFVNYQFVNKLLINFYEKNILYLSGRIWNLFVLNYYLEKHNL"
     gene            complement(48632..49789)
                     /locus_tag="PKI65_01360"
     CDS             complement(48632..49789)
                     /locus_tag="PKI65_01360"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOB77120.1"
                     /translation="MQIIKTKKLPIYIWATEVEDGAMQQALHLAEHPFAEHHICLLPD
                     VHEGFGMPIGAVLATKEVIIPNAVGVDIGCGMCAIKTNIKYDNINRKILEKIVIQIKE
                     AIPLGFKHHKKPQPMPQHLKTFPGIITQSEKNEIPYQLGTLGGGNHFIEIQRDADNFL
                     WIMLHSGSRNIGKKVADYYNNKAKEWNKKWNNIVPDSWNLAFLHIDSEWGQQYIAEMN
                     YCLQFAKENRLYMLDTIKMIFYDYLKCDFGDIISIHHNYANLEQHFGEQLWIHRKGAI
                     SARDGELGIVPGSQGSHSYIVRGQGNPMSFMSSSHGAGRVMSRRAAVKKLNLDEVKKN
                     LDEKGIIHSVYKPRDLDEAPQCYKDIDEVMEQQKDLTKPIYKLYPLAVVKG"
     gene            complement(49938..50480)
                     /locus_tag="PKI65_01365"
     CDS             complement(49938..50480)
                     /locus_tag="PKI65_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77121.1"
                     /translation="MNQGILIILLILIPSLIVFLTALYVLHSMNERHLKEIRLLRNDD
                     IKNTFLPLKIQAYERLALFLERIAPEQLLAKMSKEDAENLTTLHQYILDTIRQEYEYN
                     LSQQIYVSRKAWLSVISAKEEIIQIINQAFNEAHSSYSEFAVKIFELYLKKDVPAIKK
                     SLDLLRSELNREVFYLESEK"
     gene            50616..51332
                     /locus_tag="PKI65_01370"
     CDS             50616..51332
                     /locus_tag="PKI65_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOB77122.1"
                     /translation="MSGILYLIPSPIGSIKEVDYLTQSNTEILQNLKYFIVESEKTAR
                     AFLHYCGHNLDDNLNFIEYNEHNFNKINIDDWLKILKSNNSIGLISEAGTPCIADPGS
                     EIVLRAHLNDIEVYPFAIPSSITMALMSSGLNGQKFSFHGYLDKNVNKLRQQIKILER
                     ESRLSGYSQIFMEVPYKSQKIFDLLLNTLKSDTFLCVAAELNTSEQFIKTKQIKSWKN
                     FQVELNKKRCIFIFQNKNYF"
     gene            complement(51512..52492)
                     /locus_tag="PKI65_01375"
     CDS             complement(51512..52492)
                     /locus_tag="PKI65_01375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOB77123.1"
                     /translation="MKILVVRFSSIGDILLTTPMIRCLHQRYDDIEIHLLTKAIYKDI
                     YLYNPYIDKLWTINNSPKEIFYNLLNEKFDFLLDLQKNFRSLWLKANLPVEFSSFPKK
                     NLKKLKMVITKKRKPISNVALRYFESAKYLDVYYDGNGLDFFFPENFIFNYELPPIYI
                     AFAISGTYYTKQYPTEYVIEYCKKSDYPVVLLGGKNEEVKGNQIKDEVGNKVFNYCGK
                     LSLIESAFIIKNSILVISNDTGMMHLAVALHKSMISIWGNTVPEFGMYPLYPDNVKIL
                     HKYFQDDSVSCRPCSKLGHDKCPKKHFACMKNLLPDKLLDISRDMIKQKN"
     gene            complement(52664..53662)
                     /gene="floA"
                     /locus_tag="PKI65_01380"
     CDS             complement(52664..53662)
                     /gene="floA"
                     /locus_tag="PKI65_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317420.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOB77124.1"
                     /translation="MLTLNLIVLNTGTNIIIGIVIFILLWLIFYFVPIGLWFTALLSG
                     VRINLLQLILMRWRKVPPKVIVNCLITATKAGINLTRDQLEAHYLAGGRVNSVVKALI
                     SADKANIPLDFKSAAAIDLAGRDVFEAVQMSVNPKVITTPAIAAVAKDGIQLIVTARV
                     TVRANIKQLVGGAGEETIIARVGEGIVTAIGSAENHKAVLENPDVISRTVLTRGLDAG
                     TAFEILSIDIADIDVGKNIGAQLQIDQAQADKNIAQAKAEERRAMAVALEQEMKAKAQ
                     EARAKVIEAEAQIPLAIAEAFRMGNLGVMDYYRMQNIKADTEMRENIVKPSSHTED"
     gene            complement(53666..54160)
                     /locus_tag="PKI65_01385"
     CDS             complement(53666..54160)
                     /locus_tag="PKI65_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOB77125.1"
                     /translation="MQIILLLDREMNLTTIILLIIAGLILLLLELVIPGGVVGTIGGI
                     ATIAGIILMYDIYGNLYGTISLVVSLIVCGVGLYFFFREKTWQRISLNDTIDKKLNKE
                     EIETLKPGMRGHTITKCIPAGKARFGDKIVEVHSTSEMIYPDTEVEIIRIDGNKIIVK
                     PFKK"
     gene            54635..55060
                     /locus_tag="PKI65_01390"
     CDS             54635..55060
                     /locus_tag="PKI65_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77126.1"
                     /translation="MDKIKYLWEYDIKVLLALEQAIAGEKEFLDFLLTNGYPELGALA
                     NAIRADLEAYQWLIRYGFLELAMLSDAIDGDGAAYDWLNNQDLYLYMIIASACQGSEK
                     AKNYLAKNNLDYFIRFADTVANELEWQRRNQTDYHKFGS"
     gene            complement(55154..55954)
                     /locus_tag="PKI65_01395"
     CDS             complement(55154..55954)
                     /locus_tag="PKI65_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOB77127.1"
                     /translation="MTKDEALNILSLSNNANENDIKKRYKELAKLYHPDSNTPESSAE
                     KFILITEAYLFLLNKNTLITNSTSNQTTQQYEDIYREQAKAYARMKYEEFKKKEEEIH
                     NISINKILYPSWLNIIFLILTVFFLFDYYLPRQWTKCQDAYIIEPISDEEDFIIKACN
                     HKILAERLSLNDIYLLEKNGVIYLNRTRLLRIICSYSINPQRRVYNVTNNRYITYPTL
                     WLSLLSSLSVLLIPFRNFQQKLFAKFLSLVFILSFLLPFIAISFSPIL"
     gene            complement(56096..57178)
                     /locus_tag="PKI65_01400"
     CDS             complement(56096..57178)
                     /locus_tag="PKI65_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOB77128.1"
                     /translation="MNKNVYCVIMSGGTGVRFWPMSRESKPKQFLDILHTGQTLIQTT
                     YNRYSKVIDKKNIRFVLSSYHVDLLKEQIQEISDEQIIVEPDRRNTAPAIAYASAKIF
                     KKDPDAILVFAPADHLIDDEDAFAETLNQAIDYVKQHDVLMTLGIKPTRPDTGFGYIQ
                     FIPQKGQKICKVKTFTEKPALELARTFIRSGDFLWNAGIFVWKAKTIIDAFKQHEPDI
                     YNAFLKDLSWYYTEKEPKQIEKIYSSIKKISVDYAIMEKANNVYTIISEFPWNDLGTW
                     ASLHEKRPKDENNNSIIGKNVMTYDTQDCLIQMPNNKKLVVIEGLKNFIVVENDDMLL
                     ILNKDHEQSLRNIVQDILIEKGSEYI"
     gene            complement(57557..57739)
                     /locus_tag="PKI65_01405"
     CDS             complement(57557..57739)
                     /locus_tag="PKI65_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77129.1"
                     /translation="MRRFRGVATKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
BASE COUNT        20866 a         8543 c         8327 g        20288 t
ORIGIN      
        1 atgattattt ctacagaatt aataaatgat gggaaagaca aagaaattaa tttatccaaa
       61 agcaaggcaa tagtattttc ttcattaaaa agtggaatta taatagagag attcataaaa
      121 aatatttttt aaaaaaaatt tttttcaaaa atatgaaaaa aataaatatt gtatctttca
      181 ttattgagtt ttgtagtcta aaaactatta agatagctat ttgtatatct atttttatag
      241 aatattttat atattttaat agatttttgc aattcagtaa aagcaggata cttagattct
      301 ttgttacata aaagatgatt tcgatatatt tatcatgtat ttaagttatg attgattaca
      361 tttgtaataa atttatatct aaaaaaatta aataaattat taaaacttat taaatcaaaa
      421 atagctgatg tgaaaacatc agctattttt tgatttaatt ttatatatct tattagctgg
      481 tagcagcttc aagttttttc attaatgcaa aaatcaacaa acctgatgcc acacaaacac
      541 ctacaaatat taaccacaat tgccatagtt ctattctttc ccagaaataa gtacctaaga
      601 ataaaagttt attaccaact gcagttgtca ataaccaacc accttgcatt aaaccttgaa
      661 atctttgagg agcaacttta gacacaaaag ataatcccat agggctcaga aataattcag
      721 caatagttaa cattagatat gtagaaatca accagtatgg cgttactctt tcataatctg
      781 taatggatga ttgagctatt tcactaggag aagttaaatt ggcagaagca aatatcataa
      841 tagtatatgc gaaagcagct ataaacatgc caatacctat ttttttaggc gtagatggtt
      901 cttttccttt atttctaagc cagctaaaaa gaccattaat tggaaaagta agagctacaa
      961 tgaatagagg attaaaagat tggaaaagtt cgggagcgat agggatcatt ttacctacat
     1021 ttatctgata ataataatag gttaaataac caaagcctaa aaagaacgcg gctgcaataa
     1081 ttttattaac taatttttta gaagtaaata tcatcacaat accagttatt gctaatatta
     1141 ttgataataa agattttaaa ttaaaaaata atgcagtaaa ggcgttaacc tctttgaaga
     1201 catagtccct agcaaagagt gtcagagtta atccattttg gtgaaaagac atccagaaaa
     1261 atataacaac aaagaataca aaaaccaaag aagttatttt aggtttctct tcaggagtag
     1321 aagataatat tatataagta ataaaaccac agaataaagc aacagcaaat cctatgtcta
     1381 catttttcat tataaaccaa aagataccta aaactgctac gaatactact attgcataaa
     1441 ttaaattttt gattggaaat ttttcagtag tgggttgttc tttatgttct ttagcttctt
     1501 gttctttctg tgctttctgt atttttcttg catcaggtaa gcttttttga tagaaaacaa
     1561 aaattaaaag agaaataatc atagctatag cagcaatacc aaaagcatag ttataacctc
     1621 ttgagaagac atcgatatat tgatgcgcaa aagcagaaag atcagtaacc acagtactag
     1681 aaacgctatt agctaaatct tgaaatttag caatatccag actttgtcca tcaaggtatg
     1741 catgacacat tgcaggcaat gatgcatcgt gaataaagcc atttattttt aagaaccaat
     1801 ttctaatacc agtagctgca aaaggagcaa aaaaagcacc aatattgata cccatataaa
     1861 acaataaata accagtatct cgcagtggtt catacttagg atcatcatag agctgtccta
     1921 cgatggcttg taaattacct ttaaaaagtc cgttgcctaa agcgattacc attaaaccag
     1981 taatagcaat agctaaagta gtacctggta cggccatcat cacataacct aataacataa
     2041 caatgaggcc caatagaaca actaatttgt aacgttttgt tgtgtctgca ataataccac
     2101 caactaacgc aagagcataa atagcgaaat aaaaccaact gtaaatactg ccagcacttt
     2161 cagccgacat accaaatttg gcttgtaaaa ataaaactaa aatggccatc atagtataga
     2221 agccaaatcg ctctcccata ttagagaaaa aagcgattac taaaccttta ggatgatttt
     2281 taaacataca tttatttttt tttatttatt tgtttctaaa taaggatttt ccattttaac
     2341 tacctgagcc atcatatttg cctccattac aatcttttcc cctacataag ctttgcctat
     2401 catgtttaca attcctcgtc ttatcgggga ggctagctct aggtaaaaaa caactgtatc
     2461 tccaggtact actttatgtc taaatttcac atctttaatc gacaaaaagt atgttatgta
     2521 attttcagga tcaggcaaat cagataatat catcatacca ccagtctgag ccatagcttc
     2581 gattaataag actcccggca tcactgcttg ttttgggaaa tgacccaaaa aatgtgtctc
     2641 attataagta acatttttga tgccaataat ataatttttg cctttttcta taattttatc
     2701 aactagaaga aaagggtaac gatgtggcat ataatgctta atagcttcta tatcataaag
     2761 aggctcctta ttataaatgt caaagtttga tacaggagat tttttagcct ctttttttaa
     2821 ttgttctcgt attattttag caaactccac atttgattta tgtccaggtt tattagctat
     2881 tacatggcca ttaaacctat aacctaccaa tgctaaatcg ccaacaacat ctagtaattt
     2941 atgcctggcg ggctcattag aataataata atctgtatta tttaaaatac ctttatctat
     3001 aattgtaaca ttatggatat taaaataatc tttataaaaa tctaattttt cttgtggtat
     3061 ttgtttatca actattacta acccattatt tagatctcca ccttttatta atccatgttg
     3121 gatgagttcg tctaattctg ataaaaaaat gaaagttcta gcatgagaaa tttctttttc
     3181 aaattcattc atatcattta aagtagcgta ttggggaggt aaaatatctt tattataatc
     3241 tatcattaca gtcaatctat aattatcatc aggtgttagc cagtattcag cacttttttc
     3301 ttcatcataa taagataatg tggtggagat gttataaatt tttctatttt cattttgttc
     3361 aataatctca gattgatttt tgatagcatt tataaatcca taagaatttc cttctagtat
     3421 aggtacttct tcattatcca tttctataat aatattatca atatctagtg ctataatagc
     3481 agctaataga tgttcgacag tgcgtattac aactccatca cgtgacagat ttgttcccct
     3541 aatagtatcc gaagcataat cagctaatgc aggaatagta ttattcatat caatatctat
     3601 tcttttaaaa acaattccag tgttttcatc gttaggtttt agagtcaatt ttacattttt
     3661 ccctatatgt acacctttgc cctcaataaa aacctcattt tttagtgtct tttgttttat
     3721 cattttttat taagtttgat attaatttat ccaaattata aactttattt cttatttcag
     3781 ctaattcttt aaaagcaata aaatttcttt taaatgcctt agcatctata gcaggcgatc
     3841 ccattagcat ctcattatca ctcacgttac tagctatacc agattgagct gctattttat
     3901 tattatttct gatactaata tgtccagaaa tacctacttg accaccaaac atattattat
     3961 ctcctatttt agtggaccca gctataccac attgagcggc cattgcatta tttttgccta
     4021 caactacatt atgagcaata tgtatgagat tgtctaattt agtaccctct tttatacgag
     4081 tagatcctaa agtagacctg tcaatggata cattggctcc tatctctacg ttgttttcta
     4141 ttatcacatt acctatttga ggaatttttt cataagtacc atcttctttt gggaaaaatc
     4201 caaaaccatc agagccaatt atggctcctg aatgtattat acagttatca cccacctcac
     4261 aatcttctaa tattactact tgtggataga tataacagtt ttttcctata tgcacatttt
     4321 cgccaatgta tacttgctct ctcaatatcg tattatcact aatgaccgaa tttttacaaa
     4381 caacactata atgacctata gaaacattat ttccaatagt cgcttcttca tctatatatg
     4441 caaattttga tatatcagta taattttttc tctttttttt agcttcatca attatattta
     4501 atatttttag aaaagataaa tatggattat taactcttat caaagtaaca ttaacaggat
     4561 ctattaaggc taaatctttt gaaattataa gtgcactggc ttgtgtatca taaatacatt
     4621 tttcatactt aggatcaaaa ataaaagcaa tatcaccttt tttagcctct tcgattcttg
     4681 ctacatttct tatctctatt gtatcatccc cttctatttc gccttctaaa agctgggata
     4741 tttgactaat tgtaaacttc atatattaaa aaaaaaatta attaaaaaat taataaagct
     4801 tattttgtaa ttctggagtt gctatgggtt taatagcaaa attaaaaatt gcaaatatta
     4861 aaaatgtgaa aaacaatgtg cctataattt catacctaaa aaatggaatc gccataatat
     4921 aacaatttat taacccatgc cagctgtatc cataccaccc ccccaaccac acgccaaaat
     4981 tggataatac aaaaaatgta aaagaagcag ttattgaaga taaaaatact gatgttacct
     5041 ttatcttttt gatcatttta gcacctatta atgctatcac aacaaaactc aaataggtaa
     5101 aaatcataac tatgctctga tataaaccta taaaaagatc actaataaat agagcaataa
     5161 caggtaatat gaggtaccac tttttatttt taaaaacata accacataat aatgctagtg
     5221 ctgcaatagg tgtgaaattt ggaataaaat taaaaagtct atataccact actaataata
     5281 taagaagtgc taataacaaa aaatgtaaaa aatcattttt tttcatagtt atttttttgc
     5341 aaacttataa aaaaaatttc atttaaaaaa cgagaaaaat tttttgttgt taaaaaataa
     5401 aataaaaatt agaaaaaact aaataatata tcaatgttta cagcaatcat tttattatat
     5461 attaattatt tataatatga ggcataagac aaagtatgat tattaggttt cgaaaaaaag
     5521 cttcctgcaa attcatcttt gttttacttt acgtagttta tactaccatc aaataatttt
     5581 tatttaattt tacaaaaaaa taattatgga taatttcaat ctattagatt atcaagctct
     5641 acccaaagag cagaaacgta gattcctaga taatctatat caatttttat tagaaaacaa
     5701 ctacacagcc gtagattatc agaagctaaa aatacaaagc acagccccca tctgtcctag
     5761 atgcgaaagc gaaaatgtaa taaaagctgg agtacgcgac ggcaaacaag tgtacaaatg
     5821 caaagattgc ggccgacaat accgcgaaac agctagaacc ttcgtatatc gcatgcgaaa
     5881 agctgataaa atgctcgatt acctaaaatg catgttagaa ggcaaaagtc taagagcttg
     5941 cgcatcggaa gtaggcataa gtctgagaac gagtttcaat tggcgacaca agatactagc
     6001 agcgatcaaa tcattacagg gtggtatcag cttctcaggc attgtagaag ctgatgagct
     6061 gctaatgaaa tattcagaaa aaggtcgcaa atacagaagc cgaaaagaat acgaaaaagc
     6121 aaagaaaaag aaacatccca aagtagctgt gctagtaatg acagacaggg aaggcaattt
     6181 acttttcaaa cacgtaggac gcaaaaaagt aaccaatagt cagataagag aagagctatc
     6241 gcatagagtg acagatagca acttaatatg tgtgaagcaa aagaaggaat tcaaaaaagc
     6301 tgtgaagggg accaaaagta aaaaagttgt tgtaaacaaa acgcagatta gacgcggtgt
     6361 atacagtgta caggttgtcg ataagcatat aaatgatttc aaggtttggt tgcgacgatt
     6421 tagaggagtg gctaccaaat acttacagag ctatctgatg tggtttgtaa tcacacataa
     6481 atacttgaca agtgagattg aatcagacat tggtcggctt ttaaatcttg catcctcgga
     6541 cagatgggcg tgggatgagt attggcggct agtgaatacg aaatatagat aaactacttc
     6601 aagtaaaaca atgatttgtt tctagaccct cttcgatttc tttcacaatt acaataattt
     6661 tcctttctat aatctctatt ccaattaaaa taaatagagc caagaaattt agctttgtaa
     6721 aaattgcgct tcgtaaacgt gatttgtgaa cggagctact taaaaatatc ctttgtttta
     6781 cttaaagtag tttatagcat aattaaataa ttagacgcgg tgtatacagt gtacaggtag
     6841 tcgataagca tgtaaatgat ttcaaggttt ggttgcggcg atttagaggt gtggctacca
     6901 aatacctcca gagctatttg atgtggtttg taatcacaca taaatacttg acaagtgaga
     6961 tagaatctga cattggtcgg cttttaaacc tcgcatcctc ggacagatgg gcgtgggatg
     7021 agtattggcg gctggtgaat acgaagtata gataaactac ttgaagtaaa acaatgattt
     7081 gtttctagac ccctttcgat tcccttttga ttctaccaag taatatttta gctagctaaa
     7141 atattatcta acctttacta tacttctttt tacaactcta ttaaaattac atattgctag
     7201 agggaaatat gaaaaattat aattctcatt atttatccaa taatttattt tttaacaaac
     7261 acgcatcgcc ataactaaaa agcctaaaat cattttctaa agcataatca taaatatttc
     7321 tccatgaaat agaagaaaaa gcgtctacaa gcattaataa agtactttta ggctgatgaa
     7381 agttagtaat caaataatct gctgataaga ctgtagctgg tggcaatata aataagcttg
     7441 tatgtccata taaattatca aaatttttat tttctaagaa tttttttaat ttttcaatta
     7501 atatttttct atcacaaatt ttattatatt tcctatgcat aatatcaaat tggttaatat
     7561 ttataggata agggtacaaa ttctcggcaa ataaaagtag actttctaaa caacgcaatg
     7621 tagttgtacc aacaacaacc caaggtctat taatattttc atacaaaaca tctatagtgc
     7681 ttttgttcac taaaaactct tctctatgca tttgatgttc ttcaatggaa gttttaacag
     7741 gtttaaaagt acctgcgcct acatgtaaag tgacaaaaat tttttgataa cctttgtttt
     7801 caaatttttc aaaaactcta tctgtaaaat gcaaacttgc tgttggcgag gcaatagaac
     7861 catctacttt agcaaaaact ggttgataaa aaatcttatc atcttctact ggtttacgtt
     7921 ttatataatg tggtattggc atctctccga ttaattctaa aatttctgat aaagaaaaac
     7981 cttcaggttg ccatttaaaa gttactatat tttcattgtt ttcgagccat tctacatcta
     8041 tttgtatata tatattttga ttgtttttag taaaatagta agataaatgt ggtgaatttc
     8101 taagtttttt tgcatttcct atcatacatt tccatttaga catacctata tcttggagtg
     8161 cttgtgctat gctatctgca tttacaggtt ccatcaaaag aaattctatc catgcgcccg
     8221 tttctttttt taccataatt cttgcaggta ctactttagt attatttaaa attaatagtc
     8281 cattttcagg taaataattt ataatattat aaaaataatc aaattgaaga ttttcattgt
     8341 ttcctattaa tattttggag ctatctcgag gttccacagg aaattttgct atcttttcat
     8401 ttgaaaattt ataatcataa tcatctaatt ctggtaattt aatttctgtc atcgttgata
     8461 aaatttaatg caaaattaaa actaaaagta ataaaaatga taataaattt gaattttttg
     8521 ttttgttttt ataattcaaa atttgtatct ttgctaaaat ttatttttaa aaaattaaat
     8581 tatgaaaacc aaaatttttc caatttttat gatagtaatt ttttttataa atagtggatg
     8641 caaaaagaaa actgaaactg tagaagcaat acttcccatc gactctttag ctatttatgc
     8701 tacttatgaa ttaaagacag atctatcaca cctaacagat gcagaaaagc aagccataaa
     8761 attattgata aaagctgctg atattatgga tgagctattt tggcatcagg catttggtga
     8821 taaaaatttg atggatacca tatcaaatga tactcttaga cagtactcat atataaatta
     8881 tggtccttgg gataggttaa ataataataa gccatttatc tctggttatg gacctaaacc
     8941 tgatggggct aatttttatc cagcagatct gacaaaagaa gaatttgaaa attggcaaaa
     9001 tactgataaa acttcgctat atactatgat cagacgagat gcaaatggta acttactagc
     9061 tatttattac catgaatatt ttaaagaaca actaagtaaa gctgctgatc tgatagagca
     9121 agctgccaaa ctaataaatg ataagaattt tgctaattac ctctcactac gttctgaagc
     9181 tttgaaaaca tcaaactact acgcatctga catggcatgg ttagatagta aaacttcaaa
     9241 aatcgaatat attgtggggc caatagaaaa ttatgaagat cggctctttg gttacaaagc
     9301 agcttttgaa tcttttgtat taataaaaga tatagaatgg agtaaaaaat tagaaaaata
     9361 tggcaaatta ttgccacaat tacaaaaatc attacctgta aatgatgaat ataaaaaaga
     9421 aatacctgga tctatgggcg atataaatgt atatgatgct atttattatg ctggagattg
     9481 caatgcaggt agtaaaacta ttgctatcaa tttacctaat gacgaagagg tacaactaaa
     9541 aaaaggcact cgtaagcttc aattaaaaaa tgtaatgcaa gcaaaatttg acaaaattgt
     9601 tgtgcccatt gcagagttaa tcttagacga aaacatagct ggtaatgttg tatttaatgc
     9661 tttttttcaa aatgttatgt ttcacgaggt agctcatggt ttaggtataa aaaatactat
     9721 caataataaa ggtactgtta gagaagcact caaagaagct tatagcccta tagaagaagc
     9781 caaagcagat ataatggggt tgtatctaat agaaaaatta agagaaatgg gggaaataac
     9841 tgaaggtcta ttagagcaaa attatgcatc ctttgtagct agtatctttc gttcggttag
     9901 attcggcgcc gctagtgctc acggtatagc taatatgtta gaatttaatt ttttggtaga
     9961 tgctgctgct atttctaaaa atagtcaagg taaatataat attgacttcg aaaaaatgaa
    10021 aaatgcgatt tctgatatgg tccaaaaaat tataactatt caagctaatg gtgattatga
    10081 aagtgctata cagtggatta atgaaaaagg aaaaataaat acagatttac aaaaagatct
    10141 aaataaaata aatgatgcta aaatacccgt agatatagtt tttaagcaag gtattgatgt
    10201 tttaggtcta taataaactg attagagtta tcatttgcaa ttttacataa aaacatacca
    10261 taatcttttt ctattctatt tacttcttat aatacattaa ttataaaagg cttttatctc
    10321 atttcgtaaa aaaatgttat taatatttta tttttttcgt ttaaatatca aaaaaatcat
    10381 ttaatttcgt tcagatattt tttatattat gattaatatt agaccaaact ataatataat
    10441 gccactaaaa gaattagagc aatacatttt acaaaacaaa catctacctg atgtacccaa
    10501 tcaagatgaa atctcaaaaa atggtatgta tgtgtatgag atgaatactg tattgctaaa
    10561 aaatgtagag gagcttacac tgtatgttat tgaattggag aaaagaatag atgaaatgga
    10621 aaaagtaaaa taaaggagtg ttttatgaaa caaattattt tatcatttat tttaattata
    10681 tacagtatta atacttttcc acaatatatt aattataatc ttacaaatca atctgaaact
    10741 ttattatcaa gcaataatga attaaattta tcaaaaatga atgacacaag tgctttttat
    10801 aaatctgaac ttggttttac tactggattt aactggtttt cattattcac acctaaaaaa
    10861 gaaaacaaag aatatggtga tttagattac ctttactatt atgataatta tattgcttct
    10921 gttttttata taagaaatta taatcaaata ttatcactag gattagaaat agctaatact
    10981 gctactaaat taaaaacaaa aagtgctgat gtaataggtg ctatgggaca taaatatcaa
    11041 gatttagatt ataatataaa ttatttgaat tttatacttt tattgcagtt taatatattt
    11101 tcaaaaaaca aaattgatat taatattaat ctaaatccat acataagtta tatcatatat
    11161 tctaaagtaa caggatatat tgagagttgg tctacaatta ttcttcagga tagtcttggc
    11221 aatttatttt caagaccctc acattctatt attaatttaa atgaaagtcc tatcaaagat
    11281 attaataaac ttttgcgagg ttttagaata ggctatgaca ttgaatatgc atataaacct
    11341 aatgtatctt ttttatttgc taattacttt tgttatacca catctacaaa tatatcgtgt
    11401 agtaattgtg agggtaaatg gagaagtata gcattctctt taggaataat ctataaattt
    11461 aataaatcct atgaaaaaaa taatattaat taattttatt attttattaa gtctatttaa
    11521 gatttatagt caagataaaa cttttttatc tacagaattt aatacaaact acttttatta
    11581 ttttttagga gaaaataata ataagtttaa ttatggtttt tcattacaac tttctgaaaa
    11641 tatcaataaa tttaaaatta gttttggtgc catttatgct actaaatatt actgggataa
    11701 accatctgaa aaatataaca agtgtgaata tttattaaaa aatattcgtt tacaaattat
    11761 tggcaattat cagattatat caaaaaatac tttttcaatg gatattttag gtggttttga
    11821 ttttgatcaa ataatagact atgatgtgaa attatattat gtaaatggtt atagagaaaa
    11881 aataaataat ataaaaatac aaaacaaatt gggtgtatct tgtgtaatgg gacttaattt
    11941 ttcaaaatta ataagtgata gagtagcttt aaatttatct cctaaatttt atattactat
    12001 taagcaggat catgaaccat atagtccacc attaggtcct attagtgagc cattttatga
    12061 aaatttgatt gatgatttgt ttacatttaa tttatctata ggcatagaat atttatttaa
    12121 aaaatctgaa caatgatatt tgtttggcaa caagtatgtt gccaaacaaa ataatattat
    12181 agcaattcac aaattgctca ataaaaaaaa acatcgtctt gttaatacat aaatccttaa
    12241 aaaagaagtc aaaaataaat tttcctctct atcaaaaaac gtaccattaa ttttttccta
    12301 tttttttcaa tcctaataaa atatagatta ttaaagactt tgacgtcatt tcataaaaaa
    12361 aagttattga cattttgttt tcgagtggta aaaagtggta aaaagtgtgt atatttgtat
    12421 actaattaat aagtatatct attataaaaa aataaaataa tgttcataat tacaggaatg
    12481 acagaagtag cagtagatac aaaaggtaga tttctcatgc ctgctttttg gaaaactctg
    12541 tttactcaat atcaaataaa tgagtttgtc atcaagcgcc atctctatca ttcctgtcta
    12601 gagctgatac ctatttctgt atggaatgat gagctcgaga aacttaacaa acttaataga
    12661 tttaataaac aaagtaatga ttttatacgc ttctacatgg ctggggtcag agtaatagaa
    12721 tttgataaag taggtagaat acagatacct aaagatcttt atgattttgc tgatatcaaa
    12781 aatactgcgg tgctgagtag tagcatagac agattagaaa tctgggacaa agagcaatac
    12841 gaaaactatt taaataaaca ttcaaacgaa tttcaaaaat ttgctgaaga gtttcttggc
    12901 aaatcattta tgtctgacga ggaggataaa tcatgagtaa tcaaggatat catataccag
    12961 ttttactcaa agaagtaaaa aatgaagttc ccctgctcga tcgcggtata tacgtagatg
    13021 ctacttttgg tagtggtggc catagccaag ctatattgtc cattatttca aaaaaaagta
    13081 aactttttgc ttttgatata gatgtagatg ctctaactaa tgatatcaat gataaaagat
    13141 tcacactgat caatgctaat tttaaatttg ttcgctttta tcttaattat tttgaagctt
    13201 tacctgtaga ttttatctta gctgatcttg gactttcatc acatcatata gaaacagata
    13261 ttaggggctt tagctttatg tctgatgtac ctctagatat gcgtatgaat caagaacagc
    13321 aaattaacgc taaagaaata ttaaataatt tttcagaaga tgaactaata caaattttta
    13381 gaaattatgg gaatgtgcct ttcgcaaaat ctctggctca tgatatcgta aatagtagaa
    13441 aaactaaacc cataaaaaca tcatcagaat taaatgattt ggcgttaaaa tatattttaa
    13501 agggaaaaga atttaaaaat ctagctaagg tatatcaagc tctgcgtata gctgtaaata
    13561 atgaggtaga taacctgaaa atgcttatcg atgaagcata caaatcatta aatcctggtg
    13621 gcaaactttg tatcattact tttaatagtt tagaagatca gatagtaaaa aatgaattaa
    13681 gaaaaaaaat agataattta ttagatcaaa attggttaaa aggtactaaa catcaaaaat
    13741 ggtctaagat ttataaaata acccctaatg atgaagagat aaagtctaat cctagagcac
    13801 gtagtgctaa aatgtatgta gctattaaaa atgaaaattt atgagtaaaa ataaatcaaa
    13861 aaaggataaa aaagataaaa aaaatatcat caaagctttg atgagtggcg actacttagc
    13921 ttctgatcct cttatcaaac aattaccctt catcgcttgg ataacttttt tgatgatact
    13981 ctacattgct aaaaattatc aaatagataa aactctatat gaactaggta aaataaaaaa
    14041 tgagaatgaa gctctaaaaa ctaaaagtat tgtgctaaat gctcgaaaaa tagcaactat
    14101 gaatctagaa tattgttata aagaattagc aaaagatgga cttaaaccct ctgatcaacc
    14161 acctatcaaa atttttgatt attcaataga aataaataat aaaaaaataa aaaaaagtag
    14221 aaaaaatgcc tcaagataaa atcccatttg atgaaatagc taaatccaaa atttttggaa
    14281 gagctagtgt agtctttttt attgtcgtat ttattggcat tttgattatt tacaaaatta
    14341 ttgatattca atttgttaga ggcgatgagc tacgttctca cacctttgat tatgaatata
    14401 aatatgtaga aatcactggc atcagaggca atatatattc agaagatggt atggtcttag
    14461 ccacttctat acctgtttat gatgtttatt tagatctaaa cgaaaaaaat gtttcaaaag
    14521 acttatttga tacacagata gattctttat ctctaaagtt agcaaataaa ttcagagaca
    14581 aaacagctaa cgattacaaa agttcattaa ttaaaatcag aaagtctggc aaaaggtatg
    14641 taaaattcaa atcaaattta tcttatgagg atgtagagga gctaaagact tttcctattt
    14701 tcagtcgtgg acagtatggt ggaggattga tattagtgaa gaaagataga cgagttaaac
    14761 ctcttggcag catagctgaa cgtactatag gattttcaag agacaacttc aaagtgggca
    14821 tcgaaggagc ttatgacagc attcttgctg gtaacaaagg ttatcaatgg atgcatagaa
    14881 tagctcctgg agtatggata cctgccgaag atacatcatc tactataaaa cctatagatg
    14941 gcaaacatat caaaactacc atcaatgcat attatcaaca aatagctact agagctctcg
    15001 aaaaagtttt aaaagaaaat caagctgacc acggtacagt cttattaatg gaagtagcta
    15061 ctggcaaaat aaaagctatt gctaatctaa aatgcatgcc agatagctct tataaagaag
    15121 tatataatta tgccatcggc gaacgctacg aaccaggttc tactttcaaa actattagcc
    15181 tcttagctgc tatcgatgat ggattaatag atttggatga aatcatcaat acaggtagcg
    15241 gtacgtggcg ttatggttca caggtgatga gtgactctca tgatggcgga tttggcgaga
    15301 tcagtctaag aaaatgcttc gaacttagta gtaatatcgg tatatctaaa gctatcataa
    15361 aagcttacag ctccgatgaa aaaaaatttt ttgacaaatt agaatctttc ggtatcactc
    15421 ataaaaccgg tattgacata aaaggagaac ctaatccatc tgttaatttt ccaaactcaa
    15481 aattatattc cagatatacg ttggcttgga catctatagg atatgaagtc ttattgacac
    15541 ctctacaaat attaaccttt tataatgcaa tagcaaataa tggaaaaatg atgcaacctt
    15601 ttctagtaga tgaatttatc gatgaaaata gaactatatg gaaaaaagaa cccaaagtat
    15661 taaataaaaa aatcgctaaa gatgcatcta ttaaaactat gcaatcttta ttagaaggcg
    15721 tcgtacagca tggcacagca tctaatatca aagattctat tattcccatt gcaggcaaaa
    15781 ctggcaccgc attgatagct aaagatgggg gatataaaag tgaaggcaaa gaatatgtag
    15841 cttcttttgc tggatatttt cctgctaata atccactcta ttcctgcatt gttgtagtaa
    15901 ataaacctaa acaaggatta tattatggtt cacaaatagc tgctcctgtt ttttatgaaa
    15961 ttgctaaaaa aatttattct actcaactag aaatttatag tatagaaaac aaagaaactt
    16021 ttaataataa tctttacttt tttactcctg ctcaattaat caaaaacgta caatctaata
    16081 ttaaagtaac tacatatcct ttttatacta atgatggttt tgctcgcctc gatactaata
    16141 aaatagatgt aatagcaacc tctcatttca ttaataatca aaaactacct gattttaaag
    16201 gtatgctact gcatgatgca ctttttataa taaaaaaatt aggattaaag gtagaaataa
    16261 aaggtgcagg tagagtaata gatcaaaaac cagcaccgtt aacaaatatt gataaaaatc
    16321 tagataaaat agtcttgttt ttgaaataat acaaaattta tgaaaaattt aaaagatatt
    16381 ttacatagca tagaagtaga aagcataaaa ggcgatatta atttaaatat cgacaacctc
    16441 agcaataata gcaacaccat caaggcaaat ggattgttca ttgctaaaaa agggtataaa
    16501 gtagatggac atgattttgt aaataatgca ataaataatg gtgctgtagc ggtagtgtgt
    16561 agtgaaaata ttcctattcc agaaaatatt accaaaattt tagtaaaaga ttctactgac
    16621 gtagagggta aaatagccga taatttttat gatcaccctt caaaaaagtt aaaagttatc
    16681 ggtatcactg gcactaatgg caaaactact actgctacac tgatttatca attacttaat
    16741 aatcttggca aaaaagctgg cctcatctct accattactt ataaatatgg taatgtagag
    16801 ctcccatcta taaatactac tccagatgcc atagaattaa atagattact aaatgaaatg
    16861 tggagaaatg gttgtaaata tgctataatg gaagtaagct cacactctat tgttcaaaaa
    16921 agaatcttgg gaataaattt ctctggagct atctttacta atattactca tgaacattta
    16981 gattaccacg gtacttttga aaattatata aaagctaaaa aaactttctt tgataattta
    17041 cctgccgatg catttgcgct aataaacatc gacaataaaa atagtaaaat aatggttcaa
    17101 aatactaaag ctcaggtata tacatacggt ttagcaaaga tggcagattt tcacgctcgc
    17161 attttagaaa atactttaga aggtttacac ttagaaatcg atggttatga tacttggttt
    17221 ctattggcag gcaaatttaa tgcatacaat atactagctg cttatggttg tactaaaata
    17281 ttaggctacg acaaacaaga tattttgtca gagctttcta aggtagaacc tattaatggc
    17341 agatttaatg taattaataa tgatggagta tacataatca tagattatgc tcatacacca
    17401 gatgcactag aaaatatatt aaatacatta aatgaaatag atattgatgg caaaataatt
    17461 acagtattcg gagctggtgg caatagagac aaaactaaaa gacctcttat gggcaagtta
    17521 gctgctgagt atagtgatat tgtcattatc acttctgata atccgcgtaa tgaggatcca
    17581 gaagctataa tctctgatat agaaaaaggt gtaccgacta atttaaactc aaaagtttat
    17641 aaaataactg atagagctga tgctattaaa atggctatca atttagctaa caaaggtgat
    17701 gtagtagtga tagcaggcaa aggccacgaa acttatcaag aagtaaaagg cataaaacat
    17761 cattttgatg ataaagagat tgtattccaa atcattaatt caaaaaaaca atgaataaga
    17821 aaaattaaaa aaaatgttat attatttgtt tacttattta gatagaattt acgatttccc
    17881 aggtgctgga ttatttaatt atatatcatt cagagccgct atgtctgctt tattttcatt
    17941 attaattatt ttgatctttg gtaaaccttt tataaattat ttaaagaaaa agcaaatagg
    18001 agaagaaata agacacctgg gcttagaaga agaggatcgt aaaaaaggga cacccacaat
    18061 gggtggaatt ttaatttttt tagcaataat aatccccgtt ttattatttg caaaattaga
    18121 aaatgtatat gtgatattga tgctgatcac tactataatg atggctggat taggctttat
    18181 agatgattat attaaagtat ttaagaaaaa caaaaaagga gttagtgaag gagccaaaat
    18241 cattgtacag cttgctctag gtctcatagt agctttagtg atgtataata ataagcatgt
    18301 agctatcaga gagcaaatat cacaaactaa tattgtagcc gatgtacaag aagccccggt
    18361 tatgcgaaca gcaagtgatt atttcggtcc cgagcaaata ggttcactca aaactactat
    18421 cccttttgtg aaaaacaatc aatttgatta tgctaattta gtgaaatgga taggagaaga
    18481 ttatcaaaaa tgggcttgga tagtttttgt acctataatt ttaataatag tcttattagt
    18541 aaccaacgga gccaatctaa ctgatggatt agatggttta tgtgcaggca cttcggctgt
    18601 agtgggtgca ggattagctg tttttgcttg ggtctcaggc aattttgtct ttgctagcta
    18661 tctaaatatt ctatatctac ccaattcagg agaattgaca gtattcatag catcatttgt
    18721 tggctctcta attggcttta tgtggtacaa ctcctatcca gctaaaatat ttatggggga
    18781 tacgggcagc ttgatgatcg gcggcataat agctgtattt gctattttgg taagaaaaga
    18841 acttttgctc cctttattat gtcttgtttt tcttttagaa ataggatcat cagctataca
    18901 aagatatgtt ttcaaatata gaaaatggag atttggacta gaatatgcac aaaagaacag
    18961 agtttttttg atggcaccac ttcatcatca ttttcaaaaa aaaggttggc acgaggttac
    19021 tatcgtcaat agatttatta ttatcaatat tattgcagca atattagctg ttattactct
    19081 aaaaattaga taattatgaa aaaagttaat aataaaatga catatatact aaatgatatt
    19141 attagtaaat ctaataatat ttcattagca gaaaatatcc cacatctact agatgatcaa
    19201 ttaaaatcgt caaaaaaatt atctaaaaat tatctaaaat attttactaa aaatacaaat
    19261 agattggttg ttttaagaaa attaaacaat gttaaattca tagatgatag ctatgctatt
    19321 aataattatg cattgtgcta tgctttagaa atgactaatg atcctatcat atggatatca
    19381 ctagataata gcaatattta tttatcaaaa tatttaacag aaaacataga aaaaaaagta
    19441 aaaatctgga taaatatttc taaaaataat gacattcata atagtattta cgcttatttg
    19501 gtacctgaat tttatacagt aaaaaattta tctcaagcta tcaaattggc ttatgcattt
    19561 gcagatccag gagataatat actctttgca cctaataaat ctaatataat tagtaaaaca
    19621 tattctaata aatttaaaaa aatagtaaat gaattatgag taaaggtata tttaagattt
    19681 ttaaaggcga taaaactatt tggataatat atatattttt atgtatattt tctatgatag
    19741 cagtatatag tactattgga aatttagcat ttagattcaa tgaaggcaat acgacatatt
    19801 atttaataca acatataata atattaattt tcggtatagt attagcatat ttagtacatt
    19861 taatggacta tcgtatatgg ggctttttag caaggttttt attcttaatg gctgtaccat
    19921 tattaatagc aacattgttt aaagggcata ctactaatga tgctgctagg tggctgagaa
    19981 taccagttat aggtatttca tttcagagct ctgattttgc tagatttgca ttaataattt
    20041 tattagctcg tatagcagcc tctaaacaag ataatataaa agatttcaaa gaagcttatt
    20101 tgcccatgat catagctgtt gcgatcactg taggtttaat atttcccact aatttatcta
    20161 ctgcaatatt aattttagct acagcattgc tgataatgta tatagctagg gtaccattga
    20221 aatatttgat tgttactatt ggcattgcag tagttataat gggaatttca attttaataa
    20281 tgctacaaat gcccaataaa gggcgcgtag gcacctggca atctagaatt gaaagtttca
    20341 ctaataaaaa tggtagtgtc ccttatcaaa gaatgaaagc tgatattgcc attgctaatg
    20401 gtggtattgt aggtaaattt cctggtaatt ctactcaacg cggtactcta ccacagtctc
    20461 atacagactt tatctatgcc ataattatcg aagaattagg aatagtagga ggcattgctg
    20521 tattgctttt ttattgtgtt ttgatgtata gagctattca tgttggtaaa caaagtaaag
    20581 ggtttttcgg ggcctttttg gctataggat ttggttttta catcggcttt caagcgatag
    20641 tgaatatgtc agtcgctgtc ggccttatac ctgttacagg acaaccactt cctatggtta
    20701 gcaaaggagg tacttctatt atttttacct ctatcgcagt aggcataata cagagtattg
    20761 cccgtgattg tcaaaaagat caaaaacaaa tagacgagca aatggaaact gatgataatg
    20821 aaataattga aaatattagt gaggcaggat gattacataa aaagttataa aaaatgaaaa
    20881 taaaaaatga aataaaaaaa tgggttattt tctctgctgg agggacaggt ggacatatat
    20941 ttcctgcttt agctgtagct gagcggctta gagaactgca tccagaactt ggcatattat
    21001 ttatcggtaa taaagccaga atggaatctg agataatccc aaaatatgat tttcctataa
    21061 tattactaga tatgcatggt ttagatagaa aaaatatatt aaaaaatatt aacctgccaa
    21121 ttaaactaat aaaatcttgg ataaaattaa ataaaatttt taaaaaattt aaacctaaag
    21181 tagttattgg ctttggagga tatgtaacct atcccgtact caaattagct cagtggagag
    21241 gtatacctac cattattcaa gaacaaaata gcactcccgg ccttgctaac attaaattaa
    21301 gtaaaaaagc ttctgccatt tgtgcagctt atcctaccat aaaaaattat ataaaaactc
    21361 ctaatgtata tatgataggt aatctagtga gacaagaaat tttgcttcca atgtctaaag
    21421 aagaagctgc taatttctgg aatctagata ttaataaaaa aactatttta gctctgggtg
    21481 gtagccttgg tgctaaagct attaatgatt ttatatgtaa tattttaaca gatttgataa
    21541 gtaaagattt acaattgata tggcaaactg gcgataaata ctcaataaat agctgtttaa
    21601 atatagataa attaaatgat aatattattg taaaaccatt tattagagaa atgagagaag
    21661 cttatactat ggcagacatt gtcatagctc gtgctggtgc aggtacatta gctgaattgt
    21721 ctgctctagc taaaccatct ataattatac cttcgccaaa tgtagttaat aatcatcaat
    21781 atcttaatgc tcaagctcta gctgaaagca atgcaattat cttactagat gagaatttat
    21841 tagatataaa aggcaaagaa atattattgg atttaataaa taatgaaaac ttacaaaaaa
    21901 aattaagcaa aaacattagt aaatttcata atccaaatgc aataaatgat tttatagaat
    21961 taattgaaaa atatttaaaa aattgataaa aatgaaatta aataatatag aagtcaaaca
    22021 tatatatatg ctaggtatag gaggcatagg tatgagttca ctggctcaat attttctcta
    22081 taatggttgc aaagtgtctg gatatgatag ggtacgttca gagatttgta atattttaga
    22141 aaataaaggt gccaatattc actatgaagc taataatgat ctagtgccta atgatataaa
    22201 tcttgttgtt tatacaccgg caatagctga tgattttact gaatgggaaa ttataaaaaa
    22261 taagaaatta cctgtttaca aaagaagtga tatattagct atgatcacac cacagtttaa
    22321 aacctttgct gtagctggca ctcacggcaa atctactgta actgccatac tatctcatat
    22381 ttggaatact gctgatagag actttatgtc tatggtgggt gctattatca aaaattatga
    22441 aaataatttt attatcaatg gtgaacctaa agaatttatc gtagaagccg atgaatatga
    22501 taaaactttc ttgaaattta ccgtagatac tggtatcatt acgtcaattg aaccggatca
    22561 tttagatatt tataaaaatt atgaagattt aaaaacagca tttacagaat ttgctaataa
    22621 gacaaaaaat gaaatatggc tagacgaaaa tgtaaatttg caaactaata aaaaaaatct
    22681 aacctatggt ttatctgaca attcagatat ttttgcttat aatattaaat taaaagatag
    22741 taaatacatt tttgatgtta aatctaatta tcataatata aaaatagaaa aattacaatt
    22801 aaatattact ggcaaacata atattaaaaa tgcgattgca tctattgcta ttgctttatc
    22861 aaataatatt aatattgatc aaataaaaaa agctattagt acatatcctg gcctaaaaag
    22921 gcgtttagat ttaatttata atagtaaaaa taaaaaatat tatgatgatt atgcacatca
    22981 tcccactgaa atagatgcat tatttgaagc attgaaaagt atatatcccg acaaaactct
    23041 ttgtgctatc tttcaacctc atttatacag tagaactttg tatctagctg atgggtttgc
    23101 taatgcatta tctaaatggg atagaataat tttattagat atttatcccg ctagagaaga
    23161 acccattgat ggtgtaacat ctgaatggtt attaagcaaa attgataata aaaataaata
    23221 tttaatgaca aaggatgaat tattaaataa tatagaaaaa attaacgaag atataatagt
    23281 aacgataggt gctggagata tagatagact aagagaacct ataaaaaata aattaataga
    23341 atatgaaaca taaaaagtgg ttaatagcat gtagtattat tttaccaata attattatta
    23401 taggctggct atttattagg gacaagtcag aagccacatc tattgctcga atagatatca
    23461 atatacatac tgctgacact aataaagtaa tagatactac atatgtgtta gatttaatat
    23521 accaggttac tcctagagtg ataggaatgg tgatagagga ttttccttta gataatattt
    23581 atcaaacaat aaaaaaatca ccttttatag aaaaactttt gtgcaatatt acattagatg
    23641 gtacactcaa gatagaagct tgggcccggt gtccattatt gcatatcatt tcaaataatg
    23701 gctacgagtg catattagat acatctggat atctgatgcc tgtaccaacg agatataatt
    23761 tatatatacc tgtggcttat ggtaatatta aaacaaaatt aaaaattggc acaaaagttc
    23821 aaaatattca gtcatacact tatgaatcaa aaggcttcac tgaattagat aagctctttt
    23881 atttagcaaa aaatattaaa aacaacaaaa tatcaaattt attattttgg gatatatatg
    23941 tagaaaatcc gaaatcattt tatctatatc ctctagtagg aaatcaatat attaaattag
    24001 gagatataga tcaactaaat gaaaaactaa aaaatatcac tatattttac aaccatatac
    24061 atcagatagc caatgtagat gaatatgaag gtttcgatct gcgagtaccc ccacaagtaa
    24121 tagcaatcaa acaattatga aaaatttttt aggtattaat ttaaaaataa ataaatatga
    24181 aaaaacaagt tgacacatta gaagaacgaa tacatgtagg cttggatata ggcacaacaa
    24241 aaatagctat ggtagctggg aggcttaatg gtgatggcaa attagaaatt ttaggtcatc
    24301 aaattgttca aagtaaaggt gtacgaagag gtacagtgat taatgttcaa aaaactgtag
    24361 atggtttaaa agaattgaaa atgaaaatgc taaaggactt tggcatagag gtaggtatag
    24421 tacatctagg aatagctggc aaatacattg aaagcctaaa catagagcat agcattattc
    24481 gtaaaaatcc taacaacatg attacaggac aagaaataaa tgatttttta gaaactgtaa
    24541 agcaaatgct caagcaacct ggtagagatg ttatacacat tatcccacaa gaatacacta
    24601 tcaatgatga atctgggatc acagatccca taggagcggt aggttctaga cttagttgta
    24661 aaatacatgc agcagtagct aatatatctg ctatagaaat gctaaaaaac tgtgttagta
    24721 tggcagatat ggacattgca gacattatgc tagaaccact tgcatctgct gaagctgtat
    24781 tagaggaaca agaaaaagaa ggaggtgtag ttttggtaga cattggtggt ggtaccacag
    24841 atatagctat cataaaagaa aacatcatca gacatgtgtg tgtcataccc tttggaggtg
    24901 aagcagtaac agaagattta aaaaacggcc tttcgttatt aaaagaagag gctgaattat
    24961 gtaaagtaaa ctatggaaat gctataccag ctgacgtcaa tccaaatgaa gtaatagcct
    25021 ttcaggctat gaaaaattgc cctcaaagag aattttcact tagagctatt agtgaaatca
    25081 taagtgctag aatgtcagaa atcttagaaa ccgtaaatta tgaaattaat atttctaatg
    25141 ttacaatgcc tctaataggt ggtatagtgc tgactggtgg tggatctttg attaaaaata
    25201 cctctcaact atcttcttat attactggtt tgccatctag agtgggtact ccaaatcatt
    25261 tattatcaca cgatacatcg agttatttat tatcgccaca atattctact gctataggct
    25321 tgcttttaaa tgctattaaa aatgattata atcagattat actaaataat agagtgaatg
    25381 aaaataaagt cgatggaatc aaagaaaaaa aacaaaaaaa atataaattt gatatttggg
    25441 gtaatttatc aaactttttt gattaataat aaaaaaatat aataatatgg ataaaaaatc
    25501 ttttgaattt attttgccag catcagatcc tcctataata aaagttatag gtataggtgg
    25561 agggggatgc aatgcagtca aacatatgtt tgacaaaggt ataaaaggag ttgacttcgt
    25621 aatatgtaat acagatttac aagtcttaga aacaaatcca attccaataa agatcaaact
    25681 tggtgcttca ggtctaggag cgggttctaa acctgaggtt ggtaaaaaag cagccgaaga
    25741 gagtattgag gaaatacgca acttattaaa aaataataca aaaatgctat ttatcacagc
    25801 aggaatgggc ggtggaactg gcactggtgc tgcacctgta attgctaata tagcaaaaga
    25861 attagatatt ttaacagtgg ctatagttac aacgcctttc atgtttgaag gtaatataag
    25921 atatcaacaa gccatgacag ggattgaaga attacgtaat aatgtagacg ctttactagt
    25981 aatacataat gagaaattat ttgaattgta ccccgaccta aaaatcagta atgcattttc
    26041 taaagtagat gatatcttat tgacagctgc taaaggcata gcggaaataa taactgtacc
    26101 tggtattgta aacgtagatt ttgaagacgt aaaaactgta ataaaagata gtggtatagc
    26161 tctaatggga acaggatatg ctgaaggaga aaatagagca atcgaatctc tcgaatcagc
    26221 cattcactct cccctgctta gtgaaattgc cattacaggt gcacaaaatg ttttactcta
    26281 catttcatat ggatctgatg agttaactat taaggagttt aaagaaatga catcgtattt
    26341 gcaaaatgaa actggtggtg ctaatgtgat ctggggtaat tcacataatc cagaattaaa
    26401 tggagtacaa gtaactataa tagctacagg atttaatcct acgcaatcta caactataaa
    26461 atcacaaaaa tatgaaaata catattgtga cgattcgaaa gtagccgaac aaaaaatagg
    26521 ttatggtaga aattatagag acgatgaaga acttgaaaaa cttgaaaata ttcctgcata
    26581 tattagaaaa ggcaaaaaaa taaatataaa tgcaccaagt ccaggcaata ttgcaataaa
    26641 tataaataaa gataaaacta taagtgaaaa taattatttg tataaacacg tcgattaaaa
    26701 tattaaaaga ttactcaaaa tattatttat aaaacttaag gtacaagtga agagtgaatc
    26761 aacaaataag attctttcta aatgagagta ctttattatc ttatctgcta gatcattttt
    26821 ttagatttac ctatttatca taggcataat agtattaagt tgtaaatatc cattcatgcc
    26881 tcttatattg atggatacgt cattgccaat tacttgagca caaaccatag taagagcttc
    26941 tgcttgtgta gaattcactt tgttggacat catgcttgat ccatgttcat tagtaggtaa
    27001 atttatttca ccaatagagt agctgggtcc actagctcac tgctgttgat tttagtgctt
    27061 cgctagcaga tactatggca tcacaagtag aaatgccttc aaatttattt tttgctggtt
    27121 tgaatggtag ttctgtaaat tctgcaattt ttttataaac tatcacatca aatgttttag
    27181 gagcatttag ttcagtacca acgactgtac ctccgatagg aagctctgat aaatgatcta
    27241 gggaatgttc gagtttttgt atattatgtt ctaattgtga aacataaccc gaaaattctt
    27301 gtcttaatgc taaaggaact gcatccatag tatgcgtacg accaatttta ataatatttt
    27361 tatatctata tttataattt atatttaacg tccttttcaa tgtttttatc gctctctact
    27421 tgtccttgct aaaatacttt tttgtctatc agtaataaac tatcggaaag attcattttt
    27481 atggatgttt tgttaactta tttgcaacca aaaagaacat ccatgtcttt caacaccaga
    27541 taaattagga tgattccata taggtgaaat aatctttgct aattctacat cttcgcttat
    27601 aaagtaattt ggcaaaattg ttttatattt ttctggattt gctaaaataa aagcagctag
    27661 catatattgt tcgttgtaaa acctggcaca catgaattgt ggataatcat aaggaagata
    27721 aatgtcatga atatgaacaa taacaccatt ctttaaaaat ggaagaagct ccaaaaaaca
    27781 aatcatggca tctgaattag gaaatactct atgcgaatta tcaatgaata atatatcatt
    27841 tttttctaat gatttaacaa taaatttgtt atcaggtaaa ttttcaaatg gttttctaat
    27901 aactttatct gctaaatgat caatattggc tcttggtaac gggtcaatcg aaataatctc
    27961 agtatttaga ttattttctt taatagattt atatgcaacc tttgtagaat ttccggaacc
    28021 aatttcgata tactttgctg gtttatattt tgcaatgata ccgtatatcc caacaatgtc
    28081 aagacctggg agaaatccat tattccaagc aggattattc tcatctgttt cttgtcttga
    28141 atctttaata tcatgaattc tctcattata tttcaagaat gattctaaat ggtctttgta
    28201 caaaagtctg ttcttattta ttatatctaa taaaagggta tgggggggtt accatgccca
    28261 taccttggtt taggaattac tttatattct agatgaatgc tctgaaattt tggtgatata
    28321 aatttatata ccgatttgat tttttccttc attactgatt tattttgtta atattatttt
    28381 acgaatttaa attaattttt aataaataat tcaatatccc cccatatatt tacgaataaa
    28441 ccattcaaaa ccgagtaata aagtaataat aactagataa atccaagagt ctattactga
    28501 gctagttatt ttttctatgg tataatattt ttggctcatt gtttgggatt ttaatatttt
    28561 ttctaaagaa tccaattctg aaaaatataa aaatttacct ccagaagcat ttgcaatttc
    28621 ttttaataaa tcatgattag cagtcaaatc ttgcaattct acgtctattt tttctactat
    28681 aaaaaaacct tttgctgtaa aggggaaatc aggtgataat actgtagcac tatagttata
    28741 aactccaggt ggcaactgtc ctaattgcag agaataacca ttacctgatc gcgaaaaaag
    28801 atatttatta gaatatcctt ggttagaatt tataattatc tgtatttctg gtgtatttat
    28861 aagttcgcca cttggattta ttaattcagc atatatattt attgggtcta cactttgata
    28921 tacagattct actattattt taaacccttc tttagtagta gttaatagat aatttattac
    28981 ttttatcacc caagtatcga aattattatt attatttctt aatgattcta ttctccatct
    29041 ccatataccc tcaccattga aaactgcaat atttttattt attgattgat aaaaaaacat
    29101 tacaggattt gtagtagtag cagtgccaat tttttgatag aatatggttg ggaattttat
    29161 gctagttcta ttacgcggat acaacgtgat gataggtggt gcttgttcaa aaaagtgcaa
    29221 ttcttcattt gcaatttgaa ataatggaaa ctgttcattt actttaactt gggattcgat
    29281 ataactacta tttattaaat ttcctatcca cgggactata gaagtagcta aaggcaattg
    29341 tgtctgagta ccaataaata accaaatggg tattgtagat tgttttaatt cctctatttt
    29401 gcttgtattt atgcaagcac tatttaattg atatataatc aaaacatcca aatcattagc
    29461 tataggatct ataccaggtc tgattaactg tacctcattt aataaaacac tactgagagc
    29521 tctattaata gcaccaatat ctgggtgagt agcacaagct aatataccta ttttgtattt
    29581 ttgttttaca acctctatac ttttgcttag gttattattt tttatatttt tctcttcact
    29641 aatttgttgc caagtaactt tgattttatg tattccctct ttttctgctt ttgcctggat
    29701 agttaatatt tttgagtaat tatcatgagt aatatttata ttttcactat accaaagttc
    29761 atcatctata aaaatttgta attttgatgt ttttccttta gcttttttag cattcaaaat
    29821 tatttctata ggaaaattgc taccaatagg tattttttca ttacattgaa ttttacttac
    29881 ttgcaagtct gtctgagtgg tagtgtctcc caaaccaatt gtataaatag gattcttgaa
    29941 tttgttaaat aagaatcttg gatcttgttt ttgtgtattt attccatcag acacaaggat
    30001 gatagctcta atattgtcat tttctaatga tttattaata gctagttgta atgcatcata
    30061 taggttagta gccttgccat caaaagtaaa taataactta tttgttataa cgttagagaa
    30121 ggaataatta tgccatatat aatttttgga atatttgtca gttaacgatt ttattttagt
    30181 acttatttca ttaaatgaat tgtctacagt aatagattga gaattgtcta ccaaaagaat
    30241 aatttcaggc ggatatacaa tttttttatt tttttgtata tagggagtta ataataaaaa
    30301 gaaaagtaaa aaaaatacaa tactacgtgt aactattaaa gtatttttcc atggttttga
    30361 taaagcatca taaggatttt taaaatataa taacaatgct aaagcaatag ataaaatagc
    30421 aaaaagcaat agccaccatg gactataagg aagtataatt ttcataattt acatacgcat
    30481 accgccacat acatttatta cttggcctga aacatagctt gatagctcac tagctaaaaa
    30541 taaagcaata ttagctacat catcaggtgt gccgcctcta cgaagaggta ttttttgcat
    30601 ccaatctttt ctaacttcat cggggagttg agctgtcata ggtgtctcta tgaatcctgg
    30661 tgctatagca ttacaacgta tattacgcga acctacttct tgagctatac ttttagtaaa
    30721 tccaataata cccgctttag aagcactgta gttagcctga ccagcattac ccgctacacc
    30781 tactacactt gccatattta taatagatcc agagcgattt ctcataaaat gttgtaatag
    30841 tactttggtc atattaaaga ctgattttaa atttactttt attaccaaat cccaatcttg
    30901 ctcactcata cgaagcaaaa gattatctct agtaatacca gcattattta ctaatatatc
    30961 tatagtggtg aaatcttgta aaagtttctc tacaaaatct tggcaagcat tcaaatcact
    31021 cacatcaaaa gcataggctt tagcttttat attgtatttt ttctgaattt cgtcaataaa
    31081 tttttctacc tcttcattga tatttaaatc tgtaatggct acatttgctc cattacttgc
    31141 aaatttttct gcaatagctc ttcctatacc gcgagccgct ccagtaatta ctgctgtttt
    31201 gttttctaat aatttcataa tctttaactt tttaattgca aagataaaag taaaaaatca
    31261 attatttaag ctttgatttt atttaaaaca aaattataaa tttcgttaac ttctttttca
    31321 gtattttcta ttatttgagc tgaatcatct aaaataggtt ttgcccaagt tttgtcatcg
    31381 agtcctgcaa catttataag cacattcata tatgcacctt tgatagctgt taagcaagct
    31441 atagcgccta caccagcatc agatacggaa ttaggattac ctatttcagc cattttttta
    31501 cacaatggta tagctaaaga agctgttttc aatgtgcggt atggaatctc tatggcatat
    31561 cgagtagcct cttcgatagc tttttttctg ttaatcatct gctcttcagt atcttttggt
    31621 aatccgaaag catccattac tttattgaaa gcattagcat cttcttctag gagctgtatt
    31681 aattcctctt taatcttttg cccttcttca gcataattac taaactcttc ccatctatcg
    31741 tcccagccac gtttatgaga acttagatta gcaaccatag tagctagagc agtacctaaa
    31801 gctcctacat aagcagatac agaaccaccg ccaggagttg gagattcact agcggtctca
    31861 tcagcaaatt cacacaaaga catatttatc aattttggtg gaggttcatc ttttgctaaa
    31921 ataaattcta ttactttgga ttgaggaaca aaaggtttca aatcatctag ccccatagat
    31981 ttaatagcaa tctttatcaa ttcattttca gaaacaccag tagaacgttg ttgctttctg
    32041 agataatatt tgcctgcgtc tattaaaact gatttaggta taaggccaac aatttcagca
    32101 ccagtaactc taatacctcg taaacgagct ttttcactaa cctcatcaaa agctatatga
    32161 accggggtca ctgtaatatc tgtgatattc atagacactt gggctatacc atattcttcg
    32221 atgaaccatc ctatagcttt agtagctttc aaagttccag gaatatatac tgtatttcca
    32281 ttttcatctt ttacgatttc accagtaatg gagttgcctt tacgcatagg tcttcctttt
    32341 tctcgtacat caaaggctat agcattagct ctacgtacag aggtagtgtt caaattaaaa
    32401 ttaatcgcta ctaaaaaatt acgggccccc acagctatag ctccaatttt aggcaaaaaa
    32461 ttagctggac catagtcggg atgccagtga gggtctttta attttttttc caaaccttca
    32521 tattcaccag ctctcacatt tgctaaatta cgtctttctt cagtttttgc agcaaattca
    32581 taaagataca caggtatccc tagttcttcg cctattctct tacctaattt atttgcatat
    32641 tctactactt catccattgt aatattagct actggaacta atggacatac atcagtagct
    32701 ccgaagcgtg gatgggcacc tttgtgtcta gtcatatcta tcaattcggt agctttttta
    32761 atagctctaa atgcagcttc tataactgca tcaggttcac cagcaaaagt tactaccgtt
    32821 ctattagtgg tagctcctgg atcaacatct aaaagtttca ccccttcgac attttttatt
    32881 tcttcagtta tttgattgat gatgtttaaa tctctaccct cactaaaatt tggtacacat
    32941 tcaataattt ttttatttaa cagcataatt tcagtttttt gatttttatt aattcgttta
    33001 cgaaattaat aaattttaat ggctaaataa aatttttgtg aattttctaa atttttgatt
    33061 tagaaattct ttgatacaaa aaacattggt tctataacgt ttattgtggg taaaaacatt
    33121 ttagattatt acaaaaaaat aactattttt taattattta ggttattttt ttaccacaaa
    33181 gggcaccaag ttattacaca aaactcacaa agtgccattt attgtccttt taatttcatc
    33241 cttctctgtg ttctttgtgc attcattgag aactctgtgg ttaatattta actgtacaat
    33301 aataaataaa ccaatattga caaatttatt tttgtaaaat tagttttttt aatctttacc
    33361 tatataaaac gttatagaac caaaaacatt ggttgaattt gtaatttatt attatataaa
    33421 taatttaatg ataggagaat ttttttttgt tattatctaa aacatctatt tcgatttttt
    33481 tatttagtta aaattaatga aatttgcaaa aaatctatgg ctatagaact aagtgaacaa
    33541 gaaattatta gacgcgaagc tttaaagcaa ttacgtgaaa tgggtataga gccatatcct
    33601 aatgaagcat atactgtcac acattatgca aaagatatat tagaaaacta taaagaagat
    33661 tccactcaat atggtgacgt atcattagca ggtcgcataa tgagtagacg tattatgggg
    33721 ggagcttcat ttatagaaat acaagatagt actggtagaa ttcaagcata tatcaagcaa
    33781 gatgaaattt gtccaccaga tgataatact ttttaccaag ttgtattcaa aaaattaatg
    33841 gatattggcg atatagtagg tatacgaggc tttgttttta gaactaaaat gggagagata
    33901 actattcatg tgaaagaact aaaattacta gctaaatgct tgcgtcccct accaatagta
    33961 aaagaaaaag atggaaaaat ttatcatgct tttacagatc ctgaacagag atatagaaat
    34021 agaactctag atctgatagt caatccacat gtgagagata tctttcgtac tcgtactaaa
    34081 atgatacaaa gcatgagaga ttttttgaat aaaaaaaatt ttatggaagt agagacaccc
    34141 attttgcaac ctatatatgg tggagctaca gctagaccgt ttaaaactta tcataatact
    34201 ctcgacacgt cattgttttt gcgtatagct aatgaactgt atctgaaaag actgatagca
    34261 gggggcttcg atgcagttta tgagtttgct aaagattttc gtaatgaagg aatggaccgc
    34321 tttcataacc ccgaattcac tcaaatggag ctttatgttg cgtataaaga ttattattgg
    34381 atgatggatc tagtagaaga aatgcttgaa tacataactc tcgctataca cggcaaaact
    34441 gatattttag tcggagaaca cacgatctca ttagctagtc cttttaaacg tatttgtttt
    34501 tttgatgcca tatacaaata cacaggtcac gatatttcaa atttagatga aaatgaatta
    34561 attgaagttg ctaaaatatt ggatattgaa ctcgatggtg taagtggtaa agctaaaata
    34621 atagatacca tatttggaga taaagtagaa ccacttctaa tacaaccaac ttttataatg
    34681 gattatccaa tagagatgag tcctttagca aaaaaacatc gttctaaacc cggattagta
    34741 gaacgttttg aattaataat aaatggtaaa gaattgtgta atgcatatac agagcttaat
    34801 gatccactgg atcaacgtca aagattagaa gagcaattac aactcagaca actaggagat
    34861 gaagaggcta tgatgctaga tgaagatttt ttgagcgcta tagaatttgg gttaccacct
    34921 actgctggac ttggtatcgg tattgataga ttagctatga taatgacaaa tgtaccatct
    34981 atacaagagg taatattttt tccacagatg gctccagagc gtaaagctat agctatggat
    35041 tctgaaagcc taaaatctat agggatacca gatgtatgga taccactact tacggaatct
    35101 ggtattgtaa ctttagaaga tctaatagaa actaatgtta ataaaatttt gaacagttta
    35161 ggtggtctta gaaaaaaaca taaaatcaaa gattctcttc ctacaaaaga agacatagaa
    35221 aaatggataa aaaatgctga aaaatcaatc aaagaatcaa aataaatgat tcgatcatta
    35281 aatataatct aaaccacaaa tttatagtga attaatcaga taaactatga taaagtaggc
    35341 aagataagaa aagcagaaaa gttgtaaatt atttatattt aaataaaaga tttaaactca
    35401 ctcatttcaa taaatcaaga ttacaaaaat caagataggc acttagatat taagtgtaaa
    35461 aaaaaacttc aaaaaaaatt tattttgtta aaatcaaaaa taagatttgt aatattttta
    35521 attttaaagc ctattttgat ctttttacta aaattaatta atttagcaaa aaaataatta
    35581 atttagcaaa aaagtaatta tgagaaaaaa aattgttgca ggtaattgga aaatgaatcc
    35641 caattatgac gaagctatcg atttattaaa ttttttaaaa gataatatac caacagatca
    35701 ttctggtgta gacattatta tttgtccacc atttatctat ttgatagaag ctaaaaaaat
    35761 attaaatcgt caagatatat acgtaggcgc acaaaatgtt tcttctttta ccaatggagc
    35821 ttatactggc gagatctcag cagctatgct aaaaagtata aacatcaatt atactattat
    35881 tggacattca gagagaaggc aatattttgg tgaaaaagaa gaaaatttct caaaaaaaat
    35941 aaaactttgc ctccaagaac acatcacacc catttattgt gtaggagaaa ctctagaaca
    36001 aagaaataat ggagagcaat taaaagtagt agaaaaacaa atatccgagg ctttgtataa
    36061 tctatctgat gatgaaatag ccaaaatcat cattgcttat gaacccgtat gggcaattgg
    36121 cacaggagta aatgctacac ctgatcaagc acaacaaatg catgtattta ttcgtgaatt
    36181 gattgcaaaa aaatataacc agactatcag tcaaggtatt ataatactct atggtggtag
    36241 tgtaaaagct agtaatgcta aagaactttt ttctatgcct gatatcgatg gcggtctcat
    36301 aggaggagct tcgttaaagg gtaaagaatt tacttctata attaacatag ctatgaatat
    36361 ctaatctatg atatggattt ttatcaaaga ggctatagcg atggcctggc attccctgaa
    36421 gtctaataaa ttacgaacaa ttcttactat ttctggtatt actataggta ttttttccat
    36481 tatagcagtt ttaacggtga tagattctat taaaaatcaa gtaaatactt cgatagcatc
    36541 tctaggtagc gatgcactct tcatacaaaa gtggccatgg tcgtttgatg cagatatgcc
    36601 atggtggaaa tatgtatcta gaccaaatac atcctatcgt gaataccttt ttttaaaaaa
    36661 ttattctgaa acaataggcg acattacata tacagcacta ttgaatacta aaacttcttc
    36721 acataatata actaaatcta atcaaagtat aataggtatc actacatctt atgcaaattt
    36781 ttttcctttt attataaaag aaggaagaat gtttaccctt gaagaagtag agcaaggtag
    36841 tagtgtttgc attttgggct atgaagctgc tattaattat ttcggaacta ctaaaataat
    36901 cggccaaaca attaaaattg gttctcaaaa attaaatatc gtcggtgtat tagaaaaaga
    36961 aggagagaat atcctggctg gttcgaacga taaattgatt tttgtaccag tattatttct
    37021 tagaaatttt ataaaacttg atagcgatga agctaaccca tatatttctg taaaaccaaa
    37081 acctaattat actcaagaag atttaaaaaa tgaagtaagg tttcttttaa gacaatatcg
    37141 tagattgcct cctaaaaaag aagatgattt tacgataaat actgctacaa tgcttactca
    37201 aagtgttgag gggattttta aaagtatgac tttaggagga tggataatag gtggatttgc
    37261 tctattagtt ggtggatttg gtatagctaa tattatgttt gtctctataa aagagagaac
    37321 tcaacaaata ggaattcaaa aagcattggg agctaaaaat agatttattt tgttacaatt
    37381 tttatttgaa tcagtttttc ttagtattat tggaggttta ataggtctat tactagtttt
    37441 aatcttaagt attgtattaa aatttttaat aaattttgct tttccactta cttttgaaaa
    37501 tatcattaca ggcatcataa tagctaccgt tactggcatc ctcgctggta tagttcctgc
    37561 acttcaagct tctaaattag atccagtaga agctatgaga aaaaatgtat gagttttttt
    37621 tcaaatatgt atgtaaaatc aatctacaaa aaaaattaga ttttcaaata ttatttttat
    37681 acgtttgtaa attatcttca gttattttag aatttgttca cttataaatt ctaaatttat
    37741 tattattcta atcttttaat ctttaaaaaa aatctattgt tcagacattt ttgatttatt
    37801 ttttaagtaa tttgttttat tattcttttg ttacttttag agatttagta ttaataatat
    37861 ttctatcagc aaaaatttta tttgaaaaaa aaaatattta tatatttgca aaacaatttt
    37921 taaaaattac aaggttatga aaaaattaag tgttatttta ttaatagtta tcgcatcaat
    37981 cactattagt ggttgtaaag acaaagacaa aaataatgat tctcaagaag caactaagac
    38041 aacactatta tgtaaaaaat ggaaaattga aaaaatttat atgaatggtc aagagaggca
    38101 agacttgtta aatttgatgt ctggtatggt ttgggaattt aaaaaagatg ggactttcac
    38161 catttattta ggtactagca ttgcatcgtc tggtacttgg aattttaata atgatgaaac
    38221 tcaaattaat ttaacagtaa cctatgagca aggcgaaaat ttagaagaac ctattaatga
    38281 tattttttat attttgaagt taaatgaatc tcaactatgg gttagggacg atcaaaatag
    38341 cgattccttt gaatatcatt ttattccttt gacataaaaa atttattaaa atagatggtt
    38401 ctataacgtt tattgtgggt aaaaacattt tagattgtta cataaaaata actatcttta
    38461 atttaggtta atttttttac cacaaagggc accaagttat tacactttgt ttaccctttt
    38521 aatttcattc tcctttgcgt tcttggtgcc ttctttgaga actttgtggt taatatttga
    38581 ctatcctata attaataaac taatattaac aaatttattt ttgtaaaatt attctttttt
    38641 tattctttaa cccatataaa acattataga acctcaattt ttttttaatt agttcaaaat
    38701 tccattccta aattaaacat cataaaagca aaaacgtcgg caacctcatc tattgttttg
    38761 gcaataggtt tgccgccacc atgaccagca tcagtttcta tgcgtatcaa tactggatta
    38821 tttcctgttt gtttctcctg tagagtagcg atgaatttaa atgaatgagc gggtacaact
    38881 ctatcatcat gatctgcagt ggtaaccaat gtagctggat attctacacc ttctttaatg
    38941 ttatgcaaag gtgagtaagc gtataagtat tcaaaatcat taggatcatc actactacca
    39001 taatcagaaa tccatgccca tccgatagta aatttatgat aacgaagcat atccattact
    39061 ccaactgctg gtagagctac tttaaataaa tctggtctct gtgtcataac agcacctact
    39121 aataaaccac cattagatcc accatttatt gccaatttag agggcgatgt ataacctcta
    39181 gaaatcaaat attcagcagc tgcgatgaaa tcgtcaaata cattttgctt tttttcttta
    39241 ataccagctt cgtgccatat tttaccatat tctccaccac cacgtatatt tggtacagca
    39301 tatatacctc ctcgttctag aaatggaatt attttaatac taaaagatgg caagacacta
    39361 atattaaatc caccataacc gtacataagt gtcggattat taccatctaa tttgatatct
    39421 tttttatgcg ttataaacat tggaatttta gtgccatctt ttgatgtgta aaaaatttgt
    39481 tcagttacat aatcatcagg attaaatttt aatgaaggtt tgaaatatag acttaactga
    39541 cgtgtatctg cattataata aaatatagtg ggtgggtata aatatgatgt aaaagtgaaa
    39601 taaatattat tatgctcatg gtacgtactt atacccgcca cagtcccgat agttggtaat
    39661 ttaatttcat ctaatatttt tcctgtttta tctgccagat ataatttact tttcacatcc
    39721 tcttgatatt gtataaccca ataatctttg ctagcgaata ccccatttaa tacaaattta
    39781 gattctgcta tcactgtttt cggtgaaagt gatggttttt ttagtgagat ttttattaat
    39841 ttgtaattgg gggcaccttc attagtaatg aaataagcaa aatcatttat tatatctact
    39901 aaatagtaat cgtgttcgaa gcctttgaat aataagctaa aactattatt ttttagtggt
    39961 agtttagata catgtagttg tataccgcta gtagattcat tctcatataa aaatgcatat
    40021 ttctcatctt tgtcagtata taataaaaaa ttgcgtagtg gataatcatt attttcataa
    40081 atcaatttat ctttattctg ttgagtacca attttatgat aataaatttt gtgatattca
    40141 tttttagctg ttagtgcttt ggtagagtct gtgatctcat aaccactata gaaaaatcca
    40201 tctttgtacc atgctatact agtaaattta gcccatttga tatgatctgg taataatttt
    40261 ttggttttca aatccattac gaatatttct acccaatcgc taccagcctt agaaatgcca
    40321 taggccaaat attgcttatc atgtgaaaaa gctaatgcac tgatagctgt agtgccttta
    40381 ggatcccatt tattagggtc gagtaatact agttcttcgt tagttttttt atttttttca
    40441 caaaaaacac tttgattttg taatccagaa tttttgaaat agtagagctt gtctcctatc
    40501 tctgatggag tccccatacg ctcataattc catatctctt cataatattt tctcaattta
    40561 tcacgaaagg gaatctgtga aagatacgaa aaagtgagtt cattttgttc ttttatccac
    40621 agcttagttt cgggactatt ttcgtcctct aaccatctgt aaggatcaga tactaaggtg
    40681 ccatggtagt tttctactac atctactttc tttgtttttg ggtaatttag ctcttgagag
    40741 atagtactat tcataattaa aaacattgta ataaaaaata atagtttacg cataataatt
    40801 tttttacaaa tataatttaa aaaattttaa aataagctag tttttgtgca gaatattttt
    40861 tttacaatta ttttttagta aaaattgtga tttttttaaa aaaacagata tatgctgtaa
    40921 tcgtgattat cacaaaaaaa taaaggaatc atataaattc aataattact tcttttaata
    40981 atttttcttc taactgacgt gctatacgtc taaccactgt ccttctaatc tctaaatcta
    41041 ctggtaaatg tggatcttgg tgagcttcat ttaccttagt accaactaaa aaataaattt
    41101 catcactgtc tattagcagt tgtaataatt taccagcggg accgcgaggg atattaaaag
    41161 gtggattttg atgaaataaa atatcagaaa ctttgctaag agtaagaata ccttctgtta
    41221 taaaatttgc accttccata taagatatag gaggtaattc tgggtcttca aattcaaaag
    41281 tgtctataat ttttttgttc aattctctac taattatatc cgcagtagta gcgccacaga
    41341 tgactttatc tcctttgaaa tcttgaaaaa tctgagccat ttttttatca tctttctcat
    41401 caaatggagg cccagtaact atcaacactt tacgcggctg tctgaaatat attactccac
    41461 aagtcatatc atcttgagga ttataattgt caagtttata tgctatgttt actattcttt
    41521 gtgccaattt ttgagcagag attaatggtt cggattctaa tgttttactg acaaaatcaa
    41581 caacattatt tctttcccaa ccaaaaggat atttgtcagt acccatacca gcttgtgtaa
    41641 tgccatctgt taaaagaata agtctatctt gtagttgagg gtaaaaagta gcatgcttta
    41701 tttctctttt actattctca ttttttaatt cattgattaa taaacaagta tgaggtatag
    41761 taagttcact attttttcta aatattatgg tatcaggatt atcatgttct aaaattttaa
    41821 cagtactatc aggcaaaata tcacaaatgg taaaagtagc atagctgatt tttctttcac
    41881 tacatacagg aagcgtattt aaaataattt cagcaatacg aaattcttct ttatgttcat
    41941 aagtgaaatt taaagccata gtagcagtca gagcagatag tacattagct ttgacaccgt
    42001 gccccatacc gtcagacaaa actactatat atctattctc ttctttaaat cttttataaa
    42061 gaaacgtatc tccacaaata tatgaatcat aatgatttct ataagaaaat cctacttcta
    42121 caaaaatatt atttttattt atctcacttt tcataaattt taataacttt atttaagata
    42181 cgttctactt cgcttgcacc ttcgcccaat gaaaaagcaa tttgctgtac catagccaac
    42241 tctttttcta tagctttttt gatttcttct actacttcag ctttattaac ctctggaata
    42301 ctaatatctt ttatcaacat cactgcaagt gaacgatatt taataggata tacgcttaca
    42361 ttaaaccact tattatttat taatatatct ctttgagttt caccttgagg atgtgtgaga
    42421 gcatactcta tcatatttac tagtgatata tctagtaatt tttctatttg agaacctcga
    42481 agtccaggca ctatctcatt tatttctttt acctcatcac ctaaaagttt tacaaaaaat
    42541 tcattagaat attctacttt gttttctcca tttactacta agatgccagc gggtacttta
    42601 tgcaataatt ccttgatatt ttctattatt aacgcatgta gttgcaactc ttcttttaat
    42661 ttttctcctt tttcctcttc agctacgatt ttttgattta atttttctaa ttgattattt
    42721 aattttttaa tcaatttaat tttattagca atgttataat aaatacacat ctccgaatta
    42781 gcaataccct gagctatggc ggtagcaaac tcttcgcaag tatcgaaacc acaattttta
    42841 caaggtttag cactgggagt tatttgcaaa tagtttaata tgtcttctat ttgtttttta
    42901 gacactttgg gcaattttat ttcaagattt ttgaatgata ctggtaaagt ttcatttata
    42961 tatttattaa tatttaattt ccatttttct tcatctaaat ctcttaatct cttttgagaa
    43021 tattgtaaaa cttgatattt tttcattata taattaccag tcaaagtaat cgcaggtccc
    43081 ataatacaac cattgcagta gtaaatatta aaatgttttt taattatttc gatataattc
    43141 ataaaatcga aaatagcatc tttaaaaaga ttttcgccac tactagaaat cacattagta
    43201 cttaagatat cttgcgcttg atctgttgct attaatatac catcttctat tgcaaaataa
    43261 ttaccccatt taccttctac aaaatcaaaa gacgaaaatt cttgttctat ttcagtgatt
    43321 ccaaatctat caaataattt tcttaattct tcaaatgtta aaacctcatc actattacta
    43381 aattcactta tatctttata agctatacat ggaccaatat acacagtctt aatctgctca
    43441 tttataattt ttttagtaat aaatgaagac atttcaggta aaataggaag aggtaataga
    43501 ttattaagag ctttaatagc atatttttga acatagtttt ggataatagg acaaagtgta
    43561 aaaagataat atttgccttt gttttcgtct aaaaattttt tatattgaat agctagtaaa
    43621 tcggctgcaa aagctccatc tattacataa tcaaatccac tctgccgaat catagaaata
    43681 aattttttat aatcattgac atcaataaat tcaccagaaa ttgctggatc taaaatagct
    43741 gctaattttt cttcatcatt tatccaagat ataacttttt cgatagaatt tctataagta
    43801 atagcctctt gtggacaatg tgtatagcag gatccgcaat taatgcatct atctgctatc
    43861 aattctgcat gagtttcttc tactgaacta cgtatagcat gtacagggca tacctttata
    43921 catgcgtagc acatattaca tttagaagta tcaatctgta tttgcgcttt catgaatact
    43981 aaaataattt aaaataattg attttatatt ttctggatta acgcgattat aaatctgatc
    44041 accaatttga atattcggac catctgcaca tctgtcgaaa catcttttac cacgaaaatc
    44101 tactttatct tgcagctgat attgctcaat agtattattg ataatttcaa ccaaaattct
    44161 agctcccttg ctaaaacaag aactacccat acaaatgtaa ataggtattt tgtcattatt
    44221 tttttcggtc atccaaaata ttttttcaaa tatacagaaa attactttat ataaaatttg
    44281 gtttaaatca tatcaataag gcaatcaaat tattatagta atgataaata aaaaaaaaga
    44341 atttattaaa atcatattaa attcagcaca aaaaaatttt aaaattgtaa aaatatatta
    44401 taatattttt taaaaaaaaa ttatttaata aaaattattc tatagaatga ggtagcattt
    44461 acatattatt caaagattcg agtattgttt gatatttatc tttattagct tctacagaat
    44521 aatacatttc aacagttttt ctagcatttt tgcctatttt ttctctcaac tctttatttt
    44581 ctaaaagttc tattattctt ttaatccaca tatcaatatc tcctacaggt attagaaatc
    44641 catttacacc atcattgatt attctaaaat tagctccaat cgctgtagca atcgtaggta
    44701 tacccaaagc catatattgc aatgctttta atccactttt accatatacc caaggctcat
    44761 ctggtaatgg atataaccca atatctattt ttattaaatc atttacttct gtatccaaac
    44821 tccaaggtat agctgtaaaa gggatctcag tattcgaaaa tcgaaaattt tcatctccta
    44881 ttattaatac tttaaatttt attgatcttt tcaataactc tacaaataca ggttctaaaa
    44941 tttttaagta tttagaagta ctatgactcc cactccaacc taaagtgggt aagttatgca
    45001 aggtataatc tgcaattggc acgtaaatgg cagtatttat agtagatgat atatctgtgg
    45061 tcatagagtt atattgcttt acaaatttat ctaaggtagg tgtacatgta atcacatgat
    45121 ctgctttttt cataagagtt atcatctttt tagtaccttt tgcttttata ataaatttat
    45181 tagcatcact cgaatgcccc aaaaatatca tatcatctat atcatatatc attttatttg
    45241 ataatttagc aaacaaccat tcaaatatag gtaaacctat tggcgttacc cataaatgaa
    45301 tatatataat tgaatatttt ttaatacgca ataaatctat caatcgtata aaatacccat
    45361 aaatcgtata tattattttt tcaaaaaaat gtcctttttt atatattact ttccaaaaat
    45421 tttcagttac aaaaggtgaa actgtgattt gccatccatt atcattaaaa taattaaaat
    45481 attgctcata ttttaaacgc tggcttggag ctttatcggt aggatatgga caaattatta
    45541 gtattttctt atttttattc attaataaaa attattaaac aaaagtaaaa gaattttttt
    45601 taataaatct aaatagcatc atacacatct atatatttgc tactcagtaa tgtcagatca
    45661 tatttttcac gtgccatagc tctaatgtac tgcgagtcaa aatctgtgtt ttttagtttt
    45721 agacaagcac tacggtattc ttttttatta tgatttttca caaaaactcc taattttttc
    45781 tcttctaatg taacgatggc ctctgaacta gtattagtaa ttataggttt acccatagct
    45841 aaagcttcag cattttttat aggagatata cctatattag agaaattttg atttaaaaaa
    45901 aatacaacat gcattgcttg gtttatatat ttaggcatag atgcccttgg tatattataa
    45961 ataatttcaa cctcgttata ccatttgtat ttatttaata gttctaatat tttattgttt
    46021 ttactatgaa gaattaaggt aaactttgat ggaataaaaa cccgtctata ttcattgtaa
    46081 aactcaaaca tttcatcaaa taaataccaa ttttcagcag aaccaaaata tagtaaatta
    46141 aaaatatgat tgtcatattt tatctctact ttgtcagggt cgaaaaaatc aaaatctgta
    46201 gcacatggta ttacataaat cttattagaa gcaatagaaa atttgtcaat aataaaatct
    46261 ttagccgcat cagtgagcac tactatagca tccgaatttt ttaaccataa tttttctttt
    46321 ctcttaaaat atttatatac tatcctataa ataggatttt tcaaagacca tatatgccca
    46381 tctactcgct catctgccca gaagccacgc atatcaaaaa taatgggaat attatacctt
    46441 ttagctgcag gataagccat catcatagat atatagctac gagcatggat acacaaaatt
    46501 tctggattat attttaatat ttttttaatt ttttccctac ccctatcgat atcatataga
    46561 gcagataata tagctggttt tttagtataa tcatcaggat accacaccca attatatttt
    46621 tctactttac tccacaattc atcttcaagt ttaggcaact tttctttttt ttcgaaactg
    46681 agaatatgaa attttatagc attattagat aatttttcta aataaggaag tatttgagac
    46741 tgagataccg gatcagacaa tccatcataa gtaacgtata aaacattttt aattttatta
    46801 ttcataggtt atgtttttct aaataataat ttaaaacaaa taaattccat attctaccag
    46861 atagatacag aatatttttt tcataaaaat ttatcaataa tttatttaca aattgatagt
    46921 ttacaaaatt aaatatatta cttttaggat tgcataaaga acttttaatg tatggagcca
    46981 aatcattcat acaccactca gttataggga tagcaaaacc ttttttaggt ctatcaaaaa
    47041 gctcacgagg caagtaatca gccaagatat cttttaacat aaacttcagc tcatgattac
    47101 aaattttata attagtaggg atggaccatg ctagatctgc taattcggca ctaagcaatg
    47161 gaggacgtat ctcaagtgca tttgccatgc cagctctgtc tatttttact aataaatcat
    47221 cgggcaaaca ataattaaaa tcaaaccatg cctgctgcaa aatgggatct ttattatcta
    47281 gagtagaaaa aggcaattgt aaatcattag aaattatcaa tagttcttta agttgccaat
    47341 tagtaaaata attattttct attgaaaaaa tatttgaatt cacataatta tctatttcaa
    47401 aaaattgtga ccatcttcta aatctctgta ttggaatatt tttgaaagca aatgcaatca
    47461 aaggctttgt agacttaata aatggattat ttaacctttt agcccaccta tacataccat
    47521 aacccagaaa tagttcatcg cctccatcac ctgatagaac agcttcgact tttttactag
    47581 caaattgaga aatagcataa gtaaccaaaa aacttgaatc tgctaatggt tcatccatat
    47641 tatcaagtat tttttcaaaa ttttttgtta aaaaatcaat atctattttt aatggagtat
    47701 attgtagtcc aagataatct gctattctac tggcatatat actttcatct aagcttttgt
    47761 catcttgtgt tatagtaaaa gccatcaaag gacgattttg atatttagcg gctatactgg
    47821 ctatcaaact actatctatg ccaccagata aaaaaatacc catattagca tcactagaca
    47881 aggcacctct cacttgtttt tctatggtta tcctcacttg ttctttagct tctatataag
    47941 aaatattaga tttttttatc tcaaaattat gatatttttt aagtttatat ttaccatctt
    48001 ttattatcaa ataatgagcc gcaggtaatt ttttaacttg tgataaaata gtatgtgtgt
    48061 gaggtataaa gcctaaatgc aaataatatg ttatagctgt agcatctaca gatatttctg
    48121 gtaaaatttg ataaaaagct tttagctcag aggcaaaaca aaaataatta tcctgatgag
    48181 aaatgtataa tggcttaatg ccaataggat ctctgagaat cacaagtttt ttttctttct
    48241 catcccagat agccatacta aacatgccat ttagtttagc aaaaatagat tcaccttcta
    48301 atgcatagag ttctaggagc aattctgcag tagatgtagt ttttaaaaaa attgattggt
    48361 tgggatattt tttaaagaaa taatttttta tttcatcaaa attaaaaagt atgccatcaa
    48421 agacaattgc atgatgacca tctagagata ttaatggctg atcatttata tctaagctac
    48481 taccttgata ccctaatgca atatttgatt taaagtaata tgcagcggtg tcacgtcctc
    48541 tatgagctat atattcatta aaatgataaa tattttgtaa aaaagaatca ttagcttcta
    48601 tagaatagat accggcaatt ccagacataa tttaaccttt tactactgct aatggataaa
    48661 gtttataaat cggtttggta aggtcttttt gctgttccat tacttcatca atatccttgt
    48721 aacattgtgg agcttcatct agatctcttg gtttgtagac agaatgtatg ataccctttt
    48781 catcaagatt ttttttaacc tcatctaaat ttaatttttt aacagcggct cgtctactca
    48841 taactctacc agcaccgtgc gagctagaca tgaaactcat aggattgccc tggcctctta
    48901 cgatgtaact atgactaccc tgtgaccctg gcactatacc taattcacca tctctagcag
    48961 atatagcgcc ttttctatga atccataatt gttcaccaaa atgttgttct aaattagcat
    49021 aattgtggtg tatagatatt atatcaccga aatcacattt tagataatca taaaaaatca
    49081 tttttatggt atccaacata taaagacgat tttcttttgc aaattgaaga caataattca
    49141 tctctgcgat atattgttgc ccccattcag agtctatatg tagaaaggcc aaattccatg
    49201 aatctggtac tatattattc cactttttat tccattcttt tgctttattg ttataataat
    49261 ctgctacttt tttaccaata ttacgactac ctgagtgcaa cataatccat aaaaaattat
    49321 ctgcatcacg ttgtatctca ataaaatgat tacccccacc caaggtaccc aattgataag
    49381 gaatttcatt tttttctgat tgagttatta taccaggaaa ggtttttaga tgttgcggca
    49441 tgggttgagg ttttttatga tgtttgaaac caagcggtat agcttctttt atttgtatta
    49501 ctattttttc tagtattttt ctatttatat tatcatattt aatattagtt tttatagcgc
    49561 acatgccaca tccgatatct actcctactg cattgggaat tatcacctct tttgtagcta
    49621 ataccgcacc tatgggcata ccgaagcctt catgtacatc tggtaacaaa caaatatgat
    49681 gctcagcaaa aggatgttct gctaaatgca aagcttgctg catagcgcca tcttctactt
    49741 cagtagccca aatataaatt ggtaattttt ttgtctttat gatctgcata ataatcttaa
    49801 taatgcacta gtatttacaa agttaataaa ttttagaaaa attaaagtaa aattagttat
    49861 attaaaattc aacaacataa tttaccagtg atgttatata aattatttta tgagtaattc
    49921 gtagtgataa caaaaattta tttttcactt tctagataaa aaacttctct atttaactca
    49981 cttctcaata aatctaaaga ttttttaata gcaggtacat ctttttttag ataaagttca
    50041 aatattttca ccgcaaattc tgaataacta ctatgagctt cattaaaagc ttgattaatt
    50101 atttgaatta tctcttcttt tgcagaaata acagataacc aagctttgcg actaacataa
    50161 atttgttgac tcagattgta ctcatattct tgccttattg tatctaagat atattgatgt
    50221 agagttgtta aattttctgc atcttctttc gacattttcg ctaataattg ctctggtgct
    50281 attctttcta aaaataatgc tagcctctca taagcttgaa tttttaaagg tagaaaagta
    50341 ttttttatat catcatttct caataaacgt atctccttta aatgcctttc attcatagaa
    50401 tgcaaaacat acaatgctgt taaaaaaact attaatgatg gtattagaat taataatatg
    50461 attaaaattc cttgattcat ttttttgtac aaaaatacat aaaatttcaa tttataattt
    50521 ttatgaattt tttgtaatta atttgataat atttttttaa ttttgatagc tttataaatt
    50581 attttttata agcttttgga ataaagtaaa tcattatgtc tggaatttta tatctaatac
    50641 caagccccat tggcagtata aaagaggtag attatttaac acaatcgaat acagaaatat
    50701 tacaaaattt aaaatatttc atcgtagaaa gtgaaaaaac agcaagagct tttttgcatt
    50761 attgtgggca taatttagat gataatctta atttcataga atacaatgaa cataatttta
    50821 ataaaattaa cattgatgat tggttaaaaa ttttaaaatc taataattcc attggtttaa
    50881 tatctgaagc tggtacacca tgcattgcag acccaggaag tgaaatagta cttagagctc
    50941 atttaaatga tattgaagtc tatccttttg caataccatc atctattaca atggcgctaa
    51001 tgtctagcgg attgaatgga caaaaatttt catttcatgg ttatttagat aaaaatgtca
    51061 ataaactacg acaacaaatc aaaattttag aaagagaaag tagactgtca ggttattcac
    51121 aaatatttat ggaagttcct tataaatcgc aaaagatctt tgatttatta ttaaatacac
    51181 taaaatcgga tactttttta tgcgtagctg ccgagctgaa tacttctgaa caatttataa
    51241 aaactaaaca aataaaatca tggaaaaatt ttcaagtaga actaaataaa aaaagatgta
    51301 tttttatttt tcaaaataaa aattattttt aaaaagatag tgttggtaat caaattttat
    51361 tttatcacat aataagtaaa ttgtattaat aaaatttata acttattgct tatcttttag
    51421 cgagttgaat tttcttagtt attactttga ttataccaat agcaaaattt gctcctttat
    51481 agaactttaa aatattttaa aaattaatta atcaattttt ttgtttaatc atatctcggc
    51541 tgatatccaa taatttatct ggtaataaat ttttcataca agcaaaatgt tttttaggac
    51601 atttatcatg gccaagttta gagcatggtc gacaagatac agaatcatct tgaaaatatt
    51661 tatgcaaaat tttaacatta tcaggatata atggatacat accaaactct ggtacagtat
    51721 ttccccaaat agaaatcata gatttatgta gtgctactgc cagatgcatc atacctgtat
    51781 cattactaat aactaatatt gaatttttta ttataaatgc agattctatt aatgacaatt
    51841 taccacagta attaaaaact ttattaccga cctcatcttt tatttgattt ccttttacct
    51901 cttcattttt tccacctaat aaaactacag ggtaatctga ttttttacaa tattctatta
    51961 catattctgt cggatattgt ttggtataat atgtaccact tatagcaaat gcaatatata
    52021 ttggcggtaa ttcataatta aaaataaaat tttctggaaa aaagaaatct aagccattgc
    52081 catcgtagta aacatctaaa tatttagcag attcaaaata tcttaatgct acattagaaa
    52141 taggttttct ttttttagtt atcaccattt taagtttttt taaatttttc ttgggaaatg
    52201 atgaaaattc tacaggtaaa ttagctttta accataaact acgaaaattt ttttgcagat
    52261 ctaataaaaa atcaaatttt tcgtttaata gattataaaa aatctcttta ggactattat
    52321 tgattgtcca caatttgtct atatatggat tatataaata tatatcttta taaattgctt
    52381 ttgttaataa atgaatttct atatcatcat atctctgatg caaacatcga atcataggag
    52441 ttgtgagtaa aatatcacct atagatgaaa aacgtacaac cagtatcttc ataagtattt
    52501 ttgcaaaaat ataatattta aaagaaatat gaaatttaat tttttttaaa tttcaatata
    52561 aacacagaat aaataaagcg ctatgataaa tgtaaaaaaa cgagaagtat ggcaagttgg
    52621 tatttgataa gtcaataaag tgtgaaaatt ttgaatattt aaattaatct tcggtatgag
    52681 aagaaggttt tacaatgttt tctctcattt cggtatcagc ttttatattt tgcatgcgat
    52741 agtaatccat aacacctaaa ttacccattc taaaagcttc tgctatagct aatggtattt
    52801 gagcttcagc ttctattact ttagctcttg cttcttgagc tttagctttc atttcttgtt
    52861 ctaaggctac agccatagca cgacgttctt ctgctttagc ttgagcaata tttttatcag
    52921 cttgagcttg atctatttgt agctgagcgc caatattttt acctacatct atatctgcta
    52981 tatctattga taatatttca aaggctgtac ctgcatctaa tccacgagtt aatacagttc
    53041 tagaaatcac atcaggattt tctaaaactg ctttatgatt ttcagcacta cctatagcag
    53101 ttactatacc ttcacctact ctagcaataa tagtttcttc accagcacca cctactaatt
    53161 gtttaatgtt agctctcaca gttactctag cagttactat aagctgaata ccgtctttag
    53221 ctacggctgc tatagctggt gtagttatta ccttaggatt tacactcatc tgtacggctt
    53281 cgaaaacatc acgtcctgct agatctatcg ccgctgcaga tttgaaatct aatggtatat
    53341 ttgctttatc tgctgagatg agagctttta caacagaatt gactctccca cccgctaaat
    53401 aatgtgcctc taactggtct ctggttagat ttataccagc ttttgtagca gttatcaagc
    53461 aatttactat cacttttgga ggcaccttcc tccatcgcat caatattaat tgcaataaat
    53521 ttatcctgac accactcaaa agagctgtaa accataatcc tattggcaca aaataaaaaa
    53581 tcaaccataa taatataaat attactatgc ctattataat atttgtgcct gtgtttaaaa
    53641 caattaaatt aagtgttaac atattttatt ttttaaaggg ttttacaatt attttgttgc
    53701 cgtctatcct tattatttca acctctgtat caggatatat catctctgag gtagaatgca
    53761 cttctacgat tttatcgcca aatcttgcct tgcctgcagg aatacattta gtaatagtat
    53821 gacctcgcat accaggtttt agcgtttcta tttcttcttt atttaacttt ttatctatag
    53881 tatcatttag agatattcgt tgccatgttt tttctctaaa gaaaaaatat aaacctacac
    53941 cacatactat taaagacact actaaagata tagtgccata tagattgcca tagatatcat
    54001 acattaaaat gatgcctgct atagtagcta taccaccgat agtacctact actccacccg
    54061 gtatcactaa ttctaaaagt aaaagaatta atccagctat tattaataag attatagtag
    54121 ttaaattcat ttctcgatct aataataaaa ttatttgcaa agatataaga ttatttgatt
    54181 aaatagaaat caattttagt atgttataat taattgaata gttgaattat attagcaagg
    54241 taaatttaat ttaagccctt tattttctta tattttaata tattaagcta taagtatgtt
    54301 agcaatattt aatttatcaa acataatatt ttgattttct ttaattattt atctaatacc
    54361 aatttatata aattactatc ttatattttt taatatcaag tctaaaaata tatcgattat
    54421 taacaattaa atattttaaa aaaatagaca aaatatgttg tactagatac aaaaataaaa
    54481 aacaaataga tatgataaaa agctatttga aatattagtt taataacaag agcatttctt
    54541 aatagcataa atcatcataa taagtatcaa aaaaaaatca gtatcttttc attttattta
    54601 ttaattttat gaatttataa aataaaaatg gattatggat aaaataaaat atttatggga
    54661 atatgatatc aaggtgcttt tagctctaga acaagctatc gctggagaaa aagaattttt
    54721 agatttttta cttactaatg gttatcctga gttaggagct ttagctaatg ctataagagc
    54781 tgatttagaa gcatatcaat ggcttattag atatggtttt ttagagttag caatgctttc
    54841 tgatgctata gatggagatg gggcggctta tgattggcta aataatcaag atttgtatct
    54901 atatatgatc attgcctcag catgtcaggg ttctgaaaaa gctaaaaatt atttagctaa
    54961 aaacaatctt gattatttta ttcgttttgc agatacagtg gctaacgagc tagaatggca
    55021 aagaagaaat caaactgatt atcataaatt tggaagttaa taagatatga gcgggacact
    55081 aggagaaaag agtaaaaagt attaattatt attagttaaa ataatctaaa gtatttttta
    55141 tcttattaaa atattataaa ataggagaaa aactgatagc tatgaaaggt aataaaaaac
    55201 ttaaaataaa tactaatgat aaaaatttag caaataattt ttgttgaaag ttacgaaatg
    55261 gaattaaaag taccgacaaa gaagataata aagataacca aagagtagga tatgtaatat
    55321 atctattatt agttacatta tatacacgtc gctgaggatt aatagaataa ctgcaaataa
    55381 tacgcaatag ccttgttcta ttcaaataaa taacaccatt tttttctaat aagtatatat
    55441 catttaacga gagacgttca gctaaaattt tatgattaca agctttaatt ataaaatctt
    55501 cctcatcact tataggttct attatatatg catcttgaca tttggtccat tgtctaggta
    55561 gataataatc aaataaaaaa aatacagtta atattaaaaa aatgatattt agccatgaag
    55621 gatataatat tttgttgata gatatattgt gtatttcctc ttcttttttt ttaaattctt
    55681 catatttcat acgagcataa gcttttgctt gctctctata aatatcttca tattgttggg
    55741 tagtttgatt agaagtagaa ttagtaatca aagtattttt atttaataaa aataaataag
    55801 cctcggtaat taaaataaat ttttctgcag aactttcagg agtattgcta tctggatgat
    55861 ataatttagc taattcttta tatcgctttt tgatatcatt ttcatttgca ttattcgata
    55921 atgacaatat attaagtgcc tcgtcttttg tcatatatac tttatatctt aattagacta
    55981 caaatttctt taaatgttta aattttgtaa tagtaataaa aaaatagtta aaaccaatat
    56041 tttttttgaa ttttatcaga tatgacaaaa aataactagg taagcatgtt ttcgattaaa
    56101 tatattcact acctttttct atcaaaatat cttgtacaat atttctaaga gattgttcgt
    56161 gatctttatt taatatcaga agcatatcat cattttctac gacaataaaa tttttcaatc
    56221 cttcgatgac tactagtttt ttgttattag gcatttgtat tagacaatct tgagtatcat
    56281 aagtcattac atttttacca ataatactat tattattttc atctttaggt cttttttcat
    56341 gtagagatgc ccatgtgcct aagtcattcc atggaaattc agaaataatg gtgtatacat
    56401 tattagcttt ttccattata gcataatcta ctgatatctt ttttatgctt gaatatattt
    56461 tttcaatttg ttttggttct ttttcagtat aataccaaga gagatctttc aaaaatgcat
    56521 tgtaaatatc aggttcatgt tgtttaaagg catcaataat agtttttgct ttccatacaa
    56581 agataccagc attccacaaa aaatctccac tacgaataaa tgttcttgcc aactctaacg
    56641 caggtttttc agtaaaagtt ttcactttac aaattttttg acctttttgt gggataaact
    56701 gtatataacc aaatcccgtg tctggccgag taggcttgat acctaaagtc atcaatacat
    56761 catgttgctt tacataatca attgcttgat ttagtgtttc agcaaacgca tcttcatcat
    56821 cgatcaagtg gtctgctggt gcaaatacta gtatagcatc aggatccttt ttaaaaattt
    56881 tagctgatgc ataagctata gcaggagctg tattccttcg atcaggctct acaattattt
    56941 gttcatcgct aatctcttgt atttgttctt ttaataaatc cacatgataa cttgagagta
    57001 caaatcttat atttttttta tctattacct tactatacct attatatgta gtttgtatta
    57061 acgtttgacc agtatgcaaa atatctaaaa attgtttagg tttgctctct ctgctcattg
    57121 gccaaaagcg tacaccagta cctcccgaca ttatgacgca gtaaacattt ttattcatag
    57181 ttattttttt gcaaatttaa gtatttttaa tctttaataa aaactaactt ataattatta
    57241 aattaatttt actttaaaca tttttatcta tatactatta aatttgttgc gacttatgta
    57301 aaaaatctaa aaataaaagt gaatatcaca atacaacttt atattacttt aataaacttt
    57361 atattacttt aataatagta tttcaatatt acattatgat tatactgatt taaagatttt
    57421 aatttttagt aatagtcaaa aaaaaaatat ggtaaaagtt ttgcactatt ttagttgaac
    57481 taaaatagtg caaagtggaa tcgaaagggg aatggaaagg agtccagaat caaatcattg
    57541 ttttacttga agtagtttat ctatatttcg tatttactag ccgccaatac tcatcccacg
    57601 cccatctatc cgaggatgcg aggtttaaaa gccgaccaat gtctgattct atctcacttg
    57661 tcaagtattt atgtgtgatt acaaaccaca tcagatagct ctgcaggtat ttagtagcca
    57721 ctcctctaaa tcgccgcaac caaaccttga aatcatttat atgcttatcg actacctgta
    57781 cactgtatac accgcgtcta gttatttaat tatgctataa actacttcaa gtaaaacaaa
    57841 ggatattttt aagtagctcc gttcacaaat cgtatttaca aaacgtaatc tttacaaagc
    57901 taaatttcct atccattttt atttgaatag agactaaaaa aattattgta aaggaatcga
    57961 aaaaagtcca gaaacaaatc attgttttac ttgaagtagt ttatctatac tttgtattca
    58021 ccag
//
LOCUS       DAOPRN010000006        53713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_460_length_53713_cov_129.277517, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000006 DAOPRN010000000
VERSION     DAOPRN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 53713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53713
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_460_length_53713_cov_129.277517"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKI65_01410"
     CDS             <1..581
                     /locus_tag="PKI65_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77130.1"
                     /translation="LPSILDLQILEINFVDSMSLWQPQVVIYNNSTRPVKKIQLDFGL
                     DYPKGFVYLWNGRLEMGGIETINLPFLLNIYELQSIFCVKAEAIDVVDQSDISIDNNT
                     LCATLSNKLFIVRLFSSDDYLNLWINLPYSGLLSCKIINTLGQIMFSSESIDLSRGIN
                     QVKIPIYNLTGAIYLLEINFEGKILMRLFNKI"
     gene            complement(690..2288)
                     /locus_tag="PKI65_01415"
     CDS             complement(690..2288)
                     /locus_tag="PKI65_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77131.1"
                     /translation="MKNFFSSFLLLIIVLSISAQNYQSLKIKEEFDFFNKIMIDSYYF
                     PHKRHYTDNFDVKREPHNIYTVSSFGDGMARILEGYITMYQITGDKAYLYKFVLQSLC
                     IIEERPDYSIDNTKQPRWAFDPFMYLDGQIIAALSKFAYFVKFEEPFLIEEPIYQFDE
                     LNPLKYKPNTCFCNRFDINFIKFGQYADWLIDRACETLDWYLTSGYWDNNLGFKEKPD
                     DIAAAQINMQIGFSRALLFIGLYNENIYYLDKAKIISSLFKSNIKFFDICKLKSYEAP
                     VFIQTSDNSYWWYHNGWRIPYHYCIIRSKSYSGYTEYIEDISHGINVTWLFLDFFKYQ
                     PNTPFTIDDLIRLRNMFTKHIYNGRFYNSVLGTDGPVFPKNNLIYTVLNFMPFSKYDG
                     ADSTAIPPNAYDIILQFYSNNISNNNKIPDTPYYNGGLSVKGHAEIVQAQWEHECTDL
                     ILYNRKVVYDQDFCAKGTLTIAPQQYDYLHKPNDESFAEPLITENVFTVEPGTTVNMS
                     ARKKIVLKAGTHIKQGSNFRAFLY"
     gene            complement(2285..3736)
                     /locus_tag="PKI65_01420"
     CDS             complement(2285..3736)
                     /locus_tag="PKI65_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOB77132.1"
                     /translation="MKNNISLENLDSLFPVSFNEEQLSRAKTIFFKKLSELSHRYYNG
                     KIQIAPKVPTPGFNWFNVWYTPGVSSISTLIRDNNDESFELSNRGNLVAVVSDSTRVL
                     GDGDVTPPGGLGVMEGKAFLMKYLGGIDAVPLCIDSRNEKGQNDPENIIKFVLMVQHS
                     FGAINLEDISQPNCFKVLDTLRESCDIPVWHDDAQGTACVTLAGLLNALKIVNKKIEN
                     VKITLYGAGASNTTIARLLIASGADPENIIIFDSKGSLNIDREDIRNDMRYYRKWELC
                     STTNPKKIKNIEDAMKGADVLISLSKPGPDTIKQEWIRLMADKAIVFACANPVPEIYP
                     YAAKEAGAFVVATGRGDFPNQVNNSLGFPGILKGALMVRSKKITDEMAIAAANTLADF
                     AYKRGIDVNNIMPTMEESEVFPIVAAAVAMEAIKNGVARKILSYQEAYDWAQKDISYA
                     RELTSAMIKENFISLPPEKLINEAIEYTINQIK"
     gene            complement(3750..4358)
                     /locus_tag="PKI65_01425"
     CDS             complement(3750..4358)
                     /locus_tag="PKI65_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOB77133.1"
                     /translation="MKKFIFLILLLISTFLTAQNFGGGFSIALNGSQVSGDQLSGFDK
                     FGVRTGVFTFYNISEQSKLKLELVFSQKGSRKNPTSEDPTSFLLKLNYVEIPLLFEYK
                     LPIKHLYVFVGLAGGILTKTKDNIWDENGLVVMLEPFYKFEFSCLVGFNFNFNESHQM
                     FFELDQSILPIRKHYSGATYYFNRGVYNTVITLGYAFTFPQK"
     gene            complement(4363..6489)
                     /locus_tag="PKI65_01430"
     CDS             complement(4363..6489)
                     /locus_tag="PKI65_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOB77134.1"
                     /translation="MKSSKVLLLILCLFVTQFINAQTINNYRDIFKYYQKFTPLMQVQ
                     AHPDGESFTLVDSLNNLVLFKNNKFSNLLSLDQLNQLLPPGLSNNQKTFPKYQWKTKD
                     TLIMTIDTTLYIYNFKTKKIEFINNWPQSAKNVTLEPTTLQVAYTIKNNLYIADNGIQ
                     SVVASDIDTNIVYGQSVHRNEFGINNGIFWSPTGKYLAYYRMDQRKVTDYPLVDVTTR
                     VATLKNIKYPMAGMQSHYVTLGIYDINKKSKIYMQTGDTLDHYLTSVTWHPSEKYIFI
                     GILNRDQNHLMMNMYDVNNGTMIKTLFEETNDRYVEPQTPLYFRPNNSDQFIYFSQRD
                     GYWHLYLYDISGKLIKQITQGKWIVNEFGGFLKGGEIVWFYGTYDSPIERHAYMVNLN
                     NLNFKKIDKGKGTHYVVVNQNGTACIDEYSNIDTAYHVQFIDFKKNKSTTIKMLLDFF
                     QKEILPKPDIFYLVNNNGDTLYSRLFLPLNFDSTKKYPIIVYVYGGPHAQLITDSWTG
                     GAGTFLPYLASKGYVVFTLDNRGSANRGFEFESIIYRNLGKYEVEDQMTGISWLKQKS
                     WVDTNRIGVHGWSYGGFMTLNLMLKHPEIFKVAVAGGAVVDWKYYEVMYGERYMDTPE
                     QNPDGYKNTSILNYVDNVHDKILLIHGYIDDVVVPQHVLSFLKACIDKGKLVDFFVYP
                     THSHNVKGVDRYHLNDLIENYFNENL"
     gene            complement(6740..7726)
                     /locus_tag="PKI65_01435"
     CDS             complement(6740..7726)
                     /locus_tag="PKI65_01435"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77135.1"
                     /translation="MDNFNLLDYQALPKEQKRRFLDNLYQFLLENNYTAVDYQKLKIQ
                     STAPICPRCESENVIKAGVRDGKQVYKCKDCGRQYRETARTFVYRMRKADKMLDYLKC
                     MLEGKSLRACASEVGISLRTSFNWRHKILAAIKSLQGGISFSGIVEADELLMKYSEKG
                     RKYRSRKEYEKAKKKKHPKVAVLVMTDREGNLLFKHVGRKKVTNSQIREELSHRVTDS
                     NLICVKQKKEFKKAVKGTKSKKVVVNKTQIRRGVYSVQVVDKHINDFKVWLRRFRGVA
                     TKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSDRWAWDEYWRLVNTKYR"
     gene            complement(7855..12903)
                     /locus_tag="PKI65_01440"
     CDS             complement(7855..12903)
                     /locus_tag="PKI65_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOB77136.1"
                     /translation="MKQFKYFYLIIFIITNKLFAQPFGNEWINYNKFYYKIKISSEGI
                     YRIDYNTLVSAGIPVNIIDPRLFQIFHNGIEQYIYVHGEEDGIFDNDDFIEFYAKGND
                     GSFDKKLYKNSEHQANDNYSLFNDTSAYFLTWSSSLNNKRVANYKNTEYLSYFPESHV
                     YYTNRIDYTSYYYPGPVDASGLSDFDYTEAEGWFDGTMSINPSQPGVPQNYNKQIQTP
                     LPYNAGGPSEIKIMITGQSSALDYPHHLKINFLSNNIDTTFIGYKNVFISRNFLSSSL
                     SSNLNINFTLPNDLGAPSDRIALSYIEVSYPRLTNYNNSYSLKYRIKDNGQNVRYIHF
                     INVYGNNNDTLISYDLTNNNRIFSTKNNNNVDICIAMNGNDVEIYSTIESNVKKITQL
                     TAINGTGKFTDYLSLGLIKADYLIITHPILINSANNYANYRSITGYSPLVINVEELYD
                     QFAYGIGKHPLGIRNFLLFAITKAEVPIKGVFLIGKPYKAGEGNYNYRKKPDIYAQTL
                     VPSIGNPPSDQLFLSGIVDTLYQPAIPIGRLAARNPNDVDVYLQKVIDFENEMKLPYN
                     PNLPIDQEWRKKVLHFAGGSNQVEMHQFLSYLNQYKNKIENPYFGGNVWTFTKTSTDP
                     IIQSLSDSIRNLVDRGVIMMNFFGHAAGLGFDISIDNPNTYNNYKRYPFVLANSCFVG
                     DLFQVDATTSEQFVLIPNKGMIGFLGTNTASYTSYLHLFSNQLINHLSIESYGKSIGE
                     IVKNTIRDIQVPNNFYNKVICYDFILHCDPVIRLSIDSLPDYMVTNQTIYTQPEIISN
                     EIDSFKFCVTAHNLGKAIYDSVFVEIIRTYPDNTTETYIKRIRTPFFKDTIIFTIPTN
                     PLISIGMNKFKIIIDSYNDILEKNENNNTTEYILWVNSYDAVPIYPYEFAVIPDTFVT
                     LIASSSNPFPLMNKYIFEIDTTIYFNSPFKLQSPIIEQTGGIIQWTLPFTMLQMPDSI
                     VYYWRVKTLTSSYWRNSSFQFIKNKRGWGQAHFFQFENNDFVYMTTHRPTRTFNYINT
                     IVNIKAQTGYYPYIPWHEEWYKMNNVLKGQWSCTGDGGHGMKFAVFDTISITPWANTD
                     PDNDGLGPYDALNCRNYIYYDFDFYTTDSIWWQRMTNFINAVPDGNYILAFSHRNHNA
                     ENYPEDLYQAFESFGSAMIRSLPNNRPYMIFGRKGYPIGTADEIIGTEISSIINHSWN
                     LTTKWKEGSMSSPLIGPALQWNSIHWRVRSLEDGPITDTVRLFVLGIKENGTIDTLFA
                     NIRPISDSLDIYNLDQQIPANTYPYLKLLMKSSDDAMHTAPQPIRWQVLYTPVPETAI
                     LSFTKEYEFYKDTLQEGEIGKLKISTKNISSQDFQDSLWVKYWIKTNINEEINITLRK
                     TRLHPANDVLTDSIKFSTIGLKGFNQLWVEFNPINPNTGDYFEIEQTHINNIASIPFY
                     VNTDKINPLLDVTFDGIHILNGDIVSAEPHIKITVRDENKFLILDDTSAVTIFLKKPN
                     ASEYERIYFVKSNTYQLIFTPSSDANSNVCYIDWYAKFEVDGIYELQIQAHDRSGNPS
                     GIIDYKIKFEIIQKPAITYLTNWPNPFSTKTHFVFTITGSHIPEYFTIQIMTISGKIV
                     KEITREELGHIHIGRNITSYTWDGRDMYGNLLANGVYFYRVVTRLHGDPIEHIQTELD
                     QYITKGFGKMVIIR"
     gene            complement(12900..13979)
                     /locus_tag="PKI65_01445"
     CDS             complement(12900..13979)
                     /locus_tag="PKI65_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77137.1"
                     /translation="MRQYISFIISLIISTILYSQVRSYTNEFLYLGIDARSMAMSNTA
                     IASTYNANAGYWNPALLVFAPAYPQFSLMHAEYFAGIANYNSFAISFHNKDSLAFAFS
                     YLRFAVDDIPNTTQLIDNNGQVHYDKITSFTAADNALLFSISKKINHTLQIGGTLKII
                     RRVIGDFAGAWGFGLDVGVSFMSDRWRGGLVVRDITSTPTFWSFSLNDKMKEVFTLTG
                     NEIPTNSLELGIPRLAMGICYVQPLLNNFLIQPELSLLFTFDGQRNTLISNKFFSIDP
                     SLGFEISFKQMIFLRGGIGSLQKENFPTGKKWTFQPNIGIGIAIKDILQIDYAYTDIG
                     DNSIALYSNIISLIYHIKKNSNTAL"
     gene            complement(14029..14619)
                     /gene="nadD"
                     /locus_tag="PKI65_01450"
     CDS             complement(14029..14619)
                     /gene="nadD"
                     /locus_tag="PKI65_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOB77138.1"
                     /translation="MIDQKNIGLFFGTFNPPHIGHTLIANYFYITNDFDEIWLIVSPQ
                     NPFKKNSNLLDANLRLKMVELAIEDAHFLKASDVEFDLPKPSYTYKTLETLTTRFPHY
                     NFSIIIGSDNAVSLDKWENYQYLIDNFTIYVYPRNGFLSSNIFKHKNIFIDSNAPYID
                     ICSKWIREQLSQNKDLRFFLRKSVYDFIIENKLYVS"
     gene            complement(14749..15204)
                     /gene="dtd"
                     /locus_tag="PKI65_01455"
     CDS             complement(14749..15204)
                     /gene="dtd"
                     /locus_tag="PKI65_01455"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOB77139.1"
                     /translation="MRAVIQRVSKASVKVGNQIIASISQGLLILIGFEELETKEDFKW
                     LTNKIINLRIFNDTKGLMNLSVSDIDGDILVVSQFTLHAWTKKGNRPSFARAAPFQVS
                     ENYYKQFIEILQNVFHKPIACGVFGAYMQVELINDGPVTIIIDTKNKNF"
     gene            complement(15204..16208)
                     /locus_tag="PKI65_01460"
     CDS             complement(15204..16208)
                     /locus_tag="PKI65_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOB77140.1"
                     /translation="MGNRRLIHILLCLLLIFCLILSESCKRKAKVTGEIEEKTEIIVP
                     DFNCDSAYTYVAKQVDFGPRVPGTQSHKLCGDWLVSMLDKYADTVYVQPFKARIYTGE
                     VKEGRNIFAQFNPDIQKRILLCAHWDSRPYADYDPDPKNHNLPIPGANDGASGVGVLL
                     ELARIIHKQNISIGIDIAFWDLEDWGEPQNVNNYGGEYWCLGSQYWCKNKVPMFYNAN
                     YGILLDMVGATDATFYFESISYQYASSILKKTWDIASKLGYSKYFIPDYSNPITDDHL
                     YLNTIAQIPTINIVHQDKTSPTGFFPYWHTMEDDIDKIDKNTLKVVGNVLTYLIYNEK
                     "
     gene            complement(16190..17662)
                     /gene="cysS"
                     /locus_tag="PKI65_01465"
     CDS             complement(16190..17662)
                     /gene="cysS"
                     /locus_tag="PKI65_01465"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOB77141.1"
                     /translation="MDHPLYLYNSLTRKLDRFVPINEPFVGLYVCGPTVYGDPHLGHA
                     RPAIIFDVLFRYLKELGYKVRYVRNITDVGHLENDADDGEDKIQKKARLEQLEPMEIV
                     NYYTRRYHQALDLLNIMPPSIEPFASGHIPEQIELIQEILDNGYAYLSNGSVYFDVIK
                     YQNDTNLYGILSGRKLEDLLAHTRELQGKEEKKSPYDFALWKKATPQHIMQWNSPWSK
                     GFPGWHLECSVMGAKYLGIPFDIHGGGIDLQFPHHECEIAQAVAAKGVQPVKYWVHNN
                     LITINGQKMSKSLNNFITLEELFAGNHPALENAFSPMVIRFFILQAHYRSPLDFSNEA
                     LKAAEGGLNRLTKAFMKIEKLSPNTDKTDLPLLNLKNNILSALNDDLNTPIAIANLFE
                     AVKHINLASEQKINLSSDDIEILYWMKNTIIENILGIKLESSQNVKDIAPFIDLILDI
                     RWQLKQQKNYALADDIRNRLIELGIKIYDLKDKYTWEIED"
     gene            18288..19238
                     /locus_tag="PKI65_01470"
     CDS             18288..19238
                     /locus_tag="PKI65_01470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOB77142.1"
                     /translation="MKKNKSYYQSLHWENLYLKPYKTTKRYISIVLGVIATALALGFI
                     FTLIAIYIFPSKEEEKKAYELAQLEKIYEEIQNNQKQIEILLDTLSIKNNIIFKNIFN
                     TDYIDEDKEMNTSKITSAKSLSDMLKIIDENLTKIKQKSNIQYYYYNFLFHYLPENIN
                     DLMKLPIRMPLKENYEIASGFGERIHPILKNTFFHTGIDFAAPLRTPVYATASGVVRN
                     PDKSMFGYGETIIIDHGNNISTVYAHLLEKKVATGRKVSAGELIGYVGNTGISFGIHL
                     HYEIRIKNNPVNPIQFFITLDPQIFYHYYLQAKIFNQALS"
     gene            19491..21266
                     /locus_tag="PKI65_01475"
     CDS             19491..21266
                     /locus_tag="PKI65_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOB77143.1"
                     /translation="MIKKKKRPTFDFLKIGYRAVINQAIIPAIIIGIITGLAAVLLKN
                     GVYNLTAWVQDITRVGHFSLLYFFLPIIGIILTVIFTDNIIKEKLDHGVTRVLQSITK
                     NKGDIHPHNIYSNIVACVITAAFGGSIGMEGPIVTTGAAIGSNTAKFFNYSYKYKLLF
                     ISAGMSAAIAAIFKAPIAGLIFSIEVLAMDITAGFLIPVVLSCVTATIVATFFLGQKA
                     EFYFAVFTNYNYSNTLFFFLLGIFAGLLSIYFLRTFSFVEKKLSKIKKKSFKILIGGG
                     LLGLLIYLFPPLYGEGYNSMESILSGKIEQLFSSSIIYPYFNSQVGFLILLIILFFMK
                     VIATALTTGAGGVGGVFAPALFTGAVGGTAFALMMNLIFNTSLNINNFTLVGMAGVMA
                     GVIQTPLAAIFLIAELTGGYNLFIPLIITATIAYGTTRLVEPYSIYTKPLAERGELLT
                     HDKEKNVFTLLDKDELIETEFYPLTVNQKLADLVDAIKNCDRNVFPVIDNSSNLVGLI
                     HFKDIKKIIFEPDKYDKVYIKDIMKKPEIVIDINESVDSIIEKVRAKNIWNIPVVDNG
                     KYKGFISKSSLLSAYQEVFDEFIFE"
     gene            21386..22507
                     /locus_tag="PKI65_01480"
     CDS             21386..22507
                     /locus_tag="PKI65_01480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB77144.1"
                     /translation="MMTIYLHNPLTISLLIVFFLLLLYYLVIFIRLAFYKKTVNKKYP
                     PVSIVIVAKEEGFNLKNHLPLICEQRYPNQFEVLVVDDNSTDNSEDIICELKYKYSNL
                     NYLRLDNKMFEIYKGKKFPLSIGIKNTKFDYLLLTDADCKPASDLWLMTMMEAYSDKT
                     EIVLGYGKYEKTKGFLNTLIRWDTFFHSVMYFSAAIWGFPYMGVGRNLSYKKELFFRV
                     KGFTNHMNLMTGDDDLFIKDAANKDNVSICIDHKAHTISKAPKSLNKYFIQKKRHFKS
                     SSYYKIVPQIYLTLIHCLQIAFPVLIILQIIIQKFYIFAISIFIIKAIIQVLVLNKIN
                     KNLKEKNLIWMAPILDFLNSIFLFSFFIISIFNKETKWK"
     gene            22526..23116
                     /locus_tag="PKI65_01485"
     CDS             22526..23116
                     /locus_tag="PKI65_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOB77145.1"
                     /translation="MMLKDKKKINELVINAKNGDSMAFATLMNNYKDGLYFFIIKMVG
                     DRVAAEDILMETFYKAFTNLSLYSFDYAFSTWLYTIAKNNTIDYIRNCKKNVLHNCKK
                     DFINDDASSFLNDIAEDKKHEPDNIVIRQEEKEAVRDYVSMLKPHYRILVEKFYFEDK
                     KYEEIAEELNLPLGTVKGKLHRARKMINKFIGQNNI"
     gene            23248..27483
                     /gene="glgP"
                     /locus_tag="PKI65_01490"
     CDS             23248..27483
                     /gene="glgP"
                     /locus_tag="PKI65_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOB77146.1"
                     /translation="MEEKKLMPNFLFEVSWEVCNKVGGIFTVLSTKAYKLVDLLGSQY
                     ILIGPDIVKDAANGYMFEPDEGLYHKWVLKAREDGLRIRIGRWKIKGSPITILVDFRH
                     LFEQRNKIFTDLWLKYKLDSLYGGWDYIEPALFGYEAGRVIHHFYEYHITAQDKIVAN
                     FHEWLTGAGILYLEDKVPQVGTAFTTHATIMGRTIAGNGLPLYSEMTNYDPQHMAQKF
                     NIISKFSMEYCAARCADAFSTVSPVTAKECKYFLDKEPNVITPNSFDIDLVPQGDEYE
                     KIKAASREKIIRLFKATSGAEDPNPFLILLSGRYEMRNKGIDLFIKSLGKIKNQNPNR
                     TIYACIAVPAGIQGPIHDVLDAYNNNSVAIKKYLTSHYLSNEDHDPITNAFKAEGLIN
                     QDDNPVKVLFIPSYLDGHDGLLNIPYYEFLMGFDLTLFPSYYEPWGYTPMESTAYGIP
                     TLSTSLSGYGNWVKSLNIDTSQYIRIIERNDYNDDDVVKNIVSYVFEQLQLSEEQRKS
                     LRDKCWQVAKLAHWNNFICNYFDLYDSAIRESEKRLDLYYFKTIKDYVVTSPKEIEIA
                     EWRKVYVKPEYPASLLPLVDMIQNLWWTWDEEAIELLKNINPVYWIKSENNPIAMLEM
                     MSYEEIINLSKDQDFIEKLNSIYKRFTDYMSISPYKENDKLVAYLCMEYGIHAFLKIY
                     SGGLGILAGDYLKEASDSNFPIVAFGLLFRYGYFKQKLSRLGEQIVQYIPQKFTNLPI
                     TAVRNNDGQWLKIHLPLPGRNVYAKIWQVKVGRIALYLLDTDIEENLDEDKEITARLY
                     DAEWEMRLKQEYLLGFGSIDAMRAMGIKPTVFHLNEGHAAFANIARLRYYIKEKHFSM
                     QHALELVKKTSIFTTHTPIPAGHDKFSEDLMRTYFAHIPESLDITWEEFMDFGREHRL
                     ETKFSVTHLAIKTSTYVNAVSKIHKRVTCSMFKDLYKGFFESELFFDYVTNAVHPKTW
                     MTSDWQKLFLDCAGSDFFEHMHENHNYWKFIDNLKPATIWNLKLKQKNELYDSIIERL
                     GIEMPQRQESPTQIIRTLEELNKTPEILTFGFARRFATYKRAHLLFINLKRLADIVNN
                     PHYPVRFIFSGKAHPSDKAGEDLIKRIIEVSRMPEFIGKIIFIENYDTELAKLLVKGV
                     DVWLNTPTRPLEASGTSGMKAVMNGTLNFSVLDGWWAEGYVPGAGWALRQENTYDDTK
                     LQDELDAEIIYSIIEDEIAPTFYDRDNIGIPQKWVEMMKNAYFYIAPHFITKRMLLEY
                     DDKFYDNLNNYYYTLTNDDHKVLFEFISWKRKVLRNWDEIHIIDMEMYDSSKGPLPLG
                     EKFTAKLVMDLKELKPDDVVIELIFGKKVEGRIVDIEFTRTFDFIGCDDTKTIYECSM
                     PMEQSGVYNFSIRMRPNHPLLAHKQDFYLVRWL"
     gene            27751..31065
                     /locus_tag="PKI65_01495"
     CDS             27751..31065
                     /locus_tag="PKI65_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77147.1"
                     /translation="MYKYCVFIILFLIHINIFSQNIIDTTFIIHSYQDTIDINNSEIL
                     YIKLLIDTNKLINNQILDIENQKIYINIDTSIHLPFTYRCRLRYIKKKISTTLLSIIY
                     KDTLQSEELSPFHVIVPEIHPEERGEIYTNGYISRSIMMSSRGQSSVQSALDLQFYGE
                     IAPFQIEGNLNDKNLPVQPEGTSARLQEIDRVYITLKKDNFKFTLGDFNIHSPENAVL
                     SKYSRKLQGTSFSNISKTEKHSFNYGGVVAINKGKFSRIELKPIDGVSGPYKLQTIEA
                     NYIQVIAGSEKVYLDGILLKRGYDADYVIDYNTAEITFMPKIFIRTSMHIVIEFLYTD
                     YNYAQASYGLYTEISGDKWQWGINYFSEGDIKAQPLLLSLSDSDKVILAEAGANVPVK
                     IPSFRATNYKDQNITYAIIDTLDYDSVFVYSRDSTTQLYKVDFTYVGKNKGNYIVSNY
                     AINGKIYEWIAPINGIPQGEYEPIRILNAPKRINLIESYFFYKPSENLETRITIGYSQ
                     NDINTFSEQDNKSQGLAYKFELIGKPSSEHTLTFFSQGVQSQFTSLEPLYNPEENKIF
                     NRELYTSEKMELWNGLIYNFKNEKNNFAHIKTSFFNKSKNEYSIIANANARYYWQKFH
                     FVTTNDYSHTHLPSYQIDYLKHSSNIGFNIKTLWLGIDNKIEYHPIYKNKIMLNNSQE
                     YYSFSPYIGWGDSTQTFLKLYYEYYNEKMPLIRKQHKTGYQLEIKKIIWSLRQNNYFV
                     MQTSDTVKKNYFTSDNEVNLYTKNKSFNMQLRYILSQRQSPRIDYTYIEVPIGQGSHY
                     WIDANNNGLQELDEFHIANFNDQAQYILLTIPTQDYISSYHQTCSFNFRFSPQLIKKF
                     PVFLKNFSLQSFFDINQNTINFNFLGINKKPDSLINSSERFRSILEYHPQKINLSVRY
                     EFYKQKILQTLIVGNIEQKKLNHSLQFYIPIAKKWNLQSISEYTYQSNKSVFRSSTSY
                     SFAGYTEKLELQYLETKYQIRVGPRYSYVFDKEQNIKNNKIELYIEPRIISIKAGTFM
                     GKFGWIYNIAKNLSDPTMEYLMLEGYKQGNNGLIQIQWQRTLNKLIQISLQYEGRIMQ
                     HSSMTHVGSITVRALL"
     gene            31313..32983
                     /gene="rho"
                     /locus_tag="PKI65_01500"
     CDS             31313..32983
                     /gene="rho"
                     /locus_tag="PKI65_01500"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOB77148.1"
                     /translation="MDLNDLKFNQLVLIAKNLKISNPRKYKKSELISLIKDNPDFKEK
                     DILKIIEQLNENEKDKESNFTENNDEQNTKTKRKRIKTTKVDINEDTGKSTQPKASNV
                     SKKEKQHTQSVDNKKTDKDNKPIEESKIIDTDALEISDLADDEMVIDKLYKDEATDIE
                     KNVDSEMEIAEESNTKTKKIYFWENEGIIEVEGVLDILPEGFGFLRSSDFHYYNSPDD
                     VYVSNTLIRSLGLKLGDTIEGTIRPPREKEKYFPLVKVLKVNGLEPQYVKDRVPFDYM
                     TPLFPYKKFNLTGHPHETLTSRIIDLFCPIGKGQRGLIVSQPKTGKTMILKEIANAIA
                     YNHPEAYLIILLIDERPEEVTDMIRSVKAEVIASTFDEPAEKQVKISQLVLEKAKRMV
                     ECGHDVVILLDSITRLARAHNTMAPPSGKILSGGVESNALQKPKRFFGAARQIENGGS
                     LTIIATALIDTGSKMDEVIFEEFKGTGNMELQLDRKLANKRIFPAIDIVASSTRRDDL
                     LVPKDILQRVWILRNHLADMNTQEAMLFIQERMRYTQNNEEFLLTMNG"
     gene            complement(33026..34744)
                     /locus_tag="PKI65_01505"
     CDS             complement(33026..34744)
                     /locus_tag="PKI65_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOB77149.1"
                     /translation="MENFFLDNKSLKFHLNHHLMEKIVRIKEDDFSEKDIYDYAPQDV
                     EDAIDNYEKVLEIIGEIAGEVIANNAEIVDAEGPQLINNEVHYHPATQENYNSLMEAD
                     VMGMTLPRKYQGLNFPILPFVMSNEIIARADASFSNIWGLQDCAETINEFASEEIKDQ
                     YLPLFSQGKTGAMALTEPDAGSDLQAVQLKATYDEKKEKWYLNGVKRFITNGDADISL
                     VLARSEEGTTDGRGLSLFLYDRSDKAVTVRRIENKMGIHGSPTCELVFKNAPARLIGQ
                     RKLGLIKYVMSLMNAARLGVAAQSVGIAEAAYREGYKYALERYQFGKPIIQFPAVYEM
                     LNLMKAKLDGARTLMYETTRFVDIYKGLNYLSEKRPLNQNERDELKKYQKLSEYYTPL
                     VKLFASEYANQVTYDAMQIHGGSGYMKDYPIERLYRDARITNIYEGTSQLQVVAAIRG
                     ITNNTFREKLNDYNSQITHPELQNLKSSLIKMTSQYDHVYSKIKDTADQELLDFHARR
                     LVEMMGNIIISYLLLLDTQRDIQYLSSAQTFIKYANAQNTQHAGLITTMDDEDVCACK
                     NNFGIE"
     gene            complement(34752..35774)
                     /locus_tag="PKI65_01510"
     CDS             complement(34752..35774)
                     /locus_tag="PKI65_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOB77150.1"
                     /translation="MNSIFVYCELTEEKNIADVSLELCSKGRKLANQLDVQLEAIVIG
                     DVDEGIASQLSPYGVDVVHLAQDNRLFPYQTLPHAAIMIDLIKNEKPQIAIIGATPIG
                     RDLAPRVASALRCGLTADCTELAIGSHVEPKTQKEYKNLLLQIRPAFGGNIIATIINP
                     ETRPQMATVREGVMKKEIVSTTYNTKISKVDIGKILKPEHFILQIIERHVEAQKINIK
                     NANIIVAGGYGMGSKENFQMLFEFAKLIGAEVGASRAAVDAGFTESERQIGQTGVTVR
                     PKLYIACGISGAVQHRAGMAESATIISINTDPKAPINEIADYVIIGDVNSVIPKMIKF
                     YKQNIK"
     gene            complement(35798..36670)
                     /locus_tag="PKI65_01515"
     CDS             complement(35798..36670)
                     /locus_tag="PKI65_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOB77151.1"
                     /translation="MSFKIVVLAKQVPDTANVTQDAMKEDGTVNRAVLPVIFNPDDLH
                     ALEMALSVKDKIKGSEVILLTMGPVRATEIIREALFRGADRGYLLTDRRFAGSDTLAT
                     SYALSMAIKKLWPVDLVFTGRQAIDGDTAQVGPQTAEKLGLSQITYAEKLIDIDDSSI
                     TIKRRLGRGIEIIKAPLPCLITVNQSAPRCRFRNAKLVMKYKRAASISELQERNEDYY
                     QLFENKPYLKIDEWGVEDINADVNWLGLSGSPTKVKKIENVVFKAKENLILSDNDNDI
                     DNLMKTLINSHIIG"
     gene            36810..39095
                     /locus_tag="PKI65_01520"
     CDS             36810..39095
                     /locus_tag="PKI65_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOB77152.1"
                     /translation="MKKNFFVLFFLFTIKLFSQIDALHYNIYIDSIDFDDQLIFCKTT
                     ITLDVGINEISEISFDLLSLEIDSVKCENTLCLYNYNDSTLNISGLFVDTVDITIFYH
                     GHPTLDIGGWGGFYFSGDYAFNMGIGIDNIPHNMGRSWFPCIDNFTDKATYDYYITTE
                     ISKKAVCGGILIDSTFINLSTIQWHWHNPHPIPAYLASVSVGPYVVWKDTVQGINNIV
                     PIEIYVPNSSLAKVPGSFQNLKSIFHIFEKYYGPYKWSRIGFVGVPFSGGAMEHAMNI
                     AYPIACINGNLAYESLYAHELSHHWFGNLATCESAEEMWINEGWARFSEILYKEKLYG
                     KEEANLLIKNLLSDVLKKAHKVDGGFYPLCDIPQNITYGTTVYDKGALIVNTLRNYLG
                     DSLFFGATKYFLEQFAWSNASCIDIQESFEQYTNIVLNDFFQNYLYHSGTPHYRIDSF
                     NVSHNNEINFIQLYTSQHRYGGDYLTEHDSIEVWLWGANKEMDSLKINTGLQNYFIGF
                     HHAIAPIDVWIDPKQKTADAIINGYAFLKSISNHSIPNTNVIITPTYLEDSLLVFVEH
                     HFIGPHQILKNHPIHRISPNHYWTIHLPYLDNNKVNLKISYNRSYPINIDPSDFYLLP
                     TAASVDSLVLVYRKNSTEPWQIVNFTKTGNQVAGYLTTVNAKAGDYAFAISEPGQSNI
                     NDFDNTNYLQIYPNPTNDEFKIIVPTSGFIQIYSMIGDLIFEELIETEITYKILDKGT
                     FLVRFIPCNNTKTFQKLLFVY"
     gene            39235..40215
                     /locus_tag="PKI65_01525"
     CDS             39235..40215
                     /locus_tag="PKI65_01525"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOB77153.1"
                     /translation="MKKKLIILIIIISFNNLSGQITELEKELKKKNKDSLNGWSFNGL
                     ASINFSQTALINWASGGENSISGHGIFNINANYLKNKNSWDNSLDLMYGMFIEGKKKL
                     KKTDDKIELFSKYGREFSKNLYISSLLDFKTQMTPGYNYPNDSLIISDFMSPAYLYLA
                     AGIDYKPFDFLTAFISPITGKFTFVYNQSLANNGAFGVEPAIYDEHGNLLKKGKNSRS
                     EIGGYIRLAFDKEILTNVTLKSKIDLFSNYLYKPQNIDIDWELLISMKINKYITVSIS
                     TRLIYDDDVMIEIDSNNDGIVDKKGPRIQFKEIFGIGFSYKFDKKRVSVD"
     gene            complement(40368..41879)
                     /locus_tag="PKI65_01530"
     CDS             complement(40368..41879)
                     /locus_tag="PKI65_01530"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOB77154.1"
                     /translation="MYFEELIPLQDFIDKDKQDYQVEDIVKYVKKFDINHLNFHYIGD
                     DGRVKTLNFIIQGEDHLREILSTGERVDGSSLFKYLETGSSDLYVIPKLHSAFLNPLE
                     VDPTLGFFCKYFDKDGNPTLFSPENILIKAEESLVKETGIEMQGMGELEYYVIFPKDP
                     LYLATDQRGYHESVPFVKFENLRREALNCLKIMGASIKYAHSEVGNFSDDTYSYEQNE
                     IEFQPVALEDAAIQLIIAKWVLRVLGYKYGVQVSFAPKITVGKAGSGMHIHMKLLKNG
                     ENVVFNQNGEVSDISKRAIAGIMELAPSLTAFGNCVPTSYLRLEPNQEAPTKICWGER
                     NRSALVRVPLGWSKNAVEMFKNTNYGKDPEKVNISQKPTFEFRVPDGSADIYLLIAGL
                     TVAVREGLNNPNALDIADKYKIEGNIFKEEYKDIAKKLPNLPTSCVESSKRLKEQKDI
                     YNKFDVFPAKVIEHQISKLESYNDENLSQKIADTPEPERTKMIKQLVYKYIDC"
     gene            42104..43228
                     /locus_tag="PKI65_01535"
     CDS             42104..43228
                     /locus_tag="PKI65_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOB77155.1"
                     /translation="MNAKYLFLIFLNLSLLNFSISAQILVKIGIRNDLKNIKIKISEG
                     DYALTNGNDSIILKKNDTIILENNYNKLSARTGIKNIKGDSILLISTSTNGIFSTIIN
                     NKLIEYEDNAIFYPKNNTSINFINQLDIDKYVASVILSETYPNLPYEFYKAKSIVVRT
                     YTTYMINIEKKHINDKYDLCCTTHCQLYKGKCNNQTIISACKETKGMILVDNDNLPIL
                     AVYHSNSGGLTNSSENYWGKSLPYLITIKDEYSTKSKNSTWTYTISKDQWLSYLAKHG
                     IDSTNKNFENATNFYQPIRKKYFIDTLTLRKIREDFNLKSTYFNIQDEGDKVIFKGRG
                     YGHGVGLSQEGAIEMAKEGKNFNEILEFYYPNTKISYFNL"
     gene            43234..45189
                     /locus_tag="PKI65_01540"
     CDS             43234..45189
                     /locus_tag="PKI65_01540"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOB77156.1"
                     /translation="MNWAKAPIVRITLFYIIGIIIYNHLPSHNFFYIVTLLTTFLLVL
                     SFGFYFYYSNKISLHTWYLALTIFLSFIALGYFRSYTFDERTKEDFYANHLDKTSYYF
                     GDITDIKASSQTRTKATVNLKKMLIDSQWVNTNGKVLIYLNLPIDSLQDNDHSIIFIN
                     SLQSPNLPKYPYEFNYAQYLANQQVYHTGFINDYDFFLLPRESTFLEQLSRVRNKIVE
                     IIYTNISDPASANLIAAMLLGYKDAMDAEIIKDFQQAGVVHLLALSGLHVGIIYGGIY
                     LLLSFIIPFKYRNKITIPLALITLWLYTLMVGAMPSVVRAAIMLTLLQIGVLFNRPVN
                     NYNSLATAALFILIANPLQLYSLGFQLSFTAVLGIFFFNAPLMNICKTNKKWLKWVIG
                     SLSVTMAAQLGTLPLTIYYFHQFPTYFLIANLIAVPMASILIYIGFFAIILSFIPFVG
                     LLIKKIFALLVKLFILFIRFISDLPYSTLSNIYIDPLSAFLIGIIIILIVLQIYYKNI
                     KIYPSLIITVILFGINYNLSIAKRIRENPSFEILNNKYAVIGQKEQEKFHYLIIKPDS
                     AQLPDINTKIDMFCNYYYCKNNNSYDLQDKVKSSAFYLNNPVIANKDQTFVVGDKKEI
                     LHWKELTKLPYIEIGWYRLRYYDIIDE"
     gene            45347..46609
                     /gene="rodA"
                     /locus_tag="PKI65_01545"
     CDS             45347..46609
                     /gene="rodA"
                     /locus_tag="PKI65_01545"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOB77157.1"
                     /translation="MQKNIKRRFPPDFTLIFIYLILSIIGLVNIYSASYQDGISFFSF
                     SFQYVHVKQFVWILTGWILFILIIYSNPHLFSNAAEIFYAIVILLLFLVLVIGAIVGG
                     NKAWLDLGKGIRLQPSEFAKYTTALLISKIWSYPDAKININNTIRKVLLVIFIPILLV
                     FFQKDVGTMLVFFAFIFALYREGLPTYVIFIGFWFIIVFILSFLVKQFTFLLILTGLT
                     LALIFIFRKKKNIIIKFVQVYVLSIVLIIGVNYFYNMLMPHHQERIEVMLGLKDDPKG
                     ASWNLRQSMIAIGSGGFTGKGFLQGTQTQMKFVPEQTTDFIFCAIGEEWGFVGAFVVI
                     ALYLFFLLRIIILAERQRSIFSRVYGYAIASIFFTHIVINIGMTVGLIPVIGIPLPFL
                     SYGGSSYWAFTLLLATFINLDMYHNEIV"
     gene            complement(46729..47313)
                     /locus_tag="PKI65_01550"
     CDS             complement(46729..47313)
                     /locus_tag="PKI65_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOB77158.1"
                     /translation="MKETIKNLVKAFIGESQARNRYTFYAKIAKNEGYEQISEIFTMT
                     ADNEREHASWLFKMINELKQETNENIDEITVEANAITILGSTIDNLKSAIAGEEYEYN
                     SMYPEYANIADKEGYSAIAIRLRAIAVAEKHHAERYAKILNQVEAGTFFKKDIPVSWF
                     CRECGYVHVGTEPPQVCPACSHPRSFYQLKVEEY"
     gene            complement(47338..47910)
                     /gene="ruvC"
                     /locus_tag="PKI65_01555"
     CDS             complement(47338..47910)
                     /gene="ruvC"
                     /locus_tag="PKI65_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOB77159.1"
                     /translation="MSLNDYYKILGIDPGSNVTGYAIIGIDDDKLITLDIGCISVKSV
                     KDHFERIKLIHRNVKELIISYNPNVLAIEAPYYGKNVQSMLKLGRAQGVVIAAALEYG
                     IDVYEYAPRRVKISVTGNGRASKEQVAGMVNNILKLENNSLSFDATDAAAIALCHYYS
                     TYSSIIPENMMNGKKNKWLKFIEENPDRII"
     gene            complement(48003..49388)
                     /locus_tag="PKI65_01560"
     CDS             complement(48003..49388)
                     /locus_tag="PKI65_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOB77160.1"
                     /translation="MAKMLDLTKGDERKTIVKYAVPLIVGSVLQNLYSLINSVIVGNY
                     LGKEALAAVGISFPIIFAVTSLIIGFTIGLSIVIGQYFGSKQYDNLKKSISTSYISLV
                     VFAIIMTTFSILTGKYLLKLLGAQEDIIAQATSYFNVFCLGFLFAFPFNATNAVLRGI
                     GDSITPVYFLVFSIVVNFVLNYVFVVVLNYGLTSTAWASVIGQILALILMIVYINKKH
                     PFISIKLKEMKFDSKIFKYIMKISLPSGIQQFVIAINAVVLMSLVASFGTDAIAGYSV
                     ATRIDAIASIPAFIFSSALANFVAQNAGALEFKRIKNGLKATLRITVSMTIIISVLII
                     IFSKTIMSLFTKDIAVIEIGSEYLRTLNYFYVLYAMMFAYQSVFRGSGDTMISMLITL
                     AAIWIVRIPFSYMLSKHLGTLGIWLGIPIGWFVGLILSYSYYKSRRWELKFVMQMRTR
                     HTPPPEIADNI"
     gene            complement(49449..50030)
                     /gene="pth"
                     /locus_tag="PKI65_01565"
     CDS             complement(49449..50030)
                     /gene="pth"
                     /locus_tag="PKI65_01565"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOB77161.1"
                     /translation="MRDNVFLLVGLGNIGYEYEHTRHNVGFDVLDYFVKKYSSAFKQD
                     RYAYRADIKIKNKFFICIKPTTYMNLSGRAVDYWVKMLEIPLDNTMIILDDIELPLGK
                     IRIKAKGSAGSHKGLANIIDVLHTTQIPRMRIGIDDKFTPGKQIDYVLEKWPLREWEI
                     INKVFPIASDAIYTWAFEGIDKAMNEYNNLVIT"
     gene            complement(50045..50467)
                     /locus_tag="PKI65_01570"
     CDS             complement(50045..50467)
                     /locus_tag="PKI65_01570"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77162.1"
                     /translation="MKILTKFIFVFITIISFTGCKKDLTQVIEEFYPDGNPKIVYYLD
                     KNNNKVKVEEYYPNGKLKYVGGLNNGLRHGIWKYYHPNGKLCSEGEYNNGTREGKSLV
                     YFENGNLRIKGSYKGGKPYGKWYFYDENGKLIETKDFH"
     gene            complement(50464..51144)
                     /locus_tag="PKI65_01575"
     CDS             complement(50464..51144)
                     /locus_tag="PKI65_01575"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOB77163.1"
                     /translation="MEEEKNLDPNYESSTPSESIDSNLTNNNAYTKKYNNFFIWWCVI
                     LSILVLFLLIIQFVPTKKISAPVSTGYVNGKIAHINIDTIMTKYPLVDTLQNRLQRQI
                     TSLETDLINRQSRLQKNMEAYQKDIQAGKIITKDEAGRREEQLVKEQQQLMSIRDQYD
                     IQIQNLQMTMQSEILDSIKNAIKYLPAELEFDYVLGYSHEGAIFYANPSLDITNEVLK
                     ILNKRLKK"
     gene            complement(51187..53415)
                     /gene="recQ"
                     /locus_tag="PKI65_01580"
     CDS             complement(51187..53415)
                     /gene="recQ"
                     /locus_tag="PKI65_01580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOB77164.1"
                     /translation="MNAETIGTSNITREEIYYILKSVFGYDNFKLYQEQIICSLLAGR
                     DTFVVMPTGGGKSLTYQLPALLCDGTAIIISPLLALMKNQVDIIRNIHTDDKVAHMLN
                     STLPRRELKQIFDDLANGKTKMLFIAPESLKKESYIDFFKNLKISFYAVDEAHCISEW
                     GHDFRPEYRMIRPMIESIGQDVPVISLTATATPKVQEDIIKNLKLKNPNIFISSFNRP
                     NLYYEVRPKTKNINKELIQIIREREGKSGIIYCMMRKTTEKIAEFLQANGFNALPYHA
                     GLSGAIRRQTQDLFLRQEIDIIVATIAFGMGIDKPDIRYVIHYDMPKSLEAYYQETGR
                     AGRDGGEGDCIAFYNEDDLIRFDKMEQNNRGVAEKEIAGLIMYETISYAESTICRRKL
                     LLHYFGEDYTEDNCHCCDNCLYPKMQFDAKNEVLTVLNVIDETKELYKAKMIVKILLG
                     KEKDKATLKQFKAEKLKSYGAGADYDEKFWNSIIRQILILNFLEKDINNYGVLKITEK
                     GREFLTKPKSIMLTRDRDFSEGNEELDDEIKMKGGSGADAILLKMLKDLRNKIAKQHN
                     LPPFVIFQDPSLEEMTIQYPITLDELQNITGVGVGKAQKYGQPFVDLIKRYVEENEIE
                     RPNDIIIRTSPNASNLKIFIIQSIDRKIPLDEIAYAKNLNFDELLDQIDKIVASGIRI
                     DIDYFIDDVIDPYHQEEIYEYFRSASTDDIKQALEELGENEYSEEEVRLMRIKFISEM
                     GN"
BASE COUNT        18724 a         8203 c         7691 g        19095 t
ORIGIN      
        1 tattgccatc aatactagat ctacagattt tagaaattaa ttttgtagat agtatgtctt
       61 tatggcaacc acaagtagtt atttacaata atagtactag acctgtgaaa aagatacaat
      121 tagattttgg acttgattat cctaaaggtt ttgtatactt atggaatgga aggttagaaa
      181 tgggtggaat agaaacaata aatttacctt ttttattaaa tatttatgag ctacaatcta
      241 ttttttgtgt aaaagcagaa gctatagatg tggtagacca gtcagatatc agtatagaca
      301 acaatactct gtgcgctacg ttatctaata aactatttat agtacgactt tttagtagtg
      361 atgattatct aaatttatgg attaatttgc cctatagcgg tttattatct tgtaaaataa
      421 ttaatacatt aggacaaatt atgtttagct ctgaatcaat agatctatcg cgaggcatta
      481 atcaggtaaa aataccaatt tataatttaa caggagcaat atatttgtta gaaatcaact
      541 ttgaaggtaa aattttgatg agattgttta ataaaatcta atgatagtat atggaatagt
      601 gtgaaacaaa aagtagcaga gaggatgaga agagctgaaa atgaaaaatt aaaaagttta
      661 gaaaaatgtt attaggtatg tgagattaat taatatagaa aagctctaaa attacttcct
      721 tgcttaatat gggtacctgc tttcaaaaca attttttttc tagcagacat atttactgta
      781 gttcctggct ctactgtaaa tacattttct gtaattaatg gttcagcaaa tgattcatca
      841 tttggtttat gtagataatc atattgctgt ggtgctattg taagtgtacc tttagcacaa
      901 aaatcctgat cgtacacaac ctttctatta tacaatatta gatctgtgca ttcatgctcc
      961 cattgtgctt gtactatttc tgcatgtcct ttgactgata atcctccatt ataatatggg
     1021 gtatctggta ttttattatt atttgaaatg ttattagaat aaaattgcaa aattatatca
     1081 tacgcattag gcgggatagc tgtagaatct gctccatcat atttagaaaa gggcataaaa
     1141 tttaatacag tgtatattaa attattttta ggaaaaacgg ggccatctgt acctagaaca
     1201 gaattataaa atctaccgtt ataaatatgt ttagtaaaca tatttcgtaa tcttattaaa
     1261 tcatcaatag taaaaggagt attaggttga tatttaaaaa aatctaaaaa taaccaagta
     1321 acattgattc cgtgacttat atcttctatg tactcggtat agccgctata acttttgcta
     1381 cgaattatac aataatgata tgggatacgc catccattat gataccacca ataagaatta
     1441 tcacttgttt gaataaacac cggagcttca taagatttta atttgcaaat atcaaaaaac
     1501 ttaatattac tcttaaataa agatgatata attttagctt tatcaagata atatatattt
     1561 tcattatata atccaataaa caataaagct ctgctaaaac ctatttgcat attaatttgt
     1621 gcagcagcta tatcatcagg tttttcttta aatccgagat tattatccca ataaccactt
     1681 gttaaatacc agtctagagt ttcacaagct ctatctatta accaatcagc atattgacca
     1741 aatttgataa aatttatatc aaatctatta caaaaacaag tattaggttt gtatttaaga
     1801 ggatttaatt catcaaattg ataaattggt tcttcaatta aaaaaggttc ttcaaattta
     1861 acaaaataag caaatttaga taatgctgca ataatctgtc catctaagta cataaaaggg
     1921 tcaaaagccc atctaggctg cttggtatta tctatactat agtcagggcg ttcttcgata
     1981 atacataatg attgtagtac aaatttataa agatatgctt tgtctccagt aatttgatac
     2041 atagtaatgt atccttctaa tattctcgcc ataccatccc caaatgaaga aactgtataa
     2101 atattatgag gttcacgttt aacatcaaaa ttgtctgtat aatgcctttt gtgagggaaa
     2161 taatatgaat caatcatgat tttattaaaa aaatcaaact cttcttttat ttttaatgat
     2221 tgataatttt gagctgatat agataaaact ataataagta ataaaaagga tgaaaaaaaa
     2281 tttttcactt tatttggttt atcgtatatt caatagcttc atttataagc ttttcaggtg
     2341 gtaaggaaat gaaattttct tttatcatgg cagatgttaa ttcacgagca taagatatgt
     2401 ctttttgtgc ccaatcataa gcttcctggt aagataaaat ttttctagct acaccatttt
     2461 taatagcttc catagctact gcagctgcta ctattggaaa aacttcagac tcttccattg
     2521 taggcataat gttattaaca tctatacctc ttttataagc aaaatccgct aatgtattgg
     2581 ctgcagctat agccatctca tcagtaattt ttttagatct taccattaga gcacctttta
     2641 atattccagg aaaaccaaga gaattattta cttgatttgg aaaatcacct ctaccagtag
     2701 ctactacaaa agcaccagct tctttagctg cgtaaggata aatttctggc actggattag
     2761 cgcaggcaaa aactatcgct ttgtctgcca ttaatcttat ccattcttgt ttgatagtat
     2821 cgggtcccgg tttagataga gatattaaaa catctgcccc cttcatcgca tcttcaatat
     2881 tttttatttt ttttggatta gtagtactac agagctccca ttttctataa taacgcatat
     2941 catttctaat atcttctcta tctatattta atgaaccttt gctatcaaag atgatgatgt
     3001 tttctgggtc tgctccacta gcaattaata atctagcaat agtagtatta gaagcaccag
     3061 ctccataaag agttattttt acattttcta tttttttatt aactattttc aaagcattca
     3121 aaagtccagc taaagtcacg caggcagtac cctgcgcatc atcatgccat acaggtatat
     3181 cgcagctctc tctcaaagta tctaaaacct tgaaacaatt gggttggcta atatcttcta
     3241 aattaatagc accaaaagag tgctgcacca ttagtacaaa ctttataata ttttcaggat
     3301 cattctgacc tttttcatta cgactatcta tacaaagtgg tactgcgtct ataccaccta
     3361 aatatttcat caggaatgct ttgccctcca ttacacctaa tccacctggt ggagttacat
     3421 caccatcacc taacacacgt gtagaatcac taactacggc aactagattg ccacgattgc
     3481 ttagttcaaa tgattcatca ttattatctc taatcagagt gctaatcgag ctaacgcccg
     3541 gcgtatacca cacattaaac caattaaaac ctggcgtagg tactttggga gctatttgta
     3601 tctttccatt ataatatcta tgtgatagct cagataattt tttaaaaaaa atagttttag
     3661 ctcttgataa ttgctcttcg ttaaaagaaa cggggaataa agaatcaaga ttttcaagag
     3721 aaatattatt tttcataatt atatattatt tatttttgcg gaaaagtaaa tgcatatcct
     3781 aaggttataa cagtgttata tactcctcga ttaaaatagt atgtagcacc agagtaatgc
     3841 ttgcgaatag gtaatataga ttgatctagt tcaaaaaaca tctgatgaga ttcattaaaa
     3901 ttaaagttaa atcccacaag gcagctaaat tcaaatttat aaaatggttc tagcattacg
     3961 actagtccat tctcatccca aatattgtcc ttggtttttg taagaatccc accagcaagt
     4021 ccaacaaaga catataaatg ttttataggt agcttatatt cgaatagtaa tggtatttct
     4081 acataattta actttaaaag aaaacttgta ggatcttcac ttgtaggatt ctttctacta
     4141 cctttttgtg aaaatactaa ttctaatttc aatttagact gttctgaaat attataaaaa
     4201 gtaaaaacac cagttctaac gccaaattta tcaaatccag acaattgatc acctgatact
     4261 tggctaccat tcaatgcgat gctaaatcca ccaccaaaat tttgtgcagt taaaaatgtt
     4321 gaaattaata atagaattag aaaaatgaat tttttcatga ttttataaat tctcattaaa
     4381 ataattttct attaaatcat tgagatgata acgatctaca cccttgacat tatgactatg
     4441 agtagggtag acaaagaaat ctactaattt acctttatct atgcaagctt ttaagaaact
     4501 aagtacatgt tgcggtacta ctacatcatc tatatatcca tgtatcaaaa gtattttgtc
     4561 atgcacatta tctacgtagt ttaaaatact tgtatttttg tatccatcgg gattttgctc
     4621 tggggtatcc atgtatcttt cgccatacat tacttcgtaa tatttccaat ctaccacagc
     4681 gccaccagct acagcaactt tgaatatctc aggatgcttt agcatcaaat ttaaagtcat
     4741 aaatccacca taactccatc catgaacgcc tattctatta gtatccaccc atgatttttg
     4801 ctttaaccat gaaatacctg tcatctgatc ttctacttcg tattttccaa gatttctata
     4861 aataatactt tcaaattcga aacctctatt agcagatcca cgattatcta atgtgaaaac
     4921 tacataacct ttgctagcta aataaggtaa gaaagtacct gctccgcctg tccagctgtc
     4981 agttatcaat tgtgcatgtg gtcctccata tacgtatact attataggat atttttttgt
     5041 actatcaaaa tttagaggta aaaataatcg cgaatacaat gtatcgccat tattatttac
     5101 taaataaaaa atatcaggtt taggcaaaat ttctttctga aaaaaatcta ataacatttt
     5161 aatcgtagta gatttatttt ttttgaaatc aataaattga acatgataag cagtatctat
     5221 attagaatac tcatctatgc aggctgtacc attttgattt actactacat aatgagtacc
     5281 cttaccttta tctatctttt taaaattcaa attattcaga tttaccatgt aggcatgacg
     5341 ttcaataggc gaatcataag tcccataaaa ccatacaatc tctccacctt ttaaaaaacc
     5401 accaaactca ttaactatcc atttaccttg agttatttgc ttaattaatt ttccgctaat
     5461 gtcgtacaaa tacaaatgcc aataaccatc tctctgactg aaataaatga attgatcaga
     5521 attattaggt ctaaagtata atggtgtttg tggttctaca tatcgatcat ttgtctcttc
     5581 gaaaagtgtt tttatcatag ttccattatt aacatcatac atgttcatca tcaaatgatt
     5641 ttgatctcta tttaatatac caataaaaat atatttttca cttggatgcc atgtaacact
     5701 agttagataa tgatctaagg tatcacctgt ctgcatataa atctttgact ttttattgat
     5761 atcgtatata ccaagagtca cataatgact ctgcatacca gccattggat atttgatatt
     5821 tttcaatgta gccacacgag ttgttacgtc tacaagtgga taatctgtaa cttttctttg
     5881 atccatacga taataagcca aatatttacc tgtgggcgac caaaatattc cattgtttat
     5941 accaaattca ttacgatgta ctgattgacc ataaacaata ttagtatcta tatcactagc
     6001 tactacactc tgtataccat tatcagcaat atataaatta tttttaatag tatatgccac
     6061 ttgtaatgtt gtgggttcta atgtaacatt tttagcagat tgaggccaat tattaataaa
     6121 ttctattttt tttgtcttaa aattataaat ataaagggta gtatctattg tcattattaa
     6181 agtatcctta gttttccatt gatattttgg gaatgttttc tgattattcg acaaacctgg
     6241 cggtaataat tggttcaatt gatctaagga taataaatta ctaaatttgt tatttttaaa
     6301 taatactaaa ttatttaagc tgtctaccaa ggtaaatgat tcgccatcag gatgcgcttg
     6361 tacctgcatt agaggtgtga atttttgata atatttgaat atatctctat aattgtttat
     6421 cgtttgtgca ttgataaatt gagttacaaa caaacacaaa attaataata ggacttttga
     6481 acttttcata attcttaatt tattttaata agcaaatata ataataaata ataaaagaga
     6541 acattataat tcttaatttt tttaatcagt taagttataa cttgcatcct ataacagtta
     6601 actttaaata atactattat ttaatttgaa tagtaccaag aaatcagaaa aaaatggtaa
     6661 ttgtaaaagg aatcgaaaag agtctagaag caaatcattg aagggtttag aaacaaatca
     6721 ttgttttact tgaagtagtt tatctatatt tcgtattcac cagccgccaa tactcatccc
     6781 acgcccatct gtccgaggat gcgaggttta aaagccgacc aatgtcagat tctatctcac
     6841 ttgtcaagta tttatgtgtg attacaaacc acatcaaata gctctggagg tatttagtag
     6901 ccactcctct aaatcgtcgc aaccaaacct tgaaatcatt tatgtgctta tcgactacct
     6961 gtacactgta tacaccgcgt ctaatctgcg ttttgtttac aacaactttt ttgcttttgg
     7021 tccccttcac agcttttttg aattccttct tttgcttcac acatattaag ttgctatctg
     7081 tcactctatg cgatagctct tctcttatct gactattggt tacttttttg cgtcctacgt
     7141 gtttgaaaag taaattgcct tccctgtctg tcattactag cacagctact ttgggatgtt
     7201 tctttttctt tgctttttcg tattcttttc ggcttctgta tttgcgacct ttttctgaat
     7261 atttcattag cagctcatca gcttctacaa tgcctgagaa gctgatacca ccctgtaatg
     7321 atttgatcgc tgctagtatc ttgtgtcgcc aattgaaact cgttctcaga cttatgccta
     7381 cttccgatgc gcaagctctt agacttttgc cttctaacat gcattttagg taatcgagca
     7441 ttttatcagc ttttcgcatg cgatatacga aggttctagc tgtttcgcgg tattgtcggc
     7501 cgcaatcttt gcatttgtac acttgtttgc cgtcgcgtac tccagctttt attacatttt
     7561 cgctttcgca tctaggacag atgggggctg tgctttgtat ttttagcttc tgataatcta
     7621 cagccgtgta gttgttttct aataaaaatt gatatagatt atctagaaat cggcgttttt
     7681 gttctttggg tagagcctga taatctaata aattgaaatt atccataatt atttttttgt
     7741 aaaattaaat aaaaattatt tgatggtggt ataaaatacg taaagtaaaa caaaaattat
     7801 tcttagaaga ttcccttccc ccttagattt aaaacacaaa cccctatagt ataattatct
     7861 gattatcacc attttaccaa atcctttagt tatatattga tctaattccg tttgaatatg
     7921 ttcgattgga tcgccatgta agcgagttac aactctataa aaatataccc catttgctaa
     7981 taaattacca tacatatctc taccatccca tgtatatgat gttatattcc ttccgatatg
     8041 tatatgacca agctcttccc ttgtaatttc cttaactatt ttgcccgaaa tagtcattat
     8101 ctgaatggta aaatactcag gaatatgact gccagtaata gtaaaaacga aatgtgtctt
     8161 ggtgctaaaa ggatttggcc aattcgttag atatgttatg gctggttttt gaattatttc
     8221 aaatttgatt ttataatcta ttatgccact cggattacca ctacgatcat gagcttgtat
     8281 ctgtaattca tatattccat ctacttcaaa tttagcatac caatctatat agcacacatt
     8341 gctattagca tctgatgagg gagtgaaaat taattgataa gtatttgatt ttacaaaata
     8401 tatcctttca tactcagaag catttggttt ttttaaaaaa atagtaacag cactagtgtc
     8461 atctaatatt aaaaatttgt tttcatctct tacagtgatt tttatatgtg gctcggcact
     8521 tactatatca ccattaagaa tatggatacc atcaaacgta acatctaata atggatttat
     8581 tttgtctgta tttacataaa aagggatact tgcaatatta ttaatatgag tctgttctat
     8641 ttcaaaataa tcaccagtat ttgggtttat tggattaaat tcaacccata actgattaaa
     8701 tcccttcaaa cctatggttg aaaattttat actatcagtc aatacatcat tggcaggatg
     8761 aagtctagtt tttctaagtg ttatatttat ctcttcattt atattagttt taatccagta
     8821 ttttacccat aaagaatctt gaaaatcttg agaactaatg tttttagttg aaatttttag
     8881 tttgcctatt tcgccttctt gcaaagtatc tttgtaaaat tcatattcct tagtaaaact
     8941 taaaatagct gtttcgggaa ccggtgtgta taatacctgc caacgtatag gttgtggagc
     9001 agtatgcata gcatcatcac tagatttcat tagtaatttc aaatacgggt aggtattggc
     9061 tggtatttgt tgatcgaggt tataaatatc taatgaatca gatataggtc tgatattagc
     9121 aaataaagta tctatggttc cattttcttt tataccaaga acaaaaagtc gaactgtatc
     9181 agtgatcggt ccatcttcta agctacgtac acgccaatga atactattcc attgtagagc
     9241 aggtcctatt aatggtgaag acattgatcc ttctttccat tttgtagtta ggttccaaga
     9301 atgattaata atactagata tctcagtacc aattatttcg tctgcagttc ctatagggta
     9361 acctttcctt ccaaaaatca tatacggtct attattaggt aaagagcgga tcattgcaga
     9421 gccaaaactt tcaaaggctt gatagaggtc ttctgggtaa ttttcagcat tatgatttct
     9481 atgactaaaa gctaaaatat aattaccatc tggcactgca tttataaagt ttgtcatacg
     9541 ttgccaccat atactatcgg tagtatagaa atcaaaatca taataaatgt aatttctgca
     9601 attaagagca tcataaggac cgagtccatc attgtctggg tcggtattag cccagggagt
     9661 aatagatata gtatcaaaaa ctgcaaattt cataccatgt cctccatcac ctgtgcaaga
     9721 ccattgccct tttaaaacat tattcatttt ataccattcc tcatgccaag gaatatatgg
     9781 ataatagccg gtttgagctt ttatattaac tattgtattt atataattaa atgttcttgt
     9841 agggcgatga gtagtcatat aaacgaaatc attattttcg aattgaaaga aatgtgcttg
     9901 tccccaacct cttttatttt ttataaattg aaatgaacta ttacgccaat atgatgaggt
     9961 tagagttttt actctccaat aataaactat gctatcaggc atttggagca tcgtaaatgg
    10021 caatgtccat tgtataatac cgccagtttg ttcaatgata ggactttgta gtttaaaagg
    10081 actattgaaa taaatagtag tgtctatctc aaaaatatat ttattcatta atggaaatgg
    10141 attactcgaa gaggctatta gcgttacaaa agtgtctgga attacagcaa attcataagg
    10201 atatattggt actgcatcat aagaattaac ccaaagaata tattcagtag tattattatt
    10261 ttcatttttt tctaatatat cattatacga atctattatt atcttaaatt tattcatgcc
    10321 tatgctaata agagggttgg tgggtattgt aaatataatt gtatctttaa aaaaaggtgt
    10381 tctgattcgt tttatataag tttccgttgt attatctgga taagtgcgaa ttatttctac
    10441 aaaaaccgag tcataaatcg ctttgcccaa gttatgtgca gttacacaaa atttgaaaga
    10501 atctatttcg ttactgataa tttctggttg agtgtaaata gtctgattag taaccatata
    10561 atctggcaaa ctatcgatag aaagtctgat aactggatca caatgcaaga taaaatcgta
    10621 acaaataact ttattataaa aattatttgg tacttgtata tctcttatag tatttttaac
    10681 tatttctcct atacttttgc cataggattc tattgataaa tgattaatca actgattact
    10741 aaataaatgt aaataagaag tatacgacgc agtattagta cccaaaaaac ctatcatacc
    10801 tttattaggt attaaaacaa attgttcgct ggtagtggca tctacttgaa ataaatcacc
    10861 tacaaaacaa gaattagcta aaacaaaagg ataccgtttg taattattat aagtatttgg
    10921 attatctata ctaatatcaa agcctaatcc tgcagcatga ccgaaaaaat tcatcattat
    10981 cacgcctcta tctactaaat ttctaatact atcactaaga ctttgtatta tgggatcagt
    11041 actagtttta gtaaatgtcc atacattacc tccgaaataa gggttttcaa ttttattttt
    11101 atattgatta agataagata aaaattgatg catttctact tgattggagc ctcctgcaaa
    11161 atgtaatacc ttttttctcc attcttgatc tatgggtaag ttaggattat agggtagttt
    11221 catttcattt tcgaaatcaa taactttttg caaataaaca tccacatcat taggattgcg
    11281 agctgctaag cgacctattg gtattgctgg ttgatacaaa gtatctacta taccagataa
    11341 aaataattga tcagatggtg gattaccaat tgatggaact aaggtttgag catagatatc
    11401 aggctttttt ctataattgt agttaccttc ccctgcttta tatggtttac ctattaaaaa
    11461 tactcctttt atcggaacct cagcttttgt aatggcaaac aataaaaagt tcctgattcc
    11521 cagtggatgt ttgccaatgc cataggcaaa ttgatcatat agctcttcta catttattac
    11581 taatggtgaa tatcctgtta tagacctata atttgcatag ttatttgcgc tattgattaa
    11641 aattggatgg gtaatgataa gataatctgc cttaataagg cctaatgata aataatcagt
    11701 gaatttacct gttccgttta tagcagttaa ctgagtaatt ttttttacat tactttctat
    11761 agtgctataa atttctacgt cattaccatt cattgctatg caaatgtcta cattgttatt
    11821 atttttagta gaaaagattc tattgttatt tgtcaaatca taactaataa gagtatcatt
    11881 attattgcca tagacattaa taaaatgtat gtatctgaca ttttgtccat tatcttttat
    11941 tcgatatttg agagaataag aattattata atttgttaat ctaggataag aaacttcaat
    12001 atatgatagt gctatacgat cagaaggagc acctagatca ttgggcaagg taaaattaat
    12061 atttagatta gatgatagag agctagataa aaaatttcgt gagataaaaa cattcttata
    12121 tcctataaaa gtcgtgtcaa tattattaga taaaaaatta atttttagat gatgtggata
    12181 atcgagtgct gatgattgtc cagtgatcat tattttgatc tctgatggac ctcccgcatt
    12241 atatgggaga ggtgtttgta tttgcttatt ataattttga ggaacacccg gctgagaagg
    12301 atttatgctc atagtaccat caaaccatcc ttctgcttcg gtataatcaa aatctgataa
    12361 cccagaagcg tctactggtc caggataata atagcttgtg taatctattc gatttgtata
    12421 gtagacatgc gattcaggaa aataagaaag atactctgta tttttataat ttgctactct
    12481 cttattattt aaagatgaac tccatgtgag aaaatatgca gacgtatcat tgaataagct
    12541 gtaattatca ttagcttgat gttctgaatt tttatatagt tttttatcga agctaccatc
    12601 attaccttta gcataaaatt ctataaaatc gtcattatca aatataccat cctcttcgcc
    12661 atgtacatag atatattgtt caataccatt atgaaaaatt tgaaataagc gagggtctat
    12721 tatatttact ggaatacctg cagatactaa ggtattataa tctatccgat atatgccttc
    12781 ggaactgatt tttattttgt aataaaattt attgtaattt atccactcat taccaaaagg
    12841 ttgcgcaaat aatttattag taattataaa aattattaag taaaaatatt taaactgttt
    12901 cataaagctg tgttactatt ttttttgatg tgataaatta acgatatgat attagaatac
    12961 agagcaatac tattatcacc gatatctgta taagcatagt ctatttgcaa gatatctttt
    13021 atagcgatgc ctatacctat attaggctgg aatgtccatt ttttgccagt agggaaattt
    13081 tctttttgta agctgccaat gccacctctg agaaatatca tttgtttaaa gcttatctca
    13141 aaacctagtg atggatctat gctaaaaaat ttattgctta tcaaagtatt acgttgtccg
    13201 tcaaaagtaa atagtaggga aagctcgggt tgaattaaaa aattatttaa tagaggttgc
    13261 acataacaga tacccatggc taaacgagga atacctaatt ctaaactatt agtaggtatt
    13321 tcattaccag tgagagtaaa aacttctttc attttatcat ttaatgaaaa agaccaaaaa
    13381 gtaggggtag aagtaatatc tcgcactact aaacctcccc tccatctatc actcataaaa
    13441 gaaacaccca catctagtcc aaagccccat gcaccagcga aatcacctat tactcttcta
    13501 atgattttta aagtacctcc tatctgcaat gtgtgattaa tctttttgct aatactaaat
    13561 agaagggcat tatcagcagc tgtgaatgaa gtgattttat cataatgcac ctgcccattg
    13621 ttgtctatta gttgtgtcgt atttggtatg tcgtctactg caaaacgtaa ataggaaaat
    13681 gcaaatgcta aactatcttt attatgaaaa ctaatagcaa atgaattata attagctata
    13741 ccagcaaaat attctgcgtg cattagacta aattgaggat atgcaggagc gaaaactagt
    13801 agagcaggat tccaataacc agcattcgca ttgtatgtgc tagcaatagc agtattactc
    13861 atagccatag atctagcatc tatacccaga tagagaaatt cattagtata gcttctcact
    13921 tgagaatata aaattgtaga aataataagg ctaattataa atgatatata ttgcctcatt
    13981 tattaaattt atttgtaaaa ttaaataata aatattaatt ttataatttt aacttacata
    14041 taatttattt tctattataa aatcataaac agattttctt agaaaaaaac gaagatcttt
    14101 attttgtgat aattgctctc taatccattt actacaaata tctatatatg gagcattaga
    14161 atctataaag atatttttat gtttaaaaat atttgatgat aaaaagccat ttctagggta
    14221 aacatatata gtgaaattat caataagata ttgataattt tcccatttat ctaatgatac
    14281 agcattatca ctgcctatta ttatagaaaa attgtaatga ggaaatctcg tagtaagtgt
    14341 ttctaaggtt ttataagtat agctgggttt cggtaaatca aattctacat cactagcttt
    14401 aagaaaatga gcatcttcta tagccaactc taccattttt aatcttaaat ttgcatctaa
    14461 taaatttgaa tttttcttaa aaggattttg gggggaaaca ataagccata tttcatcaaa
    14521 atcatttgta atgtaaaaat agtttgctat taaggtatgt ccgatatgtg gtggattaaa
    14581 tgtcccaaag aataaaccta tatttttttg atcaatcata aatattttgt taaataaaat
    14641 tccaaatttt aacaaatata tataatttta aaataactaa ctaaaaaaag taaattattt
    14701 atattttttt ttaaaataga gccataaaaa aattcattaa caaacttatc aaaaattctt
    14761 atttttagta tctattatta tcgtaacagg cccatcattt ataagttcta cctgcatata
    14821 agccccgaat acaccacagg ctataggttt atgaaagaca ttttgaagga tttcaataaa
    14881 ttgtttataa taattttcac tcacctgaaa aggtgctgca cgagcaaatg agggtctatt
    14941 tccttttttt gtccaagcat gcaaagtaaa ttgactgaca actaatatat ctccatcaat
    15001 gtctgatact gaaagattca ttaatccttt agtatcgttg aagatacgta aattaataat
    15061 tttatttgtt aaccatttaa aatcttcttt agtctccagc tcctcgaatc ctattaatat
    15121 taataatcct tgactgatag aggctataat ttgattaccc acttttacag aagccttaga
    15181 tactcgctgt ataactgcac gcattatttt tcattataaa tcaaataagt taaaacgtta
    15241 cctacaactt ttaaagtatt tttatctatt ttgtctatat catcttccat agtatgccag
    15301 taagggaaaa aaccagttgg gcttgtttta tcctgatgaa caatattaat ggtaggtatc
    15361 tgtgctattg tattcaaata caaatgatca tcagtgatag ggttagaata atctggtata
    15421 aaatatttac tataacctaa tttagaagca atatcccaag ttttttttaa tattgatgag
    15481 gcatattgat aagaaatact ttcaaaataa aaagtagcat cagtagcacc taccatatct
    15541 aataaaatac cataattagc attataaaac atcggcactt tatttttaca ccaatattga
    15601 ctacctaaac accaatattc acctccataa ttattcacat tttgtggttc gccccaatct
    15661 tctaaatccc aaaaagctat atctatgcca atcgaaatat tttgtttatg gatgatacgt
    15721 gccaattcta ataaaacacc tacaccagaa gcaccatcat tagctccagg tatgggcaaa
    15781 ttatgatttt taggatctgg atcataatca gcataaggtc tactatccca atgagcacat
    15841 aataagatgc gcttttgaat atctggatta aactgagcaa aaatattgcg accttctttt
    15901 acttctccag tgtatatacg agctttgaat ggttgtacat agacagtatc tgcatatttg
    15961 tcgagcatag ataccagcca gtcaccacac aatttatgag attgtgtccc aggaactctg
    16021 gggccgaaat ctacctgctt agctacataa gtataagcgc tatcacaatt aaaatcagga
    16081 actatgattt ctgttttttc ttctatttct ccagttactt tagcttttct tttacaactt
    16141 tcagaaagta tcaaacaaaa tatcaataat aaacacaaca atatgtggat caatcttcta
    16201 tttcccatgt atatttatct tttaaatcat aaattttaat tcctaactca atcaatctat
    16261 ttctaatatc atctgcaagt gcataatttt tttgttgttt tagttgccac ctaatatcta
    16321 atattagatc tataaatggt gcaatatctt taacattttg agaactttct aatttaatac
    16381 ctaaaatatt ttctattata gtatttttca tccaatatag tatctcaata tcatcagaag
    16441 atagattaat cttttgctca ctagccaaat taatgtgttt aacagcttcg aatagattag
    16501 caatagctat aggagtattt aaatcatcat tgagagctga taaaatatta tttttaagat
    16561 ttaagagtgg caaatcagtt ttatcagtat taggagataa tttttcgatt ttcataaaag
    16621 cttttgttaa tctattgagt ccgccttcag ctgccttgag tgcttcatta gaaaaatcta
    16681 atgggctacg ataatgtgct tgcaaaataa aaaatctaat taccataggc gaaaaagcat
    16741 tttcgagagc tggatgattg ccagcaaaaa gttcttcgag agttatgaaa ttattaagac
    16801 ttttgctcat tttttgtcca ttaatggtaa tcaaattatt atgtacccag tattttactg
    16861 gttgtacacc tttagcagcg acagcctgtg caatttcaca ctcatgatgt ggaaattgta
    16921 agtctatacc tccaccatga atatcaaaag gaatacctaa atatttagca cccatcacag
    16981 agcattctaa atgccaacct ggaaatcctt tactccaagg agaattccat tgcattatat
    17041 gctgaggtgt ggcttttttc catagtgcaa agtcataagg gctctttttc tcttctttgc
    17101 cctgaagttc tcttgtatgt gctaataaat cttctaattt acgtccactc aaaataccat
    17161 ataaattagt atcattttga tatttaataa catcgaaata tacagagcca ttagatagat
    17221 atgcatatcc attatctaaa atttcttgaa ttaattctat ctgctctggt atatgtcctg
    17281 aagcaaatgg ctcaatagat ggaggcatga tatttaataa atctaaggct tgatgatatc
    17341 tacgagtata ataatttact atttccatgg gttctaactg ttctaatctc gcttttttct
    17401 ggattttatc ttccccatca tcagcatcat tttctaaatg acctacgtct gttatatttc
    17461 gtacatatct aactttgtaa ccaagttctt tcaaatatct aaacaaaaca tcaaaaatga
    17521 tggctggacg tgcatgacct agatgtggat caccatatac tgtgggacca catacataaa
    17581 gccctacaaa tggctcatta ataggcacaa aacgatctaa tttcctagtc aaactattat
    17641 acaaatataa tggatgatcc atgaaacaaa tttttttcga aattataaag aataattgaa
    17701 ataagtattt aactaatact actacaatta gtaatataat tgtaacaaaa attatgcaat
    17761 ttttgattta tcaaagaaat acaagggtta agtaaacatt attaatcatt aaaattgaat
    17821 ttcaattttg tattttgcac caacaaaact agataggcag aagtatataa gtagtttagt
    17881 atcttttttt gattagcttc gaaatttttg aaatgttcaa agcaattgta agacccgttt
    17941 tttagtcaat gtctggatat atttatcata actattctaa aaattaaaaa tcaagtagtt
    18001 gtgaaaataa ataattttgt aaattaaata tgagagtcat aaaatattta agaaaaaact
    18061 tgtgatatat ttgtaatgag gataatgact aatagagtat ataaaaaata taggtactaa
    18121 tgaaaatgca gtaaactcac aaacatacaa agcattaata taaatatctc aatgctatat
    18181 tataataata aaaacactta attaattttt ttttattttt gcaataattt caatttaaaa
    18241 tatttaagat ttttgtcaaa tgatatattt taatatcaaa aatacaaatg aaaaaaaata
    18301 aatcatacta ccaaagctta cattgggaaa atttatattt aaaaccatat aaaactacta
    18361 aacgttatat atctattgta ttaggagtca tagctacagc tttagcatta ggttttatat
    18421 ttacattaat agctatttac atttttccat caaaagagga ggagaaaaaa gcttatgaat
    18481 tagcacaatt agaaaaaata tatgaagaaa tacaaaataa tcaaaaacaa atagaaatat
    18541 tattagatac tctatctata aaaaataata ttatattcaa aaacattttt aatacagatt
    18601 atattgacga agataaagag atgaatactt caaaaataac aagtgcaaaa tctttatctg
    18661 atatgttaaa aattattgat gaaaatttga ctaaaataaa gcaaaaatca aatattcaat
    18721 attattatta taattttcta tttcattatt taccagaaaa tattaatgat ttaatgaaat
    18781 taccaattcg catgccatta aaagaaaact acgagatagc aagtggattt ggagaacgaa
    18841 tacatccaat tctcaaaaat actttttttc atactggcat tgattttgcg gctccattac
    18901 gtactcctgt gtatgcaact gctagtggag tagttagaaa tccagataaa tcaatgtttg
    18961 gctatggtga aactattatt atagatcatg gcaacaatat atctactgtt tatgcacatt
    19021 tattagaaaa gaaagtagca actggacgca aagtatctgc aggtgagttg ataggttatg
    19081 taggtaatac tgggatttct tttggaatac atctacatta tgagattagg atcaaaaata
    19141 atcccgttaa ccctattcaa ttttttataa ctttagatcc ccaaatattt tatcattatt
    19201 atttacaagc taaaattttc aatcaagcac tttcgtgatt ttttattttt atcaatgatt
    19261 atatccatta tttttatctc acgtttgcta acatataaaa aaaattaaat ttccacatac
    19321 aattttacct tttctacttc tcacccactc tcttactaag attatctatt attgtaattt
    19381 actaacataa taaatataat tctaaatttg tacttttatc cttatttaaa ctttctaagc
    19441 ttattttgat atattaaatt tttgttattt ttggaaatga aaatgaggta atgattaaga
    19501 aaaagaagcg acccacattc gattttctaa aaattggcta tagagctgtt atcaatcaag
    19561 ctattattcc agctattatt attggtataa taactgggct agcagcagta ctattaaaaa
    19621 atggtgtata taaccttacg gcttgggtac aagatattac acgtgtagga catttttcgt
    19681 tactttattt ttttctaccc attattggta ttatattaac tgtgattttt acagataata
    19741 taattaaaga aaaattagac cacggggtta cacgggtttt gcaatctata acaaaaaata
    19801 agggagatat tcatccacat aatatttatt caaatatagt agcttgtgtt atcactgctg
    19861 cttttggtgg ttctatcggt atggagggac ctatagtaac tactggtgct gctataggtt
    19921 caaatacggc taaatttttt aattattcat ataaatataa acttctattt atcagtgcag
    19981 gaatgtctgc agctatagct gctattttca aagctccaat tgctggttta attttttcta
    20041 ttgaagtatt agctatggat atcactgctg gatttttgat tcctgtagtg ctcagttgtg
    20101 ttacagctac tatagtagct acattttttt taggtcaaaa agcagaattt tattttgcag
    20161 tttttactaa ttataattat tctaatactt tattcttttt tttacttggc atatttgctg
    20221 gtttactatc tatttatttc ttacgtactt tcagttttgt agaaaaaaaa ttatctaaaa
    20281 taaaaaagaa atctttcaaa atattgatag gtgggggatt attaggattg ttaatatatt
    20341 tatttccacc tttatatggt gaaggataca atagcatgga gagtatttta agtgggaaaa
    20401 ttgaacaact attttcttct tctatcattt atccatattt taattctcaa gtaggatttt
    20461 taatattatt aataatatta ttttttatga aagtaatagc tacagctctc actactggtg
    20521 ctggaggtgt aggtggtgta tttgctccag ctctatttac aggtgctgta ggtggtactg
    20581 cttttgcttt gatgatgaat cttatattta atacaagttt aaatattaat aattttacac
    20641 ttgtaggtat ggctggtgta atggctggcg taatacagac accactagct gcaatatttt
    20701 tgatagcaga gcttacaggt ggttataatc tttttatccc gcttattata actgctacta
    20761 ttgcctatgg cactactaga ctagtggaac cttattctat ttatactaaa cctctagctg
    20821 aacgtggaga attacttact catgacaaag aaaagaatgt ttttacttta ttagataaag
    20881 atgaactgat agaaactgaa ttttatcctc ttactgtaaa tcaaaaatta gccgacttag
    20941 tcgatgctat caaaaattgc gatagaaatg ttttccctgt tatcgataac tctagtaatc
    21001 tagttgggct gattcatttt aaagatatta aaaaaattat atttgaacca gataaatatg
    21061 ataaagttta tatcaaagat ataatgaaaa aaccagaaat agtaatcgat attaatgaat
    21121 ctgtggattc tataattgaa aaagtcagag ccaaaaatat ttggaatata cctgttgtag
    21181 ataatggaaa atataaaggt tttatttcta aatcttcttt attatctgcc tatcaagaag
    21241 ttttcgatga atttattttt gaataatttt ttttcaaatt aaactttgaa atattttttt
    21301 taacatcatt tattttagtt tatatttttt tttgaataaa aatctatctt ggtatttcat
    21361 tatctatttg taattttgtg gtgacatgat gacgatatat ttacataatc cactgactat
    21421 aagcctttta atagtttttt ttcttttatt actttattat ttagttatct ttatccgatt
    21481 agcattttac aaaaaaaccg ttaataaaaa atatcctcct gttagtattg ttattgttgc
    21541 taaagaagaa ggatttaatc taaaaaatca tttacccctc atatgtgagc aaagatatcc
    21601 taatcaattc gaagtgctag tggtggatga taattcgact gataacagcg aagatattat
    21661 ttgtgaacta aaatataaat actctaatct aaattatctg agattagata ataaaatgtt
    21721 tgaaatttat aaaggaaaaa aattcccatt atcaatagga ataaaaaata ctaaattcga
    21781 ttatttatta ttgacagatg ctgattgtaa acctgcatca gatctttggt tgatgactat
    21841 gatggaggca tattcagaca agactgagat agtcttaggt tatggcaaat atgagaagac
    21901 aaaaggattt ctcaatacac ttattcgttg ggatactttt tttcattctg ttatgtactt
    21961 ttctgccgct atatgggggt ttccttatat gggcgtcggg cgtaacttgt cttataaaaa
    22021 ggaacttttt tttagggtaa aaggatttac taatcatatg aatctaatga caggcgatga
    22081 tgatttattt ataaaagatg ccgctaataa agataatgta tctatttgta tagatcacaa
    22141 agcccatact atatcaaagg ctcctaaaag cttgaacaaa tattttattc agaaaaaaag
    22201 acattttaag agttcatctt attacaaaat tgttcctcag atatatttaa ctttgataca
    22261 ttgcctacag atagcatttc ctgtgttgat aattttacaa ataataattc aaaaatttta
    22321 tatctttgct attagtatat ttataataaa agctattatt caagtgttag ttttaaataa
    22381 aataaataaa aacttaaaag aaaaaaatct aatatggatg gcgccaattt tagatttttt
    22441 aaattcaata tttcttttta gtttctttat tatcagtatt tttaataaag aaactaaatg
    22501 gaaatagtat ttttctaaaa aaaatatgat gctaaaagat aaaaaaaaga taaatgaact
    22561 tgttattaac gcaaagaatg gtgatagtat ggcttttgcc acattgatga ataattataa
    22621 ggatggatta tattttttta ttataaaaat ggtaggtgac agggtagcag cagaagatat
    22681 attaatggaa accttttata aagcatttac aaatttgtct ctttattcct tcgattacgc
    22741 ttttagtact tggttataca ctattgcaaa aaataataca attgattaca ttcgcaattg
    22801 taaaaaaaat gttttacaca attgcaaaaa agattttatc aatgatgatg caagtagttt
    22861 tttaaatgac atagctgagg ataaaaaaca tgaacccgat aatattgtta ttcgacaaga
    22921 agaaaaagaa gctgtgagag attacgtttc tatgttaaaa cctcattata ggattttagt
    22981 agaaaaattt tatttcgaag ataaaaaata tgaagagata gccgaagaac tcaatttacc
    23041 tctaggtacc gtaaaaggga aacttcatag agccagaaaa atgattaata aatttatagg
    23101 acaaaataat atataattat gtctaatggc gttatatttt agcttaaact atttataatt
    23161 cagacatgta ttagttgaaa tgatttttta gataactttt aatattatat ttgtaaataa
    23221 tttgtaaata atgtaaaaaa tatcaacatg gaagaaaaga aattgatgcc aaatttttta
    23281 ttcgaagtta gttgggaagt ttgtaataaa gtgggtggca tatttacagt cttatccact
    23341 aaggcttata aattagtgga tttgctaggt tcacaatata tacttatagg tcctgatata
    23401 gtaaaagatg cagctaatgg ttatatgttt gaacctgatg aaggtttgta tcataaatgg
    23461 gtattgaaag cacgagaaga tggattacga atcagaatcg gaagatggaa aataaagggc
    23521 agtcccataa ctattctagt agactttagg catttatttg aacaaagaaa taagattttt
    23581 acagatctat ggctaaaata caagttagac tctttatatg gaggctggga ttatatagaa
    23641 ccagctttgt ttggttatga ggcaggtaga gtgattcatc acttttatga atatcatatt
    23701 acagctcagg ataaaatagt tgccaatttt catgaatggc ttactggtgc tggtatttta
    23761 tatctagagg ataaagtacc tcaagtgggc accgctttta caacacacgc taccattatg
    23821 ggaaggacta ttgctggtaa tggactccca ttatatagtg aaatgactaa ttatgatcca
    23881 cagcatatgg ctcaaaagtt taatataatt tctaaatttt caatggaata ctgtgctgcc
    23941 agatgtgctg atgcattttc tactgtatct cctgtgactg ctaaagaatg taaatacttt
    24001 ttagataaag aacctaacgt tataacacct aatagctttg atatagatct agtaccccaa
    24061 ggggatgaat atgaaaaaat taaagccgct tctcgcgaaa aaattattag attatttaaa
    24121 gctactagcg gtgcagaaga tcctaatccc tttttaatat tgttatcagg tagatacgaa
    24181 atgcgtaaca aaggcataga cctatttata aaatctttag gaaaaataaa aaatcaaaat
    24241 cctaacagaa ctatctatgc ttgtatcgct gtgcctgctg gcatacaagg acctattcat
    24301 gatgtgctag atgcttacaa taataactca gtagccatta aaaaatatct aacatcacac
    24361 tacctaagta atgaagatca tgatcctata acaaacgctt tcaaagctga aggactaata
    24421 aatcaagatg ataatccagt taaagtttta tttattcctt cgtatttaga tgggcatgat
    24481 ggacttttga atattcctta ttatgaattt ttgatgggct ttgaccttac acttttccca
    24541 tcttattatg aaccttgggg ttacactcct atggagagta ccgcttatgg cattcctact
    24601 ctctctacga gtctctctgg atatggtaac tgggttaaat ctctaaatat tgatacttct
    24661 caatatataa ggataatcga aaggaatgat tataatgatg atgatgtagt aaaaaatata
    24721 gttagctatg tctttgaaca attacaactt agcgaagagc aaagaaaatc attaagagat
    24781 aaatgttggc aagtagctaa gttagctcat tggaataatt ttatttgcaa ttattttgat
    24841 ctttacgatt ctgctattag agaatcagaa aagagattag atctttatta tttcaaaaca
    24901 ataaaggatt atgtagttac tagtccaaaa gaaattgaga tagcagaatg gcgcaaagta
    24961 tatgtaaaac ctgaatatcc agcttcgctt ttacctctag tagatatgat acaaaatctt
    25021 tggtggacat gggatgaaga agccatagaa ttattaaaaa atattaatcc tgtatattgg
    25081 attaaatctg agaataatcc aattgctatg ttagagatga tgtcttatga agagataata
    25141 aatttatcaa aagatcaaga ttttatagaa aaattaaatt ccatatacaa aaggttcact
    25201 gactatatga gcatatctcc atataaagaa aatgataaat tagtagcata tctatgtatg
    25261 gaatacggta ttcatgcttt tttgaaaatt tatagtggtg gtttgggtat tttagcaggt
    25321 gattatctaa aggaagctag tgatagtaat ttccctatag tagccttcgg tctattattt
    25381 agatacggct atttcaaaca aaaactatct cgcctaggag agcaaatcgt acaatatata
    25441 ccacaaaaat ttactaattt acctattacc gctgtaagaa ataatgatgg ccagtggcta
    25501 aaaatacatt tacctttgcc agggagaaat gtttatgcta aaatctggca agtaaaagtg
    25561 gggagaatag ctctatattt attggatact gatatagaag aaaacctaga tgaagataaa
    25621 gaaataactg ctagactata cgatgccgag tgggaaatga gattaaaaca agaatatctt
    25681 cttggtttcg gttctataga tgctatgaga gcaatgggga taaagcctac ggtatttcat
    25741 ttgaatgagg ggcacgctgc ttttgctaat attgctagat tgagatatta tattaaagaa
    25801 aaacattttt ctatgcaaca tgcattagag ttggttaaaa aaacttctat atttactact
    25861 catactccaa taccagcggg acatgataaa ttttccgaag atctcatgcg tacttatttt
    25921 gctcatattc cagaatcatt agatattaca tgggaagaat ttatggattt tggcagagaa
    25981 catcgtttag aaactaaatt ttcagttact catttagcta ttaaaacctc tacatacgtc
    26041 aatgctgtta gcaaaattca taagcgagtt acctgttcta tgttcaaaga tttatataaa
    26101 ggatttttcg aaagtgagtt attttttgat tatgttacaa atgcagtgca tcctaaaact
    26161 tggatgacta gtgactggca aaaattattt ctagattgtg ctggtagcga ctttttcgaa
    26221 catatgcacg agaaccataa ttactggaaa tttatagata atctgaaacc ggctactata
    26281 tggaatctaa agctaaaaca aaaaaatgaa ctttatgatt ctataataga aagattgggc
    26341 atcgagatgc ctcaaagaca agaatcacct acacaaatca ttcgtacttt agaagaatta
    26401 aataaaactc ctgaaattct tacttttggc tttgctcgca ggtttgctac ttataaaaga
    26461 gctcatcttt tatttattaa tttaaaacgt ttggctgata ttgttaataa cccacattat
    26521 cctgttcgct ttatttttag tggcaaagct catccttctg ataaagctgg cgaagatctg
    26581 atcaagcgaa tcatagaagt atcgcgtatg cctgaattta tcggtaaaat tatttttatt
    26641 gaaaattatg atactgaatt agctaaatta cttgtaaaag gtgtagatgt atggttaaat
    26701 actcctacta gacctttgga agctagtggt acctctggta tgaaagctgt tatgaatggt
    26761 actttaaatt ttagtgtatt agatggatgg tgggcagaag gttacgtacc tggagctgga
    26821 tgggcactga ggcaagaaaa cacttatgat gacacaaaac ttcaagatga attagatgca
    26881 gaaatcattt atagcatcat cgaagacgag atagcaccta cattttatga tagagataat
    26941 ataggtatac cacaaaaatg ggtagaaatg atgaaaaatg cttattttta cattgctcct
    27001 cattttatca ctaaacgcat gcttttagaa tatgatgata aattttatga taatttaaat
    27061 aactattact acacactgac taatgatgat cataaagttt tgtttgaatt tatttcatgg
    27121 aaaagaaaag tgctaagaaa ctgggatgaa atacatatca tagatatgga aatgtatgac
    27181 agcagcaaag ggcccctacc acttggagaa aaatttactg ctaaattagt catggatctc
    27241 aaagaactta aacctgatga tgtagttatc gaacttattt ttggcaaaaa agtagaagga
    27301 cgcattgtag atattgaatt tactagaact tttgatttta ttggttgtga tgacactaaa
    27361 actatttatg aatgctcgat gcctatggag caaagtggcg tttataattt ttctatcaga
    27421 atgagaccca atcatccttt attagctcat aaacaagact tttatttagt tcgttggttg
    27481 taaattttat atcgctttat tgaactaggt atttttgcaa tataattatt gaaaaaatca
    27541 tagacaatat cattattatg tcatattcta agcaattgtg atattgtttt tgctcattat
    27601 tttttttaat ttatttttta tagagtaaat aattataagt attgataagt tatttttagt
    27661 atttagttga aagttttttt tctttttacc catttttaca tttattatta tttttactaa
    27721 ttatttacta aatcaaatat catcttgtaa gtgtataaat attgtgtttt tattatttta
    27781 tttcttattc acattaatat tttctctcaa aacataatag atacgacatt tatcattcat
    27841 tcataccaag acactataga tattaataac tccgaaatat tatatataaa attgttaata
    27901 gatacaaata aattgattaa taatcaaata ttggacatcg aaaatcaaaa aatctatata
    27961 aatattgaca cgtctattca tttacctttt acatatagat gccgattaag atatattaaa
    28021 aaaaaaatat ctactactct tttatctatt atctacaaag atacattgca aagcgaggag
    28081 ctatcacctt ttcatgttat tgtacctgaa attcatccag aagaaagagg agaaatatat
    28141 acaaatggtt atatatctcg tagtataatg atgagcagta gaggacagag ctcagtacag
    28201 tctgcattag atttgcaatt ttatggtgag atagcccctt ttcaaataga aggcaatcta
    28261 aatgataaaa atttacctgt acaacccgaa ggcactagtg ccagattgca agaaatagat
    28321 agagtataca taacattaaa aaaagataat ttcaagttta cactcggcga tttcaatatt
    28381 catagtcccg aaaatgcagt gctatctaaa tattcacgta agcttcaagg aactagtttt
    28441 tctaatatta gtaaaactga aaaacattct ttcaattatg gaggggtggt agctataaac
    28501 aaagggaaat tttctagaat agagttgaaa cccatagacg gtgtctctgg accatacaaa
    28561 ctacaaacta tagaggctaa ctatatacaa gtaatagcag gatctgaaaa agtttattta
    28621 gatggcattt tactcaaacg tggctatgac gcagactatg taatagatta taataccgca
    28681 gaaatcactt ttatgcctaa aattttcata cgcacatcca tgcatatcgt aatagagttt
    28741 ttatataccg actataatta tgctcaagct tcttatggtt tatatacaga aatctctgga
    28801 gacaaatggc agtggggaat caattatttt tctgagggag atataaaagc tcaacccttg
    28861 ttactaagtt tatctgatag tgacaaggtg atactagctg aagctggcgc caatgtacct
    28921 gttaaaattc catcatttag agctactaat tacaaagatc aaaatattac gtatgctatt
    28981 atagatacgc tagactatga ctcagtgttt gtttactcac gagatagtac tacacaactc
    29041 tataaagttg atttcacata tgtaggcaaa aacaaaggaa attatatagt gagcaattat
    29101 gctataaatg gtaaaatata tgaatggata gcgccaataa atggaatacc tcaaggtgaa
    29161 tatgagccaa ttcgcatact aaatgctccc aaacgtatca atttaataga atcttatttt
    29221 ttttataaac cttcagaaaa cttagaaacg cggataacaa tcggatactc acaaaatgat
    29281 ataaatacat ttagtgaaca agataataaa agtcaaggat tagcatataa atttgaatta
    29341 ataggtaaac catcatctga gcatactcta actttttttt ctcagggtgt gcaatcacaa
    29401 tttacctcat tagaacctct atacaatccc gaagaaaata aaattttcaa tagggaatta
    29461 tatacctctg aaaagatgga attatggaat ggattaattt ataattttaa aaacgaaaaa
    29521 aataattttg cacatataaa aacttcgttt tttaataaat caaaaaatga atattctatc
    29581 atagcaaatg caaatgcaag atattattgg caaaaatttc atttcgtaac tactaatgat
    29641 tattcgcata cacatttgcc atcatatcag attgattatc taaagcatag ttctaacatt
    29701 ggctttaaca ttaaaactct gtggctaggt atagataata aaatagaata tcatcctatt
    29761 tacaaaaaca aaataatgct aaataattca caggaatatt attctttctc accttatata
    29821 ggctggggag atagtacaca aacatttttg aaattatact acgaatatta caatgaaaaa
    29881 atgccattga ttcgtaagca acataaaact ggctatcaat tagaaataaa aaaaataata
    29941 tggagtctaa gacaaaataa ttatttcgtt atgcaaacaa gtgatacagt taaaaaaaat
    30001 tattttacca gtgataatga ggttaattta tacactaaaa ataaatcttt taatatgcaa
    30061 ctacgatata ttttgtctca gagacagtca ccacgaatag attatactta tatcgaagta
    30121 ccgatagggc aaggctcaca ctactggata gatgctaata ataatggatt gcaagagtta
    30181 gatgaatttc acatagctaa ctttaatgat caagctcaat atatattgct tactatccct
    30241 acacaggatt acatcagctc gtatcatcaa acatgtagtt tcaattttag attttcacct
    30301 caactaataa aaaaattccc ggtattttta aagaatttta gcttgcaatc attttttgat
    30361 ataaatcaaa atactattaa ttttaatttt ttaggaatta acaaaaagcc ggactcatta
    30421 atcaattcta gcgagaggtt tagaagtata ttagaatatc atcctcaaaa aataaatctt
    30481 tctgtaagat atgaatttta taagcaaaaa atacttcaaa cattaatagt aggtaatata
    30541 gagcaaaaaa aattaaatca ttctttacaa ttttatattc caatagctaa aaagtggaat
    30601 ttacaatcta taagtgaata tacatatcaa tctaataaat cagtattcag atctagcact
    30661 tcttatagtt tcgctggata tacagaaaaa ttagaactac aatatttaga aaccaaatat
    30721 cagatacgtg taggacctag gtattcgtat gtattcgata aagagcaaaa tattaaaaat
    30781 aacaaaatag aattatacat agaaccacgt atcatttcga taaaagcagg aacatttatg
    30841 gggaaatttg gttggatata taatattgca aaaaatttgt cagatcctac tatggaatac
    30901 cttatgctag aaggatataa acaaggaaat aatggattga tccaaataca atggcaaaga
    30961 actttaaata aactaataca aatttctttg caatacgaag gtagaataat gcaacacagt
    31021 agcatgactc atgtaggcag catcactgta cgggctttat tataataaat tttataaaaa
    31081 attgttgttt aaatgcttgt taggaaattt atttaatttt gtcatataaa attaaatatt
    31141 tatctttgca taaaataaat cactttaatt gattttgcag aaaacaaaga gcttataatt
    31201 tttttaaaaa aatattacta aatcataaaa aaatatataa aataagaacc ttttaatatc
    31261 aagtttaaaa ttttaacttt aaaaaattat aaataattaa ttttaaaaat atatggattt
    31321 aaacgattta aaatttaatc agttagtact tattgctaaa aatctaaaaa ttagtaatcc
    31381 gaggaagtat aaaaaatcag aattgatttc tttaataaaa gataatcctg atttcaaaga
    31441 aaaagatata ttaaaaataa tagaacaact taacgaaaat gaaaaagata aagaaagtaa
    31501 ttttacagaa aataatgacg aacaaaatac taaaactaaa agaaaaagaa taaaaactac
    31561 taaagtcgat ataaacgaag atacaggtaa atctacacaa cctaaggcaa gtaatgttag
    31621 taaaaaagaa aaacaacaca cacaaagtgt tgataataaa aagacagata aagataataa
    31681 acctattgaa gaatcaaaaa taatcgatac agatgcatta gaaatttctg acttggcaga
    31741 tgatgaaatg gtaattgaca aattatataa ggatgaagcc acagatatag aaaaaaatgt
    31801 tgacagtgaa atggaaatag cagaagagag taatactaag acaaaaaaga tttatttttg
    31861 ggaaaatgaa ggtatcatag aagtagaagg tgtattagat atcttaccag aaggttttgg
    31921 ttttttgaga agttcggatt ttcattatta caattctcca gatgatgtat atgtaagcaa
    31981 tacactaatt agatctttag ggctaaagtt gggcgatact attgagggga ctataagacc
    32041 accaagggaa aaagaaaaat attttccatt agtaaaagta ttaaaagtaa acggcttaga
    32101 gcctcaatat gttaaagata gagtgccatt tgattatatg actccacttt ttccatataa
    32161 aaaatttaat ctaactggtc atcctcatga gactttaact agtcgcatca tagatttgtt
    32221 ttgcccgatt ggcaaagggc aacgagggct tattgtttct caaccaaaaa ctggaaaaac
    32281 tatgattttg aaagaaattg ctaatgcgat tgcatataat catccagaag cttatttaat
    32341 aattcttttg atagacgaaa ggcctgaaga agttacagac atgattagaa gtgttaaagc
    32401 tgaggtgata gcatctacct ttgatgagcc tgccgaaaaa caagtaaaaa tatcacaatt
    32461 agtattagaa aaagctaaac gaatggtaga gtgcggacac gatgtggtga tacttttaga
    32521 ctctattaca cgtttagctc gagcacataa tactatggcg cctcctagtg gtaaaatatt
    32581 atcaggtggt gtagaatcca atgcattgca aaaacccaaa agatttttcg gtgctgcccg
    32641 ccagatagaa aatggaggtt cacttactat tattgccact gccctcatcg atacaggttc
    32701 taaaatggat gaggtaatct tcgaagaatt caaagggact ggtaatatgg aattacaatt
    32761 agatcgcaaa ttggctaaca aacgtatttt tcctgcaata gatattgtgg cttctagtac
    32821 tagacgcgat gaccttctag ttcccaagga tatcttgcaa agagtttgga tactaagaaa
    32881 tcatctcgct gatatgaata ctcaggaagc tatgcttttt atacaagaga gaatgcgcta
    32941 tactcaaaat aatgaagaat ttttattaac tatgaatggc taaagcattc atctattaga
    33001 ttttaatagc ttctttaaca caacctcatt caatgccaaa attatttttg caagcacaca
    33061 cgtcttcatc atccattgtt gtaattaggc ccgcatgttg agtgttttgt gcattagcat
    33121 acttaataaa agtctgagca gaacttagat attgaatatc tctctgagta tctaataata
    33181 ataaataact aataataata ttacccatca tctctacaag tctgcgagca tgaaaatcta
    33241 ataattcttg atcagcagta tcttttattt tcgaataaac atgatcgtat tgagaagtca
    33301 tttttattaa tgaagacttc agattttgta attcagggtg cgtgatttga gaattataat
    33361 catttaattt ttcacgaaaa gtattattag taatgcctct gatagcggct actacttgta
    33421 gctgagaggt accttcatag atattagtaa ttcttgcatc tcgatagagt ctttcgatag
    33481 gatagtcttt catataacct gatccaccat gtatttgcat agcatcatat gtaacctgat
    33541 tagcatattc actagcaaaa agtttgacaa gaggagtgta atattcagat agtttttgat
    33601 atttttttag ttcatctctt tcattttgat tgagaggtct tttttcggaa agataattta
    33661 accctttata aatatcaaca aatctagtag tttcgtacat caatgttcga gctccatcta
    33721 gtttagcttt catcagattg agcatctcat aaacagcagg gaattgaata atgggcttac
    33781 caaattgata tctttctaga gcatatttat atccttctct ataagcagcc tcagctatac
    33841 caacggactg agctgctaca ccaagtctag cagcattcat tagagacatc acatatttga
    33901 taaggcctaa ttttctttgt ccaatgaggc gggcaggagc atttttgaaa actaattcac
    33961 aggtaggact accatgaatt cccattttat tttctattct tctaacagta acagccttat
    34021 cactacgatc atataaaaaa agtgaaaggc cacgtccatc agtggttcct tcttctgatc
    34081 ttgctaaaac gagagaaata tctgcatcac catttgtaat gaagcgcttt acaccattta
    34141 gataccattt ttcttttttc tcatcatagg tagcttttaa ttgtacggct tgcagatcgc
    34201 taccagcatc aggttctgtt aatgccatag ctccagtttt accttgagaa aaaagtggca
    34261 aatattgatc tttaatttct tcactagcaa attcatttat agtttcagca caatcttgca
    34321 aaccccatat atttgaaaaa cttgcatcag ctctagctat gatctcatta ctcattacaa
    34381 aaggtaatat tggaaaattt aagccttgat attttctcgg caaagtcatt cccatcacat
    34441 cagcctccat cagtgaatta tagttttctt gagtagcggg atgataatgt acttcattat
    34501 taattagttg tggaccttca gcatcaacta tttctgcatt gttggctatc acttcgccag
    34561 ctatttcacc aataatttct aacacttttt cataattatc tatggcatcc tctacgtctt
    34621 gaggtgcata atcataaata tctttttctg aaaaatcatc ttctttgata cgaactattt
    34681 tttccattag atgatgatta agatgaaatt taagggattt attatctaaa aagaaatttt
    34741 ccataacaca attatttaat attttgttta taaaatttaa tcattttagg tattactgaa
    34801 ttgacatctc ctattataac gtaatcagcg atttcattaa taggagcctt tggatcagta
    34861 tttattgaga taattgtagc actctctgcc ataccagccc tatgctgtac agcaccagaa
    34921 ataccacaag ctatatagag tttaggtcta actgtgactc ctgtttgtcc aatttgacgt
    34981 tctgactctg tgaatccagc atctacagct gctctcgaag cacctacttc tgctcctatg
    35041 agcttagcaa attcaaataa catttgaaaa ttttctttgc tacccatacc atagccacca
    35101 gctacaataa tgttagcatt tttaatatta attttctgag cttctacatg gcgctcgatg
    35161 atttgtaata taaaatgctc aggtttcaag attttaccga tatcgacttt actaatttta
    35221 gtattataag tagtagaaac tatttctttt ttcattacgc cttcgcgaac agtagccatc
    35281 tgtggtcggg tttcaggatt tatgatggta gctattatat taccgccaaa agctggccta
    35341 atctgaagta gcagattttt gtattctttt tgagttttag gctctacatg agaaccaata
    35401 gccagttctg tacaatctgc tgtaagtcca caacgtaatg cagatgccac tctaggagcc
    35461 aaatctctac caattggagt agcacctata atagctattt gaggtttttc gtttttaata
    35521 agatcgatca taatagctgc atgaggtaat gtttgataag gaaaaagcct gttatcttgg
    35581 gctaaatgta caacatctac accatagggt gataattgac tagcaatacc ttcatctaca
    35641 tcacctatta ctatagcctc taactgtaca tcaagttgat tagctaattt tctacctttt
    35701 gaacaaagtt ctaagctgac atcagctata tttttttctt ctgttaattc acaataaaca
    35761 aaaatactat tcataactct aagattttat ttgaagttta acctataata tggctattaa
    35821 tgagtgtttt cataaggttg tctatatcat tatcattatc agaaagtatt agattttctt
    35881 tagctttaaa gacaacattt tctatttttt taactttagt aggcgaacca ctgagcccta
    35941 accaattaac atcagcattg atatcttcta caccccactc atctattttc aaataaggtt
    36001 tattttcgaa tagttggtaa taatcttcat tacgttcttg gagttcagaa atagatgcag
    36061 ctcttttgta tttcattacc aatttagcat ttctaaatct acaacgtggg gcactttgat
    36121 tcactgtgat caaacatggc aatggtgctt ttatgatttc tatacctctg ccaagtctgc
    36181 gtttgatagt aatactcgaa tcatcaatat caataagctt ttcagcataa gttatttgag
    36241 ataatcctaa tttttcagca gtttgagggc ctacttgtgc agtatcaccg tctatagctt
    36301 gtctaccagt aaaaactaaa tctacaggcc aaagtttttt gatagccata gatagagcat
    36361 agctggtagc taatgtatca ctacctgcaa atcgcctatc agtcaataaa tatcccctat
    36421 ctgcaccacg aaataatgct tctcttatga tttcagtagc acgcacaggt cccatagtta
    36481 gaagtattac ttcacttcct ttaattttat ctttaacact caatgccatt tccaaagcat
    36541 gtaaatcatc tggattaaaa ataacgggta atacagcacg atttactgta ccatcctctt
    36601 tcatagcatc ttgcgttaca ttagcagtgt ctggcacttg cttagctaac acaacaattt
    36661 taaagctcat attattccaa ttttttcaat atttagtttg caaagataag attaaaaaat
    36721 aatataaaaa atttgaatct acttttaaca ataattataa ggtaaaaaaa ttaattaatt
    36781 tgttctaatt ttgtaataaa atgataatta tgaaaaaaaa tttttttgtt ctattttttt
    36841 tatttactat taaattattt tctcaaattg atgcattaca ttataatatt tacatagatt
    36901 ctattgattt tgatgatcaa ttaatttttt gtaaaaccac aataacatta gatgtaggaa
    36961 taaatgaaat ctctgaaatc agctttgatt tattgagctt agaaatagat tcagtgaaat
    37021 gtgaaaatac tttatgttta tataattaca atgacagcac actcaatatt agtggtttgt
    37081 ttgtagatac agtagatatt acgatttttt atcatggtca tcccacactc gatattggag
    37141 gttggggagg attttatttt tctggtgatt atgcatttaa tatgggtatt ggcatagaca
    37201 atataccaca taatatgggg cgttcatggt ttccatgcat tgataatttc actgacaaag
    37261 ccacttatga ctattacata accaccgaaa ttagcaaaaa agcagtatgt ggaggtattt
    37321 tgatagattc tacttttata aatttatcta ctatacaatg gcattggcac aatccacatc
    37381 ctatcccagc atatttagct tcagtttcag tgggtcctta tgtggtatgg aaggacacag
    37441 tacagggaat taataacatt gtaccaatag agatttatgt gcctaatagc tcattagcaa
    37501 aagtaccagg tagctttcaa aatttaaaat caatatttca tatttttgaa aaatattatg
    37561 gaccttacaa gtggagtcgt ataggttttg taggtgtacc ttttagtggg ggagccatgg
    37621 agcatgcaat gaatatagca tatcctatag cttgtataaa tggtaattta gcttatgaat
    37681 ctttatatgc acacgaatta tctcatcatt ggtttggtaa tttggcaacc tgtgaatctg
    37741 ctgaagaaat gtggataaat gaaggatggg caagattttc agaaatttta tacaaagaaa
    37801 aattgtatgg aaaagaagaa gcaaatttat taattaaaaa tttattatct gatgtattaa
    37861 aaaaggctca taaagtagat ggtggctttt atccactctg cgatatacct caaaatatta
    37921 cttatggcac cactgtgtat gataaaggtg ctctaatagt gaatacattg agaaattatt
    37981 tgggagattc attatttttc ggtgctacta aatatttttt agaacaattt gcatggtcta
    38041 atgcatcgtg tatagatata caggaaagtt tcgaacaata cacaaatatt gttttaaatg
    38101 attttttcca aaattatttg tatcattcag ggacgccaca ttacagaata gattctttca
    38161 atgtttcgca taataatgaa ataaatttta ttcaactcta tacttcacag cataggtatg
    38221 gaggggatta tttgacagaa catgattcta tcgaagtatg gctatgggga gcaaataaag
    38281 aaatggattc actgaaaata aataccggct tacaaaatta ttttattggt ttccatcatg
    38341 ctatagcacc aatagacgta tggatagatc ctaaacagaa aactgcagat gctattatta
    38401 atggttatgc ttttttgaaa tctatcagca atcattcaat ccccaatact aatgtaatta
    38461 ttacgcccac ttatttagaa gattcattac ttgtttttgt agaacatcat ttcataggac
    38521 cacatcaaat tttaaaaaat catcctattc ataggatatc ccctaatcat tactggacca
    38581 tacatttgcc atatttagat aataataagg taaatttaaa aatttcctat aatcgctctt
    38641 acccaataaa tatagatcca tctgattttt atttattacc tacggctgca tcagtagact
    38701 cattagtttt agtatataga aaaaacagta ctgaaccctg gcaaatcgtt aattttacta
    38761 aaactggtaa tcaagtggca ggatatctaa ccacagtaaa tgctaaagca ggagattatg
    38821 cttttgcaat aagtgagcca gggcagagca atattaatga ttttgataat actaattatt
    38881 tgcaaattta tcctaatcct actaatgatg aatttaaaat aatcgtcccc actagtggtt
    38941 ttattcagat ttattctatg ataggagatt taatttttga ggaattgata gaaactgaaa
    39001 taacttataa aattttagat aaaggtacat ttttagttag atttattccg tgtaataata
    39061 ctaaaacatt tcaaaaatta ctatttgtat attagtttat aaatttaaat atatctagca
    39121 aatgtaaaaa cataaataat tttatataaa gctataaaat caataaaaac ttatccttat
    39181 cataagtttt ttaaaaattt ttctacattt gtaaataaag caaatactac taagatgaaa
    39241 aaaaagttaa ttattttaat aataataatt agtttcaata atttatctgg ccaaataact
    39301 gaattagaaa aagagctcaa aaaaaagaat aaagatagtc taaatggttg gagttttaat
    39361 ggtttagctt ctattaattt ttctcaaaca gctctaatta attgggcatc tggcggtgag
    39421 aactctatat ctggtcatgg catttttaat attaatgcta attatttgaa aaataaaaat
    39481 tcttgggaca attcattaga ccttatgtat ggaatgttta ttgaaggcaa aaaaaagtta
    39541 aaaaaaactg acgacaaaat agaattattt tctaagtatg ggagagaatt ttctaaaaat
    39601 ctatatatat catcattact cgatttcaaa acacagatga cccctggcta caattatccg
    39661 aatgactctt tgataatttc ggattttatg tctccagcat atctatatct ggctgcagga
    39721 atcgattaca aaccatttga ttttctcact gcttttatat ctcctatcac aggaaaattt
    39781 acttttgtgt ataatcagtc attagccaat aatggagctt ttggagtaga accagctatt
    39841 tatgatgaac atggtaattt gctcaaaaaa ggcaaaaata gtagatcaga aataggtgga
    39901 tatataagac tagcttttga caaagaaata ctaacaaatg taacattaaa aagcaaaatt
    39961 gacttattct caaattatct atataaacct caaaacattg atattgattg ggaattatta
    40021 atatctatga aaatcaataa atatattaca gtttcgatat ctacacgttt gatatatgat
    40081 gatgatgtaa tgatagaaat agactctaat aatgatggta tagtagataa aaaaggtcca
    40141 agaatccaat ttaaagagat ctttggtatt ggattttcat ataaatttga taaaaagaga
    40201 gtaagtgtag attgaaatgt atactacaat agcataatta atttttcaaa aaatctttta
    40261 taattaaatt agtactaact ttactaaata aaaattaatt actatattaa aatttgttgc
    40321 ctcgaaatag ctataaaatt tatcaaaaaa cattattgat tcattgatta gcaatctatg
    40381 tatttataaa caagttgttt tatcattttt gttctttcag gttcaggagt atcagcgatt
    40441 ttttgactta gattttcatc attataagat tctaatttag agatctgatg ctcaataact
    40501 tttgcaggga aaacatcaaa tttattataa atgtcttttt gttcttttag tcttttacta
    40561 gattctacac atgaagtagg taaattaggt aattttttgg ctatatcttt gtattcctct
    40621 ttaaaaatat taccttctat tttatattta tcagctatat ctaaagcatt tggattattt
    40681 agaccttctc tgacagcgac agttaatcct gctattaata aataaatatc agcagatcca
    40741 tcagggacac gaaattcgaa agttggtttt tgagatatat ttactttttc aggatctttt
    40801 ccataattag tatttttaaa catttcaaca gcatttttgc tccatcccag cggcactctt
    40861 actaacgcac ttctattacg ttcgccccaa catattttag taggagcttc ttgatttggt
    40921 tctagacgaa ggtaacttgt tggtacacaa ttaccaaaag ctgtaagtga aggtgctagc
    40981 tccataatgc ctgcaattgc tcttttgctt atatcactta cttcaccatt ttgattaaaa
    41041 acgacatttt caccattttt taataatttc atatgtatat gcataccgct accagccttg
    41101 cctacagtga ttttaggggc aaaactcacc tgtacaccat atttataacc aagtactctc
    41161 aacacccact ttgctattat taactgaata gctgcatctt ctaatgcaac aggttgaaat
    41221 tcgatttcat tttgttcata agaataagta tcatctgaaa aattacccac ttcactatga
    41281 gcatatttaa tagatgcacc cattattttt agacaattta atgcttcacg acgcaaattt
    41341 tcaaatttca caaaaggtac agactcatga taacccctct gatcagtagc taaataaaga
    41401 ggatcttttg gaaatattac ataatattct aattcaccca tcccctgcat ctctatgcca
    41461 gtttctttta ctaaagattc ttcagctttt attaaaatat tttctggaga aaatagtgtg
    41521 ggattaccat ctttatcaaa atatttacaa aagaaaccta aagtaggatc tacctctaat
    41581 ggattcaaaa atgcagagtg cagctttggt atgacatata aatcgcttga tccagtttct
    41641 aaatatttga aaagactaga accatctacc ctctctcctg tagataaaat ctctcttaag
    41701 tgatcttcgc cttgtatgat aaaatttaat gttttgactc tgccatcatc tccaatataa
    41761 tgaaaattta aatgatttat atcaaacttt tttacatatt tcactatatc ctctacttga
    41821 taatcttgct tatctttgtc tataaaatct tgtaatggta ttaattcttc aaaatacata
    41881 atatcttttt tgcaaagata tataattatt tattataagg ccttacttaa aataaaattt
    41941 agagttattt accaatataa ttttactcta ttccatagtc tcgacatgct cctttgatat
    42001 atattgaaca aatttatcta gtaatcattt aaaataaaaa aaaagtttct atatttgcca
    42061 aatcaattaa tttttttatt aaataaactg agaattatta gctatgaatg caaaatattt
    42121 gtttttgatt tttttaaatt tatccctttt aaatttttct atatctgcac aaattttagt
    42181 aaaaataggc attagaaatg atttaaaaaa cattaaaatc aaaatctcag agggtgatta
    42241 tgcccttact aatggtaatg atagtattat tttaaagaaa aatgacacca ttattttaga
    42301 aaataattat aataaactaa gtgcgagaac tggcataaaa aatataaaag gcgatagtat
    42361 cttattaata agcacaagta caaatggaat attttctaca attattaata acaaattaat
    42421 agaatatgaa gacaacgcta tattttatcc taaaaataat acaagtatta attttataaa
    42481 tcaattagac atagacaaat atgtagcctc agtgatatta tcagaaacat atcctaattt
    42541 accttatgaa ttttataaag ctaaatcaat agttgtacgc acatatacca catatatgat
    42601 aaatatagaa aaaaagcata taaatgataa atatgacttg tgctgtacta cacattgcca
    42661 attatataag gggaaatgca acaaccagac aataatctct gcctgcaaag aaactaaagg
    42721 aatgatttta gtagataatg ataatttgcc gatattagca gtatatcact ctaatagtgg
    42781 aggtctaaca aatagttcag aaaattattg gggcaaatca ctaccatatc tcattaccat
    42841 aaaagatgaa tatagtacca aatctaaaaa tagtacttgg acatatacca tatcaaaaga
    42901 tcaatggttg tcgtatctag ctaaacatgg tatagattct acaaataaaa attttgaaaa
    42961 tgccacaaat ttttatcaac ctattagaaa aaaatatttt atagatactt taaccttaag
    43021 aaaaatacga gaagatttta atttaaaatc tacgtatttt aatatacaag atgagggcga
    43081 caaagttatt ttcaaaggac gaggctatgg acatggagta ggactgagtc aagaaggagc
    43141 aatagaaatg gcaaaggaag ggaaaaattt taatgaaatt ttagaatttt attatccaaa
    43201 tacaaaaata tcatatttta atctttgagc tatatgaatt gggcaaaagc acctattgtc
    43261 cgtattacat tattttatat tattggtatt attatttaca atcatttacc atctcataat
    43321 tttttttata tagttacact acttacaacg tttttactag tactctcatt tggattttat
    43381 ttttattatt ctaataaaat atcattacat acttggtatc ttgcactcac tatattttta
    43441 tcttttattg cattaggata tttcagaagc tacacattcg atgaaagaac aaaagaagat
    43501 ttttatgcga atcatttgga taaaacctca tattattttg gcgatataac agatataaaa
    43561 gcctcatcac agacgagaac taaagctact gtaaatttaa aaaaaatgct aatagactct
    43621 cagtgggtaa atactaatgg taaggtatta atatatctaa atttaccaat cgatagtttg
    43681 caagataatg atcattctat aatttttata aattcactac aatcacctaa tttacctaaa
    43741 tatccttatg aatttaacta tgcacagtat ttagcaaatc agcaagtcta tcatacaggc
    43801 tttataaacg attatgattt ttttcttcta cctagagaaa gtacattttt agaacaactc
    43861 tctcgagtac gcaataagat agtagaaatc atatatacta atatatcaga tcccgcatca
    43921 gcaaatttaa tagctgcaat gcttttaggt tataaagatg ctatggatgc agagataatt
    43981 aaagactttc aacaagcagg cgtagtacat ctattagctc tatcaggact acatgtaggc
    44041 atcatttatg gtggaatata tttattatta tcattcatta ttccttttaa atatcgaaac
    44101 aaaatcacaa ttccattagc acttattact ctatggctat atacattaat ggtaggagct
    44161 atgccgtcag tagtacgagc cgctattatg cttacattgt tacaaatagg tgtacttttt
    44221 aatagaccag ttaataatta caattcatta gctacagctg cattgtttat tttaattgct
    44281 aatccattac aattatattc tttggggttc cagctttcat ttacagcagt attgggcata
    44341 ttttttttca atgcaccatt aatgaatatt tgtaaaacaa ataaaaaatg gctaaaatgg
    44401 gtgataggct ctttatcagt tacaatggcc gcacaattag gcaccttgcc ccttactatt
    44461 tattattttc atcaatttcc tacttatttt ttaatagcta atttaattgc agtgcctatg
    44521 gcatcaattc taatatatat aggttttttt gcgattattt tatcatttat tccttttgtt
    44581 ggattattaa taaaaaaaat atttgctcta ttagtcaagt tgtttatact ttttatacgc
    44641 tttatatctg atttgccata ttctacttta tcaaatatat acatagatcc attgtcagct
    44701 tttttgattg gaataataat tattttaata gttttgcaga tttattataa aaatattaaa
    44761 atatatccat cattgataat tacagtaatt ttgtttggca ttaactataa tctctcaata
    44821 gctaagcgca ttagagaaaa tccatctttt gagatattaa ataataaata tgctgttata
    44881 ggtcaaaaag aacaagaaaa atttcattat ttaatcataa aaccagattc agcacagctg
    44941 cctgacatta atacaaaaat tgacatgttt tgtaattatt attattgtaa aaataataat
    45001 agctatgatt tgcaagataa agtaaaatcc tcagcctttt atttgaataa tccagttata
    45061 gctaacaaag atcaaacttt tgtggttggt gataaaaaag aaatcttaca ttggaaggaa
    45121 ctaactaaat taccatatat agaaataggg tggtatcgat tgagatatta tgatataata
    45181 gatgaatagg atagtacaaa tttgcacttt tatatgtaaa tcaatgaagt ttgggataaa
    45241 atacaaagat gaaaacagaa ccaaataaaa agatggtgta tttggaattt tgtattatat
    45301 atatttaact ttgtaagttg ttagttgtga ttttaaaaaa ttacttatgc aaaaaaatat
    45361 taaaagacgt ttccctccag actttacttt aatttttata tatcttatct tatctatcat
    45421 tggattagta aatatttatt cagcttctta tcaagatggt atcagttttt ttagtttttc
    45481 atttcaatat gtacatgtaa agcaatttgt atggatatta acgggatgga ttttatttat
    45541 attaattatt tattctaatc cacatttatt ttctaatgct gccgagatat tttatgctat
    45601 tgtaatatta ttactttttt tagttttagt cataggagct attgtagggg gcaataaagc
    45661 ctggttagat ctaggcaaag gtatcagact acaaccatcc gaatttgcta aatatactac
    45721 ggcattatta atatctaaga tatggagcta tccagatgct aaaataaata taaataatac
    45781 tattagaaaa gtattgcttg taatatttat accgatccta cttgtttttt ttcaaaaaga
    45841 tgtaggcact atgctagtat tttttgcttt tatctttgct ctctatcgcg aaggattacc
    45901 tacatatgtt atttttattg gtttttggtt tattatagtt tttatccttt catttttagt
    45961 taaacaattt acatttttat tgattttaac tggtttaacc ttggcattaa tatttatttt
    46021 tagaaagaaa aaaaatatta taataaaatt tgttcaggtt tatgtattaa gtatagtttt
    46081 aataattggt gttaattatt tctataatat gctaatgcca caccatcaag agcgaataga
    46141 ggtaatgcta gggctcaaag atgatcccaa gggggcatca tggaatttgc gacaatctat
    46201 gatagcaata ggttctggtg gatttacagg taaaggtttt ttgcaaggca cacaaacaca
    46261 aatgaaattt gtccctgagc aaactaccga ttttattttt tgcgctattg gtgaagaatg
    46321 gggctttgtc ggtgcttttg ttgtgatagc tctttattta ttttttttat tgcgtataat
    46381 tattttagcc gaaagacaaa gatctatttt ttctagagta tatggctatg ccatagcatc
    46441 aatttttttc actcacattg ttattaatat tggtatgact gtcggtttga taccagtcat
    46501 cggtattcca cttccctttc tgagctacgg cggatcatcc tactgggcat ttactttatt
    46561 attagctaca tttatcaatc tagatatgta tcataatgag attgtttgat cgtataagtg
    46621 tcttatgcta taactcttac aaactcagtc aaaagcttac ctaggcaatt cagagcattg
    46681 aaatgttatt aatgcactca atataaaaaa tagaacaact gttttttttt aatattcttc
    46741 tactttcaat tgataaaaac ttctaggatg cgaacaggct ggacatactt gaggtggttc
    46801 tgtgcctaca tgtacataac cgcactctct acaaaaccaa ctcacaggta tatctttttt
    46861 gaaaaaagta ccagcctcta cttgattcaa tattttagca taacgttcag catgatgttt
    46921 ttcggctaca gcaatagctc taagcctaat agctatagca gagtaacctt ctttatcagc
    46981 tatattagca tactctgggt acatactatt atattcatat tcttcacctg caatagcaga
    47041 ttttagatta tcaatagtag agccaagaat tgtaatagca ttagcttcaa ccgtaatttc
    47101 atcaatattt tcattagttt cttgtttgag ctcatttatc attttaaaaa gccaactcgc
    47161 atgttctctt tcattatcag cagtcatagt aaaaatctca cttatttgct cataaccttc
    47221 atttttagct atttttgcat aaaaagtata acgatttctt gcctgacttt cgcctataaa
    47281 agcttttact aaatttttga ttgtttcttt catatttttg tatttattgc tattgtatta
    47341 aataattcta tcaggatttt cttcaataaa ttttaaccat ttattttttt taccattcat
    47401 catgttttca ggtataatag acgaatatgt agaataataa tgacagagtg cgatggcagc
    47461 ggcatcagta gcatcaaaag ataaagaatt attttctaat tttaaaatat tatttaccat
    47521 acctgctact tgttcttttg aggctctacc attgccagta acagagattt tcactctacg
    47581 aggtgcatat tcatatacat ctataccata ctctaatgct gcagctatta ctactccttg
    47641 agcacgtcct aactttaaca tagattgtac atttttacca taatatggag cttctattgc
    47701 taaaacattt ggattgtaag aaataattaa ttcttttaca tttctatgaa ttaattttat
    47761 cctttcaaaa tgatctttta cacttttaac tgatatacat cctatatcta aagttattaa
    47821 tttatcatca tcaatgccta taattgcata acctgttaca tttgagccag gatctattcc
    47881 caatatttta taataatcat ttaaactcac tatacttttt tataaaatta gataaaataa
    47941 tttaaaataa aaaatattat tgattgatag aataaaattt taattatttc ttactttttt
    48001 actcaaatat tatcagctat ctctggaggt ggagtatgtc tagttctcat ctgcatgaca
    48061 aatttcaatt cccatctacg cgatttatag tatgagtacg aaagtatcaa accaacaaac
    48121 catcctatag gtattcctaa ccaaatacct agtgtaccta aatgcttcga tagcatatac
    48181 gaaaagggaa tgcgtactat ccaaatagca gctaatgtga tcaacatact tatcatggta
    48241 tctccgctac ctctgaatac tgattgataa gcaaacatca ttgcatataa tacataaaag
    48301 taattaagcg ttctcaaata ctcactacct atttctatta cagctatatc tttggtaaat
    48361 aagctcatga tagttttact aaaaataata atcaaaacag aaataataat agtcatagaa
    48421 actgttatgc gtaaagtagc ttttaagccg ttttttattc ttttaaattc taatgcccct
    48481 gcattctgtg ctacaaaatt ggctaacgcc gatgaaaata taaatgctgg tatactagct
    48541 atagcatcta tcctagtagc tactgaataa ccagctatag catcagtacc aaaagatgca
    48601 actaagctca tcaaaaccac agcattaata gcaattacaa attgttgaat accacttggc
    48661 aaactaattt tcataatata tttgaaaatt ttcgaatcaa acttcatttc ttttaatttg
    48721 atagaaatga aagggtgctt tttgtttata taaactatca ttaagattag agctaaaatc
    48781 tgtcctatca cacttgccca agccgtagaa gttagcccat aatttaaaac aacaacaaaa
    48841 acataattga gtacaaaatt gaccacaata ctaaatacta agaaataaac aggtgttata
    48901 gaatctccta taccacgcaa aaccgcatta gttgcattaa atggaaaggc aaaaagaaat
    48961 cccaaacaaa atacattaaa atacgatgtg gcttgggcta taatatcttc ttgagctcct
    49021 aataatttta ataaatattt acctgttaat atagaaaacg tagtcattat tatagcaaaa
    49081 actactaaag atatataact agtgctaata ctttttttca aattatcata ttgtttacta
    49141 ccaaaatatt gaccaataac aatagacaat ccaatagtaa aaccaataat caatgatgtc
    49201 acagcaaaaa taataggaaa agatatacct actgccgcca aggcttcttt acccaaataa
    49261 ttacctacta ttacactatt tattagtgaa tacaaatttt gtaatacact acctacgatg
    49321 agtggtaccg catattttac tatcgttttt cgttcgtcac cttttgttag atctaacatt
    49381 ttagccatat gtacagtgtt tttagtataa atttattaaa ttaatattcc ataaataaga
    49441 attaacaatc aagtaatcac taaattgtta tattcattca tagctttatc gattccttcg
    49501 aatgcccatg tataaatagc atcagaagct ataggaaaaa ccttattaat tatttcccat
    49561 tctctaaggg gccatttttc taaaacataa tctatttgtt tacctggagt gaatttatca
    49621 tcaataccaa tgcgcattcg tgggatttgc gtagtatgca aaacatcaat tatgttagct
    49681 aagcccttgt gagagcccgc actgccctta gctttgatac gtattttacc taacggtaat
    49741 tctatatcat ccaaaataat cattgtatta tctagaggta tttcaagcat ttttacccaa
    49801 taatcaactg ctctaccact tagattcata tatgtagtgg gttttataca aataaaaaat
    49861 ttatttttta tctttatatc agctctatat gcatatctat cttgcttaaa agctgaggaa
    49921 tattttttta caaaataatc taatacatca aaacctacat tatgtctagt gtgctcgtat
    49981 tcataaccta tattacctag acctactaat agaaatacat tatctctcat taatgtttat
    50041 agtattaatg gaaatctttc gtttcaatta atttaccatt ttcatcataa aaataccatt
    50101 tgccatatgg cttaccccct ttatagctgc cttttatgcg tagattgcca ttttcaaagt
    50161 aaaccagact ttttccttca cgtgtcccat tattatattc gccttcagaa catagttttc
    50221 cgtttgggtg ataatatttc catataccat gtctcaagcc gttatttaac ccacctacat
    50281 attttagttt accattagga tagtattctt ctacttttac tttattatta tttttatcta
    50341 aataatacac aattttagga ttaccatcag ggtaaaactc ttcaattact tgggttaaat
    50401 cttttttaca tccagtaaaa gagattatag taataaaaac aaaaataaat tttgttaata
    50461 ttttcatttt ttcagtcttt tatttaaaat ttttaatact tcattagtaa tatctaaact
    50521 aggattagca taaaaaatag caccttcatg tgaataaccg agtacataat caaattccaa
    50581 ttctgcaggt aagtatttta tagcattttt gatactgtct aaaatttcag attgcatagt
    50641 catttgtaaa ttttgaattt gaatatcata ttgatctctg atagacatga gttgttgttg
    50701 ttcttttacg agttgttctt ctcgtctacc agcctcatct ttggtaatta ttttacctgc
    50761 ctgaatatct ttttgatatg cctccatgtt tttttgaagt ctactttgcc tatttattag
    50821 atctgtttcc aaagaagtaa tttgtctttg taatctattt tgtagggtat ccactaaggg
    50881 atatttagtc ataatagtat ctatatttat atgtgctatt ttgccattta cataaccagt
    50941 agatacaggg gctgatattt ttttagtagg gacaaattga ataatcaaca aaaataatac
    51001 caatatagaa agtataacgc accaccatat aaaaaaatta ttatattttt tagtatatgc
    51061 attgttgttt gttaaattac tatctatact ttcagatggt gtagatgatt cataattcgg
    51121 atctaaattt ttttcttctt ccataattaa tttatttttt taaaacgtta aaaagtcatt
    51181 tttattttaa ttacccattt cagaaataaa tttaatacgc attaaacgta cttcctcttc
    51241 tgagtattca ttttcaccta attcttctaa tgcttgttta atgtcatctg tgctagcaga
    51301 tctaaagtac tcatatattt cttcttgatg ataaggatct atgacatcgt ctatgaagta
    51361 atcaatatca atacgaatac cacttgctac tattttgtct atttgatcta gtagttcatc
    51421 aaaatttaga tttttagcat atgctatttc atctagtggt attttgcgat caatactttg
    51481 aataataaaa atttttaaat ttgaagcatt aggggaggtg cgaattataa tatcattagg
    51541 acgttctatt tcattttctt ctacatagcg ttttataagg tctacaaatg gttgaccata
    51601 cttttgagct ttaccgacac caacgccagt aatattttgc aactcatcta atgtaatagg
    51661 atattgaatg gtcatttctt ctaatgaagg atcttgaaag atgacaaaag gtggtagatt
    51721 atgttgcttt gcaatcttat tgcgaaggtc ttttaacatt ttgagcaaaa tagcatctgc
    51781 tccggaccca cctttcattt ttatttcatc atctaattct tcattacctt ctgaaaagtc
    51841 tctatctctt gttagcataa tacttttcgg ttttgttaaa aattctcttc ctttttctgt
    51901 tatttttaag acaccataat tattgatatc tttctctaaa aaattcaaaa ttaatatttg
    51961 tctaataatt gaattccaaa atttttcatc ataatcagca ccagcaccat aacttttaag
    52021 tttttcagct ttaaattgtt ttaaagtagc tttatctttt tctttaccta atagtatttt
    52081 aacaatcatt ttagctttat atagttcttt agtttcatct ataacattta aaactgtcaa
    52141 aacttcattt ttagcatcaa attgcatttt aggatataaa caattatcac aacagtgaca
    52201 attatcttct gtgtaatctt caccgaaata gtgtaaaagt aatttgcgtc tacagatagt
    52261 actttcagca taggagatag tttcatacat tatgagacct gcaatttctt tttctgctac
    52321 accacgattg ttctgttcca tcttatcaaa tctaattaga tcatcctcgt tatagaaagc
    52381 gatgcagtca ccttctccac cgtctctacc ggcacgacct gtctcttgat aataagcctc
    52441 taaactttta ggcatatcat aatgtattac gtatctaata tctggcttat caattcccat
    52501 accaaaagca atagtagcta caataatatc tatctcttgt ctgagaaaaa gatcctgagt
    52561 ttgtcgtcta atagcaccac tgagaccagc atgatagggt aaggcattga aaccattagc
    52621 ttgtaaaaat tctgcaattt tttcagtagt tttcctcatc atacagtaaa ttatgccgct
    52681 tttcccttcc ctttctctta ttatttgtat caattccttg ttaatatttt tagttttagg
    52741 ccttacttca taataaagat ttggtctatt aaaactagaa ataaaaatat tgggattctt
    52801 caattttaaa tttttaatga tatcttcttg caccttagga gtagctgtag cagttagtga
    52861 tattactggc acatcttgcc caatagattc tatcataggt ctaatcatac gatactctgg
    52921 tctaaagtcg tgtccccatt cagaaataca atgcgcctca tctacagcat aaaaagaaat
    52981 ttttaaattt ttaaaaaaat ctatatatga ttcttttttt aaagactctg gagctataaa
    53041 aagcattttt gttttgccat ttgctaaatc atcaaaaatt tgttttagct ctctacgagg
    53101 cagagtacta tttagcatat gagctacttt gtcatctgta tgtatatttc taataatgtc
    53161 tacttgattt ttcatcaagg ctaataatgg tgaaataatg atcgctgtac catcacacaa
    53221 aagtgcaggt aattgatatg ttagagattt ccctcctcct gtaggcatca ctacaaatgt
    53281 atctctaccc gcgagtagac tacagataat ttgctcttga tatagtttga aattatcata
    53341 accaaagaca gactttagta tataataaat ctcttctctc gtaatattgc ttgttccaat
    53401 agtttcagca ttcattacaa tccttatttt tatattttgt tttttgtaaa gataatcaat
    53461 tttttacaaa ttaaaaataa atttaaattt tcatcaatta ctatgattta gatagattat
    53521 attttattat agccatatta tacatagatg tagacataaa gcacaatata catatattta
    53581 gttattaaat ataagcaaat ttattaataa actttggttc tataacgttt attgtgggca
    53641 ataaaatttt agatcgttac aaaaaaataa taatattata agaaaaataa atattttttg
    53701 gttttaaaaa ttt
//
LOCUS       DAOPRN010000007        47019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_565_length_47019_cov_128.209160, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000007 DAOPRN010000000
VERSION     DAOPRN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 47019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47019
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_565_length_47019_cov_128.209160"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(444..1874)
                     /locus_tag="PKI65_01585"
     CDS             complement(444..1874)
                     /locus_tag="PKI65_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOB77165.1"
                     /translation="MKTKVLTLVTVLILIITSVNAQKVEKNLGEIEKLNLTGGLYILK
                     QGQGKLIYEANADNNIAILYDSINKILDIETVNKMEIYLPNIKELNLTLASRLTCNDT
                     FQTNYLKITANEASMCEINVLCDTLELVAEEASYINVKGECNYLNIKASEVSYINATD
                     INAKTVYVEANEVSKVKVKATDTLEIISNNASFVTYYGTPKYVNIEDDSFIKNKYIKQ
                     YDTLDKFDKKISFEFPFSDFIFDDENNEEDSDTKNQIQDKSDKKTKQSNKKVEFSGHW
                     ASLDFLFTNYADPSLSYSLPPNYDFLDIYFPKSSGVQFNFFQQSIPFNKNGTLGMVTG
                     LGIQWVNYRFGQKPIITDTKNGISFLIDTAKNVKKSKLTASYLQLPLLFEYQAKTKPY
                     FHISVGAIGGVLIGSHTKIVYSSPDKVEKENGINSLYPFKYGVVCRFGIKHLSFTVQY
                     NFSPLFKENKGPEVMPLEFGVTLISF"
     gene            complement(2404..3363)
                     /locus_tag="PKI65_01590"
     CDS             complement(2404..3363)
                     /locus_tag="PKI65_01590"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77166.1"
                     /translation="MSKEENKEFLDNLYSLMKKLEADVVDYQGLKILSAPPLCPDCRS
                     NHIIKNGLQNGMQNYCCKDCGRQFRVTTGTFVYRLQKKEKMLDYIRCMVAGKSLRACA
                     KEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSEKGRIYKNAEELKR
                     ARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMPKDGVMCSSNKKAF
                     KKVRRNVIKHKEIVTSNRQKRGIYSLSTVKKKASEFTNWIYFKFRGVATKYLQNYIMW
                     YVVMGKYLTGKTISNTGRMLNLASSDRWAWYRYRELVNITYVD"
     gene            complement(3513..4583)
                     /locus_tag="PKI65_01595"
     CDS             complement(3513..4583)
                     /locus_tag="PKI65_01595"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOB77167.1"
                     /translation="MRIGNKNPIYVEENLLNIFKFLRYHIGTHQKYKCLILADELVAS
                     IYENELNYLINYPYFNHVRLLKYNVDENVKNIDNIKNIWDTLSNDKFNKKDLLISIGG
                     GTISDIAGFASATFKRGINLVIIPTTLLAMTDASIGGKNAINYKNIKNEIGTYYSPLF
                     TYIYPNFLNSLPEIEILSGFTEIIKHALLNNKDSVREILNLDPRKLPPISILKKSIQF
                     KHHIVRIDPNEKNRRKILNLGHTLGHCFESIRFSNHLPTSHGICIAWGLQYTIKISEY
                     LLNFPPYWSKKIIEWLQTWYGKPPKIKFNDLLPYLLQDKKNSDDKITFILLYAPGKPK
                     IHKFNIQELKKIYDKCFHDFAS"
     gene            complement(4640..5638)
                     /locus_tag="PKI65_01600"
     CDS             complement(4640..5638)
                     /locus_tag="PKI65_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOB77168.1"
                     /translation="MNNLVFEYPYVLLLLILIPILWWWNVKKRSYHKYNFIKWPHIHA
                     LPLKTKGIRTFLFNSLPYLRLLALAFIIIAMSRPQLKNVSSSVEHEGIDIVLVNDISG
                     SMLAQDFIPNRLEAAKNIAEDFINKRPNDRIGLVAFSGSAYTACPITYDHEILKYQLK
                     QLRTGIITDGTAIGDGIMVAISWLQKSDANSKVMILLTDGVNNAGYVDPVTAAQIAQT
                     MGIHIYTIGVGTRGKAPYPFQDPFGRIVYDYVEVLIDEDLLINIANNTDGKYFRATDN
                     ESLKQIYEEIDQLEKTRFHYSTIVSHQDIFDIPLFIALICLLLEGILRWGIIKQRV"
     gene            complement(5631..6602)
                     /locus_tag="PKI65_01605"
     CDS             complement(5631..6602)
                     /locus_tag="PKI65_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77169.1"
                     /translation="MFRSNFSYKMYTFIIIILSLTTLLNAQDIDIFQRVEISKDTIYI
                     GELANIKIIINSQNTQIDSISSKFIKKTPKQLYISIDHPWIKTNNHTWEKRYSITAFD
                     SGNFFLPQLNIKLWYKNKQKLTNLIIDSIGFNVVPMPIDTSKAYKPIKNIYDISQPPL
                     KSKWWLWLIISISAIILILIIILIYRNNKIRKEQKSLYYNPELTPIEEAMQALKQLKE
                     NNSIAQMNAKEYYTILTDTLRRFILRIYNIKTFEMLSSEIFLEMHKYCNSTEWLAKLQ
                     QFLNTSDLTKFAKYTPTLEERYRDYEFCNQFINYLWEQEQLKKEQYE"
     gene            complement(6737..7621)
                     /locus_tag="PKI65_01610"
     CDS             complement(6737..7621)
                     /locus_tag="PKI65_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOB77170.1"
                     /translation="MSLSVEDLRKKVRYISITARKLSNQMFAGQYQSAFKGKGMAYAD
                     LREYVYGDDIRDIEWNVTARHNHPYIKVYQEERELTLILMIDVSRSTIFGSTGKMKNE
                     LISEIAGILAFSALKNNDRIGVILFSEVIEKFIPPKKGLSHILTIIREILAYEPENPK
                     TNIKKALEYLNHVIPKRSIVFLISDFYDTQYELAMGITNKKHDFIAMQIVDRLEITPP
                     TEGFVITCDAETGQFVPLDFNNPKTVTAWKDVYQVHYNYLEKTCKKHNIDLLSIDTEI
                     DYIPLLSALFKKREKRLV"
     gene            complement(7618..8613)
                     /locus_tag="PKI65_01615"
     CDS             complement(7618..8613)
                     /locus_tag="PKI65_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOB77171.1"
                     /translation="MDNTIDIASLNQKIEEASRFIDLIQMELSKVIIGQKHMTNCLIM
                     ALLTKGHILLEGVPGLAKTLSIKSLASCIQGDFSRIQFTPDLLPADLIGTMIFSPRSE
                     EFQVRKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGNNTYPLPDPFIVMATE
                     NPLEQEGTYPLPEAQVDRFMMKVLVDYPQKNELKYIIELNLQEKFPTINAVATPKDIK
                     NAQKLVLEIYMDEKIRNYIIDINYATIQPEAYRLPQYKNMVRYGASPRGGIYMSLAAK
                     AYAFIHHRGYVIPEDVRAVAFDVLRHRIGMTYEAEAENITTDQFIADILNVIEVP"
     gene            complement(8873..10780)
                     /locus_tag="PKI65_01620"
     CDS             complement(8873..10780)
                     /locus_tag="PKI65_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOB77172.1"
                     /translation="MGKEEKKNELSLNMSENNNAIDEFLPTDVNNDIELKYQIDEVNE
                     EEDDDIEVNEEEKEKYDHLDLDQINEEFAIVLENNDLSYIRPRVLRLYDLHQLIYENE
                     LKNLSNIPVEKDRQKIEENLKTKKETFDQLWKQYLGKKKSLKDQYIKSLEENYEKKLA
                     ILEQLKNIIDNNEIPLKEAFDHFNQIQEEWKAIGAVPHNKANDLWMRFNHYVDLFYKK
                     VELLKEMIEIDQRKNLEAKIELCEKAESLLMDETISPKDAYRQIYELFDQWKQIGPVP
                     KDKKDEIWERFQTTLDKIKDNYRNSIELQKQRRENNLEAKKAIIDKMKYILSEEIVTH
                     DDWQKKTNEVQDLFNVWRSIGPVPKEYNESIWVEFKSLMDKFYQNKKEYYEILKIEWQ
                     ENYNKKLSIIKQTEKLVNSNNWKKDTQTILKLQEEWKKIGFIPAEKNEYLWHRFRSAC
                     DQFFANKEMYYANLATAEVENLYKKEAILKELDDFQFNNNPKEDLERLKAIQRQWYEI
                     GNVPYKERKRIQDTFRQKINSLFDQLKGVRIDESNDEFKQRIEKILEHSGGLKEAKSE
                     LYNLNNKIKKLRSDIQTWENNLSFFKSSANAELLLQEYKKKIDNAKTDLKILEEKAKY
                     LRQAVNESEKN"
     gene            complement(11114..11590)
                     /locus_tag="PKI65_01625"
     CDS             complement(11114..11590)
                     /locus_tag="PKI65_01625"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOB77173.1"
                     /translation="MKKIEKELWTKKDDFCFGCSEKNPIGLKLDFYDMGDYIETQWLP
                     NKNYEGWQGVLHGGIIATLLDEIGGWVVVSKIGIAGMTVELNVRYHKQISSNQLLTLR
                     GKLVDVKKNIAFIQAEIIQNDIVKASGISKYFLFDQKTSREQYQVINNNLKSIENN"
     gene            complement(11587..12336)
                     /locus_tag="PKI65_01630"
     CDS             complement(11587..12336)
                     /locus_tag="PKI65_01630"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOB77174.1"
                     /translation="MFSLSWKKLIIYIILIISTVLSIISISSANNILQSVLLPNSDSL
                     FFTLLLHPFIHVNWWELILNILMLYLCARFFISKLNWWELVILWIIASVGGGFIANQW
                     SYNLQNWGGLTLISTALLSWSTIANIGILLTFKNKKIKLNILSNICVLIYLVIILLFN
                     RDLILSFIIVSLSGAILGCISYKNPRYISNNPAILNDIPPINDYDYYDRQKEFENEIN
                     RILEKIKNEGETSLTNTEKEFLDEYAKRRVK"
     gene            complement(12351..13172)
                     /locus_tag="PKI65_01635"
     CDS             complement(12351..13172)
                     /locus_tag="PKI65_01635"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOB77175.1"
                     /translation="MNDQRLFGPSHFSLFPPAVLNLLIINTIVYFGTVVFMQRGIDII
                     NILGLHYPASPYFRWYQFITYMFMHGSFMHLFSNMFALWMFGYMLENVWGTKRFLTFY
                     FVTGIGAALFQIGVNFFEFTSMQKAIAAYSQSLDPNEFVYILNKFFANRYDPVKVNEL
                     LNNWALVAPSTVESLKGLLYMMMNIPMVGASGAVFGILLAFGLLFPNVPVFIYGLLPV
                     RAIVFVLIYGAFEFFAGVYQPHSGIAHFAHLGGMIFGLILMIIWGYRFKPYKFYY"
     gene            complement(13165..14970)
                     /gene="mutL"
                     /locus_tag="PKI65_01640"
     CDS             complement(13165..14970)
                     /gene="mutL"
                     /locus_tag="PKI65_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOB77176.1"
                     /translation="MNEIKRGIIILKEQIINQIAAGEVVQKPASVLKELSENSIDANA
                     SSIDVIIQNAGKTLIQVVDNGQGIYPVDISLAFERHATSKIYNIEDLYSLYSYGFRGE
                     ALASIAAVSRVEMFTRTADLELGTHCLVENSKIIKIEPTACPVGTNILVKDLFFSVPA
                     RRNFLKSDNTEMRNIWDEFYRIAIPNNNIAFSLTENDELKIKLPPTSLKQRIIDIFGK
                     NYESKLIYYEEEFPFGKIMGYISTPNAAKKTRGNQYFYANGRYFRSPYFHQVIIRAYE
                     GLIEKDAIPAYVIFFDIPPQRLDVNVHPSKTEIKFQDERLIAASLNSLCKKALGISGL
                     TPGIDFDAIPTHIFSINSPHTDKSYSNINSQFPHKPLSDTSKLFYQKINTQKEIINEN
                     YRELFEEISMIEKELEEKNENNKDVVLSSDNKILITKMKDGILIGDVESILQRLIYDQ
                     LITNKNNINAGCQHVLFPVIINIEPKYMGTFLSLQSLLEEIGFKFEKISNNQFQVIGI
                     PCHGYIDEDNIESFIYRLLELPEAGLPLNNELLFYNMALNAAKKQLNKLNSESLNPLL
                     ANLFSLTDPQKAIGGKNNFIILSTNKLLQLIQSYE"
     gene            complement(14987..15226)
                     /locus_tag="PKI65_01645"
     CDS             complement(14987..15226)
                     /locus_tag="PKI65_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77177.1"
                     /translation="MSFLFPTPKPKKFEYKPRFSSKEDIDLSDLDERELERERLRREL
                     DQKWKRKIKARKRTSINIWSALIVLLLLLLVIIFL"
     gene            15359..16711
                     /locus_tag="PKI65_01650"
     CDS             15359..16711
                     /locus_tag="PKI65_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOB77178.1"
                     /translation="MNEIYKIKKGLDIPIDGEAPRMEIGFMLKEKIAILPPDFRLFTP
                     RLLVKEQENVIVGQPIIQNKLDENIIVTSPISGTIKEINRGEKRAIKQIVIEPDLNQK
                     YKEFDIPNSLDRDNIIDVLLVSGLWVYIRQRPYGIIANPIDIPKAIFISAFDTSPLAP
                     DVDFLLWGEDENFQKGIDVLKKLTSGPIHLNINPSKNRSDFFFKIKNVEHHFFEGPHP
                     AGNVSVHISRISPLNKGDIYWYLYPQDVMFIGRLFNTGRLDLHRKIALTGSELTERGY
                     ITILPGTAINSLIADRLKQDNVRVISGNVLTGTNAGIDGYLSYYDNQITVIPEGDYYD
                     FFGWVAPKFNKYSPSRMIFNWLVRKKDQKYVLDTNMHGEERPFVLSEQYDKYFPFDIY
                     PVYLLKAILSQNIELMEDLGIYEVVEEDFALPEYVCVSKINLQEIMRKGIDLMIKETT
                     "
     gene            16892..18082
                     /locus_tag="PKI65_01655"
     CDS             16892..18082
                     /locus_tag="PKI65_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOB77179.1"
                     /translation="MNWLRSFLDKIKPNFEKGGKLYFLHSTFEAFEVFFFVPNKVTEK
                     GSHIRDYVDLKRLMTHVLIALVPIVLFGMFNVGLQHYRLQLGIEIDAWGQVVKEAGFW
                     RIFWYGFLRVLPIIIVSYVCGLAVEFASAQIRHHQVNEGFLVTGMLIALIVPPTIPLW
                     MLAVATIFAVIFGKEVFGGTGMNFLNPALLTRAFLFFAYPASMTGDAVWIVNKGLDVI
                     SGPTPLALAAQNQFAQAPSLLTMTLGFIPGSIGETSKILIVLAAFYLIFTGVVNLRII
                     ISTLIGATAMGLIFNLWGANEYMQIPFYYHFFFGGFLFGTVFMATDPVTSAQTPIGKI
                     LYGLLIGILCMLIRVVNPAYPEGAMLAILLMNVFAPLIDHVVIQQNINRRKKRALLSQ
                     TIKS"
     gene            18087..18833
                     /gene="nqrC"
                     /locus_tag="PKI65_01660"
     CDS             18087..18833
                     /gene="nqrC"
                     /locus_tag="PKI65_01660"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOB77180.1"
                     /translation="MYKFTNKYIIIYSIIMVAIVAVLLTLGATALKPLQDKNKKNEKI
                     GYILRASRQEFDPKNAEDTYKKLLIKEIAINESGDIIAEFDYINGLRGKLRPFDIDVN
                     TETKKFKEGKSYILPIYLIQNNGDTLTVIPFYGMGLWGAIWGYVALENDLNTIHGAVF
                     DHKGETPGLGAEISDLPFQNQFIGKKIFDENQNFVGVAIVKGGVRRSNIPELHGVDAI
                     SGGTFTSKGLDNMLLDGFKMAMPYIEKHRN"
     gene            18918..19544
                     /locus_tag="PKI65_01665"
     CDS             18918..19544
                     /locus_tag="PKI65_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOB77181.1"
                     /translation="MKNENKDIDANHLPVMETFYSLQGEGYHTGLASFFIRLGGCDLS
                     CWFCDTKESWDPHLNELTSPQDLLDKAKKYKTKHVVLTGGEPILYPLQLLISLLHKHD
                     YIIHLETAATAPLINEIDWVCLSPKNHTNIHNEWFQKANELKVIITEAKDLKFAEECS
                     KLVSKNCHLFLQPEWHRSKILLPQIIEYILDNPIWRLSIQTHKFINIR"
     gene            complement(19950..20948)
                     /locus_tag="PKI65_01670"
     CDS             complement(19950..20948)
                     /locus_tag="PKI65_01670"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77182.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFKKFEGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEVVTSHRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNITYVN"
     gene            complement(21217..21711)
                     /locus_tag="PKI65_01675"
     CDS             complement(21217..21711)
                     /locus_tag="PKI65_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOB77183.1"
                     /translation="MNKLFLFFCLLISNFIFAQKPLPSINIFDINGNVFNTDSIKNEN
                     KPIIISFWATWCKPCLQELNAISDLYDEWEEMTGVKLYAISIDDQRTSATVSSFVKGK
                     NWPFIILKDPNHDLKRLLGVNNIPHNFILDKNRDIVWIHTGYSIGSEEEIIEKVKQLL
                     QNDF"
     gene            21831..22589
                     /gene="surE"
                     /locus_tag="PKI65_01680"
     CDS             21831..22589
                     /gene="surE"
                     /locus_tag="PKI65_01680"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOB77184.1"
                     /translation="MAKILITNDDGYRAAGLNALIEFIDPIADIVAISSETEMSGSGH
                     AVTIKNPLRLRVHEDHYKSIYVSNGTPADNVKLAVNCIFDKFPDLILSGINHGSNASC
                     NVFYSGTLAAVIEGAMLGIPSIGFSVDSYYKGYDFIIIKDYVTHIVKKALEFISLHKE
                     NILCLNINFPAIENPRGIKVCRMAKAKWVERFEKRIDPHGIPYYWLDGDFVFTDEGED
                     NDEAIVKQGYISLTPLSLDMTDYNIMDYLRSWEL"
     gene            22586..22876
                     /locus_tag="PKI65_01685"
     CDS             22586..22876
                     /locus_tag="PKI65_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77185.1"
                     /translation="MIRNFFKRDSWSLGIILGIVVPLIMFGLLAAIEALFSTGSADHI
                     FRLSTRIVISVFCNLLPFRHYMVKLKFDKTGRGILLDTFIFAIIFFIFFFSE"
     gene            complement(23030..23854)
                     /locus_tag="PKI65_01690"
     CDS             complement(23030..23854)
                     /locus_tag="PKI65_01690"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOB77186.1"
                     /translation="MEKTRVLFVSQEIYPFVKENELSKISRILPEKVSNDGKDVRVFM
                     PRYGCINERRYNLHEVIRLSGINVIIDNNDHLLIIKVARMPNTNIQVYFIDNQEFFQR
                     KFIFRDDKKNFFQDNSTRAIFFARSVAESIKKLGWSPNVIVLHGWMSALLAPMVKYVF
                     SKHPLFSESKVVLALHNDQFEEKLSDDTVDKLIFDDLTSDLFPNLLKDTNYINLMKDS
                     LMHSDAAIIMNENINSDILQFTKTLSIPVVDMSNEEDLLNNFNDFIDDLVAEELFS"
     gene            24060..24668
                     /locus_tag="PKI65_01695"
     CDS             24060..24668
                     /locus_tag="PKI65_01695"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77187.1"
                     /translation="MKAFVFSVKSLNAEDFEIIIELLSNQTFEDFFIALKNAIGFTDN
                     ELASFYLCNSAWKPKKEIALIDMGQDDDENNKVLIMSKTKLNIIEDPHQKILFVYDYI
                     KNHKFNIELIKIKDRDPDIEYPNIVKIKGKYIKTLTPNIPIPVTTDDDILDEEDDEEE
                     ILEEFDVVVDNISEDENDTDDIIDEHFFEDIADKTLDDFENE"
     gene            24726..25745
                     /locus_tag="PKI65_01700"
     CDS             24726..25745
                     /locus_tag="PKI65_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HOB77188.1"
                     /translation="MNTLEIIDKESKDKNILIIGDVMLDRYIWGDVARISPEAPVPVV
                     NVTKIEHRLGGSANVALNIQSLGSNPFLVGVIGNDASGNEFINLMKSQKMITEGLITA
                     SDRQTITKTRIMGNNVQMLRVDDENSKNLPDSLRQQVLLKITNLLEKHKIHAIIFQDY
                     DKGVIDPLLIKHIVQLSKTYDIPVCVDPKKRNFDFYNNVTLFKPNLKEFREGINWNDE
                     AFNMNTLFDKIHQFREAKNIKYMIVTMGEKGLVLAKTNEINNTFYTISIPPPKIRQVR
                     DVSGAGDTVISVVALGLALGLNIELIAELSNLAGGIVCEYVGVVPISKKQLLEEAAIY
                     LKNVK"
     gene            complement(25999..26073)
                     /locus_tag="PKI65_01705"
     tRNA            complement(25999..26073)
                     /locus_tag="PKI65_01705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26036..26038),aa:Val,seq:cac)
     gene            complement(26081..27091)
                     /locus_tag="PKI65_01710"
     CDS             complement(26081..27091)
                     /locus_tag="PKI65_01710"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOB77189.1"
                     /translation="MPRAVITGVAGALPDYILTNEELSRMVDTSDEWIMTRIGIKERR
                     ILKEPGKGTSELAKQAVQKLFEKTNTSPDEIEFLLCATITPDHQFPATANIIADKVGI
                     KNAFHFDMNAGCSSFLYALAIARAYIEAGHAKKIIVVGADKMSSITDYTDRTTCPIFG
                     DGAGAVLMEAYYGDDLGIIDMILQSDGVGRHHLYQKAGGSVWPATIETVKNREHYIYQ
                     EGQAVFKWAVSKMADVSVEIMKRNNLTNETIDWLVPHQANVRIIEAVAHRMKLPKDKV
                     MINIERYGNTTSATLPLCLMDYEHLLKKGDNIILATFGAGFTWGAIYMKWAYDGNKFI
                     QK"
     gene            complement(27119..27298)
                     /gene="rpmF"
                     /locus_tag="PKI65_01715"
     CDS             complement(27119..27298)
                     /gene="rpmF"
                     /locus_tag="PKI65_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOB77190.1"
                     /translation="MANPKRKFSKSRTRKRRSQYKIKTVKGVTCPNCGSPMIYHHVCP
                     ECGYYRGKLAIERNE"
     gene            complement(27323..27787)
                     /locus_tag="PKI65_01720"
     CDS             complement(27323..27787)
                     /locus_tag="PKI65_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOB77191.1"
                     /translation="MITKLDYKINYQALNLGHSYYDYDIDDTFIHDYNPNFDEMRDVF
                     IHIKIDLLKTETCAELFINLKGSYNVLCDRCLSFMKIDIDKNDYLIFKEGIGEDDETI
                     IYISQGTEFIDLRPHIYDFIALSMPMRHVHNDESDCDQEMLNKLKEYLIEDY"
     gene            28020..30284
                     /locus_tag="PKI65_01725"
     CDS             28020..30284
                     /locus_tag="PKI65_01725"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOB77192.1"
                     /translation="MCIYKIFFKSKNENRKNSNREQNSEISTNDIINDLDINDDKDIE
                     TLLMKLSGRVKETNTNLINRGLKEILSDYSFDDLKLDDKSKKKILELKELAKNIENKI
                     IKEPNYDATKNYKIDYANELNNEQLIAVTTTDIPLLVIAGAGSGKTRVITYKVCYLIE
                     NGVNPNSILLLTFTKKAAQEMLDRVNKLLKNKIGGNVLGGTFHSFANYILRRYSKVIG
                     IKDNFTIIDTTDSADIFSLLKSELKINKKIDGKPFPDKKELQEITTKARNIETSINNV
                     IDKFFYKYIDYTDEIITLIKYYEKYKKTFNLLDYDDLIIILRDNLRDNAGFLKAVQNE
                     IEYVLVDEYQDTNTIQRDIADLIASKNEKITVVGDDAQSIYTFRGADYENILRFPQFH
                     PNCGVVKLECNYRSNQNILYFINSIISNAQIGYKKNLYTNMQSSQKPIFKKFANERDE
                     AKFIVDKILEIKGDILNYNDFAILTRAAWHSNVIQTELMARGIPFDVIGGIKFAERAH
                     VKDIIAFLRIIINHIDAISWHRILQLIDGIGNVRATEIITSINKKNGIIEFSEFQNRK
                     YYDELNRLENMYNKIINNDNIKIDEIFNLIYDFYIPLFKKNYGNYDEREQDLEVLRII
                     TSNYESIEKFLDDFTLEPPNNKFHNGVFPGSKNNKDTVKVSTIHSAKGLEWNTVFIPH
                     AIDGLFPSEKSLSTLEDLEEERRVFYVACSRAKENLFITIPVYLESYRGYTNKPSRFI
                     YEIDPKFYFIEGND"
     gene            complement(30784..31749)
                     /locus_tag="PKI65_01730"
     CDS             complement(30784..31749)
                     /locus_tag="PKI65_01730"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOB77193.1"
                     /translation="MVDLVLILKGIAIGTANVIPGVSGGTIALITNIYERLINALKNF
                     NFTAIKLLFKGKIKELIEYIDFYFLLQVFTGVLIALFSVAFLLKYLFLHYPIYTWAYF
                     FGLIIGSAWIIIKGLKSLRWWDWLLLLIGISIPILMVFMSPINENDNLFYILLCGIIG
                     TSGMILPGISGSYLLLILGNYKLIMLDAVTTLNLKILIPFAIGSILGLILISYVLSIL
                     LKKFYNETLSVLAGFVIGSLALIWPWKYSIDHTHGLILQNKYGALINANGDLINFKPY
                     IVQYQYYLPNIDNQFFVALLLMLAGIITVVGTEIISDKVSKKRKK"
     gene            complement(31824..32621)
                     /locus_tag="PKI65_01735"
     CDS             complement(31824..32621)
                     /locus_tag="PKI65_01735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOB77194.1"
                     /translation="MQKRHSDRIAYFKMQAEVCEKVIIPLLSPFIDINKKQKLLEIGC
                     GEGGNLLPFVKRQWTCTGIDISSSRITDGKKIYETFNLNNAPHLIVGDVCDLQPNDTY
                     DVIIARDVLEHLPCQNNFLKNIDRFLSDNGMIFINFPPWFSPFGGHQQICKNKILSHL
                     PYIHLLPYHTYKKLLQLFGENEIIINDLLEIYDTRITTEKFKHTLKDSNLNIIWERYY
                     IINPAFKIKFNMKSLKLFFPFNKIPFFRDFITTGYYVILKKKNQLAM"
     gene            32748..33323
                     /locus_tag="PKI65_01740"
     CDS             32748..33323
                     /locus_tag="PKI65_01740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOB77195.1"
                     /translation="MNLFSKIRDAILRRISKNIHPTMILGYKQNSEYLDDVRISNTTV
                     INARENLKIANHVFIGHYNFIEASNGIIIEEGCQISNFVSITTHSSHISIRLYGADYT
                     KYKNLYGYVTGPIHIGKYTFIGPHSVIMPNTTIGKGSIVTAFAYVEGEFPDFSIISGN
                     PAKIIGDTRQIDKIFLENYPELQILYDQWAR"
     gene            complement(33371..34288)
                     /locus_tag="PKI65_01745"
     CDS             complement(33371..34288)
                     /locus_tag="PKI65_01745"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOB77196.1"
                     /translation="MTTSILDYIGNTPLIEIKKLNPYFPEIRIFAKIEGQNPGGSIKD
                     RPALYMINDAEKKGILTKEKTIIEATSGNTGIALAMIAAIKGYKIKLFMPECVSLERR
                     KILESYNAKLVLTPAKENTDGAIIRAKKLLNDYSDLYFMPDQFSNPANPQSHYETTGP
                     EILNQMPCQINYFVCGVGSTGTLMGTGKFLKENIQNIKIVAIEPVKNHKIQGLKNLEE
                     AIVPSIYDSSLFDMHFYVNDNESFELARKITREEGVFAGMSSGAALAGTLKLAEKIKS
                     GNIVTIFPDRGDRYLSTNLFASFCANCPP"
     gene            complement(34305..34997)
                     /locus_tag="PKI65_01750"
     CDS             complement(34305..34997)
                     /locus_tag="PKI65_01750"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HOB77197.1"
                     /translation="MKTKTFIILLLISLSTFFVACEDENDNNGNTTNGDTTPPIIYLN
                     GASDTSIALNTQWMDPGATAIDDVDGVVPVTVTGNVNKDLVGNYTLTYKASDSNGNTS
                     IKTRIVRVKNEADTWAGTYTVTDNASGNTYTYTDNISISQTKNKYANVNKFRNYTNCT
                     LELYFTEPITVNTSIIINSQSITCGDPYISRTFFGTGQITSITEHTFELFFNENDGTN
                     TITGVENYQKNN"
     gene            complement(35027..37657)
                     /gene="alaS"
                     /locus_tag="PKI65_01755"
     CDS             complement(35027..37657)
                     /gene="alaS"
                     /locus_tag="PKI65_01755"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOB77198.1"
                     /translation="MTSYEIRKNFLDFFRKKDHIIVPSAPIVIKNDPTLMFTNAGMNQ
                     FKDIFLDLQEPPYKRVANTQKCLRVSGKHNDLEQVGLDHYHHTMFEMLGNWSFGDYFK
                     NEAIHWAMELLTDIYKIDIDQLYATYFEGDTAENLDEDTEAKKVWLSVLPADHVLPGN
                     KKDNFWEMGNIGPCGPCSEIHIDLRSKEERKESVGANLVNKNHPEVIELWNLVFIQYN
                     RNADGSLSLLPKKHVDTGMGLERLTRVLQNVSSNYDTDLFQPLIKKIEQLSGIPYKAD
                     PQKDIAFRVIADHTRAISCAIADGQLPSNVKAGYVIRRILRRAIRYGYQYLNISEPFI
                     HKISQTFIEHYCEIFPELKQQSELIEKVIKHEEITFLSTLERGIKLFEQYIENNKNAK
                     IIDGQFAFELYDTYGFPIDLTQLMAKEKGLSVDIKKFEEFLNIQRERSRQSAQVDTAD
                     WIIINEGESEFVGYDTLECPVKIIKYRKTVQKNKTFYHLVLDKTPFYAESGGQVGDSG
                     IIYSDKETIFIINTIKEYGEIIHIAEKLPQDLQSIFIAKVDVEKRFSTMRNHSATHLL
                     HHALRQIIGPHAEQKGSLVHPDYLRFDFSHYEKLSRQQLNDIEKLVNKLIQDNYKLEE
                     HRNISYQQAIDMGAIALFGEKYDERVRMIKFGDSKELCGGTHVKATGDIGCFKIINET
                     AIASGIRRIEAVTGMKAIEIFQETYDILSDIKSILKTNRPLESVENLIEKNKELTKQN
                     DELIKEKLNNIARNLIERAEKIDNLHFIGEIIDITADQMRPLSFILRTFNKNIIAVLA
                     SSKSEKANVAFVLADEVIKKYNFTTKDLINIASPIINGKGGGQPNIAIMGGENKDNYI
                     ILIDEIKKFVLKKIKSINSQ"
     gene            37803..38945
                     /locus_tag="PKI65_01760"
     CDS             37803..38945
                     /locus_tag="PKI65_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOB77199.1"
                     /translation="MKNEMELNLFEFNENDDLKKNVSVYFNKEPLIKVLDDFEQMNDI
                     NKIKKEFLYLIQIIENPKEDRVGLISINNKGDIIECNKYYIITEINTILKSQTIERIH
                     YYIKRLKKSLLEVKTNKINDLNLNQWKKYDNILTDSLWILDKRDSSGAHNAGYWGNFI
                     PQIPNQFLQRYTKKNEWVLDPFLGSGTTLIECKRLGRNGVGVELLPEVVELAEHNISL
                     ETNIYNVKTEILNADSTKLNFKDELKKLNISSVQFLIMHPPYWDIIKFSENPNDLSNA
                     NTIDSFLDLLGKVVDNTYDILDNERYMALVIGDKYSKGEWIPLGFYCMQEVLKRGYLL
                     KSIIVKNFEETKGKMNQKELWRYRALAGGFYVFKHEYIFLFQKSKK"
     gene            38942..40093
                     /locus_tag="PKI65_01765"
     CDS             38942..40093
                     /locus_tag="PKI65_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77200.1"
                     /translation="MTTHVFIVDINTFKYHLEYLFAGTGAQDLYIDFNNNSNSSLNYA
                     IENNLVSMISDSQRIRKGDYIIFYLQQNKAKKVYEGKFYGIFKAKDNHSFLDNNDKNQ
                     FLKDNLNKSLTFRTIIEPYKVYPIGVTEWEALDEIKYIQSPNQMLWSLIYRKLRANRG
                     NTMITIYESERLIKLIKDKNNGKTLNCNNFTFDTNTQEIIGNNAKYPYTGRKENINIL
                     PRLINKFNQGKSFEPHLQAYIVQNIGRKTHNNLDNLLVNNYDVEWIGNEVYCGVGMQK
                     IDIMLSLIKDDERIIEPIELKSVCATPDIIFQIQRYIDWIEQYYIPNRISDIQPVIIS
                     KKISNKQSSNYSLLINNFKNLNDKNIILPLKYIEFEIINNNIIFKEILY"
     gene            40152..40225
                     /locus_tag="PKI65_01770"
     tRNA            40152..40225
                     /locus_tag="PKI65_01770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40186..40188,aa:Arg,seq:acg)
     gene            40481..41680
                     /locus_tag="PKI65_01775"
     CDS             40481..41680
                     /locus_tag="PKI65_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOB77201.1"
                     /translation="MGRAMIIGAGGVATAAACKMTEAPEVFEEVMIASRTKEKCDKII
                     KQIDKTNYYSAQIDAMNINELAALIKDFKPDIVLNLALPYQNLAIMEACLQTGVSYLD
                     TANYESPDNPHFDYIKQWSYFDKYKSAGITGILGCGFDPGVTQVFTAYAAKHYFDEIH
                     YLDIVDCNAGDHGKYFATNFNPEINIREVTANGRYYEDGKWIETKPLEIHRPIEYPEI
                     GVMESYLLFHEELESLVKNFPTIKRARFWMTFSERYLTALEVLQEVGMTSIKPINYEG
                     KEIVPLQFLKAVLPDPSTLGENYKGKACIGVQLKGIYQGKERTYFIWSIFDHEKAYKE
                     TGTQAVAYSTGVPAMVGTMLYLKEIWKKPGVFNVEEFDPDPFLETMPIYGIDWYEAVD
                     IVLPIDK"
     gene            41683..42822
                     /gene="nspC"
                     /locus_tag="PKI65_01780"
     CDS             41683..42822
                     /gene="nspC"
                     /locus_tag="PKI65_01780"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOB77202.1"
                     /translation="MSRKYFTEIPSPCFVHEENKLLNNLKILDYVRKKAQVQILVALK
                     GYAFWYYFPLIKKYLDGASASSLFEAKLINEEMGVKAHTYCPAYLDEEFDEIAKLSKH
                     LTFNSIAQWEKYNKRIPTGTSCGLRINPEYSEIEVALYDPAIRGSRLGIVRAMMPENL
                     PLGIEGLLFHVMCEQDSYVLENVLEQVQTKFHHYLLQAKWLNMGGGHWITDDNYNVEH
                     LIALLNNFHIRYPHLIIILEPGEAIGLDSGFLLTTVQDIVENNGIKTAILDVSFSAHM
                     PDTLEMPYKPIVRDASIDPANKPYSYRLGGITCLAGDFMDEYGFDEPLKCGDQLIIDD
                     MINYTMVKTTLFNGVKHPHIGILDIDNKFKLLRSIPYEHYKYRLS"
     gene            complement(43024..45624)
                     /locus_tag="PKI65_01785"
     CDS             complement(43024..45624)
                     /locus_tag="PKI65_01785"
                     /inference="COORDINATES: protein motif:HMM:NF009397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOB77203.1"
                     /translation="MNMETEKEKNIEQSKGKTSLKTISGMYENWYLDYASYVILERAI
                     PHIRDGLKPVQRRILHSMNELEDGRYNKVANIIGNTMKYHPHGDASIGDALVQLGQKE
                     LLIDTQGNWGNILTGDTAAAPRYIEARLSKLALQTIFNHKITPWKISYDGRNKEPIYL
                     PVKFPLLLIQGIEGIAVSLQVRILPHNFNEVLDACISYLKDESFTLYPDFLSGGLIDV
                     TNYNDGQKKGQVISRARIEIVDNKTIVIREIPYGVTTGQLIDSIIDATNKGHLKIKKI
                     EDNTASEVEITLILSPDVTPDQTIDALYAFTKCEVKISTNCVVIDDNTPVFLSITELL
                     KETTDNIVGFIKEELEVEKHELLEKLLFASLEKIFIENHIYQNIEECETWEDVINTID
                     KGLEPFKKQFYRDITRDDIIKLTEIKIKRISKYDAFKADEIIVKLQNQLEQVNINLNN
                     LTSYTIEHFKYLKNTYGKNFPRRTKIRTFSSIEKDKVAAPNQRLYVNRTEGFVGTSLK
                     KDEYLFDCSTLSNLIVFKEDGTFRVIKVDDKVFVGENILHVAIYDPNDTRTTYNVLYR
                     DGANGNVYAKRFFVKSVHRDKDYFYTRGNPNSKVLYFKANQNGEAEVLTIHLVPRPRM
                     KNLTIDFNFADIPIKNRSTVGYVVTKKLIRHISIKQEGIKTLEGISYYFDNETLRINK
                     DGKGVKLGEFFDDDYLIIIYKNGYYRIAIPNESLFIDSNYLLVEKFDPRTLYTVIYRN
                     NDQKSQHIKRFILDEIIDKNVYFLPENDDCEILFFTKEWLGNLQINYSNKTKEIIDLD
                     KKFPINSLKAKGKKIKNNKIKNIEFINVIKPDEAWLKSIFPENIIDNNIIDDSPDIEL
                     DTGQIILSLK"
     gene            45917..46378
                     /locus_tag="PKI65_01790"
     CDS             45917..46378
                     /locus_tag="PKI65_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77204.1"
                     /translation="MKYLFLCQGNVCRSNMAEEILKERLMQINSDSLVDSAGFEPYLI
                     GEETDKRAQNALLTKGYKPSTHRARLFQKTDFDDFDKIYVMDANIYRLVKEMARNNKD
                     IEKIDYLMNEAYPEKNIPIVDPSFSDNLNDFINTIEMIENAIDAIIEKYEK"
     gene            46507..>47019
                     /locus_tag="PKI65_01795"
     CDS             46507..>47019
                     /locus_tag="PKI65_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77205.1"
                     /translation="MKKIVLLIILIISTSIIFGQNNQNDERIVPIEQKFQEANWVTTI
                     TDLDFSNIILLSSFDWQAIKHEDSIRYNQKLPTRIGMSIAIDVDLLNDIIPIEEDGYY
                     IYRYKVFAEGAKKVGFVFDDFFLQPGDAIYIINEDGQFIGPVTDVENKEFRMYSTVPL
                     NGEEITLLYVK"
BASE COUNT        16264 a         6615 c         6749 g        17391 t
ORIGIN      
        1 tcatcagaca gacgagcgtg gtatgtatat agggacttga gagcaagtcc tgtgtttatt
       61 taaacattga agaatattat tatctctcac cttatcaact ataaaattct atttttaatt
      121 tttaaaacta ttaagctcgt ataaaacagc atattaagca atacatactt gaattagttc
      181 aatttttttt aataaaactt ttgttgaatt ttatattatt atgagcagta caattaatta
      241 atcgtattaa ctttatttac aactaattat aaaattttat taaatgaaaa aataaataat
      301 ataggaataa tattataact ttttttagaa tttattaaaa aagttaaaag gatacactaa
      361 aatttttaga ttaaataata tgcgagattt agtgtatcct ttttattttg gtaattaact
      421 atctttactt tgttagtctt attttaaaaa gatattaatg taacaccaaa ttctaatggc
      481 ataacttcag ggcctttatt ttctttaaat aacggtgaaa aattatattg aactgtaaat
      541 gataaatgtt taatgccaaa tctacaaact accccatatt taaatggata taagctattt
      601 attccatttt ctttttctac cttatctggt gaagagtaaa caattttagt atgactacct
      661 atcaatacgc ctccgatagc tcctacgctt atatgaaaat atggtttagt ttttgcttga
      721 tattcaaata ataatggtaa ttgtaaataa cttgcggtca gtttagattt tttgacgttt
      781 ttagcagtat ctattaaaaa gcttatccca ttcttagtat cagtgataat aggtttttgt
      841 ccaaatctat aatttaccca ttgaataccc aagcctgtaa ccatacctaa ggtaccattt
      901 ttattaaaag gaatggactg ttgaaaaaaa ttgaattgca ctcccgaaga tttaggaaaa
      961 tatatatcta aaaaatcata attaggcggt aatgaatatg ataaggatgg atctgcataa
     1021 ttagtaaaca aaaaatctaa cgatgcccaa tgtccactaa attctacctt tttgttagat
     1081 tgttttgttt tcttatcaga tttgtcttgt atttgatttt ttgtatcact atcctcttca
     1141 ttattttcat catcaaatat gaaatctgaa aatgggaatt caaatgatat ttttttatca
     1201 aatttatcta aggtatcata ttgtttgata tatttatttt ttataaaact atcatcttca
     1261 atattaacat atttaggagt gccatagtat gtaacaaatg atgcattatt agatattatt
     1321 tctaaagtat cagtagcttt tactttcact ttactaactt catttgcttc tacgtatact
     1381 gttttagcat ttatatctgt agcatttatg taactcactt cgctagcttt gatattaaga
     1441 tagttacatt cacctttaac atttatataa cttgcctctt cagctacaag ttctaaagta
     1501 tcacataaaa cattgatttc gcacatagaa gcttcatttg cagtgatttt caaataattc
     1561 gtttgaaatg tatcattaca agtcaatctg cttgccaatg ttaaatttaa ttctttaatg
     1621 tttggcaaat agatctccat tttgtttaca gtttctatat ctaatatttt attaatagaa
     1681 tcgtatagaa tagctatgtt attgtctgca ttagcttcat aaattaattt tccttgtcct
     1741 tgctttagaa tatacaaacc acctgttaaa tttaattttt caatttctcc aagatttttt
     1801 tctacttttt gcgcatttac agatgtaata attaatatta aaactgttac tagggttaaa
     1861 actttcgttt tcataattta tcctttttta atagtaaaac gatttaagat tataaaaagt
     1921 tacaaaattc aaaaaaaaaa ataataataa gtattaatgc aattcattac ttacagaagt
     1981 tgatttagaa ttttaattat ttacattcta ttacagatag tgtggttaat cgaattttag
     2041 tttagcacat aaaaaatata taatattaat aaaattgttt atatttttat tgccttattg
     2101 ctcttctttt agcgagttga attttattat tatcattatt tcataaatgt ggaacacaac
     2161 tattagtatt ataaatattt tttctctaaa aataaattaa cataaattga agataaaaaa
     2221 tgacaatata tagataaacc aaccttaaat agtattaaat ttagttcttg aacttttcag
     2281 agtagactca tatattaagt tagttgattg tttaaagtgt catgtcaata gtacattata
     2341 tagggataat aaaatatatt tatgaaaatg agtttgagaa agataataaa actaatatat
     2401 gtttcaatca acatatgtta tattcactaa ctctctatac cgataccacg cccacctgtc
     2461 cgaagatgcg aggttgagca ttcgcccagt attgcttata gtcttgcctg tgaggtattt
     2521 acccatcact acataccaca taatgtagtt ttgcaggtat ttggtagcta cgcccctaaa
     2581 tttgaaatat atccaattcg tgaattcact agcctttttc ttgacggtac ttaggctata
     2641 tatacctcgc ttttgcctat tgctagttac tatttctttg tgcttgatta catttcttct
     2701 aacctttttg aatgcttttt tattactact acacatgact ccatcctttg gcatcttagc
     2761 cttcagaaat tgctctatct gttctgaact tagctttttg ccatcaaatt ttttgaataa
     2821 taagttgcca gctctgtctg tagcagccaa tacggctact ttctttggct tttgcttcct
     2881 agctcgcttc agctcttcgg catttttgta tattctgcct ttttcggaat aatccatcaa
     2941 tagttcatcc atctctacta caccgaaaaa attgatatta tcttcgaaac tttgtaaagc
     3001 tgccaatatt ttgtgtcgcc aagcgaagct tgttggtagg ctgatgccta cttctttagc
     3061 acaagctctg agacttttgc ctgctaccat acatctgatg taatcaagca ttttctcttt
     3121 cttttgtaac ctgtaaacaa aagtacctgt ggtaactcta aattgtctgc cacagtcttt
     3181 gcagcaatag ttttgcatcc cattttgtaa accattttta atgatgtgat tgcttcgaca
     3241 gtctgggcat agtggtgggg cactgagtat tttcagacct tgataatcga ctacatcggc
     3301 ttctaatttt ttcattagag aataaaggtt gtctaagaat tctttattct cttccttact
     3361 catagcaagg tatttgctgg tattctctac gatttttttt atagcctaaa atttttgcaa
     3421 aaatataaaa attatattta atacctactt tgttaattta aacatataaa aaaaatcctc
     3481 tattcctcat ctttaccaca caattcaata tattatgaag caaaatcatg aaaacattta
     3541 tcataaatct tttttaattc ttgtatatta aatttatgaa tttttggctt tccaggtgca
     3601 tataagagta taaatgttat tttatcatca ctattttttt tgtcttgtaa taaataaggt
     3661 agaagatcat taaatttgat ttttggtggt tttccatacc aagtttgcaa ccattcaatt
     3721 atttttttag accaatatgg tgggaaattt aataaatatt ctgaaatttt aattgtatat
     3781 tgtagccccc atgctatgca tattccatga gaagtaggta aatgattaga aaatcttata
     3841 gattcaaaac aatgcccaag tgtatgtcct aaattcaaaa tttttcttct atttttttca
     3901 tttggatcta ttcttactat atgatgttta aattgaatcg acttttttaa tattgaaatg
     3961 ggaggtagct tacgtggatc taaatttaaa atttctctta ccgaatcttt attatttaat
     4021 aatgcgtgtt ttattatttc tgtgaagcca cttaatattt caatttctgg taaagaattt
     4081 aaaaaattag gatagatata agtaaataat ggagaataat atgttcctat ttcatttttt
     4141 atatttttat aattaattgc atttttgcct cctatgctag cgtctgtcat tgccaataat
     4201 gtagtgggga ttatcactaa attaattccc ctcttaaatg tagcagatgc gaaaccagct
     4261 atatcgctaa tagtacctcc acctatactt attaataaat cttttttatt aaatttatca
     4321 ttacttaatg tatcccatat attttttata ttatctatat tttttacatt ttcatctaca
     4381 ttatatttca ataatctaac gtgattaaaa tatggataat taattaaata gtttaattca
     4441 ttttcataaa tagatgctac taattcatca gctagtatta agcatttgta tttttgatgt
     4501 gtacctatat gatatcttag gaatttaaaa atattcaata aattttcttc tacatatata
     4561 ggatttttat tacctattcg cataaattta ttattaatat aaattacata attatttttt
     4621 tgtttaaatt attcatcgtt tatactcttt gttttattat accccatctt aaaatacctt
     4681 ctaatagtaa gcaaatcaga gctataaaca atggaatgtc aaaaatatct tgatgtgata
     4741 caatggtact ataatgaaaa cgagtttttt ctaattgatc tatctcttca taaatttgct
     4801 taagtgattc attatcagta gccctgaaat atttaccatc agtattattt gctatattta
     4861 ttagcaaatc ttcatctatt aatacctcta cataatcata aacaatacgt ccaaaaggat
     4921 cttgaaaagg atatggtgct ttgcctcgag tgcctacccc tatggtatag atatgtattc
     4981 ccatagtttg tgctatttgt gctgctgtaa caggatctac atatccagcg ttatttacgc
     5041 cgtcagttaa taatatcatt actttgctat tagcatcact tttttgaagc caagatattg
     5101 ctaccattat tccatctccg atagctgtac catcagttat aatgccagtt cgcaattgct
     5161 tcaattgata tttcaaaatt tcatgatcat aagtaatagg gcaagctgta tatgcggatc
     5221 cactaaatgc tactaatcct atcctatcat taggtctttt atttataaag tcttctgcaa
     5281 tattttttgc tgcttctaat ctattaggaa tgaaatcttg tgctagcatg ctgccagaga
     5341 tatcattgac caatactata tctattcctt catgttctac tgatgaggag acatttttta
     5401 attgtggtct gctcatagct attattataa atgctaaagc taataatctg agataaggta
     5461 gtgaattgaa taaaaaggtt ctaatacctt tagttttcaa tggtaatgcg tgtatgtgtg
     5521 gccatttaat aaaattatat ttatgatacg aacgtttctt tacattccac caccacaaaa
     5581 tcggaatgag tattaatagt aataaaacat aaggatattc gaagactaaa ttattcatat
     5641 tgctctttct ttaattgctc ttgctcccat aaataattga taaattgatt acaaaattca
     5701 taatctctat atctttcttc tagggtagga gtgtatttgg caaattttgt aagatctgag
     5761 gtgtttaaaa attgttgaag cttagctaac cattcagtag agttacaata tttatgcatt
     5821 tctaaaaata tttctgatga aagcatttca aaggttttta tattatatat tctcaatata
     5881 aatcttctta aggtatctgt caggatagta taatattctt ttgcattcat ttgagcgata
     5941 ctattatttt cctttaactg tttcagagct tgcatagctt cctctatagg ggttagctca
     6001 ggattgtaat acaaactttt ttgttctttt ctgattttat tgtttctata tattaaaatt
     6061 attatcaata ttagtataat agcacttata ctgattatta gccatagcca ccatttactt
     6121 ttcaatggag gttgagaaat atcatatata tttttaatcg gtttataagc ctttgatgta
     6181 tctatcggca taggtactac attaaatcct atactatcta ttattaaatt tgttaatttt
     6241 tgtttgtttt tataccaaag ttttatattt aattgtggta gaaaaaaatt accagagtca
     6301 aaggctgtta ttgaatagcg tttttcccaa gtgtgattat ttgtttttat ccagggatga
     6361 tctatagata tatataattg ttttggagtt tttttaataa attttgaact aatagaatct
     6421 atttgtgtat tttgtgaatt tattattatt ttaatatttg ctaattcacc tatatatatg
     6481 gtatcttttg aaatctcaac tctttgaaat atatcaatgt cttgagcatt taataaagta
     6541 gttaaagata aaattattat tataaaagta tacattttat aagaaaagtt agatctaaac
     6601 ataatttttt tatttacatt ttttcaaaaa aacactagtt gctactgtat atactcaaac
     6661 tcatctgttt tatgttgtaa gttgtttttt tttttgatca tcattattct atagttttaa
     6721 attataaacg tcaaaactaa actaatcttt tttctctttt tttgaacaaa gcagaaagca
     6781 aagggatgta atctatttca gtgtcaatag atagcaagtc tatattgtgt tttttgcaag
     6841 ttttttccaa atagttataa tgcacttgat aaacatcttt ccaggcagtt acagttttgg
     6901 gattgttaaa atctagaggt acaaattgtc cggtttcagc atcacaggta atgacaaaac
     6961 cttcggtagg gggtgttatt tcgagtctat ccactatctg catagctatg aaatcatgct
     7021 ttttattagt aatacccata gctaattcat attgagtatc ataaaaatct gatattaaaa
     7081 aaactatact tcttttaggt attacatgat ttagatattc taaggctttc ttaatattag
     7141 tttttggatt ttcgggttca tatgctagga tttctcttat tatagtgagg atatgagata
     7201 atcctttttt aggggggata aatttttcta taacttcaga aaataatatt acaccgatgc
     7261 ggtcattatt tttcaatgca gagaatgcta gtataccggc tatttcagat ataagttcat
     7321 ttttcatttt accagtactg ccaaaaatag tagagcggct aacgtctatc attaatatca
     7381 atgtaagttc tctttcctct tgataaactt ttatgtatgg atgattatgg cgagctgtta
     7441 cattccattc gatatctcta atatcgtctc cataaacgta ttctctcaga tcagcatagg
     7501 ccatgccttt acctttgaag gcactttgat attgaccagc aaacatttga ttagagagtt
     7561 tgcgggcggt gatagagata tatcttactt tttttcttag atcttctaca cttagactca
     7621 tggtacttct attacattta atatatcagc tatgaattga tctgttgtaa tattttcggc
     7681 ttcagcttca taagtcatac caatgcgatg gcgcaataca tcgaaagcca cagctctcac
     7741 atcttcgggt atcacataac cacgatgatg aataaatgca taagctttag ctgctaaaga
     7801 catataaatg ccacctcgag gggaggcacc gtaacgaacc atatttttat attgtggcaa
     7861 tcgataagct tcgggttgta tagttgcata gttaatatct attatgtaat tacgtatttt
     7921 ttcatccata taaatttcta gtactaattt ttgtgcattt ttaatgtctt tgggagtggc
     7981 cacagcattt atggttggaa atttttcttg taaattgagc tctatgatat atttgagttc
     8041 atttttttgt gggtaatcta ctaaaacttt catcataaag cgatctactt gtgcttcggg
     8101 tagtggataa gtgccctcct gttctaaagg attttcggtt gccatcacta taaatggatc
     8161 tggtaatgga taagtattat tgcctatggt tacttgcctt tcttgcatag cttctaataa
     8221 agcactttgt actttagctg gagctctatt tatctcatca gctaaaataa aatttgcaaa
     8281 gatggggcct ttgcgtactt gaaattcttc tgagcgtgga ctaaatatca tagtgcctat
     8341 caaatcggca ggtaatagat cgggcgtaaa ttgaatacga ctaaaatctc cctgtataca
     8401 agaggctaat gatttaatgc taagtgtttt tgctaatcca ggtactcctt ctaataaaat
     8461 atgtccctta gtgagtaaag ccatgattaa gcaatttgtc atgtgctttt gcccaattat
     8521 tactttgctt agctccattt gtatcaaatc tatgaatctg ctcgcttctt ctatcttttg
     8581 gtttaaagat gctatgtcaa tagtattatc cataattaat tttattaata agttataaat
     8641 taatttacgt attttttaca aatttagtat ttattagaaa aatgaaaact atttttaatt
     8701 tacaaagctg taatatattg tttgaatttt aataattttt ttttaaaaag ccttaaattc
     8761 tgcaaactat atcagtcatt aactaccatt cacagatctt gtcaaagatt agtgataact
     8821 aagggggtta ttaattatga aaaaaccagg acaatttatt ttttataaaa tatcaatttt
     8881 tttctgactc attaacagct tgcctaagat atttagcttt ttcttccaaa attttaagat
     8941 cagttttagc attatctatt ttctttttgt attcttgtaa taaaagttcg gcattagctg
     9001 atgatttaaa aaaagaaaga ttattttccc aagtttgaat atctgagcgt aattttttga
     9061 ttttattatt tagattatat aattcacttt tagcttcttt taatccacca ctatgctcta
     9121 atattttttc aatacgttgt ttgaattcat cattactttc atctattcta acacctttta
     9181 actgatcaaa aagggaattt attttttgtc taaaagtatc ttgtattctt tttctttctt
     9241 tataaggtac attgcctatt tcgtaccatt gacgttgtat tgcttttaga cgttcgagat
     9301 cttcttttgg attattatta aattgaaaat catctaattc tttaagaata gcctcttttt
     9361 tatataaatt ttctacttct gcagttgcga gattagcata gtacatttct ttattagcaa
     9421 aaaattgatc acaagcagat ctaaatctat gccataaata ttcatttttc tcagctggta
     9481 tgaaaccaat ttttttccat tcttcttgta gttttagaat agtttgagta tcttttttcc
     9541 aattattgct atttacgagt ttttcagttt gttttataat acttaatttt ttgttataat
     9601 tttcttgcca ttcaattttt agaatttcat aatattcttt tttattttgg taaaatttgt
     9661 ccattagaga tttaaattcc acccatatac tttcattgta ttctttaggt acaggtccta
     9721 tggaacgcca tacattaaaa agatcttgta cctcattagt ttttttctgc caatcatcgt
     9781 gagttactat ttcttcggat aagatatatt tcattttgtc aataatagct tttttggctt
     9841 ctagattatt ttctcttcgt tgtttttgta gctctattga attgcgataa ttgtctttta
     9901 ttttatcaag ggttgtttga aatctttccc atatttcgtc ttttttatct ttaggtacag
     9961 gaccgatctg tttccattga tcaaaaagtt catatatttg tctatatgca tcttttggag
    10021 agatagtttc atccatcaaa agtgactcag ctttttcgca aagttctatt ttagcttcta
    10081 gattttttcg ctggtctatc tctatcattt ctttcaaaag ttctactttt ttgtagaata
    10141 gatctacata atgattgaag cgcatccata aatcattagc tttattatgt ggcactgcac
    10201 ctatagcttt ccactcttcc tgtatttgat taaaatgatc aaacgcctct tttagaggaa
    10261 tttcgttatt atctattata ttttttaatt gctcaagaat agctaatttt ttttcataat
    10321 tttcttctaa agattttata tattgatctt ttaaagattt tttcttacct aaatattgtt
    10381 tccatagctg atcgaaagtt tctttttttg ttttgagatt ttcttctatt ttttgtctat
    10441 ctttttctac agggatatta gataaatttt ttaattcgtt ttcataaatt aattgatgta
    10501 aatcatacaa acgaagtaca cgaggcctta tgtatgataa atcattattt tctaaaacta
    10561 tagcaaattc ttcatttatt tgatctaaat ctaaatgatc gtatttctct ttttcctctt
    10621 catttacttc tatatcatca tcttcctctt cgtttacttc gtctatttga tattttaatt
    10681 ctatatcatt attgacatct gtgggtaaaa attcgtcaat tgcattattg ttttcactca
    10741 tattcagtga gagttcattt ttcttttctt ccttccccat gatattcgat tttttataaa
    10801 gtttatcata atttatctag gttgcaaaat taagataatt tttttaatta attaaattat
    10861 atttgataat tatgtttttt ttatgtaata aaaaacttct taaatattat aaattttaat
    10921 ttataactgt taattggtag taaattacgg atgaataaaa aagcccttca tgcaaatttg
    10981 taaataaatg ttattatgta aaaaaattta acattgctaa taaaaatcag tttctcaaga
    11041 atgacaaaaa acaataagaa gtagtgttgt agtagttttt aggagtttta gaagctaaat
    11101 aagttgtaaa tcattaatta ttttcaatcg attttaagtt gttatttatc acctgatatt
    11161 gttctctgct ggttttttga tcaaacaaaa aatattttga tattccgcta gctttcacta
    11221 tatcattttg tattatttca gcttggataa aagcaatatt ttttttaaca tctactaatt
    11281 taccacgtag tgttagtaat tgattagatg agatttgttt gtgatatctc acatttagtt
    11341 ccacagtcat accggctatt ccgattttgg atactactac ccagccaccg atttcatcta
    11401 atagagtagc tattataccg ccatgtaata caccttgcca cccttcataa tttttattag
    11461 gcagccattg agtttcaata taatccccca tatcataaaa atctaatttt aatccaatgg
    11521 gatttttttc tgagcaacca aaacaaaaat catctttttt agtccataat tctttttcta
    11581 tttttttcat ttcactcgtc tttttgcata ttcatctaaa aattcttttt ctgtatttgt
    11641 taatgaagtt tccccttcat tttttatttt ttctaaaatt ctatttattt cattttcgaa
    11701 ttctttttgc ctatcataat aatcataatc attgatagga ggtatatcat tcaatattgc
    11761 aggattatta gatatatatc tgggattttt atatgaaata cagcctagta tagctcctga
    11821 gagtgatact attatgaaac ttaagatcaa atctctatta aaaagtaata taataactaa
    11881 ataaatcaaa acacatatat tagaaagaat atttagtttt atttttttgt ttttaaatgt
    11941 taataaaatt ccaatattag caatggtaga ccagcttaat agcgcagtag aaattagcgt
    12001 taatcccccc caattttgta aattataaga ccattgattg gcaataaatc caccacctac
    12061 actagctatt atccataaaa tcacaagctc ccaccagttg agtttagata taaaaaatct
    12121 agcacataag tataacatca aaatatttaa tatcaattcc caccaattga cgtggataaa
    12181 gggatgtaaa agtaaagtaa aaaagaggct atcactatta ggcaataaaa cactttgcag
    12241 aatattatta gcagagctaa tagaaataat agacaataca gtagaaatga ttaaaattat
    12301 ataaatgatg agttttttcc aggatagcga aaacataata tttattagtt ttaataataa
    12361 aatttatatg gtttaaatcg atacccccat ataatcataa gtatcaagcc aaatatcatg
    12421 ccaccgaggt gggcaaaatg agctatacca ctatgaggtt gatatacacc tgcaaaaaac
    12481 tcaaatgccc catatattaa tacaaaaact atagctctga caggaagtaa cccgtatatg
    12541 aaaactggta cattaggaaa taacaaacca aaagcaagta gaatgccaaa aacagcacct
    12601 gaggctccaa ccattggtat attcatcatc atatataata agccttttaa tgattctact
    12661 gtgctaggag ctactaaagc ccaattattc aacaattcat ttactttcac tggatcatat
    12721 ctattagcaa aaaacttatt aagtatataa acaaattcat taggatctaa tgattgtgag
    12781 taagcagcaa tagctttttg catgctagta aattcaaaaa aattaacgcc tatttgaaat
    12841 aaagcagctc ctataccagt tacaaaatag aaggtcaaaa atcttttggt cccccataca
    12901 ttttctagca tatagccaaa catccacaga gcaaacatat tagaaaataa gtgcataaag
    12961 ctaccatgca taaacatata tgtaataaat tgataccatc tgaagtaggg tgaagctgga
    13021 taatgtaaac caagaatatt aattatgtct attcccctct gcataaaaac tacagtaccg
    13081 aaataaacta ttgtatttat tattaataaa ttcaatactg cgggagggaa taatgaaaaa
    13141 tgtgatggtc caaaaagtct ttgatcattc ataagattgt attaattgta ataatttatt
    13201 agttgacaaa attataaaat tatttttacc tcctatagct ttttgaggat cggttaaaga
    13261 aaataagttt gctagtaaag gatttaatga ttcagaattt agtttattta gttgtttttt
    13321 agctgcattg agtgccatat tataaaaaag caactcatta ttaagtggca aacctgcctc
    13381 tggtaattca agtaatctat aaataaaaga ttctatgttg tcttcatcta tataaccgtg
    13441 acacgggatg ccaataacct gaaattgatt attagaaatt ttttcaaatt tgaatccaat
    13501 ctcttcgagt aatgattgaa gtgacagaaa tgtccccata tatttgggtt ctatatttat
    13561 gattactgga aatagtacat gttggcaacc tgcatttata ttatttttgt ttgtaattaa
    13621 ttgatcataa atcaatcttt gtaaaataga ttctacatca cctatcaaaa taccatcctt
    13681 cattttagta attaatattt tattatctga ggacagcact acatctttat tattttcatt
    13741 tttttcttct aattcttttt ctatcataga gatttcttcg aagagttctc ggtaattttc
    13801 attgataatt tctttttgtg tatttatttt ttgataaaag agttttgatg tatcgctaag
    13861 aggtttatgc ggaaactgtg aattgatatt tgaatagctt ttatctgtat gaggcgagtt
    13921 aatagaaaaa atatgagtgg ggatagcatc gaaatctata cctggagtta gtccgctaat
    13981 acctaaggct tttttgcata aactatttag cgatgcagct atcagacgtt cgtcttgaaa
    14041 tttaatttct gttttactag gatgtacatt tacatctaag cgttgtggtg gtatatcaaa
    14101 gaaaatgaca taagcgggga tagcatcttt ttctattaac ccttcataag ctctgatgat
    14161 aacttggtga aaatatgggc tacggaaata tctgccatta gcataaaaat attgattgcc
    14221 gcgtgttttt ttagctgcat tcggagtaga tatatatccc atgattttac caaaaggaaa
    14281 ttcttcttcg tagtatataa gtttactttc gtagtttttg ccaaaaatat ctataatgcg
    14341 ttgcttgaga ctagtaggtg gtaattttat ttttaattca tcgttttcag tcaaagaaaa
    14401 agcaatatta ttattaggaa ttgcaatacg ataaaattca tcccatatat tgcgcatctc
    14461 tgtattatca gattttagaa aattgcgcct tgctggtaca gaaaaaaaga ggtcttttac
    14521 taaaatatta gttcctacag ggcatgccgt aggttctatt tttataatct tactattttc
    14581 tactaagcaa tgtgtaccta attctaaatc agcagtacga gtaaacattt ctacacgtga
    14641 tacagcagct attgacgcca atgcttcacc acgaaatcca taagaatata agctgtataa
    14701 atcttcgata ttataaattt tagaagtagc atgcctctca aaagctagtg atatatctac
    14761 gggatatatc ccttgtccat tgtctaccac ttggattaat gttttaccag cattttgtat
    14821 tattacatct atactactag cattagcatc tatagagttt tcagatagtt ccttcaatac
    14881 tgaagcgggt ttttgcacta cttcacctgc tgctatttga ttgattatct gttcctttag
    14941 tattattatg cctcgtttta tttcattcat tagtatataa ttgtaattac aaaaaaataa
    15001 tcactaacaa aagaagaagt aatactatta acgcagacca aatgttaata gaggttcgct
    15061 ttctagcttt tatctttcgc ttccatttct gatctagttc cctacgtaaa cgttccctct
    15121 ctagctctcg ctcatcgaga tctgataaat ctatatcttc tttgctacta aacctaggtt
    15181 tatattcaaa tttttttggt ttaggagtag ggaataaaaa actcatattt ttttacaaat
    15241 ttaacaaaaa aataaataat aaattaaaaa aagttactaa aaaatgattt ttgttgtttt
    15301 ttgcattaag gtaaaaacaa ttttattaat ttagcaaact tctaaaattt tagtataaat
    15361 gaatgaaata tacaaaatta aaaaaggact agatatacct atagatggtg aggcacctag
    15421 aatggaaata ggatttatgt taaaagaaaa gattgctatt ttacctcctg attttagact
    15481 tttcactcct agattactcg taaaagaaca agaaaatgta atagtggggc aaccaattat
    15541 tcaaaataaa ttagatgaaa acattatagt tacatctcct attagtggta ctataaaaga
    15601 aattaataga ggtgaaaaaa gggcaatcaa gcaaatagta atagaaccag acttaaatca
    15661 aaaatacaaa gaatttgata tccctaattc gttagatcgt gacaatataa tagatgtttt
    15721 attagtatca ggtttatggg tatatattcg gcagcgtcct tacggtatca tagctaatcc
    15781 tatagatata ccaaaggcta tttttatttc tgcctttgat acttcgcctt tggctcctga
    15841 tgtagatttt ttattatggg gtgaagatga aaattttcaa aaaggcatag atgttttaaa
    15901 aaaattaacc tcaggtccta tacatctcaa tattaatcct tcgaaaaata ggtcagattt
    15961 tttctttaaa atcaaaaatg tagaacatca ttttttcgaa ggtccacatc ctgctggaaa
    16021 tgtcagtgtg catatatcga ggatcagtcc acttaataaa ggtgatattt attggtacct
    16081 ttatcctcag gatgtaatgt ttataggtag attgtttaat actggtagat tagatttaca
    16141 tagaaaaata gctttgacag gtagtgaact tactgagcgt ggatatatca ctatactgcc
    16201 aggtactgcc atcaattcat tgatagcgga tagattaaag caggacaatg taagagtgat
    16261 tagtggtaat gttttaacag ggactaatgc aggtatcgat ggatatctca gctattatga
    16321 taatcagata accgtgatcc cagagggaga ttattatgat ttttttggat gggttgctcc
    16381 taaatttaat aaatatagtc cctctcgtat gatttttaat tggttagtaa ggaaaaaaga
    16441 ccaaaaatat gtactagata ccaatatgca tggcgaagag agaccttttg tattgagtga
    16501 gcaatatgac aaatattttc cctttgatat ctatcctgtt tatttactaa aagctatact
    16561 tagtcaaaat attgaattaa tggaagatct tgggatttat gaagtagtag aagaggattt
    16621 tgctctacca gagtatgtat gtgtatcaaa gataaatttg caagaaatca tgagaaaagg
    16681 tatagattta atgataaaag aaactaccta gtaaaataat gataaatatt atgataataa
    16741 gaaaaatttt aaaatctatg attaaaataa taaataaaaa ataaggcaaa caatatgcag
    16801 gtatatttct ctaacatttc taaatttgta tttagaaatt aaatattgca acaaatagct
    16861 taaaaaaaaa atatagtaaa attatattaa tatgaactgg ttaaggtcat ttttagacaa
    16921 aataaagcca aatttcgaaa aaggaggtaa actatatttt ctccattcta cctttgaagc
    16981 ttttgaagta ttttttttcg tacccaataa agtaacagaa aaggggtctc acatcagaga
    17041 ttatgtagat ttgaaaagat tgatgactca tgtactaata gctttggtac caatagtttt
    17101 atttggtatg tttaatgttg gcttgcaaca ttacagatta caattaggaa tagaaatcga
    17161 tgcttggggg caggtagtaa aagaggctgg tttttggaga atattttggt atggattttt
    17221 gagagtttta ccgatcatta ttgttagtta tgtttgtggt ttagctgtag agtttgcctc
    17281 tgcacagata cgacatcacc aagtaaatga gggttttttg gtaacaggta tgctgatagc
    17341 cctgatagta ccacctacta tacctttgtg gatgttagca gtagccacta tttttgctgt
    17401 catttttggt aaagaagttt tcggcggtac tggtatgaat tttttaaatc ctgctttatt
    17461 aacgagagca tttttatttt ttgcttatcc tgctagcatg acgggagatg ctgtatggat
    17521 tgtaaataaa ggattagatg ttattagcgg tcctacacca ttagcattgg ctgctcaaaa
    17581 tcaatttgct caagctcctt cattattgac catgactttg gggtttattc ctggatctat
    17641 cggtgaaaca tctaaaatat taatagtact agctgcattt tatttaattt tcactggggt
    17701 tgttaattta cgtattatta ttagtactct gataggcgct acagctatgg gattaatctt
    17761 taatctatgg ggcgctaacg aatatatgca aattccattt tattatcact tcttttttgg
    17821 tggattttta tttggtacag tttttatggc tacagatccg gttacttctg cacaaactcc
    17881 cataggtaaa atattatatg gtttattaat tggtatttta tgtatgctta ttcgtgttgt
    17941 caatccagct tatccagaag gagctatgct cgctattcta ttgatgaatg ttttcgctcc
    18001 attaatagat catgtcgtaa ttcagcaaaa tattaatcgt cgtaaaaaaa gagcattatt
    18061 atctcaaaca ataaaatcat aatcttatgt ataagtttac aaataaatat attatcatat
    18121 attcgattat aatggtagct atagtagctg tgctactaac tctaggtgcc actgctttga
    18181 aacctttgca agataaaaac aaaaaaaatg aaaaaatcgg atatattctc agggcttcga
    18241 gacaagagtt tgaccctaag aatgcagaag atacttacaa aaaattatta atcaaagaaa
    18301 tagcaattaa tgaaagcggt gacataattg cagaatttga ttatattaat ggtttacgag
    18361 gtaagctaag gcctttcgat attgatgtaa atacagaaac taaaaaattc aaagaaggca
    18421 aatcatatat tttgccaata tatttaatac aaaacaatgg cgatactctt acagtgatac
    18481 cattctatgg tatgggactt tggggagcta tttggggtta tgtagcctta gaaaatgatt
    18541 tgaatactat tcatggagct gtatttgatc ataaaggcga aacaccaggt ctaggagccg
    18601 aaatatcaga tttgccattt caaaatcaat ttattggcaa aaagattttc gatgaaaacc
    18661 aaaattttgt aggcgtagct atagttaaag gtggtgtgag acgtagtaat ataccagagc
    18721 tacatggagt agacgctatc tcgggtggca cattcactag taaaggctta gataatatgc
    18781 ttttggatgg atttaaaatg gctatgccct atatcgaaaa acatagaaat taatttttag
    18841 tattttttgt tttaatttag caataaagat attactacat cattaattaa ttacctattt
    18901 tttaaattag tataattatg aaaaacgaaa ataaagatat cgatgctaat catttgcctg
    18961 tgatggaaac attctatagt ctccaaggtg agggatatca tactggatta gcttcctttt
    19021 ttattaggct tggaggatgc gaccttagct gttggttttg tgatactaaa gagtcatggg
    19081 accctcatct taatgaatta acttcacctc aagatcttct cgacaaagct aaaaaatata
    19141 aaactaagca tgttgtgctc acaggtggag aacctatatt gtatcctttg caacttctaa
    19201 tctcactatt acataagcac gattatataa ttcacctaga aactgcagct acagcacctt
    19261 taataaatga aatagattgg gtatgtttaa gtcctaaaaa tcatactaat attcataatg
    19321 aatggtttca aaaagctaat gagttaaaag taataataac cgaggcaaaa gacttgaaat
    19381 ttgcagaaga gtgctctaaa ttagtaagta aaaattgtca tttatttctc caacctgaat
    19441 ggcatagatc taaaatatta ttaccacaaa taatcgaata tatcttagat aatcctatat
    19501 ggcgtctatc aattcagact cataaattta taaatataag gtaatttttt ttaactaaca
    19561 atataaaact gaaaataata tattctttta acaatagatg tgattcattg ataaaatttt
    19621 cggttctata acgttttata tgggttaaaa attaaaaaag agtaatttgt taatattagt
    19681 tcatttatta ttggacagtt aaatattaac cacagagtac tctaataatg ttcaaagaac
    19741 acagaatagg atggaattaa aagggctaca aatagctctg tgtaataaaa aaattaacct
    19801 aaataattaa aaaattaacc taaataatta aaaaatagtt atttttttgt aacaatctaa
    19861 aatttgctta cccaaattat aacctaaaat atgttggatg tggagatttg gaaacttgaa
    19921 acggatcagt cggaaaacta atatatgttt caattaacat atgttatatt tactaattct
    19981 ctataccgat accatgccca tctatccgag gatgcgaggt tgagcattcg cccagtattg
    20041 cttacagtct tgcctgtgag gtatttaccc atcactacat accacataat gtagttttgc
    20101 aggtatttag tagctacgcc cctaaatttg aaatatatcc aattagtgaa ttcactagcc
    20161 tttttcttga cagtacttag gctatatatg cctcgctttt gcctatggct agttactact
    20221 tctttatgct tgattacatt ccttctaacc tttttgaatg cttttttatt actgctacac
    20281 atgacaccat cctttggcat tttagccttc agaaattgct ctatttgttc tgaacttagc
    20341 tttttgcctt caaatttttt gaataataaa ttgccagctc tgtctgtagc agccaatacg
    20401 gctactttct ttggcttttg cttcctagct cgcttcagct cttcggcatt tttgtatatt
    20461 ctgccttttt cggaataatc catcaatagt tcgtccatct ctactacacc gaaaaaattg
    20521 atattatctt cgaaactttg tagagctgcc aatattttgt gtcgccaagc gaagcttgtt
    20581 ggtaagctga taccaacctc tttagcacaa gctctgagac ttttgcctgc taccatacat
    20641 ctgatgtaat cgagcatttt ttctttcttt tgtaacctgt agacaaaagt accggtggta
    20701 actctaaact gtctgccaca gtctttgcag cgatagtttt gcatcccatt ttgtaagcca
    20761 tttttaatga tgtgattgct acgacagtct gggcatagtg gcggggcact gagtattttc
    20821 agaccttgat aatcgactac atcggcttct aattttttca ttagagaata aagattgtct
    20881 aagaattctt tattctcttc cttactcata gcaagatatt tgcttgtgtt ttccactatt
    20941 tttttcatga attaaaattt ttgtaaaaat ataaaaatta tttaacatat aaacttgaaa
    21001 aacttaaaca tttattagtt tttcatctaa tttcttttat tcgctattat ttaatacaac
    21061 ccgaattagc tttataatcg taattatcgt atctcttaat gatttttttg taacaatcta
    21121 aaatttttac ccacaataaa cattatagaa ccaattttta tatctcttgg catcattgta
    21181 cttgataatg attcaaaaaa aattcaaagt tttggtttaa aaatcatttt gcaataattg
    21241 tttgactttt tctattattt cttcttcact acctatggag taaccagtat gaatccaaac
    21301 aatatcacga tttttatcta aaataaaatt atgtgggata ttatttactc ctaataatct
    21361 ttttagatca tgattgggat cttttaaaat gataaatggc caatttttac cttttacaaa
    21421 gctagagaca gtcgcacttg tacgttgatc atctatactg atagcatata atttaacgcc
    21481 tgtcatttct tcccattcat catataaatc agagattgcg tttagttctt gaaggcaagg
    21541 tttacaccat gtagcccaaa aactgataat tatcggtttg ttttcatttt taatggaatc
    21601 tgtattaaaa acattaccat tgatatcaaa aatatttata gaaggcaatg gcttttgagc
    21661 aaaaataaaa ttgcttatta gcaaacaaaa aaagagaaat aatttattca taagtaaata
    21721 atttaatttt taacaaaatt atataatatt cttaaaataa taaaacataa attagcatta
    21781 cattaaaatg tcgaaaaaat aattaatttt gcaaataaaa aaaagtaaaa atggctaaga
    21841 tacttattac aaatgatgac ggatatagag ctgcaggttt aaatgctttg atagagttta
    21901 ttgatccgat agctgatata gtagctataa gctctgagac agagatgtct ggtagcggac
    21961 atgcagtcac tatcaaaaac cctttaaggc ttcgcgtgca tgaagaccat tataaatcaa
    22021 tttatgtatc taatggtacg ccagcagata atgtaaagtt agctgttaat tgtatatttg
    22081 ataaatttcc agatttaata ttaagtggta ttaatcatgg atccaatgct tcgtgtaatg
    22141 ttttttattc aggtactttg gcagcagtta tagaaggagc catgctagga ataccatcta
    22201 ttggtttctc agttgattca tattacaaag gatatgattt tataattata aaggattacg
    22261 taactcatat tgttaaaaaa gctttagaat ttatttcact acataaagaa aatattttat
    22321 gccttaatat aaattttcct gctatagaaa atcctagggg tatcaaagta tgcaggatgg
    22381 ctaaagctaa atgggtagaa agatttgaaa aacgaataga ccctcatggt attccttatt
    22441 attggttaga tggtgatttt gtgtttactg atgaaggcga agataatgac gaagctatag
    22501 taaagcaagg atacatttct cttacacctt tatcattaga tatgacagat tacaatatta
    22561 tggattatct tagatcttgg gaattatgat tcgaaatttt tttaaacggg atagttggag
    22621 cttaggtata attttaggta tagtagtacc attaattatg tttggccttt tagctgctat
    22681 agaagcttta tttagcactg gttctgctga tcatattttt agattatcta ctagaattgt
    22741 aatttcagtt ttttgtaatt tacttccttt tagacattat atggtcaaac taaaatttga
    22801 taagacaggt cgtggtattt tactagatac ttttattttt gcaattatat tttttatttt
    22861 cttttttagt gaataaaaaa ttgataaaaa aaaacaagaa atttagaagt ctaaatttct
    22921 tgttttaatc tattatttat atctcaaatt tttttattac atattcatta agatgttttg
    22981 tttttatatg aaattatata tagatgaaat atatttatgt ttttttttat taagaaaata
    23041 gctcttcagc tactagatca tcaataaaat cattaaaatt atttaataaa tcttcttcat
    23101 tactcatatc taccaccgga atgcttagag ttttagtgaa ttgcaaaata tcggaattta
    23161 tattttcatt cataataata gcagcatcac tatgcattag agaatctttc attagattta
    23221 tgtaattagt atcttttaga agatttggga ataaatcact tgttaagtca tcaaatatta
    23281 atttatctac agtatcatca gataattttt cttcgaattg atcattatgt agagctaaaa
    23341 ccacttttga ttcactaaat aaaggatgtt tagaaaatac atatttgacc ataggagcta
    23401 ataatgcact catccatcca tgtaatacta ttacattagg agaccaacct aattttttaa
    23461 tactttctgc cacacttcta gcaaaaaata ttgctcttgt agaattatct tgaaaaaaat
    23521 tctttttatc atctctaaat ataaattttc tttgaaaaaa ttcttgatta tcaataaaat
    23581 aaacttgaat atttgtatta ggcattctag ctacttttat tattaaaaga tgatcattgt
    23641 tatcaattat tacatttatt ccagataatc ttataacttc atgtaaatta tatcttcttt
    23701 catttataca tccatatctt ggcataaata cacgtacatc tttaccatca tttgaaactt
    23761 tttctggtaa tattctacta atttttgata attcattttc ttttacaaaa ggataaattt
    23821 cttgacttac aaataacact cttgtttttt ccatttttat tattttttat aattatattc
    23881 gtatatgcac aaagatacaa aaaaaaattc aaatggattt tttgaatttt tctttaattg
    23941 ataattgtta attggcattt tgtaataaaa tatatgtttt atatcttaat tatctttata
    24001 ctaagttttt tgattaataa aaaatttctt ttatttttgc aaataagaaa cgtgtaatta
    24061 tgaaagcatt tgtttttagt gttaaaagtc tcaatgcaga ggatttcgaa attataattg
    24121 aattattatc taatcagaca tttgaagatt tttttattgc tctcaaaaat gctattggat
    24181 ttactgataa cgaattagct tctttttatc tatgtaatag tgcgtggaaa ccaaaaaaag
    24241 aaatagcact aatagatatg ggacaagatg atgatgaaaa taataaagta ttgattatgt
    24301 ctaaaaccaa gttaaacatt atcgaagatc cgcatcaaaa gatacttttt gtctatgatt
    24361 atataaaaaa tcataaattt aatatagaac ttataaaaat taaagataga gatcctgata
    24421 ttgaatatcc aaatatagtt aaaattaaag gcaaatatat aaaaacttta acgcccaata
    24481 tacctatacc ggtcactact gatgatgata ttttagatga agaagatgat gaggaagaaa
    24541 tattagaaga gttcgatgta gtagttgata atatatctga agatgaaaat gatacagatg
    24601 atattataga cgaacatttt tttgaagata tagcagataa gactttagat gattttgaaa
    24661 atgagtaaat ttaaagatag cttgcaaaaa taatttataa accttgaaaa ttataataaa
    24721 aaattatgaa tactttagaa attatagata aagaatcaaa agataaaaat attttaataa
    24781 ttggtgatgt gatgttagat aggtatatat ggggcgatgt agcacgtatc tcacctgaag
    24841 cacctgtacc agtcgtaaat gtaacaaaaa tcgaacatcg cttaggtggg tcagccaatg
    24901 tagctcttaa tattcaatca cttggatcta atccctttct agttggagtc ataggtaatg
    24961 atgctagtgg taatgaattt ataaacctta tgaaatctca aaaaatgatt acagaaggtt
    25021 tgataactgc ctctgataga caaactatta ctaaaacaag aataatgggc aataatgttc
    25081 agatgttacg tgtagatgat gaaaatagca aaaaccttcc agactcttta cgccaacaag
    25141 ttttattgaa aattactaat ttattagaaa aacacaaaat tcatgcaata atttttcaag
    25201 attatgacaa gggcgtaata gatcctttat taataaaaca tattgtacaa ttatcaaaaa
    25261 cctatgatat accagtatgt gtagatccta aaaaaagaaa ttttgatttt tataataatg
    25321 ttactttatt caagccaaat ttaaaagaat ttagagaggg tataaactgg aatgatgaag
    25381 catttaacat gaatactctt tttgataaaa ttcatcaatt tagagaagct aaaaatatta
    25441 aatatatgat agtaacaatg ggggaaaaag gccttgtact ggcaaaaaca aatgagatca
    25501 ataatacttt ttatactatc agcatacccc cacctaagat acgccaagta agggatgttt
    25561 ctggtgctgg tgatacagtc attagtgtcg tagcactagg gttagcatta ggcttaaata
    25621 tagaattaat tgcagagctt agtaatttag ctggtggcat tgtgtgcgaa tatgtaggag
    25681 tggtaccgat tagtaaaaaa caactattag aagaagctgc catctattta aaaaatgtaa
    25741 aatgattttt tatctttact aaaagatgaa ataataaata atcttatgat ctcattaagg
    25801 atccaagatt taaagaattt ataaagtatt aaattttttg tttttatttt tttgctgcct
    25861 tttccagttt tagatctcta gttatcattt tcactcatac ttcgccactt attattgctt
    25921 taatattttg atttgtttaa ccaattaata tagctaatat aagattagct tattaatcta
    25981 taagtaataa ataattaatg ggttgtagca gattcgaact gctgacctca tgcgtgtgaa
    26041 gcatgtgctc taaaccagct gagctaacaa cccaaaaaaa tcatttttga ataaatttat
    26101 taccatcgta tgcccatttc atgtatatag ctccccaagt aaatcctgca ccgaatgtag
    26161 ctagaataat gttatcaccc tttttaagta gatgttcata atccattaag cataaaggca
    26221 aagtagcaga agtagtattt ccgtatcgtt ctatattgat cataacttta tctttaggca
    26281 atttcatccg atgagctact gcttctatta tacgaacatt agcttgatgt ggtactagcc
    26341 aatcaatagt ttcattagtt agattatttc ttttcattat ttctacagat acgtcagcca
    26401 ttttagaaac ggcccattta aagacagctt gcccttcttg atatatataa tgctctctat
    26461 tttttacagt ttctattgtt gctggccata cactaccacc tgctttttga tatagatgat
    26521 gtctacctac accatcagat tgtaatatca tatctataat accaagatca tctccataat
    26581 aagcttccat taatacagct ccagcaccat ctccaaaaat aggacaagta gtcctatcag
    26641 tataatcagt aatagaagac attttatcag caccgacaac aataattttt tttgcatgtc
    26701 cagcttctat atatgctcta gctatagcta acgcatatag aaaactacta caaccagcat
    26761 tcatatcaaa atgaaaagca ttctttatac ctactttgtc tgcaattata ttagctgtag
    26821 ctgggaattg atgatctggt gtgatagtag cacataacaa aaattcaatc tcatctggcg
    26881 atgtatttgt cttttcaaat aacttttgta cagcttgttt tgctaattca ctagtacctt
    26941 tgcctggctc ttttaaaatt ctacgttctt tgatgccgat gcgagtcata atccattcat
    27001 cagaggtatc gaccatgcga ctaagctctt cattagtcaa aatgtaatca ggtaatgcac
    27061 cagctacacc cgttatgaca gctctaggca ttgttctcat tactcttttt ttttaaaatt
    27121 attcattcct ttcaattgct aatttacctc tgtaataccc acattcagga catacatgat
    27181 ggtaaatcat aggactacca caattgggac aagttactcc tttgacagtt ttaattttat
    27241 actgtgaacg acgcttacgt gttctacttt tagaaaattt acgttttgga ttagccataa
    27301 ctttatattt ttgtattgat ttttaataat cttctattaa atattctttt aatttgttta
    27361 acatctcttg atcacaatca gattcatcat tatgcacatg tctcataggc atagataaag
    27421 ctataaaatc ataaatatgt ggtcgcaaat caataaattc agtaccttgt gagatataga
    27481 taatggtctc atcatcttcg cctatacctt ctttgaatat taaataatca ttcttatcta
    27541 tatctatttt cataaaactt aagcatctat cgcataatac attatatgaa ccttttaaat
    27601 ttataaataa ttcagcacac gtctcagttt ttaataaatc tatttttata tgaataaata
    27661 catctctcat ctcatcaaaa ttggggttat aatcatgtat aaatgtatca tctatatcat
    27721 agtcataata agaatgcccc aaattaagtg cttgataatt gattttataa tctaatttgg
    27781 taatcattga aaaaactttt ttaaaatttt tttgcaaatt taagtaaaaa ttataaaata
    27841 aaaatttcag ttttataata gaatttaacg tttatacaat taaattttta tttttattca
    27901 cggattcagg tgatgattta atttaaatct tttattacag atttaattta ttaaacaaat
    27961 ttcaaaaaaa ctgtatcttg gtctaaaaaa ttcattaatt tagtaaaaaa tcaataaaaa
    28021 tgtgcatata taagatattt tttaaatcaa aaaatgaaaa tagaaaaaat tctaataggg
    28081 aacaaaattc agaaataagt actaatgata taataaatga tttagacata aatgatgata
    28141 aagatattga aactttgttg atgaaattat ctggtagagt taaagaaaca aatacaaatt
    28201 taataaatag aggattaaaa gaaatacttt ctgattatag ttttgatgat ttaaaattag
    28261 atgataaatc taaaaagaaa attttggagt taaaagaact tgctaaaaac attgaaaata
    28321 aaatcataaa agagcctaat tatgatgcaa caaaaaatta taaaattgat tatgcaaatg
    28381 aattgaataa tgaacaatta atagctgtga ctacaactga tataccatta ttagtgatag
    28441 ctggtgcagg cagtggtaaa actagagtaa ttacctacaa agtttgttat ttaatagaaa
    28501 atggtgtaaa tccaaatagt attttattac taacttttac caaaaaagca gctcaagaaa
    28561 tgctagatag ggttaataaa ctattaaaaa acaaaatagg tggaaatgtt ttgggtggaa
    28621 cttttcatag tttcgcaaat tatattctta gaagatatag taaggtaatt gggattaagg
    28681 ataatttcac aatcattgat acaactgatt cagcagatat tttcagtcta ctaaaatctg
    28741 aattgaaaat aaataaaaaa attgatggga agcctttccc tgataaaaag gaattacaag
    28801 aaataacaac aaaagctaga aatatagaaa catcaattaa taatgtgata gataaatttt
    28861 tttataaata tattgattat acggatgaga ttataacatt aattaaatat tatgaaaaat
    28921 ataaaaaaac ttttaattta ttagattacg atgatttgat aattatttta agagataatt
    28981 tgcgcgacaa cgcaggtttt ttaaaagctg tacaaaatga aatagaatat gttttagtcg
    29041 atgaatatca agatacaaac actatacaaa gagatatagc tgatttgatt gctagtaaaa
    29101 atgaaaaaat aacagtagtg ggcgatgatg cacaaagtat atatacattt agaggtgctg
    29161 attatgaaaa tattctcaga tttccacaat ttcatccaaa ttgtggtgtt gtaaaactag
    29221 aatgtaatta tagaagcaat caaaacattt tgtattttat caatagtatt atttctaatg
    29281 ctcagattgg ctataaaaag aatttgtata caaatatgca atcaagccaa aaacctatat
    29341 ttaaaaaatt tgcaaatgaa agagatgaag caaaatttat tgtagacaaa attctagaaa
    29401 taaagggtga tattcttaat tataatgatt tcgcaatttt gacacgtgca gcatggcatt
    29461 caaatgtgat acagactgaa cttatggcta ggggtatccc atttgatgtt attggaggta
    29521 ttaaatttgc tgaaagagca catgttaaag atattatagc ttttttaaga atcattatta
    29581 atcatattga tgctatatct tggcatagaa tactgcaatt aatagatggt attggcaatg
    29641 tacgtgcaac agaaataata acatctatta ataaaaaaaa tggtattatt gagttttcag
    29701 aatttcaaaa tagaaaatat tacgatgaac taaatagatt agaaaatatg tataataaaa
    29761 taataaataa cgataatata aaaatcgatg aaatttttaa tttgatttac gatttttata
    29821 ttcctttatt taaaaagaat tacggcaatt atgatgaaag agagcaagat ttagaagttt
    29881 taagaattat tactagtaat tatgaatcaa tagaaaaatt tttagacgat ttcactctag
    29941 agcctcctaa caataaattt cataatggag tattccctgg atctaaaaat aataaggata
    30001 cagtaaaagt ttccacaatt cattcagcca aaggattaga atggaatact gtatttatac
    30061 cacatgctat tgatggactt ttcccctctg aaaaaagttt atcaacctta gaagatttag
    30121 aagaagaaag aagagtcttt tatgtagcat gtagcagagc taaagaaaat ttattcataa
    30181 ccattccagt atatttagaa agttatagag gatataccaa taaaccttca agatttatct
    30241 atgaaattga cccaaaattt tattttattg aagggaatga ttaattttga attatataaa
    30301 actttaatct gtgagcaaaa atttataata ttacttgaaa tgttgtagtt tttttattga
    30361 aaaaatttta tttttattca cggattcagg tgatgattta aaaaaatttt ttgcaaatat
    30421 caaaaatcaa aatggcagag ataaaatgaa ttaaattcaa aaatataaaa ttaccatggc
    30481 agattattca attacatccg ttaaaaagta aaattacaat ggtaaaatat aaaattataa
    30541 atcaataaaa tataattgca cttataaaaa agatttttac aataataatt taatgagaaa
    30601 gtaagtaact gattttattt ttattatggt gattgggcgt atggtaatac gtttctatga
    30661 tgagtttttc attggggcga gtcgtaaatt gctccaacaa tgaggttttc atgaaagcga
    30721 cgaatttagt tgttgcttca aaaataggat tatttatttt ttttgtataa tccccttatt
    30781 atattatttt tttctttttt tagaaacttt atcagagatg atctctgtgc ctaccactgt
    30841 aattatacca gctaacatca atagcaaagc tacgaaaaat tgattatcta tattggggag
    30901 gtaatattgg tattgtacaa tgtaaggttt gaaattaata agatcgccgt tagcatttat
    30961 aagtgcgcca tatttatttt gtaaaattag cccgtgagta tgatctatgc tatatttcca
    31021 aggccatatt agagcgagag agcctatgac aaatcctgct aatacactca aagtttcatt
    31081 ataaaatttt ttaagtaaaa tagaaagaac ataagagatc aaaatcaaac ctaagataga
    31141 gccaattgca aaaggtatta atatttttag atttaatgtt gtgacagcat ctaacataat
    31201 tagtttataa ttacctaaga ttaaaagcag ataagagcca gatataccag gtaatatcat
    31261 gccagatgtg ccaattatac cacataataa tatataaaat agattatcat tttcatttat
    31321 gggactcata aataccatta aaataggtat agatattcct attaataata acaaccaatc
    31381 ccaccatctt agtgatttca aaccttttat tattatccag gccgagccta ttattaatcc
    31441 aaaaaagtat gcccatgtat agataggata gtgcaaaaaa agatatttta gtaaaaaagc
    31501 tactgaaaat aaagctatca atacgcctgt aaagacttgt aataaaaaat aaaaatctat
    31561 atattcgatt aattctttta ttttgccttt gaataatagt ttaatagcag taaaattaaa
    31621 attttttagt gcatttatta atctttcata tatatttgtt atgagtgcta tagtgccacc
    31681 agatacacca ggtataacat ttgcagtgcc tatcgctatg ccttttaata ttaaaactaa
    31741 gtctaccata ttaaatttta tttattttat tcaaagattg aaagcaaaat tgttaaaaaa
    31801 atttcaaata ttatacatat ttattacata gccaattgat ttttcttttt taaaataaca
    31861 taatatcctg tagtaataaa atctctaaaa aatggtattt tattaaaagg aaaaaaaagt
    31921 tttaatgatt tcatattaaa tttaatttta aaagctggat ttatgatata atatctttcc
    31981 catattatat ttagattgct atctttgagt gtatgtttaa acttttctgt tgttatcctg
    32041 gtatcataaa tttctaacaa atcatttata attatttcat tttcaccaaa taattgcaat
    32101 aattttttat aagtatggta tggcaaaaga tgaatataag gtaaatgact taaaattttg
    32161 tttttacata tttgttgatg acctccaaaa ggactaaacc aaggtgggaa atttataaaa
    32221 atcattccat tgtctgataa aaatctatct atgtttttta aaaaattgtt ttggcaaggt
    32281 aaatgctcta atacatccct tgcaatgata acatcatagg tgtcattagg ttgcaaatcg
    32341 caaacatcac cgacaatcaa atggggagca ttgtttaaat taaaagtttc atatatcttt
    32401 ttaccatcag ttattcttga gctactgata tctatacctg tgcaagtcca ttgtcttttg
    32461 acaaaaggta aaagattgcc gccttctccg cagcctattt ctaataattt ttgtttttta
    32521 ttaatatcta taaatgggct taataatggt attattactt tttcacatac ttcagcttgc
    32581 attttgaaat aagctatgcg atcggaatgt cttttttgca tttttttgca aatttatgtc
    32641 ttttcaatta ataatattga agatcgtaaa attattttct agtgtttatc ttttaagaat
    32701 acattttttt ttaataattt taataatata tttgcattaa aaaaaaaatg aatttatttt
    32761 caaaaatcag agatgcgata ttgcgtagaa tatctaaaaa tatacaccct acaatgatat
    32821 taggatacaa acaaaatagt gagtatctcg atgatgtacg tataagtaat acaactgtaa
    32881 taaatgctag agaaaattta aaaatagcta atcatgtttt cattggacat tataatttca
    32941 tagaagctag taatggaata ataatcgaag agggttgcca gatttctaat tttgtgtcta
    33001 taaccacaca ttctagtcat atttctatta gactctatgg agcagattat acaaaataca
    33061 aaaatctata tgggtatgta actggtccta ttcatatagg taaatataca tttataggcc
    33121 ctcatagtgt tataatgcca aatactacaa taggtaaagg tagtatagta actgcatttg
    33181 cttatgtcga aggagaattt cctgattttt ctattatatc aggcaatcca gctaaaataa
    33241 taggggatac acgtcaaata gataaaattt ttttagaaaa ttatccagaa ttacaaattt
    33301 tgtatgatca atgggcgaga taaattatct agattaattt ttgtcaataa tgataaaaat
    33361 ttaagttaat ttacggagga caattagcac aaaatgaagc gaaaagattt gtactaaggt
    33421 atctatcacc tctatctggg aatatagtaa ctatatttcc agatttaatt ttttctgcta
    33481 atttgagagt acctgctagg gcagctccac tagacatacc tgcaaataca ccttcttctc
    33541 ttgttatttt tctagctaat tcaaaagatt cattatcatt tacataaaag tgcatatcaa
    33601 atagagagga gtcgtagata gatggcacta tagcttcttc taaatttttc aaaccttgaa
    33661 tcttatgatt ttttacaggt tctatagcta cgatttttat attttggata ttttctttta
    33721 ggaatttccc tgttcccatt aaggtacctg tagaaccaac accacaaacg aaataattta
    33781 tttgacatgg catttgattt agaatttctg gaccagtagt ttcgtaatgt gattgtggat
    33841 ttgctggatt agaaaattga tctggcataa aatataagtc tgaataatca ttcaataatt
    33901 tcttagctct gattatagct ccatcagtat tttctttagc tggagtgagc actagtttag
    33961 cgttataaga ttcgagaatt tttcttcttt ctaaggaaac acattcaggc ataaaaagtt
    34021 taattttgta acctttaata gcagctatca tagctaatgc tattccagta ttgccgctgg
    34081 tagcctctat tattgttttt tcttttgtta atattccttt tttttcagca tcatttatca
    34141 tgtataatgc aggtctatct ttaatagatc cacctggatt ctgtccttct attttggcaa
    34201 atattctaat ttctggaaaa tatggattta attttttaat ttctattagt ggggtattac
    34261 ctatgtagtc taaaatactc gtggtcatac gatatttttg agagttaatt atttttttga
    34321 tagttttcta ctccagtaat tgtatttgtt ccatcatttt cattaaaaaa taattcaaat
    34381 gtatgttctg taatggatgt tatttgacct gttccaaaaa atgtacgaga aatataagga
    34441 tctccacaag taattgattg actatttatt ataatactgg tatttactgt aatgggctct
    34501 gtaaaatata attctaaggt acaatttgta taattgcgaa atttattaac gtttgcatat
    34561 ttgttttttg tttgagaaat agaaatatta tcggtataag tgtatgtatt gccactagca
    34621 ttatcagtaa ctgtatatgt accagcccag gtatcagctt cattctttac tctgactatt
    34681 ctagttttaa tagacgtgtt gccattagaa tcagaagctt tataagtaag tgtataattt
    34741 ccaactaaat ctttgtttac atttcctgta acagttacag gtaccacacc atctacatca
    34801 tctatagcgg tagctcctgg atccatccat tgagtgttta aagctataga agtatctgag
    34861 gctccattta aatatattat tggcggggta gtgtcgccat tggtagtatt accattatta
    34921 tcattttcat cttcacatgc tacaaaaaac gtgctcaatg agattaataa taaaattata
    34981 aatgtttttg ttttcataat atattatttt gattgttgac aaattattat tgcgaattta
    35041 tagattttat tttttttaat acaaattttt taatttcatc aatcagtatt atataattgt
    35101 ctttattttc gcctcccata atagctatgt taggttgtcc accaccttta ccattaataa
    35161 ttggtgaagc aatatttatc aaatcttttg tggtaaaatt atattttttt ataacttcat
    35221 ctgctaaaac aaaagctaca ttagcttttt cagatttact agaagctagt actgctatta
    35281 tattcttatt aaatgttcgc aaaataaaag atagtggtcg catttgatct gcagttatat
    35341 caattatttc tcctatgaaa tgcaaattat caattttttc tgctctctca attaaattgc
    35401 gagcaatatt attcaatttt tcctttatta attcgtcatt ttgtttggtt aattctttat
    35461 ttttttcgat caaattttct acactttcta aaggtctatt ggttttcaaa atagatttga
    35521 tatcagataa aatatcataa gtttcttgaa aaatttcaat agctttcatc cctgtaacag
    35581 cttctatacg tctaatacca gaagctatag ctgtttcatt gattattttg aagcagccaa
    35641 tatcacctgt agctttcaca tgagtgcccc cgcatagctc tttgctatca ccaaatttaa
    35701 tcattctaac acgttcatca tatttttcac caaatagagc tatagctccc atgtctattg
    35761 cttgctgata tgagatattt ctatgttctt cgagtttata attatcttgt atcaacttat
    35821 ttaccagttt ttctatatca tttaattgct gacgagataa cttttcataa tgagaaaaat
    35881 caaatcttag ataatcggga tgcacgaggg atcctttttg ttctgcatgt ggccctatta
    35941 tttgcctcag agcatgatga agtaaatgag tagctgagtg atttctcatt gtagaaaatc
    36001 gtttttctac atctactttt gcaatgaaga ttgattgcaa atcttgtggt agtttctcag
    36061 ctatatgaat gatctctcca tattctttaa tagtatttat gataaatata gtttctttat
    36121 cagaatagat gattccacta tcacccactt gtccaccact ctcagcgtaa aaaggagttt
    36181 tatctaaaac taaatgataa aaagttttat ttttttgaac tgttttgcga tatttaatta
    36241 tttttactgg acattctaat gtatcatatc ctacaaattc actttcacct tcatttatta
    36301 ttatccagtc tgcagtatct acttgcgcag attggcggct acgctccctt tgtatattta
    36361 aaaattcttc gaattttttg atatcaacag atagaccttt ttcttttgcc atgagttgag
    36421 taaggtcgat aggaaagccg tatgtatcat agagttcaaa agcaaactgt ccatcgatga
    36481 ttttagcatt tttattgttt tcgatatatt gttcgaaaag tttaatgcca cgctcaagag
    36541 tagagaggaa ggtgatttct tcatgtttga taactttttc aattagttca gattgttgtt
    36601 ttagttcagg aaaaatctca caatagtgtt ctataaaagt ttgactaatt ttatgaataa
    36661 aaggttcaga aatattcaaa tattgatagc catatctaat agcacgtctg aggatacgcc
    36721 taatgacata tccagctttc acatttgagg gcaattgccc atcggctata gcacagctaa
    36781 ttgctcttgt gtgatctgct atcactctaa aagctatatc tttttgtgga tcagctttat
    36841 aaggtattcc tgataattgt tcgatttttt taattaatgg ttgaaaaaga tctgtatcgt
    36901 agttagagct tacattttgt agtactcttg tcaatctctc taatcccata ccagtatcta
    36961 cgtgcttttt gggaagtaga gatagcgatc catctgcatt tctattatat tgaataaaaa
    37021 ctaaattcca caattctatt acttcaggat gatttttatt tactaggtta gctccaactg
    37081 attcttttct ttcttctttg ctacgcaggt ctatatgtat ttctgagcag ggaccacatg
    37141 gacctatatt acccatttcc cagaaattat cttttttatt accaggtaaa acatggtcag
    37201 ctggcaacac actgagccac acttttttag cttcagtatc ttcatcgaga ttttcggctg
    37261 tatcaccttc gaaataagta gcataaagtt gatcaatatc gattttgtaa atatcagtca
    37321 aaagctccat tgcccaatga atagcttcat ttttaaaata atcaccaaag gaccaattgc
    37381 cgagcatttc aaacattgta tgatggtaat gatctaggcc tacttgctca agatcattat
    37441 gctttccact cactctcaag catttctgag tattggctac acgtttatat ggcggctctt
    37501 gtaaatctaa aaaaatatct ttgaattgat tcattcctgc atttgtaaac atcagagtag
    37561 gatcattttt tatcacaatg ggtgcactag gtactatgat atggtctttt tttctaaaaa
    37621 agtctaaaaa attctttcta atttcataac tagtcatagg catagtaaaa aaatttttac
    37681 aaagttaagt ataatattgc aaaatattaa tatgtgaatt tgaacaataa atttgaatca
    37741 gaaatcttaa ttaaaaaaaa taatataaat ttgctaaaaa taattaaaat taatattttc
    37801 ctatgaaaaa tgaaatggaa ctaaatttat ttgaatttaa tgaaaatgat gatttgaaaa
    37861 aaaatgtttc ggtatatttt aataaagaac ccttaattaa agtactagat gattttgagc
    37921 aaatgaatga tataaataaa attaaaaaag aatttttata tcttattcaa ataattgaaa
    37981 atccaaagga agatcgagtt ggattaataa gcattaataa taaaggagat ataattgaat
    38041 gtaataaata ttatataata accgaaataa atacaatttt aaaatcccaa accattgaac
    38101 gtattcatta ttatattaaa agactaaaga aaagtttatt agaagtaaaa accaataaaa
    38161 taaatgatct taatttaaat caatggaaaa aatatgataa catattaaca gatagcttat
    38221 ggatattaga taaaagagat agttctgggg ctcataatgc cggctattgg ggtaatttca
    38281 taccacaaat accaaatcaa tttttgcaaa ggtacactaa aaaaaatgaa tgggttttag
    38341 atccatttct tggaagtgga actactttaa tagaatgtaa aagattgggc aggaatggtg
    38401 ttggagtaga gctattgcca gaagtagtag aattggctga acataacata tcattagaaa
    38461 caaatattta taatgttaaa acagaaatac tcaacgcaga tagtactaaa ttaaatttta
    38521 aagatgaatt aaaaaaatta aatatttctt cggttcaatt tttaattatg catccgccat
    38581 actgggacat tataaaattt agcgaaaatc ctaatgattt gtctaatgcc aatactattg
    38641 atagtttttt agatttatta ggcaaagtag ttgataatac ctatgatatt ttagacaatg
    38701 aaagatatat ggcattagta ataggtgata aatattctaa aggtgaatgg atcccacttg
    38761 gattttactg catgcaagaa gttttaaaaa gaggatatct tttgaaaagt atcattgtaa
    38821 aaaattttga agaaacaaaa ggtaaaatga atcaaaaaga attgtggaga tacagagcat
    38881 tagcgggcgg attttatgtg tttaaacatg aatatatatt tttatttcaa aaatcaaaaa
    38941 aatgacaaca catgttttta ttgtagatat aaatactttt aagtaccatt tagaatatct
    39001 ttttgcaggt acaggagccc aagatcttta tattgacttt aataataatt ctaattcttc
    39061 attaaattat gctatagaaa ataacttagt tagtatgatt tcagattctc agaggataag
    39121 aaagggcgat tatataattt tttatttaca acaaaataaa gctaaaaaag tttatgaagg
    39181 taaattttat ggtattttta aagccaaaga taatcattca tttcttgata ataatgataa
    39241 aaatcaattt ttaaaagata atttaaacaa atctctaact tttagaacta taatagaacc
    39301 atacaaagta tatcctatag gtgtgacaga atgggaagct ttagatgaaa taaaatatat
    39361 acaatcacct aatcaaatgc tttggagttt aatttataga aaattacgtg ctaatagagg
    39421 caatactatg atcacaattt atgaaagtga aaggctgatt aaattaataa aagataaaaa
    39481 taatggcaaa acattaaatt gcaataattt tacttttgat actaatacac aggaaataat
    39541 tggaaataat gcaaaatatc cttatactgg gagaaaagaa aatataaata ttttaccaag
    39601 attaattaat aaatttaatc agggtaaatc ttttgaacct catcttcagg catatatagt
    39661 gcaaaatata ggtagaaaaa cgcataataa tctagataat ttattagtta ataattatga
    39721 tgtcgaatgg attgggaatg aagtatattg tggtgtggga atgcaaaaaa tagatataat
    39781 gctatcttta ataaaagatg atgaaagaat aattgaacct atagaattaa aatctgtatg
    39841 tgctacacca gatattattt ttcaaattca gagatatata gattggatag agcaatacta
    39901 tattccaaat agaataagtg atattcaacc agttataata tctaaaaaaa tatcaaataa
    39961 acaatcatcc aactattccc tattaatcaa taattttaaa aatcttaatg ataaaaatat
    40021 aattttacct ctaaagtata tagagttcga aatcattaat aacaatataa ttttcaaaga
    40081 aatactttac taaagcctat atattctttt ttatttgata aattttattt atttttgtaa
    40141 aaaaattata cggctccgta gctcaactga atagagcatc tgactacgga tcagaaggtt
    40201 gggggtttga atccctccgg agtcactaaa tttgatatat aatttcaatt atttaaaata
    40261 aatttttata atcttatttc attaataaaa aaaaatcaat tcaacttttt gcttttaata
    40321 aatttgttac acactctgta ttgtttacat tatttaaata cataaataaa gaccaaagac
    40381 tttaatatac atataaatac attgttatca gttgttaatt taatcttgtt ttttagaatt
    40441 tgtaaataat attatgtata tttgtacaaa aaatagagat atgggtagag caatgataat
    40501 aggagcgggt ggagtagcta ctgctgctgc ctgcaaaatg acagaagcgc cagaagtatt
    40561 cgaagaagta atgatagcaa gtagaactaa agaaaaatgc gataaaataa taaaacaaat
    40621 agacaaaacc aactattata gtgctcaaat agatgcaatg aatataaatg aattagccgc
    40681 tcttataaaa gatttcaaac cggatattgt tttaaattta gcattacctt accaaaattt
    40741 agctataatg gaagcttgtt tacagacagg agtcagctat ttagatactg ccaactatga
    40801 gtcaccagac aatccacatt ttgattacat aaaacaatgg tcatattttg ataaatataa
    40861 atctgctggt atcacaggta tacttggttg tggttttgat ccaggggtaa ctcaagtttt
    40921 tacagcttat gcagctaaac attatttcga tgaaatacat tacttagaca tcgtagactg
    40981 caacgcagga gatcatggta aatattttgc tacaaatttt aatccagaaa taaatattcg
    41041 tgaagtaact gctaatggac gctattatga agatggcaag tggatagaaa ccaaaccatt
    41101 agaaatacat cggcctatag aatatcccga aataggagta atggagagct accttctatt
    41161 tcatgaagaa ttagagtcct tggttaaaaa ttttcctaca ataaaaagag ctcgcttttg
    41221 gatgactttt tctgaacgtt atctaacggc tttagaagta ttacaagaag taggtatgac
    41281 ctcgataaaa cctattaatt atgaaggcaa agaaatagtt ccattacaat ttttaaaagc
    41341 tgtattgcca gatcctagta ctttgggaga aaactataaa ggtaaagcat gtataggtgt
    41401 gcaactaaaa ggaatctatc aaggtaaaga aagaacatat tttatatgga gcatatttga
    41461 tcatgaaaaa gcatataaag aaactggtac tcaagctgta gcttattcta ctggggttcc
    41521 agctatggta gggactatgc tctatctaaa agaaatttgg aaaaaacctg gtgttttcaa
    41581 tgtagaagag tttgacccag atcctttttt agaaactatg cctatctatg gtattgactg
    41641 gtatgaagct gtagatatag ttttgcctat tgataaatga aaatgtccag aaaatatttt
    41701 acagagatcc catctccatg ctttgttcat gaagaaaata aacttttaaa taatttgaag
    41761 atacttgatt atgttagaaa aaaagcccaa gtgcaaatac ttgtggctct taagggttat
    41821 gccttttggt attattttcc attaattaaa aaatatcttg atggcgcatc agctagttct
    41881 ctattcgaag ctaaactaat aaatgaagag atgggagtaa aagctcatac atattgtcca
    41941 gcatatttag atgaagaatt tgatgagatt gccaagctat ctaaacactt aacgtttaac
    42001 tccatcgcac aatgggaaaa atataataag cgtataccta ctggtacatc ctgtggctta
    42061 agaataaatc ctgaatatag tgagatagaa gtggctcttt atgatccagc tattcgtggt
    42121 agtagactcg ggatagtgcg agctatgatg ccagaaaatt tacctttagg aatagaaggg
    42181 ttattatttc atgtcatgtg cgaacaagat tcttatgtgc tagagaatgt tttagaacaa
    42241 gtacaaacta aatttcatca ttatttgtta caagctaaat ggttgaatat gggtggagga
    42301 cattggataa cagatgacaa ttataatgta gaacatctta ttgcactttt aaataatttt
    42361 catatacgat atccacattt aataataatt ttagaaccag gtgaagctat aggtttagat
    42421 agtggatttt tattaacaac agtacaagat atagtagaaa ataatggaat aaaaactgcg
    42481 attttagacg tttcattttc agcgcatatg cctgatacat tagagatgcc atataaacca
    42541 atagtgagag atgcatccat agatcctgct aataagccct attcatatag gcttggtggt
    42601 attacttgtt tagctggcga ttttatggat gaatatggtt ttgatgaacc actgaaatgt
    42661 ggtgatcagt tgatcataga tgatatgatt aattatacta tggtaaaaac tactctattc
    42721 aatggagtaa aacatcctca tatcggcatt ttagatattg ataataaatt taagctatta
    42781 cgtagtatac catacgaaca ttataagtat agattgtcat agtcgtgttt tataaagtaa
    42841 aaatacatat ttgatgaaat gataatatca taaggtgatt tattactttt ttgcattttt
    42901 tcgtaccaag acaaaataaa ttaaaatgtc aaaatactaa agagagtgaa aaacactgaa
    42961 taaagaaaaa attattaggt ttagttattg gtttttaatc ttgaattttt atttttgcac
    43021 agtttatttt agtgacaata ttatctgtcc agtatccagc tctatatctg ggctatcatc
    43081 tattatgtta ttatcaataa tattttcggg aaaaatactt tttagccatg cttcatcggg
    43141 ttttatcaca ttaataaact ctatattttt tattttatta tttttaattt ttttaccttt
    43201 tgctttcaag gaattgatag gaaatttttt atctagatca ataatttctt tagttttatt
    43261 actgtaattt atttgtaagt tacctagcca ttcttttgta aaaaataata tttcacaatc
    43321 atcattttct ggtaaaaaat aaacattttt atctatgatc tcatcgagaa taaatctttt
    43381 aatatgttga gatttttgat cattatttcg ataaataaca gtgtagagag tacgaggatc
    43441 aaatttctct acaagcaagt agttagaatc tataaataaa ctctcattag gtatagcgat
    43501 gcggtaatat ccatttttat atataataat cagatagtca tcatcaaaaa actcacctaa
    43561 ttttacacct ttaccatctt tattaatacg tagagtctca ttatcaaagt aataagaaat
    43621 accttctagg gttttgatac cttcttgttt aatagaaata tggcgtatca attttttggt
    43681 aactacataa ccaacagtag aacgattttt tataggtata tcggcaaaat taaaatcaat
    43741 ggttaaattt ttcattctag gacgtggcac tagatgtata gtaagtactt cggcttcacc
    43801 attttgatta gctttgaaat acaaaacttt agaatttgga ttaccacgag tataaaaata
    43861 atctttgtcg cgatgtacac tttttacaaa aaaacgctta gcatacacat taccattagc
    43921 gccatcgcga tacaatacat tataagtagt tctagtatca ttaggatcat atatagctac
    43981 atgcaaaata ttttcaccta caaaaacttt atcatctact ttgataactc taaaggtgcc
    44041 atcttctttg aaaactatta aattgctgag tgtagagcaa tcaaaaagat actcatcttt
    44101 ttttagtgat gtacctacga aaccttctgt gcgattaacg tagagacgtt gattaggtgc
    44161 tgctacttta tctttttcta tactactaaa agttctgatt tttgtacgac gtggaaaatt
    44221 tttaccataa gtatttttta aatatttgaa gtgttctata gtataagaag ttagattatt
    44281 aagatttatg tttacttgtt ctaattgatt ttgaagtttt acgattatct catcagcttt
    44341 gaatgcatca tatttggata tgcgttttat tttgatttca gtgagcttaa taatatcatc
    44401 gcgagtgata tcacgataaa attgtttttt aaaaggttct agtcctttat ctatggtatt
    44461 aataacatct tcccaggttt cacactcttc tatgttttga taaatatgat tctcgataaa
    44521 gattttttct agagaagcga atagcaattt ttctaatagt tcatgctttt ctacttctaa
    44581 ttcttcttta ataaagccta caatattatc agtagtttct ttcaaaagtt cggtaataga
    44641 tagaaataca ggagtattat catctattac aacgcaatta gtagatatct ttacttcgca
    44701 tttagtaaaa gcatatagag catctatggt ttgatcaggt gtaacgtctg gagacaaaat
    44761 caatgtaatc tctacttcag aagcagtatt atcttcgatt tttttaattt taagatgacc
    44821 tttattagta gcatctatga tagagtctat tagttgtcct gtagttacgc cgtagggaat
    44881 ttcacgaatc acgatagttt tattatccac tatttctatt cttgcgcgtg agattacctg
    44941 cccttttttt tgaccatcat tataatttgt aacatctatc aggccaccag acaaaaaatc
    45001 agggtataaa gtgaatgact catcttttag atatgaaatg caagcatcta atacttcatt
    45061 aaaattatgt ggtagaatgc gtacttgcaa gctaacagct attccttcta taccttgtat
    45121 gagtaatagg ggaaatttaa caggtaaata gatgggctct ttatttctac catcataaga
    45181 aattttccaa ggtgttattt tatgattaaa tattgtctgc aaggctaatt tcgaaagacg
    45241 agcctcgata taacgaggag cagctgctgt atcaccagtt agaatatttc cccaattacc
    45301 ttgtgtatct attaataact ctttttgtcc taattgcaca agagcatcac ctatactagc
    45361 atcaccatga gggtgatatt tcatagtatt tcctatgatg ttagctactt tattatacct
    45421 accatcttct aattcattca ttgaatgcag aatgcgacgt tgtacaggct ttaagccatc
    45481 gcgtatatgt ggtatagctc tctctaatat tacataactg gcataatcta aataccagtt
    45541 ttcatacatt ccagatatag tcttcagtga tgtctttcct tttgactgtt caatgttttt
    45601 ttctttttca gtttccatat tcataacaaa attacttcat ttattagtaa tgacaaatag
    45661 atattgataa aataatttaa aattataaat ttttattgat taaaactttt tgaataatac
    45721 aattttacat tattataatt ggataaaaaa ctttttgtat ttcacattat agaaattgtt
    45781 agtgcatttt ataaagaatt atttaataaa atgtctaatt tcatatagca attgcaacaa
    45841 aaatactcct tctaaaaaaa ttatttatta tgcaaacaaa aatttttata taaatttgct
    45901 aaaaaaaaat tatattatga aatatttatt tctttgtcaa ggtaatgttt gcagatctaa
    45961 tatggctgaa gagattttaa aagaaagatt gatgcagata aatagcgatt ctctcgtaga
    46021 ttctgctggt ttcgaaccat acttaatagg tgaagaaaca gacaaacgtg cacaaaatgc
    46081 tttattaaca aaaggatata agccatctac tcatagagct cgtttatttc aaaaaactga
    46141 ttttgatgat tttgataaaa tttatgtaat ggatgctaat atatatagat tagttaaaga
    46201 aatggctcga aataataaag atattgaaaa aatagattat ttaatgaatg aagcctatcc
    46261 cgaaaaaaat ataccaattg ttgatccttc ttttagtgat aatcttaatg attttataaa
    46321 tacaatagaa atgatagaaa atgcaataga tgcaatcata gaaaaatatg aaaaataatt
    46381 agaaatttat tattttttat agataatcga tatttgttaa tctatttttt tataaatatt
    46441 tttttaaata aattttatat ttaaataaat attatataat tttgtgatat aaaaatatta
    46501 acagatatga aaaaaattgt attattaatc attttaatca ttagtacatc tattatcttt
    46561 gggcaaaaca atcaaaatga tgaacgaatt gtgccaattg aacaaaaatt tcaagaagct
    46621 aattgggtaa ctactattac tgatctagat tttagtaaca ttattttgct atccagtttt
    46681 gattggcaag ctattaaaca tgaggattca atcagatata atcaaaaatt acctactcgc
    46741 ataggtatgt ctatagccat agatgtagat ttattaaatg atattatccc gatagaagaa
    46801 gatggatatt acatttatag gtataaagtt tttgctgaag gagccaaaaa agtaggattt
    46861 gtttttgatg atttcttttt gcaaccaggt gatgctattt atataataaa tgaagatgga
    46921 caatttattg gtccagttac agatgtagaa aacaaagaat ttaggatgta ttcgactgtg
    46981 ccattaaatg gagaagaaat aactttatta tatgtaaaa
//
LOCUS       DAOPRN010000008        46822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_574_length_46822_cov_98.798533, whole genome shotgun sequence.
ACCESSION   DAOPRN010000008 DAOPRN010000000
VERSION     DAOPRN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 46822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46822
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_574_length_46822_cov_98.798533"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..3085)
                     /gene="uvrA"
                     /locus_tag="PKI65_01800"
     CDS             complement(239..3085)
                     /gene="uvrA"
                     /locus_tag="PKI65_01800"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOB77206.1"
                     /translation="MSNDYLVIEGARVHNLKNVSIEIPLHKLIVFTGLSGSGKSSMAF
                     DTIYAEGQRRYMETFGAYARQFVGVFDRPDVDNITGIPPVVAIEQKTISQNPRSTVGT
                     VTEIYDYLRLLYAKVSIAYSYLTGKPMLQLSIDEIIKKILEKFKGLHIHILAPLVQAR
                     KGHYRELFDRYAKLGFEQMRVDGKLVTIEPGIKLERYQTHDIELWIDELDVSHNKIRR
                     LKNSIELALKYGNGNLIILHDEKATYYSKNLMCEDSGISYPMPEPNTFSFNSPYGACP
                     TCRGLGYVNEIDIDKIFPNKRKSLREGGIAPFGKYKNNWTFKQLEMILKKYDLTLDDP
                     IEVFPDELIQTLLYGTDEIIHMQYDNIGISSNYQLSYDGIINIVNKLAEADNQMMRKW
                     ANTFQVVNICPTCEGTRLKKESLYFKIDNKNIAEIANMELSDLIYWIDKLPKILTEKQ
                     NLIAKELLKELKTRVNFLLNVGLSYLTLNRPTYSLSGGEAQRIRLATQIGTGLTGVLY
                     ILDEPSIGLHPRDNNQLIKSLIKLKDLDNSIIVVEHDRDMMLSADQIVDFGPGAGERG
                     GKVIAQGTPKELMQCNTLTCQYLSGKKKFKLKLDHRTGNGKNLTIIGANGHNLKNITV
                     NFPLNKLIGITGVSGSGKSSLITETLYPAVFNALNKSFRNCLPYEKLEGLENIDKVII
                     IDQSPIGRTPRSNPATYIGIFDYIREIFANQPESKMRGYNKSRFSFNVGNGRCSTCKG
                     TGYLVYEMKLMDNVYTTCPSCNGKRYNRETLEIRYRGKNISEVLEMTFQQAYNFFEGN
                     SRLQKSIKIINDIGMGYIRLGQSSVTLSGGEAQRVKIATELIKRSTGKTLYILDEPTT
                     GLHFEDISNLLKTLDRLVDMGNTVIVIEHNLEVITHCDHIIDLGPEGGNLGGYVIYNG
                     TLQDMLSIDQGYTAPFIRKYVAEQLGQETLELIR"
     gene            3324..3491
                     /locus_tag="PKI65_01805"
     CDS             3324..3491
                     /locus_tag="PKI65_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77207.1"
                     /translation="MNAIHTPIGRYGGALSNVRTDNLTVLIIKAVLARNLHVDTNGID
                     EVVFCCSRFSR"
     gene            3496..3873
                     /locus_tag="PKI65_01810"
     CDS             3496..3873
                     /locus_tag="PKI65_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOB77208.1"
                     /translation="MKTFALDRLLNLKITDISFCEFKDFDANEYFKYCFGIVRPENAV
                     VEEVKLSFTPKQGKYIKSLPLHATQKIIIDNDDEFVITLSIFITYDFIQEILQYGDEV
                     KVIKPDSLVKTIKQIHENALRRY"
     assembly_gap    4069..4168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4294..5388
                     /gene="ricT"
                     /locus_tag="PKI65_01815"
     CDS             4294..5388
                     /gene="ricT"
                     /locus_tag="PKI65_01815"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOB77209.1"
                     /translation="MDSDTIKSLNARGLIAINIDSKTELLFTNRWQKLTSYKDAKHDK
                     DFYIAFPEAIEYVEVQFKNDRKQFYRNTTGMPLEIGSLVAVEANPGHDIGVVCLTGRE
                     VIQQMKRKKSKNNNGELLNLYRIAKENDIDKFLDAIKNEEKVIKQTKKIIKEKGLEMK
                     LIDVEYQGDGTKATFYYIADERVDFRELIKTLASKFRIRVEMRQIGARQETAKLGWIG
                     PCGRELCCSTYKCVFSSVSITAASIQQLTLNPQRLAGMCTKLKCCLAYELPLYEKFIT
                     SLPPTDIPLITKEAKATYVKADIFKNVMYYSYENSDLLFGLPISAVIQIQELNKKGEI
                     PEKLEEFAVISEYTVEESVSQEDLSKLEDE"
     gene            5381..5857
                     /locus_tag="PKI65_01820"
     CDS             5381..5857
                     /locus_tag="PKI65_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOB77210.1"
                     /translation="MNNNFFILIFIVILLLSSCKQDKYYDIQSFDDEVWVYTEWKEFA
                     IPIPDTINLYNFHLLLRTSQEYPYTNLYLFVQNIPPTGSIYEDTLLLILYDENGKPIG
                     KKSNKNYIYDFLIRKNYIFPSTGVYTLLVTHGMRDSILPGIIEIGFKFEKVNKNER"
     gene            5847..8237
                     /locus_tag="PKI65_01825"
     CDS             5847..8237
                     /locus_tag="PKI65_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOB77211.1"
                     /translation="MKDNFFSIFKTEDENFKKFLKNLWIIFFCILGFIFIFFIGVYYG
                     LLGPIPSFEQLENPLDKFSSELISVDDNVIGKYYYEENRSWVSYNEISPYVIQALIAT
                     EDKRFYKHSGIDFKGLARVFFKTIIGQQKGSGGGSTITQQLAKNLFKRPDNINKIKLI
                     GIKFREWMTAIKLERNYSKDEIIAMYLNTVPFGYQLYGVKSASLYYFGKNPNEIDIDE
                     AAILIGMLKATTRFNPILNPENAKNRRNIVLKQMLKNQFINKEQYDSLKVKEITKPET
                     SITTKTDDLAPYFREYLRLYMTAKEPDHKKYKNKADYKKAKELWDNDPLYGWCNKNTK
                     PDGKPYNLYSDGLRIYTTIDSRIQRYAEAAVREHLSQDLQKKFFQEAKRSKYAPFVGA
                     TSQAEVDKLMDMAIKRTDRYLSMVRDGYSEKEINKAFHTPTEMRVFSWQGIKDTVMTP
                     YDSIIYHKYFLRASFVAIEPQTGYVRAYVGGPAYSFFKYDNAAVGKRQVGSTFKPFVY
                     ASAFINDPTLTPCTKVPLIPVTFDMPDGSQWTPRNSGNVQEGTMIELSKALALSINWI
                     SAFLIKEKTTPEAVVELAKSMGITDTIPPVPSICLGTAELKLIELLSALSTFANSGVA
                     QKPIMVTRIEDRFGNIISVFPYGVTHEVMDEFTAYKMISLMKGVVDYGTGSRIRYIYK
                     IPYPVAGKTGTTDNNSDGWFIGITPKLAAGVWVGGEDMQIHFRSMALGQGASMALPIW
                     GLFMQKVYADDSLNLYKGDFDRPAGMSYEMLCNPSTTDADGSSMPFIMPKKKKTWN"
     gene            8239..9720
                     /gene="ispG"
                     /locus_tag="PKI65_01830"
     CDS             8239..9720
                     /gene="ispG"
                     /locus_tag="PKI65_01830"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOB77212.1"
                     /translation="MRRKTLQIPIGNILIGSEYPIIIQSMTNTLTSDIQSTVNQCIRL
                     FDADAKLVRITARNLKEAECLKLIKNDLINMGYELPIAADIHFNPHLAKVAANYVEKI
                     RINPGNFVNLFPNNKSYTNEQYKLEVDAIKNEIEKLLPILVANKVAVRIGVNKGSLSQ
                     RLIDKFGNTTEALVESAIENVKIFNKLNFNNIVISIKTSSVYETIAANRLLVSKLDKL
                     GLYYPIHLGVTEAGIDKEGIIKSSVAIGNLLLDGIGDTIRVSLTGDPVKEISVAETII
                     KTYQKFLPLPPMPTYSYTLENAPKIFTINDLTTEHLDLNNLFIDLPKNRPFDFIANKL
                     KTVEQVNIKIDIDKFDLYELSIILGRGWIDGWLKDVYLVCDNLKNEKVNDVYQILQVT
                     GKKKLLTEVISCPSCGRTMFDIESITRQVKKRFSIYPGLTLAVMGCIVNGPGEMREAQ
                     AGIVGNGINKADIYVFGRVVAKNIPVDKVVDEFEKQLKIHNLI"
     gene            9939..10313
                     /locus_tag="PKI65_01835"
     CDS             9939..10313
                     /locus_tag="PKI65_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77213.1"
                     /translation="MKNNFKQTFINLLSLFMAVFMILLIVNNIVFLHVHKLPNGEIIV
                     HAHPYNKSNDTEPIKNHTHSNREYLVLDQLKNIFVITFIFFSFLFFSNKILIFKNKIV
                     YHGFDHDKFYKNKAPPYFILNY"
     gene            10410..12788
                     /locus_tag="PKI65_01840"
     CDS             10410..12788
                     /locus_tag="PKI65_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOB77214.1"
                     /translation="MKNKKIVFILCIMLCTLNIYSQKKETDANIIGHVMCDGEHLPFA
                     SIYIKGTNIGTTSDETGHYKIINIPIGTHILVARSVGYKSQEIEIDVCIGETKEINFE
                     LEKDVLNLSEVVITGERKMIYKKKSSIIVNTLSPKLFDLTQSTSMGENLNYCPGLRME
                     NNCQNCGSSQVRMNGMDGPYSQILINGRPIFSSLASVYGLELIPTNMIDRIEIVRGGG
                     SALYGSNAIAGTINIILKDPSINSYEFGISQGIIGIGIDSTGGIANDFSIKFNTSIVS
                     TNNKTGMTLYGFYRNRNPFDANNDDFSELSLLKNLSMGTRLYHKIGTKSKLSTDFFII
                     SEKRRGGDKFDYPVHESNITESAEHFITTGALNYERYFRKNDLLSIYLSAQKVDRDSY
                     YGANQSLNSYGKTQDVSLVVGAQYNVYFDKSNLIFGVENNGSWLKDKKLGYPDIDNAM
                     IINDIIINIPHVNNTILTNQTSNIIAGFAQYEYNWERMKIAIGLRLDKYNIKDKNKSK
                     NNISGNILNPRINFKYDFNSFCQGRISYSQGYRAPQIFDEDLHVETSGARQIIHINSP
                     NLKIETSRSYMMSLDFNKNFSNIYINFLIEGFFTKLNNPFTNVFGLPDSNGIVIYTRI
                     NADDGASVMGVNIEFNIIPFEKLSINTGFTLQSSKYDVPQEFNEKRFMRTPNNYGYII
                     LDYKLTKKFGIALSGIYTGKMLVPYFGNQIADPMAGELRNTQNFFDFGLKTWYNVKIN
                     GATLQLFIGIKNIFNSYQKDFDVGLDRDPGYIYGPAYPRTINFGIKIGNAIK"
     gene            complement(13106..14338)
                     /locus_tag="PKI65_01845"
     CDS             complement(13106..14338)
                     /locus_tag="PKI65_01845"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOB77215.1"
                     /translation="MKIKHYIIFLLLFIIIFISCKTSKISTSYPFYEFYQATPYNIRS
                     NAFFYHLSDSISILNIEIDTTNLHFVNLPTPDSIFYKIARIECKIDLYDSLDKILKSS
                     IFKISIADTISRWDTTIKLQINHPFKGYCRLFIKSVDFGKNYTVKYLNIDKTSPYSAQ
                     WFYTNLQDKHFLKISDTFKIYNSAFDIDSLVFSVYKFPPVAAPPFVTELNLKDIEIEK
                     IKDHSISASQSWVATSDLLNTLINVSIDTFSTGIFFIVVPAHFPEIIYPYELVPPLRY
                     ITTDTEFNSMLKSEYPKKTVDSFWLAQIGEAKRAAEVIKKYYTRVTDADKWFTDIQPG
                     WQTDKGLIYIVQGPPNYIYRLYDTEIWLYGSKDEMYAKSYYFYRTYIPNTKMYTWKLY
                     RNNEYKPFWFYNVNLWRK"
     gene            complement(14335..14961)
                     /gene="recR"
                     /locus_tag="PKI65_01850"
     CDS             complement(14335..14961)
                     /gene="recR"
                     /locus_tag="PKI65_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOB77216.1"
                     /translation="MDLENLSKTMANAINELAKLPGIGKRTATRLVMFLIRQDPQYIK
                     KLSESIALLSTNLFLCRQCHNVSDAEICNICSDMARDTTILCVVQNIQDLIAIERSRV
                     YNGIYHVLNGLISPIDGITPDKIFVNDIPERLRNNDINEIIFALPATVEGDTTAYYIF
                     NMLKDVNSLYFSAIARGISIGGDIEYIDELTLGKAIKNRIDFSKAIKK"
     gene            complement(15065..15979)
                     /gene="xerD"
                     /locus_tag="PKI65_01855"
     CDS             complement(15065..15979)
                     /gene="xerD"
                     /locus_tag="PKI65_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004361053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOB77217.1"
                     /translation="MFFKKMNEEYLKSFHTYLMLDKHNSKNTIDAYLRDVKKLMNYFN
                     NINKSFLDANLKDLEGFVSFLSELELESSSLARIISGIRSFYNFLLEEEIIKENPAER
                     LKLPKLTRKLPEVLSVEEIEQIIAVIDLSKFEGHRNKAIIEVLYGCGLRVSELVNLKM
                     IDYFEEETMIRVIGKGDKERYVPIGSKAKKALHYYIEYSRPHYPVITQYQQYIFLNRR
                     GKKLSREMIFIIVKDCAAKAGISKNVHPHTFRHSFATHMIENGADLFAIQEMLGHSSI
                     TTTEIYTHISMSYLRDVIESCHPRNKIS"
     gene            complement(15981..17318)
                     /gene="purB"
                     /locus_tag="PKI65_01860"
     CDS             complement(15981..17318)
                     /gene="purB"
                     /locus_tag="PKI65_01860"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOB77218.1"
                     /translation="MTNLLQISPIDGRYCEQTKILENYFSEFALIKYRLNVEIKYFIA
                     LCKIPLPELENFLNEERERWLLSIYESFSIQAANEIKAIENEIRHDVKAVENWLRVQF
                     DKKGFEKEKEFIHFGLTSQDINNTAFPMMMRDAWFEVLYPSIEEILNILNDYSNKWWQ
                     IIMPARTHGQLASPTRLGKEIAVFIERLNNQLHLINFIPFAGKFGGAVGNFNAHIVAY
                     PDIDWVKFSNEFLSSLGLIRLQNTTQIDHYDYSAAFFDALKRINNILLDLSRDFWLYI
                     MLDYFHLKIDKREVGSSTMPHKVNPIDFENAEGNLGIANAQLEHLSNKLPISRLQRDL
                     SDSTVLRNIGVPYAHSLIAYNSLKFGLNKLDVNKSKIDEDLDNHWEIIAEGIQTILRR
                     YGYPNPYEALKQFTRGKSGLKKHDFVDFINQLDVNDEIKEKLLNLSPHNYYGI"
     gene            complement(17506..19749)
                     /locus_tag="PKI65_01865"
     CDS             complement(17506..19749)
                     /locus_tag="PKI65_01865"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOB77219.1"
                     /translation="MKSTIIFSFFMILACLIAKTQNNQNIYLYPADDVLLKLKVIEDP
                     HLIVDYMIYEDNFKALFNKDFSFEKTDTLIDGKRIIPVVFHIIHTYGSENINKAQILD
                     GLEKINIDFNKQNADTVNTYDLFKSRAANCNIEFRLAQKDPNGNCTDGIVRHYTPETN
                     YAYFNTMKKYVWDPTKYMNIFVVNFIYPEGLALPEGALIGGMSPFPPDNPLSQALTGG
                     DTDIDGVLIRHDCIGGIGTAENFADMPLNMVNRYFTHESGHYFNLYHTFQNLILGLIP
                     TSSGCPSMLATTGDEVDDTPPVDQATQNTSISCFTPGSRNTCTEDPDEPDMIENYMDY
                     QWGYCNNIFTLGQYQRIDATLNGYRRNLWSVENLQATGVLEEDSHECAPIADFFSNTQ
                     YVCAGSEISFFNASFNGTATTFNWTFQGGNPDNSTLENPTIIYNSPGVYSVTFQVNNA
                     QGTNTLTKANYIHVYSSTTNNTAPISESFEYTPINDFIVINDTGSVWQIKTGTSYSGN
                     KCIYLANFIDNVAGSKDEFITPAYNLTSLPSGHAKVSFKVAYSGKIIPSTILTPADTI
                     YDKLTVYISNDCGKTWQSRLVKSGQNLITTAPIESEFTPSSTTQWAEFSFIIQSSYVQ
                     NYDNLRLKFSFYSNGGNNIYIDDINILSMSDINTQVLAGNQIQLYPNPLNENTQIYLE
                     LNNSYKVSLQIIDINGNLVHNILNDYMNNGNYSIPLNNLNNLSNGIYYIRVRLDNNET
                     FLPLLML"
     gene            complement(19978..20955)
                     /locus_tag="PKI65_01870"
     CDS             complement(19978..20955)
                     /locus_tag="PKI65_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOB77220.1"
                     /translation="MKQLAKQLIIILITTTISINLFGWGFFAHPRINEHAIYCLPPEM
                     VGFYKRHKDYISQHAVDPDKRSYVDPIEAARHYMDINHYGQYPFDMVPKTWNDAVKKY
                     TEDTLYAYGILPYHLIKMYYRLVDAFKEGDAGKILYLSANIGHYVADAHVPLHCNEYY
                     DGRTIEEKGIHALWESRAVEVLYDNFNLFVGRAKYISKPVDEFWNWIKLGYLQSDTVL
                     SVYAYMLKHYTPSQIYTMEDRNNILIKQYSREFVQEFNALLHNMIERNFRRAIHNVAS
                     FWYTAWVAAGQPNLDILDAKDFIAKQKKEEEELDYLWKHGKPLGRPNPE"
     gene            complement(21036..21758)
                     /locus_tag="PKI65_01875"
     CDS             complement(21036..21758)
                     /locus_tag="PKI65_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOB77221.1"
                     /translation="MLSVEVVLSPLFLDEPKVITKPPFNSIVIDVLRATTSICTAFMN
                     GAKEIIPLQNEAKTYEYKNKGYLLAAERDGIKLPHADLGNSADEFSINRVRGKSIAYF
                     TTNGTKTILRAYSIAEVYLGSFLNIRALADHLIQENKPIVLISSGWKDFPNLEDTICA
                     GAFVQYLLLHGYQCIEDSAYIALDLWEMSNNNLEAYIKKASHYKRLHDKLNLAHIVPY
                     SLQLNITNVIPFFDGEKIIDLS"
     gene            complement(21915..23159)
                     /locus_tag="PKI65_01880"
     CDS             complement(21915..23159)
                     /locus_tag="PKI65_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77222.1"
                     /translation="MKRTVSIALIISLSAIFIACKNSECIYCDKIHRGKLIDSIKLAN
                     TNYKYSAYIPERYKHKKLPTFIFFDPHGHPELAMKKYKSIADSLGVIFIGYTLSSNET
                     PANFSLSYFEIFIRDINRKLPVDTAKLFFVGFSGGSRVATIVQEQFQPAKGLILTGAS
                     AYNPEFYSKNMIPIVHIVGIKDFNFSEFFHLFTQYANSPSIAIIFFDGKHHWPPQNIV
                     DLAIKMLIFDKTKMKKQPNYSLDSIPEFFLYPTLLSMKKFNFLTSEDQLIKSYEIFSK
                     KTMPILFDIFEKEWSEQQKIINFFNFADDKKLIKYIDSLRNISLNSNHIIDEMNYALA
                     QRLLSFIGIISFSYSANLFQQNNPAMQSILNIYEYIEPDNKDMLYFQVKWHLKNNDSV
                     KANFYLQKADSLYPNFNVFIKN"
     gene            23219..23785
                     /locus_tag="PKI65_01885"
     CDS             23219..23785
                     /locus_tag="PKI65_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOB77223.1"
                     /translation="MGKINMPLSSLLLKLLGWKVETEEDVTKYKKAVLIMAPHTSIYD
                     FIIGWLALKKLNVKAKFLIKKEFFFFPLSIILKSLGAIPVDRGDPKNLPRYLVKLINK
                     SSEMWLIITPEGTRKPVKNWKRGFYYIADSAQIPIIPSYLDYQKKIAHVLPAFYPTGN
                     YYSDIQPFIDILANVNARNKNNFVLPKN"
     gene            23792..24862
                     /locus_tag="PKI65_01890"
     CDS             23792..24862
                     /locus_tag="PKI65_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HOB77224.1"
                     /translation="MQETNFLALFRYPREWIEDLINEINNAKNWIYIEIYRFVNDPTG
                     IRVRDALVEKAKENIKVYVLVDSFGAKSDTNFFKPLIEAGGQVRFFTPIPISLAIFSE
                     AHRRNHRKIIVIDQISYLGSANISNYSLDWRESIFKFDDPDLARRLVYFIKLYFKHYK
                     TYTFNQKKFHRIHNVNGMELIREIPSIWKQRIRKKLNWLLDNSQKSIKLATPYFLPMG
                     STIDALIEAAIRGVDTQIYLPFRSDVSSMDVLRNYYLGKLWRAGIRIFFYLPTNLHAK
                     LLLLDNEQYMIGSCNYDYRSYLYMHEINMVAKDPVVVNELEKYFSELKQDSIEFDYDR
                     WRERPVLDKIIEKILIPFRFLF"
     gene            25068..26135
                     /locus_tag="PKI65_01895"
     CDS             25068..26135
                     /locus_tag="PKI65_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77225.1"
                     /translation="MKYFCLFIFIFLISFNLFSQLGDSIDKSKPILHVFGNFQYDATK
                     GANKKFGFGIGRAYLGYQYQFNDNFIGQVIVEADKPTTIDKIEVKDSLDNLLYVIYQE
                     RIGSNYTMQLKIASLQWKPNDKIKIQIGCIELNNYNTQDKFWSYKYISETFQDKYFKL
                     PSADLGIISYFSINDYIDLDLALTNGEGNRFDQDLFGDLQVASGIDIKPIESLHIRFY
                     YDYSKSKDSLRPAERQLFSIFTGYQYDNRFKIAAEYNYRKNNIFINNNDLYGFSIYSI
                     YNFYNKFSFIARFDKLYVKNIYIDNESWYSHYPGNGYVIGFNYNPAQKINFSINYQLW
                     QPKDKNIFLQHNIFFNFEFKI"
     gene            complement(26144..26521)
                     /locus_tag="PKI65_01900"
     CDS             complement(26144..26521)
                     /locus_tag="PKI65_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOB77226.1"
                     /translation="MRLDKFLWYVRLFKTRSLASEQCKKSRIKVNNQIAKPSKEVHEG
                     DIVEIKCTYYVKTIKITALPHQRIGPKIVNQFIEDLTPDEEYQKSDMIRYDLSPYKPK
                     WKGRPTKKDRRDIDNFLNDILNN"
     gene            complement(26525..27826)
                     /gene="der"
                     /locus_tag="PKI65_01905"
     CDS             complement(26525..27826)
                     /gene="der"
                     /locus_tag="PKI65_01905"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOB77227.1"
                     /translation="MRGIVAIVGRPNVGKSTLFNRLIGSRLAIEDPTSGVTRDRIYGK
                     CYWNGVEFTVIDTGGVVIKSDDIFQQEIKKQVDLAIDEADLILFVVDTHDGITPLDID
                     IANLLRPQRKPVLLIANKTDSSSIYHYHTEFYQLGFDKIFPISAKNGLGTGEMLDVVV
                     ELLPEMKDDELDYPRIAIVGKPNVGKSTILNTLLGSERSIVTPIAGTTRDTIYEVYNK
                     FDHKFYLVDTAGIRKKSKINDNIEFYSITRSIRAIESADVCILVLDATEGIGAQDLNI
                     LSIINDNKKSVVLAVNKWDLIDKDTKTANEYRKMLMEKLAPQDHIPIIFTSAIKRQRL
                     LQVLEVVKKVYDERKKRIPTSTLNKIMLNEFEKNPPPLYKGKKVKIKYCTQLPTPYPS
                     FVFFCNSPRYIKEPYKRFTENKIRQHFGFDGNPVIIYFREK"
     gene            complement(28197..29972)
                     /locus_tag="PKI65_01910"
     CDS             complement(28197..29972)
                     /locus_tag="PKI65_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOB77228.1"
                     /translation="MKIAFIFFTLIISSLLSGQNNKIFTLPIDNEPDKSLSYEQVMKL
                     SHELAKQYSDNIHFTYLGLSEQNDTIAALIIFKDKANNPTEIAKQNKVTLMIQAGIHA
                     GEPDGIDGGFMLIRSLLSNPRYMNLLNNVTIIFIPAFNVDGLKLLSPYHRINQNGPEL
                     QGWRANAHNLNLNRDYLKASSIEMKNWLKFYYYWNPDFFIDCHTTDGADYQYVMTFDM
                     QLPSFVSTDFNNFFEHNFITYFIDEMDNNGFLSTQYVAFRDWFSPESGLTASIYSPIL
                     SHGYPLLNNKPALLLETHMLKSYKQRVMATHQALLIVLEWLSNNKNLLKNLLLNEDNY
                     SLSMAKPNKTFPITYKLTEHQNKKILKGYEYHKAISPVTGISYYTYDNTKPMEVALDF
                     WDSIEVKTNVVLPYGYLLGPEWEHLIEILELHQVTSYTLSEISKIKVQYYYLTKLKFD
                     NAIFEGTFSPIFSYQLRDTFIDLPRGSYFIPINQKAWKSLVYLLEPESTVGFMQMGYF
                     NSMFERKEYGEIYVLDTLAQNMLNDKNIADAFREKKLKEPEFANDPWAQLMWFFDNSP
                     WKDQRMRTIPVFKVMDKENYQKVNE"
     gene            complement(30467..31066)
                     /locus_tag="PKI65_01915"
     CDS             complement(30467..31066)
                     /locus_tag="PKI65_01915"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HOB77229.1"
                     /translation="MNILVHLFFAPDDDEVILGNFIADYIRGFNRLKSYTKNIQKGIK
                     LHQWIDNYSDSNSSVKKSIRRLNPLLQHYAPVAVDIIYDHFMANHWEQFATKPFLIAI
                     NEFYILLENNYNILPTKAQKAVDHMITNNWLLMYKDISGLQNVFEWMSGKTSFDSELP
                     SAVEVLLDNYSEFENDFLVFIQKMMIDFKNSEIYRYINK"
     gene            complement(31098..32981)
                     /gene="htpG"
                     /locus_tag="PKI65_01920"
     CDS             complement(31098..32981)
                     /gene="htpG"
                     /locus_tag="PKI65_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOB77230.1"
                     /translation="MRKGFIDVKSEDIFPIIKKYLYTDTDIFLRELISNAIDATAKLS
                     KLVSLRKAELELGDLTIKVEHDPKKKIIKVIDRGIGMTTEEVEKYITQIAFSGAQEFV
                     EKYLKNQEKVEDLIGHFGLGFYSAFMAADKVEIYSKSYLPETKGVHWVCEGTTEYTIE
                     EVDLPDRGTTVILHLSEDANEYNDEYNILNILKKYCRFLPYPIQFGTEKKNIGTPEKP
                     EYIDIPRIINNTQPLWKKNINELKDEDYLNFYEELYPYSLEKPLFWIHLNVDYPFTLN
                     GILYFPEIKNTIEPQRNKIQLYCNQVFVTDNLEDIVPDFLMLLHGVIDTPDIPLNVSR
                     STLQADANVRKIKNHITKRVADTLEEMFLKDRKDFENKWPALRDFVRFGIMTHQDFKQ
                     KAINFALFEDINHNFYTLNELKEKIKDTQTDKNKKIVVPYATDIDSQHSFIKPAIDKG
                     YTVILLNSILDSHFVDFLDREYEDLSFKRIDSDTLDSLIEKDKKSDVAKISEQQEQEL
                     QKLFESLLPKDLKNANISISIRNMPDESLPVVMVQPEILRRFREMAALGGMEYLEGLK
                     KEYTMIINSAHPLIEEILNYNDNNEKNKKVEQLIDLALISQNLLDGEKLTDFIKRSIE
                     IMK"
     gene            33612..36101
                     /gene="priA"
                     /locus_tag="PKI65_01925"
     CDS             33612..36101
                     /gene="priA"
                     /locus_tag="PKI65_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOB77231.1"
                     /translation="MLVNVALPLPIDKTFIYRVPSDIEHDIQWGIRVIVPFGAKKLYA
                     GVVIDILSDEQLQYIDVFTNNIDEIKYILEVLDDKPIINNEQWELYKWVADYYLANIG
                     DVLNNALPSGLKIQSETKYQLHPDIDLSSIKTEEENLIYEILLEREFITNEDIQKLLG
                     IKNIQRFIKSCIDKNIIYPTEECEEKYIPKNVKFINLSEEFLDSKDFNDKINKLERRA
                     PKQIDVILYLLQKRSQGQTKIPKAELTNKFSISAIQSLIKKGYIIETSETISRIENKF
                     ATTNEIEEIILSDIQKSAYQEIKNYINKNKHVLLYGVPGSGKTEIYSSLIQEYLDAGK
                     QVLYLLPEISLTTYLVNRLQKYFGDKILVYHSKFNLHERVEIWNKLLDLWEKDDPSCI
                     VVGPRSALFLPFYNLGLVVVDEEHDPSYKQQDLNPRYHARDMSYILANNHHANLIMGS
                     ATPSLESWYNAKIKKIGFVEIKERYSGIIPPLIEIVDMKSMMQQGKVRGHFSHPLLKA
                     IDDEVKAGRQVLLFQNRRGYSSWMECKNCGYIFPCPHCDVNLTYHKSENVLKCHYCGY
                     TIDIPTFCPKCKSNKLIFKGFGTERVETELQKYFPDYRIDRLDLDTTRRKHAFKEIIQ
                     RFERRETQILVGTQMISKGLDFSNIQLVGVLNADNLLAYPNFRVYEHALQLLMQVAGR
                     AGRREVQGRVYIQTYALNNPILPYLVNNNYEDFAENELKDRWQFKYPPYYRLIQIQLR
                     HKKEDILKIAADALTEKIRNIIDAEYVKGPVEPVISKIRDYYYRQILIKIPRDNLLKD
                     KKQKINEVVFYTLKRNKAWRGVLVDIDVDPV"
     gene            complement(36398..37987)
                     /locus_tag="PKI65_01930"
     CDS             complement(36398..37987)
                     /locus_tag="PKI65_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB77232.1"
                     /translation="MKVKNLCTTLIMVMSINLFAQKVNHTEIFKDNNAIITVNLIQDV
                     YYENYDNKNAKETLEFISDGDGTVYTFDSLSTIPTSGVEKLFSNSFIVNTSFTISNGD
                     ILKINNNDTIILSNGVTINIEGYADFTPSDTALVTRSDASANPKGIYIKGDNSRATIQ
                     NMRFEYAGIKSSSPIGIEIRNCTFTLVNTKLSSSAINLLNGNNVIENCIFISNQSSAV
                     ASGSNVSCSLSFKYNYLYDNNTNNTNRPQINMGPSGDGTIEIVGNVIIGAQRSKVGGI
                     GVANLLGVSGVNNVIISDNFVNNCRYGITTNGKMNALISNNQIIDNKYDPDPMTGGSG
                     ISIYDQNNIQNTMITGNHIEENLWGITIVSGGNVNIGKLCVDSTDSEFNPGLNVFVNN
                     GNNGILYDLYNNSPNTIYAQGNTWNVEVQDSVSIEEVIYHKNDDPNLGEVIFMYHPDN
                     VYTDDFYNSEFNYFINNNKIYFYDQIPSNINIYNINGSIVYSINNNTNNIISLENIMP
                     GIYILNILSNNKEYNSKIIIN"
     gene            complement(38168..39133)
                     /locus_tag="PKI65_01935"
     CDS             complement(38168..39133)
                     /locus_tag="PKI65_01935"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOB77233.1"
                     /translation="MSKKKHKIVVTTHKNPDGDALGSSLALSRFLKKIGHNVNTIIPD
                     AIPSFYKWMPGIDDSYIFDNDHELCDSLISDSDIFIATDFNSLTRNLKMSCSTQSIKA
                     FSICIDHHLNPPEDEWNLLISDTKVSSTSEIIYDIIDFINPSMFDKEIAECIYTGIYT
                     DTNKFFHCCDHSHPYEIMTNLIKMGVNVNKINNLIYNSYTYDRFKLLGYSIYKKLNFI
                     ENKGAAYIILTQKELQKFNYEPGYTEGIVSMPLSIEKVNISALISERKDRIKISLRSK
                     TNYDVRYLAQRYFNGGGHKNASGADFYGSLDDAEKILLKAIEELF"
     gene            39235..39654
                     /gene="ndk"
                     /locus_tag="PKI65_01940"
     CDS             39235..39654
                     /gene="ndk"
                     /locus_tag="PKI65_01940"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOB77234.1"
                     /translation="MAIQRTLAIIKPTAVQNGHTGAILHTICANGFKLRALKMMKMTK
                     TLASTFYQEHKDMEFFNELVEFMSSGPIVATVLEKENAIEDFRNLMGTTDPATSKPGT
                     LRYQYGTSIRANAIHGSENEESAKREIELLFTKKEIF"
     gene            complement(39780..40280)
                     /locus_tag="PKI65_01945"
     CDS             complement(39780..40280)
                     /locus_tag="PKI65_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOB77235.1"
                     /translation="MLDRIIKIMEYYKLTPSEFADTLEIDRSRLSHILNGRNKPSLEV
                     VMKILENFPDISSEWLLFDKGDMFKKKIFSKDQLQTGELDLIIPEKTKQKIKDDKIID
                     DLSINEQTKETVPPSTTEQTESPIADIQEDDISKAEMKPIRQKDIISIVWFFSDGTYK
                     YFYPES"
     gene            complement(40921..41823)
                     /gene="menA"
                     /locus_tag="PKI65_01950"
     CDS             complement(40921..41823)
                     /gene="menA"
                     /locus_tag="PKI65_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOB77236.1"
                     /translation="MPTIKNWISAFRLRTLFLAIATVILGSSLAWHEGMFYGKIFFLT
                     LILAVSLQILSNLANDLGDYQKGTDTTGDRKGPIRTLQSGYISVKQMKKAVGLVIIWV
                     AIIGLTLVNYSTKFIGWKSTIIMLIIGLLCILAAVFYTLSKHAYGYKGWGDFFAFLFF
                     GPVAVIGTYFLHVHSLDFQPVLPAIGMGLTSTMILNINNMRDIDNDLASGKITIAVKL
                     GLKNAKIYHLILTCSMCLCFISYSIIYASFPWHRYLYTFVFLLSFIMFFKIFHKSGKD
                     LDPYLKYTSLFSLLLVIAFALCIN"
     gene            complement(41923..42498)
                     /locus_tag="PKI65_01955"
     CDS             complement(41923..42498)
                     /locus_tag="PKI65_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOB77237.1"
                     /translation="MENENTSFIELVKRRYSVRSYSDKPVEDKKIIKCLQAVHLAPSA
                     CNAQPWKFIVVNEPNLKNQIADATSKGIVPINHFTKQAPVHVVVIMERPNISSTLGSV
                     IKDKPYNLIDIGIATAHFCLQATELGLGTCIVGWFNEKKVRSLLHIPASSRPVLIITI
                     GYPKNDEFPEKKRKPFEKIYNFNDYKGKMND"
     gene            complement(43028..46312)
                     /gene="mfd"
                     /locus_tag="PKI65_01960"
     CDS             complement(43028..46312)
                     /gene="mfd"
                     /locus_tag="PKI65_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOB77238.1"
                     /translation="MQKSIKTYKNIQISQVAGALSSIILLLFYRKTKANKGIYIANNE
                     DDALQIYNNLDYWIEQLQLNDKIYLQYLPSSYKNFDTFSINSSNEAMRLQCSELFADD
                     DIVSLVITYPEAIVEKIPKSENIIKNKTKLKIGDRVDLNFLIELLLYYGYVEVDYVYE
                     PGQFALRGAICDIYPFNANAPYRLLFKDETLSHVKLIDVVSQMSIQELTCIDILGSMD
                     ELNQTKVSIFQLLNINSIIWTSNFDLTNNNIEFFLNQLSDEINKEFIDYQEFITQINE
                     FTNVNFSNTNVNYYDKLIQFDVKPLPIFTKILKDYHLFCKEKIYEGYSIYFTSPHYPA
                     WERMQAIIEDMDIENDTEVLAYIKYLPTVFNEGFIDNQTKIIVVPEHQLFKKYLKPRL
                     LNKIHKKQVTNIEELMELQPGDFIVHIQYGIGRFAGLEQLEINGNKQEVIKLMFKDDD
                     VLYLNIQSMHKITKYICSDAKPPTLHKLGSIAWQNKKNKLKKHIKDIARELITLYAQR
                     KAMKGFAFQKDNYLQIELESSFEYEDTPDQLKAIEEVKKDMESDYPMDRLICGDVGFG
                     KTEIAVRAAFKAALDGKQTVILVPTTILAFQHYLTFSERLKEFPVTVEYINRFRTSKE
                     QKEILRQLAEGKIEIIIGTHRLLSKDVKFNDLGLLIIDEEQKFGVAAKEKIRQLKTNV
                     DTLYLSATPIPRTLQMSLMGARDISVLRTPPPNRKPIHTEVRTFEHKFIKKAIENEIE
                     RGGQVFFIHNNIETITEIANLIETLVPEARVGIAHGRMKSDEIETVTMAFLKGKIDVL
                     ISTTIIESGIDMPMANTIFINDGQNFGLSDLHQLRGRVGRRNQKAFCYILIPPTRFLS
                     SIAQKRLKTIEEFSDLGSGFSVAIKDLEIRGAGSIFGAEQSGFIEDVGYEMYQKILDE
                     TLIELRQELNMPITDNELNIPSSEITIDTDRSIFIPEFYAPTSIERIKLYRELNESNS
                     ENQLNDIIEMMIDRFGPLPPETIELIDAIKLKWLAQQLYIEKITLYKNILTLYFSSKD
                     YHKDKIIQKLPNILDFIQNHPKNTNMKQDTHRIILSIEPTRNFKMARKYLEEILEKKA
                     ETIENV"
     gene            complement(46430..46648)
                     /locus_tag="PKI65_01965"
     CDS             complement(46430..46648)
                     /locus_tag="PKI65_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77239.1"
                     /translation="MSHPHWEMICNKTSKRGRASQRSFFNYPVRQSLTPLRRRGITSY
                     SPLVEGMSHRDRGVKTSGDFSFVSFSFF"
BASE COUNT        16370 a         6749 c         6753 g        16850 t
ORIGIN      
        1 atgtaggtag gatgctcaat ctcgcatcat cagacagacg agcgtggtat gtatacagag
       61 acttgagagc aagtcctgtg tttatttaaa cattgaagaa tgttattacg ttatttaaaa
      121 aattaacttt caaacacttt caaaagcaac attataaaaa caacattatc tattataaag
      181 atctctaaat ctttatgtgt ttcgttatta tgtaaaaaaa gctttgtttt attttctttt
      241 atctaataag ttctaatgtt tcttgtccca gttgctcagc tacatatttt ctaataaaag
      301 gtgcagtgta cccttgatca atagacaaca tatcttgcaa agtgccatta tatataacat
      361 atccaccaag attaccaccc tcagggccta aatctattat atgatcacaa tgtgttatta
      421 cttctaaatt atgttctatc actataactg tgttgcccat atcaacgagt ctatcgagtg
      481 tttttaataa attagagata tcttcgaaat gtaatccagt ggtaggttca tctaatatat
      541 atagagtttt acctgtagat ctttttatta gttcagtagc tattttgacc ctttgtgctt
      601 caccaccgct tagagttaca gatgactgtc ctagtcttat ataacccatg cctatatcat
      661 ttattatttt tatacttttc tgtagacgcg aattaccttc aaaaaaatta taagcttgct
      721 gaaaagtcat ttctaaaact tcactaatat ttttccctct atatcttatt tctaatgttt
      781 ctctattata tctctttccg ttacatgatg gacaggtcgt gtatacatta tccattaatt
      841 tcatttcata tactagatat ccagtgccct tacaagtgct acaccttcca ttacctacat
      901 taaagctaaa tctacttttg ttataaccac gcattttaga ctcaggttga ttagcaaaga
      961 tttcacgtat ataatcaaaa attcctatgt atgtagcagg attacttcgt ggagtgcgac
     1021 ctatgggaga ttgatctatt atgattactt tatctatatt ctctaagcct tctaattttt
     1081 cataaggcaa acagttacga aaagatttat ttaaagcatt aaaaacagca ggataaagtg
     1141 tctctgtgat cagactcgat ttaccactac ctgatacacc tgttataccg ataagtttat
     1201 ttagtggaaa gtttactgtg atatttttta gattgtgtcc attagcgcca atgatggtta
     1261 agttttttcc attaccagtc ctatgatcta attttagttt aaatttcttt ttcccactaa
     1321 gatattgaca ggttaaagta ttgcattgca ttagctcttt tggagtaccc tgtgctatga
     1381 ctttgccacc tcgttcacct gcaccaggac cgaaatcaac tatctgatct gctgatagca
     1441 tcatgtctct atcatgctca actactatta tagaattgtc caaatcttta agtttaatta
     1501 aagatttaat taattgatta ttatctcttg gatgcaaacc tatgcttggt tcatctagga
     1561 tatataaaac acctgtaagc ccagtaccta tttgggtagc caagcgtatt ctctgggctt
     1621 cgccaccaga caaactataa gtaggtctat taagagtgag ataagaaagg cctacattca
     1681 acaaaaaatt tactcttgtt tttaattctt ttaaaagctc ttttgctatt aaattttgct
     1741 tttcagtcaa aatttttgga agcttgtcga tccaataaat cagatcagat agctccatat
     1801 tagctatctc agctatattt ttattatcta ttttgaaata taaactttct tttttgagtc
     1861 tagtaccttc acaggtaggg catatattga ctacttgaaa tgtattggcc cattttctca
     1921 tcatttgatt gtcagcttca gctaatttgt ttactatatt tatgatacca tcatagctta
     1981 attgataatt agatgagatg ccaatattat catattgcat atgtataatc tcatcggtgc
     2041 catacagtaa ggtttgtatt agttcatcag gaaaaacttc aattggatca tcgagtgtca
     2101 aatcatattt ctttaaaatc atttctaatt gcttgaaggt ccaattattt ttatacttgc
     2161 caaaaggagc aataccaccc tcgcgaaggc tttttctttt attaggaaaa attttatcta
     2221 tatctatttc attaacataa cccaatcctc tacaagtagg gcaagcacca taaggagaat
     2281 taaaagaaaa agtattaggt tctggcatag gatatgagat accactatct tcacacatta
     2341 aatttttaga gtaataagta gccttttcat catgtaatat tatcagatta ccattaccat
     2401 attttaatgc taattcaatg ctatttttca atcttcttat tttattatgg cttacatcca
     2461 attcatcgat ccataattcg atatcgtgag tttgataacg ttctagtttt atgccaggtt
     2521 ctattgtaac aagtttaccg tctactctca tttgttcgaa gccaagctta gcatagcgat
     2581 caaaaagttc tctataatgt cctttacgtg cttgtactaa tggggctaag atatgtatat
     2641 gcagtccttt aaatttttct aatatttttt ttataatttc atcgatagaa agctgcaaca
     2701 taggtttacc agttaaataa gaatacgcta ttgagacttt agcatataaa agtctcaaat
     2761 aatcatatat ttctgtaaca gtaccaactg tagaccttgg attttgagaa atagtttttt
     2821 gctctatagc tactacagga ggtattccag taatattatc tacatcaggc ctatcaaaaa
     2881 cacctacaaa ctgccttgcg taggcaccaa aagtttccat atatcttctt tgtccttcag
     2941 cataaatagt atcaaaagcc atagaagatt taccacttcc actcaatcct gtgaaaacta
     3001 ttaatttatg taaaggtatt tctattgaaa cattttttaa attatgtact cgagctcctt
     3061 ctattactag ataatcatta gacattattg ttttactcct aacataaata attaatttgc
     3121 aaagttaata aattttgatg aattgatacg caaaactact aatgctttta agcataatat
     3181 taacataata aagtaaacta attttttttt aacatacctg aatccgtgaa tataaaataa
     3241 aataaaaatt ttaaattaaa gatgttaatt tagagaagta aaaccatatt ttaaataata
     3301 atttatgaga acagcttaca tttgtgaatg ctatacatac tcctatcgga agatacggtg
     3361 gtgctttgtc taatgttaga accgataatc ttacagtttt aattattaaa gctgtattag
     3421 ctcgtaatct gcatgtagac acaaatggaa tagatgaagt agtattttgt tgctctagat
     3481 ttagcagata atagaatcaa aactttcgct cttgatagat tattaaatct taaaataaca
     3541 gatataagtt tttgtgagtt taaggatttt gatgctaacg agtatttcaa atattgtttt
     3601 ggcatagtga gaccagaaaa tgctgtcgtt gaggaagtga agttatcatt tactcccaaa
     3661 caaggcaaat atattaaatc tctaccattg catgctactc aaaagataat cattgataat
     3721 gatgatgagt ttgtaatcac acttagcata ttcatcactt atgatttcat tcaggagata
     3781 ttgcaatatg gtgatgaagt aaaagttata aaaccagatt cactagtaaa gacgataaag
     3841 caaatacacg aaaatgcttt gagaagatac taactagcaa attatataaa acaacgacat
     3901 aaataattat acgtaataat gaaaaatatt aaaaatacag agataaaatg attaaaatag
     3961 tttaaaattt tcgcttaaac ccatcaactc ttcaccccgt caccccgtca ccccgtctcc
     4021 cagtctccca gtctcccagt ctcctagtct cccagtctcc cagtctccnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnag tctcccagtc tcccagtctc ctagtctccc
     4201 agtctcccag tctccccgtc tccttctcaa cttatcaact aaatatatta atttcagaac
     4261 ttaaaaattt attaaatttg caaaaataat taaatggact cagatactat aaaaagttta
     4321 aatgcaagag gattgatagc tataaatatt gatagcaaaa cagagttgct ttttactaat
     4381 agatggcaaa agctcacttc ttataaagat gctaagcatg ataaagattt ttatatagct
     4441 tttcccgagg cgatagaata cgtagaggta caattcaaaa atgatagaaa acaattttat
     4501 agaaatacaa ctggaatgcc attagaaata ggttcattag tagcggtaga agctaatccc
     4561 ggtcatgata ttggagtagt atgcctgaca ggtcgcgaag tcatacaaca aatgaaacgt
     4621 aaaaaatcaa agaataacaa tggtgaatta ttgaatttgt atagaatagc aaaagaaaat
     4681 gatatagata aatttttaga tgctatcaaa aatgaggaga aagtcataaa gcaaactaaa
     4741 aaaattatca aagaaaaagg cttagaaatg aaattgatag atgtagaata tcagggtgat
     4801 ggcactaaag ctacttttta ttatatagct gatgagcgtg tagactttag agaactcatt
     4861 aaaactttag caagtaaatt tagaataaga gtagagatgc gtcagatagg tgctagacaa
     4921 gagacagcta aactaggatg gataggacct tgtggtagag aattgtgttg ttctacatat
     4981 aaatgtgtgt ttagttctgt atctatcact gctgctagta ttcagcaatt aacattaaat
     5041 cctcaacgtc ttgctggtat gtgtactaaa ctaaaatgtt gcttggcata tgaattacca
     5101 ctttacgaaa aatttattac tagtttacca cctacagata tcccattaat tactaaagaa
     5161 gctaaagcca catatgtaaa agctgatatt ttcaaaaatg taatgtatta ctcgtatgaa
     5221 aatagtgatt tattgtttgg cttgcctata tctgctgtta tacaaataca agaattaaat
     5281 aaaaaaggcg aaataccgga aaaacttgaa gaatttgcgg taattagtga atacactgta
     5341 gaagagagtg taagtcaaga agatttatca aaattagaag atgaataata atttttttat
     5401 tttaatattt atagtaatat tattacttag ctcttgcaag caggataagt attatgatat
     5461 acaatccttt gatgatgaag tatgggttta tacagaatgg aaggaatttg ctattcccat
     5521 acctgatacc attaatctat acaattttca tttgcttttg cgtacttctc aagagtatcc
     5581 gtatactaat ttatatttat ttgtgcaaaa tattcctcct actggatcta tatacgaaga
     5641 tacattattg ctgatattat atgatgaaaa tggcaagcct atcggaaaaa aaagcaataa
     5701 aaattatatc tatgattttt taataagaaa aaattatatc ttcccatcta ctggtgttta
     5761 cacactttta gtaacacatg gtatgagaga tagtattttg ccaggtataa tagaaatagg
     5821 attcaaattt gaaaaagtaa ataaaaatga aagataattt tttttcgatc ttcaaaactg
     5881 aagatgaaaa ttttaaaaaa tttttaaaaa atctttggat aatatttttt tgtattctag
     5941 gatttatatt tatttttttt ataggtgtat attatggact cttaggccct ataccttctt
     6001 ttgagcaatt agaaaatcct ttagataaat tttctagcga attaataagt gtagatgata
     6061 atgtgatagg taaatattat tatgaagaaa atagatcttg ggttagctac aatgagataa
     6121 gtccatacgt tatacaagct ttaatagcta ccgaagataa acgtttttat aaacatagcg
     6181 ggatagattt taaaggatta gctagggtat ttttcaaaac tataatagga caacaaaaag
     6241 gtagtggtgg cggtagtact attactcaac aattagcaaa aaatttattt aaacgccctg
     6301 ataatattaa caaaatcaaa ctaataggta ttaaatttag agaatggatg actgctataa
     6361 aattagaaag aaattattca aaagatgaaa taattgcaat gtatttaaat actgtgcctt
     6421 ttggttatca attatatgga gtcaaatctg cttcattata ttattttggt aaaaatccca
     6481 atgaaatcga tatagatgaa gctgctattt tgataggtat gttaaaagct actactagat
     6541 ttaatcctat tcttaaccca gaaaatgcca aaaatagaag aaatattgtt ttgaagcaaa
     6601 tgcttaaaaa tcaatttatt aataaagaac aatatgattc attaaaggta aaggaaatca
     6661 caaagccaga aacaagtatc acaactaaaa ctgacgacct tgcaccatat tttcgtgaat
     6721 atctacgtct gtacatgact gcaaaggagc ctgatcataa aaaatataag aataaagctg
     6781 attacaaaaa ggcaaaagaa ttatgggata atgatccatt atatggatgg tgtaataaaa
     6841 atacaaaacc tgacggcaaa ccatacaatt tatatagcga tggactacgt atatatacta
     6901 ccattgatag tcgtatacaa agatatgctg aggcagctgt aagggagcat ctgagtcaag
     6961 atttgcaaaa aaaatttttc caagaagcta aacgaagcaa atatgctcca tttgtaggag
     7021 caacttctca agccgaagta gataaattga tggatatggc tattaaacgc actgatagat
     7081 atctcagtat ggtcagagat ggttatagtg aaaaggaaat aaataaagct tttcataccc
     7141 ctacagaaat gagagttttt agctggcaag gtatcaaaga tacagtgatg acaccttatg
     7201 attctataat atatcataaa tatttcttaa gagcaagctt cgtagctata gaaccacaaa
     7261 caggctatgt aagggcttat gttggcggac ccgcatatag ttttttcaaa tatgataatg
     7321 ccgctgtagg caaaagacaa gtaggatcta cttttaaacc ttttgtatat gcttcagctt
     7381 ttatcaatga tcctactctt acaccttgta ctaaagtgcc actgatacct gttacttttg
     7441 atatgcccga tggttctcaa tggacgccaa gaaattctgg aaatgtacaa gaaggtacta
     7501 tgatagaact ctcgaaagct ctagctctat ctattaactg gatttcggct tttttaatta
     7561 aagaaaaaac aacacctgaa gctgtagtag aattagcaaa atcgatgggt attactgata
     7621 ccataccgcc agtaccatct atttgcttgg gtacagctga attaaaatta attgagcttt
     7681 taagcgcctt atctactttt gcaaattcag gagtagcaca aaaacctatc atggttacta
     7741 ggatagaaga ccgctttggt aatataatta gtgttttccc ttatggggtt actcatgaag
     7801 ttatggacga atttactgct tacaaaatga tatctttgat gaaaggtgtt gtagattatg
     7861 gtactggtag cagaattaga tatatttata aaattcctta tcctgttgct ggtaaaactg
     7921 gcaccacaga caataatagt gatggatggt ttatcggtat tactccaaaa ttagcagctg
     7981 gagtgtgggt aggcggtgaa gatatgcaga tacattttcg tagtatggct ttaggtcaag
     8041 gtgctagcat ggcattacct atttggggac tttttatgca aaaagtctac gctgatgact
     8101 cgttaaatct gtataaaggt gattttgata gaccagcagg catgagctac gagatgctct
     8161 gtaatccatc cactacagat gctgatggct cgtctatgcc atttattatg cctaagaaga
     8221 aaaaaacttg gaattaaaat gcgtagaaaa accctacaaa ttcccattgg caatatatta
     8281 attggtagtg aatatcctat tattatacaa tctatgacta atactttaac atctgatatt
     8341 caaagtactg taaatcagtg tattagatta tttgatgctg atgctaaact cgttagaata
     8401 acagctcgca atctgaaaga agctgaatgt ctaaaattga ttaaaaatga tttaataaat
     8461 atgggatatg agctacctat tgcagcagat attcatttca atccacattt agccaaagta
     8521 gctgccaatt atgttgaaaa aatacgcatc aatcctggta attttgttaa tttatttcct
     8581 aataataagt catatactaa tgagcaatat aagttagaag tagatgctat taaaaatgaa
     8641 atcgaaaaac ttttacctat cttagtagct aataaagtag ctgtgagaat aggtgtaaat
     8701 aaaggttcac tgtctcaacg attaattgat aaatttggta atactactga agctctcgta
     8761 gaatctgcta tagaaaatgt aaaaattttt aataagttaa actttaataa tattgttatt
     8821 agcattaaaa ctagttctgt ttatgagaca atagctgcta ataggctatt agtatctaaa
     8881 ctcgacaaac taggacttta ttatccgatc catctcggcg tcactgaagc tggtatagac
     8941 aaagagggta ttatcaaatc ctcagtagcc attggcaatt tattattaga tggtataggt
     9001 gatactatta gggtttcttt aactggtgat ccagtaaaag aaatttctgt agctgagaca
     9061 attatcaaga cctatcaaaa atttttacct ttaccaccaa tgccgactta tagttatact
     9121 ttagagaatg ctcctaaaat atttactatc aatgatttaa ctactgaaca tcttgattta
     9181 aataatctat tcatagacct tccaaaaaat aggcctttcg atttcatagc aaataaatta
     9241 aaaactgtcg aacaagtgaa tatcaaaata gatattgata aatttgatct ctatgaatta
     9301 agcataattc ttggtagagg ctggatagat ggatggctaa aagatgttta tttagtttgt
     9361 gataatctaa aaaatgaaaa agtaaatgac gtatatcaaa ttttacaagt aactggtaaa
     9421 aaaaagttac tcactgaagt gatatcttgt ccatcgtgtg gacgaaccat gtttgatatt
     9481 gagagcatta ctaggcaggt aaaaaaacgt tttagcatat atcctggact tactttagct
     9541 gtgatgggat gcatagtaaa tggtccaggc gaaatgcgag aagctcaagc aggaatagta
     9601 ggtaatggaa ttaataaagc agatatatat gtctttggta gagtagtcgc taaaaatatc
     9661 ccagtagata aggtagtaga cgaatttgaa aaacaattaa aaatacataa tctgatataa
     9721 aaaataattg attttttgtt ctcaaaaaat taaataatat agtaccaaaa acagagagaa
     9781 aactaatcta ttatatttta attaaaatca tttaatgttt agatataaaa ttatgatttt
     9841 tgtattaaat caaagaagat atttattatg atataaatca tatagttaaa gttgaaatta
     9901 attatttata attttgcttt tgcaatttta ttacattaat gaaaaacaac tttaaacaaa
     9961 cttttattaa tctcctttca ctatttatgg cagtatttat gatattgcta atagtcaata
    10021 atatagtttt tttgcatgtt cataaattac caaatgggga aataattgtg catgcacatc
    10081 cttataataa atctaatgac actgagccta ttaaaaatca tactcattct aatagagaat
    10141 atttagtatt agatcaatta aaaaatatat ttgttattac atttatattt ttttcttttt
    10201 tatttttttc aaataaaatc ttaattttca aaaacaaaat tgtttatcat ggctttgatc
    10261 atgataaatt ttataaaaat aaagcacctc catacttcat attaaattat taatagtagt
    10321 actattttat tcattttata atttgcttaa atgatcatat aagcagatta agattattaa
    10381 ttcaattata aactaaaaaa ttaaaattta tgaaaaacaa aaaaatagtt tttatattat
    10441 gtatcatgtt atgtacatta aatatttatt cacaaaaaaa agaaactgat gcaaatataa
    10501 tagggcatgt aatgtgcgat ggagagcact taccctttgc atcaatatat attaaaggaa
    10561 caaatatcgg aacaactagt gatgaaactg gacattataa aataataaat atcccaatag
    10621 gtactcatat tttagtagct aggtctgtgg gatacaaatc acaagaaata gaaatagatg
    10681 tttgcattgg tgaaactaaa gaaataaact ttgaattaga aaaggatgtt ttaaatttat
    10741 ccgaagtagt tataacaggg gagaggaaaa tgatatataa gaaaaaatca tcaattattg
    10801 ttaacacact ttctcctaaa ttatttgatc tgactcaatc tacatctatg ggagaaaatc
    10861 taaattattg tccaggatta agaatggaaa ataattgtca aaattgtgga agttcacaag
    10921 taagaatgaa tggtatggat ggtccttatt cacaaatctt aataaatggt cgtccaattt
    10981 ttagcagttt agctagtgta tatggcttag aattaatacc tactaatatg atagacagaa
    11041 tagaaatagt tagaggtggt ggatcagctc tatatggtag taatgctatt gcaggtacta
    11101 taaatataat tctaaaagat ccttcaataa attcttatga atttggtata agtcaaggaa
    11161 ttattggtat tgggatagat agtactggag gcatagcaaa tgatttttct attaaattca
    11221 atacttcgat agtatcgact aataataaaa ctggtatgac tttatatggt ttttatcgca
    11281 ataggaatcc ttttgatgca aataatgatg atttctctga attaagttta ttaaaaaatc
    11341 tttcaatggg aactcgatta taccataaaa ttggaactaa aagtaaatta tcgacagatt
    11401 tttttataat ttcggaaaaa agaaggggag gggataaatt tgattatcca gttcatgaat
    11461 caaatataac ggaatctgca gaacacttta ttactacggg agccttaaac tatgaaagat
    11521 attttagaaa aaatgatctt ttatcaatat atttatcagc tcagaaggta gatagggatt
    11581 cgtattatgg agcaaatcaa tctttaaatt cttatggtaa aactcaggat gtttctttgg
    11641 tagtaggtgc tcaatataat gtatattttg ataaatctaa tttgatattt ggtgtagaaa
    11701 ataatggatc atggctaaaa gataaaaaat taggatatcc agatatagac aatgcaatga
    11761 taataaatga tattattata aatatacctc atgttaataa tactatattg acaaatcaaa
    11821 caagtaatat tatagctggg tttgctcaat atgaatataa ttgggaaaga atgaaaatag
    11881 ctattgggtt acgtttggat aagtataata taaaagataa aaataaatcc aaaaataata
    11941 tatctggtaa tatacttaat cctcgtatta attttaagta tgattttaat tctttctgcc
    12001 aaggtcgcat tagttattcg cagggatatc gtgcaccaca gatatttgat gaagatttac
    12061 atgtagaaac ctcaggtgct aggcagataa ttcatataaa tagtcctaat ctcaaaatag
    12121 aaactagtcg tagctatatg atgtcattag attttaataa aaatttttct aatatatata
    12181 ttaacttttt gatagaaggt ttttttacca agttaaataa cccatttacc aatgtttttg
    12241 gtttgccaga ttctaatggt atagtgattt atacacgtat aaatgcagat gatggagcca
    12301 gtgtaatggg ggttaatatc gaatttaata ttattccttt tgaaaaatta tcaataaata
    12361 caggatttac tttgcaaagt agtaaatatg atgtgcctca agagtttaat gagaagagat
    12421 ttatgcgtac tccaaataat tatggatata taatattaga ttacaaacta acaaaaaaat
    12481 ttggaatagc tttatcgggt atctataccg gtaaaatgct cgtcccatat tttggcaatc
    12541 aaattgctga tcctatggca ggtgaactga gaaatactca aaactttttt gattttggat
    12601 taaagacatg gtataatgta aaaattaatg gtgctacatt acaattattt ataggtatta
    12661 aaaatatatt taattcttat caaaaagatt ttgatgttgg cctcgataga gatcctggtt
    12721 atatttatgg gccagcttat ccaagaacaa taaattttgg aattaaaata ggaaatgcta
    12781 ttaaatgaaa tattttattg aattcaagtt attactaata ttataacaag attcttgtgg
    12841 tgtatgatca tacgtcctaa tgatgcacaa attgtatggg caattcacta attgtccata
    12901 taatttgtca taaacctttt gacaaatcaa gataatcagg ataatcaact ggaaatctgt
    12961 gattctgaca ttatatgctc tttgggaccc ctctctctag gtcatctgtg atgcccatgc
    13021 ccccatcttt ttaacttaat aatatatata acttatttta tttctttttt ttaattaatt
    13081 aaactcttat gttgtaagtt gtaagttatt ttctccatag gtttacatta taaaaccaaa
    13141 aaggcttata ttcattattt ctatataatt tccaggtata catcttggta ttgggtatat
    13201 aagtacgata aaaataataa cttttagcat acatttcgtc tttagaacca taaagccaga
    13261 tttctgtatc atatagtctg taaatataat ttggaggccc ctgcacgata tagatcaatc
    13321 ctttatctgt ttgccagcca ggttgaatat ctgtaaacca tttatcagca tctgtcactc
    13381 tagtataata ttttttaatt acttcagctg ctcttttggc ctcgcctatt tgtgctagcc
    13441 agaatgaatc tactgttttt ttaggatatt cagattttag catactattg aattctgtat
    13501 cagtagtaat ataccttagt ggtggcacaa gttcatatgg ataaataatt tcaggaaaat
    13561 gggctggtac tactataaag aatatacctg tagaaaaagt atctatcgat acattaatta
    13621 gtgtatttaa taaatctgaa gtagctaccc atgattgtga tgcagaaata gaatgatctt
    13681 tgatcttttc tatttcaatg tcttttaagt tcaattcagt tacaaatggt ggggcagcta
    13741 caggagggaa tttatataca gaaaacacca aactatctat atcaaatgca gaattatata
    13801 ttttaaaagt atcacttatt tttaaaaaat gtttatcttg tagattagta taaaaccatt
    13861 gtgcactata tggggaggtt ttatctatat tcaaatattt gacagtataa tttttaccaa
    13921 aatctacaga tttgatgaat aatctgcaat aacccttgaa tggatgatta atctgaagtt
    13981 ttatggtagt atcccatcgt gatattgtat cagcaataga aattttaaat attgaggatt
    14041 tcaaaatttt atctaatgaa tcataaagat caattttgca ttctattctt gcaattttat
    14101 aaaaaatact atctggcgtt ggtaagttga cgaaatgtaa attagtagta tctatctcta
    14161 tattcaaaat actaatagag tcagacaagt gataaaaaaa ggcattagat ctaatgttat
    14221 aaggtgtagc ttgataaaat tcataaaaag gatagctagt agatatcttt gaggttttac
    14281 aacttataaa tattataata aataaaagta aaaatataat ataatgttta attttcattt
    14341 ttttatagct tttgaaaaat caatacgatt tttaatggct ttacctaagg ttaactcatc
    14401 gatatattct atatcaccac cgatagatat gcctcgtgca atagctgaaa aatataacga
    14461 attcacatct ttcaacatat taaatatgta ataagcagta gtatcgcctt ctactgtagc
    14521 tggcaaagca aaaataattt catttatgtc attatttctt aaacgctcgg gtatatcatt
    14581 tacaaatatt ttatcaggtg ttatgccatc aataggagag attaacccat ttaaaacatg
    14641 ataaatccca ttatatactc tggatcgctc tatggctatg agatcttgaa tattttgtac
    14701 tacacatagt atggtagtgt cgcgagccat atcagagcag atattacata tttcagcatc
    14761 agaaacatta tgacattgcc gacatagaaa tagatttgta ctaagcaatg ctatactttc
    14821 actcaatttt tttatatatt gaggatcttg ccttattaaa aacattacca aacgtgtagc
    14881 cgttcgcttg ccaatacctg gtaacttagc taactcatta atagcattag ccattgtttt
    14941 ggataagttt tctaaatcca cttttttatc aaaattaatt aaaatatttg aattggtaaa
    15001 taataaatga ccgtatgata ttaagctaaa aatgaaggaa ttgttaaaaa tatgataaaa
    15061 aaaattaaga tattttattt ctaggatgac aactttctat aacatctcgt aagtatgaca
    15121 tagaaatatg tgtatagatt tctgtagtag tgatagatga gtgtcctagc atttcttgta
    15181 tggcaaataa atctgcacca ttttcgatca tatgtgtagc gaagctatgt ctgaatgtat
    15241 gagggtgcac atttttgcta atacccgctt tagcagcaca atcttttact attataaata
    15301 tcatttctct actcaatttt ttaccacgtc tattcaaaaa aatatattgc tgatattgag
    15361 ttatcacagg atagtggggc ctgctatatt ctatataata gtgtagagct tttttagctt
    15421 tactgcctat tgggacatat cgttctttat cacctttgcc tatgaccctt atcattgtct
    15481 cttcttcgaa gtagtctatc attttcaagt tgacaagttc actgacacgt aatccgcaac
    15541 catataaaac ttctattatg gctttattgc gatgcccttc aaacttgctt agatctatca
    15601 ctgctattat ttgctctatt tcttctacag ataatacttc tggcaatttt cgagttaatt
    15661 ttggcaattt taatcgttca gcaggatttt ctttaattat ttcttcttct aataaaaaat
    15721 tataaaatga tctgatacct gagataatac gtgccaagct actactttct agctctaatt
    15781 ctgataaaaa acttacaaaa ccttctaaat cttttaaatt agcgtctaag aaagatttat
    15841 ttatattatt aaagtaattc atcaattttt tcacatccct caggtatgca tctatagtat
    15901 ttttactatt atgcttatcg agcatcaaat aagtgtgaaa acttttaaga tattcttcat
    15961 tcattttttt aaaaaaaata ttaaatgccg taataattat gtggtgagag atttaataat
    16021 ttttctttta tttcgtcatt tacatctaat tgattaataa aatcaacaaa atcatgtttt
    16081 tttaaacctg atttacctct tgtgaattgt ttaagggcct cataaggatt gggataacca
    16141 tatctgcgta agatagtttg tatgccttcg gctattattt cccaatgatt atctagatct
    16201 tcatcaattt tagatttgtt tacatctaat ttattcaatc caaatttcaa agaattatat
    16261 gcaatgagag aatgagcata gggtactcca atatttctca aaacggtaga atcacttaga
    16321 tctctctgta atctagaaat tggtaattta ttagataaat gctctagctg tgcattagca
    16381 atacctaaat tgccttcagc gttttcaaaa tctataggat tgactttatg tggcatagta
    16441 gagctaccga cttctctttt atctattttt aaatgaaaat aatctaacat gatgtaaagc
    16501 caaaagtctc tactaagatc taataaaatg ttattaatac gtttcaaagc atcaaaaaaa
    16561 gctgcagaat aatcataatg atctatttgt gtagtgtttt gcaatcgtat aagtcccaaa
    16621 ctactaagaa actcattgct aaatttaacc caatctatat ctggataagc cactatgtga
    16681 gcattgaaat tacctaccgc tccgccaaat ttaccagcaa aaggaatgaa attgatcaga
    16741 tgaagttgat tatttagtct ttcgataaaa acagctattt ctttaccaag tcgtgtaggt
    16801 gatgctaatt gaccatgagt acgagcaggc attattattt gccaccattt gttagaataa
    16861 tcatttaaaa tatttaaaat ttcttctata gatggatata aaacttcaaa ccatgcatct
    16921 cgcatcatca tggggaaagc tgtattatta atatcttggg aggtgagccc aaagtgaata
    16981 aattcttttt ctttttcgaa tcctttttta tcaaattgta ctctaagcca attttctaca
    17041 gctttcacat catggcgtat ttcattttct attgctttga tctcattagc tgcttggata
    17101 gaaaaacttt cgtatatcga tagtagccat ctttctcgtt cttcatttaa aaaattttct
    17161 aattctggta atggtatttt acataatgct atgaaatatt ttatttctac atttaatcta
    17221 tattttatta atgcaaattc agaaaaataa ttttctaaaa tttttgtctg ttcgcagtat
    17281 ctaccatcta ttggagatat ttgtaataaa ttggtcattt ttttatacaa atataaagta
    17341 aaaaatgtaa ttaattaata catttgaaat tgatgatttg tattaatttt aattatatta
    17401 atttgattaa gtgtataata aataaaaact ataaccaaaa ttttagaaga ttaaaaaaaa
    17461 taaggagtca agggtctaat gagttgaaat atttatttta atattttaca gcatcaaaag
    17521 tggcaaaaat gtttcattat tatctaatct gactctgatg taatagattc cattagataa
    17581 attatttaga ttgtttaaag gaatcgaata attaccattg ttcatataat catttaaaat
    17641 attatgaact aagttaccat taatatctat tatttgtaga gaaactttat atgaattatt
    17701 caattctaaa tatatttgag tattctcgtt taaaggatta ggatataatt gaatttgatt
    17761 acctgctaac acttgagtat tgatatcaga catcgataaa atattaatat catctatata
    17821 aatattgttg ccaccattag aataaaagct aaatttcaat ctaagattat cataattttg
    17881 aacataagat gattgaataa taaagctaaa ttcggcccat tgagttgtgg atgaaggtgt
    17941 aaattctgat tctataggag ccgtagttat aaggttttga ccagatttta ctaatctact
    18001 ttgccatgtt ttaccacaat cattagaaat ataaaccgtc aatttatcat agatagtatc
    18061 agcaggagtt aaaatagtac ttggtattat tttaccagaa taagctactt taaacgaaac
    18121 tttagcatga ccagaaggta aagaagtaag attgtaagca ggtgtgatga actcgtcttt
    18181 actgccagcc acattatcta tgaaattagc taaatagatg catttatttc cagaatagct
    18241 tgttccagtt tttatttgcc atacggagcc agtatcatta attactataa aatcattaat
    18301 aggagtgtac tcaaaagatt cactaatagg tgctgtgtta ttggtcgtag agctataaac
    18361 atgaatataa ttggccttag taagcgtgtt agttccttga gcattattta cctgaaatgt
    18421 aacagaataa acaccaggac tattgtatat aatagtcgga ttttctagtg tagaattatc
    18481 aggattacca ccttgaaagg tccaattaaa tgttgtagct gtaccattaa atgaagcatt
    18541 gaaaaagctt atttcactac cagcacagac atattgagta ttagagaaaa aatctgctat
    18601 aggtgcacat tcatgtgaat cttcttctaa tacacctgtt gcttgtaaat tttctacaga
    18661 ccataaatta cgcctgtaac cattaagtgt agcatctatt ctttgatatt ggcccaatgt
    18721 gaatatatta ttacaatagc cccattgata gtccatataa ttttctatca tatctggttc
    18781 atctggatct tcagtacaag tatttctaga tcctggcgta aaacaactaa ttgatgtatt
    18841 ctgtgtagct tgatctactg gtggtgtgtc atctacctcg tcacccgttg ttgctaacat
    18901 agacggacag ccactgctag taggtataag tcctaatatc aaattttgaa aagtatgata
    18961 taaattgaaa taatggccag attcgtgagt aaaatagcga tttaccatat ttaaaggcat
    19021 gtctgcaaaa ttttcagcag tacctatacc acctatacaa tcatgacgaa tcagtacacc
    19081 atctatatct gtatctccac ctgtaagagc ttggctaagt ggattgtcgg gaggaaaggg
    19141 cgacatacca cctatcaaag ctccttctgg caaagctaac ccttcgggat aaatgaaatt
    19201 aactacaaaa atattcatat attttgtagg atcccacaca tactttttca tagtattgaa
    19261 atatgcataa ttagtctctg gtgtgtaatg cctcactatc ccatcagtgc agttaccatt
    19321 aggatctttt tgggctagac gaaattctat attacagtta gcagctctac ttttaaataa
    19381 gtcataagta ttaacagtgt ctgcattttg tttattaaaa tcaatattta tcttttctaa
    19441 tccatccagt atttgagctt tattaatatt ttcgctacca taagtatgta ttatatgaaa
    19501 tactacgggg ataatccttt taccatcaat cagtgtatca gttttttcaa atgaaaaatc
    19561 tttattaaaa agtgctttga agttatcctc ataaatcata taatctacaa ttagatgggg
    19621 atcttctatc acttttagct ttaataatac atcatcagct ggatataaat aaatattttg
    19681 gttattttgc gttttagcta ttaaacaagc taaaatcata aaaaatgaaa aaataatagt
    19741 acttttcata attaaatttt tttacaaaaa taaaaaaatt atttaatttc attctctttg
    19801 ttgattcaaa atattttttt taattttgca tataatctaa tttaaatttt tacaaattag
    19861 atttatccat aattatctaa gggagcatat tttttatgct ccctttaatt ttatataata
    19921 ctaaattatt aaagattaaa aaaaaactac cttaatcgat gtattaaaga gataatttta
    19981 ttcagggtta ggtctaccaa gtggtttacc gtgtttccac agataatcta attcttcttc
    20041 ctcttttttt tgttttgcaa taaaatcttt tgcatctaga atatctaaat ttggttgccc
    20101 agcagctacc catgctgtat accagaatga agctacattg tgtatagcac gtctaaaatt
    20161 tctttctatc atattatgca gtagagcatt aaactcttgg acaaattctc tagaatattg
    20221 cttgatcaat atattatttc tatcctccat agtataaatt tgagagggcg tataatgctt
    20281 tagcatataa gcatatacac ttaaaacggt atcactttgt agataaccta atttaatcca
    20341 gttccaaaat tcatctactg gtttggatat atatttagct ctacctacga agagattgaa
    20401 attatcatat agcacctcta cagctctgct ttcccacaat gcatgtattc ccttttcttc
    20461 tatagttcta ccatcgtaat attcgttaca atgtaatgga acatgtgcat cagctacata
    20521 atgtcctata ttagcactta gataaagtat tttgccagca tcaccttctt tgaaggcgtc
    20581 aacaagtcta taatacattt ttattaggtg gtaaggtaag atgccataag cataaagtgt
    20641 atcttcagta tattttttca cagcatcatt ccatgtcttt ggaaccatat caaaaggata
    20701 ttgtccatag tgatttatat ccatataatg acgagctgct tctataggat ctacgtatga
    20761 acgcttatca ggatctactg catgctgcga aatataatcc ttgtgtcttt tataaaaacc
    20821 taccatctct ggtggcaaac aataaatagc atgttcattg atcctaggat gggcaaaaaa
    20881 tccccaacca aagagattta tagatatagt tgtagttatt aaaattatta ttaattgctt
    20941 cgctaattgt ttcatttttt tttaaaaaca tgatttattt attcaaaatt aataaaaaaa
    21001 attaataaaa aaaagttttt ataatctaat ttttattagc ttaaatctat gattttttct
    21061 ccatcaaaaa atggtatgac atttgtaata tttaattgta atgagtaagg tactatatgg
    21121 gctaaattta atttgtcgtg aagtctcttg taatgagaag ctttttttat ataagcttct
    21181 aaattattat ttgacatctc ccataaatct aatgcgatat atgcactatc ttctatacat
    21241 tgataaccat gaagcaataa atattgtaca aaagcacctg cacagatagt atcttctaag
    21301 ttagggaaat ccttccatcc acttgaaatc aatacaatag gtttattttc ttgaatcagg
    21361 tggtcggcta atgctctaat attaagaaaa gatcctaaat aaacctcagc tatggaataa
    21421 gctctcaaga tagtcttagt accattagta gtgaaatatg caatagattt acctctcaca
    21481 cggtttatac taaattcatc agcactattg cccaaatctg catgtggtaa tttgatacca
    21541 tcgcgttcag cagctaaaag ataacctttg tttttgtatt catatgtttt agcttcattt
    21601 tgtaaaggta ttatttcttt cgctccattc ataaatgcag tgcatatact tgtagtagct
    21661 ctcaatacat ctattactat actattaaat ggtggctttg ttatgacctt tggttcatcc
    21721 aaaaatagtg gacttaaaac aacttctaca cttaacattt tttttacaaa aataagtaca
    21781 acttatcaat aaaataaata tttctaatca ttaattttaa taagatatct acacttgttg
    21841 tatttttatt ttagctttta tatttgtata cttggtctag tattgtagtc attataaatt
    21901 atttgtaatc tgtattaatt ttttataaat acattgaaat ttggatataa agaatcagct
    21961 ttttgtaaat aaaaattagc ttttacactg tcattatttt ttagatgcca ttttacttga
    22021 aaatatagca tatctttatt atcaggttct atatattcat aaatatttaa gatagattgc
    22081 atagcaggat tattttgttg aaaaagattt gcagaatatg aaaaagatat tataccgata
    22141 aaagataata aacgttgtgc tagtgcataa ttcatttcgt caataatatg attagaattt
    22201 agagaaatat ttctaagaga atctatatat tttattagtt ttttatcatc agcaaaatta
    22261 aaaaaattaa taattttttg ttgttctgac cattcttttt caaaaatatc gaataatata
    22321 ggcatagttt ttttactaaa aatttcataa gattttatca attgatcttc agatgttaaa
    22381 aaattgaatt ttttcatact taatagagta gggtagagaa aaaattcagg aatagagtct
    22441 aatgaataat tgggttgctt tttcatttta gttttatcaa aaatcaacat tttaatagct
    22501 aaatctacaa tgttttgagg tggccaatgg tgtttaccat caaaaaaaat aatagctatc
    22561 gaaggcgaat ttgcatattg agtaaaaaga tgaaaaaatt ctgaaaaatt aaaatctttt
    22621 atacccacga tatgaacaat aggaatcata tttttagaat aaaattctgg attgtaagca
    22681 gatgcaccag tcaatatgag tcctttggct ggttgaaact gttcctgtac tatagtagcc
    22741 actctactac cacctgagaa accaacaaaa aataatttag cagtatctac tggtaatttg
    22801 cgatttatat ctcttatgaa aatttcgaaa tatgataatg aaaaattagc aggtgtctca
    22861 ttagatgata gtgtatatcc aataaatatt acacctaaac tgtctgcaat agatttatac
    22921 tttttcattg ctaactcagg atgaccatga gggtcaaaaa atatgaaggt tggtaatttt
    22981 ttatgtttat atctctctgg tatatatgct gaatatttgt aattagtatt tgctaacttg
    23041 atactatcta ttaatttgcc tcgatgtatt ttgtcacaat agatacattc gctattttta
    23101 caagctatga aaatagctga caatgagatg attaacgcta ttgaaactgt tcttttcatt
    23161 ataaattttt tttaaaaaat attaatagat aaaattaaat aaaaaataaa tcttaatttt
    23221 gggaaaaata aacatgccat tgtcgagttt actattaaaa ttattaggat ggaaagtaga
    23281 aactgaagag gatgtaacta aatataaaaa agcagtacta ataatggcac cacatacttc
    23341 tatttatgat tttataatag gatggttggc tttaaaaaaa ttaaatgtaa aagcaaaatt
    23401 tttgattaaa aaagagttct ttttttttcc attatcaata attttaaaat ctttaggagc
    23461 tataccagta gatagaggag atcccaaaaa cttaccaagg tatctagtta aactaattaa
    23521 taaatcatca gaaatgtggt tgataataac tcctgaaggc actcgcaagc ctgttaaaaa
    23581 ttggaaaagg ggtttttatt atatagccga tagtgcacag atacctatca ttccttctta
    23641 tttagactat caaaaaaaaa tagctcatgt tttgccagct ttttatccga caggtaatta
    23701 ctattctgat atacaacctt tcattgatat tttagcaaat gtgaatgcga gaaataaaaa
    23761 taattttgtt ttacctaaaa attaatataa aatgcaagaa actaattttt tagctttatt
    23821 tagatatcct agagaatgga tagaagattt gataaacgaa ataaataatg ctaaaaattg
    23881 gatatatatt gagatttata gatttgtaaa tgacccgact ggcatcagag tcagagatgc
    23941 actagtcgaa aaagcaaaag aaaatattaa agtttatgtg ctagtagata gtttcggagc
    24001 aaaatcagac acaaattttt ttaaaccact gatagaagct ggtggacaag tacgcttttt
    24061 cacaccgata cctatatcat tagctatatt ttcagaagct caccgaagaa atcatcgcaa
    24121 gatcatagtt atagatcaaa tatcatacct cggttcggct aatatttcaa attattcact
    24181 cgattggcgc gagagtattt ttaaatttga tgatcctgat ctagcacgta gattggttta
    24241 ttttataaaa ttatatttca aacattataa gacttatact tttaatcaaa aaaaatttca
    24301 tcgaatacat aatgtaaatg gaatggaatt aatcagagaa ataccatcaa tatggaagca
    24361 aagaatcaga aaaaaactta attggttatt agataattct caaaaatcta taaaactggc
    24421 aactccatat tttttgccta tgggatctac tatagatgct ttgatagaag ctgccattag
    24481 aggtgtagat acacaaatat atttgccttt tcgtagcgat gtaagctcta tggatgtact
    24541 tagaaattat tatttaggaa aattgtggag agcgggcatt agaatatttt tttatttacc
    24601 tacaaacttg catgccaaat tacttttgtt agataatgaa caatatatga taggtagttg
    24661 taattatgat tatagaagct atctgtatat gcacgaaata aatatggtag caaaagatcc
    24721 agtagtagta aatgaattag aaaaatattt tagtgaacta aaacaagata gcatagaatt
    24781 tgattatgat cgttggcgag aaagacctgt gctagataag ataatagaaa aaatattaat
    24841 accttttaga tttttattct gaataattgt ctaggtttat gttttttaaa tattttgttg
    24901 tgaaactaaa tatctaattt atttaatttt ttcataataa agaacagaga gtttaaaaat
    24961 aatttaaggt acaataattt tatattttca gtataaatag ttttgttttt ttattgtgaa
    25021 taatattttt tttatttttg taaattaaaa aaaatattgc aatagcaatg aaatattttt
    25081 gtttatttat ttttatcttt ttaatttctt ttaatttatt ttcacaatta ggcgatagca
    25141 ttgataaaag caaaccaatc ttgcatgtat ttggtaattt tcaatatgat gctacaaaag
    25201 gtgcaaataa aaaatttggt tttggcattg gtagagctta tttaggttat cagtatcaat
    25261 ttaatgataa ttttattggt caagtaatag tagaagctga taaaccaact acaatagaca
    25321 agatagaagt aaaagattct ttagataatt tgctatatgt catttaccaa gaacgaatag
    25381 gtagtaatta tacgatgcaa ttaaaaatag cctcgttaca atggaagcca aatgacaaaa
    25441 tcaaaataca gatcggttgt atagaattaa ataattacaa tactcaagat aaattttggt
    25501 cttataaata tattagtgaa acttttcagg ataaatattt caaattgcct agtgctgact
    25561 taggtattat tagttatttt tctattaatg attacataga tctggacctt gctctcacaa
    25621 atggagaggg taatcgattt gatcaagatt tatttggtga tttacaagtc gcttctggta
    25681 ttgatattaa accaattgag agtttgcata ttcgttttta ttatgattat tccaaatcta
    25741 aagattcact tagaccagct gagaggcaac ttttttctat ttttactggt tatcaatatg
    25801 ataatagatt caaaatagca gcagagtata attatagaaa aaacaatatt tttattaata
    25861 ataatgattt atatggtttt tctatttata gtatttataa tttctataat aaatttagct
    25921 ttattgctag gtttgataag ttatatgtaa aaaatatata tattgacaat gaaagctggt
    25981 acagtcatta tccaggtaat ggatatgtta tagggtttaa ttataatcca gcgcaaaaaa
    26041 taaatttttc tattaattat caattatggc aacctaaaga caagaatatt tttctacaac
    26101 ataatatttt tttcaacttt gagtttaaga tttgataatt ttttcaatta tttagtatat
    26161 catttaaaaa attatctata tctcttctat cttttttggt aggtctacct ttccatttcg
    26221 gcttgtatgg agataaatca taacgtatca tatccgattt ttgatactcc tcatcaggtg
    26281 ttagatcttc tataaattga tttacgattt taggcccgat tctctgatgt ggtaaagcag
    26341 taatttttat agtttttaca taataggtac attttatttc tactatgtct ccttcgtgca
    26401 cttctttaga tggtttagct atttgattat ttacttttat tctagatttt ttgcattgtt
    26461 cagatgctag cgaccttgtt ttaaataatc tcacatacca taaaaattta tctaatctca
    26521 tattttattt ttctctgaaa tatattatca ctgggttgcc atcaaaaccg aaatgttgac
    26581 gtattttgtt ttcggtaaat cttttatatg gttcttttat atatcgtggt gaattgcaaa
    26641 aaaatacaaa tgatgggtat ggagtgggta gctgtgtgca atattttatt tttacctttt
    26701 tgcctttata taatggtggt ggattttttt caaactcatt aagcatgatt ttatttaatg
    26761 tagatgtagg aattcttttt tttcgctcat cataaacttt tttcactact tccaaaactt
    26821 gcaaaagtct ctgtcgtttt attgcactag taaatataat aggtatatga tcttgtggag
    26881 cgagtttttc catcaacatt tttcgatatt cattagcagt tttagtatct ttgtctatta
    26941 aatcccattt atttacagct aaaactacgc tttttttatt atcattgata atgcttaaaa
    27001 tatttaaatc ttgagcacct atcccttcgg tagcatctag tactaatata catacatctg
    27061 cactttctat tgcccgtata ctacgagtga tagaatagaa ttctatatta tcatttattt
    27121 tacttttttt tcgtattcct gcagtatcta ctagataaaa tttatgatca aatttattgt
    27181 acacttcata aatagtatct cgagtagtac ctgctatagg tgttactata gacctctctg
    27241 aaccaagcaa agtatttaat atagtagatt tgcctacatt tggtttgcct actatagcta
    27301 tgcgtggata gtctaattca tcatctttca tttctggtaa aagttctact actacatcta
    27361 acatttctcc tgtgcctaag ccatttttgg cagaaatagg aaagattttg tcgaagccta
    27421 attgataaaa ttcggtatga taatgatata tagaactact atcagtttta tttgctatta
    27481 ataatactgg ctttctctga ggtcttaata aatttgctat atctatatct aatggagtga
    27541 taccatcatg agtatctacg acaaataata tcagatcagc ctcatcaatg gctaaatcta
    27601 cttgtttttt tatttcttgt tgaaaaatat catctgattt tataactaca ccgccagtat
    27661 ctattactgt aaattctacc ccattccaat agcatttacc ataaatccta tctcgcgtta
    27721 ctccacttgt aggatcttct atagccaatc tgctaccaat taatctatta aataatgtgc
    27781 ttttacctac attaggacga cctactatcg ctactatacc tctcattatc aattaaataa
    27841 taatattgtt ttaacttttg aattaaattt tatgcaaaat taataaattt cttttagact
    27901 actgatacat ctcattatct ttattgtttt caataaataa ttttatcttt tatattaata
    27961 atctcacttt acaattaatt tataataaag ataaaccaaa tcaatacaag ggagtattgc
    28021 aatgccatat acttttgcaa cataaatcct aggtaacatg aatgcttctt aaaatgtttg
    28081 aaaattgatg agatttaatt tgaacataat ttttaaatga attattttgc gagttatgag
    28141 tttattctaa atcttttttt ttaaaaaaaa aacaaacaaa tcagaattca gacaattcac
    28201 tcatttactt tttgataatt ttctttatcc atcactttaa aaactggaat agttcgcata
    28261 cgctgatcct tccagggtga attatcaaaa aaccacatta attgtgccca aggatcatta
    28321 gcaaattcag gttcttttaa ttttttttct cgaaaagcat cagcaatatt tttatcattt
    28381 aacatatttt gagctaaagt atctagaacg tatatttctc catattcttt tctttcaaac
    28441 atagaattga aatagcccat ttgcataaaa ccaactgtag attcaggttc taaaagatag
    28501 actaaacttt tccaagcttt ttgattaatt ggaataaaat aactacctct aggcaaatca
    28561 ataaatgtat cacgtaattg atatgaaaaa atagggctaa aagtaccttc aaaaattgca
    28621 ttatcaaatt ttaatttagt caaataataa tattgcactt ttatttttga gatttcagaa
    28681 agagtataag aagttacttg atgaagttca agtatttcta tcaaatgttc ccactctggc
    28741 cctagtaaat atccataggg caaaacgaca ttagttttta cctcaataga atcccaaaag
    28801 tctaaagcta cctccatcgg cttagtatta tcatatgtat aataagatat acctgtgaca
    28861 ggacttatgg ctttatgata ctcatatcct ttcaatattt ttttattttg atgttctgtg
    28921 agcttatacg ttatcggaaa agttttattt ggttttgcca ttgatagaga gtaattatct
    28981 tcatttaata aaagattttt taaaagattt ttattattac ttaaccattc taaaactata
    29041 agtaaagctt gatgagtagc cataaccctt tgtttataag attttaacat atgagtttct
    29101 agtaataatg ctggtttatt attcagaagt ggatagccat gagatagaat aggactataa
    29161 atagaagcag tcaacccact ctcaggacta aaccaatctc taaaagccac atattgtgta
    29221 gaaagaaagc cattattgtc catttcatct ataaaatatg taataaaatt atgttcaaaa
    29281 aaattattaa aatcagtact tacgaaagac ggtaattgca tatcaaaagt catcacatat
    29341 tgataatcag caccgtcagt ggtgtgacaa tctataaaaa aatctggatt ccaataataa
    29401 taaaatttta accaattttt catctcaata gagctagctt ttagataatc cctattcaga
    29461 tttagattat gtgcattagc tctccagcct tgaagctcag gaccattttg attgatacga
    29521 tgatagggac ttaataattt caatccatcc acattgaaag ctggaataaa aataatagta
    29581 acattattta gcaaattcat atatctagga ttagataata atgatctaat aagcataaat
    29641 cctccatcta ttccatcagg ttctccagca tgtattccag cctgtatcat cagagtcact
    29701 ttattttgct tagcaatttc tgttggatta tttgctttat ctttaaaaat aatcaacgcc
    29761 gctatagtgt cattctgctc agataaacct aaatatgtga aatgaatatt atcgctatat
    29821 tgtttagcta gttcatgcga aagcttcatt acttgctcat aagacaaact tttatcaggt
    29881 tcattgtcta taggtaaagt gaaaatttta ttgttttgac ctgataataa gctcgaaatt
    29941 attagtgtaa aaaatataaa tgctattttc atttcaattt ttttttacaa aaatacaaaa
    30001 atgtaatata agattttcaa atatatttta tttatcacga tgttgtttat ttactaataa
    30061 caaaattaat ggaaataaag gctcaatcat cattattgct acgattttaa ttatatttat
    30121 aaaaaaaact ttcatacttt aatattttaa aatatattac tagtaacata aggcccttga
    30181 tttgctctct atattttgct ccagtcacca ggtttgcctt gtcatgttag tgttttgata
    30241 tttagattta tttatacgat aaaccaaagt aattaaatta gtcttattta taaggtaata
    30301 gagtagggga gagaaatttt tttttaaaaa ttaattacat tttgtgtcag ttctgaaatt
    30361 gcaataggtg atttagtata cacacaatta cttattcgtc tataaatatt gagtttttat
    30421 cttaataaaa tataaaatca caacaactta attataacta atgatttcac ttattaatat
    30481 atctataaat ttcactattt ttgaaatcga tcatcatttt ttgaataaaa accaaaaaat
    30541 cattttcaaa ctctgaataa ttatctagta aaacttctac ggcactaggt aactcagaat
    30601 cgaagcttgt tttacctgac atccattcaa aaacattttg taagcccgaa atatccttgt
    30661 acatcaataa ccaattattt gtaatcatgt gatctacagc tttctgagct ttagtaggta
    30721 aaatgttata attattttca agtaaaatat agaactcatt aatagctatc aaaaaaggtt
    30781 tagtagcaaa ttgttcccaa tgatttgcca taaaatgatc atagataata tccaccgcta
    30841 ccggggcata atgttgtaat agaggattta aacgacggat agattttttt acactagagt
    30901 tactatctga gtaattatct atccattgat gtagttttat tcctttttgt atattttttg
    30961 tatatgattt aagtctatta aaacccctta tataatcagc tataaaatta cctaaaatga
    31021 cttcatcatc atcaggagca aaaaataaat gtactaaaat attcattttt acttattcac
    31081 aaacaaacat acataaatta tttcataatt tcaatacttc tcttaatgaa atcagtaagt
    31141 ttttcgccat cgagtaaatt ttgagatatc aatgccagat ctatcaattg ttcaactttt
    31201 ttattttttt cattattatc attataattt aaaatctctt cgatgagagg gtgagcagag
    31261 tttatgatca tagtgtattc tttttttaat ccttctaagt attccatacc accaagcgct
    31321 gccatttctc taaatctacg taatatttca ggttgcacca ttactacagg gagagattca
    31381 tctggcatat tacgaataga tattgagata tttgcatttt ttaaatcttt aggaaggagc
    31441 gattcaaata atttttgtaa ctcttgttct tgttgttcag atatttttgc tacatcagat
    31501 tttttatcct tttctattaa actatctaag gtatctgaat ctatacgttt aaaactaaga
    31561 tcttcatatt ctctgtctag aaaatctaca aaatgagaat ctaaaataga atttagtaaa
    31621 atcacagtat agcctttatc gattgcaggt tttataaatg aatgttgtga atcaatatct
    31681 gtagcgtaag gcacgacaat ttttttattt ttatctgttt gagtatcttt tattttttct
    31741 tttaactcat taagagtata aaaattatga ttgatatctt cgaataaagc gaaattaata
    31801 gctttttgtt taaaatcttg atgagtcatt atgccaaatc taacaaaatc tctgagagct
    31861 ggccacttat tttcgaaatc ttttctatct ttcaaaaaca tctcttctag ggtatcagct
    31921 acacgcttag taatatgatt ttttatttta cgaacattag catcagcttg cagagtactt
    31981 ctactgacat tgagaggtat atctggtgta tctattactc catgtagcaa catcagaaaa
    32041 tcggggacta tatcttctag attatcagtg acaaagactt gattacaata cagttgaatt
    32101 ttgttacgtt gaggttctat tgtattttta atctctggaa aatacaaaat accattaagt
    32161 gtaaatgggt aatctacatt tagatgaatc caaaaaagtg gtttttctaa tgaatatgga
    32221 taaagctctt cgtaaaaatt gagataatcc tcatctttta gctcatttat attttttttc
    32281 cataatggtt gagtattatt tattatacga ggtatatcta tgtattctgg tttttcaggt
    32341 gtacctatat ttttcttttc tgtaccgaat tgaataggat agggcaagaa acggcaatac
    32401 ttttttaata tgttcaaaat attatattca tcattatatt cattagcatc ctcactaaga
    32461 tgcaagatta ctgtagtacc gcggtcgggc aaatctacct cttcaatagt atattctgta
    32521 gtaccttcgc aaacccaatg tacacctttg gtttcgggta aataagattt tgaatatatt
    32581 tctactttgt cagctgccat aaaagcagag taaaatccta acccaaaatg accgattaga
    32641 tcttcgactt tttcttgatt ttttaaatat ttttcaacaa attcttgtgc accgctaaaa
    32701 gctatttgag taatatattt ttctacctct tctgtcgtca tgccgatacc tcgatctatt
    32761 actttaatga tttttttctt tggatcatgt tctactttta tggtaaggtc gcccaattcg
    32821 agttcagctt ttcgtagcga aactaattta gacaatttag ctgtagcatc tatagcatta
    32881 gatattaact cacgcaaaaa tatatctgta tcagtgtata aatatttttt gatgatagga
    32941 aaaatatctt cactttttac atctatgaat ccttttctca taataatttt ttcatttatg
    33001 ctatcaaaat acattcctaa tgtaataatt agtgccaaaa tgacagaaag gaataattat
    33061 aaataaataa ttttataaat atctttaaaa aaaaataatg atagaataaa tcaaaaattt
    33121 tcaataaaaa atattaagaa ataattgtaa ttataataat aataaaacat aaaataaatt
    33181 ttaacagaaa aatattatgt tgccacaatg aaataataat tctaattttt ttcctgttaa
    33241 ttaaaagttc tatatgtttt aaaaaaaata acacatgaaa tactcattta gaataaactc
    33301 aatagtacag taaatatatg ttatatagat tatatctatc atattttagt tttgtaattt
    33361 tgcatatatt cttttgtcct ataatttata ttatgttaaa tagaatttta aataagttta
    33421 aaagttaata tctagcactt tgctacctgg cccttaatgg tgaatcactt atctattttt
    33481 aggccaaagg ccatccccat tcctttgtct aaccctaagc taaaggtcga tgtttgtgtg
    33541 ttaaaagaga gatatcacca ttacatttta ttaacattat tgttatattt gtaaaatata
    33601 aaataatttt aatgctagta aatgttgctt tgccattgcc aatagataaa acttttattt
    33661 atcgtgtacc atcagatata gagcatgata ttcaatgggg aattcgagta atagtgccat
    33721 tcggtgctaa aaaattatat gctggtgtag taatagacat tctttcagat gaacaattgc
    33781 aatacataga tgtatttacc aataatatag atgaaatcaa atatatttta gaagtattag
    33841 atgataaacc aataataaat aatgagcaat gggaactcta taaatgggta gctgactatt
    33901 atctagcaaa tatcggagat gtattgaata atgccctacc tagtggacta aagatacaat
    33961 ctgaaactaa atatcaacta catccagata tagatctctc ctctatcaag actgaagagg
    34021 aaaatttaat ttatgaaatt ttgctagaac gagaatttat cacaaatgag gatatacaaa
    34081 aattattagg tataaaaaat atacaaagat ttatcaaatc ttgcattgat aaaaatataa
    34141 tatatcctac tgaagaatgc gaggagaaat atattcccaa aaatgttaaa tttattaatc
    34201 tgagtgaaga gtttctcgat tcaaaagatt tcaatgacaa aataaataaa ttagaaagaa
    34261 gggctcctaa acagatagat gttattcttt atctacttca gaagcgctcg cagggacaga
    34321 ctaaaatacc caaagctgag ctaacaaata agttttctat ctcagctata caatcattaa
    34381 taaaaaaagg atatataata gaaacttctg aaactatatc taggattgaa aacaaatttg
    34441 ctactacaaa tgagattgag gaaattatat tatcagatat acaaaaatca gcttatcaag
    34501 agataaaaaa ttacataaac aaaaacaagc atgtactttt gtatggtgta ccaggaagtg
    34561 gtaaaacaga gatatattcc tcattgatac aggaatatct agatgcgggc aagcaagtgt
    34621 tgtatctttt acccgaaatt agccttacaa cttatttagt taataggtta cagaaatatt
    34681 ttggggataa aatattagtt tatcatagta aattcaattt gcatgaacgt gtagaaattt
    34741 ggaataaact attggatcta tgggaaaaag acgatccatc atgtatagtt gtaggaccgc
    34801 gctcagcatt atttttgcca ttttacaatt taggtttggt agtagtagat gaagagcatg
    34861 atccatctta taaacaacaa gatttaaatc caagatatca tgctcgagat atgagttata
    34921 tattagcaaa caatcatcat gctaatctaa ttatgggaag tgctacaccg agtttagaaa
    34981 gttggtataa tgctaaaatt aaaaagatag gtttcgtaga aataaaagaa agatattctg
    35041 gcatcatacc gccattgata gaaattgtgg atatgaaatc tatgatgcag caaggcaaag
    35101 tgagagggca cttcagccat ccattattaa aagctattga cgatgaagta aaggcaggaa
    35161 gacaagtttt gctatttcaa aatcgtcgtg gctactcatc gtggatggaa tgcaaaaatt
    35221 gtggatatat ttttccttgc ccacattgtg acgttaatct cacctatcac aaaagcgaaa
    35281 atgtgctaaa atgccactac tgcggttata cgattgatat acctactttt tgtccaaagt
    35341 gtaaatctaa taagttgata ttcaaaggat ttggaacaga aagagtagaa accgagttgc
    35401 aaaaatattt tcctgattat cgaattgata gattagattt agataccact agacgaaagc
    35461 atgctttcaa ggagataatt caaagatttg agcgacgtga aactcaaata ctcgtgggta
    35521 cacaaatgat atcaaaagga ttagactttt ctaatattca gttagttgga gtcttgaatg
    35581 cagataattt acttgcctac cctaattttc gcgtttatga gcatgctctg caactgctga
    35641 tgcaggtagc tggtagagct ggacgtagag aggtacaggg tagagtttat attcagactt
    35701 atgctctcaa taatcctatc ctaccctatt tagtaaataa taattatgaa gattttgccg
    35761 aaaatgaatt aaaagataga tggcaattca aatacccacc atattataga ttaatacaaa
    35821 tacaattgag acacaaaaaa gaagatattc taaaaatagc tgccgatgct ctaacagaaa
    35881 aaatacgaaa tatcatagat gccgaatatg tgaaaggacc tgtagaacca gtaatttcaa
    35941 aaatcagaga ttattattat cgtcagattt tgataaaaat accacgtgat aacttactaa
    36001 aagacaaaaa acaaaaaata aatgaggtag tattctatac tcttaaaaga aataaagcgt
    36061 ggcgaggagt gcttgtggat atagatgtag atcctgttta gtgacttggg agaattggag
    36121 actgggagac tgggatgaca aggagactga aggtgtgatt tagtggttaa atataaataa
    36181 taatcatact taatttgtct aaaataaaaa atatctattt tttactactt tcttttttgt
    36241 gttttttgtt aatttatata taattaatat taattaatat aaaaaatata tttatttgtt
    36301 aataatactt tttaccaaat tagatttggt aaaaagtatt attgattatg cattattaaa
    36361 taatttattt taattataat tacagctctc ttaatgttta atttataata attttgctat
    36421 tatattcttt gttattactt aaaatgttta aaatataaat tcctggcatt atattttcta
    36481 gtgaaatgat attatttgta ttgttattaa tagaatatac tatagagcca tttatattat
    36541 atatattgat gttagaaggt atctgatcgt agaaatagat tttattatta tttatgaaat
    36601 aattaaattc actattatag aaatcatctg tataaacgtt atcgggatga tacataaaga
    36661 ttacttcacc caggttagga tcatcatttt tatgataaat gacctcttcg atacttacag
    36721 aatcttgtac ttctacattc catgtattac cttgagcata gatagtattt ggagaattgt
    36781 tgtacaaatc atacagtata ccattattac cattatttac aaaaacattt agtccaggat
    36841 taaattcact atcagtagag tctacacata atttaccaat atttacattg ccaccgctca
    36901 ctattgtaat accccataaa ttttcttcga tatgattacc ggtaatcatt gtattttgta
    36961 tattattttg atcatagata ctaatgccac taccaccagt cataggatca ggatcatatt
    37021 tattgtcaat tatttgatta tttgatatca atgcattcat ctttccatta gtcgtgatac
    37081 cataacggca attattaacg aaattatcac taataattac attattgact cctgaaactc
    37141 ccaacaaatt agctactcct ataccaccaa cttttgatct ttgtgctcct attattacat
    37201 ttcctacaat ctctattgta ccatctccgg atggtcccat atttatttga ggtctattag
    37261 tattgttagt gttattatca tatagataat tatatttaaa agataaagaa caagatacat
    37321 tagatccact agctactgct gaactttgat tacttataaa tatacagttt tcgataacat
    37381 tattaccatt tagtaagtta atagccgaag agctaagttt ggtatttact agagtaaatg
    37441 tacaatttct tatctctata cctatagggc tactagattt aataccagca tattcaaatc
    37501 tcatattttg tattgtagca cgagaattgt ctccttttat ataaatgcct ttagggtttg
    37561 cagaagcatc tgatctagta actaaagcag tatctgaagg agtaaaatca gcataacctt
    37621 caatattaat tgtaacgcca ttacttaata taatggtatc attattattg atttttaaaa
    37681 tgtctccatt agatatagta aagctagtat taacaataaa tgaatttgaa aatagttttt
    37741 caacacctga agtaggtata gtgcttaatg agtcaaaagt atagacagtg ccatctccat
    37801 ctgaaataaa ttctagagtc tcctttgcat ttttattgtc ataattttcg taataaacat
    37861 cttgaatgag gtttacagtg attattgcat tattatcttt aaagatttca gtatgattta
    37921 ctttttgtgc aaatagattt attgacatca ccattatgag tgtcgtacat aaatttttta
    37981 ctttcataac tttgtttttg gcaaaattac aaaagtcttt atattttttc aagtttattt
    38041 tgtaaaaatt taaatccttt taataaaaat tagaaataat aatttttttt ttgatcttta
    38101 agtactaaat caatttttta atatattatg tattaaaaaa tgtttctaat ttgttgtata
    38161 ttgatactca aaacaattct tctattgctt ttagtaatat tttttctgca tcatctaaag
    38221 agccataaaa atcggctcca gaggcatttt tgtgtccacc gccattaaaa tatcgttgag
    38281 ctaaatatct cacgtcataa ttcgttttac tacgcaaact aatctttatt cgatcctttc
    38341 tctcggagat caaagctgaa atatttactt tttctatact cagaggcata ctcactatac
    38401 cttcggtata tcctggttca taattaaact tttgtagttc tttttgtgtc aaaataatat
    38461 aagctgcacc tttattttca ataaaattaa gttttttgta aatactataa cctaaaagtt
    38521 taaatctatc ataagtataa gaattataaa tgaggttatt aattttatta acattcactc
    38581 ccattttaat cagattagtc attatttcat atggatgtga atgatcacag caatgaaaaa
    38641 atttgttagt atcagtataa atgccagtat aaatacactc agctatctct ttatcaaaca
    38701 tactaggatt tatgaaatct ataatatcat agataatctc agaagtcgaa gaaaccttag
    38761 tatcagatat caataaattc cattcatcct caggaggatt taaatgatga tctatacaaa
    38821 tactaaaagc tttaatactt tgtgtcgaac acgacatttt taaattgcgg gttaaggaat
    38881 tgaaatctgt agcaataaat atatcagaat cagatatcaa agaatcacat aactcatgat
    38941 cattatcaaa tatgtagcta tcatcgatac ctggcatcca tttgtaaaat gaagggatag
    39001 catcaggtat aatagtattg acgttatgtc caattttttt taggaaacga ctaagggcta
    39061 aggatgatcc taaagcgtcg ccatcggggt ttttatgagt agttactact attttgtgtt
    39121 tcttcttact cataaaaatt gatttcaatt ttaaaagttt agcattattt ttcatattaa
    39181 aaaaatattt taatttagca tgcaaaattt tgcaaattta aaaaatatat tattatggca
    39241 atacaacgta ctcttgcaat tattaaacct acagcagtgc aaaatggtca tacaggtgct
    39301 atcctacata ccatttgtgc aaatggtttc aagcttaggg ctctaaaaat gatgaaaatg
    39361 actaaaacat tagcctctac tttttatcaa gaacataaag atatggaatt tttcaatgaa
    39421 ttagtagagt ttatgagctc tgggcctatt gtggcaacag ttttagaaaa agaaaatgcc
    39481 atagaagatt tcagaaatct aatgggaaca actgatcctg ctacttctaa acctggtact
    39541 cttagatatc aatatggtac tagtatcaga gctaatgcta ttcatggaag cgaaaacgaa
    39601 gaaagtgcta aaagagagat agagttgctt tttactaaaa aagaaatatt ttaatctaac
    39661 ttgacatttg tatattttta gtagctttag tgtttgaata aatgattaga gtgttataaa
    39721 aatattgtga ctttataata aatatctaat tatattaagt tgcatttact tgatatattt
    39781 taactttcag gataaaaata tttataggtt ccatcagaaa agaaccatac tatactaatg
    39841 atatcttttt gtctgattgg tttcatttca gcttttgaga tatcatcctc ttgaatatct
    39901 gctatagggc tctcagtttg ctcggtagta gatggtggta ctgtttcttt tgtttgctca
    39961 tttattgaca aatcatctat tatcttatcg tctttaattt tttgtttagt tttttcagga
    40021 atgatcaaat ctaattctcc tgtttgtaat tgatctttac taaatatttt cttcttaaac
    40081 atatcccctt tgtcaaataa taaccattct gaactaatat caggaaaatt ctcgagtatt
    40141 ttcataacta cttctagaga tggtttatta cggccattta gaatatgaga taaacgtgaa
    40201 cgatcgattt ctagagtatc tgcaaattct gatggagtga gtttataata ttccattatt
    40261 ttaataattc tatctaacat atttttttat ttacaaaatt aaattatttt tttaaattga
    40321 attattttaa tcaatttaaa aatgtataat tataagttga ttaatgtaaa cattaaagta
    40381 aaggtttaat actttaactt atttgattga tatacagcta attactaatt ataataatct
    40441 attttgaaat aaaataattt ttgtttaata ttttaaagta ttacttgaac atatcaatat
    40501 taatatattg ataatcaatt atttaaaggc aattataaac gagatataag catatttata
    40561 atactttata ataatattag ataatttttg cttgatttac tattgtaaat atatttactt
    40621 ttgtaaatta gtaataaatt gattaatgta aactgattat tttataaaaa ttaattagta
    40681 tctaataaat aatttacata tgttaatgtt ttggtattct tctaaactat ttattgttgt
    40741 ttatatttta tcgatttaat tatttgatga tgcatgttga gatgtaataa aggtattaaa
    40801 caaactattg aatgactaaa tgaaatgata acagtaaaaa attgttaaaa ctaacaaaca
    40861 aatgttaaaa tttgatttaa atgtttaatt attttcagtt aaaccttaaa ttgaaaataa
    40921 ttaattaatg cataatgcaa atgctatgac aagtaacaag ctaaatagag aagtatattt
    40981 caaataagga tccaagtctt taccactttt atgaaaaatt ttaaaaaaca ttataaaaga
    41041 caataaaaag acaaatgtat ataaatatct atgccaagga aatgacgcat aaataatact
    41101 ataagaaata aagcataaac acattgagca tgtgagaata aggtgataaa tctttgcatt
    41161 tttcaatcca agcttgacag caatagtaat tttgccagaa gcgaggtcat tgtcaatatc
    41221 tcgcatatta ttaatattca gaatcattgt actagtcaat cccataccaa tggctggtag
    41281 tacaggttga aaatcaagag aatgcacatg taaaaaataa gtaccaataa cagcaacagg
    41341 accaaaaaat aaaaaagcga agaaatcgcc ccacccttta tatccataag catgtttgct
    41401 caaggtataa aaaactgccg ctaatatgca aagaagtcca ataataagca taatgattgt
    41461 cgatttccat ccaataaact tagtagagta attaacaagt gttaatccga taatagctac
    41521 ccatattata accaatccta cagctttttt catttgcttt acagaaatat atccactctg
    41581 aagggtgcga atgggaccct tacgatctcc agtagtatct gtaccttttt gataatctcc
    41641 caaatcattt gccaagttag acaagatttg taatgataca gctaaaatca gtgtcagaaa
    41701 gaatatttta ccataaaaca ttccttcgtg ccaggctaga ctgcttccta atatcacagt
    41761 tgctatggcc aaaaaaagtg ttcgtaatct aaaagctgat atccaatttt ttatggtggg
    41821 catattcatt tattaaaaaa agattcatta tgttgcgatt attacagact gttttaatat
    41881 atattatcaa aaaagcttcc ttgtttgcct ttatttgtca ttttaatcat tcattttgcc
    41941 tttataatca ttgaagttgt agattttttc gaagggttta cgttttttct ctggaaattc
    42001 atcattttta ggataaccta tagtgataat cagaacaggc ctactagatg caggaatatg
    42061 aagtaatgac cgtacttttt tctcattaaa ccaaccaaca atacaagtac caagccctaa
    42121 ttctgtcgct tgtaaacaga aatgagccgt agcaatgcca atatctatca aattataagg
    42181 cttatctttg attactgaac ctaaggtaga tgatatattt ggtctttcca ttatcacaac
    42241 tacatgaact ggagcctgtt tagtaaaatg attaataggt actatgccct tagatgtagc
    42301 atcagcaatt tgatttttaa gattaggctc attaacaaca ataaatttcc acggttgagc
    42361 attacaggca gatggtgcta aatgaacagc ttgtagacat tttattattt ttttatcttc
    42421 tactggctta tcactgtatg atctcacact ataacgacgt ttgaccagtt ctataaaaga
    42481 agtattttca ttttccataa taacaatttt tttaaatcaa actaaatatg atattaaaat
    42541 taaatgtttt tgtaaacaat caatctattt aataattttc atacaaaatt aagatttatt
    42601 atattgtttt tttcttttta gcatgaaatt atcattattt tttcaatttt tgataatatt
    42661 ttaatatgtc gttataatga gtaatcggta aaattatgaa ttataattaa ttgttttatt
    42721 aaatagacat acaaaaaaga tggaattaaa agggttataa atggcacttt gtggactttg
    42781 tgtaataact tggtgccctt tgtggtaaaa aaataaccta aataattaaa aaatagttat
    42841 ttttttgtaa caatctaaaa tgtttttacc cacaataaac gttatagaac ctaattttta
    42901 tatttaatta atatatagga ttgtacaaca cacatatcaa taatgtatac taaaacaaaa
    42961 ttgtgatagt aaaattgtaa tagtattatt atttttctat aaaaaaatca gcctctctcc
    43021 cactctatca gacattttca atggtttctg cttttttttc taagatttct tcgagatatt
    43081 ttctcgccat tttgaaatta cgtgtaggtt ctatgctgag tatgatacga tgtgtatctt
    43141 gtttcatatt agtattttta ggatgatttt gtatgaaatc taatatattt ggtaattttt
    43201 gaataatttt atctttgtga tagtcttttg aggagaaata aagagtaaga atatttttgt
    43261 ataaagtgat tttttctata tacagctgct gagccaacca tttgagttta atagcatcta
    43321 ttaattcgat tgtttctgga ggtaatggtc cgaagcgatc tatcatcatc tctatgatat
    43381 catttagttg attctcacta ttagattcat taagttctct atacaattta atgcgttcga
    43441 tgctagtagg cgcataaaat tcaggaataa aaatacttcg atcagtatca atagtaatct
    43501 ccgaagaggg tatattaagt tcattatcag ttataggcat attcagttct tgacgtagtt
    43561 ctattagagt ctcatcgagt attttttgat acatttcata acctacatct tctatgaaac
    43621 cactctgctc agcaccaaag atagaaccag ctccacgaat ttctaaatct tttatagcta
    43681 ccgaaaagcc agatcctaga tcgctaaact cctcaatagt ttttaatctc ttttgtgcta
    43741 tcgaagagag aaacctagta ggaggtatca gtatatagca aaaagctttt tggtttctac
    43801 gacctactct accacgcaat tgatgtaaat cactgagtcc aaaattttgt ccgtcattga
    43861 taaaaatagt attagccata ggcatgtcta tacctgactc tataatggta gtagatatca
    43921 gcacatctat tttgcctttt agaaaagcca tagtaacggt ttctatttca tcagatttca
    43981 tgcgcccatg agctataccg actctagctt caggtactaa agtttcaatt aaattagcta
    44041 tttcagttat agtttctata ttgttatgaa taaaaaaaac ttgacctcct ctctctatct
    44101 cattttcaat tgcttttttt ataaatttat gttcaaaagt tcttacttca gtatgtattg
    44161 gtttgcgatt aggaggtgga gttcgtagca cactgatatc tctagccccc atcagagaca
    44221 tttgtagagt acggggtata ggagtagcag agaggtataa tgtatctaca ttagttttta
    44281 attgtcttat tttttcttta gctgctacac caaatttctg ctcttcgtct ataattaaaa
    44341 gtcctaaatc gttaaatttc acatctttgc ttaaaagtct atgagttcct ataattattt
    44401 caattttccc ttctgctaat tgtcttaaaa tttctttttg ttctttacta gtacgaaaac
    44461 gatttatata ttctactgtc acaggaaatt cttttaatct ttcagaaaat gttaaataat
    44521 gttgaaaagc aagtatagtg gtaggtacaa gtataactgt ttgtttgcca tccaaagcgg
    44581 ctttgaatgc agctctaacg gcaatctcag ttttaccaaa tcctacatca ccacagatca
    44641 gtctatccat aggataatca ctctccatat ctttttttac ttcttcgata gcttttagtt
    44701 gatctggagt atcttcatat tcgaagctag actctaactc tatctgtaag tagttatctt
    44761 tttgaaaagc gaaacctttc atagctttac gctgtgcata cagtgttatc agttcacgag
    44821 caatatcttt gatgtgtttt tttagcttat tttttttgtt ttgccaagca atactaccta
    44881 gcttatgtaa tgtgggtggc ttagcatcag aacaaatgta tttagtgatt ttgtgcatag
    44941 actgtatatt tagatatagc acgtcatcat ctttaaacat tagctttatg acttcttgtt
    45001 tatttccatt tatttctaat tgttctagtc ctgcgaagcg cccaatacca tattgtatgt
    45061 gtactatgaa gtcgccgggt tgtagctcca ttagctcttc tatattagta acttgttttt
    45121 tatgtatttt attaagtaat cgaggtttta aatatttttt gaataattga tgttctggta
    45181 caactattat cttagtttgg ttatctatga atccttcatt aaaaactgtt ggtaaatatt
    45241 ttatataggc taatacttcg gtatcatttt ctatgtccat atcttctatt atagcctgca
    45301 ttcgttccca tgctggataa tgtggactag tgaaatatat actataacct tcataaattt
    45361 tttctttaca gaaaagatga taatccttta aaattttagt aaaaatcggc aatggtttta
    45421 catcaaattg tattaattta tcataatagt ttacatttgt attagagaaa tttacatttg
    45481 taaactcatt gatttgtgtt ataaattctt gataatcaat gaattcttta ttgatttcat
    45541 cggataattg atttaaaaaa aattctatat tattattagt tagatcaaaa ttactagtcc
    45601 atattataga atttatattt aaaagttgaa aaatagaaac tttagtttga ttaagctcgt
    45661 ccatggaacc taaaatatct atacacgtaa gctcttggat actcatttga gagactacat
    45721 cgatgagttt cacatgagac agtgtctcat ctttaaacaa taaacgatag ggagcattag
    45781 cattgaatgg ataaatatcg caaatagctc ctcgcagtgc aaactgtcct ggctcataca
    45841 catagtctac ctctacatat ccataatata atagaagctc aatcaaaaaa ttcagatcca
    45901 ctctatcccc tatcttgagc tttgttttat ttttaataat attttcagat ttgggtattt
    45961 tttcaactat agcttcggga taggtgataa caaggctcac tatatcatca tcagcaaaaa
    46021 gctctgaaca ctgcaatcgc atagcttcat tagagctatt aatagaaaaa gtatcgaaat
    46081 ttttgtaact cgatggcaaa tattgtaaat aaattttgtc atttaattgt aattgctcta
    46141 tccaataatc taagttatta tatatctgta aagcatcatc ctcattattg gctatataaa
    46201 tacctttatt agctttagtt tttctataaa aaagtaaaag tataatagaa gatagagcac
    46261 ctgctacctg actgatttga atatttttat atgttttaat agatttttgc aattcagtaa
    46321 aagcaggatg cttagattct ttatttaaaa aaaatcctct attatattaa tcatcttaca
    46381 aaattattga aaaatagaga aataacaaaa caataaaaag gcgaatttat cagaaaaaag
    46441 aaaaactaac aaaagaaaag tctccgctag ttttaacacc cctgtctctg tgagacatcc
    46501 cctctacaag aggggaatac gatgtgattc cccttctccg aaggggtgtc aggctttgcc
    46561 tgacggggta gttaaaaaaa ctacgctggc tagccctacc ccgcttgctt gttttattgc
    46621 aaatcatttc ccaatgcgga tgggacacat ctaaagatgt gtataaagag aactaatagt
    46681 tttgattgga aacggagagc tctctatcat agtatctcgc tccgaagtat gtgtagctgg
    46741 tttcgttgtc tagctccttg gcagtgttca taaaaatatt ttttgttagg taaaattaat
    46801 gaatattatt gtttttctct tt
//
LOCUS       DAOPRN010000009        43330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_663_length_43330_cov_137.273252, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000009 DAOPRN010000000
VERSION     DAOPRN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 43330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43330
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_663_length_43330_cov_137.273252"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1033)
                     /locus_tag="PKI65_01970"
     CDS             complement(35..1033)
                     /locus_tag="PKI65_01970"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77240.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELNRARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSPEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRKNVIKHKEIVTSNRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYTMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNITYVD"
     gene            1283..2911
                     /gene="lnt"
                     /locus_tag="PKI65_01975"
     CDS             1283..2911
                     /gene="lnt"
                     /locus_tag="PKI65_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOB77241.1"
                     /translation="MVKQKNYTKIYLLLASITGGGLLSLSWPLRGFPLLSLISLVPFL
                     WILDYIENHRTDFVGGAVFRYTYLGFLLWNAATSYWIWYATAVGSIFVILVNAALMSF
                     SLQLAFNLKKWLGSKSKIGIIYIILWLCFEYLHFNWELSWPWLSLGNVFATMPAIVQW
                     YEFTGILGGTLWIWLVNIFIYYIILSIYNKQLKERVFYIYLIILFFLLAIPLTYSLFR
                     YNNINLKNSDEVNVLVLQPNLDPYKEEFTISPQENITRIKNLIDEINAEKVDFFLAPE
                     SNIQEGIWENNFDDSYSINEIKKILKEKYPNALYITGASTYKIFEDGESLTSTARYNK
                     YHDFYFDAYNSVLFIDTSGVVGIYHKIKLVAGVEQMPYKKIIKPIEKLAINLGGTTGS
                     LGKDSVPYVHPHYKANIGVAICYESVFGEFISDFVKRGANVLFVITNDGWWGNTAGHR
                     QHFSYASLRAIETRREIARSANTGISCVINKRGDVLKSTNYWEKTAFLTTMSLWNDET
                     IYTKHGDYIGRIAVFSLCLIILIDIIKLIIKKIH"
     gene            complement(3046..4020)
                     /locus_tag="PKI65_01980"
     CDS             complement(3046..4020)
                     /locus_tag="PKI65_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOB77242.1"
                     /translation="MKKILVIGATGQIGSELTPALKKRYGEENIVLAYYYPPLPKELA
                     KKEFSVQFDVRDIDKFKQACIDYNIDTIINLAAILSAKGEQEPLKAWDTNINGLINTL
                     EIANELKLERVFVPSSIAVFGPTTPRINTPQDTILQPTTMYGITKVSGELLGNYYFMK
                     FGVDIRGIRYPGIISSMTLPGGGTTDYAVEIFYEAVKNKYYNCFLKADTRLPMMYMAD
                     CIKATIMLLEADINNLKHHSGFNVTAMSFTPEELANEIKKYIPDFKIDYNPDFRQAIA
                     DSWPQSIDDSCAREEWGWQPDYDLAKMTKEMLIAISKKLQIPISGLTD"
     gene            4131..4904
                     /locus_tag="PKI65_01985"
     CDS             4131..4904
                     /locus_tag="PKI65_01985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOB77243.1"
                     /translation="MKRVDIKKSKGVALITGASSGIGEAFAKELAAMNHDITIVARRQ
                     DKLEQLSAELKTKHNVNINIIPADLATETGIEKIEEYISCTKDLDLLINCAGFGLRGD
                     FHNLDKENISQIIDVHLKAPSRFCKAAIPVMRDRGYGYIINVSSLAAFFPFKGSAIYV
                     ATKIYLVYLSKAIQKEVRRYNIKIQALCPGVTNTGFHYTEAFKDFDRSTVPKNLWMQP
                     DVIAKKSINALKNRKVVYIPGFKNKVMGVLTQIGNLIFP"
     gene            complement(4861..6330)
                     /locus_tag="PKI65_01990"
     CDS             complement(4861..6330)
                     /locus_tag="PKI65_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOB77244.1"
                     /translation="MNPTLLLVIFLSYVFMLFVAAYLTSRKANNESYFLSNRKAPWYL
                     VAYGMIGTSLTGVTFVSVPGLVGNNGFTYLVVVLGFVIGYIVIAFVLLPIYYNNNVTS
                     IYEYLKNRFSIQAQKSGSWLFIISRSIGASLRMFIVIVILQHFVLSNFGIPFWFSTII
                     FLLIIHGYTLVGGVKTIVWTDTLQTTFMIASLILTIYFIAQFMYIDVNELLNQVFHSE
                     YFYIIDKDPSSKNFWAKLLLSGMFITISMTGLDQEMMQKNLSISTLKNSQKNILIFSI
                     ILAIVDLIFLILGGSLYLYADFKDIAILQTTDYLYPTISFYYMNEMVGIIFFIGLIAA
                     SFSGTDAALTALTTSITIDLLDLPKKIKSNQKLSKYRMLIQWCVSAGMFILIMLFYMV
                     NNNAILDTLYTIAGYTYGPLLGLFTFGILTKANIDGGSIWQVVLFSPLCSFAIMKILK
                     TNLQFMVGWEVLVLNALITFILLWIIREKSNCQSELVLP"
     gene            6907..7638
                     /locus_tag="PKI65_01995"
     CDS             6907..7638
                     /locus_tag="PKI65_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOB77245.1"
                     /translation="MNTLLLSAGLGTRLRPLTNEIPKALIAVADIPIIKRNLDKLASY
                     NVKRVVINVHYLADKIMYYLSNLHYDNMEIIISDERSQLLDTGGALLKAKPLFLKNEP
                     ILIHNVDILSGTDFDKLMKYKEKNKIDALLVVQNRNNLRKLIFDKNDNLSGWINYDSL
                     DKQLTNVNEKFTKEYSFSGISLISYDLLDDIPFDGVFSIITLFLYWAKRYKVSYYEDN
                     DIYWFDIGTFDTLQQANEYFKKIDR"
     gene            complement(7646..8236)
                     /gene="coaE"
                     /locus_tag="PKI65_02000"
     CDS             complement(7646..8236)
                     /gene="coaE"
                     /locus_tag="PKI65_02000"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOB77246.1"
                     /translation="MDKYIIGITGNIGSGKSTFARWFADYNFYVIDMDLYAKNLYFNY
                     SFRRKATDILGFDPIYNDRLRTQDIADKIYSDENKFKQLTELFLEFLPPLVDNTIKNL
                     NDKYIVIDGALLFEYSLDKFCNLTICIYAPLILRYKRIKKYRNKKISKKRFLQIDKHQ
                     LSPDIKCKMADICVKNEGNIEELKNNFLKILSSIDI"
     gene            complement(8382..9668)
                     /locus_tag="PKI65_02005"
     CDS             complement(8382..9668)
                     /locus_tag="PKI65_02005"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOB77247.1"
                     /translation="MKKFIFCLFIFVLILFSCDQDEQIGLEIHPPSDRINVNVDTFLL
                     TAYTLKDDSLLSSRGRYNIAGYLRDPIFGLTTSSFCSQLKITKSNIIFGEDAVPDSIN
                     LYLKLNNIYAKSRKNDVMTFNVYELGEPLYYDSTYYSNRPIKKGNLIGKITFNPSQID
                     SILIDSVKAAPTLKIPLSLNFAKKIIDASFEGKLVNNQTFQKHFYGIVVEPVVENNQG
                     NLIVWDLTNILSNITLYYHTSSLSKVKEVFQMDATLQWYSYFEHDYTFASYNLLSQIN
                     GDTSLGKQILFLQPMAGTKVFIRFPDLKSEFKGKNVNINMAELIIPTSYDPTAKEFPI
                     PNQLTLAKIVDSTGKTAYLEDYIFSESLFDGYYYNQKKYYRFRLPRYTQNLLLNKNEN
                     LGLYLRVAGAIINPNRVILKGSDNGMKLVITYSYVN"
     gene            9926..10291
                     /locus_tag="PKI65_02010"
     CDS             9926..10291
                     /locus_tag="PKI65_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOB77248.1"
                     /translation="MFKNTNKEIGDLGEKIAGKYLISKGYEIVGQNIRWKKYEIDLII
                     KKDDWLIFVEVKTRKMGAYIPAIESINKNKLQAMSNFADNYIKEIDWNGNIRFDAVII
                     SYSYLTPPIIKHIENLSIE"
     gene            10515..12386
                     /locus_tag="PKI65_02015"
     CDS             10515..12386
                     /locus_tag="PKI65_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOB77249.1"
                     /translation="MQYKSRPIPRSIILLADILIVAFTYIFSYFLRFNFKIPIAEQNT
                     LYIGLFVIVVVKLIYFLSLGTYKHLIRFVSANDAIRMFFSLVFSVITISIINLLLYNI
                     AHIPYIVPFSILIIDFVFSVFFLLGYRLAIKVLFFEFFNGQREKSNVIIYGAGRNGSL
                     VKQVLERDAGTKYKILAFIDEDHYKQGKQLDGIPIYNPERLEEFLKSNTVAHLIISIP
                     NISPNKKAYLTELCLKYKTKVLVVPPVSKWINGELSFKQIKKIRLEDLLQREEINIDE
                     SDVYKTLYNRIILITGAAGSIGSELVRQCIRFNPFKIICFDIAETPMHNLKLNLENEF
                     TYEQLEKVQFILGDITNKYDVEQIFNEYKPQIIFHAAAYKHVPILEYFPKKAIEVNVY
                     GTYLVANAAIKYNVEKFIFISTDKAVNPSNVMGASKRLAELLVKYLNDTSHTHFITTR
                     FGNVIGSNGSVFEIFKQQIESGGPITVTHPDVTRFFMTIPEACRLVLEAASIGRGGEI
                     FLFNMGERIKILDLAKTLIQLSGLTLGKDIQIKFSGLRPGEKMTEELLTDYSRDIETK
                     NKNVLISSEQIIDLDDKVKNILFLINNYQKISNNEIVENIQNIIPEFNSMNSLYSRL"
     gene            12416..16099
                     /gene="purL"
                     /locus_tag="PKI65_02020"
                     /gene_synonym="purI"
     CDS             12416..16099
                     /gene="purL"
                     /locus_tag="PKI65_02020"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOB77250.1"
                     /translation="MKIYFFYSPDNQGYIAVESTRLSLKNIDKLLWLFGDDAFLLNVD
                     TLVGEFICTFPEMITPWCTNAVEIVHNIGISSIKRMEKLIPYDKYKSTIYDPMLHTII
                     SDPSQNLFKIKRKKEPIKYIDDIEKYNYEAGLALSKYEIDYLKYISETIGRPLTDCEV
                     YGFAQVNSEHCRHKIFNGVFVIDNIEQKETLFDLIKATTRANHGKVISAYSDNVAFIE
                     AEKSKIFTPVSGDMPSSYIEYEEELVYSLKAETHNFPTTVEPFYGAATGSGGEIRDRM
                     AGGIGSIPLIGTAVYMVADTRNFIEEKNLFGQSKGRFLYHNPVDTLIKASNGASDFGN
                     KFGQPLICGSLYTYEQMIDDQLIAYDKVIMLAGGIGYALKKYAYKKTVRPGQTVVMMG
                     GDNYRIGMGGGAVSSVATGKYNNSVERNAIQRANPEMQKRVFNVIRALAENHLNPIIS
                     IHDHGAGGHLNCFAELLDPIGGEINIDALPLGDPSLSDKEIICNESQERMGLVVDNSN
                     YEKIEKIALRECAPIYKVGKIYASNSICFVGNDNRKPFDLKFEYLFGKTPKKIIRDYN
                     KKYEFHNPKYNLNEFDTYLEKVLTNDAVACKDWLTNKVDRSVTGKVALQQTCGKIQLP
                     LNNLAITALDYTSNTGIAISIGHAPNVGIIDPAKGSRLSVAEALTNIVWVPLKDGLEG
                     ISLSANWMWPSGLPGEDARLYEAVKSLSDFCIKLKINIPTGKDSLSLSQNYPDGTVVK
                     GPGTVVITASALTNDFTKVVTPDIKNVENSSLIFINFSKMPPSLGGSIFYQELEQIGN
                     NTPDIVDVEYFKRAFIVLQNLINQNLVLAGHDVSAGGLITTLLEMSFANPDIGLDINL
                     ASIPYDDIIAILFAENPGVVIQVANVKEVINQLYQAEMQYYPIGTVIDKRELKIIKYQ
                     TYEHFDIDKWRDIWYKKSYLYDLRQTTLDKAKERFNNYKKQPLDYIFPKIEVPQKFPF
                     NKDTIKPKAAIIREKGVNGDREMAYCLYLAGFDVIDIHMTDIINGKITLDDISFIVFA
                     GGFSNSDVLGSAKGWAANFHFNERARLELEKFYNRTDTLSLGVCNGCQLMVHLGLLSD
                     DKTLLPKMTWNDSKKFESNFITVDIHKSNSILLKPLIGSRLGVWIAHGEGKFILPGNE
                     INHNIVMKYSYHKYPANPNGSDYDTAAIASPDGRHLAMMPHIERSIFSWQWAYYPNDL
                     KKNVFTPWIMSFISAYDWITKKK"
     gene            complement(16203..16790)
                     /locus_tag="PKI65_02025"
     CDS             complement(16203..16790)
                     /locus_tag="PKI65_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOB77251.1"
                     /translation="MIFKIKTDKPIIFLTIDDGPCDISTPNTLNILDKYGIKATFFCI
                     GEKILKNKELSLKIKTEGHYIANHSFLHINGWKTKLNDYLLNVKLGENITNSKIFRPP
                     YGKLSPLQYLHLKKTNKIVLWSLMVYDFDNSRNIEKEKKLIENKLKPGAIIVFHDNEN
                     AVRKKDLLLPYLIELALEKNYTFHTLDQLNNFLTI"
     gene            complement(16815..17996)
                     /locus_tag="PKI65_02030"
     CDS             complement(16815..17996)
                     /locus_tag="PKI65_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOB77252.1"
                     /translation="MTLRTYMLSIFILAIFLFLYSFYFSPPQIKKKKWYYGWLIIIGI
                     VILYELIINFVVFSSGLSTILGAFYLYIFIFCTFYSIIHFIGWFFNGNIREKIQIYAV
                     KIAFIVVILSMVGNLIYSNKITIKNYILKYENLPNSFDGFTIAHISDLHLGSFVSDNK
                     LKKTTSKINNLNPDAIIFTGDLVNSSTKEIKPIHKECLKNMKAPIKLAILGNHDYHDF
                     HKFSHYWGKDKLQPNMKLADSISSILKECGFTVLRDSLYFVAKNHDTIVFIGLNNYGK
                     PPFTVYGNIYKVIDQIPNNLFSIVLIHDPFYWSEGLFDDIPLTLSGHTHGGQFGFFIN
                     SKLRLSFASINGKSGGLYKNDKNNKIIINTGLGHVAINFRFGFSSQICHIKLQKGRNI
                     D"
     gene            complement(18495..19085)
                     /locus_tag="PKI65_02035"
     CDS             complement(18495..19085)
                     /locus_tag="PKI65_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOB77253.1"
                     /translation="MKKDIILAGVGGQGILSIAATIGLAALKKNLNIKQAEVHGMSQR
                     GGEVMSHLRISDSPIFSDIIPKGQADMILSLEPLEALRYLPYLHESGWIITNSEPYKN
                     IPNYPDIDQIYNEIKKYKNHVLINADEIAKKLNAPLSSNMVLIGAATHYLGFEPKELE
                     DAIKEQFIRKGEDVININIQAFREGLAFAKEYILKK"
     gene            complement(19109..20719)
                     /locus_tag="PKI65_02040"
     CDS             complement(19109..20719)
                     /locus_tag="PKI65_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOB77254.1"
                     /translation="MVSNKLLLLGDEAIAQAAIDAGISGAYAYPGTPSSEILEFIINS
                     KEAKDKGIVSEWTTNEKTAMESALGVSYVGKRSIVCMKHVGLNVAADAFINSAITGAN
                     GGMVVVTADDPSMHSSQNEQDNRFYGDFAHVPILEPSNQQEAYDMCYYAFELSEKFHL
                     PVLLRITTRIAHSRAGVSRKSQLPQNQISLPKDLKQYILLPAIARKRWSHLLSLQPLL
                     LQESENSKFNQYIKGNDKSIGIIASGIAFNYVMENFNGNSPYPLIKISQYPLPQKMIK
                     KLYDEVDKLIVIEEGYPFIEKMITSFLCDDLKLEGKLTGLLPKEGELNPNIIAQALGL
                     PNIEGREVPSFVVGRPPSFCPGCSHIDSYNALNEALKDYSKGRVFSDIGCYTLGAMPP
                     FEAINSCVDMGASISMARTASDMGLRPSVAVIGDSTFAHSGMSGLLDAVVYNAPITIM
                     ILDNLTTGMTGGQPSHANQRLVSICSGIGVDPEHIKIIKPLKNKHQENVKIIREELEY
                     QGLSVIISSRECVQTLNRRMREKFKEKK"
     gene            21073..22101
                     /locus_tag="PKI65_02045"
     CDS             21073..22101
                     /locus_tag="PKI65_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOB77255.1"
                     /translation="MTKINNKKRFNILLTLFFIIIIISFLTPIIAPDKSYFANQQHPE
                     LAYLPMGTKVTFLIKKNESESFFLKKLLFGSNDYEEMISIQSYKLIENQLNIITYKEK
                     EIKSLEWNPEFMKIKEIKFWFGTDRLGRDVLSRIMWGARISLLVGIISTIISIILGVL
                     MGVIAGFYSGWVDKLVMFFVTVLWSIPSILIAMAISFALGKGFTPVFLAIGFTLWMDI
                     ARIVRGQVISLKQEDYINGLKTLGFSNSYILFHCIIPSLNGIITILAASNLSTALLLE
                     SGLSFLGIGIQPPMISWGAMIQEYFGHLLLPTYHLALIPGLCLTIIILLTMVLGNTLR
                     DILDIKSI"
     gene            complement(22166..23878)
                     /locus_tag="PKI65_02050"
     CDS             complement(22166..23878)
                     /locus_tag="PKI65_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOB77256.1"
                     /translation="MKKFILGLLISLCSITFLWSQKVEIDEAINVAKNFENTKKIFKE
                     SIILAHSTPYYYVFINEANNAFIIIGGYKQIYPILAYSTESGFDANYITPPVEWYLNG
                     IESQAKMAILDPDATERFKEAWHNTLKIGHPETKDIQAVGPLLSTKWDQGCYYNALCP
                     VDPNGPCGHVWAGCVATSMGQIMRYHKYPTSGIGTPSYASSYGTLTVKLDTVTYDYDD
                     MPNQLNAYVDSIPTAKLLYHCGITVEMGYGPDGSGAQSSKAALAFQNYFKYPDYVQYV
                     SRGSMDDDGWGSILKIEIDAGRPIYYSGGGLPGEAGHAFVCDGYDGPTHFHFNWGWGG
                     YLNGYFYLNNLKPGGNDFTNGQDIIIGVEPAEDDQVYCDELTILTDESGVIEDGSGEA
                     RYGNNSSCSWIIKPESQPASIVLKVEELALEEESDYVVIRAGESSTGPLIAYLTGHTL
                     PSPNIYYIMSPSVYIQFYSNETLRDYGFKISYESGDAINLIDNINLSIYPNPASDIIF
                     VETNEKIINVNIFNNLGQSIFEQNYESQSKIQINIDNINPGIYIIEVETISGFKTRKP
                     INIY"
     gene            24025..25191
                     /locus_tag="PKI65_02055"
     CDS             24025..25191
                     /locus_tag="PKI65_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOB77257.1"
                     /translation="MKINSILVHGGDIEDELGSVITPIYQTSTFKFKDAKQGADRFAG
                     KESGYIYTRLGNPTIRALEKKLALLEHGYDALACSSGMAAVNTTLLGLLKSGDHIIST
                     NAIYGCTFGVLTESYMKFGVEISFVDTSDLALIEKNIKPNTRLIYIETPANPTMKISD
                     IEAISKLAKQHNLYTVVDNTFASPILQNPLDLGADVVVHSLTKYINGHADIVGGAVIT
                     KTQELYNILYETFKNMGFNMDPHQAYLVTRGAKTLNLRVLKSQESAQIIAEYLEKHPK
                     VDSVAYPGLPSNPQYDLIKKQMKGPGSMISFELKGGYEAGFKLMNSVKLIALAVSLGG
                     IESLIQHPASMTHSHVSEEAKKAAFITDGLVRLSVGIEDIEDLLEDLDQALNQI"
     gene            25585..26805
                     /locus_tag="PKI65_02060"
     CDS             25585..26805
                     /locus_tag="PKI65_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOB77258.1"
                     /translation="MILNKVINDPIYEFIPIPDEIILSLIDHPYFQRLTRIKQLGFSY
                     LVFPGAHHTRFSHSLGAMYLMQRALTQLKYQGINIDDTLYLASLIAILLHDLGHGPFS
                     HTLEALLADNVTHETITNTAMLELEKTYGEIMQIARQIFNDVYPIKFYHQLVSSQLDM
                     DRLDYLTRDSFYTGVSEGIINTQRIIHMLTLTPNGDLGVLAKGVYSIEKFLIARRIMY
                     WQVYYHKTTMAADEMLYQVIRRAKFLTKNGTTVNATEPLEYFLKYNKTEFTNDDLKVF
                     MLLDDYDVMTALKMWTQHDDKILRLLSSKLLNRNLFRCLILNDMPSDKNINEIEKKIL
                     KKFAIDRDDLSFLMNIRPIENLSYSLQDNQINLVYKNSEIKDISQVSNQLNLDVLSMD
                     TTKYFLLYPKEIEK"
     gene            27025..27933
                     /locus_tag="PKI65_02065"
     CDS             27025..27933
                     /locus_tag="PKI65_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOB77259.1"
                     /translation="MSIKKLEEIVKTVQNQKSKRLVVAFANDIHTLEAVNTAYKLGFI
                     EPILVGDEKNIKELANQKNIDISKYKIIQESNDMNAARKAVELIRNGDADVLMKGTVT
                     SDKYAKAILDKERGIMPPKGVLSHVTVIEIPTYHKLLLVSDVAIIPTPTLDQKVAMAN
                     YLIRVAHKLGIEKPKIAVIAPSEQVLPAIPSCAEAAILSKMGDRNQIKGALIDGPMAL
                     DVAIDKESVEIKKIESSVAGDADCLLFPCIESANTFFKACTKIARGELAANVMGASAP
                     AILTSRGDSSLTKTYSIALACLMAEK"
     gene            27940..29022
                     /gene="buk"
                     /locus_tag="PKI65_02070"
     CDS             27940..29022
                     /gene="buk"
                     /locus_tag="PKI65_02070"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOB77260.1"
                     /translation="MDISLILVINPGSTSTKIAIFDSDGNKIYQNNIKHSAEELAPFS
                     KITEQFSFRKEIIIEELKKNNIDINKIKVIMGRGGLLKPIRSGLYEVNELMIEHLRNS
                     INGEHASNLGALIANDIAKSIPDARAFIADPVVVDELQDVARIAGHPLFKRISIFHAL
                     NQKAIARAHAKAQGKNYEDLNLIVVHLGGGISVGAHQKGKVIDVNDALNGEGPFSPER
                     SGTLSAGQVVDICFSGKYTKAEIKKMITGKGGLVAYLNTNDAQLVGKIILEGDKYAEL
                     IYKAMAYQVAKEIGACAIVLKGKIDAILLTGGIAFDNIFTDWIKDYVSFLAPIFIYPG
                     EDEMFALASYGIAYLKGEANVNVYGE"
     gene            29118..30095
                     /gene="rfaD"
                     /locus_tag="PKI65_02075"
     CDS             29118..30095
                     /gene="rfaD"
                     /locus_tag="PKI65_02075"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOB77261.1"
                     /translation="MSKYIIITGAAGFIGSVFAHYLNNNEIDKVIIVDDFSKKEKLLN
                     YDTLPYERIQRNLFIDWLEKYHNDVDAVIHLGARTDTTEFDYSIFEKLNLNYSKNIWN
                     ICSNHGIDLYYASSAATYGDGSLSYDDNLEIIDRLKPLNPYGLSKNEFDKWAIKQINQ
                     PKHWAGFKFFNVYGPNEYHKKRMASVVFHGYHQIKNEGKIKLFKSHRPDYKDGEQKRD
                     FVYVKDVIKVLFWFLEHRPDNSIYNLGTGKARSFIDLAKSIFAAMNIPTNIEFIDMPT
                     DIRDKYQYFTEANMAKLYNIGYNNSFYSLENGINDYVKNYLIPDKYYKK"
     gene            30092..30847
                     /locus_tag="PKI65_02080"
     CDS             30092..30847
                     /locus_tag="PKI65_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77262.1"
                     /translation="MKKILFLTLFVLISFFSFGQKLVDSTKTLSLIAHYSFDIPALDM
                     KARYGNNSGVGAELSYKTSKNVLFALDYNYIFGNNVKIEDSIFKGISTADGFILDQNG
                     MYAEVFCYERGFSIIAKAGYQFQLPTKYYSGIQVMGGGGFLQHKIRIENRENVAPQIL
                     GDYAKGYDYLRNGPAGYLYVGYVFHANKKIIKFTAGFDCLFAFTQSRRDYLFPIQGPD
                     NDTHLDIIYGFKVGWIINLYKSQKSNKKQYYFN"
     gene            30863..32677
                     /locus_tag="PKI65_02085"
     CDS             30863..32677
                     /locus_tag="PKI65_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77263.1"
                     /translation="MKNIPDIGPVKKKILGIKISESVIFFIVFVAVFFHIFFTHNRKN
                     LGQWEYPIWADAAAYFVYLPATFIYHYDGSKMPENISALTGDGFHIENNKIVTKVAMG
                     VAILQSPFYAISYLIANIFDINDNGFGKVYIYGLGVGAAFYFTLGLLFLYLFLKRRYS
                     KNICFITLLLIIFGTNLYYYTYEKFLYSHLYSFFIFSVSLYLTDKLIKTNFKKKIYIA
                     LFGIILSLAFLVRYTNPIFIVLMLLFLDIDSWQALKQRFKYIFNIKFISIAAISFIII
                     LLPQMFYWHYLTGNFLYYSYGNEGFNWLEPKFLEFFFSPDNGAFIYTPLYIIIFIYAI
                     IMSIKKEQNGLLILSIFLIASYIFASWWSWSYGCSLGKRPFVELTPIFSICIAGLVDK
                     IIEFRKPIISIILSLILLFFTLFSFSLNLHYAGCSFVSDTWDWNEYKLIVNRSRFPLI
                     RFHKYEYSTDFEYFNKELYYPFIFNTDLITKTDTAYSGNYVCRMSPDDLFSFTYRRDF
                     GTITVKDSCNVDFNIKYFFPNIDSNKLNAYLVIQVEEMDSAVYYASQKLESIQNKWLD
                     ANINVNLPNFPNYFVLVAYVLNDSSTILLDDFKIRIKF"
     gene            complement(33166..34719)
                     /locus_tag="PKI65_02090"
     CDS             complement(33166..34719)
                     /locus_tag="PKI65_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB77264.1"
                     /translation="METKNCISKRISFEIANSVNNILVYLISFVFSMFIFSCILQAQG
                     SRQDSINAFVRWRYIDATSTGTLCRTDNTGFYQLINWDINNRRAALYNNNDSIPLWEF
                     PALVNSIAMSRNGNLVAISSPDKIYVLDKITGDVVNEFILNVIPGAISGGPIEMVSDG
                     SFIIATASAGDSDSCWVFGFQSGSTSEIWKHKIYHSAYGMKISGNDSIVIVNSYNQVA
                     VYETFTGIERFSTTINGCQNSMGISGNGDIIATIDLHGWLKVYHWDGSNYTLLWQRME
                     PPTNYYNWMSAVDVSYDGSLIACGTLNFPTDTTEDGKVLLFKTTSGPTPQWTYTGTGN
                     IVTSVSFSKNGNILSACSWGDKSNLINDLLIFNTESAIPIFSLNSPGSFFSCSTSSNG
                     ATVIASGKSVHAGIFGHGGMVYNIAVDTNNINAVNNSIIEDFDSFSCFPNPFVSTTKI
                     KFELKENTNVIIDIYNMNGNKIINLINKEMNAGIHTIEWNAENYNAGIYFCRIITEKG
                     NGILKIIHL"
     gene            complement(35003..35314)
                     /locus_tag="PKI65_02095"
     CDS             complement(35003..35314)
                     /locus_tag="PKI65_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77265.1"
                     /translation="MRISGFTFCKNANKLYYPIKQSILSILPIVDEFIVNISDCDDDK
                     TVELIQSINSSKIKLIFSEWNSEKYPNGTENAHQTDIAKNACSGDWLFSWTQTEIGQG
                     L"
     gene            35598..36824
                     /gene="clpX"
                     /locus_tag="PKI65_02100"
     CDS             35598..36824
                     /gene="clpX"
                     /locus_tag="PKI65_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOB77266.1"
                     /translation="MATKNNDNIKCSFCGRNASEVDLMLRGFNANICNFCVEEAKKQI
                     NLQSTKSKKTPKLKLKKPTEIYEFLNQYVIGQDYAKKVLSVAVYNHYKRILNPIIKDV
                     ELEKSNILLIGETGTGKTLLAKTIAKLLDVPFAIADATALTEAGYVGEDVETILTRLL
                     QNANYDVKAAEMGIVYIDEIDKISRKNDNPSLTKDVGGEGVQQALLKILEGSVVFVPP
                     EGGRKHPEQEFIHLNTSNILFICGGAFDGLDKIIANRVKTSTIGFGVSQNNINNGEKN
                     ILHYVTPADLRKYGLIPEFIGRIPVITTLDSLNNEELKRILTEPKNALVKQYKALFEI
                     DNIELEFTEDALDAIVEKTIELKLGARGLRSVMEKTMMDLMYNTPHMQETKILITKKF
                     VEQHLQGFDNEQSKVA"
     gene            36821..37408
                     /locus_tag="PKI65_02105"
     CDS             36821..37408
                     /locus_tag="PKI65_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOB77267.1"
                     /translation="MKPRCFVLCILLFSFLKFNAQNNDDQILSRAIIYQGDTLPYVAL
                     DTVKYYYFDFKGSNKEYIAFTKLVRDVKKAYPYAKLVANKVNEINAYLNTIKDPKLLE
                     KEKNRLEKELRAQYEKQIRNLTRTQGKILIKLIYRETSKTSYNLIKDYRGSFLSSIYQ
                     GIAKIFGLNLKDTYDPEGKDRNIEIIVKLIENGQL"
     gene            37522..38946
                     /locus_tag="PKI65_02110"
     CDS             37522..38946
                     /locus_tag="PKI65_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOB77268.1"
                     /translation="MKNFFLFSILFYISINIFSQQEWSLEQCIQYGVQNSLNIKNQAL
                     NAEIAKANHLQSKLALLPDIHAAATHSYNYGKVVDPFTNDFAMDRIQTNNFYLASSIV
                     LFNGLKLLNNIKEQMSNTNTAELLLKKTENDVMLNIATLYLQVIFDSLNISTIHQQVI
                     LSELQVNRAELLYESGSISEDNLLNLQAQLASEQSNLITAEGRYELDLLALKQAMYYN
                     DTVPLKIKIPTDLQPIIDKHVNIPPLDTILLYAFNNLPDIKVAEWQLQSAKYSFKAAK
                     GNFSPTLSLSLSYGTGYSTASKQITEYEYSGYDTIGITTGTPPNFVLAPHLNYNYQTI
                     PFKNQLNDNINQSLGLSLQIPIFNRGIYHNQYQIGKINLQIAENNLEQTKLNLQNIIE
                     QAYYDMVAAEKKLDARNLYLKAQKIAYYNQQERFELGLISSIDFMNSKNQYTQAEIQQ
                     LQASVELLFRYIILDFYQGKSLSL"
     gene            38943..40238
                     /locus_tag="PKI65_02115"
     CDS             38943..40238
                     /locus_tag="PKI65_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOB77269.1"
                     /translation="MKNNKKTKRILIISGVSLLVLVIILRAIGVIGGSNGLEVAVEKT
                     IRRNITETITASGKIQPVKEVKISPDVSGEIIEILVKEGDKVKAGQVLAKINTDIYKA
                     TYEQASAQLQAQKANLANTEARLKQTEAQLLNAQLNFNRQKQLYEKGAISSSEYETAE
                     TNYKVAKAEYEAAKQSVKAAEFTVASVEASMDEALKNLNRTTIIAPLSGVVTKLSVEV
                     GERVAGASQFSPGTEVIRIADMNELEIVVTVNELEIVRIHQNDTANITLDAYDDYTFK
                     GIVTDMAISTINTLSLTSADEVSTYEVKIRLLPSSYQNLLTNTETPYPFRPGMSAYVE
                     IITKKAFNVIAVPIAAVTVKTDTATMKLNEVVFVVNKDNKVEKRNVKTGIQDNQYIEI
                     IDGIKENENVVIAPYSLITNILKGDESVTIVSKSKLYQK"
     gene            40235..40939
                     /gene="tsaB"
                     /locus_tag="PKI65_02120"
     CDS             40235..40939
                     /gene="tsaB"
                     /locus_tag="PKI65_02120"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOB77270.1"
                     /translation="MKYILIIETSDKANSIAICDSNGKLINYKDEISDNFTHIELLHV
                     SIDKLFNECGIAFSDLCAIAVSSGPGSYTGLRIGVSSAKGYAYALDIPLISLPTLELL
                     ANGMIDHIDEKNENIILIPTIDARRMEVYMQMFDYQIHAITEPSAEIINELYFSKFLK
                     DKIYYWGGSGAEKLSDFFPDNHKLVHQVQYPLARYGVSIAIDKYNTHQYEDIAYFEPF
                     YLKEFIPGKPRVKGLE"
     gene            complement(41047..42126)
                     /locus_tag="PKI65_02125"
     CDS             complement(41047..42126)
                     /locus_tag="PKI65_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOB77271.1"
                     /translation="MLLFRKLGYIILLIWGVLTFLFILFNLLPSDPVRLIMGQRSDVA
                     TEEAILKKLGLDKPLYIQYVKYLNDISPLGFYSNNRENTFYYDNVYTEKSLTIIKLNK
                     VNLGFKPIVFHESYISGQPVWQLIKEAFPLTLILAIVTMLVALVLSIILGSISALKAN
                     TWIDRLIIVFSSIGMSLPSFFAALLIAWIFGYLLHDITCLSMHGNLIEVNELTYSTYV
                     KWQNLILPAFTLSIRPLAVFVPLIRNSMLEVLNTDYIKTAHAKGLSSYNIMCHHVLPS
                     AINPLITSASGWFASLLAGSVFVEYVFNWKGMGSLIVESLQNYDLPVLKAGIFIVALL
                     FIIVTLIVDLLYLILDPRVRDKGMK"
     gene            42436..>43330
                     /locus_tag="PKI65_02130"
     CDS             42436..>43330
                     /locus_tag="PKI65_02130"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77272.1"
                     /translation="MDNFNLLDYQALPKEQKRRFLDNLYQFLLENNYTAVDYQKLKIQ
                     STAPICPRCESENVIKAGVRDGKQVYKCKDCGRQYRETARTFVYRMRKADKMLDYLKC
                     MLEGKSLRACASEVGISLRTSFNWRHKILAAIKSLQGGISFSGIVEADELLMKYSEKG
                     RKYRSRKEYEKAKKKKHPKVAVLVMTDREGNLLFKHVGRKKVTNSQIREELSHRVTDS
                     NLICVKQKKEFKKAVKGTKSKKVVVNKTQIRRGVYSVQVVDKHVNDFKVWLRRFRGVA
                     TKYLQSYLMWFVITHKYLTSEI"
BASE COUNT        15565 a         6020 c         6510 g        15235 t
ORIGIN      
        1 tttggtgttg aatgaatgga ttctaatata tgtttcaatc aacatatgtt atatttacta
       61 actctctata ccgataccat gcccacctgt ccgaggatgc gaggttgagc attcgcccag
      121 tattgcttac agtcttgcct gtgaggtatt tacccatcac tacataccac atagtgtagt
      181 tttgcagata tttggtagct acacccctaa atttgaaata tatccaatta gtgaattcac
      241 tagccttttt cttgacggta cttaggctat atatacctcg cttttgccta ttgctagtta
      301 ctatttcttt gtgcttgatt acattttttc taaccttttt gaatgctttt ttattactgc
      361 tacacatgac tccatccttt ggcatcttag ccttcagaaa ttgctctatc tgttctggac
      421 ttagcttttt gccatcaaat tttttgaata ataaattgcc agctctgtca gtagcagcca
      481 atacggctac tttctttggc ttttgcttcc tggctcgatt cagctcttct gcatttttgt
      541 atattctgcc tttttcggaa taatccatca atagttcatc catctctact actccgaaaa
      601 aattgatatt atcttcgaaa ctttgtagag ctgccaatat cttgtgtcgc caagcgaagc
      661 ttgttggtaa gctgatgccc acttctttag cacaagctct gagacttttg cctgctacca
      721 tacatctgat gtagtcgagc attttttctt tcttttgtaa cctgtaaaca aaagttccag
      781 tggtaaccct aaactgtctg ccacagtctt tgcagcgata gttttgcatc ccattttgta
      841 aaccattttt aatgatgtga ttgctacgac agtctggaca gagtggcggg gcactgagta
      901 ttttcagacc ttgataatct actacatcag cttctaattt tttcattaga gaataaagat
      961 tgtctaagaa ttctttattc tcttccttac tcatagctag gtatttgctg gtattctcta
     1021 ctattttttt catgaattaa aatttttgta aaaatataaa aattatttaa catataaact
     1081 tgaaaaactt aaacatttat taatttattt ccaaaacgtt ataaatgaaa gtaaaaatca
     1141 atatttaaga aagattatga tttatttaat gaacaatatt tttaaacata tatgactaga
     1201 tttttatttt tttgaaaaat ctgaaattat aaattcatta catataattt ctattatttt
     1261 gcaataatta aaaaaaaaag gaatggttaa acaaaaaaac tatacaaaaa tttatttgct
     1321 tttagcatct attactggtg gaggtttatt atcactcagt tggcctttga gaggttttcc
     1381 tttattatct ttaattagtt tagtgccttt tttatggatt ttagattata tagaaaatca
     1441 cagaacagac tttgtaggag gcgctgtttt tcgatatact taccttggct ttttattgtg
     1501 gaatgctgct actagttatt ggatatggta tgctacggcc gtaggttcta tatttgtaat
     1561 tttagttaat gcagcactta tgtcttttag tctgcaatta gcttttaatt taaaaaaatg
     1621 gcttggtagt aaaagtaaaa ttggtataat ttacataatt ttgtggcttt gcttcgaata
     1681 tttacatttt aattgggagc ttagctggcc gtggctcagt ttgggtaatg tttttgctac
     1741 tatgccggct attgtgcaat ggtatgagtt tactggcatt ttaggtggta cattatggat
     1801 atggcttgta aatatattta tttactatat aattttatca atttacaata agcaattaaa
     1861 agaaagagtt ttttacattt atttgattat tttatttttt ttattagcaa taccacttac
     1921 atattcttta tttagatata ataatattaa tttaaaaaat tcagatgaag ttaatgtttt
     1981 agttttgcaa cccaatttag atccttacaa agaagaattt actatttctc cgcaagaaaa
     2041 tataactagg ataaaaaatt taatagatga gataaatgct gagaaagtag attttttttt
     2101 agcaccagaa agcaatatac aagaaggtat atgggaaaat aattttgatg attcgtatag
     2161 tataaatgaa ataaaaaaaa ttttaaaaga aaaatatcct aatgctttat acataacagg
     2221 tgcatctact tataaaattt ttgaagatgg tgaatcattg actagtacag ctagatacaa
     2281 caagtatcat gatttttatt ttgatgctta caattcagtc ttatttattg atacatctgg
     2341 tgtagtcggt atttatcaca aaattaaatt agttgctgga gtagagcaaa tgccttataa
     2401 aaaaattata aaaccaatcg agaaattagc tataaatctt ggtggtacta ctggtagttt
     2461 gggtaaagat tcagtacctt atgtacatcc acattataag gctaatattg gggttgctat
     2521 ttgttatgaa tcagtttttg gcgaatttat ttctgatttt gttaaacgtg gtgctaatgt
     2581 tttatttgta ataacaaatg atggttggtg gggaaataca gctggtcatc gacaacattt
     2641 ctcttatgca tctcttagag caatagaaac tcgtagagaa atagctagat cggctaatac
     2701 tggtattagt tgcgtaataa ataaacgtgg agatgtttta aagtcaacaa attactggga
     2761 aaaaactgct tttttaacta ctatgtctct ttggaatgat gaaactattt atactaaaca
     2821 tggtgactac attggtagaa tagcagtatt ttctttatgt ttaataattt taattgatat
     2881 tataaagctt attattaaga agattcatta atttagcatt attgaattat aaaaattata
     2941 aatacttatc tttcaaaatt ttgttttatc aaaaattata tcagacaatg atttcaactt
     3001 gatattataa ttttatagta atttattaga ttattaaaca aaattttaat ctgttaatcc
     3061 tgatataggg atttgcaatt ttttactaat agctattaac atttcttttg tcattttagc
     3121 aagatcatag tctggttgcc aaccccattc ttctcttgca cagctatcat caatagattg
     3181 tggccaacta tcggctatgg cttgtctaaa atctggatta taatcaattt taaaatctgg
     3241 gatatatttt ttaatttcat tagccaactc ttctggagta aaactcatgg cagttacatt
     3301 gaagcctgaa tgatgcttta gattatttat atcagcttcg agcagcatta tagttgcttt
     3361 aatacaatca gccatataca tcattggtaa gcgagtatca gcttttaaaa aacaattgta
     3421 atacttattc tttacagctt cataaaagat ttctactgca taatcagtag taccaccacc
     3481 tggtaatgtc atactactga ttatgcctgg gtatcttatt cctcgtatat ctactccaaa
     3541 tttcataaaa taataattac ctagtaattc tcctgatact tttgtgatac catacatagt
     3601 ggtgggttgt agtattgtgt cttgaggtgt gtttatcctg ggagtagtag gaccaaatac
     3661 agcaattgaa ctaggtacaa aaacacgttc caatttcaat tcatttgcta tctctaaagt
     3721 gtttattagc ccatttatat tagtatccca tgcttttagt ggttcttgct cacctttagc
     3781 tgagagtatt gcagctaaat ttataatagt gtctatgtta taatctatac atgcttgttt
     3841 aaatttatca atatctctaa catcgaattg cacagaaaat tctttctttg ctaattcctt
     3901 tggtagagga ggataataat atgctaatac tatattttct tctccatatc tttttttcaa
     3961 tgcaggtgtt aattctgatc ctatctggcc tgttgcacca attactaata tctttttcat
     4021 agtttttttt tttacaaact tactaaaaaa tattaattat atttagttat aaacttaaaa
     4081 tttttataga aaatattttt tattaatttt gctcactaaa aggtaacttt atgaagcgtg
     4141 tagatattaa aaaatctaaa ggggtagctc tcatcacagg tgcttctagt ggcattggag
     4201 aagcatttgc aaaggagcta gcagctatga atcatgatat aacaatagta gctaggaggc
     4261 aagataaatt agaacaatta tcagctgaat taaaaactaa acataatgta aatataaata
     4321 ttatcccagc agatttggcc acggaaactg gaatagaaaa aatagaagaa tatattagtt
     4381 gcactaaaga tttagattta ctaataaatt gtgctggttt cggtttgaga ggtgattttc
     4441 ataatctcga taaagaaaat atttctcaaa taatagatgt acatctaaaa gcaccttcta
     4501 gattttgtaa agcagctatt cctgtgatgc gagatagagg ttatggctat atcataaatg
     4561 tatcatctct agctgctttt ttccctttca aaggtagtgc tatctacgta gctactaaga
     4621 tttatttagt ttatctctca aaagcaatac aaaaggaagt acgacggtat aatattaaaa
     4681 tacaagcttt atgtcctggt gttaccaata caggttttca ttatactgaa gcatttaaag
     4741 atttcgatcg ttccacagta cctaaaaatc tatggatgca accagatgta atagctaaga
     4801 aatctataaa tgctttgaaa aatcgaaaag tagtttacat accaggcttt aaaaacaaag
     4861 tcatgggagt actaactcag attggcaatt tgatttttcc ctaattatcc ataataatat
     4921 aaaggttatt agtgcattta aaactaaaac ttcccacccg accataaatt gtaagttggt
     4981 ttttaatatt ttcataatag caaaggagca taatggacta aacaatacta cctgccatat
     5041 actacctcca tctatattag ctttggttaa aatgccaaaa gtaaataagc ctaataatgg
     5101 cccataagta tatccagcta tagtgtataa agtatccaat attgcattat tatttaccat
     5161 ataaaataac attatcaata taaacatgcc cgctgatacg caccattgta taagcattct
     5221 atatttactt aatttttgat ttgatttgat tttttttggt aaatctaata gatctattgt
     5281 aatagaagta gtgagagctg tgagggctgc atcagtacca gaaaaagatg ctgctatcaa
     5341 gccaataaaa aatattatac ctaccatttc attcatatag taaaaagata ttgtaggata
     5401 caaataatct gtcgtttgca gtatagctat atctttaaaa tcagcataga gatacaatga
     5461 gccaccaagt atcaaaaata ttaaatctac tattgctagt ataatactaa atattagtat
     5521 gtttttttgt gaatttttta aagtagatat agataaattt ttttgcatca tctcttgatc
     5581 tagtccagtc atggaaatag taataaacat gccgcttagt aataatttag cccaaaaatt
     5641 ttttgatgat ggatctttgt ctattatata aaaatactca ctatgaaata cctgatttag
     5701 cagctcattc acgtcgatat acataaattg agcaataaaa tatattgtca atattaatga
     5761 tgctatcata aatgtagtct gcagtgtatc tgtccataca atagttttga ctccacctac
     5821 gagtgtgtat ccatgaatga tcagaaggaa aatgatagta ctaaaccaga atggtatacc
     5881 gaaattgctg agtacaaaat gctgaagtat tactattact ataaacattc gcaatgaagc
     5941 tcctatcgaa cgtgaaatta taaataacca cgatccagat ttttgagctt gtatgctaaa
     6001 tctatttttt aaatattcat atatagaggt gacattatta ttataatata taggcaaaag
     6061 cacaaaggct attacgatgt atcctattac gaatcctaat actacaacta aatatgtgaa
     6121 cccattatta cctactaaac ctggaactga tacaaatgta actccagtca atgatgtacc
     6181 tatcatgcca taagctacta aataccatgg agcctttcta tttgataaaa aatatgattc
     6241 attatttgct tttcgcgaag ttagataagc tgctacaaat agcataaaaa cataagataa
     6301 aaatataacc agtaaaagtg taggattcat ttttttgatg acaaaattaa ataaaggata
     6361 ataaatgtaa aaatttggta ttattattta aatagctagt taagatgaac caaaagatta
     6421 gatatttaga atttttttta aaaaaataaa tcgtaaattg tttattattt gcaatttaga
     6481 attattatat tgaaatcaaa acttatatga tttaaacaac ttataagctc gtcaagccat
     6541 ttctgattaa aatatttttt tggttctata acgtttattg tgggtaatca aattttagat
     6601 tgttacataa aaataactat ctttaattat ttaggttgtt tattttacca cagagaacac
     6661 caagttatta cacaaagttc acaaagtgcc atttatagtc tttttaattt catccttctc
     6721 tgtgttcttt gtgcattctt tgagaacttt gtggttaata tttgattatc ctataacaaa
     6781 aaaaccaata ttaagaaatt ttttataaaa ctactctttt ttttaatctt tacccataca
     6841 aaacgttata gaaccatttt ttttaattta ttttttttac atttgttaaa aaaaaataat
     6901 ataaaaatga ataccttatt attaagtgct ggattaggaa ctaggctgag acctttgact
     6961 aatgagatac cgaaggcact aattgctgta gcagatatac caataattaa acgtaattta
     7021 gataaattag cttcttacaa tgtcaaaaga gtagtaataa atgtacatta tcttgccgat
     7081 aagataatgt attatttatc taatttgcat tatgataata tggaaattat aatttctgat
     7141 gagaggtctc aactgttaga tactggtgga gctttattaa aagctaaacc attattttta
     7201 aaaaatgaac cgatattaat acacaatgta gatatattga gtggtacgga ctttgataaa
     7261 cttatgaagt acaaagaaaa aaataaaata gatgctttat tggtagtgca aaatcgaaat
     7321 aatcttagaa aattaatatt tgataaaaac gataatttgt caggatggat taattatgat
     7381 agtttagata aacaattaac taatgtaaat gaaaaattta caaaagaata tagtttttct
     7441 ggaatcagtt tgatttctta tgatctatta gatgatatac catttgatgg agtttttagc
     7501 attattacct tatttcttta ttgggcaaaa cgatataaag tatcttatta cgaagataat
     7561 gatatatatt ggtttgatat tggtacattt gatacattac aacaagctaa tgagtatttt
     7621 aaaaaaatag atcgttgagt ttatttcaga tatcaataga tgataagatt tttaaaaaat
     7681 tattttttaa ttcttctatg ttgccttcat tttttacaca gatatctgcc attttacatt
     7741 tgatatctgg tgataattga tgtttatcta tttgtaaaaa tcttttctta cttatttttt
     7801 tatttctata ttttttaatc cttttgtatc ttagaattaa tggtgcatat atacagatag
     7861 ttaaattaca aaatttatca agtgaatatt caaaaagcaa agctccatct atcactatat
     7921 atttatcatt taaattttta attgtattgt ctactaatgg aggtaaaaac tcaaggaata
     7981 attctgttaa ttgcttaaat ttattttcat ctgaataaat cttgtctgct atatcttggg
     8041 ttctcaatct atcattatat ataggatcga agcctaatat atctgttgct tttctacgaa
     8101 aactgtaatt aaaatataag tttttggcat ataaatccat atctataaca taaaaattgt
     8161 aatcagcaaa ccatctagca aaagtacttt taccgctgcc gatattacca gtaatgccaa
     8221 taatatattt gtccattatt atttgatgtc aaatatttaa cacagtctga attttttttt
     8281 attttgacaa aattaactaa aaaaaatcac gaaagatata ctaaatttta aaacacattt
     8341 aaggctaatt attacatgaa tagaatatat aatgatttat tttaattaac atatgaataa
     8401 gtgattacta atttcatacc attatcagat ccttttaata ttactctatt agggtttatg
     8461 atagcgccag cgactctaag gtataagcct agattttcat ttttgtttag taataaattt
     8521 tgagtataac gaggtaaacg aaaacgataa tatttttttt ggttataata atatccatca
     8581 aatagtgatt cgctaaagat ataatcttct aagtaagcgg ttttaccagt agaatctaca
     8641 attttagcta aagttaattg attaggtatg ggaaattctt tagcagtagg atcataagaa
     8701 gttggaatta tcagttccgc catatttatg tttacattct tacctttaaa ttcagacttc
     8761 aaatctggaa aacgtataaa aactttagtg ccagccatag gttgcaaaaa taaaatttgt
     8821 ttgcccaaac tggtgtcacc atttatttga cttaataaat tgtaagaagc aaaagtgtaa
     8881 tcatgttcga aataagaata ccattgcaat gtagcatcca tttgaaatac ttcttttact
     8941 ttagatagac tactagtatg gtaataaaga gtaatatttg acaaaatatt agttagatcc
     9001 caaacaatga gattgccttg attgttttct actacaggtt ctactactat tccataaaaa
     9061 tgtttttgaa aagtctgatt atttaccaat tttccctcga atgaagcatc aattattttt
     9121 ttagcaaaat tcaatgaaag tggtattttg agtgtaggag ctgcctttac actgtctatt
     9181 aatatgctat ctatttgtga gggattaaag gtgattttgc ctataaggtt cccttttttg
     9241 ataggtctat tagaataata agtactgtcg taatagagag gttctcctaa ttcataaaca
     9301 ttgaaagtca ttacatcatt tttacgagat tttgcataaa tattatttaa ttttaaatat
     9361 agattaatag aatcaggtac tgcatcctcg ccaaatatta tattactttt agtaatcttt
     9421 aattgactac aaaaactact agtagttaaa ccaaatatcg gatcacgaag gtacccagct
     9481 atattatatc tgcccctaga gcttagcaaa ctatcatctt tcaatgtata tgctgtcaat
     9541 aaaaaggtat ctacatttac attaatgcga tcactcggcg gatgtatttc caagcctatc
     9601 tgttcatctt gatcacagct aaataaaatt aatacaaaaa taaataaaca gaaaataaac
     9661 ttcttcactt tttttataaa ttacgttttt gatttatcaa tattattttt tttaaaaaaa
     9721 aaattaattt tacaaattta tgaattttat tttttataaa tctaattaat aagatataaa
     9781 ttgtaattag ataatttttt atttacacaa aaactatata agttataaaa ccgcaatata
     9841 gtagatttaa tgaattgtgt tgctgttttt tgcagtatta atctgttata tattagaata
     9901 tttgtatttt tatcaaaaat aataaatgtt taaaaatact aacaaagaaa taggtgattt
     9961 aggcgaaaaa atagcaggta aatatttgat aagtaaaggt tatgaaatag taggacaaaa
    10021 tattagatgg aaaaaatatg aaatcgattt aataatcaaa aaagatgatt ggctgatatt
    10081 cgtagaggtt aaaactagga agatgggcgc atatattcct gctattgaaa gtataaataa
    10141 aaacaagctg caagccatga gcaattttgc tgataactat ataaaagaaa ttgactggaa
    10201 tggcaatatt agattcgatg cggttatcat tagctattca tatttgacgc cgcctataat
    10261 aaagcatata gaaaatttat ctattgaatg aaataattaa atcctaatta caatatgcaa
    10321 aagagagtgg gggagtaggt aaaaaatcgt aggttgttag ttgaaatttt ttgattaatt
    10381 tatgattttt catttatata tatgtatctt ttttaaataa atattttata tttgcttatt
    10441 aaatggacta aatgcttagt agaaaattgt ttatctaaaa aaaaataacg aattttgcaa
    10501 attatattta atttatgcaa tacaagtctc gtcccatacc acgtagtatt attttgttag
    10561 ctgacatact aatagtagct tttacttata tatttagtta ttttctaaga tttaatttca
    10621 aaattccaat tgcagagcaa aacacattat atataggatt atttgtaata gtagttgtca
    10681 aattgattta tttcctatca cttggtacct ataaacacct tatacgtttt gtgagtgcta
    10741 atgatgctat tcgtatgttt tttagtttag tttttagcgt tatcactatt tcgattataa
    10801 atctattatt atataatata gctcacatac catatatagt tcctttttct atattaataa
    10861 ttgattttgt atttagcgtt ttctttttat taggttatcg tttagctatt aaagttttgt
    10921 tttttgagtt ttttaatgga caacgtgaaa aatctaatgt aattatttat ggtgcaggca
    10981 gaaatggtag tttagtaaag caagttttag aaagagatgc agggacgaaa tataaaattt
    11041 tagcattcat agatgaggat cattataagc aaggaaagca attagatggt attcccattt
    11101 ataacccaga aagattagaa gagtttttaa agagcaatac agtagcacat cttatcattt
    11161 ctatacctaa tatatctcct aacaaaaaag cttacttgac agagctatgt ctaaagtata
    11221 aaacaaaagt tttggtagta ccacctgtga gtaaatggat taatggtgaa ttatctttta
    11281 aacaaataaa aaaaatacgt ttagaagatc tattgcaaag agaagagatc aatattgacg
    11341 agtcagacgt atataaaact ctatataata gaatcatact aatcaccgga gcagctggta
    11401 gtataggcag tgaattagtg agacaatgta tcaggtttaa tccattcaaa attatatgct
    11461 ttgatattgc agaaacccca atgcataatt taaaattaaa tttagaaaat gaatttactt
    11521 atgaacaatt agaaaaagtc caatttattt taggtgatat taccaataaa tatgacgttg
    11581 agcaaatatt caatgaatat aaacctcaga ttatttttca tgcagccgca tacaaacatg
    11641 tacctatttt agaatatttt cctaaaaaag caattgaagt taatgtttat ggcacttatc
    11701 tagtagcaaa tgcagcaata aaatataatg tagaaaaatt tatcttcatc tctactgata
    11761 aagctgtgaa ccctagtaat gtcatggggg ctagtaaacg attggctgag ctactagtaa
    11821 aatatttaaa tgatacttcc catactcatt ttattactac gagatttggt aatgtgattg
    11881 gtagtaatgg gtccgtattt gaaatattta aacaacaaat tgagagtggt ggtcccatta
    11941 cagttactca tcccgatgtt acacgttttt ttatgactat tccagaagcc tgtagactag
    12001 tgctagaagc cgctagcata ggaaggggag gggaaatttt tctttttaat atgggtgaaa
    12061 gaattaaaat attagatctt gctaaaactc ttattcaatt gagtggactt acattaggta
    12121 aagatataca aattaaattt tctggtttaa gaccaggcga aaaaatgaca gaagaattat
    12181 taactgatta ctcaagagat atagaaacta aaaataaaaa tgttttaatc tcttctgaac
    12241 aaataataga tttagatgat aaagtaaaaa atatactttt tttgataaac aattatcaga
    12301 aaatatctaa taatgagatt gtagaaaata ttcaaaatat tataccagaa tttaatagta
    12361 tgaatagctt atattctcgt ttataaaata aattcattaa attttttata taaatatgaa
    12421 aatatatttt ttttattcac cagacaatca aggatatata gctgtagaat ctacaagatt
    12481 gtcactaaaa aatattgata aacttttgtg gttatttggt gatgatgctt ttttattaaa
    12541 tgtagatact ttagttggcg aatttatttg tactttcccc gaaatgataa ctccatggtg
    12601 tactaatgct gtagagatag tgcacaatat aggtattagt tctattaaaa ggatggaaaa
    12661 gctcattcca tatgataaat ataaatctac aatttatgat cctatgttgc atactatcat
    12721 ttccgatcct tcacaaaatt tatttaaaat taaaagaaaa aaagaaccaa tcaaatatat
    12781 tgatgatatc gaaaaataca attatgaagc aggattagct ttatctaaat atgagattga
    12841 ctatctaaaa tacataagcg aaaccattgg taggccgttg acagattgcg aagtatatgg
    12901 atttgctcaa gttaactccg aacattgtcg acataaaatt tttaatggag tttttgttat
    12961 agataatata gagcaaaagg aaacactttt cgatcttata aaagctacta ctagagctaa
    13021 tcatggtaaa gtaatatccg catatagtga taatgtagct tttatagaag cagaaaaaag
    13081 caaaattttc actcctgtta gtggcgacat gccttcatca tatatagaat atgaagaaga
    13141 actagtttat tcattaaaag ctgaaacaca taattttcct accactgtag aaccttttta
    13201 tggtgctgct accggtagtg gtggtgagat aagggatagg atggctggag gtattggtag
    13261 tatacctttg ataggtacag ctgtttatat ggtagctgac actagaaatt ttattgaaga
    13321 aaaaaatctt ttcggtcaat cgaaaggtag atttctctat cataatccag tggatacatt
    13381 gataaaagca tctaatggag catcagattt tggtaataaa tttggtcaac cactaatttg
    13441 tggaagttta tatacttatg agcaaatgat agatgatcaa ttaatagcat atgataaagt
    13501 gataatgcta gctggtggta tcggttatgc tctaaaaaaa tatgcttata aaaaaactgt
    13561 tcgtcctggt caaactgtag tgatgatggg gggagataat tatagaatag gtatgggagg
    13621 tggtgcagta tcatcagtag ctactggaaa atataataat agtgttgaaa gaaatgctat
    13681 acaacgtgct aatccagaga tgcaaaaacg ggtcttcaat gttattcgcg ctctcgcaga
    13741 gaatcattta aatcctatca tttctattca tgatcatggt gctggaggcc atctaaactg
    13801 cttcgctgag ttactagatc ctataggtgg cgaaattaat atcgatgctc tacctctagg
    13861 tgatccatct ctatcagata aagaaataat atgtaatgaa tctcaagaga gaatgggatt
    13921 ggtagtggat aatagtaatt atgaaaaaat cgaaaaaata gctctgcgtg aatgtgcccc
    13981 tatttataaa gtagggaaaa tatatgctag taattctatc tgttttgtcg gcaatgacaa
    14041 tagaaaacct tttgatctaa aatttgaata tttatttggc aaaactccta aaaaaattat
    14101 tagagattat aataaaaaat atgaatttca taatcccaaa tataatttga atgagttcga
    14161 tacgtattta gaaaaagttt taactaatga tgctgtagca tgcaaagatt ggttgacaaa
    14221 taaagttgat agaagtgtaa caggtaaagt agccctccag caaacttgtg gtaaaataca
    14281 attaccttta aataatctcg ctataacagc tctagattat acttctaata ctggtattgc
    14341 tatcagcata ggtcacgctc ccaatgtagg aataatagat cctgctaaag gtagtaggct
    14401 atcagtagct gaagccttga ctaatattgt atgggtacca ttaaaggatg gattagaagg
    14461 aatatctctg agtgccaatt ggatgtggcc ttcaggatta ccaggagagg atgctcgctt
    14521 atatgaagct gtaaagtctc ttagtgactt ttgtataaaa cttaaaatca atattcccac
    14581 aggcaaggat tctctatctt tatcacaaaa ttatcctgat ggcacagttg taaaaggtcc
    14641 aggcacagtt gtgataactg ctagtgccct tactaatgat tttacaaaag tagtaactcc
    14701 agatattaaa aatgtagaaa attcatctct tatttttatt aatttctcta aaatgcctcc
    14761 atccttgggt ggtagtattt tttatcaaga attagaacag attggtaata atactcctga
    14821 tatcgtagat gtcgaatatt ttaaacgtgc atttatagtt ttacaaaatt tgataaatca
    14881 aaatctagtt ttagctggtc atgatgtcag tgcaggtgga ttgattacta ctcttctaga
    14941 aatgtctttt gctaatccag atattggatt agatattaat ctggcatcta ttccttatga
    15001 tgatatcata gctattttgt ttgccgaaaa tccaggtgtt gttattcaag ttgctaatgt
    15061 taaggaagtt ataaatcagt tatatcaagc tgaaatgcaa tactacccca taggtacagt
    15121 tatagacaaa agagaactta aaattataaa atatcaaact tatgaacatt ttgatataga
    15181 taaatggcga gatatatggt ataaaaaatc atatttatat gatttgcgac aaactacatt
    15241 agataaggca aaagaaagat ttaataatta taaaaaacag cctcttgact atatttttcc
    15301 taaaatagaa gtcccacaaa aatttccgtt taataaggat acaattaagc ctaaagcagc
    15361 tattataaga gaaaaaggtg taaatggaga tagagaaatg gcttattgcc tatatttggc
    15421 aggttttgat gtgatagata tacatatgac agacattata aatggcaaaa taacactcga
    15481 tgatatttct tttattgtat ttgcaggagg ttttagtaat tcagatgtgc taggttcagc
    15541 taaaggttgg gctgctaatt ttcattttaa tgaaagagct agattggaat tagaaaaatt
    15601 ttataataga accgatacat tgagtttggg agtttgtaat ggttgtcaat taatggtaca
    15661 cttaggatta ttatctgatg ataaaacatt gctcccaaag atgacatgga acgatagcaa
    15721 aaaatttgaa agcaatttca taactgtaga tatacataaa tctaattcta ttttattgaa
    15781 acctcttatt ggttctcgat taggagtatg gatagctcat ggtgaaggta aatttatcct
    15841 acctggtaat gaaatcaatc ataatatagt tatgaaatat tcataccata aatatccagc
    15901 taatcctaat ggctctgatt atgatactgc agctattgct tcaccagatg gcagacatct
    15961 agctatgatg ccacacatag aacgttcgat atttagttgg caatgggcat attatcccaa
    16021 tgacttgaaa aaaaatgttt ttactccatg gataatgtct tttataagtg cttatgattg
    16081 gataacaaaa aaaaagtaga ataataaaag cattaaaaat tttacatact aattattagt
    16141 agttaaaaaa aatcattctg ttttcaattt cccttagtct agtgttgttt ttttgtattt
    16201 attcaaatag ttaaaaaatt attcaattgg tccaaagtat gaaaagtata atttttttca
    16261 agagctaatt ctattagata tggtaataat aaatcttttt ttctaactgc attttcatta
    16321 tcatgaaata caataatagc acctggtttt aatttatttt caattaattt tttttctttt
    16381 tctatatttc tactattatc aaaatcataa accattaagc tccataaaac tattttatta
    16441 gtttttttca aatgtaaata ttgtaatggt gagagtttac cataaggtgg tctaaaaatt
    16501 ttactattag taatattttc tccgagtttt acatttaata aataatcatt taatttagtt
    16561 ttccatccat ttatatgtaa aaatgaatga tttgctatgt aatgcccttc agtttttatt
    16621 tttaaagaca attctttatt ttttaatatt ttttctccaa tacaaaaaaa tgttgctttt
    16681 attccatatt tatctaaaat atttaaagta ttaggagtag atatatcaca aggtccatca
    16741 tcaattgtta aaaaaataat tggtttatct gtttttattt taaaaatcat ctttacatat
    16801 ttaataattt attattaatc gatatttcta cctttttgca atttaatatg acatatttga
    16861 gatgaaaaac caaaacgaaa attaatagct acatgcccca gacctgtatt aataattatt
    16921 ttattatttt tatcattttt atacaatccg cccgacttgc catttatgct cgcaaaagat
    16981 aacctcaatt tactatttat aaaaaaacca aattgccccc catgggtatg tcccgaaagt
    17041 gttaatggaa tatcgtcaaa taaaccttca gaccaataaa atggatcatg aatcaaaact
    17101 attgaaaata aattattagg tatttgatca ataactttgt aaatattacc atatacagta
    17161 aatggaggtt tgccataatt atttaatcca ataaaaacta ttgtatcatg gttttttgct
    17221 acaaaataca agctatctct tagtactgta aatccacatt cttttagaat cgaagaaata
    17281 gaatcagcta atttcatatt tggttgtaat ttatctttac cccagtaatg actaaattta
    17341 tgaaaatcgt gataatcatg attgccaagg attgctaatt taattggagc tttcatattt
    17401 tttaagcatt ctttatgtat tggtttgatt tcttttgtac tcgaatttac taaatctcca
    17461 gtaaaaatta ttgcatcagg gtttaaatta ttaatttttg atgttgtttt ttttaatttg
    17521 ttatcagaaa caaaagaacc taaatgtaaa tcagaaatat gagcgatggt gaagccatca
    17581 aaagaatttg gtaaattttc atatttaaga atataatttt ttattgttat tttattagaa
    17641 taaattaagt taccaaccat actcaaaata actactataa aggcaatttt aactgcatat
    17701 atttgtattt tttctctaat attaccatta aaaaaccatc caataaaatg aataatacta
    17761 taaaacgtac aaaagataaa tatatataaa taaaaagcac ccaaaatagt agaaagacct
    17821 gaactaaaaa ctacaaaatt aattatcaat tcatacaata tcacaatacc tatgattatg
    17881 agccagccat aataccactt ttttttctta atttgaggtg gagaaaaata aaatgaatat
    17941 aaaaacaaaa atattgcaag aataaaaatt gataacatat aagtacgtaa agtcataaat
    18001 gagaagtttt tacaaaaata tgattttttc tgattttgat aaaaatagaa aaatattatg
    18061 aatgttttta attgttatac tttacgtatt ttataacaaa aacatcagat aagcctatta
    18121 atgatttcaa gatttggtta cgatgattta gaggtgtaac tatcagatat ttatgtattc
    18181 ttagatagat atgtactaaa tgagcattat gagcttgtaa atatgaaata taataaacta
    18241 cttaaagtat aacaatcagc aatttctctt gattaaaata atttcaataa aaaactatta
    18301 aaacaaatac gattattata atttttttta aagtagctta aaagataaat tataatattt
    18361 tctgttatta ttttattact taccaaatca ataaatttac aaagtttatt gatttgaatg
    18421 aatttataaa tagattctat gattttttta aatcaaaagt aaaaaaatga atttttagaa
    18481 cccatctgta attattattt ctttaaaatg tattctttag caaaagctaa tccttctcta
    18541 aaagcctgaa tatttatatt tattacatct tctccttttc taataaattg ttctttgata
    18601 gcatcttcta attcttttgg ttcaaatccg agatagtgag tagctgcacc tattaatacc
    18661 atgttagaag agagaggggc atttagtttt ttagcaatct catcagcatt tatcaggaca
    18721 tgatttttat atttttttat ttcattatat atttgatcta tatcaggata attagggata
    18781 tttttgtaag gttctgaatt tgttattatc catccgctct catgtaaata tggtaagtaa
    18841 cgtaaagctt cgagtggttc aagagataaa atcatatcag cttgtccttt aggtataata
    18901 tcagaaaaaa ttggagaatc agaaattctc agatgagaca ttacctcacc acctctttga
    18961 gacataccat gcacttctgc ttgtttaata ttcaaattct tttttaatgc agctaaacca
    19021 atagtggcag cgatacttaa tataccctgt ccacctacac ctgctaagat tatatctttt
    19081 ttcataatta aactatttta aaagtaaact attttttttc tttaaatttt tctctcattc
    19141 tgcgattgag agtttggacg cattctctac tagaaattat cacagataaa ccttgatatt
    19201 ctaattcttc tcttatgatt tttacatttt cttgatgttt atttttcaaa ggtttaataa
    19261 tttttatatg ttctgggtca acaccaattc cagaacaaat agaaactaat ctttgattgg
    19321 cgtgagaagg ttgccctcca gtcattcctg tagttaaatt gtctaaaatc attatggtga
    19381 taggtgcatt ataaactact gcgtcaagca aaccactcat accactatgt gcaaaagtgc
    19441 tatcacctat tacagctacc gatggtctca gtcccatatc agaagcagtt ctagccatag
    19501 aaatagaagc acccatatct acacaagaat tgatagcttc gaacggtggc attgctccta
    19561 aagtatagca acctatatca ctaaaaactc taccttttga ataatctttt aaagcttcat
    19621 taagagcatt ataagaatct atatgagaac atccaggaca aaatgaaggt ggtctaccta
    19681 ctacaaaact aggtacttcc ctgccttcta tattgggtaa acctaatgct tgagctatta
    19741 tatttggatt aagctcccct tcttttggta gtaaacctgt taatttaccc tctaacttga
    19801 gatcatcaca taaaaaactt gtaatcattt tttcaataaa tggatagcct tcttctataa
    19861 ctattaattt gtcaacctca tcatacaatt ttttaatcat tttttgaggt aaaggatact
    19921 gagatatttt tattaatgga taaggactat taccattaaa attttccatt acataattaa
    19981 aagcaattcc agatgcaatg atgccaatag atttatcatt gccttttata tactgattaa
    20041 attttgaatt ttcgctctct tgtaataata aaggttgtaa tgataaaaga tgcgaccatc
    20101 ttttacgagc aatagctggt aaaaggatat attgttttaa atctttagga agggaaattt
    20161 gattttgtgg taattgagat ttacgagaaa ctccagctct cgaatgagct attctagtag
    20221 ttatacgtaa taaaacgggt aaatgaaatt tttcactaag ttcaaatgca taatagcaca
    20281 tatcataagc ttcttgttga ttagaaggtt ctaaaattgg cacatgagca aaatcaccat
    20341 aaaaacgatt atcttgttca ttttgcgaag aatgcataga tggatcatca gctgtaacta
    20401 caaccatacc gccattagca cccgtaatag cagaattgat aaaagcatct gcagctacat
    20461 ttagtccgac atgtttcatg caaactattg atcttttacc tacataactg actcctaaag
    20521 ctgactccat cgcagttttt tcattagttg tccattcaga tactatgcct ttgtcttttg
    20581 cttctttact attaattata aattctaaaa tctcagatga aggtgtgcct ggatatgcat
    20641 aggctcccga gatgcctgca tctattgcag cctgagcaat agcttcatct cctagtaaca
    20701 ataatttatt agataccata ttttttgcac aaagataata tttaaaattt attataacaa
    20761 atcaatttat aagttttata caatcgcata gatttttctt attaataata aaaaaaagag
    20821 cttattcaga taaagtgtaa aaaagtaaac cgttttatta aaattaaagt aataaattca
    20881 aatgtttaca ttatcaagca ttataacttg acaaataata atatacttga tttatcacat
    20941 ttcaatgtgt ctatatttat ctagaatatt tttttattaa tgttaataaa aataagttta
    21001 ttgataatta aataagtaat tagcaattga taaaaatttt ctacttttgc tttatgactt
    21061 ttctcacaag taatgacaaa aataaataat aaaaaaagat ttaatatttt attaactctt
    21121 ttttttataa ttatcattat atctttctta actccgataa ttgctcctga taaatcatat
    21181 tttgctaatc agcagcaccc cgaattagca tatttaccaa tgggcaccaa ggttactttt
    21241 ttaattaaaa aaaatgaaag cgaaagtttt tttttaaaaa aattattgtt tggtagcaat
    21301 gattatgaag aaatgataag tatacaatcc tataaattaa ttgaaaatca attaaatata
    21361 ataacataca aggaaaaaga aataaaatca cttgaatgga accctgaatt tatgaaaata
    21421 aaagaaataa aattttggtt cggtacagat agattaggca gagatgttct cagtagaata
    21481 atgtggggtg cccgcattag tttattagtt ggaataatat ctaccataat ttctataatt
    21541 ttaggtgttt tgatgggagt aatagcgggc ttttatagtg gatgggtgga taaacttgtg
    21601 atgttttttg taactgtttt atggtctatt ccaagtatct taattgctat ggctatatca
    21661 tttgctcttg gtaaaggctt tactcctgtg tttttagcca taggtttcac tctatggatg
    21721 gatatagctc gtattgttag ggggcaggta atatcattaa aacaagaaga ttatattaat
    21781 ggtttaaaaa ctttagggtt ttcaaatagt tatattcttt ttcattgtat tattccttca
    21841 ttgaatggca tcattactat attagcagca tctaacttgt ccacagcatt attattagaa
    21901 tcgggattaa gctttttagg tattggtata caaccaccaa tgatttcttg gggagcaatg
    21961 attcaggaat atttcggaca tttattattg cctacttatc atttagcatt gataccaggt
    22021 ttgtgcctta ctataataat tttattaaca atggtgttgg gtaatactct tagagatata
    22081 ttagatatta aatctattta aaagaaaagt gagcttttta ataaaagctc acttttcttt
    22141 tttttttgaa ttttttttga attctttaat atatatttat aggttttctt gttttaaatc
    22201 cgcttattgt ttctacttcg ataatgtaaa tcccaggatt aatgttatct atatttatct
    22261 gtatttttga ctgagattca taattttgtt caaatataga ttgtcctaaa ttattaaaga
    22321 tattaacatt tataattttt tcattagttt ctacaaaaat tatatcagag gcgggattag
    22381 ggtatattga taaattaata ttatcaatta aattgatagc atctccagat tcatagctta
    22441 ttttgaaacc ataatcgcga agtgtttcat tagaataaaa ttgaatataa acagatggag
    22501 acatgatgta gtaaatatta ggtgatggta atgtatgacc agtaaggtat gctattagtg
    22561 ggccagtaga agattcacct gcacgtataa ctacataatc tgattcttct tctagagcca
    22621 gttcttccac tttcaatact atggatgcag gttgtgattc tggtttaata atccatgaac
    22681 atgatgaatt attaccatat cgagcctcac cactcccatc ttctattacg ccactttcgt
    22741 ccgtaagaat cgtcaattca tcgcaatata cttgatcatc ctctgcgggt tctacaccaa
    22801 taataatatc ttgtccattg gtaaaatcat taccacctgg ttttaaatta tttaaataaa
    22861 aataaccatt taaataacca ccccatcccc aattaaaatg gaaatgagta ggtccatcat
    22921 atccatcaca tacaaaagca tgtcccgctt caccaggtaa accgccacca gaataataaa
    22981 taggcctacc tgcatctatt tctattttta aaattgatcc ccatccatca tcatccatac
    23041 tgcctctaga tacatattgt acataatcag gatatttaaa gtaattttga aaagctagag
    23101 ctgctttaga agattgagca ccacttccat caggtccata gcccatctct acagtaattc
    23161 cacaatgata aagtagtttt gctgtaggta tactatctac gtaagcattt agttgatttg
    23221 gcatatcatc ataatcataa gtaactgtat caagttttac tgttaaggtt ccataacttg
    23281 atgcatacga tggtgtacct atcccagagg taggatattt atgatatctc attatttgtc
    23341 ccatagaagt agccacacaa ccagcccata catgaccaca aggaccatta ggatctacgg
    23401 ggcataaggc attataatag catccttgat cccatttagt tgataatagt ggacctacag
    23461 cttgtatatc cttagtttct ggatgcccga tttttaatgt attatgccaa gcttctttaa
    23521 atctttcagt tgcgtcagga tctaagatag ccatttttgc ttgactttct attccattta
    23581 aataccattc tactggaggt gtgatataat tcgcatcaaa tccactctca gtagagtatg
    23641 ccaaaatagg atatatttgt ttataacctc caataattat aaaagcatta tttgcctcat
    23701 taataaatac ataataataa ggtgtactgt gtgctaaaat aattgattct ttaaaaattt
    23761 ttttggtgtt ttcaaagttt ttagcaacat ttatagcctc gtctatttct actttttgtg
    23821 accataaaaa agttatgcta cataaagata tcaataatcc taaaataaac tttttcatat
    23881 tttttatatt tttaaatttt tgctatgcaa aagtaaaaaa tattttaaaa caagcaaaat
    23941 tttaataata tataattatt tttgagattt aatttgttat tacaaaaatt attacgaatt
    24001 ttgtcaagtt aaaaactaag tgttatgaaa ataaattcaa ttttagtaca tgggggagac
    24061 atcgaagacg aattaggtag tgtaattacc ccgatttatc agacatctac gtttaagttt
    24121 aaagatgcta aacaaggtgc tgatcgcttt gctggaaaag aatctggtta tatttatact
    24181 agattaggca atcctactat cagagcttta gaaaaaaaat tggctttgct agaacatgga
    24241 tatgatgcac tagcttgctc atctggtatg gcagctgtta atactacatt attaggttta
    24301 ttaaaaagtg gagaccatat cataagtact aatgctattt atggttgtac ttttggtgtt
    24361 cttactgaaa gttacatgaa atttggtgtc gaaatttctt ttgttgatac tagcgattta
    24421 gcattaatag agaagaatat taaacccaat actcgtttaa tatatattga gacgccagct
    24481 aacccaacta tgaaaatatc tgatatagaa gcaataagta aacttgctaa acaacataat
    24541 ctttatactg tggtagataa tacttttgct tctcccattt tacaaaatcc tttagatctt
    24601 ggagctgatg tagtagtaca ttctctcact aaatatataa atggtcatgc cgatattgta
    24661 ggtggcgcag ttatcactaa gacccaagaa ctttataata ttttatatga aacattcaaa
    24721 aatatggggt tcaatatgga tccacatcaa gcttatttag taactagggg tgctaaaact
    24781 ctaaatttga gagtactaaa atcgcaagaa agtgctcaaa taatagctga atatctcgaa
    24841 aaacacccta aagtggactc cgtagcttac cctggtttgc catcaaatcc tcaatatgat
    24901 ttaattaaaa aacaaatgaa aggtccaggc agtatgatca gttttgaact aaaaggtggc
    24961 tatgaagctg gtttcaaatt gatgaattct gtcaaattaa ttgcattagc tgtatcactc
    25021 ggtggtattg agagcctgat acagcaccca gcttctatga ctcattcaca tgtatcagaa
    25081 gaagctaaaa aagccgcttt cattacagat ggacttgtac gtctttctgt aggcatagaa
    25141 gatattgagg atttattaga agatctcgat caagcactta atcaaatttg atatattttg
    25201 cttttttgat gagaattatg agttaatacc tgaatcagta atataaatgt attgaaagca
    25261 tctttgcttc tataggttta tgtgacaata aatgcgttgc tgctaaattg atgctattat
    25321 tttccttaaa tttaaatatt ctatattttt gcagctttta tgtactattt agcaaactca
    25381 cttctggttt ttcatttctt tgtcttctga gtactttgat atcagatttt ttattatttg
    25441 taattaaata ctgtacctag aataatgcga cttttattat tttaagtatt tataatattt
    25501 tttgtttatt aggtcaactt atctcatgct tttgcaaata gggataatta ttatattttt
    25561 gtaaaaaaag cacaatatta atatatgata ctaaataaag taattaacga tccaatatat
    25621 gaattcatac ctattccaga tgaaataata ttgtctctaa tcgatcatcc atattttcaa
    25681 agattgacga ggataaaaca attaggattt tcttatttgg tatttccagg agctcatcat
    25741 actagattta gtcatagttt gggagctatg tatctgatgc agagagcttt gactcaatta
    25801 aaatatcaag gcattaatat agatgataca ttgtatttag ctagtttgat agctattctt
    25861 ttacacgatt taggacatgg acctttttct catacattag aagcattatt ggcagataat
    25921 gtgacgcacg aaactattac aaatacagca atgctagaat tggagaaaac ttatggagaa
    25981 attatgcaaa tagcaagaca gatattcaat gatgtttatc caataaaatt ttatcatcaa
    26041 ttagtatcga gccaattaga tatggataga ttagattatc tgacgcgaga tagcttttat
    26101 actggtgtga gtgaaggaat aataaatacc cagagaatca tccatatgct aacactcacc
    26161 cctaatggag atcttggtgt attggctaaa ggcgtttatt ctatcgaaaa atttttaatt
    26221 gctcgacgca taatgtattg gcaggtatat tatcacaaaa ctacaatggc ggcagatgag
    26281 atgttatatc aagtaataag aagagcaaaa tttttaacta aaaatggaac cacagtaaat
    26341 gctactgaac ctttagaata tttcttaaaa tacaataaaa ctgaatttac caatgatgat
    26401 ttaaaagttt ttatgctttt agatgattat gatgttatga cagcattaaa aatgtggaca
    26461 caacatgatg ataaaatatt aagacttttg tcatctaaat tattaaatcg caatcttttt
    26521 agatgtttaa tattgaatga tatgccatct gataaaaata ttaatgagat agaaaagaaa
    26581 attttaaaaa aatttgcaat tgaccgagat gatttatctt ttttaatgaa tataagacca
    26641 atagaaaatt tatcatactc tttgcaagac aatcaaatta atttagtgta caaaaatagt
    26701 gaaataaagg atatatctca agtttctaat caactaaatt tggatgtatt gtcaatggat
    26761 acaacaaaat attttttgct ttatcctaaa gaaatcgaaa aataaaaatt taagttgata
    26821 gatattttaa tatttcatat tttaaaatga aatattaaaa tatgtaaatt aaggttatag
    26881 ccttcaaaat gagatatctt tgagattttt atattctttt atatgataaa atgtgctaaa
    26941 tgtaatttgc ttatatttaa tataaaaaat cttatttttt aatggatttt taaaaaataa
    27001 ttatctattt ttgcaaaaaa aattatgtct ataaaaaaat tagaagaaat tgttaaaacg
    27061 gtacaaaatc aaaagtccaa gaggcttgta gtagcttttg ctaatgatat tcatacatta
    27121 gaagcagtaa atactgcata taaactagga ttcattgaac caatattagt aggtgatgag
    27181 aaaaacatta aagaactagc taatcaaaaa aatattgata tttcaaaata taaaattatt
    27241 caagagtcta atgatatgaa tgctgctcgc aaagcagtag aacttattag aaatggcgat
    27301 gcagatgtat taatgaaggg tacggtaacc tcagataaat atgctaaagc aatattagat
    27361 aaagagcgag gcattatgcc tcccaagggt gttttaagcc acgttacagt tatagaaatt
    27421 cctacttatc ataaactatt attagttagt gatgtagcta ttattcctac tcctacacta
    27481 gatcaaaaag tagctatggc taattattta attcgagtag ctcataagtt aggtatagaa
    27541 aaacctaaaa ttgctgtaat cgcgccttct gagcaggtgc ttccagctat accatcttgt
    27601 gctgaagctg ctattttaag caaaatggga gatcgcaatc aaataaaagg tgcattaata
    27661 gatgggccaa tggcattaga tgtagctatt gataaagaaa gtgtagaaat taaaaaaata
    27721 gaaagctcag tagcaggtga tgcagattgt ttattatttc cctgtataga atctgcgaat
    27781 acatttttta aagcttgtac taaaatcgct cgtggtgaat tagctgctaa tgtgatggga
    27841 gctagtgccc ctgctatatt aacctctcgc ggtgatagta gcttgacaaa gacatactct
    27901 attgctttag cttgtttgat ggcagaaaaa taatcattta tggatatttc attgatttta
    27961 gttattaatc ctggttccac ctccactaaa attgctatat ttgatagtga tggcaataaa
    28021 atttatcaaa ataatattaa acattctgca gaagagctag cacctttttc taaaattact
    28081 gaacaatttt cttttagaaa agaaataata atagaagaat tgaaaaaaaa taatattgat
    28141 attaataaga ttaaagttat aatgggaagg ggagggttac ttaaacccat tcgatcaggc
    28201 ttatatgaag taaatgagtt gatgatagag catttgcgaa atagtataaa cggtgagcat
    28261 gcaagtaatc tcggtgcttt aatagctaat gatattgcta aatctatacc agatgctaga
    28321 gcatttatag cagatccagt ggtcgtagat gagttgcaag atgtagctcg tatagctggg
    28381 catccacttt ttaaacgaat tagcattttt catgcattaa atcaaaaagc tattgctaga
    28441 gctcacgcaa aagcacaggg taaaaattat gaagatttaa atctgatagt tgtgcatctg
    28501 ggtggtggca taagcgtagg cgctcaccaa aaaggcaaag taatagatgt aaatgatgct
    28561 ctcaatggtg aaggcccttt ctctccagaa cgtagtggta cactctctgc tggacaagta
    28621 gtagacatat gttttagtgg taaatatact aaggcagaaa taaaaaaaat gattactggc
    28681 aaaggtggtc tagtagctta tctaaatact aatgatgcac aattggtagg taaaataatt
    28741 ttagaaggtg ataaatatgc tgaattaatc tataaggcta tggcttatca ggtagctaaa
    28801 gagattggtg cttgtgctat cgtattaaaa ggtaaaatag atgctatttt gcttactggt
    28861 ggtattgctt ttgataatat atttacagat tggataaaag attatgtctc tttcctagct
    28921 cctatcttta tttatccagg tgaagatgaa atgttcgctt tagcttctta tggtatagct
    28981 tatttaaaag gagaagctaa cgttaatgtt tatggtgaat agcttttagt gcttattttt
    29041 tatcttttaa aaacactaat tatggttttt gtcttataaa ttatttattt taaatagttt
    29101 aaaattaaaa aatttttatg tcaaaatata ttattataac tggagcagca ggttttatag
    29161 ggagtgtgtt tgctcattat cttaataata atgaaataga taaagtaatc atagtagatg
    29221 attttagtaa aaaagaaaaa ttgttgaatt atgatacact accatatgaa agaatacaaa
    29281 gaaatctatt tattgattgg ttagaaaaat atcacaatga tgtagatgca gtgatacatc
    29341 tgggtgctcg tactgatact actgaatttg attacagcat ctttgaaaaa ttaaatttaa
    29401 attattctaa aaacatttgg aatatttgct ctaatcatgg tatagatttg tattatgcat
    29461 cttctgcagc tacatacgga gatggatcat taagctatga tgataatttg gaaatcattg
    29521 atagactaaa accattaaat ccttatggac ttagtaaaaa tgaatttgat aaatgggcaa
    29581 tcaagcaaat taatcaacca aaacattggg ctggtttcaa attttttaat gtttatggcc
    29641 ccaatgaata tcacaaaaaa agaatggctt cagtagtttt tcatggttat catcagataa
    29701 aaaatgaagg taaaataaaa cttttcaaat ctcataggcc cgactacaaa gatggagaac
    29761 aaaagagaga ttttgtatat gttaaagatg ttatcaaggt attattttgg tttctagagc
    29821 atcgtccaga taattctatt tataacctag gtacaggcaa agcacgttct tttatagatt
    29881 tagctaaatc tattttcgca gctatgaata ttccgacaaa tattgaattt atagatatgc
    29941 caacagatat tagagacaaa tatcaatact ttactgaggc aaatatggca aaattatata
    30001 atattggtta taataattcg ttttattctc tagaaaatgg tattaatgat tatgtaaaaa
    30061 attatttaat accagataaa tattacaaaa aatgaaaaaa attttatttc tcactttatt
    30121 tgtactaatt agtttttttt cttttggaca gaaactagta gattctacta aaacactttc
    30181 gttgatagca cattatagtt tcgacattcc tgcattagat atgaaagcca gatacggcaa
    30241 taatagtggt gtaggagcag aactgtcata taaaacttct aaaaatgtat tatttgcatt
    30301 agattacaat tatatttttg gtaataatgt aaaaatagaa gattctattt tcaaaggtat
    30361 atctactgct gatggattta ttttagatca aaacggtatg tatgcagagg ttttttgcta
    30421 tgaacgtggt ttttctataa ttgcaaaagc tggttatcaa tttcaactac ctacgaaata
    30481 ttattctggc attcaagtga tgggtggagg tggatttttg caacataaaa tcagaataga
    30541 gaatcgagaa aatgtagccc cgcagatttt aggtgattat gctaaaggtt atgattactt
    30601 gagaaatggt cctgctggct atctatatgt cggttatgtc tttcatgcca ataaaaaaat
    30661 tattaaattt actgctggtt ttgattgttt atttgcattt actcaatcta gacgagatta
    30721 tttattcccc atccaaggac ctgataatga tacacattta gacattattt atgggtttaa
    30781 agttggttgg ataattaatc tttataaatc acaaaaatct aataaaaaac aatattattt
    30841 taactagaaa gtacatttaa taatgaaaaa tattcctgat attggacctg ttaaaaaaaa
    30901 aatattaggc ataaaaattt cagaatctgt aatatttttt attgtttttg tagcggtatt
    30961 ttttcatata ttttttacac ataatagaaa aaatttagga caatgggaat accctatatg
    31021 ggctgatgca gctgcatatt ttgtttacct tcccgctaca tttatttatc actatgatgg
    31081 cagtaaaatg ccagaaaata tttccgcctt gactggtgat ggctttcaca tcgaaaataa
    31141 taagattgtc actaaagtcg ctatgggagt agctattttg caatctccat tttatgctat
    31201 ttcatatcta attgccaata tttttgatat taatgataat ggctttggga aagtatatat
    31261 ttatggatta ggtgtaggag ctgcttttta ttttacttta ggattattat ttttatattt
    31321 attccttaaa cgtagatatt ctaaaaatat atgtttcatt accctgctac taattatttt
    31381 tggtacaaat ctttattatt atacttatga gaaatttcta tactctcatt tatattcttt
    31441 ttttatattt tctgtttctt tgtaccttac agacaaattg ataaaaacta atttcaaaaa
    31501 gaaaatttat atagctttat ttggcataat cctatcacta gcttttctag ttagatacac
    31561 aaatcctatc tttatagtgt taatgctttt atttttagat atagatagct ggcaagcact
    31621 caaacaaagg ttcaaatata tttttaatat caaatttatt agcattgcag ccattagttt
    31681 tataataatc ttattacctc aaatgtttta ttggcattat ctaacaggta attttttata
    31741 ttattcatat ggtaatgaag gatttaattg gttagaaccc aaatttttag aatttttctt
    31801 ttcaccagat aatggagcat ttatttatac tccactttac attattattt ttatttatgc
    31861 cataataatg tcaataaaaa aggaacagaa tgggttattg atattaagca tatttttaat
    31921 agcttcatac atattcgcat catggtggtc atggagttat ggatgtagtc taggtaaaag
    31981 accttttgta gagctaacac ccatatttag catttgtata gctggtttgg tagataaaat
    32041 aatagaattc agaaaaccaa taattagcat aattttatca ttaattttat tattttttac
    32101 tttatttagt tttagcttaa atttacatta tgcaggatgc tcttttgtat ctgatacttg
    32161 ggattggaat gaatataaat tgatagtaaa tagaagcaga ttccctttaa ttagatttca
    32221 taaatatgaa tattctacag attttgagta ttttaataaa gagttatatt atccttttat
    32281 ttttaatact gatttaatta ctaaaactga tacagcatac tctggtaatt atgtatgcag
    32341 gatgagtcca gatgatttat ttagttttac ttatagacga gattttggca ctataaccgt
    32401 aaaagactct tgcaatgtag atttcaatat aaaatatttt ttcccaaata ttgattcaaa
    32461 taaacttaat gcatatttag taatacaagt agaagagatg gattcagcag tgtattatgc
    32521 atcacaaaaa ttagaatcta tacagaataa atggctagat gccaatataa atgtaaattt
    32581 accaaatttt ccaaattatt ttgtattagt tgcatatgta ctaaatgata gctcaacaat
    32641 actattagat gattttaaaa taagaataaa attttaaaat tgaaaatcaa taaaacgaaa
    32701 aaatagaatt cttttataat ttatactgct ttgttttttt ataatagtaa tattttcttt
    32761 tataaattat taattgataa aatgggaaat tttttttacg ggagaatttt tactcatatt
    32821 tttggttaaa tatcagatag aatctatgat ttatactcaa tactgaggca catataaaaa
    32881 attacttaat agtgcaagtg taattatacc taatattttg ccataatata agggagcaga
    32941 aattcataca aacagaaaat taacaaaaac ttattaatcc caaaatttat cagcctttag
    33001 ggatagtcat agtgatgaca ttttgctaaa tatcagatat ataacgatat tatagaagtt
    33061 aaaattttat tcaaatttac ctagtggcta taagcaatat ttagatgtac tagtctaata
    33121 tattcatttc taaaaatcaa cattgtatca tgttttcaat atcatttata aatggatgat
    33181 ttttaatatt ccgtttccct tttctgttat aattctgcaa aaatatatac cagcattgta
    33241 attttctgca ttccattcaa tagtatgtat tcctgcgttc atttctttat tgataagatt
    33301 aattatttta ttaccattca tattataaat atcaattata acattagtat tttcttttaa
    33361 ttcaaattta atttttgttg ttgatacaaa tggatttggg aaacatgaaa aagagtcaaa
    33421 gtcttctatt attgaattgt taacagcatt aatgttattt gtatccactg cgatattata
    33481 aaccatacca ccatgtccaa atattcctgc atgtactgat ttacctgatg caatgacagt
    33541 agcaccattt gaagaagttg aacaagagaa aaacgagcct ggtgaattaa gagaaaaaat
    33601 tggaatagcg gattcagtat taaaaatgag caggtcattt attaaattac ttttatcacc
    33661 ccaggagcat gctgataaaa tattaccatt ttttgaaaaa gaaacagatg ttactatatt
    33721 tcctgttcct gtatatgtcc attgaggagt agggcctgat gtggttttga atagcaaaac
    33781 tttaccatct tctgtagtat cggttgggaa attaagtgta ccacatgcaa taaggcttcc
    33841 atcataagaa acatctacag ctgacatcca attataataa ttagtgggtg gttccattct
    33901 ttgccacagc aatgtataat tagaaccatc ccagtgataa acctttaacc agccatgaag
    33961 atctattgtt gcaataatat ctccattacc acttattccc atggaatttt gacatccatt
    34021 gattgtcgta ctgaaacgtt caatccctgt gaatgtctca tacactgcaa cttgattata
    34081 tgaatttact atcacaatag agtcatttcc agatatcttc ataccatagg cagaatgata
    34141 aattttatgt ttccatattt cggatgttga gcctgactga aatccaaata cccagcatga
    34201 atcagaatca cctgcagaag cagtagcaat aataaaacta ccatctgaca ccatttcaat
    34261 aggtccacca cttatggcac cgggaatcac atttaaaata aattcattta caacatcccc
    34321 tgtaatttta tctaatacat atattttatc tggagaacta atagcaacta aattcccatt
    34381 acgagacata gcaatagaat ttactaatgc agggaattcc cagagtggaa tgctgtcatt
    34441 attattataa agagctgccc tgcgattatt tatatcccag ttaatcaatt gataaaaccc
    34501 agtattatca gtcctacata acgtaccggt agaagtagca tcaatataac gccagcgaac
    34561 aaaagcattt attgaatcct gtcggcttcc ctgagcttgc aatatgcagg aaaaaataaa
    34621 catagaaaaa acaaaactta ttaaataaac taaaatatta tttacactat tagctatttc
    34681 aaatgatatt ctttttgaga tacaattttt tgtttccata aatattattt ttatgtaaat
    34741 ttacattaaa atttttaatt acacaataac gaataaaaaa atttttttgt aacgttttgc
    34801 ggataaaaga agttgccgaa ggcaatttga ggaaattttt gatttccgtt tatccgctgt
    34861 tatatgaccg agccaaaggc gaggcaaggc acgaaatgcc gaagcgtttc accctttatt
    34921 catatccatt tcctatgact ccctaattat ttttaaattt ttcttattct ttcagttgtt
    34981 actaaatact ttctgacttc tcttataaac cttgcccaat ctccgtctgt gtccaagaaa
    35041 aaagccagtc tccactacaa gcgtttttag caatatctgt ctgatgagca ttttcagtgc
    35101 cattaggata tttttctgaa ttccattcag aaaaaattaa ttttattttt gaactattta
    35161 tactttgaat taattctaca gttttatcat catcgcagtc acttatattt acaataaatt
    35221 catctacaat aggtagaatg gatagaatgg actgtttaat aggataatac agcttatttg
    35281 catttttgca aaaggtaaaa ccactgattc gcatttttta atttcaaaat taataattaa
    35341 atagaaatag gtttttaatt taataaaaat ataagtagaa tgaatggtag tgaggggtgg
    35401 ggtattatga tagagagaaa aacaagaggt ggcatagttg gctgttataa aaattttaaa
    35461 aatttttata acaaatatat aacaaataaa aatgtttaat tttcagtatt ttataatttt
    35521 attaattttg taaaaaaaat aatatttata tcaattaaaa aaaattttta tttttatata
    35581 aaaacaaaat taaaattatg gcaacaaaaa ataatgacaa tattaaatgt tcattttgcg
    35641 gacgcaatgc ttcagaagta gatttaatgt taagaggatt caatgctaat atatgtaatt
    35701 tttgtgtaga agaagcaaaa aaacaaataa atctgcaaag tacaaagtct aaaaaaactc
    35761 caaaattaaa attaaaaaaa cctacagaaa tttatgaatt tttaaatcaa tatgtgatcg
    35821 gtcaagacta tgccaaaaaa gttttatcag tagcggttta taatcattat aaaagaattt
    35881 taaatcctat tataaaagat gtagaattag aaaaatctaa tatacttcta atcggtgaaa
    35941 ccggaactgg taaaacttta ttagctaaaa caattgctaa attactagat gttccatttg
    36001 caatagctga tgctacagct ttgacagagg ctggttatgt tggcgaagat gtagagacga
    36061 tacttactag actattgcaa aatgctaatt atgatgttaa agccgcagaa atgggaatag
    36121 tatacataga tgaaatagat aaaataagta gaaaaaatga taatccaagt ttaacaaaag
    36181 atgtgggtgg cgaaggtgta caacaagcat tacttaaaat tttagaaggc tctgtagtat
    36241 ttgtacctcc cgaaggagga cgcaaacatc ctgaacaaga atttattcat ttaaacactt
    36301 ctaacattct ttttatctgt ggtggcgctt ttgatggatt agataaaatc atagctaata
    36361 gagtgaaaac ttctactatt ggtttcggtg tatctcaaaa taatattaat aacggcgaaa
    36421 aaaatatctt acattatgta actcctgcag acctcagaaa atatggatta ataccagagt
    36481 ttataggtcg tatacctgta attactactt tagattcatt aaataatgag gaactaaaac
    36541 gaatactaac tgaacctaaa aatgcgctag taaaacaata taaagcatta tttgaaattg
    36601 ataatattga attagaattt acagaagatg ctctagatgc tattgtagaa aaaactattg
    36661 agcttaaact aggtgctagg ggtttaagat cagtaatgga aaaaacaatg atggatctaa
    36721 tgtataatac tccacatatg caagaaacta aaatattaat tactaaaaaa tttgtggaac
    36781 aacatttgca aggttttgat aatgaacaat caaaagttgc atgaaaccac gttgttttgt
    36841 attatgtatt ttattatttt cttttttgaa atttaatgct cagaataatg atgatcaaat
    36901 attgtcaagg gctattatat accaaggcga taccttacct tatgtagctt tagatactgt
    36961 aaagtattat tatttcgatt tcaaaggttc taataaagaa tatatagcat ttaccaaact
    37021 agtgagagat gtaaaaaaag cttatcctta tgctaaacta gtcgcgaata aagttaatga
    37081 aattaatgca tatttaaata ctataaaaga tcctaaatta ttagaaaaag aaaaaaatag
    37141 attagaaaaa gaattaagag ctcaatatga gaaacaaatc cgtaatctca cacgcactca
    37201 gggcaaaata ttgataaagt tgatttatcg tgaaactagt aaaacttctt ataatctgat
    37261 aaaagattat cgtggaagtt ttctctcttc tatttatcaa ggaatagcaa aaatttttgg
    37321 tttaaacctt aaagatactt atgatcctga aggtaaagat cgcaatatag aaataatagt
    37381 gaaactcatt gaaaatgggc agttataatt gatattataa aattaaaaat aataaacctt
    37441 aacttcattt atagaatata aaaataaaat acacaaaaaa gtatttaaac tattataatt
    37501 tttattattt ttgctaaaaa aatgaagaat ttttttcttt ttagtattct attctatatt
    37561 tcaataaata tattttcaca gcaagaatgg tctttggagc aatgtattca atatggagtg
    37621 cagaatagtt taaatattaa aaaccaagca ctgaatgcag agattgccaa agctaaccat
    37681 ctacagagca aactagcgtt attaccagat atccatgctg cagctactca tagctataat
    37741 tatggcaaag tcgtagatcc ttttactaat gactttgcaa tggatagaat acaaactaat
    37801 aatttttatt tagcatcttc aatagtactt tttaatggat tgaaattatt aaataatatc
    37861 aaagagcaga tgagcaatac taatactgct gagctattat tgaaaaaaac tgaaaatgat
    37921 gtgatgctaa acattgccac actgtatctg caagtgatat ttgatagctt aaacataagc
    37981 actatacatc aacaagtaat tctatcagag ttgcaagtaa atcgagctga attactctat
    38041 gagagcggct ctatctcaga agacaattta ttaaatttgc aagcacagtt agcctctgaa
    38101 caatctaatc taataacagc tgagggcaga tatgagcttg atttgttagc tctcaaacaa
    38161 gctatgtatt acaatgacac tgtaccttta aaaattaaaa ttcctacaga tctacaaccc
    38221 atcatagata agcacgtcaa tattccaccc ttagatacta tacttttata tgcattcaat
    38281 aatttaccgg atatcaaagt tgcagaatgg caattgcagt cagctaaata ctcatttaaa
    38341 gctgctaaag gtaatttttc tcctaccctt tcattatcac tatcgtatgg cactggttat
    38401 tctactgctt ctaagcaaat taccgaatat gaatatagtg gttatgatac tataggtatc
    38461 actactggca ctccaccaaa ttttgtactc gctcctcatc ttaattataa ttatcaaact
    38521 atccctttta aaaatcaatt aaatgataac ataaatcaat ctttggggct tagccttcag
    38581 atacccattt ttaatagagg tatataccat aatcaatatc agattggcaa aataaattta
    38641 caaatagctg aaaataattt agaacaaact aaattaaatc tgcaaaacat tatagaacaa
    38701 gcttattatg atatggtagc tgcagagaaa aaattagatg ctagaaatct ttatctaaaa
    38761 gctcaaaaga ttgcttatta caatcagcaa gaaagattcg aacttggcct aattagtagt
    38821 atagatttta tgaatagcaa aaatcaatat acacaagctg aaatacaaca gttacaagct
    38881 agtgtagaat tactttttcg ttatataatc ttagattttt atcaaggtaa atcattatca
    38941 ctatgaaaaa taataaaaaa acaaaacgta ttttaataat atcaggagtt tctctattag
    39001 tattagtaat aattttaaga gctattggtg tgataggagg tagcaatgga ttagaagtag
    39061 cagtagagaa aactatacgc agaaatatta ccgaaactat caccgctagt ggcaagatac
    39121 aaccagtgaa agaagtaaaa ataagtcctg atgtgagtgg agaaataatt gaaatcttag
    39181 tgaaagaagg tgataaagtg aaagctggtc aagtattagc taaaataaat actgatattt
    39241 ataaagctac ctacgaacaa gcttctgcgc aattacaagc tcagaaagct aatttagcta
    39301 atactgaagc tagactaaag cagacagaag cccagctatt aaatgctcaa ttaaatttca
    39361 atcgtcaaaa acaattatat gaaaaaggag ccatctcttc atctgaatat gaaactgctg
    39421 aaactaatta caaagtagct aaagcagaat atgaggctgc taaacaaagt gtaaaagccg
    39481 ctgaatttac cgtggctagc gtagaggcat ctatggatga agctcttaaa aatttgaatc
    39541 gtactactat tatagcaccc ctttctggtg tagttactaa actaagtgta gaagtaggcg
    39601 aaagagtggc aggtgcttct caatttagcc ctggtacaga ggttatccgc attgcagata
    39661 tgaatgaatt agaaatagta gttacagtta atgaattaga aattgtacgt attcaccaaa
    39721 atgatacagc taatattaca ctagatgctt atgatgatta cactttcaaa ggcattgtta
    39781 cagatatggc tatttctaca attaatactt tatctcttac gtctgctgat gaagtgagta
    39841 cttatgaggt aaaaataaga ttattaccat catcttatca aaatctttta acaaatactg
    39901 aaaccccata tccttttcgt cctggtatgt ctgcttatgt agaaataatc accaaaaaag
    39961 cttttaatgt cattgcagtg cccatagctg ccgttactgt gaaaactgat accgctacta
    40021 tgaagcttaa tgaggtggtt tttgtagtta ataaagataa taaggtagaa aaaagaaatg
    40081 ttaaaactgg tatccaagat aatcaataca ttgaaataat agatggtata aaagaaaatg
    40141 aaaatgtggt tatagctcct tattcattaa ttactaatat tttaaaaggc gatgaatctg
    40201 ttactattgt tagtaaatct aaactctacc agaaatgaaa tatatcttaa taattgaaac
    40261 ttcagacaaa gctaattcta ttgctatatg tgatagtaat ggaaaactga taaattataa
    40321 agatgaaatt tccgataatt ttactcacat agaattgcta catgtgagta tagataaatt
    40381 atttaatgaa tgcggcatag ctttttcaga tttatgtgct atagcagtga gtagtggtcc
    40441 aggcagctac actggactac gtataggtgt gtcttctgcc aaaggctatg catatgctct
    40501 tgatatccca ttaattagct tacctacttt agaattgttg gctaatggga tgatagatca
    40561 tatagatgaa aagaatgaaa atatcatttt gataccaaca atagatgcca gacgtatgga
    40621 agtttacatg caaatgtttg attatcaaat acatgctatc actgaaccat ctgctgaaat
    40681 aattaatgag ctgtattttt ctaagtttct caaagataaa atatactatt ggggtgggtc
    40741 aggtgctgaa aaattatcag attttttccc tgataatcat aaattagtac atcaggtgca
    40801 atatccatta gctcgttatg gtgtttctat agctatagat aaatacaata cccatcaata
    40861 tgaagatata gcatatttcg aaccttttta tctgaaagaa tttattccag gaaaaccacg
    40921 tgtgaaagga ctagaataaa gacttagtct ttaataaaaa ataaaaattc taagtttttt
    40981 aaataaattt taatagtaaa gatataaaca ataaaagcat agtgaatata aatgagactt
    41041 attattctat ttcattcctt tgtctctaac tcttgggtct aatattagat acaataaatc
    41101 tactattaaa gttactataa taaataataa tgcaacaata aatattcctg ctttcaatac
    41161 aggcaaatca taattttgta agctttctac tatcagactc cccatacctt tccaattaaa
    41221 aacatattct acaaatactg atcctgcaag taacgatgca aaccaaccac tagcagatgt
    41281 aattaatgga ttgatggctg atggtaaaac atgatggcac attatattgt aacttgatag
    41341 tcctttagca tgtgcagttt ttatataatc agtatttaat acttctagca tagaatttct
    41401 aattaatggc acaaaaactg ctaatggtct gatagataat gtgaatgctg gtaatatcaa
    41461 attttgccat tttacatatg tactatacgt taactcattt acttctatta ggttgccatg
    41521 catagataag caagtaatat catgtaacaa ataaccaaaa atccaagcta tcaataaggc
    41581 tgcaaaaaat gaaggcaaag acattccaat agagctaaaa actattatca acctatcaat
    41641 ccaggtattt gcttttaacg ctgatatact tcctaaaatt atagataaaa ccaaagctac
    41701 aagcattgtt actatagcta aaattaatgt taatggaaat gcttccttta ttagttgcca
    41761 tacgggctgt ccgctgatat aggattcgtg aaaaactatg ggtttaaaac ctaagtttac
    41821 tttatttaat ttaataattg ttaaggattt ctctgtataa acattatcat aatagaatgt
    41881 attttctcta ttatttgaat aaaagccaag cggactgata tcgttcaagt attttacata
    41941 ttgaatataa agtggcttat ctaaacctaa tttttttagt atcgcttcct cagtagctac
    42001 atcactcctt tgtcccataa tcaatctaac aggatcagaa ggtaataaat taaataaaat
    42061 aaataaaaaa gttaatactc cccatattaa aagtattata tagcctaact ttctgaataa
    42121 taacattgtt tctgatcatt aatagattac aaaataaata aaatattttt attattttaa
    42181 attaaaataa tggtgattaa tttttttata gctattacat acaattaata ataatttaat
    42241 aataattttt tttagaagat tctgtataaa tcacaatgct tatgtattga gtagataaag
    42301 tttaaaaact gttataaggt tttgtaagaa gtgcttaatt gaaagtaaac gtattttgaa
    42361 attatctttt gttttacttt acgtatttta tactaccatc aaataatttt tatttaattt
    42421 tacaaaaaaa taattatgga taacttcaat ctattagatt atcaagctct acccaaagag
    42481 cagaaacgta gatttctaga taatctatat caatttttat tagaaaacaa ctacacagcc
    42541 gtagattatc agaagctaaa aatacaaagc acagccccca tctgtcctag atgcgaaagc
    42601 gaaaatgtaa taaaagctgg agtacgcgac ggcaaacaag tgtacaaatg caaagattgc
    42661 ggccgacaat accgcgaaac agctagaacc ttcgtatatc gcatgcgaaa agctgataaa
    42721 atgctcgatt acctaaaatg catgttagaa ggcaaaagtc taagagcttg cgcatcggaa
    42781 gtaggcataa gtctgagaac gagtttcaat tggcgacaca agatactagc agcgatcaaa
    42841 tcattacagg gtggtatcag cttctcaggc attgtagaag ctgatgagct gctaatgaaa
    42901 tattcagaaa aaggtcgcaa atacagaagc cgaaaagaat acgaaaaagc aaagaaaaag
    42961 aaacatccca aagtagctgt gctagtaatg acagacaggg aaggcaattt acttttcaaa
    43021 cacgtaggac gcaaaaaagt aaccaatagt cagataagag aagagctatc gcatagagtg
    43081 acagatagca acttaatatg tgtgaagcaa aagaaggaat tcaaaaaagc tgtgaagggg
    43141 accaaaagta aaaaagttgt tgtaaacaaa acacagatta gacgtggtgt ttacagtgta
    43201 caggtagtcg ataagcatgt aaatgatttc aaggtttggt tgcggcgatt tagaggtgtg
    43261 gctactaaat acctccagag ctatctgatg tggtttgtaa tcacacataa atacttaaca
    43321 agtgagatag
//
LOCUS       DAOPRN010000010        41501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_720_length_41501_cov_111.734474, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000010 DAOPRN010000000
VERSION     DAOPRN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 41501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41501
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_720_length_41501_cov_111.734474"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..707
                     /locus_tag="PKI65_02135"
     CDS             258..707
                     /locus_tag="PKI65_02135"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOB77273.1"
                     /translation="MSYIRISKEFHFEMAHVLDEYDGLCAYVHGHSYTLIVTVKGKPN
                     TDKNSPKYGMVIDFNTLKSIIQKEIIELFDHSLIVNKNAEWLDKVENIKSFSRIIKLP
                     FQPTSENLLLHFIDIITPHLPPEVTLVQVHLRETNSSYVEWLYEDNK"
     gene            767..2620
                     /locus_tag="PKI65_02140"
     CDS             767..2620
                     /locus_tag="PKI65_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOB77274.1"
                     /translation="MKKLLKQFFYFLKLTSIKIFEWFYKVNPKILRFCNIILSFLSIF
                     AGLLTIFLLGNLYTPTVGHNLEPYIDFISWSISIIYLIQIILLILPGGKKFSWSNLLL
                     KMIFIITINYFVYFIPDFISPYLHDVGYIHYYKLFLIFLVFIRTISLLAQNLYKISIH
                     PAKIFIISFIIIIIIGSLLLLLPAATYRDIKYEDSLFVTVSAVCVTGLTTVNIASTFT
                     WFGKLIIIILIQLGGLGIMTITSFLALSFTRTDLSIKEQQGLEFNLGSSSFREIKKNL
                     YAIILITALFESIGAILIYFSVPDIYFDNLAQHIFFAIFHSVSAFCNAGFSNLPDGMM
                     NEVFVNNYLSIFTISGLIIFGGIGFFVINSLLKNINFKFKNLIRKIFNKPKIIKPKII
                     KPKIININNLIVTRTTLILIILAIAVILILESNNSLISYNWFDKVVQAFFCSVTPRTA
                     GFNTLDFASFTPATIMFIIILMWIGASPGSTGGGIKTTSIALAFLNAISIGRNQDKIT
                     FKNRTISNITVQKSFVIIVFSLLTIFIGIIFVCAFDPHLGFMNIMFEVFSAFGTVGLT
                     LGITPNLSLASKIVIIIEMFVGRMQFVILFLAIIKRAKRRLIKYPEIDLYL"
     gene            2631..3347
                     /locus_tag="PKI65_02145"
     CDS             2631..3347
                     /locus_tag="PKI65_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOB77275.1"
                     /translation="MSKKVLVLGLGNFGSELAKRLTHEDIEVTGIDIDHNLVDLHKDS
                     ITNVVEGDATKDQVIKELEVSMYDFCIVCIGHDIGVSLTMVALLKKYEANIIYARAIN
                     AIHKQILSLLRVKYIFEPEIESAGRLAMQIASRMVFDTFPISKNYIAVEIDVPKSYEN
                     LKLEDIKINEEIDVLMLKRYSYVENPFERSELYHPPAYEYTPHKHGKTLIHQNDRLVI
                     FGPVEKIRNYIKIYDKENQV"
     gene            complement(3368..4246)
                     /locus_tag="PKI65_02150"
     CDS             complement(3368..4246)
                     /locus_tag="PKI65_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77276.1"
                     /translation="MSWQLINRYNINENKWNEVVSLSRSRHITGLSWYLDIVSPQWYG
                     LVWDDYQIIFPFAKKTKLFFPYFLQPPMITYFRPFSLIDINGEIFQSLISVIKKKVIF
                     ADFYIDDLGFKSKYISDCRINYLLRLEDIEDLRSMMSEHHLRKIKKMEKNEPCLIQLT
                     NVPTNFLNDFLCNSPYMLNETELRNIIIKLGKNIEQYQLGGWWGLKIKDTIMAIALIS
                     IYHDILVFHSFTANDKGRKSGATHYLIYKIIENFKKSNGKIIDFDGGNEEKMAFFYQG
                     FGGKPYKYLELKIKKW"
     gene            complement(4243..5541)
                     /locus_tag="PKI65_02155"
     CDS             complement(4243..5541)
                     /locus_tag="PKI65_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOB77277.1"
                     /translation="MTPVIIASDFISERLKYTLDFIFKTVLEFPYILINDLDAFAHIR
                     YSTKPSTESINIFKISPLLNDNFLTKEIPTTEKSDNHLSLYPINQENYFNQDIFSAVF
                     YCISGYERYFNKYFDIHGRFIPENSTIFHRGFPFIYPWVDKWIFLLAKELKTRFKDLP
                     IQFEKIYKLQVTIDVDIAYTYLCKGFYRNIGGLFRDLLNLKFLDVFNRIATLMQIKKD
                     DYDHFDLLLEIGKYLQNPLIFFFLLSNKTTKFDKNNHINCRRFLNLIRTISNKYHVGI
                     HYSYYAKDNPDLIKLEKTTLEKIINEKIKIGRAHYLRLDMPDMNNLANIGIKEDYSIG
                     YASLPGFPLGTSHPLYYYDLKGEKITNLLLHQTSLMEGTFVDYKKSTVDDFKNTFEQL
                     KSSLVLGGEIVLLFHNESFTNKNRWQGWTPFFIEFLKNIK"
     gene            complement(5544..5939)
                     /locus_tag="PKI65_02160"
     CDS             complement(5544..5939)
                     /locus_tag="PKI65_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77278.1"
                     /translation="MKSQDPEFSKFLEESFKVYRWGARISLVTLVFFLFNRWWGIAGK
                     EWWAVLIALFIYGTIMPFIQPSHWKFKQKISHGMFAYLMLHLDMFIYCWLISRLKLKY
                     FVPFLPQLLFPFGWTLIFQLVVSGIKTKK"
     gene            complement(5954..7087)
                     /gene="wecB"
                     /locus_tag="PKI65_02165"
     CDS             complement(5954..7087)
                     /gene="wecB"
                     /locus_tag="PKI65_02165"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOB77279.1"
                     /translation="MRICNIIGARPQIIKAAALSRTIRNLYKEEIEDIIIHTGQHYDA
                     NMSEIFFDEMDIPQPHLNLGIGSASHAVMTGKMLEKLESVFIKYRPDIVVLYGDTNST
                     LAGAVAAIKINIPIAHIEAGLRSFNKQMPEEVNRIVCDHCSTLLFTPTITGLRNLQYE
                     GFHLDNEPPHSADNPAIYLSGDIMYDNSIYYYEKAMISSNIIEKLKLKDKKFVIATIH
                     RNTNTDDPKRLKEILEKLNAIVENLNISVVFPLHPRTKKLIKSFELKYLLDKIIVTEP
                     LSFFDMIKMENFASLILTDSGGVQKEAYFYQKPCIIMRRETEWVEILEAATGALEPQN
                     IDDFLSLANSFISNPPTNFPEYFGNGQTAKFICEKLLNYAKNK"
     gene            complement(7163..8452)
                     /gene="tyrS"
                     /locus_tag="PKI65_02170"
     CDS             complement(7163..8452)
                     /gene="tyrS"
                     /locus_tag="PKI65_02170"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOB77280.1"
                     /translation="MDFIAELEWRGMVYEMIPDVREYLSQSGCTAYVGIDPTADSLHI
                     GHLVSIMLLRHFQRAGHRPILLLGGATGMIGDPSGKSEERNLLAEEIIRKNEEGIKKQ
                     VTKLLDFNDKKNGALLLNNYDWMKDYRFLDFIRDVGKHITVNYMMAKENVKRRINGEV
                     GEGLSFAEFSYQLVQANDFLHLYKNYNCKLQMGGSDQWGNITTGIELIRKVTGAEAYA
                     ITCPLITKADGNKFGKTEKGNVWLDPDKTSPYEFYQFWINTSDEDAEKYIKIFTELDK
                     NTIEAIIAEHHMAPHNRILQKKLAEEVTFMIHGVQALEIAKNTSEFLFGDGSIDILKN
                     LNDDSIFSIFAGIPKYNISSESIASGINIVDLLAVSTKIFNSKSEVRRMIANKGLYLN
                     KEVVDNENLIIDANNLIKNKFLIVQKGKKNYFLIIAE"
     gene            8856..9212
                     /locus_tag="PKI65_02175"
     CDS             8856..9212
                     /locus_tag="PKI65_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77281.1"
                     /translation="MNNIKIKGIIKKLKKLKIRKKYTQNSLSRLINNHCSVIAQIKLD
                     ERNIYTKELKQLAYISTISDDKLKLFDYIVDKILLSDKVNKAENQIKFIENSHKSKKE
                     NYQFNYKTINLYKEAK"
     gene            9212..10843
                     /locus_tag="PKI65_02180"
     CDS             9212..10843
                     /locus_tag="PKI65_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOB77282.1"
                     /translation="MDISREGIYNLLLDLVKIPSVSPGKGETLIAEFIHDTLRDLPYF
                     QKHTDNLYYIPIPNDPFSRKNVFAMVEAEPKTNRTIILTGHIDVVDTKEARSLEEVAF
                     DPEEYTKRIKELDLPEEAAKDLESGKYLFGRGVMDMKAGIAIQMALLAEYSQNPKNIP
                     INIAFLAVVDEENNSAGMRTAITHLAKLQNQGLEFVACINSEGVIPKYPGDTNRYIDI
                     GTIGKIMPFFYCVGRESHVGQYYAGLNANLLTSAVNMILEGNPEWAEKWRGEVYPPPA
                     SLKQKDLREIYSVTLPARAMVYFNHLTVTSTPAEILEKSKEVATQAFEKAINHLEQSA
                     NEFSKKANTSVPIPWKTKIITWQELLIEVSKTFEGELDNYIKEFINNLPDELDERDKS
                     LATVGELIRLYPDKNPMIVVGFLPPYYPHKTNKREIESEKKMLAAVEEIIKEAKDEFG
                     EILAISEHFASISDLSYLGFQGEKDELVPLALNTPGWGIIYDIPLDDLLLLDIPVVNL
                     GPMGKDAHKFVERLELDYSLDVVPKLLKSLIQKLS"
     gene            11006..13828
                     /gene="uvrA"
                     /locus_tag="PKI65_02185"
     CDS             11006..13828
                     /gene="uvrA"
                     /locus_tag="PKI65_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOB77283.1"
                     /translation="MENFNYREILIEGARANNLKNITLSIPKRKITVVTGLSGSGKTS
                     LAFDTIYAEGQRKYVESLSAYARQFLGKIMKPDVDKITGLSPAIAIRQFSTNVNPRST
                     VGTITEIYDYLKILYTRIGKTHSPISGREVKHYKVEDVVEYVMSFPDKSRFFIVAPYI
                     EMVGRDVEEQLGLWHINGYSRVLLDNNIYLIRDLLNDNNKLTKISSDKLFLLVDRLTK
                     SDDEDFAFRIKDSIQSAFFEGDGKIFIVILSGKERKWVEFSNKFELDGMRFSIPDVNF
                     FSYTSPYGACPVCEGYGTVVDIAEHLVIPDPTLSIYEGAVAPWRGEKMSEWQKYFIAY
                     SKKYNFPIHKPYKDLTEEEKNLLWNGRDDLEGINSFFKMLQENTYKIQYRVMLSRYRE
                     KTICKACKGTRLRKETQYVKIHGYSLPDLLMMQIDQVRDIINTWVITEKEREKVKYVI
                     KELNSRIDYLIQVGLPYVTLNRGMNTLSGGEIQRVYLSRALGSTMTDTLYVLDEPTVG
                     LHPRDTHKLIDILKKLSKAGNTIIVVEHDKDIIKASDWIVDLGPKAGSEGGNVVFSGP
                     IDQLSGDPNSFTAKYLYDPLPAIDSKTKFSFLETNNRYIELKGANKNNLKNINIRFPL
                     NQLIAIVGVSGSGKSTLVVDELYEEFNKYLQHFDFEKTKLSGDFKLIKDIVLIDQSSI
                     SRSTRSNPITFLKIFDYIRELYSLMPLSIQRGYKPSTFSFNTTGGRCETCKGDGYIII
                     EMQFMADVKITCEDCHGKRFKEDVLEVTFGQKNIYDILQMTIDDAYEFFKSQYSFSKN
                     KKIANLITQTLNGLELLKKVGLGYLKMGQSTDTLSGGEAQRLKLASYIGAGEKTEKTL
                     FLFDEPTTGLHYYDVDMLYKSFKELIMKGHTICFIEHNIELIKRADWVIELGPDGGEQ
                     GGYLIFEGTIEDFIKTDTITTKYLKD"
     gene            complement(13948..14649)
                     /locus_tag="PKI65_02190"
     CDS             complement(13948..14649)
                     /locus_tag="PKI65_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOB77284.1"
                     /translation="MKKKILLNVILVTISIVTFAQMPTGTNPFSIEGGLTINIQTGST
                     FAAPSLKLRYFVNDKISTRLGLAINSTKSIDKIYGLNDQGLPVESKLGTYEEKDFSTQ
                     ISLGAAYHFSIKEKLSPYAGLDFAFLTGSYTETWKNTDQNLTGYDDSKNGHYKYKYGG
                     WGFMIFTGVDYYFAENIFVGAEIGIGMMSYKDKNGEWSQTVGQTTTTVDIFSTFSESY
                     FGNMASTMIRLGWRF"
     gene            complement(14855..16312)
                     /locus_tag="PKI65_02195"
     CDS             complement(14855..16312)
                     /locus_tag="PKI65_02195"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOB77285.1"
                     /translation="MKKKYLFILVTALSINIYGQYNFTTNDIPVIPQSTYLNPALMPT
                     CRVYIGLPLISSNSIYIAHTGFNPYQVLETRNDTTFINVNSVVNKLAKKNYLFQNNNI
                     ELLSFSFKIKNKHYFSFNIMEKINTWVSYSKNLVEFAYKGNGALLDKSITLNPALDFS
                     YYREYALGYAMNINDKISVGARLKLLSGKININSGNTDIKLITTGEMYDITAIGTLEL
                     NTSMPEEMRDTNYINNFDSLVDNTDFVKDFILLKNNLGGALDLGFTFKPIDNLIIGLS
                     IIDLGFINWKNDPLNFINRNENASFTFTGVDVAPLIAGSDSLFDERLDDLLDSIKDVF
                     DIDSTYNSYTTWLPTKIYLTASYDITPKTTMGVGVHLMFLNKSVYPAFHFKALHKFSH
                     IFQASINYTIANRNYTNLGFGFALTLSPFQFYLTTDNILGPLIYDEYQYYDEGDNKFK
                     YVNVPHNLKYLNVHLGINLVFGCKAKKYAEPSIRF"
     gene            complement(16328..17920)
                     /locus_tag="PKI65_02200"
     CDS             complement(16328..17920)
                     /locus_tag="PKI65_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77286.1"
                     /translation="MKNFFLFVSLMGFIIFFVTSCVKDEFNVDNIATSDWNPNVAAPI
                     INSNLYMWDLMNDYDSSEVFEEYNTNLIYLLYQGNIATNTMENYINIPSQNQSFSQLT
                     INIPGGNLSSDYEVTHNYSFTLSLPNNMLLDTMILKSLDLSLNINHNINYPIDVDFSI
                     NNATVNSSPHNENFSVPSLSYDYSKHYNNIKLRLGANNQLSFDIKINIHGDGSANLSP
                     YYFILSIGLNNIGFSKLFGYLGDYNLNLDSDTIKIKLYNNHFDGIINWEDPKIFLTTT
                     NSIGVPINININQIKARRENLPSSSMTITGMPPIWSLNYPSLSEIGQNKLSFYQITKN
                     NSNIVSAFNISPNYIDVVADARINSNGHSNFVIDTSRLHLNGRVELPFHGTARDFVLI
                     DTLELDLGDSTYFKHVDWFMFKIFTTNYYPVDADIQVYLLDAQNNIIDSILPPNQRIA
                     ASGIVGPAPDYFVISPSTKLATNKFEGARKDALVNVRNAVIKAKLNTTDHGNNIVKIY
                     SYQYINLRVSAQAQLINPYPFN"
     gene            18015..18917
                     /gene="menA"
                     /locus_tag="PKI65_02205"
     CDS             18015..18917
                     /gene="menA"
                     /locus_tag="PKI65_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOB77287.1"
                     /translation="MSLKKWLIAVRPWSLAASTVPALMTITYVYFLKIEYSLEINIFY
                     GFLALIGAGIFQASSNIINDYIDYKHKVDRLESLGGGRTIIENIMRPKSFIIYGYILL
                     IIGIILGLFLTFHTGYHLLWIGAIGIFFTIYYTKFKYIGLGDLVIFIIYGPMIGLGTA
                     YVMTNNLLWEVMLINVPIALLIVDILNANNIRDIIFDKKAKIKTPAMLLGLEKAKVYY
                     IVLMIAAYLFTALLIFLNLLHPLCFLVLLSLPIAIKNFNIINHATIETLEEIKHLDAK
                     TSELVLVYGILFSISNFIAGFFII"
     gene            18972..20510
                     /locus_tag="PKI65_02210"
     CDS             18972..20510
                     /locus_tag="PKI65_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOB77288.1"
                     /translation="MESRPDVRELIIRAAETFGKQTAFIYNDKEYNFLDLKENSFRLA
                     NSFLDLGYKKGDKIAIYLPNCIEYIYAYYAIYSIGCVVVPIDFFLSQNEVIAIATHCE
                     LKGIITTTEAKFNLLELRQSIPSLKDIITIEDNSKFLSFWRLATNSKPDLDDQGITTN
                     MISSIFYTSGTTGMPKGAAWNYLHIHLGAEQYKYFGTYSGLEDRMRVDERERLLAPIP
                     FSHSGGVLYPMMAIKFGVSTVIMPQFNPYEFVRLVDRWQVTGFHMVPPMYYAILYLKD
                     IKKYKLESLLWAAVFGAPSSAELMMRFERLCPNAVIVNGWGMTEVIPPTSLSTPGNIR
                     SIGKPTPNVELKIFDNHGNEVKIGEVGELVVKGKSVFLGYYKEPELNNEVFKDGWFYT
                     GDLARKDEEGNYYIIGKSKDTIKVGGELVWAAEIEELLLKHPCVKEAAAIGIPDHLRG
                     EIVKCYIALREGVVFPKQYLLEYMRENLAKFKVPREIEYLDELPKTGSGKVNKAALKN
                     LNKN"
     gene            20514..21563
                     /locus_tag="PKI65_02215"
     CDS             20514..21563
                     /locus_tag="PKI65_02215"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOB77289.1"
                     /translation="MDKKFISKLIKREGSDNILGRGVINLLLFNAKILPINVHKAIIG
                     LFMRLLVHRKHKWVLRTNLQILYDNTLSDKELEKKVNKILKLYKDMFIEMAHYTSRNK
                     MPDYDEFIEEVIGKEYLDEAYKQGKGIIALSAHLGGFITLTHALTLIGYPNCATIMRE
                     QDDKKIEEKFNKLRNNLGVKGIPQSVARSTGVALIRFLKQNGILIILSDQKFDDGVKV
                     KYMGRYKWTAKGPALLSLRLGSPVIPMYNIRCDNGKYKLIIKPPIELVKTGDTEYDIQ
                     VNTQMFVNELESMIRKYPDQWYAFNPWWQYTEEQLAEIEAEEAKETIETQIGDIIVEE
                     LSEEDLEEGLEINPL"
     gene            complement(21657..22721)
                     /gene="recF"
                     /locus_tag="PKI65_02220"
     CDS             complement(21657..22721)
                     /gene="recF"
                     /locus_tag="PKI65_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOB77290.1"
                     /translation="MILKRLELINFKIHSLLELDFTTGINYIVGDNGIGKTSILDSVY
                     YLGLTRNPYNISDNKFVQFDKDFFWIKGFFLSDDNSPENIEVFYHINNGKRVNRNEKT
                     YKNFSSHLGLIPILFICPADIYELVHIQENRRKFIDQILSQQNKNYLNALQQYQKFLL
                     QRNHLLKQQSDYGKNFTNMLDAINSNLIPLNAKIYKYRAKLVDEINNLICNIFYSISD
                     IDIPVSISYESNVNIENVNNLYNESIDNDLRLMHTTVGIHRDKFYFIFNGDDISLIAS
                     QGQQKLFIIALKLSMYQWLSSHYNEHAILLLDDIFDKLDEKRTDRLLEQLFQFNLSQI
                     FITDTDKNRVVDNVNKVIKL"
     gene            complement(22796..23680)
                     /gene="folD"
                     /locus_tag="PKI65_02225"
     CDS             complement(22796..23680)
                     /gene="folD"
                     /locus_tag="PKI65_02225"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOB77291.1"
                     /translation="MKILDGKITAEQIKKEIKVEVSQYIDDGNRPPHLTTVLVGNNPA
                     SKTYVRNKEKNSKLVGFTSSTYNLPEDVSEKKLLDVINFLNNDKDVDGFIVQLPLPTH
                     IDEQKIINSIDPDKDVDGFHPVNVGKMVLGLDTYLPATPSGIIELLKRNNIETTGKHA
                     VVIGRSNIVGTPLSILLSRKSKFGNCTVTICHSYTPNIKDFAVQADILVAAIGKPAFV
                     TADMVKDGAIVIDVGINSIPDETKKSGFRSVGDVDYDEVSKKASWITPVPGGVGPMTI
                     ASLLMNTLKAYKKRFNLF"
     gene            complement(23677..25017)
                     /gene="ffh"
                     /locus_tag="PKI65_02230"
     CDS             complement(23677..25017)
                     /gene="ffh"
                     /locus_tag="PKI65_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOB77292.1"
                     /translation="MFDNLSDRLEQAFKKLKGQGRISEINISETLKEIRRALVDADVN
                     YKIAKSFTENVKDKAIGQQVIKSVSPGQMMVKIVYDELVQLMGGKAVELNLKSKPAVI
                     LLAGLQGSGKTTFAAKLALYLKNNNNRNVMLVACDVYRPAAIEQLKTLGESVNVTVFA
                     NEDEKNPIKIALEAFNYAKNNNIDTLIVDTAGRLAIDEDMMKEIRMLHETLKPDETLF
                     VVDAMTGQDAVNTAKAFNDVLDYDGVVLTKLDGDTRGGAAISIKATVDKPIKFSSIGE
                     KMDDIDVFHPDRMASRILGMGDIVSFVERAQQQVDEEKAREVEKKIKKNKFDFNDFYD
                     QIQQVKKMGNLKDLISMIPGMGKAVRDIDIDDDAFKLIEAIIHSMTPYEREHPEVLNG
                     SRKLRIAKGSGTSVQDVNTFLKQFEEMRKMMKKALTTNPKQLKRIGQLNKNLFS"
     gene            complement(25507..26115)
                     /locus_tag="PKI65_02235"
     CDS             complement(25507..26115)
                     /locus_tag="PKI65_02235"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOB77293.1"
                     /translation="MKYSTFKRGFDFLFSLIFLIILLPFFLIISLMIITTDGFPIFYN
                     QKRVGKDQKIFKILKFRTMKKNAETQGKLTIGEKDPRITKIGYFLRKYKIDELPQLWN
                     ILKGDMSFIGPRPEVPEYVAMYNDQQKEVLSVRPGLSDYASLRYINENEILSHYPSPD
                     DAYINILLPQKIKLSKKYIAEMSLKTDFEITIKTIKKILNNV"
     gene            complement(26096..27307)
                     /locus_tag="PKI65_02240"
     CDS             complement(26096..27307)
                     /locus_tag="PKI65_02240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOB77294.1"
                     /translation="MIPFSPPRIDEKNIEAVIEVLKSGWITTGPKVQKFEKLLSEYLN
                     NGPVLCVNSATAGLELSLRWYGVGPGDEVIVPAITYAATANVVLHCGAKPIFVDISPE
                     DFNIDIKKIEPLINNKTKAIIPVDIAGLPCHYTEILDLIDSSKKLFVPSNEKQEKLGR
                     ILLLSDAAHSLGAIYKNKKTGNWGDIIVFSFHAVKNLTTGEGGAIVLNLPDHFEKDNT
                     YNELKINSLHGQTKDALSKNKIGNWEYDIVEAGYKYNMTDIQAAIGIVEILRYDNDML
                     KKRKKIFEKYSDAFADDKRFICPIYKDNQRTSSFHVYQLRINDCDEATRDHLIRAIAE
                     KGVATNVHFKPLPMMTLYKNIGYDISEYPEAYKQYAREISLPVYYDLTDDMIEQVIIT
                     VKQSVNEVFNI"
     gene            complement(27408..27998)
                     /locus_tag="PKI65_02245"
     CDS             complement(27408..27998)
                     /locus_tag="PKI65_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOB77295.1"
                     /translation="MEVKYSQGDTLVEEGEKINKFLYIKEGLIKVSQLTDKKENRIIS
                     IARPHDNITLLTFFSSEKYLYTITALEDTLACTFPYRLMLDVIKSEKEFSYGFLNMIS
                     YASSKVINNFIKLSSRNLRGRLAYIILNFSDNIYKTDSFDLPLSRKEIAELIGMTVEN
                     VIRILSEFKKDGIINIDGKSIDILDKKKLQMISEFG"
     gene            28398..29396
                     /locus_tag="PKI65_02250"
     CDS             28398..29396
                     /locus_tag="PKI65_02250"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77296.1"
                     /translation="MKKIVENTNKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEIVTNHRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNITYVN"
     gene            29807..30943
                     /gene="amrS"
                     /locus_tag="PKI65_02255"
     CDS             29807..30943
                     /gene="amrS"
                     /locus_tag="PKI65_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOB77297.1"
                     /translation="MDRRSFLKQGLLTGISGILLSQMPFLNRLESKNIIDKMINENEQ
                     LAKFWENINDSTVRCLLCPNQCVIQDGQLGICKDRKNINGKLYSLSYGRIVSLNIDPI
                     EKKPLYHFLPMSTVLSFGTAGCNFSCKNCQNWDIAQASPLEIRSYHYTPQQIVKTAVA
                     QKIPSIAFTYNEPTVFYEYMYETALLAKNYGIKTILVSNGYINPEPLAELIPYLDAAN
                     IDLKSFDDKIYIKLNGGRLQPVLNTIKTLKKSGIWLEITNLIVPTYTDDLNMIDNMAS
                     WLVDNGCAETPLHFSRFFPAYKLSNLPPTPINTIEEATKIAKKRGMKYVYPGNVLDEK
                     ANSTYCPQCDSLLIRRQGYHTSIVGIINDGKCSKCGYKITGVWK"
     gene            complement(31033..31275)
                     /locus_tag="PKI65_02260"
     CDS             complement(31033..31275)
                     /locus_tag="PKI65_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOB77298.1"
                     /translation="MEYEEIKKRVIHLLDQIRPYLQNDGGDMEFIELTTDYIVKLKLK
                     GACHACPMAMITLKEGIEETLKTYIPEIKEVVNVID"
     gene            complement(31287..32375)
                     /locus_tag="PKI65_02265"
     CDS             complement(31287..32375)
                     /locus_tag="PKI65_02265"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOB77299.1"
                     /translation="MKQKILEALRSVIDPDLQKDIVTLGMVQDLDVKEGKVSLSLILT
                     TPACPLKKYFETQIIEKIKSIIPDAEVNVNISSKTTTVRSESIMGNIKNIVAVVSGKG
                     GVGKSTIAVNLAVGLAQKGAKTAFMDADIHGPSAPLMFGLENIQPQIIDKDHKQIITP
                     LEKYGTKIMSMGFFIDPDKALIWRGPMIGSAFKQIMEDTDWGELDYLVIDTPPGTGDI
                     HLTIVQNYSLTGVVIVTTPQKIAINDAVKAINMFKDPNINVPVLGIVENMSYFIPPDA
                     PDKKYNIFSGSGSIEEISKMYNIQILGQIPIDPQVCDEADKGRPAILDQNTNYGRELE
                     KMVDNTVRAIAIRNASLPKTKIVEIKYH"
     gene            complement(32479..33114)
                     /locus_tag="PKI65_02270"
     CDS             complement(32479..33114)
                     /locus_tag="PKI65_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOB77300.1"
                     /translation="MKFEEYNLQKRISQGEGQHLDFKFAINDSKKIARSMSAFANADG
                     GILLIGVKDNGKIVGIKSEEEYYMIETAAMLYCKPSIKYDVIKHDIEDKEVLEVIVYY
                     SSDELIYAPDEHNNWCIYIRKNDKNYVASSIYEDVWLARHNNEIVKIDDIDNFRKNIT
                     KFSLKKIFTFKEFQKYFKLNYKDSYSLLMKLILLGWVDFFFDDEGEKYQFI"
     gene            complement(33205..34953)
                     /locus_tag="PKI65_02275"
     CDS             complement(33205..34953)
                     /locus_tag="PKI65_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOB77301.1"
                     /translation="MKKINQLFCILMIFIIKFSILNAQQIIISTEIIEENGKNFYIHT
                     VEAGQTVYSIAKAYNVSLDDVYKHNEWAKEKIKPGEKLKIPIIKTETTPKAVQYDTVF
                     HIVKPKETLYSISKEYNVDIEDLRDWNKEILASGVLKVNDKIIVGFTEKKLTIPDDIQ
                     QISKDVSTNIINYKVKEGETLYYIAKQFNTSIDSIVKWNKLPSHFVKSGQILTLKLPI
                     EDLNEKKCKKGHLPDKVKIIAFVPAIDEVTSEMEMSSTNIAINYLEGYEFLQFYEALL
                     LLQNLPIDYVTTFEIIPVAQVKNKKEFEKNLAKVNWQDVDIVLGPLDDQFLSYTLPFI
                     SDKFLWLHYLQKVDVDNAIPEVQFLSNITEEMKRIAEYITNNVKREAPIYIFHTADTI
                     EKYNARLLKQLLNKNGFNNVNFSNTISSLPSGLRDQTVVVALTLDEPTAGNIIRHLGT
                     SIKDLSLLEFFCPSSWLYFDNLDFYFFNKISPYLFFNNCYLDRKNKEIANFVNEFINS
                     YNTFPSIYAIYAYETMNFLNELIGEYGDNYKNCLTSYDYRGTICNCKFVKNGNAFANN
                     AVKIGKMKDYKIEIIY"
     gene            complement(35211..35696)
                     /gene="ispF"
                     /locus_tag="PKI65_02280"
     CDS             complement(35211..35696)
                     /gene="ispF"
                     /locus_tag="PKI65_02280"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOB77302.1"
                     /translation="MDKIYRIGIGYDIHRLVKNKKLILGGVEIYYKYGCRAHSDGDVL
                     IHAIIDAMLGAINLGDIGSHFPDNDPAYKDIASVELLKKTIEICEPYNYKISNIDSII
                     ILEKPKLAPYIEMIQKNLCNILNISKDKLSIKAKTNEKLDSIGRGKSIAAYCIVLLEK
                     I"
     gene            complement(35696..36901)
                     /gene="porV"
                     /locus_tag="PKI65_02285"
     CDS             complement(35696..36901)
                     /gene="porV"
                     /locus_tag="PKI65_02285"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOB77303.1"
                     /translation="MRKIEISFLTLICFFNISVLKSQSYPDILGQEGDRLNTITTAVP
                     FLLIAPDARAGSMGEVGAATTPDINSMHWNPAKYIFIDGDYGVTISYTPWMRKLIHDI
                     NLAYVSGYYKFDKQQAIAASLRYFTLGEIQFTGMTPDENLGTFSPTEFAIDVAYNRLF
                     GDNFSFAIALRYIYSNLSGGATVGTGHAETKPGQSVAGDIAIFYKNDFKINKQDARIA
                     FGMDISNIGAKITYSEIGYRAFLPTNLRIGPSFTYNIDDFNAITVAFDLNKLLVPTPP
                     YYAYDSINEEYVIAKGKDPHRSVVDAIFSSFWDAPDGFKEELHEVIFCGGVEYNYAEQ
                     FFVRAGYFNEHKTKGNRKFATFGVGVRYNIFDLSFSYLVPFEQHHPLENVLRFTMSFN
                     FFKPQNKPK"
     gene            complement(36963..40733)
                     /gene="porU"
                     /locus_tag="PKI65_02290"
     CDS             complement(36963..40733)
                     /gene="porU"
                     /locus_tag="PKI65_02290"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOB77304.1"
                     /translation="MHKLFCFIFTFLPIFIFSQEILNKPYVLEYFNGTELPYYIMPSK
                     KIPVNSKWDYYLNIDSTYFVSSNQLSKYSHNIASDFQIYYQEQIVSGEKQIIFFVCPF
                     RKVSDTLIEVLFTWEIKTIVSPYTEDLITQKRAYTNESVLSKGKWIKLSTTHKGFHCI
                     NYENLLSWGLISSSIPSDQLRVFCNGSRMLPEANNDFRYDDLHEISIEVYDGGDGMFG
                     PGDYAIFYVEAPIYWDFDHGNNRYVHSRNLYSEKNFVFVTISNEGVAQRINLANTPID
                     NEDINVTTFMDYALSEKELYNFNKSGKLWVGDKFDFTLMYNYNFSFPNIIADSNVYIR
                     TIVYGRSEFPSSFRLTYNNYNYNIPISTIHGYLDDYAKKGEANIIYKSSTPDINITLR
                     YQKGSNNSAVGWLDYIEVLAWRELKFYGNQMSFSHPFLPKNKIAKYQISNTPNTISVY
                     DISTPEHPSKISGLHNANVFQFTDSTNKLKEYIAINGNYYTPNYEGIVENQNLHGLSQ
                     VDYIIIAPDKFYPMAKKIGDLHKYEEELSYIIVSPDKIYNEFSSGKQDISAIRDFIKM
                     FYDRAINEDEKPKYVLLFGDASYDYLNRIKNNTNLIPTFQSLASVSPTASYASDDFFA
                     LLDDGEGYDCYGALDVAVGRFPVNTENEAWAMVEKIERYYKKQGANTGITICDDAECL
                     VPQLGDWRNRVVLIADDEDGNTYINQAENIANIIETKAPQLNIQKIYLDAYPQISTPA
                     GQRAPEVNSAINDAMNNGMLLFNYIGHGGEEGLAHERIVQISDILSWHNACAMPLVVT
                     ATCEFSRFDDPERTSAGEYVILNPKGGAVTMMTTSRIAYSSYNAVLNNALIDTLMDFV
                     NSDRPAFGDLFKIAKNSANNAVPLRNFVLLGDPAARITLPYNKIIIDTINNNSISAID
                     TLSAFEFVTIKGHIEDFQADVVNNFNGTIYVTMYDKKQTLSTLGTDISSFPTTFESFT
                     NIVYKGSATVKNGEFKIDFFMPIDIQYDFDKGRLTLYAENGMEDASGYTHDFLIGGTA
                     IPIADNEGPEINLFLNSINFRNGDIVDENPTILALISDEHGINLTGQAIGHDLVAWID
                     YDYKDPYILNPYFRYFLDSYKEGYLIFPLHDLSAGYHSLTLKAWDVFNNSSEATVTFQ
                     VIPKHNLTINTVIPYPNPMTTSTTFFIQHNQACCDNKIIIEIYDLLGKKIAKLYDESY
                     NESYANTEIYWDGKSIHGNTVTPGTYVYKAWLENDNGTSALSSGKILILPEK"
     gene            complement(41132..41314)
                     /locus_tag="PKI65_02295"
     CDS             complement(41132..41314)
                     /locus_tag="PKI65_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77305.1"
                     /translation="MRRFRGVATKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
BASE COUNT        14628 a         6274 c         5889 g        14710 t
ORIGIN      
        1 tatagattat ctaggaatct acgtttctgc tctttgggta gagcttgata atctagtaga
       61 ttaaaattat ccataattat ttttttgtaa aattaaataa aaattatttg atggtagtat
      121 aaaatacgta aagtaaaaca atgaatgata tcaaataagc atccatccct attcagtata
      181 cttttttttt attgtttatt gatttcttaa aaaaattata aatacttatt ataaaaataa
      241 atacttttgt ataaaaaatg tcttacataa gaataagtaa agaatttcat ttcgaaatgg
      301 ctcatgtatt agatgagtat gatggtttgt gtgcttatgt acatggacat tcatatacat
      361 taatagtaac tgtaaaaggt aagcctaata cagataaaaa ttctccaaaa tatggcatgg
      421 taatagattt taatacatta aaatctatta ttcaaaaaga aataattgag ttatttgatc
      481 attcattaat agttaataaa aatgctgaat ggttagataa agttgaaaat ataaaaagtt
      541 tttctagaat aataaaattg ccctttcaac ctaccagtga aaatctttta ttacatttta
      601 ttgacattat aactccacat ctacctccag aagtaacttt agtgcaggta catctcaggg
      661 agactaatag ctcatatgta gagtggcttt atgaagataa caagtaaata taaatctgta
      721 tgaaattttt taaaaaatta tacatttaac aataatttaa aaaattgtga aaaagttatt
      781 aaagcaattt ttttattttt taaagctcac aagcattaaa atatttgaat ggttttataa
      841 agtaaaccct aaaatattaa gattttgtaa tataatttta tcttttttat ctattttcgc
      901 tggattatta acgatctttt tattaggaaa tctttatact ccaactgtag ggcacaatct
      961 agaaccttat attgatttta ttagctggag tataagtata atatatctaa ttcaaattat
     1021 tttattgatt ttacctggtg gtaaaaaatt ttcatggagt aatttattat taaaaatgat
     1081 ttttatcatt actattaatt attttgtcta ttttattcct gattttatct ctccttattt
     1141 acatgatgtt ggatacatac attattataa attattttta atatttctag tatttataag
     1201 gactattagt ctcttggctc aaaatctata caaaatatct atacatcctg ccaaaatttt
     1261 tatcataagt tttattatta ttatcataat aggttcgcta ttattattat tacctgcagc
     1321 tacttatagg gatataaaat atgaagattc attatttgtc actgtatcgg cagtatgtgt
     1381 taccggcctt actactgtaa atatagctag tacttttact tggtttggca aattgataat
     1441 aataatatta atacaattag gtggactggg tataatgact ataactagtt ttttagcttt
     1501 atcattcaca cgaacagatt tatcaataaa ggaacaacaa ggtttggaat ttaatttagg
     1561 cagttcatct tttagggaaa ttaaaaaaaa tttgtatgct ataattttaa ttacagcatt
     1621 atttgaaagc ataggtgcta tactaatata tttttctgtg cccgatatct attttgataa
     1681 tctagctcaa cacatctttt tcgctatttt tcattccgta tcagcctttt gcaatgctgg
     1741 ttttagtaat ttacctgatg gtatgatgaa tgaagtattt gttaataatt acctaagcat
     1801 ttttacaata agcggtttaa ttatttttgg tggaatagga ttttttgtaa tcaatagctt
     1861 attaaaaaat attaatttta aattcaaaaa cttaattaga aaaatattta acaaacctaa
     1921 aataataaaa cctaaaataa taaaacctaa aataataaat atcaataatt taatagtcac
     1981 aagaaccaca ttaatattga ttattttagc aatagcagtt atattaatat tagaatcaaa
     2041 taatagttta atcagttata attggtttga taaagtagta caagcctttt tttgttctgt
     2101 aacaccaagg acagcaggct ttaatacttt agattttgct agtttcactc ctgcaactat
     2161 aatgtttata ataatattaa tgtggatagg tgcctcacct ggtagtactg gtggcggcat
     2221 caaaactaca tctatagcat tagccttttt aaatgctata tccattggaa gaaatcaaga
     2281 taaaataaca tttaaaaatc gtaccattag caatattaca gtgcaaaaat catttgtaat
     2341 aattgtattt tcacttttaa ctatatttat aggtattata tttgtttgtg cttttgatcc
     2401 tcatttaggc tttatgaata ttatgtttga ggtgttctct gcttttggta cagttggctt
     2461 gacattaggc atcactccta atctatcttt agcttccaaa atagtgataa tcatagaaat
     2521 gtttgtaggg cgtatgcaat ttgttattct gtttttagct attataaaaa gagccaaacg
     2581 tcgattaatc aaatatccag aaattgattt atatttataa aaaaaattaa atgagcaaaa
     2641 aagttttagt attaggatta ggtaatttcg gtagtgaatt agccaaaagg ctaactcatg
     2701 aagatataga agtaacagga atagatatag atcacaattt agtggatcta cataaagatt
     2761 ctattacaaa tgtagtagaa ggtgatgcta caaaagatca agtgataaag gaattagaag
     2821 tatctatgta tgatttttgc atagtttgta tcggtcacga cataggtgtc agcctcacta
     2881 tggtagctct gctaaaaaaa tatgaagcaa atattatata tgctagagcc attaatgcta
     2941 ttcacaaaca aatactatca ctattgaggg taaaatatat attcgaacct gaaatagaat
     3001 ctgcaggtag actagcaatg caaatagcta gtagaatggt gtttgataca tttcctatat
     3061 ctaaaaatta catcgcagta gagatagatg tacccaaatc ttatgaaaat ttgaaattag
     3121 aagatatcaa aatcaacgaa gaaattgatg tcttgatgtt aaaacgttat tcttatgtag
     3181 aaaatccatt cgagcgtagt gagctatatc atcctccagc ttatgaatat acacctcaca
     3241 aacatggcaa aacactaata catcaaaatg atcgtttagt gatttttggc ccagtcgaaa
     3301 aaataagaaa ttatattaaa atatatgata aagaaaatca agtatagtta atgtaaatat
     3361 taatatttta ccattttttg atcttaagtt ctaaatattt atatggttta ccaccaaatc
     3421 cttgataaaa aaaagccatt ttttcttcgt taccaccgtc aaaatctata attttgccat
     3481 ttgatttttt gaaattttct ataattttat atattaaata atgagtggcg ccagattttc
     3541 tacctttatc attagctgta aatgaatgaa atactaaaat gtcatgataa atagaaatca
     3601 gagcaatagc cataatagta tcttttattt ttaggcccca ccatcctcct agttgatatt
     3661 gttcgatatt ttttcctaat ttgataatta tatttcgcaa ttcagtttca tttagcatat
     3721 atggcgagtt gcataaaaaa tcatttaaaa aattagtagg tacatttgtt aattgtatca
     3781 aacatggttc gtttttttcc atttttttta tctttcttaa atgatgttca ctcatcatag
     3841 atctaagatc ttcgatgtct tctagtctaa gcaaataatt tattctacaa tcagatatat
     3901 atttactttt aaaacctaaa tcatcaatat aaaaatcagc aaatataact ttttttttaa
     3961 ttacacttat gagtgattga aaaatttctc cattaatatc aataagcgaa aaaggtctaa
     4021 aataagtaat catgggaggt tgcaaaaaat aaggaaaaaa caattttgtt tttttagcaa
     4081 atggaaaaat tatttgatag tcatcccata cgagcccata ccattgtggt gatactatat
     4141 ctagatacca gctaagtcca gtaatatgac gagatctaga caggcttact acctcattcc
     4201 acttattttc atttatatta tatctattta ttaattgcca actcatttaa tattttttaa
     4261 aaattcaata aaaaatggtg tccagccttg ccatctattt ttgtttgtaa agctttcatt
     4321 gtgaaacaat aaaactatct ctccccctaa tactaatgaa ctttttaatt gctcaaaagt
     4381 gtttttgaaa tcatcgacag tacttttttt gtagtctaca aatgtgccct ccatcaatga
     4441 ggtttggtgt aataataaat ttgttatttt ctcacctttc aaatcataat aatataatgg
     4501 atgtgatgtt cctagaggaa atccaggtaa agaagcataa ccaatagaat aatcttcttt
     4561 aatgcctata tttgctaaat tattcatatc tggcatatct aatctcaaat aatgagctct
     4621 accaattttg attttttcat tgatgatttt ttctaaagta gttttttcta attttattaa
     4681 atctggatta tcttttgcat aataactata gtgtatacct acatgatatt tatttgaaat
     4741 agttctaatt aaattcaaaa atcttcgaca atttatatga ttatttttat caaattttgt
     4801 tgttttattt gagagcaaaa agaaaaaaat caatggattt tgtaaatatt tacctatttc
     4861 taataataaa tcaaaatgat cgtaatcatc ttttttgatt tgcatcagtg tagctatcct
     4921 attaaaaaca tccaaaaatt ttaaatttaa taaatctcta aacaagcccc ctatattcct
     4981 atagaaacct ttgcatagat atgtgtatgc tatgtctaca tctatagtta cttgtaattt
     5041 ataaattttt tcaaattgaa taggtaaatc tttgaatctt gtttttaact ctttagctaa
     5101 taaaaatatc catttatcta cccaaggata aataaaagga aagcctctat gaaaaatagt
     5161 tgaattctcg ggaataaatc ttccatgaat atcaaaatat ttattaaaat acctttcgta
     5221 accagaaata caataaaaaa cagctgaaaa aatatcttga ttaaaataat tttcttgatt
     5281 aataggatac aatgataaat gattatcact tttttcagtt gttggtattt cttttgttaa
     5341 aaaattatca tttaataaag gtgaaatctt aaaaatattt atgctttcgg tagatggctt
     5401 ggtactatat ctaatatgag caaaagcatc taagtcattt attaaaatat agggaaactc
     5461 taataccgtc ttaaaaataa aatctaaagt atattttagt ctctcagaaa tgaaatctga
     5521 tgctatgata acaggagtca tttttatttt tttgttttga taccagaaac aactaattga
     5581 aaaataagag tccatccaaa gggaaagagt aattgaggta aaaaaggaac gaaatatttt
     5641 aattttaatc tagaaatgag ccaacaatat atgaacatat ctagatgtag cataagatac
     5701 gcaaacatac catgggatat tttttgttta aatttccaat gtgacggttg aataaaaggc
     5761 ataatggtac catagataaa taatgctatc aatactgccc accattcttt gcctgctatt
     5821 ccccaccatc tattaaataa aaaaaagact agtgttacga gtgagattct cgctccccat
     5881 cgataaactt taaagctttc ttctaaaaac tttgagaatt caggatcttg tgatttcata
     5941 attcacctct tcattattta ttttttgcat aatttaataa tttttcacaa ataaattttg
     6001 ctgtttgtcc atttccgaaa tattcgggaa aattagttgg agggtttgaa ataaacgaat
     6061 tagccagtga taaaaaatca tctatgtttt gtggttctaa agcacctgtt gcagcttcca
     6121 aaatttctac ccattcggtc tctctacgca taataatgca gggcttttga taaaaataag
     6181 cctctttttg cacacctcca gaatcagtta atatcaaaga agcaaaattc tccattttta
     6241 tcatatcaaa gaatgaaagt ggttctgtaa caatgatttt atctaataaa tatttcaatt
     6301 caaatgattt tattagcttt ttggttctcg gatgtaacgg aaacactaca gaaatattaa
     6361 gattttcaac aatagcattt agtttttcta atatttcttt taatctctta gggtcatcag
     6421 tattagtatt gcgatgtata gttgctatga caaatttttt atcttttaat ttaagttttt
     6481 ctatgatatt agatgatatc atagcttttt cataataata aatgctattg tcatacataa
     6541 tatcaccaga tagatatata gcgggattgt ctgcagaatg tggcggttca ttatctaaat
     6601 gaaacccttc atattgcaaa tttctaagtc ctgtaatggt aggagtaaaa agtagtgtag
     6661 aacagtgatc gcaaacgata cgattgactt cttcgggcat ttgtttatta aaagatcgta
     6721 aaccagcctc tatgtgagca attggaatat taatcttgat cgcagctaca gcaccagcaa
     6781 gtgtgctatt agtatcacca tataatacta caatatctgg tctatattta ataaaaacac
     6841 tttctaattt ttctagcatc ttaccagtca taactgcatg actggcagag ccaataccta
     6901 aattcaaatg aggctgagga atatccatct catcaaaaaa aatctcagac atatttgcat
     6961 catagtgctg tccagtgtgt attattatat cttcgatctc ttctttgtat aagtttctta
     7021 tagttctact aagtgctgcg gcttttatta tctgcggacg agcacctatg atattgcaaa
     7081 ttctcataaa taaaatttat tagcgaaatt acattttttt agtgatatag gatttagagt
     7141 aataatgcaa gtaaaatagt ttttattcag caattattaa aaaataattt ttcttgcctt
     7201 tctgaactat taaaaattta tttttaataa gattattagc atctattatt agattttcat
     7261 tatctactac ttctttattt aggtataatc ctttattagc tatcatgcgt ctaacttcac
     7321 tttttgaatt aaaaatctta gtacttactg ctagtaaatc cactatatta atccctgatg
     7381 cgatcgattc actagaaata ttgtatttag gaattccagc gaatatagag aatattgagt
     7441 catcattcaa atttttcaat atatcaatag aaccatctcc aaatagaaat tcactagtat
     7501 ttttagctat ttctaatgct tgcacaccgt gtatcataaa agtaacctct tccgcaagtt
     7561 ttttttgcaa aattctatta tgtggtgcca tgtgatgctc ggcaatgata gcttcaattg
     7621 tatttttatc aagttcagta aaaatcttaa tatatttttc agcatcctca tcagatgtat
     7681 ttatccagaa ttgataaaat tcataggggg atgttttatc aggatctagc catacatttc
     7741 ctttttctgt ttttccaaac ttattaccgt cagcttttgt aatgagaggg caagtgatag
     7801 cataagcctc agcccccgtt acttttctta ttaattctat accagtagtt atattacccc
     7861 attgatcgct accacccatt tgaagcttgc aattatagtt tttatataaa tgcaaaaaat
     7921 cattagcctg tactaattgg taagaaaatt cagcaaaaga aagcccctct cctacttcac
     7981 cgttaatacg tcttttcaca ttttcttttg ccatcatgta atttacagtg atatgtttgc
     8041 ctacatctct gatgaaatct aaaaaacgat aatctttcat ccaatcataa ttatttagta
     8101 ataaagctcc atttttttta tcattaaaat ccaatagttt tgttacttgt tttttgattc
     8161 cttcttcatt ttttctaata atttcctcag ccaataaatt tctttcttca cttttaccag
     8221 aaggatctcc gatcatacca gtagccccac ccaataacag aataggtcta tgacctgccc
     8281 tttgaaaatg acgaagaagc attatagaaa ctaaatgccc aatatgtaat gaatcagcag
     8341 taggatctat gcctacataa gcagtacatc ctgattgtga taaatattct ctgacatccg
     8401 gtatcatctc atataccatc cctcgccatt ctaactcagc tatgaaatcc atattaaaat
     8461 aatgtttaaa ttttcaaatt acaaaaatac aaattttaaa tgcaattttt ttatttattt
     8521 ttttttaaac ctacatctta aaataataat attatgatta aagggaaaat atattttatg
     8581 aatgacaaaa gcaatattga atatactaca aaaagtcatt tttatattta atcattattt
     8641 tttattaaga taatttaatg aatattatag ttaaattgtt tgttaatata gacaaacata
     8701 agtgcaatat taaagaactt agattttctg acttttcgtg ttagattctt ttttttttta
     8761 aaaagcttat taactaaaaa tctttttggt ctaaacatgc tcttttattt gtttaatatt
     8821 aaaaggtttt ttatttttgc ataaaattat ttttaatgaa taatattaaa ataaaaggta
     8881 ttatcaaaaa attaaaaaag ttaaaaatta gaaaaaaata tactcaaaat agtttatcta
     8941 gattaattaa taatcattgc tctgtcatag ctcaaataaa attagatgaa agaaacatct
     9001 atacaaaaga attaaaacaa ttagcttata tatctactat ttcagatgat aaacttaaat
     9061 tatttgatta tattgtggat aaaattttac tcagtgacaa agtaaataaa gccgaaaacc
     9121 aaattaaatt tatagaaaat agtcataaat ctaaaaaaga aaattatcaa tttaattata
     9181 aaacaataaa tctttataag gaggctaaat aatggatata tcaagagaag gtatttataa
     9241 tttgctattg gacctagtca aaattccaag tgtttcacca ggaaaaggcg aaacgctaat
     9301 agctgagttc atacatgata ctctacgtga tctaccttac tttcaaaaac atacagacaa
     9361 tctgtattac attcctatac ccaatgaccc atttagtagg aaaaacgtat ttgctatggt
     9421 agaagccgag cctaaaacta accgtactat tatcttaaca ggtcatatag atgtagtaga
     9481 caccaaggaa gcacgatctc tagaagaggt tgcatttgac cccgaagaat acacaaaaag
     9541 aatcaaagaa ctagatcttc ctgaagaagc agcaaaagat ttagaatcag gcaaatattt
     9601 atttggcaga ggcgtaatgg atatgaaggc tggtatcgct atacagatgg ccctccttgc
     9661 cgaatactca caaaatccta aaaatattcc tataaacata gcatttttag cggttgtaga
     9721 tgaggagaat aattcagcag gaatgcgcac agctataacc cacctggcta aactacaaaa
     9781 ccagggctta gaatttgttg cctgtattaa tagcgaagga gtaataccta agtatcctgg
     9841 cgacaccaat agatacatag atatcggaac tataggcaaa ataatgcctt ttttttattg
     9901 tgtaggacga gaatcccatg taggacaata ttatgccggg ctaaatgcaa accttctcac
     9961 atcggcagta aatatgatac tcgaaggcaa tcccgaatgg gccgaaaagt ggagaggcga
    10021 agtctatcca ccccctgcat ccctcaaaca aaaagattta agagaaattt actctgtaac
    10081 tcttcctgcc agagcgatgg tttattttaa tcaccttaca gtaaccagca caccggcaga
    10141 aatactcgaa aaaagcaaag aagtagctac tcaagccttc gaaaaagcta taaatcactt
    10201 ggagcaaagt gcaaatgaat tttctaaaaa agcaaacact tcagtaccta taccctggaa
    10261 aactaaaatc ataacatggc aagagttatt gatagaagta tctaaaactt ttgaagggga
    10321 gctggataat tatattaaag aatttataaa taatttacca gatgaattgg atgaaaggga
    10381 taaatctctt gccactgtag gggagctgat tcgcctttat ccagataaaa acccaatgat
    10441 tgttgtggga tttttacccc cttattatcc tcataaaacc aacaaacgag aaatagaatc
    10501 tgagaaaaag atgcttgcag ctgtagaaga aataattaaa gaagctaagg atgaatttgg
    10561 cgaaatattg gccatttcag aacatttcgc atcaatcagt gacttgagct atttaggatt
    10621 tcaaggagaa aaagatgagc tcgtaccttt agctttaaac acaccaggat ggggaatcat
    10681 atatgatatc cctttagacg atctcttgtt gttagacatt ccagtcgtaa atctgggacc
    10741 tatgggtaaa gatgctcata aatttgttga acgcctagaa ctagactact cattagatgt
    10801 agtacccaaa ctgctcaaat cattgataca aaaattatcc taagcaaaaa aatagaaggg
    10861 ctcttgccaa aagaaatcga aaatatcaaa atacttgatc cagcatgcag gtctaatagt
    10921 tttttggtgg gagcatgcag ccatttgctc gattatcatc aacggtggta tgcagagcat
    10981 caaagtaata acaattacaa aaatgatgga aaatttcaat tacagagaaa tattaataga
    11041 gggagcgaga gctaataatc taaaaaatat cactctcagc atacccaaac gaaaaatcac
    11101 agttgtaact ggcttatcag gttctggcaa aacttcactt gcttttgata ctatctatgc
    11161 cgaggggcag agaaaatatg tcgagagtct cagtgcctat gctagacaat ttttaggtaa
    11221 aataatgaaa ccagatgtag ataagatcac tgggctctca ccagctatag ctatcaggca
    11281 attctctaca aatgtaaatc cacgatctac tgtgggcact atcacagaaa tatatgatta
    11341 tctcaaaata ttatatacta gaataggcaa aacccatagt cctatatctg gcagagaggt
    11401 gaaacattac aaagtagaag atgtagtgga atatgtgatg tcttttccag ataaaagtag
    11461 attttttata gtagctccat atattgaaat ggtcggtcga gatgtcgaag aacagctagg
    11521 tctctggcat ataaatggat acagtcgtgt attattggat aataatattt accttatcag
    11581 agatctgcta aatgacaata ataaattaac taagataagc tcggataaac tgtttttgct
    11641 agtagataga cttactaaat ctgatgatga agattttgct ttcaggatca aagatagcat
    11701 acaatcagca tttttcgaag gtgatggcaa aatattcatt gtgattctga gtggtaaaga
    11761 gagaaaatgg gtagaatttt ctaataaatt cgaactagat ggcatgaggt tctccatccc
    11821 agatgttaat tttttcagtt atacttctcc gtatggagca tgtccagtat gtgaaggcta
    11881 tggcactgta gtagatatag cagaacatct agtaatacca gatcccactt tatcgattta
    11941 tgaaggggca gtggctcctt ggcgtggcga aaagatgagt gaatggcaga aatattttat
    12001 cgcatattct aaaaaatata atttccctat tcataaacca tataaagacc ttacagaaga
    12061 ggaaaaaaat cttttgtgga atggacgcga tgatctcgaa ggtataaata gtttttttaa
    12121 gatgctacag gagaatactt ataaaataca atatcgcgtt atgttatctc gatacagaga
    12181 aaagactatt tgcaaggctt gcaaaggtac aaggttgaga aaagaaactc agtatgtaaa
    12241 gattcatgga tattcactgc cagatttatt gatgatgcag atagatcaag tgcgagacat
    12301 tataaatact tgggtaatta ctgaaaaaga aagagaaaaa gtaaaatacg ttatcaagga
    12361 gctaaatagt agaatagatt atttgataca agtaggcttg ccttatgtaa cgctcaatag
    12421 aggtatgaat acgctctctg gcggggaaat ccaaagagta tatttgagtc gagctctagg
    12481 ctccactatg acagatacat tatatgtatt agatgagcct acagtaggac ttcacccaag
    12541 agatacacac aaattaatcg atatattaaa aaaacttagt aaggcaggaa atactattat
    12601 agtagtagaa cacgataaag acatcatcaa ggcgtcagat tggatagtag atttaggtcc
    12661 taaagctggt agcgaaggtg gtaatgtggt tttttctggt ccaatagatc aattgtctgg
    12721 tgatcctaat agttttactg ctaaatattt gtatgatcca ttaccagcaa ttgattcaaa
    12781 aacaaaattt tcatttttag aaactaataa tagatacata gaattaaaag gagctaacaa
    12841 aaacaattta aaaaatataa acattagatt tcccctaaat cagttgatag ctatagtagg
    12901 tgtaagtggc tcgggtaaat cgaccttagt agtagatgag ctttatgaag aatttaataa
    12961 atatcttcaa catttcgatt ttgagaaaac caaacttagt ggcgacttta aattaattaa
    13021 agatatagtc ttaatagatc aatcttctat ttcgcgttct actcgatcca atcctattac
    13081 atttttaaag atttttgatt atattagaga actatacagt ctaatgcctt tatctattca
    13141 gcgtggctac aagcctagta ctttctcctt taatactacg ggaggacgtt gcgaaacttg
    13201 taaaggcgat ggatatatca ttattgaaat gcagtttatg gctgatgtaa agataacttg
    13261 cgaagattgt catggtaaaa gatttaaaga agatgttcta gaggttacat ttggacaaaa
    13321 aaatatttat gatattctac aaatgaccat cgatgatgct tatgagtttt tcaaatctca
    13381 atatagtttt tctaaaaata aaaaaatagc taatttgatc actcagactt tgaatggatt
    13441 agaattatta aaaaaagtag gcttaggata tctgaaaatg ggacaatcta cagatacatt
    13501 atcaggtggg gaggcgcaga gattaaagtt agctagctac ataggagctg gcgaaaagac
    13561 cgaaaaaaca cttttcttat ttgatgaacc taccactggc ttgcattatt atgatgtaga
    13621 tatgctttat aaaagtttca aagaattgat tatgaaagga catactattt gtttcataga
    13681 gcataatatt gaattaataa aacgagcgga ttgggtcata gagcttggac cagatggtgg
    13741 tgagcaaggt ggatatttaa tattcgaagg aacaattgag gattttataa aaacagatac
    13801 catcactaca aaatacttga aggattgaaa aatttattat agattattaa taatctataa
    13861 taaaacacct taaaaaatat ttataaatat ttttaaggtg ttttatctca tcatcaaaaa
    13921 tatcagcaat ttgctaaata taaaaatcta aaatctccaa cctaatcgta tcatagtaga
    13981 agccatattt ccgaaatatg attcagaaaa ggttgaaaag atgtcgacag tagtagtagt
    14041 ttgtcctacg gtctgtgacc attcaccatt tttatcttta taggacatca ttccgatacc
    14101 aatttctgca cctacaaaaa tattttcagc gaaataataa tctacgcctg tgaatatcat
    14161 aaatccccac ccgccatatt tatatttata atggccattt ttactatcat cataacctgt
    14221 tagattttga tctgtatttt tccatgtttc agtatatgat ccagtcaaaa aagcaaaatc
    14281 taaaccagca tatggagata gtttttcttt tatagaaaaa tgataagctg ctcctaagct
    14341 aatttgagta ctgaaatctt tctcttcata ggttccaagt ttagattcca ctggtaaccc
    14401 ttgatcattt aatccataaa tcttgtcaat acttttggtg gaatttatag ccaagcctaa
    14461 acgagtagat attttgtcat tgacaaaata tctcaatttt aaacttggtg cagcaaaagt
    14521 ggatcctgtc tgtatattta tagtcagtcc accttcgata gagaatggat ttgtgcccgt
    14581 aggcatctgt gcaaaagtaa caatgctaat agttactaaa attacattta ataaaatttt
    14641 ctttttcata atatattgtt ttagggggtt ggttttaatt gtttgaaaaa attttaatta
    14701 ataaaagtga tttaattaat aataatgatg attaattagt taatattatt ctttttaata
    14761 aatactaata atgtgtttaa ttgataaaat aaaattatta tttattatat ttttttataa
    14821 aattatatat atattttcat ataaatcaaa ttttttaaaa tctaattgat ggttctgcat
    14881 attttttagc tttacaacca aaaacaagat taattcctaa atgcacatta aggtatttta
    14941 gattatgagg cacgttaaca tatttaaatt tattatcacc ttcatcataa tattgatatt
    15001 catcatatat gagtggacct agaatattat ctgtagtcaa ataaaattga aatggcgata
    15061 gtgtaagtgc aaaaccgaaa cccagattag tatagtttct attagcaata gtataattaa
    15121 tagaagcttg aaaaatatga ctaaatttat gtaaagcttt aaaatgaaaa gctggataca
    15181 ctgatttgtt taaaaacatc aaatgcactc ctacccccat agtagtttta ggtgttatat
    15241 cataactcgc agtgagatat atcttagtag gcaaccaagt agtgtacgag ttgtatgtag
    15301 aatctatatc aaacacatct ttaatagaat ctaataaatc atctaatctc tcgtcaaata
    15361 atgagtctga accagcgatg agtggggcta catctactcc agtaaatgta aaactagcat
    15421 tttcgttacg atttataaaa tttaatggat catttttcca atttataaaa ccaagatcta
    15481 taatagatag gcctattatt aaattatcaa taggtttaaa agtaaagcct agatctagtg
    15541 ctccacctaa attatttttt aacaatataa aatctttaac aaaatccgta ttatcaacta
    15601 aactgtcaaa attatttata taattagtat cacgcatttc ttcgggcata ctagtgttta
    15661 gctctaacgt gccaatagca gtgatatcat acatttctcc agtagtgatg agtttgatgt
    15721 cagtattacc actatttata tttatcttac cagataatag ttttagacga gctcctacag
    15781 atattttgtc atttatattc atagcatagc ctaaggcata ttctctataa taactaaaat
    15841 ctaaagctgg atttaaagtt atagatttat ctaataatgc tccattacct ttatatgcaa
    15901 actctacaag atttttagaa tatgataccc aagtatttat tttttccata atattaaaag
    15961 aaaaataatg tttatttttt attttaaagc taaaagataa taattctata ttattatttt
    16021 gaaataaata attttttttt gcaagcttat ttactactga atttacattg ataaaagtag
    16081 tatcattacg tgtttctaaa acctggtatg gattgaatcc tgtatgagct atataaatac
    16141 tattagatga tataagaggc aatccaatat aaactctaca agtaggcatc aaagcaggat
    16201 ttaaatatgt ggactggggt attacaggta tatcattagt tgtgaaatta tattgaccgt
    16261 aaatattaat agacaatgca gttaccaata taaataaata tttttttttc ataataatta
    16321 ttttttttta attaaatgga taaggattaa ttagctgtgc ttgcgcacta accctcaaat
    16381 taatatattg ataagaataa atttttacaa tattattacc atggtctgta gtatttaact
    16441 tagctttaat aacagcattt cttacattaa ctaatgcatc ttttctagct ccttcaaatt
    16501 tattagtagc taatttagta ctcgggctaa taacaaaata atcaggtgct ggccctacaa
    16561 tgcccgaagc ggctattcgc tgatttggtg gtaagattga gtctataatg ttattttgtg
    16621 catctaataa ataaacttgt atatctgcat ctacaggata ataattagta gtgaaaattt
    16681 taaacataaa ccaatctaca tgtttgaaat atgtactatc accaaggtca agttctaatg
    16741 tatctatcaa aacaaaatct ctagctgttc catgaaatgg taattctact ctgccattta
    16801 aatgaagtct acttgtatct attacaaaat tactatgacc atttgaattt attcgtgcat
    16861 ctgctactac atctatgtaa ttaggactaa tattgaaggc tgatacaatg ttagaattat
    16921 tttttgtaat ttgataaaat gataacttat tttggccaat ctctgacaaa gaaggataat
    16981 ttagactcca tataggaggc atacctgtaa tggtcattga cgagctaggt agattttctc
    17041 tacgagcttt gatctgatta atattaatat ttataggaac acctatacta ttagtagtgg
    17101 ttaaaaatat tttaggatct tcccaattaa ttattccatc aaagtgatta ttatacagtt
    17161 tgatttttat agtatcacta tctaaattta aattataatc acctaaatac ccgaaaagtt
    17221 tagaaaaacc aatattattc aaaccaatac taagaataaa ataatatggt gatagattag
    17281 cagagccatc gccgtgaata ttaattttaa tgtcaaaaga taactggtta ttagctccta
    17341 aacgcaattt aatattgttg taatgtttgg aataatcata actcagtgaa ggaactgaaa
    17401 aattttcatt atgtggacta ctattcactg tagcattatt aatagagaaa tcaacatcta
    17461 ttggataatt aatattatga ttgatattta atgataaatc taatgatttc aaaatcatcg
    17521 tatctaatag catattatta ggtagagata gagtaaaaga gtaattgtga gtaacctcat
    17581 aatcactgct tagattgcca ccaggtatat taatggttag ctgactaaat gactgatttt
    17641 gacttggaat attaatataa ttttccatag tattagtagc gatattccct tgataaagta
    17701 aatagataag attagtatta tactcttcga aaacctctga gctatcatag tcattcatta
    17761 aatcccacat atatagatta gaatttataa taggtgctgc tacattagga ttccaatcag
    17821 aagtagcaat attatcaaca ttgaattcat cttttacaca actagtaaca aaaaaaataa
    17881 taaatcccat taaagataca aataaaaaaa agtttttcat ctttataatt ttttattttg
    17941 acaaaaatat ataataaaag tttaattgaa aaatattatt ttaatgaaaa tattattatt
    18001 ttcgtaaaaa atatatgagt ttaaaaaaat ggcttattgc agttcgtcca tggtcattag
    18061 cagcatctac tgtcccagca ttaatgacaa ttacttatgt ttatttttta aaaatagaat
    18121 attcactaga aataaatata ttttatggat ttttagcttt aataggagct ggaatatttc
    18181 aagccagtag caatattata aatgactata tagattataa acacaaagta gatagactag
    18241 aatctttagg tggaggacgt acaataatag aaaatataat gagaccaaaa tcattcataa
    18301 tttatggata tattttatta attatcggta taattcttgg attatttcta acatttcaca
    18361 caggttatca tttattatgg ataggtgcta ttggtatttt ttttactatt tattacacaa
    18421 aattcaaata tatagggctc ggagatttag taatttttat tatttatgga ccaatgattg
    18481 gccttggtac tgcttatgtt atgactaata atttattatg ggaagtgatg ctcatcaatg
    18541 tgccaattgc tttattaata gtagatattt tgaatgcaaa caatattaga gatattatat
    18601 ttgataaaaa agcaaaaatt aaaacccccg cgatgctatt aggactagag aaagctaaag
    18661 tttattatat agtactgatg attgctgcat accttttcac agcattattg atatttttaa
    18721 atttactaca tccattatgc ttcttagttt tattgtcttt gcctatagct ataaaaaact
    18781 ttaatattat aaatcatgct acaatagaaa ccctagaaga aataaagcac ttagatgcta
    18841 aaacttcgga attagtttta gtatatggaa tattattttc tatttctaat tttatagccg
    18901 gtttttttat aatataaaat tttttttaaa ataatttttt attatttttg catttattaa
    18961 taaaaaaata tatggaatct agacctgatg taagagaatt aataataaga gctgctgaaa
    19021 cattcggtaa gcaaactgca tttatttata atgataaaga gtacaatttt ttggatttaa
    19081 aagaaaatag ctttagactt gccaatagtt ttttggacct tggatataaa aaaggtgata
    19141 aaatagctat ttatttgccc aattgcattg aatatattta tgcatattat gctatttatt
    19201 ctataggttg tgtagtagta ccaattgatt ttttcctaag tcaaaatgaa gtaatagcta
    19261 tagctactca ttgtgaacta aaaggaataa ttactactac agaagcaaaa tttaacttat
    19321 tagaattacg tcaatctata ccttctctga aagatattat tactatcgaa gataattcga
    19381 agtttttatc attttggaga ttagctacca attctaagcc agatttggat gatcaaggta
    19441 taactacaaa tatgatatcg agcatatttt acacctctgg cactactggt atgccaaaag
    19501 gtgcagcttg gaattatcta catattcatc taggagctga gcaatataaa tattttggca
    19561 cttatagcgg cttagaagat cgaatgagag tagatgagag agagcgatta ttagctccaa
    19621 tacctttttc gcattctggc ggagtattat atcccatgat ggctataaaa tttggtgtta
    19681 gtacagtaat tatgccacag tttaatcctt atgaatttgt aagattagta gatcgttggc
    19741 aggtaacggg ctttcacatg gtgccaccta tgtattacgc tattctatac ttaaaagata
    19801 taaagaaata taaattagaa tccttactgt gggcagctgt tttcggagca ccatcatctg
    19861 ctgaattaat gatgagattt gaaagattat gtcctaatgc tgttatagta aatggctggg
    19921 gtatgacaga ggtgatacca cctacgagtt tatctactcc aggtaacata cgttctatcg
    19981 gcaagcccac tcctaatgtg gaattaaaga tatttgacaa ccatggaaat gaagttaaaa
    20041 ttggtgaagt tggggaatta gttgttaaag gcaaatcagt atttttaggt tattataaag
    20101 agcctgaatt gaacaatgaa gttttcaagg atggatggtt ctatactggt gacttagcta
    20161 gaaaagatga agaaggtaat tattatatca ttggcaaatc taaagatact atcaaagtag
    20221 gtggtgagct tgtatgggct gctgagatag aggaattact actaaaacat ccttgtgtga
    20281 aagaagccgc tgccattggc attccagacc atttacgtgg tgaaatagtg aaatgctata
    20341 ttgcattacg cgaaggagta gttttcccaa aacaatattt attagaatat atgagggaaa
    20401 atttggctaa atttaaggtt cctagagaga ttgaatattt agatgaatta cctaaaacag
    20461 gctcaggcaa agtaaataaa gctgcattaa aaaatttaaa caaaaattaa aatatggata
    20521 aaaaatttat ttctaaatta attaaaagag agggatcaga taatatttta ggtcgaggtg
    20581 tgattaattt actactcttt aacgctaaaa tattacctat taatgttcac aaagctatta
    20641 ttggcttatt tatgcggtta ctagttcaca gaaaacataa atgggttttg cgtactaatc
    20701 ttcaaatact atacgataat acattgagcg ataaagaact agaaaaaaaa gttaataaaa
    20761 ttcttaaatt gtataaagac atgttcattg aaatggcaca ttatacctct agaaataaaa
    20821 tgccagacta tgatgaattt atcgaagaag tgataggtaa agaatattta gatgaagcat
    20881 acaagcaagg caaaggtatt attgctctct ctgctcattt gggtggtttt attactctaa
    20941 ctcatgctct cactttgata ggatatccta attgtgctac tattatgcgt gaacaagatg
    21001 ataaaaaaat tgaagaaaaa tttaataagc ttcgaaataa cctaggtgtc aaaggaatac
    21061 ctcaaagtgt agctcgcagt accggtgtag ctcttattag atttttaaaa caaaacggaa
    21121 tacttattat tttatcagat caaaaatttg atgatggggt gaaagtgaaa tatatgggaa
    21181 gatataaatg gacagctaaa ggtcctgccc ttctttcttt gagattagga tctccggtaa
    21241 tacctatgta taacatcaga tgcgataatg gtaaatacaa gctaattatt aagccaccta
    21301 ttgagctagt taaaacaggt gatacagaat atgatattca agtaaatact caaatgtttg
    21361 tcaatgaact cgaaagtatg attagaaaat atcccgatca atggtatgct ttcaatcctt
    21421 ggtggcaata taccgaagag caattagcag aaatagaagc tgaagaagct aaggaaacta
    21481 tcgaaactca aattggtgat atcattgttg aagaactttc agaagaagat ttagaagaag
    21541 gtttagaaat aaatccttta taataattat atataataaa tctcttcgat atacagatta
    21601 gatcaaacac aaaagtgtat atttatagaa gaaaaaaatt gatattaaag taaaatttat
    21661 aattttataa ctttattaac gttgtctact accctatttt tatctgtatc agtaataaaa
    21721 atttgactta aattaaattg aaaaagttgt tctaaaagcc tgtctgtcct tttctcatct
    21781 agtttatcaa aaatatcatc taataataaa atagcatgct cattataatg actactcaac
    21841 cactgataca tagatagttt aagagctata ataaacaatt tttgttgccc ctgcgatgct
    21901 attagcgaaa tatcatcacc attaaaaata aaataaaatt tatctctatg tatacccact
    21961 gtagtatgca ttaaacgtaa atcattgtct atgctttcat tatataaatt attaacattt
    22021 tctatattta catttgactc ataactaata gacacaggaa tatcaatatc agaaatagaa
    22081 taaaaaatat tacaaatcaa attgtttatt tcatccacaa gctttgctct atatttgtaa
    22141 attttagcat ttagaggtat tagattgcta ttaattgcat ctagcatatt agtgaaattt
    22201 ttgccataat ctgattgctg ttttaacaaa tgatttcttt gtaataaaaa tttttgatat
    22261 tgctgtaatg cgttcaaata atttttattt tgttgagata aaatttgatc tataaacttt
    22321 cttctattct cttgaatatg aactagttca taaatgtctg caggacaaat aaaaagtata
    22381 gggatgagac ctaaatgaga tgaaaaattt ttataagttt tttcgtttct attaactctt
    22441 ttaccattat taatatgata aaatacttca atattttctg ggctattatc atcagagaga
    22501 aaaaaacctt taatccaaaa aaaatcttta tcaaattgaa caaatttatt atcagaaata
    22561 ttatatggat tacgagtgag tcccagataa taaacactgt ctaaaataga agttttgcca
    22621 ataccgttat ctccgacaat ataattgata ccagtagtaa aatctaattc taataagcta
    22681 tgaattttga aatttataag ttctaatctt tttaaaatca tgataaaaaa ttacagtagt
    22741 tttgatcatt acctcctagt acagaaaaag tctattattt ttattcacaa aaagattaaa
    22801 ataaattgaa tcttttttta tatgctttca aagtattcat aagcaaagaa gctatagtca
    22861 taggtcctac cccccctggg acaggagtaa tccatgaagc ttttttagat acttcatcat
    22921 agtctacatc acccacagac ctaaaaccac tttttttggt ttcatcagga atggaattaa
    22981 tgcctacatc tattactata gcaccatctt ttaccatatc tgcagttaca aaagcgggct
    23041 taccaatagc tgctactaaa atatcagctt gcacagcaaa atctttgata ttaggagtat
    23101 aagaatgaca aatagtaact gtacaattgc caaatttact ttttcgagat aataaaatcg
    23161 ataacggagt acctacaata ttagaacgac ctatcaccac tgcatgtttg cccgtagtct
    23221 caatattatt acgtttgagt agctcgataa ttccactagg agtagcaggg aggtaagtat
    23281 ctaatcctaa taccatttta cctacattga ctggatgaaa accatctaca tctttgtcag
    23341 ggtctatact atttataatt ttttgctcat ctatatgagt aggtagaggt agttgcacaa
    23401 taaagccatc tacatctttg tcattattca aaaaattaat gacatctaat aatttttttt
    23461 ctgatacatc ttcgggtaga ttgtaagtag atgaagtaaa gccaacaagt ttactatttt
    23521 tttctttatt gcgtacataa gttttactag ccggattatt gcctacaagt accgtagtta
    23581 gatgaggtgg tctattgcca tcatcgatgt attgagatac ttctactttt atctcttttt
    23641 tgatttgttc agctgtaatt ttaccatcta aaattttcat gagaataaat ttttatttaa
    23701 ttgtcctatt ctttttaatt gtttaggatt tgtggtaaga gcttttttca tcatttttct
    23761 catttcttcg aattgtttca aaaaagtatt tacatcttgt acagaggtac cactaccctt
    23821 agcaattctc aattttctag atccattcaa aacttctgga tgttcacgtt cataaggcgt
    23881 catagaatgt ataatagcct ctatcagctt aaatgcatca tcatctatgt caatatctct
    23941 aacagctttg cccataccag ggatcatact aattagatct ttaagattac ccattttttt
    24001 tacttgttga atctgatcat agaaatcatt gaaatcaaat ttatttttct taattttttt
    24061 ctctacctca cgagcttttt cttcatctac ttgctgctgc gcacgctcta cgaagctaac
    24121 aatatcgccc attcctaata ttctactagc cattctatca ggatgaaaga catctatatc
    24181 atccattttt tcaccaatac tagaaaattt aataggttta tctacagtag ctttaataga
    24241 aatagcagct ccaccacgtg tatcaccatc cagtttagta agtactacgc catcataatc
    24301 caaaacatca ttaaatgcct tagctgtatt tacagcatct tgaccagtca tagcatctac
    24361 tacaaaaagc gtttcatcag gtttcaatgt ttcatggagc attcttatct ccttcatcat
    24421 atcctcatcg atagctaaac gtcctgctgt atctactatg agagtatcga tattattatt
    24481 tttagcataa ttaaatgctt ctagagcaat ttttatagga tttttttcat cttcattagc
    24541 aaatacagta acattaacac tctcccccaa agtttttaat tgctcaatag ctgctggacg
    24601 ataaacatca caagcaacca acattacatt tctattattg ttgtttttaa gatatagagc
    24661 tagtttagcc gcaaatgtag ttttaccact accttgcaat ccagctaaaa gtattacagc
    24721 cggtttgctt tttagattaa gctccactgc tttaccaccc attaattgca cgagctcatc
    24781 gtacactatt tttaccatca tctgccccgg agatacgctt ttgatcacct gctgccctat
    24841 agctttatct ttgacatttt cagtaaagga ttttgcaatt ttatagttga catcagcatc
    24901 tactaatgct cgtctgatct cttttaatgt ttcggaaata ttaatttcag atatgcgtcc
    24961 ttgtcctttt aattttttaa atgcttgctc gagtctatca ctaagattat caaacataaa
    25021 taatttttta caaaattaca aaataatgtt gaataatgaa agtggaagta tttgatttat
    25081 ttgtttggcg gcatttattt taaaacggcc aataattttt ggtaataata tttttacgtc
    25141 gttgaatcca ttgtgggaat tcattttgtt gttgggtaat tcgtttttgg ggtaattcgt
    25201 tgtagggaca attcgtgaat tgaccctaca aggtgttttg tctggtggat catctgattt
    25261 catggctaaa gcccatttcc ttttctttgc tgtcccacga cctaaaggtc gtggttaact
    25321 ttgttcctcg accttttctt tgatttgtca taaaactttt tattaatcat aaaaaccttt
    25381 ataatcatcc taaacttcta aactactaaa ctcctccact actaaacttt atccatcaat
    25441 ctaatcatcc gaaaatcttc ggttctgact atatgtgttt gggggtcgaa tggcaataca
    25501 ccctatctat acattattta atattttctt tattgtttta atagttatct caaaatcagt
    25561 ttttagtgac atctctgcaa tatatttttt acttagcttt atcttttgtg gcagcaaaat
    25621 atttatgtaa gcatcatcag gcgaagggta gtgagataat atttcatttt catttatata
    25681 gcgaagagag gcataatctg atagaccagg tctgacactt agtacttctt tctgctgatc
    25741 attatacata gctacatatt ctggcacttc tggtctggga cctatgaaac tcatatctcc
    25801 tttaagaata ttccacaatt gaggtaactc atctatttta tattttctta aaaaatagcc
    25861 tatcttagta atacgaggat ctttttcacc aatagttaat ttgccttgag tctcagcatt
    25921 ctttttcata gttctgaatt tcaatatttt aaatatcttc tgatctttac ctactctttt
    25981 ttgattataa aatataggaa aaccatcagt agtgattatc attaggctga taatcaaaaa
    26041 aaatggtaaa agaattatca aaaatattaa tgaaaataaa aagtcaaatc ctcttttaaa
    26101 tgttgaatac ttcatttaca gactgtttaa ctgtaattat tacttgttct atcatatcgt
    26161 cagtaagatc ataatataca ggcagtgata tctcacgtgc atattgctta taagcttcgg
    26221 gatactcaga tatgtcataa ccaatatttt tatataatgt catcatagga agtggtttga
    26281 aatgtacatt agtagctaca cctttctcag ctatagctct tatcaaatga tcacgagtag
    26341 cctcatcgca atcatttatt cttaattgat aaacatgaaa agaagaagtc ctttgattat
    26401 ctttataaat aggacaaata aatcgtttat catcagcaaa agcatcacta tatttttcga
    26461 aaattttctt cctttttttc aacatatcat tatcatatct caaaatttct actatgccaa
    26521 tagcagcttg tatatctgtc atgttatatt tatatccagc ttctacaata tcatattccc
    26581 aattaccgat tttattttta gataatgcat ctttagtctg tccatgcaat gaattgattt
    26641 tcaattcatt atatgtgtta tctttttcaa aatgatctgg taaatttagc acaatagcac
    26701 ctccttcgcc agttgtcaga tttttgacag catgaaagct aaatactatt atatcgcccc
    26761 aattgccagt ttttttattt ttataaattg ctcctaaact atgagctgca tcagatagta
    26821 gtagaatgcg tcccaatttt tcctgctttt cattagatgg tacaaataat tttttggatg
    26881 aatcaatcaa atctaatatt tcggtataat gacatggtaa gccagcaata tccactggaa
    26941 ttatagcttt ggttttatta tttattagag gttctatttt cttaatatca atattaaaat
    27001 cctcaggtga tatatctaca aagatcggtt tagcaccaca atgtagtact acattggcag
    27061 tagcagcata agtaatagcc ggcactatta cttcgtctcc aggtcctact ccataccatc
    27121 tcaaggataa ctcaagacct gcagtagcag aatttacaca cagcactggt ccattgttta
    27181 aatattctga taataatttt tcgaattttt gtactttggg gcctgtagtt atccaacccg
    27241 attttaatac ttctattacc gcttctatat ttttttcatc tattctaggt ggtgaaaaag
    27301 gaatcatttt ttttacaaaa ttaaataatt tattagtttt aataatttaa taaactaact
    27361 gcaacaagat aatatctttg ttttttttga taaatctaaa aataatttta tccaaactcc
    27421 gaaatcattt gtagcttttt tttatctaaa atatctatgc ttttgccatc aatattgata
    27481 atgccatctt ttttaaattc acttaaaatt cttattacat tctctacagt cataccaatt
    27541 aattcagcta tttcttttct agataaaggc aaatcgaaag aatcagtttt ataaatatta
    27601 tcagaaaaat ttaaaattat gtaagctaaa cgacctctca aatttctact actaagtttt
    27661 ataaaattat taattacttt actagaagca taactaatca tgttcaaaaa accataagaa
    27721 aattcttttt ctgattttat cacatctaac ataagtcgat aaggaaatgt acaagctagc
    27781 gtatcctcta aggctgtaat agtataaaga tatttttcgc tactaaaaaa cgtcaataaa
    27841 gtaatattgt catgtgggcg agcaatagat attatacgat tttctttttt atcggtcaat
    27901 tggctcactt tgataagtcc ttcttttata taaaggaatt tattaatttt ttcaccttct
    27961 tctactaaag tatcgccctg actatatttg acctccattt tactttttaa tagttggtca
    28021 caaattatag gcgctaggtg tttgcttact atctgattta taaaacaatc ttcacaattg
    28081 catctcaaac tatgcttatc ttggagattt gtggtaaatg tattcataat atataataaa
    28141 aatttttccg acaaatataa ttgttatttt tttaataaac gagatttttt tattctaatt
    28201 aaactatttg attctataac gtttattgtg ggtaatcaaa ttttagattg ttacaaatag
    28261 gttatttttt taccacaaga gtacgaagat attatacgaa gttccactca tcccaataat
    28321 aatttgataa ataaatgttt aagtttttca agtttatatg ttaaataatt tttatatttt
    28381 tgcaaaaatt ttaggctatg aaaaaaatcg tagagaatac caacaaatac ctcgctatga
    28441 gtaaggaaga gaataaagaa ttcttagaca acctttattc attaatgaaa aaattagaag
    28501 ccgatgtagt cgattatcaa ggtctgaaaa tactcagtgc cccaccacta tgcccagact
    28561 gtcgtagcaa tcacatcatt aaaaatggct tacaaaatgg gatgcaaaac tatcgctgca
    28621 aagactgcgg cagacaattt agagttacca ctggaacttt tgtttacagg ttgcaaaaga
    28681 aagaaaaaat gcttgattac atcagatgta tggtagcagg caaaagtctc agagcttgtg
    28741 ctaaagaggt tggtatcagc ttaccaacaa gcttcgcttg gcgacacaag atattggcag
    28801 ctttacaaag tttcgaagat aatatcaatt ttttcggtgt agtagagatg gacgaactat
    28861 tgatggatta ttccgaaaaa ggcagaatat acaaaaatgc cgaagagctg aagcgagcta
    28921 ggaagcaaaa gccaaagaaa gtagccgtat tggctgctac agacagagct ggcaacttat
    28981 tattcaaaaa atttgatggc aaaaagctaa gttcagaaca aatagagcaa tttctgaagg
    29041 ctaagatgcc aaaggatgga gtcatgtgta gcagtaataa aaaagcattc aaaaaggtta
    29101 gaagaaatgt aatcaagcat aaagaaatag taactaacca tagacaaaag cgaggtatat
    29161 atagcctaag taccgtcaag aaaaaggcta gtgaattcac gaattggata tatttcaaat
    29221 ttaggggcgt agctaccaaa tacctgcaaa actacattat gtggtatgta gtgatgggta
    29281 aatacctcac aggcaagact gtaagcaata ctgggcgaat gctcaacctc gcatcttcgg
    29341 acaggtgggc atggtatcgg tatagagagt tagtgaatat aacatatgtt aattgaaaca
    29401 tacattagtt ttccaactga ttagttttca agtttccaat tctccacatc caacatatct
    29461 taggttataa tttgggtaat caaattatag attgttacaa aaaaataact attttttaat
    29521 tatttaagtt atttttttat cacaaagtgc tatttgtagc ctttttaatt tcatccttct
    29581 cagtgttttt tgtgcattct ttgagaactt tttggttgat atttaaccgt cctataataa
    29641 ataaaccaat attaacaaat tactcttttt ttaatcttta cccatataaa acgttataga
    29701 accaactatt ttaattaatt tttttactca cttcaatttt ttttaaaaaa aatataaaaa
    29761 aaatttttaa atttgtaaat aataataggt aatttttttg aaaaaaatgg atcgtaggag
    29821 ttttttaaag caaggcttat taacaggcat aagtggtatt ttattgtctc aaatgccttt
    29881 tttaaataga ttagaaagta aaaatataat agataaaatg ataaatgaaa acgaacaatt
    29941 agctaaattt tgggaaaaca ttaatgatag caccgttaga tgtttgctat gtccaaatca
    30001 gtgtgtgatt caagatggtc agttgggtat ttgtaaagat agaaaaaaca ttaatggcaa
    30061 attatatagc ctatcttatg gtagaatagt ttcattaaat atagatccta ttgaaaaaaa
    30121 acctttatat cactttttac caatgtctac tgtactatca tttggcacgg caggttgtaa
    30181 tttttcttgt aagaattgtc aaaattggga tatagcacag gcatcaccat tagaaataag
    30241 atcatatcat tatacgccac agcaaatcgt aaaaactgca gtcgcacaaa aaatcccttc
    30301 gatagctttt acttacaatg aacccactgt attttatgaa tatatgtatg aaacggcact
    30361 tttggctaaa aattatggaa taaaaactat attagtgagc aatggatata taaatccaga
    30421 gcctttggca gaattaatac catatttaga tgctgctaat atagatctta aatcttttga
    30481 tgataaaatt tatattaaac taaatggtgg tagactacaa ccagtattaa atacaataaa
    30541 aacacttaaa aaaagtggca tttggttaga aataactaat ttgatagttc ctacttacac
    30601 tgatgacctt aatatgatag ataatatggc tagttggctc gtagataatg gctgtgcaga
    30661 aactccattg catttttcgc gattttttcc agcttacaaa ttatccaatc tcccacctac
    30721 accaataaat actattgaag aagctactaa aattgctaaa aaaagaggta tgaaatatgt
    30781 atatccgggc aatgttttgg atgaaaaagc taatagtact tattgcccac aatgcgattc
    30841 attattgatt agacgccaag gataccatac ctctattgta ggtatcatta atgatggcaa
    30901 atgtagtaaa tgcggctata aaatcacagg tgtttggaaa taaaaaaata ctaaactaag
    30961 caacaaaaat ttataaaacc ttcattaaaa catattaatg attttagata ataaaaaaat
    31021 caacctgatg tgttaatcta ttacattcac tacttctttg atttctggaa tataagtttt
    31081 tagagtttct tcgattcctt ctttcagagt tatcatggcc atcggacatg catggcaagc
    31141 acctttcaat tttaatttta ctatatagtc cgttgttaac tctataaact ccatatcacc
    31201 gccgtcattt tgcaaatatg ggcgtatctg atctaaaaga tgtataactc tttttttaat
    31261 ttcttcgtat tccattttat ttttttttaa tgatatttta tttctacgat ttttgttttg
    31321 ggtagcgaag cattgcgtat agcgatagcg cgtacagtgt tatcaaccat cttttctaat
    31381 tctcgtccat agttagtatt ttgatctaat atagctggtc tacccttgtc tgcctcatca
    31441 cacacttgag gatctatagg gatctgtccg agtatttgta tattatacat ttttgatatt
    31501 tcttcgatag aaccactacc cgaaaaaata ttatattttt tatctggagc atcaggcggt
    31561 atgaaataag acatattctc tacaattcct aaaacaggca cattaatatt aggatcttta
    31621 aacatattaa tagctttcac agcatcatta attgcaattt tttgtggcgt tgttactatt
    31681 actacacctg taaggctata attttgcaca attgttaaat ggatatcacc agtaccagga
    31741 ggtgtatcta ttaccaaata atctaattct ccccaatctg tatcttccat tatttgttta
    31801 aaagcagaac ctatcatagg gccacgccat ataagagctt tgtcaggatc tatgaaaaac
    31861 cccatactca ttattttagt tccatatttc tctaatggtg tgattatttg tttatgatct
    31921 ttatctatta tttgaggttg tatattttct aaaccaaaca ttaatggagc gctcgggcca
    31981 tgtatatcag catccatgaa tgctgttttt gctccttttt gagctaagcc tactgctaga
    32041 tttactgcta ttgtagattt acctacgcca cctttgccag agaccactgc tacgatattt
    32101 ttaatatttc ccattattga ttctgatcta acagtagtag ttttagatga gatgtttaca
    32161 ttcacttctg catctggtat aatagattta attttttcaa tgatttgcgt ttcgaaatat
    32221 ttttttaaag gacatgcagg cgtagttaat attaatgaca aactaacttt cccttctttt
    32281 acatctaagt cttgcaccat ccctaacgtt actatgtctt tctgcaaatc aggatctatt
    32341 actgatctga gtgcttctaa tattttttgc ttcattactt gattttttat tttagcaaaa
    32401 ttaataatta aaattaagta aatttataaa aaagataaat atcatataaa aatattagta
    32461 aaatttgatt atttgttact aaataaattg atatttttcg ccttcatcat caaaaaagaa
    32521 atctacccaa cctaataaaa tcaatttcat caataaagaa tagctgtcct tgtaatttag
    32581 tttaaagtat ttttgaaatt ctttaaatgt aaaaattttt tttaaagaaa atttagtaat
    32641 attttttcta aaattatcaa tatcatctat ttttacaatt tcattattat gtcgtgctaa
    32701 ccatacatct tcatatatag aactagctac gtaatttttg tcattttttc taatgtatat
    32761 acaccaatta ttatgctcgt caggagcata aataagttca tcggacgaat aataaacgat
    32821 tacttctagc acctctttat cttctatatc atgcttgata acatcatatt taatagatgg
    32881 tttacaatat agcatagctg cagtttctat catataatat tcttcttcac ttttaatacc
    32941 tacgattttc ccattatctt tgacaccaat taataatata ccaccatctg cattagcaaa
    33001 ggcagacata gaccgagcaa tttttttact atcattaata gcaaatttaa agtctaaatg
    33061 ctgaccttca ccttggctaa ttcgtttttg taaattatat tcttcgaatt tcataattat
    33121 aaatatatga aattattggt caaatattag tattttacta ttaatagtta taggttatac
    33181 gttaattacc tttttattat ttcatcaata tatgatttct attttataat ccttcatttt
    33241 gccaatttta actgcattat tggcaaaagc attaccattt tttacaaatt tgcaattgca
    33301 gatagtccct cgataatcat aagaagttaa acaatttttg taattatctc catattcacc
    33361 tataagttca tttaaaaaat tcatagtttc ataagcataa atagcataaa tagaagggaa
    33421 tgtattgtat gagtttataa attcattaac aaagtttgca atttctttat tttttctatc
    33481 taaataacaa ttattaaaaa ataaataagg agaaatttta ttaaaaaaat aaaaatcaag
    33541 attatcaaaa taaagccatg acgatggaca aaagaattct aaaaggctca aatctttaat
    33601 cgaagtacct aaatgtctaa taatattacc agctgtaggt tcatctaaag tgagtgctac
    33661 tactactgtt tgatctctaa gtcctgatgg gagtgaagag atggtatttg aaaaattaac
    33721 attattgaac ccatttttat ttaacaattg tttcaacaat ctagcattat atttctcaat
    33781 agtatctgcc gtatgaaaaa tatatattgg ggcttctctc tttacattat tagtaatata
    33841 ttcagctatt cttttcatct cttcagtgat attagataaa aattgaacct ctggaatggc
    33901 attgtctaca tctacctttt gcaagtaatg caaccacaga aatttatcag aaataaatgg
    33961 caatgtataa gataaaaact gatcatctaa aggtccaagt actatatcta catcttgcca
    34021 atttacttta gctagatttt tttcaaattc ttttttgttt tttacttgtg ctactggtat
    34081 tatttcaaaa gttgtaacat aatcaattgg caaattttgt agcaataata aagcttcata
    34141 aaattgtaaa aattcatatc cttccaaata atttatagca atattagtac ttgacatttc
    34201 catttctgac gttacttcat ctatagctgg tacaaaagct attattttta ctttatcagg
    34261 tagatgtcct tttttacatt ttttttcatt tagatcttct ataggcaatt ttaatgttaa
    34321 aatttgccca gatttaacaa aatgcgaagg tagtttattc catttaacta tgctatcgat
    34381 agaagtatta aattgtttag cgatataata taaagtttca ccttctttta ctttatagtt
    34441 aatgatatta gtagaaacat cttttgagat ttgttgtata tcatcaggga tggttagttt
    34501 tttttcagta aaacctacaa tgattttatc atttactttt agtactccag atgctaaaat
    34561 ttctttgttc caatcgcgta gatcttctat atctacatta tattctttgc taatactata
    34621 aagagtttct ttaggtttaa caatatgaaa aacagtatca tattgaactg cttttggtgt
    34681 agtttcagtt ttaataattg gtatttttag tttttctcct ggttttattt tttcttttgc
    34741 ccactcatta tgtttatata cgtcgtctaa gctgacatta taagctttag caattgaata
    34801 aacagtttgt cctgcttcta cagtatgaat ataaaaattt ttaccatttt cttctattat
    34861 ttctgtgcta ataattattt gttgtgcatt taatattgaa aattttatta taaatatcat
    34921 taaaatacaa aataattgat taattttttt cataatacaa ataatatttt tttacaaata
    34981 taatacatta atattaaata atatattatt attaaaaatt aactaatcaa aaaatatcat
    35041 aattaaacta cgtaaataaa ttccaatatt aaagaatcaa tgagaaataa aaacatctag
    35101 cggtaggaga taagttgata tgaagtaagt aaggaaaaga tgagtaatca ctaaagattt
    35161 aaaaagctta ttagattgta ggatgctaga tttatttttg cataacatca ttatatcttt
    35221 tctaacaata caatgcaata agctgcaata gatttacctc tacctatact atctaatttt
    35281 tcatttgttt tagctttaat agataatttg tcttttgaaa tatttagaat attgcataga
    35341 tttttttgaa tcatttctat atatggtgct aattttggtt tttctaagat aataatacta
    35401 tcaatatttg aaattttgta attataaggt tcgcaaattt cgattgtttt tttaagtaat
    35461 tcaacagaag caatatcttt atacgctgga tcattatcag gaaaatgaga gccaatatct
    35521 cctagattta tagctcctag catagcatct ataatagcat gtatcaaaac atcaccatca
    35581 ctatgcgctc tacatccata tttataataa atctctacgc cacctaaaat taatttttta
    35641 tttttaacta atcgatgaat atcatatcct atacctattc tatagatttt atccactatt
    35701 taggtttgtt ttgtggttta aaaaaattaa aactcattgt gaagcgtaac acattttcta
    35761 atggatgatg ctgctcgaat ggtactaaat aagagaagct caaatcgaaa atattatatc
    35821 taacacctac accaaatgta gcaaatttac gatttccttt tgttttatgt tcattgaaat
    35881 atcctgctct tacaaaaaat tgttcagcat aattatattc tacacctcca caaaaaataa
    35941 cttcgtgtaa ttcttccttg aaaccatcag gggcatccca aaaagagcta aatattgcat
    36001 ctactacact tctatgagga tccttacctt tagctattac atactcttca ttgatagaat
    36061 cgtaggcata ataaggcgga gtgggcacca ataatttatt caaatcaaaa gcaactgtaa
    36121 tggcattaaa atcatcaata ttataagtga aactaggtcc aatacgtagg ttggtaggca
    36181 aaaaagctct atagcctatt tcgctataag taattttagc accaatatta gatatatcca
    36241 taccaaaagc tatacgagca tcttgcttat ttattttaaa gtcatttttg taaaagatcg
    36301 caatatcgcc tgcgacagat tgtcctggtt tagtttcagc atgtcctgtc cccacagtag
    36361 caccccccga gagattagaa taaatatatc taagagcaat agcaaaagaa aaattatcac
    36421 caaataatct attataagca acatcaatag caaactcagt aggagaaaaa gtacctaaat
    36481 tttcatcagg agtcatacca gtaaactgga tttcacctag agtaaaatat ctaagcgaag
    36541 ctgcaatagc ctgctgttta tcaaacttat aatacccact cacataagct aaattaatat
    36601 catgaataag ctttcgcatc caaggcgtat atgaaatagt aacaccataa tccccatcaa
    36661 taaaaatata tttagcagga ttccaatgca tggagttaat atctggagta gtagctgcac
    36721 caacctctcc catagatcca gctctagcat caggagctat taataaaaat ggtacagcgg
    36781 tagtaatagt atttaatcta tcaccttctt gtcctaatat gtcaggatag ctttgtgatt
    36841 ttaatactga tatattaaaa aagcatatta atgttaaaaa actaatttca atttttcgca
    36901 tattatttta ctagcaattt gattacaaaa ttacgtaaat tttttaattt tattatatat
    36961 ttttattttt caggtaatat taaaattttt ccagaagata atgcagatgt accattgtca
    37021 ttttctaacc aagctttgta aacataagta ccaggagtta cagtattacc atgaattgac
    37081 ttgccatccc aatatatctc tgtattagca tatgattcat tataactctc atcatataat
    37141 tttgctattt ttttacctaa aagatcataa atttctatta ttattttatt atcacaacat
    37201 gcctgattgt gctgaataaa aaatgtggtg ctcgtagtca taggattagg atatggaatt
    37261 acagtattga tagttaaatt atgcttaggt ataacttgaa aagttaccgt ggcttcagaa
    37321 gaattattaa aaacatccca agcttttaat gtaagagagt ggtatccagc agagagatcg
    37381 tgtaatggga aaatcaaata accttcttta taagaatcca aaaaatagcg aaaataagga
    37441 ttaagaatat atggatcttt gtaatcataa tctatccaag ctactaaatc atgtccaatg
    37501 gcttgaccag tcagattgat accatgctca tcactaatta atgctagtat cgttggattt
    37561 tcgtctacta tatcaccatt acgaaaatta atcgaattca aaaataaatt aatttctgga
    37621 ccttcattat ctgctatagg aatagcagta ccaccaatta aaaaatcatg agtatatccg
    37681 cttgcatctt ccatgccatt ctctgcataa agtgtcaatc ttcctttatc aaaatcatat
    37741 tgtatatcta taggcataaa aaaatcgatc ttaaattcac cattttttac agtagcagaa
    37801 cctttatata caatattggt aaaagattcg aaagttgttg gaaagctact gatgtcagtt
    37861 cctaatgtcg aaagtgtttg ttttttgtca tacattgtaa cataaatagt accattaaaa
    37921 ttattaacaa catctgcttg aaaatcttca atatgtcctt ttattgtaac aaattcaaaa
    37981 gctgataaag tatcaatggc agatatggaa ttattattga tagtatcaat aataatttta
    38041 ttataaggga gagtaattct agcggcagga tcacccaata ggacaaaatt acgtaaagga
    38101 acagcattgt tagcagaatt tttagctatt ttaaataaat caccaaatgc tggtctatca
    38161 ctattaacaa aatccatcag agtatctatt aatgcattat ttaatacagc attataagag
    38221 ctatatgcta ttctagaagt agtcatcata gttacagctc cacctttagg atttaagatt
    38281 acatattcac ccgctgatgt acgttcagga tcatcgaatc tgctaaattc acaagtagct
    38341 gttaccacta gtggcatagc acacgcatta tgccacgata atatatcaga tatctgcaca
    38401 attctttcat gagctaaccc ttcttcgcct ccatgaccta tataattaaa taatagcata
    38461 ccattattca tagcatcatt aatagcacta tttacttctg gcgctcgctg gccagcgggt
    38521 gtagatattt gtgggtaagc atctaaatat attttttgaa tattcaattg aggtgcttta
    38581 gtctctatta tattggctat attttcagct tgattaatgt atgtatttcc atcttcgtca
    38641 tcagctatta agactactcg atttctccag tcacctaatt gcggtaccaa gcattcagca
    38701 tcatcgcaaa tagtgatgcc tgtattagct ccttgttttt tgtaatatct ttctattttt
    38761 tctaccattg cccatgcttc attttctgta tttacaggaa atctacctac tgctacatcc
    38821 aaagctccat agcaatcata cccttcgcca tcatctaata aggcaaaaaa atcatcagac
    38881 gcataactag ctgtgggact aactgatgca agagattgaa atgttggtat tagattagta
    38941 ttatttttaa ttctattcaa ataatcataa gaagcatcac caaataacaa aacatattta
    39001 ggtttttcat cttcattaat ggccctatca taaaacattt tgataaaatc tctaatagcc
    39061 gaaatatctt gcttgccact actaaattca ttataaattt tatcaggtga tacaatgata
    39121 tagctaagct cttcttcata cttatgtaaa tcacctattt tttttgccat tggataaaat
    39181 ttatcaggag ctataatgat gtaatctact tgagataaac catgtagatt ttgattttct
    39241 actattcctt cataatttgg tgtgtaatag ttaccattaa tagctatata ttcttttaat
    39301 ttattagtac tgtcagtaaa ctgaaaaaca tttgcattat gtaaaccaga aatttttgaa
    39361 ggatgctcag gcgtactaat atcataaaca gaaatagtat taggagtatt agatatttgg
    39421 tatttagcaa ttttattttt tggtaaaaat ggatgactaa agctcatttg attaccataa
    39481 aactttaact cacgccaagc taacacttcg atgtaatcga gccatcctac agcactatta
    39541 ttgctaccct tttgatatct caaagttata ttaatatctg gtgtcgatga tttatatatg
    39601 atattagcct ctcccttttt agcataatca tctaaatatc catgtatagt agatataggt
    39661 atattataat tgtaattatt ataagtgagt ctaaatgatg aaggaaattc tgatctacca
    39721 taaacaatgg tacgtatata aacatttgaa tcagctataa tgtttggaaa agaaaaatta
    39781 taattataca taagtgtgaa atcaaattta tcgcccaccc atagtttacc acttttattg
    39841 aaattgtata attctttttc tgaaagagca taatccataa aagtagtgac attgatatct
    39901 tcattatcaa taggagtatt agctaaattg atacgttgtg ctacaccttc gttacttata
    39961 gttacaaaaa cgaagttctt ttcactatac aaatttctcg aatgtacata cctattatta
    40021 ccatggtcaa aatcccaata tataggtgct tctacataaa atatagcata atcacctgga
    40081 ccaaacatac catcaccacc atcatatact tctattgaga tttcgtgcaa atcatcatat
    40141 ctaaaatcat tgttagcttc aggcaacatt ctactaccgt tacaaaaaac tcttaattga
    40201 tcagatggta ttgaagatga tattagcccc catgatagta aattctcata attaatacag
    40261 tgaaatccct tgtgagtagt actgagttta atccatttgc ctttggataa aactgactca
    40321 tttgtataag ctcttttttg agtaattaaa tcttcagtat atggtgacac aatagttttt
    40381 atctcccagg taaataaaac ttctataagt gtatcagata ccttgcgaaa tggacataca
    40441 aaaaatataa tttgtttttc accactaact atttgctctt gataatatat ttgaaaatca
    40501 ctagcgatat tatgtgaata ttttgataat tgattcgaag acacaaaata cgtactatca
    40561 atgtttaaat aataatccca tttactattt acaggaattt ttttagatgg cattatataa
    40621 tatggtaatt cagtaccatt gaaatattcc aaaacatatg gtttatttaa tatttcttgt
    40681 gaaaaaataa atataggtaa aaaagtaaag ataaaacaaa ataacttatg cattagataa
    40741 tttttaaata ataactgata aaaataaaat ttattttatc tgcaaaaata ttgaaaaata
    40801 tttattttaa aatttaattt tttttaatct ctctttaaaa ttaataatta ttcataaaca
    40861 agtaagataa aaattaagaa aaattttcat ttaaaagtgg atataatttt taatatcata
    40921 aacattattg ataaattaat agaaatgatc gtttgtcaaa tattccaatc aaagatatta
    40981 atatatatat taacataaat gcatctatgc tctccaaagc attaagtaat gtagagtttc
    41041 tttatattta actttttaat agtatgtaaa aatttaaaaa agagagtttg ttaaagatta
    41101 caaaacaaat cattgtttta cttgaagtag tttatctata tttcgtattc accagccgcc
    41161 aatactcatc ccacgcccat ctgtccgagg atgcaaggtt taaaagccga ccaatgtcag
    41221 attctatctc acttgttaag tatttatgtg tgattacaaa ccacatcaga tagctctgca
    41281 agtatttggt agccactcct ctaaatcgtc gcaaccaaac cttgaaatca tctatatgct
    41341 tatcgactac ttgtacactg tatacaccgc gtcaaattat ttaattatgc tataaaatac
    41401 gtaaagtaaa acaaaggata tttttatgta gctctgttca caaatcgcgt ttacgaagcg
    41461 caatttttac aaagctaaat ttcctatcca tttttatttg a
//
LOCUS       DAOPRN010000011        40127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_759_length_40127_cov_124.893966, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000011 DAOPRN010000000
VERSION     DAOPRN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 40127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40127
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_759_length_40127_cov_124.893966"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKI65_02300"
     CDS             <1..324
                     /locus_tag="PKI65_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOB77306.1"
                     /translation="DIIAWQKAKDMTVFIYKDFADSKDFGFKDQIQRSSVLVMNNIAE
                     GFERKTNNEFKQFLFIAKGSCGEVRSMLYIAKELNKISESSFNKNVELTEEISKILSG
                     LIKTL"
     gene            488..697
                     /locus_tag="PKI65_02305"
     CDS             488..697
                     /locus_tag="PKI65_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77307.1"
                     /translation="MLLPKIIGRFKINVAKQINQICQTSGIPVWQSNYYEHIIRYTND
                     LSRIRHYIADNPKNWKNDEYNINQL"
     gene            803..1042
                     /locus_tag="PKI65_02310"
     CDS             803..1042
                     /locus_tag="PKI65_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77308.1"
                     /translation="MKNYLLLFLISIPIILNSQMNVNIHEAENWGQYVVNKYFPKNIK
                     YEVISIQTISNESQIQYYVCHVYPVGFVLVSGNKL"
     gene            1088..2836
                     /locus_tag="PKI65_02315"
     CDS             1088..2836
                     /locus_tag="PKI65_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOB77309.1"
                     /translation="MQQTSIALFINQVKKIEENNKTADIHPLWNNNLDKYLNNEYRSV
                     DPLILTKWDQGIYYNQFCPKDPAGPDGRCVTGCVATALAQVINYFRWPNVGEGEYTDI
                     DTTYGHLYVNYAEQNYFYNEMPLRATRENQELAKLMYTIGVACDMYYGPHGSGMYNHK
                     AAYALRNFFRYDPTTEYLFRDETHLDWDSLLRDYLDQKIPLYYGGWSDTEYVSGHAFV
                     IDGYQDTMVYHVNWGWGGAYDGYFNINNLTPGGSDFTYKHEVVINMRPNGQYPVYCNG
                     TDTLKTLDGTIDDGSGPFYQYLPNTHCSWLIQPFDSIENIKLDFLKLDLPDPNDFINV
                     YNGDSNSGILVATITGTDIPNTITVNGKSAFIEFISNDNEEGDGFLLSYSTTVHTFCT
                     ANITEIVADSGVITDGSGPYNYRNGNFCRWKLLPANAQQLVLTFNLLDIDSTDNLRIK
                     NYDGSIDMEFKGNTLPPPILVSSFPVNIYFTSSATYSAQGFEIIFRSSLESINDYQYL
                     VSIYPNPFSNAIFFDFVTDGLLKIYTSDGKLMFSNYYNRGSYLINTDEWASGIYFIHF
                     NDNNNFFSKKIIKIYE"
     gene            2829..3188
                     /locus_tag="PKI65_02320"
     CDS             2829..3188
                     /locus_tag="PKI65_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HOB77310.1"
                     /translation="MNSLDNVILISDRVLIKPLTEEEKTASGLFLPPGYHEKEIVAYG
                     YIIKVGPGYPLPSESFEDEYWKRETSEQVRYLPLQVKEGDLAVYLQNQAIDVKIDHEI
                     YKIVNQNSILLVEREDF"
     gene            3218..4645
                     /locus_tag="PKI65_02325"
     CDS             3218..4645
                     /locus_tag="PKI65_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOB77311.1"
                     /translation="MKNIFISLIILFLPICNIYTQIDKLRLENGNYILKPADTLLLDC
                     KNDKLSKYDNKINCLSVKNQSKCSILIDKIKQIDIYNTAKVKIIDTLKTDYLKITIQG
                     ASNCEALIICDSLEIIATNASNIKLKGVVKNLNVKADESSYIDTYELSVWDATVEANN
                     FSKINIKAFNNLYITANEASIITYYGTPKILNINKSKAAVVNKSPKYIDTKNDSIKLF
                     ENFILNIDFIQIADVDTIDTMKKRNYDNDDFVMFNYKKKGSKKKKSSKSDFIGHWRYF
                     DILFTNYTQKPLSLSVPKEYNSFELDFDKSIGLQLNFLQISISLNQQNNMGLVTGLGI
                     QWVNYILKNKGYIVENDCSGLSFCTNINIFFDDDIKYTKLSAIYLEIPFLFEIQNKSN
                     PKSHFNIGPVYGFQIANLSKTKFKKSDETKYFLKSFNKYKIGLILKIGINNISWTFQY
                     NFTKLFKENRGPEVYPLELGLSIRL"
     gene            4756..5697
                     /locus_tag="PKI65_02330"
     CDS             4756..5697
                     /locus_tag="PKI65_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOB77312.1"
                     /translation="MEYKDYYKILGVSKNSSTEDIKKAYKKLALQYHPDKNPGNKEAE
                     EKFKEINEAYEVLSDPEKRKRYDELGANWDKYKDFDFSSYRPSGRTYHYTTEDLGDIF
                     GQGGRSPFSDFFDMFFGSDFNWDDIFNQSYETQTSYRPQDFRTTSRQPQYTTDLNISL
                     EEAYHGTKKILNINNKQIRVPIKPGIKDGQVLRIKRDNHKIPNVDGDLLINIHINKHP
                     KFERKGNDLYTNLELDLYTALLGGEVIVPTLDKPLKVKLKECTQPGEILRIKGKGMPL
                     YENPNRHGDLFINIKVNFPKKLTPEQRKLIQKLKNNK"
     gene            complement(5725..6399)
                     /locus_tag="PKI65_02335"
     CDS             complement(5725..6399)
                     /locus_tag="PKI65_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOB77313.1"
                     /translation="MKDKKKKFYLGERLIIFLISFTFISCTGIVEYVKQRQIETHNKG
                     DNVSKTNMATKNAISKDKEDELKTTDDAKNLNEAVISFYENKWGIELPSDADTNLIKE
                     IDRWIGVPYKYGGKDENGTDCSGMVMTIYENVYGFKLNRSSYDIIQNVIKVPVEEARF
                     GDLVFFKINNSNVSHVGIYIGDNQFVHASTKAGVRIDSLEMPYYQKRFYMVGRIKEDF
                     FKNNKN"
     gene            complement(6609..9440)
                     /locus_tag="PKI65_02340"
     CDS             complement(6609..9440)
                     /locus_tag="PKI65_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOB77314.1"
                     /translation="MKKLFCFIINLLFIIETNLLFGQTTTNYSIFDGRKEIYIAIPNK
                     NNNIDIDVLSNTIYIDKITTDTIFAYVTINQYEGFNNLNLNFIKLTPPSMRLNESELN
                     IKDADDILSKQITSWDYYPTYSAYLTLMQQFALNYPDLCRIDTIGTSVEGRLILSAII
                     SDNVHQNEKEPKVFYTSSMHGDEVTGYVLMLHLIDYLLQNYNTNNRIHKIVDSLEIHI
                     NPLANPDGTYASSNNTVSGATRGNANSMDLNRNFPVPNGSIGGDNTYSIEVETQAFMN
                     YGKKHPFVMAMNIHGGAEIANYPWDYKTQDHADKTWWIYICKEYADTAQYYSPSGYFE
                     YQPSGADYPGVIEGASWYLVSGSRQDWMNYFAHCREITLEISNTKTPPASELENFWTY
                     NYRSFLNYLEQAIYGIRGTVIDGCTYEPIKALITINNHDTDSSQVYSHLPHGDYYRPI
                     AAGSWTMIVSAPGYQSDTITSITIENKSIAMRNITLYPYPPVADFTVDVTNTCTGIVN
                     FINNSTTSVNSLFLWDFGDGTTSNESNPTHIYTQNGTYNVKLKVTSCAGKDSVIYNNL
                     ITVEMPDSPVLSQTNYNVCSGDNLTIEATANGNIYWYNDSLANSIINTGNQYYISNIL
                     NDDTIYVNNVISHTYNGGKLDNSGTGGYFNNYVEHGLYFDCLQSVNLKSVKVYANSTA
                     DRTITLYDSNSNALYSQTINIPSGESRISLDWQIPQENNLKITASTYPNLFRNGSDNG
                     PNLGYPFNINNYISITSSTAGTSATSYYYYFYDWEIEEKCESPLIPIYINVNELPIAN
                     FTYNIEGNIVSFFNYCQNANYFYWDFGDGTNSTEINPIHIYNNSNSFIVSLIASNECG
                     LDTYTQTITITNIDDFANEIINITTIDNNLIIKSESIIENVVITNVIGQIIYEKDINS
                     NYCFIPLQAFNQGIYLIKINNKYFYKIAK"
     gene            complement(9445..12093)
                     /locus_tag="PKI65_02345"
     CDS             complement(9445..12093)
                     /locus_tag="PKI65_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOB77315.1"
                     /translation="MKKILFLILFLVSLISYCQISYPGIPASFDHNIKKTNIYILPHI
                     DVEVLKMEDAIVDQYKDRPWRFGQNVYVDINIMDQAFDTIINGKKIWLMTFKSDGAIT
                     LNFTFDQFYLPENTELYLYNADKSMVLGAFNHNNNSEDGVFATTLIPGDEVIIEVNQD
                     AAINNLPTLHLYRITHGYRNAYEYALKSFGNAGSCNVNVACPQAAGWEDQIRSVCMLV
                     SGGDGFCSGALINNTNNDGTPYVLTANHCYSDPSSWVFWFNWQSPTCTNPVSSPPYNS
                     MSGATLKARYANSDFCLVQINNNIPSTYDVYFSGWNRTMDNNISGKVVGIHHPSGDIK
                     KISWSNNGVSTTTYLNNSIPGDGTHWRVTSWSDGTTTEPGSSGSPIFDPTGHIIGQLH
                     GGYAACGNTSSDWYGKLGVSWDGGGASSNRLKDWLDPTNSSVTILEGYDPNTPVADLD
                     ASVIQILPSNNEFCEISTQNFQAVIKNRGNLTLTSLKIYWTFDDGSTNLINWTGSLAT
                     NQTTTVDLASYLLEEGIHNLKVWCANPNGGVDENLNNDTLNWQGTVYPSLRPSPFVEL
                     FNSSFPPACWAVYIGTNGLGTSYNWQSTNEAYEGYGAAFVRYEIVYGGQTEDWLVTPK
                     IHLCDDPVLSFYQKQDFSTNYGSTYSVRVSTTSQTSHSSFTTVSEWNESSFSTSYTKK
                     TIDLSAYADQNVYIAFVMAQNNGDNWYIDSLLIKGQCDPNLTLDLGPDMDTICGRQIT
                     IDAGDEFASYHWSDESIGQTITLIPQNGQNYHYSLTATDYCGCTHTDDINITCHINEA
                     ISDYDNDFSLYWSGDYQALIFNNKSNDQISISIYNPLGMRIVENEIIEANSVKNIYLS
                     NIANGIYYSHITVRDGSIKIIPFVKY"
     gene            12185..12258
                     /locus_tag="PKI65_02350"
     tRNA            12185..12258
                     /locus_tag="PKI65_02350"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12219..12221,aa:Ile,seq:cat)
     gene            complement(12898..15387)
                     /locus_tag="PKI65_02355"
     CDS             complement(12898..15387)
                     /locus_tag="PKI65_02355"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOB77316.1"
                     /translation="MRQYRAIELANIINAVFFSQKNDIQNVFIDEIVFDTRLPINKPS
                     TAIFFALKGLQDGHLFIDDAYNKGIRNFCIKDKKYIKKYENANFFIVDNTLDALQNWA
                     KYHRKQFNIPIVAITGSNGKTIVKEWLAISLANKFEVAKNPRSYNSQIGLPYSILQLE
                     SKHQIGIFEVGISQPHEMDKLADIIKPDTVIFTNIGPAHQQYFKNISEKIKEKLKLAI
                     NAKKLIVCSDYDQIIKEARSLSINLITWGTKSTDNIKIINKSTNKWVTEIELIYDDKT
                     YNFSLPFIDTASIENAMHVITYCLTNELMANEIQNYIPQWETISMRLETVHGNNGNLI
                     VSDFYSNDLLSLSISLQYLNQKSESKKKIVFLSDILESDESIEVLYEKIAKELDKNKV
                     DEVYTVGDNIIKLSSYFHRVKGHFRSTEECVMKIEPDQFKDSLILLKGARKYTFEKLL
                     PFLVENNSYAELRVSLNAIQHNYQYFHSIISKDTGIIAMVKAFAYGSGAEDISSFLEQ
                     LGVDYLGVAFSHEGVSLRRSGIKSPIIVMSPHLSSLQMCIKYNLEPEIFSFLALEQMI
                     QVVKEYSFLAPIPYPIHLKIDTGMHRLGFLPEQYTMLSKRLQEVKDLIKVSTVFSHFS
                     ASDMPEHDDFTKLQAKKLNDFTEKLQNDLSYKIKKHICNTHGTIRFPEYHWDYVRIGL
                     GLYGITNIDEINKKLMPAAELSSFVTQIREINARESIGYNRKAISEHSRVIATIPIGY
                     ADGIYREMGNGNGFVKINDKYAPVVGDICMDMMMVDVTSIWPNINEGDKVIIFGDDIS
                     ILDYAKVNKTIPYHVMTSISPRVKRSYIFFF"
     gene            complement(15365..16426)
                     /gene="pdxA"
                     /locus_tag="PKI65_02360"
     CDS             complement(15365..16426)
                     /gene="pdxA"
                     /locus_tag="PKI65_02360"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOB77317.1"
                     /translation="MNNVDRDFLIAGITPGDLNGIGYELIYKAFKNIQLFDICTPIVL
                     GNPRAAAYHRKMINNCNVNLFLLKNLKNLKPGKPYIYPVSSIEYKIEIGKITKVGAEA
                     SIESLEQGIKWFNEGKIDFLVTCPINKKALSLIDFPFKGHTEFLADAFNIQEYLMMFI
                     NPYLKIGLVTTHAPISELKNILNAHLILSKIKVLDNSLQRDFGFIKPKIAVLGLNPHA
                     SDEGLIGNEENDVIIPAIREANEIGIEAYGPFPADSFFGNLRYKDFDAVLSMYHDQGL
                     IPFKLLDNQEAVNFTAGLPIVRTSPVHGTAFDIAGKDKANERSFVQAILLGAEITLRR
                     RYYDENIKAQLKDETVSSN"
     gene            16725..17726
                     /locus_tag="PKI65_02365"
     CDS             16725..17726
                     /locus_tag="PKI65_02365"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOB77318.1"
                     /translation="MINKETISEWRDNVIENLNFATFDFLLKDIEKENPTFTPKFVKT
                     AIKNIFYALTPEKYYNWITKYNIPNKTHSLGIICAGNVPAVSFFDVFMGLVTNHEVYV
                     KLSHSDTKLIPYLFEQLFNFIPEMRDRIKFVESIHSVKAEKIIATGSNNANRYFLQYF
                     ADKKAVLRHDRKSIAVINHDITENELNALANDIFLYFGLGCRNVSLILLPHEFSLNKI
                     NAAFKHYSWISDNLYYKEIINYYRAYFSLMFEDFIDNDFYLLRERLDLYSPISVINYA
                     YYNDISQVENFIKANKENLQCIVGNYSLCNTTFGKTQKPELSDYPDDVDIMRFLTES"
     gene            complement(18029..20254)
                     /locus_tag="PKI65_02370"
     CDS             complement(18029..20254)
                     /locus_tag="PKI65_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOB77319.1"
                     /translation="MKRFFASLLLLLFVTQASFGLKPPTRPDEGMWIPVWIEELNYPE
                     MARLGIKLSAEEVYSNTQPSIKDAIVGLGTSTYTPNLGFFCSGVVVSPQGMVFTNHHC
                     GYDAIQKVSTLENDYLTHGFWASKLEEEIPIEGMTMSFVVKIEDVTARILRDIDDSMS
                     YSSREKAINKAKKGIENEASEKGKYTAVVKPMFEGNEYYLYVYETYKDIRLVGTPPNA
                     IGKFGGDTDNWMWPRHTGDFSIFRIYTAPDGSPAEFSKNNVPLKPKKYLPVSNQGVEK
                     DDFAMIFGFPGSSNRYLTSDGVNIAINESNPLIVNIRDKKLEIMRSYMNKDPKIKLQY
                     ASKYAQISNYWKYYIGQTKGLKRWDVYNRKKELENQFINWVQQDQNRQQKYGTCLQEI
                     KESYELIQNYNKTITYLEEAILQGPEFIYFGFDFLQLYALLKNLNNVDKNDKPKIEAN
                     IKLIAKDLSEKAKKHFKDYNVNVDKDIFIAMFEMYTSEVPKEQQISAIKTLEKKYKGD
                     IKRWADDIYRKSILVDETRLDNFLQNPKLKTLENDLGFQITLNTLSSLREIWQEMSRA
                     EAKQKEGYRKYIAGLREMMPEKAFYPDANSTMRMTYGTVQDYFPADAVHYDFITTLKG
                     VMEKEDPTNEEFIVPDKLKELYKSKDFGPYVDKNGNIITCFITNNDITGGNSGSPVID
                     ANGHLIGLAFDGNWESMSGDILFETSVQRCINVDIRYVLFIIDKYAGATNILNELTII
                     N"
     gene            complement(20865..21353)
                     /gene="nuoE"
                     /locus_tag="PKI65_02375"
     CDS             complement(20865..21353)
                     /gene="nuoE"
                     /locus_tag="PKI65_02375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOB77320.1"
                     /translation="MSHIKIKLHESQLNFIKDTCKEFNYDPGELINILHKLQDQFGYL
                     PSEVQEAVASELKIPTAKVYGIVTFYSYFTMIPKGEYPINICLGTACYVRGAEKILDE
                     FRKQLNIKVGESTPDGKFSLNCLRCVGACGLAPVVMVGEKTYGRVAPDDVKKILSEYQ
                     ND"
     gene            complement(21372..23147)
                     /locus_tag="PKI65_02380"
     CDS             complement(21372..23147)
                     /locus_tag="PKI65_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOB77321.1"
                     /translation="METVHITINNIPLEVPKNTTVLEAARMINIEIPTLCHMKLEDFD
                     IENKPAGCRICVVEIKGRKNLAPSCVTECTEGMVIETSNPRIINARRTVLELILSDHP
                     FECLICSKNGKCDLQSLAQELGVREIRYTGEQSTYRMDTSQAIIRNPDKCIMCRRCEL
                     MCNTVQTCGVLSAVNRGFTAVVAPAFEQNLDHSPCTYCGQCVAVCPTAALTEVDQTGK
                     LIQALADPDKVVVVQTAPAVRVTLGEEFGIKPGTIVTGKMVTALKRIGFDYVFDTDFA
                     ADLTIMEEGTELLDRLTRYLNGDKSVELPILTSCCPAWVNFFEKNFPDLLDVPSTAKS
                     PQQMFGAIAKNYFAKQLNIPREKLVVVSVMPCVAKKYEAQREEFRVNDDPDVNISITT
                     RELATLIKQTVQDFESLPESDFDKPLGESTGAAAIFGTTGGVIEAAVRTAYELYTKKP
                     LPKIEFEELRGMEWIRKATIDFNGLKLNIGIAHGLGNARKLLEEIQAGKSEFHAIEIM
                     ACPGGCIGGGGQPQHHGKSEIIKARQKAIYEEDRRLPLRKSHENPYIIKLYEEYLGHP
                     MSELSHELLHTQYFKKQPEIIIEND"
     gene            complement(23170..24963)
                     /locus_tag="PKI65_02385"
     CDS             complement(23170..24963)
                     /locus_tag="PKI65_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOB77322.1"
                     /translation="MGTFKYHILVCGGTGCKSSQSAQIVQNLNEEIKENQLEDFVKVV
                     TTGCFGFCERGPIVKVLPDNTFYNYVKPEDAKEIINEHIIKGRRVERLLFRDPVTKEP
                     VTDSKHINFYQKQLRVVLRNCGVIDPENIEEYIARDGYLALYKCLNEFTPSQVIDIIK
                     KSGLRGRGGAGFPTGVKWEITASNPGPEKYVVCNADEGDPGAFMDRSILEGDPHSVLE
                     AMAICGYAIGASHGLIYIRAEYPIAIKRLQIAIEQAKEYGLLGKNIFNTDFSFDIELR
                     YGAGAFVCGEETALIHSMEGNRGEPTVKPPYPAVAGYRGKPTNVNNVETFANVPPIIL
                     KGPDWFTSIGSEKSKGTKVFALAGKINNVGLIEVPMGITLREIIYEIGGGIKNNKKFK
                     AVQTGGPSGGCLTEKHLDTPIEYETLVAAGSMMGSGGMIVMDEDDCMVSVAKFFLDFT
                     VEESCGKCNPCRIGNKRLSEILDKITKGNGTMEDLELLKNLCMVIKDTALCGLGQTSP
                     NPVQSTLNNFWDEYVEHITDKKCRANKCKDLIHYFIDPENCTGCMACARKCPVNAIAG
                     ERKQVHVINQDLCTKCGICLETCKFNAVYII"
     gene            complement(24976..25374)
                     /locus_tag="PKI65_02390"
     CDS             complement(24976..25374)
                     /locus_tag="PKI65_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOB77323.1"
                     /translation="MAKITSLDDLRKIKKNQEDQINLREKAEHPESLVQVKVGMATSG
                     IAAGAKEIFTFFVDELEKRNIPAVVTQTGDMGYSYAEPTVEVKLPGQEPIVFGWVTKE
                     RADEIIEKYIRNGELIEGIIPQNYKTIDDK"
     gene            complement(25578..26123)
                     /locus_tag="PKI65_02395"
     CDS             complement(25578..26123)
                     /locus_tag="PKI65_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOB77324.1"
                     /translation="MKEIVDHIIDIVNNSISAGAQNITIQIEESIKNDYLKLTIIDDG
                     KGIDTDTIKIITDPFFTTKKDKKVGLGISLLKYHAELSEGKFQIKSELNKGTEVIASF
                     KLSHVDRQPLGDIAGTIILLIMAHPNIHFVYKHKTDFGYFVLDTLDIKDIINDICTNG
                     IMRRSLKEYIDENLKQIKAMK"
     gene            complement(26120..26893)
                     /locus_tag="PKI65_02400"
     CDS             complement(26120..26893)
                     /locus_tag="PKI65_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOB77325.1"
                     /translation="MMMCDLLYDFIADLHIHTVLSPCGSLDMSPINIVKEASKKGIDI
                     IGITDHNASANCKVTFDIAKDYGIFTLCGLEVTTKEETHCLTFFQDFETLDLFQKFID
                     DNIIKIDNDPDKFGYQLIVDKDENIVDQIDWLLINATNISIDDLEKKVHQLNGLFIPA
                     HIDRSAYSLISQLGIVPKDLNVDAFEVSKYSNPQNIINSFPYTKKNTFISDSDAHFIN
                     DIGCITTIFHIKNRSFHEIKKSLYKIDGRDVCINYNDNI"
     gene            complement(26880..27227)
                     /locus_tag="PKI65_02405"
     CDS             complement(26880..27227)
                     /locus_tag="PKI65_02405"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOB77326.1"
                     /translation="MKVADILKWENTKIVCCENSLNNQITDGYVSDLLSDVMANANEN
                     MAWITIQTHKNLVAIASLKELSCIIITNNNTPADDLIEAAKQENIPIISTSMSSFDTA
                     GKLYVALHFNDDV"
     gene            complement(27214..28581)
                     /locus_tag="PKI65_02410"
     CDS             complement(27214..28581)
                     /locus_tag="PKI65_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOB77327.1"
                     /translation="MSKNQIFSHALEIDRSLCVGCTHCMRVCPTHALRVRNGKAVLYP
                     DRCIDCGECFRACPIGAIKIKEDDLSALDKYKVKVALVPSVMFGQFPDNISIKQIYKS
                     IYELGFTHIYEVEHSIDFLRKQIINYQKKSNIKPLISSFCPAIVRLIQVRFPSLTDNI
                     IKLRTPKDLTAEIILDNFTNQGFSADDIGIFYITPCAAKIAAIKSPVGEDKSVINGVI
                     NMNSFFNMIMNKCKSKQYSDLELLDIQPSKLLPMAITWSLTRGEAQYVKGRSFAVDGI
                     LNVCDFLEKIENEEIQDVDFLELRACDESCAGGILCTKNRFLTVENLYKRASQSIKKK
                     KNIKLNKEIEKLLIDKISITNIEPRPMNLDNDLEKAIKKLERIRDIMCFLPNVDCGLC
                     GAPNCKTLAEDITNNVAHISDCVFIQYKNLTDPEQAKKILAKIWGENVFDKDCSKKGA
                     KYEGS"
     gene            complement(28585..28995)
                     /locus_tag="PKI65_02415"
     CDS             complement(28585..28995)
                     /locus_tag="PKI65_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOB77328.1"
                     /translation="MILTYKVQGGDFSNAGKCSSEVKKVLKQLNIDPKIIKKVVIALY
                     EGEVNIVAHAYHGVINVEIDTEKIKITLKDQGPGIPDINKAMEQGYSTASPIVRELGF
                     GAGMGLPNMKKNTDYMNISSHVNEGTEVILINYF"
     gene            complement(29003..29341)
                     /locus_tag="PKI65_02420"
     CDS             complement(29003..29341)
                     /locus_tag="PKI65_02420"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOB77329.1"
                     /translation="MSKIVEAVDGTVYTCKEYLDDEVEYGFSSDLMSDVLTIDKEDVI
                     LITGLNNIQTIRTAELAEIKYVIYARNKMINNDIIKLANENKILVIQSPYSVFKVSGI
                     LYNLGVKPVY"
     gene            complement(29541..30593)
                     /locus_tag="PKI65_02425"
     CDS             complement(29541..30593)
                     /locus_tag="PKI65_02425"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOB77330.1"
                     /translation="MKAKTKKTLLIIILAIIVIVIVGLFILHRTDPALYPRDNAFNIT
                     DTSAITKIFLADKKGNSVVLTRTSEGWLVNDSLKANNNMINEFMRTLYYVSLKSPVPI
                     TSRDNVLKRMSSFGVKVEIYARVPWARIGSLWIFPKERKIRTFYVGDNPPDKIGTYFL
                     MEGAKEPYIMYIPGMMGFLQNYFTPIEADWRDHTFMNMYYQQIHKVEVDYFDKPENSF
                     TIIKENNDNIHLYDYNNQEINNVDLPKLRSYMSGFVNIRFAEMLSYLDSAKRDSILHQ
                     QPFCHIRLISNNNATQEMTFYKIYYPDGIEDSFGRIIYEDRDLLHGYNEELGLVTCQY
                     YVVASFFQPITYFVSN"
     gene            30980..31435
                     /locus_tag="PKI65_02430"
     CDS             30980..31435
                     /locus_tag="PKI65_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOB77331.1"
                     /translation="MKIHYHSDHLGSASFVTDIGGNAVQHLQYLPYGELFVSQRKSKE
                     FDSRYKFTAKALDNETSYTYFGARYYDSELSAWLSVDPMSDKYPSLSPYCYSADNPVV
                     LVDPNGEKVYIVGDAADEATGFLIKKLYKFSQPPYNKPSSIHDQINNNK"
     gene            31440..31946
                     /locus_tag="PKI65_02435"
     CDS             31440..31946
                     /locus_tag="PKI65_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77332.1"
                     /translation="MKKLKAFCILLLLNLVVSVSILANNNLDNKINYEFFYQGKDTIY
                     YTTGEYRISRIKSIRHAYLIYLSNSTMDILYTLISPKCNVRLPEKLKEGETYIMQIIY
                     LVPTQKYARYVGHKFITKLTMLDKKVRISRRNLWGTIIISPNVLDKYYRKDHFLGPEV
                     FKEANNYE"
     gene            31939..32838
                     /locus_tag="PKI65_02440"
     CDS             31939..32838
                     /locus_tag="PKI65_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77333.1"
                     /translation="MNKILNNCRIQRLFILIIVISNNIFSQKNLIYNGSFEEYYSCPC
                     NLNVVDTIPCIGWWSPNDATPDYFNKCWIELPHTNFCLYNYIYLASYKPKTGNGYMGL
                     GLIGEENFWMEHIQSKLIEPLEAGKQYKVSFWVKLAYKYSDYAAYNIGLYFSKDSDIV
                     GKKYNVSSYVRYMTPELRAHIKNPDGNFIIDTNWVEISGIYTAQGGEQYITIGMFWDD
                     NPAVVEAHKKYKNKNTQYNKKLLSKAIKNNLLVENPYIEKYKKMEDQYIRSHSPYYYH
                     YWDKEKKNIYYFIDDVSVIELDE"
     gene            33336..35465
                     /locus_tag="PKI65_02445"
     CDS             33336..35465
                     /locus_tag="PKI65_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOB77334.1"
                     /translation="MKAYQVKDIRNVAIIGSPKSGKTTFVEAMLFDANVINRRGNVDE
                     GNTVSDYRDIEIERGYSVYSSVLALEYENSKINVIDTPGFDDFIGEILAALKVCDINL
                     NLINAANGIEVGTEILWRHLDRLNKSAIFVFNQLEHEKAQFQEIVNDLNNSFSNRIIP
                     FQYPLNVGPDFKEVVDVLSEKLIIFTDSNGNYTEKDLPDSEKSKVADLKNQIIELVAE
                     SDEKLMEKYFENGTLDYQDIQNNLKKAIISKSIFPVFCASAKLNYGVKNIMNFLVHFA
                     PAPNELPFEKAKSGRELSYNPNDPFVGFVFKTTIEQHLGELSFIKIYSGTLSEGMDVV
                     NATNGQRERISQLLVINGKNREKISQVYAGDIVATVKLRYTSTNDTLTSAKNTNDIVE
                     PIEYPEPKYRVAIRAKNTADDEKLGAVLQELHRTDPTLQVEYSKELKQIILSAQGEMH
                     ALAAKWQIETIEKIPIEFLDPKIPYRETITKSARASYRHKKQSGGAGQFGEVHLMIEP
                     YHEGMSFNVEFPIRGTEEYDLPWGGKLVFNNCIVGGAIDTRFLPAILKGIMEKMEEGP
                     LTGSYARDIVVYVYDGKMHPVDSNEISFKIAGRSAFREAFKNAGPRILEPIYEIEVLV
                     PADRMGDVMTDLQNRRGIILGMEAEGNYQKIKAKVPLAELNRYSTALSSLTSGKATFT
                     MKFSEYQQVPSNIQETLLKAYEEKDEE"
     gene            35596..36210
                     /locus_tag="PKI65_02450"
     CDS             35596..36210
                     /locus_tag="PKI65_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOB77335.1"
                     /translation="MKIICIGRNYLDHITELNDAVPQEPIFFMKHENALIKNKNMPFF
                     LPDFSDEIHYEVELVIKIGKVGKTIPKKHALDFINGITVGIDFTARDLQRKCIEEGLP
                     WEICKSFDYSAPIGSFVAIERFSDIKQIHFSLKKNDKMVQYGCSGDMIFFFDDIISYI
                     SKFITLKTGDIIFTGTPKGVGQVKKGDKLEAYLENEKMLNKIVL"
     gene            complement(36205..37284)
                     /gene="corA"
                     /locus_tag="PKI65_02455"
     CDS             complement(36205..37284)
                     /gene="corA"
                     /locus_tag="PKI65_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOB77336.1"
                     /translation="MKLNFNTITNKLPHKKAKVYLPPESLVYTGDIHDDIKIKIIQYN
                     ENGYLEVEITEVEDLTKLNFTDNITWLQIYGLHDEDLIKQIGDYFKIHNLVLEDILHV
                     DQRPKIENYDDYLFLVIKSFSAIENDLQLDQKQISCILSENYFITFLEKQTNFFDPII
                     DHIKNNRNNIKTKKPDFLLYIFLDLIVDSYFLLLQGIEDNLYTLENDLIGDNNDDITN
                     QLHKLTSNISCLHRYIFPVVEITKNIVNLNSKLIHDTTFYFRDLYDHTYRIVDSLESY
                     REMTFNLLQIYLSEVSTRMNEIMKVLTIIATIFIPLTFIVGVYGMNFKYMPELEYKYG
                     YFIVWGVMVLIAILLIFYFKKKKWL"
     gene            complement(37306..37503)
                     /locus_tag="PKI65_02460"
     CDS             complement(37306..37503)
                     /locus_tag="PKI65_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77337.1"
                     /translation="MKKRVYFISICIILLFANCGKINKKDKKEIKESVEQMNTDTIDS
                     KLIFESAENFFSSDTLLNDSF"
     gene            complement(37548..37946)
                     /locus_tag="PKI65_02465"
     CDS             complement(37548..37946)
                     /locus_tag="PKI65_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOB77338.1"
                     /translation="MKKESYNTDNKKFTIGDLLKEKGFNLTNTNNEESETDDVQSSNN
                     NELSIADLSNNSKIRVIYQRKGRANNPVTIIKGLDLDEEKIKILAKIIKQKLAVGGTI
                     DNNEIILQGDQVDKVIEFLTSLKFNDVKKG"
     gene            complement(37995..38897)
                     /locus_tag="PKI65_02470"
     CDS             complement(37995..38897)
                     /locus_tag="PKI65_02470"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOB77339.1"
                     /translation="MSLALFIVNPIAGKRKKGRVINEIKNFCNNNNIDYEFFFTTGNR
                     DDSKNFIKNFYRKDLLSVVAVGGDGTINEVAEAIVNTDIPLLVIPKGSGNGMARHLNM
                     LLNTKDILPALTNGRIIDFDVAYLNNQIFLCNAGFGFDAYLANYYSQHTMRRFLPYIF
                     AGIKALIRYKPHRYTLQLQVKKNNIVFIPFLLTFSNISQYGYGITINPNARTDDGKLD
                     VLVFRPFKWYNVLRLIILFLQKKLHKSPFLTILRISSCELEARTKEYYHIDGEPYMST
                     DVHFNIRVEKGALKCLLPPKSKYQ"
     gene            complement(39088..39270)
                     /locus_tag="PKI65_02475"
     CDS             complement(39088..39270)
                     /locus_tag="PKI65_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77340.1"
                     /translation="MRRFRGVATKYLQSYLMWFVITHKYLASEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
     gene            complement(39844..>40127)
                     /locus_tag="PKI65_02480"
     CDS             complement(39844..>40127)
                     /locus_tag="PKI65_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77341.1"
                     /translation="TKSKKVVVNKTQIRRGVYSVQVVDKHIDDFKVWLRRFRGVATKY
                     LQSYLMWFVITHKYLTSEIESDIGRLLNLASSDRWAWDEYWRLVNTKYR"
BASE COUNT        14046 a         6201 c         5565 g        14315 t
ORIGIN      
        1 gatattatcg catggcaaaa agcaaaggat atgactgttt ttatttataa ggattttgcc
       61 gatagcaaag attttggttt caaagaccag atacaacgat catctgtttt agtaatgaat
      121 aatatagcag agggttttga aaggaaaaca aataatgaat ttaagcaatt tctttttata
      181 gcaaaaggct cgtgtggtga agttcgctca atgttataca ttgctaaaga gctaaataaa
      241 atttcagaaa gcagttttaa caaaaacgtt gaacttaccg aagaaatatc gaaaattctt
      301 tcaggactta tcaaaacttt ataaacttta tgaactttac aaactaaaaa gaaagctatg
      361 ggctttagct ctgaaatata gattattgca gtttattaga tgattcacca aaaaacaaat
      421 tgtaggggca attcacgaat tacccaacaa caaaatgaat ttccacaatg gattcaacga
      481 cgtaaaaatg ttattaccaa aaattattgg ccgttttaaa ataaatgtcg ccaaacaaat
      541 aaatcaaatt tgtcaaacct ctggcattcc tgtctggcaa agcaattatt atgaacacat
      601 cattcgctat accaatgatt tatcacgcat tcgtcattat attgcggata atccaaagaa
      661 ttggaaaaat gatgaatata atataaatca attataagaa cttattgcca tacactctca
      721 ataactgtca tgctattagt taaactctta tttcaataat ttaattttct tgtatatttg
      781 taaaatatta atcaaaagac ttatgaaaaa ttatctttta ttatttttaa tatcaatccc
      841 aatcatatta aatagtcaaa tgaatgttaa tattcatgag gcagaaaact ggggacaata
      901 tgtagtgaat aaatattttc ctaaaaatat aaaatacgaa gtgatatcga tacagactat
      961 atccaatgaa tcacaaatac aatattatgt ttgtcatgtt tatccagtag gatttgtttt
     1021 ggtaagtggc aataaattat aacatccgat tttaggatac tcttgggagt ctaacttcat
     1081 ttatcctttg cagcagacat ctatagcatt atttataaat caagtaaaaa aaattgaaga
     1141 aaataataaa actgcagata ttcatccatt atggaataat aatttagata agtatctcaa
     1201 taatgaatat cgctcagtag atcctttaat attaactaaa tgggatcaag ggatatatta
     1261 taatcaattt tgtcccaaag atcccgctgg tcccgatggc agatgcgtta caggctgtgt
     1321 agctacagct ctggcgcaag taatcaatta ttttcgttgg cctaatgtag gcgaaggtga
     1381 atatacggat atagatacta cttatggtca tttatatgtc aattatgcag agcaaaatta
     1441 tttttataat gaaatgccat taagggctac acgagaaaat caagaattag caaaattgat
     1501 gtatacgata ggagtggctt gcgatatgta ttatggaccg catggatctg gtatgtacaa
     1561 tcacaaagcc gcttatgcac ttagaaattt ctttcgttat gatcctacta cggaatattt
     1621 atttcgtgat gagacccatt tagattggga cagccttttg agagattatt tagatcaaaa
     1681 aatacctcta tactatggtg gatggtcaga taccgaatat gtaagtggtc atgcttttgt
     1741 gatagatgga taccaggata ctatggtcta tcatgttaac tggggatggg gtggtgctta
     1801 tgatggctat tttaacatta ataatttgac tcctggaggt tctgatttta catataaaca
     1861 tgaggtggtg ataaatatgc gccctaatgg tcaatatcca gtttattgta atggcacaga
     1921 tactttaaaa actctcgatg gtaccattga tgatggtagt ggaccttttt atcaatacct
     1981 gccaaatacg cactgtagct ggcttataca accttttgat tctatagaaa atattaaact
     2041 cgattttttg aaattagatc tacctgaccc taatgatttt attaatgttt ataatggaga
     2101 tagcaattct ggtattttag ttgccaccat cactggaaca gatattccca atacaatcac
     2161 agtaaatggt aaatcagcat ttattgaatt tataagtaat gataatgaag aaggggacgg
     2221 ctttttgctt tcttattcca ccacagtgca tactttttgt actgctaata ttacagaaat
     2281 agtagcagat agtggcgtca taactgatgg tagtggacca tacaattatc gtaatggcaa
     2341 tttctgtaga tggaaactat tgccagcaaa tgctcaacaa ttagttttaa cctttaattt
     2401 actcgatata gattctactg ataatttgag aataaaaaat tatgatggaa gcatagatat
     2461 ggaatttaaa ggtaatacgt tgccaccacc aatattagta tctagttttc cagttaatat
     2521 ttacttcaca tctagtgcta catattcagc gcaaggattt gaaataatat ttagatcttc
     2581 attggaatcc atcaatgatt atcaatattt agtatctata tatccaaatc ctttttctaa
     2641 tgctattttt tttgattttg tcactgatgg attattaaaa atatatacaa gtgatggcaa
     2701 attaatgttt tcaaattatt ataaccgcgg atcatatctt atcaatactg atgaatgggc
     2761 atctgggata tattttatcc actttaatga taataataat ttttttagta aaaaaataat
     2821 aaaaatatat gaatagttta gacaatgtta tcttgataag tgatagagtt ttaataaaac
     2881 cgctcactga agaagaaaaa actgccagtg gcttattttt gccacctgga tatcatgaaa
     2941 aggaaatagt agcttatggg tatattataa aagtaggtcc tggctaccct ctacctagtg
     3001 aatcttttga agatgagtat tggaaacgtg aaacctcaga gcaagtaagg tatctaccat
     3061 tacaagtaaa agaaggagat ctagcagtat atcttcaaaa tcaagctatt gatgtaaaaa
     3121 tcgatcacga gatttataaa attgttaatc aaaattcaat tttacttgta gaaagagaag
     3181 atttttaaat aaaattcatt atttattaat gaaaattatg aaaaatattt ttattagttt
     3241 gataatttta tttcttccta tttgcaatat ttatacccaa atagataaat tgcgtttaga
     3301 aaatggtaat tatatattaa aaccggctga taccttatta ttagattgta aaaatgataa
     3361 attatcaaaa tatgataaca aaattaattg tttatcagta aaaaatcaat ctaaatgctc
     3421 tattttaata gataagatca aacaaatcga tatatataat acagctaaag taaaaataat
     3481 cgatacatta aaaaccgatt atctaaaaat aactatccaa ggagcttcta actgcgaggc
     3541 attgattatt tgtgatagtt tagaaataat agcaactaat gctagtaata tcaaattgaa
     3601 gggggtagtg aaaaatctga atgtaaaagc tgatgaatct agttatatag atacttatga
     3661 attatctgta tgggacgcta cagtagaggc taataatttt tctaaaataa acataaaagc
     3721 ttttaataat ttatatataa ctgctaatga ggcttctatt ataacttatt atggtactcc
     3781 taaaatttta aatataaata aatctaaagc tgcagtagtg aataaatcac caaagtatat
     3841 tgatactaaa aatgattcta ttaaattatt tgaaaatttt atattaaata tagattttat
     3901 acaaattgct gatgtagata ctatagatac aatgaaaaaa agaaattatg ataatgatga
     3961 ttttgtaatg tttaattata aaaaaaaagg tagtaaaaag aaaaaatcaa gtaaaagtga
     4021 ttttataggc cattggaggt attttgatat attatttact aattatacac aaaaaccatt
     4081 atcactctct gtacctaaag aatataattc atttgaatta gattttgata aatccatagg
     4141 attgcaacta aattttttac aaatttctat atcattaaat caacaaaata atatggggtt
     4201 agttacagga ttaggcattc aatgggtaaa ttatatattg aaaaataaag gttatatagt
     4261 agaaaatgac tgtagtggac ttagtttttg tacaaatata aatatctttt ttgatgatga
     4321 tataaaatat acaaaattgt cagctattta tttagaaata ccgtttttat ttgaaataca
     4381 aaataaatct aatcccaaat ctcactttaa cattggacct gtatatggat ttcagatagc
     4441 aaatctttca aaaacaaagt ttaaaaaatc agatgagact aaatattttt taaaaagttt
     4501 taataaatat aaaattggtt taatattgaa aataggtatt aataatatct cttggacatt
     4561 tcaatataat tttacaaaat tatttaaaga aaatagaggt cccgaagtat atccgctgga
     4621 attgggcttg tctataagat tataaaaaaa ttaaaataat aaatagcaaa aattatgtct
     4681 aaaagacagt aaaaatattt tgcataaatt ttgatagtaa tcaataaaaa caattaaagg
     4741 ttttattata ttattatgga atacaaagat tattataaaa tattaggtgt atcaaaaaat
     4801 agtagtacag aagacatcaa aaaagcatat aaaaaactag ctttgcaata tcatccagat
     4861 aaaaatcctg gaaacaaaga agcagaggaa aaatttaaag aaatcaatga ggcttatgaa
     4921 gtattgagtg acccagagaa aagaaagcga tatgatgagt taggtgctaa ttgggataaa
     4981 tataaagatt tcgattttag tagctacaga cctagtgggc gaacctatca ctatactact
     5041 gaggatttag gcgatatttt cggtcaaggt ggtcgtagtc ctttttctga tttctttgat
     5101 atgttttttg gctcagattt taattgggat gatattttta accaaagcta tgaaacacaa
     5161 actagctata gacctcaaga tttcagaact acatctagac aaccacaata tactacagac
     5221 ctcaatataa gtctagaaga agcttaccac ggtactaaaa aaattttaaa tattaataat
     5281 aaacaaataa gagtacccat aaagccaggc atcaaagacg gacaagtatt aagaataaaa
     5341 cgtgacaatc ataaaatacc aaatgtagat ggagatctat taataaatat acacattaat
     5401 aaacatccta aatttgaaag aaaaggcaac gacctttata ctaatctcga actagaccta
     5461 tacactgcat tattaggtgg cgaggtaata gtacctacac tagataaacc attaaaagta
     5521 aaactcaaag aatgtacaca acctggtgaa atcttaagaa ttaaaggcaa gggtatgcca
     5581 ctttatgaaa atcctaatag acatggtgat ttatttatca atataaaagt taatttccct
     5641 aaaaaattga caccagaaca acgaaagcta atacaaaaat taaaaaataa taaataaacg
     5701 agattttttt aaaatatttt aaaatcaatt tttgttattt ttgaaaaaat cttcttttat
     5761 tctccctacc atataaaatc ttttttgata ataaggcatt tcgagagaat ctattctaac
     5821 acccgctttg gtactagcgt gtacaaattg attatcacct atgtatatcc ctacatgaga
     5881 aacgttagaa ttatttattt tgaaaaatac taaatcgcca aatctagctt cctccactgg
     5941 tactttgatg acattttgta tgatatcata ggacgatcta ttgagtttaa aaccatacac
     6001 attttcgtat atagtcatca ccataccgct acaatcagtt ccattttcat ctttaccacc
     6061 atatttatag gggacaccaa tccatctatc tatctctttt atcaaattag tatcagcatc
     6121 actaggtaat tcgatacccc atttattttc gtaaaaacta attactgctt catttaaatt
     6181 ttttgcatca tcagtagttt ttaattcatc ttctttatct ttgcttatag cattttttgt
     6241 tgccatatta gttttagaca cgttatcacc tttgttatgt gtttctatct gcctctgctt
     6301 tacatattct acaataccag tacacgatat aaatgtaaaa gatattaaaa atattattag
     6361 tctttcaccc aaataaaatt ttttcttttt atctttcatg ataatcaaat tcaataaaca
     6421 tataatataa aaaacaaagt acaaatataa atattttttt taattagaaa attaccaata
     6481 atatcagaac cacaggattt caaataatta tcctaattaa tcataaagaa taaatataac
     6541 gaaatacacg ggcaattcac aaattgcaca atgaatgatg ctaagtaagg gtgtatcatt
     6601 ttacaccctt acttagcaat tttataaaaa tatttattat taattttaat caaatatatc
     6661 ccttgattaa atgcttgcaa tggtatgaaa cagtaattac tatttatgtc cttctcatag
     6721 ataatttgtc caataacatt agttataacg acattttcga taatactttc tgattttatt
     6781 atcaaattat tgtctattgt tgtaatatta ataatttcat tagcaaaatc atctatatta
     6841 gtaattgtaa ttgtttgtgt ataggtatcc aaaccacatt cattgcttgc aattagagaa
     6901 acaataaatg aattgctatt attatatatg tgtattggat ttatctcagt actattagtg
     6961 ccatcaccaa agtcccagta aaaataattt gcattttggc aataattaaa aaaacttaca
     7021 atattacctt caatattata ggtgaaattg gcaataggta actcattaac atttatataa
     7081 ataggaatta aaggactctc acacttctct tctatttccc aatcataaaa ataataataa
     7141 taacttgtag cactggtacc agcagtgcta gacgtgatgg aaatgtaatt attaatatta
     7201 aatggataac cgagattagg tccattatca cttccatttc taaataaatt aggataagta
     7261 gatgcagtga ttttcagatt attttcttgt gggatttgcc aatcgagact tatgcgactt
     7321 tcgcccgatg gtatatttat tgtctgagaa taaagtgcat tagaattact atcatacaat
     7381 gtaatagttc tatcagcagt agagttagca tataccttta cagattttag atttacagat
     7441 tgtagacaat caaagtagag accgtgctct acatagttat taaaatatcc accagtacca
     7501 gaattgtcaa gtttgccacc attatatgtg tgactaatca cattatttac ataaatggta
     7561 tcatcattta aaatattgct aatgtaatat tgatttcctg tattaataat tgaattggct
     7621 aaactatcat tataccaata aatattgcca ttcgcagttg cttctatagt aaggttatca
     7681 ccagagcaca cattataatt tgtttgagaa agtacaggac tatcaggcat ctctactgta
     7741 attaaattat tataaataac tgaatctttg cccgcgcagc ttgttacttt taatttaaca
     7801 ttataagtac cattttgagt ataaatatga gttgggttag attcattact agtagtacca
     7861 tcaccgaagt cccacaggaa taagctatta acactagttg tactattatt aataaaattt
     7921 acaatgccag tgcaagtatt tgtcacatct acagtgaaat cagctactgg tggatatgga
     7981 tataaagtaa tatttctcat agctatactt ttgttttcta ttgtaatgct agtaatagta
     8041 tcggattgat aacctggtgc agagactatc atagtccacg agccggcagc aataggtcta
     8101 taataatctc catgaggcaa atgtgaatat acttggctgc tatcagtatc atgattatta
     8161 atagttatta atgctttgat cggttcgtaa gtacatccat ctatgacagt acctctaatg
     8221 ccataaattg cttgttctaa ataatttaaa aaactgcgat aattatatgt ccaaaaattt
     8281 tcaagttcac tagcaggagg agtttttgta tttgaaatct ctaaagtaat ttctctgcaa
     8341 tgagcgaagt aattcatcca atcctgtcga gaaccactta ctagatacca agatgcacct
     8401 tctattacgc ctggatagtc tgctccagaa ggttgatact caaaatatcc actagggcta
     8461 taatattggg ctgtatcagc atattctttg caaatatata tccaccaagt tttatcagca
     8521 tggtcttgag ttttataatc ccaagggtaa ttagctatct cggcaccacc gtgaatattc
     8581 atagccatta caaaaggatg ttttttacca taattcataa aagcttgtgt ctctacctca
     8641 attgagtaag tattgtctcc gcctatacta ccatttggta ctggaaaatt cctatttaaa
     8701 tccatactat tagcattgcc tctagtagcc ccactcacag tattattact gcttgcatat
     8761 gtaccgtcag gattagctaa tggattgatg tgtatctcta aagaatctac tattttgtgt
     8821 attctattat tagtattata attttgcaat aaataatcta tcagatgaag catcaataca
     8881 taacctgtta cttcatcacc atgcattgat gaagtataaa aaactttagg ttctttctca
     8941 ttttgatgca cattatctga aataatagca gataatataa gccttccttc cacgcttgta
     9001 ccaattgtat ctattctaca taaatcaggg taatttagag caaattgttg catcaaagtt
     9061 aaatatgcac tatatgtagg gtagtaatcc caactagtta tttgcttaga taaaatatca
     9121 tcggcatctt ttatatttag ctctgattca ttcaatctca tgctaggggg agtaagtttt
     9181 ataaaattaa gatttagatt attaaagccc tcatactgat taatagtaac atatgcgaaa
     9241 atagtgtcag tggttatttt atcaatatag atagtattac taagtacatc aatatcaatg
     9301 ttattatttt tgtttggaat agctatgtat atttcttttc taccatcaaa aatagaataa
     9361 ttagtagtag tttgaccaaa taataaattg gtttctatga taaataataa attaattata
     9421 aaacaaaata attttttcat aattttaata ttttacaaat gggattattt ttatacttcc
     9481 atctcgcaca gtaatatggg agtaatatat tccattagcg atgttactta aataaatgtt
     9541 tttaacactg ttagcctcga ttatttcatt ttctacaata cgcataccaa ggggattata
     9601 tatagagata gaaatttgat cattagattt attattaaat attaaagctt gataatcgcc
     9661 agaccagtat agtgaaaaat cattgtcata atcacttata gcttcattta tatgacaggt
     9721 aatattgata tcatcagtgt gagtacaacc acagtaatca gtagcagtta aagagtaatg
     9781 ataattttgt ccattttgag gtattaatgt tatagtttgt ccaatagatt catcagacca
     9841 gtgatatgaa gcaaattcat caccagcatc aatagtgatt tgtcgtccac atatagtatc
     9901 catatctggg cctaaatcga gagtgagatt aggatcgcat tgacctttta ttaaaagact
     9961 atcaatatac caattatcac cattattttg ggccatcaca aaagctatat aaacattttg
    10021 atcagcatat gcactcaaat caatagtttt tttagtatat gatgtgctaa aactagactc
    10081 attccactca gaaacagtag taaatgaaga atgtgaagtc tgagaagtag tagaaacacg
    10141 tacactataa gtagatccgt agttagtaga aaaatcttgt ttttgataaa aagaaagtac
    10201 tggatcatca cagagatgta ttttaggggt tactaaccag tcctcagttt gtccaccata
    10261 gactatttca taacgaacaa aagctgcacc ataaccctca taagcttcat tagtactttg
    10321 ccaattatag ctagtaccta gtccattagt accaatataa actgcccagc aagctggcgg
    10381 gaaactacta ttaaacaatt ctacaaaagg agaaggacgt aaagatggat aaacagttcc
    10441 ttgccaattt aaagtatcat tatttagatt ttcgtctaca cctccattag gattggcaca
    10501 ccatactttc agattatgta taccttcttc taataaatat gaagctaaat ctacagttgt
    10561 tgtttggtta gtagctagtg agcccgtcca atttattaaa tttgtagagc catcatcaaa
    10621 ggtccaataa attttcaaag aagttaaagt cagattacct ctatttttga taacagcttg
    10681 gaaattttgt gtagaaattt cacaaaattc attattacta ggcaaaattt gaataacaga
    10741 agcatctaaa tcagcaacag gagtattagg atcatagcct tctaatattg taacactact
    10801 attagtagga tctaaccaat cttttaatcg attactagag gctccaccac catcccaaga
    10861 tacaccaagt ttaccatacc aatctgaact agtatttcca caagctgcat atcctccatg
    10921 taactgacct ataatatgtc cagtaggatc aaaaatagga gaaccactag agcccggttc
    10981 agtagtcgta ccatcactcc aggaagttac cctccagtgt gttccatcac ctggaataga
    11041 attatttaaa taagttgtag tactcacgcc attattagac caactaattt ttttaatatc
    11101 acctgaggga tgatgaatac ccactacttt gccactaata ttattatcca tagttctatt
    11161 ccatccagag aaatatacat catatgtcga aggtatatta ttatttattt gtaccaagca
    11221 aaaatctgaa ttagcatatc tagctttcag tgtagctcca ctcatagaat tataaggagg
    11281 agaactaact ggattagtac atgtaggact ttgccaatta aaccaaaata cccagctcga
    11341 tggatcagag taacagtgat ttgcagttaa gacataaggg gtcccatcat tatttgtatt
    11401 atttatcaaa gcaccagaac aaaaaccatc accacccgat accaacatgc ataccgatcg
    11461 tatctgatct tcccatcccg cagcctgtgg gcatgctaca tttacattac aactacctgc
    11521 atttccaaat gatttcaaag catattcata agcatttcta taaccatgcg ttattctata
    11581 cagatgtaag gtaggcagat tgttaattgc agcatcttgg tttacttcga ttataacttc
    11641 atctccagga attaatgttg tagcgaaaac accatcttcg ctattattgt tatgattaaa
    11701 agctcctaat accatgcttt tatcagcatt atataaatat aattcagtat tttctggtaa
    11761 ataaaattga tcaaaagtga aatttaatgt aatggctcca tctgacttaa atgtcattaa
    11821 ccatattttt ttgccattta tgattgtatc aaaagcttga tccataatat ttatatctac
    11881 atatacattt tgaccaaatc tccaaggtct atctttgtat tggtctacta tagcatcctc
    11941 cattttcagt acttctacat cgatatgagg taaaatgtag atattagttt ttttaatatt
    12001 atgatcaaaa ctagcaggga tacctggata acttatttga cagtaagaaa ttaaactgac
    12061 taaaaataag attaaaaata atattttttt catagtgcaa ataattttta taaaattata
    12121 ctatttttat aaaataaaaa atttttttta ttaaaaaaat ttatgtattt ttgcaaacac
    12181 aatagggccc atagctcagc aggttagagc aactgactca taatcagtag gtcccaggtt
    12241 caagtcctgg tgggcccatc ttttttttaa tattccttat taaagtatat ttatagcttt
    12301 agactaaatt gaacatttaa tatatcgtaa aatctaataa tttttttcag taatgtttaa
    12361 tgttattgaa aaaaaatact tttataatat ttctaaagaa ggttctataa cgttttatat
    12421 gggtaaagat ttaaaaaaaa actaatttta caaaaataaa tttgttaata ttggtttgtt
    12481 tattattgga cagttaaata ttaaccacaa agttctcaaa gaaggcacca agaacacaaa
    12541 gaaagatgga attaaaaggg ttataaatgg cactttgtga actttgtgta ataacttggt
    12601 gccctttgtg gtaaaaaaat tagcctaaat aattaaaaaa tggttatttt ttgtaataat
    12661 ctaaaatgtt tttaccaaca ataaacgtta tagaacccta aagaataaat aatcttgctt
    12721 aagttaccta taattaatag atttttcatt aattatataa aatcgaagta aaatacctct
    12781 cagtgtagat caataatggt acttatagat attgattaca atttaatcta ggtccattaa
    12841 taagaatcta tatcaatcta tgtattctga cactgagtgc taagatatta tatagtatta
    12901 aaaaaagaaa atgtatgatc ttttgacacg tggcgatatc gatgtcatca catgatatgg
    12961 tatagtttta ttaactttag catagtctaa aattgatata tcatcgccaa aaattattac
    13021 tttgtcacct tcatttatat ttggccagat actagttaca tctaccatca tcatatccat
    13081 gcatatatca cctactacag gagcatattt atcatttatt tttacaaagc cgtttccatt
    13141 ccccatttct ctatatatac catctgcata accaattggg atagtagcta tcactctcga
    13201 atgctcagat atagctttac gattataacc tatagattct ctagcattta tctctctaat
    13261 ctgagtaacg aaactagata attctgctgc tggcatgagt tttttattta tttcatctat
    13321 attagtaata ccatatagtc ccaaacctat tctcacataa tcccaatgat attcaggaaa
    13381 tctaatagta ccgtgtgtat tacaaatatg tttttttatt ttataagaca aatcattctg
    13441 aagcttttca gtaaagtcat ttaatttttt agcttgtagt tttgtgaaat catcatgttc
    13501 gggcatatca gatgcagaaa aatgagaaaa aacagtagat acttttatta aatctttcac
    13561 ttcttgcaac cttttagata acatagtata ttgttcaggc aaaaaaccta atctgtgcat
    13621 acccgtgtct atttttagat gaatgggata aggtataggt gctagaaaag aatattcctt
    13681 tacgacttgg atcatttgtt cgagagctaa aaagctaaaa atttctggtt ctaaattata
    13741 tttaatgcac atctgcaatg aagataaatg tggactcatt actattatcg gagatttaat
    13801 accagatctt ctaagagata ccccttcatg agaaaaagca acacctaaat aatcaacacc
    13861 taattgttct aaaaatgatg aaatatcttc ggctcctgaa ccataagcaa atgcttttac
    13921 catagctatg atcccagtat ctttagagat tattgaatga aaatactgat aattgtgctg
    13981 aatagcgttt aaagatactc ttagctctgc ataagaattg ttttctacta aaaatggaag
    14041 taatttttca aaagtatatt ttctggctcc ttttaataat attaagctgt ctttgaattg
    14101 atctggttct attttcatta cacactcttc tgtagatctg aaatgacctt ttactctgtg
    14161 aaaataactt gataatttta ttatattatc cccaacagta tatacctcat cgactttgtt
    14221 tttatccaat tccttagcta ttttttcgta taggacctct atactttcat cactttcgag
    14281 aatgtcagac aaaaaaacaa ttttcttttt gctctctgat ttttgattta aatattgtaa
    14341 tgagatgcta agagacaaaa ggtcattaga gtagaaatca ctcactatca aattgccatt
    14401 gttaccatgc acagtttcta acctcataga tatagtttcc cattgtggta tataattttg
    14461 tatttcatta gccatcaact cattagttaa acagtaggta atgacatgca tagcattttc
    14521 tatagaagct gtatcgataa atggcagaga aaaattgtaa gttttatcat catagattag
    14581 ctctatctcc gtcacccatt tatttgtaga cttatttata atttttatgt tatcggtaga
    14641 ttttgtaccc caagttatta aatttataga taatgaacga gcttctttga ttatttgatc
    14701 ataatctgag caaacaatta atttttttgc gttgattgct aattttaatt tttctttaat
    14761 tttttcagaa atatttttaa aatattgctg atgggcaggt ccgatattag taaaaattac
    14821 agtgtcaggt ttaatgatat ctgctaattt atccatctca tgaggttgtg aaataccaac
    14881 ctcgaaaata cctatttgat gtttagactc taattgtaaa atagaatatg gtagtcctat
    14941 ctgactatta taactacgtg gatttttagc cacttcaaat ttattagcta atgatatagc
    15001 taaccactct tttacaatag ttttgccatt agatcctgta atagcaacta tagggatatt
    15061 aaattgttta cgatgatatt tagcccaatt ttgtaatgca tctaaagtat tatcaactat
    15121 aaaaaaatta gcattttcat attttttaat atattttttg tctttaatac aaaaatttct
    15181 tatcccttta ttataagcat catctataaa caagtgccca tcttgtaaac cctttagagc
    15241 aaagaaaata gcagtagagg gcttattaat aggcaatcga gtatcgaaga caatctcatc
    15301 tataaaaaca ttttgaatat catttttttg tgaaaaaaaa actgcattta tgatattagc
    15361 taactcaatt gctcgatact gtctcatctt ttaattgtgc ttttatattt tcgtcataat
    15421 atctgcgtct caaagtgatc tcagcaccca gaagtattgc ttgtacaaaa gatctttcat
    15481 tagctttatc ttttccagca atgtcaaatg cagtgccatg cacaggtgag gttctgacta
    15541 taggtaaacc agcagtgaaa ttgacagctt cttgattatc taataattta aaaggtatca
    15601 agccctgatc atgatacata gaaagtacag catcgaaatc tttataacgc aaattaccaa
    15661 aaaatgaatc tgctgggaaa ggtccataag cttctatacc tatctcatta gcttctctaa
    15721 tggcaggtat tattacatca ttttcctcat tgcctatcaa gccttcatca ctggcatgtg
    15781 gatttaatcc taacactgct attttaggct taataaatcc aaaatccctt tgtagagaat
    15841 tgtccaagac ttttatcttg cttaaaatca aatgtgcatt taaaatattt tttaattcag
    15901 aaataggggc atgagtagtc actaagccta ttttaagata aggatttata aacatcatta
    15961 aatattcttg tatattaaaa gcatcagcta aaaattctgt atgacctttg aaagggaaat
    16021 ctattaagga taatgctttt ttatttatag gacatgttac caaaaaatct attttgccct
    16081 cattaaacca tttaatacct tgctctaaag actctatcga tgcttcggct cccacctttg
    16141 ttatttttcc tatctctatt ttgtattcaa tagaactcac tgggtagata tagggcttgc
    16201 caggttttaa atttttcaaa ttttttagaa ggaatagatt tacattacag ttgttaatca
    16261 tttttctatg atatgcggct gcacgtggat ttcctaaaac tattggtgta catatatcaa
    16321 ataattgtat gtttttaaaa gctttatata tgagctcgta tccaatacca tttagatcgc
    16381 cgggagtaat tcctgcaatt aaaaaatctc tatctacatt attcattttt tttacaaatt
    16441 tacaattttt cttttttatg ttcatatcga ttaatataca attttctgat taattattat
    16501 taaatacagt tttttttaaa aaataattgc taaccatatt aaatgtaata aataaattgc
    16561 atatttgtag ataattgatt atacttttat agataaaaat ttaaaattgt atcatttctg
    16621 aattattttg atttagtcta atttattgat ttgttttgca aaatcaattt gatttttatc
    16681 tttattagta attttgtaat ataaaattta ttaagaattt atttatgatt aataaagaga
    16741 caattagtga atggagagat aatgttatag aaaacctaaa ttttgcaact tttgattttt
    16801 tattaaaaga tatagaaaaa gaaaatccta ctttcactcc aaaatttgta aaaacagcta
    16861 ttaaaaatat attttatgca ctcacaccag aaaaatatta taactggata accaagtata
    16921 atattcctaa taaaacacat agtctcggaa taatttgtgc tggcaatgtc cctgctgtca
    16981 gcttttttga tgtttttatg ggattagtaa ctaatcatga agtttatgtg aagctatctc
    17041 attcagatac taagctaata ccttatttat ttgaacaatt atttaacttt attccagaga
    17101 tgcgagatag gattaaattt gttgaaagta ttcattccgt aaaggctgaa aagataatag
    17161 ctacaggtag caataatgct aataggtact ttttacaata tttcgctgat aaaaaagcag
    17221 tattgagaca tgatcgtaaa agcatagcag tgataaatca tgatattact gaaaatgaat
    17281 tgaatgcttt agctaatgat atttttcttt atttcggttt aggttgcaga aatgtatcac
    17341 ttatactttt gccacatgaa ttttcgctaa acaaaatcaa tgcagcattc aaacactatt
    17401 cgtggatttc ggataatctt tattataaag agataataaa ctactataga gcatattttt
    17461 ctttaatgtt tgaagatttt attgataatg atttttatct attacgagaa aggttggatc
    17521 tgtactctcc tatttctgtt atcaattatg cttattataa tgatatatcc caagtagaaa
    17581 attttataaa agctaataaa gaaaatttac agtgcattgt aggaaattat tcattatgca
    17641 atacaacatt tggtaaaacg caaaaaccag aattatcaga ttatcctgat gacgtagata
    17701 taatgaggtt tttgacggaa tcttgagatt tttatacaca aaatcaaatg ttttgaaaaa
    17761 aaacataata attaaaattc taaatactaa agtactttac tctacgtaat attttaatat
    17821 atacagcctt gatattaatt ttattattta atagaataaa caaagcaaaa atcacaattt
    17881 tctaaacccc tcgaaacttc tcatcttttc tcatgctccc cgtctacatt gtattttttt
    17941 ttattaggtt gcaattttaa aaaaattaaa gggattcaaa atttgaaccc ctttaatttt
    18001 ttgaaatttt atcactataa actacaagtt aatttataat agttaattcg tttaatatat
    18061 tagtagcacc agcatattta tcaataataa ataatacgta tctaatatct acattaatgc
    18121 atctctgtac acttgtttcg aatagaatat caccactcat actttcccaa ttaccatcaa
    18181 aagcgaggcc tattaagtgt ccattagcat ctataactgg gctgccacta ttgcccccag
    18241 taatatcatt attagtaatg aaacaagtaa tgatattgcc atttttatca acatagggcc
    18301 caaaatcctt agatttatat aattctttta atttatctgg aacaataaat tcctcattag
    18361 taggatcttc tttttccatt acacctttta atgtagtgat aaaatcataa tgtactgcat
    18421 ctgcggggaa ataatcttgt actgtgccat aagtcattct catagtgcta ttagcatctg
    18481 gataaaaagc tttttctggc atcatttctc tcaatccagc tatgtatttg cgataacctt
    18541 ctttttgttt ggcttcagct ctagacatct cttgccatat ttcacgtaaa ctagataaag
    18601 tattaagtgt gatttggaac cctaagtcgt tttctagggt ttttaattta ggattttgta
    18661 aaaagttatc caatcttgtc tcatctacta atatactttt acgatagata tcatctgccc
    18721 accttttgat atcaccttta tatttttttt ctaaagtttt gatagctgat atttgttgtt
    18781 cctttggtac ttcacttgtg tacatttcaa acatagctat gaatatatct ttatctacat
    18841 tgacattata atctttgaaa tgttttttgg ctttttcaga taaatcttta gctattaatt
    18901 ttatatttgc ttctatttta ggcttatcat ttttatcaac attatttaga tttttcaata
    18961 aagcatataa ttgtaaaaaa tcaaaaccaa aataaataaa ttcaggacct tgtagtatgg
    19021 cttcttctag ataagttatg gttttattat aattttgtat taattcataa gattctttaa
    19081 tctcttgcaa gcaagtacca tatttttgtt gtctattttg atcttgttgt acccaattaa
    19141 taaattgatt ttctaattct ttttttctat tataaacatc ccatcttttt agtcctttag
    19201 tttgtccaat ataatatttc cagtagttag atatctgagc atatttagaa gcgtattgta
    19261 atttgatctt aggatctttg ttcatataag atcgcattat ttctaatttt ttatctctaa
    19321 tattaactat caaaggatta gattcattaa tagctatatt cacgccatct gatgtcagat
    19381 atctattaga gcttccggga aatccaaaga tcattgcaaa atcatctttt tctacacctt
    19441 gattagaaac tggtaaatat tttttaggtt tcaatggtac attatttttt gaaaattctg
    19501 caggtgaccc atctggagct gtataaatac ggaaaataga aaaatctcct gtatgtctag
    19561 gccacatcca gttatctgta tcaccaccaa atttaccaat agcatttggt ggagtaccta
    19621 caagtctaat atctttatat gtttcataaa catataaata atattcattt ccttcaaaca
    19681 taggtttcac taccgcagta tatttgcctt tttctgaggc ttcattttct attccttttt
    19741 tagccttatt tatagctttt tctctagaac tatagctcat agaatcgtct atatctctta
    19801 atattcttgc tgtaacatct tcgattttta caacaaatga catagtcatc ccttcgattg
    19861 gtatctcttc ctctagtttg ctagcccaaa aaccatgtgt taggtaatca ttttctaacg
    19921 tgctcacttt ttgtattgca tcatatccgc agtgatgatt tgtaaatacc atcccttgtg
    19981 gtgatactac tactcctgaa cagaaaaaac caagatttgg agtataagta ctggtaccta
    20041 atcccacaat agcgtctttg atacttggtt gagtattcga gtatacttct tcggcactca
    20101 gtttgatacc taatcttgcc atttctggat aatttaattc ttctatccat actggtatcc
    20161 acataccctc gtctgggcgt gtgggaggtt tcaagccaaa tgatgcttgt gttacaaaca
    20221 ataataacaa taacgaggca aaaaatcttt tcataattca attggttttt tacaaaagta
    20281 ataaatatta tctttgtgta tagctattaa aagctttttt agataaaatt gacatcaata
    20341 ttaatgagta atatgatatt gtagtttaat tatcattatt tttatgtaac gtaatattat
    20401 aacaaaagtt atatttaata aaaaatattt tttattaaat tatttatgca acgtaatatg
    20461 tggaaaatat taatatatac cggaataata tttataataa taggtataat atttttttta
    20521 tttgaaaaat ttcatttcaa attacctggt gatattataa ttaaaaaaga aaattttagt
    20581 atttatttcc caatagttac ttgtatagtt ttaagtataa tattaacttt attattaaat
    20641 atttttaaga aataaagttt tataactaat tacgtacaaa ttttatcaaa tatacttcta
    20701 tacttcttta atatatgctg caaattcaca tatatatttc ttaatatttt taaaaaaaag
    20761 cagtaataat aaagtatcaa tcagagatta aaatacttag tagttttgtg taatcctttt
    20821 taattctata tgaaagaatc attcagttat tatcaatagc aaaatcaatc attttgatat
    20881 tcagataaaa tctttttaac atcatctgga gccactctac cgtatgtttt ttctcctacc
    20941 atcactacag gagccagacc acaggcaccc acacaacgca agcaattaag agaaaattta
    21001 ccatctggag tactttcgcc gactttaata tttagttgtt ttctgaattc atctaaaatt
    21061 ttttcagcac cacggacata acaggctgta cctaaacaaa tgtttattgg atattcccct
    21121 tttggtatca tagtaaaata agaataaaag gtaacaattc catatacctt agctgtaggt
    21181 attttcaatt cactagctac ggcttcttgt acttcactgg gtagataacc aaattgatct
    21241 tgtaatttat gcaaaatatt aattagctca ccaggatcat aattaaattc tttacaagtg
    21301 tctttaatga aatttaattg gctttcgtgt aattttattt tgatatgaga cataatacct
    21361 ccttggattt tttaatcatt ttcaataata atttcaggtt gttttttgaa atattgtgta
    21421 tgaagcaatt catgagacag ttcactcata ggatgtccga gatattcttc atataatttt
    21481 ataatatatg gattttcatg agattttctt aaaggcaatc ttctatcttc ttcgtaaata
    21541 gctttttgtc tagcttttat aatttcactt ttcccatgat gttgtggttg accaccaccg
    21601 ccaatacaac cccctggaca agccattatt tctattgcat gaaattcact ttttccagct
    21661 tgtatttctt ctaaaagttt acgagcattg cctaaaccat gagcaatacc aatgttaagt
    21721 ttgagaccat taaaatcaat agttgctttt ctgatccatt ccataccacg tagttcttcg
    21781 aattctattt taggtaaagg tttcttagtg tagagttcat aagctgttct cacagctgct
    21841 tctattacac cacctgtagt accaaaaata gcagcagcac ctgtagattc acctaatggt
    21901 ttatcaaaat cactctctgg caaactctca aaatcttgta cagtttgttt tattaatgta
    21961 gcaagttcac gagtagtaat gctaatatta acatcaggat catcattgac tctaaattct
    22021 tctctttgag cttcatattt tttggctacg cagggcatta cagatactac aactaatttt
    22081 tcgcgcggta tattgagttg tttggcaaaa taatttttag ctatagcacc aaacatttgt
    22141 tgtggagatt tagcagtact tggtacatct aataaatcgg gaaaattttt ctcaaagaaa
    22201 ttaacccatg ctgggcaaca agaagtcaaa ataggtaatt caactgattt atcgccattt
    22261 aaatatcttg ttagacggtc caataattca gtaccttcct ccataatagt tagatcagca
    22321 gcgaagtcag tatcgaatac atagtcaaat ccaattcttt tgagagctgt aaccatctta
    22381 ccagtaacaa tcgtacccgg ttttatacca aattcttcac ctaaagttac tctaacagca
    22441 ggtgcagttt gtactactac gactttatca ggatctgcta aagcttgtat gagtttacct
    22501 gtttgatcta cttctgttaa agcagcagta ggacatacag ctacacattg cccgcaataa
    22561 gtacaaggac tatgatctag attttgttca aaagctggcg ctactactgc cgtgaaacct
    22621 ctattgacag cagataagac accacaagtc tgaaccgtat tgcacattaa ttcacatcta
    22681 cgacacatta tacatttatc cggatttcta atgatagctt gagatgtatc cattctatag
    22741 gtagattgtt cacctgtata tctaatttct ctaacaccaa gctcttgagc taatgattgc
    22801 aaatcacatt ttccattttt agaacaaatg agacattcaa aaggatgatc gcttagtatt
    22861 agttctagaa cagttctacg agcattaatg attcgcggat tactagtttc tattaccatt
    22921 ccttctgtac attcagtaac acatgaagga gctaaatttt tacgaccttt tatttctact
    22981 acacaaattc tacatccagc tggtttattt tctatatcga aatcttccaa tttcatatga
    23041 cataaagtag gaatttctat atttatcatc ctagcagctt ctaaaacggt agtattttta
    23101 ggtacttcta atggtatgtt attaattgtt atatgaacag tttccatatt tgtatccccc
    23161 tttttttttt taaataatat atactgcatt aaatttacat gtttctaaac aaataccaca
    23221 tttggtacac aaatcttgat tgatcacatg aacttgttta cgctcacctg ctatagcatt
    23281 tacaggacat ttgcgagcac aggccataca tcctgtacaa ttttcgggat caataaaata
    23341 atgaattaaa tctttacatt tattagctct acatttttta tcagttatat gctctacata
    23401 ctcatcccag aaattattta aagtagattg aactggattg ggagatgttt gacctaaacc
    23461 acatagagcg gtatctttta taaccataca aagatttttt aatagttcga gatcttccat
    23521 agtaccattg cccttagtaa ttttatctaa tatttcactt aatcttttat taccaattct
    23581 acatggatta catttgccgc aactttcttc tacagtaaag tctaggaaaa atttagcaac
    23641 agacaccatg caatcatctt catccattac tatcatacca cctgaaccca tcatagaacc
    23701 agcagctact aaggtctcat attctatcgg agtatctaga tgtttttctg ttaagcaacc
    23761 accagacgga cctcctgttt gaacagcttt aaatttttta ttatttttga taccaccacc
    23821 aatctcatag ataatttcgc gtaaagtaat gcccataggt acttctatta atccaacatt
    23881 atttatttta cctgcgagag caaatacttt agtgccttta cttttttcag aaccgataga
    23941 tgtaaaccaa tctggtcctt ttaaaattat aggaggcaca ttagcaaaag tttctacatt
    24001 attaacattg gtaggtttgc ctctataacc tgctacagct ggataagggg gttttacagt
    24061 tggttcccct ctattacctt ccatagaatg aattagagca gtttcttcac cgcatacaaa
    24121 tgcacctgca ccatagcgta actcaatatc aaaggagaaa tctgtattga aaatattctt
    24181 tcctagtagg ccatattctt tagcttgttc tattgcaatt tgaagtctct taatagcaat
    24241 tggatattca gctctaatat atattagacc atgagaagca ccgatagcat agccacaaat
    24301 agccatagct tctagtacac tatgaggatc accctctaat atgcttctgt ccataaatgc
    24361 tccagggtcg ccctcatctg cattacaaac aacatatttt tctgggccag gattagatgc
    24421 ggttatttcc cattttacgc cagtaggaaa accagcacca cctctacctc ttaagccaga
    24481 ttttttgata atgtctatta cttgtgatgg agtaaactca tttaaacatt tatataaagc
    24541 taggtatcca tcccttgcaa tgtattcttc tatattctct ggatcgatga caccacaatt
    24601 acgcaagact acacgtagtt gtttttgata gaaattaata tgtttagaat cagtaacagg
    24661 ttctttagta actggatctc taaagagtaa tctttctact ctacgacctt tgatgatatg
    24721 ttcatttata atttctttag catcttcagg ttttacataa ttataaaaag tgttatcagg
    24781 taaaactttt actataggac ccctttcgca aaatccgaaa catccagtag taacaacctt
    24841 tacaaaatct tcgagttggt tttctttaat ttcttcattg aggttttgta ctatttgagc
    24901 actttgagat gatttacatc ctgtacctcc acacacaaga atgtgatatt taaatgttcc
    24961 catttttatt ctcctttatt tatcatctat tgttttataa ttttgaggga ttatgccttc
    25021 gattagttct ccattacgga tatacttttc tattatttca tcagctcttt ctttagtaac
    25081 ccatccaaag acaataggtt cttgtccagg aagttttact tctacagtag gttctgcgta
    25141 gctataaccc atatctccag tttgagttac tacggcagga atatttcgct tttctaattc
    25201 atctacaaaa aacgtaaaaa tttcctttgc tcctgctgca ataccacttg tagccatacc
    25261 tactttgact tgtactaatg attcagggtg ttctgctttt tccctgagat taatttggtc
    25321 ctcctgattt ttttttatct ttcggagatc atctagtgaa gtaatttttg ccataatatt
    25381 ttatatttat tttttatatt taacattgcc tcttagtttt atgtttaata aaatttggca
    25441 attcatttct taattttttt gcaaagttaa tatttatttg ttaatgtaat aacattgtta
    25501 tataattaac tatatataaa aatatcataa atttaattga tttaaatcaa ttaaaaaatt
    25561 tattttttac aaatttatta tttcatagct tttatttgct tcaaattttc atctatatat
    25621 tcttttaaac tccttctcat tataccattg gtacatatgt cgttaataat atctttaata
    25681 tcgagggtat ccaatacgaa ataaccaaaa tcagttttgt gtttatatac aaagtgtata
    25741 ttaggatgcg ccataataag taaaattatt gttcctgcta tatctcctag aggttgtcta
    25801 tctacgtgtg ataatttaaa agatgctatt acttctgttc ctttattcaa ttcacttttt
    25861 atctgaaatt taccttcaga caattcagca tgatatttta aaagtgatat acctaaacca
    25921 acttttttat cttttttagt tgtaaaaaat ggatctgtta ttatttttat agtatccgta
    25981 tctatacctt taccatcatc gataattgtt agtttcaaat aatcattttt aatactttct
    26041 tctatttgaa tagtaatatt ttgtgctcca gcactaatag aattatttac aatgtctatg
    26101 atgtgatcaa ctatttcttt catatgttat cgttataatt aatgcaaaca tctctaccat
    26161 caattttgta aagcgatttt ttgatttcat gaaatgaacg attttttata tgaaaaatcg
    26221 ttgtaataca accaatatca ttaataaaat gagcatcaga atctgatata aatgtatttt
    26281 tttttgtata aggaaaagaa ttaattatat tttgcggatt agaatattta gatacctcaa
    26341 aagcatctac atttagatct tttggcacta tacctaattg actaatcaag ctataagcag
    26401 atctatctat atgtgctggt ataaaaaggc cattgagttg gtgaactttt ttttctaaat
    26461 catcaataga aatattagta gcattaatca gtaaccaatc tatttggtct acaatatttt
    26521 catctttatc tacaatcaat tgatatccaa atttatcagg gtcattatca attttaataa
    26581 tattatcatc gataaatttt tgaaataaat ctaaagtctc aaaatcctga aaaaaagtta
    26641 agcaatgagt ttcctctttt gtagtaactt ctaaaccgca aagtgtaaaa ataccataat
    26701 cttttgcaat atcaaaagta actttgcaat tggcagaagc attgtgatca gtaataccaa
    26761 taatatctat tcctttttta gaagcttctt taacaatatt aataggactc atgtctaagc
    26821 taccgcaagg acttagtact gtatgaatat gtagatctgc aataaaatcg tacaaaagat
    26881 cacacatcat cattaaagtg taatgctaca tataatttac cagcagtatc aaaagatgac
    26941 atagaagtag agatgatagg aatattttct tgttttgcag cttcgattag atcatcggct
    27001 ggtgtattat tattagtaat aatgatgcat gataattctt taagtgacgc gatagcaaca
    27061 agatttttat gagtttgtat agttatccat gccatatttt cattagcatt ggccattaca
    27121 tcactcaaaa gatcagatac ataaccatcc gttatctgat tatttaaaga attttcgcag
    27181 catacaattt tggtattttc ccattttaat atatcagcta ccttcatatt tggctccttt
    27241 ttttgaacaa tctttatcga atacattttc gccccaaatt ttagctagaa tcttttttgc
    27301 ttgctcggga tcagttaagt ttttatattg aataaaaaca caatcagata tatgagctac
    27361 gttattagta atatcttctg ctaaggtttt gcaattaggc gccccacata atccacaatc
    27421 tacattaggc aaaaaacaca ttatatctct gatgcgttct aattttttga tagctttttc
    27481 taaatcatta tccagattca ttgggcgagg ttctatatta gttatactaa ttttatctat
    27541 taataacttt tcaatctctt tgtttaattt aatatttttc ttttttttaa tagattgcga
    27601 agcacgttta tataaatttt ctactgtaag aaatctattt ttagtgcaaa gaattccccc
    27661 agcgcaactt tcatcacaag ctcttaattc taaaaaatct acatcttgaa tttcttcatt
    27721 ttcgattttt tctaaaaaat cacatacatt taatatgcca tctaccgcaa aagatcttcc
    27781 tttgacatat tgagcttcgc cacgcgtcaa actccaagta atagccatag gaagcaattt
    27841 agatggttgt atatctaata gttccaaatc tgaatactgc ttgcttttac atttattcat
    27901 aatcatatta aaaaaactat tcatattaat tactccatta ataacacttt tatcttcacc
    27961 aactggactt ttgatagcag ctatcttagc agcacatgga gtaatataaa aaataccaat
    28021 atcatctgca ctaaaacctt gattagtaaa gttatctaat attatctcag cagttaagtc
    28081 tttaggagta cgcaatttga ttatattatc agtcaatgaa ggaaaacgaa cttgaataag
    28141 tctgacaata gctggacaaa atgaggatat caatggttta atattagatt ttttttgata
    28201 atttataatt tgttttctca aaaaatctat agaatgctct acttcatata tatgagtaaa
    28261 gccaagttca taaattgatt tataaatttg cttaatagat atgttgtcag gaaactgccc
    28321 aaacattact gatggcacta gagctacttt gactttatat ttatctaaag cagacaagtc
    28381 atcttcttta attttaatag cacctatagg gcaagctcta aagcattcac cacaatcaat
    28441 acatctatcg ggatataata ctgctttacc atttcttact cgtaaagcgt gagtaggaca
    28501 aactctcatg caatgagtac atcctacgca taaagatcta tcaatttcta aagcatgaga
    28561 aaatatttga ttttttgaca tattttaaaa ataatttatt aaaataactt ctgtaccttc
    28621 attaacgtga ctactgatat tcatataatc agtattcttt ttcatattag gcaatcccat
    28681 acctgcgccg aagcctaatt cacgtacgat aggtgaggcc gtagaatagc cttgttccat
    28741 ggctttattt atatctggta tgcctggacc ttgatctttt aatgttatct tgattttttc
    28801 agtatcaatc tccacattta tcacaccatg atatgcatga gctacaatat ttacctcgcc
    28861 ttcatataaa gcaataacaa cttttttaat aattttagga tctatattca actgttttaa
    28921 tacttttttt acttcgctac tacatttgcc tgcattacta aaatcgccac cttgtacttt
    28981 atatgttaaa atcatattta aatcaataaa ctggtttgac accaagatta tataaaatac
    29041 ctgaaacttt aaaaacagaa tacggacttt gtattacaag tattttattt tcattagcta
    29101 atttaataat atcattatta atcattttat ttctagcata aatcacatac tttatttcag
    29161 caagttcagc tgttctaata gtttgaatgt tgtttaaacc agttattaat attacgtctt
    29221 ctttatcaat tgtcaataca tcactcatta gatctgatga aaatccatat tctacttcat
    29281 catctagata ttctttacat gtataaactg taccgtcgac tgcttcaact attttactca
    29341 atttaactcc cattatttct tataattttt aaagattaaa ttaattagtt ttactttaat
    29401 ttttcagtgc aaagttaata tttatttgtt aattaaataa caatgttaat taaataacta
    29461 taataaaatt atcaaataaa ttattgatat taatcaagta aatcattgat atatatttat
    29521 ttgaaaaaat attttaacta ttaattactt acaaaatatg taataggttg aaaaaatgaa
    29581 gctactacat aatattgaca agttactaat ccaagctcct cattatatcc atgcaataaa
    29641 tctctatctt cataaataat cctaccaaaa gaatcttcta ttccatcagg gtaatatatt
    29701 ttgtaaaaag tcatttcctg cgttgcatta ttattactta tcaacctaat atgacaaaaa
    29761 ggctgttgat gtaatataga gtccctttta gctgagtcta aataagataa catctctgca
    29821 aatctaatat taacaaaacc actcatataa ctacgtagtt taggtaagtc tacattattt
    29881 atttcttgat tattataatc gtacaagtga atattatcat tattttcctt aataatcgta
    29941 aaggaatttt caggcttatc gaaataatca acctctactt tatgaatttg ctgataatac
    30001 atattcataa aagtatgatc tctccaatca gcttctatag gagtgaaata attttgtaaa
    30061 aaacccatca ttccaggaat atacataata taaggttctt tagcaccttc cattaaaaaa
    30121 tatgtaccaa ttttgtctgg tggattgtcg cctacataaa atgtacgtat ttttcgttct
    30181 ttgggaaaaa tccatagcga acctatacga gcccaaggta ctcgtgcata tatttctact
    30241 ttaacaccaa aactagacat acgtttcaat acattatctc gagaagtaat aggaacagga
    30301 cttttgagag aaacataata tagagtcctc ataaactcat ttatcatatt attattagct
    30361 tttaaactat catttactaa ccacccttca ctggttcgag ttaaaacgac agaatttcct
    30421 tttttatcag ctaaaaatat tttagttata gcactcgtgt cggtaatgtt aaatgcattg
    30481 tctctcgggt ataatgcagg atctgtccga tgtagaataa ataatcctac tatcacaatt
    30541 actataattg caaggataat tatcaaaagc gtctttttag tttttgcttt cataaaaaat
    30601 tttttaattt tattaattgc aaaaataaaa aattaattac aaattcaata atacctatta
    30661 atttttaatt tatgaaattg cacttcaagt agcttttttt agcaaccttt ttgttatcca
    30721 acctcttctc acccgcattg ggaaatgatt tgccataaaa caagcaagca gggattggaa
    30781 gaagggttta cactgtttga gcgagcgata gcgagcgagt ttgtaaaccc caagaggtga
    30841 ttgcgtagtt ttatcaaatc atttccccag cggagacttt tcttttgtta ccttttcttt
    30901 gtgtggctga caaagaaaag taaattaagt agaaatttat tccaaaatat tcattaattt
    30961 tacctaacaa aatttataaa tgaaaatcca ctaccactcc gaccatctcg gctccgcctc
    31021 tttcgtaact gatataggtg gtaatgctgt gcagcatttg cagtatctcc catacggcga
    31081 gctattcgtc tcccagcgaa aatctaaaga gtttgatagt aggtataaat tcaccgccaa
    31141 ggcgctagac aacgaaacca gctacacata cttcggagcg agatactatg atagcgagct
    31201 gagtgcgtgg ctgagcgtgg atccgatgag cgataagtat ccatcgctat cgccatattg
    31261 ctatagtgca gataatccgg tggtgctggt ggatccaaat ggggagaaag tgtatattgt
    31321 aggagatgcg gcagatgagg ctacaggttt tttaattaag aaactatata aattttcaca
    31381 acctccatat aacaaaccat caagtattca tgatcaaata aataataata aataaaaaaa
    31441 tgaaaaaatt aaaggcattt tgtattttat tacttttaaa tctagttgta tctgtgagta
    31501 tcctagctaa taataattta gataataaaa ttaattatga atttttttat caaggcaagg
    31561 ataccatata ttatacaact ggcgagtatc gaataagtag aataaaaagt attaggcatg
    31621 catatttaat atatttatct aattctacaa tggacatttt atacacttta atatctccta
    31681 aatgtaatgt tagattacca gaaaaactaa aagaaggtga gacgtacatt atgcaaataa
    31741 tatatttagt tcctacacaa aaatatgcaa gatacgttgg tcataaattt ataacgaaat
    31801 tgacaatgtt ggataaaaaa gttagaattt ctagaagaaa tctatggggc acaataatca
    31861 tatcacctaa tgtattagat aaatattata ggaaagatca ttttttagga ccagaagttt
    31921 ttaaagaagc aaataattat gaataaaata ttaaataatt gcagaataca aagattattt
    31981 attttaatta ttgtaatttc aaataatatt ttttcacaaa aaaatttaat ttataatggt
    32041 agcttcgaag aatattatag ctgtccatgc aatctaaatg tcgtagacac aataccttgt
    32101 atagggtggt ggtctcctaa tgatgctaca cctgattatt ttaataagtg ctggatagaa
    32161 cttcctcaca ctaatttttg cttgtacaat tacatatatt tagcaagtta caaacccaaa
    32221 acgggaaatg gttatatggg gttaggattg attggagagg aaaatttctg gatggagcac
    32281 atacaaagta aactcataga gccactagaa gcaggcaaac aatataaagt gagtttttgg
    32341 gtgaaattag cttataaata tagtgattac gcagcatata atattggtct ttatttctca
    32401 aaagattcag atattgtagg taaaaaatat aatgtttcta gctacgtgag gtatatgaca
    32461 ccagagttac gagcacatat taaaaaccca gatggcaatt tcataataga tacaaattgg
    32521 gtagagataa gtggcatcta tactgcacaa ggtggagagc aatatatcac aataggtatg
    32581 ttttgggatg ataatcctgc cgtagttgaa gctcataaaa aatacaaaaa taaaaataca
    32641 caatataata aaaagcttct gagtaaagca ataaaaaata atttattagt agaaaatccc
    32701 tatattgaaa aatataaaaa gatggaagat caatacattc gttctcatag tccctattat
    32761 tatcactatt gggataaaga aaaaaagaat atatattatt tcatagacga tgtgagcgta
    32821 atagagttgg acgaatgaat ttttttttag attatatctt aaaaaatttc tttcatactg
    32881 tcccgcccgc attggcaaat gatttgccat aaaacaagca agccgtgttg ggaagaaggg
    32941 tttacactgt ttgagcgagc gaagcgagcg agtttgtaaa ccccaagagg tgattgcgta
    33001 gttttatcaa atcatttccc cagcggagac ctttcttttg ttacttttct ttatgtagct
    33061 gataaagaaa agtaaattat aagttaaaaa taaatttaat attagtaaga catttaatat
    33121 ttttgtttgt acccaagtat gtgtgaaatt atttcgacat gctcaagcga ttattttttt
    33181 taaggtttag aattttataa tttgtatata tgaaaaaaat tttgtattat ttatcaagat
    33241 tatcaactaa aaaaaacaaa ataatgattt ttgttttttc taataaattt tatttataat
    33301 tttgtaaaaa tttttaatta ataaattaat aaactatgaa agcatatcaa gtaaaagaca
    33361 ttcgtaatgt agctattatt ggcagtccta aaagtggcaa aactactttc gtagaagcta
    33421 tgcttttcga tgctaatgta ataaatcgca gaggtaatgt agacgaaggt aacacggtgt
    33481 cagactatag agacattgag atcgaacgag gttattcagt ttattcatct gttttagctt
    33541 tggaatatga aaatagtaaa ataaatgtaa tagatactcc tggttttgat gatttcattg
    33601 gcgaaatatt agcagcttta aaagtttgtg atatcaattt gaatttaatt aatgctgcca
    33661 atggcataga agtcggtaca gaaattttat ggaggcattt ggatagatta aataaatcag
    33721 caatttttgt atttaatcaa ttggaacatg aaaaagcaca attccaagaa atagtaaatg
    33781 atttgaataa ttctttttct aatagaatca taccttttca atatccatta aatgtaggac
    33841 ctgattttaa agaagttgta gatgtattat ctgaaaaatt aattattttc actgatagca
    33901 atggtaatta tacagaaaag gatttaccag atagtgaaaa atccaaagtt gctgatttaa
    33961 aaaatcaaat aatagaatta gtcgcagaga gtgatgagaa attaatggaa aaatattttg
    34021 aaaatggcac cttagattat caagatatcc aaaataattt aaaaaaagct attatatcaa
    34081 aaagtatttt tccagtattc tgtgctagtg ccaaactaaa ttatggagta aaaaatatta
    34141 tgaatttctt agttcatttt gcaccagctc ctaatgaatt accttttgaa aaagctaaaa
    34201 gtggtagaga actatcatat aatcctaatg atccatttgt tggttttgta tttaaaacta
    34261 ctatagagca acatttaggc gaattaagtt ttataaaaat atattctggt actctatcag
    34321 aaggcatgga tgtagtaaat gcaacaaatg gtcaaagaga aagaatcagt caacttttag
    34381 taataaatgg taaaaatagg gaaaaaatct ctcaagtata tgccggcgat atagtggcta
    34441 cagtgaaatt aagatatact tctactaatg atactttgac tagtgcaaaa aacactaatg
    34501 atattgtaga acctattgaa tatcctgaac ctaaatatcg tgtagctatc agagctaaaa
    34561 atactgccga tgatgaaaaa ttgggagctg ttttgcaaga actccatcgt acagatccta
    34621 ccttgcaagt agaatattct aaggaactga aacaaataat tttaagtgct caaggagaaa
    34681 tgcatgcttt agcagctaaa tggcaaatag aaactatcga aaaaattcct atagaatttt
    34741 tagatcctaa aattccttat agggaaacta taactaaatc tgcaagagct tcttatagac
    34801 ataaaaaaca atctggtggt gcaggacaat ttggcgaagt acatttaatg atagagcctt
    34861 atcatgaagg aatgtctttt aatgtagaat ttcctatacg tggcaccgaa gaatacgact
    34921 tgccctgggg gggcaaactt gttttcaata attgtattgt aggtggagct attgatactc
    34981 gctttttacc tgcaatatta aaaggtatta tggaaaaaat ggaagaaggc ccactcacag
    35041 gaagttatgc tcgtgatata gtcgtatatg tttatgacgg taaaatgcac cccgttgata
    35101 gtaatgaaat ttcatttaaa atagctggtc gtagtgcttt tagagaagct tttaaaaatg
    35161 caggtcctag aatcctggaa ccaatttatg aaatagaagt cttagtacca gctgatagaa
    35221 tgggagacgt aatgactgat ttacaaaata gaagaggtat tattttggga atggaagctg
    35281 aaggtaatta tcaaaaaata aaagcaaaag tacctttagc tgaactaaat cgctattcta
    35341 cagctcttag ctcccttact tctggaaaag ctactttcac aatgaaattt tctgaatatc
    35401 agcaagtgcc atctaatatt caagaaacat tgctaaaagc ttatgaagaa aaagatgaag
    35461 aatgatttat cttaaatcat tttaatagct ataatttaga ttaatatttt tataatttac
    35521 tgattattag tttgttttaa tctttgaaat tattaaataa aaatattttt acctttcttg
    35581 ttttaaaaaa ttaatatgaa aattatttgt ataggtagaa attatttaga tcatataact
    35641 gagttaaatg acgcagtgcc acaagagcca atatttttta tgaaacatga aaatgcatta
    35701 ataaaaaata aaaatatgcc ttttttttta ccagattttt ctgatgagat acattatgaa
    35761 gtggaattag ttattaaaat aggtaaagtt ggtaaaacta tacccaaaaa acacgcatta
    35821 gattttataa atggtatcac cgtaggcata gacttcacag caagagattt gcagcgtaaa
    35881 tgtatcgaag aaggtttgcc ttgggagata tgcaaatcct tcgattattc agcacctatt
    35941 ggatcttttg ttgctataga aagattttct gatattaaac aaattcattt ttctcttaaa
    36001 aaaaatgata aaatggttca atatggttgt agcggagata tgattttttt ctttgatgat
    36061 attataagtt atatttctaa atttataaca ttaaaaactg gagatattat attcacaggt
    36121 acaccaaaag gagtaggaca agtaaaaaaa ggcgataaat tggaagcata tttagaaaat
    36181 gaaaaaatgc ttaataaaat tgtattataa ccattttttc tttttaaaat aaaaaatcaa
    36241 taatattgct attaaaacca ttacgcccca gacaataaaa tatccgtatt tatattctag
    36301 ctcaggcata tatttgaaat tcattccata tacaccaact ataaaagtga gtggaatgaa
    36361 aatagtagct atgatagtca aaaccttcat tatttcattc attctagtac taacctctga
    36421 aagatatatt tgcaataaat taaaagtcat ttctcgataa gattctaaac tatctactat
    36481 cctatatgtg tggtcgtata gatctctaaa ataaaaagta gtatcatgaa ttaattttga
    36541 attaagattt acaatatttt ttgtaatctc tactactggg aaaatatatc tatgtaagca
    36601 cgatatatta gacgtcaatt tatgtaattg atttgtaata tcatcattat tatctccaat
    36661 taaatcattc tctaaagtat acaaattatc ttctatacct tgtagtaata aaaaataact
    36721 atctactatc aaatctaaaa aaatataaag taaaaaatca ggttttttag tcttaatatt
    36781 gtttcgatta tttttgatat gatcaataat cggatcaaag aaattagttt gtttttctaa
    36841 aaaagtgatg aaataatttt cgctaagtat acagctaatt tgcttttgat ctaattgtaa
    36901 atcattttca atagcagaga aagatttgat aactaagaaa agataatcat cgtaattttc
    36961 gattttaggt ctctgatcta catgtaatat atcttctaat actagattat gtattttaaa
    37021 ataatcacca atttgcttta ttaaatcttc atcatgaagt ccataaattt gcaaccaagt
    37081 tatgttatca gtgaaattta gttttgttaa atcttctact tcagttattt cgacctctaa
    37141 ataaccattt tcattgtact gaattatttt tatttttata tcgtcatgta tatcacctgt
    37201 gtaaactaaa ctctctggag gcagataaac tttagctttt ttatggggta atttattagt
    37261 aatagtatta aagtttagtt tcacattaaa aaaattttat aaaatttaaa aactatcatt
    37321 taatagtgta tcactagaga aaaaattttc ggcagactca aatattaatt tactgtctat
    37381 agtgtctgta ttcatttgtt ctacactttc tttaatctct ttcttatctt ttttatttat
    37441 tttaccacaa ttagcaaata ataaaataat acatattgaa ataaaataaa ctcttttttt
    37501 cataaaattt tattacaaat atataaaaaa agttttatta aaacaattta acccttttta
    37561 acatcattaa attttaatga tgttaaaaat tctattacct tatcaacctg atcaccttgc
    37621 agtataattt cattattatc tattgttccg cctacagcta atttttgttt tataatttta
    37681 gctaatattt taattttttc ttcatctaaa tctagacctt tgataatagt aacaggatta
    37741 ttagccctac cttttctctg atagatgact ctgattttag aattattaga taaatctgct
    37801 attgatagtt cattgttatt agaggattgt acatcatcag tttcagactc ttcattattt
    37861 gtgtttgtaa gattaaatcc tttttccttt aatagatcac ctattgtaaa ttttttatta
    37921 tctgtattat aactttcttt tttcatttta atctcttatc tgataaaaaa aacaaatata
    37981 ataatttttt gaaattattg atatttagat ttaggaggca ataaacattt caaagctcct
    38041 ttttctactc taatattaaa atgcacatct gtactcatat atggttcacc atctatgtga
    38101 tagtattcct ttgttcgagc ttctaattca caggatgaaa ttctcaatat tgttaaaaaa
    38161 ggggatttat gaagtttctt ttgtaaaaat aaaattatta aacgaagcac attataccat
    38221 ttaaaaggtc taaaaactaa aacatcaagc ttgccatcat cagttcttgc atttggattg
    38281 atagtaatac cataaccata ttgactgata ttagaaaaag ttaataaaaa tggaataaat
    38341 actatattat tcttttttac ttgcaattgc agagtatatc tatgtggttt ataacgaata
    38401 agagctttga taccagcaaa aatataaggt aaaaatctcc tcatagtatg ctgagaataa
    38461 taattagcca aataagcatc aaaaccaaac ccagcattac acaaaaatat ttgattatta
    38521 agataagcta catcaaaatc gataatacga ccatttgtta aagcaggtaa aatatctttt
    38581 gtatttagta gcatattcaa atgtctagcc ataccattac cactaccctt aggtataacc
    38641 aataagggaa tgtctgtatt tactatagcc tccgctacct catttatagt gccatcacca
    38701 ccgacagcca cgactgataa caaatctttt ctataaaaat tttttataaa atttttggaa
    38761 tcgtctctat taccagtagt aaaaaaaaat tcataatcaa tattattatt attacagaaa
    38821 ttttttattt catttattac acgtcctttt ttccgtttac ctgctattgg gttaacaata
    38881 aataatgcta aactcattgt aatttattaa gaatcaatat tatcaattaa taaaataaat
    38941 aatttttatg caaaaataga atttaattta ttattcgaaa taatttttta catcaaataa
    39001 atagaaaaat ttatgataag ccttgtgtat atgattttta gagattgtga aagcatcaaa
    39061 acaaatcatt gttttacttg aagtagttta tctatacttt gtattcacca gccgccaata
    39121 ctcatcccac gcccatctgt ccgaggatgc gaggtttaaa agccgaccaa tgtctgattc
    39181 tatctcactt gccaagtatt tatgtgtgat tacaaaccac atcagatagc tctggaggta
    39241 tttggtagcc actcctctaa atcgccgcaa ccaaaccttg aaatcatcta tatgcttatc
    39301 aacaacctgt acactgtata caccgcgtct aattatttaa ttatgctata aaatacgtaa
    39361 agtaaaacaa aggatatttt taagtaactc cgctcaataa atcaccttta tgaagcgtaa
    39421 tttttacaaa gctaaatttc ctggatctat ttatttaaat tgggatagaa attatagaaa
    39481 aagttctata acgttttgta taggtaaaaa ttaaaaaaga gtgattttat aaaaaatttg
    39541 ttaatattgg tttatttgtt ataggataat caaatattaa ccacaaagtt ctcaaagaat
    39601 gcacaaagaa cacagagaag gatggaatta aaaaggctac aaatggcact ttgtggactt
    39661 tgtgtaataa cttggtgccc tttgtggtaa aaaaatcaac ctaaataata aaaaaatagt
    39721 tattttttgt aataatctaa aattttatta cacaataaac gttatagaac caaaaaaatt
    39781 attgtaattg taaaagaaat cgaaaggggt ctagaaacaa atcattgttt tacttcaagt
    39841 agtttatcta tactttgtat tcaccagccg ccaatactca tcccacgccc atctgtccga
    39901 ggatgcaagg tttaaaagcc gaccaatgtc tgattctatc tcacttgtta agtatttatg
    39961 tgtgattaca aaccacatca gatagctctg taagtatttg gtagccactc ctctaaatcg
    40021 tcgcaaccaa accttgaaat catctatatg cttatcgact acctgtacac tatatacacc
    40081 gcgtctaatc tgcgttttgt ttacaacaac ttttttgctt ttggtcc
//
LOCUS       DAOPRN010000012        38115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_832_length_38115_cov_98.958592, whole genome shotgun sequence.
ACCESSION   DAOPRN010000012 DAOPRN010000000
VERSION     DAOPRN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38115
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_832_length_38115_cov_98.958592"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..2071)
                     /gene="uvrC"
                     /locus_tag="PKI65_02485"
     CDS             complement(275..2071)
                     /gene="uvrC"
                     /locus_tag="PKI65_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOB77342.1"
                     /translation="MNEGQISIILKTLPKSPGVYRFYDKDDNILYVGKAKNLKSRVSS
                     YFRTQHNSARLRIMVKKIERIETIVTNTELEALLLENNMIKNLKPRYNMQLKDDKSYP
                     FIAITDEPFPRIFLTRNRHIHNAEYYGPYASVRTAKSLLELMHKIFPLRTCKLNLSEK
                     KIKNGNYKVCLEYHLKKCKGPCVGLINEIEYDHYINLARDIIKGKTKIVENFLYDKMQ
                     KEAENLNFEEAHQWKIKWEKLKSYSSRSIIVTTQENNLDAINLIENDNEAYISAIRVQ
                     NGSIVNSIIFHIEKKLNETSEELLLYAIGELYDQWDGLQPELIVPFPISFPIDNVHIT
                     VPKIGYKADLLKFCLRNAQERKTENDKRRAITDSNKYINQLLEKVKSDLHLPTLPKRI
                     ECFDNSNIQGTYPVSSCVVFIDGKPAKSEYRIFNVKTVKGINDFATMHEVVYRRYKRQ
                     TEENKPLPDLIIIDGGKGQLHSAYDALEKLGLENIPIIGIAKKLEEIYKPHDPIPLNI
                     DKKSPTLKLIQRARDEAHRFGITHHRRLRSKDTIKTQLVDIKGIGPKTATKLLEHFGS
                     VENIKKAKDEEIETLIGKAKLKILREALQDKD"
     gene            complement(2096..2866)
                     /locus_tag="PKI65_02490"
     CDS             complement(2096..2866)
                     /locus_tag="PKI65_02490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOB77343.1"
                     /translation="MHKNENKLSIVIITFNEEKNIARCINSVKEIADEIIVVDSYSTD
                     KTKDIALDLGAIVIENKFEGHIEQKNIALQKANYTWVLSLDADEALSDKLKNKIQEIK
                     KNPTADGYTMNRLTYYCGRWIRHTGWYPDKKLRLVRKDLAQWCGINPHDRLELTNSNN
                     IKHINADILHYSYFTLNDHLNQLEKFTQISANELYKYKIKPNFWHFFIKPLHKFINHY
                     IYHAGFLDGYEGFQISMISAYGVFLKYAKLRELYKTNK"
     gene            complement(3081..3641)
                     /locus_tag="PKI65_02495"
     CDS             complement(3081..3641)
                     /locus_tag="PKI65_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOB77344.1"
                     /translation="MFRDFIIPFLLIGVAEFADKTQLLIIVIASKTKKYFALFFGVLL
                     AFAIVDGLAILAGGLIAELIPKNIMTIIAGLIFLIFGIITLVRYEKEINFTKTNINPF
                     ITGFLLIFLSEWGDKTQIAAATLATQYNPPLVFLGTIIGISVMTILSIFLGNILMKYV
                     KAKTLSIVSGLLFIIIGVFTIASIYF"
     gene            3785..4840
                     /locus_tag="PKI65_02500"
     CDS             3785..4840
                     /locus_tag="PKI65_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOB77345.1"
                     /translation="MKINLVTGACGFVGRNMVKRLYSTTTDHVFMVDDLSIGIHPSEW
                     FPNYYPSDYNGLEVLDPDKRVYFWKGDLRELLFNFRENPRYIQETYKLDFDKFSDVFH
                     FAAIVGGRLKIDGDPVQVGLDLSIDAELFYWAVRNMPDRLLYPSSSAAYPVDLQTEET
                     ALALKEEYIDFDSLRIGVPDMTYGWSKLTGEFLAKIAAKHYGLKVVCIRPFSGYGEDQ
                     DLSYPVPALAARAAAKEDPFVVWGTGKQARDFIHIDDIIDFIFVLMDNVHDGSGWNIG
                     SGKLTSFLEIIDLFTKFAGYKPTIKPLIDKPMGVHSRYADMTKVFSTFDWRPKVSLEE
                     GMYRVYKAQLEKLNKNK"
     gene            4846..6132
                     /locus_tag="PKI65_02505"
     CDS             4846..6132
                     /locus_tag="PKI65_02505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB77346.1"
                     /translation="MRIICFGPGPQFKGGLANYNTSLAKAFSEFDKAEVHIVSWTNQY
                     PSIVPRDFIDRKSRYNALEGTDIKVHYLTNYNNPFSWIKTAKLIKDINPDIAIFQWSI
                     AMQGLPLRYIVTWLKKHSNIHIIFDLHFVVQKESSSLDKFFTKFALKHVPHFIVHSKK
                     TFDELCDVLPKRQFQLVQEFNPKKSDSQVIHLYHPIYDMFKPNEDFDVETFKKELGLQ
                     KYVFLFFGFIRKYKGLHNAIKAFAELSKDRDDVSLLIVGEAFWSTLDKKKWTTRVKKW
                     LFNVAQSIFRKDTESEDNYRPLDLVKELGIEDKVLSLIEYVPNEEVYKYFQASDAIVL
                     FYEYATPSGVESMAYNFKIPIIATAVGHFPETIINGVNGYLADPDDIKSMAEAMKKII
                     EKPIHRANIEKIANEKSWHAYAEAIVKNIYTNFTNK"
     gene            complement(6234..7097)
                     /locus_tag="PKI65_02510"
     CDS             complement(6234..7097)
                     /locus_tag="PKI65_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOB77347.1"
                     /translation="MRQLKITRSITNRDYTSLDDYLQEIGREELITPEEEVKLAQRIK
                     QGDEAARNKLVKANLRFVVSVAKQYQNQGLSLQDLINEGNIGLIKAAERFDETRGFKF
                     ISYAVWWIRQSILQAIAEQSRIVRLPLNQIGSINKISKESAKLEQELERAPSVEEIAD
                     LVDIPESKVAESIRISGKHVSVDAPISPDEDATMLDLFVPSDAPSTDHSLMQESLSKE
                     IQRALSSLTKKERDIINMYYGIGYAHPYSLDEIADKYKLTRERVRQIKEKAIKKLKNG
                     NRSKNLKAYLG"
     gene            complement(7223..8689)
                     /locus_tag="PKI65_02515"
     CDS             complement(7223..8689)
                     /locus_tag="PKI65_02515"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOB77348.1"
                     /translation="MKKEKVEKFWNYFQKVMSVCTLVLFFIVIYSFTASPSKASENRN
                     NYNYEKYAIIGDTIKFPDFRYAAKKAIPAVVNIKTTFTFKNIYYDDFFAPFYDFFKFF
                     GGPSREQTIIGGGSGIIISPEGYIITNNHVVQDANIIIVTLNDNREFQAKIIGNDPST
                     DLAIIKIDAKNLPYMEFGNSDDIEIGEWVLAVGNPFNLTSTVTAGIVSAKARNINILE
                     SADAVESFIQTDAAVNPGNSGGALVDIYGNLIGINTAIATKTGAFIGYSFAIPSNLAK
                     KVANDIINYGFVQRPYLGVSITDINSEIAKKYNIDEIKGVLITAVTPNSAAFKAGLDA
                     GDVILKLDGEEINSASRLVEKINEKNIGDWITVEYKTGNKVKTAKIQLLNKYGDSKPV
                     KREDMTVQAILGASFEKPSPDLMAKLNLDHGLQIINIEGGPLQRAGIKKDFIITEIDH
                     KPINSVEDIEKALKNRKGAVLIEGVYPNGIRAYYAFTL"
     gene            8818..9276
                     /locus_tag="PKI65_02520"
     CDS             8818..9276
                     /locus_tag="PKI65_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77349.1"
                     /translation="MNKKGYFFVIGIIGGIILCIILIYAFLPRFKNLVYQPEFFYYSD
                     IIDEYEINKKRSFDMMESRFRKLYKSNFKLLINDSINNNNNDSLTSAKILNIKNKDTI
                     FKLLYYNILDSLLKISGYKYVDSITLDSLVKVMILDSIEKIKIDSTKVFF"
     gene            9357..10499
                     /gene="dnaN"
                     /locus_tag="PKI65_02525"
     CDS             9357..10499
                     /gene="dnaN"
                     /locus_tag="PKI65_02525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOB77350.1"
                     /translation="MKFIVSNAELSKAINSISSVPIANNVIKILENFLFKVNNSKLTI
                     TATDGDTRASVTIDLIESDGEGEIVMPSKLLQEYLKNVPYIPIAFNMDTTGENNYQIT
                     ISAGSGVYRMVGMDAENYPTMPQIPDARTVKLNSTVLSTALEYTLFCAAVVDYRQILT
                     GVNFDFRDEKLNVVSTDSHRLVRYRRFNIETEEDFNFVVKRKPLQILRNLIDTDTDVE
                     INFNETNVSFEFENIMISCKLLEGVFPDYDRVIPPTFENKLLIDKESFISALKRVAVF
                     TDKATNQIRMHIEGQEITLSGNDEEYANEGIEKLPCTYEGQDMDISFNSKTFMEMLQH
                     LDCDNIYILMNKPNTAIVITPAEENESEDIVMLHMPISLKGSEDYY"
     gene            complement(10585..12066)
                     /gene="dacB"
                     /locus_tag="PKI65_02530"
     CDS             complement(10585..12066)
                     /gene="dacB"
                     /locus_tag="PKI65_02530"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOB77351.1"
                     /translation="MKKLIIIFALLSVFNLSAQSLNESFILSPLNNDHRVDKNSYSFI
                     LYSLSKSETLLSINQYKPLIPASVNKLVTAISSYYYLGDSFRFTTNAYLSGYQKNNVW
                     HGNLYLFGTGDPLIGEDFPEGNKFFDSLVNILKQMGIDTLYGNIIADASYFGDEIIPK
                     TYEYEDIGNYYGAGASSLMYNGNTWKIAYKLNQEYGKTAKLIYTEPVFPHVNYQNRVK
                     VANRSYGNYTHVSGKPLEKNYIFSGTIPRGKGETLFVKASSPDPAYGFAWKTQNTLLI
                     NDIFLIGTLKASYTDTISYESKKLIFEYKSMPLDSIISFTLTHSYNVGAEILAKAIAK
                     KLSDGSYESYGSLAKQLIYKFGSDTTDINICDGSGLSRKNKLTADAVLTLLIASYNSN
                     WFHSLYNSLPVAGINGTLKKFLKGTPLENNLRAKSGYLRNTRAFAGYFDLPDGMDTIA
                     FVTIVNNYNGDTQYATKNIEAILLNTYNDLTNKYRASKVDENL"
     gene            complement(12191..13648)
                     /locus_tag="PKI65_02535"
     CDS             complement(12191..13648)
                     /locus_tag="PKI65_02535"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOB77352.1"
                     /translation="MKSFIKIFSLFLIFISWDATAQDYKDYKMSKHEAYIDLAHLYQY
                     LCNAHPNISEVADTVFLKNAFANLFFSIDDSVSINEFFVRLAYIFSKIDDSHLMLNLN
                     YYAAQKVFPKFFGGVVAFNDGKIYLLKGVSNNKFFNNTRILSINDIDASEIYSTLANL
                     CNADLGLRTTKDNNASLLFYYYFPFIYQVKNINNCVLLDEINNDTIKYIIDGISMRDI
                     KSSQSFFAPYIEDLSNPYKFKLYPTINMGYLYVSDFLNNKYNTPDQLMPIVISNLNKY
                     NISYLILDLRGNPGGYVKVAEDFLKYLMPAPFTYVKTYRVNPSKWTDQLLLKNSLLQK
                     EWYNFILTLKSIKNKELKLLKAGYKGPSVYEINKETKPHKNHFNGTIYVLTDGGSISA
                     SSLAANLLAHRPNTYLVGLPPGGTKKGTFGQSKIFLLPHSHISVSLSLLEFVQFPEYD
                     REPIDIHYPLEWAQPNFNDPIKDPWVQFVIKNLLK"
     gene            complement(13793..15466)
                     /locus_tag="PKI65_02540"
     CDS             complement(13793..15466)
                     /locus_tag="PKI65_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOB77353.1"
                     /translation="MRTSISKNKFFLFIIKITNNKLIYFLILLNFINFSCNKNYKTEI
                     NNNKQIFHYNQPAGLYSLDPAFARDQAHIWVVQQLFNTLVSFDDSLNLLPSLAKSWEI
                     SHDGCRYIFHLRDNVFFHNDKCFENETRKLIADDVVYSLQRLADEKTASPGRWVMSPI
                     STNNNNLSVKAINDSTVVIYLKKPFTSFLSILAMPYCSIIPREAIQMYGESFSHHPIG
                     TGPFVFKYWKKDVKLLLAKNENYFEFDSLGNKLPYLDGVSISFIKDKQVAFMDFLLGD
                     FDFLSGIDPSFKDILFTDNGELKPEFKNKYIFQKSIFLNTEYLGFLISDDTPFNNKEL
                     RKAISLGIDRISMMKYLRNNLGYPALYGFIPPILNPLDSSNKYLNYDPHYAKQLLIAS
                     GYMDMPNKPKITLLTNDTYIDIAEYIQNQLSKIGLDISIELMPPATLRDLMFKGQAQF
                     FRGSWIADYPDAENYFAIFFSNNIPPKGPNYTHFKNNTFDELYLSLLKTSNADKYEII
                     KQMNKILIEEMPVVPLYYDVSIRLLQPYVKGLNTNPMNNLNLKYTKIVN"
     gene            complement(15600..17132)
                     /locus_tag="PKI65_02545"
     CDS             complement(15600..17132)
                     /locus_tag="PKI65_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOB77354.1"
                     /translation="MEKRKDIRQLIIDAAEQYNDRTAFIYNDKEFDFNTLKNDSFRLA
                     NALLDLGLKKGDKIAIYLPNCIEYVYSYYAIYSLGCIAIPLDFYLSANELISIAKHCD
                     LSAIITTSKAKFDLSELKDSIPTLQNIITIEDKTNYIYFWDLINTHDNNLNDQNVTSD
                     MISSIFYTSGVTGRPKGALWNYEHIQLTAEQIKFFTTNEELKGIINFDENERIIAAIP
                     FSHSAGIFFPMTAIKYGVSSVIMPQFHPLQLVKLIDKWQVTGVFMVPPMYYAVLYLKD
                     IDKFSLNSLRWATVFGAPSSPDLIRKFEKLCPNAIVINGWGMTEVIPPVCASSPSNIR
                     SVGKPVPNVELKIVDPDGNEVKVGEVGELIVRGKSVFCGYYNEPELNNEVFKDGWFYT
                     GDLARKDEEGNYYIIGKSKDTIKVGGQLVWAAEVEEVLLRYPGIREAAAIGVPDELRG
                     EVVKCYIAPVDSIDLDKNELMEYLRNNLAKFKLPKDIIILNELPKTGSGKVNKAALQE
                     FN"
     gene            complement(17847..18050)
                     /locus_tag="PKI65_02550"
     CDS             complement(17847..18050)
                     /locus_tag="PKI65_02550"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOB77355.1"
                     /translation="MKKNNLDKYIKYSNLAIQMLVIILGGVYLGKWLDEKNNANYLYT
                     SILGAIGTLIAIYIGIRELLKNK"
     gene            complement(18063..18416)
                     /locus_tag="PKI65_02555"
     CDS             complement(18063..18416)
                     /locus_tag="PKI65_02555"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOB77356.1"
                     /translation="MAKNIPEVEMTKVNLIGESTRIEGDLATEDDLRIDGIVNGNINS
                     NSRIIIGPTATINGNIKAQNIEVNGKVTGNIEAYDILILKESSELTGDINVFKLSIQA
                     GALFNGKCTMKVKSE"
     gene            complement(18492..19280)
                     /locus_tag="PKI65_02560"
     CDS             complement(18492..19280)
                     /locus_tag="PKI65_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOB77357.1"
                     /translation="MKLYIILIISGLIVGFINTLAGGATIISISVFNWLGLPLTIANG
                     TNRVAIIMQTLSSNAVFFKNKVIPYKNIWRQALAIVIGSLIGAVFAVNVKEDFFRYAL
                     MVVIVIMIVSMFINPKSWTVGKENNANFKRNEWLTIIVFFIIGLYGGFIHVGVGYYIL
                     FAGIYLMGYDVIKASAFKNFLVFFYVSISFIIFIINGLVNWEYGLVHGIGNIIGAQIA
                     ARISLTKSINFVKWLIVAIMILIFIDLTGIISLKQLVLGSLGNV"
     gene            complement(19306..19803)
                     /locus_tag="PKI65_02565"
     CDS             complement(19306..19803)
                     /locus_tag="PKI65_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOB77358.1"
                     /translation="MPGNSKRYLVLMRHAKALPHNFHTNDRERKLSEEGEKQAKLSAE
                     YLVTINFIPQLIITSSASRALQTAKIVSEILNLSNNIIITNDELYYGDIDAYFYQIYK
                     VDNNVKKLMLVGHNPVIAMLAAMLTDYSINKMKTASLLGLEIFSDTWADFDQKTKKIT
                     FLFTP"
     gene            complement(19793..21070)
                     /gene="glyA"
                     /locus_tag="PKI65_02570"
     CDS             complement(19793..21070)
                     /gene="glyA"
                     /locus_tag="PKI65_02570"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOB77359.1"
                     /translation="MTRDVKIFSLIEQEFQRQTEGIELIASENFVSSQVLEAMGSVLT
                     NKYAEGYPQKRYYGGCQIVDQTEQLAIDRAKELFGAEYANVQPHSGAQANMAVFFTCL
                     KPGDKFMGLDLSHGGHLTHGSPVNFSGIIYNPVAYHVNEDGYVDYDEMEKIAIQEKPK
                     MIIAGASAYSRDWDYKRMRQIADSIGAILMADIAHPAGLIACKYLNDPLKYCHIVTTT
                     THKTLRGPRGGMILIGKDFENPWGITTPKGKVKMMSELINSAVFPGTQGGPLEHVIAA
                     KAVAFYEDLQPSYKEYINQVMKNAQAMASAFVDKGYHVISGGTDNHLMLIDLRSKFPE
                     LTGRQAENTLVNADITINKNMVPFDSRSPFQTSGIRIGTPAITTRGLKENHMPIIVDL
                     IDRVLNDIENENNINKVKSEVNKLMKDFPLYAW"
     gene            21148..22335
                     /locus_tag="PKI65_02575"
     CDS             21148..22335
                     /locus_tag="PKI65_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOB77360.1"
                     /translation="MIWKLVEKIEAKATQWYHQIHNNPELSGEEVETAKYITKLLRSW
                     GISVRTGVAGNGVVGIIEGKEVNGKVIGLRADMDALPIDEKTNLPYKSKKPGIMHACG
                     HDVHVANLLASAYILNEIREKWNGRVVLIFQPSEETLPGGALKMIEANVLDDPYVDVV
                     IGMHVSPELSTGTIGYKSGVFMSSTDEIYIEVKGKSGHAALVKEFIDPVVSSAAIIIN
                     LQQIISRRNNPITPTVLSIGKVIANGRTNVIPSLVNMEGTFRTFDENWRNEAKTLINE
                     IVYNTAQAYATTANVNILSGYPTLYNDEKLSAEIINFIKNNLSEIKLIEVPMRMTSDD
                     FAYFALQRPSFFFRLGTGNEKKGTTYQLHTDKFRIDDEALKYGIYTMSSIAFGFLNNQ
                     INI"
     gene            22336..22827
                     /locus_tag="PKI65_02580"
     CDS             22336..22827
                     /locus_tag="PKI65_02580"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77361.1"
                     /translation="MKNKYLFIKLFKLVFILILMSQILYSCKNKNKIIFSKEVVFINN
                     LWEKDHTLSFLFQITDTSAKYDALIKVMLDTNYTFNTFVYSINLITPDGSLRQFGFQT
                     DIKDYDNNIELPIIGDSWLIDKKVFEDIKFNKYGQYKIELKHNMQLDLFFVKSVELII
                     SKK"
     gene            complement(22828..23949)
                     /locus_tag="PKI65_02585"
     CDS             complement(22828..23949)
                     /locus_tag="PKI65_02585"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOB77362.1"
                     /translation="MKEQNFLFSYTPKLIIDNKIIKNNINKIVNKTQEWEVSLRPHFK
                     THQSDVISFIFENFGINAITVSSIDMAYRFINEKINDIFIAIPINIHSLNRIDYVLDD
                     EYLNRLIVSIDSIEAFRYLIPYLEENTKLNFAIEIDCGYHRSGISVSEEDEIYSIINE
                     IISNNLANKFYGIFSHFGQSYKLSTDDQLYELNATNIELLSNIKRIIEAMLDKSISLS
                     IGDTPSLRIYNKKLLEKVDEIRPGNFVFYDAMQYLHGNCSLEDIAIFLYAPIISIHPE
                     REEIIMHGGAAHLSKDFLIDEGIMKFGLVATWDLKKLTLIPNAYVKSISQEHGVISIN
                     KEFINKFKIGDHIAIIPVHSCLAMDTMVYKDGVFYWEDT"
     gene            complement(24033..25229)
                     /gene="hemW"
                     /locus_tag="PKI65_02590"
     CDS             complement(24033..25229)
                     /gene="hemW"
                     /locus_tag="PKI65_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOB77363.1"
                     /translation="MANSINDNYSINELLVNKNYTNPEHLYIHIPFCKKRCPYCSFYS
                     TIYDNNDNTKDLFISALLKEIKLKDFLIAKNIRTIYLGGGTPSLLTSKDLEQIFNVVL
                     KNHTLDDDIEITIEANPDDITKDLVSEWKNLGINRVSLGVENILEKHLKFLGREHTAS
                     NIRNAINIISKQGINNLSVDFIIGLPDETIDDLKENFKLLINNDIPHFSTYLLTIEEK
                     TRLAKLIKNNPKLMLDDDKIINNTLQWYQWASENGYHHYEISNFSLPNYKSKHNLAYW
                     IQKNYIGFGPSAHSYINPVRFWNVTSLKKYLIGLENNSLEYDYEIINKNEAYNEYVMT
                     RLRLKEGIYFDDLKNKFGPKRAQYFANQACKISQKYLHKDQNRYYLTERGWLIMDKIL
                     VELFII"
     gene            25391..26491
                     /locus_tag="PKI65_02595"
     CDS             25391..26491
                     /locus_tag="PKI65_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOB77364.1"
                     /translation="MENFWTYTLWGNTIKDYLISICAFTITALILWLFKNVVLKRLKK
                     VTKRTKSKIDDILVSCLTVIKWPLYLVIALWVAFKFIVISDKLNQIYNYFVIIVIVYY
                     ATRIIQELINYFTTDISNKRKEEGRATDAGIIKLINNILKVILWVVAILIVLENFGFN
                     IKTILAGIGIGGIAIAFALQAILGDILACFSIYFDRPFDIGDYITFNDISGNVKKIGI
                     KSTKIDSLWGEEIIVSNKDLSNNIVKNYKRMQKRRIEFFFGVLYETPIEKLKMIPDIV
                     REVIGEIEICQVDRVHFYKIGDYSFNFDVVYYVLDPDYSKYMEVQQQINFALKERFEK
                     ENIVFAYPTQTVYLNNNNNEVTKSYVINNKVL"
     regulatory      26973..27068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27130..27903
                     /locus_tag="PKI65_02600"
     CDS             27130..27903
                     /locus_tag="PKI65_02600"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOB77365.1"
                     /translation="MEQIVSNGIIDSYFNKLKQYLSVDVAIVGGGPSGLVASYYLAKN
                     NYKVAVYERKLAPGGGMWGGAMMFNEIVVQRAALHILDELSIKYREFENDFYVIDSVH
                     AASALIYNATKAGVKIFNCTSVEDVVFLDNKVCGIVLNWAPVVRERLEVDPLVIMAKA
                     VVDSTGHDCAVAHTLERKNNITLNTETGKVIGERSLSINEGENSTIDNTKEIYPGLFV
                     TGMAANGVSGSFRMGPIFGGMILSGKKVADLIINKINKD"
     gene            27943..28557
                     /gene="thiE"
                     /locus_tag="PKI65_02605"
     CDS             27943..28557
                     /gene="thiE"
                     /locus_tag="PKI65_02605"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOB77366.1"
                     /translation="MRNFGLYVIITSPQLPYKTIAEKCVYHRIRMLQLREKNLSDKEL
                     IRVGKQIREITRGTYTNFVINDRPDIAVICEADYLHLGQDDIPIEEARKIVGNMKIGL
                     STHSIKQAKDALSKKPDYIGFGPIYPTNTKAIPDKPVGVDQLKEVLQFSNVPVVAIGG
                     IDNSNIINVINTGAQNVALVKYLMQTNELEKRIIEINKLINNKS"
     gene            28554..29834
                     /gene="thiC"
                     /locus_tag="PKI65_02610"
     CDS             28554..29834
                     /gene="thiC"
                     /locus_tag="PKI65_02610"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF009895.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOB77367.1"
                     /translation="MSTQLQKARQGIITPEMQEVANMENVNAEWLRNEIAEGRIVIPK
                     NINHKFIAQGIGNGLSTKINANIGTSSKHCDLKEELEKMRIAVEYGANAIMDLSTGGD
                     LNKILTKIINESSVMVGTVPIYTVITNLLSANREISQLDPEELFAEIENQAQMGVDFM
                     TLHCGVTKSSLAFMNNDKRVLGIVSRGGSLLKRWMIENNRENPLYDQFDRVLEICAKY
                     DVTISLGDGLRPGCGEDATDRGQIAELLILGELVERAREKGVQVMVEGPGHMRLDEIE
                     ANVKLMKKVCCDAPFYVLGPLVTDIAPGYDHIVGAIGGTVAAIHGADFLCYITPAEHL
                     CLPDVDDVREGVIAAKIAAHSADIVKNINNSREQDFIISKARRELDWEAIFENSIDPE
                     LAKKRKLDSESAEEDHCTMCGLMCAIKNDKESSF"
     gene            29924..30685
                     /locus_tag="PKI65_02615"
     CDS             29924..30685
                     /locus_tag="PKI65_02615"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HOB77368.1"
                     /translation="MIYFLTIAASDNSGGAGIQQDIKIADRLNCWPLSAITGITVQNF
                     NKIFTIEPVSPDLLKYQIENCINYFPINSIKIGAICSKENIMIIAECLKKFPHENIVI
                     DPVIFSTSGAQFLDNESLTILKQELFPLAHLVTPNRHEFELLVGQSINSLDEAVKIAK
                     NKCNEWNTAILLKGGHFEGDIIREALITHTSIYYFERVRDIFHYSHGTGCTLSSAIAC
                     YLGMGRTIYDSYLLASELLVDFYKNIQKDFNPLIS"
     gene            complement(30771..31724)
                     /locus_tag="PKI65_02620"
     CDS             complement(30771..31724)
                     /locus_tag="PKI65_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77369.1"
                     /translation="MWERIDVIFTLKSPLHIGYLPFKGSVISPTRYYVPGKNFWGAIT
                     KRTTEHLYKDPHGSDYREVGDKIKNNFRFSYFYLYDDKTIYTPSFADDELKYGNLLLP
                     EFEHKFIGSRVLTAIDKNSGTAKDESLHEVEFIKDKYIDENGEVKTTKIIGCIWVKEN
                     SKLGDYEVQIKNDGIFVNNFNLIEELTLGGEQGYGFGLVELESTLNNKVFPIEEKNLN
                     GDDFLIEIKKDQPIISHLQYDKSLQFRGDIEPITGRGYFDIEKNTESESENEYKKIPG
                     KSLSKINYFFSPGSIIKLENNNTVSLNWNGILKVHNSNKTN"
     gene            complement(31708..32091)
                     /locus_tag="PKI65_02625"
     CDS             complement(31708..32091)
                     /locus_tag="PKI65_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77370.1"
                     /translation="MINLDYICAKIGQNLVENEKDKKENTLRKALGILQEDGVYAMFL
                     WLENKDNEIRTKLNSLLNEKEITSKLLSDSKSFPDDFKGFCEKLQEVAKDIDKLLFLK
                     KILERTLTYALYHAKIKEEENVGKN"
     gene            complement(32078..32992)
                     /gene="cmr4"
                     /locus_tag="PKI65_02630"
     CDS             complement(32078..32992)
                     /gene="cmr4"
                     /locus_tag="PKI65_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HOB77371.1"
                     /translation="MNEPKKYFGITLDPVHIGAGGYRLGRVDNTIVRDPATDVPKIPG
                     TSLGGAIREFATVYTMKNACQSKTDEKEKRKYAEEEVKKYFGDENRQGVLRFYDGQII
                     FFPVSSIQGTVWITTKELLEYWLCEIKDEKGETITIPEEIKDEAYAIKGINSSEPLNF
                     GWLILDTQNINEGKEITLPPELDKFLKRIVIVSDKLFSHIVNDNLEVRTSVRIDPETG
                     TAAEGALFTYEAIPRGTILGFEMVTDTRKEDNVESIVNLIEATFPYLKLLGLGGMGTR
                     GFGRVDFFTLKNNKQSNNKMEGNEDDKS"
     gene            complement(32989..>34631)
                     /locus_tag="PKI65_02635"
     CDS             complement(32989..>34631)
                     /locus_tag="PKI65_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated protein Csx11"
                     /protein_id="HOB77372.1"
                     /translation="TIWLDEIADTNNRVALLTLNFDLEKWLDGTIIGTIFSQTFEDWK
                     NSNDAIKFFNNKQNTQKLEKQGIKIDDTFINLSKNLLKIITDENITKDPGFKSNIINT
                     FYEDIKSNQTIEDENYVEKFINNINERIKPAQFNSCNLQKLIFTQNPSPARLYRIWKE
                     TKEFFDLIINKINSEIYQNKWKRIKFSLDYTDLESKTPFVIKINNLNPQNLLVFHSTN
                     GEFYTIESLEKYRCGEKSGVEAVKEAFRKGINWISTEDAPDTNLLQNDQNVKIKNQIS
                     EEYYYPFIEITKSPLSLRLLVPASDSIKILESIVKLYNQRFEKVIGKLPLNMGLLVAN
                     RKFPLYMLLEAGGRMFKNKEFKDPESMDVWWDINGLRNDEYYSFYPVVNKKDSYNLDD
                     LARLSKGGKFALYPGYFDFDLLLGTDDRYNIFYKDKKRAGKDYKLYSKRPYYFYQFAE
                     MMDLWEILTNNIPGSQINFIEEMFTNKLREWRNVSDANQEEVFTKFAEATIKDAFSEN
                     WNNLRIEMQDLILNSAYNGLLLDTIILFNHTIKGGQTK"
     assembly_gap    34632..34731
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<34732..36056)
                     /locus_tag="PKI65_02640"
     CDS             complement(<34732..36056)
                     /locus_tag="PKI65_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx11"
                     /protein_id="HOB77373.1"
                     /translation="MRTGEEMLDIIRNDRVHILTGEIGALLHDIGKCHPDFIGKNSIE
                     NTPTNFDHTNIDEFLENKFVDLVKSSKLEFIINNENTSVYSLIKEHHNRNDNIIINIL
                     KSCDRLDSADDKGIVRRKQSKDNAIISSPFGFDKEIIELKCLQKRLDDLQSNLTGLFQ
                     NYISENMSIDCFRKSLINNLKTTFSHALGETRIPANDVTLWDHSHSTASLFKSVLCAM
                     VFGENPDNSKLQWRILGFCWDGIGFINKGRKIADILNRNEIIEDIKNNLTEKFEVEIP
                     IGNVIYEDINGIYFTFPALNENSEVLAVECAKEALKIIYNISNNEIWPFFTLSTPSRS
                     LTILAGELKFAAENRNIPKMSPRLFIDRKEEKLLVSLDMPTPKDEDEDKNKVDICPVC
                     KFRAKSIENERCNVCEERKTGRLNEWLSNRKNTIWLDEIADTNNRVALLT"
     gene            complement(36059..36886)
                     /locus_tag="PKI65_02645"
     CDS             complement(36059..36886)
                     /locus_tag="PKI65_02645"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOB77374.1"
                     /translation="MIHDYYLSLTKLVKNIDDQISEVGKKKKEIMGKLNDHQDYNFYP
                     TNSDLKNLPEDSTLLKISFTLKKPYTSKDEGEFNIIDNKIFENPIVRDKFTGLPMMKP
                     TTWKGHLRFAANKVEWQNEQEKKIIIKRLFGSEPEEKDNPLKGRLYFFPTFFNEDAEK
                     DVITPLKRDTRTPTKRGPISLEVMKPGKKGDFHLLYFPYPRGDEFKEEEIKKDLPFLA
                     KALELMFYTYGFSAKKTSGFGVIERLKDSEVEVKPCDKRELFSILYTEHNENIKDGS"
     gene            complement(36883..37632)
                     /gene="cmr1"
                     /locus_tag="PKI65_02650"
     CDS             complement(36883..37632)
                     /gene="cmr1"
                     /locus_tag="PKI65_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr1"
                     /protein_id="HOB77375.1"
                     /translation="MNKYTVKIKALTPIWTGDADRKNTTLRETGIIGSLRWWYEALIR
                     GLGGTACDPTNTNCNGDNHCDACELFGCTGWARKFRLEVEKKNDNETIDLRFIEMREM
                     KNIEWALLNKTIKIISDYGAIGGKIAEAEYGIIKIEQNGLDKFDLKKEELNNYLRKKV
                     KGVDNPNLSRFIFIKNQNNKTFIEIKNNLSFLKGAKNKGKKYFYKTINKKPNRLFVYA
                     EKDDEFNCIKKFLNKKPILFIEGLKLLEELK"
BASE COUNT        12684 a         5666 c         5683 g        13982 t
ORIGIN      
        1 agattatcta gaaatctacg tttttgttct ttgggtagag cttgataatc taatagattg
       61 aagttatcca taattatttt tttgtaaaat taaataaaaa ttatttgatg gtagtataaa
      121 ctacgtaaag taaaacaaag attaatttca ggaagcctcc caaccaataa aatacttctt
      181 tgcaataaat aataagaata agagtttcaa acttaatatc tctatgtaaa ttataataaa
      241 attttttaca tttttgaaaa tttaaaaata tagtctaatc tttatcttgc agagcttctc
      301 tcaatatttt tagttttgct tttccgatta atgtttctat ttcttcatct ttagcttttt
      361 taatattttc taccgaaccg aaatgttcta atagttttgt agctgtctta ggtcctatac
      421 ctttaatatc tactaattga gtttttattg tgtctttgct acgcaacctg cgatgatgcg
      481 tgataccaaa tctatgtgcc tcatctctag ctctttgtat gagcttaaga gtaggacttt
      541 ttttatcaat attcagaggt ataggatcat gaggtttata tatctcttct aattttttag
      601 ctatccctat gattggtata ttttctaatc ctaatttttc taaagcatcg tatgctgagt
      661 gcagttgtcc tttgccacca tcaattatta ttagatcagg taggggttta ttttcttctg
      721 tttgtcgttt atatctacga tagactactt catgcattgt agcgaaatca tttatccctt
      781 tcactgtttt aacattaaaa atacgatatt ctgattttgc tggtttacca tctatgaata
      841 ctacacatga tgatactgga tatgttcctt gaatattaga attatcaaaa cattcgatgc
      901 gtttaggtaa ggtaggtaag tgtaaatccg attttacttt ctctaatagt tgattaatat
      961 atttattaga atccgttatt gctctacgtt tatcattttc ggtttttcgt tcttgggcat
     1021 tgcgtagaca aaattttaat aaatcagctt tgtaacctat tttaggtaca gtgatatgca
     1081 cgttatctat tggaaatgaa ataggaaatg gcactataag ctctggttgc agaccatccc
     1141 attggtcata caactcacct atagcatata gtaatagctc ttcactagtt tcatttagtt
     1201 ttttttctat atgaaaaatt atgctattaa caatagatcc attctgcact ctaatagctg
     1261 atatatatgc ttcgttgtca ttttctatta aattaatagc atctagatta ttttcttgag
     1321 tagttactat gatacttcgt gaggaatatg atttaagttt ttcccatttt attttccatt
     1381 gatgtgcctc ctcgaagttt agattctcag cttctttttg cattttgtca taaagaaagt
     1441 tttctactat tttcgtttta cctttaatga tatctctggc tagattaatg taatggtcat
     1501 attctatttc atttattaat cctacgcaag gacctttaca tttttttaga tgatactcta
     1561 ggcatacttt ataatttcca ttctttattt ttttctcgga taaatttaac ttacaagtgc
     1621 gtaatggaaa aattttgtgc atgagttcta ataaagattt agctgttctc actgaagcat
     1681 agggaccgta gtattctgca ttatgaatgt gcctatttct agttaaaaaa atacgcggga
     1741 aaggctcatc agtaatagca ataaatggat agcttttgtc gtcttttaat tgcatattgt
     1801 accgaggctt tagatttttg atcatattat tttcaagtag tagtgcctct agttcagtat
     1861 tagtaacaat agtctcgatt cgttcgattt ttttaaccat tattcgtagt ctagcactat
     1921 tatgttgtgt gcgaaagtat gatgagactc gactttttaa attttttgcc ttgcctacat
     1981 ataaaatatt atcatcttta tcgtaaaatc tataaacacc aggacttttg ggtaaagttt
     2041 ttaatattat agatatttgc ccttcattca ttttttagaa attatttttc taattttatt
     2101 tatttgtttt atataattct cgcaatttag catatttcaa aaaaacacca taagctgaga
     2161 tcattgatat ttgaaagcct tcgtatccat ctaaaaaacc agcatgataa atataatgat
     2221 taataaattt atgaagtggt tttataaaaa aatgccaaaa atttggtttt attttatatt
     2281 tatagagctc attagctgat atttgtgtga atttttctaa ttgatttaaa tgatcattta
     2341 gagtaaaata tgagtagtgg agtatatcag catttatatg tttaatatta ttactattgg
     2401 tgagctcaag tctatcatga ggatttatgc cgcaccattg agctaaatct ttgcgtacta
     2461 gtctgagttt tttgtctgga taccatcctg tatgcctaat ccatctacca cagtagtatg
     2521 tgagtctatt catggtatat ccgtcagcag taggattttt tttaatttct tggattttgt
     2581 tttttaattt atctgacaat gcctcatctg catctagtga taatacccat gtgtagttag
     2641 ccttttgaag tgcaatattt ttctgttcta tatgaccttc aaatttattt tctatcacaa
     2701 tagcacctaa atctagagct atatctttag ttttatcggt agaataagaa tctactacta
     2761 ttatttcatc ggctatttct ttgacagaat taatacaccg tgcaatattt ttctcttcat
     2821 taaatgtaat aataactata gatagtttat tttcattttt atgcataata aacaaaataa
     2881 aaaaatactc aaaaaaaatt gatttacaaa tttaataaat gtttgataat taggtgtatt
     2941 catttacaac atttttgata attaaaaaaa aaattaatct ttacgtagta atataaaaaa
     3001 ccttaaaaaa atcagttata tttttcttgt taattaattt aagttgttta tatttgtttt
     3061 ttgggtctta gtctataaaa ttaaaaataa attgaagcaa tagtaaaaac acctattatt
     3121 ataaataaca atccagaaac aattgatagt gtcttggcct ttacatactt cattaaaata
     3181 tttccaagga aaatagacaa aatagtcata acagaaatac cgataatagt acctaaaaaa
     3241 actaaaggtg gattatactg tgtagctaag gtagctgcgg ctatctgagt tttatcaccc
     3301 cattcactta aaaatattaa taaaaaacca gttataaaag gattaatatt agttttagta
     3361 aagttaattt ccttttcata tcttaccaaa gttattatac caaaaatcaa aaaaatcaaa
     3421 ccagctatta ttgtcattat atttttaggt attaattcag ctattaagcc tcccgcaagt
     3481 atggctaatc catctactat agcaaaagcc aataaaacac caaaaaataa agcaaagtat
     3541 ttttttgttt ttgatgctat tacaattata agtaattgag ttttatcagc aaattcagca
     3601 accccgatta gtaaaaatgg aataataaaa tctctaaaca tttgtatggc tattttttaa
     3661 ttttttttgt aaaattaatt taaagaaatg atattttata aattaattta taaataaata
     3721 gtaaaaatat ttttatatta ctaactcata aatttaaact atatttgcta aaaattaaat
     3781 aattatgaaa ataaatcttg taactggagc atgtggattt gttggtagaa atatggttaa
     3841 aaggttgtat agtaccacca ctgatcatgt ttttatggtt gatgacctta gcattggcat
     3901 tcatccaagt gaatggttcc caaattatta tccatctgat tataatggtt tagaagtttt
     3961 agatcctgac aaaagagttt atttttggaa gggagattta cgagaattac tatttaattt
     4021 tcgtgaaaat cccagatata ttcaggaaac ttataaatta gattttgata aattttctga
     4081 tgtatttcat tttgctgcta ttgtaggtgg acgcttaaag atagatgggg accctgtaca
     4141 agtagggtta gatctatcaa tagatgcaga gttattttac tgggcggtac gcaatatgcc
     4201 tgatagattg ctttatccta gtagtagtgc agcatatcct gtagatttac aaactgagga
     4261 gacggcctta gcactaaaag aagaatatat tgatttcgat tctttgcgta tcggtgtacc
     4321 agacatgacc tatggatggt caaagctaac aggagaattt ttagcaaaga tagcagctaa
     4381 acattatgga ctcaaagtag tgtgtattag accatttagt ggttatggag aagatcagga
     4441 tctcagttat ccagtgccag cgttagctgc tcgtgccgct gccaaagaag atcctttcgt
     4501 agtatggggc acaggcaagc aagctcgaga ttttatccat atagatgata ttatcgattt
     4561 tatctttgtt ttaatggata atgtgcatga cggctcagga tggaatattg gttctggtaa
     4621 attaacatca tttttagaaa ttattgattt atttactaaa tttgctggtt ataagcctac
     4681 tattaagcca cttatagata aacctatggg agtgcattct cgctatgctg atatgactaa
     4741 ggtgttttct acattcgatt ggcgtccaaa agttagttta gaagagggaa tgtatagagt
     4801 atataaagct caattagaga aattaaataa aaataaataa tttttatgcg gataatttgt
     4861 tttgggcctg gccctcaatt taaaggtggg cttgctaatt acaacacttc tcttgctaaa
     4921 gcttttagtg agtttgacaa ggcagaagtg catatagtat cttggactaa tcaatatcct
     4981 agcattgtac ctcgcgattt cattgatagg aaaagcaggt ataatgcact cgaaggtact
     5041 gatatcaaag tgcattatct gactaattat aataatcctt ttagttggat taaaacggct
     5101 aaacttatta aagatattaa tcctgatatt gctatctttc aatggtctat agctatgcaa
     5161 gggttacctc tcagatatat cgttacttgg ctaaaaaaac attctaatat tcatattatc
     5221 tttgatttac attttgtagt acagaaagaa agtagctcct tagataaatt ttttacaaaa
     5281 ttcgccctta agcatgtccc tcattttatt gtacattcta aaaaaacttt tgatgaactt
     5341 tgtgatgttt tacctaaaag gcagtttcag ttagtacaag agtttaatcc aaaaaaaagt
     5401 gattcacaag tgatacacct ctatcaccct atctatgata tgtttaagcc aaacgaagat
     5461 ttcgatgtag aaactttcaa aaaagaacta ggattgcaaa agtatgtttt tctatttttt
     5521 ggatttatcc gcaaatataa aggtttacat aatgctataa aagcctttgc agaattatca
     5581 aaagatagag acgatgtatc tttattgata gtaggtgaag ctttttggtc taccctagac
     5641 aagaaaaaat ggactacacg ggttaaaaaa tggctcttta atgttgcaca aagtattttt
     5701 agaaaagata ctgaaagtga agataattat cgtcccttag atttagtaaa agagcttggt
     5761 atagaggata aagtattatc tttgatagaa tatgtgccta atgaggaggt atataaatat
     5821 tttcaggcta gtgatgctat agttttgttt tatgaatatg ctactccatc aggggtggaa
     5881 tctatggcat ataattttaa aatcccaata atagctactg cagttggaca ttttccagaa
     5941 actataataa atggcgttaa tggttatcta gcagatcctg atgatatcaa atctatggct
     6001 gaagctatga aaaaaattat tgaaaaacct atccatagag caaatatcga aaaaatagct
     6061 aatgaaaaat cttggcatgc atatgctgaa gctattgtaa aaaatattta taccaacttt
     6121 acaaataagt aaaatataaa ttttcaaaca cagtcattag ataaaaataa agacacattt
     6181 tttttaaaaa aatgtgtctt tatttttaat tgttgatttt aaatactaaa ttattatccg
     6241 agataggctt ttaaattttt agatctatta ccatttttaa gttttttaat tgccttttct
     6301 tttatttggc gtaccctttc acgagtgagt ttatatttat ctgctatttc atctagagaa
     6361 taggggtgtg cataaccaat accatagtac atatttatga tatctctttc ttttttagta
     6421 aggctagaca atgcacgttg aatttctttt gataagcttt cttgcatcaa tgaatgatca
     6481 gtagaagggg catcactagg aacgaaaaga tctaacatag tagcatcttc atctggtgat
     6541 ataggggcat ccacagatac atgtttgcca cttattctga tagattcggc aactttagat
     6601 tcgggtatgt ctactaaatc tgctatttct tcaaccgatg gggcacgctc aagctcttgt
     6661 tctaatttag cagattcttt agaaattttg ttaatagaac ctatttgatt gagaggcagt
     6721 ctgactatgc gactctgttc tgcaatggct tgcaagatag attgacgtat ccaccataca
     6781 gcataagaaa taaatttgaa accacgcgtt tcatcgaagc gttctgctgc ttttattaag
     6841 cctatattac cttcatttat taaatcttgt agacttaatc cttgattttg atattgctta
     6901 gctacgctaa ctacgaaacg caaatttgcc ttaactaatt tgtttcttgc tgcttcatca
     6961 ccttgtttaa tgcgctgagc taatttaacc tcttcctcag gagttattag ctcttctcta
     7021 cctatctcct gcaaataatc atccaatgag gtataatccc tattggtgat cgaacgtgta
     7081 atttttaatt gtctcatatg ttttaatacc tcttttttat gtattttctc atgtattttt
     7141 ttttaattaa taatttatac tataaaaaag tatacaaaat tacaaaattt ttttgatatg
     7201 gcaaaatatt tatgtagcaa cattataatg taaacgcata ataagctcga attccattag
     7261 gatatacacc ttctatcagt actgctccct ttctattttt taaagctttt tcaatatctt
     7321 ccacagaatt gataggttta tggtctattt cagtaataat aaaatctttt ttgattcccg
     7381 ctctttgaag tggtccccct tctatattta ttatttgcag accatgatct aggttgagtt
     7441 tggccattaa atctggagaa ggtttttcga aactagcacc taagatagct tgcactgtca
     7501 tatcttctcg tttcactggt ttagaatcac catatttatt taatagttga atttttgctg
     7561 ttttcacttt atttcctgtt ttatattcta cagtaatcca gtctccgata tttttttcat
     7621 taattttttc tactaatcta gaagcagaat taatctcttc tccatcgagt ttcaaaataa
     7681 catcaccagc atctaaacca gccttaaaag cagcgctatt aggagtaacg gccgttatca
     7741 aaacaccttt tatctcatca atgttatatt ttttagctat ttcggaattt atatcagtta
     7801 tgctaacgcc taaataaggt ctttgcacaa aaccatagtt gataatatca ttagcaactt
     7861 ttttagctaa attagatggt atggcaaaag agtatccgat aaaggcaccg gttttagttg
     7921 ctattgctgt attgatacct attagattgc cgtatatatc tactaatgca ccaccactat
     7981 taccaggatt tactgctgca tcagtttgta taaaagattc tactgcatct gcagattcta
     8041 atatattaat atttctagct ttagcactta ctataccagc tgttactgtt gatgtaagat
     8101 taaaaggatt gccaactgct aaaacccatt ctcctatttc tatatcatca ctattaccaa
     8161 attccatata tggtaggttt ttagcatcta tttttattat agctagatcg gtgctaggat
     8221 cattacctat tattttagct tgaaattctc tattgtcatt tagtgtaact ataattatat
     8281 tagcatcttg cactacgtga ttatttgtaa ttatatatcc ttcgggactt attattatac
     8341 ctgagccccc tccgattatt gtttgctcac gactaggacc accaaagaat ttaaaaaaat
     8401 cataaaaagg agcaaaaaaa tcatcataat aaatattttt aaaagtaaaa gtagttttga
     8461 tatttactac agcaggtata gcttttttgg cagcatatct gaaatctgga aatttgatag
     8521 tatcacctat gatagcatat ttttcataat tataattgtt tctgttttca ctcgctttac
     8581 taggactagc tgtaaagcta tatataacta taaaaaatag tactaatgtg caaacactca
     8641 ttactttttg aaagtaattc caaaattttt ctactttttc ttttttcata attttattaa
     8701 tttttatttc tttattaata tcatcaaata ttattccatt tttttattaa ttaatttttt
     8761 atttttattt tcattttaaa aaaaagatgt acatttgtaa atgtcaaaat ggcatatatg
     8821 aataaaaagg gatacttttt tgttattggt attataggtg gcatcatatt atgtatcatt
     8881 ttaatatatg cttttttgcc taggtttaaa aatttggttt atcaacctga atttttttat
     8941 tattctgata tcattgatga atacgaaata aataaaaaaa gatcttttga tatgatggag
     9001 tctcgtttta gaaaattata caaaagtaat ttcaaattat taattaatga ctcaattaat
     9061 aataataaca atgatagttt aactagtgca aaaatattga atattaaaaa taaggatacg
     9121 atttttaaac ttttatatta caatattttg gattcactcc ttaaaatatc aggatataaa
     9181 tatgtagatt ctattacttt agattcatta gtaaaggtaa tgatattaga ttcaatagaa
     9241 aaaataaaaa tcgatagcac aaaagtattt ttttaatgaa caatgaaaat ttttttatta
     9301 aaaataattt tcatataaaa tatttaatgt aaatttgaag tcaaaaattt ttaattatga
     9361 aatttattgt atcaaatgcg gaattaagca aagcaattaa tagtattagc tcagtaccta
     9421 ttgcaaataa tgttattaag attttagaaa attttttgtt caaggtgaac aatagtaaat
     9481 taactatcac ggctacagat ggtgatacca gagctagtgt aactatagat ctcattgaga
     9541 gtgatggaga aggcgaaata gtgatgccat caaaattgct tcaagagtac ttgaaaaatg
     9601 taccttatat ccctattgcg tttaatatgg atacaaccgg tgaaaataat tatcaaataa
     9661 ctatttcagc gggtagtgga gtatatcgta tggtaggaat ggatgcagaa aactatccta
     9721 caatgcctca aatacccgat gctaggactg tgaaattgaa tagtactgta ctgtctacag
     9781 ctttagaata tactttattt tgtgcagctg tggtagatta tagacagata ctcacaggtg
     9841 taaattttga ttttcgtgat gaaaaactaa atgtagtgtc tacagatagt catagattgg
     9901 ttcgttatcg cagatttaac attgaaactg aggaagattt taattttgta gttaaaagaa
     9961 aaccattaca aatactcaga aatttaattg atacagacac agatgtagag attaacttta
    10021 acgaaactaa tgtatctttc gaatttgaaa acataatgat atcttgcaaa ttattagaag
    10081 gtgtattccc agactatgat agagtgatac cacctacatt tgaaaacaaa ttattaatag
    10141 ataaagaatc ttttatctcc gctctgaaac gtgtagctgt atttactgac aaagctacca
    10201 accaaataag gatgcatata gaagggcaag aaattacttt atctggcaat gatgaggaat
    10261 atgctaatga aggtatcgaa aagctccctt gtacctacga aggccaagat atggatatat
    10321 cttttaatag taaaaccttt atggaaatgc tacaacattt agattgtgat aatatatata
    10381 tccttatgaa taaacctaat actgctatcg tgataacacc tgcagaagaa aacgaatcag
    10441 aagatatagt aatgttgcat atgcctattt cattaaaagg aagtgaagat tattattaaa
    10501 cctttttttt ttgacatatt aaaaatttta agaaatctta tatatttttt tgtataaaac
    10561 tattaaggta aaagattgaa tttttcacaa attttcatcc actttactag ctctatattt
    10621 attagtcaag tcattgtatg tatttaataa aatggcttcg atatttttag ttgcatattg
    10681 tgtatcgcca ttataattat ttacaatagt cacaaaagct attgtatcca taccatcggg
    10741 tagatcaaaa tatccagcaa aagctctagt attgcgcaaa taaccacttt tagctcttaa
    10801 attattctca agaggggttc ccttcaaaaa ttttttcaaa gtaccattga taccagctac
    10861 tggcaaggaa ttatataaag aatgaaacca attagaatta taagatgcta ttaaaagtgt
    10921 caaaactgca tcagcagtca atttattttt tctacttagt ccactaccat cacagatatt
    10981 tatatccgtg gtatcggagc cgaatttata aataagttgt ttagccaatg aaccatagga
    11041 ttcataagat ccatcactaa gtttcttagc aatagctttt gccaaaattt ctgcacctac
    11101 attataacta tgagtcagtg taaaagaaat aatactatct aaaggcatag atttatattc
    11161 gaaaattaat tttttagact cataagatat agtatctgta tatgaagctt tgagtgttcc
    11221 gattagaaag atatcattta ttaataaagt attttgagtt ttccaagcaa aaccataagc
    11281 tgggtctggg ctagatgctt tcacaaagag agtttctcct ttaccccttg ggattgtacc
    11341 agagaaaata taattttttt ctaaaggttt gccagagaca tgagtgtaat taccatagga
    11401 tctattagct acttttactc tattttgata attgacatga gggaaaactg gttctgtata
    11461 tattaattta gcggttttac catattcttg gtttaactta taagctattt tccaagtatt
    11521 accattatac ataagagaag atgctcctgc accataataa ttgccaatat cctcatattc
    11581 ataagtttta ggaattatct catcaccaaa atagcttgca tctgctatga tattgccata
    11641 taaagtatct atgcccattt gctttaaaat attaactaat gaatcaaaga atttattgcc
    11701 ttcaggaaaa tcttctccaa tcaaaggatc gccagttccg aataaatata ggtttccatg
    11761 ccacacatta tttttttgat aaccagaaag atatgcattt gtagtaaatc taaagctatc
    11821 acccaaataa taatatgaag atatggcggt taccaattta ttaactgatg ctggtattaa
    11881 cggtttatat tgatttatag acaaaagagt ttcagatttt gataagctat aaagtataaa
    11941 tgaatatgaa tttttatcaa ctctgtgatc attatttaat ggagataata taaatgattc
    12001 atttagggat tgtgcagata aattgaatac acttaataaa gcaaaaataa tgataagttt
    12061 tttcatttgt atatagtttt tttttaaaaa agtttgataa ttaatgtcaa atttaagaaa
    12121 aaatattcat agtaatatga tttttatctt atagatattt cataatatta acatttctat
    12181 taaaaatcaa ttattttaat aaatttttaa taacaaattg cacccatgga tctttaatgg
    12241 gatcattaaa attgggttga gcccattcta aagggtaatg aatatctatc ggttctctat
    12301 catattctgg aaattgtaca aactctaata agctcaagga gacagaaata tgagaatgtg
    12361 gtaataaaaa tattttactt tgtccgaaag tacctttttt agtccctcct gggggcaatc
    12421 caactaaata agtattagga cgatgggcca aaagattagc agctagagaa gatgcagata
    12481 tactaccacc atcagttaat acataaatag taccattaaa atgatttttg tgtggtttag
    12541 tttctttgtt tatttcatat actgatgggc ctttataacc agcttttaat aatttaagct
    12601 ctttattttt aatagatttt aatgttaaaa taaaattata ccattctttt tgcaatagtg
    12661 aattttttaa taataattga tcagtccatt tactgggatt tactctatat gttttgacat
    12721 atgtaaaagg tgcaggcatt aagtatttca aaaaatcttc tgctactttc acataaccac
    12781 cgggattgcc tcgtagatct aaaataaggt acgaaatatt atatttattc aaattcgaaa
    12841 tcactatagg catgagttga tcgggagtat tatatttatt atttaaaaaa tcagaaacat
    12901 ataaatagcc catattgata gtaggatata gtttaaattt atacggattt gataaatctt
    12961 ctatatatgg agcaaaaaag gattgtgagc ttttgatatc acgcatagag ataccatcta
    13021 ttatatattt tattgtatca ttatttattt catcgagcaa aacacaatta tttatgtttt
    13081 taacttgata aatgaatggg aaataataat agaataacaa tgaagcatta ttgtcttttg
    13141 tggttcgcaa acctaaatct gcattgcata gattagctaa ggtactataa atttcgctag
    13201 catctatatc attaatagat aaaatacgag tattattaaa aaatttatta ttagaaacac
    13261 cttttaataa ataaatcttg ccatcattaa atgccactac accgccaaaa aatttaggaa
    13321 aaactttttg tgcagcataa taatttaaat ttaacattag atgggaatca tcaattttag
    13381 aaaagatata tgctaatctt acaaaaaatt cgttgattga gacactatca tcgatagaaa
    13441 aaaataaatt agcaaaagca tttttcaaaa aaacagtatc agctacttcg ctaatattag
    13501 gatgtgcgtt gcataaatat tgatataaat gagctagatc tatatacgct tcatgcttag
    13561 acattttata gtctttataa tcctgagctg tagcatccca actaataaaa atcaaaaata
    13621 atgaaaatat cttaataaat gatttcattc tttttttgca aatatattaa attcaaataa
    13681 caaactaata attttaaaat aacttataaa tcagatcctt tataataaat caagggttaa
    13741 taatcaaatt aggaaagatt taaagataga gtgggagaca ttttttatat tgttaattta
    13801 ctattttagt atatttcaag ttaagattat tcataggatt tgtatttagc cctttaacgt
    13861 atggctgcaa aagcctgatc gaaacatcgt agtataatgg tactactggc atctcttcta
    13921 tcaagatttt attcatttgt ttaataattt catatttatc agcattagat gttttcagta
    13981 gactgagata taattcatca aaagtattat tcttgaaatg agtatagtta ggtcctttag
    14041 gaggtatatt attactaaaa aaaatcgcaa aataattttc tgcgtcggga taatcagcta
    14101 tccagctacc tctgaaaaac tgagcttgtc ctttaaacat tagatctcgc aatgtggcgg
    14161 gaggcattaa ttctatacta atatctaatc ctattttact taattgattt tgtatatatt
    14221 ctgctatgtc tatatatgta tcattagtta gtagggtgat tttaggttta ttaggcatat
    14281 ccatataacc tgaggcaata agtaattgtt tagcgtagtg agggtcatag ttcaaatatt
    14341 tattagagct atctaatgga tttaatatag gaggaatgaa tccatataat gcaggatatc
    14401 cgagattgtt tctcagatat ttcatcatag atattctatc tattcctaga gatatagctt
    14461 ttcttaattc tttattatta aaaggtgtat catcactaat caaaaaaccg agatattcag
    14521 tattcaagaa aatacttttc tgaaaaatat atttgttttt aaactctggc tttaattcgc
    14581 cattatcagt aaataatata tctttaaacg atggatctat tccggataag aaatcaaaat
    14641 cgcccagaag gaaatccata aaagctactt gtttatcttt gataaatgat atagacactc
    14701 catctaggta tggtaattta ttgcccaagc tatcaaactc aaagtaattt tcatttttag
    14761 cgagtaataa tttcacatct tttttccaat atttaaaaac aaaaggtcct gtgcctatag
    14821 ggtgatgcga aaaactttcg ccatacattt gtatagcttc tctgggtata atactacaat
    14881 atggcattgc taagatagac aaaaatgaag taaaaggttt tttcagataa atcacaacag
    14941 tagagtcgtt tatagctttt acgctcaaat tattattatt tgtagatata gggctcatta
    15001 cccatctacc tggtgaggca gttttttcgt cagctagtct ttgtaaacta taaactacgt
    15061 cgtcggctat taatttacga gtttcattct caaagcattt atcattatga aaaaaaacat
    15121 tgtctcgtaa gtgaaatata tatcgacatc catcatgtga tatttcccaa cttttagcta
    15181 agctaggtaa taaatttaat gaatcatcga agctcactaa tgtattaaaa agctgttgta
    15241 cgacccagat atgtgcctga tcgcgagcaa aggctggatc taaagaatat aatcctgcag
    15301 gctgattgta gtggaatatt tgtttattat tatttatttc tgttttataa tttttattac
    15361 agctaaaatt tataaaattt agtagtatca aaaaataaat tagtttgtta ttagtaattt
    15421 ttattataaa taaaaaaaat ttattctttg agattgatgt tctcaatttc atttaatttt
    15481 tgaaatttta tatgcaaaat aacataataa atcaataaaa ttcactcgat acaatattaa
    15541 taaattatag tgcagttagg ccagataaaa tatcataaaa taataatata ggaactaact
    15601 tagttaaatt cttgtagagc agctttattt actttacctg aacctgtttt agggagttca
    15661 tttaaaataa ttatatcctt tggtagttta aatttagcta gattatttct caaatattcc
    15721 ataagctcat ttttatcaag atctatgcta tctactggag ctatgtaaca tttaactacc
    15781 tcgcctctta attcatctgg tacacctata gccgcggctt ctctgatacc aggatagcga
    15841 agcagtactt cttctacctc tgctgcccat actaactgtc cgcctacttt gatagtatct
    15901 tttgatttgc caatgatata gtaattacct tcttcatctt ttctagccaa gtcgccagta
    15961 tagaaccatc catctttgaa aacttcattg ttcaattcag gctcattata ataaccacaa
    16021 aaaactgatt taccacgtac tatcagttca cccacctcac ctacttttac ttcattacca
    16081 tcaggatcta ctatttttag ttctacatta ggcactggct tacctacgga acgaatattg
    16141 ctaggtgaag aagcgcatac aggaggtatc acctctgtca tcccccaacc atttattacg
    16201 atggcgttag gacataactt ttcaaatttt cttattaaat ctggtgacga tggagcaccg
    16261 aatactgtag cccaacgtaa tgaattgagt gaaaatttat caatatcttt taaatataaa
    16321 acagcataat acataggtgg caccataaat acgccagtta cttgccattt atctattaat
    16381 tttacaagtt gcaaaggatg gaattgaggc attattacag aactaacgcc atatttgata
    16441 gctgtcatag ggaaaaatat accagcagaa tgagaaaaag gtatagctgc tattattctc
    16501 tcattttcat caaaattaat tattcctttc aattcttcat tagtggtaaa aaattttatt
    16561 tgctctgctg tcaattgtat atgttcataa ttccataatg cccctttggg tctacccgtt
    16621 acaccggaag tataaaagat actcgatatc atatctgagg ttacattctg atcattcaaa
    16681 ttattatcat gagtatttat aagatcccaa aagtaaatat aattggtttt gtcttcgata
    16741 gttataatat tttgtaaagt aggtatacta tctttgagct ctgataaatc aaatttagct
    16801 ttagatgtag taatgatggc gctaaggtca caatgtttag ctatagatat taattcatta
    16861 gcactcaaat aaaaatctaa tggtatagca atgcatccaa gtgaataaat agcataatat
    16921 gaatagacat attctatgca gttaggcaaa taaattgcta ttttatctcc tttttttaat
    16981 ccaaggtcta gaagtgcatt agctagtcta aatgaatcat tttttaaagt attaaaatca
    17041 aactctttgt cattataaat aaatgcagtt ctgtcattat actgctcagc tgcatctatt
    17101 atcaattgtc taatatcttt tcttttttcc ataataattt attttttata atttaagagt
    17161 aatcataaat tatattttgc atacaaatat agtttatttt tttttaaaaa tagtaaatat
    17221 ttatgaataa tgtcaaaatt tattttggta ttattggttc tataacgttt attgtgggta
    17281 aaaacatttt agattgttac aaaaaataac tattttttaa ttatttaggt tattttttaa
    17341 ccacaaagtg ctatttatag cctttttaat tccatctttc tctgcgttca ttgtgcattc
    17401 tttgagaact ttgtggttga tatttaactg tccaataata aataaaccaa tattaacaaa
    17461 tttattttgg tattattatt taatattgct ctaggatttt aattttttaa gcacgataaa
    17521 cacataagca aataaaaaat acaaatacat taatacaaac ataataatat gtggggatgt
    17581 caataagctt cgcatattaa aaacagttaa atacaaaaca aaaaatatca aaactattaa
    17641 taatctagct atatctatta ctaaataaag gataggtttt tttttcaatt tattatcttt
    17701 gtaatgaata aaaattgtta ttgacaaaaa gatcaaaaag ttaatagacc aaaataaaat
    17761 tatagaaata tcaataggtt taaagatcaa ccataaaata ggcaacaaca aactaaatat
    17821 agaaaagata ctaaaatatt tccacattat ttatttttta ataattctcg tataccgata
    17881 taaattgcta ttaatgtacc aatggcacct aatatactag tatatagata atttgcatta
    17941 tttttttcat caagccattt acctaaatac actccaccta aaataattac taacatttgt
    18001 atagcaagat tactgtactt aatatattta tctaaattat tttttttcac tataaaaaat
    18061 aattattcag attttacttt catagtacat ttgccattaa atagggctcc agcttgtatt
    18121 gataatttaa atacattgat atcaccagtt agctcactgg attcttttag tatgaggata
    18181 tcataagctt ctatattacc tgttactttt ccatttacct ctatattttg tgcttttata
    18241 ttgccattta tagtagctgt aggtcctatg ataattctgg aattagaatt tatattacca
    18301 tttactatac catcaattct aagatcatct tcagttgcca gatcaccctc tattctagtg
    18361 gattctccta ttaaatttac tttagtcatc tctacttctg gtatattctt tgccataatt
    18421 tttatttttt tttaaaacaa ctgcaaatat aagattattt tttatacaaa aaaatttttt
    18481 tcaaggtaca attatacatt tcccaaacta cctaaaacta attgtttcag agaaattata
    18541 ccagtgagat ctatgaagat aagaatcatt atagctacga tgagccattt aacaaaattt
    18601 atacttttgg ttagagaaat tctagctgct atctgagcac caataatgtt gcctataccg
    18661 tgtaccagcc catattccca atttactagt ccattaatta taaatataat gaaagatata
    18721 gagacataga aaaatactaa aaaattttta aaagcacttg cttttatcac atcatatccc
    18781 attagataaa taccagcaaa taggatataa tatccgacac ctacgtgtat aaatccacca
    18841 tatagaccta ttataaagaa aactataata gtaagccatt cgtttctttt gaaatttgca
    18901 ttattttctt ttcctacggt ccagctctta ggattgataa acatagatac gatcattatt
    18961 actataacta ccatcagagc ataacgaaaa aaatcttctt taacattaac agcaaaaact
    19021 gcccctatta atgaaccgat gacaatggcg agagcttgtc gccaaatatt tttgtatggt
    19081 attactttat ttttaaaaaa aactgcatta gatgatagag tttgcattat tatggcgact
    19141 ctatttgtac catttgcaat agttagtggt aggcctaacc aattaaacac tgatatggaa
    19201 ataattgtag caccaccagc taaggtgtta ataaaaccta ctattaaacc agaaataata
    19261 agtatgatat acaatttcat ttttataata agttaatttt aaaatttaag gtgtaaataa
    19321 aaaagttatt tttttggttt tctgatcaaa atctgcccaa gtatcgctaa atatttctaa
    19381 acctaacaat gaagctgttt tcattttatt tatactatag tcagtgagca tagcagctag
    19441 catggctatg actggattgt gtcctactag cattaatttt ttaacattat tgtccacttt
    19501 atatatttga taaaaataag catctatatc gccataatac aactcatcat tagtgataat
    19561 aatattatta gataaattta aaatctcaga tacaatctta gctgtttgca gagctctaga
    19621 tgcagaacta gtaattatta attgtggaat aaaattaata gtaacaagat attctgcact
    19681 aagttttgct tgcttttcgc cttcctcact taattttcgt tcacgatcat tagtatgaaa
    19741 attatgaggg agagctttag catgtcgcat taggactaga taacgtttgc tattaccagg
    19801 catacaaagg aaaatctttc attaatttgt ttacctcaga ttttactttg tttatattat
    19861 tttcattttc tatatcattc agtactctat ctattaggtc tacaatgata ggcatgtgat
    19921 tttcttttaa tccacgagta gtaatagcag gggtacctat tctaatacca gaagtttgga
    19981 atggagaacg tgaatcaaaa ggtaccatat ttttattaat tgtaatatca gcatttacta
    20041 gagtattttc tgcttgccta ccagtaagtt caggaaattt tgatctaagg tcaatgagca
    20101 taaggtggtt gtcagtgcca ccactaatga catgataacc tttgtctaca aatgcactag
    20161 ccatagcttg agcatttttc attacttgat taatgtattc tttgtatgaa ggttgtagat
    20221 cttcgtaaaa agcaacagct ttagcggcta ttacatgttc taatggtccg ccctgtgttc
    20281 caggaaatac ggcgctattg atcagctcag acatcatttt tactttgcct ttgggtgtag
    20341 taatacccca tggattttcg aagtcttttc ctattaaaat catgccacca cgaggtccac
    20401 gaagtgtttt gtgagtagta gtagtaacaa tgtgacaata tttgagagga tcgtttaaat
    20461 atttgcaagc tattaagcca gcaggatgag caatatcagc cattaatata gcacctatgc
    20521 tatcagctat ctgacgcata cgtttgtaat cccaatctcg actatatgca gaagctcctg
    20581 ctatgatcat tttaggtttt tcttgtatag ctattttctc catttcatca taatctacat
    20641 atccatcttc atttacatga tatgcaacag gattataaat aataccacta aaattgacgg
    20701 gcgagccatg cgtaaggtgg ccaccgtgag ataaatctaa tcccataaat ttatctcctg
    20761 gctttaaaca agtaaaaaaa actgccatat tagcctgagc accactatga ggttgtacat
    20821 tagcatattc tgcaccgaat agttctttag ctctatctat tgctaattgc tctgtctggt
    20881 ctactatttg gcaaccacca taatagcgtt tttgtgggta gccttctgca tatttattgg
    20941 ttaatactga acccatcgct tcgagtactt gggaactaac aaaattttca ctagctatta
    21001 attctattcc ttctgtttgt ctttgaaatt cttgttcaat taatgaaaaa atttttacat
    21061 ctcttgtcat aacttttttg caaatttaac aaaaattaaa attatgaaat tataaattta
    21121 gtatattttt gcattataat tacaattatg atttggaaat tagtagaaaa aattgaagct
    21181 aaagctacac aatggtatca ccaaatacat aataatccag aattgagtgg cgaagaggta
    21241 gaaactgcta aatatattac taaacttctt agaagttggg gaataagtgt taggactggt
    21301 gtagctggta atggtgttgt cggtataata gaaggcaaag aagtcaatgg taaagttata
    21361 ggtttaaggg cagatatgga tgcactgcct atagacgaaa aaacaaattt accatataaa
    21421 agtaaaaaac caggtattat gcacgcttgc ggtcatgatg ttcatgtagc caatttatta
    21481 gctagtgcat atatattaaa cgaaatccga gaaaaatgga atggtagggt ggtattaata
    21541 tttcaaccat cagaagagac attgccaggc ggagcactca aaatgataga agctaatgta
    21601 ttagatgatc catatgtaga tgtagtaata ggcatgcatg tatcgccaga gctgagtact
    21661 ggtacaatag gatataaatc aggagttttc atgtcttcta cagatgagat ttatatagaa
    21721 gtaaaaggta aaagtggaca tgctgcgtta gttaaagaat ttatagaccc agtggtatca
    21781 tcagctgcta ttataatcaa cctacaacag ataataagca gaaggaataa tcccatcaca
    21841 cctacggtat tgtccattgg taaagtgata gctaatggta gaacaaatgt gataccgtca
    21901 ttagtaaata tggaaggtac atttagaaca tttgatgaaa attggagaaa tgaagctaag
    21961 acactaataa atgaaattgt ttataacaca gctcaagcat acgccactac tgcaaatgtt
    22021 aatatactat ctgggtatcc gaccctatat aatgatgaaa aactaagtgc tgaaataata
    22081 aattttataa aaaataattt aagtgagatc aagctcatcg aagtacctat gcgtatgaca
    22141 agtgatgatt ttgcttattt tgcactccaa cgtccttctt ttttctttag gctaggtact
    22201 ggaaatgaaa aaaaaggtac tacttatcag cttcatactg ataaatttag aatagacgat
    22261 gaagcactga aatatggtat ctatactatg agttcgattg cttttggatt tttaaataat
    22321 caaataaata tataaatgaa aaataaatat ttatttataa aattatttaa acttgttttt
    22381 attttgatat taatgagtca gatattatat tcatgtaaaa acaaaaataa aatcatattt
    22441 tcaaaagagg tagtattcat taataatcta tgggaaaaag atcatacgct cagtttttta
    22501 tttcaaatca cagatacttc tgctaaatat gatgcattaa taaaggtaat gttagatact
    22561 aattatacct ttaatacatt tgtttattcg ataaacttaa taacaccaga tggatcattg
    22621 aggcaatttg gctttcagac agatataaaa gattatgata ataatataga gctacctatc
    22681 atcggtgata gttggctaat agataaaaag gtgtttgaag atataaaatt taataaatat
    22741 ggccaataca aaatagaact aaaacataat atgcagttag acttattttt tgtaaaatca
    22801 gtagagttga taataagtaa aaaataatca agtatcttcc caataaaaga ctccatcttt
    22861 atagaccata gtatccattg ctaagcaaga atgtactggt ataatagcta tatgatcgcc
    22921 aattttaaat ttattaataa attctttatt aatagaaatg acaccatgct cttgggaaat
    22981 acttttaaca taggcattag gtatcaaagt taattttttt aaatcccaag tagctactaa
    23041 gccaaacttc atgataccct catctatcaa aaaatctttt gatagatgag cagcaccacc
    23101 gtgcattatg atctcttcac gttctggatg aatagatatt atcggtgcat acaaaaatat
    23161 agctatgtct tctaatgagc aattgccatg caaatattgc attgcatcgt aaaagacaaa
    23221 attaccaggt ctgatttcat ctactttttc gagtaatttt ttattgtata tcctcaatga
    23281 tggagtgtcg ccaatggaca aggatatgga tttatcgagc atggcttcta ttattctttt
    23341 aatatttgat aatagttcga tatttgttgc atttaactca tatagttgat catctgtaga
    23401 taatttatat gattgtccga aatgagaaaa aataccataa aatttattgg ctaaattatt
    23461 actaataatt tcattgataa tactatagat ctcatcttct tcgctaactg atatgccact
    23521 acgatgatag ccacaatcta tttcaatagc aaaatttaat tttgtatttt cttctaaata
    23581 aggtatcaaa tatctaaaag cttcgataga atcaatcgaa actatcaatc tattcaaata
    23641 ttcatcatcc aaaacataat caatcctatt gagactatgt atatttatgg gaatagcgat
    23701 aaatatatca tttatttttt catttataaa tctgtaagcc atatcaatag atgatacagt
    23761 aatagcattg ataccgaaat tttcaaaaat aaatgatatt acatctgatt gatgcgtttt
    23821 gaaatgtggt ctgagtgata cctcccattc ttgtgtttta ttaacgattt tattgatatt
    23881 gttttttata attttattat caataattaa ttttggtgta taagaaaata aaaaattttg
    23941 ttctttcatt tttataatcc gattttattt gtaaaagcca aaattaaatt atttttttga
    24001 taaagatttt ttcagtttat actttgaatt agttatatta taaataattc tactaaaatt
    24061 ttatccatta tcaaccagcc acgctcggtc agataatatc tgttttgatc tttatgcaaa
    24121 tatttttgtg atattttaca tgcttgattt gcaaagtatt gagctctttt aggtccaaat
    24181 ttatttttta aatcatcaaa atatattcct tcttttagtc tcaatcttgt cattacatat
    24241 tcgttataag cttcattttt atttatgatt tcataatcat actctaatga attattttct
    24301 aaacctatta aatatttttt cagagaagtg acattccaaa atcttactgg gtttatgtaa
    24361 ctatgagctg aaggtccgaa accaatataa tttttttgaa tccaatatgc tagattgtgt
    24421 ttacttttat aattaggtaa tgaaaaattg gaaatctcat aatgatgata accattttcg
    24481 ctagcccatt gataccattg taatgtatta ttgatgattt tgtcgtcatc tagcatgagt
    24541 ttggggttgt tttttataag tttggctaat cttgtttttt cttctatcgt caataaataa
    24601 gttgaaaaat gtggaatatc gttgtttatt aataatttga aattttcctt taaatcatct
    24661 atggtctcat ctggcagacc tattataaag tctacactaa gattattaat accttgtttt
    24721 gaaataatat taattgcatt tctaatatta ctagcagtat gttctctacc aagaaatttc
    24781 aaatgttttt ctaatatatt ttctactcct aagcttactc tattgatacc aagatttttc
    24841 cattcactca ctaaatcttt tgttatatca tcagggttgg cttctattgt tatctctata
    24901 tcatcatcta gagtgtgatt ttttaaaact acattaaaaa tttgttctag atctttacta
    24961 gtcaataaag aaggagttcc accgcctaaa taaatggttc taatattttt agcaatcaaa
    25021 aaatctttta attttatttc ttttagtaaa gcactaatga ataaatcctt tgtgttatcg
    25081 ttattatcat aaatagtaga ataaaatgaa caataaggac aacgtttttt acaaaatggt
    25141 atgtgtatat ataaatgttc tggatttgta taatttttat taacaagcaa ttcattaatt
    25201 gagtaattgt catttatact gtttgccatt aatatattta taatttataa tattgtattt
    25261 agtcgatatt tttagatttc aaaattataa attaaaaata tatcataatt caataaatta
    25321 attattatta caagtatgct aaatctaaaa aaaatcattt attttgcatt ttataaaaaa
    25381 atttaaaact atggaaaatt tttggactta tacattatgg ggtaatacaa ttaaagatta
    25441 tttaatcagt atatgtgctt ttacgatcac ggcattgatt ttatggttat ttaaaaatgt
    25501 agtattaaaa agattaaaaa aagtaaccaa gcgtactaaa tctaaaattg atgatatttt
    25561 agtgagttgt ttaactgtta ttaaatggcc attatatcta gtaatagcat tatgggtagc
    25621 attcaaattt attgttattt cggataaact aaatcaaatt tacaattatt ttgtaataat
    25681 cgttattgtt tactatgcaa caagaattat tcaagaattg attaattatt tcactacaga
    25741 tataagcaat aaaagaaaag aagaaggtag ggcaacggat gcaggtataa taaaattaat
    25801 caataatata ctcaaagtta ttctttgggt ggtagctatc ttgatagttt tagaaaattt
    25861 tggttttaac attaaaacga ttttagctgg aataggtata ggtggtatcg cgatagcatt
    25921 tgctttgcaa gcaatactag gtgatatatt agcatgcttt tctatttatt ttgatagacc
    25981 ttttgatata ggcgattata ttacatttaa tgatatctca ggtaacgtta aaaaaattgg
    26041 tattaaatca actaaaatag acagcttgtg gggcgaagag attattgtat ctaataaaga
    26101 tttaagtaat aatattgtaa aaaattataa aaggatgcaa aaacgtcgta tcgaattttt
    26161 ctttggcgtt ttatatgaga ctcctatcga aaagcttaaa atgatacccg atatcgtgag
    26221 agaagttata ggtgaaatag agatttgtca agtagatcga gttcatttct acaaaattgg
    26281 ggattatagt tttaattttg atgttgttta ttatgtttta gaccctgact acagtaaata
    26341 tatggaggta cagcaacaga ttaattttgc cctaaaagaa agatttgaaa aagaaaatat
    26401 tgtttttgca tatcctactc aaacagttta tctgaataat aataataatg aagttaccaa
    26461 atcctatgtt ataaataata aggttctata acgtttattg tgggtaaaaa cattttagat
    26521 tgttacaaaa aaataactat tttttaatta tttaggttat ttttttacca caaagggcac
    26581 caagttatta cacaaagtcc acaaagtgcc atttatagtc cttttaattt catccttctc
    26641 tgtgttcttt gtgcattctt tgagaacttt gtggttaata tttaactaca ctataattaa
    26701 taaactaata ttaacaaatt ttttataaaa ttactctttt tttaatcttt atccatacaa
    26761 aacgttatag aaccaataat aatatttaaa aaaaattaat atttggaaat tctaaggttt
    26821 tgcatccatt tcagctttta tattttttag attgttactg agagcgatgt tgccgatgta
    26881 atgaattata attatttttt tagtatataa ttatgtactc ctaattaatt gcttattttt
    26941 ttgaaataaa aaatttttac atatctttgc aatattaaaa ctaggggtgc ccaattattg
    27001 gctgagatca taccctttta atacctgatc aggataatac ctgcgtaggg aagtaaaaat
    27061 gattatctca tcctttaatt tattcccttt taatttattt agtaataaat ctcaaaataa
    27121 aagataaaaa tggaacagat cgtatctaat ggaatcatag attcctattt caacaaatta
    27181 aaacaatatc tatctgtgga tgtagcaata gtaggtggtg gaccatcagg attagtagct
    27241 tcatattatt tagcaaaaaa taattataaa gtagccgtat atgaacgtaa gcttgcacca
    27301 ggtggtggta tgtggggtgg ggccatgatg tttaatgaaa tagtagtaca aagagcggcg
    27361 cttcatattt tagatgaatt atcaataaaa tatcgagaat ttgaaaatga tttttatgta
    27421 atagattctg tgcatgccgc atcagctcta atatataatg ctactaaagc gggtgtaaaa
    27481 atttttaatt gtacttcagt agaggatgtt gtttttttag acaataaagt ttgtggtata
    27541 gtacttaatt gggctcctgt agtacgtgaa cgcttagaag tagatccact tgtaataatg
    27601 gcaaaggctg tcgtagatag tacggggcac gattgtgctg tggcacacac tctagaacga
    27661 aagaataata taacattaaa tactgagact ggtaaagtaa ttggcgagcg ttctctatct
    27721 ataaatgaag gtgaaaactc tactatcgat aatactaaag aaatatatcc tggccttttt
    27781 gttactggta tggcagcaaa tggagtaagt ggtagtttca gaatgggacc catttttggt
    27841 ggtatgatat tatcaggaaa aaaagtcgct gatttaataa ttaataaaat aaataaagat
    27901 tgataaaaaa atacatatga atatttttaa attctgttaa caatgagaaa ttttggatta
    27961 tatgtgataa ttacttctcc gcaactacca tataaaacta ttgcagagaa atgtgtatat
    28021 cataggatta ggatgttgca attgagagaa aaaaacttat ccgataagga attaattaga
    28081 gttggcaagc aaatacgcga aataactaga ggtacttata ctaattttgt tattaatgat
    28141 cgtccagata ttgctgtgat atgtgaagca gattaccttc atctaggaca agatgatata
    28201 cccatagaag aagctcgcaa aattgttgga aatatgaaga taggtttatc aactcattcc
    28261 attaagcagg ctaaagatgc attgagcaaa aaaccagatt atataggatt tgggccgatt
    28321 tatccaacaa atactaaagc tatacctgat aaaccagtag gtgtggatca actaaaggaa
    28381 gtgttacaat tttcgaatgt gccagtggta gctataggtg gcatagacaa tagcaatata
    28441 ataaacgtaa taaatactgg tgcccaaaat gtcgcattag ttaaatattt aatgcaaact
    28501 aatgaattgg aaaaaagaat tatagaaatt aataaattaa taaataataa atcatgagta
    28561 cacaattaca aaaagcccga cagggaataa taactcctga gatgcaagag gtggcaaata
    28621 tggaaaacgt gaatgccgaa tggctgagaa atgaaatagc tgagggcaga atagttattc
    28681 ctaaaaatat aaatcataaa tttattgctc agggaatagg caatggatta tccacaaaaa
    28741 taaatgctaa tattggcaca tctagtaagc attgcgatct aaaggaagag ttagaaaaaa
    28801 tgagaatagc tgtggaatat ggtgctaatg ccattatgga tctctctacc ggtggcgatt
    28861 taaacaaaat tttgacaaaa attattaatg aatcatcagt tatggtaggt accgtaccta
    28921 tatatacagt tattacaaat ttgttatcag cgaatagaga gatttctcaa ttagatccag
    28981 aagagttatt tgctgagatc gaaaatcaag cacaaatggg agtagatttt atgactcttc
    29041 attgtggagt tactaagtct tccctagcct ttatgaataa tgacaaaaga gtcttgggta
    29101 tagtcagtag gggtggttca ttactcaaaa ggtggatgat agaaaataat agagaaaatc
    29161 ctttgtatga tcaatttgac cgagtattgg aaatatgtgc aaaatatgat gttactatta
    29221 gtttaggtga tggtttgcgc cctggatgtg gtgaagatgc tactgataga ggtcagatag
    29281 ctgaactatt aattttggga gaattagtcg agcgtgctag agaaaaaggt gtacaagtaa
    29341 tggtagaagg tcccggacat atgcgattag atgaaataga agctaatgta aagctgatga
    29401 aaaaagtttg ttgcgacgct ccattttatg tattagggcc tttggttaca gatattgctc
    29461 ctggatatga ccacattgta ggtgctatcg gtggtacagt agctgctatt catggtgcag
    29521 attttttatg ttatatcacc cctgcagagc atctatgcct tccagatgta gacgacgtta
    29581 gagaaggcgt tatcgctgct aaaatcgctg ctcatagtgc agatattgtt aaaaatatta
    29641 ataatagtag agaacaagat tttatcattt caaaagctcg tcgtgagctc gattgggaag
    29701 ctatcttcga aaactcaata gatccagagc ttgcaaaaaa aagaaaactt gattctgaaa
    29761 gtgctgagga ggatcattgc accatgtgtg gattgatgtg tgctatcaaa aatgacaaag
    29821 aatcatcttt ttagaatttt gcaatagtga tgggttaaag aatataggtg tagagcagta
    29881 tacatgcagg aaagggaaat gttttttttt caaataaaat aatatgattt attttttgac
    29941 aatagcagct tctgataata gtggtggagc aggtatacag caggatataa aaatcgctga
    30001 tcgattaaat tgctggcctt tgtctgctat tactggtatt acggtgcaaa attttaataa
    30061 aatatttact atagaacctg tatcgccaga tctattgaaa tatcaaatag agaattgtat
    30121 aaattatttt cctattaata gtataaaaat aggtgctatt tgtagtaagg agaatattat
    30181 gatcattgca gagtgtttaa aaaaatttcc acatgaaaat attgtaatcg atccagtgat
    30241 attttctacc agtggtgccc agtttttaga taatgaatct ttaacaatat taaaacaaga
    30301 acttttccct cttgctcatt tggtgacgcc aaataggcat gagtttgaat tattggttgg
    30361 acaaagcatt aattctcttg atgaagccgt aaaaattgca aaaaataaat gcaatgaatg
    30421 gaatactgca attttattaa agggtggtca tttcgagggt gatattatca gagaggctct
    30481 aataactcat acaagtattt attattttga aagagtcaga gatatatttc attatagtca
    30541 cggcactggg tgcactctat catctgctat agcatgttat cttggcatgg ggagaaccat
    30601 ttatgattct tatttattag cttctgaatt actagtcgat ttttataaaa atattcaaaa
    30661 agattttaat cctttaatat cataattagc ctttatatgt ggctcgtaat atggtaatga
    30721 tttttataat taattgaaaa ttaattataa aaatgtatat tttgtgaatt tcagttggtt
    30781 ttattggaat tgtggacctt taaaatccca ttccaattaa gggaaactgt gttattattt
    30841 tctagtttga ttatagaacc cggtgaaaaa aaataattta ttttagataa acttttaccg
    30901 ggaatttttt tgtattcatt ttcagattcc gattctgtat ttttctcaat atcaaaataa
    30961 cctctacccg taattggttc tatatcacct ctaaattgca aactcttgtc atactgaaga
    31021 tgagatatta tgggttgatc ctttttaatt tctatcaaaa agtcatctcc atttaggttt
    31081 ttttcttcta ttgggaatac tttattattt aatgtagatt ctaattctac taatccaaac
    31141 ccatagcctt gttctcctcc aagagttagt tcttcaatca ggttaaaatt atttacaaaa
    31201 atcccatcat ttttaatttg aacttcgtaa tccccaagct tagaattttc ttttacccag
    31261 atacaaccaa taatttttgt agtttttacc tcaccgtttt cgtctatata cttatctttt
    31321 ataaattcga cttcgtgaag actctcgtct tttgcagtcc ctgaattttt atctattgcg
    31381 gtcaaaactc tactcccgat aaatttgtgt tcaaactccg gtaataaaag attgccatat
    31441 tttagctcat catcggcaaa actgggggta tatatagttt tgtcatcata aagatagaag
    31501 tatgaaaatc taaaattatt ctttatctta tcacctactt ctctatagtc gcttccatga
    31561 ggatctttat acaaatgttc agttgtacgc tttgtgatgg caccccagaa atttttaccc
    31621 ggaacgtagt accttgtagg tgatattaca gagcccttaa aaggaagata tccgatatga
    31681 aggggacttt ttaatgtgaa aataacatca attctttccc acattttctt cctcttttat
    31741 ttttgcatga tataaagcat aggtgagagt gcgttcaagg atttttttaa gaaatagcag
    31801 tttatcaata tccttagcaa cttcttgaag cttttcacaa aatcctttaa agtcatcagg
    31861 aaaactttta gaatctgaaa gaagtttgct tgttatttcc ttctcattta gtaaactgtt
    31921 tagctttgtt cttatttcat tatctttatt ttcgagccag agaaacatgg catatacacc
    31981 atcttcctgt aaaataccaa gagcttttct gagagtattc tcttttttat ctttttcatt
    32041 ttctactaag ttctgaccaa tttttgcaca tatataatca agatttatca tcttcgttac
    32101 cctccatttt attatttgac tgcttattgt tttttaaggt gaaaaaatca acccttccaa
    32161 aaccacgcgt tcccattcca cccagtccaa gaagttttag atatggaaac gtcgcttcta
    32221 tcaaattaac gatgctctct acattatctt cttttctggt atctgtaacc atttcaaatc
    32281 ccagaatcgt tcctcttggt attgcttcat aagtgaataa tgcaccttct gcggcagttc
    32341 ctgtttctgg atcgatcctt acagatgtgc gcacttctaa attgtcatta actatatgag
    32401 agaatagttt atcagagact atcacaatcc tttttaagaa tttatcaagt tccggcggca
    32461 atgtaatttc ttttccttcg ttaatattct gagtatccaa tataagccag ccaaaattta
    32521 gtggttcaga actattgatt ccctttattg cgtaggcctc atctttaatt tcttcaggaa
    32581 ttgttattgt ttctcccttt tcatctttta tttcacagag ccaatattcg agtaattctt
    32641 ttgttgttat ccagactgtt ccttgaatag aagatacggg aaaaaagatg atttgcccat
    32701 cgtagaacct tagcactccc tgtctgtttt cgtcaccaaa atattttttg acttcttctt
    32761 cagcatattt tctcttctct ttttcatcag ttttactttg gcaggcattt ttcattgtat
    32821 agacagtagc gaactctctt attgcacccc caagcgaagt tcccggtatc tttggcacat
    32881 ctgtggcagg gtctctgaca atggtattgt ctaccctacc gagtctatat ccaccggcac
    32941 cgatatgcac cggatcaagg gttattccga aatatttttt aggttcattc attttgtttg
    33001 ccctcctttt attgtatgat tgaataaaat tattgtatcg agtaaaagtc cattatatgc
    33061 agagtttaaa ataagatctt gcatttctat tcttaaatta ttccagtttt cactgaaagc
    33121 atctttaatc gtagcctctg caaattttgt aaaaacctct tcctggtttg catcactgac
    33181 attacgccat tctcgcagtt tgtttgtaaa catttcttct ataaaattaa tctgcgagcc
    33241 gggaatattg tttgttaata tttcccataa gtccatcatt tctgcaaatt gataaaaata
    33301 ataaggtcgt ttggaataga gtttataatc tttaccggct ctctttttgt ccttgtagaa
    33361 gatattgtat ctatcgtcgg tacccagaag caaatcaaag tcgaagtatc cgggatataa
    33421 tgcaaatttt ccccctttag ataggcgggc cagatcatca agattgtaag agtctttttt
    33481 gtttactaca ggataaaaac tgtaatattc atcatttcta agtccattta tatcccacca
    33541 tacatccatc gactcgggat ctttgaattc tttattttta aacattctac ctccggcttc
    33601 cagaagcata tacaggggga attttctgtt cgcaacaagc aaacccatgt taaggggtaa
    33661 tttcccaatt actttttcaa acctttgatt atataatttt acgatcgact ccagaatttt
    33721 tatagaatct gatgccggta caagtaatct tagcgataac ggtgattttg tgatttcaat
    33781 aaagggataa tagtattctt ccgatatttg attcttaatt tttacatttt gatcattctg
    33841 cagtagattg gtgtcagggg catcttctgt tgaaatccag tttattcctt tacgaaaagc
    33901 ctccttaact gcttcaacgc ctgatttttc tccacatcta tacttttcaa gagattcaat
    33961 ggtatagaat tcaccatttg tggaatgaaa tacgagaaga ttttggggat tcaaattatt
    34021 tatcttaatt acgaaaggag ttttagattc taagtcagtg taatccaaag aaaattttat
    34081 tcttttccat ttattttgat aaatctcact atttatctta ttaataatta aatcaaagaa
    34141 ttcttttgtc tctttccaga tacggtataa acgagctgga gatgggtttt gagtgaaaat
    34201 taatttttgc aaattacatg aattgaattg ggcaggcttt atgcgttcat ttatgttgtt
    34261 aataaatttt tctacataat tctcatcttc tattgtttgg tttgatttta tgtcttcgta
    34321 aaacgtattt attatattac ttttaaaacc aggatctttt gtaatatttt catcggtaat
    34381 aatttttaaa agattttttg ataagttaat aaaagtatcg tcaattttta taccttgttt
    34441 ttccaacttt tgtgtatttt gtttattatt gaagaactta atagcatcat tactattttt
    34501 ccagtcctca aaagtttgcg aaaaaattgt accaattatt gtcccatcga gccatttttc
    34561 aaggtcaaag ttcaaagtaa gcaatgcaac tcgattattg gtgtctgcga tctcgtcgag
    34621 ccagatggta tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtaagcaat
    34741 gcaactcgat tattggtgtc tgcgatctcg tcgagccaga tggtattttt tctgttgcta
    34801 agccattcat ttaatctgcc tgtttttctt tcttcacaaa cattgcatct ttcattttct
    34861 atagattttg ctctaaattt acatacagga caaatatcta ctttattttt atcttcatct
    34921 tcatcttttg gagttggcat atcaagacta actaataatt tttcttcttt tctatcaata
    34981 aaaagtcttg ggctcatctt tgggatattt ctattttcag cagcaaattt caattctcca
    35041 gcaaggatag tgaggcttct ggatggcgtg ctcaaggtga aaaagggcca tatttcatta
    35101 ttgctaatat tataaataat cttcaaagct tctttcgcac attctactgc aagtacttct
    35161 gaattttcat tcaatgcagg gaatgtaaaa tagattccat taatatcttc ataaatgaca
    35221 tttccgattg gtatttcaac ttcaaatttt tctgtcaggt tatttttgat atcttcaata
    35281 atttcgtttc tattcagaat atcggcaatt ttcctacctt tatttataaa accaatacca
    35341 tcccagcaaa atccaagaat tcgccattgc aatttactat tatcgggatt ttctccaaac
    35401 accattgcac acaagacaga tttaaaaagc gatgcagtgg aatgtgaatg gtcccagagg
    35461 gtaacatcgt ttgctggaat ccttgtttct ccaagggcat gagaaaaagt ggttttgagg
    35521 ttattgatta aagactttct aaaacagtct atactcatat tctcagaaat ataattctga
    35581 aataaacctg ttaaattact ttgcagatca tcaagtcttt tttgtaaaca tttaagttct
    35641 ataatctcct tatcaaaacc aaacggcgaa ctgattatgg cattatcttt tgattgtttt
    35701 cttctgacaa ttcctttatc gtcagcagaa tcgagacgat cacaagattt taatatattg
    35761 atgataatat tatcattcct attatgatgt tctttaatta aagaatatac actggtattt
    35821 tcattattga ttataaattc aagtttactg ctcttgacta aatcaacaaa tttgttctct
    35881 aaaaattcat caatatttgt atgatcaaaa ttagttggtg tattctctat tgagtttttt
    35941 cctataaaat cgggatggca tttcccaata tcgtggagca atgcaccaat ctcacctgtt
    36001 agaatgtgca ctctgtcgtt tcttattata tcaagcattt cttctcctgt tctcattttt
    36061 atgaaccatc cttaatattt tcgttatgtt cggtgtataa gattgagaat aattccctct
    36121 tgtcacacgg tttaacctca acttcgctgt cttttaatct ttcaatcacc ccgaatccag
    36181 aggttttttt agctgaaaat ccgtatgtat aaaacataag ctccaatgct tttgctaaaa
    36241 aaggcaagtc ttttttaatt tcttcttctt tgaactcgtc gcctctagga taaggaaaat
    36301 ataagagatg aaaatcacct tttttgccgg gtttcataac ttctaaactt attggacctc
    36361 tctttgtagg ggttctggta tctcttttca aaggcgttat tacatctttt tctgcatctt
    36421 cattgaaaaa tgtagggaaa aagtaaagtc tccctttcaa tggattatct ttttcttccg
    36481 gttctgaacc aaaaagtcgt ttgataatta tctttttttc ttgctcattt tgccattcaa
    36541 ctttatttgc agcaaatctc aaatgtcctt tccatgttgt aggcttcatc ataggaagtc
    36601 ctgtgaactt atctctaacg ataggatttt cgaaaatttt attatctatt atattaaatt
    36661 ctccttcatc tttgcttgtg tatggctttt tgagggtgaa ggagattttt aaaagagtgg
    36721 agtcctcagg aagatttttg agatcagaat ttgtaggata aaaattgtaa tcttgatgat
    36781 catttaattt acccataatc tcttttttct ttttgccaac ttctgatatc tgatcatcta
    36841 tgtttttaac taattttgtg aggctaagat aataatcgtg tatcatttta attcctccaa
    36901 taattttaat ccttcaatga aaagaattgg tttcttattt aaaaattttt ttatacaatt
    36961 aaattcatca tctttttcag cgtaaacaaa aagacgattg ggctttttat ttatagtttt
    37021 gtaaaaatat ttttttcctt tattttttgc accttttaaa aatgatagat tgttctttat
    37081 ctctataaat gttttattat tttggttttt aataaagata aaccttgata gatttggatt
    37141 gtcaacacct tttactttct tcctaagata attatttaat tcttcctttt ttaagtcaaa
    37201 tttgtctaaa ccattttgtt ctatctttat aattccatac tcagcttctg caatttttcc
    37261 tcctattgct ccataatccg aaattatctt tatagtttta tttaacaaag cccactcaat
    37321 atttttcatt tctctcattt ctatgaatct taaatcgata gtttcgttat cattcttttt
    37381 ttctacttcc aacctgaact tccttgccca acctgtgcat ccaaataact cacatgcatc
    37441 acaatgatta tccccattgc aatttgtgtt tgttgggtcg catgctgtgc cgcccaatcc
    37501 cctgattagt gcttcatacc accagcgtaa gctgccaatt atacctgttt ctcttaatgt
    37561 agtatttttt ctatctgcat ctcctgtcca tattggagtt aatgctttga ttttgacggt
    37621 atatttattc atttcatatc ctccgaatat tttcttttca taattatctc taatctatat
    37681 tattatttat gtagtttaaa ttgcaaaaat aataatattt ttctataaac caaattattt
    37741 ttttgttggg acgattaatt gggtagattt gtgaattacc cataaaaggt ttttttatta
    37801 tttattatcg catccattat tcagaatatt tcagggctaa agcccattcc tctgtttttg
    37861 gttgttccac gacctaaagg tcgtggttaa tatctatccc tttgctattc gacctaaagg
    37921 tcgtggttaa tagatggtat gattatttgt tcatacaagg tgtttttcat tgggggttga
    37981 tattatattc atcatttttc caattctttg gattgttcgc aatataatga cgaatgcgcg
    38041 ataaatcatc ggtatctcga atgatatgtt cgtaataatt gcgttgccag acaggaatgc
    38101 caggggtttg acgaa
//
LOCUS       DAOPRN010000013        36350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_901_length_36350_cov_104.514975, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000013 DAOPRN010000000
VERSION     DAOPRN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 36350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36350
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_901_length_36350_cov_104.514975"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PKI65_02655"
     CDS             <1..1018
                     /locus_tag="PKI65_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB77376.1"
                     /translation="YPTQTTTYYGTISDTLHGNSATDSITIVVIPQPSIDLGPDTSVC
                     EGEIITLSPGAGYDEYLWSTGYTNAELMLDMSGVYSVTVANTYDDVSCFDSDTIKVVF
                     VPNVNIDLGQDTCVTEPIILDATQPYLDISYLWSTGNINPAIEISNTGTYWVSAVNSI
                     YNICGDSDTINVKVIPEPILSLPNQITICSHESFTLSASQSNNNLYEFIWSTGSTEPI
                     YVLDKPQAGIYDISVKVIGCDTVYGSTNVIVENCEISIPNVITPNDDGINDVFYITNI
                     EFYPNSEIKIYNRWGKKIYENENYQGNWDGKGLDDGTYYYIFRLNYGYGKIDEYHGTI
                     TIIK"
     gene            complement(1302..4229)
                     /locus_tag="PKI65_02660"
     CDS             complement(1302..4229)
                     /locus_tag="PKI65_02660"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOB77377.1"
                     /translation="MERRIIFTIVKDKYFDWMVVPYEVSILKNGQFSAASKPITLKNV
                     TIEIDEATRKALELCSMMTYLHIAKVFHFNPLTFEEDLATINDKTFEIIRNYLRDKTD
                     KLLEILEKNNIPLYYKGERDNVIINEEPIHFSSKSLDIIFIFDRSDDGIKYSLKANVG
                     DNDIKLSKSDTFILNYEPCWVIHKSILYTTQPGIDGKKIEPFLDKQQIFIPKPHEKTY
                     IEKFIKPLLEKQLTVKLNGDFIINELQEIPKSELRLELGMDMKPLLSLFFHYRNEEIP
                     YGDKKEVFIFYEVQDAFINISKVKRNKEFENEIVDYLNFIGLKHKFHNNFILIDLNEE
                     NNEINENETLYDYINFINLNSELFANKNIELDTKTFKEKYFLGKVELKQHIKKSGIDW
                     FDLQIEVCFDDYCIPFYVLRNNILENRRDFVLPDGRVALLPREWFAQYKELAILSQKG
                     KNNVKVTKTQLKLFKEINTPQSQIDADVKQLKLDQLPDVKIPPSVRKTLRPYQQKGVN
                     WFEFLDQHDFGGILSDDMGLGKTIQVLAFLAYKKDEIQKLIPQSTQKFEKNTLFKEKK
                     LSVLIVMPLSLIYNWINEIKTTAPILSYFVYYGTNRKINQNIISKYDLILTTYGTVRK
                     DYEILSQFNFDYIFLDESQAIKNPSSKIFRVITKLNANRKFVLTGTPIENTIVDLWSQ
                     MSFVNPGLLGDLNSFRKKFMIPIEKDSHKATEKSLQQIISPFILRRTKDMVAKELPPL
                     TERTYYCSMTEDQYSYYEEKKSEIRNYLMDLKQKVGFDKAYLSILSGITRLRLIANHP
                     AIIDKNYNSDSGKFNDVIENIQKIIVSDNKLVMFSQFVKHLNLFKDYLEANDIPFLML
                     TGSTPMEERMKIVNEFQNNKDIKLILMSLKAGGVGINLTAADFVFLLDPWWNPAVEYQ
                     AINRTHRIGQNKNVISYKFIVKDSIEEKILLLQNKKLKFAQLIDTTKRIPITEEDFGT
                     LFD"
     gene            4610..5014
                     /locus_tag="PKI65_02665"
     CDS             4610..5014
                     /locus_tag="PKI65_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77378.1"
                     /translation="MHQKSKYFAFLIIFLILLSSCRISQKKNSPEYVSYQFLSHFQQM
                     EFDEAAKYGTENTKMLIMFFKNIIGMMPPEKRDSIQIPEANVEIKKCYVYENSAKCTY
                     IANEKTDTLDLLKIEGKWLVDLKKESKKAEKL"
     gene            complement(5048..6049)
                     /locus_tag="PKI65_02670"
     CDS             complement(5048..6049)
                     /locus_tag="PKI65_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOB77379.1"
                     /translation="MIKRISLFILVIVFTVSCVPSRQYEDEKLKREQAEANLQKLQQS
                     CASLENKNNELEEKIIDLEKQIKFLKQDTLNTGIALRKLKNEYDKLLQLYDILDQKNK
                     ELLQTNIDETKLISSELSLTKEQLELKESQLRKLEQELNLKKQTLDELEQELKEKEKR
                     ILELESMLVRRDSTLNDLKKKISSALFDYEDKGLNVIMKDGKIYVSLEESLMFPSGSW
                     AVDKRGEEALSRLAKVLEANPDISIIVEGHTDDVPYRGRGEVKDNWDLSVMRATAVVK
                     ILLQKGNINPSRITAAGRGEYAPIDPAKTAEARQKNRRTEIILTPKIDELIEILKTD"
     gene            6193..7089
                     /locus_tag="PKI65_02675"
     CDS             6193..7089
                     /locus_tag="PKI65_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOB77380.1"
                     /translation="MTYVTKEKLFSREWFKAYSYILIGTLTMAVGFVMFISPYRFAPG
                     GVYGISIILHHLFNFPIGITGLCIDIPLILIGMKILGARFGVKTIVGSLSLSGWISLI
                     EFTWGYDPLLKNDPLVSAIFGGILLGIGLGLIFKSKATSGGTDIIAMILNKYTRMPIG
                     QLLIIIDSIIVLCGLVAFEDWKIPFYSWIIIYITGKVVDTIIAGASYEKACLIISEKI
                     NEIKNKILFDLDRSGSIISIKGLYTGTNKQMILTVVNRREVEMLKSYIKQIDPDAFMT
                     VINASEVIGQGFKSIDEAIIND"
     gene            7324..8979
                     /locus_tag="PKI65_02680"
     CDS             7324..8979
                     /locus_tag="PKI65_02680"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HOB77381.1"
                     /translation="MQKNLFFILFLLITLINIVHAQEGKQSSQIYGDFQLDAMYYLTD
                     STISAYQPKEKLGNNAYLSLFYEYGNISAGLRFESFLPPLIGYDPQSKGIGIAHRYIA
                     YETKNRDLKIKAGHIYEQFGLGLSLRTYEDRSLGIDNAIDGIEVIYSYQPYFTIKGLI
                     GTQKFFWDKTGNYIRAIDGEIYLNKLNQKWAEGRWNFTLGSSFVSKYEEDKDPIYKLP
                     ENIGIWTIRYQLSSHFLLWNTELALKSQDPSKTNNYIYREGIGAHTDISFFGNNWGIY
                     LAAHRMDNMDFRMLRNESATRSIINYIPALTPPQNYILIKSYPYASQSNGEWAFSGQG
                     NFTFPKNTALGGKYGTQLQINYSRIHNIDHKQINDTTPFMSPGTDGYSSEFFKIDKEV
                     FFESIDFYIDKKITKDLKFNILYNYTVYNSYIIEGHDHGTFYIHNGVIDLNYKLPKKN
                     SLKIQLAHEYKEQYNKNWLGALIELAISNKWFISVQDEFNYGNPDKDHRKHYYLANIT
                     YLKSATRISLSYGKMREGIMCVGGVCRWTPAYYGFGINISTTF"
     gene            8992..9831
                     /locus_tag="PKI65_02685"
     CDS             8992..9831
                     /locus_tag="PKI65_02685"
                     /inference="COORDINATES: protein motif:HMM:NF033782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28 family outer membrane lipoprotein"
                     /protein_id="HOB77382.1"
                     /translation="MKKIFYIISIIILYIFVSCDKVEPPYIENPVIIDTNNNEGTKRV
                     LIEEFTGHLCPNCPEAAHITKVLENNYPGKITVIAIHAGNFAIPISPNYMTDFRTSAG
                     NILNNSFGVYTYPSGMVDRISVLGSQLISPNDWGNIVNERLARQLQIDLKITSQYNEN
                     NRSFQCRVEAKTLDVITNPLMLVALFTEDSIIDYQKNNNPLYGPTPDIPNYTHNHVLR
                     DCITNVWGDTLCDNCIVNNVYYKNYSFEIPNNWNADFVNLVVYCYDSATQEVLQVIEK
                     RIK"
     gene            9985..11058
                     /locus_tag="PKI65_02690"
     CDS             9985..11058
                     /locus_tag="PKI65_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOB77383.1"
                     /translation="MSAFIITIIFTLIISFICSIMEAAILSVTPSYLAYLLAKKPNQG
                     KKLEKLKQSIDRTITGILTMNTIANTLGSALIGMFAAKIWGSSAAGIVGFIMTFLILY
                     FSEIIPKTIGTYYWRKILPWMIPVLLFLRKLTYPFYLLAQGIMKLIIPKNEPSHTIEE
                     IRALTLLGYQKGILDNIQTQVIDNTLKLKELPVHNIMTPRTVTVVLDENLTVGELINL
                     DDFFKYSRIPVYSENIDNITGYVFKNDVLNALIHKNEYTKLAEIKRKIIIIYEFFSIK
                     KALDELTKNNEQIAIVIDEYGSFVGIITIEDIVETLLGIEIIDEADQYKDLQEYAKEL
                     WQMKMNKLGIFKSEEENQNNPDL"
     gene            11106..12284
                     /locus_tag="PKI65_02695"
     CDS             11106..12284
                     /locus_tag="PKI65_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HOB77384.1"
                     /translation="MQQIILIDRYREHLLPIVFTKPIAEIRVGIMTIKEKWEKYTNAK
                     VDILSADYLKNKFPTPETDQDTIWIDARILPNSNLARTVLNIDKKQLLIDTNGDFVAL
                     RNKEFLNMLHNNELDSGINHVIYQDEIELIKKTWDIFTHNGNEIKKDFLLLTNGRDSA
                     KIPDYVYATKVENIFLEPGVKLSPCSLDAEDGPIYLGKNSEILDFATIKGPFAILEHS
                     QIKIGAKIYGNSTIGPQCKVGGEINNVVFQSYSNKAHDGFIGNAYIGEWCNIGADSNN
                     SNLKNTYEKVKMWNYAINAYENTGLQFAGLVMGDHTKLAINTMLNTGTVIGCSCNIFG
                     AGFPRTFIPSFRWGGAGGFRVYDFDKAISTASNMMNRRDKKLSETDIEILKYVFEKET
                     "
     gene            12420..14846
                     /gene="lon"
                     /locus_tag="PKI65_02700"
     CDS             12420..14846
                     /gene="lon"
                     /locus_tag="PKI65_02700"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOB77385.1"
                     /translation="MTELEELMNNISFFMSDGGEDFFPVISADDEKHISSERVPKELP
                     IITLRNAVLYPGSIMPITIGRDKSIKLINDVSKTTNIVGVLAQKDANVEDPTFDDLYQ
                     IGTVGKIIKILQMPDHTKMVILQGRNRFKVVEPITTEPYHIAQVQKVKPISRPIDNAE
                     FLATIDLIKNCAKKLIKRSPNIPDEASLALDNIQSPVYLLYFVSTSLNLPIEKKQQIL
                     ESDDALEIANIVLEKLASEIQILEIKEKIEERIKSDIDKQQKDYFLNVQLKAIQDELG
                     VSTNEQELKELYEKSKQKKWSDEVAELFEREFKKLQRMNPAAAEYSVQLNYLELLVDL
                     PWNEYTQDNYDLERARKILDEDHYGLEKVKDRIIEFLAVTKLNGNMRSPILCLVGPPG
                     VGKTSLGKSIARALERKYIRISLGGLHDEAEIRGHRKTYVGAMPGRIIQSLRKVRAAN
                     PVFMLDEIDKVGSLSMQGDPQAALLEVLDPEQNTHFYDNYLETEFDLSKVMFIATANT
                     TATINPALLDRMEVIELHGYVLEEKLEIAKRFLVPKQLEENGMADSKLKFSDNAIREI
                     IENYTRESGVRALEKRIAKVIRNKAVDFASGKLKKTTLTVNDIQNILGVPIFKREKAL
                     NQPTIGVVLGLAWTPVGGEILFVEASTHKGDGKLSMTGNLGDVMRESATLAFEFIKAN
                     AISLELDENFYQKVNIHIHVPEGAIPKDGPSAGVTMLTAMASVLLNRAVRNDIAMTGE
                     ITLRGKVLPVGGIKEKILAASRAGIKNIILPIDNKADFYDIEKHYTKDLNPIFVENMI
                     DVLKEVLVKD"
     gene            complement(15118..17631)
                     /locus_tag="PKI65_02705"
     CDS             complement(15118..17631)
                     /locus_tag="PKI65_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HOB77386.1"
                     /translation="MKKLFALLTAFILLISCVYSQNTSQNVTSKEMPEFTISGKVIDS
                     LSNNPLEFVVIALYQTKDSILVNGTTTDINGNFVLKHKGIGNFYIQVSFVGYANKIIP
                     NIILNPRSGNFNINIGTIKLRPSAYLLQSIEVVAEKPMIELRADKRIINVDQVLTSKG
                     GTALDVLENVPGVEVDEDGNVSLRGSQNVTILVDNRPIALLGDNKTILEQINAEEIEN
                     IEILTNPSAKYSPEGTSGILNLQLKKQKSFGLNSMIRVSGGMMNENIEDFSKFKPFLS
                     PFSAMFAISYDAKKFTVNLQYSPRYNKHYEKGSTYTENYNLNEMHYLNQNNYGNHGGW
                     NHNLQGTIEWRPNNNNTLGFYAAYRYRLNSGESTTWAEYLDTLKNPTQIYNIIRTSND
                     TSQNMSFALYYTHKFPKKDHKLDVNASLYPSYSPRMNIIKRNYFESDYTTPSETMQPF
                     WNKTFSISDNLQGYLKIDYSYPFNDSTKIEGGFESNLRIYDMDTRYYKYLFNENIYLL
                     DSMQSNHFLYDEYVNALYAVFNTKIKKWGFSLGLRGEMTNTNARQADSTDNKNTYYSL
                     YPTGAISYNINKKQQIQFSYSRRVYRPWFGYLNPYIDYSSYPYISSGNPFLKPQYINS
                     FEISFMQYFKKGLIAPSIFYRKTTDMISSYIKQLNDTVYLTTFENFKSSNSYGLEITF
                     QYRIADYWNLMLNTVFHYNVIDGSNVDASVKSESYGGFARLNNQFKLPKNWNIQWMIM
                     YRLPTETGQGKRGGFLNSHLSVQKSWDRLELSMTVRNLFNTAHFKMEYYQPTYYSLIK
                     NYHGGLGIRINIKYKLFGNYKEKHLNNIDEMENNGTNMF"
     gene            17769..20306
                     /locus_tag="PKI65_02710"
     CDS             17769..20306
                     /locus_tag="PKI65_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOB77387.1"
                     /translation="MLKNYIKTSNFTKKIRNNFIESLYLLSILLFTLIFNTNLFSQDI
                     AFSSNYFPNNPGLKEAKKNIKNGNSLFIKGEGYYTEALEYYLKANQFNSDNAELNYKI
                     GICYLYSADIEKALEHIEKAYRLNKNVNDDITYYLAQAYHLNLDFDKAILYYQTYLNV
                     LKPRDRDKHLSKINKKIEECNNGKELIKDTVKVLIDNLGGTVNSIYPDYAPVISADEA
                     MLIFTSRRPGNIGGMRDPINGKYFEDIYIAYMEEWGKWLLTQPSAPLNDKYHNAAIGL
                     SPDGQTLYVYKGVNGGDIYVSYLKGKNWTNPEPLPAPINSKYHESSASLGPDGRTLYF
                     VSDRPGGIGGRDIWMARMDNMGNWTNPINLSSINTPYDEEGVFIHPDGRTLYFSSKGH
                     NSMGGYDIFRSVYQDGKWSKPVNLGYPINTPDDDLYFVLSASGIHAYISSSRKGTLGD
                     QDIFMITYLIAKPVMPSSEANLLAWRGKPSIYATGSQSLIAENLPSMILWKGKVIDEE
                     NNPLEAQIILTDNVTGEQLAVFTSNSETGNFLVSLPSGKNYGIAVKKEGYLFHSENFN
                     IPENAPYEELNKDIILKKVAAGAEIVLNNIFFDFNKATLRPESKTELQNVIEFMQQNP
                     SVIIEISGHTDNIGSYEYNQSLSERRAKAVVDYLVEHGIPLSRFVYKGYSFSEPIATN
                     DTEEGRQLNRRVEFKILAADEVYKSDTYIQTAKSETKTIKAEVQPIETTKTTKLIVEE
                     EVKKVEPVKEEFKKEVPAKEIIPTKPTPQKLFHIVGASVTNINDAEKIKNEYIKKGYI
                     DATILPRSDGKGYRIAYKSFTTKEEAIKELEILKKETKQKDLWILQQ"
     gene            20343..21383
                     /locus_tag="PKI65_02715"
     CDS             20343..21383
                     /locus_tag="PKI65_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB77388.1"
                     /translation="MKRELILSLILTFIMSIVNAQAVKVGESQIAEQMCNQGVMLLEA
                     GDLQSAIAKFNDAIFLNPNYAIAYYHRAMAKSKIGLYDDALNDLNKALNIEVNGMYYY
                     SRGMVKQEKGDFQGALQDYNAAINTGQNLAEAYYYRASLYFDQGDYNSAIDDLKQAIS
                     KKDNFAYAYNDLGSCYMMLEDYDNAIFYYNQAIKADPKFSIAYNNLGITYKNMKKYDE
                     AIMEFTKAIDLDPDYVIALNNRGVAKLEKGDYKGAADDFTLVIQKDPDYYFAYNNRGI
                     AKYKIEDYQSALEDFNKAIELNENYAVAYLNRANTKEMLRDMDGACNDWQKAGEFGIK
                     AGDMYYKQMCNK"
     gene            21495..23306
                     /locus_tag="PKI65_02720"
     CDS             21495..23306
                     /locus_tag="PKI65_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOB77389.1"
                     /translation="MKIKIFAIALLLASIVYGQVDTPFDKKHITDSKQLKHALKNIKE
                     GNKLFGLGKGRYIDALEHFLEANKVNANNADLNYKIGICYLFTTDKELGLEHLNKAKR
                     LNPQVTYELSYYLGRAYHLNHQFDTAIVYYNEFKLNLYKRELKIRAFEIDKLINECEN
                     GKELVKKSEKLLIDNLGPNINTIYPEYAPIIIANEEVMFFTSRRPTTTGGKRDPIDKI
                     YYEDIYMATKDNDNNWQQMPVTELNTNKHDAVVGYFKGKELSYLYTYKNKHGGDIYIS
                     LIKNNKIDIPKPLPFPINTKYHESSASLSPDSSTLYFISDRPGGIGGKDIWMSHKEYN
                     QMWSEPINLSDINSPYDEQNVFIHPNGKILFFCSNGHNTIGGFDIFYSIYQDGKWGKP
                     INMGYPINTAADETNYTLSSCGSYAYISSNRQGTIGDIDIFKISYTIKKHVLPSSENE
                     LVAWQALFKKLSEKETKNLLTKTENMKDLPSLTNDNLYNEILWSNNIFFEFDSIALSS
                     ESRTVLDNLIKLMNDNPDIKIEISGHTDNIGSAAYNQKLSEARAKAVVDYLVEHGIDR
                     SRLSYIGYGFEKPIATNDTEEGRQLNRRVEFKIIQVN"
     gene            23325..24374
                     /locus_tag="PKI65_02725"
     CDS             23325..24374
                     /locus_tag="PKI65_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB77390.1"
                     /translation="MKRIIILCIIGMVTMSFLEAQDTIKIISDNLQIAEQRYNEGVKL
                     LESGNIQGAINKFTEAINLKPDLSKAYYNRGVAKSQAGLYDEAIADLDKALSYETSGF
                     YYYTRGKAKHEKGDLEGALEDYNAAINFDKNLAEAYYYRASLYFDQGDYNSAIDDLKR
                     AISKKDGYAYAYNDLGSCYLMLEDYDNAIYYYKQAINADPRLTLAYNNLGTTYKRMKK
                     YDEAIIEYTKAIDIDPDYVIALNNRGLAKLEKGDYKGAINDFTEALKKDPNYYFAYNN
                     RGIANFQLEDYQNALEDFNKAIELNENYAVAYLNRANTKEMLRDMDGACNDWQKAGEL
                     GIKTGDMYYKQLCNK"
     gene            24388..26430
                     /locus_tag="PKI65_02730"
     CDS             24388..26430
                     /locus_tag="PKI65_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOB77391.1"
                     /translation="MKIKIFAIALLLASIVYGQVDTPFDKKHITDSKQLKHALKNIKE
                     GNKLFGLGKGRYIDALEHFLEANKVNANNADLNYKIGICYLFTTDKELGLEHLNKAKR
                     LNPQVTYELSYYLGRAYHLNHQFDTAIVYYNEFKRRLSPKEFSIRGRELDKLIEECEN
                     GILLSTKKTKVFIDNLGPNINTIYPEYTPVISADEEVMFFTSRRPSTTGGKRDPIDNK
                     FFEDIYMATKDNDNWNLSQLGKPLNSKFHDASIGLSPDGQTLFIYKGDKGGDIYECVL
                     KGNEWSKPKPLSKEINTKYHESSASLGPDGRTLYFVSDRPGGVGGRDIWVAYKDSKGR
                     WKEVRNIRELNTPYDEEAVFIHPDGRTLYFSSKGHNTMGGYDIFKSTYKDGYWSKPEN
                     LGVPINTADDDLYFVLASSGQRAYFSSSRKGTLGDQDIFMITFIIDKPMNPSSEDNLI
                     AHKTKPVQEIIIETTIEIETPSLTLLKGRILDEETKKPLEAQIILTDNEKNEELAVFT
                     SNAETGKYLVSLPSGKNYGIAVKKDGYLFHSENFIIPENAVYQEIQKDIYLKPIKIGQ
                     SIVLRNIFFDFDKATLRPESKTELDNLIKLMNDNPNIKIEISGHTDNIGSAAYNQKLS
                     EARAKAVVDYLVEHGIDRSRLSYMGYGFEKPIASNDTEEGRQLNRRVEFKIVEVKQ"
     gene            complement(26452..26892)
                     /locus_tag="PKI65_02735"
     CDS             complement(26452..26892)
                     /locus_tag="PKI65_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77392.1"
                     /translation="MAKKQILISDLDIKRSDKIYLLQWHISDYKAAITLQQHIGSKVT
                     QLPYLNLVIKKNKINFPIFSFNYDDDEYLFIANYINNKYLHTDFLSIDYFLIPYDDTT
                     VDNWHDIYNTLKKIPSLLAIHKGNEKTKDFISLIKNQIQLQIKY"
     gene            27139..28533
                     /locus_tag="PKI65_02740"
     CDS             27139..28533
                     /locus_tag="PKI65_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOB77393.1"
                     /translation="MKTKYLDLVDQTFDFPTDEFKVIENKLYWNDINMMDIIDQYGTP
                     LRITYIPTISKKIQNARRIFNIAMAKLDYKGEYFYAYCTKSNHFSFVLEEALKNNVGI
                     ETSSAFDINIIEKLYDQGLLPKDRFIICNGFKRKEYIENITRLINNGFENVIPVIDNK
                     NELFEYRIKRNTKVKLGIRIAAEEEPTFEFYTSRLGIRYNDIVPYYKLYLKNHPNFEL
                     KMLHFFINTGIRDNAYYWNELAKAVNVYVELKRECPTLDSLNIGGGFPIRTSLNFDYD
                     YEYMAEEILNQIKTICNQNNVPEPNIYTEFGIYTVGESGAVFFNILDQKQQNDRELWY
                     MINGSFMTTLPDTWALNQRFILLPLNHWDYEYQRVFLGGLTCDSRDYYNAEAHANAIF
                     LPKFTPSNPLYIGFFHTGAYQEALGGYGGLQHCLIPAPKHVLIYLDENKEYYTKLYAK
                     EQSYKSMLKILGYM"
     gene            28680..29312
                     /locus_tag="PKI65_02745"
     CDS             28680..29312
                     /locus_tag="PKI65_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77394.1"
                     /translation="MKKLFLLCFIISSVIFTSCLKIEEYISINNEGIGSYKIKLDLAN
                     IAMFHQLMNSPTDRLAEEIQYYIDKIRAVKGISNVNFLHSEKYIEISFDFENTSAFRR
                     VLLSLTDEHFNIFIPNYIKISNNKIVKKNISPYIDKIIQYINMQNSNDFLINDYILGF
                     INFNTYILTPDSIKKISHPLAQQSKQNEVVLKTTLRDLKYGFNNKIKVKF"
     gene            29331..30446
                     /gene="dprA"
                     /locus_tag="PKI65_02750"
     CDS             29331..30446
                     /gene="dprA"
                     /locus_tag="PKI65_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOB77395.1"
                     /translation="MNDNTNILALKLIPKIGNITAKKLIEHFNDIEELFSLSSKQLQN
                     HYGLSPLLANTINKYKTEALLKAEREIKIAEQNNINVITIIEPDYPSKLKECIDAPIC
                     IFVNGEIPLLDKSIAFVGTRKASFYGREVVEKLIQEINSLSPVIVSGLATGIDTCAHK
                     AALRNDLPTIAVLGQGLSNIYPAENYELATEIIKKGGCLITEYFYDEPPLKPHFPERN
                     RIIAGLSDGVVVVQTKERGGALITANIAFSYDRSVFAVPGNINDPLAQGCLNLIKNYK
                     AEIITEGNDILKTLNWDLIPKNMTTTIIPFPLSQDEKTIYKFLLNEGDSSIDHICNCL
                     DLGIDIVSITLLNLEFAEIIQSLPGKRYRIAKYIKID"
     gene            30463..31449
                     /locus_tag="PKI65_02755"
     CDS             30463..31449
                     /locus_tag="PKI65_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77396.1"
                     /translation="MKINFLILLALNLIISCNNIIAQIYGCTDPLATNYNIKATNNDG
                     SCIYKDTVINITSTILLPQIIKESSGLIFWNEKFWTHNDDTDINLYSFNKTAPSEYTA
                     YSITGTKNIEWEELQQDDEYIYIGDTGNNANGNRTDLKILRIEKKSLLTSNPIIDSIS
                     FSYSDQTDFMPTGANNTNFDCEAFIVSNDSIYLFTKQWITLQTAIYSLSKTPGCHIAT
                     FKGQYNINGLITGAFYNQIPPLVVLCGYNKLLQPFLFLLYDFANFDFFSGNKRMIYLN
                     LPLHQVEAITSINLNTYYLTNEYFSFGKFSIQPMFHEIDLSFLLMNYLEKNK"
     gene            31500..32309
                     /locus_tag="PKI65_02760"
     CDS             31500..32309
                     /locus_tag="PKI65_02760"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOB77397.1"
                     /translation="MRKKLILIILIITTLTLNSQELRKVENKAFKTGEKITYVVYFDS
                     FITGKIFAAYATFMISPKIEYINGRPCYHAILSGKTFKKWDWAIYVDDRFDTYIDTIA
                     LVPWVFVRKAHEGKFKADQQINIDQFKHQAQFIDKRKNTNKVYPIDPYMHDMISAAYW
                     GRNLDFSKMRPGSIFKVKYVFEDSVYTSNVAFGGRETIKINEGKFDCYYVMPEVQVGN
                     VFKDPYPMKVYFTADDNQIPIYATCKIRIGNVRFELYSYENLRNPMKSKIN"
     gene            complement(32317..33228)
                     /locus_tag="PKI65_02765"
     CDS             complement(32317..33228)
                     /locus_tag="PKI65_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77398.1"
                     /translation="MKSKNTIFIVLFLIITHNIYPQSWRINRHAIGLNLGVTNFLGDL
                     GGGKSIGQPFIYDLDIQATRPLIGITYNYKIDKVNSLSTHLSFGYLRGDDAFTKNPIR
                     NNRNLNFRSPIIELTEQYELILYNSLKGRETKRYKLRGVKGWTNYEIYAFVGIGLFWF
                     NPQGKAPDGKWHNLKPLCTEGQGLDPQRKPYHRLQFTIPYGVGFRYNTSREWSYGFAI
                     APRITFTDYIDDCSTTYFNNDIIRNMKGDLAAYFADPSKGEVSGQTLPGEQRGDPKDK
                     DSYFFIYFTVNYSISALQYKTNKAKFY"
     gene            33603..33677
                     /locus_tag="PKI65_02770"
     tRNA            33603..33677
                     /locus_tag="PKI65_02770"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33638..33640,aa:Asp,seq:gtc)
     gene            complement(33879..>36350)
                     /locus_tag="PKI65_02775"
     CDS             complement(33879..>36350)
                     /locus_tag="PKI65_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB77399.1"
                     /translation="PGGNPSTSTQTNPIVTYNTAGTFNVVLTAHYGLLSDSDTQTIVI
                     NPLPNITITAVPPSICVDQSTTISASGASTYQWSNGMSSSSITVSPLTSTTYSVTGTS
                     TAGCQNTGSISVNVYAKPNISATASPSSICIGGNSVLTATGANSYSWNNGMNSATITV
                     SPSTTTTYTVTGTNTSACTNTATVTVNITTSINISATASPLNICAGDNAILMATGGDV
                     YTWNNGMNGSQITVSPTTSTIYSVTGSSGGCTGSATVSITVYPLPDISVSAVPNTICV
                     GQSTTISASGASTYQWSNGMSGSSITVSPTSTTIYTVTGTSSAGCHNINTVTITVNQL
                     PTINITSNPSHICAGSSSTLTASGASSYIWSNNMSGSIITVSPSSTTTYTVTGTNSNN
                     CSNTATATVIVESMPGAGNININATQICQNEALSLNLIGYTVGSNIQWQISSDGISWS
                     DIIGATTSSYNYLPINSGIFHFRAKVSNSCGSVYSDEIIITINSLPISGIASINTSTV
                     CQNNPVLLTLSGYSPNTTIQWQISNDNVSWSNINGATTFTYNYTPNNVGTFYIRAMVT
                     NNCGNATSNTIILNVNAIPVAGNINVNDNNICQNENVILILSGQTPMTTLQWQISTDN
                     NTWFNISGANTSSYNFIGNNVGTYYFRVLVSNDCGNATSNQINIIVNSIPPTPTITMI
                     SSYPIILFSSVSTGNQWYNESGAIPGAINQTYQVIVNGTYYVRVTQNDCQSLPSNAII
                     VNNVDIQSYYNDEVIIYPNPAQDIIYIQTDKKILEVRLIDALGRMVANTDTYQLNLDN
                     ISNGVYHLVIKFDDDVIVLPVIVKK"
BASE COUNT        13368 a         5011 c         5442 g        12529 t
ORIGIN      
        1 ttatcctaca caaacaacta catactatgg aaccataagt gatactttac atggtaatag
       61 tgctactgat tctataacta tagtagtgat acctcaacct agtatcgatt taggaccaga
      121 cacatctgta tgtgaaggtg aaataataac attatctcct ggtgctggat atgacgaata
      181 cttatggagt acaggttata caaatgctga attaatgtta gatatgtctg gagtttattc
      241 tgtaacagtt gctaatactt atgatgatgt ttcatgcttt gatagcgata ctattaaggt
      301 tgtcttcgta ccaaatgtga atatagattt aggtcaagat acatgtgtga cagaaccaat
      361 tattttagat gctacccaac catatcttga catatcatat ctatggtcta cagggaatat
      421 taatcctgca attgaaatat caaatacagg gacctattgg gtaagtgccg taaattcaat
      481 atataatata tgtggtgata gcgatacaat aaatgtaaaa gtgataccag aacctatatt
      541 gtcattgcct aatcaaatta ctatctgtag tcatgaatca tttaccttat cagcatcaca
      601 atctaataac aacttatatg agtttatctg gtctactggc agtactgagc caatttatgt
      661 tttagataag cctcaagcag gtatttatga tattagtgtt aaagttatag gttgtgatac
      721 agtttacggc tctaccaacg ttatagtaga aaattgtgaa atttctattc ctaatgtaat
      781 aactcctaat gatgatggta taaatgatgt attctatatt acaaatattg aattttatcc
      841 taatagtgaa attaaaatat acaatcgctg gggaaaaaag atctatgaaa atgaaaacta
      901 ccaaggtaat tgggatggta aaggattaga tgatggcaca tattattata tctttagatt
      961 aaattatgga tacggcaaaa tagatgaata ccatggaacc attacgataa ttaaataatt
     1021 ttaaaattca aaattattgt aattcataaa aactataaca agctagttga actattatat
     1081 aatattgtaa aaaaatcatc aaataaaaca tctaattata ttaacctgca aaattataac
     1141 tgataactga caacttttaa aacagttaca atattagctc ttttgttttt ttatttaaga
     1201 ataaatatct tttaactaaa tattttctac aaaaaaatat aaatatttcg agttttaaaa
     1261 aaaaataatt aagacaaatg attaattata agcttataat tttaatcgaa taaagtgcca
     1321 aaatcttctt ctgtaatagg tattcgtttt gtagtatcta ttaattgtgc aaactttaat
     1381 tttttatttt gtaataataa aattttttct tctattgaat ctttaacaat aaatttataa
     1441 gaaataacat ttttattttg tcctattcta tgggtacgat ttatagcttg atattcgacc
     1501 gctggattcc accatggatc taataaaaac acaaaatctg cagcagttag attgatacct
     1561 actccaccag ctttaagtga cattaatatc aattttatat ctttattatt ttgaaattca
     1621 tttactattt tcattctttc ctccatcggc gtgctaccag tgagcataag aaaaggtatg
     1681 tcattagctt ctaagtaatc tttaaataaa ttcaaatgct ttacaaattg agaaaacata
     1741 acaagtttat tatcagaaac tatgattttt tgtatatttt ctattacatc attaaactta
     1801 ccactatcac tattataatt tttatctata atggcgggat gattggctat aagacgcaaa
     1861 cgcgttatac cagataaaat agaaagatat gctttatcga agcctacttt ttgctttaga
     1921 tccataagat aattgcggat ttcacttttc ttttcctcat aataactata ttgatcttct
     1981 gtcatagagc aataataagt gcgttcggtc aatggaggca actctttggc caccatatct
     2041 ttagtacgac gcagaataaa agggctaatg atttgctgta aacttttttc tgtagcttta
     2101 tgcgagtctt tttctatagg tatcataaac ttttttcgaa aagaattcaa atcgcctaat
     2161 aaaccaggat ttacaaaaga catctgcgac cataaatcta caatggtatt ttctatggga
     2221 gttcctgtaa gaacaaactt gcgattggca tttagcttgg tgataactct aaaaatttta
     2281 ctagatggat ttttgatagc ttgactctca tctaaaaaga tataatcaaa attaaattga
     2341 cttagtattt cataatcttt tctcaccgta ccataagtag tcaatataag atcgtattta
     2401 gagataatat tttgattaat tttgcgatta gtaccataat aaacgaaata agaaagtata
     2461 ggagccgtag ttttaatttc gttaatccaa ttatagatta atgataatgg cattactatt
     2521 aaaacagaaa gctttttctc tttaaaaaga gtattttttt caaatttttg agtagattgt
     2581 ggtatgagtt tttgaatttc atcttttttg tatgctaaaa atgctaatac ttgtatagtt
     2641 ttacctaagc ccatatcatc acttagaatg ccaccaaaat cgtgttgatc taaaaactcg
     2701 aaccaattaa cacctttttg ctgataaggt cttaatgttt tcctaacaga aggaggtatc
     2761 tttacatctg gtaactgatc taattttaat tgtttaacat ctgcatctat ttgtgattgt
     2821 ggagtattga tttctttaaa taattttaat tgtgttttag taacttttac attatttttg
     2881 cctttttgag ataaaatagc caactcttta tattgagcaa accattccct gggtaataat
     2941 gctactctac cgtcaggcaa aacaaaatct ctgcgatttt ctaaaatatt atttcttaat
     3001 acataaaatg gtatacaata gtcatcgaag catacttcta tttgcaaatc gaaccaatct
     3061 attccgctct tttttatatg ttgcttcagt tctactttac ctaaaaaata tttttcttta
     3121 aatgtttttg tatctaattc gatattttta ttagcaaaaa gctcactatt taaatttata
     3181 aaattaatgt aatcatataa agtttcattt tcgtttattt cattattttc ttcattgaga
     3241 tctattaata taaaattatt gtgaaattta tgtttcaatc caataaaatt caaataatca
     3301 actatttcat tttcaaattc cttatttctt tttactttag atatattaat aaaagcatct
     3361 tgtacttcat aaaaaataaa tacttctttt ttatctccat aaggtatttc ttcatttctg
     3421 taatgaaaaa ataagctcaa taatggtttc atatccattc ctagctctaa acgcaactca
     3481 gattttggta tttcttgcaa ttcattaatt atgaaatcac catttaattt tactgttagt
     3541 tgcttttcta ataatggttt tataaatttt tcaatatatg ttttttcatg aggcttagga
     3601 ataaatattt gttgtttatc taaaaatggt tcgatttttt taccatctat acctggttga
     3661 gttgtataca aaatactttt atgtatcacc cagcacggtt cataatttaa tataaaagtg
     3721 tctgacttag atagctttat atcattatcg cctacattag ctttcaaact atatttaatg
     3781 ccatcatcac ttctatcaaa aataaaaata atatctaatg acttggagct aaaatgaata
     3841 ggctcttcat taatgattac attatctctt tcgcccttat aataaagggg aatattattt
     3901 ttttctaaaa tttctaaaag tttatctgtc ttatcacgta gataatttct aatgatctca
     3961 aaagttttat catttatcgt agcaagatct tcttcaaaag taagtggatt aaaatgaaaa
     4021 actttagcaa tgtgtaagta agtcatcata gagcataatt caagtgcttt tcgagtagct
     4081 tcgtctatct caatagtaac attttttaat gtaataggct tagaagccgc actgaattga
     4141 ccatttttta aaatggatac ttcatacggt actaccatcc aatcaaagta cttatctttt
     4201 actattgtaa atattattct tctctccaca gtttttatat tcttttgcaa atataataaa
     4261 tatggactta ttaaacaaat tttttaatta aaataagggg ttaaacgata gtattttaca
     4321 aaaaagaaag atttttattg cttttaacta atatctatat ttaacaaata tctaaattaa
     4381 cattataaat attgcaaaag cgtctaagag ataacagatt ttttgtcagt actaaaatta
     4441 atcagtaata ttttatatat tttatcttta tgcaagccaa gctccaagat gcctttcagt
     4501 tttttccaat atattataat tttagtataa ggatgctgta taaatatttt taattgctca
     4561 attattcaat taataaaaaa aaattaaatt tgctacaaat aaaaataata tgcatcaaaa
     4621 aagcaaatat tttgcatttt taataatttt tttaatttta cttagttcat gcagaatatc
     4681 tcaaaagaaa aatagtccag aatatgtttc atatcaattt ttgtcacatt ttcaacagat
     4741 ggaatttgat gaagcggcaa aatacggcac agaaaacact aagatgctta taatgttttt
     4801 taaaaacatt ataggtatga tgccaccaga aaagcgagac agcattcaaa tacctgaagc
     4861 aaacgtagaa ataaaaaaat gttacgtata cgaaaatagt gctaaatgca cttatatagc
     4921 aaatgaaaaa acagatactt tagatttact taagatagaa ggaaaatggc ttgtagattt
     4981 aaaaaaagaa tctaaaaaag ctgaaaaatt ataaaaaaaa tgtaattgat tttttaagta
     5041 tcaagaatta atctgttttt aatatctcaa ttaattcgtc gattttaggt gtcaatatta
     5101 tttcagttct acgatttttt tgtcttgctt cggcggtttt agctggatct atgggggcat
     5161 attcaccacg tccagcagca gtgatacgag atggatttat atttcctttt tgtaataaaa
     5221 ttttgacaac agcagtagct ctcataacac ttaaatccca attatctttc acttcacccc
     5281 tgcctctata tggcacatca tcagtatgtc cttcgactat aatgctaata tctggatttg
     5341 cttccaatac tttagctaat ctagataatg cctcttcgcc ccttttatcg actgcccagc
     5401 taccagaggg aaacatcaaa ctctcctcta atgatacata tattttacca tctttcatta
     5461 ttacatttag tcctttatct tcataatcaa ataatgctga tgaaatcttt tttttcaaat
     5521 catttaaagt actatctcta cgtactagca tagattctaa ctctaaaatg cgtttttctt
     5581 tttcttttaa ttcttgctct aattcatcta atgtttgttt ttttaaattt aattcttgct
     5641 ctaattttcg taattgcgat tcttttaatt ctaattgttc tttagttaat gacaattcac
     5701 tactaatcaa tttagtttca tcaatattag tttgtaataa ttctttattt ttttgatcta
     5761 agatatcata gagttgaagc aatttatcat attcattttt tagtttacgt agagcgattc
     5821 ctgtatttag tgtatcttgc tttaaaaatt ttatttgttt ttctaaatca attatctttt
     5881 cttctaattc attgttttta ttctctaaag aggcacaaga ttgttgtaat ttttgtaaat
     5941 tagcttcggc ttgttctctc ttcaattttt catcttcata ttgacgtgaa ggcacacaac
     6001 tcactgtgaa tactattact aatataaata aggaaattct ttttatcata ttaaaactat
     6061 taaaattttt tacaaaatta aattaaattt tttattgaat caatcattta agataaaaaa
     6121 taataaattc aaagctttat aattattttc cttttcgatt tgctaatatt aaatttgcaa
     6181 aaaattttaa ttatgactta tgtaacaaaa gaaaaattat tttcacgaga atggtttaaa
     6241 gcctacagtt atatactgat tggtacgctc acaatggctg tgggttttgt aatgtttata
     6301 tcgccatata gatttgcgcc aggtggtgta tatggtatat cgataatttt acatcattta
     6361 ttcaatttcc ctatcggcat tactggttta tgtatagata tacctctcat attgattggt
     6421 atgaaaattt taggagctag atttggtgtc aaaactatag tcggttcatt atctttgtca
     6481 ggatggatat cattaataga atttacgtgg ggatatgatc ctcttttaaa aaacgatcca
     6541 ctagtttctg caatatttgg tggtatactt ctgggtatag gattaggatt gatattcaaa
     6601 tctaaagcta cctcgggcgg tacagatatt attgctatga tattaaataa atatactcga
     6661 atgccaattg gtcaattatt aataattata gattctatta tagttttatg cggccttgta
     6721 gctttcgaag attggaaaat tcctttttat tcttggatta ttatttatat aactggcaaa
     6781 gtagtagata ccataatagc aggtgctagt tatgaaaaag cttgtctaat aatttctgaa
     6841 aaaattaatg aaattaaaaa taaaatacta tttgatttag acagaagcgg gagtatcatc
     6901 tctattaaag gattatacac tggtactaac aagcaaatga tactaacagt agtaaatcgt
     6961 agagaagtgg aaatgttaaa aagttatata aaacaaatcg atcctgatgc tttcatgaca
     7021 gttataaacg caagtgaggt tatcggacaa ggatttaaaa gtattgatga agctataatt
     7081 aacgattaaa aactacatcg acaacatcta tttttagtat gtataaatta aaaatttatc
     7141 tgagattagt tgtgtgaaaa atcagcaaat tcaaacaaat ttaacttttt caaaaaaaaa
     7201 tgatcatata attattttat ttttataaca ataacactct tacatattat atttaaacgc
     7261 ataattcaaa gttatattat ttcaaattaa aataatataa ctttgcaaat aatttatttt
     7321 tttatgcaaa aaaatttatt ttttatttta tttttactca ttacattaat taatattgtt
     7381 catgctcaag agggcaaaca atcctcacaa atatatgggg attttcagtt agatgctatg
     7441 tattatttga cagattctac tattagtgct tatcagccaa aagaaaaact tggcaataat
     7501 gcttatttat cattatttta tgaatatggt aatatatctg ctggacttag atttgaaagt
     7561 tttttgccac cacttatagg ctatgatcct cagagtaaag gtatcggcat agctcataga
     7621 tatatagcct atgaaaccaa aaatagagat ctaaaaataa aagcgggtca tatatatgag
     7681 cagtttggcc taggtttatc tctgcggacc tatgaagatc gctccttagg tattgataat
     7741 gctatagatg gtattgaggt gatttattct tatcagcctt actttactat taaagggtta
     7801 ataggtactc aaaaattttt ttgggataaa actggcaatt atattagggc tattgatggt
     7861 gaaatatatt taaataaatt aaatcaaaaa tgggcagaag gcagatggaa ctttactcta
     7921 ggtagctcat ttgtaagtaa atacgaagaa gataaagatc ctatttataa attacctgaa
     7981 aacatcggta tttggactat taggtatcaa ttgtcatcac attttttact atggaatact
     8041 gaattagctt taaaatctca agatccatca aaaacaaata attacattta tagagaaggc
     8101 ataggagctc atacagatat ttcttttttt ggtaataact ggggtatcta tttggctgca
     8161 catagaatgg ataatatgga tttcagaatg ctacgaaatg agtctgcgac tcgcagtata
     8221 atcaactata tacctgctct cacacctcca caaaactata ttttaataaa aagctatcct
     8281 tatgctagcc aatctaatgg tgaatgggct ttttcaggac aaggtaattt tacttttcct
     8341 aaaaatactg ctttaggtgg taaatatggt actcaattac aaattaatta ttctcgtatt
     8401 cataatattg atcataagca aataaatgat actaccccat ttatgtcacc tggtactgat
     8461 ggttatagtt cagaattttt taaaattgat aaagaagtat ttttcgaaag tatagatttc
     8521 tatatagata aaaaaatcac taaagatttg aaatttaata ttttatataa ttatactgtt
     8581 tacaatagct atataataga aggtcatgat catggtacat tttatattca taatggtgta
     8641 attgatttaa attataaatt acccaaaaaa aatagtttaa aaattcaatt agctcatgag
     8701 tacaaagagc aatataacaa aaattggtta ggtgcattga tagagttagc aatatccaat
     8761 aaatggttta tttcagtgca agatgaattt aattatggga atccagacaa agaccaccgc
     8821 aaacattatt atttagctaa tataacttac ttaaaaagtg ctactcgtat ctctttgtct
     8881 tatggcaaga tgcgtgaggg cataatgtgt gtaggcggag tgtgtagatg gacaccagct
     8941 tattatggtt ttggtattaa tatttctaca acattttaaa tttaagtagt tatgaaaaag
     9001 attttttata tcattagtat tataatactc tacatttttg tgagttgtga taaggtagaa
     9061 ccgccataca tagaaaatcc tgtaataatt gacactaaca ataatgaagg gacaaaacga
     9121 gtattaatag aagaattcac tggacatctg tgccctaatt gtcccgaagc cgcacatata
     9181 actaaagttt tagaaaataa ctatcctggc aaaataactg taatagctat acatgctggt
     9241 aattttgcca tcccaatatc ccctaactat atgacagatt tcagaactag tgcaggtaat
     9301 attttaaata attcgtttgg agtttatact tatcctagcg gcatggtaga tagaatatca
     9361 gtactaggta gtcaattaat atctccaaat gattggggaa atattgttaa tgaaagatta
     9421 gctcggcaac tacaaatcga tttgaagatc acttcacagt ataatgaaaa taatagatct
     9481 tttcaatgta gagttgaagc taaaacttta gatgtaataa caaatccatt gatgcttgtc
     9541 gctcttttta ctgaagatag tataatagat tatcaaaaaa ataataaccc cctttatgga
     9601 cccactcctg atattccaaa ttatacacat aatcacgtgc taagagactg catcactaat
     9661 gtctgggggg atactttgtg tgataactgc attgttaata atgtttatta taaaaattat
     9721 tctttcgaaa tacctaataa ctggaatgct gattttgtta atttagtagt atattgctat
     9781 gattctgcaa cccaagaagt tttgcaagtt atcgaaaaac gaatcaaata atcataaatt
     9841 ttcatagcat ttattttatt attgttatta aagttttttt ttagaatttg attttttttt
     9901 taaatattta tctctttaat ctatattaaa atttattcta tcacttaatg taatattttg
     9961 taatttcgaa ttaaaaaaaa atatatgagt gcttttatta taaccattat ttttacttta
    10021 ataatttctt ttatttgctc tattatggaa gcagccatat tatctgttac accaagctat
    10081 ttagcatatt tattagcaaa aaaacctaat caaggtaaaa aattagaaaa attaaaacaa
    10141 tctattgata gaactattac tggtatttta actatgaata ctattgctaa tactcttggt
    10201 tctgcactta tcggtatgtt tgctgcaaag atttggggta gttcagctgc tggcatagtc
    10261 ggatttatta tgactttcct tatactatat ttttctgaaa ttatacccaa aactattggt
    10321 acatattatt ggagaaaaat tttgccctgg atgataccag tattattatt tttacgtaaa
    10381 ttaacttatc ctttttattt attagctcag ggcattatga aacttattat tcctaagaat
    10441 gaaccctctc atactataga ggaaattaga gcattgacac ttttaggtta tcaaaaaggt
    10501 attttagata acattcaaac ccaagtgata gataatactt tgaaattaaa agaactacct
    10561 gttcataata ttatgactcc tcgtacagta accgttgtat tagatgagaa tctaactgta
    10621 ggtgaattaa taaatctgga tgattttttt aaatattctc gcatacctgt atattcagaa
    10681 aatattgata atatcactgg atatgttttc aaaaatgatg tcttaaatgc tctgattcat
    10741 aaaaatgaat atacaaaatt agctgagatc aaaagaaaaa ttattatcat ttatgaattt
    10801 tttagtataa aaaaagcctt agacgaacta accaaaaata acgaacaaat agctattgta
    10861 atagatgaat atggatcgtt tgttggtata atcactatcg aagatatagt agaaactcta
    10921 ctaggaatag aaataataga cgaggctgat caatataaag acctacagga atatgctaaa
    10981 gagctctggc aaatgaaaat gaacaaatta ggaatcttta aatctgaaga agaaaatcaa
    11041 aataacccag acctataaat ttttttcata aaattatttt attattttcg tataaaaata
    11101 tttttatgca acaaataata ttgatcgata gatatcgaga gcatctatta cccattgtct
    11161 ttaccaaacc tattgctgaa attagagttg gtataatgac tataaaagaa aaatgggaaa
    11221 aatatactaa tgcaaaagta gacattttgt cagctgatta tttgaaaaat aaatttccta
    11281 caccagaaac tgatcaagat actatatgga ttgatgctcg aatattaccc aatagcaatt
    11341 tagctagaac ggttttaaat atcgacaaaa aacaattatt aatagataca aatggcgatt
    11401 ttgtagcctt gagaaataaa gaatttctaa atatgctgca caataatgaa ctagatagtg
    11461 gcataaatca tgttatttat caagatgaaa ttgaattgat aaaaaaaact tgggatatct
    11521 ttacacacaa tggcaatgaa ataaaaaaag attttttact acttacaaat ggaagagact
    11581 cagccaaaat tccagattac gtttatgcaa ctaaagtaga aaatattttt ttagaacctg
    11641 gagttaaatt atcgccttgc tcccttgatg ccgaagatgg accaatatat ttaggcaaaa
    11701 attctgaaat tctggatttc gctactatca agggaccttt tgcaatttta gaacattcac
    11761 aaataaaaat tggtgctaaa atatatggta atagtacgat aggacctcag tgcaaagtag
    11821 gtggagaaat aaataatgtg gtttttcaat cttatagcaa taaagctcac gatggattta
    11881 ttggtaatgc atacatagga gaatggtgta atataggtgc tgatagcaat aatagtaatt
    11941 tgaaaaatac ttatgaaaaa gtgaaaatgt ggaattatgc tattaatgct tacgaaaata
    12001 caggcttgca atttgccgga cttgttatgg gtgatcatac caaattagct atcaatacta
    12061 tgttaaatac tggtactgtg ataggatgct catgcaatat atttggagct ggtttccctc
    12121 gtacatttat accttctttt agatggggag gcgcaggtgg ttttagagtt tatgatttcg
    12181 acaaagctat atctaccgcc agtaatatga tgaatagaag agataaaaaa ttatctgaaa
    12241 ctgatattga aatattaaaa tatgtttttg aaaaagaaac ctgaataaat aaattataaa
    12301 atatggttaa aatataatat tgtaatatta tattaaaaaa tcaagaaaat tacatttaat
    12361 tttggccttt tatttgctca attatagaat aattatattt ttaaaaaaat taagacaaaa
    12421 tgacagaatt agaagaatta atgaataata tatcattttt tatgagtgat ggcggcgaag
    12481 atttttttcc agtaatatct gctgatgatg aaaaacatat aagcagcgaa cgcgtaccga
    12541 aggaattacc tattattaca ttgagaaatg cagttttgta tcccggtagc ataatgccta
    12601 tcactattgg tagagacaaa agcataaaac taataaatga tgttagcaaa actactaata
    12661 tcgttggcgt attagcacaa aaagatgcca atgtagaaga tcctactttt gatgatttat
    12721 atcaaatagg aactgtaggt aaaattatta aaattttgca aatgcccgac catactaaaa
    12781 tggttatttt gcaaggtcgt aatagattta aagtcgtcga gcctatcact actgaaccat
    12841 atcatattgc ccaggtgcaa aaagtaaaac ctattagcag acctattgat aatgctgaat
    12901 ttttagctac catagattta ataaaaaatt gtgctaaaaa attaattaaa cgatctccta
    12961 atattcctga tgaagctagc ttagctttag ataatattca aagtccagtt tatttactat
    13021 attttgtttc tactagtctt aatttgccta ttgaaaaaaa acaacaaatt ttagaaagtg
    13081 atgatgcatt agaaatagct aatatagttt tagaaaagct tgctagcgaa attcaaattt
    13141 tagaaataaa agaaaaaata gaagaacgta taaaatctga tattgataaa cagcaaaagg
    13201 attacttttt gaatgttcaa ttaaaagcta tccaagatga gttaggtgta tctactaatg
    13261 agcaagaatt aaaagaatta tatgaaaagt ctaagcaaaa gaaatggtct gatgaggtag
    13321 ctgaactttt tgaaagagaa tttaaaaaat tacaacgcat gaatccagct gctgctgagt
    13381 attctgtaca actaaattac ttagaacttc ttgtagattt accttggaat gaatatacac
    13441 aggataatta tgatcttgaa agagcaagaa aaatacttga tgaggatcat tatggtttag
    13501 aaaaagtgaa agatagaatt atagaatttt tagctgttac taaactcaat ggtaatatgc
    13561 gttcgcccat tctgtgtttg gtagggcctc caggtgtggg caaaacttct cttggtaaat
    13621 caatagctcg cgcattggaa cgtaaatata ttcgtatttc actaggaggt ctacacgatg
    13681 aggcagagat tagaggccat agaaaaactt atgtaggtgc tatgccgggt agaataatac
    13741 aaagcttgag aaaggtacga gccgctaatc ctgtatttat gcttgatgaa attgataaag
    13801 tcggtagcct ctcaatgcag ggcgatccac aagcagctct attagaggtt ctagatcccg
    13861 agcaaaacac gcatttctat gacaattact tagaaactga atttgacctt tctaaagtta
    13921 tgtttatagc tactgctaat accacagcta ctataaatcc tgctcttctc gatagaatgg
    13981 aagtaataga acttcatggt tatgtattag aagaaaagct agaaatagct aagcgctttt
    14041 tggttcccaa acaactcgaa gaaaatggaa tggctgattc taaactaaaa ttttctgaca
    14101 atgccattag agagattata gaaaattata ctcgtgagag tggagttaga gctttagaaa
    14161 aacgaattgc taaagtgata cgtaataaag ccgtagactt cgcttctggc aaattgaaaa
    14221 aaactacttt aacagttaat gacattcaaa atatattagg agttcctatt tttaaacgag
    14281 aaaaagcttt aaatcaaccc acaataggcg tagttttagg attagcctgg acacccgtag
    14341 gtggagaaat cttattcgta gaagcatcta cacataaagg agatggtaag ttatctatga
    14401 ctggtaatct tggtgatgtg atgagagaat ctgccactct agcttttgaa tttataaaag
    14461 ctaatgctat atcgctagaa ttagacgaaa acttctatca aaaagttaat atacatatac
    14521 atgtacctga gggtgctatt cctaaagacg gcccatctgc aggtgttacc atgcttacag
    14581 ctatggctag tgtattactc aatcgtgccg tacgtaatga tatagctatg acaggtgaaa
    14641 tcactctgag aggcaaagtc ttacctgtcg gcggtattaa agaaaaaata ctcgccgcat
    14701 ctagagctgg tattaaaaat ataattttac ccatcgataa taaagctgac ttttacgata
    14761 tagaaaaaca ctatactaaa gatctaaacc ctatctttgt agaaaatatg atagatgttt
    14821 taaaagaagt tttagttaaa gattaaggtt ctataacgtt tattgtgggt aaaaacattt
    14881 tatattgtta caaaaaaata actatttttt aattatttag gttatttttt taccacaaag
    14941 ggcaccaaga tgatacactt tgtgtattct ttgagaactt tgtggttaat atttgactat
    15001 cctataatta ataaactaat attgacaaat ttatttttgt aaaattattc tttttttatt
    15061 ctttacccat ataaaacatt atagaaacaa aattaatatg tatttatagg tattatatta
    15121 aaacatgtta gttccattat tttccatttc atctatatta ttcagatgtt tttctttata
    15181 atttccaaat agcttatatt taatatttat tcttatcccc aagcctccat gataattttt
    15241 tatcaatgaa taataagtcg gttgataata ttccattttg aaatgagctg tgttaaataa
    15301 atttctaact gtcatgctta gttctaatct atcccaggat ttttgcacag aaagatgtga
    15361 atttaaaaat ccacctcttt tgccttggcc agtttctgtt ggcaatctat acattatcat
    15421 ccattggata ttccaatttt taggaagttt aaattgattg tttaatctag caaaacctcc
    15481 ataagactca cttttcacac tagcatctac atttgaacca tctatcacat tataatgaaa
    15541 tacagtgttt aacataagat tccaataatc ggcaattcta tattgaaagg ttatttctaa
    15601 tccataagag ttagaagatt tgaaattttc gaaagttgta agatatactg tatcatttag
    15661 ttgcttgata taactactaa tcatatctgt agttttacga taaaaaatac ttggtgctat
    15721 taatcctttt ttaaaatatt gcataaatga tatttcaaat gagtttatat attgaggttt
    15781 caaaaaagga tttccactac taatgtacgg gtaagatgaa taatcaatat agggatttaa
    15841 gtagccaaac caaggtctat aaactctacg actatacgaa aattgaattt gttgcttttt
    15901 attgatatta taagaaatgg cacctgtagg atataaacta taatacgtat ttttattatc
    15961 agtagaatca gcttgtctag cattagtatt tgtcatttca cctcgtaaac ctaatgagaa
    16021 tccccatttc ttaattttag tattaaacac tgcatacaaa gcattcacat attcatcata
    16081 aagaaaatga ttactttgca tggaatctag taagtaaata ttttcattaa aaagatattt
    16141 gtaatatctt gtatccatat cgtatattct gagattagat tcaaatccac cctctatctt
    16201 tgtactatca ttaaaagggt atgaataatc tatctttaaa taaccttgta aattatcaga
    16261 gatactaaaa gttttgttcc aaaaaggttg catagtttct gatggagtag tataatctga
    16321 ttcgaaataa tttcttttta ttatgttcat tctaggagaa tatgatggat ataacgaagc
    16381 attaacatct aatttatgat ctttcttggg aaatttatga gtataatata aagcaaaact
    16441 catattttga gaagtatcat tagatgttct tataatattg tatatctgtg ttggattttt
    16501 taaagtatcc aaatactcag cccatgtggt agattcacca ctatttaaac gatatctata
    16561 tgcagcataa aaaccaagtg tattattatt gtttggtctc cattctatag ttccttgcaa
    16621 gttatgattc cacccgccat gatttccata attattttga tttaggtaat gcatttcatt
    16681 taaattataa ttttctgtat aagtactacc tttttcataa tgtttattat atctaggaga
    16741 atattgtaaa tttacagtaa atttcttagc atcataagag atagcaaaca tggcagaaaa
    16801 tggtgataaa aaaggtttaa atttagaaaa atcttcgata ttttcgttca tcataccacc
    16861 agaaacgcgt atcatactat ttagtccaaa acttttttgt ttcttaagtt gtaaatttaa
    16921 aataccgctg gtaccctctg gactatattt tgcagaagga tttgtcaata tttctatatt
    16981 ttctatttct tctgcattta tttgttctaa aatagtttta ttatcaccta aaagtgcaat
    17041 tggacgatta tctactaaaa tggttacatt ttgactacct cttagactca cattaccatc
    17101 ttcatctacc tctactcctg gcacattttc taaaacatct aaagctgtgc ccccttttga
    17161 tgtcaatacc tgatctacat tgatgattcg tttatctgct cgtagctcta tcattggctt
    17221 ttcagctact acttctatac tttgcaataa ataagccgaa ggtctaagct ttatagtacc
    17281 tatattaata ttaaaatttc cactacgagg attcaaaatt atatttggta tgattttatt
    17341 agcatatcca acaaaactta cttggatata aaaattgcca atacctttgt gttttaacac
    17401 aaaattacca tttatatctg tagtagtccc atttactaat atactatctt tagtttgata
    17461 taatgcaata actacaaatt ctaatggatt atttgataat gaatctatta ctttaccact
    17521 tatagtgaat tcaggcattt cttttgatgt tacattttga gatgtattct gtgaataaac
    17581 acacgaaatt aaaagtataa atgcagttaa tagcgcaaac aattttttca tagttaattt
    17641 tttaaattag acaaaattaa ttaaaaaaac ttgtgttttt atcatattat tttaaaaaat
    17701 gtattatttc ataaaaaaat actttaattc aaaaaatata tttaattttg tcgttaaaat
    17761 atatggatat gttaaaaaat tatattaaga cttctaattt tacaaagaaa attagaaata
    17821 attttattga aagtttatat ttattatcta ttttattatt tacattaatt tttaatacta
    17881 atcttttttc acaggatatt gctttttcga gcaattattt tcctaataat ccaggattaa
    17941 aagaagctaa aaaaaatata aaaaatggta attcattgtt tataaaagga gagggatatt
    18001 atacagaagc tttagaatat tatttaaaag ctaatcaatt taattctgat aatgctgaac
    18061 taaattataa aatcggtatt tgctatttat attcagctga tatagaaaaa gctttagaac
    18121 acatagaaaa agcttataga ttaaataaaa atgttaatga tgacataact tattatttgg
    18181 ctcaagcata ccatttgaat ttagattttg ataaagctat cttatattat caaacatatt
    18241 tgaatgtttt aaagcctcgc gatagagata aacatttaag taaaattaat aaaaaaattg
    18301 aagaatgtaa taatggcaaa gaattaataa aagatactgt aaaagtattg atagataatc
    18361 tagggggcac tgttaatagt atttatccag attatgctcc agttatcagt gcagatgagg
    18421 ctatgttgat atttacaagc cgcagacctg gtaatatagg gggaatgcga gatccaataa
    18481 atggaaaata tttcgaagat atttatattg catatatgga ggaatggggg aaatggctat
    18541 taacacaacc aagtgcgcct ctcaatgata aatatcataa tgccgcaatc gggctaagtc
    18601 cggatggaca aactctctat gtgtacaaag gggtaaatgg tggcgatatt tatgtttctt
    18661 atttgaaagg gaaaaattgg acaaatccag aacctctacc ggctccgata aattcaaaat
    18721 atcatgagtc tagtgcttct ctggggcctg atggtagaac attatatttt gtttcagata
    18781 gacctggcgg tataggtggt agagatatat ggatggcacg tatggataat atgggcaatt
    18841 ggacaaatcc tatcaatctc tcgagtataa atactccgta tgacgaagaa ggggttttca
    18901 ttcatcccga tggtagaact ttatatttca gttctaaagg gcataattct atggggggat
    18961 atgatatatt tcgatcagtt tatcaagatg gtaaatggag taaacctgtc aatttaggtt
    19021 atcctattaa tactcctgat gatgatttat attttgtttt gtcagcatct ggtatacatg
    19081 cttatattag ttcatctaga aaaggtacgt taggagatca agatatcttt atgatcactt
    19141 atctcatagc taagcctgtt atgcctagta gcgaagctaa tttattggct tggcgtggca
    19201 aacctagtat atatgctact ggtagtcaat ctttaatcgc cgaaaattta ccatctatga
    19261 ttttatggaa aggcaaagtt attgatgaag aaaataaccc tttagaagct caaattatcc
    19321 ttacagataa tgtaactgga gagcaattag cagtatttac atctaatagc gaaactggta
    19381 atttcttagt gtcattacct agtggtaaaa attatggtat agctgttaaa aaagaagggt
    19441 atttatttca ttctgaaaat tttaatatcc cagaaaatgc accttatgaa gagttaaaca
    19501 aagatattat acttaaaaaa gtagctgctg gcgcagagat agtattgaat aatatattct
    19561 ttgattttaa caaagctact ctgagacctg agtctaaaac tgaattacaa aatgtcattg
    19621 aatttatgca acaaaatcct agtgtaatta tcgaaatatc tggccatact gataatattg
    19681 gcagctatga atataatcaa agtttatctg aaagacgtgc taaagctgtc gttgattatt
    19741 tagtagaaca tggtatacct ttatctaggt ttgtttataa aggttattct ttttccgaac
    19801 caatagctac taatgatact gaagagggtc gtcaattaaa ccgtcgagta gagtttaaaa
    19861 tcttagcagc agatgaggtt tataaatcgg atacttatat tcaaacagct aaatctgaaa
    19921 caaaaacaat aaaagctgaa gtacaaccca tagaaactac taaaactact aaattaattg
    19981 tagaggaaga agttaaaaaa gtagaacctg taaaagaaga atttaaaaaa gaagtgcctg
    20041 caaaagaaat tattcccact aaacctacac cacaaaaatt atttcatata gttggtgcta
    20101 gtgtaacaaa tatcaatgat gctgaaaaaa taaaaaatga atatattaag aaaggataca
    20161 tagatgctac aatactacct agatcagatg gtaaaggata tagaatagct tataaaagtt
    20221 ttactacaaa agaggaagcc ataaaggaat tagaaatatt aaaaaaagaa accaaacaaa
    20281 aagatctctg gatacttcag caataaataa aaaaatgaaa tttaaaacaa aaatataaat
    20341 ctatgaaacg tgaattaatt ttaagtttaa tattaacttt tataatgagc attgttaatg
    20401 ctcaagctgt aaaagttggt gaatctcaga tagcagaaca gatgtgcaat caaggagtta
    20461 tgctgctaga ggctggcgac ttacaaagtg ctattgctaa atttaatgat gctatatttt
    20521 taaaccctaa ttatgctata gcttattatc atagagccat ggcaaaatca aaaataggtt
    20581 tgtatgatga tgcattaaat gatttaaata aagctttaaa tattgaagtt aatggaatgt
    20641 attactatag cagaggtatg gtgaaacaag aaaaaggaga tttccaagga gcattacaag
    20701 attataacgc tgctataaat acaggtcaaa acttagctga agcatactat tatagagcat
    20761 ctctatactt cgatcaaggt gattataata gtgctattga tgatctgaag caagccatat
    20821 ctaaaaaaga taattttgca tatgcatata atgacttggg tagttgctat atgatgttag
    20881 aagactacga taatgctatt ttctattaca atcaagcaat taaagccgat cctaaatttt
    20941 caatagctta taataactta ggcattacat ataaaaatat gaaaaaatat gatgaggcta
    21001 taatggaatt tactaaggca atagatttag atcctgatta tgtgatagca ttaaataata
    21061 gaggcgttgc taaattagaa aaaggtgatt ataaaggagc cgctgatgac tttacattag
    21121 taatacaaaa agatcctgat tattattttg cttataataa tcgtggcatt gctaaataca
    21181 aaatagagga ttatcagagt gcacttgaag atttcaataa agctattgaa ttaaatgaaa
    21241 attatgcagt agcataccta aatagagcaa atactaaaga aatgcttcgt gatatggatg
    21301 gtgcatgcaa tgattggcaa aaagctggtg agtttggtat caaagctggt gatatgtatt
    21361 ataaacaaat gtgtaataaa taaaaattaa aatgttatga aaatgttatt aaatacccat
    21421 aagattaaaa aaaaatcata gccactgtaa atgaatattt ttatgtaaat ttgtttaaaa
    21481 aaaattatat acagatgaaa ataaaaatct ttgctatagc tttattatta gcatccatag
    21541 tatatggaca ggtagatact cctttcgata aaaaacatat tacagatagt aagcaattaa
    21601 aacatgcatt aaaaaatatc aaggaaggaa ataaattatt tggcttaggt aaagggcgat
    21661 acatagatgc attggaacat ttcctggaag ctaataaagt aaatgcgaat aatgcggatt
    21721 taaattacaa aattggtatt tgctatttat ttacaactga taaagaacta ggattagaac
    21781 acttaaataa agccaaacga ctaaatcccc aagtaactta tgaattatct tattatttag
    21841 gtagagcata ccatctaaat catcaattcg ataccgctat tgtgtattat aatgaattta
    21901 aattaaattt atataaacgc gaacttaaaa ttagagcctt tgaaatagat aaattgataa
    21961 atgaatgcga gaatggtaaa gaattagtaa aaaaatcaga aaaattatta attgataatt
    22021 taggtccaaa tattaatact atctatccag aatatgcacc aataataatt gcaaacgaag
    22081 aagttatgtt ttttactagt cgcaggccaa ctactactgg tggcaaacgt gatcctatag
    22141 ataaaatata ttatgaagat atttatatgg ctaccaaaga taatgataat aattggcaac
    22201 agatgcctgt aacagaacta aatacaaata aacatgatgc agttgttggt tatttcaaag
    22261 gtaaagagtt atcatattta tatacatata aaaataagca cggcggcgat atatatatat
    22321 cattaataaa gaataataaa atagatattc ctaaaccatt accattccct ataaatacaa
    22381 aatatcatga gtccagtgct tcgttatcac ctgatagcag tactctttat tttatatctg
    22441 atagaccagg tggtatcgga ggtaaagata tatggatgtc tcacaaagaa tataatcaaa
    22501 tgtggtcaga accaataaat ctttctgata ttaattcacc ttatgatgag caaaatgttt
    22561 ttatccatcc caatggtaaa attttatttt tttgttcaaa tggtcataat accataggtg
    22621 gttttgatat tttctactca atttatcaag atggaaaatg gggtaaacct ataaatatgg
    22681 gttatcccat caatacagct gctgatgaaa caaattatac tttatcttca tgtggttctt
    22741 atgcttatat aagttccaat cgtcaaggca caataggtga tatagatatt tttaaaattt
    22801 cttatacaat aaaaaaacat gttttgccta gtagtgaaaa tgaattagtt gcatggcaag
    22861 ctttatttaa aaaattgtca gaaaaagaaa caaaaaattt acttacaaaa acagaaaata
    22921 tgaaagattt gccatcacta accaatgata acttgtataa tgaaatatta tggtcaaata
    22981 atatattttt tgagtttgat tcaatcgctt taagttctga atctagaaca gtattagata
    23041 atctcataaa gctaatgaat gataatcctg atatcaaaat agaaatttct ggccatactg
    23101 ataatattgg ttctgctgct tacaatcaaa aattgtctga agcaagggct aaagctgttg
    23161 tagattattt agtagagcac ggaatagaca gatctagact ttcgtatata ggctatggct
    23221 tcgaaaaacc tattgctact aatgatactg aagagggtcg tcaattaaac cgtcgagtag
    23281 aatttaaaat catccaagta aattaacata taaaatcata aatcatgaaa cgaataatca
    23341 ttttatgtat aataggaatg gtaacaatga gttttttaga agctcaggat actattaaaa
    23401 ttattagtga taacttacaa atagccgagc aacgatataa tgaaggcgtc aaattattag
    23461 aatctggtaa tattcaagga gctattaata aatttactga ggctataaat ctaaaaccag
    23521 atttgtcaaa agcatattat aatagagggg tagctaaatc ccaagcagga ttatacgacg
    23581 aggctatagc agatctagac aaagctctta gctacgaaac aagtggattt tattattata
    23641 ctagaggcaa agctaagcac gaaaaaggag acttagaagg tgcactcgaa gattacaatg
    23701 cagctattaa ttttgataaa aatctagctg aagcatacta ttatagagca tctctatact
    23761 tcgatcaagg tgattataat agtgctattg atgatctgaa gcgagctata tctaaaaaag
    23821 atggctatgc ttatgcatac aatgatctgg gcagttgcta tctgatgtta gaagactacg
    23881 ataatgctat ttattattat aaacaagcta taaatgctga ccctagattg acattagcat
    23941 ataataatct tgggacaact tataaaagga tgaaaaaata tgatgaggct ataatagaat
    24001 atactaaagc tatagatata gatcctgact atgtaatagc attgaataat agaggattag
    24061 ctaaattaga gaaaggtgat tataaaggag ctataaatga tttcactgaa gcattaaaaa
    24121 aagatcctaa ttattatttt gcttataata atcgtggaat tgctaatttc caacttgaag
    24181 attaccaaaa tgctcttgaa gatttcaata aagctattga attaaatgaa aattatgcag
    24241 tagcatacct aaatagagca aatactaaag aaatgcttcg tgatatggat ggtgcatgca
    24301 atgattggca aaaagctggt gagcttggta tcaaaactgg tgatatgtat tataaacaat
    24361 tgtgtaataa ataaaaatta aaatgttatg aaaataaaaa tctttgctat agctttatta
    24421 ttagcatcca tagtatatgg acaggtagat actcctttcg ataaaaaaca tattacagat
    24481 agtaagcaat taaaacatgc attaaaaaat atcaaggaag gaaataaatt atttggctta
    24541 ggtaaagggc gatacataga tgcattggaa catttcctgg aagctaataa agtaaatgcg
    24601 aataatgcgg atttaaatta caaaattggt atttgctatt tatttacaac tgataaagaa
    24661 ctaggattag aacacttaaa taaagccaaa cgactaaatc cccaagtaac ttatgaatta
    24721 tcttattatt taggtagagc ataccatcta aatcatcaat tcgataccgc tattgtgtat
    24781 tacaatgaat ttaagcgaag attatcacct aaagaatttt ctattagagg tagagaattg
    24841 gataaattaa tagaagaatg tgagaatggc attctgttat ctactaaaaa aaccaaagtt
    24901 tttatagata atttaggtcc aaatattaat actatttatc cagaatatac accagtaata
    24961 agtgctgacg aagaagttat gttttttact agtcgtagac cgagtactac tggtggcaaa
    25021 cgtgatccta tagataataa atttttcgaa gatatttata tggctaccaa agataatgat
    25081 aattggaatt tatcccaact aggtaaacca ttaaatagta aatttcatga tgcatcgata
    25141 ggattaagtc ctgatggaca gacactcttt atctataagg gtgataaggg cggtgatata
    25201 tatgaatgtg tattaaaagg caatgaatgg tctaaaccta aacctctatc aaaagaaata
    25261 aatacaaaat atcacgagtc cagtgcttct ctcggccctg atggtagaac cttgtatttc
    25321 gtatctgata gaccaggtgg cgtggggggt agagatatat gggtagcata taaagatagt
    25381 aaaggtagat ggaaagaagt aagaaatatt cgtgagctaa atactcctta cgatgaagaa
    25441 gcagttttta tacatcctga cggtagaacc ttatacttta gttccaaagg tcataatact
    25501 atgggaggct atgatatttt caaatccaca tataaagatg gctattggag caaaccagaa
    25561 aatttaggtg ttcctattaa tactgctgat gatgatttat attttgtttt agcttcctca
    25621 ggtcaaagag cttatttcag ttcttcgcgt aaaggtactc tgggtgatca agatattttt
    25681 atgattactt ttattataga caaacctatg aacccctcat cagaagataa tctaatagct
    25741 cataaaacca aacccgtaca agaaatcata atagagacca ccatagagat agaaactcca
    25801 tcattgacat tattaaaagg tcgcatccta gatgaagaaa ctaaaaaacc attagaagct
    25861 caaataattt taactgataa tgaaaaaaat gaagaattag ctgttttcac ttctaatgct
    25921 gaaactggca aatatcttgt atctttacct agtggcaaaa attatggtat cgctgtcaaa
    25981 aaagatgggt atctgtttca ttcagaaaac ttcattatcc ccgaaaatgc cgtatatcaa
    26041 gaaatacaaa aagatattta tctgaaacct ataaaaatag gacaatctat cgtacttaga
    26101 aacattttct ttgatttcga taaagctact ttgcgccctg aatctaaaac tgaattagat
    26161 aatctcataa aactgatgaa tgataatcct aatatcaaaa tagaaatttc tggccatact
    26221 gataatattg gttctgctgc ttacaatcaa aaattgtctg aagcaagggc taaagctgtt
    26281 gtagattatt tagtagagca cggaatagac agatctagac tttcgtatat gggctatggc
    26341 ttcgaaaaac ctattgctag caatgatact gaagaaggtc gtcaattaaa ccgtagagta
    26401 gagtttaaga ttgtcgaagt aaaacaataa accactataa atctcagttt tttaatattt
    26461 tatttgcaac tgtatttgat tttttatcaa gctaataaag tcttttgttt tttcatttcc
    26521 tttatgtatt gccaagagtg aaggaatttt ttttaatgta ttatatatat catgccagtt
    26581 atctacagta gtatcatcat aaggaattaa aaaataatct attgataaaa aatcagtatg
    26641 caaatattta ttatttatat aattggcaat aaatagatat tcatcatcat cataattaaa
    26701 tgaaaaaata ggaaaattta ttttgttttt tttgataact aaatttaaat acggtaattg
    26761 agtaacttta gaaccgatat gttgctgtag agtgatggca gctttgtaat cgctaatgtg
    26821 ccattgtaat aaataaattt tgtcacttct tttgatatca agatctgaaa ttaaaatctg
    26881 ttttttagcc atactaacta tttattaagt atattttaat aaaaaaatat gtgaagctga
    26941 ttgctaattt tatcattaac ttacaactaa caaaaaaact ttgtttaaaa ctattgcaag
    27001 ttataaaaaa taaattatta attacaaaaa tattaaaaat acaataactt attaaattat
    27061 tttttaagat ttttttcatt gctatttaaa aaattaaagt atttttgcac atttaaaaaa
    27121 aagttattaa aataataaat gaaaaccaag tatttagatt tagtagatca aacatttgat
    27181 tttccaacag atgagttcaa agtaatagag aacaaactct attggaacga tatcaatatg
    27241 atggatataa tagatcaata tggtacacca ttacgaataa catacatacc gactattagc
    27301 aaaaagatac aaaatgcacg tagaatattt aatatagcta tggcaaaatt agattataaa
    27361 ggtgaatatt tctatgcata ctgtactaaa agcaatcact tttcttttgt gctagaagaa
    27421 gctttaaaaa ataatgtagg catagagact agtagcgctt ttgacataaa tattatagaa
    27481 aaattatatg atcaaggact tttgcctaaa gatagattta taatatgtaa tggttttaaa
    27541 agaaaagaat atattgagaa tattactaga ttaataaata acggtttcga aaatgtaata
    27601 ccagtaatag acaataaaaa tgaactattt gagtatcgta taaaaagaaa tactaaagtg
    27661 aaattaggta tacgcatagc tgccgaggaa gaacctacat ttgaatttta cacctcgaga
    27721 ttaggtatac gttataatga tatagtgcct tattataagc tttacttaaa aaatcatcca
    27781 aatttcgaac taaaaatgtt gcattttttt ataaatactg gcattagaga taatgcttat
    27841 tattggaatg aattggctaa agcggttaat gtttatgtag aattaaaaag ggaatgtcct
    27901 actttggatt cattaaatat aggtggagga tttcctatac gtacgtcatt aaattttgat
    27961 tatgattacg aatatatggc agaggagata ctaaatcaaa taaaaactat ttgcaatcaa
    28021 aacaatgtac cagaaccaaa tatttatact gagtttggca tatacactgt cggagaatca
    28081 ggagctgttt tttttaatat tttagatcag aaacaacaaa atgatagaga gttatggtat
    28141 atgataaatg gatcattcat gactacccta cccgatactt gggctttaaa tcaaagattt
    28201 atactattac ctttaaatca ttgggactat gaataccaaa gagttttttt aggtggatta
    28261 acctgtgata gtcgagatta ttataatgca gaagctcatg ctaatgctat atttttacct
    28321 aaatttactc caagcaatcc attatatata ggtttttttc atacaggtgc atatcaagaa
    28381 gcattgggtg gttatggtgg tctgcaacat tgcttgatac ctgctcctaa acatgtgctt
    28441 atctatcttg atgaaaataa agaatattat accaaacttt atgctaaaga acaaagctat
    28501 aaatctatgc taaaaatatt aggatatatg taaaaggata tatctcaaaa tcaaactaat
    28561 attgtgatat tgagtcaaaa aataaaaaag taatacttga ttgtagataa aattagtaca
    28621 ttcattaaat tgtaaacaat aaattaattt taatattttt gtaaaaaata ttaattttta
    28681 tgaaaaaatt atttttgctt tgttttataa tttcttccgt tatttttact tcttgtttaa
    28741 aaatagaaga atatatatcc ataaataatg aaggtatagg atcttataaa atcaaattag
    28801 atttagctaa tatagcaatg tttcatcaat taatgaattc accaacagac agattagctg
    28861 aagagataca atattatata gataaaataa gagctgtaaa aggtattagc aatgtcaact
    28921 ttttacattc agaaaaatac atagaaatat catttgattt cgaaaacact agtgctttta
    28981 gacgtgtttt gctatcactt acagatgaac attttaatat ttttattcct aattatatta
    29041 aaatatctaa taataaaatt gtaaaaaaaa atatatcccc atatatagat aaaatcatac
    29101 aatatattaa tatgcaaaat agtaatgatt ttttaataaa tgattacata cttggattta
    29161 taaatttcaa tacatatatt ctgacacctg atagtatcaa aaaaatttct catccattag
    29221 ctcaacaatc aaaacaaaat gaagtagtac tgaaaaccac tctaagggac ctgaaatacg
    29281 gttttaataa taaaataaaa gtaaaattct aattttctta tttagtaaaa atgaatgaca
    29341 ataccaatat tttagctttg aaattgatac caaaaatagg taatattact gctaaaaaac
    29401 ttattgagca ttttaatgat atcgaagagt tgttttcatt atcatctaag caactccaaa
    29461 atcattatgg gctatcacca ttattagcaa acacaataaa taaatataaa actgaagctt
    29521 tattaaaagc tgaaagagaa attaagatag cagaacaaaa caatataaat gtcatcacta
    29581 ttattgaacc cgactatcct agcaaattaa aagaatgtat cgatgcgcct atttgtatat
    29641 ttgtgaatgg cgaaatacct ttattagata aatctattgc ttttgtaggt actagaaaag
    29701 catcattcta tggcagagaa gttgtagaaa aattaataca agagatcaat tctttatcac
    29761 cggtgatagt aagtggcttg gctacaggta tcgatacctg tgcccataaa gctgcgttac
    29821 gaaacgattt acctactata gccgttttag gacagggttt atctaatatt tatccagcag
    29881 aaaattatga gctagctacg gaaataataa aaaaaggtgg gtgcttaata acagaatatt
    29941 tctacgatga accgccacta aaacctcatt ttcctgagag aaatagaata atagcaggac
    30001 tctctgatgg agtagtagta gtgcaaacaa aagaaagagg tggagcactt ataactgcta
    30061 atatagcatt ttcttatgat agatccgtat tcgctgtccc aggtaatata aatgacccct
    30121 tagctcaagg ttgtctaaat cttattaaaa attataaagc tgaaataata actgaaggaa
    30181 atgatatact gaaaacatta aactgggatt tgataccaaa gaatatgaca actactatta
    30241 taccattccc cctctctcaa gacgaaaaaa caatttataa attcttatta aatgaaggcg
    30301 atagcagtat agatcatata tgtaattgtc tagatctcgg tatagatatt gtttctatca
    30361 cacttttaaa tttagaattt gctgaaataa ttcaatcact tccaggtaaa agatatcgca
    30421 ttgctaaata tatcaaaata gattaattat aatttattaa aaatgaaaat caatttttta
    30481 attctattag ctttaaactt aattatatct tgcaataata taattgctca aatctatgga
    30541 tgcacagacc ccctcgctac taattacaat ataaaagcta caaataatga tggtagctgt
    30601 atatacaagg atacagttat aaacataaca tctactatcc tattgccaca gataataaag
    30661 gagagctcag gattaatatt ttggaatgaa aaattctgga ctcataatga tgatacggat
    30721 attaatttgt attcatttaa taaaactgca ccttcagaat atacggcata tagtatcact
    30781 ggaactaaaa acattgagtg ggaagagcta caacaagatg atgaatacat ttatataggt
    30841 gatacaggta ataatgcaaa tggcaataga actgatctga aaatattaag aattgaaaaa
    30901 aaatcgcttc tcactagtaa tcctatcata gatagtatat cgtttagtta ttctgatcaa
    30961 actgatttca tgcctactgg ggctaataat actaattttg attgcgaagc cttcatagtc
    31021 tctaatgata gtatatattt atttactaag caatggatta cacttcagac tgccatttat
    31081 tcactttcta aaacacctgg ttgccatata gctactttta aaggtcaata caatataaat
    31141 ggacttatca ctggtgcatt ttataaccag ataccaccac ttgtagtttt gtgtggatac
    31201 aataagttgt tgcaaccatt tttatttctt ttatatgatt ttgctaattt tgattttttt
    31261 agtggtaata aaaggatgat ttatctcaat ctcccactac atcaggtaga agctatcact
    31321 tcgataaatc taaataccta ttatctgaca aacgaatatt tttcttttgg caaattttct
    31381 attcaaccaa tgtttcatga aatagactta agttttttat taatgaatta tttagaaaaa
    31441 aataaataaa tatcacttat tggcacatat tttgaatgtc attaaataaa ttaataaaaa
    31501 tgagaaaaaa actaatttta ataattttga taattacaac gctaacttta aactcacaag
    31561 aattaagaaa agttgaaaat aaagcattta aaacaggtga aaaaataacc tacgttgtat
    31621 attttgattc atttattact ggtaaaatat ttgctgcata tgctacattt atgatttcac
    31681 caaaaataga atatataaat ggcagacctt gttatcatgc aatattaagt ggtaaaactt
    31741 ttaaaaaatg ggattgggct atctatgtag atgatagatt tgatacctat atagacacta
    31801 tagctttggt gccatgggtt tttgtaagaa aagctcatga aggtaaattt aaagcagatc
    31861 aacaaataaa catagatcaa ttcaaacatc aagctcaatt tattgataaa agaaaaaata
    31921 ctaacaaagt ttaccctatt gatccctaca tgcatgatat gatttcggca gcttattggg
    31981 gaagaaactt ggatttttct aaaatgcgac caggtagtat ttttaaagta aaatatgtat
    32041 ttgaagacag tgtttataca tcaaacgtgg cttttggtgg tcgagaaact attaaaataa
    32101 atgagggcaa atttgactgc tattacgtga tgccagaggt acaggtaggt aatgttttca
    32161 aagatccata tcctatgaaa gtttatttta cggctgatga taatcagatt cctatttacg
    32221 ctacttgtaa aattagaatt ggtaatgtaa gatttgaatt atatagttac gaaaatctac
    32281 gcaatcctat gaaaagcaaa ataaattgaa ttagttttaa tagaatttag ctttgttagt
    32341 tttatattgc aatgctgaaa tggaatagtt aacagtgaaa tatataaaaa aataactatc
    32401 tttatcttta ggatcacctc tttgttctcc aggtagagtt tgtccagaga cttcaccttt
    32461 ggatggatct gcaaaataag ctgctaaatc ccctttcata tttctaataa tatcattatt
    32521 aaaataagta gtactgcaat catcaatata atctgtaaaa gtgatgcgtg gtgctatagc
    32581 aaatccataa gaccattctc tacttgtatt atatctaaaa cctacaccat aaggtatggt
    32641 aaattgcaat ctatgatatg gtttgcgctg tggatctaaa ccttgtcctt cagtacataa
    32701 aggttttaaa ttatgccatt taccatcagg tgctttacct tggggattaa accaaaataa
    32761 tcctattcct acaaatgcat atatttcata attagtccat ccttttactc cacgtaattt
    32821 atatcttttt gtttctctac ctttcaaaga attatataat attaattcat attgttctgt
    32881 tagttctatt ataggactac gaaaatttag atttctatta tttctaatag gattttttgt
    32941 gaaagcatca tcacctctta gatacccaaa agataaatgt gtacttaaag agttgacttt
    33001 atctattttg taattataag tgatacctat taaaggtcta gtagcttgga tgtctaagtc
    33061 atatataaat ggctgtccta tacttttgcc accacctaaa tctcctaaaa aattggtaac
    33121 tcctagattt agacctattg catgcctatt tatcctccaa gattgtggat atatattgtg
    33181 agttataatt agaaataata ctatgaaaat tgtatttttt gatttcattt aataatttat
    33241 ttatgcaaat ttatataatt ttttgtattt aaatttagta ttaatttaat aaaatatttt
    33301 accaattatt agagctaatt atcataacaa gtacagaatc ttattttcaa ttaactaatg
    33361 catcactaaa aaccattcta aattttaata attttgttat taatctgcat actaatctgg
    33421 tatatattta tctcatcata atatacaatt attacccaca aaaatttaat gataaagata
    33481 tacaatctag ctagataaaa aaatgaaaca aacatgatct atttcacttt ttattatttt
    33541 aaaaaaataa ttataatatt tggttgttta aaaaatattt tataattttg caaaagtaaa
    33601 ttggtccggt agttcagttt ggttagaatg catgcctgtc acgcatgagg tcgcgggttc
    33661 gagtcccgtc cggaccgttt taatgtttat tgtgctttct taaaaaagga ttaaaataaa
    33721 ttttttttaa tcctttttta tttttatata tcatctagat tacaataaat atagagagtc
    33781 aaaaaacact agctatttga gtataaaaaa ttttatttat tataattttt gtatatacac
    33841 taaaaaatat ctaatgtttt cataatttta tacctgtact atttttttac aattacaggt
    33901 aatacgatta catcatcatc aaattttatc actaaatgat aaaccccatt actaatatta
    33961 tctagattta attgataagt atcagtattg gctaccattc tacccaaagc atctatcaaa
    34021 cgaacttcta aaattttctt atcagtctgt atgtatataa tatcctgtgc tggattagga
    34081 taaataatta cctcatcgtt ataatatgac tggatatcaa cattattaac tattatagca
    34141 ttagaaggta gagattgaca atcattttgt gtaactctga cataataagt gccatttact
    34201 atcacttgat aggtttgatt gatagcacct ggaatagctc cactttcatt ataccattga
    34261 ttaccagtag atacactaga aaataaaatt attggataag aacttatcat ggtaatagta
    34321 ggtgttggag gaatagaatt tactataata ttaatttgat tgcttgtggc attaccgcaa
    34381 tcatttgaaa ctaatactct gaaataatat gttcctacat tatttcctat aaaattataa
    34441 gaagatgtat tagcaccaga aatattaaac caagtattat tatcagtaga aatttgccat
    34501 tgtagggtag tcataggagt ttgtccagat aagataagta taacattctc attctggcaa
    34561 atattattgt catttacatt aatattacct gctacaggaa tagcattaac atttaaaata
    34621 atagtattac tagtagcatt accacaatta tttgttacca ttgctctaat ataaaaagtt
    34681 ccaacattat taggagtata attgtatgta aatgtagtag cgccattaat attactccat
    34741 gatacattat cattggatat ctgccattga atagtagtat ttggactata tccagataaa
    34801 gtgagtaaaa cgggattatt ttggcaaact gttgatgtat taattgatgc aatacctgaa
    34861 atgggtaatg aattgatcgt aatgataatc tcatcagaat aaacactacc acaactatta
    34921 gacactttag ctctgaaatg gaaaatgcca gagtttatag gtaagtaatt ataactacta
    34981 gtagtagctc ctattatatc tgaccaactt ataccatcag aagaaatctg ccattgaata
    35041 ttacttccta cagtatatcc tattaaatta agagataatg cttcattttg acaaatctgg
    35101 gtagcattaa tatttatatt acctgcacca ggcatagatt ctactattac agtggctgta
    35161 gctgtattag aacagttatt agaattagta cctgtaactg tgtatgtagt agtactagat
    35221 ggagatacag taataataga accactcata ttattactcc atatatatga tgatgcacca
    35281 ctcgctgtaa gtgtagagct actaccagca caaatatgtg atggattaga tgtaatatta
    35341 atagtaggta attgattcac agtaatagta acagtattaa tattatggca accagctgag
    35401 cttgttccag taacagtata aatagtggtt gaagttggtg atactgttat tgatgaacca
    35461 ctcataccat tgctccattg atatgtacta gcaccactag ctgaaatagt agtactttga
    35521 cctacacaaa tagtattagg aacagcagat acagagatat ctggtaaagg ataaacagtt
    35581 atagacacag tagcactacc tgtacatcca ccactactac ctgtaacact ataaatagta
    35641 ctggtcgttg gtgaaactgt aatttgagaa ccattcatac cattattcca agtgtaaaca
    35701 tctccacctg tagccataag tattgcatta tcacctgcac atatattcaa aggtgaagct
    35761 gtagctgaaa tatttattga tgtagtaata tttactgtaa ctgtcgcagt gtttgtacaa
    35821 gctgatgtat tcgtaccagt tacagtatat gtagttgttg tcgatggaga aacagttatt
    35881 gtagctgaat tcatgccatt attccagcta taactattag ctccagtagc tgttaataca
    35941 gaattaccac caatacaaat agagcttgga gaagcagtag ctgatatatt cggttttgca
    36001 tatacattta cactgatact tcctgtattc tgacatccag cagtactagt accggtgacc
    36061 gaatatgtag tgctagtaag aggtgatact gttattgatg aactactcat accattactc
    36121 cattgatatg tactagctcc actagctgaa atggtagtac tttgatctac acaaatagat
    36181 ggtggcacag cagttatagt gatattaggt aaaggattta ttacaattgt ttgagtgtca
    36241 ctatccgata ataagccata atgtgccgtt agtacaacat taaatgtgcc agcagtatta
    36301 taggtaacta ttggattagt ttgtgtactt gtagatggat ttccacctgg
//
LOCUS       DAOPRN010000014        34225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_994_length_34225_cov_132.996225, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000014 DAOPRN010000000
VERSION     DAOPRN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 34225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34225
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_34225_cov_132.996225"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..1085)
                     /gene="mtgA"
                     /locus_tag="PKI65_02780"
     CDS             complement(384..1085)
                     /gene="mtgA"
                     /locus_tag="PKI65_02780"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOB77400.1"
                     /translation="MKKGVKKFWKILYKIILIFLCITVGLVILYRWVNPPITPLMVIR
                     CVEQKKEGKTIKLEKKWVSIEEISPYMVQAVVAAEDNKFTKHFGFDFDAIKYAIEYNK
                     THKRKIGGSTITQQVAKNVFLFPHRTWIRKAFEAYFTVLIEIFWSKERIMEVYLNVVE
                     LGDGIYGVEKAAQIYFGKPAKKLNRIEAATLAASLPNPRKWNPHTQHPRLYRRRDKIL
                     SLMNKIGPVELKRSR"
     gene            complement(1164..2306)
                     /locus_tag="PKI65_02785"
     CDS             complement(1164..2306)
                     /locus_tag="PKI65_02785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOB77401.1"
                     /translation="MMQLTWDIPTRLHFGKDCIQKLGEIISSYASNVLIVTGANSSKK
                     NGSLNDVIDQLKRYNIKYHILDKIKSNPRVEEVREGILRAKKENVDAVLAIGGGSAID
                     SAKAIAMGYYYDGDVWDFYENILKPFKALPIVAVNTLAATGSEMNGTSVLQNESRQLK
                     ISVRSTILFPKDSFCDPTYTISVPQDYTIYGIADIIAHLWEAFFGMGEATLSDRFGKA
                     IFDEVMEFGPQLIDDLINYDLREKIMYASTCALNGITIMGKKTGDWGVHAIGHHLSLL
                     YDVPHGASLSVVYPIWLKWLQNKSAYGITKFGLTYFDTEAPDETIDKAIESFRSINAP
                     IKLEDLNIKNFDKNYFLQLLINNKASGAIYPLNESDLKWIVDEMVK"
     gene            complement(2403..2570)
                     /locus_tag="PKI65_02790"
     CDS             complement(2403..2570)
                     /locus_tag="PKI65_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOB77402.1"
                     /translation="MAKKTVAGYRDIKSKNVTKVIRMVKSPKTGSYQFKEYIMDSDKV
                     NDFLSKNSNNK"
     gene            complement(2583..2771)
                     /gene="rpmG"
                     /locus_tag="PKI65_02795"
     CDS             complement(2583..2771)
                     /gene="rpmG"
                     /locus_tag="PKI65_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOB77403.1"
                     /translation="MARKSKDARVQVILECTEQKNTGVPGISRYVTTKNRKNTPERLE
                     LRKYNPYLKKYTIHKEIK"
     gene            3103..3609
                     /locus_tag="PKI65_02800"
     CDS             3103..3609
                     /locus_tag="PKI65_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOB77404.1"
                     /translation="MVIHLMGSHSKYSKRYPMKYNKFNLAKNNKEKIINEYDNSILYN
                     DFVVNSLLYIKKQYCIENHEVIATAIYFSDHGENVYDENDNVGHDYADVLPKANVEIP
                     FIVWYSARYQSLYPNKLNIIYSNKNKPFITDNLFHAIIDINDITFKLFEPERSIFNKK
                     YNSNRKRI"
     gene            complement(3678..5042)
                     /locus_tag="PKI65_02805"
     CDS             complement(3678..5042)
                     /locus_tag="PKI65_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOB77405.1"
                     /translation="MKLKENKWLLISIIIGSFFNSFMSSSLNIAMPVIDIEYGIGVDK
                     VAWITKSFLLASAIGLLLSGWLADNYGRKKVFLIGNIAFGFISLIIIFLKNFYFIVIF
                     RFLQGLGSSMILASAPALIASAFESNKRGFVLGIQTTFTYIGLLLAPLLGGILTQYFG
                     WKSLFLFNFIFTLIALIVIFFGVKSEWKNEEKGKFDLYGYSLFVIVIIGIVLALEWIN
                     WNSLFALAISFFVGYGFIIYELQHPQPFISIPFIRSNKYFTNSILAALINYATTFAIS
                     YVLSIYLQIVRGLNPLKAGIILSFQPLMMALFSAYFGHLSDKHNPGKIASSGMAIISA
                     SLLLIGLLINESLPLYILAILLAILGFGFAVFTSPNTNAVMSSINKNAYGFASAFLAF
                     SRLFGQFISMALASSIILLINTKNIVIFKFHSLLLSTKIIFIIFSLLSAYGVYLSAQR
                     VKKT"
     gene            complement(5300..6304)
                     /locus_tag="PKI65_02810"
     CDS             complement(5300..6304)
                     /locus_tag="PKI65_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB77406.1"
                     /translation="MKKLILYSGCLLILLTSCYRDPSKNKEIELSNWEKKLKYIDSLL
                     AKNPKNDSLWAERALIFLSVDQIDSAYSSISKALLINSNVSRYHVILSDLYLARNSIN
                     NCREALLKAIDLDDENIDALSKIAELYFLLGDYEKSFAYLNKITNLDPNNAKAYFMKG
                     YGLLEQGDTTRAITAMHRATQADPDYYEAFLKLGQLLSSKDPPLADEYFNAAIRIKPN
                     SEEALYMYAYHLQSTNRINEAIQQYEKLIILNPVNKYAYYNLGYIYLVYKNDYNKSIY
                     NFTKSIEIDSSYAEAYYNRGLAYEQLGQVDKAIADYKTSLRYKTNFDKAIERLNKLKS
                     "
     gene            complement(6319..7320)
                     /gene="recA"
                     /locus_tag="PKI65_02815"
     CDS             complement(6319..7320)
                     /gene="recA"
                     /locus_tag="PKI65_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOB77407.1"
                     /translation="MATEDNEKLKALKLTIDKLDKTYGKGSVMKLGDNTVEPIETISS
                     GSIGLDLALGVGGYPRGRIVEIFGPESSGKTTLAIHALAETQKAGGIAAFIDAEHAFD
                     ISYAQKLGVDIENLLVSQPDHGEQALEIADALIRSGAVDIIVIDSVAALTPRSEIEGE
                     MGDSQMGLQARLMSQALRKLTANIGKTKCLCIFINQLREKIGIIFGNPETTTGGNALK
                     FYSTVRVDIRRVSQIKEGDKYLGNRVRTKVVKNKVAPPFKQAEFDIIFGEGISKIGEI
                     IDLGAELDIIQKSGSWYSYNNNKLGQGREQVKKLFKDNPDLADEIEKKIREKLTQNL"
     gene            complement(7327..7788)
                     /gene="bcp"
                     /locus_tag="PKI65_02820"
     CDS             complement(7327..7788)
                     /gene="bcp"
                     /locus_tag="PKI65_02820"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOB77408.1"
                     /translation="MLEKGQIAPDFSFKHVSGKEIKLSDLKGKKIILYFYPKDNTSGC
                     TKEACNFRDNYEYLTKLGFEVIGVSPDDETSHNKFREKYNLNFTLVPDPEKKIINLYG
                     VWGNKVRCGKESEGLLRTTFIIDENGIIQQVIKNVDTANSTQQILDILKIK"
     gene            complement(7972..8319)
                     /gene="rplT"
                     /locus_tag="PKI65_02825"
     CDS             complement(7972..8319)
                     /gene="rplT"
                     /locus_tag="PKI65_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOB77409.1"
                     /translation="MSRSVNHVASKARKKKILRLTKGYRGRNKNVWTVAKNRWEKGQT
                     HAYVGRKLKKRDYRKLWIQRINAAARLNGMSYSQFIGLIHKNNVALNRKVLADLAMNE
                     PEVFAKIVDSVKN"
     gene            complement(8424..8618)
                     /gene="rpmI"
                     /locus_tag="PKI65_02830"
     CDS             complement(8424..8618)
                     /gene="rpmI"
                     /locus_tag="PKI65_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOB77410.1"
                     /translation="MPKMKSKSGAKKRFVITGTGKVKRKHAYKSHILTKKSKKRKRNL
                     GHYAIVEGKDAKNVKKMLLK"
     gene            complement(8633..9166)
                     /gene="infC"
                     /locus_tag="PKI65_02835"
     CDS             complement(8633..9166)
                     /gene="infC"
                     /locus_tag="PKI65_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOB77411.1"
                     /translation="MAKQAEKFNYRINDEITAREVRVVGDGIESQILPIDKALEMAIE
                     KNLDLVEISPQANPPVCRIMDYNKFLFERRKKEKEIKAKTFQVVVKVIRLGFNIDEHD
                     FNFKLKHAQRFLEEGNKVKVEIIFKGRTIIYKEQGELQLLKFADALSEIAKVEQLPKL
                     QGKIMSMILAPKISKKK"
     gene            complement(9175..11124)
                     /gene="thrS"
                     /locus_tag="PKI65_02840"
     CDS             complement(9175..11124)
                     /gene="thrS"
                     /locus_tag="PKI65_02840"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOB77412.1"
                     /translation="MKDIVITFSDNSKKIYPAGITGMDIAQSISQSLAREALAIKVND
                     TLLDLERPIESDAKIQILTWEDREGKEVFWHSSAHIMAAAVFSLFPHVKFGIGPSIEQ
                     GFYYDIDFGDYKITEDDIEKIENKAKDIIKQDLPFIRKEITKAEAINLFEKSNDTYKL
                     ELIQELENEQITLYCLGEYIDLCRGPHLPSTKYVKTFKLLNIAGAYWRGDEHRQMLTR
                     IYGISFPSKKLLDDYLTFLEEAKKRDHRKLGKELELFMFNQKVGQGLPMWLPKGTIVR
                     EELEKFLKKIQIQYGYQHVITPHIGQKELYMTSGHYEKYAKDSFRPISTPNENEEFML
                     KPMNCPHHCEIYAFKPRSYKDLPLRIAEFGTVYRYEQSGELHGLTRVRSFTQDDAHIF
                     CTNDQLNEEFHKVMEIVLLILNTFDFKNFTAQVSLRDPDNKEKYIGTDENWDKAEEAI
                     LESVANTEFNIIIAKGEAAFYGPKLDFMVQDAIGRSWQLGTIQVDYNLPERFNLEYIG
                     PDNQKYRPVMIHRAIFGSMERFVSLLLEQTAGKLPLWLAPVQVIVLPISEKYIEYGKK
                     VVSLLSNFDVRTEMDDRSEKIGKKIRDAELEHIPYMLIVGEKEQNDNALSVRRQGIGD
                     IGVMPIESFMNIFSEEINKSLTNKN"
     gene            11630..12454
                     /locus_tag="PKI65_02845"
     CDS             11630..12454
                     /locus_tag="PKI65_02845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOB77413.1"
                     /translation="MLSIVILNYNTFDFTCQCIKSIYDYTKDVDFEIIVVDNASTTDQ
                     ADSFLELFPYIKLVKNYKNKGFAGGCNDGIKVAEGDTILLINSDTKLLNDAISITYNF
                     LKKHPEVGIVTCRLENEDGSPQNNCQIFPRISKILFEIFRINKMLPIKKTGKILYGPF
                     FSYTEIAYPDWVWGTFFMFDKKLLDIFPNKLLPETFWMYIEDMQWCWLASQKGYEIVF
                     VPEARVLHLGGKNHSERARAMMDKNLSQFLYLYYNKIHAKLIIYLSKLLELSQWRN"
     gene            12790..13800
                     /locus_tag="PKI65_02850"
     CDS             12790..13800
                     /locus_tag="PKI65_02850"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77414.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEIVTSNRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNITYVNLN
                     IC"
     gene            14172..15158
                     /locus_tag="PKI65_02855"
     CDS             14172..15158
                     /locus_tag="PKI65_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOB77415.1"
                     /translation="MHKLYPFKFKPQFFQKVWGGKNLTKLYHLENDDTLGEAWLLSSI
                     EGKESEIINGEWAGNNIVEMIEVFMDDILGEHIFYTYGDTFPLLIKLIDTSEWLSIQV
                     HPDDNWAKKLEKQQTCGKNELWYFIKTANNAQILHGLSRLADQDIIMKYLNEKNIEGI
                     FRNINVSPHSYIYTPAGTVHALGPGLFLAEIQQTSDITYRIYDWDRPQDPKNPRPLHL
                     DKAFKVINYDAIPEDLQYQPIIADRTVNLFNGENFVINRIVLSSGKQIEKDIEPFDSF
                     FVYLILNGNGKIICDTYVVEIMAGDCCLIPACFSNVIIQSDKDILALEVYIQ"
     gene            15298..15396
                     /gene="ffs"
                     /locus_tag="PKI65_02860"
     ncRNA           15298..15396
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKI65_02860"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            15424..16044
                     /locus_tag="PKI65_02865"
     CDS             15424..16044
                     /locus_tag="PKI65_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOB77416.1"
                     /translation="MKFFLNTVYIEEVRKIKELQIIDGINTNLAGMEALGVKDINQIL
                     THYNVICQTFNKTVLVDVKAKDYSSILREVDILTSVNELITVKIPANLDGIKAINTLA
                     NQKFDIVCSSIFSTSQAILAAEAGALYIFIPMKKAMQFGIDTLHLLTQIINIYNEQGY
                     DTYIIADGISSSIELTQMALLGADGISCDIQTIYSFFQNNFSNNKM"
     gene            16099..16746
                     /locus_tag="PKI65_02870"
     CDS             16099..16746
                     /locus_tag="PKI65_02870"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOB77417.1"
                     /translation="MLLKIHPENPQQRLIVKVVDILNDGGIVIIPTDTVYAFATNPFK
                     KSGVEKLCMLKKLDPSKATFSFLGSNLSILSEYTKPIPNEIFRLMKSVLPGPYTFILP
                     INSKIPKILENRRKTIGIRIPDNKIVQAIVDTIGHPIVSSSIRVEEQLGNEYISDPEL
                     IYERYLEFVDAVVDGGPGSLVPSTVIDATEDMPILIRKGLGPVDFLDLIDEGENI"
     gene            16727..17053
                     /locus_tag="PKI65_02875"
     CDS             16727..17053
                     /locus_tag="PKI65_02875"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOB77418.1"
                     /translation="MKEKIFNFFNFNVKRKSEEIFENIFTNENIRIQRILSFGQVSPP
                     DYWYDQDEDEWVLLLKGKAKLEFENEIITLHRGDSLLIPAHMKHRVAYTSKYFTTIWL
                     AVFIKI"
     gene            17123..17392
                     /locus_tag="PKI65_02880"
     CDS             17123..17392
                     /locus_tag="PKI65_02880"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOB77419.1"
                     /translation="MSMITINKNITEDKLEKIKKWPIWEKDISRFPYYYDHTEECYII
                     EGRITIETEDGIYHIEPGDFVRFEKGLSCIWDIKEKVRKYYDFPK"
     gene            17389..18447
                     /locus_tag="PKI65_02885"
     CDS             17389..18447
                     /locus_tag="PKI65_02885"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOB77420.1"
                     /translation="MKFKKGDKVKYLDEIGEGIVKEIHGDLVIVEDDLGFEIPMKANK
                     LILVEHSKNMNNIDDTTDMQDNIIEGDTKTDLSILSNHNKSKIQQFTDGIYLVIKPEN
                     PKVPIASPHQLYLVNFTSYDVYFLLHQIKVGNTNIYHSFLNDYTAIKLENLKIEDMPN
                     ELLCTLQSIIISKEHIFLPFISTFAIKRSTLLKEDNYSENPFFDEPTISILIKDLKEI
                     NELKNFEKQKDELEINFSKSKERLIVDASIIDKYLVSPELAEVDLHLEKLVDDPQNIS
                     PDMALNIQLSYFKQCLDAAISKDIDKIIFIHGVGAGILKREMYQILKNYPDLYYGDAP
                     IRQYGIGATEVYIKPIKK"
     gene            18453..19124
                     /locus_tag="PKI65_02890"
     CDS             18453..19124
                     /locus_tag="PKI65_02890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOB77421.1"
                     /translation="MAKIPIESILFIDVETVPIAPDYQTLDDNLKSHWDKKASFLTKN
                     DEDDPSSVFERAGIYAEFGKIVCISAGRVQNDEIKTKSYYSHNEKQLLEEFTNDLNIF
                     TRNIPNLYLCAHNGKEFDFPFIIRRMIINQIPVPYILNTRNKSRYELPFLDTMEMWKF
                     GDYKHFTSLALLCELFGIPTPKNDIDGSQVASVYYKENNLERIKEYCEKDVCALIEVY
                     KRLDF"
     gene            19184..20179
                     /gene="aroF"
                     /locus_tag="PKI65_02895"
     CDS             19184..20179
                     /gene="aroF"
                     /locus_tag="PKI65_02895"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOB77422.1"
                     /translation="MILHVKKDIDKNLLNNVVNYYNGVLLEKDEYFIIISSKNSNIKN
                     EWQQYIIEKFELNSECQLSSREYKKETKRIDLPCGYIGGDEKNTIIIAGPCAVESEQQ
                     IFESAKFIKSLGINAFRAGLYKSRSSPYTFQGLGDKGLNLLYKIKNEFNLIIVSEVRD
                     ITNFDKIANLADILQVGAKAMYDQAILRACGESSKPVLLKRHFGANISDFIQATEFIL
                     SAGNEKVILCERGIRTFETKTRFTLDLTGVAYMKKYLNLPVVVDPSHAMGYSYGVPAL
                     TKACMAMGIDGLLIEVHPSPQKALSDAKQQLNHKEFTDLMVSLKRIAKAIDKNII"
     gene            20196..20861
                     /locus_tag="PKI65_02900"
     CDS             20196..20861
                     /locus_tag="PKI65_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOB77423.1"
                     /translation="MLDILKKNIEYFLEQLPDLQDLTKGKEAYSLSDIRALADLFNVP
                     FNYCFKYDLRKEDILIKDFSNRIKVLALDVDGVLTDAGMYIASNENEIKKFNAQDGIA
                     IQYAINKGIIVILLSSGLRDEIISYRARMLKIPLFYVGRESKWDKLNNFCNDNNVTPE
                     DVAYIGDDINDLFVLENVGLSICPQNAVAKVHKAVKIHLFKNGGEGAVRELVERLYQD
                     LFD"
     gene            complement(20862..23288)
                     /locus_tag="PKI65_02905"
     CDS             complement(20862..23288)
                     /locus_tag="PKI65_02905"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOB77424.1"
                     /translation="MKEYIFYILFFIFSISAFTQERQITAYRTNEEIKIDGLINEEAW
                     SKAECTGDFVTYKPYQGQMPYKPTKVCVLYDDRALYIGAILYDDEPDKILKELTMRDQ
                     DNGNTDMFILQLNPYNDKRNVYEFKVTAANVQTDIMISDGNYDYNWDAVWQSGVTIHE
                     NKWTVEIAIPYSAIRFPNIPEQSWSANFWRVIRRNREYSCWNPVKQELGPDIEQMGKI
                     IGIRDIKPPIRLAFYPYISTNIDFYTSAKKPVYGLSGGLDMKLGITKSHTLDLTLVPD
                     FSHIKSDEEELNLGPFETYYSEYRPFFTEGVELFEKVGLFYSRRIGKIPAKYDEIRSM
                     KDDNYVIIDNPRYARLINAIKLSGRNEKKLGIGVFNAITANTWAEVKDSAGNIHKILT
                     EPWANYNMVVLDQSFWHNSYVNLTNAFVIRPDINYLSNVIGTAFKFMDPSNRFGLYGK
                     AAWSCINDSIGNNDLDNGFMINSGLGKLNGKWQYVYELNLLSDRYNPNDFGYLQQNNI
                     ITHHLSLSYNIFQPFGKFNKMSIYTDLDHHRLFKSNDLVQTKLLLNAYLETNKFFSTW
                     IYLDFSLSDHYDYFEARAPGRLYRRPAIQSTYIYLSTDYRKVFALDFRIGAYLDFIKR
                     NGWWGLIKPRVRISDKITLIYGLDFDFDHNDCGFVNDTIYNDEIIFGLRNVNTITNSL
                     EGRWVLNNKMYFNLTIRHYWRTVDYSEFYFLNQDGSLSIINNYAQDHDINFNAFNVDF
                     MFSWNFAPGSYMNIMWKNQIFSNEIIHYYTKMPSFNENLSNLFKNDQTNSISLRLIYY
                     FDWQYLKKNT"
     gene            complement(23419..25377)
                     /locus_tag="PKI65_02910"
     CDS             complement(23419..25377)
                     /locus_tag="PKI65_02910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB77425.1"
                     /translation="MTLSIIIVNYNVKHFLEQCLLSVFDALESIDGEVIVVDNSSVDG
                     SVAMIKQKFPQVTLIANNENLGFSKANNQALNIAKGKYVLLLNPDTIVERDTFTKTIA
                     FMEQHPDAGALGVKLVNGKGQYLPESKRGIPTPWVSFCKMSGLIKLFPHSKTFAKYYM
                     GHLDPDEINEIEILSGAFMLIRKEVLDEIGYLDETFFMYGEDIDLSYRILQAGYKNYY
                     YPHTRIIHYKGESTKKGSINYIYNFYNAMLIFTQKHLYTKGAKWMKFFISIAIYSKAF
                     IAFLKRLFKKIWLPFLDLLILYSGLYGITILWEKIRFSYDSIIYPRPFLYYALLIYSL
                     VWIIAIFLNGGYDKPFHKKHFFTGIISGSIILLLAYGLLNEQYRFSRAILLLGTLWAL
                     LSLIAIRYLIEWLHLDSWGLLKQNKRIAICGDEQDIKAVKNVLEQSNVSVDQLFYIDP
                     EDISDSNFYHANINQLIDIIRIYKLNEIVYCTNSVAMSKIIDSMSSLSDHHVDFRISS
                     PTNEFLLSSRYILNPEDVFLYELNSIGKPVNRRRKRVFDFFTSIILLIFFPFYMLFIK
                     KSGQAFKNIISVLLNKKTWVGYCTKNKFHNLPKIPEGVFTPLDGLGFNPQSDISNEID
                     FNYARDYHVMTDLSILLRNLNNLGKIND"
     gene            complement(25404..27086)
                     /locus_tag="PKI65_02915"
     CDS             complement(25404..27086)
                     /locus_tag="PKI65_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOB77426.1"
                     /translation="MLVNKNTKENRNENKNFIEEIIDRHLLEGRFETVKTRFPPEPNG
                     YLHIGHAKSICLNFGIAKKYNGTCNLRFDDTNPSKENKEYIDSIKEDVKWLGFQWDKE
                     CYASDYFEQLYEWAILLIKKGKAYVCDCPQELISKNRTKPTEPGIPCKHRDRSIDENL
                     LLFNKMKTGEYSAGSRVLRAKIDMNSPNMHMRDPIMYRIMYENHPRTGDKWCIYPTYD
                     WAHGQSDSIEKITHSLCTLEFEVHRPLYEWFIKELNIWAPQQIEFARLNLSYTIMSKR
                     KLLQLVEGGYVSGWDDPRMPTISGLRRRGFTPESIKMFIEIVGIAKRENLIEYELLEH
                     CLREDLNKRAPRRMAVINPLKLVITNYPENQVEWLEALNNPEDPMSDTRLVPFSKEIF
                     IEHSDFMENPTKKYHRFYVGNEVRLRWAYFIKCTGFKKDDNGNIVEIYGEYDPASRGG
                     NSPDGRKVKSTIHWVSAEHAIPIVVRLYEKLFTIPDPDNVEADKTFIDYLNPNSLITT
                     TAYGEPALNNYLTPAHYQFERLGYFVTDPDSNTEHIVFNLITKLKDSFAKKI"
     gene            27120..27512
                     /gene="folB"
                     /locus_tag="PKI65_02920"
     CDS             27120..27512
                     /gene="folB"
                     /locus_tag="PKI65_02920"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOB77427.1"
                     /translation="MTTVIYLKNLKFHAYIGCFYEEKLIGQLFDLNLRIELDLTKPMI
                     TDNINDTINYQNVYKIISELMKLKYNLIEHAAYHILDKLWQIYPNIQKIELEINKLNP
                     PIKGEIGSAAFFISIEKNEFEQLKNLQI"
     gene            complement(27599..30745)
                     /locus_tag="PKI65_02925"
     CDS             complement(27599..30745)
                     /locus_tag="PKI65_02925"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOB77428.1"
                     /translation="MLKQYLASIQNTLNRGDAREENYYGHLKDLLLAFVGQNNYNKVE
                     ITILPKQTEAGNPDFRVWDGKNHITGYIEAKDPSVTNLDKIENSEQLKRYISTFPNVI
                     LTNFYEFRLYRNGDRIKTTTIARYAIAKNLNVAPPVENTNDFYELLDTFFSFSLPKVN
                     NAKNLAVELANRTRFLRDEVVSIEMAEELKKGQKAIRRFYEAFQKYLIGTLTEKTFAD
                     LYSQTITYGLFAARTRANDEFNRELAFKYIPQTIGILRDIFRFISLEDAPKSLQIIVD
                     DIAEILQVTDVKKILQDFYLDGKGQDPIVHFYETFLSQYDPKIRKKRGVYYTPEPIVN
                     YIVRSINDILKTHFDIADGLAGKEVTLLDPAAGTLTFPAEAIKLAINEYTDKYGTGGK
                     ENFIKNHILENFYAFELMMAPYAIGHLKISFLFDELGYKMSNDERFKLYLTNTLEKEN
                     LDQTIIPGLSSLSEESHLACEVKKDKPILVILGNPPYSGISANINEWTEQLLKKDING
                     ALSYYKVDGMPLGEKKLWLQDDYVKFIRFAQCKIHKAGQGIVGMITNHSYLDNLTFRG
                     MRQSLMNTFNEIYLLDLHGNALKKEKCPDGSKDENVFDIRQGVVIAIFVKQKNKEDCR
                     VYHKDLYGMREAKYQWLLDNNFDKKNYTEIRPASPEYFFIPRNIEDIKYYEKWDKIND
                     IFPVNGVGIVTANDNLAIDFTKEALENKIRIFRNKNIDHETIRKTFKIKDNNQWNLSK
                     QRERFMQCDNWMSCIKKILYRPFDIRYIIYHDDIIARTRKNLMSHMLNENLGLITTRF
                     VFKKNDDFCHAFITNNIIDINQIQSPGTAQIFPLYLYTEQQNNTKTPSAHTMMLFERS
                     VEYHTRQPNIDKSIYEKLNKTYNKHLTPKEILFYVYAVLYSNIYRKKYADFLKIDFPR
                     IPFAKNYKLFNKMSTLGNKLVDLHLMKSKELDNVIAKYEGEVGTNKIEKVEYNEKKKR
                     IYINNDKYFTNVSPQLWNYHIGGYQVLYKFLKDRKNLNLDNPEYYCKIITAINKTMDI
                     QKQIDKLYPLIEKNLYNI"
     gene            30938..31876
                     /locus_tag="PKI65_02930"
     CDS             30938..31876
                     /locus_tag="PKI65_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77429.1"
                     /translation="MKKLFLIIISIFSFLVLQSQVNNQESKNYSEDIATLKKSMTIVQ
                     RQIKNQQLIISNQNNKIDSLNKIIAVLHNEIEEGSSNQATINESINDLSKRIVNINNA
                     LAERKTYVIIGSIIAIIILLLILYYLRKNFSKIKSTIKQKENELNERITEINDNFNNE
                     INNVKILIENNKNEFNEKIIKFQNDFRNNLETNVNNLQSLINEKYQLLTNEFNEKLSN
                     TIDNLTITINEIKDNSNNLKSSVNKEINTIHTMITNINTNLDEQKNNISELDSKLVAI
                     NSELDVKISNLQKELENNIIEINEKINNLLKPTQNK"
     gene            complement(31910..32704)
                     /locus_tag="PKI65_02935"
     CDS             complement(31910..32704)
                     /locus_tag="PKI65_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HOB77430.1"
                     /translation="MLEIILALIPALFFLLLVYFKDKYKPEPFKYLFFAFFLGMLATI
                     PASFLENMLFSNIKLNLPLELSAFIEAFFFVALIEEGIKFLFFRWFIWPKKYFDEPID
                     GVVYMVAIGMGFAFLENLAFIFNDLSHIWTVALMRAIMPTPAHFVFAVAMGYYFGYAR
                     FNLVKRKNKLMFIGFMITFGSHGFYDYLLMLVPKYPINLSIAILFVATYLIVAIHLIN
                     RHQKLSPFVKRYKWINEQKRKNKEELNEQLNELKNKLKEKYNDRDR"
     gene            complement(32896..33306)
                     /locus_tag="PKI65_02940"
     CDS             complement(32896..33306)
                     /locus_tag="PKI65_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOB77431.1"
                     /translation="MISTIELTLPSHKRGFHLITSIIVEAIKPLPEMGLLNIFLQHTS
                     AALTINENYDPDVRVDMETIINKLIPENLPEITHNIEGSDDMPAHFKSSIFGVSLSIP
                     IVNYQLALGTWQGIYLCEFRNHASSRKLILTVIS"
     gene            complement(33444..34028)
                     /locus_tag="PKI65_02945"
     CDS             complement(33444..34028)
                     /locus_tag="PKI65_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB77432.1"
                     /translation="MSDYNGCTATAKITVNDLPGPKANFAPRPQVTTINNPTIYFENF
                     SIGAISYYWDFGDGHTSTEFAPIHNFWDVGTYHVILYAEDQYGCIDTVGANVFIKDIT
                     TFYLPNAFTPNNDDLNEVFNFVGAEINPINFEMRIFDRYGKQLFYTTDINQGWDGTYK
                     GETVPEGVYTIAINFVSSITGEKHFIIDNLIIIK"
BASE COUNT        12092 a         5057 c         4767 g        12309 t
ORIGIN      
        1 taaacaatgt ggtgcccgat gtaggtagga tgctcaatct cgcatcatca gacaggcgag
       61 cgtggtatgt atacagagac ttgagagcaa gtcctgtgta tatttaaaca ctgaagaata
      121 atattactcc cccttccaaa acatttctta ttttatttta caatactttt tggttgatta
      181 tcttaccatt tatatttatt ttgataagtt atatttgtag taattttgat taattttttt
      241 attattaacg atataaaaaa agtataatgc ttattcttga ataattaaat taatataata
      301 ttatatttta gaattaaatt tagtacttac atctcttatt atcaaaaaat tataatatat
      361 acatttgttt tggggatttc ctttcatcta cttcttttca gctccactgg accgatttta
      421 ttcataagcg ataagatttt atctcttctt ctatataatc ttggatgttg agtatgagga
      481 ttccattttc gaggatttgg taatgatgct gctaatgtag ctgcttcgat tctatttaat
      541 ttttttgctg gtttaccgaa atatatctgt gctgcttttt ctacaccata aataccatct
      601 cccaattcta ctacatttag gtaaacttcc attatgcgtt cttttgacca gaaaatttct
      661 atcagaaccg taaaatatgc ttcaaaggct ttgcgaatcc atgtacgatg aggaaataga
      721 aaaacatttt ttgctacttg ttgtgttata gtgcttccac ctatttttct tttgtgcgtt
      781 ttattatact ctatcgcgta ttttatagca tcaaagtcaa aaccaaaatg tttagtaaat
      841 ttattatctt cggcagctac taccgcctgc accatataag gactgatttc ttcgatagat
      901 acccattttt tttcaagttt aattgtttta ccttcttttt tttgttctac acatctgatt
      961 accatcaaag gagtgatagg aggatttacc caacgataca aaattactaa tcctacagta
     1021 atacacagaa aaattaatat gatcttatat agtattttcc aaaatttttt cactcctttt
     1081 ttcattatag ctttgtaata gtgatttaat taagtgttaa tgttttttta ttatttatta
     1141 taaattattt aatactcata aattcatttt accatttcat ctactatcca tttaagatca
     1201 gactcattta atggataaat agcaccacta gctttattat taataagaag ttgcaagaaa
     1261 taatttttat caaaattttt aatattcaaa tcttctaatt taattggcgc atttatagat
     1321 ctaaaagatt caattgcttt gtctattgtt tcatcagggg cctcagtatc gaaataagta
     1381 agtccaaatt tagtaattcc ataagcactt ttattttgca gccatttcag ccaaataggg
     1441 tatacaactg aaagagaggc accgtgaggt acatcatata aaagagaaag atgatgacct
     1501 atagcatgta ctccccaatc acctgttttt ttgcccatta tagttattcc atttaatgca
     1561 caagtagatg catacataat tttttcgcga agatcataat ttataagatc atctatcaat
     1621 tgcggtccga attccattac ttcatcgaaa atggctttac caaatctgtc tgacaaagta
     1681 gcctctccca tgccaaaaaa ggcctcccac aaatgagcta taatatctgc tataccataa
     1741 atagtataat cttgcggtac agagatagtg taagtgggat cacaaaaaga atctttagga
     1801 aaaagtattg tagaacgtac agaaattttt aattgtctag attcattttg caataccgat
     1861 gtaccgttca tctcgctacc agtagcagct aaagtattaa cggctacaat tggcaaagct
     1921 ttaaaaggtt tcaaaatatt ttcataaaaa tcccatacat caccatcata ataataaccc
     1981 attgctatag ctttagcact atcaatagca cttccaccac ctattgctaa cactgcatcg
     2041 acattttctt tttttgctct taatattccc tctcttactt cttccactct tggattagat
     2101 tttatcttat ctaaaatgtg atatttgata ttataccttt ttaattgatc tattacatca
     2161 tttagactgc catttttttt ggaagaattt gctcctgtta ctatcaatac atttgaagca
     2221 tatgacgata ttatctcccc taatttttga atacaatctt tcccaaaatg tagtctagta
     2281 ggtatatccc atgttaattg catcataatt tttttttaat tttcttgcaa atatatataa
     2341 acaaaaaaag ataagcttct tataagaagc ttatcttttt tataagaata atattttaaa
     2401 ttttacttat tattactatt ttttgataaa aaatcgttta ctttatcact atccatgatg
     2461 tattctttaa attgatatga acctgtttta ggtgatttca ccatacgtat cactttggtt
     2521 acatttttac ttttaatatc gcggtaacct gctactgttt tctttgccat attattttaa
     2581 acttatttga tttctttgtg tatagtatat ttttttaaat atggattata tttccttaat
     2641 tctaatctct ctggagtatt tttgcgattt tttgtagtta catatcgaga tatgccaggt
     2701 actccagtat ttttctgctc tgtgcactct aaaattacct gcactcttgc atcttttgat
     2761 ttccttgcca tatttacctt aatatttatt ttagtttgca aagttaataa taatttttat
     2821 aacaaacaaa ataaattata aaaatattat cgtcaataca actactattc ccataaataa
     2881 aagataaaca atccaataaa aggcttcttt tttggtattc atgctacttt ttttattaga
     2941 caatataaaa atcaaaaagt ttattatttt aaattaaaat atttaaagat aaagtgtaaa
     3001 aaaaacacta taatatgctt aaattatcac ataaattaag caattattaa agttattatg
     3061 aactaattaa attatttgac ttggtaaaat aaaaataaat ttattgttat tcacttgatg
     3121 ggtagccatt ctaagtattc taaaagatat ccgatgaaat ataataaatt taaccttgct
     3181 aagaataata aagagaaaat aatcaatgaa tatgataatt ctattctata taatgatttt
     3241 gtagtaaata gtttgctata tattaaaaaa caatattgta tagaaaatca tgaagtgata
     3301 gcaacagcta tttatttttc agatcacggt gagaatgtct atgatgaaaa cgataatgta
     3361 gggcatgact atgcagatgt attaccaaaa gcgaatgtgg aaattccatt tattgtttgg
     3421 tactctgcca gatatcaatc attatatcct aataaattaa atattattta ttcaaataaa
     3481 aataaacctt tcataacaga taatttgttt catgcaatta tagatattaa tgatattact
     3541 ttcaaactat ttgaaccaga aagaagcatt tttaataaaa aatataattc taatagaaaa
     3601 cgaatataag aagataataa agattatgat ttgaaataaa tataggaagt tttaactaat
     3661 gttttaataa aataaaatca agttttcttt actctttgtg ctgatagata aactccatag
     3721 gcactgagca aagaaaagat aataaatata atttttgtag ataataataa agaatgaaat
     3781 ttaaaaataa caatattctt agtatttatt aataaaataa tactagaagc tagagccata
     3841 gaaataaatt gaccaaaaag tctactaaag gctaaaaaag ccgaagcaaa gccataagca
     3901 tttttattaa tagaactcat aacagcatta gtattgggtg atgtaaatac agcaaaacca
     3961 aagccaagaa ttgctaaaag tatagccaaa atatataatg gcaaggattc gttaattaat
     4021 aaaccaatta gtagaagtga tgcagaaatg atagccattc cactagaagc tatcttccca
     4081 ggattatgtt tatctgataa atgtccaaaa tatgcagaaa aaagcgccat catcaaaggc
     4141 tgaaaactta agattatgcc agctttcaat ggatttaaac ctctaacaat ttgtaaatat
     4201 atgctcaaaa cataactaat agcaaaagta gtagcatagt taatcaaggc agctagaata
     4261 gaattagtaa aatatttatt acttctaata aatggtatag aaataaatgg ttgtggatgc
     4321 tgaagttcgt aaattataaa tccatatcct acaaaaaaac ttattgctaa tgcaaataat
     4381 gaattccaat taatccactc aagggctaaa actattccta taattactat tacaaataag
     4441 ctataaccat agagatcaaa ctttcctttc tcttcatttt tccattcact ttttactcca
     4501 aaaaatataa caattaatgc tataagtgta aaaataaaat taaataaaaa caagcttttc
     4561 caaccaaaat attgggttaa tattccacct aataatggtg ctaataaaag gcctatatat
     4621 gtgaaagtag tctgaatacc taatacaaat ccacgtttat ttgattcaaa ggccgaagct
     4681 atcaatgctg gtgctgaagc taagatcata gaacttccca aaccttgtaa aaatctgaat
     4741 attactataa aataaaaatt ttttagaaaa attattatta aagatataaa accaaaagct
     4801 atatttccta ttaaaaatac ttttttgcgt ccataattat ctgctaacca tccagataat
     4861 aataatccta tcgctgatgc taataaaaat gattttgtaa tccaagctac tttatccact
     4921 cctataccat attctatgtc tataacgggc atagctatgt ttaaagaaga agacataaaa
     4981 ctattaaaaa atgaacctat aataatagat atcaataacc atttgttttc ttttaatttc
     5041 attactatat cagatttttt taaagaaaat aataagcaaa attatttaaa atatttgatt
     5101 gtacttagtg tacaaaaatc taaaatgaat tactcaaaag taaaaaatta aaaatgaacc
     5161 actataatta aattacaaat aacttaaata tttatttgat ttttttggtt tgttgtagat
     5221 aatgttatct atgagtatta tatttgttta gaatttagaa ttcgaatgtt ataaattatg
     5281 gtttttgaag tactgtattt caagatttca acttattcaa tctttcaata gctttatcaa
     5341 aattagtttt atatctaagg ctggttttat aatctgcaat tgctttatct acttgaccta
     5401 attgttcata ggctaatccg cgattatagt atgcctcggc gtagctggag tctatctcta
     5461 tcgattttgt aaagttataa attgatttat tataatcatt tttatagact agataaatat
     5521 agcccagatt gtaatatgca tatttattta ctggatttaa tataattaat ttttcgtatt
     5581 gctgaatagc ttcatttata cgattagtag actggaggtg atatgcatac atataaagag
     5641 cttcttcaga attaggtttt attcttatag ctgcattgaa atactcatct gctagaggag
     5701 gatctttgct actaagtagt tgtcctaatt tcagaaatgc ctcatagtag tcaggatctg
     5761 cctgagtagc acgatgcata gcggttatgg ctctagtggt atcaccttgt tctaataaac
     5821 catagccctt cataaaatat gctttagcgt tgttggggtc taaatttgtg attttattta
     5881 aataagcaaa acttttttca taatcaccta ataaaaagta aagttcggca attttagata
     5941 gggcgtctat attctcatca tccagatcaa tagcttttag aagagcttcc ctgcaattgt
     6001 ttatagaatt tctagctaaa tatagatcac tcaatatcac atgatatcta gaaacattag
     6061 aatttattaa taatgcttta ctaatagaag aataagcact atctatttga tctacagata
     6121 aaaatattaa agctctttca gcccacaaag aatcattttt tggattttta gccaaaagtg
     6181 aatctatata ttttaatttt ttttcccagt tagataattc aatttcttta tttttagatg
     6241 gatctctata acatgaagta agtaaaatta ataaacagcc actataaaga attaattttt
     6301 tcataatgag aatgtcaatt ataaattttg ggttaatttt tctcttattt tcttttctat
     6361 ttcatcggct aagtcaggat tatctttaaa taattttttc acttgttctc taccttgtcc
     6421 tagtttatta ttattataag aataccaaga tccgcttttt tgtattatat ctaattcagc
     6481 tcctagatct attatttcgc cgattttact aatgccttcg ccgaaaataa tatcaaattc
     6541 agcctgttta aaaggtggag ccactttatt tttaactact ttagtacgca cgcgattacc
     6601 taaatattta tcaccctctt ttatctgact cacacgtcgt atatctacac gtacggtaga
     6661 ataaaatttc aaagcattac cacctgtagt agtttctgga ttaccaaata ttatgccgat
     6721 tttttcgcgt aactgattaa taaagataca tagacattta gttttgccta tattagcagt
     6781 gagtttacga agcgcttgag acatcagacg tgcttgcaaa cccatctgag agtcccccat
     6841 ttcaccttct atttcgctgc gtggtgtcag agctgctact gagtctatca ctattatatc
     6901 aactgcacca ctacgtatga gagcatctgc tatctctaga gcttgctctc catgatcagg
     6961 ttgtgatact aataaatttt caatatctac accaagtttt tgtgcataag atatatcaaa
     7021 tgcgtgctcc gcatctatga aagctgctat gccacctgcc ttttgtgttt ctgctaaagc
     7081 atgtatagct aaagtagttt ttccagagct ctcaggacca aaaatttcta ctattctacc
     7141 cctaggatag ccacctactc ctaaagctaa atccaatccg atactgcctg atgagatagt
     7201 ctctataggc tctacagtat tatctcctaa cttcatcact gatcctttgc cataagtttt
     7261 atccaattta tctattgtta gctttagtgc ttttaatttt tcattatctt ctgttgccat
     7321 aataaattat ttaattttta aaatatctaa tatttgttga gtgctattag ctgtgtcaac
     7381 atttttgatt acttgctgta taataccatt ttcatctata ataaatgtag ttcgtagcaa
     7441 accttctgat tctttaccac atctaacttt attgccccaa acaccatata gatttattat
     7501 ttttttttct ggatcaggta caagagtaaa atttaaatta tatttttctc taaatttatt
     7561 atgagaagtt tcgtcatctg gactgacacc tataacctca aagccaagtt tagttaaata
     7621 ttcataatta tctctaaaat tgcatgcttc tttagtacat ccactggtat tatccttagg
     7681 ataaaagtat aaaattattt ttttaccctt gagatcagat aattttatct ctttgccact
     7741 aacatgttta aaactaaaat caggtgctat ttgacctttt tctaacataa tttttttgta
     7801 aaattaatca aaacatttat aaaaataaaa ttcttaaatt aaattaatta aaaaatatat
     7861 tctatttgta tcaaatatat tttttaatta atttaatttt taataaatat tattataatc
     7921 tgcaattaat aaattttatt acaagatttt aataattaag atttaaagaa attaattttt
     7981 aacgctatct acgattttag caaaaacttc aggttcattc attgcgagat cagctaatac
     8041 ttttctatta agagccacat tgtttttgtg aattagtcct ataaattgag aataagacat
     8101 gccattcaac ctagctgctg cattgatacg ttggatccac aattttctgt agtctctttt
     8161 ttttaattta cgacccacat acgcatgtgt ttgtcctttt tcccatcgat ttttagctac
     8221 agtccacaca tttttattac gcccacgata tccttttgta aggcgtagaa ttttcttttt
     8281 tctagctttt gatgctacat gattaacact tcttgacata atcaacctcc ttcttgtttc
     8341 agcggctttt tactattcta atttttatca aataattaaa aatgctctta ggggctgaaa
     8401 acaatttttt aaaaaacaat tttttatttc aataacattt ttttaacatt tttagcatct
     8461 ttgccttcta caatggcata atgcccaaga tttcttttac gttttttact tttttttgtc
     8521 aaaatatgac ttttgtaagc atgtttacgt ttcactttac cagtaccagt gattacgaaa
     8581 cgtttttttg caccagattt acttttcatt ttaggcatat ctaattaatt ttttattttt
     8641 tcttacttat tttaggagct aatatcatag acattatttt accttgcagt ttaggtaatt
     8701 gctctacttt ggctatttca gatagagcat cagcaaactt aagtaattgt aattcgcctt
     8761 gttctttata tatgatagtt cgacccttaa aaattatttc tacttttact ttattacctt
     8821 cttcgagaaa tctttgagcg tgttttagtt taaaattaaa atcgtgctca tcaatattaa
     8881 agcctagtct aataacttta accactactt gaaaagtttt tgctttaatt tctttctctt
     8941 ttttcctacg ttcaaataga aatttattat agtccattat gcgacagaca ggtgggtttg
     9001 cctgtggcga aatttctact aagtccaagt ttttttcaat agccatctct aatgctttat
     9061 ctataggtaa aatctgtgat tctattccat cacccactac tcttacttct cttgcagtga
     9121 tttcatcatt tatcctataa ttaaattttt ctgcctgttt tgccaagatt ttaattaatt
     9181 tttattagtt aaacttttat taatctcttc tgaaaatata ttcataaagc tctcaatagg
     9241 cattacgccg atgtctccta ttccttgacg acgtacagat agagcattgt cgttttgttc
     9301 tttttcgcct actataagca tataaggtat gtgctctaac tcagcatcac gtattttttt
     9361 gcctattttt tcagatctat catccatttc tgttcttaca tcaaaatttg agagcaaaga
     9421 tacaactttt tttccatatt caatatattt ttcactaata ggtaatacta taacttgtac
     9481 aggagctaac caaagtggta atttaccagc agtttgttct aataacaaag atacaaatct
     9541 ttccatagat ccgaaaatag ctctatgaat cattactgga cggtattttt ggttgtctgg
     9601 accaatatat tctaggttaa atctttcagg taagttataa tctacttgta ttgtgcccaa
     9661 ttgccagctg cgtccgatgg cgtcttgcac cataaaatct aatttaggac cataaaaagc
     9721 agcttcacct ttagcaatta ttatattaaa ttcggtatta gctacacttt ctaagattgc
     9781 ttcctctgct ttatcccaat tttcatctgt acctatatat ttttctttgt tatcaggatc
     9841 ccttagagaa acttgagctg taaaattttt aaaatcaaaa gtattaagta ttagcaaaac
     9901 tatttccatt actttatgaa actcttcatt tagttgatca ttagtacaaa atatgtgagc
     9961 atcatcttga gtaaaactcc gtactcgtgt gagaccgtga agttcgccac tctgctcata
    10021 tctgtaaaca gttccaaatt ctgctattct caatggcaaa tccttatatg atctgggttt
    10081 aaaagcatat atctcgcaat gatgagggca gttcattggt tttaacataa attcttcatt
    10141 ttcattagga gtagatatag gacgaaatga atctttagca tatttttcgt aatgtccaga
    10201 tgtcatatac agctcttttt gtccaatgtg cggtgtgatt acatgttgat acccatattg
    10261 aatttgtatt ttttttaaaa atttttcgag ttcctctcta actattgtac ctttgggaag
    10321 ccacattggt aagccttgac ctactttttg gttaaacata aataattcta attctttgcc
    10381 taatttccta tgatcacgct ttttagcttc ttctaaaaaa gttaaataat catctaataa
    10441 tttttttgaa ggaaaactaa taccataaat gcgtgtaagc atttgtctat gctcatcgcc
    10501 ccgccaataa gcacctgcaa tatttaataa tttaaaagtt ttaacatatt tagtagatgg
    10561 taaatgtgga ccacgacata aatcaatata ttctccaaga caatataagg taatttgctc
    10621 attttctagt tcttgaatta attcaagttt atatgtatca ttactttttt cgaataaatt
    10681 aatagcttca gcttttgtaa tctcttttct aataaagggt aaatcttgtt ttattatatc
    10741 ttttgcttta ttttctattt tttctatatc atcttctgtt attttataat caccaaaatc
    10801 aatatcataa taaaaacctt gctctatgct aggacctatg ccaaatttca catgagggaa
    10861 aagtgaaaat acagccgcag ccattatatg agcagatgaa tgccagaata cttctttgcc
    10921 ttctctatct tcccaagtca aaatttgtat tttagcatca ctttctattg gtctttctaa
    10981 atctaataac gtatcgttta ctttgatagc taaagcttct ctagctaaac tctgactaat
    11041 agattgggct atatccatac cagtaatgcc agcgggatat atttttttac tattatcaga
    11101 aaaagttata actatatctt tcatatctat ttaaaaaaat atttgcaaaa ttaaatcaaa
    11161 aatttgaaat aatttaatag tttattttat gttttacaaa aatgcaactt aaatttccac
    11221 tactttaatt aataaaatag gttctataaa gtcttgtatg agtaaataaa aattagtaat
    11281 ttttataaat aaaatgctaa tattgggcat attttttgaa tagtaaaggt aaaatcttaa
    11341 aagcatagat aacgataaga aaaaaaatgc tatcaatata tcaagctaaa attttattat
    11401 ctacaataaa ttttattgaa tctaaatgtt catattattt actttattat gtaaaacttt
    11461 ttcttaaatt tgtataaaat aaaaatcata atttagacta tctataaata atttactctt
    11521 aaatcttgtg tgatccttga catggttttt ataattttag taatataaat gttatgtttt
    11581 agaattgttt gtttttaatt aaaaatttta gataaaacta tcaaaaataa tgctttctat
    11641 tgttatttta aattataata cttttgattt tacctgtcag tgcattaaat ctatatatga
    11701 ctatacaaaa gatgtggatt tcgaaataat agttgtagat aatgcatcta ctactgatca
    11761 ggcagatagt tttttagaat tatttcctta tattaaatta gtaaaaaatt ataaaaataa
    11821 gggcttcgca ggtggatgta atgatggtat caaggtagct gaaggtgata caattttact
    11881 tattaattct gatacaaaac ttttaaacga tgctatatct atcacatata attttttaaa
    11941 aaaacatcca gaggtaggaa tagtcacgtg tagattagaa aatgaggatg gatctcctca
    12001 aaataattgt caaatatttc ctagaataag taaaattcta tttgaaattt ttagaattaa
    12061 taaaatgcta cctattaaaa aaacaggtaa aattttgtat ggtccctttt ttagttacac
    12121 agaaatagca tatcccgact gggtatgggg tacatttttt atgtttgata aaaaattgtt
    12181 agatattttt ccaaataaat tattaccaga aactttttgg atgtatatag aagatatgca
    12241 atggtgctgg ttagcaagtc aaaaaggcta tgaaatagtt tttgtacccg aagctcgagt
    12301 gttgcatttg gggggtaaaa atcatagtga aagagctaga gccatgatgg ataaaaatct
    12361 ctcacaattt ttatatcttt actataataa gatacatgca aaattaataa tttacctatc
    12421 aaaactttta gaactctcac agtggcgaaa ctaaaaagca aaatacacaa aaatctaaga
    12481 taaaaatata taattttcga aatatttgaa atcttcaata taattatttt aattataatt
    12541 caattttttt gattctataa cgtttattgt gggtaatcaa attttagatt gttacaaata
    12601 ggttattttt ttaccacaag agtacgaaga tattatataa agtcccattc ttctttgcgt
    12661 tctttgtgta ttctcagaga actttttggt tgatatttaa ctgtccaata ataaaaaaat
    12721 taataaatgt ttaagttttt caagtttata tgttaaataa tttttatatt tttacaaaaa
    12781 ttttaattca tgaaaaaaat tgtagaaaat accagtaaat accttgctat gagcaaggaa
    12841 gagaataaag aattcttaga caacctttat tcattaatga aaaaattaga agccgatgta
    12901 gtcgattatc aaggtctgaa aatactcagt gccccaccac tatgcccaga ctgtcgtagc
    12961 aatcacatca ttaaaaatgg tttacaaaat gggatgcaaa actatcgctg caaagactgt
    13021 ggcagacagt ttagagttac cacaggtact tttgtttaca ggttgcaaaa gaaagagaaa
    13081 atgctcgact acatcagatg tatggtagca ggcaaaagtc tcagagcttg tgctaaagaa
    13141 gtaggcatca gcctaccaac aagctttgct tggcgacaca agatattggc agctttacaa
    13201 agtttcgaag ataatatcaa ttttttcggt gtagtagaga tggacgaact attgatggat
    13261 tattccgaaa aaggtagaat atacaaaaat gccgaagagc tgaagcgagc taggaagcaa
    13321 aagccaaaga aagtagccgt attggctgct acagacagag ctggcaattt attattcaaa
    13381 aaatttgatg gcaaaaagct aagttcagaa caaatagagc aatttctgaa ggctaaaatg
    13441 ccaaaggatg gagtcatgtg tagcagtaat aaaaaagcat tcaaaaaggt tagaaggaat
    13501 gtaatcaagc acaaggaaat agtaactagc aataggcaaa agcgaggtat atatagccta
    13561 agtactgtca agaaaaaggc tagtgaattc acgaattgga tatatttcaa atttaggggt
    13621 gtagctacca aatacctgca aaactacatt atgtggtatg tagtgatggg taaatacctc
    13681 acaggcaaga ctgtaagcaa tactgggcga atgcttaacc ttgcatcctc ggacaggtgg
    13741 gcgtggtatc ggtatagaga gttagtgaat ataacatatg ttaatttaaa catatgttaa
    13801 ttttccgact gatccgtttc aagtttccaa ttctcctcat ccaacatatc taagattata
    13861 atttgggtaa tcaaattata gattgttaca aaaaaataac tatctttaat tatttaggta
    13921 atttttttat cacaaagtac catttgtagc ccttttaatt ccatactttt ctgtgttttt
    13981 tgtgcattct ttgagaactt tttggttgat atttaacagt cctataataa ataaaccaat
    14041 attaacaaat tactcttttt ttaatcttta cccatataaa acgttataga accatttttt
    14101 ttaattattt ttgtaaatgt atataaatta gaaaatggtt aatttttaat tttaaggtaa
    14161 taagttaaag tatgcataaa ttatatccat tcaaatttaa accccagttt tttcaaaaag
    14221 tatggggagg taaaaattta acaaaacttt atcatcttga aaacgatgat acattgggcg
    14281 aggcatggtt gctaagtagt atagaaggca aagaaagtga gataataaac ggtgagtggg
    14341 caggtaataa tattgtagag atgatcgagg tttttatgga tgatatcctt ggagaacata
    14401 ttttttatac atacggtgac acttttcctt tattaatcaa gctaatagat acttcggaat
    14461 ggctaagtat acaggtacat cccgatgata attgggctaa aaaattagaa aagcaacaaa
    14521 cttgtggcaa aaatgaacta tggtatttta taaaaactgc taataatgcg caaatattac
    14581 atggtttatc tcgtttagct gatcaagata ttataatgaa atacttaaat gaaaagaata
    14641 tagaaggaat ttttcgtaat ataaatgtaa gtccacatag ttatatttat acacctgcag
    14701 gtacagtaca tgcattgggg cctggattat ttttagcaga aatacagcaa actagtgaca
    14761 taacgtatcg tatctatgat tgggatagac ctcaggaccc aaaaaatcca agaccattac
    14821 acttagataa agctttcaaa gtaattaatt atgatgctat accagaagat ttacaatacc
    14881 aacctattat agctgataga actgttaatt tgtttaatgg agaaaatttt gtgattaata
    14941 gaatcgtttt atctagtggt aagcaaatag aaaaagatat tgaacctttt gatagtttct
    15001 ttgtttatct aattttgaat ggtaatggta aaataatttg tgatacttat gtagtagaaa
    15061 ttatggcagg tgattgttgt ctgataccag cttgtttttc taatgttata atacagtctg
    15121 ataaagatat tttagcttta gaagtatata tacaatgaac taatggtcat tttgtcaaaa
    15181 aactgactta gcaaaatctt aaatttatta ataaagtatt gatcttaaaa agttttgcaa
    15241 attaataaat taataaaaaa ttttttttaa ttaaaaagtt tatgtatctt tgcagagggg
    15301 taggtcctac acaaccagct cctacgaact cccccaggtc cgagaggaag caagggtaag
    15361 tggttgtagc ggtgtgatgt agggtgctta cccctatttt gattttattg ttttttctta
    15421 gttatgaaat tttttctaaa tacagtttac atagaagagg taagaaaaat aaaagaatta
    15481 caaataatag atggtatcaa tactaatctg gccgggatgg aagccctagg tgtaaaagat
    15541 ataaatcaaa ttttgactca ctataatgta atttgtcaga cttttaacaa aacagtatta
    15601 gtagatgtaa aagctaaaga ttattcaagt atattaagag aagtagatat attaacttcg
    15661 gtaaatgaat taataactgt aaagatacct gccaatttag atggtataaa agctatcaac
    15721 acattagcta atcaaaagtt tgatattgta tgtagttcga ttttttctac ttctcaagct
    15781 atcttggcgg ccgaagctgg agcactttat atttttatac caatgaaaaa agctatgcag
    15841 tttggaattg atactttgca tttattgact caaataatca atatatataa tgagcaaggt
    15901 tacgatactt atattattgc tgatggtatc agtagttcta tagaattaac tcaaatggct
    15961 ttattaggtg cagatggtat tagttgcgat atacaaacta tttattcttt ttttcaaaat
    16021 aatttttcta ataataaaat gtaaaatttt gttaataaat ttttttatat atttgaaatt
    16081 aaaaaaaaat aaaattatat gcttttaaaa attcatccag aaaatcctca acagagattg
    16141 atagttaaag tagtagatat actaaatgat ggtggtatag tcataatacc tacagataca
    16201 gtatatgctt ttgctactaa tcctttcaaa aaatctggcg tagaaaaatt gtgtatgcta
    16261 aaaaaattgg atcctagtaa ggctacattt tcattcttag gttctaattt atctattttg
    16321 agtgaataca caaagcctat tcctaatgaa atttttagat tgatgaaatc tgttttgcct
    16381 ggtccttata catttattct tcctataaat agtaaaatac ctaaaatttt agaaaatcgt
    16441 agaaaaacaa taggtatacg cataccggat aataaaatcg tacaggctat agtagatact
    16501 attggtcatc ctattgtctc ttcttctatt agggtagaag agcaactggg taatgaatat
    16561 atttctgatc ctgaattgat ttacgaaaga tacttagaat ttgtagatgc agtagtggat
    16621 ggcggtcctg gcagtttggt acctagtacc gtgatagatg caaccgaaga catgcctatt
    16681 ttgattagaa aagggttagg gcctgtagat tttttagatt taatagatga aggagaaaat
    16741 atttaatttt tttaatttta atgttaaaag aaaatctgag gaaatttttg aaaatatctt
    16801 tactaatgaa aatatcagaa tacaacgtat tttgtccttt ggacaggtaa gtccgccaga
    16861 ttattggtat gatcaagatg aagatgagtg ggttttactc ttaaaaggaa aggctaaatt
    16921 agaatttgaa aatgaaataa taacactcca tcgtggtgat agcctgctta tcccagctca
    16981 tatgaaacat agagtggctt atacgagtaa atattttact actatttggc ttgcagtgtt
    17041 tattaaaatt taaaaatgta actaaaagtt gaatttttaa attttttctt gtaattttgt
    17101 aaattataaa aaagtattat atatgagtat gataacaatt aataaaaata taacagaaga
    17161 taaactcgaa aaaataaaaa aatggcctat ttgggaaaaa gatatttctc gttttcctta
    17221 ttattatgat catactgagg aatgttatat catagaaggt agaataacaa tagaaactga
    17281 ggatggcata tatcatatag aaccaggtga ttttgttcgc ttcgaaaagg gactctcttg
    17341 tatatgggat attaaagaaa aagtacgcaa atattatgat tttccaaaat gaaatttaaa
    17401 aaaggcgata aagtaaaata cctcgatgag ataggtgaag gtatagtaaa agaaattcat
    17461 ggtgatctag taatagtaga ggatgacctc ggctttgaaa taccaatgaa agccaataag
    17521 ctcatattag tagaacactc aaaaaatatg aataatattg acgacactac tgatatgcaa
    17581 gataatatta tagaaggtga tacaaaaaca gatctatcta tattatctaa tcataataaa
    17641 tccaaaattc aacaatttac cgatggtatt tatctagtta taaaacctga aaatccaaag
    17701 gtacctatcg ctagtcctca tcaattgtat cttgtcaatt tcacctctta tgatgtttat
    17761 tttttattac atcaaataaa agttgggaat actaatattt atcacagttt tttaaatgat
    17821 tacactgcta ttaaattaga aaatcttaaa atagaggata tgcccaatga actcttatgt
    17881 accttgcaaa gtataataat atctaaagaa catatatttt taccttttat cagcaccttt
    17941 gctatcaaaa gatctacttt attaaaagaa gataattatt cagaaaatcc ttttttcgat
    18001 gagcctacaa tatcgatttt aataaaagac ttaaaagaaa taaacgaatt aaaaaatttt
    18061 gaaaaacaaa aagatgaatt agaaataaat ttttcaaaat caaaagaaag attaatcgta
    18121 gatgccagta taattgataa gtatttagta tcaccagagt tagcagaggt agatttacac
    18181 ttagaaaaat tagtagatga tccacaaaat atcagtcctg atatggcgtt aaacattcaa
    18241 ctatcatatt tcaaacaatg tttagatgcg gcaatttcaa aagatattga taagattatt
    18301 ttcattcatg gtgtaggggc aggtatttta aagagagaga tgtatcaaat cttaaaaaat
    18361 tatcctgatt tatattatgg agatgcgccc atacgtcaat atggtattgg tgctacagaa
    18421 gtttatatta aacctataaa aaaataaaaa ttatggcaaa aattcctatt gagagcattc
    18481 tgtttattga tgtagaaact gtgccaatag ctcctgatta tcaaaccctt gatgataatt
    18541 taaagtctca ttgggataaa aaagcaagtt ttttaactaa aaatgatgaa gatgatccat
    18601 catcagtatt tgaaagggca ggtatatatg ctgaatttgg taaaattgtt tgcatttcag
    18661 ccggtagagt gcaaaatgat gagatcaaaa cgaaatctta ttattcgcac aatgagaaac
    18721 agctattaga agaatttacc aatgatttga atattttcac tagaaacatt ccaaatcttt
    18781 atctatgtgc gcacaatggc aaagaatttg acttcccttt tattattcgt cgtatgataa
    18841 taaatcaaat tcccgttcca tatattttaa atactcgtaa taaatctaga tacgaattac
    18901 ctttcttaga cactatggaa atgtggaaat ttggtgatta taagcacttt acatcactcg
    18961 ctctattgtg tgaactcttc ggtattccaa cacctaaaaa tgatatagat ggtagtcaag
    19021 tagccagtgt atactataaa gaaaataact tagagagaat aaaagaatat tgtgaaaaag
    19081 atgtgtgtgc attgatagag gtatacaaaa ggttagattt ctaaaattat tttcaattca
    19141 attaaaagat tttttttaat tattttacta aaaatatgac tttatgattt tgcatgttaa
    19201 aaaagatata gataaaaatt tattaaataa tgttgtaaat tattataatg gtgttttact
    19261 agaaaaagat gaatatttta ttattattag ttcaaaaaat tcaaacatta agaatgaatg
    19321 gcaacaatat attattgaaa aatttgagtt gaattctgaa tgtcaattaa gctcgagaga
    19381 atacaaaaaa gaaactaaac gaatagattt gccttgcgga tatataggtg gagatgaaaa
    19441 aaataccatc ataatagctg gtccatgtgc tgtagagtct gaacagcaga tttttgaaag
    19501 tgcaaagttt atcaaatcat taggcattaa cgcattcaga gctggcttgt ataaatctag
    19561 atcttctcct tatacttttc aaggcttagg ggataaagga ttaaatcttt tatataaaat
    19621 taaaaatgaa tttaacttaa tcattgtaag tgaagtaaga gatattacta attttgacaa
    19681 aatagcaaat ctagcagata tattgcaagt aggagctaaa gccatgtatg atcaagctat
    19741 cctgagagct tgcggagaat caagtaaacc tgtattatta aaaaggcatt tcggggctaa
    19801 tatatctgat tttatacaag ctacagaatt tattttatct gctggtaatg aaaaagtaat
    19861 tttatgtgag cgaggtataa gaacattcga aactaaaact agatttactc ttgatctcac
    19921 tggtgtagca tatatgaaaa aatacttaaa tctaccggta gttgtagatc ctagtcatgc
    19981 gatgggatat tcatatggag taccagcatt aactaaagca tgtatggcta tgggaataga
    20041 cggattattg atagaagtgc atccatcacc tcaaaaagca ttgagtgatg ctaagcaaca
    20101 actaaatcat aaagaattta ctgatttaat ggtttcttta aaaaggatag caaaagcaat
    20161 cgacaagaat attatttaat aaaaaaagtt tttttatgtt agatatttta aaaaaaaata
    20221 tagaatattt tttagaacaa ttgccagatt tacaagatct aacaaaaggg aaagaagcat
    20281 atagtttatc agacataaga gctttagctg atttatttaa tgtgcccttt aattattgtt
    20341 ttaaatatga tttgagaaaa gaagatatac tgataaaaga ttttagtaat cgcattaaag
    20401 tcttagcttt agatgtagat ggtgtactca cagatgcagg tatgtatata gcttctaatg
    20461 aaaatgaaat aaaaaaattc aatgcacaag atggcatagc tatccagtat gctattaata
    20521 aagggatcat tgtgatttta ttaagctcag gactccgaga tgaaatcatt tcttatagag
    20581 cgagaatgct aaaaattcca cttttttacg taggtagaga atctaaatgg gataaattaa
    20641 ataatttttg taatgataat aatgtaaccc ccgaagatgt ggcttatata ggtgatgata
    20701 tcaacgattt atttgtttta gaaaatgtag gtttatcaat atgcccgcaa aatgcggttg
    20761 caaaagtaca caaagcggtt aaaatacatt tatttaaaaa tggaggtgaa ggggctgtac
    20821 gtgaattggt agaacgttta tatcaagatt tattcgatta attatgtatt ttttttcaaa
    20881 tattgccaat caaaataata aattaaacga agcgaaatag aattagtttg atcattttta
    20941 aataagtttg ataaattttc attaaaagaa ggcatcttag tataataatg aataatttca
    21001 ttagaaaaaa tttgattttt ccacataatg ttcatatatg aacctggggc aaaattccat
    21061 gaaaacataa aatccacatt aaaagcattg aaattaatat catgatcttg agcataatta
    21121 ttaataatag acaaactacc atcctgattt aaaaaataaa actcactata atctaccgtt
    21181 cgccaatagt gacgaatagt taaattaaaa tacattttgt tgtttagtac ccaacgacct
    21241 tctaatgagt tagtaatagt attcacatta cgtaagccaa aaattatctc atcattataa
    21301 atagtatcat ttacaaatcc gcaatcatta tgatcaaagt caaaatctaa accataaatt
    21361 agagtaattt tatcactaat tctcactcga ggttttatta gaccccacca tccgtttctt
    21421 ttgatgaaat ctaaataagc acctatgcga aaatcaagag caaaaacctt gcgatagtca
    21481 gtagaaagat aaatataagt actctgaata gctggacgtc tataaagtct acctggagct
    21541 ctagcttcga aataatcata atgatctgat aggctaaaat ctaaatatat ccaggtacta
    21601 aaaaatttat tagtctctag gtatgcattt aataaaagtt ttgtttgcac taaatcattg
    21661 cttttaaaca atctatgatg gtctaaatct gtatatatag acattttatt aaatttacca
    21721 aaaggttgaa agatattata gctcaaactc aaatggtgag ttataatatt attttgctgt
    21781 agatagccaa agtcgtttgg attgtagcga tcactcaata aatttaattc ataaacatat
    21841 tgccacttac catttaactt tcctaacccg ctattaatca taaatccatt atccaaatca
    21901 ttatttccga tactatcatt gatacagctc caagcagctt taccataaag tccaaatcta
    21961 ttcgatgggt ccataaattt aaaggcagta ccgatgacat tagacagata gttgatgtct
    22021 ggtctgatga caaatgcatt tgtcaaattt acataagaat tatgccaaaa actctgatct
    22081 aaaactacca tattataatt agcccatggc tcggtaagta ttttatgaat attacctgca
    22141 gagtccttta cttctgccca agtattagca gtaatagcat tgaaaactcc aataccaagt
    22201 tttttttcat tcctacctga aagtttaatt gcattgatga ggcgtgcata tctaggatta
    22261 tcaataatga cataattatc atctttcata gatctaatct catcatattt agcaggaatt
    22321 ttacctattc tacgagaata aaacaagcca actttttcga acaactctac tccttcggta
    22381 aagaaagggc gatattcgga ataataagtt tcgaaaggac ctaaatttag ctcttcttca
    22441 tcagatttaa tatgtgaaaa atcaggaaca agggtaagat ctaaagtatg acttttggtg
    22501 attcccaatt tcatatctag gccgccactt agaccatata ctggtttttt agcactagta
    22561 taaaaatcta tattagtcga gatataagga taaaatgcaa gtcgtatggg tggttttata
    22621 tctctgatcc caattatttt gcccatttgt tctatatcag gccctaactc ttgttttaca
    22681 ggattccaac agctatattc tctatttctt ctaattacac gccagaaatt agcagaccaa
    22741 gattgttcag gtatattagg aaatctaata gcagaataag gtatggctat ctctacggtc
    22801 catttgtttt catgaatcgt tacaccactc tgccatacag catcccaatt ataatcatag
    22861 ttgccatctg atatcataat gtcagtttgc acattggctg cagtcacttt aaattcataa
    22921 acattccttt tgtcattata tggatttaat tgtaaaataa acatatcagt attgccattg
    22981 tcctgatctc gcattgtcaa ttctttcagt attttatctg gctcatcatc atatagtatg
    23041 gcaccgatat acagagctct atcatcatat aatacacaaa cttttgttgg tttataaggc
    23101 atctgcccct gataaggttt ataagtaaca aaatctcccg tacattcggc ttttgaccat
    23161 gcctcttcat ttattaaacc atctattttg atttcttcat ttgtacgata tgctgttatc
    23221 tgcctttctt gagtaaaagc tgagattgaa aaaataaaaa acaaaatata aaatatatat
    23281 tccttcatat tactttaaaa tctaacattt aattacctaa tactactatg atataattct
    23341 aaaattttaa aaaatcaaat atgtcaaata tctatcattt aaaatcataa aactaatgtg
    23401 gttgttggtt attaaacatc agtcatttat ttttcctaaa ttattcaaat ttctcaataa
    23461 tattgataaa tctgtcatta catgataatc tctagcataa ttaaaatcta tctcatttga
    23521 tatatctgat tgtggattaa agcctaagcc atctaaagga gtgaaaacgc cttcgggtat
    23581 tttaggtaaa ttatgaaatt tgttttttgt gcaataacca acccatgttt ttttatttaa
    23641 tagcacacta ataatatttt taaaagcttg ccctgatttt ttaataaaaa gcatataaaa
    23701 aggaaaaaat attaaaagta taatactagt aaaaaaatca aaaacccttt ttcttcttcg
    23761 atttacaggt ttacctatgc tatttaattc atacaaaaat acatcttcag gatttaaaat
    23821 atatcttgag ctcagaagaa attcattagt aggtgaagat attctaaaat ctacatggtg
    23881 atctgataat gaactcatag aatcaattat tttagacatg gcaacactat tagtacaata
    23941 tactatttca ttcaatttgt atattctaat aatatcaatt aattggttga tattagcatg
    24001 ataaaaattt gaatcagata tatcctcagg atcaatataa aataattggt ctactgacac
    24061 attactctgt tctaaaacat tttttacagc ttttatatct tgctcatcac cacaaatagc
    24121 tattcgttta ttttgtttta ataatcccca agagtctaga tgcaaccatt cgattaaata
    24181 tcttattgct ataagagata ataaagccca tagtgtgcct aatagcaaaa tagctcggct
    24241 aaaccgatat tgttcattta ataaaccata agcaagtaaa agaattatac taccactgat
    24301 aataccagta aaaaaatgtt ttttgtgaaa tggtttgtca taaccaccat ttaaaaaaat
    24361 tgctattatc caaacaaggg aataaattaa taaggcataa tatagaaatg gcctagggta
    24421 aattatagaa tcataactaa agcgtatctt ttcccacaaa atagtaatgc catatagtcc
    24481 actatataat atcaatagat ctaggaaggg cagccatatt tttttgaata atcgttttaa
    24541 gaaagcaatg aaagctttag aataaatagc tatcgaaata aaaaatttca tccatttagc
    24601 acctttggta tataaatgtt tctgagtaaa aataagcata gcattataaa aattgtaaat
    24661 atagtttatg ctgccttttt ttgtgctttc tcccttgtaa tgtataatac gagtatgtgg
    24721 ataataataa tttttatatc ccgcttgcaa tattctataa ctaagatcta tatcttcacc
    24781 atacatgaaa aaagtttcgt ctagatatcc tatttcatct aatacctcct ttctgatgag
    24841 cataaaggct ccgctcagta tctctatttc attaatttca tcaggatcca aatgtcccat
    24901 gtaatattta gcaaaagttt tagaatgagg aaaaagtttt atcaatccac tcattttgca
    24961 aaatgacacc cagggtgtag gaatacccct tttagactca ggtaaatact gccctttacc
    25021 attaactaat ttgacaccta aggctcctgc atcgggatgt tgctccataa aagcaatcgt
    25081 tttagtaaat gtatctctct ctactatagt atcaggattt aataacaata catacttacc
    25141 ctttgctata ttcagagctt ggttattagc tttagagaag cccaaatttt cattattagc
    25201 tatcaaggta acttgtggaa atttttgctt aatcatagct acactaccat ctacactgct
    25261 attatctact actattacct ctccatctat gctttctaaa gcatcaaaaa cagatagtaa
    25321 acattgttct aaaaaatgtt ttacattata attaacaatg ataattgata gtgtcattca
    25381 aaaaaaataa aagtttattt taattatatt tttttagcaa aactatcttt taattttgta
    25441 atgagattaa agacaatgtg ctctgtatta ctatcgggat cagttacgaa atagcctaag
    25501 cgttcaaatt gataatgtgc tggtgtcaaa taattattta gggcaggctc accataagcg
    25561 gtggtagtta tcaaactatt aggatttaga taatcaatga aagtcttatc tgcttccaca
    25621 ttatctggat ctggtattgt aaataatttt tcatatagtc gcactacaat aggtatagca
    25681 tgctcggctg atacccaatg tattgtactt tttaccttgc gtccatcagg gctattaccg
    25741 cctctactag caggatcgta ctcaccgtag atctccacga tgttgccatt atcatctttt
    25801 ttgaatcctg tacatttaat gaagtatgcc caacgtagtc taacttcatt acctacataa
    25861 aaacgatgat attttttagt aggattttcc atgaaatctg aatgttctat gaagatttct
    25921 ttcgaaaaag gtaccaatct agtatcagac atgggatctt caggattatt taaagcttct
    25981 aaccattcta cttgattttc tggataattt gttatcacca gttttagtgg attgataacg
    26041 gccatacgtc gtggagcacg tttattcaaa tcttcacgta gacaatgttc taatagctca
    26101 tattctatta aattttccct tttagctata cctacaatct caataaacat ttttatactt
    26161 tcaggagtaa agccacgacg tctaaggcca gaaatagtag gcatacgagg gtcatcccat
    26221 ccacttacat agccaccttc cactagttgc aagagtttac gtttactcat aatagtatat
    26281 gatagattca atctagcaaa ttcgatttgt tgaggcgccc atatatttaa ttctttaatg
    26341 aaccattcat ataagggtct atgaacttca aattctaaag tacatagtga atgggtaatc
    26401 ttttctatcg aatcactctg gccgtgtgcc caatcataag tgggataaat gcaccattta
    26461 tctcctgtgc gtggatgatt ctcatacata atgcggtaca ttatgggatc tctcatatgc
    26521 atattgggac tattcatatc tatctttgcc ctcaatacgc gactaccagc agaatattct
    26581 ccagttttca ttttgttaaa taataataaa ttttcatcta tacttctatc tctatgttta
    26641 caaggtattc ctggttcagt aggtttagtt ctatttttgc taataagttc ttggggacaa
    26701 tcgcatacat aagccttacc tttttttatc aataatatgg cccactcata taattgttcg
    26761 aaataatcag atgcatagca ttctttgtcc cattgaaatc ccaaccattt cacatcttct
    26821 ttgatagagt ctatatattc tttattttct tttgatggat tagtatcatc aaagcgaaga
    26881 ttgcatgtac cattatattt ttttgctata ccaaagttga gacaaatact tttagcatgg
    26941 ccaatatgca gataaccatt aggctctggc ggaaaacgtg ttttcacagt ttcgaatcta
    27001 ccttctaata aatgtctgtc tataatctct tctatgaaat ttttattttc gtttctattt
    27061 tcttttgtat ttttgtttac taacatttaa attatttttt tgcaaattta ttaagaaaaa
    27121 tgacaactgt aatatattta aaaaatctca aatttcatgc atatatcggt tgtttctatg
    27181 aagaaaaatt aattggtcag ttatttgatc ttaatttaag gatagagcta gaccttacta
    27241 aacctatgat aacagataat ataaatgata ctataaacta tcaaaatgtt tataaaatta
    27301 tttctgagct aatgaaactt aaatataatt tgatagagca tgcagcatat catattttag
    27361 ataaattatg gcaaatttat cctaatattc aaaaaataga attagaaatt aataaattaa
    27421 acccaccaat aaaaggtgaa ataggtagtg cagcattctt tattagtatc gaaaaaaatg
    27481 aatttgaaca attaaaaaat ttacagatat aattaagaaa ataatctaac aactattata
    27541 gtgtgaaata aaatgagtta cgaataatta ttgacttgta tgcttaaata tatcctcttt
    27601 agatattata taaatttttt tctatcaaag gatataattt atcaatttgt ttttgtatat
    27661 ccattgtttt attaatagct gtgatgatct tacagtaata ttctggattg tctaaattga
    27721 gattttttct atctttcaaa aatttatata atacttgata gccaccaatg tgataattcc
    27781 acaattgggg gcttacatta gtgaaatatt tatcattatt tatataaatt cttttctttt
    27841 tttcattata ttctactttt tctattttat tagtaccaac ctcaccttca tattttgcta
    27901 ttacattatc caattcttta cttttcatta aatgtaaatc tacaagttta tttcctaatg
    27961 tagacatttt attaaaaagt ttataatttt tggcaaaagg aattctagga aaatctattt
    28021 tgagaaaatc tgcatatttt ttcctataga tattgctata aagtacagca tagacataga
    28081 atagaatctc cttgggagtt aaatgtttat tgtatgtttt atttaatttc tcataaatgg
    28141 atttatctat attaggttgg cgagtatgat attccactga tctttcgaaa agcatcatag
    28201 tgtgagcgga tggggtttta gtattatttt gttgttcggt gtataaataa agtggaaata
    28261 tttgagcggt acctggtgat tgtatttgat taatatcaat aatattattt gtaataaatg
    28321 catggcaaaa atcatcattc tttttgaaga caaatctagt tgttattaga cctaagtttt
    28381 cattaagcat atgactcatc aaattttttc tagttctggc tatgatatca tcatggtata
    28441 taatatatct aatatcaaat ggtcgatata atattttttt aatgcaactc atccaattat
    28501 cacattgcat gaaacgctct ctttgtttag ataaattcca ttgattatta tcttttatct
    28561 taaaagtttt tctgatagtt tcgtggtcaa tatttttatt tctaaatatt ctaatcttat
    28621 tttctaaagc ttcttttgta aaatctatag ctaaattatc gtttgcggtc acaataccga
    28681 caccatttac aggaaatatg tcatttattt tgtcccattt ctcataatat ttaatatctt
    28741 cgatgtttct gggaatgaaa aaatactctg gagaagcagg cctgatctct gtataatttt
    28801 ttttgtcaaa attattatct agtagccatt gatatttagc ttcacgcatg ccatagagat
    28861 ctttatgata aactcgacaa tcttctttat ttttttgttt tacaaatata gctataacta
    28921 caccttgcct aatgtcgaaa acattttcat ctttactacc atcagggcat ttttctttct
    28981 tcaaagcatt accatgtaaa tctaataagt aaatttcatt aaaagtattc atcagactct
    29041 gacgcatacc cctgaaagtc agattgtcta aataactatg attagttatc atacctacta
    29101 taccctggcc agctttatga attttacatt gagcaaatct gataaatttc acataatcgt
    29161 cttgtaacca caattttttt tcacctagtg gcatgccatc tactttgtaa taactcagtg
    29221 ctccattgat atcttttttt agtaattgct ctgtccattc attaatatta gcagatatgc
    29281 cactatatgg cggattgcct aaaatcacta aaataggttt gtctttttta acttcacaag
    29341 ctagatggct ctcttcgctc aatgaactca agccaggaat aattgtttga tctaaatttt
    29401 ctttttccaa agtattagtt aaatatagtt tgaagcgttc atcattactc attttataac
    29461 ctaattcatc aaaaagaaag cttatcttta gatgtcctat agcgtatggt gccatcatta
    29521 gctcaaaggc ataaaaattt tctagaatat gatttttgat aaaattttct ttgccaccag
    29581 taccatattt atcagtatat tcatttatag ccagtttaat agcttcggca gggaaagtga
    29641 gtgtgccagc agcaggatct aataatgtaa cttctttgcc agcaagccca tcagcaatat
    29701 cgaaatgagt ttttaaaata tcatttatcg aacgtacaat gtagttaact attggctctg
    29761 gagtataata tacacctcgt ttttttctaa ttttaggatc atattgtgag agaaaagtct
    29821 catagaaatg aactatagga tcctgacctt tgccatctaa ataaaaatct tgcaatattt
    29881 ttttgacatc tgttacctgc aatatctctg ctatatcatc tacaattatt tgcagtgatt
    29941 taggagcatc ttcgagagaa ataaagcgaa aaatatcgcg taaaatgcca atagtctgag
    30001 gaatgtattt aaaagctaat tctctattaa actcatcatt tgcccttgtg cgtgcagcaa
    30061 atagtccata agtgatagtt tgtgagtaca aatcagcaaa ggttttttct gttaaagtgc
    30121 ctatgagata tttctgaaaa gcttcataga atcggcgtat agctttttgt ccctttttta
    30181 attcttctgc catttctata ctcacaacct catcacgaag aaatcttgta cgattagcta
    30241 attctacagc taaattttta gcattgttaa ctttgggtaa agaaaaagaa aaaaatgtat
    30301 ccaataattc ataaaaatca tttgtattct ctactggtgg agctacattt aggtttttag
    30361 ctatagcata tctggctata gttgttgttt ttatcctatc accatttcta tatagtctaa
    30421 attcataaaa atttgtcaaa atcacattgg ggaaagtact aatgtatcgt tttaattgtt
    30481 ctgaattttc tattttatct agattggtta cagacggatc tttagcttct atgtaaccag
    30541 taatgtgatt tttcccgtcc cagacacgaa aatctggatt accagcctct gtttgcttgg
    30601 gcaaaatagt tatttcaact ttattataat tattttgccc tacgaaagcc aatagcaaat
    30661 cttttaaatg cccataatag ttctcctcac gagcatcacc tcgatttaat gtattttgta
    30721 tgcttgctaa atattgtttt agcatttaaa ttaatgaaat gtatattaat gataaagata
    30781 gttatttttt ataattaaaa aagaacaatt attttgataa tttatcaaaa taattataag
    30841 ttttaaatat tatttgttaa ttttgcttat ataactatgt ttaaaattta ttaaatatag
    30901 atataattag tatttaaaca ataaaaaaac gcaaattatg aaaaaattat ttttaattat
    30961 catttcaatt ttcagttttt tggttttaca aagtcaagta aataaccaag aaagtaaaaa
    31021 ttatagcgaa gatatcgcta cactaaaaaa atctatgact attgtacaaa gacaaattaa
    31081 gaaccaacaa ctaatcatct ctaatcaaaa taataaaata gatagcttaa ataaaataat
    31141 agcagtattg cataatgaaa tagaagaagg tagctctaat caagcaacta ttaatgaatc
    31201 cataaatgat ttaagtaaac gtattgtaaa tattaataat gcgttagcag aaaggaaaac
    31261 atatgtcatc ataggttcta ttattgctat cataatatta ttgttgattt tgtattactt
    31321 aagaaaaaat ttttcaaaaa ttaaatctac tatcaagcaa aaagagaatg agctaaatga
    31381 aagaataaca gaaatcaatg acaattttaa taatgaaata aataacgtta aaattttgat
    31441 agaaaataac aaaaatgaat ttaatgaaaa aataataaaa tttcaaaatg attttagaaa
    31501 taaccttgag actaatgtaa ataatttgca gagcttgata aatgaaaaat atcaattatt
    31561 aactaatgaa tttaacgaaa aattatcaaa tactattgat aatttaacta taactattaa
    31621 tgagataaaa gataactcga acaatttaaa atcatctgtt aataaagaaa taaatactat
    31681 acacacaatg ataacaaata taaacactaa tttggatgaa caaaaaaata atatatctga
    31741 gctagatagt aaattagttg ctataaattc agaattagat gttaaaatat cgaatttaca
    31801 aaaagaatta gaaaataata tcattgagat aaatgaaaag ataaataatc tactaaaacc
    31861 gacccagaat aagtagaaac gatagatagg aggtaattat aattacctcc tatctatctc
    31921 tatcattgta tttttctttc aatttatttt tcaattcatt taattgctca tttaattctt
    31981 ctttattttt tcttttttgc tcatttatcc atttatatct tttcacaaat ggtgaaagtt
    32041 tttgatgacg attaattaga tgaatagcga caatcaaata cgtggcaaca aataaaatag
    32101 ctattgatag attgatgggg tatttaggca ctaacattaa taaatagtca taaaagccat
    32161 gcgatccaaa agtaatcata aaaccaataa acatcagttt attttttctc tttactaaat
    32221 taaatctagc ataaccaaaa taataaccca tagctacagc aaaaacaaaa tgtgctggtg
    32281 taggcatgat ggctctcata agggcaacag tccatatatg agacaaatca ttaaaaataa
    32341 aagccaaatt ttctaagaaa gcaaacccca ttccaatagc taccatataa accaccccat
    32401 ctattggttc atcaaagtat ttttttggcc agataaacca cctaaaaaat aaaaatttta
    32461 ttccttcttc tattaaagct acaaaaaaaa atgcttctat aaatgcactg agttctaatg
    32521 gtaaatttag tttgatattc gaaaaaagca tattttctaa aaatgaagct ggtatagtgg
    32581 ctagcatacc aagaaaaaat gcaaaaaata aatatttaaa aggctctggc ttgtatttat
    32641 ctttgaaata tactaaaagt aagaaaaaaa gtgcaggtat tagtgctaaa atgatttcta
    32701 acatttttat ttatttatgc aaaaataata actataatga gaatttgata aaaaaataat
    32761 tacataaata aaaatactaa aagaggagtt agaggtttag aaaattgcat attgtaattt
    32821 cttgatattt atagggacaa tttaattttt ttttaaatta aatatagttt gtaaatatat
    32881 cataactatt ttttcttaac ttattacagt taaaatcaat ttacgtgatg aggcatgatt
    32941 tctaaattca caaagataga tgccttgcca tgtacctaat gctaattgat aattaactat
    33001 tggaatagaa agtgatacac caaaaataga acttttgaaa tgagcgggca tatcatcact
    33061 gccttctata ttgtgagtaa tttctggtaa attttcggga attaatttat taattatagt
    33121 ttccatatct acacgtacat cagggtcgta attttcattt atagtaagag cagcggatgt
    33181 atgttgcaaa aaaatattta agagtcccat ctctggtaaa ggtttaatag cttctacaat
    33241 tatagaagtt attaaatgaa atcctctttt gtgagaagga agtgtaagct ctattgtcga
    33301 tatcataaat caaacctcct tgtctttttt tttaagatta tttaaatatt taagttttct
    33361 tagttctatt ttttctgtaa tagttttttg atattttata tttttttcta ctaatatttc
    33421 tttagatatg tacttaataa atattatttt attattatta aattatcaat aataaaatgc
    33481 ttttctccag taatagaact aacaaaatta atagctatgg tgtatacacc ctcaggtact
    33541 gtttcacctt tatatgtacc gtcccatcct tgatttatat cggtagtata gaaaagttgc
    33601 ttgccatatc tatcaaaaat ccgcatttcg aagttaattg gatttatttc agcacctaca
    33661 aagttaaaaa cttcattcaa atcatcatta ttaggtgtaa aagcatttgg taaataaaaa
    33721 gtagtaatat ctttaataaa cacattagca ccaacggtat caatgcatcc atattgatct
    33781 tcagcatata agattacatg atatgtacct acatcccaaa aattatgtat tggagcaaat
    33841 tcagtggatg tatgtccgtc tccgaaatcc cagtaatatg aaatagcacc aatagaaaaa
    33901 ttttcaaaat atattgttgg attatttatg gtagtaactt gtggtctagg tgcaaaattt
    33961 gcctttggac cagggagatc atttactgtg attttagctg tagctgtgca tccattgtaa
    34021 tcagacactg taacttggta gttaccactt gtaatgttat taagagtagg agaactatta
    34081 ccatttgacc aaatataaat ataaggttaa aaatttaact tcaaaaattt cgtttctact
    34141 ttattaccat ttgaccaaat ataaatataa ggaggtgtcg ctccagttac atttagtgtt
    34201 atgctagcat ccgatttgcc acaat
//
LOCUS       DAOPRN010000015        33753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1010_length_33753_cov_103.799543, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000015 DAOPRN010000000
VERSION     DAOPRN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33753
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_33753_cov_103.799543"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..549
                     /locus_tag="PKI65_02950"
     CDS             175..549
                     /locus_tag="PKI65_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOB77433.1"
                     /translation="MSKINNHQELEVYQLAFTNAMEIFNLSNNFPRTELYSLTDQIRR
                     SSRSVCANIAEAFRKRRYRKAFIAKLSDAESEAAETQVWLDFAFNCNYIDENIKDYLH
                     LQYDFIIGKLVNMINNSDKWSL"
     gene            684..2072
                     /gene="lpdA"
                     /locus_tag="PKI65_02955"
     CDS             684..2072
                     /gene="lpdA"
                     /locus_tag="PKI65_02955"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOB77434.1"
                     /translation="MYDLIVIGSGPGGYVAAIRAAQLGMQVLVVEKENLGGTCLNWGC
                     IPTKALLRSAHIYREIISANNYGIKINEPPLIDMPQIVKRSRDIASMMNKSIDFLFKK
                     NNVNLARGVAKLLSNHNVEVKDVDDNVNIYEAKNIIIATGSRSRQISALPQDGKFIIN
                     YKDALILDHLPKEMVVVGSGAIGMEFADFYSSLGVNVTIIEFLDRLLPLEDQEISKTM
                     ERIFRKKKINVLTSSEVLKSEIKNKKIFLTVHTPKGIQNIETEMVLSAVGIQANIENI
                     GLEDIGIRTNKGKIVVDDFYRTNIDGYFAIGDVIDTPALAHLASKEAMICVDKIAGKQ
                     VNPLDYNFFPSCIYTNPEVASFGYREHEAINDGFNIKIGKFPFTANGKAKANGLLDGM
                     IKLIFDVSTDRLIGAHLIGENTTEMIAELVVSHNLNATAKDIYESIHPHPSLSESIME
                     AAAAAYNEAIHI"
     gene            complement(2177..2668)
                     /locus_tag="PKI65_02960"
     CDS             complement(2177..2668)
                     /locus_tag="PKI65_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOB77435.1"
                     /translation="MGTKGKSIVKLDVDKLIEMLNKAYADEWLAYYQYWIGAKVVKGA
                     MRAEVEGELVEHANEELEHAEKLAERILQLGGTPLLKPEDWYKMTNCGYDAPENPDVK
                     IILQQNIKGEQCAIEVYNKLLEFIGNNDPITYHLICEILEDEVEHEDDLENIMEDIES
                     MIK"
     gene            3093..4616
                     /gene="guaA"
                     /locus_tag="PKI65_02965"
     CDS             3093..4616
                     /gene="guaA"
                     /locus_tag="PKI65_02965"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOB77436.1"
                     /translation="MEKIIIINNGSQYTQLIARKLREFNVYCEILPWNQAIEVTNDIK
                     GIILSGSQYSINDPNAPTPNLDDFIGKVPILAICFGAQYIAKYYGGEFIENLTSECGN
                     AKLKYIDNSSPIFKNIYPPKIVFITHSDTIVKIPKHFKIICSTDNEEIAGFQISKNKI
                     YGLLFHPEHPQTEISDDIFRNFLAVCKVSYDWTPNIFIEKKINEIKEIVGNDEVIIGL
                     SGGVDSTVTAILIQKAIGNRLHGFFIDTGLLRKNEFEDVLNSYKKMGINVKGIDAKDI
                     FLSRLKGIIDPEEKRKIIGMTFIEIFENEAKKIKNVRWLAQGTIYTDVIESGSVNGLS
                     KTIKSHHNVGGLPDKLNLKLLEPIRMLFKDEVRKIGSVLCIPLDILNRHPFPGPGLAV
                     RILGEVTYEKVKLLQDADDIYISMLKENNFYNKIWQAFAVLLPVKTVGISGDERSYDY
                     AIAIRAINSIDGMTANAFDLPFDFLNKLANEITSKVNGVNRIVYDLSPKPPATIEWE"
     gene            4782..5705
                     /locus_tag="PKI65_02970"
     CDS             4782..5705
                     /locus_tag="PKI65_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOB77437.1"
                     /translation="MVNFGDTKVQRYSRLELRARQLVAGYITGIHRSPFHGFSVEFSE
                     YRPYNPGESTRFIDWKLYARTDKLFVKRFEDETNLRARIIIDASNSMLYPMNEYGEEE
                     LRSKYHMSMYMTAVFLLLFYQQRDAAGISLVSDEILEHTHARSSYTHHRFLISILDKY
                     LLQIRQLPKTETKLSKNIHEIAERIHKHSLVIVISDLFEANLDTQPLINALQHLRYNN
                     NEVIVFHLLDKPIEWNFDLKNRPYLFTDMETGEKIKIHPRSYKDLYKEKIKDYHHQLK
                     VNLLQLGIDYNLVYLQDGLEGILLPFFMKRM"
     gene            complement(5797..6636)
                     /gene="gldN"
                     /locus_tag="PKI65_02975"
     CDS             complement(5797..6636)
                     /gene="gldN"
                     /locus_tag="PKI65_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOB77438.1"
                     /translation="MKYLSLIVTFLILSVITLQGQVIEAPTDGAYEMDDLHNRVPVPY
                     QFIHESDVLYAKRIWREIDMREKINHPLYYPIQPTNRRYSLMSVIMIGVQEGGITPFS
                     PESDDFKVPMTKETVQNIIRREETTKTYDPFTGEERDTVIVTEFDPSTVTRYRLKEDW
                     IFDKNRSVLDVRIIGILPLMVEFEPDGITEKGTRPLFWISFAQLRPYLVRYEYFNRKN
                     DGQRLTYDDAFNKRMFGSYIIKESNVYDRYISSYTTGIDALLESERIKNDIFIFEHDL
                     WEY"
     gene            complement(6651..8264)
                     /gene="gldM"
                     /locus_tag="PKI65_02980"
     CDS             complement(6651..8264)
                     /gene="gldM"
                     /locus_tag="PKI65_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOB77439.1"
                     /translation="MGAKNCAETPRQKMIGMMYLVLTAMLALNVSKDILDAFIIVNDS
                     IEETNRIFYDKVQGNYAMFDKAYAMTPEKVKDNYEKALIVKKEADELVKYIEDLKAQV
                     IAVTERVSVEEARKLNLRDIKGRDNFDAPTNFFVGSSQDGSAGKARELKNKLVGFRKQ
                     MIELLGKYADKVQLGLDIENKMYPTIEGDQQLNWEMTNFYHTILAADIVILNKLILEV
                     RNMEADVVAQLYSAVSAEDFTFDRVGVKVVPKSSIVVAGSQYEAEIFVAAYDTRQNPE
                     ILIGDDVDTVNLKVLGNVTKLEGDSGRGILRIPATSPGAKKFGGTITIKSQSGAVQTY
                     WFKEEYFVMSPTATVSADKMNVFYIGVDNPVSISVPGVPNDRVRASITNGTIIPKGNG
                     KYIVRVSQGTKSVVNVTAEMNGKMMSMGSAEFRVKRVPSPIAKVAGKAGGNYTKTELL
                     ANPYVIADIENFEFDLKFDIVSYSFTYRTPAGDLLDKQVSGMRFTQDVIDIIQRSTRG
                     SRFYIENIKAKGPDGTVRELPTVSMRITG"
     gene            complement(8281..9369)
                     /gene="gldL"
                     /locus_tag="PKI65_02985"
     CDS             complement(8281..9369)
                     /gene="gldL"
                     /locus_tag="PKI65_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOB77440.1"
                     /translation="MGWLDKIVKSRAYKNFMTKLYGIGASLVILGALFKITHWPGATI
                     MLSIGMITESVIFFFSAFEEQPVIPDWSLVYPELWGYYHPDEPMDEEMLKKIEARKAA
                     EALGTGGEIVVKDSVTQKLDKMLEEAKIGPELIESLAEGMRNLSENAKKLSGLADATT
                     ATDNFVTNLNTTANTVAELNQTYSQTKEATAKVIGANLEFADNLKNIVSNFQKLSQAT
                     QTSASTMEETAQVNKIYAETIQNATNSANQLAKTYIESSEMLVNSTAKLDFQGIDNVK
                     LREQFEKITNNLAALNSIYEVQLNEMQNQLSKVKSFGEGVEDFTTNVQEMLVSIQEYK
                     SQTQKLTLNLQSLNTVYGNMLAAMSINR"
     gene            complement(9407..10810)
                     /locus_tag="PKI65_02990"
     CDS             complement(9407..10810)
                     /locus_tag="PKI65_02990"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOB77441.1"
                     /translation="MIKMDIKQSKASLQILGLVCVMALVVSSCDTSDKGQLLGVRDRP
                     AWYQPTPYGMVFIPLGSFIMGQSDEDVPYNHIQTNKVVSVQSFYMDETEITNNEYRQF
                     VYWVRDSIAHTILGEIDESHLITQNEYGEEIFPPAINWKTKINWEGQEEREALADMYL
                     PEHERYFRKKQFDVRKWNYEYYWIDYRAAAAKNWDSPLTSDQTDNVTMPMWNNRPQGY
                     QDRSVYVKKDVINVFPDTLCWIHDFTYSFNEPMTQSYFWHPAYDHYPVVGVNWKQARA
                     FSIWRTNYLNSYLSKTGSAYVNDFRLPTETEWEYAARGGLQLSPYPWGGPYTRNYNGC
                     FLANFKPLRGNYVDDGGLHTVIVAHYSPNDYGLYDMAGNVSEWTNNAFDPSAYQFAHD
                     LNMDYSYEAKDTDPPVLKRKVIRGGSWKDISFYMQVSTRDYEYQDSAKSYIGFRNVQT
                     YLGRHKDDGPMSSNVYR"
     gene            complement(10862..11857)
                     /locus_tag="PKI65_02995"
     CDS             complement(10862..11857)
                     /locus_tag="PKI65_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOB77442.1"
                     /translation="MKRQLYFIIIFLVMVMSSTVLKAQQEAQFTHYFQNFNAYNPGFA
                     GVKNSICATALYRNQWVGFKEVVNGETYTVNPRTMLLAVDGYIKALHGGLNLIVYNDK
                     LGYENNTGVKIGYSYNFYIRDFTLGVGFNLHFLDKSLDFSKLNPLEPNDPLLMQRSKQ
                     SDMVIDGDLGVFLLKPNQFWVGLSAVNLLQSKAELAEASNLKLKTHLFLTGGYIFPLN
                     NDQFELRPSALIKTDFTTVQYDINLQVYYQGMVWGGLGYRVHDAVMINIGAQPFANSL
                     NQGLAPISFGYSYDFTTSAIGRNKVSSGSHEIFINYCFLIEKKTYRSGYRNVKFL"
     gene            complement(12527..13819)
                     /locus_tag="PKI65_03000"
     CDS             complement(12527..13819)
                     /locus_tag="PKI65_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOB77443.1"
                     /translation="MYKSLDRVSERRSLLSIGILFFVFGFFTWLNSILIPYFRIICEI
                     GWFEAFLVTTAFYISYFLLAIPSAILLEKTSLIKGILLGLGLIVIGTLVFIPAALSRS
                     FPIFLLGLFLQGAGLTLMQTASNLYVTLLGSIDSAAKRISLMGIANKIAGALGGLILG
                     GVLFGDTKPQEIQNFLSQLGGSERINYLDQLAHSIISPYLIMSILLIALMFIFYAMKP
                     VETRAKTQKHKYSFKGIPKHSWLGFVAIFLYVGAEVIAGDSSVAYAQSTNLQPLLLCI
                     GNFCIDFSTPHYFTSYVMTGMIIGYLLGIILIPKYISQEKMLKIFSIIGIIITIAILL
                     LPTHLSVYTVPLLGFAHSIMWPCIWPIALRNADEKAEMVSALLIMGIIGGAILGPFFG
                     WLCDKIDMQYAYSILIVSYLYLIFYSRVGERVRQSETN"
     gene            complement(14317..17034)
                     /locus_tag="PKI65_03005"
     CDS             complement(14317..17034)
                     /locus_tag="PKI65_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOB77444.1"
                     /translation="MNDKQLTTEKDQNYWLKKLLKVLLIVIIVIILFFISSFIYVYTH
                     QDIIKDKAVEYVNKHIKVPLHVNTIKLNLFSHFPQVSVRFTEVEICEKELLSLSYLDL
                     SINIWDIIRGKYNIKKIYLYQGNISLKVMEDGSNNFDIFVKDTTTKASPILLEFPNIS
                     LNDIHLSYHSLKDQFYIGATINKMLTKGEYKKEVFNETSSIKLVLDSLIIGSDFCIRQ
                     PININLSTNSSIDFKNSIFNANKISANINGIDLLLALNLNFNKKNPTIHSDINIKKTL
                     LSNIITILPENFQENLNKFKLKGTLGLSAQIKGSLAKEKNPKLDALITLNNAEITNEN
                     FNFSNINLIANINSKKLFDLSVYNVEINNLKAKYGRNSNLEGKAKIYNLIAPQIDIAV
                     NGILNVEELKFINSESINLIKGSVQYKINGSANIPNWNVGYKSILALAHLNAKFDLQT
                     VDFFSSSYNFNNVNATIIINDDSLILNPLNCNINHQQIQNANAIIVKWKNLLLSTQPQ
                     LIINLKANIPSMVLDSLINTSSPQKAKTTKDKSKVKPTILANADININNFLYKTWVAK
                     NVKINSEIESRSKHVKININSDLIRHKLFTAGDLKINGRMISDKFIIDNIDFKAFSGN
                     INGSGYVVLSDKQNEFVIDLIAKQININQLFREMENFNQTLVTNKNIKGIADIDLQAR
                     GSTTKDWKLIKPSIKATANVNIKNGELINFEPLIGLNKFIKRDFSYIKFGELKNTFYL
                     SDQIIEIPQMYILSNVINIKVSGSHTLDNEIDYHLAVKARELLHHSATQINYIDDYGY
                     INYDDDRGMTLYVRIHGKPGNYKYSLFDKEQRKQSREETFKIEQQSIKTNIKEEMHLF
                     EKDTSLFIIKPGDEKAKTFKYDWIPADTINETKTKTIEKPKKKFTFDWD"
     gene            17195..17563
                     /locus_tag="PKI65_03010"
     CDS             17195..17563
                     /locus_tag="PKI65_03010"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HOB77445.1"
                     /translation="MDFSDLLFGLTRGFGLFINLLILALYIISLWIIFQKAGKPGWSA
                     IIPIYNIIVYLEIIKKPWWWIFLFLIPVVNLVFYVIVNHQLAIYFGKDAGFTVGLIFL
                     PFIFYPILAFSNAQYNNSDN"
     gene            complement(17779..18456)
                     /locus_tag="PKI65_03015"
     CDS             complement(17779..18456)
                     /locus_tag="PKI65_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOB77446.1"
                     /translation="MEPKKNPKKDLNRKRTLFLQIGLVISLTIVFVAFEWKTYEKQED
                     ILTATGPVQIDQEIVQITQQEQPEPPKAPQVTTILEVVGDDEIIQDEIDISIEDRPDQ
                     AAQTYVPVLVEEAPEPVAEEIFTFVEEYPEFPGGEKALYEYIKNNIRYPEVARTSGIT
                     GTVYVQFVVEKDGSISDVKVLRGIGGGCDEEAVRVVKSMPKWKPGKQRGQPVRVYYTL
                     PIDFKLM"
     gene            complement(18520..18897)
                     /gene="gcvH"
                     /locus_tag="PKI65_03020"
     CDS             complement(18520..18897)
                     /gene="gcvH"
                     /locus_tag="PKI65_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOB77447.1"
                     /translation="MNIPENLKYAKTHEWVRVEGEYAYIGISDFAQSELGDIVYVDIP
                     TEGETLKQGDVLGTIEAVKTVTDIYLPISGEIVEFNQTLTTNPELINKDPYGDGWIVK
                     IKISDPTEVEKLLSAVEYKSAIQ"
     gene            complement(19066..19422)
                     /locus_tag="PKI65_03025"
     CDS             complement(19066..19422)
                     /locus_tag="PKI65_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOB77448.1"
                     /translation="MNHNQIYGQNINIDSLDLLKSPCTLLLFTKSNCPYCKMQRSFFD
                     SLTIDYVIVDINKYEKWSNIFQISATPTLLIISNGLAIKKYVGLCKNKCWQDFLNETC
                     IENKNQKINKMSNQKN"
     gene            complement(19463..21226)
                     /gene="sppA"
                     /locus_tag="PKI65_03030"
     CDS             complement(19463..21226)
                     /gene="sppA"
                     /locus_tag="PKI65_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOB77449.1"
                     /translation="MSTFSKNLLANLLGNLITLIIFFILLFFFIMGMISLSVKSVKKT
                     SEPTIEYNVLKISLATPIKECEKDNIIYYGDGKIQKQLSSYDVVNAINYAIKDDKVKG
                     IILDCSEVNSDLTTLRPIKMALDSFKRSGKFVYSYSNNYTQTGYYVATASDKIALSPI
                     GSILWKGVYLEINFYKNLLQKMGVEMIVLREGKYKGAVEPYIQDKMSPENREQYTHYA
                     NQIWNTIITEVSQARNIPIATLNLIADSLYALNAQYTYNNGMVDTLMTSIEFNNWVEN
                     DIGNRVKPITIGKYIDQNKANIKIDKKTINKIAIVFAEGEIMMKGSLDGKITAEKMVP
                     LLRKLAEDDNVKAVVLRINSPGGDAVASDVIWHELMNIRSRKPLVISMGSYAASGGYY
                     LSSAGNYIFAEPTTLTGSIGVFGLYPVLDNLLNKKLGISQDGVGTNANSDFVSSLKRP
                     NNYAIKMLQKEISNFYNTFLNRINQGRGLSISYIDSIGMGRIWTGIDGVRLKLVDSLG
                     NINDAIKKAVRLSKIEEYRIVTYPAPIGLYQKLMDMMSSDAIVNDVIAEELKSFYPYY
                     LINNIENPIILTRMPYDINFK"
     gene            complement(21392..21919)
                     /locus_tag="PKI65_03035"
     CDS             complement(21392..21919)
                     /locus_tag="PKI65_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77450.1"
                     /translation="MDTILLIDTIIEFQQNLNYKDIYSQFSFSYLTNLLSLLSTPIDD
                     DNYEKLLYKTSMLSPNRELLFCILKNYLQNTNKSTNKINKYSNIIDEFIKKDPKIVLP
                     PKDDLPENIDDLTANIKVNDDDFVSETFACIFTKQKNFDKAIEIYEKLKFRNPEKKDF
                     YQEKIDELIKLKTQV"
     gene            complement(21923..22429)
                     /locus_tag="PKI65_03040"
     CDS             complement(21923..22429)
                     /locus_tag="PKI65_03040"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOB77451.1"
                     /translation="MNKFNTSFIFIGSIILLTACVRFTFSGASIPEGAKTFTVKYIVN
                     NAPLVNPTLSQLLTDKLRERIQSQTNLIMVNDGGDLTFEGEITNYVVQPAALQANDVA
                     ALNQLSITVSIRFDNKIDEKQNFSQQFSRYQQFSSSVTLSSVEDMLVEQIVDDLIDDI
                     FNRAFINW"
     gene            complement(22426..23709)
                     /locus_tag="PKI65_03045"
     CDS             complement(22426..23709)
                     /locus_tag="PKI65_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOB77452.1"
                     /translation="MDELYLIKQRFGIIGNSPLLNHAIEVAIQVAPTDLTVLIIGESG
                     TGKEFFPKIIHTYSARKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAIDSRKGYFE
                     VANGGTIFLDEVGDLPLSTQVRLLRVLETGEFMRVGSSKVMKTDVRVVAATNANLQEA
                     IAKGKFREDLFYRLSTIPIYIKPLRERKEDIPLLFQKFSTDIAEKYNVPPITLTDDAL
                     EMLINYKWPGNVRQLKNVAEQMTIIEKNRKINAETLKKYLPEEKLNLPILLSELSDKD
                     SISERELIYKYLIDMRRDLNEIKKIVFKYLLSNQPIDADTAQKINDMSVSSLPIKIIE
                     QDSYQSINDFRIDEHSINKFKDKTEDAVAYVVEDSVEDSNDDSNENLSLQDNEKELIV
                     RAIKKHNGRRKLAAEELGISERTLYRKIKEYNLEV"
     gene            23826..24257
                     /locus_tag="PKI65_03050"
     CDS             23826..24257
                     /locus_tag="PKI65_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77453.1"
                     /translation="MKKNNLIIVAVITTFLISTSCSKYDEGPLISFRSKTDRLCQTWK
                     PKKILINGAETILTPDQQKTTIEFKKDGDLYLTTYRLSIAFPLTGTWKFYDKDEKLIL
                     SIDKGDPEPQVDTVKILRLMKDELWLKQKKDNIIEIHLEPK"
     gene            complement(24275..24964)
                     /locus_tag="PKI65_03055"
     CDS             complement(24275..24964)
                     /locus_tag="PKI65_03055"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOB77454.1"
                     /translation="MKERIIKLGNRILKDTLETQGMRNKLINEIQRKGINDEAVLKAM
                     REIPRHFFLPAGLEHFAYLDKPFDIGEGQTISQPYTVAFQTELLDVKPDMKVLEIGTG
                     SGYQAAVLAQMGAQVFTIERILNLHLNAKDILSALGYNNIQCFLGDGYEGLPQYAPYD
                     RIIITAAVDQIPDSLKDQLKIGGIIVAPVSYTLGTQVMTRLLKEDENNFKEEKHGFFT
                     FVPMLPGIKKN"
     gene            complement(25145..25236)
                     /locus_tag="PKI65_03060"
     tRNA            complement(25145..25236)
                     /locus_tag="PKI65_03060"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25200..25202),aa:Ser,seq:tga)
     gene            complement(25338..25868)
                     /gene="rsmD"
                     /locus_tag="PKI65_03065"
     CDS             complement(25338..25868)
                     /gene="rsmD"
                     /locus_tag="PKI65_03065"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOB77455.1"
                     /translation="MRIIGGFHKKRQITLPSKIDISPTTDFAKEALFNILRYYTDFID
                     IDVLDLFSGTGNLSYEFASRGAKNVIAIEKDEFWYKFILQNIEKIKLQSISVYQVDAF
                     QYIANSYDRFDIIFADPPYNIENIEKIPDLIFDNKLLNDEGLLIIEHSAKISFQNHPR
                     LIDHRAYGKVNFSFFQ"
     gene            complement(25856..26605)
                     /locus_tag="PKI65_03070"
     CDS             complement(25856..26605)
                     /locus_tag="PKI65_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOB77456.1"
                     /translation="MSLKNFEKIFYSELEINNIVLSLFAEGAYAIYLNDKKPIKINSL
                     PWDTFDDNLLKELNNITKFNLIISSTQSTILPDEYLLKYSKNDFATILGWENTDDIKM
                     NSIPALNAIMLNKKINTPDQINIYPYTPHEFLWIQYILDKNEDGVFIDIHTNTMIIAI
                     VCEGKLLFYNLLEYKTPMDMLYYVSSAINVVGLSDTSLPMYFSGAITEDSHLIEKVDP
                     FFSEIKLLSHSTNILGEFTHFFISSIKLCEL"
     gene            complement(26605..27201)
                     /locus_tag="PKI65_03075"
     CDS             complement(26605..27201)
                     /locus_tag="PKI65_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77457.1"
                     /translation="MKTTKLIVQIVLWVVIIVLAIMIVKSISDPLNFQREFNKRQEKV
                     IEKLKQIRSAEQMYLQVNNRYTANWDSLADFIENGKIPIVKLTADPKDTTFSVFIRDT
                     IGYKIVKDSLFKNIPNFNPKNLKYIPYSDITGTPKDTFIIRTDRINRGNVWVNTIEVK
                     APYKSFMKDYDLEKYGIDAEGFLKFGSITEPITDGNWE"
     gene            complement(27392..28900)
                     /gene="gltX"
                     /locus_tag="PKI65_03080"
     CDS             complement(27392..28900)
                     /gene="gltX"
                     /locus_tag="PKI65_03080"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOB77458.1"
                     /translation="MVRVRFAPSPTGPLHIGGVRTALYNYFFARKNKGKFILRIEDTD
                     QSRYVSGAEEYILESLAWCGIQFDEGINIGGEYGPYRQSDRTGIYREYINILIQKGAA
                     YYAFETPDELNQIRKEYESRGETFQYGINNRMNLKNSLSLPASEVKKLLDTSHPHVIR
                     FKVPEAKEVTVNDLIRDEIKVNSIIIDDKVLFKSDGMPTYHLANIVDDHLMKISHVIR
                     GEEWLPSLPLHVLIYQAFDWEVPEFAHLPLILRPDGKGKLSKRDGDRLGFSVYPISWQ
                     DPKTKEIYRGFREDGYYSEAFVNMLALLGWNPGDDREIMSMEEMIEAFDLKDVHKSGA
                     RFDPIKARWFNHVYLQKRDIKLVAQEFKDELSKVNINVTLEEAVFVAELMRERVDFVK
                     DLVTEAIFFFKTPETLDPKAIKKWGTDNSAQWLKDILLIINDIEPFDSEKIDEALNNY
                     LKEKNVKAGNFFNTLRICIVGTSKGPHLSTIMQFIGKQETINRIERGISYFE"
     gene            29155..29595
                     /locus_tag="PKI65_03085"
     CDS             29155..29595
                     /locus_tag="PKI65_03085"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOB77459.1"
                     /translation="MKSTILILLMLSISVIGCKKTNNISTDVVKNPASLEQKKNINHA
                     IIEFETLEHNFGDIMEGEKLTYAFKFKNTGKSDLVITSVSSTCGCTVTDFPNVPIKPG
                     EQGNIMITFNSSGKTGKQVKIITVATNAIPSETQLTIKANVKKP"
     gene            29609..29938
                     /gene="yajC"
                     /locus_tag="PKI65_03090"
     CDS             29609..29938
                     /gene="yajC"
                     /locus_tag="PKI65_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOB77460.1"
                     /translation="MNLMNIFLYAQQAGAQSTQGSMWSTLIMILLLILIFWLFFIRPQ
                     SKKNKEMQKFRDSLKKGDRIVTIGGIHGKIVDFDGNTVIIETEGQGKIRVEKSAIQQY
                     EAGSKES"
     gene            30049..30888
                     /locus_tag="PKI65_03095"
     CDS             30049..30888
                     /locus_tag="PKI65_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77461.1"
                     /translation="MKIDINSALFATFATIIIWFFFRLSILHNIKVPINISLELEGKF
                     AIDTNVTGIFRGEGYQFLLNNKLFKDTFRINITDYHKTENQDTITYFVSKNNIIDKIN
                     SNYLNKLSINLIDIAIDSLIFDILNLVEKDLIIQPNYEITLSGNYNLRNIKVYPSKVK
                     VLLKKSEADTINYIPTELFYIDNINKRSDTVPLAKISDKYKLKKPINKVIVNYDIYKY
                     YDTVFSYTYNNRTYDFNIYSNEPVNKNLFNIKVSNDTLFLEPHTNIQNQIIKIDPSYY
                     YLK"
     gene            complement(30986..33601)
                     /locus_tag="PKI65_03100"
     CDS             complement(30986..33601)
                     /locus_tag="PKI65_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB77462.1"
                     /translation="MIKNIKYFIIFFLTMIILVGCSTKKNTFFSRSYHNLTAHYNAYF
                     NGNEALKQAFALIEKQNVDNFNEILKVFTFDNHQQLSSVSPLCERAIEKGMKVIAKHS
                     MNFNGIEYVKWIDDAYLMIGKARLLKQDYPGAHEIFSHIIVYFDKNPEATEALLWDAI
                     TYLVEGKTSQVLNMLAQVEDRVSRGLTNKSIDRLYHIAEADYYIKVHDYNRAINAIDK
                     ALAKKQKKNTEYRLLFIKAQLLNKLNKNDEALATINKLLKKNPPYEMTLYGNILAAQC
                     LENSHNKSENLKKSLNKLIADSKNKEYLDALYFALAKIELNQKNESQAIEYLKLSAQN
                     SSTNLYQKALTYTTLGDLYYNKRLYKESQMYYDSAVQVMPNNFPDYQKHQNRAATLKD
                     LITALNTIALEDSVQKLSKLSEEELLAHVDILIQNYKIEEQRKKQEEQQRLRQEMANA
                     PVFYNTASTSTQWYFYNNNTITAGKNEFKQRWGGRPLEDLWRLKNKTPIIEDYFAEDT
                     TMLASDSIKNLSDPAKREYYLKNIPKTPEDFSNSNKRIEEAIYTAGKIYFTNLKEYDK
                     ALYYFQELNRRFPNNNKADEAFYLCYISAKEIGKNNDMLSCKDSLLRLYPNSFYAHLV
                     LDPEYYKHIASEEDLASEYLLKTFTALQNNQFSEVLALTDTGITKFHDLSVLAKLSFM
                     KSISLANLTKTDTLLSLLPEIIRSYPNTEVASQSEYLLALLNENNPNKNNNNNQNSDK
                     NSAGIINNPYIIPNEDDIHLIIISFDPLKVTANEMKIAISDFNEQSYKSVNFNVNVTF
                     LTDTRMIASIGYFNSKTKALNYYNTIKENQQIKNLINVADGKIFVINSINYPKFFTEK
                     NEENYINFFNENYLK"
BASE COUNT        11638 a         5225 c         4457 g        12433 t
ORIGIN      
        1 gtctcaatat tatcgcacca taaggaattg aaattatgag aggatgacaa ggtgaaacag
       61 ctaacatgtg aatacatgaa aagtaaagga aaaatatttt accactaaat attgccaaac
      121 tgataattca tgactttgta attttttaat tagtaatttt aaaaaaaaat ctatatgagc
      181 aaaataaata atcatcaaga gttggaagtt taccaacttg cttttacaaa tgctatggaa
      241 atttttaatt tatccaataa ttttccacgt actgaattat attctctaac agatcaaata
      301 cggagatctt ctagatccgt ttgtgcgaat atagcagaag cttttcgtaa acgtagatac
      361 agaaaagctt ttatagcaaa actatctgat gctgaaagcg aagctgcgga aactcaagtc
      421 tggttagatt tcgcttttaa ttgcaattat attgatgaaa atattaaaga ttatctacat
      481 ttacaatacg actttattat cggaaaatta gtaaatatga ttaataattc tgataaatgg
      541 tctttataat atgattactt gattaagatt ctaatatacc ctcacctctc ccaatctcaa
      601 tattagatcg gtcttgtata gaaatcatta ttatttacca ttttttttca aaaatttaat
      661 cttatctttg ctaaaaaatg attatgtatg atctaatagt tataggtagt ggacctggtg
      721 gttatgtagc agccattaga gcagctcaat taggtatgca agtgttagtc gtagaaaaag
      781 aaaacttagg tggtacttgc cttaactggg gatgtatacc caccaaagct cttttacgct
      841 cagctcatat ttatcgagaa ataatctcag ctaataatta tggtattaaa attaatgaac
      901 cacctctaat agatatgcca caaatagtaa aaagaagcag agatattgca tcgatgatga
      961 ataaatctat tgattttctt ttcaaaaaaa ataatgtaaa tctagctcga ggtgtagcta
     1021 aattattatc taatcacaac gtagaagtca aagatgtaga tgataatgtc aatatttatg
     1081 aagcaaaaaa tattattata gctacaggta gtagatctag acaaatatca gctttgcctc
     1141 aagatggtaa gtttatcatt aattacaaag acgctctcat tcttgaccat ttgcctaaag
     1201 aaatggtagt agtcggtagt ggagctatag gaatggaatt cgcagatttt tattcttcac
     1261 taggtgtcaa tgtgactatt attgagtttc ttgatagact cttaccttta gaagatcaag
     1321 agattagtaa aactatggag agaattttta gaaagaaaaa aatcaatgtt cttacatcat
     1381 ccgaagtact aaaaagcgaa ataaaaaata aaaaaatatt tctcacagtg cacactccta
     1441 aaggtattca aaatattgaa actgaaatgg ttttgagtgc tgtaggtatt caagctaata
     1501 tagaaaatat tggattagaa gacataggta ttagaacaaa taaaggtaaa atagtggtag
     1561 atgattttta tcgtactaat attgatggct atttcgctat aggtgatgta atagatactc
     1621 cagctctagc tcatttggct tctaaagaag ctatgatttg tgtagataaa attgcaggta
     1681 aacaagtgaa cccgctcgac tataattttt tcccttcatg tatatatact aatcccgaag
     1741 tagctagctt tggttataga gagcacgaag ctataaatga tggttttaat atcaaaattg
     1801 gaaaattccc tttcactgct aatggaaaag ccaaagctaa tggcctatta gacggtatga
     1861 taaaattgat tttcgatgtt tcaactgata gacttatagg ggctcattta ataggggaaa
     1921 ataccactga aatgattgct gagctcgtag tatctcataa tttgaatgct acagctaaag
     1981 atatctatga atctatccat cctcatccct cactttctga atctatcatg gaagcagccg
     2041 cagcagccta taatgaagct attcatatct gattattttg tatatgatct catttcttta
     2101 aattatacca ttaataaacc cctgcaaatt aatttacagg ggtttatttt tttgaaaaaa
     2161 attgtaaact aaaatcttat ttaatcatag attcgatatc ttccatgata ttttctagat
     2221 catcttcatg ctctacttcg tcttcgagta tctcacaaat cagatgataa gtgataggat
     2281 cattattacc aataaattca agcaatttat tataaacttc aatagcacat tgctctcctt
     2341 taatgttttg ttgcaaaatg atttttacat cgggattttc cggtgcatca tatccacaat
     2401 tagtcatttt gtaccaatct tctggtttta ataaaggcgt acctcctaat tgtaaaatcc
     2461 tttcggcgag tttttcagca tgttctaact cttcattagc atgttctacg agctctcctt
     2521 ccacctctgc tctcattgct ccctttacta ctttggcacc aatccaatat tgataatatg
     2581 ctaaccactc atctgcataa gctttgttta acatttctat cagcttatca acatctaatt
     2641 taacaattga tttacctttt gttcccatat tttacctcct gttttatatg tttttttgta
     2701 attattccta ttatattttt tgcaaattta caatattttt atgaataatg attattttat
     2761 tgtttctttt acatcatttt cttacacttt aatattaatt tattcttata tctatattcc
     2821 ataatcatta atctaaatct taatcgaatt atctgaaaaa atatatttaa ttaaaaaaaa
     2881 gtttacataa ttattaattt ataaaaattt ttctagatca aaataattaa tcaaaaattt
     2941 aacccatcat tctactaaaa aataatattg taaatctaac aattgttata aataaaaagc
     3001 atataatttt aataaaaaaa ttgtaacttt gcttaaatat ttattaattt tttataaaaa
     3061 aaattagtat attcctttat ttttataaaa aaatggaaaa aataattatc attaataatg
     3121 gatcacaata tactcaatta attgctcgca agcttagaga atttaatgtg tattgtgaaa
     3181 ttttaccttg gaatcaggca atagaagtaa caaacgatat aaaaggcatc attcttagtg
     3241 gaagccaata ttctataaat gatcctaatg ctcctacgcc caatttagat gattttattg
     3301 gcaaagttcc tattctagca atatgcttcg gtgctcaata cattgctaaa tattatggtg
     3361 gtgaatttat tgaaaattta acctcagaat gtggtaatgc taaactaaaa tatattgata
     3421 atagtagtcc gatttttaaa aatatctatc ctccaaagat tgtttttatc actcatagcg
     3481 atacaatagt gaaaatacct aaacatttta agattatttg ttctactgat aatgaagaaa
     3541 ttgcaggttt tcaaattagt aaaaacaaaa tttatggtct actatttcat cccgagcatc
     3601 ctcaaacaga aataagtgat gatatattta gaaatttttt agctgtttgt aaggtttctt
     3661 atgactggac accaaatatt tttatcgaaa aaaagattaa tgaaataaaa gaaattgtag
     3721 gtaatgatga agtaataata ggtctctcag gcggcgtaga tagtacagtt acagctattc
     3781 tcatacaaaa agccataggt aatagattgc atggtttttt tatagataca ggacttttga
     3841 gaaaaaatga attcgaagat gtattaaata gttataaaaa aatgggcatt aatgtaaaag
     3901 gaatagacgc taaagatatc tttttatccc ggctaaaagg cataatagac cccgaagaaa
     3961 aaagaaaaat aatcggcatg acttttatag aaatttttga aaatgaagca aaaaaaataa
     4021 aaaatgttcg ttggctagct caaggtacta tatatacaga tgtaatagaa tctggatcag
     4081 taaatggttt gtcaaaaacc attaaatcgc atcataatgt gggtggcttg cctgataaat
     4141 taaatttaaa acttttagaa cctatcagaa tgttattcaa agatgaagtt agaaaaatag
     4201 gtagcgtatt atgtatacca ttagatatat taaataggca tccattccct ggtcccggac
     4261 ttgctgtgag gattttagga gaagtaacat atgaaaaggt taagctttta caagatgcag
     4321 atgatattta tatcagtatg cttaaagaaa ataattttta taacaaaatt tggcaagctt
     4381 ttgctgtgct tttacccgta aaaactgtag gcatttcagg agatgaacgt agctacgatt
     4441 atgcaatagc tattcgtgca atcaattcta ttgatggcat gactgcaaat gcttttgatt
     4501 tgccttttga ttttttaaat aaactagcta acgaaattac tagcaaagtt aacggagtta
     4561 ataggatagt ctatgacctt agccccaaac ctcctgctac tattgaatgg gaataaaata
     4621 ttttttgaat ttcaaaaata tacaatacaa attgatgtat aattttatat tttattaaaa
     4681 ttctttttat tttaaattgt taattttgca taagaaaaag tgccattttg tttcaaaaag
     4741 caacggtttc attatgaaaa taaaataata atattacaat tgtggtaaat ttcggtgata
     4801 caaaagtaca gagatatagt agattagaat taagggcaag acaattagta gcaggatata
     4861 ttactggtat acatcgtagc ccttttcatg gattttcagt tgaattttcg gaatacagac
     4921 cttataatcc tggtgaatct acaagattta ttgattggaa attatatgct agaactgaca
     4981 aattatttgt aaaacgattt gaagacgaaa ctaatctaag agctagaatc ataatagatg
     5041 cttctaattc tatgctatac ccaatgaatg aatacgggga agaggaatta agaagcaaat
     5101 atcatatgtc catgtatatg accgccgtat ttttattatt attttatcaa caaagggatg
     5161 cagctggtat atctctggta agtgatgaaa tattagaaca tacgcacgct cgctcttcat
     5221 acactcatca tagatttctt atatcaatat tagataaata tttattacaa atacgacaat
     5281 taccaaaaac tgaaactaaa ctatcaaaaa atattcatga aatagcagaa aggatacaca
     5341 aacattcatt agtgatagtt atcagcgatc tatttgaagc taatttagat acacaaccat
     5401 tgattaatgc tcttcagcat ttaagatata ataataatga ggtaatagta tttcatttgt
     5461 tagacaaacc aatagaatgg aattttgatt taaaaaatcg cccctacctt tttactgata
     5521 tggagactgg agaaaaaata aaaattcatc cgcgtagtta taaagattta tataaagaaa
     5581 aaataaaaga ttatcaccat caattaaaag ttaatctttt acagttaggc atagattata
     5641 atctggttta tctacaagat ggattagaag gtatattgct tccttttttt atgaaaagaa
     5701 tgtgaaataa tatctaagtc aatgagtaga atatttatca attgatgtag ttaattaata
     5761 atagagccgt attttatacg gctctattat taaattttaa tattcccata gatcatgttc
     5821 gaatataaaa atatcatttt ttattctctc actttctaaa agtgcatcta tcccagtagt
     5881 gtaagatgat atatatctat catagacatt agattctttt attatatagc taccaaacat
     5941 tcttttatta aaagcatcat cataagtaag tctttgacca tcattttttc ggttaaaata
     6001 ttcataacgc actaggtatg gtctcaactg agcaaaagat atccaaaata aaggtctagt
     6061 acccttttca gtaataccat caggttcaaa ttctaccata agaggtaaaa tcccaataat
     6121 tctaacatct agaacagacc gatttttatc aaaaatccaa tcttctttta aacgatatct
     6181 agttacagta gaaggatcaa attctgttac gatgacagta tctctttctt cgccagtgaa
     6241 ggggtcataa gttttagtag tctcttccct tcttattatg ttttgaacag tctctttagt
     6301 cataggtact ttgaaatcat cagattctgg gctaaatgga gtaatgccac cttcttgaac
     6361 acctatcata atgacagaca ttagactata ccgtctatta gttggttgta taggataata
     6421 aagaggatga ttgatttttt ctctcatatc aatttctctc catatacgct tcgcatacaa
     6481 aacatctgat tcatgaataa attgatatgg gactggaact ctattatgta aatcatccat
     6541 ttcataagct ccatctgtag gtgcttcaat gacttgccct tgtagagtta tcacacttaa
     6601 aattaaaaat gttactatta aacttaaata tttcatattt ataaattttc ttaacctgta
     6661 attcgcattg atactgtagg caattctctt acagtaccat caggaccttt tgctttgata
     6721 ttttctatat aaaatctaga tcctctcgta gatctttgta taatatctat tacatcttga
     6781 gtaaatctca tcccagatac ttgcttatct aatagatcac ctgcaggagt tctataagta
     6841 aagctatatg aaactatatc aaatttaaga tcaaattcga aattttcaat atcagctatt
     6901 acataaggat ttgcaagaag ctcggtctta gtataattgc cacctgcttt gcctgctacc
     6961 ttagctatag gcgaaggtac tcttttgacc ctaaactcag cactacccat actcatcatt
     7021 ttaccattca tttcagcagt tacattgact acagatttgg tcccttgact tactcttaca
     7081 atgtacttac catttccttt aggaattata gtaccattag taatagatgc tctaactcta
     7141 tcattaggca cgcctggtac agagatcgaa acgggattat ctactccaat ataaaacaca
     7201 ttcattttgt cagctgatac agtagcagta ggagacatta caaaatattc ttctttaaac
     7261 caataagttt gcacagctcc agattgactt ttaatagtaa tagtcccacc aaatttttta
     7321 gctccgggag aagtagcagg tattctaaga ataccacgcc cactatcacc ttctaattta
     7381 gtaacattac ctaaaacttt gagatttaca gtgtctacat catctcctat taaaatttca
     7441 ggattttggc gagtatcata agctgctacg aatatctcag cttcatattg gctaccagca
     7501 actacaatac ttgatttagg aacaactttt acacctactc tatcaaacgt aaaatcttct
     7561 gcactcactg cagaatacaa ttgagctact acatcagctt ccatatttct aacttcaagt
     7621 attagtttat taagtatcac tatatctgca gctaaaatag tatggtaaaa atttgtcatc
     7681 tcccagttta attgttgatc accctctata gtaggataca ttttattttc aatatctaaa
     7741 cctaattgta ctttgtcagc atacttgccc aataattcaa tcatttgctt tctaaaacct
     7801 actaatttat tttttaattc acgcgcttta cctgcacttc catcttgaga actaccaaca
     7861 aaaaaattag ttggagcatc gaaattatct cttcctttta tatctcttag attgagtttg
     7921 cgagcttctt ctacactcac tctctcagta accgcaatta cttgagcctt taaatcttca
     7981 atatatttga caagttcgtc tgcttctttt ttaactatca atgctttttc ataattatct
     8041 tttacttttt cgggagtcat agcataagct ttgtcaaaca ttgcataatt tccctgaact
     8101 ttgtcataaa aaattctatt tgtttcttct atactatcat ttacaataat aaaagcatcg
     8161 agaatatcct tagatacatt taatgctaac atagccgtaa gcactaaata catcatacct
     8221 atcatttttt gtctcggtgt ttcagcacaa ttttttgcac ccatcttcta atctcctttt
     8281 ttatctatta atactcattg cagctaacat attaccataa acagtattca aagactgcaa
     8341 gtttagcgtt aatttttgtg tttgagactt atactcttgt attgatacta acatttcttg
     8401 aacatttgta gtgaaatctt cgacaccttc gccaaaagat tttactttgc taagctgatt
     8461 ttgcatttca tttaattgta cttcataaat agaattaaga gcagcaagat tgttagtaat
     8521 tttttcaaat tgttctctca atttcacatt atcaatacct tgaaaatcta atttagcagt
     8581 agaattaaca agcatctccg aagattcaat atatgtttta gctaattgat ttgcactatt
     8641 agtagcattc tgaatagttt ctgcatatat tttattaact tgtgctgttt cttccatagt
     8701 actagcacta gtctgggtag cttgagataa cttttgaaaa ttagaaacaa tatttttaag
     8761 attatcagca aattctaaat tagcaccaat aactttagct gtagcttctt tggtctgtga
     8821 atatgtttga tttaactctg ctactgtatt ggcagttgta ttcaaattag taacgaaatt
     8881 atcagtagcc gtagtagcgt ctgccaatcc tgataatttt ttagcgtttt cactcagatt
     8941 gcgcatgcct tcagctaagc tctcaatgag ttctggacct atcttggctt cttcaagcat
     9001 cttatcaagt ttttgagtaa cgctatcttt tactacgatt tcacccccag tacctaaagc
     9061 ttctgcagct ttacgagctt ctatcttttt gagcatttcc tcatccatag gttcatctgg
     9121 atgataataa ccccataatt caggataaac taagctccaa tcaggtataa ctggttgctc
     9181 ttcgaaagct gaaaagaaaa aaattactga ttcagtgatc ataccaatac taagcattat
     9241 agtagcccct ggccagtgtg taattttaaa aagagcacct aaaataacta aagaagctcc
     9301 aataccgtac aattttgtca taaagttttt gtacgctcta ctttttacga ttttgtctaa
     9361 ccaacccata acttatttgt tttttgttat ttaatatatt ttttttttaa cgataaacat
     9421 tagatgacat tggaccatca tctttgtgac gaccgagata cgtctgaaca ttgcgaaaac
     9481 ctatatacga tttagcacta tcttgatatt cataatctcg tgtactcacc tgcatataaa
     9541 agcttatatc tttccaacta ccaccacgta ttacttttct tttcaagaca ggtgggtctg
     9601 tatctttagc ttcataggaa taatccatat taagatcatg cgcaaattga tatgcagaag
     9661 gatcaaatgc attattagtc cattctgaca cattacctgc catatcataa agaccataat
     9721 cattgggtga ataatgagct actatcacag tatgcagtcc accatcatct acataattac
     9781 ctcttaatgg cttaaaatta gccaaaaaac atccattata attacgagta taaggacctc
     9841 cccaaggata agggctaagc tgtaaaccac cacgagcagc atattcccat tcagtttctg
     9901 taggcaaacg aaaatcatta acgtaagcac tgccagtttt tgataaataa ctattcaagt
     9961 aattagtacg ccagatagaa aaggcacgtg cttgcttcca attaacacct acgacaggat
    10021 aatgatcata agcaggatgc cagaaataac tttgtgtcat cggctcatta aaggaatagg
    10081 taaaatcatg tatccaacat aaagtatcag gaaaaacatt aataacatct tttttcacat
    10141 atacagaacg atcttgatat ccttgtggtc tattattcca cataggcata gtaacattat
    10201 cagtttgatc gcttgtcaat gggctgtccc aatttttagc agctgcagct cgataatcta
    10261 tccaatagta ttcataattc cacttacgaa catcaaattg ctttttacga aaatatcttt
    10321 catgttcggg cagatacata tcagctaaag cctctcgctc ttcttgccct tcccaattta
    10381 tttttgtttt ccaatttata gcaggcggga atatctcttc tccatattca ttttgcgtta
    10441 ttaaatgaga ttcatctatt tcccctaata tggtatgagc tattgagtcc ctaacccaat
    10501 aaacaaattg cctgtattca ttattagtaa tttcagtttc atccatataa aaagattgta
    10561 cagaaactac tttattagtt tgaatatgat tataaggcac atcttcatca ctctgtccca
    10621 ttataaaaga tcctaaaggt atgaatacca ttccataagg tgtaggttga taccaagcgg
    10681 gtcgatctct cacgcctaat aattgacctt tatcagaagt atcgcatgag ctaacaacaa
    10741 gtgccattac acaaactaat cctaagattt gtagacttgc ttttgattgc tttatatcca
    10801 tttttatcat tattttctta ttttttaaat taaacgtaaa aaatttaatt aaggttacat
    10861 tttataagaa tttaacattt ctatatcctg aacgatatgt tttcttttca attaagaagc
    10921 aataattaat aaatatttcg tgactaccag agcttacttt atttctgcca atagctgatg
    10981 tagtaaaatc ataagaataa ccaaaagaaa taggtgcaag cccttgattt agactattag
    11041 caaatggttg agcaccaata tttatcatta ctgcatcgtg cactctatag ccaagtccac
    11101 cccataccat accttgataa tatacttgta aatttatatc atattgcaca gtagtgaaat
    11161 cagtctttat taaagctgat ggtcttaatt caaattgatc attattaaga gggaatatat
    11221 aaccacctgt taaaaataaa tgagttttta attttaaatt agaagcctct gctaattcag
    11281 ctttagattg taaaagattt acagctgata accccaccca aaattgatta ggctttaata
    11341 aaaatacacc taaatcacca tctatcacca tatctgattg cttgctacgt tgcattaata
    11401 aaggatcatt aggttctaat ggatttaatt tagaaaaatc taaacttttg tctaaaaaat
    11461 gtaaattaaa accaacacca agagtaaaat ctctaatgta aaaattatat gaataaccaa
    11521 ttttcacccc agtattattt tcataaccta atttatcatt ataaacaatc aaatttaatc
    11581 ctccatgcaa tgccttgata taaccatcta cagctaataa cattgttcta ggattaactg
    11641 tataagtctc tccatttacc acttccttga aacctaccca ttgattgcga tataatgcag
    11701 tagcacaaat agaatttttt accccagcaa atcctggatt atatgcatta aagttttgaa
    11761 aataatgcgt aaattgggct tcttgttgag cttttaaaac ggtggagctc ataaccatca
    11821 ctaaaaatat tataataaaa tataattgtc ttttcatatt catcaatttt gattgtaaaa
    11881 ttatataaaa cttttgaatt acaaaaattt aattagcaaa attaaaaaaa atattaatgt
    11941 ttacaaaaaa tttatggcaa aaataaataa attttttaat aaacaaaatt ttttatttac
    12001 ataaaaatcg cttctgattt tagattgata atatcaaaaa tcagataaat gtcattatac
    12061 tttattttaa ttttatcaaa taatttattt aaatatttta tcttttttat aataaaaaat
    12121 gcaaatatta tctttcgttt aaataaaaat tagagtgatc tttttttttg ttatttgatt
    12181 tcaattctaa aagcatcaga atatttagga tctggtatga tcacatttaa tgtaaaagtc
    12241 tttgtctaat ttttttacat tatgaaacct acatcagcac tatattcgta tcattattac
    12301 tagatataat tttttatgag taaaatatat atattctctg atttattaaa tagtactcta
    12361 ctgtattgta actttatcat ttatattatg agctgataaa gcactaataa atgataaaat
    12421 aaataaaaac acaatgattt tttcattatt ttcattagat aagtatttat ttacaaatat
    12481 ttctaaacat cttctttact ttctaattaa tttcttatgc ccgttctcaa tttgtctcac
    12541 tctgtctcac tctctcccct actctactat aaaaaatcag atacaaatat gaaactatca
    12601 aaatactata tgcatattgc atatcaattt tatcacagag ccaaccaaag aatggaccta
    12661 aaatagcacc gcctattata cccataatca gaagcgcaga aaccatttct gccttctcat
    12721 cggcattacg tagagctata ggccatatac aaggccacat aatagaatga gcaaaaccca
    12781 ataagggcac tgtataaaca gataagtgag ttggcaataa caaaatagct atagtaataa
    12841 ttatccctat aatgctaaaa atcttcaaca ttttttcctg agatatatat tttggaataa
    12901 gtattatacc tagtaaataa cctattatca tacctgtcat cacataagaa gtgaaatagt
    12961 gaggagtgct aaaatctatg cagaagttgc caatgcacaa tagtaacggt tgtaaatttg
    13021 tactttgagc gtatgctaca ctgctatcac cagctattac ttcagcgccg acatataaaa
    13081 aaatagctac aaaaccgagc caagaatgtt tgggaatgcc tttaaacgaa tatttatgct
    13141 tttgtgtctt agctcgtgtc tccacaggct tcatagcata aaaaataaac atcaatgcta
    13201 ttaataaaat agacatgata agatatggcg atatgataga atgtgccaac tgatctaagt
    13261 aattaatgcg ctctgatcca cctaattggc ttaaaaaatt ctgtatttct tgaggtttag
    13321 tatctccgaa taagactcca cctaatatca agccacctaa tgcaccagca attttattag
    13381 ctatacccat taaacttatt cttttagcgg cactatcaat agaacctaac aaagttacat
    13441 agagattaga tgcggtttgc atcaaagtta atccagctcc ttgtaaaaat aaacccagta
    13501 aaaatatagg aaaagatcta gataaagcag caggaataaa tactaatgtt cctatcacta
    13561 tcaatcctaa tcctaataat atacctttaa ttaatgatgt tttttctaat aaaatagccg
    13621 aagggatagc aagtagaaaa tatgaaatat aaaatgcagt agttactaaa aaagcttcaa
    13681 accaccctat ctcacaaatt atgcgaaaat aaggtattaa aatgctattt aaccatgtga
    13741 aaaatccaaa tacaaaaaat aaaataccta tactcagcaa tgaacgacgt tcacttactc
    13801 tatctaagct cttatacata tctgtaaatg atttattttt ttaataatta gcaaatttat
    13861 tactattttt ttaatatatc attgtcattg ctttcaaaaa attataataa tagtatctct
    13921 tttgtttgaa tgatctaaac tgctaacttt taaggtttat atgatcataa ggttctataa
    13981 cgtttattgt gggtaaaaac attttagatt attacaaaaa ataaccattt tttaattatt
    14041 taggctaatt tttttaccac aaagggcacc aagttattac acaaagttca caaagtgcca
    14101 tttataaccc ttttaattcc atctttcttt gtgttcttgg tgccttcttt gagaactttg
    14161 tggttaatat ttaactgtcc aataataaac aaaccaatat taacaaattt atttttgtaa
    14221 aattagtttt tttttaaatc tttacccata taaaacgtta tagaaccgat tataatattg
    14281 tttgtataaa aaataaatta cgagatttta ttttttttaa tcccaatcaa aggtaaattt
    14341 tttcttaggt ttttcaattg tttttgtctt tgtttcatta atagtatctg caggtatcca
    14401 atcatattta aaggtttttg ctttttcatc acctggctta ataatgaaaa gcgaagtatc
    14461 tttttcgaat aaatgcatct cttctttaat attagttttt atactttgct gttctatttt
    14521 aaatgtttct tctctacttt gcttcctttg ttctttatca aaaagtgaat acttataatt
    14581 acctggctta ccatgtatac gtacatataa ggtcatccct ctatcatcat cataatttat
    14641 ataaccataa tcatctatgt aatttatttg agtagctgag tgatggagaa gctcgcgagc
    14701 tttcactgct aaatgataat ctatctcatt atcaagagta tgactaccac taactttaat
    14761 attaatcaca ttactcaaaa tatacatctg gggaatttct attatttgat ctgataaata
    14821 aaaagtattt ttaagttctc caaattttat ataagaaaaa tctcttttta taaatttatt
    14881 taatccaatt agtggttcga aatttatcaa ttctccattt ttaatattaa cattagcggt
    14941 agcttttata gaaggcttta tcagcttcca atcttttgta gtagatccac gtgcctgcaa
    15001 atcaatgtcg gcgatacctt taatattttt atttgttact agggtttgat tgaagttttc
    15061 catttctctg aaaagctgat taatatttat ttgcttagct atgagatcta ttacaaattc
    15121 attttgttta tcactcaaaa ccacatatcc actaccattt atattgcctg agaatgcctt
    15181 aaaatctata ttatctatta taaatttatc actgatcatt ctaccattga tttttaaatc
    15241 accagcagtg aagagcttat gtcttattag atcagagttt atattgattt ttacatgttt
    15301 gcttctactt tctatttcag aatttatttt tacatttttt gctacccaag ttttatataa
    15361 aaaattattt atgttaatat cagcatttgc taatattgtt ggttttacct ttgatttgtc
    15421 ttttgtagtt ttagcttttt gtggagatga agtatttatt aagctatcta ataccatgct
    15481 aggaatattt gctttcaaat ttattatcaa ttgtggctgt gtagagagta gtaaattttt
    15541 ccattttact ataatagcat tagcattttg tatctgctga tgattgatat tacaattgag
    15601 aggatttaat attaaactat catcattgat tataattgtt gcattaacat tattaaaatt
    15661 atatgaagaa ctgaaaaaat caaccgtttg caagtcaaat ttagcattca aatgagcgag
    15721 tgctaaaata cttttatacc ctacattcca atttggtata ttagcactac catttatttt
    15781 atattgcaca gaacctttaa ttaaattaat agattctgaa ttgataaatt tcaactcttc
    15841 tacgttcaaa ataccattta cagctatatc tatctgtgga gctatgagat tatatatttt
    15901 agctttacct tccagattac tatttctacc atatttggct tttaaattat ttatttctac
    15961 gttataaact gaaagatcaa agagtttttt actatttata ttggcaataa gattaatatt
    16021 agaaaaatta aaattttcat ttgtgatttc agcattattt agtgttatta aagcatctaa
    16081 tttgggattt ttttctttag ccaaagaacc ttttatctga gctgataagc ctagcgtacc
    16141 ttttagttta aatttattta aattttcttg aaaattttca ggtaatattg taattatatt
    16201 actcaataaa gtttttttaa tattaatatc agaatgtata gttgggtttt ttttattaaa
    16261 atttaagttt aaagctaaca aaagatcaat accatttata ttagcagaaa tcttatttgc
    16321 attaaatatg gaatttttga aatctattga actattagtt gataaattaa tattgatagg
    16381 ttgcctgata caaaaatcag aacctattat taagctgtct agcacaagtt ttatcgaaga
    16441 ggtttcattg aatacctctt ttttatattc accctttgta agcattttat taatagtggc
    16501 accgatataa aattgatctt ttagtgagtg atatgataaa tgaatatcat taagagaaat
    16561 attaggaaat tctaaaagta tcgggcttgc cttggtagta gtgtctttaa cgaaaatatc
    16621 aaaattatta ctaccatctt ccattacttt taaagatata tttccttgat acagatatat
    16681 ttttttaata ttgtatttgc ctctgataat atcccaaata tttatactaa gatctaaata
    16741 agataatgat agtaattcct tttcacatat ttctacttca gtaaatctaa ctgatacttg
    16801 tggaaaatgg gaaaataaat ttaactttat agtattcaca tgtaatggca ctttgatatg
    16861 tttatttaca tattctacgg ctttatcttt tattatatct tgatgtgtgt atacatatat
    16921 gaatgatgaa ataaaaaaca aaataatgac aataataact atcagtaaaa cttttaataa
    16981 ttttttgagc cagtaatttt ggtccttttc agttgttaat tgcttatcat tcataaaagc
    17041 aaatttacaa tgaaaaatat taattcaaaa attatttttt taaaaaaaac tcttaatata
    17101 ttttattaca caaaatcatt tcaataaatt gcagttaatt ttttgcatat ttctcaaata
    17161 attataattt tgaaaaaata aaaaaatata taatatggat ttttcagatt tgctctttgg
    17221 tttaacaaga ggttttggcc tatttattaa tttattaata ttggcattgt atattatatc
    17281 tctatggata atatttcaaa aagcaggcaa accaggttgg agtgccataa tacctattta
    17341 taatattatt gtttatttag aaataattaa aaaaccgtgg tggtggatat ttttattttt
    17401 aataccagta gtaaatttag tattttatgt aatagtaaat catcaactag ctatatactt
    17461 tggtaaagac gctggattta cagttggttt aatatttttg ccatttattt tttatcctat
    17521 tttagcattt agtaacgctc aatacaataa ttctgacaat taaaaatagt gatttagtaa
    17581 atacagaaaa tgatttaaac aaaaaattaa atttcgatct acttctaacc atttttcctt
    17641 tttttttaaa atacttctaa acattactag atgatagagt ctatttgtat gatcaaaata
    17701 atcaatataa caaatacaaa aaaaaagagg attcaaatat ttgaatcctc ttttttttta
    17761 atatataaac aattaatatt acattaattt aaaatctatt ggtaatgtat agtaaactct
    17821 cacaggttgt cctctttgtt taccaggttt ccattttggc atagatttta cgactcttac
    17881 agcttcttca tcacatcctc cccctatacc tcttagtact ttcacatcag agatactacc
    17941 atctttttct acaacaaatt gcacgtatac tgtaccggta ataccactag ttctagctac
    18001 ttctggatat ctaatattat ttttaatata ttcataaagt gctttttcac caccaggaaa
    18061 ctcagggtac tcttctacga aagtaaatat ctcttcagct acaggctcag gggcctcttc
    18121 tactaaaaca ggaacataag tttgagccgc ttgatcgggt ctatcttcta tacttatgtc
    18181 gatctcatct tgtattattt catcatcacc taccacctct aatattgtag ttacttgtgg
    18241 tgctttgggt ggctctggtt gctcttgttg ggtgatctga actatttctt gatctatctg
    18301 cactggtcca gtagcagtta aaatatcttc ttgtttttca taagttttcc attcaaaagc
    18361 tacaaatact atagttaatg atataaccaa tcctatttgc aaaaataatg ttcgcttacg
    18421 atttaaatct ttctttggat tttttttggg ttccatatct actccacttt ttattaaaat
    18481 aatacgaaaa aaaagtattt ttgttacatt atttattatt tactgaatag cagatttata
    18541 ttctacagca gataataatt tttccacttc agtaggatca gaaattttta tttttactat
    18601 ccacccatct ccataaggat ccttatttat taattctgga ttagtagtta atgtttgatt
    18661 aaattctact atctctcctg aaataggtaa atagatgtct gtcacagttt taacagcttc
    18721 tattgttccc aatacatcac cttgttttaa agtttcacct tctgtaggta tatctacata
    18781 tactatatca cctaattctg attgtgcaaa atcactgata cctatataag catattctcc
    18841 ctctacccta acccattcat gagttttagc gtactttaaa ttttctggaa tattcatata
    18901 tttacatttt ttaagaattc gtttacaaaa ttaaattaaa gtttttaatt aaacaaattt
    18961 ttaaaataac tcaaacatat taagacatta aatgagaaaa taaataggga tctataacga
    19021 ataaaaataa atgtgaagtt attaaaatat actaattata ctgatttaat ttttctggtt
    19081 actcatttta tttatttttt gatttttatt ttctatacaa gtttcattta aaaaatcttg
    19141 ccagcattta tttttgcata aaccaacata tttttttatt gccaatccat tagaaataat
    19201 taacaaagta ggagtagcag atatttgaaa aatattggac catttttcgt atttattaat
    19261 atcgacaatc acataatcta ttgttaaaga atcaaaaaaa gatctttgca ttttacaata
    19321 aggacaatta gatttagtga ataaaagtaa tgtacaaggg gactttaata aatctagtga
    19381 atcaatatta atattttgac cataaatttg attatgattc aaagttaata taaacaagaa
    19441 tgtgagaaat aataattttt tattatttaa agttaatatc ataaggcatc cgtgtcaata
    19501 taattggatt ctcgatatta tttatcaaat aataaggata aaaagattta agctcttcgg
    19561 ctatcacatc atttacaata gcatcactac tcatcatgtc cataagtttt tgatatagac
    19621 caataggggc tggataggta actattctat attcttctat tttagataat cgaactgctt
    19681 ttttaatggc atcattaata ttacctaaac tatctacaag ctttaatctc acaccgtcta
    19741 ttcctgtcca tattctaccc ataccgattg aatcaatata actgattgat aaacctctac
    19801 cctgatttat acgatttaga aaagtattat aaaaattaga aatttctttt tgtaacattt
    19861 tgatagcata attattaggt cgtttcagag aggatacaaa atcactattg gcattagtac
    19921 ccacgccatc ttgtgatatt cctaattttt tattaagtaa attatctaaa actggatata
    19981 acccaaaaac accaatagaa ccagtgagag tggtaggttc tgcgaaaata taattaccag
    20041 cgctagatag ataataacct ccagaagcag cataagaccc catgctaata acaagaggtt
    20101 tgcgagatct gatattcatt agctcatgcc atatcacatc agaagctaca gcatcaccgc
    20161 caggactatt tatacgtaaa actactgctt ttacattatc atcttcagct aattttcgta
    20221 ataatggaac cattttttca gcggttattt tgccatctag agagcctttc atcattatct
    20281 caccctcagc aaatactatt gctatcttgt ttatagtttt tttatcaatt ttaatatttg
    20341 ctttattttg atcaatatat ttgccaatag tgataggttt tactctatta ccaatatcat
    20401 tttctaccca attattaaat tctatagatg tcatcagtgt atctaccata ccattattat
    20461 aagtatattg agcatttaga gcatatagac tatcagcgat aagattgaga gtagcaatag
    20521 gaatatttcg tgcttgactt acctcagtaa taatagtatt ccatatttga ttagcataat
    20581 gtgtatattg ttctctattt tctgggctca ttttatcttg tatgtatggt tctacagcac
    20641 ccttgtattt accttctctg agtactatca tctctactcc cattttttgt aaaagatttt
    20701 tataaaaatt gatttctaag taaacacctt tccatagaat agaacctata ggactgagag
    20761 caattttatc agaagctgta gctacgtaat aaccagtttg agtataatta ttactataag
    20821 aataaacaaa cttaccactt cgtttgaaag aatctaaagc cattttaata ggtctcaatg
    20881 tagttaaatc tgaattaacc tcagagcaat ccaagattat acctttcact ttatcatctt
    20941 ttattgcata attaatagca tttactacgt cataagagct taattgtttt tgtattttac
    21001 catctccata ataaattata ttatcttttt cacattcttt tattggagtt gctaaagata
    21061 tttttaatac attatactca atagtaggtt ctgatgtttt cttaacagat tttactgata
    21121 aggatatcat gcccattata aaaaagaaaa ggagaataaa aaaaattatt agagtaataa
    21181 gattacctaa taaatttgct aataaatttt tactaaaagt actcatataa taaattttta
    21241 caaaagtata ttattttttt gaatttttaa tatactttaa atagttacaa aagttttgtt
    21301 ctaatattat aaaattatta cttgattata aaattatcac ttgtttttat taattatttg
    21361 ataatcataa aattacaatt atcagatcat tttatacttg agtttttagt ttaatcaact
    21421 catcgatttt ttcttgataa aaatcttttt tttctggatt acgaaatttt aatttttcat
    21481 atatttctat agctttatca aaattttttt gtttggtaaa aatacaagca aaggtttcac
    21541 tcacgaaatc atcatcattt actttaatgt ttgcggtcag gtcatctata ttttctggca
    21601 aatcatcttt aggtggcaaa acaatttttg ggtctttttt gataaattca tctataatat
    21661 tactgtattt gtttatttta ttggttgatt tattagtatt ttgtaaataa ttttttaaaa
    21721 tacaaaaaag caattctcta tttggagaaa gcatagaagt tttatataaa agtttttcat
    21781 aattatcatc atcaatagga gtagataata aagaaaggag attagtaaga taagaaaatg
    21841 agaattgaga ataaatatct ttataattaa gattttgttg aaattcgata attgtatcaa
    21901 ttaataaaat tgtatccata tattaccaat ttataaatgc tctattaaaa atatcatcta
    21961 ttaaatcatc aactatctgc tctaccagca tatcctctac agaagatagg gtaacagatg
    22021 agctaaattg ttgataacgg gagaattgtt gtgaaaaatt ttgtttttca tctattttat
    22081 tatcaaatct aatactcaca gttatactta attgatttaa agctgctaca tcattagctt
    22141 gcaaagcagc aggttgtaca acataattag tgatttcgcc ctcaaatgta agatcaccac
    22201 catcatttac cataatcaga ttagtctgtg attgaatgcg ttcacgaagt ttatctgtca
    22261 atagctggct cagagtggga tttaccagag gtgcattatt tacgatatat ttgacagtaa
    22321 aagttttagc accttcaggt atcgaagctc cagaaaatgt aaatctaaca caagccgtta
    22381 aaagaataat tgaaccgata aaaataaaac tggtattaaa tttattcata cctctaaatt
    22441 atattctttg atttttctat ataaagtacg ttcgctgatg cctaactctt cggcagctaa
    22501 tttgcgcctg ccattatgtt tttttatagc tcttactatg agttcctttt cattatcctg
    22561 cagagaaaga ttttcattac tatcatcatt actatcttct acactatctt ctacaacgta
    22621 cgctactgca tcctcagtct tgtctttaaa tttattgata gaatgctcat ctattctaaa
    22681 atcattgata gactgatatg agtcttgttc tataatctta attggtaagc tggatactga
    22741 catatcattt attttctgag ctgtgtctgc atcgatgggt tgattagata ataaatattt
    22801 gaaaactatt tttttgattt cgtttagatc tcgtctcata tctatcaaat atttatatat
    22861 cagttctcgt tcgctaatgc tgtctttatc agataattca gacaaaagta ttggcagatt
    22921 aagtttctct tcgggtagat atttttttaa tgtttcggca ttaatttttc tatttttttc
    22981 aattatagtc atttgttcgg ctacattttt taattgtctc acattacctg gccatttata
    23041 attaataagc atctcaagag catcatcagt taacgtaata ggtggcacat tatacttctc
    23101 ggcaatatca gtagagaatt tttgaaacaa aagtgggata tcctcttttc tttctctaag
    23161 tggcttaata tatataggaa tagtactgag ccggtaaaaa aggtcttcgc gaaattttcc
    23221 tttagcaatg gcttcttgta gattagcatt agtagctgcc actacacgca catctgtttt
    23281 cataactttg ctggaaccta cacgcataaa ttcacctgtt tccagtacac gtaataagcg
    23341 tacctgagta gataaaggca aatcacctac ctcatctaaa aaaatagtcc ccccgttagc
    23401 tacctcaaaa taacctttac gagaatctat ggcgccagtg aaagatccct tttcatgccc
    23461 aaaaagttca ctgtctatgg tcccctctgg tattgcacca caattaacag ctatatatgg
    23521 accgtgtttg cgtgcactat atgtatgaat aatttttgga aaaaattctt tacccgttcc
    23581 actctctcca ataatcaata ctgtcaaatc tgtaggagct acttgtatag caacttctat
    23641 agcatgattt agtaaaggcg aattgcctat aatgccaaat ctttgtttta tcaagtatag
    23701 ctcatccatt gttttttata caaatataat aaatttttta ataacaataa attaaaaaaa
    23761 tattttttgt tttttaatta aaaaaaataa ctaaatttgc agtataatgt tttaaaaaaa
    23821 tagctatgaa aaagaataat ttaatcattg tggcagttat tacaactttt ttaatttcaa
    23881 caagttgtag taaatatgat gagggacctc ttatcagttt tagatctaaa actgatagat
    23941 tgtgtcaaac atggaaacct aaaaagattt taataaacgg tgcagagact atattgacac
    24001 ctgatcagca aaaaactact attgagttta aaaaagatgg tgatctgtat ctaactacct
    24061 atagattgtc aatagctttc ccacttacag gtacttggaa attttatgat aaagatgaaa
    24121 aattaatact atctatagat aagggtgatc cagaacctca agtagacaca gtgaagatat
    24181 taagattaat gaaagatgag ctatggttaa agcaaaaaaa ggataatata atagagattc
    24241 acttagaacc aaaataaatc acataggaga tatattaatt ttttttgatt ccaggtaaca
    24301 ttggtacaaa tgtaaagaat ccatgttttt cttctttgaa attattttca tcttccttta
    24361 ataatcttgt cattacttgg gtaccaagag tataactcac aggggctacg ataataccac
    24421 ctatttttaa ctgatctttt agactatctg gaatttgatc tactgcagca gtgataataa
    24481 ttctatcata tggtgcatat tgaggtaagc cttcatatcc atcacctaaa aagcattgaa
    24541 tattattata accgagagca gataatatat cttttgcatt taaatgcaag tttagaatac
    24601 gttcaattgt aaaaacttga gcacccattt gagcgagcac agcagcctga taaccactgc
    24661 cagtacctat ttctaaaact ttcatatcag gcttgacatc taaaagttca gtttgaaatg
    24721 caactgtata tggctggcta atagtctgtc cctcgcctat atcaaagggt ttatctagat
    24781 atgcgaaatg ttcgagaccc gcaggcaaaa aaaagtgcct gggtatttct ctcatagctt
    24841 tcaatacagc ttcatcatta atccctttac gttgtatttc attgattagc ttatttcgca
    24901 ttccttgagt ttctaatgta tcttttaaaa tcctattgcc taatttaatt attcgttctt
    24961 tcatagttaa taaatttaat cactataagg tatttcaatt aataatttta agattttttt
    25021 tactacttaa tagataaaat acaaaataaa gaaaaaaatt tgaatttcga gtttcaaatt
    25081 atttagtttt ttataaactt ttaattgtca ttcactataa aatgaaatag aatatatttg
    25141 tatttggcgg agagagaggg attcgaaccc ccggtccgtc gaaaaacgga caacggtttt
    25201 caagaccgcc gcattcaacc gctctgccat ctctccaatt gcaaaataat aaagaaattt
    25261 ttatataaac aaatttttat tatccactta tataattaat ttcaaatatc gagcacactc
    25321 atacattaca ttaatcacta ttgaaaaaaa gagaaattta ctttaccata tgctctatga
    25381 tctatcaatc gtggatgatt ttgaaatgaa attttagcac tatgttctat tattaacaaa
    25441 ccttcgtcgt ttaataattt attatcaaag atcaaatcag gtattttttc gatattttct
    25501 atgttatagg gaggatcagc gaaaatgata tcaaacctat catagctatt agcaatgtat
    25561 tgaaaagcat ctacctgata aacactgata gactgtaatt taattttttc gatattttgt
    25621 aaaataaatt tataccaaaa ttcatctttt tctattgcta taacattttt agcaccacgt
    25681 gaagcaaatt catatgataa attacccgta ccactgaaaa gatctaaaac atctatgtct
    25741 ataaaatcgg tataatatcg taaaatatta aataaagctt ctttagcaaa atcagttgta
    25801 ggactgatat cgattttgct gggtagagtt atttgtcttt ttttgtgaaa accacctata
    25861 attcgcataa tttaattgaa gaaataaaaa aatgtgtgaa ttctcctaaa atatttgtag
    25921 aatgagataa taattttatc tctgaaaaaa aaggatctac cttttctatc aagtgcgaat
    25981 cttcagtaat agcacctgaa aaatacatag gtaatgaggt atctgagagc cctacgacat
    26041 taatagcact actaacataa taaagcatat ccatcggagt tttatattct aataaattat
    26101 aaaacaataa tttaccttca caaactatag ctataatcat agtattagta tgaatatcaa
    26161 taaaaacacc atcttcattt ttatctagaa tatattgaat ccataaaaat tcgtgtggag
    26221 tgtatggata aatattgatt tggtcaggtg tattgatttt tttattgagc attatagcat
    26281 ttagagcagg tatagagttc attttaatat cgtcagtatt ttcccatcct aatatagtag
    26341 caaaatcatt tttagaatat tttaataaat attcatcagg taatatagta ctttgtgttg
    26401 atgatattat aaggttaaat ttagttatat tatttagttc ttttaataaa ttatcatcaa
    26461 atgtgtccca tggaagcgaa tttattttaa ttggtttttt atcattcaaa taaatagcat
    26521 aagccccttc tgcaaagagg cttaatacta tattattaat ttctaattct gaataaaata
    26581 ttttttcaaa attctttaaa ctcattactc ccaattacca tctgttatag gttcagttat
    26641 agaaccaaat tttagaaagc cctcagcatc tatgccatat ttttctaaat cataatcttt
    26701 cataaaagat ttgtaaggtg ctttcacttc aatagtattt acccaaacat tacccctatt
    26761 tattctatct gttctaatga taaaagtatc tttaggagta ccagtaatat ctgaatatgg
    26821 tatatatttt aaattttttg gattgaaatt tggaatattt ttaaataatg aatcttttac
    26881 gattttatat cctatagtat ctctaataaa aactgagaag gtagtatctt taggatcagc
    26941 agttaatttt acaataggta ttttaccatt ttcaataaaa tctgctaaag aatcccaatt
    27001 tgcagtgtat ctgttattta cttgtaaata catttgctca gcagagcgta tttgcttcaa
    27061 tttttctatt actttttctt gtcttttatt aaattctctt tgaaaattta atggatccga
    27121 aatactcttt acaatcatta tagcaagtac tattattact acccatagta ctatctgcac
    27181 aattaactta gtggttttca tattttttaa tgatttttcg caaaattaaa aaagaaatta
    27241 ctaataaaaa aattttttat tagtattatc atctaaacct tagatatctt tacgatttat
    27301 tatgattttt aatagtatta gatcctttga tacattaatc acgattatag aggcttatct
    27361 atatatattt cttaatatat ctaaaccagc attattcaaa ataactaatt cctctctcga
    27421 ttctatttat ggtttcttgt ttaccaataa actgcataat ggtagataaa tgaggacctt
    27481 tgctagtgcc tactatacag atacgtaagg tattaaaaaa attaccagct ttaacatttt
    27541 tttcttttag ataattatta agagcttcat cgattttttc tgaatcaaaa ggttctatat
    27601 cgttgattat caataatata tctttgagcc attgagcact attatctgtt ccccattttt
    27661 ttattgcttt tggatctaat gtttctgggg ttttaaagaa aaaaatggct tcggttacaa
    27721 gatcctttac gaaatctacc ctttctcgca taagttcagc gacaaataca gcttcttcta
    27781 atgttacatt tatatttact tttgataatt catctttaaa ttcttgagct actaatttga
    27841 tatctctttt ttgtagataa acgtgattaa accatctcgc tttgatagga tcaaatctag
    27901 caccagattt atgcacatct tttagatcaa aagcttctat catttcctcc atagacatta
    27961 tttctctatc atcaccagga ttccacccca atagagccag catattaacg aaagcttcgg
    28021 aataataacc atcttcgcga aagccacgat agatttcttt tgttttagga tcttgccatg
    28081 agatagggta tacactgaaa cctaatctat caccatctct tttactcaat ttacctttac
    28141 catctggcct taatatcaaa ggcaaatgag caaactccgg cacctcccaa tcaaaagctt
    28201 gataaatcaa tacatgtaat ggcaaactgg gcaaccattc ttctccacgt atcacatgcg
    28261 atattttcat taaatgatca tctactatat ttgctaaatg ataagtaggc ataccatcac
    28321 ttttgaaaag tactttatca tctattataa tagagtttac ttttatctca tcacggatta
    28381 gatcatttac agtcacttct ttagcttcag gtactttgaa tctgatgaca tgggggtggc
    28441 tggtatctaa caattttttc acttctgatg ctggtagact aagtgaattt tttaaattca
    28501 ttctattatt tattccatat tgaaaagttt cgcctctgct ttcatattct ttacgaattt
    28561 gatttaactc atcaggtgtt tcgaaagcat aataagctgc tcctttttgt attaaaatat
    28621 ttatatattc tctataaata ccagttctat cactctgtct atatgggcca tattcgcctc
    28681 cgatattaat gccttcatca aattgaatac cgcaccaagc taatgactct aatatatatt
    28741 cttctgcacc agatacgtat ctagactgat ctgtatcctc tattctcaaa ataaatttac
    28801 ctttattttt gcgtgcaaaa aaataattgt acaaagctgt cctaacacct ccaatatgta
    28861 atggaccagt aggactaggt gcgaaacgaa ctctaaccat tttttattgc gaaattaata
    28921 gttttttttt aaaaaaaatt acaattctaa gcaatatttt atataataat gcttagacgc
    28981 aaacttttta attcatttag tatgttactt ttgataaaag ttaatatcga tttacaacct
    29041 gagaacaatg tatttttttt tatacttaca ttaattttca tttttataga aataaattaa
    29101 gattttttta ttttataaat ttcttttact tttgtaaaaa atttttataa aaaaatgaaa
    29161 tccaccattt tgattttatt aatgctaagc ataagtgtaa taggatgcaa aaaaactaac
    29221 aacatatcaa ctgatgtagt aaaaaatcca gctagcttag aacaaaagaa aaatataaat
    29281 catgctataa tagaattcga aactttagaa cataactttg gtgatatcat ggaaggcgaa
    29341 aaacttactt atgcttttaa atttaaaaat actggtaaat ctgatttggt aattacctca
    29401 gtgtctagca catgcggatg tacagttact gattttccta atgttcctat taaacctggt
    29461 gagcagggca atataatgat aactttcaat tcgagtggca aaactggcaa gcaagttaaa
    29521 ataattactg tagctactaa tgctattcct agtgaaactc aattgacaat aaaagcaaac
    29581 gttaaaaaac cataaaaaat aaataaatat gaatttaatg aacattttcc tttatgcaca
    29641 acaagctgga gctcaaagta cccaaggtag tatgtggtca actttgataa tgattctcct
    29701 tttgatacta atcttttggc tgttttttat acgtcctcaa tcaaaaaaga acaaagagat
    29761 gcaaaaattt agagatagcc taaaaaaagg agatcgtata gttactattg gaggtattca
    29821 tggtaaaatc gttgatttcg acggtaatac tgtaataata gaaactgaag gacaaggaaa
    29881 aataagagta gaaaaatctg ctattcaaca atatgaagcg ggaagtaaag aatcctaatg
    29941 ctactcaatg ctttcttggt ttttaaataa tttttccttc actactattt attgaattat
    30001 tttaattagt aaaatagtat caaagtgtat gtagtaagtt gatttgttat gaaaattgat
    30061 attaatagtg ctttatttgc tacatttgct accattatta tttggttttt ttttagatta
    30121 tctatcctgc ataatattaa agtacctatt aatatatctt tagaattaga aggcaaattt
    30181 gcaatagata ctaatgttac tggtatattt agaggtgaag gatatcaatt tcttcttaat
    30241 aataagcttt tcaaagatac ttttcgtata aatattactg attatcataa gaccgaaaat
    30301 caagatacta ttacttattt cgtttcaaaa aataatatta ttgataagat taattctaat
    30361 tatttgaata aactatctat caatttgata gatatagcta tcgatagttt gatttttgat
    30421 atattaaact tagtagaaaa agatttaata atacaaccaa attatgaaat tactctatcg
    30481 ggaaattaca atttgagaaa cataaaagtt tatccttcta aagtaaaagt tttattaaaa
    30541 aaatcagaag ctgataccat taattatatt cctacagaat tattctacat agacaatatt
    30601 aataaaagat ctgatactgt acctttggct aaaatatctg ataaatacaa actgaaaaaa
    30661 cctattaata aagttatagt caattacgat atttataaat attacgatac agtattttca
    30721 tatacataca ataaccgtac atatgatttt aatatttatt ctaatgaacc tgtaaataaa
    30781 aacttattta atattaaagt aagcaatgac actttatttt tagagccaca tactaatatt
    30841 caaaatcaaa ttatcaaaat cgatccctca tattattatc taaagtgaat ttattaaatt
    30901 attaataaaa acgatcaaat ataacgagag tatatgagaa gtggagtgga tgctaaatga
    30961 aatatcgcaa attatatgta acgagctact tcaaataatt ttcattaaag aaattgatat
    31021 agttttcttc atttttttct gtaaaaaatt taggataatt gatcgaattt attacaaaaa
    31081 ttttaccatc agctacatta attaaatttt taatttgttg attttctttt atagtattat
    31141 aataattaag agccttagtt ttagagttaa aatatccaat agatgctatc atgcgagtat
    31201 ctgttaaaaa agtaacattt acattaaagt ttactgattt ataagattgc tcattaaaat
    31261 cagaaatagc tattttcatt tcattagcag tgactttcaa tggatcgaaa gaaataatta
    31321 tcagatgtat gtcatcttca ttaggtatga tatagggatt attgataata ccagctgagt
    31381 ttttatcact attttggtta ttattattat ttttatttgg attattttca tttaataaag
    31441 caagtaaata ttctgattga gaagctacct cagtattggg ataagatctg ataatctctg
    31501 gaagcaaaga caataaagta tctgttttag taagattagc gagagatatt gatttcataa
    31561 acgatagctt agcgagcact gataaatcat gaaattttgt tataccagta tctgttagag
    31621 ctagcacttc agagaattga ttgttttgta aagctgtaaa tgtttttaat aaatattcac
    31681 tagctagatc ctcctctgaa gctatatgtt tataatattc gggatctaag accagatgtg
    31741 cgtaaaagga attgggatat aaacgcaaaa gactgtcttt acaagacaac atatcattat
    31801 ttttacctat ctccttagct gatatatagc agagataaaa agcttcatcg gctttattat
    31861 tgttaggaaa acgacgatta agctcttgaa aataatacaa ggctttgtca tattctttta
    31921 gattagtgaa atagatttta ccagcagtat atatagcttc ttcgattctt ttattagaat
    31981 tagaaaaatc ttctggtgtt ttaggaatat tctttaaata atattctcgt ttagcgggat
    32041 ctgaaagatt tttaattgaa tcagaagcta gcatagtagt atcttctgcg aaataatctt
    32101 ctattatagg agttttattt tttaacctcc atagatcttc taatggtcta ccaccccatc
    32161 tttgcttaaa ttcatttttg cctgcagtta tagtattatt attataaaaa taccattgag
    32221 tactagtgga tgcagtattg taaaatacag gagcattagc catctcttgt cgcagtcttt
    32281 gctgctcttc ttgttttttt ctttgctctt ctattttata attttgtatt aatatatcta
    32341 cgtgggccaa aagttcctct tctgataatt tagaaagttt ttgtactgaa tcttctaatg
    32401 caatagtatt gagagctgta atgagatctt tgagagtagc agctctattt tgatgttttt
    32461 gataatcagg aaaattatta ggcattactt gcactgccga atcataatac atttgagatt
    32521 ctttatacaa acgcttatta taatataaat cccccaaagt agtatatgtc agtgcttttt
    32581 gatataaatt agtagagcta ttctgagctg ataatttcaa atattctatt gcttggcttt
    32641 catttttttg atttaattct attttagcga gtgcgaagta taaggcatcg agatattctt
    32701 tgtttttgct atctgctatc aatttattca atgatttctt taaattttct gatttattat
    32761 gtgaattttc taaacattgt gctgctagta tattaccata aagagtcatc tcatagggag
    32821 gatttttttt gagaagttta ttaatagtag cgagagcttc gtcattttta tttaatttat
    32881 ttagtaattg agcttttata aataataatc tatattctgt attctttttt tgcttttttg
    32941 ctagagcttt gtcaatagca ttgattgctc tattataatc atgaactttg atataataat
    33001 cagcctcagc gatatgatat aatctatcta tagatttatt agtgagtcca cgacttacac
    33061 gatcttctac ttgggcaagc atatttaata cttgactagt tttgccttct acgagatacg
    33121 taatagcatc ccataataat gcttctgtag cttcaggatt tttatcaaaa tatacaatta
    33181 tatgagaaaa aatttcatga gcacctgggt agtcttgctt taatagccgc gctttgccta
    33241 tcattaaata tgcatcatct atccacttaa catattctat gccattaaaa ttcatactat
    33301 gcttagctat cactttcatt cccttttcta ttgctctttc acataaaggt gatactgatg
    33361 ataactgttg atgattatca aaagtgaata cttttaatat ttcattaaaa ttgtctacat
    33421 tttgcttctc tatgagtgca aaagcttgct tcagtgcttc attaccatta aaatatgcat
    33481 tgtaatgagc tgtaagatta tgatatgaac gtgaaaaaaa tgtatttttc ttagtagagc
    33541 atcctactaa aattatcatt gtcaagaaaa atattataaa atattttatg ttttttatca
    33601 ttaatactaa aaaatttttt tgaataacgt atattaaaca aaattaatat attttaataa
    33661 tttacaaaat tattaatttt ttttggttct ataacgtttt acatgtataa agattaaaaa
    33721 gagttgattt ttaatattgg tttatttatt att
//
LOCUS       DAOPRN010000016        33317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1032_length_33317_cov_108.396488, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000016 DAOPRN010000000
VERSION     DAOPRN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33317
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1032_length_33317_cov_108.396488"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..378
                     /locus_tag="PKI65_03105"
     CDS             196..378
                     /locus_tag="PKI65_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77463.1"
                     /translation="MRRFRGVVTKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
     gene            546..899
                     /locus_tag="PKI65_03110"
     CDS             546..899
                     /locus_tag="PKI65_03110"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOB77464.1"
                     /translation="MFIEILKSKIHRAFVTEVNINYQGSITIDEDLMDAANIIENEKV
                     QVLNYNNGVRIDTYVIKGKRGSGEICINGPAARNFIIEDPIIVLSFATIDCEEAKKIT
                     PKIIYVDQSNKIISK"
     gene            896..1897
                     /locus_tag="PKI65_03115"
     CDS             896..1897
                     /locus_tag="PKI65_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOB77465.1"
                     /translation="MKPKVKTYLQTFIFLAIGIFLIFWFLNKLSPAEKQEIWHYFLQA
                     NPLWMIIAMFIGIISHIFRALRWNLLIESVTKKPKIKNTFWAVMSGYLVNYAVPRLGE
                     ITRCALLGKKEKIAVDTVLGTMISERLFDVICYVIIFILAFVALTAKVIAFLDNYNKP
                     TFISWQFALILLLAAILIVVAYKLWRKYKPNSKLGQKISDSIHRFIDGGKSIINLKKK
                     GLFIVYSILIWVCYLLMTYFAFLTIDATYHLPIDAAFAVLALGTIGMIVIQGGIGVYP
                     IIVSQVLILYGVDKVGGYALGWISWIIQTVIVLILGLIGFIILSKKNKYEQIPDFNK"
     gene            1869..2372
                     /gene="rfaE2"
                     /locus_tag="PKI65_03120"
     CDS             1869..2372
                     /gene="rfaE2"
                     /locus_tag="PKI65_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOB77466.1"
                     /translation="MNKYQILINKILTLNDLLLKIKNWRDNGDIIVFTNGCFDLIHVG
                     HIEYLTKAADMGDRLIIGLNTDESVKRLKGPDRPILDENARAKTLSALFFVDVVTLFN
                     EDTPYELIDAIKPDILVKGGDYEIDEIIGADIVKSYDGKVEIIPLVPGYSTTNIINKI
                     NKLYDNN"
     gene            2419..3375
                     /locus_tag="PKI65_03125"
     CDS             2419..3375
                     /locus_tag="PKI65_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOB77467.1"
                     /translation="MINQTILFLDSTHEYLPQQFINNGYEVIMHYDLPEKLNFDIRKK
                     VYGIIIRSRLILDKDKIDLFPNLHFIARAGSGMENINIEYAKSKNIYCINSPEGNRIA
                     VAEHAIGMLLSLMNNIIKANLEIKNGLWRREANRGYELDGKTFGIIGYGNVGTAIAER
                     LQGFNMNIIAYDKYKHDFSDQTVTEVSLDELLESSDFISMHVPLTRETIYMVNDDFFS
                     KLSKSIWFINTSRGQIVNTKSLLNAIDSGIVLGACLDVLEWENHSFENIENMLSHPDD
                     ILKRVINHPQILLTPHVAGWTFESNKKHAEILWKKLKNLKLV"
     gene            complement(3455..4048)
                     /locus_tag="PKI65_03130"
     CDS             complement(3455..4048)
                     /locus_tag="PKI65_03130"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOB77468.1"
                     /translation="MINIVLFGPLGSGKGTQAKKIADKYGFIHFSTGELLREEIRKQT
                     KIGKKIKSIVNSGALVPDDIMEEIVENFLKENCNTKGIIFDGFPRTLRQAKMLDEELE
                     KYNLLLDLIIKLDVPEEIIKSRLLYRAQKEGRTDDNEAIISKRLKYYYRDTLPVLDYY
                     KQTNKNIVSICGHRDVDIVFNDICLHIDKILLQKMQI"
     gene            complement(4045..4593)
                     /gene="hpt"
                     /locus_tag="PKI65_03135"
     CDS             complement(4045..4593)
                     /gene="hpt"
                     /locus_tag="PKI65_03135"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOB77469.1"
                     /translation="MKTMRIHDRLFELFITEEQINKRITELSIQIIKDYADKMPVFIS
                     VLNGSFIFTTEIVKRFPYNCEVDFIKVSSYVGISSSGNVSLIANSIKADLNNRYIIIL
                     EDIIDTGLTIKNLLNDLSKYSPKKISIATLLFKPEVFKFNDKIKIDYIGFNIKNEFVV
                     GYGLDYNGLGRNYLDIYKLKEI"
     gene            complement(4680..5105)
                     /locus_tag="PKI65_03140"
     CDS             complement(4680..5105)
                     /locus_tag="PKI65_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77470.1"
                     /translation="MFKKHRLFFLIFLTFLIYACKDDDNYIPYVPVNIRIDVYDALYS
                     DINVVGGYMYITGGYKGIIIYRKSQDEFVTMERACPYKPLLDCERLDVDDSTVLFVKD
                     ECCGSKFLIIDGSIINGPATKPALLYKNNFDGRYLYIYN"
     gene            complement(5109..5456)
                     /locus_tag="PKI65_03145"
     CDS             complement(5109..5456)
                     /locus_tag="PKI65_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77471.1"
                     /translation="MIQISNQKTIIFLIIILFSGLSMTCYSCKSQSTSINSQEKKIEK
                     EKKKRQKDDTILYQKAVKQQMNNQTPETREAMKDAQKQAKRNRENKKEFFIKRWIKNW
                     QYKQQHKPPKTKG"
     gene            complement(5674..7716)
                     /gene="metG"
                     /locus_tag="PKI65_03150"
     CDS             complement(5674..7716)
                     /gene="metG"
                     /locus_tag="PKI65_03150"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOB77472.1"
                     /translation="MAESRNYKKYTVTTALPYANGPIHIGHLAGVYVPADIYVRYLRM
                     RGRDVLFVGGSDEHGVPITIRARQENISPQQIVDHYHNKIKKSFEDFGISFDIYSRTS
                     NPIHYQLSQDFFLKLLNKGELFTQNILQYYDEEAGQFLADRYIIGTCPHCGYTEAYGD
                     QCEKCGSSLSPQELINPRSTLSGSAPVLKETLHWYLPLDKYEGWLREWILEGHKEWKP
                     NVYGQCKSWLDQGLQPRAVTRDLDWGVPVPLPDAEGKVLYVWFEAPLGYISASIDWSK
                     KTGKDWQPYWKNQDTKLIHFIGKDNIVFHCIIFPVMLKMEGSYILPENVPANEFMNLE
                     GKKISTSRNWAVWLDEYLVDFPNKQDVLRYVLTINMPETKDSDFTWKNFQQHNNNELA
                     AIYGNLVNRALVLTHKYYSGLVPENFLIEDEEMIYSFITKKRDEVTNYLEQFKFREAL
                     NSMMDVARFGNKYLTEKEPWKLAKTSPNITKGIIYNILQLVANLSLLSEPFLPFTAQK
                     LRNILHINLLDWDKIGTPLLKTGARIEQPIILFEQISDEVIENQLNKLHKSDAVEVVN
                     RLDDLKPIISWEDFEKIDLRVGQVISAEYISNADKLLKLQINIGGQIRTIVSGIAQHI
                     SPDQIINKKVILVANLEKKKIRGVESEGMVLFVENKNGDLMLVCADDNAKPGDKVK"
     gene            8413..9222
                     /gene="deoC"
                     /locus_tag="PKI65_03155"
     CDS             8413..9222
                     /gene="deoC"
                     /locus_tag="PKI65_03155"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOB77473.1"
                     /translation="MNWEHINEDIDKIIKNASITQKIAKELISFIDLTTLEGTDNNLV
                     VKELIAQAKESYQKTNGKGVAAVCVYPVFADFAANELANTNIHLACVAGSFPSGQSPL
                     HVRIAEVEHAVAHGAQEIDMVISRGAALAGDFDFVKYEIALHKKACGSAHLKVILETG
                     ELKDPDLIYNISMLAMENGADFIKTSTGKTSPAATLDAAFVMCCAIKEYYALTNKKIG
                     FKPAGGIREPQEAFNYYLIVKNILGIDWLTPNLFRIGASKLLLNLYKFSEL"
     gene            9219..10844
                     /gene="pckA"
                     /locus_tag="PKI65_03160"
     CDS             9219..10844
                     /gene="pckA"
                     /locus_tag="PKI65_03160"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR00224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOB77474.1"
                     /translation="MNSDKLFNDLIDLGIKTSKVHYNLSYEELFEHETRPDLKDYAKG
                     FVTKCGAVAVDTGIFTGRSPKDKYVVYDDTTKDVVWWKNEINKTSDNKPMSPTVWTEL
                     KQLVVNYFCDKELYVMDAFVGASEKHRLPIRVITDIAWAAHFVKNMFIVPTEEELKNF
                     EPQFIMLHAPRVTNPNWLEHKLNSEVFVTFNFTDKMALVGGTWYGGEIKKGFFSVMNY
                     YLPLKNVASMHCSANMGKDGDTAIFFGLSGTGKTTLSADPKRFLIGDDEHGWNEEGIF
                     NFEGGCYAKCINLDPKKEPQIYQAIRRNTILENVVYNPETGEIDFSDASKTENTRASY
                     PLSHIPNAIIPSIGPHPKNIIFLTADAFGILPPVAVLSPEEAMYYFISGYTAKLAGTE
                     RGIIEPIPTFSAAFGAAFLTLPPIIYARLLADKMQKHNAHAYLVNTGWIGGPYGIGNR
                     IDIDVSRKIIHAILDGSILQTNYDIMPFFNLKIPKHIEGIDDNILNPRYTWEDKEAYD
                     KQAQTLAKAFVDNFQSFTTDDETKELIDFGPKI"
     gene            complement(11172..12305)
                     /locus_tag="PKI65_03165"
     CDS             complement(11172..12305)
                     /locus_tag="PKI65_03165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOB77475.1"
                     /translation="MKKILVLFLALFSFSILYCQDDKSENIKLIFIGDIMQHTPQINS
                     AWNGKNYDYSENFKYLLPLFNYADFVIGNLETTFNGAPYTGYPAFSAPDDLAGALKRA
                     SVDILVTANNHSLDKGINGLIRTINVLDSLYINHTGTFKNKIDFQKNHPLIIQKDDIR
                     LAILNYTYGTNALLPTSSIIINLIDTNEIKDDLLLAKSYNPDGIIIYFHWGEEYQRLP
                     NNYQKTLAEFCIKNGAHYIIGSHPHVIQPMIKSIIDTVKNLESIVYYSLGNFISNQRW
                     IHTDGGAIAYLEFTKNKDGLQLSQAGYLLSWTWKKLENNRYKYINLPAWKYDKLANLY
                     PEDMSAMKFFLDSTRVHLNKYNVGIQELYFDEVQNVIKSINMK"
     gene            complement(12316..13659)
                     /locus_tag="PKI65_03170"
     CDS             complement(12316..13659)
                     /locus_tag="PKI65_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77476.1"
                     /translation="MNKYLEFQINYPYFNYNTYNIQLLSDKLKISFDFDNGKFSFHPS
                     SYWRFNKTLNAKKLNFNEIEAFVFNIGMIELISYWKATCSPIVNINLSFLSDYQTMWW
                     KNLYYYGMGEFFYKNNIEIKIDEFIHFYINNFKTYKKPNLKYLDDNKVIVPIGGGKDS
                     IVTLELLRRSDFEIIPLIINPNEATLRILDIAHIPRSDIIEIHRMIDPTLLELNKKGF
                     FNGHTPFSAMLAFYSIFSAYMIGAKYIALSNEFSANESTVRDSYVNHQYSKSLKFEND
                     FRDYVKKYLNDEIEYFSFLRPLHELQVVKLFTNYPQYFYAFRSCNIGSKNDSWCANCA
                     KCLFVALMLSVYLDPQEIKKILGVDIFSNLKLKPIMDELLGLKETKPFECVGTIYEVR
                     AVVNHLLMDKNYNCLPLFSDLKPMPAANLDDLEKSWNDIHFLPEKFEKLLKKNFY"
     gene            13725..14321
                     /locus_tag="PKI65_03175"
     CDS             13725..14321
                     /locus_tag="PKI65_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOB77477.1"
                     /translation="MMNKEYKLKIKRNDIEPTIGRVLVAEPTTYDFFFSRSIVLLLEH
                     EDREGSAGVILNKKISDNFKDIYNKSKIKADFPLYLGGPVDTNKLFVLHRLGDIIPKS
                     FEVIPGLWWGGDVKTIYTLVNNKIANNNNLKVFQGYSGWAPMQLREEMKKNYWAVTEL
                     SIDEIFEMPVETMWKKCIENLGPEYEFWQHIPENPTFN"
     gene            15093..15845
                     /locus_tag="PKI65_03180"
     CDS             15093..15845
                     /locus_tag="PKI65_03180"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOB77478.1"
                     /translation="MIEKNIEKAVDVLAKKDNIVAMTGSGISAASGLKTFRGKDGLWE
                     EYNPQALEISYFYEHRAESWEWIYKLFFEPLQYAKPNPAHYALAELEKMNKLNAIITQ
                     NIDGLHQAAGSKNVIEFHGTTRNLICTKCGAEYSREQIDFSNLPPKCNNCGEILKPDF
                     VFFGEMIPHKAAEYAYHHAMHCDAMMIIGTSGIVMPAADLPRVAKQNNAVLIEINLEQ
                     NNSKIFIPDIFIQGDATVILPKIVDKLKQKLL"
     gene            complement(16037..16276)
                     /locus_tag="PKI65_03185"
     CDS             complement(16037..16276)
                     /locus_tag="PKI65_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77479.1"
                     /translation="MSLYVKLINPKEVIFEGVVDTIWLPGYVSPFQVLPNHAPLLSIL
                     EKGTIKLKINDEFKMFEIESGVVEVKDNKIRVIID"
     gene            complement(16316..17818)
                     /gene="atpD"
                     /locus_tag="PKI65_03190"
     CDS             complement(16316..17818)
                     /gene="atpD"
                     /locus_tag="PKI65_03190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOB77480.1"
                     /translation="MSEYKITGKVIQVIGPVVDVAFESEKDLPNIYDALEIKRENGES
                     LILETQQAIGENSVRTIAMDSTDGLYRGMSVYNTGEVISMPIGNEIRGRLFNVIGNNI
                     DGLPQVVTQKRSPIHKDPPKFDELSTETEVLFTGIKVIDLIEPYVKGGKVGLFGGAGV
                     GKTVIIQELMNNIAKQYSGFSVFAGVGERTREGNDLLREMIEAGLIKYGQEFIEEMEK
                     GGWDLSKINQDEFKDSLLTMVFGQMNEPPGARARVALSGLTLAEYFRDGDDISGGRDI
                     LFFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLATEMGLLQERITSTKRGSITSVQA
                     IYVPADDLTDPAPATTFAHLDATTVLSRQIAELGIYPAVDPLDSTSRILSPDIVGEEH
                     YKTAQQVKEILQRYKELQEIIAILGMEELSEEDKLIVHRARRVQRFLSQPFHVAEQFT
                     GVPGVIVNIEDTIKGFQMIINGEVDDLPEAAFHLVGTIEDAMEKAKKLSN"
     gene            18095..21175
                     /locus_tag="PKI65_03195"
     CDS             18095..21175
                     /locus_tag="PKI65_03195"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOB77481.1"
                     /translation="MKKYTLWNNVIGWIIFGIAAFVYLSTIEPTTSFWDCGEYIATSY
                     KLQVGHPPGAPTFQLLARFFTLFTNDVTKVAMCVNCMSALMSAFTILFLFWTITYFAS
                     KILIKNEESYSLYNIIAILGAGVVGALAFAFTDSFWFNAVEGEVYATSSFFTAITFWA
                     ATKWERNADDPHSFKWLIFIVFLIGLAIGVHLLNILAIPAIVLIFYYRKYKPTTKKTI
                     WALIIGIVILGLLFMVIIPGVVWLAGKIEIFFVNTLGLPFNSGTIFFFIFVIGGIAFA
                     IWYTQKHKLLLANSIWLALAFLLIGYSTFLILPIRSNADTPINENAPKTAPALLAYLN
                     REQYGTTPLLFGPYFNAPYAPRSEWKNRSPVYEKDEQAKKYIIIDKREKSMPAYDKRF
                     TTFFPRMWSNQESYHENAYRDWGKVKGVPIRVNVDGETKVIYKPTFIENLRFFFTYQV
                     GHMYVRYFFWNFVGRQNDIQGHGDPMNGNWESGITFIDNIHLGNTTEYPTYMKENRAR
                     NHYYFLPLILGLVGLFYQLNKNSKNTLVIFMLFFMTGLAIVVYLNQTPYQPRERDYSY
                     VGSTYAYAMWIGLGVLAVSDGIKKLIKKENIAAPIIATIITLGLVPCIMASENWDDHN
                     RSNRYTARDIAKMYLDSCEPNAILFTVGDNDTFPLWYAQEVENHRTDIRVANLSLLQT
                     DWYGDQMKRKVYLSDPLPISMDHIAYRSGTRDIIYIFDQIKEPINLIEYFDIINNNND
                     AFALNDPVFGRLDYLPGKVFYVPVDKEKVLSLGIVPEKYKDSIVDSVFININRTAITK
                     AQLFLLDILAHSDWERPIYFSTTSGQESYMGLEKYFFQEGITYHLLPVKANSIDMYAG
                     VVNTEVMYDNFMKFKWGNIKDPKVYLDETNLRMAKTLRSIVSGRLATYLINENKRDSA
                     LKVLNIAMEELPENKVPYDLYILPIIESYLNVGALDLAKPIMQRTADISIEYLKYIYS
                     LPAKKHRLLDLDKQQYLFLLHSVLRYADQYKMKDIMDTYMNQFEHYYHLYTTKG"
     gene            complement(21403..22155)
                     /locus_tag="PKI65_03200"
     CDS             complement(21403..22155)
                     /locus_tag="PKI65_03200"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOB77482.1"
                     /translation="MNIEIKNFKIIESTNVEAIKNWQNYSLDSFVLFNALYQTNGKGQ
                     GKKKWYSEANNNIAATILWRFKDPINVKDIPLFLFSEWVAVNICKELNKILNEDKFTI
                     KWPNDIVINNKKICGILIENVIMGNEIHALVAGFGININQINFPDDIPNATSLKLLTN
                     KDYDVYEIVMSIFNSLIDTTNFIFYDGETIHQEYKKMALKINIVDGLLLESREKLRVQ
                     IMDILEDGAIIVKNFNNNMINKFYHHQFRVIY"
     gene            22227..22613
                     /gene="rsfS"
                     /locus_tag="PKI65_03205"
     CDS             22227..22613
                     /gene="rsfS"
                     /locus_tag="PKI65_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOB77483.1"
                     /translation="MQNKNFIKDVNNDIISKENILKTLYLLKAQEIVSMNMGQLPNSI
                     CDFFIIAHGTSSLQVRSIAENLIYELRNHFCYRPHHVEGLQNAEWILIDYGDIVIHLF
                     LESSRRFYDLESLWADTTIEKFDELL"
     gene            22750..24741
                     /gene="ftsH"
                     /locus_tag="PKI65_03210"
     CDS             22750..24741
                     /gene="ftsH"
                     /locus_tag="PKI65_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOB77484.1"
                     /translation="MAQKTKSNKSFYWVYIIIIAIFAILFFYTPDKTQTEEISWFKLE
                     ELIKEKNIEKIEVVNNEYAIVYPKKKTETKSKKDNIFSSPVEPQIMYKYPFISAENFE
                     KQLYETIYTAIKNDTIGKEPAEQKKVIESYQIPITAKKVKNYWGDIFGWIITFALIIL
                     LWFFIARSLTKSAPGGGVFNVGKSKPKVVEGDKKIDVSFKDVAGLEEAKIEVQEIIEF
                     LKNPTKYTRLGGKIPKGVLLIGPPGTGKTLLAKAVAGEAGVPFLSMSGSEFVEMFVGV
                     GAARVRDLFEQAKAKAPCIIFIDEIDAIGRARGRTLITSANDERESTLNQLLAEMDGF
                     SPNVGLIVMAATNRADVLDKALLRAGRFDRLIYVDLPELKEREEIFKVHLRPLKLADD
                     VDVKLLARQTPGFSGADIANVCNEAALIAARKNKNAVDMSDFNDAIDRIIGGLEKRSK
                     IITPEEKKIIAYHESGHATVSWFLQYAHPLVKVSIVPRGKALGAAWYLPTERNIITKE
                     QLLHQMASALGGRVSEQVFFGDVSSGAMNDLEIVTKQAYAAVTYYGFSDEIGTLSFYN
                     SNPDEFFGYQKPYSEKTAEKIDKEAKKLIEDAYNIAYNIINEHKDQLSQIAQQLLKKE
                     VIFREDVEAVLGARPWPDPDAELFSDQETNYNEANTNEN"
     gene            24768..25652
                     /locus_tag="PKI65_03215"
     CDS             24768..25652
                     /locus_tag="PKI65_03215"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOB77485.1"
                     /translation="MMAVEVDNKKKFSDLPKRSLTAIIYGVLWLIGIYFGGWVLALVT
                     YLFFSIATFELIKLISTSKSEKNYFYTISISAIILLIISQIILFFNYKKFSFLKTFFI
                     NIPSFKYLILLSIIIIFIILILEIIYIVFYNKQSITNYFKFLAFFIFWIIIPIISIQF
                     LNTFNHLLISIVSIWVFDTMAYIMGVLAGKRPLAKDISPKKSIEGLLYGAFLTYIIFV
                     IYFSFIEKRTDLYWIVAISPFLATIGDLLESKFKREIGVKDTGNILPGHGGMLDRLDS
                     IIFAIPFIVILNIIVIIF"
     gene            25659..26324
                     /locus_tag="PKI65_03220"
     CDS             25659..26324
                     /locus_tag="PKI65_03220"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOB77486.1"
                     /translation="MGKGLHPSGYNNIIIGSVIIILIAIGLILFPFHLLIKVLILITA
                     IILIIWIALFFRKPKKTVNNAADNEIYSPAFGKVVVLEKIYEDRYFNEERIQLSIFMS
                     MSNIHLNFMPCNGKLLNKEYQKGHNYHARKAKASMLNEMSTLLIESNTGKQILMRQIA
                     GAAARRVVTFIDKNESANAGDTIGVIRFGSRVDLILPVDTELYIKVGDKVKGGWTLIG
                     RFK"
     gene            26412..27161
                     /gene="lptB"
                     /locus_tag="PKI65_03225"
     CDS             26412..27161
                     /gene="lptB"
                     /locus_tag="PKI65_03225"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOB77487.1"
                     /translation="MILKAENLVKIYGKRTVVKGVTFDVKQGEIVGLLGPNGAGKTTS
                     FYMIVGIIKPNEGKVFLDDLDITSYPMYKRAQLGIGYLAQEPSIFRNLSVENNIRVAL
                     EFTNLSQKQQDEKLEQLLNEFGLQHIRKSKGIQLSGGERRRTEIARSLAIDPKFMLLD
                     EPFAGIDPIAVEDIQSIIYNLKTKGIGILITDHNVHETLSITDRAYLLIDGSILKSGS
                     AEELANDEEVRRLYLGTNFELRKYFECNNNE"
     gene            27183..28457
                     /gene="purD"
                     /locus_tag="PKI65_03230"
     CDS             27183..28457
                     /gene="purD"
                     /locus_tag="PKI65_03230"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOB77488.1"
                     /translation="MKALVLGSGGREHAILSKLQNELGQNNTFIMPGNGGTKPFSING
                     DLKNFEHIKKIVLDNKINLVICGPEQPLADGIADFFDSDIQLNKIFFVGPIQRAARLE
                     SCKSFAKQFMQKYHIPTANFASFNYNQYEDAIDFLSTISSPFVIKASGLAAGKGVIIE
                     NNINDAKQTIEKLFFKNYLGEAGKEIVIEEFINGIEMSVFIVTDGDNYILLPEAKDYK
                     KIGEGDTGANTGGMGAASPTGFFNGNLKNKIINNIIEPTLIGLKDSNIDYKGFLFFGL
                     MINADDPYLLEYNVRLGDPETQVVMPKIKSSFSDLCISINEKKLNNYKILTDDLHYCC
                     ITLAAKGYPFDYEKNKLIFLDKENINSIIFHGGTKINEQGQLVTSGGRVLYVVGYGDT
                     IDSARDMAYKDIERIDFDGIYYRRDIGIDLLK"
     gene            28778..29863
                     /gene="serC"
                     /locus_tag="PKI65_03235"
     CDS             28778..29863
                     /gene="serC"
                     /locus_tag="PKI65_03235"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOB77489.1"
                     /translation="MRKHIFNAGPAVLPVPVLEKVSKSVQDLDGIGMSILEISHRSKE
                     FEAIINDAINLMKEIMQIPEGYHIIFVGGGASTQFGMVAMNFLNKKAAYLETGNWAKK
                     AIKEAKLFGEVQIVATSADKNFTYLPKNFIIPTDVDYFHITTNNTIYGTELKTDLNSP
                     VPLVADMSSDICSRPIDISKYSLIYAGAQKNIGPAGVTIAIIKDEFLNHQVTDRIIPT
                     MLNYKTHTENNSLYNTPPVYAIYVVKEVMNWLKNEIGGLENMKKINEEKAKILNDYLD
                     NSKMFVPTVPDKEDRSLMNITFVMQEPYKELEATFIDYAKTHGIVGIKGHRSVGGFRA
                     SMYNALPVDSVKALVQAMEDFEKTNLK"
     gene            29915..30838
                     /locus_tag="PKI65_03240"
     CDS             29915..30838
                     /locus_tag="PKI65_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOB77490.1"
                     /translation="MNKIVVLTEKPFAADAVNKMTEIAKAENFEIKFIEKYTSKEEAY
                     KGVKEANALIVRSDIVDKDLISHAPNLKIVVRAGAGYDNIDLNACTERNIVAMNTPGQ
                     NSNAVAELALALMLYISRNQFDGSVGTELKGKTLGLHGFGYTAKALATLVKGFDMKIY
                     GHRRSKRDDEYIAYNVTKAESVEDLYKNSQYVSLHIPANDATIKSINYNLLSLMPKNA
                     VLVNTARKELINEDDLLKVLEERPDFRYVSDVAPDCKNIIAEKYPGRCYFTPKKLGAQ
                     TKEANNNAGLAAIRQIIAFFKTGDTTFQINK"
     gene            30882..31343
                     /locus_tag="PKI65_03245"
     CDS             30882..31343
                     /locus_tag="PKI65_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOB77491.1"
                     /translation="MEENRPMNFFRFEDLRIYHKTLDFVSWVYNATKPFPDYASILRN
                     DMVEAAQEITINIAEGSGRNKNQFIYYLKMARSSIRKSVVLITIAQKEGFITDDQHEE
                     AKNLLIEISKMLGALISSLQRNKKTDRSASEEVEEEEEEEDFDENTGNELI"
     gene            31491..32738
                     /locus_tag="PKI65_03250"
     CDS             31491..32738
                     /locus_tag="PKI65_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOB77492.1"
                     /translation="MAIFRPFKALRPPKELAPKVAAKPYDVINTEEARKEAEGNPYSL
                     LHITRAEIDLPVGTDEHDPRVYEKAKENFQKFIENKWLIPDDDAYFYIYAQTMWNKTQ
                     YGIVGCASIDDYFNNVIKKHELTRRDKEEDRMKHVRVTNANVEPVFFAYKSVAELDSL
                     IMNYANEHKPEYDFIANDGVGHQFWVIKEKELIDKIQKLFAEMPALYVADGHHRTAAA
                     ALVGKEKREQHPNYTGEEDFNYFMAVVFPDNQLTIIDYNRVVKDLNGLTPQEFLDKLS
                     EYFDIENKGKNEYKPNKLHNFSMYLDGNWYSLTAKKGTYNDNDPIGVLDVTILSDLVL
                     NNILGIKDLRTDKRIDFVGGIRGLGELKRRVDSGEMAVAFALYPVSMKQLMDIADSGN
                     IMPPKTTWFEPKLRSGLSIHLLD"
     gene            complement(33109..>33317)
                     /locus_tag="PKI65_03255"
     CDS             complement(33109..>33317)
                     /locus_tag="PKI65_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77493.1"
                     /translation="SEFTNWIYFKFRGVATKYLQNYIMWYVVMGKYLTGKTVSNTGQM
                     LNLASSDRWAWYRYKELINLTYVD"
BASE COUNT        11908 a         4662 c         5088 g        11659 t
ORIGIN      
        1 ctataatctc tattccaatt taaataaata gatccaggaa atttagcttt gtaaaaattg
       61 cgcttcgtaa acgcgatttg tgaacggagc tacataaaaa tatcctttgt tttactttac
      121 gtattttata gcataattaa ataattagac gcggtgtata tagtgtacaa gtagtcaaac
      181 gatttcaagg tttggttgcg acgatttaga ggagtggtta ccaaatacct ccagagctat
      241 ttgatgtggt ttgtaatcac acataaatac ttgacaagtg agatagaatc tgacattggt
      301 cggcttttaa acctcgcatc ctcggacaga tgggcgtggg atgagtattg gcggttggtg
      361 aatacgaaat atagataaac tacttcaagt aaaacaatga ttaaatcttt tatagcctct
      421 agcctctatt taccttcacc atacaagaga atctttgcta aattaaaaaa tcatagcttt
      481 tttaattgtt tgaattgaaa ataatatttt ttatctcata aaaaatttcg aattttgcat
      541 aaaaaatgtt tatagagatt ttaaaaagta aaattcacag agcttttgta actgaagtaa
      601 atattaatta tcaaggaagc atcactatcg atgaggatct aatggatgcc gctaatatta
      661 tagaaaatga aaaagtacaa gttttaaatt ataacaatgg tgtacgaata gatacttatg
      721 tgataaaagg caaaagaggt agtggtgaga tatgtattaa tgggcctgca gcacgtaatt
      781 ttataataga agaccctatc atagtgctca gtttcgcaac tattgactgc gaagaagcta
      841 aaaaaatcac tcccaagatt atatatgtgg atcaaagtaa taaaattata tccaagtgaa
      901 gccaaaggta aaaacctatt tgcaaacatt tatatttctt gcaataggta tttttcttat
      961 attttggttt ttaaataagc tatcgccagc agaaaaacaa gaaatatggc attatttctt
     1021 acaagctaat cctttatgga tgataatagc aatgtttatt ggtattatat cccacatttt
     1081 tagggcatta cgatggaatt tgctaataga atcagttact aaaaagccta aaattaaaaa
     1141 tactttctgg gccgtaatgt ctggatattt agttaattat gctgtaccca gactaggtga
     1201 aatcactcgc tgtgctttgc tcggaaaaaa agaaaaaata gctgtagaca cagtattggg
     1261 cactatgata tctgaacgac tttttgatgt gatttgttat gttattattt ttatattggc
     1321 ttttgtggct cttactgcta aagtaatagc cttcttagat aattataata agccaacttt
     1381 tatctcttgg caatttgctc tcattttgtt actagctgcg atattaatag tagtggctta
     1441 taaactatgg cgcaaatata aacctaattc taaacttggt caaaaaattt ctgattctat
     1501 tcacagattt atcgatggtg gcaaatctat tattaatctc aagaaaaaag gacttttcat
     1561 agtttactct atcttgatct gggtatgtta tcttttaatg acttattttg cttttttaac
     1621 gatagatgcc acatatcatt tacctattga tgcagctttt gcagtattag cacttgggac
     1681 tataggtatg atagttatac agggcggtat tggggtgtat cctattattg tttcacaagt
     1741 attgatcctt tatggagtcg ataaagtagg tggatatgca ttaggatgga tatcatggat
     1801 tatacaaact gtgatcgttt tgattttagg cttaataggt tttattattt tatctaaaaa
     1861 aaataaatat gaacaaatac cagattttaa taaataagat tcttacactc aatgatttac
     1921 ttttaaaaat taaaaattgg cgagataatg gagatatcat tgtatttact aatggttgct
     1981 tcgatctaat acacgtagga cacatagaat atctaacaaa agccgctgat atgggcgata
     2041 gactgataat aggtttgaat actgatgaat ccgttaaaag attaaaagga cctgacagac
     2101 ctattttaga tgaaaatgct cgtgccaaaa ctctttcagc tttgtttttt gtagatgttg
     2161 ttacattgtt taacgaggat actccttatg aattgataga tgctattaaa cctgatatct
     2221 tagtaaaagg cggagattat gaaatagatg agataatagg tgctgatata gtaaaaagtt
     2281 atgatggcaa agtagaaatt atcccattag tcccaggcta tagtacaact aatattataa
     2341 acaaaataaa taaattatat gataacaatt aaataattca gaagtattaa ttttaaataa
     2401 tttttttaaa taatgaatat gataaatcaa actattttat ttctagattc tacgcacgaa
     2461 tatttgcccc aacaatttat aaataatggc tatgaagtaa taatgcatta tgatttaccc
     2521 gaaaaattga attttgatat tagaaaaaaa gtatatggca tcatcatacg tagtagatta
     2581 attttagata aggataaaat tgatttattt ccaaatttac attttatagc tcgtgctgga
     2641 tcaggaatgg aaaacattaa tatagaatac gctaaaagta aaaatattta ttgtataaat
     2701 tctcctgagg gaaatagaat agccgtagca gagcatgcta tagggatgtt attatcatta
     2761 atgaataata taataaaagc taatttagaa attaaaaatg gactatggcg tagagaagct
     2821 aatagaggct atgaattaga tggtaaaacc tttgggatta taggatatgg aaatgtaggt
     2881 acggctattg cagaaagatt gcaaggcttt aatatgaaca ttatagccta tgacaaatac
     2941 aagcacgatt ttagtgatca aactgtcaca gaagtttcat tggatgagct acttgagtct
     3001 tccgatttta tcagtatgca tgtgcctctg actagagaaa ctatttatat ggtaaatgat
     3061 gatttttttt ctaaattatc taaatctatt tggtttataa atacaagcag agggcagata
     3121 gtgaatacca aatctctttt aaacgccatc gatagtggta tagttttagg tgcttgttta
     3181 gatgtattag aatgggaaaa ccactctttt gaaaatatcg aaaacatgct atctcatcct
     3241 gatgatattc taaaaagagt aataaatcat ccccaaatat tactaacccc tcatgtagcc
     3301 ggatggactt ttgaatctaa taaaaaacat gctgaaattt tgtggaaaaa attaaagaat
     3361 ttaaaattag tttagagtca cagttccttc tataataaaa tttattattt tgataaaaaa
     3421 ttgtttaatt atatttacac tgctttgtct tcatttagat ttgcattttt tgtaataaaa
     3481 ttttatctat gtgcaagcaa atatcattaa atactatatc tacatctcga tggccacata
     3541 tacttacaat atttttgtta gtttgtttat aataatctaa aactggtaaa gtatccctat
     3601 aataatactt taatcttttt gaaatgatag cttcattatc gtcagttctg ccttcttttt
     3661 gcgctctgta taataaacgg gattttatta tttcttctgg tacatctaat tttattatta
     3721 gatccaatag taaattatat ttttctagct cttcatctag catttttgct tgacgcaatg
     3781 tacgagggaa tccatcaaat atgatacctt ttgtattgca attttctttt aaaaaatttt
     3841 caacaatttc ttccattata tcatctggta caagtgcacc agagtttaca atagatttta
     3901 tttttttgcc aattttagtt tgctttctta tctcttccct taataattca cctgtagaga
     3961 aatggataaa tccatattta tctgctatct tttttgcttg tgtaccctta ccactaccta
     4021 atggaccaaa taaaactata tttatcatat ttcttttaat ttgtaaatat ctaaataatt
     4081 tcttcctagt ccattataat ccaaaccata tcctactaca aattcattct taatattaaa
     4141 gccaatgtaa tctatcttta ttttatcatt aaatttaaaa acttctggtt tgaaaagtaa
     4201 cgtggctatt gatatttttt ttggtgaata ttttgataaa tcatttaata aattttttat
     4261 agtcaaacct gtatctatga tatcttctaa aattataata tatctattat ttaaatctgc
     4321 ttttatacta ttggctatta gacttacatt accagaacta cttattccaa cataagaact
     4381 tacttttata aaatctactt cacaattata tgggaaacgt tttacgattt cggttgtaaa
     4441 tataaaagac ccatttaata ctgaaataaa cacgggcatt ttgtctgcgt aatcttttat
     4501 tatttgaatg cttaattctg ttattctttt atttatttgt tcctcagtaa taaataactc
     4561 aaacaacctg tcatgtattc tcatcgtttt catagcaata attttattac aaaaattttt
     4621 gataaaatta ataaatttat tggttaaaaa atttttttta gatacttttc ggagtgaact
     4681 caattataaa tatataaata tctgccgtca aaattatttt tatataataa agcaggttta
     4741 gtagcaggtc cattaattat actaccatct attattaaaa atttagatcc acagcattca
     4801 tcttttacaa ataatactgt gctgtcatct acatctaatc tttcgcaatc taataaaggc
     4861 ttataagggc atgctcgctc catagtaacg aattcatctt gtgattttct gtatatgata
     4921 attcctttgt aacctccagt aatatacata taaccaccta ctacattaat atcgctatat
     4981 aaagcatcat acacatcaat tctaatattt acaggcacat aaggaatata attatcatca
     5041 tctttacaag cataaatcaa aaaagttaaa aaaatcaaaa aaaataatct atgtttttta
     5101 aacatttatt atcctttagt tttaggtggt ttatgttgct gtttatattg ccaatttttt
     5161 atccaacgtt taataaaaaa ttctttttta ttttcacgat ttcttttggc ttgtttttga
     5221 gcatctttca tggcttctct tgtttcggga gtctgattat tcatctgttg ttttacagct
     5281 ttttgataca aaatggtatc atctttttga cgtttctttt tttctttttc tatttttttc
     5341 tcttgactat tgatactagt agattgactc ttacaagaat aacaagtcat tgataagcct
     5401 gaaaaaagta taattattaa gaatattatt gttttttgat tggatatttg tatcattaaa
     5461 tataaatttt ttttttaaat ctttgttgca aagatataaa tttattaaat tctaaaaaaa
     5521 attatttaat actaaaaaaa gataaaatta aaaataaatt gaatagtaaa aatcaaaata
     5581 caacaaattt tttaaatctt tattacaaag atataaattt atttttttta taaaactttc
     5641 ttatttatgc gaaaaaataa ttaaatttta taatcacttc actttgtccc ctggtttagc
     5701 attatcatct gcacacacaa gcatcagatc tccatttttg ttttctacaa atagtaccat
     5761 accctcagat tctacacctc taattttctt tttttctaaa ttggcaacta atattacttt
     5821 tttattaatt atttgatctg ggcttatatg ttgagctata ccagaaacta tagtacgtat
     5881 ttgaccacct atatttattt gcaattttaa aagtttatca gcattagata tatactctgc
     5941 agagattact tggccaactc tcaggtctat tttttcaaaa tcttcccacg agattatagg
     6001 tttcaaatca tcaagtcgat ttactacttc tactgcatca gatttatgaa gtttatttaa
     6061 ttgattttct ataacctcat cacttatctg ttcgaaaaga atgataggtt gttctatacg
     6121 tgcaccagtt tttaataaag gagtccctat tttatcccag tctaatagat ttatgtgtaa
     6181 aatattcctt agtttttgag ctgtgaaagg aaggaatggt tcgcttaata gcgataaatt
     6241 agccacaagt tgtaaaatat tataaataat acctttggtg atattaggac ttgttttagc
     6301 aagtttccaa ggttcttttt cagtcaaata tttattacca aacctagcta catccatcat
     6361 agagtttaga gcttcacgaa atttgaattg ttctaaatag ttagttacct catcccgttt
     6421 ttttgtaatg aaagaataaa tcatttcttc atcttctatt aaaaagtttt cgggtactaa
     6481 accactatag tatttatgcg tcaatacgag agcacgattt accaaattac catagatagc
     6541 tgctaattca ttattattat gttgttgaaa gtttttccaa gtgaaatcac tatctttggt
     6601 ttcgggcata ttgatagtga gcacatacct caatacatct tgtttattag gaaaatcgac
     6661 taaatattca tctagccaaa ccgcccaatt acgggaagta gagatttttt tgccttctaa
     6721 attcataaat tcattagctg gtacattttc tggtaatata taagagccct ccattttgag
     6781 cataactgga aatatgatac aatgaaaaac gatattatcc ttgccgatga aatgtatcag
     6841 tttggtatct tgatttttcc aataaggttg ccaatcttta ccagtttttt ttgaccaatc
     6901 tatagaagct gatatataac ctaaaggagc ttcgaaccac acataaagta ctttgccttc
     6961 ggcgtcaggt aggggtacag gtactcccca gtccagatct ctagtaacag ccctgggttg
     7021 cagtccttga tctaaccaac ttttacattg accataaaca ttaggtttcc attctttgtg
     7081 tccttccaaa atccattctc taagccaacc ttcatatttg tctaatggca aataccaatg
     7141 taaagtttct tttaataccg gtgcagaacc tgataaggta gaacgtggat ttatgagttc
     7201 ctgcggactt agtgatgatc cacatttctc acattgatct ccataagctt cggtataacc
     7261 acaatgaggg caagtaccta ttatatatct gtccgccaaa aattgacctg cttcttcatc
     7321 ataatattgt agaatatttt gtgtaaaaag ttcccctttg tttaataatt ttagaaaaaa
     7381 atcctgactt agttgataat gtatggggtt ggaggtacgg gaataaatat cgaaactaat
     7441 accaaaatct tcgaaagatt tttttatttt attatgataa tgatctacta tctgctgtgg
     7501 cgatatattc tcttgtctag ctcttatggt tatcggtaca ccatgttcat cgctacctcc
     7561 tacaaataat acatctctac ctctcattcg caaataccta acatatatat cagctggcac
     7621 atatacacct gccaaatgtc ctatatgaat cggaccatta gcatagggaa gtgctgttgt
     7681 gacagtatat tttttataat ttcttgattc agccatacaa ttatttttat tgtgaaaatg
     7741 caaaaataat aaaaattatt tgtgaaataa agtaaaaaat aattttgtaa ttatgcattt
     7801 tattaataat agggtatcta tttacactga aagtatcatt aacattcttg ttgtcttata
     7861 aatctcaaat aaaagtatga atgcactaaa tccaaattat catcgcagtt cactacattt
     7921 gccttgttat gattatttac aagagatcgc atattatatt acaatatgca cctaataata
     7981 ggcaaaatat atttggcgat atcaaaaata atgaaatggt tttttttaaa tgagtctgat
     8041 aaaattgcaa ataaatattg ggaataaatt tcaacacatt atccaaatac acacatgggt
     8101 aaatatgtaa taatgccaaa tcatattcat taaataataa ttattggtta aatgcctaat
     8161 tcatagtatt tagagttgta ggctgatttt tgtattaatt attttcgata tccatatgat
     8221 aataacttta tgataataac tttagaaatt ttaaatactc tacgttatta tatgagaata
     8281 aaaaaattct aaaacaaaat ttcattccat tggtaaacaa attataacta ctaaaattaa
     8341 aaatcaaaaa tatattttgt taaatattta ataatcattt actttgcaaa aagattcaaa
     8401 aaataaaaat tcatgaattg ggaacatatt aatgaagata tagataaaat tattaaaaat
     8461 gcatctataa ctcaaaaaat tgcaaaggaa ttgatcagtt ttatagactt aactactctc
     8521 gaaggtacag ataataattt agtggttaag gaactaatag cccaagctaa ggagagttat
     8581 caaaaaacta atggcaaggg tgtagctgca gtatgtgtat atccagtatt tgcggatttc
     8641 gctgccaatg agttagctaa tacaaatatt catttagcat gcgtggcagg tagttttcct
     8701 agtggccaat cgccattaca cgttagaata gctgaagtgg agcatgccgt agcacatgga
     8761 gctcaagaga tagatatggt tatatccaga ggagccgctt tagctggcga ttttgatttt
     8821 gtaaaatacg aaatagctct gcataaaaaa gcttgtggta gtgcccattt aaaagtaata
     8881 ctcgaaaccg gagaattaaa agatcctgat ttaatataca atattagtat gttagcaatg
     8941 gaaaatggag cagattttat caaaacttct actggcaaaa cctcaccagc agccacatta
     9001 gatgcggctt ttgttatgtg ttgtgctata aaagaatatt atgctcttac taacaaaaaa
     9061 attggtttta aacctgcagg tggtattaga gaacctcaag aagcatttaa ttattactta
     9121 attgtaaaaa atattttggg aatagattgg ttgactccta atctttttcg tatcggtgct
     9181 agtaaattat tgttaaatct ttataaattt tcagaattat gaattcagat aagttattta
     9241 atgacctcat tgatttgggt ataaaaacaa gtaaagtaca ctacaatttg tcttatgaag
     9301 aattatttga gcatgagacc cgtccagatt taaaagatta tgctaaaggt tttgtgacta
     9361 aatgtggtgc tgtagctgta gataccggta tattcactgg tcgttcacct aaggataaat
     9421 atgtagtata tgatgatact acaaaagatg tagtatggtg gaaaaatgaa ataaataaaa
     9481 caagtgataa taagcctatg tcccccacag tttggacaga gctgaaacaa cttgtagtta
     9541 attatttttg tgataaagaa ctttacgtta tggatgcttt cgtaggggca tctgagaagc
     9601 atcgattacc aattcgtgta atcacagata tagcatgggc tgctcatttt gtaaaaaata
     9661 tgtttattgt tcctactgaa gaagaattga aaaatttcga acctcaattt ataatgctgc
     9721 atgcacctcg tgtgacaaat cctaattggc tagaacataa gttaaatagt gaagtttttg
     9781 taacctttaa ttttactgat aaaatggctc tcgtaggtgg tacttggtat ggtggagaga
     9841 taaaaaaagg cttcttttct gttatgaatt attatctacc cctaaaaaat gtagcttcta
     9901 tgcactgctc agctaatatg ggaaaagatg gggatacagc tatatttttt gggctatcag
     9961 gtacaggtaa aacaacccta tctgcagatc ctaaaagatt tttgataggt gatgatgagc
    10021 atggatggaa cgaagaaggt atatttaatt tcgaaggtgg atgctatgct aaatgtataa
    10081 atttagatcc taaaaaagag ccccaaatat atcaagctat tcgcagaaat actatattag
    10141 aaaatgtagt ttataatcct gaaactggag aaatagactt ctctgacgct tctaaaaccg
    10201 aaaatactcg agcttcatat ccattatctc atattcctaa tgctatcata ccatctatcg
    10261 ggccacatcc taaaaatata atttttttaa ctgctgatgc tttcggtatt ttaccaccag
    10321 tagcagtttt gagtccagag gaagcaatgt attatttcat aagtggatat actgctaaat
    10381 tagccggtac tgaacgcggc ataatagaac ctatacctac tttttctgct gcattcggag
    10441 ctgcttttct tacattacca cctattattt atgctagatt attagctgat aaaatgcaaa
    10501 aacataatgc tcacgcttat ttagtaaata caggttggat agggggacct tatgggatcg
    10561 gaaatagaat agatatcgat gtttctagaa agattattca tgctatatta gatggttcta
    10621 ttttgcaaac aaattatgat attatgccat tttttaatct caaaatacct aaacacattg
    10681 aaggaataga tgataatata ttaaatcctc gttatacttg ggaagataaa gaagcttacg
    10741 ataaacaagc tcaaaccctg gctaaagctt ttgttgataa ttttcaaagt ttcactactg
    10801 atgatgaaac taaagaatta attgatttcg ggccaaaaat ttaatattaa tgtggatgct
    10861 gctttttgaa atataattaa atttaatgct ttatttaaat ttataaaaga taaaaaaagt
    10921 tttttggata attaatctga ttgattattg ctaattgagc ggaaagttag agttttacta
    10981 attttacaaa caagtttttt ttttaaaaaa accaacaaaa ttcctgttgt caaataaaac
    11041 tgtgaaagta aagaagtttt agaaattttt acaaataata ttgacaaata aatacgcttt
    11101 ggtcctaata aaaactgaaa tttagataca taatattatg aagtttatca aattattttt
    11161 taacaaaaaa gtcatttcat atttatcgat tttatcacat tttgcacttc atcgaagtat
    11221 aattcctgta ttcctacatt gtacttattt aaatgaactc tagtactatc taaaaaaaac
    11281 ttcatcgccg acatgtcctc tggatataaa ttagccaatt tatcatattt ccaagcaggt
    11341 aaatttatgt atttgtatct attattttct agttttttcc atgtccatga taataaatat
    11401 cctgcttgtg ataattgaag cccatctttg ttttttgtaa attccaagta tgctatagca
    11461 ccaccatcag tgtgtatcca cctttgattt gatatgaaat tgcccagtga ataatagact
    11521 atagattcga gatttttaac agtatctatt atacttttta tcataggttg aataacgtgt
    11581 ggatgcgaac caatgatata atgggcacca ttttttatgc aaaattcagc taatgttttt
    11641 tgataattat taggtaaacg ttgatattct tcgccccaat gaaaataaat aattatacca
    11701 tcaggattat aagatttagc cagcaataga tcatctttta tttcattagt atctattaga
    11761 ttgattatta ttgacgatgt tggtaataaa gcattagtgc cataggtata attgagtata
    11821 gctaagcgaa tgtcatcttt ttgaataatt aaaggatgat ttttttgaaa atcaattttg
    11881 tttttaaaag tacctgtatg attgatatac aaactatcta aaacgtttat tgtgcgtatc
    11941 agaccattta tacctttatc taagctatga ttattagctg ttactaaaat gtctacagag
    12001 gctcttttta atgcccctgc taggtcgtca ggagcactga aagcaggata gcctgtatat
    12061 ggagctccat taaaagtggt ttctagatta ccaattacga agtctgcgta attaaataaa
    12121 ggtaataaat atttgaaatt ttctgaatag tcataatttt ttccattcca cgctgaattt
    12181 atctgtggtg tatgctgcat tatatcgcct ataaagatta atttgatatt ttcactttta
    12241 tcatcttgac aatataaaat cgaaaaacta aataatgcta agaataaaac taaaattttt
    12301 ttcatttttt ttaaattaat aaaaattttt tttcagtaat ttttcaaatt tttcaggtaa
    12361 aaaatgaata tcattccaag atttttctaa atcatccaaa ttagcagctg gcatcggttt
    12421 taaatcgcta aacagtggta agcaattata atttttatcc attaataaat gatttactac
    12481 agctctcact tcatagatag tgcccacaca ctcaaaaggt ttagtttctt tcaatcctaa
    12541 taattcatcc ataattggtt ttaattttaa attagaaaaa atatctactc ccaatatttt
    12601 ttttatctcc tgaggatcta aataaacaga tagcatcaga gctacgaata aacatttagc
    12661 acagttagca caccaactgt catttttact gccaatatta caacttctga aagcataaaa
    12721 atattgaggg taatttgtaa ataatttcac tacttgcagc tcgtgtaatg gacgtagaaa
    12781 gctaaaatat tcaatttcat cattcaaata tttttttaca taatctctaa aatcattctc
    12841 aaattttaaa cttttagaat attgatgatt tacatagcta tctctcacgg tactctcatt
    12901 agcactgaat tcattactca gagctatata tttagcacct atcatataag cactaaagat
    12961 actataaaaa gctagcatag cactaaaagg agtgtgccca ttaaaaaaac cctttttatt
    13021 taattctaat aaggtgggat ctatcatacg atgtatttct ataatgtctg atctgggtat
    13081 atgagctata tctaaaatac gtaatgttgc ttcattagga ttaataatca aaggtattat
    13141 ttcaaaatca gatcgtctca gcaattctaa agttacaata gagtctttgc cgcctcctat
    13201 tggtactatt actttattat catccaagta tttcaaattt ggctttttat aagttttaaa
    13261 attattaata taaaaatgaa taaattcgtc tattttaatt tctatattat ttttataaaa
    13321 aaactccccc attccataat aataaagatt tttccaccac attgtttgat aatcactcag
    13381 aaaggaaaga ttaatattta ctattggtga gcaagtagcc ttccaataac tgatcaattc
    13441 aatcattcca atattaaaaa caaaagcttc tatttcatta aaatttaact ttttagcatt
    13501 caaagtttta ttaaatcgcc aataagaact tgggtgaaat gaaaatttac cattatcaaa
    13561 gtcgaaagaa atctttaatt tgtctgataa taattggatg ttatatgtat tataattgaa
    13621 atatggataa tttatttgaa attctaaata cttattcatt atttttttat taatttgtaa
    13681 acttttcggc acaatttttg tcaaaattaa taagaaatca tattatgatg aataaagagt
    13741 ataaattaaa aattaaacga aatgatatag agcctacaat aggcagagtt ttagtagcag
    13801 aacccactac atacgatttt ttctttagcc gctcaattgt actattatta gaacacgaag
    13861 atagggaagg atctgcagga gtgattttaa ataaaaaaat tagtgataat tttaaagata
    13921 tttataataa atctaaaata aaagcagatt ttccattata tttaggaggc ccagtagata
    13981 cgaataaact ttttgtttta caccgtctag gagatataat acccaaaagt ttcgaagtga
    14041 taccaggtct atggtgggga ggcgatgtga aaactatata tacactagta aataataaaa
    14101 tagctaataa taataatttg aaagtctttc aaggttatag cggatgggca cctatgcagc
    14161 taagagaaga aatgaaaaaa aattattggg ctgttacaga gcttagtata gacgaaatat
    14221 tcgaaatgcc tgtagaaact atgtggaaaa aatgcataga aaatctcggt cctgaatatg
    14281 aattttggca acatatacca gaaaatccaa cttttaatta acctgacaat tttacaaatc
    14341 aactactgaa aacgaataat tacttgatta taacttaata aaaataatta agttaacatt
    14401 cattttatta atttaggttg ttcctattct aaataaatat tattatataa taatatttgt
    14461 actgtaatat aaaaatatta aaagtttgaa tatatttgaa tatatattag acttcgtatg
    14521 aagcagtttg ctaagggtag ggaggttgaa aacaatcttt gttgtacttt acgtatttta
    14581 tactatcttt tagaaaaata gaaatatctt tggattgcag cgatttagag atgtggctac
    14641 caaatacctc cagagctaat catacataaa tactcgacaa gtgacattga atcagatatt
    14701 ggtagacttt taaaattaaa atctagaatc ttcggacaaa tcggataaat gagcgtagga
    14761 taagtattgg cggctggtga atacgaaata tagataaact acttcaagta aaacgatgat
    14821 ttattttcat tcatacacta ctatctatat cactattaat aaccaaatag ttaaatctat
    14881 ataaaaattt acatttattt actagagatt ctcattttga tagcttatat gcaaattttg
    14941 acatgaatat tatgttaaaa atattaaaaa attctttatt aagcgtaaca agtgagaatt
    15001 tttattattt atttttagtt ttgctttgat aattattaat tttttattat ctaagaaaaa
    15061 ttattatatt tgcatgtaaa caaaataata ttatgattga aaaaaatatt gagaaagctg
    15121 tagatgtttt agctaaaaaa gataatattg ttgctatgac aggtagtggt attagcgcag
    15181 caagcggtct aaaaacattt agaggtaaag atggattgtg ggaagagtat aatccgcaag
    15241 ctttagaaat ttcttatttt tatgaacata gagctgaatc atgggagtgg atatataaat
    15301 tatttttcga acctttgcaa tatgccaaac ctaatccagc tcattatgca ttagcggaac
    15361 tagaaaaaat gaataaatta aatgccatca ttactcaaaa tatagatggg ttacaccaag
    15421 cagcaggcag caaaaacgtt atcgaatttc atggtactac tagaaacttg atatgtacca
    15481 aatgtggagc agaatatagt agagaacaaa ttgatttttc taatttacct ccaaaatgta
    15541 ataattgtgg tgaaatatta aaacccgatt tcgtattttt cggagaaatg atacctcata
    15601 aagcagctga atatgcatac catcatgcta tgcattgtga tgctatgatg attataggca
    15661 cttctggtat tgttatgcct gcagcagatt tgcctcgagt ggctaaacaa aataatgctg
    15721 tacttattga aataaattta gaacaaaata atagtaaaat atttattcct gacattttta
    15781 ttcaaggtga tgctaccgta atattaccta aaatagtaga taaattgaaa caaaaattat
    15841 tataaaaaat tttaaatgca attcccttgt ctaattataa aatttaacta attacctaca
    15901 tctgataact agaaacttat taatactcca tgctaatact cgatgtctct aaatattaat
    15961 ttttctcttc ttgtgtctcc cagtatcttc taattccttg atattgtatt tgattaatgt
    16021 aatcaatatg tatatattaa tcaataatta ctctgatttt attatctttg acctctacta
    16081 ctccactttc tatttcaaac attttaaatt catcatttat ttttaattta atagtaccct
    16141 tttcaagtat agataataaa ggtgcatgat ttggtaaaac ctggaaagga cttacataac
    16201 ctggtaacca tatcgtatct actacacctt caaaaatgac ctcttttgga tttattaatt
    16261 taacatatag cgacataata catcatttaa aattttatta gcaaaaaaaa aattatcaat
    16321 tagataattt tttagctttt tccattgcat cttctatagt accgacaaga tgaaaagctg
    16381 cttctggtaa atcatctact tcgccattta taatcatttg gaagccttta atagtatctt
    16441 ctatgttaac aatcacacct ggcacaccag taaattgttc agcaacgtga aaaggttgag
    16501 ataggaaacg ttgtacacga cgagctctat gtacaatgag tttatcttct tcagaaagtt
    16561 cttccatacc tagtatagct ataatttctt gtaattcttt ataacgttgc aagatttcct
    16621 tcacttgttg agcagtttta taatgttctt caccaacaat gtctggactt aaaattctag
    16681 aagtactatc gagagggtct accgcaggat agataccgag ttcggcaatt tgtctactaa
    16741 gaactgtagt agcatccaag tgagcaaaag tagtagcggg agcggggtca gtaaggtcat
    16801 ccgcaggtac ataaatagct tgtactgatg taatactacc acgcttagta gatgtaatac
    16861 gctcttggag taatcccatt tcggtagcta aagttggttg atagccgact gcagatggca
    16921 tacgccctag taaagcagaa acttctgaac cagcctgaac aaaacgaaaa atattatcta
    16981 taaaaaataa tatatcccta cccccactta tatcatcacc atcgcgaaaa tattctgcta
    17041 aagtaagtcc actcaatgcc actctcgcac gtgctccagg aggttcattc atttggccaa
    17101 aaaccatagt taataatgaa tctttgaatt catcttgatt aattttacta agatcccacc
    17161 cacctttttc catctcttct atgaattctt gtccatattt tatcaaacca gcttctatca
    17221 tctcgcgtaa tagatcatta ccttctcgag ttctctctcc tactcctgca aatacagaaa
    17281 aaccactata ttgtttagca atattattca ttaattcctg tattattaca gttttcccta
    17341 caccagcccc accaaataaa ccgactttac cacccttaac ataaggttct ataagatcta
    17401 taaccttaat accagtaaat aaaacctcag tttcggtaga taattcgtca aatttaggtg
    17461 gatctttgtg gatgggggat cttttttggg taactacttg tggcaaacca tctatattat
    17521 tacctattac attaaataat ctccctctaa tctcatttcc tataggcata gatattactt
    17581 ctccagtatt ataaactgac atacctctat aaagaccatc tgtgctgtcc atggctattg
    17641 ttctgacaga attctcgcca attgcttgct gtgtttctag tattaggctt tctccattct
    17701 ctcttttaat ttctaatgca tcataaatat taggtaaatc tttttcactt tcaaaagcta
    17761 cgtccactac tggacctatt acctgtatta ctttacctgt aattttatac tctgacatta
    17821 ctatctattt tttataaagg caaagatatt atatttttgc tatatcacaa aattataata
    17881 taaaaaaata aataaataca atctatttaa tttgaaagaa tatttgtgta tttgttttat
    17941 taataaaata atattggccc ttttatctaa aatatgctta acaaaattat ttatgaaata
    18001 aaattatttt attactcaag attaattaaa aaatttttaa ttttcaaaaa ttatttaact
    18061 tcgcagaaat ttttaataat aaaaattatt aataatgaaa aagtatacac tatggaataa
    18121 tgtaattggg tggataatat ttggcatagc agcctttgtt tatttatcta caatagaacc
    18181 tactactagt ttttgggatt gtggagaata tattgccaca tcatacaaat tacaagtagg
    18241 tcacccacct ggggcaccaa catttcaatt attagcacgt ttttttactt tgtttacaaa
    18301 tgatgttact aaagtagcca tgtgtgttaa ttgtatgagc gcccttatga gtgcttttac
    18361 aatcttattt ttattttgga ctattactta ttttgcttct aagatattaa ttaaaaatga
    18421 ggagagttac tcgctatata atattatagc tatattgggg gcaggagtgg ttggagcatt
    18481 agcttttgct tttactgatt ctttttggtt caatgccgta gaaggtgagg tttatgccac
    18541 ctcatcattt ttcacagcta ttactttttg ggcagctact aagtgggaaa gaaatgctga
    18601 tgatccacat tcttttaaat ggttgatatt tattgttttt cttattggtc tagctattgg
    18661 agtgcattta ttaaatatat tagctatacc cgccatagtg cttatttttt attatagaaa
    18721 atataaacct acaactaaaa aaactatttg ggctctaata ataggcatag ttatattagg
    18781 tttattgttt atggttataa tacctggggt agtgtggtta gctggcaaaa ttgaaatctt
    18841 ttttgtaaat acattggggt taccattcaa ttcaggcaca atatttttct ttatatttgt
    18901 cattggcgga atagcttttg ctatatggta tactcagaag cataaattgt tattggccaa
    18961 ttctatatgg ttagcattgg ctttcctttt aataggttat tctacatttt tgatacttcc
    19021 tattagatct aatgctgaca ctcctattaa tgaaaatgca ccaaagactg ctcctgctct
    19081 attagcatat ctcaatagag aacaatatgg tacgaccccc ttattatttg gtccatactt
    19141 caatgcacct tatgcacctc gtagtgaatg gaaaaatcgt agtcctgttt acgaaaaaga
    19201 tgaacaagca aaaaaatata taatcataga taaacgagaa aaatctatgc ctgcctatga
    19261 taaacgtttt acaacatttt tccctcgcat gtggagtaat caagaatcct atcacgaaaa
    19321 tgcatataga gattggggca aggtaaaagg tgtccctata cgtgtaaatg tagatggaga
    19381 aactaaagta atttataagc ctactttcat agaaaatctt agatttttct ttacttatca
    19441 ggtagggcat atgtatgtta ggtatttctt ttggaatttt gtaggtcgtc aaaatgatat
    19501 tcagggacat ggggacccta tgaatggtaa ttgggaatct ggcattactt ttatagacaa
    19561 catacatcta ggtaatacaa ctgaatatcc tacatatatg aaagaaaata gagcgagaaa
    19621 tcattattat tttttgcctc tcatattagg gttagtaggt ctattttatc aactaaataa
    19681 aaatagtaaa aatacacttg taatatttat gctatttttt atgacaggtc tagctatagt
    19741 tgtttatcta aatcaaactc catatcaacc acgagagcgt gattattcat atgtgggttc
    19801 tacctatgca tatgctatgt ggataggatt aggtgtatta gccgttagtg acggtattaa
    19861 aaaattgatt aaaaaagaaa atatagctgc tcctatcata gctactatta ttactttagg
    19921 attagtaccg tgtataatgg ctagcgaaaa ttgggatgat cataatagat ctaatcgata
    19981 tacagctagg gatattgcaa aaatgtattt agactcctgt gaaccaaacg ccatattatt
    20041 cacagttggg gataatgata catttcccct atggtatgct caagaagtag aaaatcatcg
    20101 cacagatatt agggtagcta atttatcact attgcaaact gattggtatg gcgatcaaat
    20161 gaaaagaaaa gtttaccttt ctgatccatt acctatttct atggatcata ttgcttatag
    20221 atctggcaca agagatataa tctacatctt tgatcagatc aaagaaccaa ttaatttaat
    20281 agaatatttt gatataataa ataacaataa tgatgcattt gcattaaatg atccagtttt
    20341 tggtagatta gattatttac ctggtaaagt attttatgtg ccagtagata aagaaaaagt
    20401 attgagccta ggcatagtac ctgaaaaata taaagacagt atagtagata gtgtattcat
    20461 taatataaat cgaacagcta tcactaaagc tcaacttttt ttattagata ttctcgctca
    20521 tagtgattgg gaacgtccca tatatttctc cactacaagt ggacaagaat catatatggg
    20581 tttagaaaaa tatttctttc aagaaggtat aacttatcat ttattacctg ttaaagcaaa
    20641 ttctattgat atgtatgctg gtgtggtcaa tacagaggta atgtatgata attttatgaa
    20701 atttaaatgg ggtaatataa aagatcccaa ggtatatcta gatgaaacta atttaagaat
    20761 ggctaaaact cttcgcagta tagtttctgg tagattagct acttatctta taaatgaaaa
    20821 taaaagagat tctgccttga aggtgctaaa tatagctatg gaagaattgc cagaaaataa
    20881 agtaccatat gacttataca ttttaccaat tatagaaagt tatttaaatg taggtgctct
    20941 agatctagcc aaacctataa tgcaacgaac agctgacata tcaatagaat atctcaaata
    21001 tatctactcc ttgcctgcta aaaaacatag attattggat ttagataaac agcaatattt
    21061 gtttctatta cattctgtgc taagatatgc tgatcagtat aagatgaagg atataatgga
    21121 tacatatatg aatcagtttg aacattacta tcatttatat actactaaag ggtaaaaaca
    21181 atattttgat ctatattttt tttgaaattt gatttaagat gtttgattaa taatatttta
    21241 tccgatttat tatttagtaa tatgaacttt agactctaat tttatctaat tttactttta
    21301 ttaaaagaat aattaatttt attactttat aaataattct tttatattta ttaaaaaaaa
    21361 atatatttat tgtaatattt tttgaaatga tcagtttaaa atttaataaa tcactctaaa
    21421 ttgatggtgg taaaatttat ttatcatatt attattaaag ttttttacta ttatcgcacc
    21481 atcttctaaa atatccatta tttgcactct tagtttttct ctgctttcta atagtaaacc
    21541 gtctactata tttattttta atgccatttt tttatattct tgatgaatag tttctccatc
    21601 ataaaaaata aaattagttg tatctatcaa tgagttaaaa atagacatca ctatttcata
    21661 aacatcataa tctttattag ttaataattt taatgaagtt gcattaggta tatcatcagg
    21721 aaaatttatt tgatttatat tgatgccgaa gcctgctacc aaagcgtgta tttcattacc
    21781 cattattaca ttttctataa gtatcccaca tattttttta ttatttatta ctatatcatt
    21841 aggccacttg atagtaaatt tatcttcatt taaaatttta tttaattctt tgcaaatatt
    21901 cacagctacc cattcactaa acaaaaataa tggaatatct ttaacattta taggatcttt
    21961 aaatctccac aaaatcgttg cggctatatt attattagct tcgctgtacc attttttttt
    22021 accctgtcct ttaccattag tctgatataa agcattaaat aaaacaaaag aatctaaaga
    22081 ataattttgc caatttttaa tagcttctac attagtagat tcgattattt taaagttttt
    22141 tatttctatg ttcattacaa tattctttag taattttgca aaaatattat aaataaaaat
    22201 aaaaataaat aaaaaaggga aagtgaatgc aaaataaaaa ctttataaaa gacgtaaata
    22261 atgatataat ttctaaagaa aacattttaa aaaccttata tttactaaag gcccaagaaa
    22321 ttgtaagtat gaatatgggg caattaccaa actcgatttg tgattttttt ataatagctc
    22381 atggtacttc atctctgcaa gtacgtagta tagcagaaaa tttgatttac gagttacgaa
    22441 accacttttg ctatcgtcca caccatgtcg aaggtttaca aaatgcagaa tggatactta
    22501 tagactatgg cgatatagtg attcatcttt ttttagaatc atctcgtaga ttttatgatt
    22561 tagaaagttt atgggcagac actactatag aaaaatttga tgaactatta taatattaaa
    22621 atagtaataa atagtattaa ttgattataa atttaattag taattttgat tttcaatttt
    22681 taaaataata atttgataat tattgctaaa aaattaaata gtatattttt tgaacagaaa
    22741 taagtaatta tggcacaaaa aacaaaaagc aataaatctt tttattgggt atatataatt
    22801 ataattgcaa tatttgcaat attatttttc tatactccag ataaaacaca gacagaagag
    22861 attagttggt ttaaattaga agagctgata aaagaaaaaa atattgaaaa aatagaagtt
    22921 gttaataatg aatatgctat tgtatatcca aagaaaaaaa cagagactaa gtctaaaaag
    22981 gataatatat tttcttcgcc agtagagcca cagataatgt ataaatatcc atttattagt
    23041 gctgaaaatt ttgagaagca actatacgaa actatatata ctgctataaa aaatgatact
    23101 attggcaaag aacccgcaga gcaaaaaaaa gtaatagaat cttatcaaat accaataact
    23161 gccaaaaaag taaaaaatta ttggggagac atttttggtt ggattatcac atttgcactc
    23221 attatacttt tatggttttt tattgctaga tcattaacaa aaagtgcccc tggtggtggt
    23281 gttttcaatg taggaaagtc taaacccaaa gtagtagaag gcgataagaa aatagatgtt
    23341 tctttcaaag atgtggcagg cttagaggaa gctaaaatag aagtgcagga aatcatagaa
    23401 tttctaaaaa atccaacaaa atacactaga ttaggtggta aaataccaaa aggtgtttta
    23461 ttaatcgggc ctcctggtac aggaaaaact ttgctagcca aagctgtagc tggagaagca
    23521 ggggtgccat ttttgtctat gagtggtagt gaatttgttg agatgtttgt aggtgtaggt
    23581 gctgccaggg ttagagattt atttgaacaa gctaaagcta aagctccatg tattattttt
    23641 atcgatgaga tagatgctat aggtcgtgcg aggggtagaa cgctcattac tagtgctaat
    23701 gatgagcgcg agagcacact taatcaatta ttagctgaga tggatggctt tagccctaat
    23761 gtaggtctca tagtaatggc tgctactaat agagcagatg ttttagataa agcactttta
    23821 cgtgcaggtc gatttgatag attgatttat gtcgatttgc ctgaactaaa agaaagggaa
    23881 gaaatcttta aagtacactt gcgtccttta aagttagctg atgatgtaga tgtgaaatta
    23941 ttagctcgcc aaacaccagg cttctcaggt gcagatatag caaatgtatg taatgaagca
    24001 gctctgatag ctgcgagaaa aaataaaaat gctgttgata tgtcagactt taatgatgcc
    24061 atagatcgca ttatcggtgg attagaaaaa cgtagtaaga taataacacc agaagagaaa
    24121 aaaattattg cttatcacga atctggtcac gctacagtaa gttggtttct gcaatatgcc
    24181 catccactcg tcaaagtatc tattgtccct cgaggcaaag cacttggggc cgcttggtat
    24241 ctgccaactg aaagaaatat tattacaaaa gaacaactac ttcatcagat ggcttcggct
    24301 cttggtggaa gagtttctga acaagtattt ttcggagatg taagtagtgg agctatgaat
    24361 gatctcgaaa ttgttactaa acaagcttat gcggcagtta cttattatgg attctccgat
    24421 gaaatcggta ctttgagctt ttataattca aatcctgatg aatttttcgg ttatcaaaaa
    24481 ccttatagtg aaaaaactgc tgaaaaaata gataaagaag ctaaaaaact tatagaagat
    24541 gcttataata ttgcttataa tattattaat gagcataaag atcagttaag tcaaatagca
    24601 cagcagttac tgaaaaaaga agttattttt agagaagatg tagaagcagt acttggagct
    24661 cgcccatggc ctgatcctga tgcagaatta ttttctgatc aagaaacaaa ttataacgag
    24721 gctaatacta atgaaaatta gtattaataa atatttttta taaattaatg atggcggtag
    24781 aagtagataa taaaaaaaag ttttcggatt tacctaaaag gtctttaact gcaattattt
    24841 atggtgtatt atggttaata ggtatttatt ttggtggatg ggtattagca ctagtaactt
    24901 atttattttt ctcaatcgct acttttgaac taataaaatt aataagtaca tctaaaagtg
    24961 aaaaaaatta tttttatact atctctataa gtgcaatcat tttattgatt atatctcaga
    25021 ttatcttgtt ttttaactat aaaaaattct catttttaaa aacttttttt attaatatac
    25081 catcattcaa atatttaatt ttattatcta taattataat ttttatcata ctaatattag
    25141 aaatcatata tattgtattt tataataaac aatcaataac aaactatttc aaatttttag
    25201 ctttttttat cttttggatt attataccta taatatctat acaattttta aatacattta
    25261 atcatttatt gataagtatt gtatctatat gggtatttga tacaatggca tatataatgg
    25321 gagtactggc tggcaaacgt ccattagcca aagacatctc tcctaaaaaa agtatagaag
    25381 gattattata tggagctttt ctcacttata taatttttgt aatttatttt agttttattg
    25441 agaaaagaac agacctatac tggatagtag ctatttctcc ttttttagca actataggag
    25501 acttattaga atctaagttt aaaagggaaa taggcgtaaa agatactgga aatatattgc
    25561 caggtcatgg tggtatgtta gatagactag atagtattat atttgccatt ccttttattg
    25621 ttatattaaa tattatagta attatatttt aaaaatatat gggcaaagga ttacatccaa
    25681 gtggttataa taacattatc ataggctcag taattatcat attgatagct attggattaa
    25741 tattatttcc ttttcattta ttgataaaag tattgatttt gatcacagct attattttga
    25801 taatttggat agcattattt ttcagaaaac ctaaaaaaac tgttaataat gctgctgata
    25861 atgaaattta ttcaccagct tttggcaaag tagtagtttt agaaaaaata tatgaagata
    25921 gatacttcaa tgaggagcgt attcagctat ctatttttat gagcatgtct aatatacacc
    25981 tgaattttat gccttgtaat ggcaaattac taaataaaga atatcaaaaa ggacacaact
    26041 atcatgctcg aaaagcaaaa gcctctatgc taaacgagat gtctacactt ttaatagaat
    26101 caaatactgg aaaacaaata ttaatgagac aaatagcagg agctgccgcg cgcagagtag
    26161 ttactttcat agacaaaaat gaatctgcta atgctggaga tacaattggc gtgattcgtt
    26221 tcggtagtcg cgtggacctt attttacctg ttgatacaga attatacatt aaagtaggtg
    26281 acaaagtaaa aggtggatgg acattaatag gtagatttaa ataaaattta ataatggata
    26341 atattaattg attttctttt ttttgataaa attagttatt tataattttg aatttataaa
    26401 aaaaatttta aatgatatta aaagctgaga atttagtaaa aatatatggc aaaaggacag
    26461 tagtaaaagg agtaacattt gatgtgaaac aaggtgaaat agtaggtcta ttaggtccta
    26521 atggtgctgg gaaaactact tcattttaca tgatagtggg cattataaaa cccaatgaag
    26581 gaaaagtatt tttagatgat ttagatatta ctagttatcc tatgtacaaa agagctcaac
    26641 taggaatagg ctatttagct caagaaccaa gtatatttag aaatttatca gttgaaaata
    26701 atattagagt agcattagaa tttaccaatc tatcacaaaa gcaacaagat gagaagctgg
    26761 agcaattatt aaatgaattt ggtctacaac acatcagaaa aagcaaaggc atacaactca
    26821 gtggtggcga gagacgaaga accgaaattg cgcgttcgct agctatagat ccaaaattta
    26881 tgcttttaga tgagcccttt gcaggcatag acccaatagc agtagaggat atacaatcta
    26941 ttatttataa tctaaaaaca aaaggtattg gtattttgat aactgatcat aatgtacacg
    27001 aaactctatc tatcacagac agagcttacc tattgataga tggttcaatt ctaaaaagtg
    27061 gatctgctga ggaactggca aatgatgaag aagttagaag attatatctg ggaactaatt
    27121 ttgaactacg caaatatttt gaatgtaata ataatgaata atagattttt aaattttact
    27181 atatgaaagc attagtatta ggctctggag gtcgtgaaca tgcaatatta tcaaaattac
    27241 agaatgaatt aggacaaaat aatactttta taatgcctgg caatggcggt acaaagccat
    27301 tttcgattaa tggtgatttg aaaaattttg aacatatcaa aaaaatagtt ttagataata
    27361 aaatcaatct tgtcatttgt ggcccagagc aaccactagc tgatggcata gctgattttt
    27421 ttgactctga tatacaatta aataaaatat tttttgtcgg tccaatacaa agagccgcta
    27481 gattagaatc atgtaaatct tttgccaaac aatttatgca aaaataccat atacctactg
    27541 ctaattttgc tagttttaat tataatcaat atgaagatgc tattgacttt ttgagtacta
    27601 tatcttctcc atttgtgatc aaagcatcag gactcgccgc aggcaaaggt gttatcatag
    27661 aaaacaatat caatgatgct aaacaaacta tcgaaaaact tttttttaaa aattacctag
    27721 gcgaagctgg taaagaaatt gtaattgaag agttcatcaa tggaatagaa atgagtgtgt
    27781 ttatagttac agatggtgat aattatattt tactgccaga agcaaaagat tataaaaaaa
    27841 taggcgaagg cgatactggc gctaatactg gtggtatggg agccgcatct cctactggtt
    27901 tttttaatgg aaatttaaaa aataaaataa taaataatat tattgaaccc actttgattg
    27961 gtttgaaaga ttcgaatatt gactataagg gttttttgtt ttttggttta atgataaatg
    28021 ctgatgatcc atacctactc gaatataatg tgagacttgg agaccctgaa acacaagttg
    28081 taatgccaaa aataaaatct tcattttcag atctttgtat atctattaat gaaaaaaaat
    28141 tgaataatta taaaatatta actgacgatt tacattattg ttgtatcact ttagctgcta
    28201 agggttatcc atttgattat gagaaaaata aattaatatt tttagataaa gaaaatataa
    28261 attctattat ctttcatggt ggtacaaaaa taaatgaaca gggacaatta gttacgtccg
    28321 gtggcagggt attatacgta gttgggtatg gagatactat tgattcggct cgtgatatgg
    28381 cttataaaga catcgaaagg attgattttg atggtattta ttatcgtaga gacataggta
    28441 tagatctatt aaagtagaaa ataatccttt atattactat aattaaacta tttttttcat
    28501 ttatgacaga ttatatttac taatttattt tcaataattt tttttgaaat aatactaata
    28561 acactaataa atccaaatat attataattt catcttaatt tttacaataa ttaagtttat
    28621 ttattataaa atttcattat ttagtaagct gtataacaaa tttttaaaag ttgtaattta
    28681 atgttatctt tatatgatag ggggtttaat ataattatat atttttttaa ttcaaaaaaa
    28741 atatataatt ttgcataaaa cataaaataa ttgtattatg agaaagcaca tttttaacgc
    28801 aggtccagca gttttaccag taccagtatt agaaaaggta tcaaaatcag ttcaagattt
    28861 agatggtata ggtatgtcaa tacttgaaat ttcgcacagg tcaaaggaat tcgaggctat
    28921 tatcaatgac gctattaatt taatgaaaga aatcatgcaa atacctgagg ggtatcatat
    28981 tatttttgta ggtggagggg catctactca gtttggtatg gtagctatga attttttgaa
    29041 taaaaaagca gcatacctag agacaggtaa ttgggcaaaa aaagctatta aagaagctaa
    29101 attattcggt gaagtacaaa tagtagctac atcagctgat aaaaatttca cttatctacc
    29161 aaaaaacttt attattccta cagatgtaga ttatttccat attactacta ataatactat
    29221 ttatggtact gaattaaaga ctgatttaaa ttcaccagtt ccattagtag cagacatgtc
    29281 atcagatatt tgcagtcgtc ctatagacat ttctaaatat tctctaattt atgctggagc
    29341 tcaaaaaaac ataggtccag ctggagtaac tattgcaata attaaagatg agttcctaaa
    29401 tcatcaagtt actgatagga taattcctac gatgctcaac tacaaaactc atacagaaaa
    29461 taatagttta tacaatactc cgccagtcta tgctatttat gtagtcaaag aagtaatgaa
    29521 ttggttaaaa aatgaaattg gtgggttaga aaacatgaag aaaattaatg aagaaaaagc
    29581 aaaaatttta aatgattact tagataatag taaaatgttt gttcctaccg tacctgataa
    29641 agaagatcgt tctctaatga atattacctt tgtaatgcaa gaaccctata aggaactaga
    29701 agctacattc atagattatg ctaaaaccca tggtatagta ggtattaaag gtcatagaag
    29761 tgtaggtggc tttagagcat ctatgtataa tgcattacct gtagacagtg ttaaagcact
    29821 tgtacaagct atggaagact tcgaaaaaac taatttgaaa taatttttca ttagtttata
    29881 ataaaatgtt taatttttta aaaaaattat aattatgaat aaaatagtag ttttaacaga
    29941 aaagcctttt gcagctgacg cagtcaacaa gatgaccgaa attgcaaaag ctgaaaattt
    30001 tgagattaaa tttatagaaa aatatacaag taaagaagaa gcttataaag gtgtaaaaga
    30061 agcaaatgct cttatagtac gtagcgatat agtagataaa gatctaatct cccatgctcc
    30121 aaatctaaaa atagtagtaa gagctggagc tggctatgat aatatagatc taaatgcttg
    30181 cactgaaaga aatatagtag ctatgaatac acctggacaa aattctaatg ctgtggcaga
    30241 actagcatta gcattaatgc tttatatttc acgaaatcaa tttgatggtt ctgtaggtac
    30301 tgaactaaaa ggaaagacct taggattaca tggttttggc tatacagcta aagcacttgc
    30361 tacccttgtt aaaggctttg atatgaaaat ttatggtcat agaagatcta aaagagatga
    30421 cgaatacatt gcttataatg ttacaaaagc tgagtcagta gaggatttat ataaaaattc
    30481 tcaatatgtt tctttacata ttcctgctaa tgatgctacc attaaatcta tcaattataa
    30541 cttattatca ttaatgccta aaaatgctgt cttagtaaat acagctagaa aagaacttat
    30601 taatgaagat gatttattaa aagttttgga agaaagacca gattttcgct atgtttctga
    30661 tgttgcacct gattgtaaaa atattattgc agagaaatat cctggacgat gttatttcac
    30721 tcctaaaaaa ttaggtgctc aaactaaaga agctaataat aatgcaggat tagcagctat
    30781 aagacaaata attgcatttt ttaaaacagg agatactact tttcagataa ataaataaaa
    30841 atttaaaaaa aaataatcac ataaccaaaa aacatacaac tatggaagaa aatagaccta
    30901 tgaacttctt cagatttgaa gatctacgca tttatcacaa aactttagat tttgtatctt
    30961 gggtttataa tgctacaaaa ccatttccag actatgcatc cattcttaga aatgacatgg
    31021 tggaggctgc tcaagagatc actataaaca tagcagaagg atcaggtaga aataaaaatc
    31081 aattcattta ctacctaaaa atggcgcgta gttctattag aaaaagtgtg gtacttatta
    31141 ctatcgctca aaaagaaggc tttattactg atgatcaaca tgaagaagca aaaaacttat
    31201 taattgagat atctaaaatg cttggagctc tcatttcttc attacagcgt aacaaaaaaa
    31261 ctgatagaag tgcctctgaa gaagtagaag aagaagagga ggaggaggat tttgatgaaa
    31321 atactggcaa cgaactaata taatcttgta gaatactcgt tatttatcct atttttatac
    31381 aaaaaaataa gataaaagtg agttgatttt tttaagttag ctaaactaaa ttttactttg
    31441 gtttggttat agtttaatat ttattttaat ttataaaaaa aattataatt atggcaattt
    31501 ttagaccttt caaggcatta cgcccaccaa aagaattagc accaaaagta gctgctaaac
    31561 cttatgatgt tattaatact gaagaagcaa gaaaggaagc agaagggaat ccatattctt
    31621 tacttcatat aactagagca gaaatagatt tacccgttgg tactgatgaa cacgatccta
    31681 gagtttatga aaaagcaaaa gaaaattttc aaaaatttat agaaaataaa tggttaatac
    31741 ccgatgatga tgcatatttt tatatttatg ctcaaactat gtggaacaaa actcaatatg
    31801 gcatagtagg ctgtgctagt atagatgact attttaataa tgttataaaa aagcatgaac
    31861 tcactcgtag agataaagaa gaggatagga tgaaacatgt gagagttacc aatgctaatg
    31921 tagaacccgt attttttgct tataaatctg tagcagaact tgatagcttg ataatgaatt
    31981 atgctaatga gcacaaaccc gaatatgatt tcatagctaa tgatggtgtt ggacatcaat
    32041 tttgggtaat aaaagaaaaa gaattaatag ataaaataca aaaattattt gcagaaatgc
    32101 cagctttata cgtggctgat ggacatcacc gtacagcagc agcagcatta gtaggtaaag
    32161 aaaaaagaga acagcatcct aactatacag gcgaagaaga ctttaactat tttatggctg
    32221 tcgtttttcc agataatcaa cttacaataa ttgattataa cagagtagta aaagatttga
    32281 atggtttgac accacaagaa tttttagata aattatctga atattttgat atagaaaata
    32341 agggcaaaaa tgaatacaaa ccaaataaac ttcacaattt ttctatgtat ctagatggga
    32401 attggtattc attgacagct aagaaaggca cttataacga taatgatcct attggagtac
    32461 tagacgtaac tatcttatca gatttagttt taaataatat attaggaata aaggatctga
    32521 gaactgataa aagaattgac tttgtaggtg gtattagggg gttaggagag ttaaaacgta
    32581 gagttgacag cggcgagatg gctgtagctt ttgccttata tccagtttct atgaaacaac
    32641 tcatggatat agctgatagt ggtaatataa tgcctcctaa aactacatgg tttgaaccta
    32701 aacttagaag tggactttct atacatttat tagattaata aattaagttc aattatttta
    32761 ttataaatac tgtatttcag ttaaatttag gtggttataa ttttttgaaa tataatttta
    32821 tttcactttt gaataggtat attaatatat attttacgaa ctatataagt taattgcttt
    32881 taaataaaga tttgttttag taattaataa agtgtataca ataccattat tatgcataaa
    32941 atatataata atggtattgt atttggcttc tattaaaatt attttttata gtgacaaaaa
    33001 cgtatgatta taatatcttt tcattatttt taattatcga atctgaaaaa aaatgtgtat
    33061 atgaaaattt taggagagtg gggtgaatta ataaatctaa tatatgtttc aatcaacata
    33121 tgttagattt attaactcct tataccgata ccatgcccac ctgtccgagg atgcgaggtt
    33181 gagcatttgc ccagtattgc ttacagtctt gcctgtgagg tatttaccca tcactacata
    33241 ccacataatg tagttttgca gatatttggt agctacgccc ctaaatttga aatatatcca
    33301 attagtgaat tcactag
//
LOCUS       DAOPRN010000017        32349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1071_length_32349_cov_137.257230, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000017 DAOPRN010000000
VERSION     DAOPRN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32349
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1071_length_32349_cov_137.257230"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PKI65_03260"
     CDS             complement(<1..247)
                     /locus_tag="PKI65_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77494.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMNKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFR"
     gene            complement(515..2653)
                     /gene="scpA"
                     /locus_tag="PKI65_03265"
     CDS             complement(515..2653)
                     /gene="scpA"
                     /locus_tag="PKI65_03265"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOB77495.1"
                     /translation="MKPDFKKIKIFEDNNPQLKIIANVVYDSTEGIPIKNFYTYEDIV
                     ELEHLDYAAGIPPFLRGPYATMYTQKPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLS
                     VAFDLPTHRGYDSDNERVLGDVGKAGVAIDTVEDMKILFDQIPLAEMSVSMTMNGAVI
                     PIMAFYVVTALEQGAKLEELKGTIQNDILKEFMVRNTYIYPPEQSMRIIADIFKFTSQ
                     YMPKFNSISISGYHMQEAGATADQELAYTLADGLEYLRTGLKAGIPIDKFAPRLSFFW
                     AAGMNHFMEIAKMRAARLLWAKIVKQFNPQNPKSMSLRTHTQTSGWSLTAQDPFNNVA
                     RTCIEAMAAAFGHTQSLHTNALDEAIALPTDFSARIARNTQILLQEETQICRVIDPWG
                     GAYYLETLTDLLAHKAWALIQEVEELGGMTKAIIAGIPKMRIEEASARRQARIDSGLD
                     TIVGVNKYKLEKEEPIQILEVDNTIVREKQIQRINEVKASRDNKKVQQALDAITKACE
                     TGEGNLLELAIEAAKHRATLGEISYAMEKAWGRYTPEIKSITGVYSSEISQNENFRKA
                     QQLADEFAKLEGRRPRILVAKLGQDGHDRGAKVVATAYADIGFDVDVGPLFQTPVEAA
                     KMAVENDVHIIGISSLAGAHKALVPQVIEELKKLGRDDIMVIVGGVIPPQDYQFLFDA
                     GVVAIFGPGTPIPDAAIQMLEILIASRKVN"
     gene            complement(2675..4534)
                     /locus_tag="PKI65_03270"
     CDS             complement(2675..4534)
                     /locus_tag="PKI65_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOB77496.1"
                     /translation="MPKEKLFAEFPPVSPEEWENAIKDSLKGADILKIIWKTDEGFEV
                     KPFYTKNDIEDLLELTYYPNVYPYLRGYNDKKLKWQVIQNIDAKDLKCANEAALKALS
                     NGADAVEFDIQNIDKIDYLNILLQDIDIAKYSLHFNDLHSYYSLAQLIKVWANEHHLN
                     INQLKGSFNFDPFGFYLLNGEYYNSYKDDINGLLNLFQFVSDWNPEFKIININGIIYH
                     NAGANIIQEIAFSLAQLVEYFDILTDNDIEIEKIIPRIRITFGIGSTYFLEIAKLRAT
                     RMLIAKILEAYGIPNENAKITIHSKTGIFNKTAYDAYNNMLRNTIEAMAAVIGGANEI
                     TILPHDITLNHSNDFSRRIARNVQIILREEAHFDKVIDPAGGSYLFENLSASISQHSW
                     NLFNEINDLGGFRKLMDKGWIKNEVDKSAKQKQDAVLKRKLNIVGVNNYPNFNEQISS
                     QLKKQLISESKNEKALRIFRIAEPFENLRFCTEQHVLKGNKKPLVYLLPFGDIAMRNA
                     RANFSRNFFGVAGYDIMDAEPYDNDEEMLKLIKQHNPDIIVLCSSDQEYGTTGLNILN
                     TINNAFPNVNVVIAGNPTEYIEILKEKGVNEFIHIKSNILETLESFHKKLKIM"
     gene            complement(4626..5636)
                     /locus_tag="PKI65_03275"
     CDS             complement(4626..5636)
                     /locus_tag="PKI65_03275"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77497.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEIVTSNRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNITYVNLN
                     IY"
     gene            complement(5809..7038)
                     /locus_tag="PKI65_03280"
     CDS             complement(5809..7038)
                     /locus_tag="PKI65_03280"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOB77498.1"
                     /translation="MREKKLLEGKTVGILTGGGDTPAVNSSIEQIRNHVAILGAKIYG
                     IRKGWKGLLGDGDIVDLTNQPFDGKCGGSALKSSRTNPFPSKKNPESRVDQVLKNLDR
                     YSIDILVTIGGDDTNGAAKKLWEQEKIPVIGFPKTIDNDLHTQTIHNYKGKEIEVCLC
                     PGFPSAANSVVEFTQKIRTTAASHNRVMVLEVMGRNAGWLTAAASYGFADMVLIPEYE
                     ITKERKEEFFECIKKKYQKDNYLIIAVSEGTRWYNDQKDQVEVVYASTETDEYGHPRL
                     GGISGVIASEISKKLKIDARAQIIGYYARSGKCYEYDRKLTVALADKMLDLLLREDFG
                     KMPVMKKIVSYPYVEEYNTTAIEMGDVDNLPLPLDYYNPLEFQMAESYFDFLYHILGH
                     IEKYEFKYNYPKVYPIK"
     gene            complement(7035..8423)
                     /gene="hisS"
                     /locus_tag="PKI65_03285"
     CDS             complement(7035..8423)
                     /gene="hisS"
                     /locus_tag="PKI65_03285"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOB77499.1"
                     /translation="MSIIKPSIPSGTRDFGPDQVKKRNYIIDILKKIFELHNFLPIET
                     PAMENLSTLLGKYGDEGDRLIYKILNSGDFLNNLPDHLWKTKDINSWIEYLSDKGLRY
                     DLTVPLARYVVMHRNEINFPFKRYQIQPVWRADRPQKGRYREFYQCDIDIIGTDSLLA
                     EIELVTLADEVFTQLNLSTTTKINNRKILQGIAQWLCVDAEGFIPFTITLDKLDKIGL
                     EDVLSSFKEAGWSDNNITKLKNLINFSSTNKDWSEVEIYLNKILGDSEVGLAGLDEIS
                     FIMRNLDFINLNNNLIFDITLARGLNYYTGTIFEIISNEMSIGSICGGGRYDDLTALF
                     GLQGLSGVGISFGAERIYDILEKLNRFPDFITREDKILIVNFGENYLKYSLPILLDLH
                     KAGFIADIYPDEVKLKKQFNYANELKFKYVIIIGEDEIANDEVSIKDMYSGEQVKISK
                     NNIISYLKNKEL"
     gene            complement(8554..9465)
                     /gene="rsgA"
                     /locus_tag="PKI65_03290"
     CDS             complement(8554..9465)
                     /gene="rsgA"
                     /locus_tag="PKI65_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOB77500.1"
                     /translation="MKGLVTKSTGSWYYVKSIETGEIIPCRVRGKFRLQNYKTTNPVA
                     VGDYVEIELEQSKTEPSGIITFIYERENAILRKATKAGATYQIIATNLDGALLMVTPQ
                     HPFTPMGFIDRFLVLAEAYNVETTILFHKCDLYDEKDEYFQLLYNIYHQIGYKIFLTS
                     IITKQGIEELKNFIKNKTFLLVGQSGVGKSSLLNTMDRSLNLKTQELSAKYLKGKHTT
                     TFAEMHFLRSGGAIIDTPGIKELGLPNFQKWELGYWFREFIPYIKNCKYNNCTHINEP
                     DCAVIAAVKNGDISTQRYNNYLNILLD"
     gene            complement(9469..10158)
                     /locus_tag="PKI65_03295"
     CDS             complement(9469..10158)
                     /locus_tag="PKI65_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOB77501.1"
                     /translation="MDKFVTSFFNILFPKTCVSCENPIYDKNNILCSDCFSKLGFTYY
                     KNFVENPLYEKFIGQANIEYAYALCYFYKDGILQHCLHALKYKGHKDVGTFFGKLIGY
                     ELNDIDPPVDIIIPVPLHPKKEKVRGYNQAEMIAKGVSKVLKIPIAINIIKRTKFTET
                     QTKKTKWERYDNLNGVFAIQNSKSLANKHVLIVDDVITTGATTVSLIDLLKDIENIKI
                     SVACIGTPDKI"
     gene            complement(10187..11128)
                     /locus_tag="PKI65_03300"
     CDS             complement(10187..11128)
                     /locus_tag="PKI65_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77502.1"
                     /translation="MNDISNMGNDKFEIDNAPKEKKKNTWYIILIILLLVTNAIFIYL
                     YFSSQNKFQTAVVEKERTLQETYQLKYELDSIMREYDKIKEEYGILNIKLSDQDSLIK
                     EQAKEIEKLIASQADYTRIKRQLDRLRNITQSYVRQIDSLYRINQELTVENVKLKEDL
                     TYEKTKAIELSTITDSLSKTISEATYMVAYNITPIAYKIRGNKATPTDKAKRTDKFDI
                     CFTIGENKLVPTGEYTLYIRIARPDGLILTQGGYSFDYQGRNLQFSEKTIINYTGKAQ
                     KVCVDYARDNLNLIPGIYYFSVFTNDYILGEGSLKLE"
     gene            11259..11615
                     /locus_tag="PKI65_03305"
     CDS             11259..11615
                     /locus_tag="PKI65_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77503.1"
                     /translation="MKKTILLLTIGLVFFSSCKKNVAPIVIKKNITYKVNSTAERLSV
                     NYYNEHGVMVASEIVGKTWQYDFKATPNTYLYLGAKNLTPTGSVNIEILVNDKVKFKN
                     STVLPYGAVNCSGFVD"
     gene            complement(11620..14208)
                     /locus_tag="PKI65_03310"
     CDS             complement(11620..14208)
                     /locus_tag="PKI65_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOB77504.1"
                     /translation="MENDYHSLDFYKSTLSLNSLSLVVTYLNYFVFDKLSPMIINLFF
                     QNIFINENYTLICGHEKNDFFYSKNIPINNVNIKINDLDNSFEIIKQNNEDYFVEINI
                     KNLYCHDSSDINKNKYWYFAYLNCANQMLIGFEKPISNEKYLFLNKYLYIVNHIKNTI
                     YKKDSSSKSLNTCQLSILITNKDNQLLLSDPFSLQFFDISQYLSFYNKFLSFERNDLH
                     IEKKLNICIKEIHISNIPDHILQKNYSTVISFINTYTFSTNDITEPFTYHIIGLDLQN
                     AIKKYSDNINNYKNIIENIQSVYYEILDNVIIEISPSVYDFLGYKREELKGKPAVEFF
                     YSAKEREDYINKLRDKKQLKNQLLKLINKDNQLVYCLTNSKMVIENGKEKIIGSLTDI
                     TEMIKSNKELEKKNIELENLINNDLIGVIYATTDGEVKLCNELAKQYILIDENNCLLT
                     QCNTFKQIINFDELLSTLNNTGKFSKIATITRDNQRYDFDIQASLINYPNSDDKRLLI
                     FITDITEKLQINNELEQINLNFNEVIENIEEPLFIYDQTGTLLYANPKFYKYLNYQES
                     ELLNNNISKFTPKDTYDLLINIFKNIKKLKNKKTELSLISKSGKPQLYEAVFSCRYSY
                     RSTSNVAVVLLHNIEERKMYEENLLKNINEKDKLIKEIHHRTKNNLQLILSLIKLNLQ
                     KNININNLNAITALRDIYEKVSLLSYLQNELYFAKNSNVADIKAVIVKETNRLIKDFN
                     KKIQLYLDLPDEIIMHVDVAIPFMLSYIELIYNSIIHAFPIDWENPTITVKIEKVNNG
                     INLEIIDNGIGISALDIQNFATTLGLSLVNTLITYQLGGTFDITPLSSTGTRASISLK
                     NILLKK"
     gene            14251..15534
                     /locus_tag="PKI65_03315"
     CDS             14251..15534
                     /locus_tag="PKI65_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOB77505.1"
                     /translation="MSLILKNANLFKNKFNEIWVGNVLIDKNSNKKVIYSKEISELLD
                     KSSEATIIDCNRLLVMPAYINAHFHSQNFFSQLILTDETILGLQKQEKIYHKLDKIWD
                     RDLLEAAALYSAINLLKNGTIHVIDMLSGPAIASIDNIQIIYETYKKVGVNVMPVIVI
                     TDKYGFGVAENLLDTTEAWLKSHKGLMGLDHLFNVGTGTLTEVKRLMDKFKCGIHGHL
                     AEYAYDQSHSIDKYKLSVVERLDALNLINNPRNMWAGCLHITNDEVDLIRENKNNVIE
                     LYESSQWENKGYFSSEGLPLIMFGTDGMHSDMWQTMKNTYYFNTEYDRISINDALKRL
                     YQPHKYFSRFFPSIYDPASFVIYDCPLINCINEEMKPMQLLQLIDRKYIKYVISQGEI
                     TVEDNKLTKIKELDAYILINRQFKRLKSLYNNRQY"
     gene            complement(15597..16232)
                     /locus_tag="PKI65_03320"
     CDS             complement(15597..16232)
                     /locus_tag="PKI65_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77506.1"
                     /translation="MKKNIFVLLIALILLAGCDKEPKEHSFTGMVKDQFLDKPVANVK
                     LDLQAQVLQDNIFSVAYQTISTGYTDDLGRFQLNYGDNVYDEYRLILNKDGYFYKTIN
                     LGNKLINDKIYTIAAKGVIKIHIKNINPYDENDFISISWIIPNDAYFNSEINFPFIVN
                     GLDVDLFLVCNVNAYAPYILVANWKHNAIEIIRSDTITPLFNDTTYYDLFY"
     gene            complement(16278..17615)
                     /gene="murD"
                     /locus_tag="PKI65_03325"
     CDS             complement(16278..17615)
                     /gene="murD"
                     /locus_tag="PKI65_03325"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOB77507.1"
                     /translation="MNPIIEEFINKNICIWGWGREGQSTYSFINKFLPNANITIADKN
                     KIKEKSLKYISETELIEKIDLFDLIIKSPGISLYNFNIKKSDKLTSQVELFLKHYKHK
                     TIGVTGTKGKSTTTSLIYHLLRSINIDAKIGGNMGIPVFELLINDADADWYVIELSCH
                     QLDQIKLSPHISILLNIFPEHLDYYKSFELYKEAKLNILSHQNIHDIAIINNSMREIQ
                     MINSEIWQFGNYDINENRGLFYQTKNNFIFKNKNFNKKLIIHEDKLQLKGKHNLLNIS
                     AALLASYLLSNFDIDKLIDYLYDFQPLPHRLEYVGKYQNIDFYNDSISTIPQSTIAAL
                     ESLGNVDTLILGGMDRGIDYTELIDYLKKSKVRNIFCIGELKIFLYNNLKENQDKNIM
                     IFDSLEQAIDAAYKYTLPDKICLLSPAAPSYDQFKNFEDRGDKFKTFVKIKGK"
     gene            complement(18052..18444)
                     /locus_tag="PKI65_03330"
     CDS             complement(18052..18444)
                     /locus_tag="PKI65_03330"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOB77508.1"
                     /translation="MNIEETKNWIKKAENDLKVVKYLIEGKDPITDVICFHAQQCVEK
                     YLKAFLVYNNKSFHKTHNIAEILRLCIEIDTEFEELAKIDVHELTVYATELRYPEFFY
                     IPSIDEAKKCLELALKVQEFVVKKLNIK"
     gene            complement(18441..18773)
                     /locus_tag="PKI65_03335"
     CDS             complement(18441..18773)
                     /locus_tag="PKI65_03335"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOB77509.1"
                     /translation="MEDDSLDLTEVKKIITEECEKAGFNVVKIILFGSRVRGDYTEES
                     DWDLLVILDKDITFNELKKLTGRIQLKLAAINFSNDLIFRGINQFNKSMKVVGNISYY
                     ANKEGIEI"
     gene            complement(19483..20043)
                     /locus_tag="PKI65_03340"
     CDS             complement(19483..20043)
                     /locus_tag="PKI65_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOB77510.1"
                     /translation="MINREYALDLLNKHVSNPKLIAHSLASEAIMKKLANYLGEDEDK
                     WALAGLLHDIDVELTNGVPTLHGLKAKEILQKEKFDNDIIEAIVLHNEIAAQSLRSKH
                     FHHALAAAETLSGFIFAVALIYPSKKISDVKVQSVVKRMKEKRFAANVNRDTIMECEL
                     IGIPLNDFVKLALEALAPIEDELAYK"
     gene            complement(20055..20522)
                     /locus_tag="PKI65_03345"
     CDS             complement(20055..20522)
                     /locus_tag="PKI65_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOB77511.1"
                     /translation="MSTIEVIVVILLVLAAIRGIMTGFLRQLVIFVGYYLLVLLLFGI
                     IGKNAFAMQLIPQNSSIFQTILTMIVLIIGGALLVKFAAKIINAIFNKIPVLGTANRV
                     LGMIFCVILVMFIFGSFISILLNLGVKVESLGLDKRGISHICYKIFTTLVNIF"
     gene            complement(20519..21271)
                     /locus_tag="PKI65_03350"
     CDS             complement(20519..21271)
                     /locus_tag="PKI65_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOB77512.1"
                     /translation="MIRVEHLTKKFDDKVVLKDITTRFEPGKVNLVIGMSGSGKTVFM
                     KCLLGLLIPEKGELYYGDKNFYSLSEKEQKLIRKEMGVVFQSGAIFDFLDVEGNVKFP
                     LDMFTNMSNKEKLERVNFCLERVNLQGTNKLKPPELSGGMKKRLAIARAIALNPKYLF
                     CDEPNSGLDPQNAVLIDELISDLTHEFNTTTVVNTHDMNSVIKIGEKVIFFYNGMLWW
                     EGSKDNIFETDNQELKDFIFASSLAQKLLESL"
     gene            complement(21278..22018)
                     /locus_tag="PKI65_03355"
     CDS             complement(21278..22018)
                     /locus_tag="PKI65_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOB77513.1"
                     /translation="MNLFEGIADYLLVMKKTFTRPVKFKTLANQVVHEMYYLGVESLP
                     IVCFLSLFIGIVVIIQTAFQIESPLIPKYTAGFITRVVIIYEFSPTIMSMLLAGRVGS
                     RIAAEIGTMKVTEQIDALEVMGINSASYLILPKIIAFVIIMPVIITFSIFSGLFGGYL
                     VTIGSTQIPPSEYFYGLRAFFTPFEITYALSKSLFFGFIITSISGFYGYIVEGGALEV
                     GKNSTKAMVQSSIFIIIFDVILTQIFLG"
     gene            complement(22047..23276)
                     /locus_tag="PKI65_03360"
     CDS             complement(22047..23276)
                     /locus_tag="PKI65_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOB77514.1"
                     /translation="MNRFGHIKYSKFIFIIILLICIPLLSQTEAQKKKLEQERANLEK
                     QIKNTEKLLQSTRKNRKNSLAELDLLNAKLRDNQKMRNIITSELAYANTKLSEISKEI
                     PILQQNIQDLSVGLSKIITLMYVYKTQQNRISFVLSANSFNEAFERYQYLKQLANIYK
                     LQIKNYQASLQKLMDLQQEYEQLKEEKQKLLAQQDSILIVIEKEIKNKNILVNELKKK
                     ETNLEKQIKEQKKVQQELEKQIQIIIKELERARAAKAKEASKSPSGAPATKASPSIAS
                     IYRGKLSKPVDNGVIVGTFGNQSHPVYQNITIQNNGIDYNVKEDEWVKCVYDGTVSAI
                     FSLPNQLKAVIVKHEDFLTVYSNLSTVNVKQNQKVNKGTIIGKTGFLKDYKANILHFE
                     IWTDSKEPENPLLWLSN"
     gene            complement(23545..24711)
                     /locus_tag="PKI65_03365"
     CDS             complement(23545..24711)
                     /locus_tag="PKI65_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOB77515.1"
                     /translation="MSKNILPKEALEKRSYKKDEDIRGCYYRDQTAIIHSRPFRRLKH
                     KTQVFFSPDDDHVCTRIEHVLHVATISATICRGLNSKDNSEWSLNEDLAYSIGLGHDL
                     GHTPFGHAGESELSFLLGGKDLFIHEINSYRVVEKLTNQGEGLNLTYAVKDGIIFHNG
                     ESFEKSIKPDQTIKNLDTITDRKHLPTTYEGCIVRFSDKIAYLGRDIEDAIIAGFFSK
                     EDIPDIIKNKIGTSNGEIINHLVLDVIDNSTDDEISFSDECYELMLKLKDFNYKYIYD
                     HPRLKEYKIYCKRIIETLFDYLTKLFCQYHWEIDWYLKENNLNIDIFFGNYLKGMKNF
                     YINEKQQNNLTEGDLCKQIVTDYISGMTDKFAIDCMKEISIPRPIEFIQRPYSL"
     gene            complement(24929..26221)
                     /locus_tag="PKI65_03370"
     CDS             complement(24929..26221)
                     /locus_tag="PKI65_03370"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOB77516.1"
                     /translation="MKFLEAEKFIMEQLPMYERQGSSGYQGGIEKTLFMLNKFDKPHL
                     KIPMIHIAGTNGKGSVSSLIASILQEAGYKVGLYTSPHLKTMRERIKVNGEMIPAQWI
                     AKFTTKNFKLLEQLKPSFFEINTVMAFSYFAQSNVDIAVIETGLGGRLDATNVIIPLV
                     SIITNISYDHTNLLGNTLQEIAAEKAGIIKNGIPCIIGENDQETLPVFIDFAYKNNAR
                     LIISADEWKLIDFSQNLLPPSMRLHLKNNYNDDFFDIYSPLIGKFQIDNIKTVLSAIN
                     ILQTYKYIISTKHIINGIKNVFDNTGFFGRWTYVPDRIPIIMDIGHNVNAVSNVIKIL
                     ESIPKDHLHIVWGMMADKDIDGILSLLPKDAIYYFCSPKVPRALDFMLLNEKAKNFNL
                     SGKTYNSVSNALNAAKSQAIANDIIYVGGSAFVVAEAM"
     gene            complement(26272..27243)
                     /locus_tag="PKI65_03375"
     CDS             complement(26272..27243)
                     /locus_tag="PKI65_03375"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOB77517.1"
                     /translation="MFFEIKQSINRFYNLILFAFSYWYSKLFGKFCLLNLPFAIHIET
                     CSLCNLSCLFCEQGLGNINRADSFIAFTDFQRLINNLPKNIFWITLYFQGEPLLDDTI
                     IEKIKYLRSNDFYVELSTNAQNISYNVATSLVNSGLNRIVVSMDGISQKTYEKYRTGG
                     NIDKVYDAINNLIKAKHILKKKSPKIIVQFVVFRHNEHEIRLFKKHMKKIKVKTSIKS
                     AQLVENAIDFLPKNKRFSRYKILNDQLILKKLPSTPCTRLWTELILLQNGQIAICCQD
                     KNANFLNDNWYKKSINEIWFSEELNKIRIQSIKGKYLDICYNCDKGF"
     gene            complement(27383..28531)
                     /locus_tag="PKI65_03380"
     CDS             complement(27383..28531)
                     /locus_tag="PKI65_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOB77518.1"
                     /translation="MNNKNNVQHFWGNIFKKWYVWLPSLILIALIFILLPKVLLYLLI
                     SFVISLILAPIKNLLLKIRIKKFHLNDAVATIISMLFLLGVIILFFFYVLPPIITKLT
                     SLIAIDYDILLLQLESSLKPIEKTLIDYKIANESFSFAQIIQKYITNFQASINIEKAF
                     ENIINSFGDIVVGVVTVVFFSFFIIKDQNVFPSLVKSLFAERYHSMIDNIINDTRVLL
                     SRYFLGILIDQIIVFILIFLVFTLIGYDYAILIAFFAGLFNIIPYFGPLIALILAILL
                     GIVSSLMSGADTQLLWNIVITIIGFESVKILDNLVLQPNIYAKSVKSTPFEVFLVLVI
                     GEELFGIIGMILSVPVYTLLRIIAVQTLQGVPLIQKITSDLEKSIEKK"
     gene            complement(28538..28978)
                     /locus_tag="PKI65_03385"
     CDS             complement(28538..28978)
                     /locus_tag="PKI65_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOB77519.1"
                     /translation="MLKKKKYCYDYPRPAVATDIILIHKQSVLLVKRANEPYKGMWSL
                     PGGFNEENETLEDTARRELKEETSLDICFLEQFKAFSDPKRDPRTRVITIVFYAILPD
                     DFLLSRTQAGDDAAEAKWFLLSLLPPLAFDHKLVLDEFIKFKYL"
     gene            29064..29846
                     /locus_tag="PKI65_03390"
     CDS             29064..29846
                     /locus_tag="PKI65_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOB77520.1"
                     /translation="MRKIYLFIILFITVFNGKAQTPLTIAVDFTTSDVNGNVHNLFNY
                     LDNNKYVLLDFFFTRCSACQTSSPQCNASYIYFGCNQGDVIFLGIDYYDSRQDVINFI
                     NQYGLDYPCISGDDGGRQICEMYGIDAFPTYILIAPNKNIVERDIWPFSTNICNSILQ
                     SYGLQTMSCQENINDFYDENISIYPIPAKDEILLGFYNYYLNEPIIFIYDLTGKMIYT
                     EIITNLSNTHKMDISAIDNGTYILLIVENNEVLLRKLISVSH"
     gene            complement(29891..31066)
                     /locus_tag="PKI65_03395"
     CDS             complement(29891..31066)
                     /locus_tag="PKI65_03395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOB77521.1"
                     /translation="MNKLIIRTGKVESIKRYHPWIFSGAVKKIEGEPKEGEWVEVYDN
                     HNNFLAMAQYSTKGSIIGRIVSYQKENRSVEFYKNKIMRAVAIREAINLPLSNITNAF
                     RLVYGEADGLPGLIIDFYDYHLVLETHSTGMYNDINYIIETLKQIYREKLKSIFVIDK
                     TQNIKNTYYAYQTDNIEIPVIIKENNISFYVNWEEGQKTGFYLDQRENRKLLQRYVKD
                     KNVLNMFSYTGAFSVYAALADATKVVSVDSSREAINLLYKNFELNKINKTRYEAIEDE
                     AMHYLNNMQENYYDVIILDPPAFAKSINTRHNALKGYKRINSKAIEKIKNNGILFTFS
                     CSQAVSSLHFEGSVMNAALDSGRNIKILHRLTQAPCHAPSLYHPEGYYLKGLVLLIY"
     gene            complement(31126..32184)
                     /locus_tag="PKI65_03400"
     CDS             complement(31126..32184)
                     /locus_tag="PKI65_03400"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOB77522.1"
                     /translation="MKKLTFIIFIFICSNTFCQKYILVLDAGHGGKDPGAVYGNIQEK
                     DITLSLVLEVGNILSNTYENIKVIYTRQSDIFIPLIERTRIANKSHANLFVSFHVNAS
                     KSIEANGYEVFVMGNTKVKAWLETAIAENSVAIYEENYLESYDGIDPNSIEGYIAFSL
                     YQNQFLNKSISLADKVTNSTLQIAPFTNRGIKQAPFFVLYKATMPSILIEMGFISNQH
                     DREWITNKDNQKKLAQNLANAIADYIVNVDNFPLKEKSILATQNQLSKANDETNEELI
                     YYIQFLSSNEIFTNTHKIYKDFPSDSISYYFFNNTYKYIYGEFTDYNQARQKLNYVKD
                     KGYNDAFIVKFRNGKRVL"
BASE COUNT        10874 a         4898 c         4264 g        12313 t
ORIGIN      
        1 ctctaaactg tctgccgcag tctttgcagc gatagttttg catcccattt tgtaaaccat
       61 ttttaataat gtgattgcta cgacagtctg ggcatagtgg tggggcactg agtattttca
      121 gaccttgata atcgactaca tcggcttcta atttattcat taatgaataa aggttgtcta
      181 agaattcttt attctcttcc ttactcatag caagatattt actggtattc tctacgattt
      241 ttttcatagc ctaaaatttt tgtaaaaata taaaaattat ttaacatata aacttgaaaa
      301 acttaaacat ttattaattt atttattatt gggatgagtg gaactttgta taatatcttt
      361 gtgccctttg tggtaaaaaa acaacctatt tgtaacggat ctaaaatgtt tttaccaaca
      421 ataaacgtta tagaatcaaa ataaattttt gctatgtcaa attattatag tgagatatta
      481 attaaaataa acatcaaaaa gtgtattaga aaaattagtt tacttttcta gaggctatta
      541 atatttctaa catttggata gctgcatcgg gtataggagt ccccggaccg aagatagcga
      601 ctacgcctgc atcaaataaa aattgataat cttgaggggg aataacgccc cctactatta
      661 ccattatatc atcacgtcct aattttttta gttcttctat cacttgtggt actagagctt
      721 tatgagcacc agctaagcta gaaataccaa taatatgtac atcattttct actgccatct
      781 tagcagcttc tacaggagtt tggaatagag gtcccacatc tacatcaaat ccaatatcag
      841 cataagctgt agcgacaact ttagcacctc tatcatgtcc atcttgtcct aatttagcta
      901 ctaaaatacg aggtctacga ccctctaatt tagcaaattc atcagctaat tgctgagctt
      961 tgcgaaaatt ttcattttga ctaatttctg aactatacac acctgttatt gatttaattt
     1021 ctggagtata acgtccccaa gctttttcca ttgcatagct aatttctcca agtgtagctc
     1081 tatgtttagc tgcttcgata gctaactcaa gcaaattacc ttcaccagtt tcgcaagctt
     1141 ttgtgatagc atctaaagct tgttgcactt ttttattatc tctactagct ttaacttcat
     1201 taatgcgttg tatttgcttt tctctaacaa tagtattatc aacttctaaa atttgtatag
     1261 gttcttcttt ttctaatttg tatttattaa cacctacaat agtatccaat ccagaatcta
     1321 tacgagcttg acgacgagca gaagcctctt cgattctcat ttttgggata ccagctatta
     1381 tagcttttgt cataccacct aattcttcta cttcttgaat aagtgcccaa gctttatgag
     1441 caaggagatc tgttaaagtt tctaaataat atgcacctcc ccaaggatct attactcggc
     1501 aaatttgggt ttcttcttgt aataaaattt gagtattacg tgcaatacga gcggagaaat
     1561 ctgttggcaa agcaatagct tcatctaatg cattggtatg caaactttga gtatggccga
     1621 aagctgctgc catggcttct atgcaagtac gagccacatt attaaaaggg tcctgtgcag
     1681 taagtgacca tcctgatgtt tgagtatggg tacgcagaga catagattta ggattttgcg
     1741 gattgaattg tttgacaatt ttagcccaaa gtaatctagc agcgcgcatt tttgcaattt
     1801 ccataaaatg attcattcca gcagcccaaa aaaaagataa tctaggagca aatttatcaa
     1861 ttggaatacc tgcttttaaa ccagtacgaa gatattctaa tccatcagct agtgtatatg
     1921 ccaattcttg gtcagctgtg gcaccagctt cttgcatatg ataaccacta atagaaatgc
     1981 tattaaactt tggcatatat tgagaagtaa atttaaaaat atcagcaatt atacgcatag
     2041 actgctctgg tggataaatg taagtattgc gaaccataaa ttctttcaaa atgtcatttt
     2101 gtatggttcc ttttaattct tcaagtttag caccttgttc aagagctgta acaacataaa
     2161 aggccatgat aggaatcaca gcaccattca tagtcatact aacagacatt tcagcgagtg
     2221 gtatttggtc aaagagaatt ttcatatctt ctacagtatc tatagctaca ccagccttgc
     2281 ctacgtcgcc taatacacgt tcattatcac tatcgtaacc acgatgtgtg ggtagatcaa
     2341 aagctacact taaacctttt tgtccggcag ctaaatttct tctataaaaa gcattactct
     2401 cttcggctgt actaaaacca gcatattgcc tgatagtcca gggtttttga gtatacatag
     2461 tagcataggg gcctctcaaa aaaggtggaa tccctgctgc ataatctaaa tgttctaatt
     2521 ctacaatatc ttcataagta taaaaattct ttataggtat accttctgta ctgtcatata
     2581 ctacgtttgc aattattttt aattgaggat tattatcttc aaatattttt atttttttga
     2641 aatcaggttt cataatttat attttattca aattttacat tatttttaat tttttatgaa
     2701 aactctcaag agtctctaaa atattagatt tgatatgaat aaattcattc actccttttt
     2761 ctttcaaaat ttctatatat tcagtaggat tgccagctat tactacgtta acattaggga
     2821 aagcattatt aatagtattt agaatgttta aacctgtagt gccgtattct tgatcactac
     2881 tacataggac tataatatcg gggttatgtt gttttattaa ttttaacatt tcttcatcat
     2941 tatcatatgg ttcagcatcc atgatatcat aacctgcaac tccgaagaaa tttcgcgaaa
     3001 aattagctct tgcattacgc atagctatat caccaaaagg caataaataa accaaaggtt
     3061 ttttatttcc ttttaataca tgttgttcag tgcaaaaacg taaattttca aaaggttctg
     3121 ctatacgaaa tattcttaat gctttttcat ttttagattc acttattaat tgttttttaa
     3181 gttgagaaga tatttgttca ttaaaatttg ggtaattatt aactccaaca atgtttaatt
     3241 ttcttttcaa aacagcatct tgcttttgtt tagcactttt gtctacttca tttttgatcc
     3301 aacccttatc cattagtttt ctaaatccac ctaagtcatt aatttcatta aaaagattcc
     3361 aagaatgttg cgaaatagag gcggataaat tttcaaacag gtaagaacca cctgcaggat
     3421 caataacttt atcaaaatga gcttcttcac gaagtattat ttgcacattt ctagctattc
     3481 tacggctaaa atcattagaa tgattaagag taatatcgtg aggcaaaatt gtaatttcat
     3541 tagcgccacc tattacagca gccatggctt caatagtatt tcttagcata ttattgtacg
     3601 catcataagc agttttatta aaaatgcctg ttttgctatg aatagtaatt ttagcatttt
     3661 cattaggtat gccataagcc tctaaaatct tagctatcag cattctagta gcacgtaact
     3721 ttgcaatttc taaaaaatag gtacttccaa ttccgaaagt aattctaata cgagggatga
     3781 ttttttcgat ttctatatca ttatcagtca aaatatcaaa atattcaact agttgagcta
     3841 atgaaaaagc aatttcctgt atgatattag cacctgcatt atgatatatt attccattaa
     3901 tattgataat tttaaattcg gggttccaat ccgaaacaaa ttgaaacaaa tttaataaac
     3961 catttatatc atctttatag ctattatagt attcaccatt aagtaaataa aaaccaaaag
     4021 gatcaaaatt aaaagaacct tttaattgat taatgtttaa atgatgttca tttgcccata
     4081 ctttaattag ttgagccaaa gaataatatg agtgtaaatc attaaaatgg agtgaatatt
     4141 tagctatgtc aatatcttga agcaaaatat ttaaataatc tattttatct atattttgaa
     4201 tatcaaactc tactgcatct gcgccatttg ataaggcttt caaagctgct tcattagcgc
     4261 attttaaatc ttttgcatca atattttgaa ttacttgcca ttttagtttt ttatcattat
     4321 aacctcgtaa ataggggtaa acgttaggat aatatgtaag ttcaagtaaa tcttcgatat
     4381 cattttttgt ataaaagggt tttacttcaa aaccctcatc agttttccaa ataattttta
     4441 aaatatcagc cccttttaga ctatctttta tcgcattttc ccactcttca ggggaaactg
     4501 ggggaaattc tgcaaaaagc ttttcttttg gcatattata tatcttttct ttttgcaaat
     4561 ttaagcaaaa tttttaaaat attaaaagaa atagaatgtt aagttaggga gttagatttt
     4621 ggtaactaat atatgtttaa attaacatat gttatattca ctaactccct atatcgatac
     4681 cacgcccacc tgtccgaaga tgcgaggttg agcattcgcc cagtattgct tacagtcttg
     4741 cctgtgaggt atttacccat cactacatac cacataatgt agttttgcag gtatttggta
     4801 gctacgcccc taaatttgaa atatatccaa ttagtgaatt cactagcctt tttcttgacg
     4861 gtacttaggc tatatatacc tcgcttttgc ctattgctag ttactatttc tttgtgcttg
     4921 attacattcc ttctaacctt tttgaatgct tttttattac tgctacacat gactccatcc
     4981 tttggcatct tagccttcag aaattgctct atttgttctg aacttagctt tttgccatca
     5041 aattttttga ataataaatt gccagctctg tctgtagcag ccaatacggc tactttcttt
     5101 ggcttttgct tcctagctcg cttcagctct tcggcatttt tgtatattct gcctttttcg
     5161 gaataatcca tcaatagttc atccatctct actacaccga aaaaattgat attatcttcg
     5221 aaactttgta gagctgctaa tatcttgtgt cgccaagcaa agcttgttgg taggctgatg
     5281 cccacctctt tagcacaagc tctgagactt ttgcctgcta ccatacatct gatgtaatca
     5341 agcatttttt ctttcttttg caacctgtaa acaaaagtac cagtggtaac tctaaattgt
     5401 ctgccgcagt ctttgcagcg atagttttgc atcccatttt gtaaaccatt tttaataatg
     5461 tgattgctac gacagtctgg gcatagtggt ggggcactga gtattttcag accttgataa
     5521 tcgactacat cggcttctaa ttttttcatt aatgaataca ggttgtctaa gaattcttta
     5581 ttctcttcct tactcatagc aagatattta ctggtgtttt ctacaatttt tttcatgaat
     5641 taaaattttt ataaaaatat aaaaattatt taacatataa acttgaaaaa cttaaacatt
     5701 atgtaaattt tatagaaaat tataatttca catccatcct tattgtttga caacaaagat
     5761 aatgctataa cattaataat taattatcta cctaaaaaaa ttaaatcttt atttgatagg
     5821 ataaactttg ggataattat atttaaattc atacttttct atatgaccta agatatgata
     5881 aagaaaatca aagtatgatt cagccatttg aaattctaaa ggattgtaat aatcaagagg
     5941 tagtggtaga ttatctacat cccccatttc tatagctgta gtattatatt cttcaacgta
     6001 tggatagctg acgatttttt tcataacagg cattttgcca aaatcttccc ttaataaaag
     6061 atctaacatt ttatctgcta gagctactgt tagttttcga tcatattcat aacatttacc
     6121 acttctcgca taataaccaa ttatttgagc tcgtgcatct atttttaatt ttttacttat
     6181 ttcgctagct atcactccac taataccgcc taagcgagga tgtccatatt catccgtttc
     6241 agtgcttgca tatactactt ccacctgatc tttttgatca ttgtaccagc gagttccttc
     6301 agaaacagca attatcaaat aattatcctt ttgatatttc ttttttatac attcgaaaaa
     6361 ctcttctttt cgttctttag tgatttcata ttcagggatc agaaccatat cagcaaagcc
     6421 gtaagatgct gcagcagtga gccaacctgc atttcgtccc atcacttcta ataccatcac
     6481 tctattatgt gatgcagcag tggttcttat tttttgagta aattctacta cagaatttgc
     6541 agctgatgga aatccagggc ataagcatac ttctatttct ttacctttat aattatgaat
     6601 tgtttgtgta tgcaaatcat tatctatagt tttaggaaaa cctataactg gtattttttc
     6661 ttgttcccac aattttttag ctgcaccatt agtatcatct cctcctatgg taactaaaat
     6721 atcaatacta tagcgatcca aatttttcaa aacttgatct acacgacttt caggattttt
     6781 tttcgaagga aaaggattag tacgactaga ctttagagca gaaccaccac atttaccatc
     6841 aaaaggttga tttgttaaat ctactatatc tccatcgcct aataatccct tccatccttt
     6901 tctaatgcca taaatctttg cacctaagat agctacatga ttgcgtattt gctctatcga
     6961 cgaattaacc gctggggtat ctccaccgcc agtcagtatc cctacagttt ttccttctaa
     7021 caattttttc tctctcataa ttctttattt tttaaatatg aaattatatt attttttgaa
     7081 attttcactt gctctccact atacatatct ttaatagata cttcatcatt tgctatttca
     7141 tcttcgccta ttattataac atatttgaat tttaattcat ttgcataatt aaattgtttt
     7201 tttagtttta cttcgtcagg atatatatct gctatgaaac cagctttatg caaatctaat
     7261 aatattggta aggaatattt aagataattt tctccaaagt ttacaattaa tattttatct
     7321 tctctagtaa taaaatcagg aaaacgattt aatttttcta atatatcata aatgcgttca
     7381 gcaccaaaag atatacccac accagataat ccctgtaatc cgaaaagggc tgttaaatcg
     7441 tcatatctac ctccaccaca tatacttcca atagacattt cattactgat aatttcaaaa
     7501 atagttccag tataataatt taagcctctc gccaatgtaa tatcaaaaat aagattatta
     7561 tttaaattga taaaatctaa atttcgcatt ataaatgata tctcatctaa acccgccagc
     7621 ccaacttcgc tatcgccaag gattttattc aaatatattt ccacttcact ccaatctttg
     7681 ttcgtagatg aaaaattaat taaatttttt aattttgtta tattgttatc tgaccaacct
     7741 gcttctttaa aagacgataa tacatcctct aaacctattt tgtcaagttt gtctaaggta
     7801 attgtgaatg gaataaatcc ttctgcatct acacataacc attgagctat accttgtaaa
     7861 atttttctat tatttatttt ggtagtggta gacaagttga gttgtgtaaa cacctcatct
     7921 gctagagtta ctaattctat ctcagctaat aatgaatctg taccaatgat atctatatca
     7981 cattgataaa attcgcggta acgtcctttt tgggggcggt ctgctcgcca taccggttga
     8041 atttgatatc ttttaaatgg gaaatttatt tcatttctat gcattactac atatctagct
     8101 aagggtacag tgagatcgta tctcaatcct ttatcgctaa gatattctat ccatgagttt
     8161 atatctttgg ttttccataa atgatcgggc agattattta aaaaatcacc agaattcaaa
     8221 attttatata tcagtctatc tccttcgtct ccatatttac caagtagagt gctcaaattt
     8281 tccattgcag gtgtttcgat aggtaaaaaa ttatgcaatt caaatatttt ttttaaaata
     8341 tcaataatat aattgcgttt tttcacttga tccggtccaa aatcacgagt gccacttgga
     8401 attgatggtt tgattataga cattttttta tgcaaagtta aaaaaataat atacaattaa
     8461 taaataaaaa aatttagata gctacagcta aggttattaa aaagtttaaa ttataaccta
     8521 aaaattataa gcaatttaaa tatataatgt atcttaatct aataatatat ttagataatt
     8581 attatatcgt tgagtactta tatctccatt ttttactgca gctattacag cacaatcagg
     8641 ctcattgata tgggtgcaat tattgtactt gcaatttttt atgtaaggta taaattctct
     8701 aaaccagtaa ccgagttccc atttttggaa atttggtaat cctaattctt taattccagg
     8761 tgtatcaata atagctcccc cagatctcaa aaaatgcatt tcagcaaaag tagttgtatg
     8821 cttgcctttg agatatttag ctgataattc ctgagttttc aaatttaatg atctatccat
     8881 tgtatttaat aatgaagatt taccgacgcc actttgacct actagcaaaa aagttttatt
     8941 ttttataaaa tttttaagtt cttctatacc ttgtttagta attattgatg tcaaaaaaat
     9001 cttgtagccg atttgatgat aaatattata taataattga aaatattcat ctttttcatc
     9061 atataaatcg catttatgaa ataaaatggt agtctctaca ttataagctt cggctaaaac
     9121 taaaaatcta tcaataaatc ccattggagt aaaaggatgt tgaggggtta ccattagtag
     9181 agcaccgtct agattagtag caatgatttg atatgtggca ccagcttttg tagcctttct
     9241 caatatagca ttttctcttt cataaataaa agttattata cctgatggtt cagttttaga
     9301 ttgttctaat tctatttcta cataatctcc aacagctacc ggatttgtag ttttataatt
     9361 ttgcaatcta aacttacctc gcaccctaca gggtattatt tcgccagttt ctatagattt
     9421 tacataatac cagcttccag tacttttagt tactaatcct ttcatatatc aaattttatc
     9481 aggtgtgcca atacaagcta cactgatttt aatattttct atatctttta aaagatctat
     9541 taatgataca gtggtagctc cagtagttat cacatcatct actattaaga cgtgcttatt
     9601 agccaatgac ttgctatttt gtatagcaaa aactccattt agattatcat atctttccca
     9661 tttagttttt ttagtttgcg tttcagtgaa tttagtcctt tttataatat ttatagcaat
     9721 aggtattttt aatactttgc tgaccccttt tgctatcatt tctgcttgat tatatcctct
     9781 tactttttct tttttaggat gtagaggtac aggaattatt atatctacag ggggatcaat
     9841 atcatttaat tcatacccaa tcaatttccc aaaaaaagta ccaacatctt tatgtccttt
     9901 gtatttaaga gcatgcaagc aatgttgcaa aataccatct ttataaaaat agcaaagggc
     9961 ataagcatac tctatatttg cttgaccaat aaatttttcg tacagagggt tttcaacaaa
    10021 atttttataa taagtaaaac ctaatttaga aaaacaatca ctacataaga tattattttt
    10081 gtcataaatt ggattttcac aggatacaca agtttttggg aaaagaatat taaaaaaaga
    10141 agtaacaaat ttatccatta ttagctttta taataaagct tataagttat tctaatttca
    10201 ggctaccctc accgagaata taatcattag taaaaacaga aaaataataa atacctggaa
    10261 tcaaattgag attatctcta gcgtagtcta cgcaaacttt ttgagctttg ccagtataat
    10321 tgattatagt tttttctgaa aattgcaaat ttcttccttg ataatcaaat gaatagcctc
    10381 cctgtgtcaa tattaaacca tctggtctgg caatacgtat gtataatgta tactcaccag
    10441 ttggtactaa tttgttttca ccaatagtaa aacaaatatc aaacttgtct gtgcgtttag
    10501 ctttatcagt aggtgtagct ttattacctc taattttata ggcaatcggt gttatattgt
    10561 aagccaccat gtaagtagct tctgatatag tttttgataa agaatctgta atagtagata
    10621 attctattgc tttggttttt tcataagtta aatcttcttt tagttttaca ttttcaactg
    10681 ttagttcttg atttattcta tacaagctat ctatttgtct tacataactt tgggtgatat
    10741 ttcttagcct atctaattgc ctttttattc ttgtataatc tgcttgagag gctattagtt
    10801 tttctatttc tttagcttgc tcttttatta aactatcctg atctgatagt ttaatattta
    10861 atataccata ctcttctttg atcttatcat attctctcat tatactgtcc aattcatatt
    10921 tgagctgata agtttcttgt agagttcttt ctttttctac tactgcagtc tgaaatttat
    10981 tttgagaact gaaataaaga taaatgaata tagcattagt tactaaaagt aaaataatta
    11041 atataatgta ccaagtattt ttcttttttt cttttggtgc attatctatt tcaaatttat
    11101 cattccccat gttacttatg tcattcataa attgaatttt ttacaaaaat aattaaaatt
    11161 attgaaataa acttttaatt aataatttta aaaaaaaaac aatcacaaaa taattattta
    11221 tttaaaaaat aattattaat ttagcaaaaa acatttttat gaaaaaaaca attttattat
    11281 taacaatagg attagtattt ttttcttcct gcaaaaaaaa tgtagctcca atagtaatta
    11341 agaaaaatat tacttataaa gtaaactcaa ctgcagaaag gctttcagtt aattattata
    11401 atgaacatgg ggtcatggta gcatctgaga ttgtaggcaa aacctggcaa tatgatttca
    11461 aagctacccc aaacacttat ttatatcttg gagccaaaaa tttaactcct acaggatctg
    11521 taaacataga aatactagta aatgataaag taaaatttaa aaattctaca gttttgccat
    11581 atggtgctgt caattgtagt ggctttgtag attaatacgt tattttttta ataatatatt
    11641 ttttaatgat atactggccc gagtacctgt agatgataaa ggtgtaatat caaacgtgcc
    11701 tcccaattga taagttatta gtgtatttac taatgataac cccaatgttg ttgcaaaatt
    11761 ttggatatct aaagctgata tgcctatacc attatctatg atttctaaat ttataccgtt
    11821 atttactttt tctattttta cagttattgt tgggttttcc caatcgatag gaaatgcgtg
    11881 aattatgctg ttataaatca attctatata agaaagcata aatggaatag ccacatctac
    11941 atgcattatt atctcatcgg gtaaatctaa atacaattgt atttttttat taaaatcttt
    12001 tattaatcta ttagtttctt ttactatcac agcttttata tctgctacat tgctattttt
    12061 ggcaaaatat aattcatttt gtaaatatga caaaagtgag accttttcat atatatctct
    12121 taatgcagtg atggcattta aattgtttat attaatattt ttttgtaaat ttaattttat
    12181 taatgacaaa attaattgta aattattttt ggtacgatga tgtatctctt ttattaattt
    12241 atctttttca ttaatatttt ttagtagatt ttcttcgtac atttttctct cttctatgtt
    12301 atgcaaaagt accacagcta cattagaagt gctacgatag ctatatctac atgaaaatac
    12361 agcctcatat aattgtggtt ttcctgattt agaaatcaaa gacaattcag tttttttatt
    12421 tttgagtttt tttatgtttt tgaaaatatt aattaataaa tcataagtat cttttggtgt
    12481 aaatttagaa atattattat tcaataactc gctttcttga taatttagat atttgtagaa
    12541 tttaggattt gcatataaga gtgtacctgt ttgatcatat atgaataatg gttcttctat
    12601 attttcaata acttcattaa agtttagatt tatttgttct aattcattat tgatctgtag
    12661 cttttcagtg atatctgtga taaagattag caacctctta tcatctgaat taggataatt
    12721 aattaatgaa gcttgaatat cgaaatcata tctttggtta tctcttgtga tagttgctat
    12781 tttagaaaat tttcctgtat tatttaaagt actaagcaat tcatcgaaat ttataatttg
    12841 tttaaaagta ttgcactgtg ttagcaggca attattttcg tcgattaata tatattgttt
    12901 ggctaattca ttgcatagtt tgacctctcc atcagtagta gcatagatca cacctatcaa
    12961 atcattattt attaaatttt ctaattctat gtttttcttt tctaattctt tattagattt
    13021 tatcatttca gtgatgtcag ttaatgaacc tataattttt tctttaccat tttcaattac
    13081 catttttgag tttgtaaggc aataaactag ttgattatct ttatttatga gttttaataa
    13141 ttggtttttt aattgttttt tatctcttaa tttatttata taatcttctc tttctttagc
    13201 tgaataaaaa aactctacag ctggtttacc tttaagttct tctcttttgt aacctaaaaa
    13261 atcataaact gatggcgata tttctattat tacattatct aatatttcat aataaacact
    13321 ttgaatattt tctataatat tcttataatt atttatatta tcagaatatt tttttattgc
    13381 attttgtaaa tctaatccaa taatgtgata ggtaaagggc tctgtaatat catttgtaga
    13441 aaacgtatat gtgtttatga acgaaatcac cgtgctataa tttttttgta aaatatgatc
    13501 tggtatatta gatatatgta tttcttttat acaaatgttt aatttttttt ctatatgcaa
    13561 atcattcctt tcaaaggata aaaacttatt ataaaaagag aggtattgag atatgtcaaa
    13621 aaattgtaat gagaatggat ctgatagtaa aagttgattg tctttatttg ttattaatat
    13681 tgataattga caggtattga gagacttact actactatcc tttttataga tagtattttt
    13741 gatatgattt actatatata aatatttgtt taaaaataaa tatttttcat tactaatagg
    13801 tttttcaaat cctattagca tttgattagc acaattcaaa tatgcaaaat accaatattt
    13861 atttttatta atatcggatg agtcatggca atataaattt ttaatgttaa tttctacgaa
    13921 atagtcttca ttattttgtt ttataatttc aaaactatta tctaaatcat ttattttaat
    13981 attaacatta ttaataggta tattttttga ataaaaaaaa tcatttttct catgaccaca
    14041 gataagggta taattttcat taataaaaat attttgaaaa aaaagattta taatcattgg
    14101 tgataactta tcaaaaacga aataatttag ataagtaact accaatgata gcgaatttag
    14161 agataaagta gatttataaa aatctaatga atgatagtcg ttttccatat atatttattt
    14221 ttataatttt gcaaaacaat tttttcgtaa atgtctttaa tattaaaaaa tgccaatctc
    14281 tttaaaaata aatttaatga aatttgggta ggaaacgtat taattgacaa aaattcaaac
    14341 aaaaaggtaa tatattcaaa agaaataagt gaactactcg acaaatctag tgaggctact
    14401 ataatagatt gcaatagact tctcgtaatg cctgcatata tcaatgcgca ttttcattct
    14461 cagaattttt ttagtcagtt aatattaaca gatgaaacta ttttaggttt gcaaaaacaa
    14521 gaaaaaatat atcataaatt agataagata tgggatagag atttactaga agctgcggct
    14581 ttgtatagtg ctatcaattt attaaaaaat ggtacaatcc acgtgataga tatgctcagt
    14641 ggaccagcta tagcctctat agataatata caaataatct atgaaacata taaaaaagta
    14701 ggtgtaaatg tcatgcctgt tatagtaata acagacaaat atggatttgg agtagctgag
    14761 aatctattag atacaactga agcttggtta aaatctcata aagggctaat gggattagat
    14821 catttgttta atgtaggaac gggtacttta acagaggtaa aaagattaat ggataaattt
    14881 aaatgtggta ttcatggaca tttagctgaa tacgcttatg atcaatctca ttcaatagac
    14941 aagtataaat tgtcagtagt agagagatta gatgctttaa atctaattaa taatcctcga
    15001 aatatgtggg ctggctgttt gcatataact aatgatgaag tagatttaat aagagaaaat
    15061 aaaaataatg taatagagtt atacgaaagt agtcaatggg aaaacaaagg atactttagc
    15121 tccgaaggat tgccattgat aatgtttgga actgatggga tgcatagtga tatgtggcaa
    15181 actatgaaaa atacttatta tttcaataca gaatatgata ggatttcaat aaatgatgca
    15241 ctgaagcgtt tataccagcc tcataaatat ttttcaaggt ttttcccctc tatttatgat
    15301 cccgcttcat ttgtaatata tgattgtcct ttgataaatt gtataaatga agaaatgaaa
    15361 ccaatgcaat tattgcagct tatagaccgt aaatatatta aatacgtcat ttctcaaggt
    15421 gagatcaccg tagaagataa taaattgaca aaaataaaag aactagatgc ttatatcttg
    15481 ataaatagac aatttaaaag gttgaaatct ttatataata ataggcaata ttgactactt
    15541 attaatttaa aatgaagaaa ttaactaaat agaagttaaa taattaatgt aataaattag
    15601 taaaataaat cataatatgt agtatcatta aataaaggag tgatggtgtc tgaacgaata
    15661 atttctatag cattgtgttt ccaattagct actaagatat aaggtgcata agcattgaca
    15721 ttacatacta aaaagaggtc tacatctaaa ccattgacaa taaatgggaa atttatttca
    15781 gaattgaaat atgcatcatt aggtattatc caactaatag atatgaaatc gttttcatca
    15841 taaggattaa tattttttat atgaattttt attactcctt tagcagctat tgtgtagatt
    15901 ttatcattta tcaatttgtt acctaaattg atggttttat aaaaataacc gtctttattc
    15961 aatatcaatc tatattcatc ataaacatta tctccataat ttaattgaaa tctacctaga
    16021 tcatcggtat aaccagttga gattgtttga taagctaccg aaaatatatt atcttgcaat
    16081 acttgtgctt gtagatctaa tttaacatta gcaactggtt tatctaaaaa ttgatctttt
    16141 accataccag tgaaactatg ttctttaggc tctttatcac aaccagccaa taggattaaa
    16201 gcaattaata aaacaaaaat gttttttttc attatcaaat tttcataaac aaatttaaca
    16261 aaaaatattg atttacatta ctttccttta atttttacaa atgttttaaa tttatctccg
    16321 cgatcttcga agtttttaaa ttgatcataa ctgggagctg caggtgataa aagacaaatt
    16381 ttgtcaggta gagtatattt atatgcagca tcgatagctt gctccaaact atcgaatatc
    16441 attatatttt tatcttgatt ttcttttaaa ttattatata aaaatatttt taattctcca
    16501 atacaaaaga tattcctcac tttagatttt tttaaataat ctatcaattc agtgtaatct
    16561 atacctctat ccataccacc taatatcaat gtatctacat tgccaagaga ttctaatgct
    16621 gctatagtag attgtggaat agtagaaatg ctatcattat aaaaatcaat attttgatat
    16681 ttgcctacat attctaatct atgtggtaag ggttgaaaat cgtataagta atcaattaat
    16741 ttatcaatat caaaattgct taataggtat gatgctaata atgctgcact gatatttaat
    16801 aaattatgtt taccttttaa ttgcaattta tcctcatgta tgattaattt tttattaaaa
    16861 tttttatttt taaaaataaa attattttta gtttgataaa aaagacctcg attttcatta
    16921 atatcataat tgccaaattg ccaaatttca ctattgatca tttgaatttc tctcattgaa
    16981 ttattaatga ttgcgatgtc atgaatattt tgatgtgata aaatatttaa tttagcttct
    17041 ttatacaact caaaagattt ataataatct aaatgctctg gaaaaatatt taataatata
    17101 ctaatatgtg gcgaaagttt gatctgatct aattgatgac aagacaattc tatgacatac
    17161 caatctgcat ctgcatcatt aattaaaagt tcaaaaactg gtatacccat attaccgcca
    17221 attttagcgt caatatttat tgatctaagt aaatgatata ttaagctagt ggtagtagac
    17281 ttaccttttg taccggtaac gcctatggtt ttgtgtttat aatgtttcaa gaataattct
    17341 acttgtgagg ttagcttatc agattttttt atattaaaat tgtataaact aatgccagga
    17401 gatttaatta ttaaatcaaa aagatcaatt ttttctataa gttctgtttc tgaaatgtat
    17461 tttaaggatt tttcttttat tttattttta tcagctatgg ttatattggc attaggcaaa
    17521 aatttattta taaatgaata tgtgctttgt ccttctctgc cccatcccca gatacatata
    17581 tttttattaa taaattcttc aatgatagga ttcataatta tataaaatat ttagtaaaat
    17641 tatataattt tttaaaatgt ttggcgttaa attttaaaaa ataaatcata acaatatcaa
    17701 atctaatttt ttaattaaat gcttaactaa taatacaata gtggcattat tacgtattca
    17761 tccaattgtg tatttggata atgtttggtg attcattcct gatattcatt tgcgatttta
    17821 ccatgttcat ttaaaaccat tttattatta atgatttcgc caaacaaata ttgcatataa
    17881 tatgtgaata attataacag cccaaacgtt ttgaaaggtg atgataattt gtatttggtg
    17941 aagtcatgtt ttagtatatg taagtgcgta tgatgatcat acgcacttac gaggtgtttt
    18001 tcattggggc aatttgtgaa ttgccccaat aacgaattat ttcgtaattt cctattttat
    18061 atttagtttt ttcacaacaa attcttgtac ttttaaagca agttctaaac atttttttgc
    18121 ttcgtcaatg ctcggaatat aaaaaaattc tgggtatcgt aattcggttg catatacagt
    18181 tagctcatga acatctattt ttgcaagttc ttcgaattca gtatcaattt ctatgcataa
    18241 tctaaggatt tctgcaatat tatgagtttt gtgaaaagat ttgttattat aaacaagaaa
    18301 tgctttcaaa tatttttcta cacattgctg tgcatgaaag caaataacat cagttattgg
    18361 gtcttttcct tctattaaat attttactac tttcaaatca ttttctgctt ttttaatcca
    18421 atttttggtc tcttctatat tcatatttct atcccttcct tgtttgcata ataagatatg
    18481 ttgccaacaa ctttcataga tttattaaat tgattgatac ctctaaatat taaatcattt
    18541 gagaaattga tagctgcaag ttttaattga atcctaccag taagtttttt taattcgtta
    18601 aatgtaatat ctttgtctaa aatgacaagt aaatcccaat cgctttcttc ggtataatcg
    18661 ccccggactc tactgccaaa aagtatgatt ttaacaacat taaaacctgc cttttcacat
    18721 tcttcagtga ttattttttt gacttctgta agatctaaac tatcatcttc catatctttt
    18781 taattttttt tctagattaa ttcttttttc aaaaaccttt caaaattcat atttttttga
    18841 ttttaagggt ttatgtgcaa atttaataat attttttaat aaacaatttt tttttaccaa
    18901 tttataataa tttttctata ttattataaa aaattcctta ttaccatata aaaaaccatt
    18961 atgaatgata aaataattga tataaaattc attattgtct aaacctctga ttttattata
    19021 atttttgtga ttgggcgtat ggcaatacgc tcctatgatg acttattcaa gagagcaatt
    19081 cattagggca attcatgaat tgccccaata atgaatataa taccatggat gtgatttggc
    19141 attattacgt attcatccaa ttgtgtattt ggataatgtt tagtgattca ttcctgatat
    19201 tcatttgcga ttttaccatg ttcatttaaa accattttat tattaatgat ttcgccaaac
    19261 aaatattgca tataatatgt gaataattat aacagcccaa acgttttgaa aggtgatgat
    19321 aatttgtatt tggtgtattc ataattttat atgagattta taaacaaaat attagaattt
    19381 ttattgatat tttttgttat atggcgatat gatatagagt gcatgaccat ataccgcaat
    19441 aaattttatt gatttgaata ataatcgccc aaaaatatga ccttacttat aggctaattc
    19501 atcttcgata ggagctaagg cttctaatgc taatttaaca aaatcattga ggggtatacc
    19561 tattaattcg cattccatta ttgtatctct gtttacgtta gcagcgaaac gtttttcttt
    19621 cattcttttc actactgatt gtactttgac atcactgatt tttttagaag gatatatgag
    19681 agctaccgca aagataaaac cagataatgt ttctgcagct gctaatgcgt ggtgaaagtg
    19741 tttagaacgt aagctttgtg ctgctatctc attatgcaat actatggctt ctataatatc
    19801 attatcgaat ttttcttttt gcaaaatttc ttttgctttt aatccatgaa gtgtagggac
    19861 accatttgtt aattctacat ctatatcatg tagcaaacca gctaacgccc acttatcctc
    19921 atcttcacct agataatttg ccaatttttt cataatagct tctgatgcaa gtgaatgagc
    19981 tattaattta ggattgctaa cgtgtttatt taataaatct aatgcatatt ctctattaat
    20041 cataaataaa ttttttagaa aatattaact aaggttgtga aaattttata acaaatatga
    20101 gaaattcctc gtttatccaa tccaagactt tcgactttga ctcctaaatt tagtaatata
    20161 gatataaagc taccaaagat aaacattact aaaatgacac aaaaaatcat acctaatact
    20221 ctattggcag ttcctaatac tggtatttta ttaaaaatag catttatgat tttagctgca
    20281 aattttacta ataatgctcc gcctattatt agcacaatca tagtaaggat ggtttgaaat
    20341 attgaagaat tttgtggaat cagctgcatg gcaaaggcat ttttgcctat tataccaaat
    20401 aaaagtaaaa caagtaggta gtatccaaca aaaataacga gctgacgtag aaatcctgtc
    20461 attatacctc taatagcagc taaaactaat aaaataacaa caataacttc tattgtactc
    20521 ataatgattc taataatttt tgagcgagtg atgatgcaaa tataaaatct tttaattctt
    20581 ggttatcagt ttcaaatata ttatccttag aaccttccca ccaaagcata ccattgtaaa
    20641 agaaaattac tttttcacca attttaatga cagagttcat gtcatgagtg tttactactg
    20701 tagtagtatt aaattcgtgt gttagatctg atattagctc atcgataaga accgcatttt
    20761 gggggtctaa accagagtta ggctcatcgc agaataaata ttttggattt agagctatag
    20821 cacgagcaat agctaaacgt tttttcatac cgccacttag ctctggaggc tttagtttat
    20881 tagtaccttg tagatttact ctttctaaac aaaaatttac cctttctaac ttttctttat
    20941 tagacatatt agtaaacata tccaaaggga atttcacatt accttctaca tctaaaaaat
    21001 caaaaatggc tccactttga aaaacgactc ccatttcttt tcttattaat ttttgttctt
    21061 tttctgataa tgaataaaaa tttttgtcac cataatataa ttcgcctttt tcaggtatca
    21121 aaagtcctaa caagcatttc ataaaaacag ttttacccga gccactcatc cctatcacta
    21181 aatttacctt acctggctca aatcttgtag taatgtcttt taaaaccact ttatcatcaa
    21241 atttttttgt aagatgctct actctaatca taataaatta acctaaaaat atttgagtca
    21301 aaataacatc aaaaataata ataaaaatac ttgattgtac catagccttt gtactatttt
    21361 tacctacctc tagtgctcca ccctctacaa tatatccata aaacccgctg atagaagtaa
    21421 ttataaaacc aaaaaataag cttttagata atgcataagt aatctcaaaa ggtgtaaaaa
    21481 aagctctaag tccataaaaa tactcgcttg gaggtatttg tgtagagcca atagtaacta
    21541 aatagccacc aaataaccca ctaaatatac taaaagtaat aatcacaggc ataataatca
    21601 caaaagcaat tattttaggt aagatcaaat atgatgccga attgattccc atcacttcta
    21661 aagcatctat ttgttctgtt accttcatag tacctatctc agcagctata cgtgagccca
    21721 ctctacctgc taagagcatg ctcataatag taggcgaaaa ttcgtatatt atcactactc
    21781 ttgtgataaa tccagctgta tatttcggaa ttagtggact ctctatctga aatgcagttt
    21841 gtatgataac aactatacct atgaaaagac ttagaaaaca cacaataggt aaggactcta
    21901 cacctaaata atacatctcg tgtacaactt gatttgcaag ggttttaaac ttcactggtc
    21961 ttgtaaaagt ttttttcata acgagcaaat aatctgcgat cccttcgaat aaattcatat
    22021 catttataac ttttttatta aaaaaattaa ttactcaacc acaataatgg attctctggt
    22081 tctttgctat cagtccatat ctcaaaatgc aaaatattag ctttataatc tttcaaaaaa
    22141 ccagtttttc caataatggt tcccttattt actttttgat tttgttttac atttacagta
    22201 gataaattag aataaacagt aagaaaatct tcatgtttca ctattacagc ttttaattga
    22261 ttaggtagtg aaaaaatagc tgatactgtg ccatcatata cacatttgac ccactcatct
    22321 tcttttacat tgtaatctat gccattgttt tgaatagtta tattttggta aactggatgt
    22381 gattggttgc caaaagtgcc tactattact ccattatcta cgggttttga taatttgcct
    22441 ctataaatgc tagcaataga tgggctcgct ttagtcgccg gtgcacctga tggtgactta
    22501 gatgcttctt tagctttagc agctctagct ctttctaatt cttttattat tatttgtatt
    22561 tgtttttcga gttcttgttg tactttcttt tgttctttta tttgtttttc taaattggtt
    22621 tcttttttct ttaattcatt tactagtata tttttatttt taatttcttt ttctataact
    22681 attagtatag aatcctgctg agctaataat ttttgttttt cctcttttaa ttgttcatat
    22741 tcttgctgta gatccattaa tttttgcaaa gaagcttgat aatttttgat ttgtaattta
    22801 taaatattag ctaattgttt taggtattga taacgttcaa aagcttcatt aaatgaatta
    22861 gcactcaata caaaagaaat tctattttgt tgtgttttgt acacatacat tagtgttatt
    22921 attttactaa gtcctacaga aagatcttga atattttgtt gcaatatggg tatctcttta
    22981 gaaatttcac ttaatttagt attagcatac gccagctcag atgttatgat gtttctcatt
    23041 ttttgattat ctcgtagttt agcgttcaaa agatcgagct ctgctagaga attttttcta
    23101 tttttacgtg tagattgcag tagtttttca gtgtttttaa tttgtttttc taaatttgcc
    23161 ctttcttgtt ctaatttttt cttttgagct tctgtttggc ttaataatgg tatgcaaatc
    23221 aataatatta ttataaaaat aaattttgag tattttatgt gaccaaatct attcatttac
    23281 aataatatta ataaaaataa tatgcaaaaa tacatttaaa aattcaaata agatattaaa
    23341 aagtattata gaatgtaaaa taaataaaca gaaattaatt tttgataaat tttttaatac
    23401 ttaatttttg taaaaaatat tgataaaatt tctgtacatt atattactca ttcatttgca
    23461 aagttttata taatcacaaa aaaaattatt ttacttggat tttgaatttg tataatgcca
    23521 aaatcaatga tatcgtgtaa tttattataa actatatggc ctttgtataa actcgatagg
    23581 cctaggaatc gaaatttcct tcatgcagtc gatagcaaat ttatctgtca taccagatat
    23641 atagtctgta acaatttgtt tgcatagatc tccctcggtg agattatttt gttgtttttc
    23701 attaatataa aagtttttca ttcctttaag gtaattacca aaaaatatat caatatttag
    23761 gttgttttct tttagatacc aatctatctc ccaatgatat tggcagaaaa gtttagtaag
    23821 ataatcaaat aaagtttcaa ttatcctttt acagtatatt ttatattctt ttagtcgggg
    23881 gtgatcatat atatatttat agttaaaatc ttttaatttt agcattaatt cataacattc
    23941 atcagaaaag gatatttcgt catcagtaga attatctata acatccaaaa ctaaatgatt
    24001 gatgatctca ccattagaag tacctatttt gtttttaata atatctggta tatcctcttt
    24061 tgaaaaaaaa ccagctatga tggcatcttc tatatctcta ccaagatatg cgattttatc
    24121 agaaaatctc acaatgcagc cttcgtaggt agtgggtaaa tgttttctat cagtgatggt
    24181 gtctaaattt tttattgttt gatctggttt gatgcttttt tcaaagcttt ctccattatg
    24241 aaagatgatg ccatctttta ctgcatatgt tagattgagg ccttcgcctt gatttgtaag
    24301 tttttcgaca actctataac tattaatttc gtgaatgaat aaatctttgc caccaagcaa
    24361 aaaagataac tcagattctc cagcatgccc gaatggtgta tggcctagat cgtgtcctag
    24421 acctatggaa taagctaaat cttcgtttaa tgaccattca gaattatctt tagaatttaa
    24481 gcctctgcat atagtagcag atatagtggc tacatggagc acatgctcga ttctcgtgca
    24541 tacgtgatca tcatcagggc tgaaaaaaac ttgtgtttta tgctttaaac gtctaaaagg
    24601 cctcgagtga ataatagcag tctgatctcg ataataacat cctctgatat cttcatcttt
    24661 tttataagat cttttttcta aagcctcttt aggtagtata tttttactca tttatttttt
    24721 gtttgtatat aataaatttt tatttactac aaatataata aaaaaaatta taagtagaga
    24781 gtactgttct ttattttatc attaaatgat aaaatatgat aaatattttt tagacgattg
    24841 taaatatgtt ttcatagggt atatcaatat tggtatttag agatattgat tatgatatta
    24901 tttgaaaaat atccgttaaa aatacaaatc acatggcttc ggctactaca aaagcactac
    24961 cgcctacata gattatgtca ttagctattg cttgtgattt agcggcattt aaagcattgg
    25021 aaacagaatt ataagtttta ccagacagat taaaattttt agctttttcg tttaaaagca
    25081 taaaatctaa tgctctaggg actttgggac tacaaaaata atatattgca tcttttggta
    25141 ataagcttaa aataccatct atatctttat ctgccatcat gccccataca atatgcaaat
    25201 gatctttggg tatagattct aaaattttaa ttacattact cactgcattt acattatgtc
    25261 ctatatccat aatgatagga attctatctg gtacataagt ccatcgtcca aaaaatccag
    25321 tattatcaaa aacatttttt ataccattta ttatatgttt agtagaaata atatatttat
    25381 acgtttgcaa aatattaatg gcagataata cagttttaat attatctatt tgaaatttac
    25441 ctatcaatgg agaataaata tcaaaaaaat catcattata attatttttt aaatgcaatc
    25501 gcatagaagg tggtaataga ttttgagaaa aatctattag tttccattca tcagctgaga
    25561 tgatgagtct agcattgttt ttataagcaa aatcaataaa aactggtaat gtttcttgat
    25621 cattttcacc tattatacat ggtatgccgt ttttgatgat accagctttt tctgcagcta
    25681 tctcttgtaa agtatttcct agtaaattag tatgatcata agaaatattt gtaatgatag
    25741 aaactaaagg tattatcaca ttagtagcat ctaacctacc acctaatcct gtctctatca
    25801 cagcaatatc tacgttagat tgggcaaaat aagaaaaagc catcacagta tttatttcaa
    25861 aaaaacttgg ttttagttgt tctaacaatt taaaattttt agtagtaaat ttagctatcc
    25921 attgtgcagg tatcatctca ccattaacct ttatacgttc tcgcattgtt tttaggtgag
    25981 gagaggtgta taatccgact ttgtatcctg cctcttgcaa tatagaagct atcaatgaac
    26041 taacacttcc tttgccatta gtcccggcta tatgaatcat tggaattttt aagtgaggtt
    26101 tatcaaattt atttagcata aataatgttt tttctatgcc accttgatag cctgatgaac
    26161 cttgccgctc atacattggc agctgctcca taataaattt ttctgcctcc aaaaatttca
    26221 ttaaaaaaaa attttttaca aaattattaa taaatcaaac aataataaat attaaaatcc
    26281 tttatcgcaa ttgtaacaaa tatctaaata tttgccttta atggattgaa ttcttatttt
    26341 atttaattct tcagaaaacc agatctcatt tattgatttt ttgtaccaat tatcatttaa
    26401 aaaattggca tttttatctt ggcagcatat tgcgatttgt ccattttgta ataatattaa
    26461 ttctgtccat aacctcgtac aaggagtaga gggtaatttt tttaatatta attgatcatt
    26521 tagtatctta tatctgctaa atcttttgtt tttgggtaaa aaatcaatag cattctccac
    26581 taattgggca cttttgatac tagtttttac ttttattttt ttcatatgtt ttttaaaaag
    26641 tctaatttca tgttcattat gtctaaaaac tacaaattgt acaataattt tgggagattt
    26701 tttctttaat atatgttttg cttttattaa attatttatg gcatcataaa ccttgtctat
    26761 attgccacca gttctatatt tttcataggt tttttgagat atgccatcca tagagacaac
    26821 tattctattt aatccgctat ttactaaaga tgtagccaca ttatatgaaa tattctgagc
    26881 attagtagat agctctacat aaaagtcatt agaacgtagg tattttattt tttctattat
    26941 agtatcatct aataaaggtt ccccttgaaa atataaggtt atccaaaaaa tatttttagg
    27001 taaattatta attaatcttt gaaaatctgt aaatgcaata aaactatcag ctctatttat
    27061 atttcctaaa ccttgttcgc aaaataaaca tgagagattg caaagactac aagtctctat
    27121 atgaatagca aatggtaaat ttaaaagaca aaatttacca aataatttag aataccagta
    27181 tgaaaaagca aataaaatta aattataaaa tctattaata ctttgtttga tttcgaaaaa
    27241 cattttagtg aaaatttaaa aatctaaatt acaaattaca aacaaaataa aacaattagt
    27301 tatttttatt ctaacacata acaaattgct tatttgtgat ttttcagtat taagtcataa
    27361 aaatgtaaat gggaggttgt atttattttt tttcaataga tttttccaga tcagaagtaa
    27421 tcttttgtat caatgggaca ccttgcaaag tttgaacagc tattattctg agcaaagtat
    27481 aaactggtac acttagtatc ataccaataa taccaaataa ttcttcgcct attactaaaa
    27541 ctaaaaatac ttcgaatggt gtacttttaa cacttttagc atatatattt ggttgcaaaa
    27601 caagattatc gagtattttg acagattcga atccaatgat agtaatcaca atattccata
    27661 gcaattgtgt gtcggctccc gacattagcg aagatacaat acctaataaa atagctaaga
    27721 tcaaagctat caaaggacca aagtatggaa taatattgaa taatccagca aaaaatgcta
    27781 ttaaaatagc ataatcatat cctatcaaag tgaatactaa aaaaattaaa ataaaaacaa
    27841 tgatctgatc tattagaatg ccaagaaagt atctagagag taaaactcga gtgtcattga
    27901 tgatattgtc tatcatgcta tgatagcgtt cagcgaaaag cgattttact aatgaaggaa
    27961 aaacattctg atctttaata ataaaaaaag agaaaaacac aactgtaact acgcccacta
    28021 caatatctcc gaatgagttt ataatgtttt caaatgcttt ttctatgttt atgctagctt
    28081 gaaagtttgt aatatacttt tgaattatct gagcaaagct aaaactttca ttagctattt
    28141 tataatcaat caatgttttt tctattggtt ttaaactgga ttctaattgc aaaagtaata
    28201 tatcatagtc tatagcaatt agagaagtaa gtttagtaat aataggtgga agtacataaa
    28261 agaaaaacaa aataataact cctaataaaa acaacataga tatgatagta gctacagcat
    28321 cattcaaatg aaatttttta atacgtattt ttaaaagtaa attttttatc ggggctaata
    28381 taagtgatat aacaaaggat atgagtaaat aaagtaatac cttaggtaat aaaataaata
    28441 tcaaagctat cagtatcaaa ctgggcaacc aaacatacca ttttttaaat atattacccc
    28501 aaaaatgttg gacattgttt ttgttattca tatcgagcta taaatatttg aatttaataa
    28561 attcatctaa tacaagtttg tgatcaaaag ctaatggtgg tagtagtgat aataggaacc
    28621 acttcgcctc agctgcatca tcacccgctt gagtacgact taataggaaa tcatctggta
    28681 aaatggcata aaaaacaata gtgatcactc gtgtacgggg atcccttttg ggatcactaa
    28741 aagctttaaa ttgttctaaa aaacatatat ctaatgaggt ttcttctttt agctctctac
    28801 gagctgtatc ttctaaagtt tcattctctt cgttaaatcc acctggcaaa gaccacatgc
    28861 ctttatatgg ctcattggct ctcttcacaa gtaaaacaga ttgtttgtgt atcaatatga
    28921 tgtctgtagc tactgctggt ctaggataat cgtagcaata ttttttcttt ttcaacataa
    28981 ttgttttaaa tttaattaca aatttaaaac aactatttag aaagaatatt taattttaaa
    29041 aaaaatttca aatattttga tatatgagaa aaatttattt atttatcatt ttatttatca
    29101 cagtatttaa tggtaaagct cagactcctt tgaccatagc agtagatttt actacttcag
    29161 atgttaatgg caatgttcat aatttattta actatttaga taacaataaa tatgtattgt
    29221 tagatttttt ctttactcgc tgtagtgcat gtcagacttc atctccgcag tgcaatgcat
    29281 cttatattta ttttggttgc aatcaaggtg atgttatatt tttaggtata gattattatg
    29341 atagtagaca ggatgtaata aatttcatca atcaatacgg acttgattat ccttgcatta
    29401 gcggagatga tggtggaaga caaatatgcg aaatgtatgg tatagatgct ttccctactt
    29461 acatacttat tgcacctaat aagaacattg tagaacgtga tatttggcct tttagtacta
    29521 atatatgcaa ctctatatta caaagctatg gattacaaac gatgagttgt caagaaaata
    29581 ttaatgattt ttatgatgaa aatatttcta tttatccaat accagccaag gacgagatac
    29641 tcctaggctt ttataattac tatttaaatg aaccaataat atttatttat gatcttactg
    29701 gtaaaatgat atacactgaa ataataacca acctatcaaa tactcacaaa atggatatat
    29761 ctgcaataga taacggtacg tatattttat taatcgtaga aaataatgag gttttgttga
    29821 gaaaattaat ttctgtgtct cattaataaa tttttattta gtaaataata tactgtatca
    29881 attatttttt ttaatagatt aacaaaacta atcctttcag atagtaacct tctggatgat
    29941 agagtgaagg cgcatgacat ggagcttgag taagtctatg caatatttta atatttctac
    30001 cactatctaa tgctgcattc atcactgaac cttcaaaatg taacgatgat acagcttggg
    30061 aacagctaaa tgtaaataaa ataccattat tttttatttt ttctattgct ttactattga
    30121 tacgtttata tcctttcaga gcattgtgtc tggtatttat agatttagca aaggctggcg
    30181 gatctaaaat aattacatca taataatttt cttgcatatt atttagataa tgcatagctt
    30241 catcctctat ggcttcatat ctagttttgt ttattttatt taattcaaaa tttttatata
    30301 ataaattaat agcttctcga ctactatcta cagagactac ttttgtggcg tcagctaagg
    30361 cagcatatac agaaaaagca cctgtataac taaacatatt taaaacattt ttgtccttaa
    30421 cgtatcgttg tagcaatttt ctattttctc tttgatctag ataaaagcca gtcttttgtc
    30481 cttcttccca atttacataa aatgaaatat tgttttcttt gattatcaca ggtatttcta
    30541 tgttatctgt ttgataagca taataagtat ttttgatatt ttgtgtttta tcaattacaa
    30601 aaatcgattt taatttttca cgataaattt gttttagagt ttcaataata taattaatat
    30661 cattatacat accagtagaa tgagtttcta atactaaatg ataatcatag aaatctatta
    30721 ttaatccagg caatccatcg gcttcgccgt atactaatct aaaagcatta gttatattag
    30781 atagaggcaa attaatagct tcacgtatag ctacagccct cataatttta tttttgtaaa
    30841 attcgaccga acgattttcc ttttgataac taacaatacg gccaatgatg ctccctttag
    30901 tagaatattg agccatagct aaaaaattat tatgattatc gtatacttct acccattcac
    30961 cttctttagg ctcaccttca atttttttaa ctgctccact aaatatccat ggatgatacc
    31021 ttttgataga ttctactttg ccagttctta ttatcagttt attcatattt ttttgcaaaa
    31081 ttaagttaag tatttttaaa acatcaaata atgattattt tttttctaaa gtactctttt
    31141 tccatttcta aacttcacaa taaaagcatc attgtatcct ttatctttca cataattcaa
    31201 tttttgacgt gcttgattat aatctgtgaa ttcaccataa atatacttgt atgtattatt
    31261 aaaaaaataa taagaaatag aatctgaagg gaaatcttta tatattttat gagtatttgt
    31321 aaatatttca tttgaagata aaaattggat ataatatatt agttcttcat tagtttcatc
    31381 attagcttta gatagttgat tttgtgttgc taaaatagat ttctctttta aagggaaatt
    31441 atctacatta acaatataat ctgcaatagc attagctaaa ttctgagcta attttttttg
    31501 attgtcttta ttggttatcc attctctatc atgctgatta gatataaaac ccatttctat
    31561 caatatagaa ggcatagtag ctttataaag cacaaaaaat ggagcttgtt taatacctct
    31621 attagtaaat ggagctattt gtaaagtgct attggtaacc ttatcagcca aactaatact
    31681 tttgtttaaa aactgatttt gatataatga aaaagcgata tatccttcga tagaattagg
    31741 atctatgcca tcataagatt ctaaataatt ctcttcgtat atggctactg aattttctgc
    31801 tatagcagtt tctagccagg ctttcacttt ggtatttccc attacaaaaa cttcatatcc
    31861 attagcttct atagatttcg aagcatttac atgaaaagat acaaatagat tagcgtgact
    31921 tttattagct atgcgagtcc tttcgattaa aggtataaaa atatcagatt gtctggtgta
    31981 aatgactttt atattttcat aagtattact taaaatattt cctacttcca ataccaaaga
    32041 taatgtaata tctttttcct ggatattacc atatacagcg ccgggatctt tgcctccatg
    32101 gccagcgtct aaaaccaaaa tatatttttg acaaaaagta ttagagcata taaaaataaa
    32161 tattataaaa gtcaactttt tcattttcaa tttattagac aatatgtttt ttgtaataca
    32221 aatttatgca ttatttttaa tttattgtaa aaaaaattta aaatgtcaca tattataaaa
    32281 aggtttttat aatattttta tgttaaattt aatattggct tagctctcaa gtccctgtat
    32341 aaaaaccac
//
LOCUS       DAOPRN010000018        31922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1095_length_31922_cov_117.258951, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000018 DAOPRN010000000
VERSION     DAOPRN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1095_length_31922_cov_117.258951"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PKI65_03405"
     CDS             complement(<1..795)
                     /locus_tag="PKI65_03405"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77523.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEIVTSHRQKRGIYSLSTVKKKAS"
     gene            1116..2576
                     /locus_tag="PKI65_03410"
     CDS             1116..2576
                     /locus_tag="PKI65_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOB77524.1"
                     /translation="MKTILDLEPKRVWHFFHDLTQIPRPSGKEEKIIEYLVNVAKENN
                     LEVIVDDIGNVIMRRAASPGLENRKGIILQAHMDMVPQKNLDKQFDFEKDPIETIIDG
                     EWVRANGTTLGADNGIGLAAALAVLTDKNIKTGMIEGLFTVDEERGMTGAFGLKPDIL
                     KGDVLLNLDSEHDGEIFIGCAGGMDANITFHLSYEPVPDTYKAFEVEIKGLKGGHSGL
                     DIHLGRLNANKALNRFIKFIIDHLDALLVSFDGGSLRNAIPREAKAIILVPDNKINEL
                     YEIKSVFDKNFQIEFGKVEPNLEFNLKETQLPARYIKKDIADKIVKAIYGCPNGVLRM
                     SAEVEGLVETSNNLAVIKTSDDTLSIQCLIRSSIDSAKEAVGEKIKSVFELADADVSL
                     TGAYPGWKPNPDSEILRLAKKIYEEKFGKTPVIKAVHAGLECGLLGSKYPNLDMISFG
                     PTIVAPHSPDERVNIPSVQKFYDFLLEIIKNIPDKN"
     gene            2591..2971
                     /locus_tag="PKI65_03415"
     CDS             2591..2971
                     /locus_tag="PKI65_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOB77525.1"
                     /translation="MSVYSKNHKHHQIWQNSVDLAKDIYELTRELPIDIEYDFVSQMR
                     YCVDTIHSKIVEGVMRYYTNDYPKLLSDCLKYCSRLEKLIIILKDLQYISEITAKNRI
                     EKINNISQMLNNLIRDVNMFYSAN"
     gene            3223..4149
                     /locus_tag="PKI65_03420"
     CDS             3223..4149
                     /locus_tag="PKI65_03420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB77526.1"
                     /translation="MLSIILPTKKRVEIFYETIECLDKTLKLFDHDYEVLVINNDPDP
                     ISFNLPDYVKIINCDGRGVASARNMGAHNAKGDILLFLDNDILISYEAFIKGYKLFLK
                     LIKQNENIAINFNWIYPRDVYDLLEKNSFGRYLLYYEFDKYEGKAWTNRNFDKKVEYQ
                     KTDVFTSSTFFISKSTFNKIGGYTEDFSFAGYEDHNFREKLLKNNIDIYILTNTYVFH
                     NERDYIDLNKFLERKYRSAFTRKQAVSLGDNELILEYSYLKKILLTIIWPLRNILIYF
                     VNNFSQSKKFDPLMFKIIDTLLAVYIYKGYNN"
     gene            4159..5268
                     /locus_tag="PKI65_03425"
     CDS             4159..5268
                     /locus_tag="PKI65_03425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOB77527.1"
                     /translation="MRKKVVYIISDIDKAFEFEWAATYLSPYFDLHFILLNPSDSFLE
                     KFLIDYGIPVYRIKYINKRDYISAFKSVYKLLKRIKPDIVHCHLIVANLIGLPAAKLA
                     GVKNRIYTRHHSSYHHIYHKHAIIYDKISNSLATDIVAISPIVKEILTKWEKVSDSKV
                     KIIPHGLPNDLFDNVTYEKIENLKSKYNLEGYSPIIGVISRFTEWKGVQYIIPAFQRL
                     LKDFPKAKLVLANAIGDYENEINKLLAELPLGSYEKIFFEPDMATLFKTFDVFVHVPI
                     DNHSEAFGQVYIEALAGGIPMVCTRSGIANECVIHLENAYVVDYKDCDSIYLGLITLL
                     ENNDLKEKLMDNAPKSVTNYTVDNKFNLLRDLYNE"
     gene            5261..6136
                     /locus_tag="PKI65_03430"
     CDS             5261..6136
                     /locus_tag="PKI65_03430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB77528.1"
                     /translation="MNKNISNPLVSIIIPVYNGEKYIAETIQSVIDQTYENWELIIVD
                     DGSTDNTSNIVKKFNDTRIIYIKKNNTGVSDTRNIGAKMSKGNFLCFLDADDVWLSKN
                     LEIKVKKLLDEPDCVLVSSAFYLWDERDDYINCKINDYVPNDDWEDLRKLRSISLPSS
                     ALISSKFFFEANMFDTNLSTSADFDLWIRMRALGHFNFISEPLVKYRSHSNQMHRNIK
                     LYEHDMIYILKKYKDCKKIFPTRNSWKKSMSLLHYYLAVEYVRQKQINNFFVHSTKSI
                     FFNPSLIFYKMIKKI"
     gene            6456..6839
                     /gene="rpsL"
                     /locus_tag="PKI65_03435"
     CDS             6456..6839
                     /gene="rpsL"
                     /locus_tag="PKI65_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOB77529.1"
                     /translation="MPTIQQLIRKGRKAKRKASKSPALENCPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGYEVIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGAL
                     DTAGVENRKQSRSKYGTKRPKQKQN"
     gene            6846..7313
                     /gene="rpsG"
                     /locus_tag="PKI65_03440"
     CDS             6846..7313
                     /gene="rpsG"
                     /locus_tag="PKI65_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOB77530.1"
                     /translation="MRKTKAKQRIILPDPKYQDEIVAKFVNNLMLRGKKEIAYKIFYE
                     AIDIVTKRTNEDGLEVFKKALENVTPQLEVRSRRIGGATFQIPTEIRPERKLSIGMKN
                     LISHARKRNDKGMPTKLANEIIAASKQEGGAFKRKEEIHRMAEANKAFAHFRF"
     gene            7336..9441
                     /gene="fusA"
                     /locus_tag="PKI65_03445"
     CDS             7336..9441
                     /gene="fusA"
                     /locus_tag="PKI65_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOB77531.1"
                     /translation="MKDLTPLRNLGIIAHIDAGKTTTTERILYYTGVNYSIGEVDDGT
                     ATTDWMPQEQERGITITSAATTVHWKLNDNDYQINIIDTPGHVDFTVEVERSLRVLDG
                     CIVIFCGVAGVQPQSETVWRQADKYNVPRICYINKLDRVGADFFKVVNQIKNKLGANP
                     IPIQIPIYNDDKFDGIIDLIYQKAIYWDLLNAPDGSLLIYKDIPDNFKVIVDKYRAIL
                     LEAVAELDDVIMDKYFNAPDSITEKEIISTLRKGTLSSKFTPILCGASLQNIGVQPLI
                     DAAVNFLPSPADIGYVKGINPKTGEEITRKISDDEPLSALIFKVVNDPYVGRIAFTRI
                     YSGSISQGEMLYNSRLNKKERAARLFRIHSNKQIKIDSFSAGDIAAIIGLRESITGDT
                     LCDPSHPITFESFYFPDPVISIAIEAKYQKDFTKMLESLQKLADEDPSLLIKTDEESG
                     QIILNGMGELHLEIIADRLRREYEIECNLGKPQVNYREGIIGKTIHREIYKKQFGGKG
                     KYAELEFELSGEPFDKIGLTFENIVGNSLTKDYLRSIEKGFKSAMLNGILAGYTVLNA
                     NIRLLDIVTHPVDSDPLAFEIAANIAFKEACKKLKIVLLEPIMKLEIISPSEYIGDIS
                     SDITRRRGEILQIDSQESYQIIRAEAPLSELFGYVTALRSLSSGRASVNMEFARYDVC
                     PDVIAEEVIFKTKGIKVNL"
     gene            9452..9757
                     /gene="rpsJ"
                     /locus_tag="PKI65_03450"
     CDS             9452..9757
                     /gene="rpsJ"
                     /locus_tag="PKI65_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOB77532.1"
                     /translation="MVNRIRIKLKSYDHYLIDKSAEKIIKAVKATKAVINGPIPLPTE
                     KKIFTVNRSTFVHKESREQFQLATHKRLIDIYFTDQSTIDELMKLELPRGVEIEIKV"
     gene            9777..10409
                     /gene="rplC"
                     /locus_tag="PKI65_03455"
     CDS             9777..10409
                     /gene="rplC"
                     /locus_tag="PKI65_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOB77533.1"
                     /translation="MSGLLGKKIGMTSIYDQNGKRVAVTVIEAGPCIVTQIKTKANDG
                     YDAIQLAFDDIDERKVTKPLLGHFKKTNTQPKRILKEFTRFEEGFQKQLGEVLTPDKI
                     FVEGEYIDVSGITKGKGFQGVVKRHGFSGVGMGTHGQKDRQRHAGSIGASSYPSRVMK
                     GTKMPGRDGGKKVKMINLQIMKIIPEKNLVLVKGAVPGHVGSYLKLERWK"
     gene            10400..11041
                     /gene="rplD"
                     /locus_tag="PKI65_03460"
     CDS             10400..11041
                     /gene="rplD"
                     /locus_tag="PKI65_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOB77534.1"
                     /translation="MEVKILKQDGSETSRTAILNDEIFNIQPNDHAIWLDVKRIQANK
                     RRGLASTKERNQVKGSRRKLYRQKGTGRARKGDIKSPILRGGGVVFGPHPRDYSIKVN
                     KKVKVLARKSALSYKAQNNQIIVIDDMQFDEVKTKKFKEILANLNIENQKILFVLDKP
                     NYFVFLSAKNLQKVKVTLASQLNTYDILNANLIVFNEASLPIVEEILLRFQKV"
     gene            11047..11349
                     /gene="rplW"
                     /locus_tag="PKI65_03465"
     CDS             11047..11349
                     /gene="rplW"
                     /locus_tag="PKI65_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOB77535.1"
                     /translation="MEPKDIIIKPQITEKATLQTEKFANRYTFIVHQKANKYQIKKAV
                     EWLYKVEVDRVNTMNFQGKKKTRGTRTGWIEGRKPSYKKAVVFLKEGYSIDFYSNI"
     gene            11363..12187
                     /gene="rplB"
                     /locus_tag="PKI65_03470"
     CDS             11363..12187
                     /gene="rplB"
                     /locus_tag="PKI65_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOB77536.1"
                     /translation="MGLKKFKPVTPGLRFKVINDFSEITTDKPEKSLLVPIKKTGGRN
                     NEGHRTIRYRGGGHKRMYRVIDFKRNKDGIPAKVRSIEYDPNRSARIALLVYADGEKR
                     YIIAPQGLKVGQTVMSGRGIAPEIGNCMYLSEIPLGSLIYNIEMRPNEGGKIARSAGS
                     YAQLLSRDNKYAVIKLPSGETRLIPVNCKATIGMCSNPEHSLIRHGKAGRRRWLGRRP
                     RTRGVAMNPVDHPMGGGEGKASGGHPRSRNGIYAKGQKTRKKNKPSNQFIIERRKK"
     gene            12197..12478
                     /gene="rpsS"
                     /locus_tag="PKI65_03475"
     CDS             12197..12478
                     /gene="rpsS"
                     /locus_tag="PKI65_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOB77537.1"
                     /translation="MSRSLKKGPYVHYKLEKKVLEMQRAPRKQVIKTYSRASMITPDF
                     VGLTIAVHNGKTFIPVYITENMVGHKLGEFAPTRVFRGHAGHRKEDKRK"
     gene            12518..12955
                     /gene="rplV"
                     /locus_tag="PKI65_03480"
     CDS             12518..12955
                     /gene="rplV"
                     /locus_tag="PKI65_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOB77538.1"
                     /translation="MAEEIKNKKSQTYFAKLNKCPMSARKMRPIANLVRGKSIEDALV
                     ILKTTPKKGARFLYKLLLHNIDNYKQKSKSGLRIEEAQLYIKTLMVNQGPALKRYRPA
                     ARGSAHPIRKESCHIYMELASRVEEMSANINNSEVESFNNTEV"
     gene            12955..13581
                     /gene="rpsC"
                     /locus_tag="PKI65_03485"
                     /pseudo
     CDS             12955..13581
                     /gene="rpsC"
                     /locus_tag="PKI65_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            13694..14113
                     /gene="rplP"
                     /locus_tag="PKI65_03490"
     CDS             13694..14113
                     /gene="rplP"
                     /locus_tag="PKI65_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOB77539.1"
                     /translation="MLQPKKTKYRKVQKGRMKGNANRGTQLAFGSFGLKTLEPAWITA
                     RQIEAARQTATRYMKRQGQLWIRIFPDKPVTKKPNEVRMGKGKGNPEYFVAPVLPGRI
                     LFEVDGVPLDIAKEALRLAAQKLPVKTKFIISNDYIE"
     gene            14139..14360
                     /gene="rpmC"
                     /locus_tag="PKI65_03495"
     CDS             14139..14360
                     /gene="rpmC"
                     /locus_tag="PKI65_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOB77540.1"
                     /translation="MEISVIRELSTNELKERLDDLKHEYAILKMNHAVSPVDNTAKFR
                     EIRKDIARILTELTEREKKEKSENIVKSE"
     gene            14363..14626
                     /gene="rpsQ"
                     /locus_tag="PKI65_03500"
     CDS             14363..14626
                     /gene="rpsQ"
                     /locus_tag="PKI65_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOB77541.1"
                     /translation="MEKINKKNRKERTGIVVSNKMDKSVVVRINYHKMHPLYKKYVKK
                     YKKFMAHDENNECNIGDKVRIVETRPLSKHKHWRVVEIIERGK"
     gene            14629..14997
                     /gene="rplN"
                     /locus_tag="PKI65_03505"
     CDS             14629..14997
                     /gene="rplN"
                     /locus_tag="PKI65_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOB77542.1"
                     /translation="MIQQETRLKVADNSGAKEVLCIRVLGGTGKRYATVGDKIIVTVK
                     SALPSGNIKKGTVTKAVVVRTKKEIRRDDGSYIRFDDNACVLLSPTDELRATRIFGPV
                     PRELREKQYMKIVSLAPEVI"
     gene            15031..15378
                     /gene="rplX"
                     /locus_tag="PKI65_03510"
     CDS             15031..15378
                     /gene="rplX"
                     /locus_tag="PKI65_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOB77543.1"
                     /translation="MAKTRKNKSEKPVKLKIKKGDYVRVIAGDDRGAEGRVLKVLPKE
                     QRVIVEGVNIIIKHTKPSAAHPQGGRIEVEAPIHISNVMLLVGDTPTRIGKKINENGK
                     LVRYAKKTGEVIK"
     gene            15375..15935
                     /gene="rplE"
                     /locus_tag="PKI65_03515"
     CDS             15375..15935
                     /gene="rplE"
                     /locus_tag="PKI65_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOB77544.1"
                     /translation="MTTTTYIPRLKKKYYDDVVPAMMKEFEYKNIMQVPKIEKIVINQ
                     GIGEATQDRKLIDAAIQELSLITGQKAVPTFAKRDINNFKLRRGNPIGAKVTLRGDMM
                     YEFLDRLISIAIPRIRDFHGISDNGFDGRGNFSFGISEQIIFPEIVIDKINKIIGMNI
                     TIVTTARNDKEAYSLLKNIGIPFKKH"
     gene            15961..16230
                     /gene="rpsN"
                     /locus_tag="PKI65_03520"
     CDS             15961..16230
                     /gene="rpsN"
                     /locus_tag="PKI65_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOB77545.1"
                     /translation="MAKESIKARERKRRQMVEKYADKRAELKAKGDYVALQKLPRNAS
                     PVRLRNRCAISGRPRGYIRYFGISRIEFRQLASKGLIPGVKKASW"
     gene            16399..16794
                     /gene="rpsH"
                     /locus_tag="PKI65_03525"
     CDS             16399..16794
                     /gene="rpsH"
                     /locus_tag="PKI65_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOB77546.1"
                     /translation="MTDPIADYLTRIRNAIRAKHRIVEIPASNMLISITHILKDEGYI
                     LNYKVDEESLPRKLIIALKYHPTMKISPIQNLVRVSKPGRRIYKKADELPKVMNGLGI
                     AIISTSEGIMTDKEARKRHIGGEVVCYVS"
     gene            16815..17375
                     /gene="rplF"
                     /locus_tag="PKI65_03530"
     CDS             16815..17375
                     /gene="rplF"
                     /locus_tag="PKI65_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOB77547.1"
                     /translation="MSRIGKMPIKIPQGVSVEVSKDNIVRVKGKLGQLEQYVQPSIKI
                     NIDGDIMTLTRINDEKQTRAYHGLYRSLINNMIIGVSEGFTKTLELVGVGYRAQAQGQ
                     LLELSLGFSHNIIFELPPEISVTTITERGSNPKIILKSFDKQLLGQVAAKIRSLRKPE
                     PYKGKGIRYEGEYVRRKAGKAAASIK"
     gene            17403..17759
                     /gene="rplR"
                     /locus_tag="PKI65_03535"
     CDS             17403..17759
                     /gene="rplR"
                     /locus_tag="PKI65_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOB77548.1"
                     /translation="MKNKKEARRKRIKMRIRKKIRGTTDKPRLCVFRSDKEIYAQIID
                     DSQNITIMAVSSLCKELKDLKMTKTDKAREVGKLIAQKALDADIKTVIFDRNGYLYHG
                     RVKALAEGAREKGLKF"
     gene            17765..18280
                     /gene="rpsE"
                     /locus_tag="PKI65_03540"
     CDS             17765..18280
                     /gene="rpsE"
                     /locus_tag="PKI65_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOB77549.1"
                     /translation="MANIVRKVKAADAELKDRLVSIKRVTKVTKGGRTFSFTALVVVG
                     NEDGIVGCGLGKAREVPLAIQKAIDDAKKNLVKVSIRNGTIPHEQTTKYGAAKVIIKP
                     AAPGTGVIAGGAMRAVLESAGVKNVLAKCIGSSNPHNVVKATIKALTELRDPYMIADL
                     RNIPLEKVIGK"
     gene            18295..18474
                     /gene="rpmD"
                     /locus_tag="PKI65_03545"
     CDS             18295..18474
                     /gene="rpmD"
                     /locus_tag="PKI65_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOB77550.1"
                     /translation="MKQIKVTLVRSLIGRPKDQKRTAEALGLRKLNHSRIFDDTPSIR
                     GMIKKIEHLVKVEDI"
     gene            18487..18936
                     /gene="rplO"
                     /locus_tag="PKI65_03550"
     CDS             18487..18936
                     /gene="rplO"
                     /locus_tag="PKI65_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOB77551.1"
                     /translation="MDLSSLKPAKGSVKSKTRVGRGEGSKKGGTAGRGTKGQKSRSGY
                     SRKIGFEGGQMPLQRRVPKFGFKNIFKETYKPINLDILQDFAEKQNITIINPDVLYEY
                     GLISKKDKVKILARGELKLTLEVSAHAFSKKAKEEIEKLGGNVKIIN"
     gene            18936..20297
                     /gene="secY"
                     /locus_tag="PKI65_03555"
     CDS             18936..20297
                     /gene="secY"
                     /locus_tag="PKI65_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOB77552.1"
                     /translation="MSNFIQTFKNIFKIEDLKKRILYTLLLVFIYRFGTHVVLPGVNA
                     SLIKGSTIAQQGQGGLLGMINMFAGGAFSNASILALGIMPYISASIIIQLLTVVIPSF
                     QRMQKEGESGRRRLNQITRYLTIVVTVAQAQGYITYLATQLPRQAIYPFTSAGSIPFI
                     FRFSTTILLTAGTLFVMWLGEKITDKGIGNGISLIIMVGIVARLPVALLSEFGSRMSV
                     AGGGIFALIIEIILLIGVILFTILLIQGTRKIPVQYAKRVVGNRQYGGVRQYLPLKVN
                     AAGVMPIIFAQAIMFLPITIAGFANYESGGWFVTAFSNFNGFWYNLTFALLIIVFTYF
                     YTAITFNPQQIADELKKNNGFIPGIKPGKKTAEYIDTIMSRITLPGSIFLAIIAILPA
                     IVSNFGITSQFAQFYGGTSLLILVGVVMDTLQQIESHLLMRHYDGFMKKGKLKGRSVY
                     GGI"
     gene            20300..21073
                     /gene="map"
                     /locus_tag="PKI65_03560"
     CDS             20300..21073
                     /gene="map"
                     /locus_tag="PKI65_03560"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOB77553.1"
                     /translation="MIIRDVEELELIKKSADLVGKTLGEISKILKPGIIASEIDKIAE
                     EFIRDNGGIPAFKNYNGYPSTLCISINDVVVHGIPKNQVIKEGDIVSIDCGAFLNGYC
                     GDYAYTFMIEPVDEKIRKLVITTYEALYKGIEQAVVGNRIGDIGFAIQLYVERNGFSV
                     VRELVGHGIGKTMHEYPQIPNYGKKGKGYLLEDGMVICIEPMINMGKKEVIFGNDGWT
                     VLTKDHKPSAHFEHMIWINQKKPVILSTYNYIDEKWKVK"
     gene            21076..21291
                     /gene="infA"
                     /locus_tag="PKI65_03565"
     CDS             21076..21291
                     /gene="infA"
                     /locus_tag="PKI65_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOB77554.1"
                     /translation="MPKQNVIEKDGIVRESLGNAMFRVELDNGHVILAHISGKMRMHY
                     IRILPGDKVRVEMSPYDLTRGRIVYRY"
     gene            21308..21424
                     /gene="ykgO"
                     /locus_tag="PKI65_03570"
     CDS             21308..21424
                     /gene="ykgO"
                     /locus_tag="PKI65_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOB77555.1"
                     /translation="MKVRASVKKRTPDDKIVRRKGRVYIINKKNPKYKQRQG"
     gene            21449..21829
                     /gene="rpsM"
                     /locus_tag="PKI65_03575"
     CDS             21449..21829
                     /gene="rpsM"
                     /locus_tag="PKI65_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOB77556.1"
                     /translation="MARIVGVDLPKNKHGEIALTYIYGIGRSTARYILSKADVDFDKK
                     VKDWTDDEISKIRQIINDEIKTEGALRSEVQMNIKRLVDINCYRGMRHRLGLPVRGQR
                     TRTNARTRKGRRKTVAGKKKAPRT"
     gene            21854..22249
                     /gene="rpsK"
                     /locus_tag="PKI65_03580"
     CDS             21854..22249
                     /gene="rpsK"
                     /locus_tag="PKI65_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOB77557.1"
                     /translation="MAKTRSRTASKKRVVKVEPFGCAYIRATFNNVIVTLTNEKGDVI
                     SWSSAGKMGFRGSKKNTPYAAQVAAEDAAKTALDAGLKKVKVYVKGPGAGRESAIRTI
                     HNAGIEVTTIIDITPVPHNGCRPPKRRKV"
     gene            22273..22878
                     /gene="rpsD"
                     /locus_tag="PKI65_03585"
     CDS             22273..22878
                     /gene="rpsD"
                     /locus_tag="PKI65_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOB77558.1"
                     /translation="MARYIGPKTKVARSLGSPVYGYDKYYERKNYPPGMHGLSRRRRK
                     PSEYRMQLLEKQKAKYTYGLKEAQFRRFYSIALSKGGITGENLMQLLESRLDNVVYRL
                     GIAKTRPAARQLVSHKHIIVNDRVVNIPSYLVKPGDIIGVRERSKSLEIIQEAITGRV
                     DKYSWLQWDQSNMAGTFLQYPVRTEIPENINEQLIVELYSK"
     gene            22885..23889
                     /locus_tag="PKI65_03590"
     CDS             22885..23889
                     /locus_tag="PKI65_03590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOB77559.1"
                     /translation="MAILDFQKPDQVIISENTETYGVYELQPLEPGYGITIGNALRRV
                     LLSSLEGYAITQFSINNMPHEYTSIDGVVEDIIYIGLNLKQVRLKPNFDNVENESFSI
                     IISGQDEFKAGDLNKILTSFTVINPDLHICTMDPSVELNIEFKIQKGRGYVLAEELKD
                     PEAPIGTITLDAVFTPIKNVAYYIENFRVGQKTDYEKLILEINTDGTVSPYMALNEAA
                     NILLQHIEIFCEKKYSLQNDLDQLDDAKDDDVDENYEQMRKLLQTKLSDLDLSVRAIN
                     CLKAADIETLGQLVSYSKNDLLKFRNFGKKSLSEVEEVVKSKNLYFNMDISKYNLDKE
                     "
     gene            23894..>24576
                     /gene="rplQ"
                     /locus_tag="PKI65_03595"
     CDS             23894..>24576
                     /gene="rplQ"
                     /locus_tag="PKI65_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOB77560.1"
                     /translation="MRHRNKINHLGRTHTHHKALMRNLSIALIKHKRINTTVPKAKEL
                     RQFIEPIITLTKNDTTANRRLAFSKLQNKEAVKILFDEIAEMVADRPGGYTRILKTGY
                     RVGDGAHMCIIELVDFNPEYSQARSTKAATKPKTRRGGGRGKKVSQQPDIKEDTKAET
                     KPITESEIEFDKEDAKAEADVKEDTKAKTKPVKESEIESDKEDAKAEPDIKEDTKAKT
                     KPVKESEIES"
     assembly_gap    24577..24676
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            24952..26232
                     /locus_tag="PKI65_03600"
     CDS             24952..26232
                     /locus_tag="PKI65_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOB77561.1"
                     /translation="MLLSVVGYPIAHSLSPILFEYLSYKYHISIHYSRLAIDNLDDFN
                     KIFNENLDVFDGFNITYPLKKDVYHMCQHYSNIPTGIHCCNTIVCKPSISIYNTDYHA
                     IKYILSREVKNLWEQVIILGSGMTASICAWALINYYKPIIIFYHKNINYEYFPKNKNV
                     HWKQLNKINESINDKTLLINTLSPMANYEFLPKKLNAVTTIIDTDYRNLPLKNVAISN
                     NINYIDGYHWLYYQGIEAFKIFTNKTNLSVEYNEEWIKINKYDKIALIGMSGAGKTTI
                     GQKLAKKLNYQFVDIDEIIEKQEHKTISEIFADDGEDFFRQLETQMLAKIIKNEKIII
                     ATGGGIIESEKNRKLLKSNFFNILLYVNVDEAINRLNNSKTRPLLTGNIKGNWEKLWN
                     DRRLLYYETADYIINLAKNNEDLIYEDIKEIIKK"
     gene            26201..27415
                     /locus_tag="PKI65_03605"
     CDS             26201..27415
                     /locus_tag="PKI65_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77562.1"
                     /translation="MKISRKLLKNNLSISLLGSKSIVIRHLLISLFHNSKIILRNVQF
                     CDDVVVAINLIKTYGKIVDIVDKNTIKISGQITKIPEKIYCGESASLLRMIVPIHHSF
                     HNKCRYDGDKTLLVRDFIDIEEMLSSLGLSFSSTKLPFIVIGELNSQNNYSLMAKQSS
                     QVVSGLLISTCFQNLVTTIHCPELVSAPYVYLTAEIINLYGGMITYNDKTFQVKPNNL
                     QEKSNDFLIEGDWSLGAMILALGLTFESCTVKNLSSNSLQPDSEILSLFDNLSIKYQV
                     TENSIITYKQNYNGFSYDITNTPDLVPALMILSANANSPSVITGIKRLTNKESNRANT
                     LAENFIRLGGRLETTNENTWIIYPSQIHGGEIKTYEDHRVAMLGIFLGLLSEEDVEID
                     NWQCISKSYWIK"
     gene            27664..28086
                     /locus_tag="PKI65_03610"
     CDS             27664..28086
                     /locus_tag="PKI65_03610"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOB77563.1"
                     /translation="MKHNLLFIIIFLSTLFINKSFASDNTRLFPGDPIIDKIIACLKT
                     QDARCVGQYFANNIEMTLLSSKGNYSRSQAVALLDNFFVKHPLKSISNVKSGELSDNK
                     IYVVGNYNSGSITYKIYFILFKEINQIAPQILIFNITK"
     gene            28124..28705
                     /locus_tag="PKI65_03615"
     CDS             28124..28705
                     /locus_tag="PKI65_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77564.1"
                     /translation="MEENVRPQNNENVENQDKNVNEQPKKKSWFKRNVGATIIMSLML
                     IVIIYLGINLMLKQKHHREELENQRAQYTSQIDSLKKENYTWLTKVFSWSIRSEWMRN
                     NLEQVLWYSEDLLKFGDINNIKLVDPSGRVIFSTNMKDQNVITEWSNIQKIEIQEKSD
                     KLVVASPIMGTDMPMGAVVIEYKINKQINDTEN"
     gene            28770..30650
                     /gene="mnmG"
                     /locus_tag="PKI65_03620"
     CDS             28770..30650
                     /gene="mnmG"
                     /locus_tag="PKI65_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOB77565.1"
                     /translation="MFENIDIVVIGAGHAGCEAAVVSARLGSKVLLITLDIANPARLS
                     CNPSIGGIAKGQIVKEIDALGGCTGIVADRSMIQFRMLNRSKGPAMWSPRSQNDRWLF
                     SYFWREMLENTPNLYLWKDEAIDILPKNSHIVVKTKMGIEIKAKAVIVTAGTFLNGKI
                     YIGPLQIAGGRLGEPAAVGLTESLKKLNIESGRLKTGTPVRLDGRTIDFSKMIEQPGD
                     EKPSKFSYSDDTKPLEKQRSCWITYTNEKTHDILQKGFHLSPLFTGIIKGVGPRYCPS
                     IEDKIVRFSNKNSHQLFVEPEGWNTYEYYLSGFSSSLPLDIQYEALHTIKGLENAHII
                     QPGYAIEYDYFPPNQLKNTLESRIVENLYFAGQVNGTTGYEEAAAQGIIAGVNAHQKI
                     NNKQPLILSRSQAYIGVLIDDLITKTLDEPYRMFTSRAEHRLILRQDNADLRLSPIAY
                     ELGLIDKERYKKVEQKEQFINQTIKYLSSKSVDPEKINDYLISINSSPLKQKIKWTQL
                     LMRPEISLQDLANNIDEINNLIKDKPLHFIETLEIEIKYAEYINKENELAKKLMKFDE
                     MHLPTDFNYQELKNISIEARQKLNNVKPANIGMASRITGISPADIALIINYFYKKKSN
                     FN"
     gene            complement(30843..31703)
                     /gene="prmC"
                     /locus_tag="PKI65_03625"
     CDS             complement(30843..31703)
                     /gene="prmC"
                     /locus_tag="PKI65_03625"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOB77566.1"
                     /translation="MNLYQAKKILKKAIPEYFYHLSVEPLIRDIITHILNINTIDYLL
                     LPPKYELNNDEINKLFEIIKQLQNEKPLQYILGKAQFLNFELKINENVLIPRPETEEL
                     VKLVINNLEKQKYKPKTIVDIGTGTGCIALAMKYYYPYSRVIGIDISSEAIKLAKFNA
                     IKYNLTIDFFQLDILNTDLSKFKFDVIISNPPYVLNIEKNEIKPNVKNWEPHDALFVF
                     DEDPIIFYKKIINDFFKSPDARFAFFEINPICYNVITDFLSNYTQISYFFEKDFNNKT
                     RYLAIDLLDK"
BASE COUNT        12038 a         4106 c         5437 g        10241 t
ORIGIN      
        1 actagctttc ttcttgacag tacttaggct atatatacct cgcttttgtc tatggctagt
       61 tactatttct ttatgcttga ttacatttct tctaaccttt ttgaatgctt ttttattact
      121 gctacacatg actccatcct ttggcatttt agccttcaga aattgctcta tctgttctga
      181 acttagcttt ttgccatcaa attttttgaa taataagttg ccagctctgt ctgtagcagc
      241 caatacggct actttctttg gcttttgctt cctagctcgc ttcagctctt cggcattttt
      301 gtatattcta cctttttcgg aataatccat caatagttca tccatctcta ctactccgaa
      361 aaaatttata ttatcttcga aactttgtag agctgccaat atcttgtgtc gccaagcgaa
      421 gcttgttggt aggctgatgc ccacttcttt agcacaagct ctgagacttt tgcctgctac
      481 catacatctg atgtagtcga gcattttctc tttcttttgt aacctgtaaa caaaagtacc
      541 tgtggtaact ctaaactgtc tgccacaatc tttgcagcga tagttttgca tcccattttg
      601 taaaccattt ttaataatgt gattgctacg acagtctggg cacagtggcg gggcgctgag
      661 tattttcaga ccttgataat cgactacatc ggcttctaat tttttcatta gagaataaag
      721 gttgtctaag aattctttat tctcttcctt actcatagca aggtatttac tggtgttttc
      781 cactattttt ttcatgaata aaaatttttg taaaaatata aaaattattt aacatataaa
      841 cttgaaaaac ttaaacattt attaattccg attacttaat tatctttgtt tattttatta
      901 tcctccactc acatctattt tatcatactt aaaattattt catatgattt attaaacatt
      961 tataaatata aattgcctct aatatcgtat aaggtcataa ctttttataa ttttttcatt
     1021 tgtatctcat tttgttttgt aattaagcat aaactttaac ttttaattat tttatttcat
     1081 tttttattta tatttttgac aaaaaatata aaattatgaa aacaatttta gatttagaac
     1141 ccaaaagagt gtggcatttt tttcatgatc tcacgcaaat acctcgtcca tcaggaaaag
     1201 aggaaaaaat catagaatat ttagtaaatg tagctaaaga aaacaactta gaagttatag
     1261 tagacgatat cggcaatgta ataatgagac gagcagcttc tccaggctta gaaaatcgta
     1321 agggtattat tctacaagct catatggata tggtaccaca aaaaaactta gataaacaat
     1381 ttgattttga aaaagatcca atagaaacta taatagatgg tgaatgggtc agagctaatg
     1441 gtactacgtt aggtgctgat aatgggattg gtttagctgc tgctttagcc gtacttacag
     1501 ataaaaatat aaaaaccggt atgatagaag gacttttcac tgtcgatgag gaaagaggaa
     1561 tgactggagc ttttggctta aaaccagata ttttaaaagg tgatgtttta ctcaaccttg
     1621 atagtgaaca tgatggtgaa atatttattg gttgtgcagg cggtatggac gctaatataa
     1681 cttttcattt atcttatgag cctgttcctg atacttataa agcttttgaa gttgaaatca
     1741 aaggactcaa aggtggacac agcggattgg atattcatct cggaagattg aatgcaaaca
     1801 aagctctcaa tagatttata aaatttatta ttgatcactt agatgctctt ttagtatctt
     1861 ttgatggtgg ctcattgcgt aatgccatac ctagagaggc taaagctatt atcctagtac
     1921 ctgataataa aataaatgaa ttatatgaga ttaaatcagt ttttgataaa aattttcaaa
     1981 ttgaatttgg caaagtagaa ccaaatcttg aattcaattt gaaagaaaca cagttgccag
     2041 ctagatatat aaaaaaagat attgctgata aaattgtaaa ggctatatat ggttgcccta
     2101 atggcgtttt gagaatgagt gcagaagtag agggcttagt agagacttct aataatcttg
     2161 ctgttatcaa aactagtgat gatactttat ctatacaatg ccttattaga tctagcattg
     2221 atagtgctaa agaagctgta ggtgaaaaaa ttaaaagtgt ttttgaatta gctgatgcag
     2281 atgtttcact tacaggtgct tatcctggtt ggaaaccaaa tcctgatagc gaaatcctta
     2341 gattagctaa aaaaatatat gaagaaaaat ttggcaaaac tccagtgata aaagctgtac
     2401 acgcaggact ggaatgtgga cttcttggta gcaaatatcc taatctcgat atgatatctt
     2461 ttggtcccac tatcgtagcc cctcatagtc ctgatgaacg tgttaatata cctagtgtac
     2521 agaaatttta tgatttttta ttggagatta ttaaaaatat acctgataaa aattaataaa
     2581 aatataaatt ttgagtgtat atagtaaaaa ccataaacat catcaaattt ggcaaaatag
     2641 cgtagattta gctaaagata tctatgagct cactcgtgag ttacctatag atattgaata
     2701 tgattttgtc agtcaaatga gatattgtgt agatactata cactcaaaga tagtagaagg
     2761 tgtgatgaga tattatacta atgattatcc taaattgctt tctgattgtc tcaaatattg
     2821 ctcaagatta gaaaaattaa ttataatact aaaagatttg caatatattt ctgaaataac
     2881 agcaaaaaat agaatagaaa aaattaataa tatctcacaa atgttgaata atctgattag
     2941 agatgtaaat atgttttatt ccgctaatta attttttttt aactggtaaa aaaacaaaaa
     3001 tagtttcaac ttgctcacat ctatttataa atagctagga tgcgtttttg gttgaatttt
     3061 tatactaaat taaattttta ttgatgttca aaatttcaca ttatagcatc ctgtgctttg
     3121 gtgcctcttg cattcttatt gacctcattc gtaaaccttg tcatctatgt aattacaatt
     3181 ttttattact ataatttgta attttttttt gtaattttgt ttatgttaag tataattttg
     3241 cccactaaaa aacgtgtaga aatattctat gaaactatag aatgtttaga taaaaccctt
     3301 aaattatttg atcatgacta tgaagtatta gtaatcaata atgatccaga tccaatatct
     3361 tttaatttac ctgattatgt taaaataatc aattgcgatg gtaggggtgt agcatctgct
     3421 agaaatatgg gcgctcataa tgccaaaggt gatatattgc tttttttaga taatgatatt
     3481 ctaatctctt atgaggcatt tataaaaggc tataaattat ttttaaaatt aattaaacaa
     3541 aatgaaaata ttgctatcaa ttttaattgg atatatcccc gcgatgttta tgatttatta
     3601 gaaaaaaact cttttggtcg atatttatta tattatgaat ttgataaata cgaaggcaaa
     3661 gcatggacta atcgtaactt tgataaaaaa gttgaatatc aaaaaactga tgtgtttaca
     3721 agtagtacct tttttatttc taaatctaca tttaataaaa tcggtggcta tacagaagat
     3781 tttagttttg ctggctacga agatcataat tttagagaaa aacttttaaa aaataatatt
     3841 gatatttata ttttaacaaa tacttatgtt tttcataatg aaagagatta tattgatttg
     3901 aataaatttc ttgagagaaa atatagatct gcttttactc gcaaacaagc agtttcatta
     3961 ggtgataatg aattgatatt ggagtattct tatttaaaaa aaatactttt aactattatt
     4021 tggcctctta gaaatatttt aatttatttt gtaaataatt tttcacagtc taaaaaattt
     4081 gatcctttaa tgtttaaaat cattgataca ttattagctg tttatattta taaaggttat
     4141 aataattaat tttttttaat gagaaaaaaa gtagtatata ttatttcgga cattgataag
     4201 gcatttgaat ttgaatgggc tgctacttat ctttcgccat acttcgattt acattttatc
     4261 ttattaaatc catcagattc ttttttagag aaatttttga tcgactatgg tatacctgtt
     4321 tacagaataa aatatataaa taaaagggat tatatatcag cttttaagag tgtttataaa
     4381 ttattaaaaa gaataaaacc tgatattgta cattgtcatt tgatagtagc caatctaatt
     4441 ggtttaccag cagctaagct agctggtgtc aaaaatcgta tatatacacg tcatcattct
     4501 tcatatcacc atatttatca taaacatgct attatttacg acaaaattag caattcatta
     4561 gctacagata tagttgctat tagtccaata gtaaaagaga tcttaactaa atgggaaaaa
     4621 gtttctgatt ctaaagtgaa aattattcct cacggtttgc caaatgatct attcgataat
     4681 gttacatatg aaaagataga aaatttgaaa tctaaatata atttagaagg ctatagccct
     4741 ataattggtg ttatttctag atttacagaa tggaaagggg tgcaatacat tattcctgca
     4801 tttcaaagat tgttaaaaga ttttccaaaa gctaaattag tattagcaaa tgctatagga
     4861 gactacgaaa atgaaataaa caaattatta gctgagttgc cattaggttc ttatgaaaaa
     4921 atcttttttg agccagatat ggctactctt tttaaaactt ttgatgtttt tgtacacgtt
     4981 cctatagaca atcattccga agcctttgga caagtataca tagaagcctt agcaggtggt
     5041 ataccaatgg tatgtactcg tagtggaata gcaaatgagt gtgtgattca tttagaaaat
     5101 gcttatgtag tggattataa agattgtgat tctatttatt taggtttgat aactttatta
     5161 gaaaataatg atttaaagga gaaattaatg gataatgctc ctaaatcagt aacaaattac
     5221 acagtagata ataagtttaa tttattaaga gatctatata atgaataaaa atatttctaa
     5281 tcctttagtg agcataataa taccagtata taatggtgaa aagtatatag ctgaaactat
     5341 acaaagtgtg atagatcaaa cttatgaaaa ttgggaattg atcatcgtag atgatggatc
     5401 tacagataat accagtaata tcgtaaaaaa atttaatgat acccgcataa tttacataaa
     5461 aaagaataat acaggcgtat ctgatactag aaatatcggt gctaaaatgt ctaagggaaa
     5521 ttttttatgt tttttagatg ctgatgatgt gtggttatca aaaaatttag aaataaaagt
     5581 aaaaaaatta cttgatgagc cagattgtgt tttggtatca tcagctttct atttatggga
     5641 tgagagagat gattacatta attgtaaaat taacgattat gtaccaaatg atgattggga
     5701 agatcttaga aaattacgtt ctatttcttt gccatcatct gcattgatat ctagtaaatt
     5761 tttttttgaa gctaatatgt ttgatactaa tctgagtaca tcagcagatt ttgatctttg
     5821 gatacgaatg agagctttgg ggcattttaa ttttatatct gaacctttag taaaatatcg
     5881 ctctcactcc aaccaaatgc ataggaatat aaagctttat gaacatgata tgatttacat
     5941 tttaaaaaaa tataaggatt gcaaaaaaat atttcctact agaaactcat ggaaaaaatc
     6001 tatgagtcta ttacattatt acctagcagt agaatatgta cgtcaaaagc aaattaataa
     6061 tttttttgtt cattcgacaa aaagtatatt ttttaaccct tcattaattt tttataaaat
     6121 gataaaaaaa atataaaatt tcaaaaaata aggttttttt ttaaaggtat tcagtgttat
     6181 ctatttatat tataataaag ctatttcacc ttttggaaag ttatgatgta tttctaaaaa
     6241 aagaaaagtt tttatttcat tattacaact gttgctacaa gtaacatcat gtgtagtaat
     6301 ctgagcataa ttattaaaat caaacaagag taaaataaag taatgattat ttcttatatt
     6361 taattaattt ttaaaaaaat tgaaaatata ttttgttaat taaataattg tatgtaattt
     6421 tgcaaactaa ttttttgaaa aatataacaa taaatatgcc aacaatacaa cagttgataa
     6481 gaaaaggtag gaaagctaag agaaaggcta gtaaaagtcc agctcttgaa aattgtcctc
     6541 agcgtagagg tgtttgtact cgtgtataca ctactacacc taaaaaacct aattcagctt
     6601 tgagaaaagt tgctaaggtt agattgacca atggatatga agtgatagct tatatcccgg
     6661 gcgaaggaca taatttgcaa gaacatagca ttgttttgat aaggggtggc cgagtaaaag
     6721 atttacctgg tgtccgttat catattgtac gaggtgctct agatactgca ggtgtagaaa
     6781 atagaaaaca aagccgaagt aaatacggta caaaacgtcc aaaacaaaaa caaaattaat
     6841 aagcaatgag aaaaacaaaa gcaaaacaaa gaattatatt acctgatcct aaatatcagg
     6901 atgagatagt agctaaattt gttaataatt taatgcttag aggtaaaaaa gaaatagcat
     6961 ataaaatatt ttatgaagct attgatattg taaccaaacg tactaatgaa gatggtttag
     7021 aagttttcaa aaaagcttta gaaaatgtca caccacaatt agaagttaga agtagacgta
     7081 ttggtggagc tacttttcaa atacctactg agatcagacc tgaaagaaaa ctgagtatcg
     7141 gtatgaaaaa tctcatttct catgctcgta aacgtaatga taaaggtatg ccgactaaat
     7201 tagctaatga aattattgct gctagtaaac aagaaggtgg cgcctttaaa cgtaaagaag
     7261 aaattcatag aatggcagaa gctaacaaag cttttgctca ttttagattt taacaattat
     7321 aaacatttta ataatatgaa agatttaacc cctcttagaa atttaggaat aatagctcac
     7381 atagatgctg gtaaaactac cactactgag cgtatattat actatacggg ggttaattat
     7441 tctattggag aagtagacga tggtacagct actactgatt ggatgccaca agaacaagaa
     7501 cgtggtatta ctattacatc agccgctact acagttcatt ggaaacttaa tgataacgat
     7561 tatcagataa atattataga cactcctggt catgttgatt ttactgtaga agtagagcgt
     7621 tctttaagag ttttggatgg ttgcatagtt attttttgtg gtgtagcagg agtccaacct
     7681 caatctgaaa ccgtatggcg tcaagctgat aaatacaatg ttcctagaat ttgttacatc
     7741 aataaacttg atagagtagg agctgatttt ttcaaagtag ttaatcaaat aaaaaataaa
     7801 ctaggagcta atcctattcc aatacaaata cctatttata atgatgataa atttgacggt
     7861 atcatcgatc ttatctatca aaaagctatt tattgggatt tattaaatgc tcctgatggg
     7921 tctttattaa tttataaaga tattccagac aattttaaag ttatcgttga taaatatcga
     7981 gctattcttt tagaagctgt agcagaatta gatgatgtga taatggataa atattttaat
     8041 gcccctgatt ctattactga aaaagagata atctcaactc ttagaaaagg tactttatca
     8101 tctaagttta ctcctatttt gtgtggggct tctttgcaaa atattggagt gcaaccttta
     8161 atcgatgctg cagttaattt tttgccttca ccagcagata tcggctatgt gaaaggtatt
     8221 aatccaaaaa ctggtgaaga aattactaga aaaatttcag acgatgaacc tttgagtgca
     8281 ttgattttta aagtagttaa tgacccatat gtgggtcgta ttgcttttac tagaatatat
     8341 agtggttcta tttctcaagg ggaaatgctt tataattcta ggttaaataa aaaagaacgt
     8401 gctgctagat tatttcgtat tcattctaat aagcagataa aaattgattc tttctctgca
     8461 ggcgatatag ctgctattat cggattgaga gaatctatta caggagatac tctatgtgat
     8521 ccttctcatc ctatcacatt cgaatctttt tattttccag atccagttat ttctatagct
     8581 atagaagcta aatatcaaaa agattttact aaaatgttag aatctcttca aaaattagct
     8641 gatgaggatc cctctttact gattaaaact gatgaagaaa gcggacaaat tattctaaat
     8701 ggaatgggcg aattgcattt agaaattatt gcagatagac ttagaagaga gtatgagata
     8761 gaatgtaatt taggaaaacc acaggtaaat tatcgagaag gtataattgg taaaactata
     8821 catagagaaa tttataaaaa acaatttggt ggcaagggta agtatgctga attagaattt
     8881 gaacttagtg gtgaaccttt tgataagata ggtctaacat ttgaaaacat tgtcggtaac
     8941 tcactaacaa aagattattt aagaagcatt gaaaaaggtt ttaaatctgc tatgttaaat
     9001 ggtatacttg caggttatac tgttttgaat gctaatattc gtttattaga tatagtaaca
     9061 catccagtgg atagcgatcc attagctttt gaaatagctg ctaatattgc ttttaaagaa
     9121 gcttgtaaaa aattaaaaat tgttttatta gaacctataa tgaagcttga aattatatct
     9181 cctagtgaat atataggtga tataagtagt gatattacga gaaggcgagg tgaaatcttg
     9241 caaatagata gtcaagaatc atatcaaatt attagagccg aagcgccttt atcagagcta
     9301 tttggatatg taactgcatt gagaagtttg agtagtggta gagcttctgt aaatatggag
     9361 tttgctcgtt atgatgtttg ccctgatgtc attgctgaag aagtgatttt taaaacaaaa
     9421 ggtattaaag tgaatttata aaaattaaaa tattgtgaat agaataagaa ttaaattaaa
     9481 atcttacgat cattatctga tagataaatc agctgaaaag atcataaaag ctgtaaaagc
     9541 aacaaaagct gtgatcaatg gaccgatacc attgccaaca gaaaaaaaga tttttacagt
     9601 taatcgttct acttttgtac acaaggaatc tagagagcaa tttcaattag ctactcataa
     9661 aagattgata gatatttatt tcactgatca atcaactatt gatgaattga tgaaattaga
     9721 actaccacgc ggtgtagaaa tagaaattaa agtctaaaaa gctaggaggt aatattatgt
     9781 cgggtttatt aggtaaaaaa ataggaatga ccagtattta tgatcaaaat ggcaaacgtg
     9841 tagctgttac tgtgatagaa gctggtccat gcatagttac tcaaattaaa actaaagcaa
     9901 atgatggata tgatgctata caattagcct ttgatgatat tgatgaaaga aaagttacta
     9961 aaccactatt aggacatttc aaaaaaacca atactcaacc taaaagaata ttgaaagaat
    10021 ttactcgctt cgaagaaggt tttcaaaaac aactaggaga agttttaact ccagataaaa
    10081 tttttgtaga aggtgagtat atagatgtct caggcattac taaaggcaaa ggatttcaag
    10141 gtgttgtgaa acgtcatggt tttagtggtg taggtatggg aacgcatgga caaaaagata
    10201 gacagagaca tgctggttct ataggtgcta gttcatatcc ttctagagtg atgaaaggta
    10261 ctaaaatgcc tggtcgtgat ggaggtaaaa aagttaaaat gattaacctc caaattatga
    10321 aaatcattcc cgaaaaaaat ttggtattag ttaaaggtgc cgttcctggc catgtaggat
    10381 catatttaaa acttgaaaga tggaagtaaa aatattaaaa caagacggta gtgaaacatc
    10441 tcgtactgca attttaaatg acgagatttt taatattcaa cccaatgacc atgctatatg
    10501 gttagatgtt aaacgtatac aagctaataa acgcagaggc ctagctagca ctaaagaacg
    10561 taatcaagtg aaggggagta ggcgtaaatt atataggcaa aaaggtactg gacgcgcacg
    10621 taaaggcgat atcaaaagcc caatattacg cggcggtggg gtggtttttg gacctcatcc
    10681 tcgtgattat tctattaaag ttaataaaaa agtaaaagtc ttagctagaa aaagtgcctt
    10741 gtcttataaa gctcaaaata atcaaataat agtgatagat gatatgcaat tcgatgaggt
    10801 gaaaactaaa aaatttaaag aaattttagc taatttgaat attgaaaatc aaaaaatatt
    10861 atttgtattg gataaaccaa attattttgt atttttgtca gctaaaaatt tacaaaaagt
    10921 taaagttaca ttagcttcgc aattaaatac ttacgatatt ttaaatgcaa atttaattgt
    10981 atttaatgaa gcaagtttgc caattgttga ggagatatta ttaagatttc aaaaagttta
    11041 atcattatgg aacccaaaga tattataata aaaccacaaa taacagaaaa agctactttg
    11101 caaactgaaa aatttgctaa tagatatact tttatagtac atcagaaagc taataaatat
    11161 caaattaaaa aagcagtaga atggttgtac aaagtagaag tagatagagt aaatacgatg
    11221 aattttcaag gtaagaaaaa aacaagaggt acacgaacag gttggataga aggacgtaaa
    11281 ccttcttata aaaaagctgt tgtttttttg aaagaaggtt attcaataga tttttatagt
    11341 aatatttaaa agattgtaag taatgggatt aaaaaaattt aaaccagtaa caccaggatt
    11401 acgttttaaa gttataaatg atttctctga aatcactact gataaacctg aaaaaagttt
    11461 attagtacct attaaaaaaa ctggtggtcg taataatgaa ggtcaccgta ctataagata
    11521 tcgtggtggt ggtcataaaa gaatgtatcg agttattgat tttaaaagaa ataaagatgg
    11581 cattcctgct aaagtaagat ctatagagta tgatccaaat cgtagtgcta gaatagcttt
    11641 attagtatat gctgatggcg aaaaacgata tatcattgcc ccacaaggtc tcaaagtagg
    11701 acaaacagta atgtctggta gaggcattgc tcctgaaatt ggtaattgca tgtatctaag
    11761 tgaaatacct ttgggttcat tgatttataa tattgagatg cgtcccaatg aaggtggtaa
    11821 aattgctaga agtgctggtt cttatgctca actactttct cgtgataata aatatgcggt
    11881 gattaaattg ccaagtggtg agactagatt gataccagta aattgtaaag ctactattgg
    11941 tatgtgttca aatccagagc atagcttgat aagacatggt aaagctggtc gtagacgttg
    12001 gttgggacgc agacctagaa ctcgtggtgt agctatgaat cctgtagatc atcccatggg
    12061 tggtggtgaa ggtaaagcct ctggtgggca tccacgtagt cgtaatggta tatatgctaa
    12121 aggacaaaaa actcgcaaaa aaaacaaacc atcaaatcaa tttataatag aacgtagaaa
    12181 aaaataataa tgtaatatga gtcgctcatt aaaaaaagga ccttatgtcc actataaatt
    12241 ggaaaaaaaa gtattagaaa tgcaaagagc tcctagaaag caggttataa aaacttattc
    12301 tagagcttct atgattacac ctgattttgt aggacttact atcgctgtgc ataatggtaa
    12361 aacttttatt ccagtttata ttactgaaaa tatggtggga cataaattag gtgaatttgc
    12421 tcctactaga gtttttcgtg gtcatgcagg tcatagaaaa gaagataaaa gaaaataatt
    12481 attttaaatt attgctatgg gaaaaagaaa aagattaatg gcagaggaaa taaaaaataa
    12541 aaagagccaa acatattttg ctaagctcaa taaatgtcct atgtctgcta ggaaaatgcg
    12601 acctatcgca aatctcgtaa gaggtaaatc tattgaagat gccttagtta ttttaaaaac
    12661 tactcctaaa aaaggcgcta ggtttttata taagcttttg ctacataata ttgataatta
    12721 taagcaaaaa tctaaatcag gtcttagaat agaagaagct caattatata ttaaaacttt
    12781 aatggttaat caaggacctg cattgaagag atacagacct gcagctcgtg gaagtgctca
    12841 tcctataaga aaagaatctt gccatatata tatggaatta gcaagcaggg tagaggaaat
    12901 gagtgctaat attaataata gtgaagtcga atcattcaat aatacggagg tataatggga
    12961 caaaaaacaa atccaatttc taatagatta ggtattacta gaacttggga ttctatttgg
    13021 tatggaggta aaaatttttc tgaaaaacta atcgaagatc ttaaaattcg tcaatatttg
    13081 aattcaagac tcgaaaaagc tcaattatct cgtgtgataa tagaaagaac tattaagttt
    13141 ttgaatatta ctatttatgc tgcccgtccc ggaatgatta ttggtgcaaa aggtagtgaa
    13201 gctgataaac taaaagaaga acttaaaaga ttgactaata aagatgtaca aatatacata
    13261 catgaagtta aaaaacccga acttgatgct aacatagtag ctaataatat agcaaaacaa
    13321 atagaaggca gaatagctta tcgtagagct atcaaaactg ctattgctaa ttctattcgt
    13381 gctggtgccg acggtatcaa agtatcaata tctggacgtg tcggtggtgc tgaaatggct
    13441 agaaaagaag agtataaaga aggacaaata cctttacata catttagagc tgacatagat
    13501 tatgcattag ctgaagctca taccacttat ggttgcatag ggataaaggt atggatttgt
    13561 agaggtatca tatatcataa acctgatttt tatgggttag aatctgttaa tactaaaacc
    13621 agatcatata agcgtcctag acgttcaact catagaaaaa aataattatt cacaaattta
    13681 aaaataatta gaaatgttac aaccaaagaa aactaaatat aggaaagtac aaaaaggtag
    13741 aatgaaaggt aatgctaata gaggtactca attagcattt ggctcttttg gtttaaaaac
    13801 tctcgaacct gcttggatta ccgctcgtca aattgaagca gcacgtcaaa cagctactcg
    13861 ttatatgaaa cgtcaaggcc aactctggat acgaattttt ccagataaac ctgttactaa
    13921 aaagcctaat gaagtacgaa tgggaaaagg aaaaggtaat cctgaatatt ttgtcgctcc
    13981 agttttacca ggtagaattt tattcgaagt agatggcgtt ccactagata tagctaaaga
    14041 agctttaaga ttagccgctc agaagctacc tgtcaaaact aaatttatta ttagtaatga
    14101 ttatatagaa taacttaaaa aaaaaggaga aataaattat ggaaatatct gtaataaggg
    14161 aattatcaac taatgaatta aaagaaagat tagatgattt gaagcatgag tatgctattc
    14221 taaaaatgaa tcatgcagtt tctcctgtag ataatactgc taaatttaga gaaattcgta
    14281 aagatattgc tcgtattttg accgaattaa ctgaacgtga aaaaaaagaa aaaagtgaaa
    14341 atattgttaa atccgaatag taatggaaaa aattaataaa aaaaatagaa aagagcgaac
    14401 aggaatagtc gtgagtaata aaatggacaa atctgtcgtt gtacgtataa actatcacaa
    14461 aatgcatcca ctttataaaa aatatgttaa aaagtataag aagtttatgg ctcatgacga
    14521 aaataacgaa tgtaatattg gcgacaaagt acgaatagta gaaactcgcc ctctgagtaa
    14581 acataaacat tggagagttg tcgaaataat tgaaaggggt aaataattat gatacagcaa
    14641 gaaacacgct taaaagtagc agataatagc ggagccaaag aggttttgtg tatccgcgtg
    14701 ctaggtggca caggtaaaag gtatgctacc gtcggagata aaataattgt aacagttaaa
    14761 agtgctctcc cgtctggtaa tattaaaaaa ggaactgtaa ctaaagccgt tgttgtgaga
    14821 actaaaaaag aaattagaag agacgatggc tcatatatta gatttgatga caatgcttgt
    14881 gttttattgt ctcctacaga tgagctacgt gctactcgta tttttggtcc tgtacctcgc
    14941 gaacttagag aaaaacaata tatgaaaatc gtttctctag ctccagaggt aatttaagat
    15001 tatatgtgta aatttaataa atgattaatt atggctaaaa caagaaaaaa taaatctgaa
    15061 aaaccagtta agcttaaaat taaaaaaggt gattatgtgc gtgtaattgc tggtgatgat
    15121 agaggtgctg aaggtagagt attaaaagtt ttgcctaaag agcagcgtgt gatcgtagaa
    15181 ggtgttaaca ttattattaa acatacaaag ccttctgctg cccatccaca aggtggtaga
    15241 attgaagtag aagctccaat tcacatatct aatgtgatgc ttcttgtagg tgatactcct
    15301 actagaattg gtaaaaaaat caatgaaaat ggtaaattag ttagatatgc taaaaaaaca
    15361 ggggaggtga ttaaatgaca acaactactt atattcctag gttaaagaaa aaatattatg
    15421 atgatgtagt gcctgcaatg atgaaagagt ttgaatataa aaacattatg caggtaccta
    15481 aaattgaaaa aatagtcatc aatcaaggta taggggaagc tactcaagat cgtaaactta
    15541 tagatgctgc tatccaagag cttagcttga ttacgggcca aaaagctgta cctacgttcg
    15601 ctaaaagaga tattaataat tttaaactga gaaggggcaa cccaataggt gcaaaagtaa
    15661 ctctaagagg ggatatgatg tatgaatttt tagatagatt gatatctatc gctatacctc
    15721 gtattagaga ttttcatggt attagtgata atggttttga cggtaggggt aacttctctt
    15781 ttggtatttc agaacaaatt atttttcctg aaattgttat tgataaaatt aataaaataa
    15841 ttggaatgaa tattactatc gttactactg ctcgtaacga taaagaggca tactctttat
    15901 taaaaaatat aggcatacct tttaaaaaac attaatataa attactttaa aacattaaat
    15961 atggcaaaag aatcaataaa agcacgtgaa agaaaaagaa ggcaaatggt agaaaaatat
    16021 gccgataagc gcgcagaatt aaaagcaaaa ggtgattatg tggctttaca aaaattacca
    16081 cgaaatgcat caccagttag attgagaaat agatgtgcta tttctggcag acctagaggc
    16141 tatattagat attttggcat ttcgcgtatc gaatttcgtc aattagcctc taaaggttta
    16201 ataccaggtg tcaaaaaagc tagttggtaa taaaaaaaat agtatgttag taataagtaa
    16261 agcaagtttt ttcaataata gtatatataa agtatttaaa ttatattata aattactaac
    16321 aaaaaatttg aatttaatat ataatttgta attttgcatt taattttaaa aaaaaattaa
    16381 taaataaaga taaatatgat aacagatcca attgctgatt acctgacacg cattagaaat
    16441 gcgattagag caaagcatcg tattgtagaa atacctgcta gcaatatgct tatatctatc
    16501 actcatatac taaaagatga aggatatatc ttaaattata aagtagatga agaatctctt
    16561 cctcgcaaac tcattatagc attaaaatat cacccaacta tgaagattag tcctattcaa
    16621 aatttggtaa gagtgtcaaa acctggaagg cgtatttata aaaaagctga tgagcttcct
    16681 aaagttatga atggcttagg tattgctatt attagtacat cagaaggaat aatgacagat
    16741 aaagaagctc gtaagcgaca tattggtggc gaggttgttt gctatgtttc ataatcaaaa
    16801 ttaaggagtt agtaatgtct agaataggaa aaatgccaat aaaaataccc caaggagttt
    16861 ctgtagaagt ctccaaagat aatattgtaa gggttaaggg aaaattaggt caattagaac
    16921 aatatgttca accttctatt aaaataaata tcgatggaga tattatgact cttactcgaa
    16981 taaatgatga gaagcagact cgtgcatacc atggcttata tagatcgtta ataaacaata
    17041 tgataatagg agtttctgaa ggttttacta aaacattgga attagttggc gtagggtata
    17101 gggcgcaggc ccaaggacaa cttttagaat tatctttagg tttttctcat aatataatat
    17161 tcgaattgcc accagaaatc tcagttacta ctataactga gagaggctcc aatcctaaaa
    17221 ttatcttaaa atctttcgat aaacaactac tcggtcaagt agccgctaaa attcgttctt
    17281 tacgcaaacc agaaccttat aaaggaaaag gtattagata tgaaggtgaa tacgtacgtc
    17341 gtaaagctgg taaagcagca gcttctatta aataattaat taataatatt atggaaccta
    17401 aaataaagaa taaaaaagaa gcaagaagaa aacggataaa aatgcgcatc cgaaaaaaaa
    17461 taagaggtac tacagataaa cctcgtctat gtgtatttag aagtgataaa gaaatatatg
    17521 cacaaataat cgacgattcc caaaatatta ctattatggc tgtatcatca ctttgtaaag
    17581 aactaaaaga tctaaaaatg actaaaactg ataaagctag agaagtaggc aagttgatag
    17641 ctcaaaaagc tttagatgca gatatcaaga cggtaatttt tgatcgcaat ggttatttat
    17701 atcatggtag agtaaaagca ctcgccgaag gtgctagaga aaaagggctt aaattttaaa
    17761 ttttatggca aatatagtta gaaaagttaa agctgctgat gcagaattaa aagatagatt
    17821 agtatctata aaaagagtaa ccaaagtaac taaaggcggt aggacattta gcttcaccgc
    17881 gttagtagta gtaggtaatg aagatggaat agtggggtgt ggtttaggca aagctagaga
    17941 ggttccttta gctatacaaa aagccataga tgatgctaaa aaaaatttag taaaagttag
    18001 cattcgtaat ggcactatac ctcatgaaca aacaactaag tatggtgctg ctaaagttat
    18061 aatcaagcca gcagctccag gtactggtgt cattgctggt ggagctatgc gtgctgtttt
    18121 agaaagtgcc ggcgttaaaa acgtgctcgc taaatgtata ggatcatcta atccacataa
    18181 tgtagttaaa gctactataa aagctcttac agagcttaga gatccatata tgatagcaga
    18241 ccttagaaat attccacttg aaaaagtaat aggtaaataa aacttattta tgttatgaaa
    18301 cagataaaag taaccttagt tcgtagcttg ataggtcgtc ctaaagatca aaaaagaaca
    18361 gcagaagctt tgggactaag aaagttaaat catagtagaa tttttgatga caccccaagt
    18421 atacgtggta tgataaaaaa aatcgaacat ttagttaaag tagaagatat ttaaaattta
    18481 aaaattatgg atttaagtag tttaaaacca gcaaaaggct cagtaaaatc taaaactcga
    18541 gtaggtagag gtgaaggctc caaaaaaggt ggtactgcag ggcgtggtac taaagggcaa
    18601 aaatctcgta gtggatatag tcgtaaaata ggattcgaag gtggccaaat gcctttgcaa
    18661 cgtcgagtcc ctaagtttgg ttttaaaaat atttttaaag aaacctataa acctattaat
    18721 cttgatattt tacaagattt tgctgaaaaa caaaatatta ctatcattaa cccagatgta
    18781 ttatacgagt atggattgat tagtaaaaaa gataaagtaa aaatacttgc tcgtggcgag
    18841 ctaaaattga ctttagaagt atcagctcat gctttttcta aaaaagcaaa ggaagaaata
    18901 gaaaaattag gtggtaatgt aaaaattata aattgatgag taactttatt caaactttta
    18961 aaaatatttt taaaattgag gatcttaaaa aacgtatcct ttatacatta ttattagtat
    19021 ttatatatag atttggcaca catgtagttt taccaggtgt aaatgctagt ttgatcaaag
    19081 gcagtaccat tgcgcaacaa ggtcaaggtg gtttattagg catgatcaat atgtttgctg
    19141 gtggtgcgtt ttctaatgct tctattttag cattaggaat catgccatat attagtgcat
    19201 ctattataat acaattgtta actgtcgtta taccatcatt tcaacgtatg caaaaagaag
    19261 gtgaaagtgg tagacgtaga cttaatcaga taactcgata tttaactatt gttgttacag
    19321 tagctcaagc tcagggatat attacttatt tagctacgca attacctcgt caagctattt
    19381 atccttttac atctgctgga tccataccct ttatttttcg attttctact actattttac
    19441 taacagctgg aactttattt gttatgtggt tgggtgaaaa aataacagat aagggtatag
    19501 gcaatggtat ttctttaata attatggttg gtattgtagc tagattacct gtcgctttat
    19561 tatcagaatt tggttctcgt atgtctgtag caggtggagg tatatttgct cttattattg
    19621 aaattatact tttgataggt gtgattttat ttactatttt actcatacaa ggtactagaa
    19681 aaatacctgt gcaatatgct aaaagagtag taggtaatag acaatatgga ggagttagac
    19741 aatatttgcc tctaaaagta aacgctgcag gtgtaatgcc tataatattt gctcaagcta
    19801 tcatgtttct acctatcact atagctggtt ttgctaatta tgaaagtggt ggttggtttg
    19861 ttactgcttt ttctaatttt aatggttttt ggtacaatct tacatttgct ttacttatta
    19921 tagtttttac ttatttttat actgctatta cttttaatcc gcagcagata gctgatgaat
    19981 tgaaaaaaaa taatggtttt attccaggta ttaaaccagg taaaaaaaca gctgaatata
    20041 tagataccat aatgtctcgt attactttac cgggttctat ttttttagct attattgcga
    20101 ttttgccagc tattgtgagt aattttggta ttacatccca atttgctcaa ttttatggtg
    20161 gtacttcatt actgatattg gtcggtgtgg ttatggatac tctacaacaa attgaaagtc
    20221 atttgctcat gaggcattat gatggattta tgaaaaaagg aaaactaaaa ggtagatctg
    20281 tatacggagg tatttgagaa tgattatcag agatgtggaa gagctagaac taataaaaaa
    20341 aagtgctgat ttggttggta agacattagg tgaaatttct aaaatcctaa aaccaggcat
    20401 catagctagt gaaattgata aaattgcaga agaatttatt agagataatg gtggtattcc
    20461 tgcttttaaa aattataatg gctatccatc tactttatgt atttctatta atgatgtagt
    20521 agtgcatgga ataccaaaaa atcaagttat caaagaagga gatattgttt ctatagattg
    20581 tggtgcattt ctaaatggtt attgtggaga ttatgcttat acttttatga tagaaccagt
    20641 agatgaaaag attagaaaac ttgtaattac tacttatgaa gcactttata aaggaataga
    20701 acaagctgta gttggtaata gaattggtga tataggattt gctattcagt tatatgtaga
    20761 gcgtaacggc ttttcagttg taagagaatt agtaggacat ggtattggca aaactatgca
    20821 cgaatatcca caaataccta attatggtaa aaaaggtaaa ggttatttat tagaagatgg
    20881 aatggtaata tgcatagaac ctatgataaa tatgggtaaa aaagaagtta tttttggtaa
    20941 tgatggttgg acagtgctga ctaaagatca caaaccttct gcgcatttcg aacatatgat
    21001 ttggataaat caaaaaaaac ctgtaatttt gtcaacttat aattatattg atgaaaaatg
    21061 gaaggttaaa tagttatgcc aaaacaaaat gttattgaaa aagatggaat agtaagggaa
    21121 agcttaggaa atgctatgtt tagagtagaa ctagataatg gccatgtgat attagcacat
    21181 attagtggca aaatgcgtat gcattatatt cgtattttgc caggagacaa agtacgtgtg
    21241 gagatgtcac catatgacct tacaagagga agaattgttt atagatatta aaaaaaaatt
    21301 gaaaattatg aaagtaagag cttctgtaaa aaaacgaaca cctgatgata aaatagtaag
    21361 aagaaaaggt agggtatata ttatcaataa aaaaaatcct aaatataaac agagacaagg
    21421 gtagtaaata ttaaaaatta aaaaaaatat ggcaagaata gttggagtag acttacctaa
    21481 aaataaacat ggcgaaatag cacttaccta tatttatggt attggtagga gtactgctcg
    21541 ctatatttta agcaaggctg acgtagactt tgataaaaaa gtaaaagact ggacagatga
    21601 tgaaataagt aagattcgtc agattataaa tgatgaaatt aagacagaag gagctttacg
    21661 atcagaagtc caaatgaaca ttaaacgatt agtggatatc aattgctatc gagggatgcg
    21721 tcaccgctta ggattacctg tacgtggaca gcgtacacgt acaaatgcac gtacccgcaa
    21781 aggtagaaga aagaccgtag caggtaagaa aaaagcaccc agaacataat aattaaaaaa
    21841 aaatgaatta attatggcta aaacacgctc aagaacagct agtaagaaaa gagttgtaaa
    21901 agttgaacct tttggatgtg cttatatccg agcaactttt aacaacgtta tagttacctt
    21961 aacaaacgaa aaaggagatg taatatcatg gtcatctgcc ggtaaaatgg gttttagagg
    22021 tagcaaaaaa aataccccct atgcagccca agtagctgca gaagatgctg ctaaaacagc
    22081 attagatgcg ggattaaaaa aggtcaaggt gtatgttaaa ggaccaggtg cggggcgaga
    22141 aagtgctatt cgtaccatcc ataatgcagg tatagaagtt actactatta ttgatattac
    22201 tcctgtacct cataatggtt gtagacctcc taaacgacgt aaagtataaa ttttaaaaaa
    22261 aaattaaaaa atatggcaag atatataggt ccaaaaacta aagttgcacg ttcattgggt
    22321 tcaccagtgt atggatatga taagtattat gaaagaaaaa attatcctcc aggaatgcat
    22381 ggtctatcaa gaagaagaag aaaacctagt gaatatagaa tgcagctatt agaaaaacaa
    22441 aaagcaaaat atacttatgg gcttaaagaa gcacaattta gaagatttta ttctatagct
    22501 ttatctaaag gcggaattac aggtgaaaat ttaatgcaat tattagaatc tcgcttagat
    22561 aatgtcgttt atagattagg tatagcaaaa acacgacctg ctgctagaca attagtttca
    22621 cataaacata tcatagtgaa tgacagagta gtaaatattc catcttatct agtaaaacca
    22681 ggtgacatta taggtgtgag agagagatct aagtcattag aaatcattca agaagctata
    22741 actggcagag tagataaata ttcctggttg caatgggacc aatctaatat ggcaggtact
    22801 tttttgcaat atccagttag gacagaaata cctgaaaata ttaatgaaca attgatagtc
    22861 gaattatata gtaagtaaaa tattatggca atattggatt tccaaaaacc ggatcaagtt
    22921 attatctcag aaaatactga gacttatggt gtttatgaac ttcaaccatt agaacctgga
    22981 tacggcatta ctatcggtaa tgctcttcgt agagtgcttt tatcttcact agaaggttat
    23041 gcaattactc aatttagcat taataatatg ccgcatgaat atacttctat tgatggagta
    23101 gtagaagaca tcatatatat tggtctaaat ttaaagcaag tgagattaaa accaaatttt
    23161 gataatgtag aaaatgaaag ttttagtata attattagcg ggcaagatga atttaaagct
    23221 ggtgatttga ataagatttt aactagtttt actgttatta atccagattt acatatatgt
    23281 actatggatc catcagtaga gcttaatata gaatttaaaa ttcaaaaagg acgtggctat
    23341 gtcttagctg aagaactcaa agatcccgag gcccctatag gtactattac ccttgatgca
    23401 gtttttacac ctattaaaaa tgtagcttat tatattgaaa attttagagt aggacaaaaa
    23461 actgattatg aaaaattaat tttagagatt aatactgatg gtactgtaag tccatatatg
    23521 gctttaaatg aagctgctaa tatactttta cagcatatag aaattttctg tgaaaaaaaa
    23581 tattcattgc aaaatgattt agaccaacta gatgatgcaa aagatgatga tgtagatgaa
    23641 aattatgaac aaatgagaaa acttttgcaa actaaacttt ctgatttaga tctaagtgtt
    23701 agggcaatta attgtttaaa agctgcagat attgaaacat taggacagtt agtcagttat
    23761 agtaaaaatg atttacttaa atttagaaat tttggtaaaa aatccttatc agaagtagaa
    23821 gaagttgtaa aatctaaaaa cttatatttt aatatggata tttctaaata taatttagat
    23881 aaagaataaa attatgagac atagaaataa aattaatcat ttagggcgga ctcatacgca
    23941 tcataaagca ttaatgagaa atttatcaat tgctttgata aaacataaaa gaataaatac
    24001 tactgtgcca aaggcaaaag aattacgaca atttatagaa cctattatca ctcttactaa
    24061 aaatgatact actgctaata gaagattagc tttttctaaa ttgcaaaata aagaagctgt
    24121 aaaaatttta tttgacgaaa ttgctgaaat ggttgctgat agaccaggcg ggtacactag
    24181 gatcttaaaa accggatatc gtgttggaga cggtgctcat atgtgtataa tagaacttgt
    24241 agacttcaat cctgaatatt ctcaagctag gagcactaaa gcagctacta aacctaagac
    24301 aagacggggt ggtggtagag gtaaaaaagt ttctcaacag ccagatataa aagaagatac
    24361 taaagctgag accaaaccaa tcactgaatc agaaatagaa tttgataaag aagatgcaaa
    24421 agctgaagca gatgtaaaag aagatactaa agctaaaacc aaaccagtca aagaatcaga
    24481 aatagaatct gataaagaag atgcaaaagc tgaaccagat ataaaagaag atactaaagc
    24541 taaaaccaaa ccagtcaaag aatcagaaat agaatcnnnn nnnnnnnnnn nnnnnnnnnn
    24601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24661 nnnnnnnnnn nnnnnntgat aaagaagatg caaaagctga accagatata aaagaagata
    24721 ctaaagctga tgataataaa tctgataatt aaaataatca agattaataa gcttatataa
    24781 atctcatcat tctatcttaa tgacaattta attgttatta attttgcaag gtacttaata
    24841 taatatattt gtactaattt aaaaaattgt tttattgatg tttttttact atatatgtgg
    24901 tttgtaaata cacttaataa atctttttat ttttattaat ttctaataaa aatgctatta
    24961 tctgttgtcg gttatcctat agcacatagt ctaagcccca tattgtttga gtatttatct
    25021 tataaatatc atatatctat tcattattct agattagcaa ttgataatct agatgatttt
    25081 aataaaattt ttaatgaaaa tctagatgtt tttgatgggt ttaatattac ttatccttta
    25141 aaaaaagatg tatatcatat gtgtcagcat tattctaata tacctacagg aattcattgt
    25201 tgtaatacta tagtttgtaa acccagcata tcaatttata atacagatta tcatgcaatc
    25261 aaatatattc ttagtagaga agttaaaaat ctgtgggaac aagttatcat actaggctct
    25321 ggtatgactg cttctatctg tgcttgggca ttaatcaatt attataaacc aattattatt
    25381 ttttatcata aaaacattaa ttatgaatat tttcctaaaa ataaaaatgt tcattggaaa
    25441 caactaaaca aaattaatga gagtataaat gataaaacgt tgttaatcaa tactttatct
    25501 ccaatggcta attatgaatt tttaccaaaa aaattaaatg cagttactac tatcattgat
    25561 actgattata gaaatcttcc attaaaaaat gttgctatta gtaacaatat caactatatc
    25621 gatggttatc actggcttta ttatcaaggt atagaagctt tcaaaatttt tactaataaa
    25681 acaaatttgt ctgtagaata taatgaagaa tggataaaaa taaataaata tgataaaata
    25741 gcattaatag gtatgagtgg agcaggtaaa actactattg gacagaaatt agcaaagaaa
    25801 ttaaactatc aatttgtaga tattgacgag ataatagaaa agcaagagca taaaactatt
    25861 tcagaaatat tcgctgatga tggagaagat ttttttcgtc aattagaaac tcaaatgctt
    25921 gctaaaataa tcaaaaatga gaaaataata atagctacgg gtggaggcat catcgagtct
    25981 gaaaaaaata gaaaattatt gaaaagcaat ttttttaata ttttattata tgtaaatgta
    26041 gatgaagcta ttaatagact aaataatagt aaaactagac cactgctaac tggcaatatc
    26101 aaaggtaatt gggaaaaatt atggaatgac aggcgcttat tatattatga aacagcagac
    26161 tatatcatta atttggctaa aaataatgaa gatttaatat atgaagatat caaggaaatt
    26221 attaaaaaat aacttatcaa tatctctgct aggatctaaa agcattgtca tacgccatct
    26281 tttgatttca ctatttcata atagtaaaat aatattaaga aatgtacaat tttgtgatga
    26341 cgtagtagta gccattaatt taataaaaac ttatggtaaa atagtagata tagtcgataa
    26401 aaatacaatt aaaatttctg gacaaataac aaaaatacca gaaaaaatat actgtggaga
    26461 gtcagctagt ttgctaagaa tgatagtgcc gatacatcat agctttcaca ataaatgtag
    26521 atatgatggc gacaaaacat tgctagtgcg cgatttcata gatatagaag aaatgttaag
    26581 ttcacttgga ttgagtttta gctctaccaa attgccattt atagtaatag gagaattgaa
    26641 ttcacaaaat aattattctt tgatggcaaa gcaaagttct caagtagtat caggattatt
    26701 aatttcgact tgttttcaaa atttagttac tacaattcat tgtcccgagt tagtaagtgc
    26761 tccttatgtt tatttaactg ctgaaattat aaatctatat ggtggtatga taacatacaa
    26821 tgataaaaca tttcaagtaa aaccaaataa tttacaagag aaatctaatg attttttaat
    26881 cgaaggagat tggtctttag gtgcaatgat attggcttta gggttgactt ttgaaagttg
    26941 cacggtaaaa aatttatcat caaattcgct acaaccagac agtgagattt tatcgctttt
    27001 tgataattta tcaataaaat atcaagttac agaaaatagt atcattactt ataaacaaaa
    27061 ttataatgga tttagttatg acattactaa tacgccagat ttagttccag ctctaatgat
    27121 tttatccgca aatgccaata gtccatctgt gataactggt attaaacgtt tgactaataa
    27181 agaaagtaac agagcaaata cattggctga aaactttata aggcttggtg gtagattgga
    27241 aacaacaaat gaaaatactt ggataattta tccctcacaa atacatggcg gggagattaa
    27301 aacttatgag gatcacagag tagccatgct cggaatattt cttggtttat tatcagaaga
    27361 ggatgtagaa atagataatt ggcaatgtat tagcaaatca tattggataa agtagcttta
    27421 gtataaaaaa attcgattaa tataaaacat atgacctgta acaaacaaca aatattttgt
    27481 aactaaaaat gaatatactt caaatattta agttattaat tataaataca caaaaaaaga
    27541 aaattaatag atcagtgagc ttataagata attataatat ctcaagtggt attattttgc
    27601 aaattaacta taataaatat ttttggttca atatttgcat atttttaatt aaaaaaaata
    27661 aaaatgaaac ataatttatt atttatcatt atttttttat ctacattatt tatcaataaa
    27721 tcatttgcaa gtgacaatac aagattattt cctggagatc ctatcataga taaaataata
    27781 gcatgcctaa aaactcaaga tgctcgttgt gtaggtcaat attttgctaa taatattgaa
    27841 atgaccttat tgagctcaaa aggtaattat tcgcgtagtc aggctgtggc cctattagat
    27901 aatttttttg taaaacatcc tctgaaatct atctcaaatg taaaatctgg agagttgtct
    27961 gataataaaa tatatgtagt tggcaattat aatagtggat ctataacgta taaaatttat
    28021 tttatattat ttaaagaaat aaatcaaatt gcaccacaaa tattaatttt taatataaca
    28081 aaataaaatt atataaaata atgcttaatt ttgtaaaaaa attatggaag agaatgtacg
    28141 accacaaaat aatgaaaatg tagaaaatca agataaaaat gtaaatgaac aaccaaagaa
    28201 aaaatcttgg tttaagcgca atgtaggtgc taccattatt atgtctttga tgcttattgt
    28261 catcatttat cttggcatta atttgatgtt aaaacaaaaa catcacagag aagagctaga
    28321 aaatcaaaga gcacaatata cttctcagat cgatagctta aaaaaagaaa attatacgtg
    28381 gctaacaaaa gttttttcat ggagcatacg aagtgaatgg atgagaaata atttagaaca
    28441 agtgttatgg tatagtgaag atctactaaa atttggtgat attaataaca taaaacttgt
    28501 agatcctagt ggcagagtaa tattttctac caatatgaaa gatcaaaacg ttatcaccga
    28561 atggtctaat attcagaaaa tcgaaataca agaaaaaagt gataaactag tagtagcttc
    28621 acctataatg ggaacagata tgccaatggg tgctgtagta atagaatata aaatcaataa
    28681 acaaattaac gatactgaaa attaaacaaa taataaattt ctaaaatttt taatctaaaa
    28741 actgcgatta gtagcgttat agaatgtatt tgttcgaaaa tatagatata gtagtaatag
    28801 gtgcgggaca cgctggttgt gaggcggcag tagtgtcggc tagattagga tctaaagtac
    28861 tattaataac tttagatatt gctaatcctg cacgtttatc atgcaatcca agcataggtg
    28921 gcatagcaaa ggggcaaata gtaaaagaaa tagatgctct aggtggatgt actggtatag
    28981 tagcagatag aagtatgata caatttcgca tgctaaatag gtccaaagga cctgcaatgt
    29041 ggagtcctag atcacaaaat gatagatggt tattttcgta tttttggaga gaaatgctag
    29101 aaaatacacc aaatctatat ttgtggaaag atgaagctat agatatatta cctaaaaata
    29161 gtcatatagt cgtaaaaact aaaatgggaa tagaaataaa ggctaaagca gtaatagtaa
    29221 cagcaggtac ctttttaaat ggtaaaatat atataggtcc cttacaaata gctggcggaa
    29281 gacttggaga acctgcagct gtaggattga cagaatcatt aaaaaaatta aacatcgaaa
    29341 gtggtaggct caaaacaggc acacctgtta gattagatgg acgtactatt gatttttcaa
    29401 aaatgataga gcaaccagga gatgagaaac catctaaatt ttcttattca gatgatacta
    29461 aaccattaga aaaacaaaga agttgctgga tcacctatac taatgaaaag acacatgata
    29521 ttttgcaaaa aggatttcat ttatcaccgt tatttaccgg tataatcaaa ggagtcggtc
    29581 ctcgatattg cccgtcaata gaggataaaa tagtaagatt cagtaataaa aattctcatc
    29641 aattatttgt tgaacctgag ggctggaata cctatgaata ttatttaagt ggattctcat
    29701 cttcattgcc attagacatc caatatgagg ctctacatac aataaaaggt ttagaaaatg
    29761 cccatattat tcaacctggt tatgcaatag aatatgatta ttttccacca aatcagttaa
    29821 aaaatacatt agaatctaga atagtagaaa atttatattt cgcaggacaa gtaaatggta
    29881 ctactggcta cgaagaggct gctgctcagg gaattatcgc aggagtaaat gcacatcaaa
    29941 aaatcaataa taaacaacca ttaatattat cacgtagtca agcatatatt ggtgttttga
    30001 tagatgacct tatcactaaa acacttgatg aaccgtaccg catgtttact agtagagcag
    30061 aacatagatt gatcctacgt caagataatg ctgaccttag gctttcacct atagcttacg
    30121 aacttggatt aattgataaa gaaagataca aaaaagtaga acaaaaagaa caattcataa
    30181 atcaaactat taaatatctt agctctaagt cagtagatcc tgaaaaaata aatgattatt
    30241 tgatttcaat aaattcatca ccattaaaac aaaagattaa gtggacgcag ttattaatga
    30301 gaccagaaat ctcattacaa gacttagcta ataatataga tgaaataaat aatttaataa
    30361 aagataagcc tttgcatttc atagaaacat tagaaataga aatcaaatat gccgaataca
    30421 taaataagga aaatgaactt gctaaaaagc tcatgaaatt tgatgagatg catctaccta
    30481 ccgattttaa ttatcaagaa ttaaaaaata tttctattga ggcacgtcaa aaattaaata
    30541 atgtaaagcc agcaaatata ggtatggcta gtcgtatcac tggaatatct ccagcagata
    30601 tagcattgat aatcaattat ttttataaaa aaaaatctaa ttttaattaa cgatgttaga
    30661 ttgataatag ggaaatttct aaattactca tcttttccat ttcgcattct cacagtcttt
    30721 gtctgcttgc cgttcttatt tgtgttttaa tattagtata tattagcgta tctaattgat
    30781 gtaattataa ttcttttatt aatttttata ttattacttg tagatatgat ttatatttat
    30841 ttttatttat ctaataaatc aatagcaaga tatcttgttt tattattgaa atctttttca
    30901 aaaaagtaag atatttgagt gtaattactc aaaaaatcag taataacatt gtagcaaatc
    30961 ggatttattt cgaaaaaagc aaaccttgca tctggacttt tgaaaaaatc attgataatt
    31021 tttttataaa aaattatagg atcttcatcg aaaacaaata aagcatcatg aggctcccaa
    31081 ttttttacat taggttttat ttcatttttt tcaatattta aaacataagg tggattacta
    31141 atgattacat caaatttaaa tttagataaa tcagtattta atatatctaa ttgaaaaaaa
    31201 tcaattgtta agttatattt tatagcatta aattttgcta atttaatagc ttcactcgat
    31261 atatctatac ctattactct actataagga taataatatt tcatagctaa tgctatacat
    31321 ccggtgccag taccgatatc cacaatagtt ttaggtttat atttttgttt ttcgagatta
    31381 tttatgacta atttaactaa ttcttcagtt tctggcctgg gaattagtac attttcattt
    31441 atttttaatt caaaatttag aaattgtgcc ttaccaagta tatattgtaa tggcttttca
    31501 ttttgtaact gctttattat ttcaaatagt ttattaattt catcattatt tagttcatat
    31561 ttgggtggta ataataaata atcaatagtg tttatattta atatatgcgt tattatatct
    31621 ctaattaatg gttctacgga aagatgataa aaatattcgg gtatagcttt ttttaatatt
    31681 ttttttgctt gatataaatt cattttttaa ataaaattaa taaaaacaaa ttatatttga
    31741 ttaatgaata tattatgatt gagttttata atgcttgctt aaaataattt ttgtagattt
    31801 ctgaataata ggaatagtat taaaattttt ggagttgagg agggattgag aaattaatat
    31861 atgtttcaat taacatatgt tatattcact aactctctat accgatacca cgcccatctg
    31921 tc
//
LOCUS       DAOPRN010000019        31831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1100_length_31831_cov_137.537072, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000019 DAOPRN010000000
VERSION     DAOPRN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31831
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1100_length_31831_cov_137.537072"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..2404
                     /locus_tag="PKI65_03630"
     CDS             179..2404
                     /locus_tag="PKI65_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOB77567.1"
                     /translation="MNNLFGLSEEESKYLVNRYRYLLHTLRYNTSKENRALIRKAFNL
                     ASEAHKGMRRYSGDPYIYHPLEVAIICSEEIGLGTTSIVSALLHDVVEDTDYTLDDIR
                     NHFGDKIASIVDGLTKITDFIELSKSDDTSIQAENYRKILITMADDVRVILIKLADRL
                     HNMRTLNSLPPHKQQRIAAETSVIFAPLAHRLGLYNIKSELEDLALKYNDPEIYNYIK
                     YKIEETSSERDAFIKDFIKPIKERLDQTNLKYKITWRTKAISSIYRKMTEKQLNFEEI
                     YDVFAIRIILDSPPENEKSDCWLVYSIITDIYTMNPERLRDWISVPKANGYESLHVTL
                     MSKSGKWVEVQIRSKRMDEIAEKGYAAHWKYKENNGKISTLDEWLNSIRNILENPNEN
                     ALDFLDDVKFALFTDEIPVFTPKGKLLQLPKGSTVLDFAYAIHTDLGDHCISAKVNHK
                     TVPVNTVLKSGDQVNILTSEKSSPNPEWLNFVKTSKARNAIKAALRKQELALAEHGKE
                     IIERMFTQLKEKYNDNLLQKFANYLGYNNIKQLYIAAAKGQFDVKQLKQFLQSQKPST
                     FSRLFRHYSNSNKNSYQSNEIEEKEKIQTKIILDTTQQINYILSECCSPIMGDQLIGY
                     ISDPHTLEIHRTTCPVFKEKAAVHGDRVTKVKWEENRPLEFKTSIELEGSDRKGIVKD
                     ISKLISEEMNSNIRYFFLEVTEGTFKGEIKLYVNNIDHLKQLIDKIKTIDGVSKVYRK
                     E"
     gene            complement(2541..3803)
                     /gene="hutI"
                     /locus_tag="PKI65_03635"
     CDS             complement(2541..3803)
                     /gene="hutI"
                     /locus_tag="PKI65_03635"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOB77568.1"
                     /translation="MNNKKLFVNIGSLMGISENYPNRPLKGAEMSRIDKIENAYLSVE
                     GDTIIDYGMMSELGELSAIYKNYDNIYDVNKRIIIPGFIDTHTHIVFASWREDEFIMK
                     IQGIPYEEIAKKGGGILNSAKKVQEKSENELFDLALKRINEVISYGTVALEIKSGYGL
                     SLESELKMLKVIKKLKEYTNLPIKSTFLGAHAFPIEYKDDREGYIDLLISEMLPKVVD
                     EGLADYVDVFCDEGFFTIDETDKILNAANKFGLKPKIHVSEMSNNGGVQLGIAHNALS
                     VDHLERITDEEIRALLLSKTLPTVLPQVSFFLKIPYAPARKMVDAGLPIVIASDYNPG
                     TSPSGNLQFAISLATIYQKLMPEETINAVTYNAAFALELEKQLGSIEKGKKASFLILN
                     SDVTNLNFFPYHFASNKVDKVIINGEIG"
     gene            complement(3858..11060)
                     /gene="sprA"
                     /locus_tag="PKI65_03640"
     CDS             complement(3858..11060)
                     /gene="sprA"
                     /locus_tag="PKI65_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOB77569.1"
                     /translation="MKKISTHSIPFIRCIFVLSIFSFFIFYSFSPKYYYVSNELNIQH
                     FPGPPDTLDSLKYPIGNQQIIGPPPLIISPWDVNPKNLKDTIIYDPSTNTYEYQQHIG
                     NIKLRYPYEMTFDEYSEYDFQKAWQREWQKQITSQSKGKSLFDSLFRMHVKDEGVFGS
                     GLIDIRSSGTAEIIFGYKGYRRDDPSLTVKQRKYGNLDFQEKIQLNLMAKVGDKVNFN
                     VRYNTESSFDFDNKIKLEYEGKEDEIIKKIEAGDVTLPLNSTLISGSQSLFGLKTQLQ
                     FGKATVTAVLSQQESQTSSITVAGGAEVREFKIEALDYEENKHFFISQYFYNNYNKFL
                     ERLPIISSPVNITKIEVWITNIGPATQENRNIVAFQDLGEYQPYNQDILPNFTPDAYL
                     PSNRSNNLLQIVNPANIRNLNDVNSYLSAQNLKMGTDYEKIENARKLSPSEYSVNNQL
                     GFISLNISLRPDQVLAVAYQYTVIGSDSVYQVGEFSTDGIDPPNCLVVKLLKPSSINT
                     KLPIWNLMMKNVYSIGGYQISNEDFRLNIIHKNENLGVGAGYLTESNLKFIPLIRVLG
                     FDKLNINGDPEPDGLFDFLDNAATSGGTINSRNGRIYFPVIEPFGKDLRTKFDDPAMA
                     DKYCFDSLYTTTKTVAMQIANKNRFTIEGSYKSSVGNEINLNAINIPQGSVKVTAGGI
                     LLQENVDYTVDYTMGRVKIINQGILSSGVPINVTVENNTFFNFQTKTYLGAHVDYKIN
                     NNFIVGGTIIHLSEKPFTQKVNIGYEPISNTIWGANISYQTESRLITNIIDKLPFVST
                     KTPSSIQIDAEVANLIPGHNRAIGKEGNAFIDNFEGSMTSITLKNPFAWHLASIPQGQ
                     SNIFPEASDDTTLTSGYNRALLSWYTIDPSVFFRNNTTTPPNVNKEMLSNHYMREILE
                     SELFPAKEFPNGIVPNLATLDLAYYPSERGPYNYDIVPTNVSAGIDANGRLADPETRW
                     GGIMRTIETPNFEETNVEYIEFWLLDPFIYNPASPGGKLYINLGDISEDLLLDHKKAF
                     EHGLPTGPEMIGIDTTQWGVVPKYQALVNSFDANLDARKYQDVGYDGLSDENERSFHS
                     SYLQAIEAIYGASSIAYQLAYQDPSADNYHYFRGGDYDNLGYNILQRYKYYRHPEANS
                     PTPEMSPESYPTSATNNPNSEDINRDNTLNELERYYQYEINLRPDQMEIGKNYITDIY
                     IASVKLPNGNSEQVKWYQFKVPVKRPDKIVGDIRDFTSIRFMRMFLREWNDSVVLRFG
                     SLDLARSQWRRYSYSLLQPGEYIPENEQNLTTFDITNLNIEENGTRKPIPYVMPPGIE
                     REINYGTTNLQKLNEQALSMKVCHLIDGDARGIFKSTDYDFRYYKKIKMNVHTEEVDP
                     LNYSLKDGDLTLFIRLGTDFDRNYYEFEIPLHPTPWGNYGHDNESRKRIWADSIDLSL
                     DKLVELKLERNTILRGEDNTNLTLFTPYSKEDEKGKYTVVGNPNLAEVKVILIGVRNP
                     KAGTYTDDDGMPKCAEIWVNELRVNTFDEKGGWAATARMNIGLADIGNIMFATTLSTA
                     GFGSIEKKVYERQLDNISSYDLALNIDLGRLLPENFYLRIPMHIDYSEMIANPKYNPL
                     DPDVYLKDDLSTYATDREVDSVKRLVQSYTQRKGINFMNVKKLRPPTAKGNPKFYDLS
                     NFDISFAFNQIYIRNPDIEYNKKKEYSGTIAYNFNGNPKNYMPFKNVKFLNNKYLKLI
                     GNFNFYLLPKSISLSTDMYRLYEENRWRNKTTNLVIIEPNFIKNFMWNRNYAFKYDLT
                     RNLRFDYTAVANARIDEPPGRIDKRESDYKEKRDSILRNIVNFGRITNFNQRIQGNYT
                     LPLNYIPILDWMNASAQYTGNYTWMAAPLYKDSTDQYVEHPYGNSIQNSRTISFNVNG
                     NFTRLYTKVTYLKNILNPPPPPRLQDEEDTIKQGPTIGKQILDNFLRLLMMTKNVSFN
                     ISFSDATMLTGYRGVPQALGMEFNNMTPGWGFVFGWQEDVRDRLVAKDLFTKDTTLNT
                     PYLTNHTENMNIRVTLEPIKYLTINLTAIRNKSMNNREYFRYNGSEFQSYTPLTTGSF
                     SMSIISSKTAFAKNAKDFSNKNFQILLDIRPEIAQMVAERSPVWDGTYVADSVTGILY
                     PDGYGATSQNVLIPAFLAAYTGHKPSKYVKSIFPSFPMPNWRISYTGLSKIEFLKKYF
                     NSATLTHGYTSNYTLSSFSNNPLFSDPDGDGYTTVRDALHNYLPQYEIAQVSISETFN
                     PMFGIQLNWKNSLVTTIDWKKSRNIALNTSNVQITEINTNEWVIGLGYRFKDLAFDIR
                     TGGQTKTINSDLNLKADLSIRDNITVLRRIVEEINQVSTGQKIISINLSADYQITNNI
                     TIRAFFDKIINKPHVASQYYNTSTNFGISIRFSLAM"
     gene            complement(11073..11663)
                     /gene="ruvA"
                     /locus_tag="PKI65_03645"
     CDS             complement(11073..11663)
                     /gene="ruvA"
                     /locus_tag="PKI65_03645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOB77570.1"
                     /translation="MLNYIKGLLVSINPSKVIIESLGMGFEVQITINTFEKIKESKEI
                     TVLTHLILKDDGIQLYGFADEDEREAFRLLITVNGIGPNTARNLLSTLTPIDLYSAIL
                     SKDVTSLQKVKGIGIKSAERIIIELKNKIPEIQQSLKNLSNFDTNFYQDALLALKQLG
                     YSVKEAENALNKVLKNHSKEEYVSLDKLIKLAFKYL"
     gene            11819..13717
                     /gene="dxs"
                     /locus_tag="PKI65_03650"
     CDS             11819..13717
                     /gene="dxs"
                     /locus_tag="PKI65_03650"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOB77571.1"
                     /translation="MPKYPLLAKINGPEDLKKFSLKELKSIANEIREYIIEVMCNTPG
                     HVGANLGVVELTIALHYVFNSPDDKIVWDVGHQCYPHKIITGRREEFRTIRKFKGISG
                     FPTREESIHDVFGTGHASTSISAALGMASAEKIINPDSKNNFIAVIGDGSLTGGMAYE
                     AINHAGTTNTNFLIIYNDNNISIDESTGALKNYFTHFIASKSYNQLKNTAYEHLKGTL
                     IRRFFNKISSIIKNTLLKHSNFFEFFNLRYFGPVNGHDIEQLVKILNNLKNIPGPKIL
                     HVLTQKGRGYPPAEKNAVAFHSPGKFDPETGEIFSNRNDKNSPLRYQDVFGNTILELA
                     DMDDRVVAVTPAMVSSSSLDIMYAKYPNRVFDVGIAEEHAVTFSAGLAAQGLIPFATI
                     YSTFLQRGFDQIIHDVALQNLPVIFAIDRGGIVGEDGKTHQGVFDLAYLNLIPNMIIS
                     APIDCIELRNLMYTAYKYRMGPFAIRYPKAIAEFVNFDKPFELLPIGKAQILREGEKV
                     LIISLGNPGHDVVELYPEFDNLEINPTHVNLRFLKPFDVDTLNQLCKNHEIIITIEDG
                     TLYGLSSTISDFLVKNKFNNKLISLGVPDQFIEHGSINELKQMLKYDKNSILNTVIQA
                     YKGIYKLS"
     gene            13771..14871
                     /locus_tag="PKI65_03655"
     CDS             13771..14871
                     /locus_tag="PKI65_03655"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOB77572.1"
                     /translation="MKKAVHIVRILIGLLFVFSGFVKAVDPWGFQFKLEDYFIAFNWD
                     FFMPFALFLAILVPAAEFIIGFALLLNLKPKYAIWGALIFMAFFLPLTLYLAITDAVS
                     DCGCFGEAIKLSNWNTFYKNIIITAAIIFVIWQRKYLKSWLKNAVEWILIAVSGLFII
                     GVSVYGLTYLPIVDFLPYKIGVDLKPDPNLKDKYFVIYKNKTTGQLEEYPANQYPWDD
                     SLWMAEHEFVDTRVEKAAKSALLILTYDNDGNDVTDSVLLSKKPTMLIVSYDIKKVNP
                     KTATKLKEWINIANKFNVNTFLITSSDENLVKKQLKNSQIDIPYVFADDIALKMLIRA
                     NPGIVMMRDGVIKAKYTLNHKANEKKFDKLLR"
     gene            complement(15020..15787)
                     /locus_tag="PKI65_03660"
     CDS             complement(15020..15787)
                     /locus_tag="PKI65_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOB77573.1"
                     /translation="MKTNIKTLLDTYYQEFSQPKYWQVDPIIVPAIFSDPLDIEIAGF
                     FSAIIAWGKRENIVKAAKNLMQLMNYQPYKFITGNKKNWNSCESFVYRTFQPMDLFYF
                     LDALRLIYFKYDSLNNFVKINYDKNRDLIDSIRAIRDIFLFVPHEQRSEKHIPNPDNN
                     SACKRINLYFRWMVRTNKEGLDFGIWQNIPSSSLYIPLDLHVGMAARELGLLSRKQND
                     LKAVIELTYNLKQLDPFDPIKYDLALFGYNYYKNDVL"
     gene            complement(15861..16901)
                     /locus_tag="PKI65_03665"
     CDS             complement(15861..16901)
                     /locus_tag="PKI65_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HOB77574.1"
                     /translation="MRLFTINNEGKLIPFKEHTFKEENNEFDLENLLEKNPEYFFEDG
                     EIMIIGRQVQTNLGSLIDLLGLDKNGNTIIIELKRDKTPRETLAQLLEYASFVENLDY
                     ELLNEIFQEYVGEEISLDNYHQEYFKITGDANVSFNKNTKLVIVAQDITNNIKQTALY
                     LRKKGVDIYCIEFKWFQNNALEKMITCDFIIGEESYIKGEVKSKTQLPKVNEITFLES
                     VDENGKEVFERIFKFAESNGLSCRWGSKGFSLNLPIESSFVGLCLGYPPESVFKQSIY
                     SVIEQIDKKVNDAETIIDYFKKELIKTQFFEPAGSNYKWIIDKPVDNTTISTFLDILT
                     EVISQIKQKGLK"
     gene            complement(17209..17754)
                     /locus_tag="PKI65_03670"
     CDS             complement(17209..17754)
                     /locus_tag="PKI65_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOB77575.1"
                     /translation="MTEEIIIAGFGGQGVQSMGKILATSGMKQGLNVSWFPSYGPEMR
                     GGTSNVTVILSDERISSPVLNEYDTAIVLNQPSIDKFENSIKPGGILIYDPHGITRHP
                     QRNDIEIYQVEAANKASEAGNMRIFNMIVLGGYLKIKPIVSLDFIEEGLKESLPERHH
                     HLIPANLNAIKIGQEIIKKIN"
     gene            complement(17767..18564)
                     /locus_tag="PKI65_03675"
     CDS             complement(17767..18564)
                     /locus_tag="PKI65_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOB77576.1"
                     /translation="MDVKFESWKEIIKDENIAFKKTKVLTDNILSYCPGCGHGVVHRL
                     VAETIEEMEIQDQTIGIAPVGCAVLAYDFFDLDMQQAAHGRAPALATAIKRLYPEKFV
                     FTYQGDGDLAAIGGNETIHACNRGENITIIFINNGIYGMTGGQMAPTTLEGMVTSTSP
                     YGRDPKLMGYPLRITELIAMLPGTYYVTRQAVYKPAYVRKAKKAIRKAFENQKLNKGV
                     SFVEIVSNCNSGWKMTPVEANKWLEENMLKTFPLGDIKVDGELVKEL"
     gene            complement(18577..19659)
                     /gene="vorB"
                     /locus_tag="PKI65_03680"
     CDS             complement(18577..19659)
                     /gene="vorB"
                     /locus_tag="PKI65_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOB77577.1"
                     /translation="MSEIRLMMGNEAIAEAAIRAGCDGYFGYPITPQSEVLEYLMAEK
                     PYERTGMVVLQAESELAAVNMLYGGGAAGKRVMTTSSSPGISLMQEAISYMAGAEIPC
                     LIANVVRGGPGLGTIQPSQSDYFQATKGGGHGDYCLFVLAPSSVQEMADFVKLGFEMA
                     FKYRNPVMILSDGVIGQVMEKVELIDPIPRRTQEEIIKECPWATTGKTPDRERNIITS
                     LDLDPIRQEKHVMNLKEKFEKMKEDVMWENIQTDDAEYLIVAYGSSARISQKTIDLAR
                     NEGIKVGLFRPITLYPFPEKELERLSKQVKGILSVEMSLGQLIEDVRLAVHDRVPVEH
                     YGRVGGSVHTPFQVLEALKEKIVNKY"
     gene            complement(19687..19914)
                     /locus_tag="PKI65_03685"
     CDS             complement(19687..19914)
                     /locus_tag="PKI65_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOB77578.1"
                     /translation="MAKVKGAIVIDIEKCKGCGLCVEVCPQKVIALGDKVNAKGYFYA
                     DVVNENCTACENCVMICPDTVITLYRAKVEV"
     gene            complement(19921..21246)
                     /locus_tag="PKI65_03690"
     CDS             complement(19921..21246)
                     /locus_tag="PKI65_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOB77579.1"
                     /translation="MSDTIIPQYVLDEVLKKFNVSSLVDTSIRQMCQIVDNLEKETGI
                     QFVRMELGNPGLPPVQIGVDAEIEALKQGIAAVYPDIQGYKPLKTEIKKFVKNFLDID
                     VNIENCIPTVGSMMGSFTSFMTLMHTRKERKKILFVDPGFPVHKLQANVLGSGSEHFD
                     VYNYRGKKLEGILEKYLSTGEFYGLIYSNPNNPSWIVFTDEELRIIAKVSKKYDVVVI
                     EDLAYFGMDFRQDYGKPGVPPYQPTIAKYYDQYILLISSSKVFSYAGQRIGMMVVSPE
                     LFNRHFPDLKTNFNSDHFGTALIFESIYSVSSGTSHSPQYALYAILKAVNEGEYNFRD
                     EVLIYGEKAKIMKKHFLDNGFQLVYDKDVDKPIADGFYFTVKYPGLDGNEFLYQLLLH
                     GIGAISLKITGSDIHDGVRACVSLIPMDMMDELERRLKIMKNNIENLKK"
     gene            21478..22995
                     /gene="dnaB"
                     /locus_tag="PKI65_03695"
     CDS             21478..22995
                     /gene="dnaB"
                     /locus_tag="PKI65_03695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOB77580.1"
                     /translation="MAENSKTSKTTNKNVPKSTNILEPIEIIGGKIPPSDLETEEVVL
                     GALLIEPNAVATVIDILQPDVFYKEAHQRIFSAILSLYPIGEPIDIITVTNYLRRNGE
                     LDLIGGPAYIAQLTDRVISAANIEYHARILIEKYIQRKLIQISNQIITEAYDATSDAF
                     ETLNNAENALFQINEQNLRRSFKSIPSVLQLVREQIEHAYNNQGDYTGVPTGFFELDK
                     LTGGLQPSDLIIIAARPGMGKTAFALSMAHNMAVEFDIPIAFFSLEMSAPQLVIRLLA
                     IETQISSERIRKGELDEAEWETLNNAMNKLSNTKLFLDDTPALSIFELRAKARRLKQA
                     YNVEAIFVDYLQLMSAKIDRNANREQEIASISRSLKALAKELNVPVVALSQLSREVEK
                     RSGTKRPQLSDLRESGAIEQDADIVLGIYRPEYYLKEETDDDDKISEIKNKAEVIVLK
                     HRNGPLGTINLNFINQYAWFTDEDISPIITKRQIFESKINVEVDEDFLNEDNVPF"
     gene            23137..23679
                     /locus_tag="PKI65_03700"
     CDS             23137..23679
                     /locus_tag="PKI65_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOB77581.1"
                     /translation="MTTNEYNKSVDKYGDRLYAFALKALKDDNMAKDIVKESFLRLWE
                     NRDKVKNSKIKPYLFTIAYHLIVDETRYKKRFESLYFTNNDEENEYEIKNNIKDFFHD
                     NCSYHEKIDNFDLKLHINNAINTLPDMQKKILKLRDEDGYSYKEIADYLNISETKVKV
                     YLHRGRQQIKEIIGKIDNLL"
     gene            23676..24446
                     /locus_tag="PKI65_03705"
     CDS             23676..24446
                     /locus_tag="PKI65_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77582.1"
                     /translation="MKKKKFKKLLYQNLNSDKRKDKRYIDLFYQIYNDSKNSNLKLDE
                     SFKESLKIDTNDNIIKKISYSMLKYAVVMIVVLAIGIIAGIGISNNFLTKQEAFLQFP
                     NNYFVIDNIHLKPQTSNELISKNKLSTTILPRDSNKTTINNNDTTNNNLAKIDNLKTI
                     KTSTPIIQVKTEVDTAELIDTKIFYQGLENIARIIEMEDNDLYEIEEQYKKIKTKDLL
                     VVWQDIKNFISDGQVPFNVSVTNIFNLIKNVKIKNQKY"
     gene            24493..24576
                     /locus_tag="PKI65_03710"
     tRNA            24493..24576
                     /locus_tag="PKI65_03710"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24527..24529,aa:Leu,seq:gag)
     gene            24588..24672
                     /locus_tag="PKI65_03715"
     tRNA            24588..24672
                     /locus_tag="PKI65_03715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24622..24624,aa:Leu,seq:cag)
     gene            24794..25855
                     /gene="queA"
                     /locus_tag="PKI65_03720"
     CDS             24794..25855
                     /gene="queA"
                     /locus_tag="PKI65_03720"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOB77583.1"
                     /translation="MKPKKLMHFNFDLPDELIARYPIEERDECRLMVVHKKTGEIEHR
                     IFKDIIEYFDEGDVMIVNNTKVFPSRLMAIKERTGAQIEVILLRELNPELKLWDVTVE
                     PARKIRIGNKLFFGENAELIAEVIDNTTSRGRAIRFLTEMPHDKFLTILKSLGELAVP
                     IILDRPIEPIDNEYFQTVFAKVEGSVLPPFAGLHFTPLLMKRMEVKGINFAEITLHIG
                     TGDLRKIDVQDLARHKTDAEEFIITEEACQIVNEAKKNKRKVCAVGDSVLKAIESSHT
                     IDGFLMPAHRWTSLFIYPPYEFNVPDAYLTNFNLPYSIALMSAATFADYDLIMNAYEI
                     AVKEKYRFHVYGDAMLIID"
     gene            25856..26530
                     /locus_tag="PKI65_03725"
     CDS             25856..26530
                     /locus_tag="PKI65_03725"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HOB77584.1"
                     /translation="MNNNYSIIVAAGASTRMNSQIPKPYIKVNNKPLLYYSLNAFINV
                     MPACNIVVVINNNHKKFYNDFIHEFNEFIDLTYVTGGEQRFDSVKNALSILPDNGFVA
                     IHDAARPFLPISLINNGFALVKEYDSAIPYHQVPNSVRLKQNNNTIPLPRKNVMIIDT
                     PQFFNISLLKIAYEQKFNTDFTDDASVWEKFNLPLYFFQSTHLNIKVTYPEDLILISS
                     ILEKNV"
     gene            26611..27081
                     /gene="greA"
                     /locus_tag="PKI65_03730"
     CDS             26611..27081
                     /gene="greA"
                     /locus_tag="PKI65_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOB77585.1"
                     /translation="METPTYLTKEGFEKLKNKLNKLIHEERPQIAQMIAEAREKGDIT
                     ENAEYDAAKDAQSKLENEISQLQLLLANVKIIEEDEIDKSQVRILTVVTIKNLSNNQI
                     ISYKLVPDKEADLKQKHLGINSPVAQALLGKKIDDIVEINVPAGIIKYKILKIE"
     gene            27084..27488
                     /locus_tag="PKI65_03735"
     CDS             27084..27488
                     /locus_tag="PKI65_03735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOB77586.1"
                     /translation="MSSIFTKIVNREIPAYIIAEDDKYLAFLDINPLAKGHTLVIPKK
                     EVDYIFDLDDETLTGLWLFAKRVAKAIDQYTKDVAIRTGVAVVGLDVPHAHIHLVPIS
                     NVADMNFAKPKLKLDPKEFESIAEGIRSFLKK"
     gene            27742..28464
                     /locus_tag="PKI65_03740"
     CDS             27742..28464
                     /locus_tag="PKI65_03740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOB77587.1"
                     /translation="MAKAITQVLSKHIIGKSKTEVLEMFDAIAPNYDKFTSLFSFGID
                     HYWRESLTLELKKYKPHIILDLACGTGAQAKSLLKLNPQKLILLDASESMIKIANEKL
                     NYPNIEFYHSYAEYIPIATSSVDAVSISFGLRNFENSYMALLSSYRVLKNNGILTILE
                     FGKPNKGFDLLYEIYQNTIMNAFADNILKYQNAFEYLKNTSSEFPYGKDLSAYIETLG
                     FKTLKIRKMTLGIAYLYIFKKK"
     gene            28551..30323
                     /gene="argS"
                     /locus_tag="PKI65_03745"
     CDS             28551..30323
                     /gene="argS"
                     /locus_tag="PKI65_03745"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOB77588.1"
                     /translation="MLIEFLTNTLKKIFTQYNISPHELKISSTEPPHTGDFTFYLFPL
                     LKKTKQNPEQLTETITKILYDNKIIEKHELIKGFLNIQLTNEFLLNFFQTKRENLFDL
                     PQIGTGKTILVEFCSPNTNKPLHLGHLRNIFLGTSISNILQQLDYNVIRINLINNRGI
                     HICKSMLAYQKWFGHETPDNTKMKGDHLVGKYYVAFETELKKQIKKLVAIGISEEEAM
                     KNAPIMKEAYELLRKWESGDPQVLALWEKLNNWVISGFEQTLKRLKVNFDQIDLESEL
                     YKYGKQIILTAYQNGLLERDETGAIFINLENEGLDKKILLRADGTSMYITQDIGIAVY
                     RYQKYCPDQMIYVVGNEQEYHFKVLKSTLKKLNYQWYSIITHYSYGMVELPEGKMKSR
                     EGTVVDADDLLDELKYLAKQTACDSGKLKNLDTNKAEEIYEQIGQGAIRYFILKVDSK
                     KNILYDPKASLDFNGNTGPFIQYTYARIMSLLRKKGIELDYALTLKYEKIDLNDLEKK
                     IILELLNYPNIILDAAKSLRPELIANYAYRLADKYNSFYQNIPVLSEADLNKQNMRLN
                     ISAYTAKVITQSLHLLGIETPEKM"
     gene            30524..31510
                     /locus_tag="PKI65_03750"
     CDS             30524..31510
                     /locus_tag="PKI65_03750"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77589.1"
                     /translation="MDNFNLLDYQALPKEQKRRFLDNLYQFLLENNYTAVDYQKLKIQ
                     STAPICPRCESENVIKAGVRDGKQVYKCKDCGRQYRETARTFVYRMRKADKMLDYLKC
                     MLEGKSLRACASEVGISLRTSFNWRHKILAAIKSLQGGISFSGIVEADELLMKYSEKG
                     RKYRSRKEYEKAKKKKHPKVAVLVMTDREGNLLFKHVGRKKVTNSQIREELSHRVTDS
                     NLICVKQKKEFKKAVKGTKSKKVVVNKTQIRRGVYSVQVVDKHINDFKVWLRRFRGVA
                     TKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSDRWAWDEYWRLVNTKYR"
BASE COUNT        11461 a         4945 c         4611 g        10814 t
ORIGIN      
        1 tttatttttt gcaaatttaa taaattttct gatattaatc ctgtataaat tttaaaaatg
       61 tatttttatc cttaccccat tactatcaaa acaatataga aatattatta gctaaaattc
      121 aattataaat ttttaaatta tgatatcata atttaaaaat tttgtatatt tgtatgttat
      181 gaataattta tttggattaa gtgaagaaga aagtaaatat cttgttaata gatatagata
      241 tctactacat actcttagat ataatacaag caaggaaaat agagcactta ttagaaaagc
      301 atttaattta gctagtgaag ctcataaggg catgaggcgt tatagcggag atccttacat
      361 ttatcatcca ttagaagtgg ctattatttg ttcagaagaa ataggattag gcactacttc
      421 tattgtaagt gctttattac acgatgtagt cgaagataca gattataccc tagatgatat
      481 aagaaatcat tttggtgata aaatagcttc tatagtagat ggattaacta agattacaga
      541 ttttattgaa ttatctaaat ctgatgatac ttctattcaa gctgaaaatt atagaaaaat
      601 acttataaca atggcagatg atgtaagagt cattttaata aaactagctg ataggcttca
      661 taatatgcgc acattaaact ccttgccgcc acataaacag caaagaatag cagccgagac
      721 gtccgttata tttgctccac tagctcatag acttggattg tataatataa aaagtgaatt
      781 ggaagatctt gcattgaaat ataatgatcc tgaaatttat aattatatca agtataaaat
      841 agaagaaaca tcttctgaac gagatgcatt tattaaagat tttattaaac ctataaaaga
      901 acgtttagat caaactaatc taaaatataa aattacttgg cgcacaaaag ctatatcttc
      961 tatttataga aaaatgactg aaaaacaatt gaacttcgaa gaaatatacg atgtatttgc
     1021 cattagaatt attttagata gtccaccaga aaatgaaaaa tctgattgct ggctggttta
     1081 tagtataata actgatattt atactatgaa ccctgaacgt ttacgtgatt ggatatctgt
     1141 acccaaagca aatggatacg aatcattaca cgtaacatta atgagtaaat caggtaaatg
     1201 ggtagaagtg caaataagat ctaaacgaat ggatgaaata gctgaaaaag ggtatgccgc
     1261 tcattggaaa tataaagaaa ataatggtaa aattagtact ctagatgaat ggttaaattc
     1321 tattcgtaat atcttagaaa atcctaatga aaatgctttg gattttttag atgatgttaa
     1381 attcgcttta tttactgatg aaatacccgt tttcactcct aaaggcaagc tattacaatt
     1441 gccaaaaggc tctacagtct tagattttgc ttacgctatt catacagact taggtgatca
     1501 ttgcataagt gctaaagtaa atcataaaac tgtacctgtt aatacagtat taaaaagtgg
     1561 agatcaagta aatatattga caagtgaaaa aagtagccct aatccagaat ggctaaattt
     1621 cgttaaaaca agtaaagctc gtaatgctat taaagctgct ctacgtaaac aagaattagc
     1681 tctcgccgaa catggcaaag aaataataga aagaatgttt actcaattga aagaaaaata
     1741 taatgataat ttattacaaa aatttgctaa ttatcttgga tataataata ttaaacaatt
     1801 atatattgcc gctgcaaaag ggcaatttga cgttaaacaa ttaaaacaat ttctacaatc
     1861 tcaaaaaccc tctacatttt ctaggctttt tagacattat tccaattcta ataaaaatag
     1921 ttatcaaagt aatgaaattg aagaaaaaga aaaaattcaa actaaaatta ttcttgatac
     1981 aactcaacaa ataaattata ttcttagtga atgttgtagc cctataatgg gagatcagtt
     2041 gattgggtat atctcagatc ctcatactct cgaaattcat aggactactt gtcctgtttt
     2101 taaagaaaaa gctgctgtac acggcgatag agttacaaaa gtaaaatggg aagaaaatag
     2161 acctctcgaa tttaaaacaa gtatagaact cgaaggaagc gatagaaagg gcatagtaaa
     2221 agatattagt aaattaattt cagaagaaat gaattctaat attagatatt ttttccttga
     2281 agtaaccgaa ggtactttta aaggcgagat caaattatac gtaaataata ttgatcatct
     2341 caaacaacta attgataaaa ttaaaactat cgatggagta agtaaagttt atagaaaaga
     2401 gtaataaatt taatacaaaa ttaatacgaa attttatttc tgctagataa aataattaaa
     2461 ttaatctatc ttgacaacaa taaaatcata aaaaatcact aaaatcttct ctaacaactt
     2521 attaattttt tttctctctc ctaccctatt tcgccgttta ttataacttt atctactttg
     2581 tttgaagcaa aatgataagg aaagaagttt agatttgtaa catctgaatt gagtatcaaa
     2641 aaagatgctt ttttaccttt ttcgatacta ccaagttgtt tttctagctc aagcgcaaat
     2701 gctgcattat atgtaacagc attaatagtt tcctctggca tcaatttttg ataaatagta
     2761 gctagcgaaa ttgcaaattg caaattacca gaaggactcg tgccaggatt ataatcacta
     2821 gctatcacta ttggtaatcc agcatctacc atcttgcgag ctggtgcata aggaattttt
     2881 agaaaaaaag atacctgagg caaaactgtt ggtagagttt tacttagcaa caatgctcta
     2941 atctcctcat ctgttattcg ttctaaatga tctaccgaaa gagcattatg cgctatgcca
     3001 agctgtacac cgccattatt gctcatctca cttacatgta ttttgggttt caatccaaat
     3061 ttgttagctg catttaatat tttatcagtt tcatctatgg taaaaaaacc ctcatcgcaa
     3121 aaaacatcaa cataatcggc aagtccttcg tctactacct ttggtagcat ttcactaatt
     3181 agcaaatcta tatatccttc tctatcatct ttatattcta taggaaaagc atgggcacct
     3241 aaaaaggttg atttaatcgg cagattggta tattctttta attttttaat cacttttaac
     3301 atctttagtt cactctctaa agataaacca taaccacttt taatttctaa tgccacagtt
     3361 ccataactta taacttcatt aattcttttc aaagctaagt caaagagttc attttcagat
     3421 ttttcttgta cttttttagc agaattcaat attccaccac cttttttagc aatttcttcg
     3481 tacggtatac cttgtatttt cattataaat tcatcttctc gccacgaagc aaaaactata
     3541 tgagtatgtg tatctatgaa tcctggtatt attattcttt tatttacatc ataaatatta
     3601 tcataatttt tataaattgc agaaagctct cctagctcac tcatcatacc ataatctatt
     3661 atagtatcac cctctacaga tagataagca ttttctattt tatctattct actcatttca
     3721 gctcctttta atggtctatt aggataattt tcactaatac ccatcaatga accaatatta
     3781 acaaataatt ttttattatt cataataatt caatttttta gcaaagataa tgttttgaaa
     3841 tgaaaaaatt aataaaatta catagcaagc gaaaatctaa tactaattcc aaaattagta
     3901 gatgtattat aatattgtga agctacatgt ggcttattaa ttatcttatc aaagaaagct
     3961 ctaatagtaa tattattggt tatctgatag tctgcagata aatttataga aataattttt
     4021 tgaccagtgc taacctgatt tatctcctcc acaatacgcc ttagtactgt aatattatct
     4081 ctaatagata gatctgcttt taaatttaag tcactattaa tagttttagt ttgtccacct
     4141 gttctaatat caaaggctaa atctttaaat ctatacccca gtcctataac ccattcatta
     4201 gtattgattt ctgtaatttg tacattagaa gtattcaaag caatatttcg agattttttc
     4261 caatcaattg tagtcactaa actatttttc caatttaact gaatgccaaa cattgggtta
     4321 aaagtttctg aaatagaaac ctgagctatc tcatattgtg gcaaataatt atgaagtgca
     4381 tctctcacag tagtataacc atctccatca ggatcagaaa acagaggatt attagaaaaa
     4441 gaagataaag tgtaattaga tgtataaccg tgggtgagtg tagctgaatt gaaatatttt
     4501 tttaaaaatt ctattttaga aagaccagta tagcttatac gccaattagg cataggaaat
     4561 gatggaaaaa tagactttac atattttgat ggcttatgac cagtatatgc agctaaaaaa
     4621 gctggaatta acacattttg agatgttgct ccgtatccgt ctggatataa tatcccagta
     4681 actgaatctg ctacataagt accgtcccat acaggactac gttcggctac catctgtgct
     4741 atttctggac gaatatccaa aagtatttga aaattcttat ttgaaaaatc tttagcattt
     4801 ttagcaaaag ctgttttact actgatgata gacatactaa aactacctgt agttagagga
     4861 gtataagatt gaaattcact accattatat ctaaaatatt ctctattatt cattgattta
     4921 tttctaatag cagtgagatt tattgttaaa tatttaattg gttctaaggt aactctgata
     4981 ttcatatttt cagtatgatt agtaaggtat ggtgtattta aggtggtatc tttagtaaat
     5041 aaatccttag caacaagtct atcacgcaca tcctcttgcc aaccaaagac aaatccccaa
     5101 ccaggtgtca tattattgaa ttccatacct aatgcttgag gtacccctct ataacctgta
     5161 agcatagtag catctgaaaa agaaatgttg aaactaacat ttttggtcat cattaataaa
     5221 cgtaaaaaat tatctaaaat ctgcttacca atggttggtc cttgtttaat agtatcttct
     5281 tcatcttgta gacgaggagg tggcggtgga tttaatatat tttttaaata tgtaacctta
     5341 gtatataatc tagtaaaatt accattaaca ttaaatgata ttgtacggct attttgtata
     5401 ctattaccat atggatgctc cacatattgg tcagtagagt ctttatatag cggagcagcc
     5461 atccatgtat aattaccagt gtactgagct gaagcgttca tccagtctaa tataggtata
     5521 taatttaatg gcaaagtata attgccttga attctttggt tgaaattagt aattctacca
     5581 aaattaacta tatttctaag tatagaatct cttttttctt tataatcact ttctcgctta
     5641 tcgattctgc caggtggctc atctatacgt gcgttagcaa ctgcagtata atcaaacctc
     5701 agattacgtg tgagatcata tttgaatgca taattgcgat tccacataaa atttttaata
     5761 aaatttggtt ctattatcac aagattagta gtcttatttc tccatctatt ttcttcatat
     5821 aatcgataca tatctgtaga caaagagatg gatttgggta gcaaataaaa attaaaatta
     5881 cctattaatt ttaaatattt attattaaga aattttacat ttttgaaagg catataattt
     5941 ttaggattac cattaaaatt atatgctatt gtaccactat attctttttt cttattatac
     6001 tcaatatcag gattacgaat ataaatttga ttaaaagcaa aagaaatatc aaaattagat
     6061 aaatcataaa atttgggatt gcctttagca gtaggaggtc ttaatttttt tacattcata
     6121 aaattaatac ccttacgctg agtatatgat tgcactagtc gttttacact atctacttct
     6181 ctatcggtag catatgtact gaggtcatct ttaagataaa catctgggtc taatggatta
     6241 tatttaggat tagctatcat ttcagaatag tctatatgca tagggatacg taaataaaaa
     6301 ttttctggca aaagcctacc taagtctata tttaaagcta gatcatacga tgatatatta
     6361 tctaattgac gttcataaac ttttttttca atagaaccaa agccagcagt agataatgta
     6421 gtagcgaaca taatattacc aatgtcagct aaaccaatat tcatacgagc agtagctgcc
     6481 catccacctt tttcgtcaaa agtatttacc cttaattcat ttacccatat ttcagcacat
     6541 ttgggcattc catcatcatc ggtataagta ccagctttcg gatttcgcac tcctatcaat
     6601 ataactttta cttcagctag attaggatta ccaacgacag tatatttacc tttttcatct
     6661 tctttagaat atggtgtaaa tagtgttaga ttagtattgt cttctccacg aagtatagta
     6721 tttcgttcta atttcaactc taccaatttg tctaatgata gatcaataga atctgcccat
     6781 atacgtttgc gactttcatt atcatgccca taattacccc atggtgtagg atgtagaggt
     6841 atttcaaatt cataataatt tctatcaaaa tcagtaccta atcgtataaa caatgttaaa
     6901 tcaccatcct ttaacgaata attcaatgga tccacctctt cagtatggac attcatttta
     6961 atttttttat aatatctaaa atcataatca gtacttttaa aaataccccg agcatctcca
     7021 tctattagat gacaaacttt catagataaa gcttgttcat ttaatttttg taaatttgta
     7081 gtaccataat taatctctct ctcgattcct ggtggcataa cgtatggtat gggtttgcgt
     7141 gtgccgtttt cctcaatatt caaattagta atatcgaatg ttgtcagatt ttgctcattt
     7201 tctggaatat attcaccagg ctgcagtaat gaatatgaat aacggcgcca ttgactacga
     7261 gctagatcta aagaaccgaa acgtaaaacg acagaatcat tccattctct caaaaacatt
     7321 ctcataaaac gaatagaagt aaaatctcta atatctccaa ctattttatc aggtcttttc
     7381 actggtactt taaattgata ccattttact tgttcagaat taccatttgg taatttaaca
     7441 cttgcaatgt aaatatctgt aatataattt ttgccaattt ccatttgatc aggtctgaga
     7501 tttatttcat attgataata cctttctaat tcatttaaag tattgtctct attaatgtct
     7561 tcactattag gattattagt agcgctagta ggataagact ctggactcat ttcaggtgta
     7621 gggctattag cttcaggatg cctataatat ttgtatcttt gcagaatatt ataacccaaa
     7681 ttatcataat ctccgcctct aaaatagtga taattgtcgg cagaaggatc ctgataagct
     7741 aattgatatg cgatggaact agcaccataa atagcttcga tagcttgtaa atatgatgaa
     7801 tgaaaacttc tttcattttc atctgacaaa ccatcataac caacatcttg atattttcta
     7861 gcatctagat tagcatcaaa agagtttact aatgcttggt atttaggtac tactccccat
     7921 tgagttgtat ctatacctat catttctgga ccagtaggta gcccgtgctc gaaagctttt
     7981 ttatgatcta ataataaatc ttctgatata tctcccaggt ttatatacaa tttaccgcca
     8041 ggacttgcag gattataaat aaatggatcc aacaaccaaa attcaatata ttctacatta
     8101 gtttcttcga aattcggtgt ttctattgtt cgcataatac caccccaacg agtttcggga
     8161 tctgctaaac gcccattagc atctatacca gcagatacat tagtaggtac tatatcatag
     8221 ttataaggtc ccctctcact aggataatat gctaaatcaa gagtagctag attaggtact
     8281 ataccattag gaaattcttt agctgggaat aattcagact ctaatatttc gcgcatataa
     8341 tgattactga gcatttcttt attcacatta ggcggagtag tagtgttatt tctaaaaaac
     8401 acactagggt ctattgtgta ccaagataat aaagctctat tataaccaga tgtaagggta
     8461 gtgtcatcac tagcttctgg aaaaatatta gactgtcctt gaggtataga tgctaaatgc
     8521 catgcaaatg gatttttcaa agtaatacta gtcatgctgc cttcaaaatt atcgataaaa
     8581 gcattaccct ccttacctat ggctctatta tgtccaggaa tgagattggc tacttcggca
     8641 tctatctgaa tagaagaagg tgttttagtg cttacaaagg gcaatttatc tataatatta
     8701 gtaattaatc ttgactctgt ttgataagaa atatttgcac cccatatagt gttagatatt
     8761 ggttcatatc caatatttac tttttgtgta aaaggttttt ctgataaatg aataatagta
     8821 ccacctacaa tgaaattatt attaatttta taatctacgt gtgctccaag gtaagtcttt
     8881 gtttgaaaat taaaaaaagt attattttct acagttacat ttattgggac tccagagcta
     8941 agtatacctt gattaatgat tttcacacgt cccatagtat agtctactgt ataatctaca
     9001 ttctcttgaa gcaaaatacc accagctgtt actttcactg aaccttgagg tatatttatt
     9061 gcatttagat ttatttcatt tcctacactc gatttatatg atccttcgat agtaaatcta
     9121 tttttattag ctatttgcat agctacagtt ttagtagtag tatagagcga atcgaaacaa
     9181 tatttatctg ccatagcagg gtcatcaaat ttagtcctta gatctttacc aaatggctct
     9241 attacaggga aataaatacg accattccta gaattaatag tgccaccaga ggtagctgca
     9301 ttatctaaaa agtcaaaaag tccatccggt tcaggatcac cattaatgtt taatttatca
     9361 aaacctaaaa ctctaattaa tgggataaat tttaaattag attctgtcaa atatccagcg
     9421 cctacaccta aattttcatt tttatgaata atatttagtc taaaatcttc gttactaatt
     9481 tgataaccac ctatagaata aacatttttc atcatcaaat tccatatggg taatttagta
     9541 ttaatagatg atggttttaa taatttcact actagacaat taggcggatc tattccatct
     9601 gtagagaatt cccctacttg atacacacta tcactaccta taactgtata ttgataagcc
     9661 acagctaaaa cttgatcggg acgtaaacta atattaagag agataaatcc taattgatta
     9721 tttacagagt attcagatgg tgatagctta cgagcgtttt ctattttttc ataatcagta
     9781 cccattttca aattttgagc agaaagatat gaatttacat cattcaaatt tctaatattt
     9841 gctggattta ctatttgtag taaattattg cttctattac taggtaaata agcatctgga
     9901 gtgaaattag gtaaaatatc ttgattatat ggctgatatt ctcccaaatc ttgaaatgct
     9961 acgatatttc tattttcttg agtagcagga ccaatatttg taatccatac ttctatttta
    10021 gtaatgttta cagggctaga aattattggg agtcgttcta aaaatttgtt gtaattatta
    10081 taaaaatatt gagagataaa gaagtgttta ttttcttcgt aatcaagagc ttcgatttta
    10141 aattctctca cctctgcacc gccagctaca gtaatagaac tagtttgact ctcttgctga
    10201 gaaagtacag ctgttacagt agctttgcca aactgtaatt gagtttttag accaaataag
    10261 ctttgactac cacttatcaa ggtgctatta agtggcaagg ttacgtctcc agcttctatt
    10321 tttttgataa tttcatcctc tttgccctca tattctaact ttattttatt atcaaagtca
    10381 aaactagatt ctgtattata tcttacattg aagtttacct tatcacctac ttttgccatt
    10441 aagtttaatt gaattttttc ttgaaaatct aaatttccat actttctttg cttaacagta
    10501 agtgatgggt catcccttct ataacccttg tatccaaaaa ttatttcagc cgtaccacta
    10561 gatctgatgt ctattagtcc actaccaaag actccctcat cttttacatg catcctaaaa
    10621 agcgaatcaa ataaactttt gcctttagat tgagaagtta tttgtttttg ccattctctt
    10681 tgccatgctt tttgaaaatc atattcagaa tattcatcaa aagtcatctc ataaggatag
    10741 cgcaatttaa tatttccgat atgttgttga tattcataag tattagtaga tggatcatag
    10801 atgatagtat ctttcaaatt tttaggattt acatcccatg gcgaaattat caaaggtgga
    10861 ggtcctatta tttgctgatt gccaatagga tatttcaaag aatctaaggt atctgggggg
    10921 cctggaaaat gctgaatatt taactcatta cttacataat aatatttcgg tgaaaaagaa
    10981 taaaatataa aaaatgaaaa tatagataat acaaatatac atctaataaa aggtatagaa
    11041 tgagttgata tttttttcac tattattaaa gtttataaat acttaaaagc taattttatt
    11101 agtttatcca aagaaacata ctcttccttg gaatgatttt ttaaaacttt attcaatgcg
    11161 ttttcggctt ctttcacaga ataacctaat tgttttaagg ctaataacgc atcttgataa
    11221 aaattagtat caaaattaga taaatttttt aaactttgtt gaatttctgg gattttattt
    11281 tttaattcta ttattattct ttcagctgat ttaataccta taccttttac tttttgtaaa
    11341 ctagtaacat ctttgcttag aatagccgaa tacaaatcta tcggtgtcaa tgtagataat
    11401 agattacgag ctgtattagg tccaatgcca ttaactgtaa tcaataatct aaaagcttct
    11461 ctttcatctt catctgcaaa tccatacaat tgtataccat catctttcaa tattaaatgt
    11521 gtcaaaactg ttatctcttt agattcttta atcttttcaa aagtatttat agtgatttga
    11581 acctcaaagc ccatgcctaa agattctatt attaccttag atggatttat agaaactaat
    11641 aaacctttta tataattaag cataaaaaga tgattttttt taaaaaaact aaggtccaaa
    11701 gttaataatt attaataaaa tgctaagcaa tattttttaa aatatctaaa ttaaaaaaaa
    11761 atgttattta tcaattctga tttgaaaaat tttcaataat tttgtataaa ttataaatat
    11821 gccaaaatat cctttattag ctaaaataaa tggtccagag gatttgaaaa aattttctct
    11881 caaagagctt aaatctatag ccaatgagat tagagaatat attatagagg tgatgtgtaa
    11941 cactcctgga cacgttggag caaatttagg tgttgtagag ttaaccattg ctttacatta
    12001 tgtttttaat agtcctgatg ataaaattgt atgggatgta ggtcatcaat gttatccaca
    12061 caaaataatt acaggacgta gagaagaatt tagaactatt cgaaaattta aaggtataag
    12121 tggatttcct acaagagaag agagtattca tgatgtattt ggcacaggtc acgctagtac
    12181 tagcatatct gctgcactag gaatggcttc tgcagaaaaa attattaatc cagatagtaa
    12241 aaataatttt atagcagtga taggtgatgg tagcctgaca ggaggtatgg cttacgaagc
    12301 tataaatcat gctggcacta ctaacactaa cttcttaata atctataatg acaataatat
    12361 ctctattgac gaaagcactg gagctttaaa aaattatttt acacatttta tagcttctaa
    12421 atcatataac caattaaaaa acactgcata cgaacattta aaaggaaccc taattcgtcg
    12481 attttttaat aaaatttcat ctattataaa aaatacttta ttaaaacatt ctaatttttt
    12541 tgaattcttt aatctaagat attttggtcc agttaatggt catgatatag aacaattggt
    12601 aaaaattttg aataatctga aaaatatacc tggtcccaaa atattacatg tattgactca
    12661 aaaaggtaga ggatacccac cagctgagaa aaatgctgtt gcatttcata gtccaggtaa
    12721 atttgaccct gaaactggtg aaatttttag taatagaaat gacaaaaaca gtcctttaag
    12781 gtatcaagat gtttttggca atacaatatt agaactcgca gatatggatg atagagtcgt
    12841 agcagttaca ccagctatgg tatctagctc gtcattagac attatgtatg ctaaatatcc
    12901 aaatagagta ttcgatgtgg gcatagccga agagcatgct gttacttttt ctgctggttt
    12961 agctgcacaa ggtctgatac cattcgctac catttattcc acatttctac aaagaggttt
    13021 cgaccagata atacacgatg tagctttgca gaatcttcca gtcatttttg ctatcgatag
    13081 aggcggcata gtaggagagg atggcaaaac tcatcaggga gtttttgacc tagcatattt
    13141 gaatttaata cctaacatga tcataagtgc tcctatagac tgtatcgaac ttagaaatct
    13201 aatgtatacg gcctataaat acagaatggg accttttgct attagatatc ctaaagctat
    13261 tgctgagttt gtaaattttg ataaaccttt tgaactttta cctattggca aagcacaaat
    13321 actaagagaa ggtgaaaaag ttttgataat ctctttgggt aacccaggac atgatgtcgt
    13381 agaactttac ccagaatttg ataacctgga aattaatcct actcatgtta atctgcgctt
    13441 tctgaaacct tttgatgtgg atactttgaa ccaattatgc aaaaatcatg aaatcattat
    13501 taccatcgaa gatggtactc tatatggact ctcctctact atatcagatt ttttagttaa
    13561 aaataaattt aataataaac ttattagctt aggtgtacca gatcaattta tagaacatgg
    13621 cagtataaat gagctgaaac aaatgctaaa atacgataaa aacagcattt taaatacagt
    13681 aatacaagca tataaaggga tatacaaatt gagctaaaaa aatttttgaa ttatcaaaaa
    13741 tttttagaat tttgtaaaaa aacaactatt atgaaaaagg cagtacatat tgttagaata
    13801 ttgataggat tactttttgt tttttcaggt tttgtaaaag cagtagaccc atggggtttt
    13861 caatttaaat tagaagatta ttttattgct tttaattggg acttttttat gccttttgct
    13921 ttgtttttag ctattttggt accagcagct gagtttatca taggttttgc ccttttatta
    13981 aatttaaaac ctaagtatgc tatctggggg gctctcatct ttatggcatt ctttttgcct
    14041 ctcaccttat atctagctat tactgatgca gtatcagatt gcgggtgttt cggtgaagct
    14101 attaagctaa gcaattggaa tactttttat aaaaatataa ttatcacagc agctattatt
    14161 tttgtcatct ggcaaagaaa atatttaaaa tcatggttaa aaaatgctgt agagtggata
    14221 ttaatagcag tgtcaggact ttttataata ggagtctccg tctatggatt gacatacctg
    14281 cctatcgtag actttttacc atataaaata ggcgtagacc taaaaccaga tcccaatcta
    14341 aaagataaat atttcgtaat atataaaaat aaaacaacag gacaattaga agagtatcct
    14401 gccaatcaat atccttggga tgatagcttg tggatggcag aacacgaatt cgtagatact
    14461 cgagtagaga aagcagctaa atcagccctt ttgatattaa cctatgataa tgatggcaat
    14521 gacgttactg atagcgtact gctaagtaaa aaacctacaa tgctgatagt tagttatgat
    14581 atcaaaaaag taaatcctaa aactgccacc aaattgaaag aatggataaa catagctaat
    14641 aaatttaatg taaatacatt tttaataaca tcctctgatg agaatttagt aaaaaaacaa
    14701 ttaaaaaata gccaaatcga tataccctat gtttttgctg atgatatagc actcaagatg
    14761 cttataagag caaatcctgg tattgtaatg atgagagatg gtgtgataaa agctaaatat
    14821 actttaaatc ataaagctaa tgaaaaaaaa tttgacaaat tattaagata ataaattttt
    14881 taataaaatg atcaaaataa gtgaaaggct gggagaaagt gttatgaaaa gacaaggtat
    14941 aaataaaaaa ttgagaaagt tattaattgg agtttaatag ttcttatctt tttcctatct
    15001 gataattgct attttagatt tataaaacat cgtttttgta ataattataa ccaaaaagag
    15061 caagatcata tttaatcgga tcaaatggat ctagttgttt aagattataa gttagttcta
    15121 ttactgcttt taaatcattt tgctttcgtg acaaaagtcc taattcgcgt gcagccatac
    15181 caacatgcaa atctagaggt atatagagag atgatgatgg gatattttgc catatgccaa
    15241 aatctaaacc ttctttattt gtgcgtacca tccatctaaa atataaattt attcttttac
    15301 aagcactatt gttgtctgga ttaggaatat gtttttcgct acgctgctca tgtggtacaa
    15361 acaaaaaaat atcccttata gctcttatac tgtcgattaa atctctattt ttatcataat
    15421 taatttttac aaaattattt aaactatcat atttgaaata tatcaaacgc agggcatcta
    15481 agaaatagaa taaatccatt ggctgaaaag tacgataaac aaaagattca caagagttcc
    15541 aattcttttt attaccagta atgaatttat agggttgata attcatcaat tgcataagat
    15601 ttttggcggc tttcacaata ttttcgcgtt tcccccaagc aataatagca ctaaaaaaac
    15661 ccgctatttc gatgtctaaa ggatctgaaa atatagcggg aactattatt gggtctactt
    15721 gccaatattt tggttgagaa aattcctgat aataagtatc taataaagtt ttaatattag
    15781 ttttcaattt ttacaatatt attaattaaa attaaaccct cgtataaata tatgatgagc
    15841 ttactaaaac tagttgttta ttatttaagt cctttttgtt ttatttgtga aattacttct
    15901 gttaaaatat ctaaaaatgt tgaaattgtt gtattatcca caggtttatc aataatccat
    15961 ttataatttg atcctgcggg ttcaaagaac tgtgttttta tcaactcttt tttgaaataa
    16021 tctataatag tttcagcatc attcactttt ttatcaattt gttctattac actgtaaata
    16081 ctttgtttaa atacagattc aggtgggtag cccaaacata atccaacaaa actgctctca
    16141 atgggcaaat ttaaagaaaa acctttagaa ccccacctgc atgatagtcc attactttca
    16201 gcaaatttga atattctttc aaaaacttct ttaccatttt catctaccga ttctagaaat
    16261 gttatttcat taactttggg taattgagtt ttagatttta cttctccttt tatgtagctt
    16321 tcctctccaa taatgaaatc acatgttatc attttttcta aagcattatt ctgaaaccat
    16381 ttaaactcta tacaataaat atcaactcct ttttttctta aataaagtgc ggtttgctta
    16441 atattatttg ttatatcttg tgctacaatt actaattttg tatttttatt aaatgataca
    16501 ttagcatcac cagtgatttt aaaatattcc tgatgataat tatctaatga tatttcttcg
    16561 ccaacgtatt cttggaatat ttcatttaac aattcatagt caagattttc tacaaaagac
    16621 gcatattcta gaagttgcgc caatgtttcg cgtggtgttt tatctctttt taattcgatt
    16681 ataatagtat tgccattttt atcgagtcct aataggtcta tcaaagaacc taaatttgtt
    16741 tgtacctgac ggccaattat cattatttca ccatcttcga aaaaatattc agggtttttt
    16801 tcaagtaaat tttctaaatc aaattcattg ttttcttctt taaaagtatg ttctttaaat
    16861 ggaattaatt ttccttcatt atttattgta aaaagtctca taattcttat attcgctttt
    16921 tttatttatt aatgcatcaa ttacattaca aatatacaac tttgttaaat acaaaaattt
    16981 aaagctgatc aattttttta attttttgat aaaaacgaat ataaagaatt ataaaaatta
    17041 ccttattaaa ttttttttaa ctccaaaaac ttttgaatca gagtgtttgt aatttatctt
    17101 tcccacatac tttacctcat tgttacattt cgcttagctt tttatttcac tatgagataa
    17161 aataccaatc aaatggtttt tgtaataaat aatagtaatt aaaaaaaact aattaatttt
    17221 ctttataatc tcttgaccaa ttttaatagc attcagatta gctggtatca aatgatgatg
    17281 acgttcaggt agtgattctt taagaccttc ttcaataaaa tctaaagaga cgataggttt
    17341 tatttttaga taaccaccaa gtactatcat attaaaaatt ctcatattgc cagcttcgga
    17401 tgctttatta gcagcctcta cttgataaat ttcaatatca tttctttggg gatgtcgagt
    17461 gatgccatgt ggatcataaa tcaatatacc acctggtttg atactatttt caaatttatc
    17521 aatagagggt tgatttaaaa caattgcagt atcatattca tttaatactg gactacttat
    17581 acgttcatca cttaatataa cagtaacatt agatgtacca ccacgcattt ctgggccata
    17641 agaggggaac cagctaacat ttagaccttg tttcatgcca gatgttgcta aaattttacc
    17701 catagattgt acgccctgtc caccaaatcc tgctataatt atttcttctg tcataataaa
    17761 acaattttat aattctttaa ctaactcacc atccaccttg atatcaccaa gagggaaagt
    17821 ttttaacata ttttcttcta accatttatt agcctctaca ggggtcattt tccatccact
    17881 attacaatta gagactattt ctacaaacga aacaccttta tttagttttt gattttcaaa
    17941 agcttttcta atagcttttt tagcttttct aacgtaagca ggtttataaa cagcctgacg
    18001 agtaacataa taagtgcctg gaagcatagc tattaattca gtaatacgta aaggatatcc
    18061 cattaattta ggatccctcc cataaggtga agtagaagta accataccct ctaatgtagt
    18121 gggagccatc tgtccaccag tcattccata aataccatta ttaataaaga taattgtaat
    18181 attttcaccc ctattacagg cgtgtatggt ttcattgcca ccaatagcag ctaaatcacc
    18241 atcaccttga taagtaaaaa caaatttttc aggatagaga cgcttgatag cagtagctaa
    18301 agctggagca cgtccgtgag cagcttgttg catatctaaa tcaaaaaaat cataagctaa
    18361 tacagcacaa ccaacaggag caataccaat agtttgatct tgaatctcca tttcttctat
    18421 tgtttctgct actaatctat gtactacacc atgtccacat cctggacaat atgacaaaat
    18481 attgtctgta agaactttag ttttcttaaa ggcaatattt tcgtctttta ttatttcttt
    18541 ccaactttca aattttacat ccataatttc aactctttaa tacttattaa caattttttc
    18601 ttttaaagcc tcgagcactt gaaaaggagt atgcacggac ccaccaactc taccataatg
    18661 ttctactggt actctatcat gaacagctaa acgcacatct tctattaatt gacctaggct
    18721 catctctaca gataatatac ctttaacttg tttagaaagt ctttcaagtt ctttttcggg
    18781 gaaaggatac aaagtaatag gtctaaatag tcccacttta ataccttcgt ttctagctaa
    18841 atcaatggtt ttttgactta ttctagcact agaaccataa gctactatca ggtattcagc
    18901 atcatcagtt tgtatgtttt cccacataac atcttctttc attttttcga atttttcttt
    18961 taaattcatg acatgttttt cttgtctgat aggatctaga tctagagatg taatgatatt
    19021 tctttctcta tcaggagttt ttccagtagt agcccaaggg cattctttta tgatttcttc
    19081 ttgagttctt cgaggtatag gatcaataag ctctaccttt tccattactt gtcctatcac
    19141 tccatcagat agtatcataa cgggattgcg atatttgaaa gccatttcga aacctaattt
    19201 aacgaaatct gccatttctt gtactgaaga tggtgctaaa acaaacaagc aataatcacc
    19261 atgtccacca cctttagtag cttgaaaata atcactttga gatggttgaa tagttcctaa
    19321 gccaggacca ccacgtacca catttgctat caagcaagga atttcagcgc cagccatata
    19381 agaaatagct tcttgcatta agctaatacc aggactagaa gaagtagtca taactctttt
    19441 acctgcagca cctccaccgt atagcatatt tacagcagct aattcacttt cagcttgcaa
    19501 aaccaccata cctgtgcgtt catatggctt ttctgccatt aaatactcca aaacttctga
    19561 ttgaggagtg atgggataac cgaagtagcc atcgcaacca gcacgaatag cagcttcagc
    19621 aatagcttca ttacccatca ttaaacgtat ttcactcata tttattaacc ttttttttta
    19681 aaaaaattaa acttctactt tagctctata caatgtgata actgtatcgg gacaaatcat
    19741 aacacaattt tcacaagctg tacaattttc atttactaca tctgcataga aataaccttt
    19801 ggcatttact ttatcgccta aagctattac tttttgaggg cacacttcca cacacaagcc
    19861 acaaccctta catttttcta tgtctattac aattgcacct tttactttag ccatattttt
    19921 ttattttttt aaattttcta tgttattttt cattattttc aatcgtctct ctaactcatc
    19981 catcatatcc attggtatca atgaaacaca agccctcaca ccatcatgta tatctgaacc
    20041 tgtgatcttc aatgaaatag ctcctatacc atgtaataaa agttgatata aaaactcatt
    20101 accatctaaa cctggatatt taaccgtaaa ataaaaacca tcagcaatag gtttatctac
    20161 atctttgtca taaacaagtt gaaatccatt atccaaaaaa tgttttttca ttattttggc
    20221 tttttctcca tagataagca cttcatctct aaaattatat tcaccttcat ttacagcttt
    20281 taaaatagca tataaagcat attgtggaga atgactcgta ccactactaa cactgtatat
    20341 actttcaaaa atcaatgctg taccgaaatg atctgaatta aaattagttt ttagatctgg
    20401 aaaatgtcta ttaaataatt ctggactaac taccatcata cctattctct gtcctgcata
    20461 agaaaaaact tttgagctac tgataagcaa gatatattga tcataatatt tagcaatagt
    20521 tggttgatat gggggtacac ctggtttacc ataatcttgt ctaaaatcca taccaaaata
    20581 agccaaatct tctataacca ctacatcata ttttttagat actttggcga ttatcctcaa
    20641 ctcctcatca gtaaaaacta tccaagatgg attatttggg ttactataaa taagtccata
    20701 aaattcacca gtagataaat atttttctaa aataccttct aattttttac cacgataatt
    20761 atatacatca aaatgctcag aaccagagcc taatacatta gcttgtaatt tatgaacagg
    20821 aaatcctgga tctacaaaaa gtattttttt tcgttccttg cgagtatgca tcaaagtcat
    20881 aaaagaagta aagctcccca tcatagatcc aacagtaggt atacaatttt ctatattgac
    20941 atcaatatcc aaaaagtttt ttacaaattt tttaatctca gtttttaatg gtttataacc
    21001 ctgaatatca ggataaacag ctgctattcc ttgtttgaga gcttctattt ctgcatctac
    21061 tcctatttgc actggaggca aaccaggatt acctaactcc attctgacaa attgaattcc
    21121 agtttctttt tctaaattat ctactatctg acacatctga cgaatggaag tatctactaa
    21181 agaactaaca ttaaattttt tcaatacttc atctaataca tattgtggaa taatggtatc
    21241 tgacataatt gttttttgca aatctaataa tttttaatta aataacaata tattattttt
    21301 aacatatttt ttattgttaa aaatcaattt ttttatgaaa ttaatttttt tatctttttt
    21361 ttatctatta aaatatatag atagaatcca ttaataattt aaaaaaatga ttttttttta
    21421 ataattagaa atgatttttt ataatttttc attatatttg taaatataaa aaatattatg
    21481 gcagaaaata gtaaaacatc taaaacaact aataaaaacg taccaaaaag tactaatatt
    21541 ctagagccaa tagagataat tggtggtaaa ataccaccaa gtgatttaga aacagaagaa
    21601 gtagtattag gtgcattatt aatagaacct aatgcagtag ccactgtaat agatatcttg
    21661 cagccagatg ttttttacaa agaagcacat caaaggatct tttctgctat tttatcttta
    21721 tatcctatag gtgaaccaat tgatataata actgtaacaa actatttaag gagaaatgga
    21781 gaattagact taataggggg gccagcttat atagcacaac tcactgacag agtaatatca
    21841 gctgctaata tagagtatca tgctcgtatt ttaatcgaaa aatatattca aagaaaattg
    21901 attcaaatat cgaatcaaat tattactgaa gcttatgatg ccacttcaga tgctttcgaa
    21961 accctcaaca atgcagaaaa tgcattattt caaatcaatg aacaaaatct acgccgttct
    22021 tttaaatcta taccatctgt attgcaattg gtaagagagc aaatagaaca tgcatataat
    22081 aatcaaggtg attatacggg agtgccaaca ggttttttcg aattagataa attaacggga
    22141 ggattacagc cttctgattt gataattata gctgctagac ctggaatggg caaaactgct
    22201 tttgctctat caatggcaca taatatggct gttgaatttg atatacctat tgcttttttc
    22261 tctttagaaa tgagcgcacc acaattagta atacgtttat tagctattga aactcaaata
    22321 tctagcgaaa gaatacgtaa aggtgaatta gatgaagcag aatgggaaac attaaataac
    22381 gctatgaata agctgtcaaa tactaagttg tttctagatg atacaccagc tctatcaata
    22441 tttgaattaa gagctaaggc acgtagacta aaacaagctt acaatgtaga agcaatcttt
    22501 gtagattatt tacaactaat gtcagctaaa atagatagaa atgctaatag agagcaagag
    22561 atagcttcca ttagtagatc attaaaagca ttagcaaagg aactaaatgt tcctgtcgtt
    22621 gcactttcgc aattaagtcg agaagtagaa aaacgtagcg gaaccaaaag acctcaatta
    22681 tcagacttac gcgaatctgg tgctatagaa caagatgctg atattgtact aggtatttat
    22741 agaccagaat attatctaaa agaagaaact gatgacgatg ataaaatttc tgaaataaaa
    22801 aataaagccg aagtaatagt tctaaaacat agaaatggac cattaggcac cattaatctt
    22861 aactttatta atcaatatgc atggtttaca gatgaagata tcagccccat aattactaaa
    22921 agacagattt ttgaatctaa aattaacgtc gaagtagatg aggatttttt gaatgaagac
    22981 aatgtgcctt tttaaatgtt ttatttaaat ttgtttaccc aataaaattt gaaaataact
    23041 tattacatta tagatctgtt atacattgca tatcgtaaaa tatgattttt atgtaacttt
    23101 ttttatgtaa tttcgttata ccattagagg aattttatga ctactaatga atataataaa
    23161 tctgtagata aatatggtga tagattatat gcttttgcat taaaagcatt aaaagatgac
    23221 aatatggcaa aagatatcgt aaaagagagt tttttgcgct tatgggaaaa tagagataaa
    23281 gtaaaaaact ctaaaatcaa accatattta ttcaccattg cttaccactt aatagtagat
    23341 gaaactcgtt ataaaaaaag atttgaatct ttgtatttta ctaataatga tgaggaaaat
    23401 gaatatgaaa taaaaaataa tataaaagat ttttttcatg ataactgcag ttatcatgaa
    23461 aaaattgaca atttcgacct aaaattacat ataaataatg ctataaatac attgccagat
    23521 atgcaaaaaa aaattttgaa actgcgtgat gaagacggat atagctataa agaaattgct
    23581 gattatttaa atattagtga aacaaaagta aaagtttacc ttcatagagg taggcaacaa
    23641 ataaaagaaa taatcgggaa aattgacaat ttattatgaa aaagaaaaaa tttaaaaaat
    23701 tattatatca aaatttgaat tctgataaaa gaaaagataa gagatatatc gatttatttt
    23761 atcaaatata taatgatagc aaaaattcta atttaaaact agatgaatct tttaaagaat
    23821 ctcttaaaat agatactaat gataatatca tcaaaaaaat cagctattct atgctaaaat
    23881 atgcagttgt gatgattgta gtgcttgcaa ttggcattat agcaggtata ggtatatcta
    23941 ataatttttt aacaaaacaa gaggcttttt tacaattccc taataattat tttgtgatag
    24001 ataatataca tctaaaacct caaacatcaa atgaattaat atcaaaaaat aaattatcaa
    24061 caacaatatt acctagagat tcaaataaaa caacaataaa taataatgac actacaaata
    24121 ataatttagc aaaaattgat aatttaaaaa ctataaaaac atctacacct ataatacagg
    24181 taaaaaccga agtagataca gcggaattaa ttgatacaaa aatattttat caaggtttag
    24241 aaaacatagc gagaataata gaaatggaag ataatgatct atatgaaata gaagaacaat
    24301 acaaaaaaat aaaaacaaaa gatcttttag ttgtttggca agatatcaaa aattttatca
    24361 gtgatggaca agtgcccttt aatgtctctg taactaatat atttaatcta ataaaaaatg
    24421 taaaaatcaa aaatcaaaaa tattgaaata tatttcataa tgaaaaaatt atttaatttt
    24481 gtaaaaaatt aagcccaggt ggcggaattg gtagacgcgc tactttgagg gggtagtgcc
    24541 tgcaagggcg tgcaagttcg agtcttgtcc tgggcatttt tttaattgcc cggatggcga
    24601 aattggtaga cgcgctagtt tcagggacta gtactcatat agagtgtgca ggttcgagtc
    24661 ctgttccggg cattcttcta aatgactaat tatatatgct tggaatatat tcacaatata
    24721 atgtagtatt ttagtaacaa aaattatttt ttaacgtata ataagtttca acctaaatct
    24781 tttaaataat attatgaaac ctaaaaaatt gatgcatttt aattttgatt taccagatga
    24841 attgatagct cgatacccta tagaagaaag agatgaatgt agactgatgg tagtacataa
    24901 aaaaacagga gaaatagagc acaggatatt taaagatatc attgaatatt ttgacgaagg
    24961 tgatgtgatg atagtaaata atacaaaagt ttttccttct agattgatgg ctataaaaga
    25021 acgaactggc gcacagatag aagtaatttt gcttagagaa ttaaatccag aattaaagtt
    25081 atgggatgtc acagtagaac ctgctagaaa aataagaatt ggtaacaaat tattttttgg
    25141 cgaaaatgct gaattgatag ctgaagtaat agataataca acttcgagag gtcgcgctat
    25201 tagatttcta acagaaatgc cacatgataa atttttaaca atattaaaaa gtcttggcga
    25261 gctcgctgta ccaataattt tagatcgccc catcgaaccc attgataatg aatattttca
    25321 aactgtattc gctaaggtag aaggttctgt cttacctcct ttcgcaggac ttcattttac
    25381 tccattatta atgaaacgta tggaagtaaa aggtattaat tttgccgaaa tcacactaca
    25441 tattggcaca ggagatttac gcaaaattga tgttcaagac ttagctagac ataaaactga
    25501 tgctgaagaa tttattataa ctgaagaagc ctgtcaaata gtcaatgaag ctaagaaaaa
    25561 caaaagaaaa gtatgcgctg taggtgattc tgttttaaaa gcaatagaat catctcatac
    25621 tattgacggt tttctgatgc ctgcacatag atggacttca ctttttatat atccacctta
    25681 tgaatttaat gttcctgatg cttatcttac taattttaac ttgccttata gtattgctct
    25741 aatgtctgca gctacttttg ccgattatga tctcattatg aatgcctatg agatagctgt
    25801 aaaagaaaaa tatcgtttcc atgtttatgg tgatgcaatg ttaataatag attaaatgaa
    25861 taataattat tcgattattg tggctgcagg tgctagtaca agaatgaata gccaaatacc
    25921 taaaccttat ataaaagtaa ataataaacc tttactctat tatagcttaa atgcctttat
    25981 caatgtgatg cctgcatgta atattgtagt agttattaat aataatcata aaaaatttta
    26041 taatgatttt attcatgaat tcaatgagtt tatagatcta acttatgtca caggcggtga
    26101 gcaaagattc gacagtgtaa aaaatgccct ttctattttg ccagataatg gctttgtagc
    26161 catacatgat gccgctagac cttttttacc tatatcattg ataaataatg gatttgcttt
    26221 ggtcaaagaa tatgactctg ctattccata tcatcaagta ccaaattctg taagacttaa
    26281 gcaaaataat aatacaatac ccctaccgag aaaaaatgta atgataatag atacaccaca
    26341 attttttaat atttctctat taaaaatcgc ttatgagcaa aaatttaata ctgattttac
    26401 tgacgatgct agtgtttggg aaaaatttaa tttaccactt tatttctttc aaagcactca
    26461 cctaaatatt aaggttactt acccagaaga tttaatatta atttcatcta ttttagaaaa
    26521 aaatgtgtaa ttttgtaaaa taaatttccc atgcttttat gcgtgggaat gttttttatt
    26581 atttataaat ttattcaaat ttttaatacc atggaaacgc caacatatct cacaaaagaa
    26641 ggtttcgaga aacttaaaaa taaattaaat aaattaatcc atgaagaaag acctcaaatt
    26701 gctcaaatga tagctgaagc tcgagaaaaa ggcgatataa ctgaaaatgc cgaatatgac
    26761 gccgctaagg atgctcaatc taaattagaa aacgaaatat cacagttaca actcctttta
    26821 gctaatgtaa aaataataga agaagatgaa atcgataaat cacaagtgag aatactcact
    26881 gtagttacaa ttaaaaactt gtctaataat caaatcatat cttataagtt agtccctgat
    26941 aaagaagctg atctgaagca aaaacactta ggtattaata gcccagtagc ccaagctctt
    27001 ctagggaaaa aaatcgatga tatagtagaa atcaatgtac cagcaggaat aatcaaatat
    27061 aaaatattaa aaatagaata attatgtcta gtatttttac caaaattgtt aatagagaaa
    27121 tcccagctta tatcatagct gaggacgata agtatcttgc tttcttagat atcaatcctt
    27181 tagccaaagg acatacactt gtgatcccta aaaaagaagt tgattatatt tttgatctag
    27241 atgacgaaac cttgacaggt ctttggcttt tcgctaaaag agtcgccaaa gctatagatc
    27301 aatatactaa ggatgtagct atacgcacag gagtagccgt agtaggtcta gatgtaccac
    27361 atgctcatat ccatttagtc ccaataagta atgtggcaga tatgaacttt gctaaaccta
    27421 aactaaaact cgatcccaaa gagtttgaaa gcatagctga aggaataagg agttttttaa
    27481 aaaaataaca actgacaaag atttatattt cttgataaaa attgacaaag aaaaaattaa
    27541 aaccaaaaag ttgaattaac ccccgacctt taggtcggaa aataataagt tactgacaac
    27601 atccctgttg ctacattaac ctgtgaaaca taaatgtata gttatctttc ataaaagaca
    27661 ataagataaa aataaacaaa tatacatttt gattatcata ttttattaaa tttaaaattt
    27721 ataattaatt ttgcgaaaaa aatggcaaaa gctattactc aagtattgag taaacatata
    27781 ataggaaaaa gtaaaactga ggtattagaa atgtttgatg ctattgctcc taattatgat
    27841 aagtttacat ctttattttc atttggtata gaccattact ggagagaaag tcttacttta
    27901 gaattgaaaa aatataagcc tcatattata ttggatttag cttgtggcac tggggcacaa
    27961 gctaaatcat tacttaaatt aaatcctcaa aagttgattt tattagatgc ctccgaatct
    28021 atgataaaaa tagccaacga aaaactaaat tacccaaata tagaatttta tcattcttat
    28081 gctgaatata tacctatagc tacctcatcc gtagatgctg taagcatatc ttttgggctt
    28141 agaaattttg aaaattctta tatggcttta ttatcatctt atagagtatt aaaaaataat
    28201 ggaattttaa ctattttaga atttggtaaa cctaataaag gttttgattt attatatgaa
    28261 atatatcaaa acactatcat gaatgctttt gcagacaata tattaaaata tcaaaatgct
    28321 tttgaatatt taaaaaatac ttcttctgaa ttcccttatg gaaaagatct atctgcctac
    28381 atagaaactc ttggtttcaa aactttaaaa ataagaaaaa tgaccttagg tattgcctat
    28441 ttatatattt ttaaaaaaaa atagataatt tgtaaatttc aataatatta ttaattttta
    28501 tgcaataatt taaaatatta ctattatttt tgtttataaa agattgaaag atgttaatag
    28561 aatttttaac taatacttta aaaaagattt tcacacaata caatatctca ccccatgagc
    28621 tcaaaatatc atctacagag ccaccacata caggtgattt cactttttac cttttcccat
    28681 tattaaaaaa aactaaacaa aatcccgaac aacttacaga gactatcacc aaaatactct
    28741 atgataacaa aataatcgaa aaacatgaat tgattaaagg ctttctaaat atacaattga
    28801 caaatgaatt tttattaaat ttttttcaaa caaaaagaga gaatttattt gaccttcctc
    28861 aaataggaac aggcaaaacc atattagttg aattttgctc tcccaatact aataaacctt
    28921 tacatttggg acatctacgc aatatttttt taggaacatc cattagtaat atattgcaac
    28981 agctagatta taatgtaata cgtataaatc tcatcaataa tagaggtata catatatgta
    29041 aaagtatgct agcatatcaa aaatggtttg gacatgaaac accagataat actaaaatga
    29101 agggagatca tctagtaggc aaatattatg tagccttcga aactgaattg aaaaaacaaa
    29161 taaaaaaatt agtagctatt ggtatatctg aagaggaagc catgaaaaat gcacctataa
    29221 tgaaagaggc ttatgaatta ctaagaaaat gggagtctgg tgatcctcaa gtgctagctc
    29281 tatgggaaaa attgaataat tgggtaataa gtggtttcga acaaactctt aaacgtttaa
    29341 aagtaaattt tgatcaaata gatctcgaga gtgaattata taaatatggc aaacaaatca
    29401 tcttaacagc ctatcaaaat ggattattag aacgagatga gactggtgct atttttataa
    29461 atttagaaaa tgaaggctta gacaaaaaaa tattgttacg agctgacggc acttctatgt
    29521 atataactca agatattggt atagcagtgt atcgttatca aaaatattgt ccagaccaaa
    29581 tgatttatgt agtaggtaat gaacaagaat accatttcaa agtattaaaa tctactctaa
    29641 aaaaacttaa ttatcaatgg tatagcatta ttacacatta tagctacggt atggtagaat
    29701 tgcctgaggg caagatgaaa tctcgagagg gtacagtagt agatgccgat gacctattag
    29761 atgagctaaa atatttagcc aaacaaacag cttgtgatag tggtaaatta aaaaatttag
    29821 acactaataa agcagaagag atctatgaac aaataggaca aggagctata agatatttta
    29881 ttttgaaggt agatagtaaa aaaaatattc tttatgatcc taaagcctct ctcgatttta
    29941 atggtaatac aggtccattt atccaataca cttatgctcg cattatgtct ctattgagaa
    30001 aaaaaggtat cgaactcgat tacgccttaa ctctgaaata tgaaaaaatt gacttaaatg
    30061 atttagaaaa gaaaattata ttagaattat tgaattatcc aaacataata ttagatgctg
    30121 ccaaatcatt aagacctgag ctcatagcta attatgctta tcgactggca gataaatata
    30181 acagttttta tcaaaatata ccagttttat ccgaagcaga tctaaataag caaaacatga
    30241 gattgaatat ttctgcatac actgctaaag taataaccca atctttacat ctattaggaa
    30301 tagaaacgcc agagaaaatg taaaaaaatc atagttatga tttttttact taatacgaat
    30361 tcgtatttac atattaattt tagaatttac ctaaaattaa tattattaga aatatttttt
    30421 aattcagatc gaaaaaaggc tttctgaaat taatcattgt tttactttac gtattttata
    30481 tcattatcaa ataattttta tttaatttta caaaaaaata attatggata atttcaatct
    30541 attagattat caggctctac ccaaagaaca aaaacgccga tttctagata atctatatca
    30601 atttttatta gaaaacaact acacagccgt agattatcag aagctaaaaa tacaaagcac
    30661 agcccccatc tgtcctagat gcgaaagcga aaatgtaata aaagctggag tacgcgacgg
    30721 caaacaagtg tacaaatgca aagattgcgg ccgacaatac cgcgaaacag ctagaacctt
    30781 cgtatatcgc atgcgaaaag ctgataaaat gctcgattac ctaaaatgca tgttagaagg
    30841 caaaagtcta agagcttgcg catcggaagt aggcataagt ctgagaacga gtttcaattg
    30901 gcgacacaag atactagcag cgatcaaatc attacagggt ggtatcagct tctcaggcat
    30961 tgtagaagct gatgagctgc taatgaaata ttcagaaaaa ggtcgcaaat acagaagccg
    31021 aaaagaatac gaaaaagcaa agaaaaagaa acatcccaaa gtagctgtgc tagtaatgac
    31081 agacagggaa ggcaatttac ttttcaaaca cgtaggacgc aaaaaagtaa ccaatagtca
    31141 gataagagaa gagctatcgc atagagtgac agatagcaac ttaatatgtg tgaagcaaaa
    31201 gaaggaattc aaaaaagctg tgaaagggac caaaagtaaa aaagttgttg taaacaaaac
    31261 gcagattaga cgcggtgtat acagtgtaca ggttgtcgat aagcatataa atgatttcaa
    31321 ggtttggttg cgacgattta gaggagtggc tactaaatac ttgcagagct atctgatgtg
    31381 gtttgtaatc acacataaat acttgacaag tgagatagaa tctgacattg gtaggctttt
    31441 aaatcttgca tcctcggata ggtgggcgtg ggatgagtat tggcggctgg taaatacaaa
    31501 gtatagataa actacttcaa gtaaaacaat gatttgtttc tggacccctt tcgattcctt
    31561 tcacaattac aataatttcc ccttctataa tttctattcc aattaaaata atcatagcca
    31621 ggaaatttag ctttgtaaaa attacgcttc ataaaggcga tttattgagc gaagctactt
    31681 aaaaatatcc tttgttttac ttgaagtagt ttatagcata attaaataat tagacgcggg
    31741 gtatacagtg tacaggttgt cgataagcat ataaatgatt tcaaggtttg gttgcgacga
    31801 tttagaggag tggctactaa atacttgcag a
//
LOCUS       DAOPRN010000020        30037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1206_length_30037_cov_99.853946, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000020 DAOPRN010000000
VERSION     DAOPRN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30037
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1206_length_30037_cov_99.853946"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..2674)
                     /locus_tag="PKI65_03755"
     CDS             complement(140..2674)
                     /locus_tag="PKI65_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOB77590.1"
                     /translation="MNKLKKLKQIILFLPLLLLMASCSVPRTYPDKQFIVKNEVNIVY
                     DPLDTIQAIKKNDFNPYIKLQPNRKTLFIFRFPSSVYAFADKHLKDKTEIKKQKSEER
                     IAKKRKKGKSINYDKEQRKIEKGLRNYLLNEVGEPPTFYDKYLSNISMEQMQLYAKTK
                     GYFYTQVKDSLIPIGQKKYKLYYEINLGRPYMISSIDWTFLDTTLEENILSAIFKKPL
                     RYHSLLTYSQINWHYLLNSFKLSVLSYIPNLPLKPGIIYDEKILDDARNKLATHLRNS
                     GFYNYSKSYIQYEADTTIGNYQMHLNTLLFPYQKQIDNNIVTLPFQKYKISKVYINLA
                     YNPLSKNELSCDTVLYTSKLSIDTFYFVYPKGFYFKPQVIANKIIVKPNQFYSESKGF
                     RTEALLLDIPIFSNALVEYKIDTSKEGNNLIANVNISRYDLQNWRFDLETTTSSGVYG
                     LNTVISYINRSIFNGGEVLVFQARGGLEVENLFSGHDIFRAYYYGADLSLSFPKLLVP
                     FVKIENTSVNTKPSTQVQLSYLFQKRIQYTRTLIGANYSLQFNETRYKFHQIHVPYIN
                     LIKINPDSSYKEVIENLPSRLRYGYEDYFSFGVRYSYTLSNKKTYPTHMPYLYFRFNA
                     ETSGEIFWLYHQIFTKNANTDQTQRVWNIPYAQFLRMDGDFRVYFPGKKNVLSVVRAF
                     VGIGYPYSNSNYMPFERTYSVSGPSEIRAWPYRTIGPGEYRSTTAKDSIIERGSEIAG
                     IINLETRFKVYNFINLALFIDMGNGWSVRNNALYPGGKFEWNKFYKQLYLGCGLGLRL
                     DFNIIAIRFDFGFPIHDPSLPPDEKWYSFNKSIKNMNFNFGIGYPF"
     gene            complement(2667..3908)
                     /locus_tag="PKI65_03760"
     CDS             complement(2667..3908)
                     /locus_tag="PKI65_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOB77591.1"
                     /translation="MKKLTLLYENIKIAITAIFGHKTRAIITILIIAFGIMALVAILS
                     SIDAIKMSFTESFTRMGGHSFTIYELPNQNSESKKERIERKPISYHEAITFKHKFDYP
                     AIVSISTNLPQLFTIRSQYKETNPNIQITAVDENYLKTAGLTLENGRDFLSQDIEATK
                     QYVILGKELSNNLFPYDIEATNKEINIHGIKFTVIGVLEEKGSTFGSNIDRTCLIPIS
                     TAKKYFIKSQTSHRINVMVYDVNKLDEAIDAAINLFRQIRQLEISQKNNFATLKSDFI
                     VDVLMQNLKYLNLAAIILGMITLLGSTIGLMNVLLVSVTERTQEIGIRKALGAKSKDI
                     KTQFFIESIILGQLGGLLGIILGVLIGNVVAKLLKIGLVLPWGWMILSIVLCLCVSIL
                     AGLLPAARAARLDPIESLRYE"
     gene            complement(3905..4387)
                     /locus_tag="PKI65_03765"
     CDS             complement(3905..4387)
                     /locus_tag="PKI65_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77592.1"
                     /translation="MIKIISFLFKNFYLLIIGATKFMFTTSTAKISGLNFYEAFFVTA
                     FGGIMGYFFFYYLFDGVIKLYNNIHSHYTKPSKPKKPPKTVPYKKRKRYVKWVRHYGY
                     RGLIIFTPCFISIPLGAFLLRRYFPNKKNKDLLVSISIILWATLYTFVLYHSEMINAL
                     "
     gene            complement(4440..5090)
                     /gene="upp"
                     /locus_tag="PKI65_03770"
     CDS             complement(4440..5090)
                     /gene="upp"
                     /locus_tag="PKI65_03770"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOB77593.1"
                     /translation="MIHNLSNSDTILSQYLMEMRDKVIQKDPLRFRNNMMRVGEIMAY
                     EISKQLPFKKIKVETPLGIAVSSVLAEQPVLATILRAGIPFYQGFLNFFDKAPSAFVT
                     AYRNHINGNEFNINVEYVSTPPIDNKILILCDPMLATGNSIILSYKKLLTHGMPKEVH
                     IASIISARAGIDDVSQNIDHPKLNIWTAAIDEELDENYYIVPGLGDAGDLAFGKKE"
     gene            5173..5739
                     /locus_tag="PKI65_03775"
     CDS             5173..5739
                     /locus_tag="PKI65_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77594.1"
                     /translation="MKKSKILSVTIAFIILTGCSSVQLSERYTYLNKVPVNQTVETIK
                     KPIKQDKYLVDNELANHVDVDIENNDIDAIYDKHSDNLAYDNVDNLKPLSFVQKKQVV
                     PVKAKNIFPILPSFKANHFKQQLNEINYESIADTDSLTKVLIGLLILALILIVLDLIL
                     PGDVFAIISTIILVLILVYAIYFLFKLL"
     gene            5779..7332
                     /locus_tag="PKI65_03780"
     CDS             5779..7332
                     /locus_tag="PKI65_03780"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOB77595.1"
                     /translation="MSQVKILWVDDEIDYLKPHIKFLEDRGYVVYTATNSSDALEMLQ
                     KENFDILFLDENMPGMRGMTMLEHARSKNITIPVVMITKHEEEHLMEEALAHRITDFL
                     IKPVNPSQILLTLKKVLDKKDIQKKKTIDDFQQDYRELIMNLSPSMTIEEWKEVYKKI
                     VDYEIRFQSLNETIFNNTILSLKEEANEVFAKYFTHYYPDWLSEAETTAITFSHTLLK
                     KHLFPHLDNNSRVFFILIDNLRYDQWVMFKPIFEEYFQIINEDLYCSILPTTTEYARN
                     SLFSGLLPNGIAQLYPDFLEGDEDISSKNQFEDELFMNYLKRYGKNLKVDFFKILHPP
                     MAQRYIKSINELKDNNVNVLVYNFIDTFAHAKTEVELVKELAKDEVSYRAVTYNWFIH
                     SILIDVIKWAAENHFSIFLTTDHGSVQIKNPIKIIGDRETNTNLRFKTGKNLSTDEKK
                     VYWVRKPNQIGLPIQNVSDTYVFALKYDFFVYPNNYQYYSRYFADSFQHGGISLEEIL
                     VPYVFMTPK"
     gene            complement(7440..9833)
                     /locus_tag="PKI65_03785"
     CDS             complement(7440..9833)
                     /locus_tag="PKI65_03785"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOB77596.1"
                     /translation="MKKQNILFVIMLMLSFSLNSQIIITGKVIDQETQSPLPWANIKI
                     DGTYLGTVSGNDGNFTLNVKSLPVTLIISYMGYETDTVVVKEQQKNLIALKRKSILTD
                     EIVVSAIRQEDKSPKTYTNVTIEKIQSLKAGQDLPLILNFTPSVVATSDAGNGIGYTD
                     IRIRGTDITRINVTINGVPYNDPESQGVFWVDIPDIASSSDNIQIQRGVGTSSFGAAS
                     FGASINILTGVLKADPFVELQALGGSFNTFGASAKFGTGLIKDNWYLEGRLSHQYSDG
                     YIDRAFSELNSAYLSGGYYGKNTIIKGLFMAGYEKTYQAWGGVPKEYLNDAKLRRYNP
                     YTYENETDNYWQYHYHLNITQKLNEKNTFNATLFYINGYGFYEQYKKDKKLSNYNLEP
                     VILIDTTRNDTITIKSMDLVQRKFLENDFYGIIMSHIYNNNKNLRIRTGLNYYEYDGW
                     HYGRIIWMQYAHNVPINHEWYRNRSLKKEFSIFTKFDYDINEFLTLYADIQYRYVDFT
                     IKGTDDAFISDTLRKFYNFWNPKLGLNYNISQNDELYLLTGISHREPNRDNLMLTDVD
                     SLRPVHETLYDLELGYTRKFKNGLININAFYMYYDNQLVLTGKINDVGDPIMQNVPES
                     YRTGVELIWNFDFTKWLSWDANFTFSENKIKKITMYIDDYELWPSQRSLVLKNTPISF
                     SPSIVASSILTFKPLPALSISLLSKYVSKQYIDNTGDESCTIDPFMVHNIRIDYTIKG
                     KKFDKINLFVTFNNIFNEFYETNAWVYRYYDGGVEYKEFGYFPQATFNVIGGLTFRW"
     regulatory      complement(9882..9984)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10210..10728
                     /locus_tag="PKI65_03790"
     CDS             10210..10728
                     /locus_tag="PKI65_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77597.1"
                     /translation="MDSINFVYNILPEALIDSAYKQSYYPVISYDSLRNILNKTCNDR
                     DLKYLYSIIPYREDTFLFIISSYTHEDILNDMISKYLMPYYHYPDVLNTLSADNPINY
                     TSYSDEYGSFYSAFHYFQTSKGNPYIIGADYSLDKIISLNWKFIFIILGVTMIFIFIY
                     IYHKNLKYNNGL"
     gene            10917..13613
                     /locus_tag="PKI65_03795"
     CDS             10917..13613
                     /locus_tag="PKI65_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77598.1"
                     /translation="MKRFIVYLFLFNVISFAFAQGNISVKIEVNDLKEHNAIADAQVS
                     LGEFIKQTDDKGIATFSLLPGIYGITVNAAGYQSYTDIIDLLSAKDTTIIVQLARGLS
                     LSIQQELALEQELEIINDDFDRGASINPLLLSSRDPFLNLASYNLSSGSFSPRGIERG
                     GYEAYINEVLLYNPELDKINYTDFSGLNDAVRYSDDSYGLEPLYLSFGDIMGVSNKLA
                     TPSRMRLGHQLSYGFSNQSWQHRLMYTYNSGIMKNGWGFAVSYSHRYAYEAYIPGTWL
                     QADAFFFSAERKFSDRHSLSATIFASPQKRAMATPTVKEVQELVDNPYYNPNWGVQNG
                     EKRNARVRSIFKPYLIIKDDYKFNDNFTWSNAFGFTYGIYGTTRLNWYNAPDPRPDYY
                     RYLPSYQTDPYIAEIVTQQWKNDVSVSQINWNKLYQINYLANLENKSARYIVEDIHND
                     PLYFYYGSNIKYTFNPSSIIYGGLQAYHYETHNFKKIDDLLGANYYLDIDKYAERDFP
                     DNPYMLYNNLDEPDKPLYEGDVFGYNYRLNTNEISLWTQYKQTLPRIDYYVAGKIGFS
                     NKYRNGLYRSGRFPDNSKGKSDQVNDFIGGLKTGAIFKISGKHYIKANIAGFTKPQSI
                     IDIFLSPYNSNRLNNDITTQKVLSGDIGYHFNFKFLRGRITAYQTYILDHMDMRGFYH
                     DEYQTYVYMSLSNMDELYQGIELGLEANIMPDLYISAAGNLGNYRYISRFKANVSFDN
                     LSRPDTSYTVYSKNFYIPNVPQRVGSIAINYKPNYWWFTLTFNYFDEMYMDFNPERRT
                     QFALNNLGPEDPLIKDIINQKELYSGFTLDLSISKTWMIKSQYRLGVNLFINNILDNQ
                     KLALFAFEQSRFDFDTKNIDKFPPKYMYAYGRTYMLLIKLSF"
     gene            13634..14914
                     /locus_tag="PKI65_03800"
     CDS             13634..14914
                     /locus_tag="PKI65_03800"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOB77599.1"
                     /translation="MKFIKNFIVIASMILLFAGCVDDEFDVPPIDIPHVDFEANTTIA
                     ELKAMYSGGLDSIDTNIIITGVVVANDESGNFYKQLEIQDNTGGIEVRLDRYDLYTQF
                     KVGQRVYIKCQGLFLGNYGGLIQLGYKYQGGIGRIPDAIIDQHVFRDSLPGTPPPPIK
                     TTIPGLNTNYLSMLIQIDSVHFGEVGAKFAESDAITNRTILDREGHTLILRTSNYANF
                     ASELLPPGEGSIRGILSIFNGTYQLYIRDLNDLVNWNEDATLPFLETFSSNPLANGTW
                     LTYSAASNKNWTYNSSNGNPGGCMEMNGYGADVASDDWLVSPEFNLTSATAASIKFES
                     WTQYSDNGITQPLQVYILENYASGDPSQATATNISDRCIMPEANSKVWISSGTVNIFD
                     FIGKKIRVAFHYKSSGTGSSTSTLWRIDNVALNY"
     gene            15434..18712
                     /locus_tag="PKI65_03805"
     CDS             15434..18712
                     /locus_tag="PKI65_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77600.1"
                     /translation="MYRLKTNFFVILLIIYSTIANAQFYSGSQLNFGKNRVQYRYERI
                     WSVMKFDYYDIYFYQKGYPIAVNTARYADSIIHNYENKLNYHLKSKLQFIVFNSLSDL
                     KSSNIGIDDELLYNSAGQTIIERNKIFLYYNGSYVDLFKQIREGVARVMVERFLYGET
                     FGANFKNQTLDAIPSWFIDGLVSYLAEPWSTEMDNLLRVNILDKKFKRFNQLEGEQAR
                     VAGHSIWRYIVDIYGENVISNVLYMTRLSRSVSDGIIYSLGITYKNFINAWYNYYEKL
                     YKNEIEQWEAWENMYTLPISKKDNPYKVALSSDGQYAAIVADRMNQKRVYLVSIQNPK
                     EKKLILKLGPKVEEAFDDSYPLIAFHPYAPLLSCYYENKGQRMLMLYNVDEDTKEYIK
                     IESLDKITSISYSPDGMNLAVSAINEGQSDIYLFSFAGKNFTPITKDHYDDYGPVFIN
                     RNQIAFLSNRTKDTLEYVRKTYKYDYFSWRNTPGNYDVFIYDNRKPDILIQGTKTSMV
                     NENEIFAHTKDDFLFLSDSNGVNNLFIAKIDSSLLIVDTVMQYKYFADYKPITNVNNS
                     ILTASIHDNKALIITKDKNNKNKISQVSLLFTPKDSLPLSYYKKQQLKKLFAKSNINK
                     KQIETKTIDAHKQNYQIIGIDDKNDTLIDIYNYKLEQDTITIDKIEKKESKKHKTKLL
                     TTIPHTLDDFALPKVETYEVAYYIDEVTTQFDFSYLSSTYQPYTGYGPIFIQPGMNAL
                     FLFNLTDLLEDHRIILGTHLSGSLDENEYIVSYEDLSRRADKQIVFHRMSYYSNEEEF
                     LKKNTIHDIHFRWKYPFNQVSALRIDIFAKYDKIAYKSIDNVSLMQPDEHELWAGGVI
                     DFTYDNTRNPLINIYYGTRGKVFFEYYQGVLNHKLNLFNVGFDFRHYTKIYRSLIWAN
                     RIAGGTSFGSTRLIYILGGVDNWLFPRYMYQPVLDPSMNFSFQTLATNLRGMPQNIRN
                     GNTFFVINSEIRFPIFRFLFNRPLKSKFLNTFQLNFFGDIGSAWLGLNPYADINIYVP
                     EYYYKKPITVVINNARNPLVGGIGFGARADLLGYFMRLDYAWGIEERGILSPRLIFSI
                     TKDF"
     gene            19118..20059
                     /locus_tag="PKI65_03810"
     CDS             19118..20059
                     /locus_tag="PKI65_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOB77601.1"
                     /translation="MKAGRLGFGEGVLDAGKKYDNLVVLGADITASVGLNLFKEHFPS
                     RFFSMGIAEQNIAASAAGMALSGLIPVFSTYGVFAAFRCADQIRVSICYNNAKVIIGG
                     AHAGVSVGPDGATHQALEDIAVMRALPNMTVLSPCDANQVKNAVLAAIEQIDGPVYIR
                     FGREPLPNFSPEIAHLQIGKAEILNDGTDIAIIATGSLVWESIEAAKILYNEYKIKAM
                     VINIHTIKPIDKNTLIDAAQKCNIMIVAEEHQKFGGLFSAVSEVCSEYYPVHIKSIAM
                     NDRFGESGKADELMKYYGFTATNIVNNAIEMLNKYKK"
     gene            20056..20781
                     /locus_tag="PKI65_03815"
     CDS             20056..20781
                     /locus_tag="PKI65_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOB77602.1"
                     /translation="MKRFLIIYFIFFVITNLKGQMDEKYLLGKFDPADDSNFVLVDKK
                     YTSDARDIYLRKEVYEAFIQMHEDAKKDSIILLIVSGTRTYEYQKKLWEDKWFGYTFV
                     NNKDLYLAVPDDLERTKEILKYTAMPGTSRHHWGTDIDINSVEIEYFQTDYGKLVYNW
                     LQNNAEKYGFCEPYTDFDSMRPYGYEPEPWHWSYYPLAKLILEDYTKTIKFYMMIDFA
                     GFEFFNKLEIIDKYVLGINPKCK"
     gene            20832..21905
                     /locus_tag="PKI65_03820"
     CDS             20832..21905
                     /locus_tag="PKI65_03820"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77603.1"
                     /translation="MCTIHPKCGACLYSTATYNNIWKTKINTAIKNLKPFVSNNVPII
                     MHPKDVLHYRKKILLHVKSAIIPPKIGLIQKSDIVNIDLCPLQPEAINKKIDIIKYSL
                     PQYDKFPLQYISFNNETVILVLKDHHSHLARYLDTTKAIFSNVDFPYLYLHLNPSTGN
                     KIFLKSYIYPVYGDLFLKDADGYWYHPLVFQQQIHSLHIESLQIAIDFLKGIDNFLDL
                     YCGIGIASSIAIKYFKNIYGIEHLAISIECAKKNANKARFFVGQVDNRMRTLVKKEPD
                     FFENYVMYTNPPRSGMGKQCIKEILKSPPSKIAYLSCNTKSLKNDLMLFNENYNINAI
                     HFFDFFPYTNHIETLVLMSKECR"
     gene            complement(21955..22512)
                     /locus_tag="PKI65_03825"
     CDS             complement(21955..22512)
                     /locus_tag="PKI65_03825"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOB77604.1"
                     /translation="MDNLKIKELKDKKTARKYIKQLINNINPDSREKKASQVWDRVSL
                     HPRFRNAKVVMTYWSFGDELPSHDFVLNWSFQKIILLPRIDGDNIVPVRFMGMEYMSI
                     ANKYGIIEPVGDPWQDYDKIDLVLVPALAYDHNGHRLGRGKGYYDRFLKKLNAYTIGI
                     AFEEQILDYIPYDDNDVKIDEVIFY"
     gene            complement(22515..24467)
                     /gene="dnaG"
                     /locus_tag="PKI65_03830"
     CDS             complement(22515..24467)
                     /gene="dnaG"
                     /locus_tag="PKI65_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOB77605.1"
                     /translation="MIKRSTIDFILDNVPIDEVVKEYVTLHKRGNNLFGLCPFHNEKT
                     PSFSVAPDKGIFKCFGCGESGNVITFLMKIEQLSYPEAIRTIAKKYQIPVDEDGSELD
                     EEKDEKEKIFILNQIALEWFKAQLWSSEEGVDIALSYLHHRGISDYTIQKFNLGYGLN
                     QRDAFRNHALKKGFTDEDLINAGLIIPDSLADRFRGRLIFPIFNVGGRVIAFGGRAIG
                     NNKNIAKYVNSPETLIYTKSNIVYNLNNAKKNISQQDLCYITEGYMDVISLVQAGIDN
                     VVASAGTSLTQVQIKTIHRFTNNVCLMYDADSAGIKASFRAIDMLLEEGLNVYLISFP
                     EGEDPDSFTHKVSTTELIDYLKTNKENFINFKSHHLIGDKTNDPIKKADAIRNILFSL
                     SHISDPLIQAEYLVYISKNFDIDEFTLREEFQRILRDRLKKQLQNTYNYNPITDKPKK
                     KLKNQKNLSSINNKINQQEKRILYILIHFGNRILTFIEKDEFNFDTQIDIPTWKYINQ
                     ELKLDKIEFAFYKDLYELIIDQYQTFNNFNINNIISDLNPEEQDLMYDLLLDTFKLSE
                     NWEKKLKIEVNTIENNDLLYYYEVECSILEYKALILDKLKNETREKIKKTNNQEEQEK
                     LLEEFAILKHLENQILKKISSRNLIR"
     gene            complement(24609..26069)
                     /locus_tag="PKI65_03835"
     CDS             complement(24609..26069)
                     /locus_tag="PKI65_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOB77606.1"
                     /translation="MIIFETISSKLENYEVIKLVQQFCKENNNIALVVGGAIRDSLLG
                     LEIEDIDIVIQGDWQKLAEFICEKLQINNLVFFKRFLAAHFVYKGIEWEITSARKESY
                     RQDSRNPDVEPADLEDDFLRRDFTINAIGIELWPQFGKVWDPFNGFDDLHNRIIRTPN
                     DANITFSDDPLRMLRAIRFASKLNFDIEASTWEGIKANKERISIVAPERISEELNKML
                     LLPIPSIAFNLLNDSGLLDYILPELTALKTIDTELDFSHKDNFKHTLQVLDNVCQQLE
                     NIPDHPNNLWLRWAALLHDIGKAKTKRYDEKTGFSFHNHEIVGAKMVPIIFKRLHLPL
                     NEKMDYVVKLILLHLRPIALVDDNVTDSAIRRLMVDAGEDLDDLMILCECDITSKNIR
                     KVKKFTDNLKNLKKKFDEIEEKDRLRNFQPPISGEVIMQTFDLKPSKLVGDIKTAIRE
                     AIIDGEIPNEYDAAYKFMLEYGKKLGLKPKDINFNQ"
     gene            complement(26187..27935)
                     /locus_tag="PKI65_03840"
     CDS             complement(26187..27935)
                     /locus_tag="PKI65_03840"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOB77607.1"
                     /translation="MSNITLYINNLLSKIIKTPTKDQQVTFINLLSAFLKPTRKDNHI
                     FMLTGSAGTGKTTVVEALIKTIPIINESIENNILMPIKLPKQPISVYLTATTGKAARM
                     LENRCQYSACTIHKLIYTLDDNKSEVSRSRLRWYYKIADPLTDPYSVLFIDESSMISS
                     LNQEDDNVIFGSGNLIDDILTFGTNAKIVFVGDPYQLPPVNEHKTLALDPELFEMKYF
                     FPVQYFELREPVRFSKYSGIYQIAYQIRDTLTSGNQSNINIEENGNVTLYDSVDEIIN
                     HHVDILKNKPTLPTTMIAYTNNVVSTLNKKIRSKLLKNTKKLLNNNEYVMVIKNNYLY
                     DLMNGEIVQIINMESECKRIEHLRFRKVKLKYGSDDNDVMKCMVLENTLEPNFNYTFQ
                     DELLILSYIFEKIQKQNPDLLDNKRSLMFRLQNDPYLNALRLKYAYAITCHKAQGGEW
                     EEVSIVTESMLYHSKQPSDFVLRWLYTAITRAKSNVNLLRRPARPADFIDELKFDVHY
                     KMGKFIPRYYTGYIYIKDFKIWTARSGVKYVSLDSNEILLIQKNLIECDIPDNLPCYA
                     FCKNGRIIRWQKDLDE"
     gene            complement(28130..29701)
                     /gene="nadB"
                     /locus_tag="PKI65_03845"
     CDS             complement(28130..29701)
                     /gene="nadB"
                     /locus_tag="PKI65_03845"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOB77608.1"
                     /translation="MHIFDFLVIGSGAAGLNFALKASKIGSVGIITKEELHNTSTYHA
                     QGGIVGVMYPPDNFDKHIQDTLEAGAYLNDREVVEITSKESPERIKELIELGVRFDKK
                     VTGEYDLAKEGGHSEPRIFHFKDKTGYEIERVLSNKVLENPNIDVFEHYFAVELITQH
                     HMGIKVYEAMTDIECYGAYVLTPEGKVERFLSKFTILATGGSGNVYLTTTNPTVATGD
                     GVAMAYRAKAQIMDMEFFQFHPTALYYPKERPAFLITEALRGYGAILKDIKGKEFMHK
                     YDSRKSLAPRDIVARAIDNEMKISGSEHVYLDCRHIKKDELLEHFPTIYAKCLSIGID
                     MTKEMIPVVPAAHYQVGGVVVDHYARTRINRLYACGEVSRTGLHGANRLGSNSLLEAL
                     VFSHRAALDVEKNLKNYSVNTNIPEWNDEGVKLNEELVLITQEVKELNQTMTAYVGIV
                     RSNLRLNRAIKRLQMLHEETEELYKKSIINRQICELRNMILVGYLITTMALARKESVG
                     LHYNIDYPFKNNKKQ"
BASE COUNT        10250 a         4393 c         4403 g        10991 t
ORIGIN      
        1 cttgatcttt taatatagtt ttcataatta taaattttgg taaaattaac aattatttct
       61 atataaatcc aatatgatat ttaacaattg caattttacg atacgtccca taaatccaac
      121 catccccaac tatattaaat tagaatggat aaccaattcc aaagttaaaa ttcatattct
      181 ttattgattt attaaatgaa taccattttt cgtctggtgg aagtgatgga tcatggattg
      241 gaaaaccaaa atcaaagcgt attgctataa tattaaaatc taatctaagc cctaacccac
      301 atcctagata tagctgttta taaaatttat tccattcaaa tttgccacct ggatacaaag
      361 cattatttcg tacagaccat ccattaccca tatctataaa taaagctaaa tttatgaaat
      421 tatatacttt aaatctagtt tccagattta ttattcctgc tatttcagaa ccacgttcta
      481 tgatagaatc ttttgcagtt gtagacctgt attcaccggg tcctatagtt ctatatggcc
      541 atgctcgtat ttctgaaggt cctgataccg aataagttcg ttcgaatggc atgtaattac
      601 tattgctata tgggtatcct atacctacaa aagctctgac tactgataga acattctttt
      661 tgccaggaaa atatacccta aaatccccat ccattcttag aaattgtgca taaggaatgt
      721 tccaaactct ttgggtttgg tctgtatttg catttttggt gaatatctgg tgatataacc
      781 aaaatatttc tccacttgtt tctgcattaa atctaaaata taagtatggc atgtgtgttg
      841 gatacgtttt tttattagat agagtataac tatatcttac accaaaggaa aaataatctt
      901 cgtatccata gcgtagccta ctaggtaaat tttctattac ttctttatat gagctatcgg
      961 gatttatttt aattaaattt atataaggga catgtatttg atgaaattta tatcgtgttt
     1021 cgttaaattg taaactataa ttggctccta ttagtgtgcg agtgtattgt atacgttttt
     1081 gaaataagta tgataattgt acttgagtgc ttggtttagt atttacagat gtattttcta
     1141 tttttacaaa aggtaccaga agtttgggaa atgacaaaga taaatctgca ccataataat
     1201 aggctctgaa tatatcatga ccgctaaata aattttctac ttccaaacca ccccgagctt
     1261 gaaagactaa tacctcacct ccattaaata tggacctatt aatatatgat attactgtgt
     1321 ttaatccata tacaccacta gatgtagttg tttctaaatc aaatctccaa ttttgcagat
     1381 cgtatctact tatatttaca tttgctatca gattatttcc ttctttacta gtgtctatct
     1441 tatattctac taatgcatta ctaaatatag gaatgtctaa tagtaaagct tcagtcctaa
     1501 aacctttaga ttcagaataa aactggtttg gtttaacaat tattttgtta gctattactt
     1561 ggggtttgaa ataaaaacct ttaggataaa caaaataaaa tgtatcaatg gataacttag
     1621 atgtatataa tactgtatca caggatagtt catttttaga taaaggatta taggccaaat
     1681 ttatatatac cttacttatt ttatattttt ggaatggtaa agttacaata ttattatcta
     1741 tctgtttttg atatggaaat agcaaagtat ttagatgcat ttgataatta cctattgtgg
     1801 tatcagcttc atattgaata tatgatttag aataattata aaaaccgcta tttcgtaagt
     1861 gggtagctag cttatttctt gcatcatcta atattttttc atcatagata atgcccggtt
     1921 tcaatggtaa atttggaata taacttaaaa ctgatagttt aaatgaattt aatagataat
     1981 gccaattgat ttgagaatat gttaataggc tgtgatatct tagtggtttt ttaaaaattg
     2041 ctgataaaat attctcttct aatgtagtat ctaaaaaggt ccaatctata gatgatatca
     2101 tatagggtct acccaaattt atttcataat ataatttata ttttttttgc ccaataggta
     2161 ttaagctgtc ttttacttga gtataaaaat acccttttgt tttagcatat agttgcatct
     2221 gctccataga aatattagat aagtatttat cataaaaagt aggaggttca cctacctcat
     2281 ttaaaagata attacgcaat cctttttcta tcttacgttg ttctttatca taattaatgg
     2341 attttccctt tttgcgtttt ttagctattc gttcttctga cttttgtttt tttatctctg
     2401 ttttgtcttt caaatgttta tctgcaaatg catatactga actagggaat ctaaatataa
     2461 acaaggtttt tctgtttggc tgcagcttta tatatggatt aaaatcattt tttttgattg
     2521 cttgtatagt atctaaaggg tcataaacaa tgttaacttc attttttact ataaattgtt
     2581 tatcaggata ggtcctaggt acactacaag aagccatcaa aagcagtaga ggtaaaaata
     2641 aaataatttg ttttaacttt tttaatttat tcataacgca gtgattctat tggatctaag
     2701 cgagcagctc tcgcagctgg taaaagtccg gctaaaatgc ttacgcacaa acacaatact
     2761 atactcaata tcatccatcc ccatggtaat actaacccta tttttagcaa tttagctact
     2821 acattgccaa taagcacacc taatattatt cctaatagac caccaagctg tcccaggatg
     2881 atactttcta tgaaaaattg agtttttata tctttacttt tagcaccgag tgcttttctg
     2941 attcctatct cttgagtgcg ttctgtaacg ctaactaaca atacattcat taatcctata
     3001 gtagagccta aaagagttat catgcctaat attatagctg ctaggttaag atattttagg
     3061 ttttgcatca atacatctac tataaaatcg ctcttaagtg tagcaaaatt atttttttga
     3121 cttatctcta attgccttat ctgtctgaat agatttatag ctgcatcaat agcttcatcg
     3181 agtttattta catcatatac cattacatta atgcgatggc ttgtttgtga tttgataaaa
     3241 tattttttgg cagtagagat gggtattaga cacgttctat ctatattact accaaaagtg
     3301 gagcctttct cttctaatac acctatcact gtaaatttaa ttccatgtat atttatttct
     3361 ttgttagtag cttctatgtc gtaaggaaac aaattatttg aaagctcttt gcctaaaatg
     3421 acatattgtt ttgtggcttc tatatcttga gataaaaaat ctcggccatt ttctaatgtt
     3481 aatcctgctg tctttaaata attttcatcc acagcggtta tttgtatatt aggattagtc
     3541 tctttatatt gtgatctaat agtaaataat tgtggtaaat tagtagaaat agaaactatg
     3601 gcagggtaat caaatttatg tttaaatgtt attgcttcat gatatgatat tggttttctt
     3661 tctattctct cttttttaga ttccgaattt tgatttggta attcatatat tgtgaaactg
     3721 tgcccgccca tacgggtaaa gctctcagta aatgacattt taatagcatc tatcgacgat
     3781 aaaatagcta ctaatgccat tataccaaat gctataatta gtattgtaat gatagctcta
     3841 gttttatgcc caaaaatagc agtaatagct atttttatat tttcatacaa tagagttaat
     3901 tttttcataa tgcattaatc atttcactgt gatacaatac gaatgtatac aaggtcgccc
     3961 atagaatgat ggaaatagat actaataaat ctttattctt tttattagga aaatatcgtc
     4021 ttagtaaaaa agctcctaat ggtattgaaa taaaacaagg tgtgaaaatt atcaaacctc
     4081 tgtatccata gtgtctaacc cattttacat aacgtttcct ttttttatat gggacagttt
     4141 taggtggttt ctttggtttt gatggctttg tataatgact gtgaatatta ttataaagtt
     4201 ttattacacc atcaaataga taataaaaga agaaataacc cataatacct ccaaaagcag
     4261 ttacaaaaaa tgcttcataa aaatttaatc cagagatctt agcagtacta gtagtaaaca
     4321 taaatttagt agctcctatg ataagtaaat aaaaattttt aaataaaaag cttataattt
     4381 ttatcataaa aaatcaattt aaagcactaa attagtattt tttatttaca aatttagatt
     4441 tattcttttt taccaaaagc taagtcgcct gcatcgccca atcctggaac tatataataa
     4501 ttttcgtcta attcttcatc tattgcagct gtccaaatat tcaatttagg atgatcaata
     4561 ttttgtgaaa catcatctat accagcccta gcagatatta tagaagctat atggacttct
     4621 tttggcatac catgagttaa taatttttta taacttaaaa ttatcgaatt gcctgtagct
     4681 aacataggat cacataaaat caatatttta ttatcaatag gtggtgtact cacatattct
     4741 acatttatat taaattcatt gccattaata tgatttctgt atgcagtgac aaatgcactt
     4801 ggtgctttat cgaaaaaatt taaaaatcct tgataaaaag gtatgccagc tctaagaata
     4861 gtagctaaaa ctggttgttc tgctaataca gatgatactg ctataccgag tggtgtttct
     4921 acttttattt ttttaaaagg aagttgttta cttatctcat aagccattat ttcgcctact
     4981 ctcatcatat tatttctaaa tcttaatgga tctttttgta ttactttatc tctcatttcc
     5041 attaaatatt gactcaaaat ggtgtcagag ttacttaaat tatgtatcat attgaaattt
     5101 tagacgaaat tacaaatttg ataataaata atatgaaata tttttaaaat attttttaat
     5161 tttgtaaaaa aaatgaaaaa gtcaaaaatt ttatcagtta ctattgcttt tatcatttta
     5221 acaggttgta gttctgtgca attaagtgag aggtatactt atctcaataa agtaccagta
     5281 aatcaaacgg tagaaactat aaaaaaacct attaagcaag ataaatattt agtagataat
     5341 gaactcgcaa atcatgtaga tgtagatata gaaaataatg atatcgatgc tatctatgat
     5401 aagcattctg ataatctagc ttatgataat gtggataatc taaaacctct atcttttgtg
     5461 caaaaaaagc aagtagttcc agttaaagca aaaaatatat ttcctattct cccttcattc
     5521 aaagctaatc acttcaaaca acaattaaat gaaattaatt atgaaagcat tgcagatact
     5581 gattctctaa caaaagtatt aataggatta ttgattttag ctctcatatt gatagtgctt
     5641 gatttgattt taccaggaga tgtatttgct attatatcta ctataatttt agttttaatt
     5701 ttagtctacg ccatatattt tctatttaaa ttattataat taaattggct tattagcgtt
     5761 aattataaaa atgatattat gagccaagtt aaaatattat gggtagatga tgagatagat
     5821 tatctaaagc cacacatcaa gtttttagaa gatcgagggt atgttgtata tacagctact
     5881 aatagttcag atgcattgga gatgctacaa aaagaaaatt ttgacattct ttttttagat
     5941 gaaaatatgc caggtatgcg aggtatgact atgctagaac atgctcgtag caaaaatatt
     6001 actattcctg tggtgatgat cactaaacat gaagaagaac atctaatgga ggaagcttta
     6061 gcacatagaa taacagattt tttaataaaa cctgtaaatc cttctcaaat tttattaaca
     6121 cttaaaaaag ttttagataa aaaagatatt caaaaaaaga aaacaataga tgattttcaa
     6181 caagattatc gcgaattaat tatgaatcta tctccatcta tgacgataga agaatggaaa
     6241 gaagtatata aaaagattgt ggactacgag atacgttttc agtcattaaa cgaaactatt
     6301 tttaataata ctatcttgtc gttaaaagaa gaggctaatg aggtctttgc taaatatttt
     6361 actcattatt atcctgattg gctcagtgaa gcggagacca ctgctataac gttttctcat
     6421 acattgctaa aaaaacattt attccctcat ttagataata actcacgagt attttttatt
     6481 ttgattgata atttacgtta tgatcaatgg gtaatgttta aacctatttt tgaagaatat
     6541 tttcaaataa tcaatgaaga tctatattgt agtattttgc ctactactac tgagtatgct
     6601 agaaatagtc tgtttagtgg tttattaccc aatggtatag ctcagctata tcctgatttt
     6661 ttagaaggtg atgaagatat aagttcaaaa aatcaatttg aagatgaact attcatgaat
     6721 tatttaaaaa gatatggaaa aaacctaaag gtagattttt ttaagatctt acatccacct
     6781 atggctcaga gatatataaa gtcgataaat gagcttaaag acaataacgt aaatgtatta
     6841 gtttataatt ttattgacac ctttgcacat gctaaaactg aagtagaatt agtaaaagag
     6901 ctagccaaag atgaggtttc atatagagct gttacatata attggtttat tcatagtata
     6961 ttgatagatg tcattaaatg ggcagccgaa aatcattttt caatatttct aactacagat
     7021 catggttctg tacaaataaa aaatcctata aaaataattg gtgatagaga aacaaataca
     7081 aatttaagat ttaaaactgg taaaaatctt tctactgatg aaaaaaaagt ttattgggtg
     7141 cgtaaaccaa atcaaatagg actaccgata caaaatgtga gtgatactta tgtctttgct
     7201 ctgaaatacg atttttttgt ttatcctaat aattatcaat attattcaag atattttgct
     7261 gatagctttc agcatggagg aatatcatta gaggagattt tggtgcctta tgtatttatg
     7321 acacctaaat gatgtttttt tttaatatct ttttttaata tataaggtgt ccaattgaaa
     7381 agctattttt tatttcgaat ttcatatact ttttgtaatg gacttacaaa ttttattttt
     7441 taccatctaa aagtcaatcc accgattaca ttaaaagtag cttgagggaa ataaccaaat
     7501 tctttatatt ctacacctcc atcatagtat ctatataccc aagcatttgt ttcataaaat
     7561 tcattaaaaa tattattaaa agttacaaat agattgattt tatcaaattt tttgcctttt
     7621 atagtataat caattctgat attgtgcacc ataaatggat ctatcgtaca agactcatct
     7681 cctgtattgt ctatatattg cttagagaca tatttagaaa gtagggatat agataatgcg
     7741 ggcaaaggtt tgaatgttaa tatgctagaa gcaactatag atggagaaaa gctaataggt
     7801 gtatttttta atactaaact tctctgagat ggccatagct cataatcatc tatatacata
     7861 gttatctttt tgattttatt ttcactaaaa gtgaaattag catcccagct cagccacttt
     7921 gtaaaatcaa aattccatat taattccacg cctgttctat aactttcagg tacattttgc
     7981 ataataggat cgcctacatc attaatttta cctgttagca ccaattgatt atcataatac
     8041 atataaaaag cattgatatt aattaaccca tttttaaatt tacgtgtata acctagttcg
     8101 agatcataga gagtttcatg tacaggtctt agactatcta catctgttag cattagatta
     8161 tccctattag gctctcgatg gctgatgcct gttaaaagat ataattcgtc attttgtgaa
     8221 atattataat tcaagcctag cttaggattc caaaaattat aaaattttcg taaagtatca
     8281 gaaataaaag catcgtcagt acctttgata gtgaaatcta catatcgata ttgaatatca
     8341 gcatatagag ttaaaaactc attaatatca taatcaaatt tagtgaaaat gctaaattct
     8401 ttttttaaac ttctatttct ataccattcg tgattgatag gtacattatg cgcatattgc
     8461 atccaaatga tacgtccata atgccatcca tcatattcat aataatttaa tcctgttctg
     8521 attcttagat ttttgttgtt attataaata tgactcatta tgattccata aaagtcattt
     8581 tctagaaatt ttctttgcac taaatccatt gatttgattg taatagtgtc atttctcgtt
     8641 gtatctatca agattactgg ttctagatta taatttgaga gttttttgtc ttttttgtat
     8701 tgttcataaa atccatatcc gttaatataa aagagagtag cattgaaagt atttttttca
     8761 tttaattttt gagttatatt caaatgatag tgatattgcc aataattgtc agtttcattt
     8821 tcgtatgtgt aaggattgta acgacgcaat ttagcatcat tgagatattc tttaggtacg
     8881 ccaccccaag cttggtaagt tttttcatat ccagccataa acaatccttt aataatagtg
     8941 tttttaccgt agtatccacc agataaataa gctgaattaa gctctgaaaa tgctctatcg
     9001 atgtatccat ctgaatattg atgagacaat ctgccctcta gataccaatt atctttaatc
     9061 aaacctgtgc caaattttgc tgaagcaccg aaagtattaa atgatcctcc taatgcttgt
     9121 agttcgacaa aaggatctgc tttcaaaaca ccagtaagta tatttatgct agcgccgaaa
     9181 gatgcagcac cgaaactaga tgttcctact ccacgttgta tttgaatatt atcagaagat
     9241 gatgctatat cgggaatatc tacccaaaat acgccttgag attcaggatc attatatggc
     9301 acgccattaa tagttacgtt gatacgagta atatcagtgc cacgaatacg tatatctgta
     9361 tatcctatac catttcctgc atcactagta gctactacag aaggtgtaaa atttaaaatt
     9421 aatggcaaat cttgaccagc ttttaaagat tgtatttttt ctattgtaac gtttgtataa
     9481 gttttaggtg atttgtcctc ttgtctgata gcacttacaa cgatctcatc agtcaaaata
     9541 gattttcgtt tgagagcgat taaattcttt tgctgttctt ttactaccac agtatcagtt
     9601 tcatagccca tgtatgaaat aattagcgtt actggtaatg attttacatt taatgtgaaa
     9661 ttaccatcat tacctgatac cgtacccaag taagtaccat caattttaat atttgcccat
     9721 ggcaatggtg attgtgtttc ttggtcaatt acttttcccg ttataataat ttgactattt
     9781 aagctgaagc ttaacatcag catgatgaca aataaaatgt tttgtttttt cataattccc
     9841 caatgttttt tgttttgcat tgggagtgtc agagacgata ccacattttt aggacccctc
     9901 cctacgccgg cattatccgg atcaggttct aagggtatga tctcagcctt ccaactgtat
     9961 ggaatagcac ccccgttttt ttctattgca aatatagtaa ttttttctaa tttgaataaa
    10021 aaattaatat cataattttg aaaaataaaa ttgtaattat aattattttt ttatttatat
    10081 ttgtatgtta aaaatttgag atagtgtcaa aaaaatcata ttttatattg attattagct
    10141 atttagtcat tataatttta ctaattttct tttggaatag ccaaaagaaa aaaattaaaa
    10201 agtgaactat tggattctat aaattttgtt tataatattt tgcctgaagc tttgattgat
    10261 agtgcttata agcaatctta ttaccctgta atatcttatg atagtttgcg aaatatttta
    10321 aataaaactt gtaatgacag agatttaaaa tatttatatt caataatacc ttatcgcgaa
    10381 gatacttttt tatttattat ctcttcatat acacacgaag atattttgaa tgatatgata
    10441 tctaaatatt tgatgccata ttatcactat cctgatgtat taaatacact ttcggctgat
    10501 aatcctatta attatacgag ttatagtgat gaatatggaa gtttttattc ggcttttcac
    10561 tattttcaga cttctaaagg caatccttat ataataggtg cagattattc attagacaaa
    10621 attatttcac tgaattggaa atttattttt ataattttgg gtgttacaat gatttttatt
    10681 tttatttata tttatcataa aaatttgaaa tataacaatg ggttgtagat ttttttgtaa
    10741 taaacattaa acaacaatcc aaaggcaaac acctaatttc ttattttaac aaatttcact
    10801 tttagtacat agacctttca ttagattact acataattgt atattataaa taaaatattt
    10861 taaaatattt tatttataaa aaaatcttta tacttttgca ataataattt tattttatga
    10921 aaaggtttat agtatatctt tttttattta atgttattag ctttgcattt gcccaaggca
    10981 atatttcagt taaaatcgaa gtaaatgatc tcaaagaaca taatgcaata gctgatgcac
    11041 aggttagctt aggtgaattt ataaagcaaa ctgatgataa aggaatagct acttttagtt
    11101 tattacccgg aatatatggt atcacagtga atgctgctgg ttatcaatcc tatactgata
    11161 tcatagactt attgagtgct aaggatacta ctattatagt acaattggca agaggcttgt
    11221 ctctatctat acaacaagag ctagcacttg agcaagaatt agaaataata aatgatgatt
    11281 ttgaccgcgg agcatctata aatccgcttt tgctaagctc gagagatcct tttttaaatt
    11341 tagcatcata taacttgagt tctggttcgt tttctccccg aggcattgaa cgtggtggtt
    11401 atgaggcata cattaatgaa gttttacttt acaatcccga actcgacaaa ataaattata
    11461 cagattttag tggactgaac gatgcagtca gatattctga tgattcttat ggattagagc
    11521 cattatattt gagttttgga gatattatgg gagtttctaa taaattggct actccatcac
    11581 gtatgagact cggtcatcag cttagttatg gctttagtaa tcaatcatgg caacatcgac
    11641 tgatgtatac atataatagt ggtataatga agaatggatg gggctttgct gtatcctact
    11701 cacatagata tgcttacgag gcgtacattc ctggtacttg gttgcaagct gatgcatttt
    11761 tcttttctgc tgaacgcaaa ttttctgatc gacacagttt atcagctaca atatttgctt
    11821 cacctcaaaa aagagctatg gctaccccta ctgttaaaga agtgcaagaa ctagtagata
    11881 atccatatta caatcccaac tggggcgtcc aaaatggtga aaaacgtaat gctagggtta
    11941 gatctatttt taaaccatat ctaatcataa aagatgatta taaatttaat gataatttta
    12001 cctggtctaa tgcttttggt tttacttatg gtatttatgg tactactaga ctcaattggt
    12061 ataatgcacc tgaccctaga cctgattatt atagatacct ccctagttat caaactgatc
    12121 cttacatagc tgaaatagtc acacaacagt ggaaaaatga tgtttcagtt agtcaaatta
    12181 attggaataa actatatcaa ataaactatt tagcaaattt agaaaataaa tctgctcgat
    12241 atatagtaga ggatatacac aacgacccac tttattttta ttatggtagt aatattaaat
    12301 atacttttaa tccatctagc attatttatg gaggcttaca ggcatatcat tatgaaactc
    12361 ataatttcaa aaaaattgat gatctgttag gcgctaatta ctatttagat attgataaat
    12421 atgctgagag agattttcct gataatcctt atatgcttta caataatttg gatgaaccag
    12481 ataaaccctt gtatgaaggc gatgtttttg gttataacta taggttaaat acaaacgaaa
    12541 taagcttgtg gacacaatat aagcaaactc tacctcgaat agattattat gtggctggta
    12601 aaataggttt tagcaataaa tatcgaaatg gtttatacag aagtggtagg ttccccgata
    12661 atagtaaagg taaatctgat caagtaaatg attttattgg tggattaaaa actggagcta
    12721 tttttaaaat ttctggcaaa cattatataa aagctaatat tgctggattt acaaaaccac
    12781 aatctataat agatattttt ctttctccct ataatagtaa taggcttaat aatgacatta
    12841 caacgcaaaa agttttatct ggtgatatag gttatcattt taattttaaa tttttacgtg
    12901 gtcgtataac tgcttatcaa acatatattc tggatcatat ggatatgcgt ggtttttatc
    12961 atgatgaata tcaaacttat gtatacatgt ctttatctaa tatggacgaa ttgtatcaag
    13021 gcatagaact tggattagaa gctaatataa tgccagattt atatatttct gctgctggta
    13081 acttaggtaa ttatagatat atcagtagat ttaaagcaaa cgttagcttc gataatctct
    13141 cgcgtccaga tactagttat actgtatata gcaaaaattt ttatattcca aacgtacctc
    13201 agagagttgg tagtatagct attaattata aaccaaacta ttggtggttt acacttacat
    13261 ttaattactt cgatgagatg tatatggact ttaatcctga gagacgtaca caatttgctc
    13321 ttaataattt aggcccagaa gatccattaa taaaggatat tattaatcaa aaagaattat
    13381 atagcggatt tactcttgat ttatctatta gtaaaacttg gatgataaaa agccaatatc
    13441 gtttgggtgt taatttattt ataaacaata ttctagataa tcaaaaatta gcactatttg
    13501 cttttgaaca gtctagattc gattttgata ctaaaaatat agataaattt ccccctaaat
    13561 atatgtatgc ttatggacgt acatatatgt tattaataaa acttagtttt taatcaataa
    13621 aaaataaaaa tatatgaaat ttataaaaaa ttttattgta attgcgagta tgatattatt
    13681 attcgcagga tgcgtggacg atgagtttga tgttccacca atagatattc cacatgtaga
    13741 tttcgaagct aatactacaa tagctgagct aaaagctatg tatagtggag gcttagatag
    13801 tatagatact aatattataa ttactggtgt agtagtagca aatgacgagt ctggtaattt
    13861 ttataaacaa ttagagatac aagacaatac aggtggcata gaagtgagat tagatagata
    13921 tgatttatac actcaattta aggttggaca gagagtatac attaaatgtc aaggtttgtt
    13981 tttaggcaat tatggaggct tgattcaact gggttataaa taccagggag gtataggtcg
    14041 tatacctgat gctatcatag atcaacatgt tttcagagac agccttcctg gcactccgcc
    14101 acctcctatt aaaacaacta tacctggatt aaacactaat tatcttagta tgctaataca
    14161 aatagactca gtgcatttcg gtgaagtagg agctaaattt gctgaatccg atgctatcac
    14221 caatagaact atattagata gagaaggaca tactttgatt ttgcgtactt ctaattatgc
    14281 taatttcgcc tcagaattat taccacccgg tgaaggcagc atcagaggta ttttatctat
    14341 ttttaatggt acttatcaat tatacattag agatttgaat gatttagtaa attggaatga
    14401 agatgcaact ttgccatttt tagaaacatt ctcttctaat ccattagcta atggtacatg
    14461 gctaacttat agtgctgcca gcaacaaaaa ttggacttat aatagctcaa atggaaatcc
    14521 aggaggatgc atggaaatga atggctacgg tgccgatgta gcttcagatg attggcttgt
    14581 ttctcctgaa tttaatctaa caagtgctac tgcagcttca ataaaattcg aatcttggac
    14641 acaatatagt gataatggca tcacacaacc tttgcaagta tatatattag aaaactatgc
    14701 tagtggagat ccttcacaag caacagctac aaacatttca gataggtgta ttatgccaga
    14761 agctaatagc aaagtatgga tttctagtgg tacagtgaat atttttgatt ttattggtaa
    14821 aaaaattaga gtagcttttc attacaaatc tagtggcact ggctcgtcta catctacctt
    14881 atggagaata gataatgtag ctttaaatta ttgaaaataa ttaatatcta caataaaaca
    14941 aaaactctat atgagtgata gactagataa aaatgataag atgtaaaaag tatgaatttt
    15001 tttagtaaaa aactttgtaa cttgtatcaa gtgctaaaat tgatatatat atagtttcta
    15061 taacgtttat tgtgggtaaa aacattttat attgttataa aaaaataact attttttaat
    15121 tatttaggtt aattttttta ccacaaaggg ctatttgtag cctttttaat tccatccttc
    15181 tctgcgttct ttgtgcattc tttgagaact ttgtggttaa tatttaacta cactataatt
    15241 aataaactaa tattaacaat ttttttataa aactactctt ttttttaatc tttacccata
    15301 caaaacgtta tagaacctaa atatttttta aattagtatg tattattcaa atataagtca
    15361 gtgtggttat aaattaatat gtattaatcc attaaaaaaa attatagtaa attcgtagtg
    15421 aaattttgat aaggtgtata gattaaaaac taattttttt gtaattttat taattatata
    15481 ctctacaata gcaaatgctc agttttatag tgggagtcaa ttaaattttg gtaaaaatcg
    15541 agtacaatac cgctatgaaa ggatctggag tgtgatgaag tttgattatt atgatatata
    15601 tttttatcaa aaaggttacc ctatagcagt aaatacagct agatatgctg attcaataat
    15661 tcataattat gagaataagc tcaattatca tttaaaatct aaacttcagt ttattgtttt
    15721 taattctttg tctgatctga aaagcagtaa cataggcata gatgatgagc tactatataa
    15781 ctcggctgga caaactataa tagagcgaaa caaaattttc ttgtattaca atgggagcta
    15841 tgtagatcta tttaaacaga taagagaagg cgtagctaga gtaatggttg aacggttttt
    15901 gtatggtgaa accttcggtg ctaatttcaa aaatcagact ttggatgcta taccttcgtg
    15961 gtttattgat ggtttggtat cttatttagc tgagccttgg agcactgaaa tggataattt
    16021 attaagagtg aatattctag ataaaaaatt taaaagattt aatcagttag aaggtgagca
    16081 agctcgagta gctggtcata gcatctggcg atatattgta gatatatatg gtgaaaatgt
    16141 catttctaat gtattatata tgacccgtct aagcagaagt gtgagtgatg gtatcattta
    16201 tagcttagga atcacataca aaaacttcat aaatgcttgg tataattatt atgaaaaatt
    16261 atataaaaac gaaatagagc aatgggaagc atgggaaaac atgtatacat tacctatttc
    16321 taaaaaagat aatccatata aagtagctct aagtagcgat ggtcaatatg cagctatagt
    16381 agcagataga atgaatcaaa aacgagtata tttggtttct atacaaaatc caaaagaaaa
    16441 aaaattaatt ctaaaacttg gacctaaagt agaggaagcc tttgatgact cttatccttt
    16501 gatagctttt catccgtatg cacctctact ctcctgctat tatgaaaata aaggacaacg
    16561 aatgctcatg ctatacaatg tagatgaaga tactaaggaa tatattaaaa tagaaagttt
    16621 agacaaaata acttcaatat catattcgcc ggacggaatg aatttagccg tctcagctat
    16681 caatgagggg caaagtgata tttatttatt ttcttttgct ggcaaaaact ttactcccat
    16741 tactaaagat cattatgatg actatggacc tgtttttatc aatagaaatc aaatagcttt
    16801 tttatctaat agaactaaag atacattaga atatgttaga aaaacatata aatatgatta
    16861 tttttcatgg cgaaatacac caggtaacta tgatgttttt atttatgata atcgtaagcc
    16921 agatatacta atccagggga ccaaaacaag tatggtaaat gaaaatgaaa tctttgctca
    16981 taccaaagat gattttttat ttttaagtga tagtaatgga gtaaataatc ttttcatagc
    17041 taaaatagat agctcattac tcatagtaga tacagtgatg caatacaagt attttgcaga
    17101 ttataaacca attacaaatg taaataattc gatattaact gctagtattc atgataataa
    17161 agcattgata ataacaaaag ataaaaataa caaaaataaa atttcccaag taagtttact
    17221 ttttactcct aaagatagtt taccacttag ctactataaa aaacaacaat tgaaaaaatt
    17281 gtttgcaaaa agtaatatta ataaaaaaca aattgaaacc aaaacaatag atgctcataa
    17341 gcaaaattat caaattattg gcatagatga taaaaatgat acgttgatag atatttacaa
    17401 ttataaatta gaacaagata ccataacaat agataaaata gaaaaaaaag aatccaaaaa
    17461 gcataaaact aaattattaa ctactatacc acacacctta gatgattttg cactacctaa
    17521 ggtagaaaca tacgaagtag cttattacat tgatgaggta actacacaat ttgatttttc
    17581 gtatctaagc tccacatatc agccatatac tggttatggg cccatattta ttcaacctgg
    17641 tatgaatgca ttgtttttat ttaatttgac tgatttgctc gaggatcatc gtataatttt
    17701 aggcacacat ttatcaggtt ccttggacga aaacgagtat atagttagct atgaagattt
    17761 atctcgtaga gctgataaac aaatagtttt tcatagaatg agttattact caaatgaaga
    17821 ggaatttttg aaaaaaaata ctattcacga tatacacttt agatggaaat atccttttaa
    17881 tcaagtatct gctttgagaa tagatatttt cgctaaatat gataaaatag catataaatc
    17941 tatagataat gtaagtctca tgcaacccga tgaacatgaa ttatgggccg gaggtgtcat
    18001 agattttaca tacgacaata ctagaaatcc attaataaat atatattatg gtacgagggg
    18061 aaaagtattt tttgaatatt atcaaggcgt attaaatcat aaattaaatt tatttaatgt
    18121 aggttttgat tttagacatt atactaaaat atatcgtagt ctgatatggg caaatcgaat
    18181 agctggcggt acatcttttg gttccacacg tttaatttac atattagggg gagtagataa
    18241 ttggttattt cccagataca tgtatcaacc agttttggac ccatcaatga atttttcttt
    18301 tcagacctta gctactaatc tcagaggtat gccgcagaat attagaaatg gcaatacatt
    18361 ttttgttatt aactctgaaa tacgttttcc aatatttaga tttttattta ataggccatt
    18421 gaaatctaaa tttcttaaca cgtttcaatt aaactttttc ggtgacatag gttcggcatg
    18481 gttgggttta aatccttatg cagacatcaa tatatacgtg cctgaatatt attataaaaa
    18541 accaatcaca gtggttatca ataacgctcg aaatccgctg gttgggggca taggtttcgg
    18601 ggcaagagca gatttgcttg gttactttat gagattagat tatgcatggg ggatagagga
    18661 gcgaggtatt ttatctccta gattgatttt ttctataact aaagattttt aataagtcat
    18721 aaaaattttt ataaattgga atttataaat gtatcgaaat taataattgt tatacttaag
    18781 gtagttctat ttacgaaagg cagattttat ctagtttgtc gtgtacttat gtgtgattac
    18841 aaaccacatc agatagctct ggaagtattt ggtagctact cctctgaatc actgtaacca
    18901 aatcttgaaa tcattaatat gctaattgac tacttttaca ctgtgtacac agtataaaat
    18961 acttgaagta taacagatga taatttcggg ccccacaaac tattttttta gaaactcaat
    19021 aaaagtttca aagtatagtg atattatttt atataaattt atgttaatta aacagacgtt
    19081 tatttaaata ttttttttaa attcgtaaaa aattaaaatg aaggctggta gattaggttt
    19141 cggagaaggt gttttagatg ctggtaaaaa atatgataat ttagtggttt taggtgcaga
    19201 tattactgca tcagtagggt taaatctatt taaggaacat tttccaagca gattttttag
    19261 catggggata gcagagcaga atatagctgc tagtgcagct ggcatggctt tgtctggatt
    19321 aataccagtt tttagtactt atggtgtatt tgctgctttt cgttgtgcag atcaaatacg
    19381 tgtatctatt tgttataata atgcaaaagt aatcataggt ggtgctcatg ctggtgtaag
    19441 tgtaggcccc gatggtgcta ctcatcaagc attagaagat atagctgtta tgcgtgcttt
    19501 accaaacatg acagtgttat ctccatgcga cgctaatcaa gtaaaaaatg ctgttttagc
    19561 tgctattgag caaatagatg gccccgttta cattcgtttt ggcagagagc ccttacctaa
    19621 tttttcacca gaaatagcac atctacaaat aggtaaagct gaaatactaa atgatggtac
    19681 agatattgca attattgcta caggatcgct tgtgtgggaa agcattgaag ctgctaaaat
    19741 tctttataat gaatataaga ttaaagcaat ggttattaat atacacacta ttaaaccaat
    19801 tgataaaaat acattaatag atgctgcaca aaaatgtaac attatgatag tagccgaaga
    19861 gcatcagaaa tttggtggtc ttttttctgc agtctctgag gtgtgttcag aatattaccc
    19921 tgtccatatc aaatctattg ctatgaatga tagatttggt gaaagtggga aagctgatga
    19981 actaatgaag tattatggat ttactgctac taatattgtt aataatgcta tcgaaatgct
    20041 gaataaatat aaaaaatgaa acgatttcta attatttatt ttattttttt tgtaattaca
    20101 aatttaaaag ggcaaatgga tgagaaatat ttattaggca aatttgatcc tgctgatgat
    20161 agtaattttg ttttagtaga caaaaaatac acttctgatg ctagagacat ctacctaaga
    20221 aaagaagttt atgaagcatt tatacaaatg cacgaagatg ccaaaaagga cagtataatt
    20281 ctattaattg ttagtggtac gcgaacttat gaatatcaaa aaaaattgtg ggaagataaa
    20341 tggtttggtt atacatttgt aaataataaa gatttatacc tagccgtacc tgatgattta
    20401 gaacgtacaa aagaaatact aaaatataca gctatgcccg ggacttctcg tcatcattgg
    20461 gggactgata ttgatattaa tagtgtagag attgaatact tccaaactga ttacggaaaa
    20521 cttgtatata attggttgca aaataatgct gaaaaatatg gattttgtga gccttataca
    20581 gattttgata gcatgagacc atacggatat gagcctgaac catggcattg gagctattac
    20641 ccattagcta agctgatctt agaagattac actaaaacta ttaaatttta tatgatgatt
    20701 gatttcgctg gatttgaatt ttttaataaa ttagaaataa tagacaaata cgttttaggc
    20761 atcaatccta aatgtaaata aaaattttat ttataacagt aatatttttt taattatttt
    20821 ataaatttaa tatgtgtacg atacatccta aatgcggggc atgcttgtat tctactgcga
    20881 catataataa tatatggaaa acaaaaatta atactgctat taaaaactta aaaccatttg
    20941 taagtaataa tgttcctata attatgcacc caaaagatgt attacactat agaaaaaaaa
    21001 ttcttttaca cgttaagtct gcaattattc cccctaaaat tggattaatt caaaaatctg
    21061 atattgtaaa tatagatcta tgcccgctac aaccagaagc aatcaataaa aaaattgata
    21121 ttattaaata ttctttgcct caatatgata aatttccact acaatatatt tcttttaata
    21181 atgagactgt tattttagtt ttgaaggacc atcactctca tttagcaagg tatttagata
    21241 caactaaagc aattttttct aatgttgact ttccttattt atatcttcat ttgaatccct
    21301 cgacaggcaa taaaatattt ttaaaaagtt atatttatcc agtttatgga gatttatttt
    21361 taaaagatgc tgatggatat tggtatcatc cattagtatt tcagcaacaa atacattctt
    21421 tgcatataga aagtttgcaa atagctatag attttttaaa aggtatagac aattttttag
    21481 atttatattg tggtatcggc atagcatcat caatagcaat aaaatatttt aaaaatattt
    21541 atggaataga acatttagcg atatctattg aatgtgctaa aaaaaatgct aataaagcaa
    21601 ggttttttgt aggacaagta gacaacagaa tgaggactct cgttaaaaaa gagcctgatt
    21661 ttttcgaaaa ttatgtaatg tatactaatc ctcctagaag tggcatgggt aaacaatgta
    21721 taaaagaaat attaaaatca cctcctagca agatagctta tctatcgtgt aatactaaat
    21781 ctttaaaaaa tgatttgatg ttgtttaatg aaaattataa tatcaatgct attcattttt
    21841 ttgacttttt cccatatact aatcatatag aaaccttggt actgatgagt aaagagtgta
    21901 ggtaagacta agagaacatt ataaactgat actaataaaa atatagcgta atggttagta
    21961 aaaaatcacc tcatctattt taacatcatt atcatcatat ggtatgtaat cgagtatctg
    22021 ttcttcaaat gctataccta tagtataagc atttaatttt tttaaaaatc tatcataata
    22081 tcctttacct ctacctaatc tatgtccatt gtgatcataa gctaatgcgg gcactaaaac
    22141 taaatctatt ttatcataat cttgccatgg atcaccgact ggttctatga taccatattt
    22201 gttggcaata gacatgtatt ccatacccat aaatcttact ggcacaatat tgtctccatc
    22261 tatgcgtggt aataaaatta tcttttgaaa agaccaattt agcacaaaat catgtgaagg
    22321 caattcatcg ccaaaagacc aataagtcat tacgaccttg gcatttctaa atcttggatg
    22381 caatgagact ctatcccaca cttgcgatgc ttttttttct cttgagtctg gatttatatt
    22441 atttatcaat tgctttatat attttcttgc cgttttttta tcttttaatt cttttatttt
    22501 caaattatcc atagttatct aattaaattt ctagaactaa ttttttttag aatttggttt
    22561 tctaaatgtt ttaaaattgc aaattcttct agtaattttt cttgttcttc ttgattatta
    22621 gtttttttta ttttttcacg tgtttcattt tttagtttat ctagtattag agctttgtat
    22681 tccaagatag aacattccac ttcataataa taaagtaaat cattattttc aatggtattt
    22741 acctcaattt ttaatttttt ttcccaattt tcactaagtt taaaagtatc taaaagcaaa
    22801 tcgtacatta aatcttgctc ttctggattt aaatcactga ttatattatt tatattaaaa
    22861 ttattaaatg tttgatactg atctattatt agctcataaa gatctttata aaaagcaaac
    22921 tcaattttat cgagttttag ctcttgatta atatatttcc aagtaggtat atctatttga
    22981 gtatcaaagt taaattcatc tttttctata aaagttaata tcctattacc gaaatgtata
    23041 agtatgtata atattctttt ttcttgttgg ttaattttat tgtttataga tgataaattt
    23101 ttttgatttt ttaatttttt ctttggttta tcagttatgg gattgtaatt ataagtattt
    23161 tgtagttgtt tttttaaacg atctcttaat attctttgaa attcttctct aagagtaaat
    23221 tcatcaatat caaaattttt tgaaatataa actaagtatt ctgcttgtat taatggatcg
    23281 gaaatatgag agagagagaa taatatatta cgaatagcat cagctttttt gataggatca
    23341 tttgttttgt cacctattaa atggtgagat ttgaaattga taaaattttc tttattagtt
    23401 tttaaataat ctatgagttc agtagttgag actttatgag tgaaggaatc tggatcttca
    23461 ccttctggga atgagataag atatacattt aatccttcct ccaaaagcat atctatagct
    23521 ctgaaagaag cttttatgcc ggcactatca gcatcataca tcagacaaac attattagta
    23581 aatcgatgta tggtttttat ttgaacttgg gtcaaactag taccagcaga agctactaca
    23641 ttatctatgc cagcttgtac gagtgaaata acatccatgt agccctctgt tatgtaacat
    23701 aggtcttgtt gtgaaatatt ttttttagca ttattaagat tatatactat gttgcttttg
    23761 gtataaatga gcgtttcagg ggaatttaca tatttagcaa tattcttatt atttccgata
    23821 gctctaccac caaaagcgat aaccctacca ccaacattaa aaatcgggaa aatcaaccta
    23881 cccctaaatc tatctgctaa gctatctggt attattaatc ctgcatttat tagatcctca
    23941 tctgtaaaac cttttttcaa agcatgattt ctaaaagcat cgcgttggtt gagaccgtaa
    24001 ccaagattaa atttctgtat ggtatagtcc gaaataccac gatgatgaag ataagataag
    24061 gcaatatcta caccttcttc actactccat agttgagctt taaaccattc gagagctatt
    24121 tgatttaata taaagatttt ttctttttca tctttttctt catctaattc gctaccatct
    24181 tcatctacag gtatttgata ttttttagct atagtacgta tggcttctgg gtaagacagt
    24241 tgttctattt tcattaaaaa agttattaca ttgccactct caccacaacc gaagcattta
    24301 aaaatacctt tatctggagc cacactaaat gatggagttt tttcattatg aaaagggcac
    24361 aaaccaaata agttattacc ccgtttatgc aatgttacat attctttcac tacctcatct
    24421 ataggcacat tatcaagaat aaaatcaata gtacttcttt tgatcataat acacaaagat
    24481 aaaaaaaatt gttggaatta aaaaaaatat tatatcttta taaattaatt tttagtcaat
    24541 attaatctca agtttattaa ttatttctaa taatattgtc tgtaaactta ttttatggat
    24601 aagacaattt attgattaaa atttatatcc tttggtttta gtcctagttt tttaccatat
    24661 tctaacataa atttataagc agcatcatac tcgttaggga tctctccatc gataatagct
    24721 tctctgatag cagttttaat atcacctacg agtttagatg gcttcagatc gaatgtttgc
    24781 attattactt cgccactgat aggaggttga aaatttctaa gccgatcttt ttcttcaatc
    24841 tcatcaaatt tttttttcaa attttttaaa ttatctgtaa attttttaac tttccgaata
    24901 ttttttgatg taatgtcaca ttcacataat atcatcagat catccaaatc ctcaccagca
    24961 tctaccatta atcgtctaat ggcactatct gttacattgt catctactaa tgctataggt
    25021 ctcagatgta gtagtatcaa cttgactaca taatccattt tttcatttaa tggcaaatgt
    25081 aagcgtttaa aaataatagg aaccatttta gcacctacga tctcatgatt atgaaagcta
    25141 aagccagttt tctcgtcgta tcgtttggtt tttgctttac ctatatcatg caaaagtgct
    25201 gcccaacgta gccaaagatt gttaggatga tcaggtatat tttctaattg ttggcacaca
    25261 ttatccaata cttgtagtgt atgcttgaaa ttatctttat gagagaaatc tagttctgta
    25321 tctattgttt tcagagctgt taattctggt aatatataat ctaaaagtcc cgaatcattt
    25381 aataaattaa aagcaataga aggtataggc aataataaca ttttgtttaa ttcctctgat
    25441 atgcgttcag gtgctacgat agagatgcgt tctttattgg cttttatacc ttcccaagta
    25501 ctagcttcaa tatcaaaatt taatttacta gcaaatctga tagctctaag catacgaagt
    25561 ggatcatcag aaaaagtaat attagcgtca ttaggagtac gaatgatacg attgtgtaaa
    25621 tcatcaaatc cattaaaagg atcccagact ttaccaaact gaggccatag ctctattcct
    25681 atagcattga tagtaaagtc cctacgaaga aaatcgtctt cgagatcagc aggttctaca
    25741 tcagggttgc gactatcttg ccgataactt tcctttctag cggaggttat ctcccattct
    25801 attcctttat aaacaaaatg agctgctaaa aaacgcttaa aaaatactaa attatttatc
    25861 tgcaattttt cacagatgaa ttctgctaat ttttgccaat caccttgtat gactatgtct
    25921 atatcctcta tttctaatcc taataacgaa tctctaatag cacctcctac tacgagtgct
    25981 atattattgt tttctttaca aaattgttga actaatttaa tcacctcgta gttttctaat
    26041 tttgatgaga tagtctcaaa aataatcata ttcaattttt tttttaaaaa tgatatttat
    26101 attgaaattt tattgtgtgt actaatttct ttttcacatc tccacttatt tttctatttg
    26161 ctcgctcacc ttgattgcta gtctctttat tcatctaaat ccttttgcca tctgataatt
    26221 ctaccatttt tacaaaatgc ataacaaggc aaattatctg gtatatcgca ttctatcaaa
    26281 tttttttgta tcaataaaat ctcattacta tccaaagata catacttgac accagaacga
    26341 gcagtccata ttttaaaatc ttttatataa atatagcctg tataataacg aggtataaat
    26401 ttccccattt tataatgaac atcaaatttt agctcatcta taaaatcagc cggtcgagct
    26461 gggcgacgta aaagatttac attagattta gctcgagtaa tagcagtata tagccatcta
    26521 agaacaaaat ctgatggttg tttcgaatga taaagcatag actctgttac aatagaaacc
    26581 tcttcccatt cgcctccttg agccttgtgg caagtaatag cataagcata tttaagtctc
    26641 agtgcattta gatatggatc attttgcaat ctaaacatta atgaacgttt attgtccaat
    26701 aaatcgggat tttgtttttg aattttttca aaaatgtagc ttaaaattaa tagctcatcc
    26761 tgaaaagtat aattaaaatt tggttctaat gtattttcta gtaccataca cttcattaca
    26821 tcattatcat cggacccata ttttaattta acttttctaa aacgtagatg ttctatacgt
    26881 ttgcattcac tttccatatt tattatttgt actatctcac cattcataag atcatacaaa
    26941 taattatttt ttatcaccat tacatattca ttattattta ataatttttt agtatttttt
    27001 aaaagtttag aacgaatttt tttattaaga gtcgatacca cattattagt ataagctatc
    27061 atagttgttg gcaaagtggg cttatttttg agaatatcaa catgatgatt tatgatttca
    27121 tctacactat catagagcgt tacatttcca ttctcttcta tatttatatt agattgatta
    27181 ccagatgtca aagtatcacg gatttggtat gctatttgat agatacctga atatttagaa
    27241 aaacgtactg gttcacgtaa ttcaaaatac tggacaggaa agaaatattt catttcgaaa
    27301 agttcggggt ctaatgcaag agttttatgt tcatttacag gaggtagttg atatggatct
    27361 cctacaaaaa ctattttagc attagtgcca aaagtcaata tatcatctat caaatttcca
    27421 ctaccaaaga ttacattatc atcttcttga tttaatgaac tgatcataga actctcatca
    27481 atgaagagaa cagaataagg atctgtcagt ggatcagcta ttttgtaata ccaacgtaat
    27541 ctactacgcg atacttctga tttattatca tctagagtat aaatcagttt atgaatagta
    27601 caggcagaat attgacaacg attttctaac attctagcag ctttaccagt agtagcagtt
    27661 aagtatacac taattggctg cttcggtaat tttattggca ttaatatatt attttctata
    27721 ctttcattga tgatgggtat tgtttttatg agtgcttcta ctactgtagt tttacctgta
    27781 ccagccgaac ctgtaagcat aaaaatgtga ttatcctttc tagttggttt tagaaaggca
    27841 gacaatagat ttataaaagt tacttgttga tctttagtag gtgtttttat tattttacta
    27901 agtaaattat ttatatacaa agtaatattg ctcacagtga tatagtttgt ttttttttaa
    27961 aagatcaaat atacatcttt ttttgttaat aaaaaataaa taaaatgatt tattaatagc
    28021 tatcataaat aaattaaaat agtatgttat tggaaaacaa tgagttaatt tacaaaattc
    28081 taaataatta cattattaat ataaagggaa atggaaacat gacgtaagtc tattgctttt
    28141 tattattttt aaacggataa tctatattat aatgaagtcc tacactctct ttacgggcta
    28201 gcgccatagt agtaatcaag tatcccacta aaatcatatt tcttaattca caaatttgtc
    28261 gatttataat agattttttg tatagttctt cagtttcttc atgaagcatt tgtaatcttt
    28321 ttattgctct atttaatctc aaatttgatc ttacaatccc tacataggct gtcatggttt
    28381 gattgagttc ttttacctct tgagttatca atactaattc ttcatttaat tttactcctt
    28441 catcgttcca ctctggaata ttagtattta cagaataatt ttttaaattt ttttctacat
    28501 ctaaagcggc tctatgacta aaaacgaggg cttcgagtag tgagttagaa cctaatctat
    28561 tagcaccatg aagtccagta cgagaaactt caccacaagc atacaaccta ttaattctag
    28621 tccttgcata gtgatcaact acaacaccac ctacttgata atgagctgct ggtactacag
    28681 gtatcatttc ttttgtcata tcgatcccaa tgcttaagca ttttgcataa atagtgggga
    28741 aatgttctaa tagttcatct tttttaatat gcctacaatc gagataaaca tgttcagatc
    28801 cactaatttt catttcgttg tctatagctc tagctactat gtcccttgga gctagagatt
    28861 tacgtgaatc atatttatgc ataaattctt tgcctttaat atcttttaat atggctccat
    28921 aacctcttaa agcttctgta atcagaaagg ctggtctttc ttttggatag tataaggcgg
    28981 taggatgaaa ttgaaaaaat tccatatcca ttatttgagc ttttgctcga taagccatag
    29041 ctacaccatc acctgttgca acagttggat ttgttgttgt tagatataca ttaccactgc
    29101 ctcctgtagc taaaatagta aatttggaaa ggaatcgttc cactttgcct tctggcgtga
    29161 gaacataagc accatagcat tctatgtctg tcattgcctc atacactttg attcccatat
    29221 gatgttgagt aatcaattcg actgcaaaat aatgttcgaa tacatctata tttggatttt
    29281 ctaaaacttt atttgataag actctttcaa tttcatatcc cgttttgtct ttaaaatgaa
    29341 aaatgcgtgg ctcactatgg ccaccttctt tagctagatc atattctccc gttacttttt
    29401 tatcaaacct tactcccaat tctatgagtt cttttattct ttctggactt tcttttgatg
    29461 ttatttctac tacttctcta tcattcagat atgcccctgc ctctaaagta tcctgtatat
    29521 gtttatcgaa attatcaggc ggatacatta ccccaactat accaccttga gcatgatagg
    29581 tagaggtgtt gtgtagttct tcttttgtta ttatgcctac actacctatt tttgatgctt
    29641 ttaatgcaaa atttaagcct gctgcaccac ttcctattac taaaaaatca aatatatgca
    29701 tttctaaaaa tttttataag ataattacaa atttacttat gttttttgaa tgtattacaa
    29761 atattaaaag tttatatgca aaatcataat taagcaatgt ttacatccat tataaataaa
    29821 tatttatgtt tttagtttga aatatttgct ttatttaaaa aatatattaa ttttgtacaa
    29881 atttatttat ttataaagat ataaaacatg aaaaaattaa ttttactttt cattttgatg
    29941 tggattagct ttaattccat ctctcaagtt tatttaataa ataaaaatta ttgcatagtt
    30001 acttctaatg cttatttgat agtaaatggg catttaa
//
LOCUS       DAOPRN010000021        29924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1218_length_29924_cov_110.269309, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000021 DAOPRN010000000
VERSION     DAOPRN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29924
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1218_length_29924_cov_110.269309"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1407
                     /locus_tag="PKI65_03850"
     CDS             142..1407
                     /locus_tag="PKI65_03850"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOB77609.1"
                     /translation="MVKINTKKFFDQISPKSYYDIAIILPLLMLFIYPSRPAIGVAGF
                     ILFTIFGIIKKDLTFQWNANILWVIFFLLYVIFFFKTTNKQAGLFELEKKLSLLVFGS
                     LFLFKYNKPISIPFWIISFIILIFIRGIINFIEAFVMYRDIHLFQAFLTTRFSQHIHP
                     TYFSIYIIVSLFGLIYLQHKRFIKLPKWLIICYIIFSILLIIFLSSTAAILFFLFSIV
                     FFLIFYFYKKLKPLIATIFIITILLISFITIKYIPFKTINLKETIENTILFLNNPKEC
                     TQSKEFIESSNEKRLILWKLSWEKFKENPLGYGVGSAGDVYRDVYQKYELDSFIEPLL
                     DSHNQYLRIALEMGIIGLLAYLSILITFIVKAIKQKKYLLLFVLISLIYFGLFESVLQ
                     RQSGVIFFVYIITFLYLYEPNLLDYNKET"
     gene            1412..2467
                     /locus_tag="PKI65_03855"
     CDS             1412..2467
                     /locus_tag="PKI65_03855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB77610.1"
                     /translation="MKKILVVSSNSIHLFNFYNLINSYFDHIDIVTDSINEKYNYRNI
                     VLYKYNFSIKKPFQFIISYFKLKKLIKTNKYSIIHIQQITTAALLAILASKKSKIPIV
                     ATAWGSDILLISHRGYFYKKLVKYVLKNVDIVTADSEFVIQEMNKLTKQNIDAHLVIL
                     TNLHSTPKYFPKKSKIIYSNRLLTPLYRIDLIINAFEKFIKDKQYEEWKLIIAGQGNE
                     RHKLEKIVREKKLQNQIEFVGWQSTDKNNEYYQKALIYISIPISDGTPTSLLEAMSYG
                     CIPIVSDLPVYYEWIENNVNGLIVNDLNSNFIEKALKLNPIKVWEINRNIIEKKATID
                     VNKSKLEQIYNKLLQTK"
     gene            complement(3098..4618)
                     /locus_tag="PKI65_03860"
     CDS             complement(3098..4618)
                     /locus_tag="PKI65_03860"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB77611.1"
                     /translation="MRKILVFGLLFLGILVNIAAQDKIAAPTLVSPADSSNIDVEVIL
                     DWSSVSGAVSYKLEIDTSNNFSSPLKQTIETTYSAHTVKDLLYGTTYYWRVSAKNVNN
                     LYSSPSEVWNFTTKASVTLTIPADNSKKLPVKFTFRWNAIKGSKYLFQLDTTSDFNSL
                     LKKEYSELNIDTIDLFQLNYGQNYYWRVKAYHDYDSSEWSDVRFFTTKGDVIAKYPTD
                     NFEGFIPGDSIIFHTIFGSQYGELIISEDSSFDVQFYYIFDSTKIARIKVSNNPPKYD
                     TLTKVATGDLKFNQDCYWKARLFHAKDTSDWSDVYHFKTIDTLGLLLPSNGAQDINPS
                     SDSLSWSSIYAADEYIIYYDTLSNLSTSKTVSINSQDTLYNRKDKFVYYLRNLNLKYN
                     KTYYWAIKAKHVRDELMSDTWSFETIDNTSINNYQSSNFTILPNPASDYVKVNFNSEQ
                     TGAIVIKNILGQEVYREKINKAVYTTIPISSLDNGIYIIEFNGIDVYFAKKLVVKH"
     gene            complement(4631..7309)
                     /locus_tag="PKI65_03865"
     CDS             complement(4631..7309)
                     /locus_tag="PKI65_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB77612.1"
                     /translation="MRRLKFLLSAMLVIALFISGNAQQNNVKNNQKNTPLKSQGLQWV
                     NVGPTNLGGRTRAVMVDKNNSSIVYAAGVSGGLWKSTSGGTSWSPVNNTENMFISSLI
                     QDKDGYIYLGTGEYFASPLGDNNGGSGFRGNGIYKSTEPNGSEFQLLSATQAPNVSSP
                     FLFINQMAVNLTTNRIFAATHRGLQVSDDGGVSWYNPISGTLATQNCYDVEVANDGTV
                     YVAMSRYIYKSLNGDNASFEQLANPWGTNVARIEIAVMPTNSSIIYAVIINNNDGNLN
                     GAYRSIDGGANWELIGPGGSTTFYPFNQQGTYACALAVDPHDQDHILMGGIDLWEYYY
                     GGNWEKISLYYLDETSVLYLHSHQHKIVFDPNNPDVIYIGNDGGVFKGVRDATYDIFT
                     FTNANRKYVTSQFFTIGCNSYNQLMGGTYNNGTVLLPRQTTDSTKATKYLYGTGGYVA
                     MSNLDRKMIIGSYPYGELYRSNEFGGNSQTFYSSGVTAYIKSIGGPEAPGSDGFSSYL
                     APILLKEGYDFNTTDSITFINKNDTTLHIGDEIMVQNPVSGLYISDTLTIELEPGDSI
                     KVQDYLFANLFVGLRGSVWMTRGLHDYSAPPPWYKIASIPNNEYPQCMALSEDGDYLF
                     FGTTNGKVYRVSNLRQAKDSLSADDRSVYCVVACDQIYSISNRPITSISVNPNDPNKV
                     LITCGGYGNAIYVYYSINALTDNPTFNNKTGNLPKNPVYSSLIVKENPNVILLGTEYG
                     IYTTDNITISSPNWTKDNEGMGNVPVYMLTQQNFYTYNTPNYGVIYAATHGRGIFANY
                     KYRIVSIDDYNNNLSSFYVYPNPTTDFIYIINQDFNDPFAIYIYNMNGQLVFAKLVSE
                     DINSTIQIPVSSLNSGTYIIRCEGNNKVYSSKFIKE"
     gene            complement(7398..8255)
                     /gene="lipA"
                     /locus_tag="PKI65_03870"
     CDS             complement(7398..8255)
                     /gene="lipA"
                     /locus_tag="PKI65_03870"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOB77613.1"
                     /translation="MSEKGIKRKPDWLKVDRTPVDAYANLHLALSRKNLHTICESGMC
                     PNKAECWTQGTATFMILGDICTRNCKFCAVNTGKPLSPDVDEPYKILEVVKLLNLKHV
                     VLTSVTRDDLPDQGAEHWAKCIKLLKDANFTVEALVPDFDGDATLIKELAMAKPDIFS
                     HNLETVRNLTSHVRTRATYETSLKTLDIANSFGMLTKSGIMVGLGEKFDEVIEVMDDL
                     LKVNCKIITIGQYLQPSKNNFPVAEYIKPEIFDEYKNIALKKGFDYVESAPLVRSSYH
                     SEKILIKKY"
     gene            complement(8252..9016)
                     /locus_tag="PKI65_03875"
     CDS             complement(8252..9016)
                     /locus_tag="PKI65_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77614.1"
                     /translation="MKLKYVIFLILIIVSTSTLVAQEQIAIGSGFERGVLLKQYLSFG
                     VNIHSQGWGISMDIGKNANIYNNYGLLVEFVDVHSFKEIKLRREGILNAKSFVYGKLN
                     YIYFLRTGFKSASTLNFKPYWGGVSVGKFYSIGFNSCFTKPVYLQIQKYSSYYDWEIV
                     EERYQPEKHDLNDILGRGSFFSGFNKIKYYPGIYLQGGFSFDFGDTQDKIRRLDIGAS
                     LDLFIKEVPIMAYENNTFYFITFFLKYNIGKNYNAK"
     gene            9147..11768
                     /locus_tag="PKI65_03880"
     CDS             9147..11768
                     /locus_tag="PKI65_03880"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOB77615.1"
                     /translation="MKLAQHYKADIVENKWYKIWLEKKYFHSEPNEKEPYTIVIPPPN
                     ITGILHMGHVLNNTLQDILIRRARMLGYNACWVPGTDHASIATEARIVANLKSKGVDK
                     HSLTRDEFMKYAWEWKEKHGGIILEQLKKLGASCDWDRTTFTMDDHYYKSVIHVFVDL
                     YNKGLIYRSHRMVNWDPLALTALSDEEVIYKKTNSKLYYVKYFLTDDSGYLTIATTRP
                     ETILADTAVAVNPNDDRYKHLIGKYVYVPLVKREIPIIADQYVDPEFGTGCLKVTPAH
                     DLNDYEIGKKHNLEIIDIFTPDARLNENAKFLIGQDRFEARENIIPLLEMEGNLTKIE
                     DYENNVAYSERTDVPIEPRLSLQWFLKVEELSKPAMQVVLDGTIKFYPDKFINTYRHW
                     MENIKDWCISRQLWWGHRIPVWYLPNGEFVVAHNEEEAYTLALKKNPNLKKIELHQDD
                     DVLDTWFSSWIWPIAVFNGILQPNNEDINYYYPTNDLVTAPEILFFWVARMIMAGLEY
                     RKQVPFYNVYFTGIVRDKLGRKMSKSLGNSPDPLDLIDKYGADGVRMGMLFSSPLGND
                     LLFDEALCEQGRNFANKIWNALRLVKMWKIDPVKPTDTINVKSISWFNEQLKASIIEI
                     NHYFDEYRISDALKSVYKTIWDYFCSWYLELIKPNYGEPVDANTYNSTINFFKTLMQL
                     LHPFMPFITEEIWHLLQDNYNIDDTIMLTPWPTADSYDENIINDINFIFEFISTVRSL
                     KAQYNITKEDIEVYYNDTEYDKVIKDYEELILKMAQVKSINKNKQLKNTAEALVRTIT
                     ISIPITETNIEEEKEKILKELDYYKGFLANVNKKLKNENFLKKAPDKVIENEKKKQQD
                     TIKKIESLKKLLKKYEN"
     gene            complement(11863..12207)
                     /locus_tag="PKI65_03885"
     CDS             complement(11863..12207)
                     /locus_tag="PKI65_03885"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOB77616.1"
                     /translation="MSNDLEELYMKIWELVASIPYGRVTTYGAIATTIGSKASARFVG
                     IALSRCYLAPFYVPAHRVVNRNGLLTGRHHFPGNQMQELLENENVVIIDNQVVNFKEL
                     FWNPMIESSDKI"
     gene            complement(12220..12882)
                     /gene="trmB"
                     /locus_tag="PKI65_03890"
     CDS             complement(12220..12882)
                     /gene="trmB"
                     /locus_tag="PKI65_03890"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOB77617.1"
                     /translation="MARKKLQRFEDIKSFSFYLETGYFTLQKQAFPLKGKWRKDFFKN
                     YNPLIVELACGKAEYTVEMAQHFPNKNFIGVDIKGDRMWVGANKVKELELKNVAFLRV
                     PIGLIEHCFDENEVDEIWIIFPDPQPKSRVSKRLTSPRFINRYLKILKPRGLIHLKTD
                     DDDLFNYTLEIIKNFNFKLIDCNYDIYKERGDDIITQIQTFYEQIWLSQGKTIKYLAF
                     QI"
     gene            complement(12875..13864)
                     /locus_tag="PKI65_03895"
     CDS             complement(12875..13864)
                     /locus_tag="PKI65_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77618.1"
                     /translation="MEKLIESIKKQESIEIPYGKLILIYNEAKVHYDSMLIYPPYFSI
                     QFLPNEGLNPTDFDIPKSTEWGSFANINDEIKFIPDAELIEILNAPFYGLQPAPIKIQ
                     KTFRNEIEFKETLLQEQKIVKEKKVKKKKTKVNSDKPKDTKVKADIDKKEKKQPKKQK
                     LIIDNIDNIENDDESRKPITEKKKGKKNLVPLLIIILLILSGGGYYYYENYLSIKNND
                     FELLLDKKLDYAILSESLIELLNTQLKNDTLRYFLIVASEKNKKVAQKESAHYSSMGF
                     SPFILYDSLVKRYRVALDSFNCRDTILMKTKLDEIKARYRKDAWILKKRIGDG"
     gene            complement(13924..14085)
                     /gene="rpmH"
                     /locus_tag="PKI65_03900"
     CDS             complement(13924..14085)
                     /gene="rpmH"
                     /locus_tag="PKI65_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOB77619.1"
                     /translation="MKRTYQPSRKKRRNKHGFRKRMSTKEGKKILARRRQKGRNKLTV
                     SDEFKGKKK"
     gene            complement(14171..16780)
                     /gene="clpB"
                     /locus_tag="PKI65_03905"
     CDS             complement(14171..16780)
                     /gene="clpB"
                     /locus_tag="PKI65_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOB77620.1"
                     /translation="MNLNNFTVKAQGIIQDAFQIAFEKKHQVVEDVHLLYSLLKNDED
                     IIPYLLKKIGINPSQVMQRVEEEINKIPRVEGGEQYMSSTATKTLQQAISEQKQMGDD
                     FVAVEHILLGLLESNGSAERILKSLGANRRDLLTAIQDLRKGTKVTTQSGDENYNALN
                     KYARNLNEMATKGKLDPVIGRDEEIRRILQILNRRTKNNPILIGEAGVGKTAIVEGLA
                     YRIVNGDVPDNLKDKVVYSLDLGALIAGAKYQGEFEERLKAVIKEVVESDGKVILFID
                     EIHTLVGAGATQGAMDAANILKPALARGELRAIGATTLNEYQKYFEKDKALERRFQPV
                     FVDEPSPEDSISILRGLKERYENHHQVRIRDEAIVAAVELSHRYISDRHLPDKAIDLI
                     DEAAARIRLEMNSMPEEMDELSRRIRQLEIEREAIKRENEPEKLKAIDEELAELNEQY
                     SVLKTKWLQEKEIIEQIQQYKQKIENLKLEAEQAERNADYGRVAEIRYGLLREAEEKI
                     KELEDKLKEVQGDKPLISLEVNAEHIADIVAKWTGIPVTRLMQSEKEKLLNLENELHK
                     RVIGQDHAIEAVANAIRRSRSGLSDPKKPIGSFLFLGSTGVGKTELSRALAELLFNDE
                     NNMIRIDMSEYQEPHSISRLIGAPPGYVGYEESGQLTEAVRRKPYSVILLDEIEKAHR
                     DVFNILLQVLEDGRLTDNKGRVVNFKNTIIIMTSNLGADIIRENFDNMLPGEEEKTFE
                     RTQFQILELLRKTIRPEFLNRIDEVIVFKPLNKEEIKEIVKLQLNKVGEMLANNGYTF
                     TYTEDAVEHIAEEGYDPLMGARPIKRVIQKRVIDELSKMIIAEKIKSDQPIVLDVIDG
                     AYVFYNKTSKLEE"
     gene            complement(17466..18236)
                     /locus_tag="PKI65_03910"
     CDS             complement(17466..18236)
                     /locus_tag="PKI65_03910"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOB77621.1"
                     /translation="MEFPGWTQHDVDGSSTYGFSGISFTNAYYTGSFIAFNPANTNPS
                     MADQPAIQPHSGNRFGACFAATTPPNNDWLITPQTNVLSSGANVKFWVKSYTADYGLE
                     RYAVWVSTTTTDISAFTKISEGNYLTAPATWEEKTFDLSDYAGQQVYIGIQCVSSDAF
                     IFMIDDIVVSDGFVSINDIEINDINIYPNPASDFVTVSNAENAEITIINMLGQVVTSQ
                     FANSSCEIVNISNLTNGTYIIRVENDNKVITKKLNVFR"
     gene            18581..18820
                     /locus_tag="PKI65_03915"
     CDS             18581..18820
                     /locus_tag="PKI65_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOB77622.1"
                     /translation="MSQIEEKVKAIIVDKLGVSENEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFNLSIPDEDAEKITTVGEAIEYINQHIQK"
     gene            18834..20084
                     /gene="fabF"
                     /locus_tag="PKI65_03920"
     CDS             18834..20084
                     /gene="fabF"
                     /locus_tag="PKI65_03920"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOB77623.1"
                     /translation="MERRRVVITGVGAITPLGNNVNEFWNSLLEGISGADFITHFDAS
                     KFKTKFACEVKNFDPANYFDRKELRKYDLVTTYGIVAANQAIQDANIKIDNLDPYRVG
                     VIWSSGIGGIITFFQEVKNFVEGDGTPRFNPFFVPKMIIDITAGHISMIHGFKGPNYA
                     TVSACASSANAIVDAVLLIRHGYADVIVTGGCEAAITEPGMGGFNSMNAISVRNDDPK
                     TASRPFDKDRDGFVMGEGAAGLIIEDLEHAKKRGAKIYAEILGFGLSADAYHITAPHP
                     EGEGAKHAMSFTLQDASIEKEKIDHINTHGTATPAGDIAEAKAIVSVFGDHAQNISIN
                     STKSMHGHLLGAAGAIEALATILSIQNDAVPPTINHFTDDPDIPKLDFTFNHKKERIV
                     HYAISNTFGFGGHNVSLLFGKYSE"
     gene            20087..20815
                     /locus_tag="PKI65_03925"
     CDS             20087..20815
                     /locus_tag="PKI65_03925"
                     /inference="COORDINATES: protein motif:HMM:NF025973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOB77624.1"
                     /translation="MKSDKENNSSRLKYLKKLIKSILGFSPQNLYLYYLAITPRFVEA
                     TFNGERISNERLEFLGDAVLDLIVAQYLFHKFPHKDEGFLTEIRSRIVNTENLCKIAK
                     ETNLIEIVEKFLPTQHKTPPNNWLGNTLESFIGAIFLDKGYDFCYKFFISLIKDKIID
                     IEKIISEENNYKSRLLEYCQKNKLNLEFFSEKLQNNNFKIKILINNKLISEYIAPNIK
                     RAEKEASKIAYDKLINKKATIYVL"
     gene            21154..21621
                     /locus_tag="PKI65_03930"
     CDS             21154..21621
                     /locus_tag="PKI65_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOB77625.1"
                     /translation="MSKEIIVKSKSFIDGGMIPSKYTCDGANVSPQISWENIPEGTKS
                     LALICDDPDAPMGTFVHWVLYDIPPMIKELPENILPEKILPSGAKHGVNDFRKYGYGG
                     PCPPSGTHRYYFKLYALDTMLNLEPGKKKAELLKEIEGHIIAQGQIMGKYSRN"
     gene            21643..22221
                     /locus_tag="PKI65_03935"
     CDS             21643..22221
                     /locus_tag="PKI65_03935"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOB77626.1"
                     /translation="MHVNLAIFASGNGSNAENIYHYFDGNQNIFVSLLLTNNRRAGVI
                     DRFINFNVPIVVIDNILASDGSFLIRLCETFNINIIILAGYLRAIPADLIKKYPDKIL
                     NIHPALLPKYGGKGMYGSKVHENVIANNEKTSGISIHLVNENYDEGHIIFQKTISIDS
                     TDTPDTLAEKIHKLEYEYYPIVIEKYINELFT"
     gene            22253..23176
                     /gene="queG"
                     /locus_tag="PKI65_03940"
     CDS             22253..23176
                     /gene="queG"
                     /locus_tag="PKI65_03940"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOB77627.1"
                     /translation="MSNYLTDHEWQKIKQIFIKQSISEVGISESDEVPEIVKGNFLAY
                     LQSHYNGDMKYLERNLEKRFSPTTLYPGTQSIITFLLPYYQIFPAEWAEKISIYVTKK
                     DYHTLAKSISIPVVNFLKQLDANFRPRIFVDSAPVIDKYWAWKAGLGFIGKNTLLINE
                     KYGSFVFIVHIFTSLKAPPDALKNPLKNKCGNCTNCLKACPTQALISPKVLNASQCIS
                     YLTIEKSDNNSLHQVKKLVPYIYGCDICQKKCPYNNNPQITPIKDFLFDEQEKQELKN
                     WGTGISKFPLYLLNDSVLMKNVNFIKNKYKS"
     gene            complement(23243..24334)
                     /locus_tag="PKI65_03945"
     CDS             complement(23243..24334)
                     /locus_tag="PKI65_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOB77628.1"
                     /translation="MIVIGLTGTIGAGKDTVVDYLIKNENFEHFSVRKFLIKELKIRK
                     IPVNRDTMRQLANELREKNGASYIVEKLYSEAIKTKKNAIIESIRTVGEVEALKKNDN
                     FFLIAIDADPKIRYERIRQRGSETDDIDYATFIAQENEEMKNEDPNKQNISKCIELAD
                     YYIENNGTFQQLYDQVDAIMKEIKEKIESKEKGYIYQRPSWDEYFIEIMNTVATRATC
                     DRGRAGCVIVKDKRILVTGYVGAPSGLPHCDEVGHLFRKIIHEDGSITQHCVRTVHAE
                     QNAIAQAARFGISLEGSTLYVRMTPCRSCAMLIINSGIKRVVCEYRYHAGKDSEDLFK
                     EANIEIYFINDEILSYENQIINKKNEVND"
     gene            complement(24446..24820)
                     /locus_tag="PKI65_03950"
     CDS             complement(24446..24820)
                     /locus_tag="PKI65_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77629.1"
                     /translation="MTERCCLECGEPLVGRSDKKFCDDQCRNAYNNRLNRDSINIYRK
                     INNKLRKNRRIISELNKTGKTKIKIEALRKKGFDFKYHTHILNTAKGTTYFFCYDMGY
                     LIIDNEYALLVYDKEIKEDDNK"
     gene            complement(24825..25415)
                     /locus_tag="PKI65_03955"
     CDS             complement(24825..25415)
                     /locus_tag="PKI65_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77630.1"
                     /translation="MNIYRITQDNDTLIISYSPKEKLYRIYFNDDKIGDISKESMKNG
                     VDLDLENKKIKLSLNGKYSNRPFIIYNDKIYSGEQEAKKRYKINLSFFLLWFILELIG
                     AIWSYVNYNTVSFYIIFILVSSLVGIFICFYLKKNISHKVYLIIPLIFAMQIVTNISV
                     NMILTVFDLIFISVFLYCIWEYSFTKNFSKFVLKKS"
     gene            complement(25447..27081)
                     /locus_tag="PKI65_03960"
     CDS             complement(25447..27081)
                     /locus_tag="PKI65_03960"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOB77631.1"
                     /translation="MKQITEIAENLGIQADQLELYGKFKAKLPINLINEEKVKQSKLV
                     LVTAITPTPAGEGKTTTSISLSMALNKIGKKSVVALREPSLGPVFGIKGGATGGGLSQ
                     VLPTDDINLLFTGDIPAIERANNLLSALIDNNLQSKNRNLNLDSRTIHWNRVMDMNDR
                     ALRDIVIGLGGISNGIPRESSFEITAASEVMAILGMTDSISDLKERLGNIFVGLTWDK
                     KPIFARDLNAQGAMAALLKDAINPNLVQTTEGTPAIIHCGPFANIAQGTNSILATKMA
                     MTYSDVVITEAGFGSDLGAEKFFDIKCQRFGLMPNAVVIVVTIRALKYHGGASKDELF
                     IPNVDLVKKGLENLDKHIENMMMFNFKPIIALNKFDTDSEAEIDIVRQHCKEYGVLMS
                     INEGFNKGSEGAIDLAEKVIESTSYCPNCFKPIYTYDLSIEDKINTICKNIYGAKDVE
                     YSLKARKDLELIKMLNLENSPVCIAKTQYSLSDNPNLRGRPKDFILNIREIEISTGAG
                     FVVPIAGAIMRMPGLPEQPSAEKIDIDIDGKIHGIS"
     gene            complement(27122..28393)
                     /locus_tag="PKI65_03965"
     CDS             complement(27122..28393)
                     /locus_tag="PKI65_03965"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOB77632.1"
                     /translation="MIIRFLYTIGIYIYGFIINLGALLGHKKAKLWVKGRKYQKDIYK
                     KLSPTDRWIWVHVSSLGEFEQARTVIDGLHIKYSEHKILLTFFSPSGYEIRKNYPNVD
                     LVLYLPLDVPRNIKRFISTFLPELAIFVKYDFWFNYLYYLKRNDIPTILISANFRPNQ
                     YFFKWYGAYMRRYLKYFDHIFVQNNVSKLLLEKYMILNVTVAGDTRFDRVYEIANNPL
                     YIPNIETFIDNRFVYIAGSSWPEDEKYILHAINKYPEWCYIIAPHEVHEDRIRKLKSS
                     FNDDCITYSQLKSENSNFNDVKNKNILIIDIIGLLSHIYKYANIVHIGNGFGSGIHNI
                     LEAAIYGKPIIFGPNYKKFQEAIDLINLRGAFSFSNKEEYFDILNILYNDKIIYNNAC
                     EVSREYVKSNIGATKIILNYIDEKIKNYWQI"
     gene            28694..29596
                     /locus_tag="PKI65_03970"
     CDS             28694..29596
                     /locus_tag="PKI65_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77633.1"
                     /translation="MKKIRLLFVFTFISFLCFSQDSIKVKWRLSSIDFSFGYFNITNF
                     ETPTSWPPYIQYFKNIDAINDYYYDANNYWTSYINKKTDGSNIKFLLGFQLPKMQIKN
                     NREFLFGINFHQNVNFRHSLIVLERHPIDTFINVHSGQDLFFDSTHVISDDYNYSFNL
                     LDFDFGYNYMFFTGKRYQIHLAPYIGLGLTINRHAYMNRYLYDKYMFFLPEDSLFAQS
                     EIIKKYESLETIYDDNIKNLFYLKFGLPIRINLGLGNEDKNLDEISLFFEINPHYLYI
                     PSHDPVISSFTLSCSFGIKIKFLK"
BASE COUNT        10520 a         4592 c         3901 g        10911 t
ORIGIN      
        1 aaatttaata aattttctga tattaatcct gtataaattt taacagatta aatataaata
       61 cacacctacc ccttattata atgtaatgaa aaattgaatt tttaactgta attttgtcaa
      121 aaataataac taagattaat aatggtaaaa atcaatacaa aaaaattttt tgatcagata
      181 tcaccaaaaa gttattatga catagctatc attttgccat tattaatgtt atttatttat
      241 ccatctcgtc cagctatagg agtggcagga tttattttat ttacaatatt tggaataata
      301 aaaaaagatt taacttttca gtggaatgct aatattttat gggtaatatt ttttttatta
      361 tatgtaatat ttttttttaa aactacaaat aaacaagcag gactcttcga attagaaaaa
      421 aaactttcac tattggtctt tggtagtttg tttttattta aatataataa accgatttct
      481 attccatttt ggattatttc atttattatt ttgatattta taaggggcat tatcaatttt
      541 attgaagctt ttgtaatgta tagggatatt catctttttc aagctttttt aactactaga
      601 ttttcacaac acattcatcc tacatatttt agtatttaca ttattgtaag cttatttggg
      661 ctaatatatt tacaacataa aaggtttata aaattaccta aatggttgat aatttgttat
      721 ataatttttt ctatattatt aataatattt ttatcatcta cagctgctat tttatttttt
      781 ttattttcca tagtattttt ccttattttt tatttttata aaaaattaaa accgctcata
      841 gcaacaatat ttattataac tattttatta atttcattca taacaataaa atatatacca
      901 tttaaaacga taaatctcaa agaaacaata gaaaatacaa ttttattttt aaataatcca
      961 aaagaatgta ctcaatcaaa agaatttata gaaagtagca atgaaaaaag attgatttta
     1021 tggaaattaa gctgggaaaa atttaaggaa aatccattag gatatggagt aggaagtgct
     1081 ggagatgtat atagggatgt ttatcaaaaa tatgaattag actcatttat agagccgttg
     1141 cttgattctc ataatcaata tttgcgcata gctttagaaa tgggcattat aggcttatta
     1201 gcctatttga gcattttaat cacattcata gttaaagcaa ttaaacaaaa aaaatattta
     1261 ctcttatttg ttttgattag cttgatttat tttggattat tcgaatcagt tctacaacgt
     1321 caatcaggtg taatattttt tgtctatatt atcacatttt tatatttata tgaaccaaat
     1381 ttattagatt ataataaaga aacataaatt tatgaaaaaa atacttgtag tcagttctaa
     1441 ttctatacat ttatttaatt tttataattt aataaattca tatttcgacc acatagatat
     1501 tgttacagat agtattaatg aaaaatataa ttatcgcaat attgtattgt acaaatataa
     1561 tttttccata aagaaaccat ttcaatttat aatttcttat ttcaaattaa aaaaattaat
     1621 aaaaacaaat aaatatagta ttattcatat tcagcagatc acaactgccg ctctattagc
     1681 aatattagct tcaaaaaaat caaaaattcc aatagtagcg actgcatggg gtagcgatat
     1741 tttattaatt tctcacagag gatattttta taaaaaatta gtaaaatatg tattaaaaaa
     1801 tgttgacata gtgactgctg actcagaatt tgtaatacaa gaaatgaaca aattaactaa
     1861 acaaaatata gatgcacatc tcgtaatttt gaccaattta cattctactc ctaaatactt
     1921 cccaaaaaaa tcaaaaatca tttattctaa tagattacta actccacttt acaggataga
     1981 tctgataata aatgcctttg aaaaatttat aaaagataaa caatacgaag aatggaaatt
     2041 aataatagct ggtcagggaa atgaaagaca taaattagaa aaaatagtta gagaaaaaaa
     2101 attacaaaac caaatagaat ttgtaggctg gcaatcaacc gataagaata atgagtatta
     2161 ccaaaaagct cttatctaca tttctatacc gatctctgat ggcactccta cgagtttatt
     2221 agaagctatg agctatggtt gcattccgat agtgtcagac ttacctgtat actatgaatg
     2281 gattgaaaac aatgtaaatg gattgatagt taatgatttg aactctaatt ttatagaaaa
     2341 agccttaaaa ttaaatccaa ttaaagtttg ggaaattaat cgtaatatta ttgaaaaaaa
     2401 agcaacaatc gacgttaata aatctaaatt ggagcaaata tataataaat tattacaaac
     2461 taaataaata cccaaaacaa tttttataaa aatttacaac tcacttaact cgtaaactcc
     2521 tacacctctt acatgctcaa ctccactttc tctacttttt acactctcca aattccttca
     2581 ttgttttctt tacattgata ttagaattta tttaataatt aattaacata attttagtaa
     2641 aacatcatta cttctataac gtttgttgtg ggtaaaaaca ttttagattg ttacaaaaaa
     2701 ataactattt tttaattatt taggttgttt attttaccac aaagaacacc aagttattac
     2761 acaaaatcca caaattacca tttgtaggcc ttttaattcc atccttctct ttgtgcattc
     2821 tttgagaact ttgtggttga tatttaactg tctaataata aataaaccac tattgacaaa
     2881 ttcatttttg taaaattaca cttttttttg atctttacct atataacttt acctatataa
     2941 aacgttatag aacgacatta ttaatcaact tctaaaaaaa gtaacatcta tttttttaat
     3001 ttgttaattt ttgcacaatt aactttaaat aaatcttttg ctcagttgta tttattttga
     3061 tttagataat aaatcttgct aaaaagtaca gatctaatta atgctttaca accaatttct
     3121 tagcaaaata tacatcaata ccattaaatt ctattatata aataccatta tccaaactac
     3181 ttattggtat tgtagtatat acagctttgt ttattttttc tctatatact tcttgcccta
     3241 agatattttt gattactatt gcaccagttt gttcagaatt aaaattaact tttacataat
     3301 cactagctgg atttggcaat atagtgaaat tagaactttg gtaattattt atgctagtat
     3361 tatcaatagt ttcaaatgac catgtatcag acattaattc atctctaacg tgcttagctt
     3421 tgatagccca ataatatgtt ttattatatt ttagatttag atttcgtaaa taataaacaa
     3481 atttatcttt tcgattatat aaggtatctt gagagttaat agatacagtt ttactagtag
     3541 ataaattact taatgtatca taataaatta tatattcatc agctgcatat atactggacc
     3601 atgaaagtga atcactggat ggatttatat cttgagctcc attacttggt aaaagcaaac
     3661 caagtgtatc aatagttttg aaatggtata cgtcgctcca atcagatgta tcttttgcat
     3721 gaaatagtct tgccttccaa tagcaatctt ggttaaattt caagtctcct gttgctactt
     3781 tagtcaatgt atcatactta ggcggattat tacttacttt tattctagct attttagtgc
     3841 tatcaaatat gtaataaaat tgaacgtcaa aactgctatc ttctgatatt attaattctc
     3901 catattgact tccaaaaata gtatgaaaaa ttatactatc accaggaata aacccttcaa
     3961 agttatcagt aggatattta gctattacat cccctttagt tgtaaaaaat ctaacatcag
     4021 accattctga actatcatag tcatggtacg cttttactct ccaataataa ttttgaccat
     4081 agtttaattg aaataaatca atagtatcaa tattcaattc actatactct ttctttaata
     4141 aactattaaa atctgatgta gtatctaatt gaaataaata tttagaacct tttatagcat
     4201 tccacctaaa agtaaatttt acaggtaatt ttttagaatt atctgcagga atagttaaag
     4261 taactgaagc tttagtagta aaattccaaa cttcactcgg gctagaatac agattattaa
     4321 catttttagc acttacacgc caataataag tagtaccata taacaaatct tttactgtat
     4381 gtgccgagta tgtagtttca attgtctgtt ttaatggaga actaaaatta tttgaagtat
     4441 ctatttccaa cttataagac actgcaccgc taacgctaga ccagtctaaa ataacttcta
     4501 catctatatt tgaactatca gctggtgaaa ctaatgtagg agcagcaatt ttatcctgtg
     4561 ctgcaatatt aactaaaatt cccaggaata acaatccaaa aactaaaatt tttctcataa
     4621 ttatatattt ttattctttt ataaatttag aactataaac tttattattg ccttcgcatc
     4681 ttataatata tgtaccgcta ttcaaagaac tgacaggtat ttgaatagta ctattaatat
     4741 cttctgatac caattttgca aaaaccagtt gtccattcat attataaata tatatagcaa
     4801 aagggtcatt aaaatcttga tttatgatat aaataaaatc agtagttggg ttaggataca
     4861 catagaagct acttagatta ttgttataat catcaataga gactattcga tatttgtaat
     4921 tagcaaatat acctctacca tgcgtagcag catatataac accataatta ggcgtgttat
     4981 aagtataaaa attttgttgt gtcaacatat atactggtac atttcccatt ccttcgttgt
     5041 ctttagtcca attaggagat gatatagtta tattatcagt agtatatata ccatactctg
     5101 tacctaataa aataacatta ggattttctt ttactatcaa cgaagagtat acaggatttt
     5161 taggtaaatt cccagtttta ttattaaaag ttggattatc agttagtgca ttaatagaat
     5221 aataaacata aatagcatta ccataaccac cacatgtaat taatacttta ttgggatcat
     5281 taggatttac tgaaatagaa gttattggtc tattagaaat gctataaatt tgatcacaag
     5341 ctactacaca atatacacta cgatcatcag ctgataagct atcttttgct tgacgtaaat
     5401 tagacactct ataaactttg ccattagtag taccaaaaaa taagtaatca ccatcctcac
     5461 tcaaagccat acattgtgga tattcattgt ttgggatact tgctatttta taccatggag
     5521 gtggtgcaga ataatcatga agtcctctag tcatccaaac tgaacccctt aacccaacaa
     5581 ataaattagc aaaaagataa tcttgtactt ttattgaatc accaggttct aactcgatag
     5641 ttagagtatc tgaaatataa agtccactaa cggggttttg aaccataatt tcatcaccaa
     5701 tgtgcaaagt agtatcattt ttattaataa aagtaatact atcagttgta ttaaagtcat
     5761 agccttcttt taaaagtata ggagctaaat aagaagaaaa accatcagag ccaggggcct
     5821 caggaccacc aatactttta atataagctg taactccact tgaataaaat gtttgactat
     5881 taccaccaaa ctcattagat ctatatagct caccataagg ataagaacct ataatcattt
     5941 ttctatcaag gttagacata gctacgtagc caccagtacc atataagtat ttagtagctt
     6001 tagtagaatc tgttgtttga cgaggcaata ataccgttcc attattataa gtaccgccca
     6061 tcagttgatt ataagaatta catccaatag taaaaaattg agaagttaca tattttctat
     6121 tagcattagt aaaagtaaag atatcataag tagcatctct tacaccttta aacacacctc
     6181 catcattgcc tatatagatt acatctggat tatttggatc aaaaacaatt ttgtgttgat
     6241 gagaatgtaa gtacaaaaca cttgtttcat ctaaataata caaagatatt ttttcccaat
     6301 tccctccata ataatattcc cataaatcaa tgccacccat taatatatga tcttgatcat
     6361 gtggatccac tgctaaagca caagcatatg ttccttgttg attgaaaggg taaaatgttg
     6421 tactgccccc aggaccgatt aattcccaat tggcaccccc atcaatcgat ctatatgcac
     6481 catttaaatt accatcatta ttatttatga ttactgcata tataatagat gaattagtag
     6541 gcatcactgc tatctcaata cgagcaacgt ttgtgcccca cggattggct aattgttcaa
     6601 aagaagcatt atcaccattt aatgatttat aaatatatct agacatagct acataaacag
     6661 taccatcatt agctacctct acatcataac aattttgagt tgctaaagta ccagagatag
     6721 gattgtacca acttacgcca ccatcatcag atacttgtaa acctctatgt gttgcagcaa
     6781 aaattctatt agttgtcaga ttaactgcca tttgattaat aaataaaaat ggtgatgata
     6841 catttggagc ttgtgtagct gatagtaact gaaattcact accattgggc tcggtacttt
     6901 tgtatatgcc attgcctcta aatccagagc caccgttatt atctcctaga gggcttgcaa
     6961 aatattcacc tgtacctaaa taaatatatc catctttgtc ttgaatcaaa gatgatataa
     7021 acatattttc agtattgtta acaggtgacc acgaagtacc gccagaggta ctcttccata
     7081 aaccaccaga aactcctgca gcataaacta tagaagaatt atttttgtca accattacag
     7141 ctctagtacg tccacctaga ttagtaggtc ctacatttac ccactgtagt ccctgagatt
     7201 ttaatggcgt gtttttctga ttatttttca cgttgttttg ctgggcatta ccacttataa
     7261 ataatgctat aactaacatt gcacttaata aaaatttgag tcttcgcata aatatatttt
     7321 ttaaatatta aactcttatg aaattttcag ccaaattaat aataattatt tatataaaac
     7381 aaaatttttt ttcaaaatta atattttttt attaatattt tttcggaatg ataagatgat
     7441 ctcactaatg gtgcagactc tacgtaatca aacccttttt ttaatgctat atttttatat
     7501 tcatcaaata tttctggttt tatatattct gctactggaa aattattttt agaaggttgt
     7561 aaatattgtc caatagttat gatcttgcaa ttaactttta ataaatcatc cattacctct
     7621 atcacttcat caaatttttc tcccaatcct accataatgc cagattttgt tagcatgcca
     7681 aaagaatttg ctatatccaa agtttttaaa ctagtttcat aagtagctcg agtacgtaca
     7741 tgactagtta gattacgaac ggtttctaaa ttatgagaaa aaatatctgg ttttgccata
     7801 gctagttctt ttattaatgt agcatcgcca tcaaaatctg ggaccaatgc ttctacagta
     7861 aaattagcat cttttaataa tttgatgcat ttggcccaat gctcagcacc ctggtcaggc
     7921 aaatcatctc gtgtaactga tgtaagaaca acatgtttta agtttaatag ttttactact
     7981 tctaaaattt tataaggttc atctacatct ggcgaaagtg gttttccagt attcacagca
     8041 caaaacttac aatttctagt gcatatatct cccaaaatca taaatgtagc tgtcccttgt
     8101 gtccagcatt cagccttatt tgggcacata ccactttcac aaatagtgtg tagatttttt
     8161 ctactcagag ctaaatgcaa atttgcataa gcatctactg gcgttctgtc tacttttagc
     8221 caatctggtt ttctttttat tcctttttca ctcatttagc attataattt ttaccaatat
     8281 tatattttaa aaaaaacgtt ataaaataaa aagtattatt ttcgtatgcc attataggta
     8341 cttcttttat aaataaatct aaagaagcac ctatgtctaa gcgcctaatt ttatcctggg
     8401 tatcaccaaa atcaaatgaa aatccacctt gtaaatatat accggggtaa tattttattt
     8461 tgttgaaacc agaaaaaaaa cttccccttc ccaatatatc gttcaaatca tgtttttctg
     8521 gttgatatct ctcttctact atttcccaat cataatatga actgtatttt tgtatttgta
     8581 aatacacagg cttagtaaaa caactattaa aaccaataga ataaaatttg cctactgata
     8641 ctccacccca ataaggttta aaattcaagg tagatgcaga tttaaagcct gtacgcaaaa
     8701 aataaatata atttaattta ccatatacaa atgattttgc atttaatatt ccttctcttc
     8761 gaagtttaat ttctttaaaa gaatgtacgt ctacaaattc tacaagcaac ccataattat
     8821 tataaatatt agcattttta cctatatcca tagatatccc ccaaccttga gagtgtatat
     8881 ttaccccaaa actaagatac tgctttaata gcacacctct ttcgaaacca ctaccaatgg
     8941 ctatttgctc ttgagctaca agggtagagg tagatactat tatcaaaatt aaaaaaataa
     9001 catattttaa tttcatatct acaaaattaa ataatttttt attattttgc ttaattataa
     9061 aatgaattta taaagagaca attagtaagt tattttttta aaacaaattc aatttttcct
     9121 tgtatttttg cataaaatct aattttatga aacttgctca acattacaaa gcagatatag
     9181 tagaaaataa atggtataaa atatggctcg aaaaaaaata ttttcattca gaaccaaatg
     9241 aaaaggaacc atatactatt gtaataccac caccaaatat cactggtatc ttgcatatgg
     9301 gacatgtatt aaacaatact ttgcaagata ttttgatacg ccgtgcaagg atgctaggct
     9361 ataatgcttg ttgggtacca ggcacagatc acgcatctat agctacagag gcccgcatag
     9421 tggctaatct aaaatctaaa ggtgtagata agcattcgct cactcgtgat gaatttatga
     9481 aatatgcctg ggaatggaaa gaaaaacatg gtgggataat actggaacaa ttaaaaaaat
     9541 taggtgcttc atgtgattgg gacagaacta cctttacaat ggatgaccac tattacaaaa
     9601 gtgtaataca tgtatttgta gacttataca acaaaggcct tatttaccgc agtcacagaa
     9661 tggtaaattg ggacccattg gctctaactg ctctttctga tgaggaagta atttacaaaa
     9721 aaactaatag caaactttat tatgtaaaat actttctcac tgatgatagt gggtatctga
     9781 caatagctac aacacgtcca gaaactatac tggcagatac agccgtagca gtcaatccta
     9841 atgatgatag atataaacat ctaattggca aatatgtgta tgtgccatta gtaaaaagag
     9901 aaattcctat aatagcagat caatacgtcg atccagaatt tggcacaggc tgtctaaagg
     9961 ttaccccagc tcacgattta aatgattatg aaataggaaa aaaacataat ttagaaatta
    10021 tagatatttt cactcctgat gctcgtttaa acgaaaatgc aaaattttta ataggtcaag
    10081 atagattcga agctcgggaa aatataatac cacttttaga aatggaaggt aatttaacta
    10141 aaatagaaga ctatgaaaat aatgtagcat atagtgaaag aactgatgtg ccaatagaac
    10201 cacggcttag tttacagtgg tttctaaaag tcgaagagtt atcaaaacct gcaatgcagg
    10261 tagtattaga tggtactata aaattttatc cagataaatt tattaatact taccgccatt
    10321 ggatggaaaa tataaaggat tggtgcatat ctcgtcagct ttggtgggga catcgtattc
    10381 cagtatggta tttgccaaat ggtgaatttg tagtagctca taatgaggaa gaagcttata
    10441 ctttggcatt aaagaaaaat cctaatctca aaaaaattga attacatcaa gatgatgatg
    10501 tactagatac ttggttctcc agttggatat ggccaatagc agtatttaat ggaattctgc
    10561 aaccaaataa tgaggacatt aattattatt atcctactaa tgatttggta acagctcctg
    10621 agattctatt tttttgggta gctagaatga taatggctgg actcgaatat cgcaaacaag
    10681 tgccttttta taatgtatat tttacaggta tagtacgaga taaattagga cgcaaaatgt
    10741 ctaaatctct cggtaatagt cccgatccat tagatttgat tgataaatat ggagcagatg
    10801 gcgtaagaat gggaatgctg ttttctagtc ccttaggtaa tgatttactt ttcgatgaag
    10861 ctctttgcga acaagggaga aattttgcta ataaaatatg gaatgctctg cgcctagtaa
    10921 aaatgtggaa aatcgatcca gtaaaaccta cagatactat taatgtaaaa agtatttctt
    10981 ggtttaatga acaattaaaa gcctcaatta tagaaataaa tcattatttc gacgaataca
    11041 ggatatctga tgcgttaaaa tcagtttata aaacaatatg ggactacttt tgctcttggt
    11101 atttagaatt gatcaaacca aattatggtg aaccagtgga tgctaatact tataatagta
    11161 caataaattt tttcaaaact ttaatgcaat tacttcatcc ttttatgcct tttattaccg
    11221 aagaaatatg gcatttatta caagataatt ataatataga tgatactata atgctaacac
    11281 catggccaac agctgatagt tatgatgaaa atattataaa tgatattaat tttatttttg
    11341 aattcatttc tactgttcgc tctctaaaag ctcaatataa tataacaaaa gaagatatag
    11401 aagtttatta taatgacact gaatatgata aagtgataaa agattatgaa gaattaatac
    11461 taaaaatggc acaagtaaaa tctataaata aaaataaaca attaaaaaat acagccgaag
    11521 cattggtgcg tactattact atatctattc ctataactga aacaaatata gaagaagaaa
    11581 aagaaaaaat tttaaaggaa ttagattact ataaaggttt tttagccaat gttaataaaa
    11641 agctaaagaa tgaaaatttt ttaaagaaag ctcccgataa agtgatagaa aacgaaaaga
    11701 aaaaacagca agatactata aaaaaaatag aaagtttaaa aaaacttttg aaaaaatatg
    11761 agaactaaaa aatatatatt tgttaaatta ttatttttaa caaatatatg acaaattaaa
    11821 aattctcaaa atcttcgttt tttagttttt tgctaaagat gtctaaattt tatcacttga
    11881 ttctatcatt ggattccaaa ataattcttt aaaatttact acttgattat caataattac
    11941 aacattttca ttttctaaaa gttcttgcat ttgattacca ggaaaatgat ggcggccagt
    12001 aaggagtcca ttacgattga ctacacggtg agcaggcacg taaaaaggag ctaaataaca
    12061 acgagaaaga gcaataccta caaagcgagc cgaggctttg cttcctatcg tagtagctat
    12121 agctccataa gtagttaccc tgccatatgg tatactagca accaattccc atattttcat
    12181 atataactct tctaagtcat tactcataac aaaaaaatat taaatttgaa atgctaaata
    12241 tttaattgtt ttgccttgtg atagccatat ttgttcgtaa aatgtttgta tttgagttat
    12301 aatatcatcg cccctttctt tgtaaatatc atagttgcaa tcaataagtt taaaattaaa
    12361 atttttgatt atttctagtg tataattgaa aagatcatca tcatcagttt ttaaatgaat
    12421 gagtccacga ggttttagaa tttttaaata tctattaata aaacgagggg aggtgaggcg
    12481 tttacttaca cgactcttgg gttgtggatc tggaaaaata atccaaattt catcaacttc
    12541 attttcatca aagcagtgtt ctattagtcc aataggtaca cgcaaaaaag caacattttt
    12601 taattcaagc tctttaactt tattagcccc tacccacatt ctatcacctt taatgtctac
    12661 accaatgaaa tttttatttg ggaaatgctg agccatctct acagtatatt cagctttacc
    12721 acaagctaat tcaactatta atgggttata atttttaaaa aaatcctttc tccatttacc
    12781 ttttagagga aaagcttgct tctgcaatgt gaaatatccg gtttctaaat aaaatgaaaa
    12841 agatttaata tcttcgaagc gttgcagctt ttttctagcc atcaccaata cgttttttta
    12901 gaatccacgc atcttttcta tatctagctt ttatttcgtc taatttagtt ttcatcaaaa
    12961 ttgtatctct gcaattaaat gaatctaaag ctacacgata tctttttact aaactatcat
    13021 ataaaataaa aggcgagaat cccatagagc tataatgagc actttctttt tgagctactt
    13081 ttttattttt ttcactagct actatcaaaa aatatcttaa tgtatcattt ttgagctggg
    13141 tattcaataa ttctatcaat gattctgaga gtatagcata gtctaatttt ttatctaata
    13201 ataattcaaa atcattattt tttattgata aataattttc ataataataa tagcctccac
    13261 cagataaaat cagtaaaata attatcaaaa gagggacaag attttttttg cctttctttt
    13321 tttcagtaat aggcttacgt gactcatcat cattttcaat attatcaata ttatcaataa
    13381 ttagtttttg tttttttggt tgtttctttt cctttttatc tatatcagct tttactttcg
    13441 tatcttttgg tttatcagaa tttacttttg tttttttctt ttttactttt ttttctttta
    13501 cgattttttg ttcttgtaac agagtttctt taaattctat ttcattccta aaagtttttt
    13561 gtatttttat tggagcaggt tgcagtccat aaaaaggagc atttaaaatt tctatcaatt
    13621 cagcatcagg tataaatttg atttcatcat ttatattagc gaaactcccc cattctgtag
    13681 acttgggaat atcaaaatca gttgggttta atccttcatt gggtaaaaat tgaatagaga
    13741 aataaggtgg ataaattaac atcgaatcat aatgaacttt tgcttcatta taaataagaa
    13801 taagcttacc ataaggtatt tctatgcttt cttgcttttt aatagattct atgagctttt
    13861 ccattgtaaa aaacaaaaca aagacctatt aataggtctt tgtttattat acaattaata
    13921 tatttatttt ttcttacctt taaattcatc agaaactgta agcttgttac gtcctttttg
    13981 cctacgacgt gctaatattt ttttaccttc tttggtagac attctttttc taaatccgtg
    14041 tttatttcta cgtttctttc tagatggttg atatgttctt ttcatatttc aactatttga
    14101 aatgcaaagt tataaaaatt tttttaatta ataattttta taacttattt tattttcaaa
    14161 ttaatattaa ttattcttca agttttgagg ttttattgta aaagacataa gctccatcta
    14221 ttacatctag tactatgggt tgatcagatt ttattttttc ggcaattatc attttagaga
    14281 gttcgtctat tacgcgtttt tgtatcactc ttttaatagg tctagcgccc attagagggt
    14341 cgtaaccttc ttcagcgata tgttctactg cgtcttcggt atatgtaaaa gtatatccgt
    14401 tattagctaa catttctcct actttgttaa gttgtaattt tacaatttct tttatttctt
    14461 ctttatttaa tggtttaaaa acaataacct catcaattct atttaaaaat tcaggtctaa
    14521 tagttttacg caacaattct aatatttgaa attgtgttct ttcgaatgtt ttttcttctt
    14581 caccaggaag catattatca aagttttctc ttatgatatc agcacccaga ttagaagtca
    14641 tgataataat agtattttta aaattaacaa ctctaccctt attatcagtc aatctaccat
    14701 cttctaatac ttgcaaaaga atattgaaaa catcacgatg agctttttct atttcatcta
    14761 atagaataac actataaggc tttctgcgta cagcttctgt tagttgccca ctttcttcat
    14821 aaccaacata ccctggtgga gctcctatca atctagaaat actgtgtggt tcttggtatt
    14881 ctgacatatc tattctgatc atattatttt catcattaaa taataattca gctagagccc
    14941 tagatagttc tgttttacca actccagtgg atcctaaaaa tagaaatgat ccaataggct
    15001 ttttaggatc gctaagccca ctacgacttc ttctaatagc attagctact gcctcgatag
    15061 catgatcttg acctattact cttttgtgca actcattttc taaatttaat aatttttctt
    15121 tttcgctttg cattaatcga gttacaggta taccagtcca tttagctaca atgtcagcaa
    15181 tatgctctgc attaacttct aaagaaatca atggtttatc accttgcact tctttgagtt
    15241 tgtcttcgag ttcttttatt ttttcttcag cttctctcaa aagaccatac ctgatctcag
    15301 ctactcgacc ataatcggca tttcgttcag cttgctcagc ttctaatttg agattttcta
    15361 tcttttgctt atactgctgt atttgttcta tgatttcttt ttcttgcagc catttagtct
    15421 ttaggactga atattgctca ttaagttcag ctaattcttc atcaatagct tttagttttt
    15481 caggttcgtt ttctcgttta atagcttccc gctctatttc taattgccta atacgtctgc
    15541 ttagctcatc catctcttca ggcatagaat tcatttcgag cctaatacga gctgcagcct
    15601 catcaatcaa atctatagct ttatctggta gatgtctatc tgagatataa cgatgtgaaa
    15661 gctcaactgc tgctacaata gcttcatctc ttatacgcac ttgatgatga ttttcatacc
    15721 tttcttttaa ccctcttaat atactaatgc tatcctctgg tgatggttca tctacaaata
    15781 ctggttgaaa tctacgttcc aatgctttat ctttttcgaa atatttttga tattcgttaa
    15841 gtgtagtggc acctatagca cgtaattcac cgcgggctag agctggttta agaatattag
    15901 cagcatccat agctccttga gtagctccag cacctactaa agtatgtatt tcatcaataa
    15961 ataaaataac tttaccatca ctttctacta cttcttttat aacagctttg agacgttcct
    16021 caaattcgcc ttgatacttg gctcctgcta ttaaagcccc taaatctaat gaatacacaa
    16081 ctttgtcttt cagattatct ggtacatccc catttactat tctataagct aatccttcga
    16141 ctattgcagt tttacctact ccggcttcac ctattagaat gggattgttt ttagttctac
    16201 gattaagtat ttgtaatatc ctccttatct cctcatctct acctattact ggatccaatt
    16261 tccctttggt agccatttca ttaagattac gagcatattt atttaaagca ttataatttt
    16321 cgtcaccact ttgagtagtt actttagtac cttttcttaa gtcttgaata gctgtcaaaa
    16381 ggtctctacg attagctcct agacttttta atatacgttc agcacttcca ttactttcta
    16441 acaaacccaa caagatgtgt tctacagcta caaaatcatc acccatttgt ttttgttcag
    16501 aaatagcttg ttgtaatgtc tttgttgcgg tactagacat atattgttcg ccaccttcta
    16561 ctcgaggtat tttatttatc tcctcttcta cacgttgcat tacttgtgaa gggttaatac
    16621 ctattttctt caataaatat gggataatat cctcatcatt ttttagcaaa gaatataaaa
    16681 gatgcacatc ttctactact tggtgtttct tttcaaaagc tatttgaaag gcatcttgta
    16741 taattccttg tgcctttact gtgaaattat ttaaattcat aattattcct cctattttac
    16801 tacaaatata ttagcaattt ttaatccaaa ttaaaaataa tgataaaatt tcattaatat
    16861 tattcaaaca tttttataaa tttaataaat taaaacatat aatatgtttt ttaaaataaa
    16921 ataatgacaa tatgacataa aactaaaaat ttttcaatta ttctaaacat atattattta
    16981 aaatataata ttattaatgc agttttataa attagagagt agcatatatg gtgtgtagcg
    17041 tgattggttt atttacttaa tttttgttta aatctttatt ggttaaaata atattatcat
    17101 tatgcgcaaa attgtttctt aagtacttgc aaagctatat aatgcagtat gttgaaacgc
    17161 ataaatatct actaaaaaaa ttgatcaaag atactggaag gttgttaaat atagcgtcgt
    17221 caggagctaa acaaatatta attttaacat aaattatttt ttatatatct cctgcttcgt
    17281 ttaatatcta tacataatct aatcaactat acattattat ttagcataca gcatactttg
    17341 ttttttatat ataaacaaaa taaattaata tttgtttata tatcatttat tatttaatca
    17401 ctatttatta aaaaaagccc tgaaatattt cagggctttt ttatatgtat taagaaatac
    17461 tttttttatc taaatacatt tagttttttg gtaattactt tattgtcatt ttctacacgt
    17521 ataatataag ttccattagt taaatttgaa atattaacaa tttcacaact gctattagca
    17581 aattgagaag taacaacctg tcccagcatg tttataatag ttatctcagc attttctgca
    17641 tttgaaacag tgacaaaatc agatgcagga tttggatata tgttaatatc attaatttct
    17701 atatcattga tactaacaaa tccatcacta acaacaatat cgtcaatcat aaagataaaa
    17761 gcatcacttg aaacacattg tatcccaata taaacttgct gacctgcata atctgataga
    17821 tcaaaagttt tttcttccca cgtagccggt gctgtaagat aatttccttc agatattttt
    17881 gtaaaagccg aaatgtctgt tgttgtcgtt gaaacccaca cagcatagcg ctctaatcca
    17941 taatcagcgg tgtatgattt tacccaaaac ttaacattag cacctgaact taaaacattt
    18001 gtttgtggtg ttatcaacca atcattgtta ggtggggttg ttgctgcaaa acatgcacca
    18061 aaacgattac cactatgtgg ctgaattgct ggctgatctg ccatagatgg atttgtattt
    18121 gcaggattaa atgctataaa tgaacctgta taataagcat ttgtgaaaga gatgccacta
    18181 aatccataag tcgaactacc atctacatca tgttgtgtcc aacctggaaa ctccaagtca
    18241 aaatcattaa catttgtttc aaatccacca tccattatac ctgtgataga ttttattgct
    18301 gtttcagata aagaattttg gacttccgta ggaaatccga gatttttgac tttttcgttt
    18361 tgtgcaaata caaaagatat acttgctaca aacattaaac ctaataaaat tgtaaacttt
    18421 ttcataacat ttgattttta ggggttaagg gttaataatt tattttttac aaatttacat
    18481 atttttttca attaaacaaa ttaattttaa aaaaaatttt aatttttcaa ttaaaaaaaa
    18541 ttaattattt ttgccaaacc aaatttaaaa aaataaaact atgagtcaaa ttgaagaaaa
    18601 agtaaaagct attatcgtag acaaattagg tgttagtgaa aatgaagtaa caccagaagc
    18661 aagttttact aatgatttag gtgcagattc attagacact gttgagttga ttatggaatt
    18721 cgaaaaagaa tttaatctta gcataccaga tgaagacgct gaaaaaatta ctacagtcgg
    18781 agaagctatc gaatacataa atcaacacat acaaaagtaa aaatcttaaa gtaatggagc
    18841 gtagacgtgt agtcataaca ggagtcggtg ctattactcc tttgggtaat aatgtaaatg
    18901 aattttggaa ttcattactt gaaggaataa gtggtgccga cttcattact cactttgatg
    18961 catctaaatt taaaacaaag tttgcttgtg aagtaaaaaa cttcgatcca gctaattatt
    19021 ttgatagaaa agaattacgt aaatatgatc tagtcactac ttatggcata gtagcagcta
    19081 accaagccat tcaggatgct aatatcaaaa tagataactt agatccttat cgcgtgggtg
    19141 taatatggag tagtggtatt ggtggaatta taaccttttt tcaggaggta aaaaattttg
    19201 tagaaggaga tggtacacca cgtttcaatc ctttttttgt tcctaaaatg attatagata
    19261 tcacagcagg acatattagt atgatacatg gattcaaagg acctaattat gctacagttt
    19321 cagcttgtgc ctcttctgcc aatgctatag tagatgcagt attattaatt cgtcatgggt
    19381 atgccgatgt aatagtgact ggtggttgtg aagctgcaat tactgagccc ggaatgggtg
    19441 gatttaattc tatgaatgct atttccgtca ggaatgatga tcctaaaact gcttctagac
    19501 catttgataa agatcgtgat ggattcgtta tgggcgaagg tgctgcaggt ctcataatag
    19561 aagatctaga acatgctaaa aaacgcggtg ctaaaattta tgccgaaatt ttaggttttg
    19621 gacttagtgc agatgcttac catatcacag caccacatcc cgaaggcgaa ggcgctaaac
    19681 atgctatgag tttcactctt caagatgcta gtatcgaaaa agaaaaaata gatcatatca
    19741 atactcatgg taccgccact ccagcaggag atatagccga agctaaagct atcgttagtg
    19801 tatttggtga tcatgctcaa aatatttcta ttaactctac aaaatctatg catggacatt
    19861 tattaggtgc ggctggtgct atagaagctc tggctactat cttaagcatt caaaatgatg
    19921 ctgtgccgcc tactatcaat catttcactg atgatccaga catacctaaa ttagatttta
    19981 cttttaatca taaaaaagaa cgtatcgtcc attatgctat tagtaatact tttggctttg
    20041 ggggacataa tgtatctctg ctatttggta aatattctga ataaatgtga aatctgataa
    20101 agaaaataat agtagtagat taaaatattt aaaaaaatta attaagtcta ttctaggttt
    20161 ttcgcctcaa aatttatatt tatactattt agctataact ccacgttttg tagaagctac
    20221 ctttaatgga gaaagaatat ctaatgaaag attagaattt ttaggtgatg ctgttttaga
    20281 tttaatagta gcacaatatt tatttcataa atttccacat aaagatgaag gttttctcac
    20341 tgagatacgt tcacgaattg taaatactga aaatctttgt aaaattgcta aagaaacaaa
    20401 tctcatagaa atagttgaga aatttttgcc tacacaacac aaaactcccc caaataactg
    20461 gttggggaat actctagaat cattcattgg agctatattc cttgataaag gttatgattt
    20521 ttgttataag ttttttattt ctcttataaa agataaaatt attgatattg aaaaaataat
    20581 tagtgaagaa aataattata aaagccgatt attagaatat tgccaaaaaa ataaacttaa
    20641 tttagaattt ttctctgaaa aattgcaaaa taataatttt aagataaaaa ttttaataaa
    20701 taacaaacta ataagtgaat atatagctcc aaatataaaa cgtgcagaaa aggaagcttc
    20761 taaaatagca tacgataagc taataaacaa aaaagcaact atttatgtat tgtaaattga
    20821 tacataataa acaatattaa aattttgata ctaatatttt aattattgtg atttgtttac
    20881 actatgaaaa aaatatcact taactcttat ttatagaaat atatcacaga atggagtaat
    20941 tttaaattta gaatttttgt ttaaatcatt ttttgtattg ttaattaaca aaggtttatt
    21001 ataatttttt cataattaaa agcttttgac aaattaaaaa aaaatctgta aatttgtaat
    21061 aataaatctg aaataatgaa aaatgcaatt tcaatatcat taataatttt ttgcattatt
    21121 ttcagctcaa accaatgtaa aaataaatca cttatgtcaa aagagataat agtaaaaagc
    21181 aagtcattta ttgatggggg catgatacca agtaaatata cttgtgatgg tgctaatgta
    21241 tcgcctcaga tttcatggga aaatatacca gagggcacca agtctttagc tctgatatgt
    21301 gatgacccag atgctcctat ggggactttt gttcattggg tgctttacga cattccacct
    21361 atgattaaag aattaccaga aaacatacta cccgaaaaaa tattgcctag tggagctaaa
    21421 catggagtca atgattttcg taaatatggc tatggaggtc cttgtccgcc gagtggcact
    21481 catagatatt attttaagct ctatgcatta gatactatgt taaatttaga acctggcaaa
    21541 aaaaaagctg aacttttaaa agaaatagaa ggccacataa tagctcaagg acaaataatg
    21601 ggcaagtata gtagaaacta aataaactca aaaattatta atatgcatgt aaatcttgca
    21661 atttttgctt ctggtaatgg tagtaatgct gaaaatattt atcattattt tgatggtaat
    21721 caaaatattt ttgttagcct tttattaact aataataggc gagctggagt tattgataga
    21781 tttataaatt ttaatgttcc gatagtggta atagataata ttttagctag tgacggaagt
    21841 tttttaatta ggctttgtga aactttcaat ataaatataa tcattttagc tggctattta
    21901 agggctatac cagcagattt aataaaaaaa tatcctgata aaatattaaa catacaccct
    21961 gcccttttac caaaatatgg tggtaaaggc atgtatggtt ctaaggtaca tgaaaatgta
    22021 atagctaata atgaaaaaac atcgggtatt agtattcatt tggtaaacga aaactatgat
    22081 gagggacata taatttttca aaaaaccatt tctatcgact caacagatac tcctgataca
    22141 ttggctgaaa aaattcacaa attggagtat gaatattatc ctatagttat cgaaaaatac
    22201 ataaatgaat tatttactta aattttaaca aaaccaattt aatttattga aaatgtctaa
    22261 ttatcttact gatcatgaat ggcaaaaaat aaaacaaatt tttattaaac aatctataag
    22321 tgaagttggc attagcgaat ctgatgaagt gcctgaaatt gttaaaggaa attttttagc
    22381 atatttacaa tctcattata atggggatat gaagtattta gaaaggaatt tagaaaaacg
    22441 tttctcccct actacactgt atcctggcac tcaaagtatt atcacttttc tcttaccata
    22501 ttatcaaata tttcctgctg agtgggcaga aaagattagc atttatgtta ctaaaaaaga
    22561 ttatcatact cttgcaaaat caatatctat accagtagta aattttttaa aacaattaga
    22621 cgctaatttc agacctagaa tttttgtgga ttcagcacct gttattgata aatattgggc
    22681 ttggaaagca ggtttgggat ttataggtaa aaatacttta ttgataaatg aaaaatatgg
    22741 tagctttgta ttcatagttc atattttcac ttcactaaaa gcacctccag atgcattaaa
    22801 aaatccatta aaaaataaat gtggtaattg cactaattgc cttaaagctt gcccaactca
    22861 agcattgata tcacctaaag tattgaatgc aagtcaatgt atttcttatc ttacaataga
    22921 aaaaagtgat aataatagtt tgcaccaagt aaaaaaactt gtaccatata tttatggttg
    22981 tgatatttgc caaaaaaaat gtccttataa taataatcct cagataacac cgattaaaga
    23041 ttttttattt gatgaacaag aaaaacaaga attaaaaaat tggggaacag ggatctcaaa
    23101 atttccttta taccttttaa atgattccgt attaatgaag aatgtaaatt ttatcaaaaa
    23161 taaatataaa tcataataga tttatggtga tcagcaatat ttaaataata aattttttta
    23221 taaagattta tattttatgt atttaatcat taacttcatt ttttttatta attatttgat
    23281 tttcatatga taatatttca tcatttataa aataaatttc tatatttgct tctttaaaaa
    23341 ggtcttcact atctttgcct gcatgatacc tgtattcaca gacaacgcgt ttgatgccac
    23401 tatttatgat caacattgca catgagcgac atggggtcat tctgacgtat aaagtagatc
    23461 cttctaaaga aataccaaag cgtgctgctt gtgcaatagc attttgttcg gcatgtacgg
    23521 tcctcacaca atgttgggtg atagaaccat cttcgtgaat aatctttctg aataaatgac
    23581 caacttcatc acaatgtggc aacccactgg gagcacctac atagccagtt actaaaatac
    23641 gtttatcttt tactatcacg caaccagctc tacctctatc acatgtagcc cttgtagcta
    23701 cagtattcat gatttcgata aaatattcat cccagctagg tctttggtaa atgtatcctt
    23761 tttctttact ttcaattttt tcctttattt ctttcattat ggcatccacc tgatcatata
    23821 attgctgaaa ggtcccattg ttttctatgt aataatcagc taattctatg catttactta
    23881 tattttgctt attaggatct tcatttttca tttcttcatt ttcttgtgca atgaaggtag
    23941 cataatctat atcatctgtc tctgaccctc gctgacgaat acgttcgtat cttattttag
    24001 gatcagcatc gatagctatt aaaaaaaaat tatcattttt tttcaatgct tctacttcac
    24061 ctaccgtacg tatactttct attatcgcat ttttttttgt ttttatagct tctgagtata
    24121 atttttctac aatatatgat gcaccatttt tttctctaag ttcattagct aattgtctca
    24181 tagtatctct attaactgga attttccgta ttttaagttc tttaatcaaa aatttacgaa
    24241 cactaaaatg ctcaaaattt tcatttttaa tcagataatc taccactgta tccttacctg
    24301 cccctattgt acctgttagg cctattacta tcattttttt tacaaaaata agattaaaat
    24361 ttttatttga aattaaatta ttgaaaaagg taaatcctag attattgatt gctaattaaa
    24421 aaaaaagatg ttcaattaaa attaattatt tattatcatc ttcttttatt tctttatcat
    24481 aaactaaaag agcatattca ttatctataa tcagataccc catatcatag caaaaaaaat
    24541 aagttgtacc tttagcagtg ttcaaaatat gtgtatgata tttaaaatca aaaccttttt
    24601 ttcttaatgc ttcaattttt atttttgttt taccagtttt attgagttcg gatataatcc
    24661 ttctattttt tcttaatttg ttattaattt ttctataaat atttatagaa tctctattta
    24721 atctattatt ataagcatta cgacattgat catcacaaaa ttttttatct gaacgaccta
    24781 ctaatggttc tccgcattct agacaacatc tctctgtcat ggtactatga tttttttagt
    24841 acaaatttag aaaaattttt agtaaacgaa tattcccata tacaatataa aaaaacacta
    24901 ataaatataa gatcaaaaac agtaagtatc atatttacag atatattagt aactatctgc
    24961 atagcgaata ttagtggtat gattaaatat actttatgac taatattttt ttttaaataa
    25021 aaacatataa aaataccaac caaactacta actaaaataa aaataatata gaaagacaca
    25081 gtattataat taacataact ccaaatagct cctatgagct ctagaataaa ccaaagcaaa
    25141 aaaaaactca aattgatctt atatcttttc ttggcttctt gctcaccaga ataaatttta
    25201 tcattgtata taataaaagg tctattagaa tattttccat taaggctaag ctttattttt
    25261 ttattttcta agtctaaatc gacaccattt ttcattgatt cctttgatat atcacctatt
    25321 ttatcatcat tgaagtatat ccgatacaat ttttctttag gagaataaga aataataaga
    25381 gtgtcattat cctgtgttat tctataaata ttcattttta taatcggcta tttataaaaa
    25441 tataatttag ctgatgccat gtattttgcc atcaatgtcg atatctattt tttcggcaga
    25501 tggttgctca ggtagtcctg gcatacgcat aatggcacct gcaataggga ctacaaaacc
    25561 agcgccagta gatatttcta tttctcttat gtttaaaata aaatctttag gtctacctct
    25621 cagattagga ttatctgata atgaatattg agttttagca atacacacag gggaattttc
    25681 taaatttaac attttaatta gttcaagatc tttacgagct tttagagaat attccacatc
    25741 tttagcacca taaatatttt tacatatagt atttatttta tcttcaatag ataaatcata
    25801 agtataaatg ggtttgaaac aatttggaca atagctagta ctctctatta ctttttcagc
    25861 tagatctata gcaccttcac tacccttatt aaaaccttca ttaattgaca tcaaaacacc
    25921 atattctttg caatgttgcc ttactatatc tatttcagct tctgagtctg tatcaaattt
    25981 attaagagct ataataggtt taaagttaaa catcatcata ttttcaatat gtttatccaa
    26041 attttctaat ccttttttta ctaaatctac attaggaata aataattcat cttttgaagc
    26101 accaccgtga tattttaaag ccctaatagt aaccactata acgacagcat taggcatcaa
    26161 accaaatctc tgacatttta tatcaaagaa tttttctgct cccaaatcag aaccaaatcc
    26221 agcttcagta attacaacat cgctataagt cattgccatt tttgttgcta aaatactatt
    26281 agtaccttga gctatattag caaaagggcc acaatgtatg atagctggcg tgccttctgt
    26341 ggtttgcact aaattaggat ttatagcatc ttttaataaa gcggccattg ctccttgcgc
    26401 attcaaatct cttgcaaaaa ttggtttttt atcccaagtt aatccgacaa atatattgcc
    26461 taatctttct ttaaggtcac taatgctatc tgtcatacct agtatagcca tgacttcact
    26521 agcagcagtg atttcaaaag atgattctct tggtatgcca ttagagatgc cacctaatcc
    26581 gataacaata tctctcaaag ctctatcatt catatccatc actctattcc aatgaatagt
    26641 tctagaatct aaattcaaat ttctattttt actttgaaga ttattatcta ttaatgcaga
    26701 caaaagatta ttagctcttt ctattgcagg tatatcacct gtgaaaagta aatttatatc
    26761 atctgtaggt aatacttggg acaatcctcc tccagtggct ccacctttta ttccaaatac
    26821 tggccctaat gaaggttccc tcagagctac aacagatttt ttcccaattt tatttaaagc
    26881 catagataag cttatagaag ttgtagtctt gccttcacct gcgggagtag gcgtgatcgc
    26941 agttactaat actaattttg attgcttgac tttctcttca tttatgagat taattggaag
    27001 cttcgcttta aattttccat aaagttctaa ttgatcagct tgaattccta aattctcggc
    27061 tatttctgtt atctgtttca taatatatta atattttttt atgtttattt ctataaattt
    27121 tctaaatttg ccaataattt tttatttttt catctatata atttaaaatt attttagtag
    27181 ctcctatatt ggatttcaca tattctctac tcacttcaca agcattatta taaattattt
    27241 tatcattata caatatattt aaaatatcaa aatattcttc tttatttgaa aaactaaaag
    27301 caccacgtaa atttattaaa tctatagcct cttgaaattt tttataattt ggaccaaaaa
    27361 ttataggttt accataaata gcagcttcta aaatattatg tataccacta ccaaagccat
    27421 tacctatatg tacaatatta gcatatttat aaatatgtga aagtaaccct ataatatcta
    27481 tgattaggat atttttattt tttacgtcat taaaatttga attttctgat tttaactgcg
    27541 aataagtaat acaatcatca ttaaaagaac tttttagttt ccttattcta tcttcatgta
    27601 cttcgtgtgg agctatgata taacaccatt caggatattt atttatagca tgtagtatat
    27661 atttttcatc ttctggccag ctactaccag cgatatatac aaatctatta tcaataaaag
    27721 tttctatatt tggtatatac aatggattat tagcgatttc atatactcta tcaaatcttg
    27781 tgtcgccagc tactgttaca tttagtatca tatatttttc taataataat ttagatacat
    27841 tattttgcac aaatatatga tcaaaatatt tgagataacg tctcatgtat gcaccatacc
    27901 atttaaaaaa atattgatta ggtctaaaat tagcagatat taatattgta ggaatatcat
    27961 tccttttcaa ataatataaa taattaaacc aaaaatcata ttttacaaaa attgccaatt
    28021 caggtaaaaa agtagagata aatcttttaa tattacgtgg cacatctaat ggaagatata
    28081 aaactaaatc tacattaggg taattttttc taatttcata accagaaggg gagaaaaaag
    28141 ttagtaatat tttatgttca gaatatttga tatgtaagcc atcaatgact gttctagctt
    28201 gctcaaattc tcctaatgat gatacgtgca cccaaatcca tctatcggta ggtgagagtt
    28261 ttttataaat atctttttga tatttcctgc cctttaccca caattttgct tttttatgac
    28321 ccaataaagc acctaaattt attataaaac cataaatata aatgccaata gtatacaaaa
    28381 atctaataat catgatttaa ttaaagtgag tgcaaagata aataaaatat ttaaaattta
    28441 taattataaa aatcgacata tggctaaatt ttttttaaaa tatctatcat aaaaatatca
    28501 gaaacataat ctctttttcc ctcttaaaaa ataatatcat taattcttgt ttggaattaa
    28561 attttactta tttgctgata taaataatca ccaattgatc aataaataaa atattaaaat
    28621 tataataatt ttaatatttt tgtatcaaaa ttgttttatt ctttttttat tattaaacat
    28681 aaaaaatatt tttatgaaaa aaattagatt attatttgtt tttacattta tttcattttt
    28741 atgtttttct caagatagca taaaagtaaa atggagattg tctagtattg attttagttt
    28801 tggatatttt aatattacta attttgaaac tccaacctct tggccaccat atattcaata
    28861 ttttaaaaat attgatgcta ttaatgatta ttattatgac gcaaataatt actggactag
    28921 ctatataaat aaaaaaacag atggttcgaa tattaaattt ttattaggtt ttcaattgcc
    28981 taaaatgcaa ataaaaaata atagggaatt tttatttgga ataaattttc accaaaatgt
    29041 aaactttaga cattcactga ttgtactaga aagacatcca atagatactt ttattaatgt
    29101 acattcagga caagatttat tttttgactc tacacatgtg atttctgatg attataatta
    29161 ttcatttaat ttattagatt ttgattttgg ttataattat atgtttttta ctggcaaaag
    29221 atatcaaata catctagcac catacatagg attaggactt actataaata gacatgcgta
    29281 tatgaacagg tatttatatg ataaatacat gtttttttta ccagaagatt cattatttgc
    29341 acaaagtgaa ataattaaaa agtatgaatc attagaaaca atatatgatg ataatataaa
    29401 aaatcttttt tatttaaaat ttgggttgcc cattagaatt aatttaggtt taggaaatga
    29461 agataaaaat ttagatgaaa tatctttgtt ttttgaaata aatccgcatt atttatatat
    29521 accatcacat gatcctgtga tcagtagttt tactttatct tgttcctttg gtattaagat
    29581 taaattttta aaataaaaaa agcaccattg ttacaaattt taatttaaaa atttctccat
    29641 aataataaat ttaacaaaac tagataagat tagagacatt gacaagctgt tttaattata
    29701 aatgaaataa attttcttga tatcattatt ttaataattt ttttctaatg gtaatattgc
    29761 aatgatatta aaggttcttg aaattaatta ttgttttact ttacgtattt tatatgacca
    29821 ttaaataatt tttatttaat tttacaaaaa aataattatg gataatttta atctattaga
    29881 ttatcaagct ctacccaaag agcagaaacg tagattccta gata
//
LOCUS       DAOPRN010000022        29813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1224_length_29813_cov_116.998185, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000022 DAOPRN010000000
VERSION     DAOPRN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29813
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1224_length_29813_cov_116.998185"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..4825)
                     /locus_tag="PKI65_03975"
     CDS             complement(386..4825)
                     /locus_tag="PKI65_03975"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOB77634.1"
                     /translation="MSLHKGKKIFKNTILVLAWIICAVPAILIITLKTPTTQKFLIQI
                     FENWWQNNLNAKLEIGAIKIATFTNIELIDVKLYDQLDSTLIAVSSLKAKIKNIAPFS
                     KPFKMEFKDAIIRDPYVNLRIYKGDTTLNMNFIFEFFANEKEKSTPHIFINNSKISNG
                     IVKYVDENTHPSDSTFNYDAIEFFDLNLWISDFQLMDDNDISVKIHNLSAKEKYGFQI
                     KHLDGDFTMTSKHFALNNAHILTPQTYLNTDLLFRYKSFESFQYFVDSVEMNMTFRPS
                     NLSFIDISFFAPELKGIIIPIRLYGSVNGYVNDLHSDKLKLVLGKGSIIDLQFAMKNV
                     TYPNILEYSLALNGRNISNEDISAINIKGKSVLPTEIHLPEISILTLNAKGQGLAEKI
                     DLQFSLENPNEDLFISINGNNNIYIGQTRFNNFNFSQINIPNNPLQYLDGQLAFNFTN
                     LDVNRFNLLKLSGNIDIDLNKILISDIELNEIKGLINKKDTNYYAKINSYDPNTRLDL
                     EAEAQYSKLLSTIKLKLDAYNINMNKLKASRNDSLTLVSAFIYADFKGQNLDDITGNA
                     KITNLSYSEGKKRGTIDSLILTIVPISDEEKKIKIISDLLDLELNGKIYLSSIDKSFK
                     EYIYAYLPRLMKDTLDKSIEFYQDFNFSMHIKPPIQNFIDIFIADMTVSQVYLEGAFK
                     SSTNELDFDILIPYAAYNNVVKADSIHINLETFNNVAYLNLDLDRVINKDISASNIEL
                     LSTINRNKIDFQIKIPDADIYYPMNADIQGCATINEVNEINLSFYPSFISIADSTWYI
                     SEPSQINFINDHIDISYFELATNTQSIKINGIYGKRPDESISVILKNLQLAQIKNFVT
                     DFQYDIDGNIDGEVELSNFTQKFPNFYADLKFTNLIFNGQKIGNMEFTSVWDTIRSGI
                     KADLQLKEIGSLGVRIPLEAHGYIYPTGKQNMDLKINFNNLNLAILQPLMSSFASNVS
                     GIANGYLNIEGSFSAPQLYGELDLIRTRLKVDYTNVAYSLTTNIKINPNEIIIESLTI
                     NDPNANTANGYFIMRHNNFSDITLDTRLTFNKFLAVNASRDQNEYFYGKGLISGALRV
                     QGPINSIKIEGNITTEPGTTIFIPIKSSSVVQQSDFIHFINPKDTITDTLKVETPRQI
                     KSDKTTIAMNLRVSPSPETEINIEFDPRIGDKIMVKANGEIQVTMDTDGNLNLLGDIT
                     IVDGYYNLTMQNTINEKFNIDPGSVIKWRGNVMNGEMNITARNNVRTSLYNLVGHLDS
                     SEVYKQKYLVSVLLHITGNFLSPEIKFAIELPEADEDTKNLVKNVLRTEQDVIQQAFS
                     LLLFSSFMPAVGTRGMDIGSGIGTTSFEMLSNQFSNWLSQITGNVDVGFLYSPSETQI
                     SLSTQLLEGKLQISSSVGVNTYDAPYSNNSSNFVGDFDLSYKFNDNLKLRLFNKTNPY
                     DQLIYYGPYTQGIGISYSYSFDRIKKLWNKSNDDDKKSKIRVK"
     gene            4830..5873
                     /gene="tsaD"
                     /locus_tag="PKI65_03980"
     CDS             4830..5873
                     /gene="tsaD"
                     /locus_tag="PKI65_03980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOB77635.1"
                     /translation="MSLIVMGIETSCDETSVAILHDDTLISNITATQDVHASYGGVVP
                     ELASRAHTQNIIPVIHTALQYANLHKKDVNVVAFTLGPGLVGSLMVGISFAKSFAQAL
                     NLPIVAIDHLYAHVFASFIQFPNEKHEVPSFPFLCLTASGGHTQLLRVKDFTDIEIIG
                     QTIDDAAGEAFDKAAKILGLPYPGGPLIDELAKKGNPFYFSFPKPKVADFQFSFSGLK
                     TSLLYLVRDNLKDDPGFINKYKEHLAASYQRTIIEILTEKTFKAAETFGFKNIVLAGG
                     VSANSELRKTFIDKGKELGYKIYIPPLSLTTDNGAMIAFLGKILYERGITSSLDVKQY
                     ATGEKYKKYHSKG"
     gene            6012..7010
                     /locus_tag="PKI65_03985"
     CDS             6012..7010
                     /locus_tag="PKI65_03985"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77636.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFKKFESKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEIVTSHRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNLTYVD"
     gene            complement(7407..9065)
                     /locus_tag="PKI65_03990"
     CDS             complement(7407..9065)
                     /locus_tag="PKI65_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOB77637.1"
                     /translation="MVRNRSIFLIILILLSGIKINSQIYYRVDFSNKFNTNYSLDQPE
                     LFLSNKAIERREKFNIPIDSTDLPVNSWYLDSLTSLDCEVLYTSRWFNSAVIKIQNDN
                     IINRILQLPFVVNAQLVKPKINKETDETKITTPSIAYSDITKKSTKIPQTIYGFSTNQ
                     NQMIGIHILHQMGYKGDGIVIAVLDAGFSGCDHIPFFDSLRLKSRLLGTKDFVFPGNN
                     VYNKSNHGTSVLALMASNIPNILVGTAPEASYWLIRTEDPSSEYLIEEENWIAGAEFA
                     DSVGVDIINSSLGYTTFNLNSQNHSYTDLDGKTTRVSKGAAMAASKGMIICNSAGNSG
                     NSSWYYIGAPADADSIITVGAVDSLGIVVEFSSKGPTYDGRIKPTVCAQGANVFTASQ
                     DSSIIMGNGTSFSSPIIAGATACLLQKHPNSDPISIITSIIKSANRYLTPNNFYGYGI
                     PNFAVADILANDTSISSPPWLESTPFIYPNPFTSYINIIYQSKEKSFLDIALYSINGT
                     QVFKNIYQLFPGNNYIIIEQLDSLSSGIYIMAIGANKTIKVVKQ"
     gene            complement(9052..9723)
                     /locus_tag="PKI65_03995"
     CDS             complement(9052..9723)
                     /locus_tag="PKI65_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77638.1"
                     /translation="MKHHIILIILIFALFSCQSDIETIDLGYDYYITKPGYYLIYTVD
                     SICFDDFYNTSDTTNFLLKELMESTFVDNEGRMAFRIERWHKYSDTLPWKLKNVWYCV
                     KTNQRVERVEENTRFIKLIFPIKNFYRWDGNALNNLGYQEYKYQNAHHPLQILNFYFD
                     STVKVLQDSFVNLKYVRYKYEIYAKNVGLVKKVFVDLENKDNLSGEIKKGVKYEWKLI
                     EYGTQ"
     gene            complement(9720..10532)
                     /locus_tag="PKI65_04000"
     CDS             complement(9720..10532)
                     /locus_tag="PKI65_04000"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOB77639.1"
                     /translation="MKCPFFIFLLLITINNQLICQNDTINLRDNNGLPHGYWEKYKDD
                     RLFYQGHFDHGIPIGEFTYYYPEKILKSKLIYSDNGKKAYAIHYYKNGKKYAEGLYYN
                     KKKEGKWIYYDGYDHIIAIENYKNGELNGFCYDFFPNGDTLQIANYINGKRNGEYKLF
                     YPTKNPRIITTYKNDTIQGLTKYFYSDGQIYEMGKYLDGLRDGLWIMYDEDGVVILTE
                     TYYKGTLIERKVYQPDKDPIKIENEIYENMELYKKGLIKEPSESDFFNDTFR"
     gene            complement(10535..11125)
                     /locus_tag="PKI65_04005"
     CDS             complement(10535..11125)
                     /locus_tag="PKI65_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOB77640.1"
                     /translation="MSAYNQKVLSEWNIHKNWTLFLDRDGVINKRKIGDYIKIIDEFE
                     FIPNTKEALAILDKYFNKIIIVTNQQGIGKGLMSEKDLQEVHNYMHEEILRAGGRIDA
                     IYFCPDLASSNSQYRKPNPGMALKAKEDFPVIDFSHSMMVGDTKIDMEFAQNLGAKKI
                     FIGDLEEDQLTIMDIDLVFNSLYDFALEIHKYYQNL"
     gene            complement(11128..11697)
                     /locus_tag="PKI65_04010"
     CDS             complement(11128..11697)
                     /locus_tag="PKI65_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOB77641.1"
                     /translation="MEDIKNYINNSIKVKKKIITNDELINNIENLIEISIINISKGGK
                     IFFCGNGGSAADAQHIVAELTGKFLNDRQPIPAIALGTNFSYTTAVANDYNYDNVFVR
                     ELVALAKPYDILVGISTSGNSPNIIKVINKAKEIGLVTSGWTGETGGRLKDLVDIWLG
                     IPSDFTPHIQEAHITIGHAFCYFLENKLK"
     gene            11841..15383
                     /locus_tag="PKI65_04015"
     CDS             11841..15383
                     /locus_tag="PKI65_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOB77642.1"
                     /translation="MKKVLVILFALLSINFVFAQTKEVTFPINDKQIWQIVDNEIMSF
                     RVKISLPAIQLQNVNTPHGIFTQIIGDALAISYDTGKAQLPTLNHLIEMPSENLNITI
                     ISKKEQIINLNELGFTSPIIPAQPSISKSTDPKTVPFYFDENYYNTNVFHRKQLAEIE
                     YYGKQRGTGIGLLRINPFAYNPVTNLLEIIYEVELEVTFNSNNIKDYLNDKLRLFSPF
                     FNFSNLLNYQNNVIKDVITDYPITYLIISPRSFENTLQPFIRWKAEKGYNVEVAYTDV
                     IGTSTTNIKSYLQSLYSNTTPPPTFLLLVGDVDLIPSFNGTTQNSHVTDLYYACYDGS
                     SDYLPDLYYARFSAQNATHLQNLINKTITYEKYQMPIKSYLDTVLMVAGVDNNYSSTY
                     ANGQINYATQNYFNSNNGIYSHTYLYPASSGSGVSADMISKISRGTGYANYTAHCNSA
                     GWGDPSFTTSNVASLQNTHKYGLMVGNCCQSSKFEINECFGEAITRATNKGAVAYIGA
                     SDYSYWDEDYYWGVGYRSNIVANPTYNSTNLGAYDRVFHTHGEPYSEWFITNAQMIHG
                     GNLAVQSSNSSLKKYYWEVYHLFGDPSTMNYFSVPDPLTISYNEPLIIGDNSLMVETE
                     PYTLVALSSNGILLDAQYTYNSNSVNLTFQDLATDDSLLIVATKQNKVPYIEKIEVNE
                     NLLALDAKISELLAPVSSYNCYDIDITPRVTIQNLSTQNLISLNVCYSYADQPLQCVN
                     WNGNLASLEKDTLDLPSLHLLEGDNQFIVFCENPNNASDMKPSNDTISINVKSEYKNI
                     ISNFEANVTSGCNLPFIVNFSNLSQNALSYLWDFGDGYTSTDYEPSHVYTSYGLYTVN
                     LIADAGVCGSYTETKVNYIQVGSLPPTVHDTTICIGEDVILEAYADGLVNWYINMYDV
                     SPIFTGNYFSISNLTHDTTLWVDNYVESPIQHVGSTQSNTNGGFFNSWTTHYLIFDCY
                     QPVTLISVEVNAKTDGNRTISLRDENDNTIQSTTINIPAGISRVTLNFDLPVQNNLQL
                     SCTTFPNLYRNSSGAQYPYEISNMISIKGNSAGDPDYYYYFYDWEIKGVDCISERIPI
                     TAHVDNCDEIKFFISNEFMIYPNPASDFITIELNEAIPTKLNIFSLDGRLLRDYYFSN
                     NKFVLDVSNLTPGTYLLRLSNDNGTSQKIFGVFH"
     gene            15508..15580
                     /locus_tag="PKI65_04020"
     tRNA            15508..15580
                     /locus_tag="PKI65_04020"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15541..15543,aa:Phe,seq:gaa)
     gene            15799..15870
                     /locus_tag="PKI65_04025"
     tRNA            15799..15870
                     /locus_tag="PKI65_04025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15832..15834,aa:Arg,seq:cct)
     gene            15887..16594
                     /locus_tag="PKI65_04030"
     CDS             15887..16594
                     /locus_tag="PKI65_04030"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOB77643.1"
                     /translation="MGYTICIDFGNTSTKVSLNDAIFSLSTDEYIEINIKNILKKFDI
                     THGLYSSVITLSDGLQDLLEKNNVLSIKSKYSNLPFSIDYQTIDTLGEDRIAASVGAY
                     SLKKNKTFLTIQVGTAITYDYIIKKTYVGGAISLGFDIRYKALHNFTKKLPLLTSEDN
                     KNIKTPMIGNNTNDSIHSGVYWGVIYELQARIDEFIKKYKGPVYLTTSHKENIYLKNC
                     NFAEQNLVLLGLKYLLK"
     gene            16591..17868
                     /locus_tag="PKI65_04035"
     CDS             16591..17868
                     /locus_tag="PKI65_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77644.1"
                     /translation="MKFFKNIYFVIIFFMLITTFIMGQDNFYNPYSRYGIGDLQNTNH
                     SLIYGLGINGGTFYDKSIINYLNPATYSVFDSSSVVINIGANAIGNILENSKQSSYKW
                     GGGLSMVGGGFRINKFLSTAIGLVPFSNVGYKIVDSMPQFTSSSYNNVYSSHGGINQA
                     FLGFGINITKNFSLGINFSYLFGSIYQSKCTDFDSTYFFDNRYINETFVNDFYFNAGL
                     HYLIPMQKSNLSFSFTYAPELSIDASRTIRSELVTFGIYNDIGIDTTAESKIKGKYEF
                     PAFYCAGISWDNKENIKIFIDGYFSKWKNFKNFGETDSLQNSFAIQSGISFIPKPNSL
                     KNIFLRSNYIISFKYAKTYLNLRNTSLDAYTFSTSMLLPFRPIFKSVSSIGINFAYTY
                     LGTKKNNLIAVNNFNIGISIHLKEGFYERKKYE"
     gene            17914..19335
                     /locus_tag="PKI65_04040"
     CDS             17914..19335
                     /locus_tag="PKI65_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77645.1"
                     /translation="MKRLVLILVIFIGIGAMNAQQTKQITFPEPCSINGPKWGPDSVK
                     AVQSYSIYRENMKQWNNNKERTELIEYSLDSWRYLFNNAPLASAYIYIDGQDIIEYLI
                     KKNESNPELKQKYIDTLMMVFDKRIEAFGCESHSSESFVLGRKALAMLQYRPENIDEI
                     LATFRRSIELGKEDAEPAIVFTYYDFLVNQVVNGKMDTIVIFEEYDRLSDLMNKRLVK
                     LYDEYKNNPADSNRYIRSINNVKAAIINMDNFMAPFANCDIIINIYSNKFEQNKQNID
                     FLRRLTNIMEKKGCDDNKLYFKAAEQLYTLDPSPASAISLAKSFIKTKRYSDAIKYAN
                     QAAENFQDPDDKFNAYMVLAEAYRLNGQYSAAKNAAIKASNIKPNSGQPYIFIGDLYM
                     ITSASCGDNPVTKRAGYWAAYDQYIKAKRDPEFANIASRKASEAYSYFPKTEDLFFYG
                     YYKGNSYTINCWYTETTTIRASD"
     gene            19343..19903
                     /gene="lptC"
                     /locus_tag="PKI65_04045"
     CDS             19343..19903
                     /gene="lptC"
                     /locus_tag="PKI65_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOB77646.1"
                     /translation="MRFLSCLFKFFIVLFLFLAILGCQPSTLEEIKKVTEQDTFPIAT
                     IKNASVYYSDSGRVTMHILAPIIKNYIHPNEVTILEGDLKGNFYDSIGNVQSIIYAKY
                     AKLDDKNAKLELRKDVKVFNIIDNTEFYSEELVWNRRSHIITSTKFIRIVTPDKIIWG
                     EGFSSDEELKNYEILHPKGELYIDNI"
     gene            20006..21092
                     /gene="prfB"
                     /locus_tag="PKI65_04050"
     CDS             join(20006..20062,20064..21092)
                     /gene="prfB"
                     /locus_tag="PKI65_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118304733.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOB77647.1"
                     /translation="MSEILNDLKGRLASLRRFLDIDNIKKEIESQETKTQEPNFWDNP
                     KEAEKILKEISNKKNFIEKYNEVARKIEDFSVYDEFFNAGEISDTEYQKQYELVKKAL
                     DRLEFYKMMHDEADKNSAIITINSGAGGTESQDWAQILMRMYIRWAERHNFKVIELDL
                     QEGDVAGIKSCTLEIDGDYAYGYLKSESGVHRLVRISPFDSNQRRHTSFASVFAYPLI
                     DETIDIEINPADLEWDTFRSSGPGGQNVNKVETAVRVRHIPTGIVVECQVTRSQQQNR
                     EKAMQLLKSRLYEMELRKKQEELDKLEKNKKKIEWGSQIRSYILHPYKMVKDHRTGIE
                     TSNVNAVLDGEIDDFIHTYLLSLNINF"
     gene            21129..23117
                     /locus_tag="PKI65_04055"
     CDS             21129..23117
                     /locus_tag="PKI65_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOB77648.1"
                     /translation="MEKEEFRLPENAYRELNEGEEYKPILDPTKKYKEVTTWSVAMGI
                     LMAIIFSAAAAYSGLKIGQVFEAAIPISIIAVGATMAARKSDPLSQNVIIQSIGASSG
                     VVVAGAVFTIPALYILQHKYPSIEINFWPIFFSSLLGGFLGILFLIPFRKYFVKDMHG
                     KLPFPEAKATTEILVTGANVSKQSSILLKGGIIAGIYDFMINSLKLFQEEFTSRMVGW
                     GAAIADKTKVVFKMNASALVFSFGYLVGLKYALIIVMGSLLSWWVFIPIIGYIGGMQD
                     LSAELIFLDYVRPIGIGAIAMAGIIGIINSLPILSKSFGLLFNKKKVSETEVLRTDKD
                     IPSRFWLSALILVIFGILLFFILSVNLNWWQAVIVLVALLIISFLFTTVAANAIAIVG
                     TNPVSGMTMMTLILMSLVLVKVGLKGTTGMVVAMLVGGIVCTALSTAGGFVTDLKIGY
                     WIGSSPYKQETFKFLGILVSAATVGIVIFVLNQAYGFVKTPEHPNPLVAPQANAMASI
                     IEPFMQENAEIPWILYLVGAITAIIINFLGIPPLAFALGMFIPQGLNTPLLVGGLINH
                     FAANKTKNKELAKAREQRGTLLASGFIAGAALFGVIGALLLFFGLDLTVPIFKELVNG
                     KWVWIDGNVWPEVLSLVAFVLIVTYFIWEIRRAKIEDN"
     gene            23274..24896
                     /locus_tag="PKI65_04060"
     CDS             23274..24896
                     /locus_tag="PKI65_04060"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOB77649.1"
                     /translation="MTKDTKYIFVTGGVVSSLGKGIISASIGKLLQSRGFSITIQKLD
                     PYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNIYTSQANNVTTGKIYLNVI
                     EKERRGDYLGETVQVIPHITDEIKYRIRLLEFSGDYDFIITEIGGTVGDIESLPFIEA
                     VRQLRLELGKRSLVMHMTLLPYLANIGELKTKPTQHSVKNLQELGVQPDILICRTSVP
                     INDSIRQKLALFCNVEQRAVIENIDIDTIYKVPIKMQEEKLDEVILDKFGISSTSQTN
                     LSKWIELINHIDNPKNEVDVALIGKYIELKDAYKSIIESFIHAGAYLETKVNLDFISS
                     ENITEGNLDNLLNKYNAILVAPGFGERGLEGKILAAKYARENKIPFLGICLGMQMAVV
                     EFARNVLHLKDANSREINPRTLNPVIDLMENQKKISVKGGTMRLGAYPCKIVKNSKLY
                     ECYKQDLVYERHRHRYEFNNKYLEIFESHGMKAVGINPTEQLVEAMELEDHPFFIGVQ
                     FHPEYKSRFESPHPLFVSLVRAALEKKNNIIQ"
     gene            24986..25069
                     /locus_tag="PKI65_04065"
     tRNA            24986..25069
                     /locus_tag="PKI65_04065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25020..25022,aa:Leu,seq:caa)
     gene            25141..26028
                     /gene="fabD"
                     /locus_tag="PKI65_04070"
     CDS             25141..26028
                     /gene="fabD"
                     /locus_tag="PKI65_04070"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOB77650.1"
                     /translation="MKKAYVFPGQGAQYPGMGKDLFDHFPIAKEMMDRANEILGFNII
                     KLMFEGSEQDLMQTKVTQPAIFIHSVVLANVLAERFDPHMVAGHSLGEFSALVAAESM
                     SYEDGLKLVYARAIAMQKACEQQPSTMAAVIGLDDKKVEEVLSTIKDEIVVPANYNTV
                     GQIVISGTLKGIEIAMEKLKEAGAKRVLPLKVGGAFHSPLMEQARAELATAIDKTPIK
                     IPRCPIYQNVTAMPTTDPNEIKKNLIDQLTSPVKWTQTIKNMINDGATEFIEVGPGNV
                     LQGLIKKISSDVNAYSATI"
     gene            26042..26575
                     /locus_tag="PKI65_04075"
     CDS             26042..26575
                     /locus_tag="PKI65_04075"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOB77651.1"
                     /translation="MDLSKIIYIPGKPGLYKIIGNNKTAFIVESLIDSKRLPVFMNSR
                     MSPLSDIVVVTKEDQVHVEVVFKNTLKEFDGKIINIDQNDEDSLISFMDKVLPDWDKD
                     AVTPKDIKKLIQWYNLLIDKSIISLEDLKEDSVESQESEVKDDSIADETQKEKSEISE
                     NEDQKQSDKTDEIEINE"
     gene            26568..27347
                     /locus_tag="PKI65_04080"
     CDS             26568..27347
                     /locus_tag="PKI65_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOB77652.1"
                     /translation="MNNKIIRTKLKVVNNEQINSSHFRLYLTMPDDCPPITPGQFAEI
                     YVPPTLNSFLRIPLSIHFISDKKNEIQFLIQLKGEGTKYLKTLKKNDFVDIIMPLGKG
                     FSIPRQRKLALIAGGCGVAPLLFLSDKLYEHGNELEIYLGFRNHNQVMLINEFKKYGK
                     LVIATDDGSMGEKGVITQVFERHLDDFDFIYACGPTSMLQSLMYLSEKYNIKCEISLE
                     TLMACGLGACLCCAVPTKKGNMRACVDGPVFDTKDLIELNE"
     gene            27394..28302
                     /locus_tag="PKI65_04085"
     CDS             27394..28302
                     /locus_tag="PKI65_04085"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOB77653.1"
                     /translation="MSTKIDIGKLTLKNPVLTASGTFGYGQEFESFIDPSLLGGIIMK
                     GTTWEPREGNPAPRMAETSAGMLNAVGLQNKGVHHFIDKILPAITKYDTKIIVNVAGN
                     DVADYVKTASLLNDIERVDAIELNVSCPNVKKGGIQFGTNENLLKELLSAVRHVYYKT
                     LIVKLSPNVTKIEDFAKISESEGADAITVANTFLGMAIDINTYKPKLSNITGGLSGPA
                     IKPIIMRMVYQCYQVVKIPIIASGGIMHWQDAIEYFLAGATAIQVGTANFINPSASIE
                     ILQDINDYLDNNNIESIKNIIGKVKI"
     gene            28603..29226
                     /locus_tag="PKI65_04090"
     CDS             28603..29226
                     /locus_tag="PKI65_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOB77654.1"
                     /translation="MLPKEIKVIIFDLGSVLLKVDYQRTIDSFKKLGMTYPKHYFNVE
                     YNDPILDKFEKGLLSENEFIKKIEMKISKVCSKKEIIDAWNAMIVEFPEENWLLLNEL
                     KTKYKLFLMSNTNIIHYNYCQNLVQKKFGVSSLDCIFDKAYYSFVVNMCKPDINFFKL
                     ILDEQKLITKEIIFFDDNKENIKTAEKLGIKSIRVQPNSSLTKYFDL"
BASE COUNT        10521 a         4122 c         4790 g        10380 t
ORIGIN      
        1 attatgtaga gatgtttctc aaacaggtca ttggggaaat ggcgatgttg aagttatttt
       61 agaatcagaa gaagagattg gttatgtgat tggtcttgtg cgacaggcaa ttgaaaaaca
      121 atttggaaat ggagaaagcc tatgaatata cttaacaaca acacaccccc acaagtctac
      181 aaaatgacca aacttgaaat actgccctag aaatgtggaa agatgtggct gggagagtag
      241 ttgaaatcat gagtggagga actccttcaa gaaaaaaaag aatattggat aaatggagat
      301 ataccatcga ataaatttat aaaaattata aaagcaggta aacttctggt ctattcacct
      361 tgctaatatt ttacaagtca cacaattatt ttactcttat tttactcttt ttatcatcat
      421 catttgattt attccataat ttttttattc tatcaaagct atagctataa gagataccaa
      481 ttccttgtgt atatgggcca taataaatca gttggtcata aggattagtt ttattaaaaa
      541 gtctaagttt taaattatca ttaaatttat aagaaaggtc aaaatcgcca acgaaattag
      601 atgaattatt agaatagggg gcatcataag tattcacccc tacactacta gatatctgca
      661 gtttgccttc taaaagctga gtagacagac tgatttgagt ttctgatgga ctatataaaa
      721 atcctacgtc tacattacca gtaatttgtg atagccagtt actaaattga tttgaaagca
      781 tttcaaaaga tgtggtaccg ataccactac ctatgtccat gcctctagta ccaactgcag
      841 gcatgaagct actaaaaagt aataatgaaa aggcttgttg tattacatct tgttctgtac
      901 gcaatacatt ttttacgaga tttttagtat cttcatcagc ttcaggtagt tctattgcaa
      961 atttgatctc aggcgatagg aaattacctg taatatggag taaaacacta actaaatatt
     1021 tttgtttata aacttcggat gagtctaaat gaccgacaag attataaaga gaagtacgca
     1081 cattattgcg agctgttata ttcatttctc cattcattac atttccacgc cattttatta
     1141 cgcttccagg atcaatattg aatttttcat ttatagtatt ttgcattgtt agattataat
     1201 atccatctac tatggtaata tcacccaata gatttaaatt gccatctgta tccattgtta
     1261 cttgtatctc gccatttgct tttaccataa ttttgtcacc gatcctaggg tcgaattcga
     1321 tatttatttc agtttcagga ctaggagata cgcgtagatt catagctatt gttgttttat
     1381 cagattttat ttgtctagga gtttctactt ttaatgtatc cgttatagta tctttaggat
     1441 tgatgaaatg aataaaatca ctttgttgca ccacgctaga tgatttaata ggtatgaaaa
     1501 ttgtagtacc gggttctgta gttatattgc cctcaatttt aatagaatta attggtccct
     1561 gcacacgcaa agcaccactt attaagcctt taccatagaa atactcattc tgatctcgtg
     1621 aggcattaac agctaagaat ttattaaatg ttaatcgtgt atctagtgta atatcagaga
     1681 agttattatg tcgcataatg aaataaccat tggctgtatt ggcatttgga tcatttatgg
     1741 ttaatgattc tattatgatt tcattaggat taatcttaat attagtcgtc aggctatatg
     1801 ctacattcgt ataatcaact tttaatcttg tccttatcaa atcaagttcc ccatatagct
     1861 gtggcgccga gaaacttccc tcaatattta ggtatccatt agctatacct gatacattag
     1921 aggcaaagct agacattaat ggttgcaata ttgcaagatt taaattatta aaattaattt
     1981 ttaaatccat attttgtttt ccagtaggat aaatatatcc atgagcttct aggggtatcc
     2041 taacaccgag ggaacctatt tcttttaatt gcaaatcagc tttaatgcca gatctgatag
     2101 tatcccacac acttgtaaac tccatattac ctattttttg tccattaaaa atcaggtttg
     2161 taaatttaag atctgcataa aaattaggaa atttttgagt aaaattactt aattctactt
     2221 ctccatctat atttccatct atatcatatt gaaaatctgt tacaaaattt tttatttgag
     2281 ctagttgtaa attttttaat atcacagata ttgactcatc aggtcttttg ccatatatac
     2341 catttatctt gatactttgt gtattagtag ctaattcgaa gtatgatatg tctatatgat
     2401 catttataaa attaatttgt gaaggctcag aaatatacca tgtactatca gctatagata
     2461 tgaaacttgg gtaaaaagac aaatttatct cattaacttc atttattgta gcacaacctt
     2521 gaatatcagc attcatagga tagtaaatat ctgcatctgg tatttttatt tgaaaatcta
     2581 ttttatttct atttatggtc gacaaaagtt ctatattgct agcacttata tctttattaa
     2641 tgactctatc aagatctaaa ttgagatatg ctacattatt gaaagtttct aaatttatat
     2701 gtatgctatc agcttttacg acattgttat atgcagcata aggaattaaa atatcaaaat
     2761 caagttcatt tgtactactt ttaaaagccc cctctagata tacttgtgaa acggtcatat
     2821 ctgctatgaa aatatcaata aagttttgta taggtggttt tatatgcatt gaaaaattaa
     2881 aatcttggta aaattctatt gatttatcca aggtatcctt cattaatctt ggtaaataag
     2941 catatatgta ttctttgaat gatttatcta tactagataa ataaatttta ccatttaatt
     3001 ctaaatctaa aaggtcagat attattttta tctttttctc ttcatcagat ataggaacaa
     3061 tagttaaaat cagagaatct atcgtgcctc tttttttacc ttcactatat gataaattag
     3121 taattttagc attgcctgtt atatcatcca aattttgacc tttgaaatca gcataaataa
     3181 aagcagaaac caatgtgagg ctatcatttc gagaagcctt tagtttgttc atatttatat
     3241 tatacgcatc taattttagc ttaatggtag acaataattt gctatattgt gcttctgctt
     3301 cgagatccag tctggtatta ggatcatagg agtttatttt agcataataa ttagtatctt
     3361 ttttatttat cagtcctttt atttcattta gttcaatatc agaaattaaa attttgttaa
     3421 gatctatatc aatattgcca ctgagcttta aaagattgaa cctattaaca tctaaattag
     3481 taaaattaaa tgccaattgt ccatcaaggt actgtaaagg attattagga atattaattt
     3541 gagaaaaatt aaaattatta aatctcgttt gaccaatata gatattatta ttgccattga
     3601 tagaaatgaa tagatcttca tttggatttt ctaatgaaaa ttgcaaatca attttttctg
     3661 ctagtccttg accttttgca tttaaagtca aaatagagat ttctggtaga tgaatctcag
     3721 ttggcaaaac agatttgccc tttatattta tagcacttat atcttcatta gaaatatttc
     3781 taccatttaa agctaatgaa tattctaaaa tattaggata tgttacattt ttcattgcaa
     3841 attgcaaatc tataatggat cctttaccta aaacaagttt gagcttatca gaatgcaaat
     3901 cattgacgta tccattaaca gatccatata atctaatagg tattataata ccttttaatt
     3961 caggagcaaa aaaactaata tctataaatg aaagattaga aggcctaaaa gtcatattca
     4021 tctctacaga atctacaaaa tattgaaaag attcgaatga tttatatcta aacaaaagat
     4081 ctgtattcaa ataagtttgt ggtgtgagta tgtgagcatt atttagtgca aaatgtttag
     4141 aagtcattgt aaaatcccca tcaaggtgtt ttatttgaaa gccatatttt tcttttgctg
     4201 ataaattgtg tatttttaca ctgatatcat tatcgtccat taattgaaaa tcagagatcc
     4261 atagattgag gtcaaaaaac tcaatagcat cataattaaa agtactgtca gagggatgcg
     4321 tattttcatc tacatatttt actattccat ttgatatttt agaattatta ataaaaatat
     4381 gtggtgttga tttttctttt tcattagcaa aaaattcaaa aataaaattc atatttaatg
     4441 tagtatcacc tttatatatc ctaagattca catatggatc cctaattata gcatctttaa
     4501 attccatttt aaaaggttta gaaaaaggag ctatattttt aattttagct tttaatgaag
     4561 atacagctat caaagtacta tcaagttgat catagagttt gacatcgatt aattctatgt
     4621 tagtgaacgt tgctattttt atagcaccta tctctaattt agcattcaaa ttgttctgcc
     4681 accaattttc aaaaatttga attaaaaatt tttgagttgt aggtgtttta agagtaatga
     4741 taagtatggc tggcactgca catattatcc atgccagcac taatattgta tttttaaaaa
     4801 tttttttacc tttgtgtaaa gacataatta tgagtttaat agtaatgggg atagaaacaa
     4861 gttgcgatga gacttctgta gcaattttgc atgatgacac tcttattagc aatataacag
     4921 caacccagga tgtacatgca tcctacggcg gcgtagtacc agagctggca tctagagctc
     4981 atacccaaaa tattatacct gtaatacata cagctcttca atatgcaaat ttacataaaa
     5041 aagatgtaaa tgtagtagct tttactttag gtccaggact tgttggttct ttaatggtag
     5101 gcattagttt tgcaaaatct tttgctcaag ccctaaatct acccatagta gctatagatc
     5161 atttatatgc acatgttttt gcttctttca tacagtttcc aaatgaaaaa catgaagtac
     5221 ctagttttcc ttttttgtgt ttgacagcat ctggaggaca tacacaatta cttagagtaa
     5281 aagattttac tgatatagaa atcataggac aaacaataga tgatgcagca ggtgaagctt
     5341 ttgataaagc tgctaaaata ttaggattac catatcctgg cggtcctcta atagatgaat
     5401 tagctaaaaa aggaaatcca ttttattttt cttttccaaa acctaaagtt gcagatttcc
     5461 aatttagctt tagtggattg aaaacttcac tattatacct tgtcagagat aatcttaaag
     5521 atgatccagg atttataaat aaatataaag aacatttggc agctagctat caaagaacaa
     5581 taatagaaat attaacagaa aaaacattta aagctgcaga aacttttggt ttcaaaaaca
     5641 tagtcctggc cggtggcgta tctgctaatt cagaattgag aaaaacattt atagataaag
     5701 gtaaagagct tggatacaaa atctatattc ctcctttgag cttgacaacc gataatggag
     5761 caatgatagc ttttttaggt aaaattttat atgagagagg cattaccagt agtctagatg
     5821 taaaacaata tgcaacaggt gaaaaatata aaaaatatca cagcaaaggt taaataaaag
     5881 aatgagcaaa tagaaaatac gaagtccaaa aaaaggtttt tatgtagtga atttgatgaa
     5941 attaataaat gtttaagttt ttcaagttta tatgttaaat aatttttata tttttacaaa
     6001 aattttaggc tatgaaaaaa attgtagaaa acaccagcaa atacctcgct atgagtaagg
     6061 aagagaataa agaattctta gacaacctgt attcattaat gaaaaaatta gaagccgatg
     6121 tagtcgatta tcaaggtctg aaaatactca gcgccccgcc actgtgccca gactgtcgta
     6181 gcaatcatat tattaaaaat ggtttacaaa atgggatgca aaactatcgt tgcaaagatt
     6241 gcggcagaca atttagagtt accactggaa cttttgttta caggttgcaa aagaaagaaa
     6301 aaatgctcga ttacatcaga tgtatggtag caggcaaaag tctcagagct tgtgctaaag
     6361 aggttggtat cagcttacca acaagcttcg cttggcgaca caaaatattg gcagctctac
     6421 aaagtttcga agataatata aattttttcg gagtagtaga gatggatgaa ctattgatgg
     6481 attattccga aaaaggtaga atatacaaaa atgcagaaga gctgaagcga gctaggaagc
     6541 aaaagccaaa gaaagtagct gtattggctg ctacagacag agctggcaat ttattattca
     6601 aaaaatttga aagcaaaaag ctaagttcag aacagataga gcaatttctg aaggctaaga
     6661 tgccaaaaga tggagtcatg tgtagcagta ataaaaaagc attcaaaaag gttagaagga
     6721 atgtaatcaa gcacaaagaa atagtaacta gccataggca aaagcgaggc atatatagcc
     6781 taagtactgt caagaaaaag gctagtgaat tcacgaattg gatatatttc aaatttaggg
     6841 gcgtagctac caaatacctg caaaactaca ttatgtggta tgtagtgatg ggtaaatacc
     6901 tcacaggcaa gactgtaagc aatactgggc gaatgctcaa ccttgcatcc tcggacaggt
     6961 gggcatggta tcggtataga gagttagtga atctaacata tgttgattga aacatatgtt
     7021 agatttccga tatacccaaa tcaatctctt tattagtgtt aaatttttta aattaaaaaa
     7081 ctatataaat tacattttaa tcatatatga ttaagaattc agagttttgg taaaaaatct
     7141 atagttcacc gatatagaaa tattggatgt tagtttttta tcttataatt tgttaagata
     7201 aaatttctaa aattttttta tgaattctat atttttttca caaaaacaag acttttattt
     7261 ataataataa attatccttc aatatataaa ttaaatcatt gagaaaagtg atcataaata
     7321 ataaaatatg tttatataaa tttaatttta catcttatta ttatgtttta aaaatcatat
     7381 ttattaaatc gtgagttttt gtttaattat tgttttacta ctttgatagt tttattagca
     7441 cctatagcca tgatatatat tcctgatgat agtgaatcta gttgttctat tattatataa
     7501 ttattgccgg gaaataattg atatatattt ttaaatactt gtgtaccatt aatagaataa
     7561 agagctatat ctagaaagct tttttcttta gattgatata taatatttat atatgatgta
     7621 aaaggatttg ggtatatgaa aggtgtagat tctagccatg gtggagaaga tatagaagta
     7681 tcattagcta agatatcagc tactgcgaaa ttgggaatac cataaccata aaagttattt
     7741 ggagttaaat atctattagc acttttaata atcgatgtga tgatgctaat aggatctgag
     7801 tttggatgtt tttgtagtaa gcaagctgta gcacctgcta ttataggtga actgaatgag
     7861 gtcccattac ccattattat gctgctatct tgagaagctg taaatacatt tgcaccttgt
     7921 gcacaaacag taggtttaat gcgtccatca taagtaggac ctttcgaaga aaattcaacg
     7981 acaattccta gtgagtccac agctcccaca gttatgatac tatcagcatc tgcaggtgct
     8041 cctatataat accaactact attgccacta ttgccagcac tattgcatat tatcatgcct
     8101 ttacttgcag ccatagctgc acctttgctc actcttgtag ttttgccatc aagatccgtg
     8161 tagctatgat tttgagaatt tagattaaaa gtcgtatagc ctaatgagga attaataata
     8221 tcgactccta cactatcagc aaattctgct cctgctatcc aattttcctc ttctatcaaa
     8281 tattcagaac tgggatcttc agtccttatc aaccaataag aagcctcagg ggctgtacct
     8341 actaatatat ttggtatatt gctagccatt agtgctaata cagaagtacc atgattgctt
     8401 ttattataaa cgttattgcc aggaaataca aaatctttgg ttcccaataa tctacttttt
     8461 aaacgtaaac tatcaaaaaa tggtatatga tcgcaacctg aaaaacctgc atctaacaca
     8521 gctatcacta taccgtctcc tttataaccc atctgatgca aaatgtgtat gccgatcatt
     8581 tggttttgat ttgttgagaa accataaatg gtttgtggaa ttttggttga tttttttgtt
     8641 atatcagaat aagctattga tggggtagtg attttagttt catcagtttc tttatttatt
     8701 tttggcttta ctaattgtgc atttactaca aatggtaatt gcaaaatcct attaataata
     8761 ttatcatttt gtatttttat tacagcagag ttaaaccatc gtgaagtata taggacttcg
     8821 caatctaaag atgttagact gtctaaatac caactattga caggtaaatc tgtgctatca
     8881 atgggaatat taaacttttc tcttcgttct atagctttat tagacaaaaa taactcaggt
     8941 tgatcgagtg aataattggt attaaattta ttagaaaaat ctactctgta gtatatctga
     9001 gaattaattt ttatcccaga taataaaatt aatattatta aaaaaattga tctattgcgt
     9061 accatattct attaatttcc attcatattt tacacctttt tttatctctc ctgataaatt
     9121 atctttattt tccaaatcta caaaaacttt ttttactaag ccgacatttt ttgcataaat
     9181 ctcatatttg tagcgaacat attttagatt tacaaagcta tcctgcaata ctttgactgt
     9241 agaatcaaaa taaaaattta aaatttgcaa tggatgatgt gcattctgat atttatattc
     9301 ttgatatcct agattattta aggcattacc atcccaccta taaaaatttt taataggaaa
     9361 aatcaatttg ataaagcgtg tgttttcttc tactctttct actcgttgat tagtttttac
     9421 gcagtaccac acatttttta atttccatgg caaagtatca gaatatttat gccatctctc
     9481 tatacgaaag gccatacgcc cttcattatc tacaaaagtc gattccatta attcttttag
     9541 taaaaaatta gtagtatcag aagtattata aaaatcatca aaacatatag aatcgactgt
     9601 ataaataagg taataaccag gtttggttat ataataatca taaccaagat ctatggtttc
     9661 tatatcgctt tggcaactaa ataaagcaaa aattaatata attaaaatga tgtggtgctt
     9721 catctaaatg tatcattaaa aaaatcactt tcagatggtt cttttattaa tccttttttg
     9781 tatagttcca tattttcata tatttcattc tctattttga ttggatcttt atcaggttga
     9841 taaacttttc tttctattaa tgttccttta taataggttt cagtaagtat tactactcca
     9901 tcttcatcat acatgatcca caagccatct cttagaccat ctaaatattt gcccatctca
     9961 tatatttgtc catcagaata aaaatattta gttagtccct gtatagtatc atttttatat
    10021 gttgtgatga ttctcggatt ttttgttggg tagaataatt tatattctcc atttctttta
    10081 ccattaatat aattagctat ctgtagagta tctccattag gaaaaaaatc atagcaaaag
    10141 ccattaagct ctccattttt ataattttct attgctatta tatgatcata tccatcgtaa
    10201 tatatccatt tgccttcttt ttttttatta taataaagcc cttcggcata ttttttacca
    10261 tttttataat aatgaatagc ataagctttt ttgccgttat cactgtatat gagcttgctt
    10321 tttaatattt tttctggata ataatatgta aactcgccga ttgggattcc gtgatcaaaa
    10381 tgcccttgat aaaaaagtct atcatcctta tatttttccc aataaccatg tggtaaacca
    10441 ttattatctc ttagatttat tgtatcattt tgacagatca attgattatt aatcgtaata
    10501 agcaaaagaa aaataaaaaa tggacatttc atgattataa attttgataa tacttatgaa
    10561 tttctaaagc aaaatcatat aaactattaa atacaaggtc tatatccatg attgtaagtt
    10621 gatcttcctc gaggtctcca ataaaaattt ttttagcacc gagattttga gcaaattcca
    10681 tatctatttt tgtatcacct accatcatac tatgtgaaaa atctataaca ggaaaatctt
    10741 ctttagcttt tagagccatg cctggattag gtttacgata ttgagaattt gatgatgcta
    10801 gatcagggca aaaatatata gcatctatgc gtccaccagc tcttagaatc tcttcgtgca
    10861 tatagttgtg tacctcttgt aaatcttttt cgctcattaa acctttgcca atcccttgtt
    10921 gattagttac tattattatt ttattaaaat atttatctaa aatggctaaa gcttctttag
    10981 tattgggaat aaattcaaat tcatcaatta tttttatata atctccgatt tttcttttat
    11041 ttattacacc atctctatct aaaaatagag tccaattttt atggatattc cattcagata
    11101 aaaccttttg attataagca gacattatta tttcaattta ttttctaaaa aatagcaaaa
    11161 tgcatgacct atagtgatat gagcctcttg tatatgtggg gtaaaatcac tcgggatacc
    11221 tagccatata tctaccaaat cttttagtct acctcctgtc tctccagtcc acccacttgt
    11281 tactaatcct atttctttgg ctttatttat gacttttatg atattaggcg aattgcccga
    11341 agtagatatg ccgaccaaaa tatcatatgg tttagctaat gctacaagct cccttacgaa
    11401 gacattgtca tagttatagt cattagctac cgctgtagta tatgaaaaat tagtacccaa
    11461 ggctatagct ggtataggtt ggcgatcatt taaaaattta ccggtgagtt ctgcaactat
    11521 atgttgagcg tcagcagcac taccaccatt gccacaaaaa aatattttac ctcctttact
    11581 tatattaatg atcgagattt ctattaagtt ctctatatta ttaattagct cgtcgtttgt
    11641 gatgattttt ttcttgactt ttatactgtt attaatgtaa ttttttatat cttccatttc
    11701 ttagaggttg attttttttt aatattcaac aaaaatataa aaaaaattaa agataaagat
    11761 aattttctca atcctagaaa aattaaaaat ttgtttaatt tcaaatcata tattaattta
    11821 gtaaaaatta ttttataatt atgaaaaaag tattagttat tttgtttgcc ttattatcga
    11881 ttaattttgt ttttgcacaa acaaaagaag taacatttcc tattaatgac aaacaaatat
    11941 ggcagattgt cgataatgag attatgtctt ttagagttaa aatttcattg ccagctattc
    12001 agttacaaaa tgtcaatacc ccacatggta tttttactca aatcattggt gatgcactag
    12061 ctattagtta tgatactggc aaagctcagc tacctacatt aaatcattta atagaaatgc
    12121 catccgaaaa tttaaatatt acaataattt ctaaaaaaga acaaatcata aatttgaatg
    12181 aacttggttt cacttcacct ataatacctg cacagcctag tatcagtaaa agtacggatc
    12241 ctaaaactgt tccattttat tttgatgaaa actattacaa taccaatgta tttcatagaa
    12301 aacaactggc agaaatagaa tattatggta aacaacgagg taccggtata ggattactaa
    12361 gaataaatcc tttcgcttat aatccagtta ccaacttatt agagattatt tatgaagtag
    12421 aattagaagt aacttttaat tctaataata taaaagatta tcttaatgat aaattaagat
    12481 tgttttctcc attttttaat ttttctaatt tactaaatta ccaaaataat gttataaaag
    12541 atgtgataac agattatcca attacttatc ttataatatc acctagatct tttgaaaata
    12601 ctttacaacc atttattcgt tggaaggcag aaaaaggtta caatgtagaa gtagcttata
    12661 ctgatgtaat aggaacaagc accacaaata ttaaatcata tttacaatct ttgtatagca
    12721 atactacgcc gcctcctact tttcttttat tagtaggcga tgtagatttg ataccttcgt
    12781 ttaatggtac aacacaaaat tcacacgtta cagatctgta ttatgcatgt tatgatggat
    12841 ctagtgatta tttgcctgat ttatattatg ctcgctttag tgctcagaat gctactcatt
    12901 tgcaaaattt aattaataaa actattactt atgagaaata tcaaatgcct attaaatctt
    12961 atctggatac agtattaatg gtagctggtg tagataataa ttattcatct acttatgcta
    13021 atggccaaat aaattatgca acccaaaatt attttaatag taataacggt atttacagtc
    13081 atacttatct atatccagca agtagtggta gtggtgtatc agccgatatg atttcaaaaa
    13141 taagcagagg tacaggttat gctaactata cagctcattg caattcagca ggttggggtg
    13201 atccatcttt tacaacttct aatgttgcct ctttacaaaa tactcataaa tatggattga
    13261 tggtaggtaa ttgttgtcaa tcttctaaat ttgaaataaa tgaatgcttt ggtgaagcta
    13321 taacgcgtgc aactaataaa ggagcagtag catatattgg tgcttctgat tattcttatt
    13381 gggatgaaga ttattattgg ggagtaggat atagaagtaa tattgtagct aatcccacgt
    13441 ataatagtac aaacttagga gcttatgata gagtttttca tactcatggc gagccatatt
    13501 ctgagtggtt tataactaat gcccaaatga tacatggtgg taatttagca gttcaatcat
    13561 caaattctag tttgaaaaaa tattattggg aagtatatca tttattcggt gatccatcta
    13621 cgatgaatta tttctcagtg cctgatccac ttactatatc ttataatgaa ccacttataa
    13681 taggagataa ttctcttatg gtagagactg aaccatatac tttagttgca ttatcatcga
    13741 atggtatttt attagatgcg caatatacat ataatagcaa ttctgttaat ttaactttcc
    13801 aagatttagc tactgatgat agtttgttaa tagtagctac caaacaaaat aaagtccctt
    13861 atattgaaaa aatagaagtt aatgaaaatt tacttgcatt agatgctaaa atttctgaat
    13921 tattagcccc tgtctcttct tataattgtt atgatataga tataactcca agagttacaa
    13981 ttcagaatct tagtacgcaa aatttaattt ctctaaatgt ttgttattca tatgcagatc
    14041 aaccgctgca atgtgtcaat tggaatggta atttagcttc tttagaaaaa gacaccttag
    14101 atctaccaag tttacattta ttagaaggcg ataatcaatt tattgtattt tgtgaaaatc
    14161 ctaacaatgc cagtgatatg aagccgagta atgatactat ctcgataaat gtaaaatcgg
    14221 aatacaaaaa tataatctcc aatttcgaag ccaatgtgac ttctggttgt aatttaccat
    14281 ttatagtgaa tttttctaat ttatctcaaa atgctctaag ctatttatgg gattttggcg
    14341 atggctatac aagcactgat tatgaacctt ctcacgtata tacttcttat ggtttatata
    14401 ctgttaattt gatagctgat gctggagttt gtggtagcta cacagaaact aaagttaatt
    14461 atatacaagt gggtagtttg ccgcccacag tccacgatac tactatatgc ataggtgaag
    14521 atgtcatttt agaagcgtat gctgatggtt tagttaattg gtatattaat atgtatgacg
    14581 tcagccctat atttacaggt aattattttt ctatttcaaa tctaacacat gatactacat
    14641 tgtgggtaga taattatgta gaatccccaa ttcaacatgt gggtagcact caatcaaata
    14701 ctaatggtgg tttctttaat tcgtggacta ctcattattt aattttcgat tgttatcaac
    14761 ctgttacatt gatttctgtg gaagtaaatg ctaaaactga tgggaatcgt accatttctt
    14821 tgcgcgatga aaatgataat actatacaaa gtactacgat aaatatccct gctggcatta
    14881 gtcgcgttac tcttaatttt gatttaccag tgcaaaacaa tttgcaatta tcttgtacta
    14941 cttttccaaa tttatatcga aatagttcgg gtgctcaata tccttatgaa atttctaata
    15001 tgatttctat aaaaggtaat tcagcaggag atcctgatta ttattattat ttttatgatt
    15061 gggaaatcaa aggtgtagat tgtataagcg agcgtatacc tataacagct cacgtagata
    15121 attgtgatga gataaagttt tttatctcta atgaatttat gatatatcca aatcccgcct
    15181 ctgattttat aactattgaa ttaaacgaag ctatacctac taaattaaac atattctctc
    15241 tagatgggcg tcttttgaga gattattatt tttctaataa taaatttgta cttgatgttt
    15301 ctaatttgac tcctggcaca tatctattaa gactaagtaa tgataatggt acatcgcaaa
    15361 agatttttgg cgtttttcat tgataacact gagacaaaaa atcctcaatt ataactttct
    15421 tttttaaaaa aaatatttca attaattatt tttttaaaaa aataattttt gcataataaa
    15481 taattttatt atttttgcaa aaaagatggc gtcgtagctc aggcggtaga gcagaggact
    15541 gaaaatcctt gtgtcggtgg ttcaactcca cccgatgcca cttgataaag agggcgattt
    15601 attgtaataa atagccctct tttttaaatt aattgactgt caattaatag ttttcaaatt
    15661 atacataaat aatttttttt ataaaaaaaa tttgaaaatc attttattaa tattttgtaa
    15721 ttttgttttc tctggtttat tatattattt taatagtgat attataatat taaagctaga
    15781 aatatgataa aatcataagg ccccgtagtt caatggatag aacggaagtt tcctaaactt
    15841 tagatgcagg ttcgattcct gccggggtca ctaaaatatt aatgttttgg gttacactat
    15901 ttgtatagat ttcggaaata ctagcacaaa ggtatctcta aatgatgcta ttttctcatt
    15961 gtctacagat gaatatatag aaataaatat taaaaatatt ttaaaaaaat ttgatattac
    16021 acatggatta tattcttcag taataacatt gtctgatgga ttgcaagatt tattagaaaa
    16081 aaataatgta ttatctatta aatctaaata ctctaatttg ccattttcaa tagattatca
    16141 gacaatagat actctgggtg aagatagaat tgctgcatca gtaggtgctt atagtttgaa
    16201 aaaaaataaa acttttttaa ctattcaggt gggtacagct ataacctatg attatattat
    16261 aaaaaagact tatgtaggtg gtgctataag tcttggtttt gatataagat ataaagcatt
    16321 acataatttt acaaaaaaat taccgttatt aacaagtgaa gataataaaa atattaaaac
    16381 tcctatgata ggtaataata caaatgatag tatccactct ggtgtttatt ggggtgtaat
    16441 ttatgaatta caagctagaa tagatgaatt tataaaaaaa tataaaggac ctgtatattt
    16501 gactacttcg cacaaagaaa atatatattt aaaaaattgc aattttgccg agcaaaattt
    16561 agtgctttta ggactaaaat atttactaaa atgaaatttt ttaagaatat ttattttgta
    16621 ataatttttt ttatgctcat tactactttt attatggggc aagataattt ttataatcca
    16681 tattctagat atggtatagg agatttgcag aatactaatc actcattaat ttatggatta
    16741 gggataaatg gaggtacttt ttatgataaa agcattataa attatttaaa cccagctacg
    16801 tattcggttt ttgactcatc atcagttgta attaatatcg gtgctaatgc gataggcaat
    16861 attttagaaa attctaaaca aagttcgtat aaatggggtg gagggctctc gatggttggt
    16921 ggtggtttta gaattaataa atttttatct acagctattg ggttagtgcc tttttctaat
    16981 gtaggttaca aaattgtaga ttctatgccg cagtttacat catcttcgta taataacgtt
    17041 tacagttctc atggtggtat caatcaagca tttttaggat ttggaatcaa tattaccaaa
    17101 aatttttctc ttggtattaa ttttagttat ctctttggtt ctatatatca aagtaaatgt
    17161 actgattttg atagcactta tttttttgac aatagatata taaatgaaac ttttgttaat
    17221 gatttttact ttaatgctgg tttgcattat ttgataccta tgcaaaagtc taatctttca
    17281 tttagcttta cttatgcacc agagttatct atcgatgcta gtagaactat tagatctgag
    17341 ctcgttacat ttggtattta taatgacatt ggtatagata ctactgctga gtcaaaaata
    17401 aaaggcaaat atgagtttcc agcattctat tgcgctggta ttagctggga taataaagaa
    17461 aatatcaaaa tatttattga tgggtatttt tcgaaatgga aaaacttcaa aaatttcgga
    17521 gaaactgata gcttacaaaa tagttttgct atacagtctg gtatttcatt tatacctaaa
    17581 ccaaactcat taaaaaatat ttttcttaga agtaattata ttatcagctt taaatatgct
    17641 aaaacttatc tcaatttacg taacacttcc ttagacgcat atacattttc tacttctatg
    17701 ttgttgcctt ttcgccctat ttttaaatcc gtttcatcaa ttggcattaa ttttgcttac
    17761 acttatttag gaactaaaaa aaataattta atagctgtta ataactttaa tattggcata
    17821 tcgatacatt taaaagaagg tttttatgaa agaaaaaaat atgaataaaa aattaaaaaa
    17881 aataaaattg tgtggaataa aaaaataaaa aatatgaaaa ggttagtttt gattttagta
    17941 atttttatag gtataggagc tatgaatgct cagcaaacca aacaaatcac atttcccgaa
    18001 ccttgcagta taaatggacc taaatggggg ccagatagtg taaaagccgt gcaaagctat
    18061 tctatctata gggaaaatat gaaacaatgg aataataata aagaacgaac tgaattaata
    18121 gaatactcat tagatagttg gcgttatcta tttaataatg cacccttagc tagtgcatat
    18181 atatatatag atggtcagga tataatagag tatttaataa agaaaaacga atcaaatccc
    18241 gaattaaagc aaaaatatat tgatactttg atgatggtat ttgataaaag gatagaagct
    18301 tttggttgtg aatctcactc atctgagagt tttgtgttag gacgtaaagc tctagctatg
    18361 ctacagtata gaccagaaaa tattgatgag attttagcta cttttcgtcg tagcatagaa
    18421 ttgggaaaag aagatgcaga gccagctata gtttttactt attatgactt tttagtaaat
    18481 caagtcgtta atggcaaaat ggatactata gttatcttcg aagagtatga tagattgtca
    18541 gatttgatga ataaaagatt agtaaaacta tatgatgaat ataaaaataa tccagcagac
    18601 agtaatagat atatcagatc tatcaataat gtgaaggctg ctattataaa tatggataat
    18661 tttatggcac cttttgctaa ttgtgatatt attataaata tatatagtaa taaatttgag
    18721 caaaataaac aaaacataga ttttttgcgt agattaacaa atataatgga gaaaaaaggt
    18781 tgtgatgaca ataaattgta tttcaaggct gccgagcaat tgtatacact agatccatca
    18841 cctgcctcag ctatatctct ggcaaagtct tttataaaaa ccaagcgata ttctgatgct
    18901 ataaaatatg ctaatcaagc agcagaaaat tttcaagatc ctgacgataa attcaatgct
    18961 tacatggttt tagcagaagc atataggttg aatggtcaat attcagcagc taaaaatgct
    19021 gctattaaag cttctaacat aaaacctaat agtgggcagc catatatttt tattggagat
    19081 ctttatatga ttacatctgc tagctgtggt gacaatcctg taactaaaag agctggctat
    19141 tgggcagctt atgatcaata tattaaagct aaaagagatc ctgaatttgc taatatagct
    19201 tctcgtaaag cttctgaagc ttattcttat tttcctaaaa ctgaagatct atttttctat
    19261 ggatattaca aaggaaattc atatacaatt aattgctggt atacagagac caccactata
    19321 agagcctctg attaataata ttatgaggtt tttaagttgt ttgtttaagt tttttatagt
    19381 tttgttcttg tttttagcta ttttgggctg ccaaccaagt accttagaag agataaaaaa
    19441 agtaacagaa caggatactt ttcctatagc tactataaaa aatgcaagtg tttattattc
    19501 tgatagtgga cgagtaacta tgcatatatt agctcctata ataaaaaatt atattcatcc
    19561 taatgaagta acaattttag aaggagattt aaaaggtaat ttttatgatt ccataggtaa
    19621 tgtgcaatct atcatatatg ctaaatatgc taaattagat gataaaaatg ctaaattaga
    19681 acttcgaaaa gatgtcaaag tatttaatat aattgataat actgaatttt attctgaaga
    19741 attagtatgg aatcgcagat ctcatatcat aacgtctact aaatttatta ggattgtaac
    19801 tcccgacaaa ataatatggg gtgaaggatt tagctctgac gaggaactca aaaattatga
    19861 aattttgcat cctaaaggcg aattgtacat tgataatata tgattttcag atttattaat
    19921 ttatctcgtg tatatcgata aattattttg atatgtgcaa gaatttttca taaatttatt
    19981 gactttttat aattttgcaa aaaaaatgag tgaaatatta aatgatctta agggaagatt
    20041 ggcatctctg aggaggtttc tttgacatcg ataatataaa aaaagaaata gaatctcaag
    20101 agactaaaac ccaagagcca aatttttggg ataatccaaa agaagcagaa aagattttaa
    20161 aagaaatcag taataaaaaa aattttattg aaaaatataa tgaggtagct cgaaaaatcg
    20221 aagatttttc agtttatgat gaatttttta atgcaggcga aatctctgat actgagtatc
    20281 aaaagcagta tgaattagtt aaaaaagcat tagatagact cgaattttat aaaatgatgc
    20341 acgatgaagc cgataaaaat agtgcgataa ttacaattaa tagtggagct ggtggtactg
    20401 aaagtcaaga ttgggctcag atactaatga gaatgtatat cagatgggca gaaagacata
    20461 attttaaagt aatagaatta gatttacaag aaggtgatgt agctggtatc aagtcatgca
    20521 ctcttgagat agatggtgac tatgcttatg gatatctaaa gagtgaaagc ggagtacata
    20581 gactagtacg tatatctcca tttgattcta atcaacgtcg gcatacatct tttgcttcag
    20641 tatttgctta tccacttata gatgagacta ttgatataga gataaatcca gcagacttag
    20701 aatgggatac atttagaagc tctggcccag gtgggcaaaa tgtaaataaa gtagagactg
    20761 ctgtaagagt acgtcacata cctacaggta tagtggtaga gtgccaagtc actcgttctc
    20821 agcagcaaaa tagagaaaaa gctatgcaat tgctcaaatc tcgtttgtat gaaatggaat
    20881 taagaaaaaa acaggaggaa ttagataaat tagaaaaaaa taaaaagaaa atagagtggg
    20941 ggagtcagat tagaagctac atactacatc catataaaat ggtaaaagat catcgtacag
    21001 gaatagaaac ttctaatgtt aatgcagtac tagatggcga aatcgatgat tttattcata
    21061 cttatttgct ttctcttaat ataaattttt aaatttttta ttaggtttgc aaataaaatt
    21121 ataaatttat ggaaaaagaa gaatttcgtt tacctgaaaa tgcttatcga gagctgaatg
    21181 aaggtgaaga atataagcca atactagatc ctacaaaaaa atataaagaa gtaactactt
    21241 ggtctgtagc aatgggtata ttgatggcta taatattttc agcagcagca gcatattctg
    21301 gcttaaaaat tggtcaagtt ttcgaagccg ctatccctat atctataatt gccgttgggg
    21361 ctacgatggc tgctagaaaa tccgatcctt tgagtcaaaa tgttattata caatctatag
    21421 gtgctagctc aggagtagtg gtagcaggag ctgtctttac tataccagct ctttatatac
    21481 ttcagcataa gtatccaagt atagaaatta atttctggcc aatatttttc tcatcgcttt
    21541 tgggagggtt tctcggtata ttgtttttga taccatttag aaaatatttt gttaaagata
    21601 tgcatggcaa attacctttt ccagaagcta aagctactac agaaatattg gtaacgggag
    21661 ctaatgtttc gaaacaatct tccatacttc taaaaggcgg tatcatcgca ggcatctatg
    21721 attttatgat caattcatta aaattatttc aagaagagtt tacatcaaga atggtggggt
    21781 ggggggctgc tattgctgat aaaactaaag tagttttcaa aatgaacgct tcggcattag
    21841 tatttagttt tggttattta gttggattga aatatgcatt gatcattgtt atgggttcat
    21901 tgctttcgtg gtgggtattt atacctatca taggttatat aggtggtatg caagatttat
    21961 cagcagaact aatattttta gattatgtcc gtccgatagg tataggagcc atagctatgg
    22021 ctggtattat aggtattata aattctttac caatattgag taagtctttt gggttattat
    22081 ttaataagaa gaaagtaagc gaaacagaag tattgcgtac tgacaaagat ataccttcta
    22141 gattttggtt atcagcattg atcttagtga tttttggtat tttactattt ttcatattaa
    22201 gtgtaaatct aaattggtgg caagcggtaa tagttttagt tgcactttta ataatttctt
    22261 ttttgtttac aactgtagca gccaatgcta tagctatagt aggtactaat cctgtatctg
    22321 gcatgactat gatgacttta atattaatgt cattggtatt agtaaaagta ggattaaaag
    22381 gtactactgg tatggtggta gccatgcttg tcggtggtat tgtttgtaca gctttgtcta
    22441 cagcaggtgg ttttgttact gatttgaaaa tcggttattg gattggttca tcaccatata
    22501 agcaagaaac attcaaattt ttaggaatat tagtaagtgc tgctacggta ggtatagtaa
    22561 tatttgtgct aaatcaagcc tatggttttg tgaaaactcc agaacaccct aatccattag
    22621 tagctccaca agctaatgct atggcttcta taatagaacc ttttatgcaa gaaaatgctg
    22681 aaataccatg gattttgtac ttagtaggtg ctattactgc tataattata aactttttgg
    22741 gtattcctcc attagcattt gctctaggta tgtttatccc tcaaggtctt aatacgcctc
    22801 ttttagtagg tggattaata aatcacttcg cagctaataa aactaaaaat aaagaattgg
    22861 ctaaagctcg tgaacaacga ggcactcttt tagcaagtgg attcatagca ggagctgctc
    22921 tctttggtgt tattggggct ctgttattat tctttggttt agaccttaca gttcccattt
    22981 ttaaggaatt agtaaatgga aaatgggtat ggatagatgg aaatgtttgg cctgaagtgc
    23041 tctcattagt agcttttgtt ttgatagtta catattttat atgggaaatt cgtagggcaa
    23101 aaatagaaga taattaattg gatttgagaa aagttgcgac gtttctcaat gactcttaaa
    23161 agataaaata ttttttaata ttttacctta gaataaaaat aaattgttat attttgattt
    23221 taaataaaaa atgtaaaata taatttatct ttgcaatata aattattttt attatgacta
    23281 aagacactaa atatattttt gtaacaggag gtgtagtctc tagccttggc aagggcatta
    23341 tatcagcttc tattggcaaa cttttacaat cgcgaggttt ttcgataact attcaaaaat
    23401 tagatcctta tataaatgta gatcccggta cattaaaccc ttatgaacac ggagaatgtt
    23461 atgttacaga agatggcgct gaaacagatc tcgatttagg tcattatgaa agatttctaa
    23521 acatttatac ttctcaggct aataatgtaa ctactggtaa gatatatttg aatgttatag
    23581 aaaaagaacg tagaggagat tatttgggtg aaactgtgca agtaattcca catatcactg
    23641 acgaaataaa atataggatt agattattag aatttagtgg agattatgat tttattatta
    23701 ctgaaatagg tggtacagta ggcgatatcg aatcattacc gtttatagaa gctgttagac
    23761 aattaagatt agagctagga aaaagatcac tcgtgatgca tatgacatta cttccttatc
    23821 tagcaaatat tggtgaatta aaaacaaaac ctactcaaca ttctgtaaaa aatttacaag
    23881 aattaggcgt gcagcctgac attttgatat gtagaacatc tgtacctata aatgattcta
    23941 tacgtcagaa attagctttg ttttgtaatg tcgaacaaag agctgttatt gaaaatattg
    24001 atatagacac tatctacaaa gtacctataa agatgcaaga ggaaaaatta gatgaagtga
    24061 tacttgataa atttggtatt tcatctacct ctcaaactaa tttaagtaaa tggatagaat
    24121 taattaatca tatagataat cctaaaaatg aagtcgatgt tgctctaata ggaaaatata
    24181 tagaactcaa agatgcttat aaatctataa ttgaatcatt tattcacgca ggagcttatt
    24241 tagaaacaaa agtaaatttg gattttatca gttctgaaaa tattactgaa ggtaatttgg
    24301 ataatttatt gaataaatat aatgctatat tagtagctcc aggatttggt gaacgaggct
    24361 tagaaggtaa aatattagct gctaagtatg cacgtgaaaa taaaattcct tttcttggca
    24421 tttgccttgg tatgcagatg gcagttgtag aatttgctcg caatgtcttg catttaaaag
    24481 atgccaatag tagagagata aatcctagaa cattaaatcc tgtcattgat ctcatggaaa
    24541 atcaaaaaaa gatatctgta aaaggtggta caatgagatt aggagcttac ccttgcaaaa
    24601 ttgttaaaaa ttctaaatta tatgaatgct acaaacaaga ccttgtttat gaaagacata
    24661 gacatcgtta tgagtttaat aataaatatc ttgaaatttt tgaatctcac ggtatgaaag
    24721 ctgtaggcat caatcccaca gagcaattag tagaagcaat ggagttagag gaccatccat
    24781 tttttattgg tgttcaattt catccagaat ataaaagtag atttgaatcc ccacatcctt
    24841 tgtttgtatc tttggttaga gctgcacttg agaaaaaaaa taatattata caataaatta
    24901 atctatcatt cataatcatt tatttttttt tattaatttt gtaaaaattt agtgttattg
    24961 tgaataaaga aacatcaaat aaaaggcccg agtggcggaa ctggtagacg cgttggtctc
    25021 aaaaaccaat gggcatttgc ctttgccggt tcgattccgg cctcgggtac taaaatggaa
    25081 atggtataat aatattaata atgtggtcat taatattata tcgaaaaaaa taagaaattt
    25141 atgaaaaaag cttatgtttt tccaggacaa ggagcccaat atccaggtat gggaaaagat
    25201 ttatttgatc attttcccat tgccaaggaa atgatggata gagctaatga aatattagga
    25261 tttaatataa taaaattaat gtttgagggc agtgaacaag atctaatgca gactaaagta
    25321 actcaaccag ctatattcat tcatagcgta gtattagcaa atgttttagc cgaacgtttc
    25381 gaccctcata tggtagctgg tcattctctt ggtgaatttt ctgctttagt agctgctgag
    25441 tctatgtctt atgaagatgg attaaagctc gtatatgcta gagctattgc tatgcaaaaa
    25501 gcttgtgaac agcaacctag taccatggca gcagttatag gattagatga taaaaaagta
    25561 gaagaggtat tatctactat aaaagatgaa atagtagtac ctgctaatta taataccgtt
    25621 ggacaaatag tgatatctgg cactttaaaa ggaatagaaa tagctatgga aaaactaaaa
    25681 gaagctggtg ctaaacgtgt tttacctcta aaagtaggcg gagcttttca tagcccactt
    25741 atggagcaag ctcgagctga actcgctact gctattgata aaactcctat aaaaatcccc
    25801 agatgtccaa tttatcaaaa tgttactgct atgcccacta ctgatccgaa tgagattaaa
    25861 aaaaacctta tcgatcaact gacttcacct gttaagtgga cacaaaccat taaaaatatg
    25921 attaatgatg gagcaacaga atttatagaa gtgggacctg gaaatgtatt acagggttta
    25981 attaaaaaaa tttcttcaga tgtgaatgct tatagtgcta ctatataaaa aataatatac
    26041 tatggatcta agtaaaataa tttacatacc tggcaaacca ggattatata aaataatagg
    26101 taataataaa acagcattta tagtcgaaag tctcatcgat agcaaacgat taccagtctt
    26161 tatgaatagc agaatgagtc cattatctga tattgttgtg gtaactaaag aagaccaagt
    26221 acatgtggag gtagttttta aaaatacttt aaaagaattt gatggtaaaa taattaatat
    26281 cgatcaaaat gatgaagact cgttaatctc ttttatggac aaagttttgc ctgattggga
    26341 caaagatgca gttaccccta aagatatcaa aaaattaata caatggtata atttattgat
    26401 agataaatct attatttcat tagaagactt aaaagaagat agtgtagaaa gccaagaaag
    26461 cgaagtaaaa gatgatagta tagcagatga aactcaaaaa gaaaaaagcg aaatctcgga
    26521 aaatgaagat caaaaacaat ctgataaaac agatgagata gaaataaatg aataataaaa
    26581 taatacgtac taagttaaaa gtagttaata atgagcaaat aaattcttcg cactttagat
    26641 tatatttaac aatgcctgat gattgtccac ctattacacc cggacaattt gccgaaatat
    26701 atgtgcctcc tacattaaat tcattcctac gtataccttt atctatacat ttcatttcgg
    26761 acaaaaaaaa cgaaattcag tttttaatac aattaaaagg tgaaggtaca aaatacttaa
    26821 aaactttgaa gaaaaatgat tttgtagaca taataatgcc tctgggtaaa ggtttttcaa
    26881 taccaagaca aagaaaactt gcactaatag ctggtggatg tggtgtagcg cctttattat
    26941 ttttaagtga taagctttat gaacatggta atgagttgga aatatattta ggttttagaa
    27001 atcataatca ggtaatgtta ataaatgagt tcaaaaaata tggcaaacta gtaatagcta
    27061 ctgatgatgg ctctatgggt gaaaaaggtg ttattactca agtatttgag cgccatttgg
    27121 atgattttga ctttatttat gcttgtgggc caactagtat gcttcaaagc cttatgtatc
    27181 tgagtgaaaa atataatata aaatgtgaaa tatcattaga aactttgatg gcttgtggat
    27241 taggagcttg tctatgctgt gctgtaccta ctaaaaaagg caatatgaga gcatgtgtag
    27301 atggaccagt attcgatact aaagatctta tagagctaaa tgaatgaagt gagttaaaaa
    27361 aaaatcttga atttcaaatt ttaagtgtaa ataatgagta caaaaataga tataggaaaa
    27421 ttgacattaa aaaatcctgt attaacagct agtgggacat ttggctatgg acaagagttt
    27481 gagagtttta tagatccttc acttttaggt ggtataataa tgaaagggac cacttgggag
    27541 cctcgagaag ggaatcccgc tcctagaatg gcagagacga gtgcaggcat gttaaatgca
    27601 gtcggcttac aaaacaaagg agtacaccat tttattgaca aaatactacc agctataact
    27661 aaatatgaca caaaaataat agtaaatgta gctggtaatg atgtagcaga ctatgtgaag
    27721 acagcatctt tattaaatga tatcgagcgt gtggatgcta tagagcttaa tgtatcatgt
    27781 cctaatgtga aaaaaggtgg tattcaattt ggtactaatg aaaatttatt aaaagaatta
    27841 cttagtgctg ttagacatgt atattataaa actttaatag tgaaactttc tcctaatgtg
    27901 acaaagatag aagatttcgc taaaatatca gagtcagaag gtgctgatgc tatcaccgta
    27961 gctaatactt ttttaggaat ggcaatagat attaatacat ataaacctaa attgtctaat
    28021 attacgggtg gtctgtctgg accagctatc aagcctatta ttatgcgtat ggtatatcaa
    28081 tgttatcaag tagtgaaaat accaataata gctagcgggg gtataatgca ttggcaagat
    28141 gctattgaat attttttagc tggagctact gctattcagg taggaactgc taattttata
    28201 aacccttctg cgagtataga aatattacaa gatatcaatg attatttaga taataataat
    28261 atagagtcta ttaaaaatat tattggtaaa gtaaaaattt agtggatgtc aatattaata
    28321 ataatcagct atactaacat aaatcttgtt aaaaaaaatt tattttaaaa agatattgtt
    28381 ttattaatag tgcaaataaa acttaagcta aatgaattga tgatggaggg atgcttgttt
    28441 ttgtgagaac gagaaaagtt tagtaactta tagaaagtaa taagttgaat atttgtaatt
    28501 ttcgtaaatt attatcaatg tttccctcta atttctattt ttatagagtt gtttttagta
    28561 aaatcaatat taatttaaca aatttgtaaa aaatataaaa agatgctacc aaaagaaata
    28621 aaagtaataa tctttgattt aggaagtgtt ttattaaaag ttgactatca aagaactatc
    28681 gattcattta aaaaattagg tatgacctac ccgaaacatt attttaatgt agaatataat
    28741 gatcctatat tagataaatt cgaaaaagga ttattatcag agaatgaatt tattaaaaaa
    28801 attgaaatga aaatttctaa agtttgctca aaaaaagaaa ttatagatgc atggaacgct
    28861 atgatagttg aatttccaga agaaaactgg ctattattaa atgagttaaa aacgaaatat
    28921 aaactttttt taatgagcaa tactaatatt atacattata attattgtca aaatttggta
    28981 caaaaaaaat ttggtgtttc ttctcttgat tgtatttttg ataaagctta ttattctttt
    29041 gttgttaata tgtgtaaacc agatataaat ttttttaaat taatattgga tgaacaaaaa
    29101 ttaattacaa aagaaataat cttttttgat gataataaag aaaatataaa aacagctgaa
    29161 aaattaggaa taaaatctat tagggtccaa ccaaatagtt cattaacaaa atattttgat
    29221 ttataactaa tgtatgacaa agtataattt atattttcta attttattca attgtttggc
    29281 atctacattt gtcggattaa attttttaaa atccaacgta agattaattt attggatttg
    29341 gcacttaatt aagtgacaag agaacaaact gtgagtagcc tcaatgagtc agcgacattt
    29401 cgttgtcata caaaccttga aaagcctgga tgctttcaaa acgattaggt catggttaat
    29461 atttgtctgg tgctgaaata aggttacagg ttaatattat taacacacca ttctatggag
    29521 caactgaagt tttatcaact aaatttaaac tgcctgtttt tttttgctga aatcataatt
    29581 gaaaaacatt ttgaatattg cagtaaatat caatatatga tggaaatgaa aagataagtg
    29641 atggtgagct gacacagaga tttgtaaaaa ttttagaaga agcctttaaa tgacactaag
    29701 cagattatct ctgatcgcat aaaaggtgga aataagttat aaaatattga tttattcttc
    29761 ttgtccatgt agagctttgt catagtctgg aggatcaagc atttcccagt atc
//
LOCUS       DAOPRN010000023        27340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1405_length_27340_cov_125.705443, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000023 DAOPRN010000000
VERSION     DAOPRN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27340
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1405_length_27340_cov_125.705443"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1581
                     /locus_tag="PKI65_04095"
     CDS             190..1581
                     /locus_tag="PKI65_04095"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOB77655.1"
                     /translation="MNWSLVIKRFVLASIVFLFLTSFLNGQNGTYRIHLLVIDLPEDQ
                     IFRLISIKGNALEPVDSFFSQNGAVDFVYSKPLPRGQYSITWQNNQKFIDFLFNNEHY
                     IELHTNHKDPIQNLKVISSEENKYFVKFLKIKDELYYLIDMGDEIYSKNPKDPLLGII
                     IDKIDSINNELSKFIKNVPEEFLAYKVIKANIHPSLEEFNKTHPNNRYNDIKSFYKRH
                     WFDNIDKQDSALVNTPVIYEAVKFYLQNLVEPPDTEQYKKAVDFILSQFSWNEQQYKY
                     VLNLLLNTFYTPQYESVFLYIYFNYMHDTQCSASVSDENYRKAMMITKLSKGAEAPEL
                     TGITLDSDSFRLSNITNKPVLLIFWAPDCYHCKSIMPYINNLYEKYGDDIYFVSFSID
                     TNVDAIKKATKEDSIKFTVITDLQGYNGENCLRWYIWGTPTFFIIDKNGKIYSSPYSF
                     EGLENDIKTVLLN"
     gene            1778..4312
                     /locus_tag="PKI65_04100"
     CDS             1778..4312
                     /locus_tag="PKI65_04100"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOB77656.1"
                     /translation="MSENKNKPKSYTYEEAIEASTQYFNGDQLAATVWVNKYALKDSK
                     GNIYELTPDDMHWRLAKELARIESKYPNPLSADMIYSLLKNFKYIVPQGGPMAGIGND
                     HQISSLSNCFVIGNQADSYGGIMKTDEEQVQLMKRRGGVGHDLSHIRPKGSPVKNSAL
                     TSTGVVPFMERYSNSTREVAQDGRRGALMLTISIKHPDAEDFIDAKADLSKVTAANIS
                     VKIDDEFMKCVIDDKPYVQQFPIDSPNPKVIKTIDARKLWQKIVHNAWKCAEPGVIFW
                     DTVLRESIPDSYADMSFRTTSTNPCGEIPLCPYDSCRLLAINLYSYVINPFSDNSYFD
                     FDLFKEHAAYAQRIMDDILDLELEKIDKIIAKIDADPEPTEIKRTERELWVKIKEKAI
                     QGRRTGIGITGEGDMLAALALQYGSDSAIDFSEEVHKTLALEVYKSSVKLAVERGPFP
                     IFDFKKEKDNPFLDRLFSIHPNLESEMAKYGRRNISMLTIAPTGSVSILTQTTSGIEP
                     VFSIFYKRRRKVNPNDKTVKVSFIDELGDAWEEYNVFHPKFLTWLEVKGYSIDEIKNN
                     FSDDKIDELINNSPYYHATSADVDWVAKVKMQGRIQKFVDHSISVTINLPENVEEELI
                     DTLYRTAWESGCKGVTVYREGSRTGVLVSSSRDIKEFGETHAPKRPKKLEADVIRFKN
                     EEEEWIAVVGLYNDKPYEIFTGRAEHLYIPKDITKGYVIREKDLGMEHARYDFVFYDK
                     DGYEVIIRGLSRTFNPEFWNYAKLISGILRHGMPLLKVIDLVDNLKFDNDSINTWKKG
                     VTRALKRYVPDGTKVEENCPQCGADDSLEYKEGCVVCSECGYSKCG"
     gene            4355..4963
                     /locus_tag="PKI65_04105"
     CDS             4355..4963
                     /locus_tag="PKI65_04105"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOB77657.1"
                     /translation="MKIIIPSLLLPPISFFIDALKADEWLIEINETFTKQTFRNRYYI
                     ATSQGIRAMIVPLYRPNGSNTLTKDILLSKDYNWIEYHHKTIITNYRNSAYFDYYEDE
                     LIDIFSKYDKLYEINTALLQWTLNQLNYKIDINYTNDFKNDYSNDYLDLRTKYRRYEA
                     IKIPVYRQVFIDKLDFIPNLSIFDLLCNLGPESIIYLKNLIL"
     gene            4960..5724
                     /locus_tag="PKI65_04110"
     CDS             4960..5724
                     /locus_tag="PKI65_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOB77658.1"
                     /translation="MKAYVTQSIIPMYRDPDYQSELINEILFGELIELNGSYGDWTKV
                     KCLFYDYEGWICSYAFDILNEDDDISKYKTITSITALIEIKDQIMCVSKGARIYDFMK
                     INIVDGKFRENNTKISVGNIIEDAQSLLFSPYRWGGKTPFGVDCSGFVQLVFYVNNII
                     LPRDAKEQSKIGEARIFAHDAYPGDLAFFGNIVYDEENQREIININHVGIVLNNSTII
                     HAFKSVHIDMFDHYGIYSENLKKYTHQLMFTKKVLE"
     gene            5907..6968
                     /gene="mtnA"
                     /locus_tag="PKI65_04115"
     CDS             5907..6968
                     /gene="mtnA"
                     /locus_tag="PKI65_04115"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOB77659.1"
                     /translation="MEINNKYYHSVWGDFKNHSINCIDQRKLPFIFEILELKTMDDLS
                     NAISTMAIRGAPAIGIAAAYGIWLSHLLRDGNKNLIKEDYKKLINLRPTAVNLKRGAD
                     FVYSNIKDHITNERVYKCADQFAKQEINACYQIGVHGVELIKNIYKTTNKQVNILTHC
                     NAGWLACGDYGTALAPLFEAQKQGIPIHVWVDETRPLNQGSRLTAWELDNANIPFTII
                     ADNTGALLMMNNKVEIVIVGADRIAKNGDVANKIGTYLKALAAKQHNIPFIVAAPSST
                     FDFNITDGQQIPIEERRGKEMQSIQVFYDNKLISAELFPVHYTSANYAFDITPHDFVS
                     NYITENGIFDFIDQVNKKI"
     gene            7014..7637
                     /locus_tag="PKI65_04120"
     CDS             7014..7637
                     /locus_tag="PKI65_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOB77660.1"
                     /translation="MNKYKKHAKELADFMRRLYQQKLTTTLGGNISMKVDDGILITPS
                     QIDKYSLKAYDIIHLSMDKKDIKSLNNASIETRMHLAIYKVRSDVGAIIHAHPFWCTV
                     ASIWRLKVDTNLTDEAYYNIKNIAYAKYFPMGSKELANEVADKSKSSDVVMMANHGIV
                     CMGKSLVKTFEKIEIIENLCHISWLKQFNDKITCLTDEAKNYINENY"
     gene            7772..8314
                     /locus_tag="PKI65_04125"
     CDS             7772..8314
                     /locus_tag="PKI65_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77661.1"
                     /translation="MKKFYFFIILNIFMWNSIYSQNYLTKIKRNLIEYQVSDSLKKML
                     IDYIINDFSKYSDSLIYFLIDANGYKYLGSSSTNTDTCVIHIGFITEILPSIEKEIKG
                     FFKLNNKLFFVINGHKDFLYPTDSIKEVEYMRVKSHKDNPLLEFLFYEDDMPFWLFDC
                     KDGEFYLIDYWKNYNFPEWK"
     gene            8305..8682
                     /locus_tag="PKI65_04130"
     CDS             8305..8682
                     /locus_tag="PKI65_04130"
                     /inference="COORDINATES: protein motif:HMM:NF026995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB-like toxin 1 domain-containing protein"
                     /protein_id="HOB77662.1"
                     /translation="MEVILFEFFANKTDVEWSHAMTGIAGDKGLNFITTGHDKGVEPG
                     MTALLTGQLHYGYTAREFNHSHPQNTPYPSGISGFTGETGDVQWAGEVCKIFGNNVKF
                     NIYTPKNGKYIQFSPNSKKSDYF"
     gene            8806..9210
                     /locus_tag="PKI65_04135"
     CDS             8806..9210
                     /locus_tag="PKI65_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77663.1"
                     /translation="MNEYIIVDTTIYNDLYDIILPEYNLFNKNEYNAFFINYHINSKK
                     FNLSMIYAPISDNLDTSIYIGFFYIKDKIFIFDKPFKEFIDCLEITNNKKEFIKFEKK
                     KSKDIINKIIVDYPLWLMEYKNGKIYLIEKYI"
     gene            9220..10092
                     /locus_tag="PKI65_04140"
     CDS             9220..10092
                     /locus_tag="PKI65_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOB77664.1"
                     /translation="MYHYHSDHLGSASFVTDAEGAVVQHLQYLPYGELFVSQRNTEKF
                     DSRYKFTAKELDNETSYTYFGARYYDSELSGWLSVDPMSDKYPSLSPYCYSADNPVVL
                     VDPNGEELNTYDGPPPKVITNNKEAIKHYYNGNGEPVKIGENITNELLKTKEFKEYHN
                     KIISGEVENKGYFNVNMEKEKKSFFIGHTNISYKVTSSEDNKNCTVTYTIYTNDGFWD
                     PDYIKENMGNKMAFDINITLLSAKEIFGIDIGYINTEKFKPDGLGPNLELGGTPYHFI
                     ETIIQYTFENPGYY"
     gene            10096..10674
                     /locus_tag="PKI65_04145"
     CDS             10096..10674
                     /locus_tag="PKI65_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77665.1"
                     /translation="MISKKLLLTIVFGTLIIASASLYIIADSDLYYYGKNNLPIYNKL
                     PFEMKPEYWGYRRGMLNFVILDKNDFVHIGRGVGYWEYPEITIDSVISYGYNDTLLIA
                     IVKDSCKQKYCLRQYDTLSEELIPLQYCNIEKLKHDYNLKWIENPNNPPYLIMSKRFR
                     SAFIFYISLILFIVYFSKYLKEKHKEKNKNIH"
     gene            10697..11572
                     /locus_tag="PKI65_04150"
     CDS             10697..11572
                     /locus_tag="PKI65_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOB77666.1"
                     /translation="MKIHYHSDHLGSASFVTDIGGNAVQHLQYLPYGELFVSQRKSKE
                     FDSRYKFTAKELDNETSYTYFGARYYDSELSGWLSVDPMSDKYPSLSPYCYTADNPVV
                     LVDPNLMELNTYDDPPPKVITNNKEAIKHYYNGNGEPVKIGENITNELLNTKEFKEYH
                     NKIISGNASTPGNFGINMENKKDAFFIGNTRVDYDITTSKDNKTCTVTYTIYTNDGFW
                     DPNYIKENMGNKMASDINLTLLSAKEIFGIDIGYINTEKFKPDGLGPNLELGGTPYHF
                     IETIIQYTFENPGYY"
     gene            11576..12160
                     /locus_tag="PKI65_04155"
     CDS             11576..12160
                     /locus_tag="PKI65_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77667.1"
                     /translation="MISKKLLLTIVFGTLIIASASLYIIADSDLYYYGKNNLPIYNKL
                     PFEMKPEYWNTRQGGPGFNIMDKYNFVHIGNYVGYREYPGIIIDSVISYGFNDTLIVA
                     IIADSSKNLYCFIQNDTLIRSITLIPLTNCNIEKIKHDYNLKWIENPNNPPYLIMSKR
                     FRSAFIFYISLILFIVYFSKYLKEKHKEKNNNIH"
     gene            12185..13006
                     /locus_tag="PKI65_04160"
     CDS             12185..13006
                     /locus_tag="PKI65_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOB77668.1"
                     /translation="MITAKELDNETSYTYFGARYYDSELSGWLSVDPLSDKYPGLSPY
                     IYSADNSVVLVDPNGASINPIYDIETSEFLGTDDKGLQGEAILMNKTDFKQGMSHEEA
                     MSKGKTLDNMSFDEALEFANNGKFKDFIDNYNNLPNRPDWDGYLTLNEANEWYRNGNG
                     QPLFVDLSKIDLSNLYSLGDNYIGEEKYINLFFASISTNDALVYGTIKLKRYPNDQVK
                     AYADVYDFNMHNSKNPLNWPRNIEAKIGKAYAGDGNPFNINFYGSKQLKKLPWIK"
     gene            12994..13416
                     /locus_tag="PKI65_04165"
     CDS             12994..13416
                     /locus_tag="PKI65_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77669.1"
                     /translation="MDKIIFKLKNVLVLIIFYLIIIFNLNCTSDYSKNLGSGYIYRDE
                     GCEEKEIFCEQANGGKIPKTVLVYDYDRHYIIAKQKPQKDDSNILLDDTIYEYKYGYD
                     TLYYWIILKDFKKVIGPLTEDEFLKAIIKYKVSKTLKL"
     gene            13413..13943
                     /locus_tag="PKI65_04170"
     CDS             13413..13943
                     /locus_tag="PKI65_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77670.1"
                     /translation="MKYITCCIVLLLNVNFNLFSQNRECDSTCTNDAIYIKTNEYEGV
                     ILGPKCLKRLLIKDGDTIDAPRWTPTMQDIENAEKLIRKYVTKKSKHHLVNQSDGCPI
                     IYQNFDKYVRQYYGIFNKKGQKILEVNFLWRDSKFIEEWKIKGITILDGCSYFWHISV
                     NMKKKKCFNYSVNGIG"
     gene            13992..14864
                     /locus_tag="PKI65_04175"
     CDS             13992..14864
                     /locus_tag="PKI65_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOB77671.1"
                     /translation="MFDTRYKFTAKELDNETSYTYFGARYYDSELSGWLNVDPMSDKY
                     PSLSPYCYSANNPVVLVDPNGMDWYDFTDENGNYSQLWREGNAATIVVNGDTYQNIGT
                     TNTIRINKNVEITYTQNEATSMTFIGIESDNWESQITNGTNCYEASCQMLNNEGVQTA
                     GRANEVLVTGLGENGRAGNPTANAQNGFKMIDNALEHGDPIIVGVDYQGGSPNYDKMT
                     DHFIVISSKTETLDKGKVTSTTYNYFDPRTKHKDWGTSPTNKLSIQNNKMVGTFNHEY
                     YNFNYTVTTIRPNR"
     gene            14885..15526
                     /locus_tag="PKI65_04180"
     CDS             14885..15526
                     /locus_tag="PKI65_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77672.1"
                     /translation="MKKILVYFIIGFSLLSCKGQNEEFNDYISNFQEFKLPFLIDRRN
                     TQYRLSTNEDYEIPANYIKKYICKDSTHCDYDQSKYRYTYGFKFKSKYTFVFLLKLTY
                     GGKTCYDFDLIETLLIVYSKDGKIIDAKSVAKDNDCWQSSIWATEDSIRVQQIKVTEL
                     DDFDEINKELDCEIETKIYQIGDDGYINLIKTEPIKRGVLIFDKNIDDFILKQ"
     gene            15626..15805
                     /locus_tag="PKI65_04185"
     CDS             15626..15805
                     /locus_tag="PKI65_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77673.1"
                     /translation="MGRAGQRSFFNYPVRQSLTPLRRRGVTSYSPLVEGMSHRDRGVK
                     TSGDLSFVSFSRRIG"
     gene            16122..17321
                     /locus_tag="PKI65_04190"
     CDS             16122..17321
                     /locus_tag="PKI65_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOB77674.1"
                     /translation="MRQSNLQYFVSQYTEPQKVQAEGIYPYFRILKSGQYPLVEYDGR
                     QILMFGSNSYLGLTNHPRIKEAAKKAIDKYGTGTAGSRFLNGTIDLHLELEEKLADFV
                     NKESALVFSTGFQTNLGVISSITRRKDYLIIDESSHASIYEGSRLSFSKVLKFKHNNM
                     QSLEEILSRIDNDISDTIKLIIVDGVFSMEGDIADLPNINKIAHKHNASIMVDDAHGL
                     GVMGEKGRGTVHHFGMDDDVELIMGTFSKALSTVGGFIAGSKEAINYLKHNARTIIFS
                     ASLPPASTAAVIETLNIINEMPEMIEKLWDNTHYAQRLLREAGFDIGNSQTPIIPIYI
                     RDDNLTFLYTMKLFDAGVFVNPVIPPAVKEGHSLIRFSMMATHQNEHIEKGIDICAKV
                     AKDLGII"
     gene            17371..18498
                     /locus_tag="PKI65_04195"
     CDS             17371..18498
                     /locus_tag="PKI65_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77675.1"
                     /translation="MINIISVTTKKQLRAFIDFPHDLYEGDPYYVPELYISQRDLLNP
                     NKHPFHKHAKVHYFLALNDNKVVGRLSAHINYSHNEYYKNKEGFFGFFDCINDREVAY
                     NLFKTAENWLKDEGMDTIVGPANFSTNESVGLLIDGYDRSPVVMMPYNYPYYQELIEH
                     YGFQKKIDLFAYWLEAEPISNKILDIGKKTEERLKNNGFTIRNIRFRTHFKEEVEKVY
                     EIYNKAWSKNDGFVPMTREEFFYSAKDLAMIAKKDFSFIATYKDKPVGFVVTIPDVNQ
                     AFKHLKRGRLLPFGFIKLLYYFNKIDTYRVILFGIIEEYRKKGIDVIFYLKSLETAKK
                     RNIFQGEASWILENNIQMNNILESIGAKKYKTYRIFQKPIL"
     gene            18495..19481
                     /locus_tag="PKI65_04200"
     CDS             18495..19481
                     /locus_tag="PKI65_04200"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOB77676.1"
                     /translation="MNEKVLVTGGSGFIGSRLIKKLVDLDYQVTAILRKTSKREHLPL
                     DDIDVVEIDLLDYHGILDYFTLHRFDYVIHTAGITKALKKEDYYIHNTKITKHLIDAL
                     LKISWKPQKFIFISSQASHGTYKALQRPIKITDIQNPLTTYGKSKLLAERYIEEHYHN
                     PFVIIKPTSVYGPRDKDFLIVMKLISKGFEGYIGTEPQKLSFIYVDDLVNAIISTIAL
                     PYTGEFLLSDGKKYIDEDLGRYIKKALGKKTIKIRVPIAIAYIAAFFSDLLARIIGKP
                     TAFNLEKMKELKAKNWLCDPTPFFETFHFIPCYDLQKGIKETVEWYKENGWL"
     gene            complement(19541..20839)
                     /locus_tag="PKI65_04205"
     CDS             complement(19541..20839)
                     /locus_tag="PKI65_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB77677.1"
                     /translation="MRSTKYLFVLIFITFNINLNGQHKTLIENYLQKIDTFKYVDYNE
                     IYDYAIAAYNLSLQTKDTFYLGRTAKELGAYYIFKGKQDSALYYTNQAFKFSNTAKDS
                     LNLSLTANNLGVIYQNMANYDSAYKYFMISYNINIATDNKKELIADFNNLGMLYYVMN
                     NYPKAIENFTIFLNMALENNDSTLITEAYINLALTFNQLDKYNYALDFLNKSLRYTGK
                     NIYKKVKILDNLGITYAYLENYKKAKEVLDISYKIKLKYGFLSELIQSHLYYAEYFAM
                     LSEFDSLESHLADAMQLCFETKNLRLTYYTLTMYGLFFREMGFIDKAIAYLINAEEIA
                     TNAKAYNYLLEIYQNLADLYVSVENYQKAYEYQKKYTDLLLVNDTIREISPPISNEEL
                     DNVNQATVFKNNNFYLFLILGILMAITTLLIINLIYKNKH"
     gene            complement(20929..21201)
                     /locus_tag="PKI65_04210"
     CDS             complement(20929..21201)
                     /locus_tag="PKI65_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOB77678.1"
                     /translation="MNKKELIDAMAKEAQLPKNKTEAALNAFMKVVNDAMKRNERVTL
                     VGFGTFEVANRKARNGVNPRTGEKIIIPAKKVVKFKVGSQLAETVK"
     gene            21608..22735
                     /locus_tag="PKI65_04215"
     CDS             21608..22735
                     /locus_tag="PKI65_04215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOB77679.1"
                     /translation="MKILQVCGGYSMGGLEQQTIKIAKGLINKGHELMIYCSKDSLIS
                     SKCKTENINYSEGLFDIGNNVFKNFKNTYKILKKFKPDIIHVQRSHDLFPIVLVLYIY
                     KINTPLVFTRRMESSINKKTFFHKLIYKRINKAYAISSFIKDNLIATTPIKPDKVEVL
                     NNGVDLKIFNPLNYDKKTCRKKYDMPLDATIIGMVGRISPMKGHKEFVRAASELFNNR
                     NDKLFFAIIGGASVGEESFAQEIYQLAKTLLPSSNFRFYEFTDSVAEALATMDIFIFP
                     SYRESFGNALLEAMAMQLPIVTTYAGGVKDIIICGENALCIEPKNHLQLVNAVNQLLN
                     NEELKIKISIKARKTAEKHNFENYINKLENDYLDLIKKLNF"
     gene            complement(22782..24377)
                     /locus_tag="PKI65_04220"
     CDS             complement(22782..24377)
                     /locus_tag="PKI65_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOB77680.1"
                     /translation="MNKADWIFYDGNIYTVNDSFEKVEAIVIKDDKIVDVGDSDTLLK
                     RWNSKEKINLKGFTIIPGIIDAHCHLISYSTEMEPIDLTGVRGVDEMIERIQIYKKQH
                     PDVTFIYGRGWDQNLFPNMQMPNNKLLNIYFPDIPVYLKRVDAHAALANDAALKLANI
                     NVDTKVENGIIEVKNEELTGILIDKAMELVESHIPHLPKSAKISSLLKLQEQALSYGI
                     TAINEAGINSEDIYLLDSLQSEDKWVLRVFGMLTPNDDNIENILKNGVYKNNKIIVNS
                     VKLYADGALGSRSAYLYEPYSDDHNNRGLLLFDEYNFNYIAELCIKYKYQIATHAIGD
                     AAVGTILDWYSKYLTPENDLRWRIEHSQLVNPMDLDKFNKYKIIPSVQPIHAIEDMEW
                     APLRLGNRIKYAYSYKNLYLQNNILAIGTDFPVSHLNPYNNFCAAVFRKNISGYPIDG
                     FLAEQALSKTEALKSMTIGGAYANFSENTIGSIEIGKKADFVIINKDIMNVTTPQDLS
                     NTYAIATFIDGIPVFILKGNKLF"
     gene            complement(24672..25220)
                     /locus_tag="PKI65_04225"
     CDS             complement(24672..25220)
                     /locus_tag="PKI65_04225"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOB77681.1"
                     /translation="MENLSKELIQKLSKYKWSIIVAHDLNLCIGKDNSMPWHISSDLK
                     RFKQITIGHPIIMGRNTYISLPTKPLPGRLNIVLSRSLKLQKDNLISYSSWQDLSKNL
                     NDNEEYFIIGGSTVFQQALPFVSKLYLTIIHDIFPCDTFFPDYNSLIKTWNKIEHSVD
                     IKENNLVFHYETWQKSILSELK"
     gene            complement(25301..26095)
                     /locus_tag="PKI65_04230"
     CDS             complement(25301..26095)
                     /locus_tag="PKI65_04230"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOB77682.1"
                     /translation="MRQYLDLLQYILDNGIQKSDRTGVGTLSVFGYQMRFDLQKGFPL
                     VTTKKLHLRSIIYELLWFIRGDTNIKYLHDNKVTIWDEWADETGDLGPIYGKQWRSWE
                     GKNNRVVDQLEWVINEIKKNPNSRRLIVCAWNVVEIPEMKLPPCHVLFQFYVNNDRLS
                     CQLYQRSADVFLGVPFNIASYSLLTHMIAQVTGLEVGEFIHTFGDVHLYLNHVEQAKL
                     QLTREPFPLPYLKINTEIKNIDDFKFEDIEIVNYLSHPPIKAEVAV"
     gene            complement(26338..26520)
                     /locus_tag="PKI65_04235"
     CDS             complement(26338..26520)
                     /locus_tag="PKI65_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77683.1"
                     /translation="MRRFRGVATKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
     gene            complement(27213..27340)
                     /locus_tag="PKI65_04240"
                     /pseudo
     CDS             complement(27213..27340)
                     /locus_tag="PKI65_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_053432883.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT        10287 a         3461 c         4281 g         9311 t
ORIGIN      
        1 aacatttatt aatttattta ttattgggat gagtggaagt ttgtataata tcttcatgct
       61 ctttgtggtt aaaaaataac ctatttgtag caatctaaaa ttttattacc cacaataaac
      121 gttatagaac ttaataatta tttaaacttt ttttaataat tttgtagaat agttaatgaa
      181 agatttttta tgaattggag cttagttatt aaaagatttg ttttagcatc gatagttttt
      241 ctttttttaa caagtttttt aaatgggcaa aatggcactt atcgtataca tttattagta
      301 atagatttac ccgaagatca aatatttagg ctaataagta ttaaaggcaa tgcattagaa
      361 ccagtcgata gtttctttag tcaaaatggt gctgttgatt ttgtatattc taaaccttta
      421 ccacgtggtc aatactctat tacgtggcaa aataatcaaa aattcattga ttttttattt
      481 aataatgagc attatatcga attgcatact aatcataaag atccaattca aaatttaaaa
      541 gtaataagtt ctgaagagaa taagtatttt gttaaatttt tgaaaatcaa agatgaatta
      601 tattatttaa tagatatggg ggatgaaata tattctaaaa accccaaaga tcctttactt
      661 ggcataatta ttgataaaat agatagtatc aataatgaat tatcaaaatt tattaaaaat
      721 gtaccagaag aatttttagc atataaagta attaaagcta atatacatcc atcattagaa
      781 gaatttaata aaacacatcc aaataatagg tataatgaca ttaaatcttt ttacaaacga
      841 cattggtttg acaatataga taagcaagac tctgcgctag tgaatacacc tgtcatatat
      901 gaggcagtta aattttatct gcaaaattta gtagagccac cagatacgga gcaatataaa
      961 aaagctgtag atttcatttt aagtcaattt tcatggaatg aacaacaata caaatatgta
     1021 cttaatctac ttttaaatac tttttatacc cctcagtatg aatctgtatt tttatatatt
     1081 tatttcaatt atatgcatga tacacaatgt agtgctagtg tttccgatga aaattataga
     1141 aaagctatga tgataactaa acttagtaaa ggtgccgaag ctcccgaact tactggcatc
     1201 actttagatt cggatagttt taggttaagt aatattacta acaaacctgt tttattaata
     1261 ttttgggcac ccgattgtta tcattgtaag tctataatgc cttatattaa taatctttac
     1321 gaaaaatatg gtgatgatat ttattttgtt agtttctcca tagatactaa tgtagatgct
     1381 attaaaaagg ccacaaaaga agatagtatt aaattcactg taattactga tttacaagga
     1441 tataatggag agaattgtct cagatggtat atttggggaa ctcctacttt ttttatcatt
     1501 gataaaaatg gcaaaattta ttcttcacct tattcatttg aaggtttaga aaatgatata
     1561 aaaactgttt tgctaaatta attcattttc aatagtattt tttaataata attttaattg
     1621 tcaataactc aaaaaaatgt tgataattat attttatttt agcaaaattt tattgtaatt
     1681 ttactttttg aaattaaata ataaaaaaat ttaattaaaa atatctcttt ataaattagg
     1741 taataatata caaaaaaatt aaaaaaaata tgacattatg agtgaaaata agaataagcc
     1801 aaagagttac acttatgaag aagcaataga agcatctaca caatacttta atggtgatca
     1861 gctggctgct accgtttggg taaataaata tgcactcaaa gatagtaaag gtaatattta
     1921 cgagcttaca cctgatgata tgcattggcg tctagcaaag gaactggcac gcatagagag
     1981 taagtatcca aatcctttga gtgctgatat gatttattct ttattaaaaa atttcaaata
     2041 tatagtgcca caaggcggcc ccatggctgg tataggtaat gaccatcaaa tttctagttt
     2101 gtctaattgt tttgttatag gtaatcaggc tgattcttat ggtggcataa tgaaaacaga
     2161 tgaagagcaa gtgcaattaa tgaaaagacg aggaggggtg ggccatgatt tatcccatat
     2221 cagacccaag ggtagtccag tcaaaaatag tgctcttacc tctactggag tagttccttt
     2281 catggagaga tacagcaata gtactcgtga agtagcacaa gatggtagac gtggtgcgct
     2341 catgctcact atttctatca agcacccaga tgctgaggat tttatagatg ccaaagctga
     2401 tctgtcaaaa gtgactgctg ctaatatttc tgttaaaata gatgatgaat ttatgaaatg
     2461 tgtgatcgat gacaagcctt atgtgcagca gtttccaata gattctccca atcctaaagt
     2521 gataaaaact atcgatgctc gcaaactttg gcaaaaaatt gttcataatg catggaaatg
     2581 tgctgagcct ggagttattt tttgggatac tgtgctacgc gaatctatcc ctgatagcta
     2641 tgcagatatg agctttagga ctacatctac aaatccttgc ggtgagatac ctttgtgtcc
     2701 ttatgatagc tgtagactgc ttgctatcaa tctttatagt tatgttataa atccttttag
     2761 tgataattct tattttgatt ttgatttatt caaagaacat gcagcttatg ctcaacggat
     2821 tatggatgat atattagatt tagaattaga aaaaatagat aaaataatag caaaaataga
     2881 tgctgatcct gaacctactg aaattaaaag aactgaaagg gagttgtggg taaaaatcaa
     2941 agaaaaagct atccaaggaa gaagaactgg aattggaata acaggagaag gagacatgtt
     3001 agctgcttta gccttacaat atggtagtga tagtgcgata gattttagtg aagaagtaca
     3061 taaaaccctg gcccttgaag tttataaatc ttccgttaaa cttgctgtag aacgtggacc
     3121 atttccaatt tttgatttca aaaaagaaaa agacaaccct tttttggatc gtttattttc
     3181 aatacatcca aacttagaaa gtgaaatggc taaatatgga cgtcgcaata tttctatgct
     3241 aactatagct cctactggta gtgttagtat attaacgcaa actacttctg gtattgaacc
     3301 agtattttct attttttata aaagacgtag aaaagtaaat cctaatgata aaactgtaaa
     3361 agtatctttt atagatgagt tgggtgatgc atgggaagag tataatgtgt ttcatcctaa
     3421 atttttgact tggctagagg taaaaggata ttctattgat gagataaaaa ataatttttc
     3481 tgatgataaa atagatgagc taataaataa ttctccatat tatcatgcta caagtgcaga
     3541 tgtagattgg gttgctaagg tgaaaatgca aggtaggata caaaaatttg tagatcatag
     3601 catctctgtt actatcaatt tacccgaaaa tgtagaagaa gagttgatag atacacttta
     3661 tcgtacagca tgggagagtg gttgtaaagg tgtaactgta tacagagaag gtagtaggac
     3721 gggagtttta gtgagctcta gtagagatat aaaagaattt ggtgagactc atgctcctaa
     3781 acgtcctaaa aagttagaag ctgatgtgat acgtttcaaa aatgaagagg aggagtggat
     3841 agctgtagtg ggtttatata acgataaacc ttatgaaatt tttacaggta gagccgagca
     3901 tctttatatt ccaaaagata tcactaaagg atatgtgatt agagaaaaag atctaggaat
     3961 ggagcatgcc cgctacgatt ttgtttttta tgataaggac ggctatgaag ttattattcg
     4021 tggactatct cgtacattta atcctgaatt ttggaattat gctaaactaa tctctggcat
     4081 tctacgtcat ggtatgccac ttttaaaagt aatagattta gtagataatt tgaaatttga
     4141 taatgatagt ataaatactt ggaaaaaagg tgttactaga gcattaaaac gatatgtgcc
     4201 agatgggaca aaagttgaag aaaattgccc tcagtgtggc gccgatgatt ctttagaata
     4261 taaagaaggt tgtgtcgtat gctctgaatg tggctattct aaatgtggtt gattttttaa
     4321 tattttatga ttaaaaattt ataatatttc tataatgaaa attatcatac cttcacttct
     4381 tttaccacca atttcttttt ttattgatgc attgaaggct gatgaatggt taatagagat
     4441 aaatgaaact tttacaaagc aaacattcag aaatagatat tatatagcta ctagtcaagg
     4501 tataagagct atgatagtgc ctctatatag acctaatggt agtaataccc ttactaaaga
     4561 tattttacta tcaaaagatt ataactggat agagtaccat cataaaacaa ttataactaa
     4621 ctatagaaat agtgcgtatt tcgattatta tgaagatgaa ttgatcgata ttttttctaa
     4681 atatgacaaa ctatatgaaa taaatacagc tcttttacaa tggactttaa accaattaaa
     4741 ttataaaatc gatataaact atactaatga ttttaaaaat gattattcaa atgattattt
     4801 agatttaaga acaaaataca gaagatacga agcaataaaa ataccagttt atagacaagt
     4861 atttatagat aaattagatt ttataccaaa tctatctatt ttcgatttat tatgtaattt
     4921 aggtcccgaa tcaataattt atttaaaaaa tttaatttta tgaaagcata tgtaactcaa
     4981 tcaatcatcc caatgtacag agatccagat tatcagagcg agcttataaa tgagatatta
     5041 tttggagaat taatagagtt aaatggatct tatggagatt ggactaaagt taaatgtttg
     5101 ttttatgatt atgaaggatg gatatgtagt tatgcttttg acattttaaa tgaagatgat
     5161 gatattagta aatataaaac cattacctct atcactgctc tgatagaaat aaaagatcag
     5221 ataatgtgtg taagtaaagg agctagaatc tatgatttta tgaaaataaa tattgtagac
     5281 ggtaaattta gagaaaataa tactaagatt tctgttggaa atataatcga agatgctcaa
     5341 agtctattat tctcaccata tcgttgggga ggcaaaacac cttttggtgt agattgttct
     5401 ggatttgtgc aattagtttt ttatgtaaat aatatcattt tgcctcgtga tgcaaaagaa
     5461 caaagcaaaa taggtgaagc tcgtatcttt gcacatgatg cttatcctgg agatttagct
     5521 ttctttggaa atattgttta tgacgaagaa aatcaaagag aaatcataaa tatcaatcat
     5581 gtgggaatag ttctgaataa ctctactatc attcatgcat ttaaaagtgt acatattgat
     5641 atgtttgacc actacgggat atatagcgaa aatctaaaaa aatataccca tcaattgatg
     5701 tttacaaaaa aggtattaga ataactaaca atatataatt ttggaactct tagtataatg
     5761 ttggtaaata taaaaatttt aaaaatagct cgcgtttaat tatttattaa taaataataa
     5821 ttatcaatgt gagtggctaa gtttttgaac taaatacaac attgagattt taattttgtg
     5881 taatttagaa tttaaaatta attattatgg aaataaataa taaatattac cactcagttt
     5941 ggggtgattt taaaaatcat agtatcaatt gcatagatca gaggaaatta ccttttattt
     6001 ttgaaatatt ggaattaaaa acaatggatg atctttcgaa tgcaatatct acgatggcta
     6061 tacgtggagc tcctgctata ggtattgcgg cagcttatgg tatatggcta tctcatttat
     6121 taagggatgg taataaaaat ttaataaaag aagattataa aaaattgata aacttgcgtc
     6181 ctacggcagt aaatctaaaa agaggcgctg atttcgttta ttctaatatt aaagatcata
     6241 ttacaaatga aagggtatat aaatgtgcag atcaatttgc caaacaagaa ataaatgctt
     6301 gttaccaaat aggggtacat ggagtagaat tgataaaaaa tatatacaaa actactaata
     6361 aacaagttaa tatattgact cattgtaacg cagggtggct agcttgtgga gattatggta
     6421 ctgctctagc tccattattc gaagctcaaa aacaaggaat acctatacat gtgtgggtag
     6481 atgagacccg cccattaaat caaggctcac gacttacagc atgggaactg gataatgcta
     6541 atattccttt tactataata gctgataata ctggtgctct tttgatgatg aataataagg
     6601 tagaaattgt aatagtaggc gctgatagga ttgcaaaaaa tggagatgta gctaataaaa
     6661 taggaacgta tctaaaagct ctcgctgcta aacaacataa tataccattt atagtggcag
     6721 cacctagcag tacttttgat tttaatatta cagatgggca acaaatacca atagaggaga
     6781 gaagaggtaa agaaatgcaa tctattcaag ttttttatga caataaatta atctctgcgg
     6841 agctttttcc cgttcattat acatctgcta attatgcttt tgacatcact ccacatgatt
     6901 ttgtaagtaa ttatatcact gaaaatggta tttttgactt tattgaccag gtgaacaaaa
     6961 aaatataatt aggtagaaaa tgtttttttt atttttgtaa aaattgatta attatgaata
     7021 aatataaaaa gcatgcaaaa gaactggctg atttcatgcg acgtttatat caacaaaaac
     7081 ttactactac actaggtggt aatataagta tgaaagtcga tgatggaatt ctaataactc
     7141 catctcaaat agacaaatat tcgctaaagg cttatgatat aatacacttg agtatggata
     7201 aaaaggatat caaaagttta aataatgcta gcatagagac acgtatgcat ctagcaattt
     7261 ataaagtcag atcagatgta ggagccatca tacatgcaca tcctttttgg tgtacggtgg
     7321 catcgatttg gaggctaaaa gtagatacaa acctaacgga tgaagcatat tataatataa
     7381 aaaatattgc ttatgctaaa tatttcccaa tgggaagcaa agaattggct aatgaagttg
     7441 cagataaatc aaaatcttca gatgtagtta tgatggctaa tcacggtata gtttgtatgg
     7501 gcaaatctct tgtaaagact ttcgaaaaaa tagaaattat tgaaaaccta tgccatataa
     7561 gctggctaaa gcaatttaat gataaaatca catgtttaac tgatgaagcc aaaaattata
     7621 taaatgaaaa ttattaaaat ccaacctttt actttatcaa aaaatatacc attaattttt
     7681 agaaatttta tcaatctact ttaaataatg attactatta aattttcata attttataga
     7741 aaaattatat atatttgtaa aaaaaataat tatgaaaaag ttttattttt ttataatttt
     7801 aaatattttt atgtggaata gcatttattc acaaaattat ttaactaaaa ttaaaagaaa
     7861 tttgatagaa tatcaagtaa gtgatagttt aaaaaagatg ttaatcgatt acattatcaa
     7921 tgattttagt aaatatagcg atagcttaat ttatttcctt atagatgcaa atggttataa
     7981 atatttgggg tcttcaagta ccaatactga tacttgtgtt atacatatag gatttattac
     8041 agaaatatta ccatctatag aaaaagaaat aaaaggtttt tttaaactta ataataaatt
     8101 attttttgta ataaatggac ataaagattt tttgtatcct actgatagta tcaaggaagt
     8161 agaatatatg agagtaaaga gtcataaaga taatccttta ttagaatttt tattttatga
     8221 agatgatatg ccattttggc tttttgattg caaagatgga gaattttatc taatagatta
     8281 ttggaaaaat tataatttcc cagaatggaa gtgatattat ttgaattttt tgcaaataaa
     8341 actgatgtag agtggagtca tgctatgaca ggtatagctg gagataaagg attaaatttt
     8401 ataacaactg gacatgataa aggtgtagag ccaggaatga cagctttgct aactggacaa
     8461 ttacattatg gttatactgc aagagaattt aatcatagtc atccccaaaa cacaccctat
     8521 ccttctggta tttcaggttt tactggtgaa actggtgatg ttcaatgggc gggtgaagtt
     8581 tgtaaaatat ttggtaataa tgtaaaattt aatatttata ctcctaaaaa tgggaaatac
     8641 atacaattta gtcccaattc taaaaaatct gattattttt aaatatagta gtttatggca
     8701 aataaaaaaa atttttttta tttttataaa tttttaatta catttatagt attaatttta
     8761 ttaaaaaata tattaatctc ccaagattta aaaaaattaa tatatatgaa tgaatatata
     8821 attgttgata caacaatata taatgattta tatgatatca ttttacctga atacaattta
     8881 ttcaataaaa atgaatataa tgcttttttt ataaattatc atattaattc caaaaaattc
     8941 aatttatcaa tgatatatgc accaatttct gataaccttg atacatctat ttatattgga
     9001 tttttttata taaaagataa aatttttatt tttgacaaac cttttaaaga attcattgat
     9061 tgtttggaaa taacaaataa taaaaaggaa tttattaaat ttgagaaaaa aaaatcaaaa
     9121 gatattatta ataaaattat agtagattac cctttatggc ttatggaata taaaaatggc
     9181 aaaatttatt taatagaaaa atatatataa aatattaata tgtaccacta ccactccgac
     9241 catctcggct ccgcctcctt cgtaactgat gcagaaggtg cagtagtgca gcatttgcag
     9301 tatctcccct acggcgaact attcgtctcc cagcgaaaca ctgaaaagtt tgatagtagg
     9361 tacaaattca ccgccaaaga gttagacaat gagaccagct acacatattt cggagcgaga
     9421 tactatgata gcgagctgag tgggtggctc agcgtagatc ctatgagcga taagtatcca
     9481 tcactatcgc catattgcta tagtgcggat aatccggtgg tgctggtgga tccgaatggg
     9541 gaagaactaa atacatatga tggtccacca ccaaaggtta taactaataa taaagaagct
     9601 attaagcatt attataatgg aaatggagaa ccagtaaaaa ttggtgaaaa tattacaaat
     9661 gaattattaa aaactaaaga atttaaagaa tatcataata aaattatatc tggggaagtt
     9721 gaaaataaag gatattttaa tgtaaatatg gaaaaagaaa aaaaatcttt ttttataggt
     9781 catacaaata ttagctataa agttacatct tcagaagata ataagaattg tacagtaact
     9841 tataccatat atactaatga tggtttttgg gatcctgatt atataaaaga aaatatggga
     9901 aacaaaatgg cctttgatat aaatattact ttattatctg caaaggaaat ttttggaata
     9961 gatattggat atattaatac ggaaaaattc aagccagatg gtctgggacc gaatttagaa
    10021 ttaggtggta ccccatatca ttttattgaa acaataatac aatatacatt tgaaaatcca
    10081 ggatattatt aaaatatgat aagtaaaaaa ttattattga caattgtttt cgggacttta
    10141 attattgcat cagcatcact ttatattata gctgatagtg atttatatta ttatggaaaa
    10201 aataatttac caatttataa taaattacca tttgagatga aaccagaata ttggggttat
    10261 agacgaggta tgctaaattt tgttatatta gataaaaatg attttgtaca tattggtaga
    10321 ggtgttggat attgggaata tcctgaaata actatagatt cagtaatttc ctatggatat
    10381 aatgatacat tattgattgc tattgttaaa gattcatgta aacaaaagta ttgtctaagg
    10441 caatacgata ccctatccga agaattaata ccacttcaat attgtaatat tgaaaaatta
    10501 aagcatgact ataatcttaa atggatagaa aatccaaata atccaccata cttaataatg
    10561 tctaaacgtt ttagaagtgc ttttattttt tatatttcat taatattatt tattgtatat
    10621 tttagtaaat atctaaaaga aaagcataaa gagaaaaaca aaaatattca ttaattttac
    10681 ctaacaaaat ttataaatga aaatccacta ccactccgac catctcggct ccgcctcttt
    10741 cgtaactgat ataggtggta atgctgtgca gcatttgcag tatctcccat acggcgagct
    10801 attcgtctcc cagcgaaaat ctaaagagtt tgatagtagg tataaattca ccgccaagga
    10861 gctagacaac gagaccagct acacatactt cggagcacgc tactatgata gcgagctgag
    10921 tgggtggctg agtgtagatc cgatgagcga taaatatcca tcgctatcgc catattgcta
    10981 tactgcagat aatcctgtgg tgctggtgga tcctaatttg atggaactaa atacatatga
    11041 tgatccacca ccaaaggtta taactaataa taaagaagct attaagcatt attataatgg
    11101 aaatggagaa ccagtaaaaa ttggtgaaaa tattacaaat gaattattaa atactaaaga
    11161 atttaaagaa taccataata aaattatatc tggtaatgct agtactcctg gtaattttgg
    11221 aataaatatg gaaaataaaa aggatgcctt ttttattggc aatacaagag tagattatga
    11281 tattacgact tcaaaagata ataaaacttg tacagttact tatacaatat atactaatga
    11341 tggtttttgg gatccaaatt atataaaaga aaatatggga aacaaaatgg cttctgatat
    11401 aaatctcact ttattatctg caaaagaaat ttttggaata gatattggat atattaatac
    11461 ggaaaaattc aagccagatg gtctgggacc gaatttagaa ttaggtggta ccccatatca
    11521 ttttattgaa acaataatac aatatacatt tgaaaatcca ggatattatt aaaatatgat
    11581 aagtaaaaaa ttattattga caattgtttt cgggacttta attattgcat cagcatcact
    11641 ttatattata gctgatagtg atttatatta ttatggaaaa aataatttac caatttataa
    11701 taaattacca tttgagatga aaccagagta ttggaataca agacaagggg ggccaggttt
    11761 taatattatg gataaatata attttgtaca tattggtaat tatgttggat atagggaata
    11821 tcctggaata attatagatt cagtaatttc ctatggtttt aatgatacat taatagtagc
    11881 aattattgcg gattcatcta aaaatttata ttgttttatt caaaatgata ctctgataag
    11941 atctataacg ttaattccac taactaattg taatatagaa aaaataaaac atgattataa
    12001 tttgaaatgg atagaaaatc caaataatcc accatattta ataatgtcta aacgttttag
    12061 aagtgctttt attttttata tttcattaat attatttatt gtatatttta gtaaatatct
    12121 aaaagaaaag cataaagaga aaaacaataa tattcattaa ttttacctaa caaaaaatat
    12181 ttttatgatc accgccaagg agctagataa cgagaccagc tacacatact tcggtgcgag
    12241 atactatgat tcggaattaa gcggctggtt atcagttgat cctttatcgg ataaatatcc
    12301 tggactaagt ccgtatattt atagtgcgga taattcggtg gtgttggtgg atccgaatgg
    12361 tgcaagcata aatcctattt atgatattga aactagcgaa tttttaggaa cggatgataa
    12421 aggattacaa ggagaagcga tattaatgaa taaaactgat tttaagcaag gaatgtctca
    12481 tgaagaagca atgtcaaagg gtaagacatt agataatatg agctttgatg aagcactcga
    12541 gtttgctaac aatggaaaat ttaaagattt tattgataac tataataatt taccaaatag
    12601 accagattgg gatggatatt tgacattaaa tgaggcaaat gaatggtata gaaatggtaa
    12661 tggacagcca ttatttgttg atttatctaa aattgattta tctaatttgt attcattagg
    12721 ggataattat attggagaag aaaaatatat aaatttattt ttcgctagca tttcaacaaa
    12781 tgatgcatta gtatatggaa caattaaatt aaaaagatat cccaatgatc aagtaaaagc
    12841 atatgcagat gtttatgact ttaatatgca taattcaaaa aatccgttaa attggcctag
    12901 aaatattgaa gctaaaattg gtaaagctta tgctggagat ggtaatccat ttaatataaa
    12961 tttttatgga agtaaacaat taaaaaagtt accatggata aaataatttt taaattaaaa
    13021 aatgttctag tattaattat tttttattta ataataattt ttaatttaaa ttgtacctct
    13081 gattattcaa agaatcttgg tagtggatat atttatcgtg atgaaggttg tgaagaaaag
    13141 gaaatatttt gtgagcaagc gaatggtggt aaaataccaa aaactgtatt agtctatgat
    13201 tatgatagac attatataat tgcaaaacaa aaaccccaaa aagatgattc aaatatatta
    13261 ttagatgata ctatatatga atataaatat ggctatgata ctttgtatta ttggattata
    13321 ttgaaagatt ttaaaaaagt gataggtcca ttaactgaag atgaattttt aaaagcgata
    13381 attaaatata aagtatctaa aacattaaag ttatgaaata tataacatgt tgtattgttt
    13441 tattattaaa tgtaaatttc aatttgtttt cacaaaatag agaatgtgat agtacttgta
    13501 ctaatgacgc tatttatata aaaacaaatg aatatgaagg agtaattttg ggaccaaaat
    13561 gcttaaaacg tttattaata aaagatggag atacaataga tgcaccaaga tggacaccta
    13621 caatgcaaga tatagaaaat gctgaaaaat taattagaaa atatgtaaca aaaaaatcta
    13681 agcatcattt agtaaatcaa agtgatggat gtccaattat ttatcaaaat tttgacaaat
    13741 atgttcgtca atattacggt atttttaata aaaaaggtca aaaaatatta gaggttaatt
    13801 ttttatggag agattcaaaa tttatagagg aatggaaaat aaaagggata acaattttag
    13861 atggttgtag ttatttttgg catataagtg taaatatgaa aaagaaaaaa tgttttaatt
    13921 atagtgtaaa tggaattgga taaccaaaaa tattttaatt gttttgtaaa taaaattaag
    13981 aaataaaaaa aatgtttgat acccggtaca aattcaccgc caaagagcta gacaacgaga
    14041 ccagctacac atacttcgga gcgagatatt atgatagtga gctgagtggg tggctgaatg
    14101 tagatccgat gagcgataag tatccatcgc tatcgccata ttgctatagt gcgaataatc
    14161 cggtggtgct ggtagatccg aatgggatgg actggtatga ttttacagat gaaaatggta
    14221 attattctca actttggaga gaaggtaatg cagccactat tgtagttaat ggtgatacct
    14281 atcaaaatat aggtactact aatacgatta gaataaataa aaatgttgaa ataacatata
    14341 cacaaaatga agcaacttca atgacattta ttggaataga aagtgataat tgggaatcac
    14401 aaataacaaa tggcacaaat tgttatgagg ctagttgtca aatgttaaat aacgaaggag
    14461 ttcaaacagc tggtagagct aatgaggttt tagtaacggg attaggtgaa aatggcagag
    14521 caggaaatcc gacagctaat gctcaaaatg gtttcaaaat gatagataat gcattagaac
    14581 atggagatcc aattattgta ggcgtagatt accaaggtgg atcaccaaat tatgataaaa
    14641 tgacagatca ttttattgtg atttcaagta aaactgaaac attagataaa ggaaaagtaa
    14701 cttctaccac atataattat ttcgacccaa gaactaagca taaagattgg gggacatctc
    14761 ctacaaataa attatctata cagaataaca aaatggttgg tactttcaac catgaatatt
    14821 ataattttaa ttatacagtt acaacaatta gaccaaatag ataaaaattt taaaaaagaa
    14881 aaatatgaaa aaaatattag tttattttat aataggtttt tccttactca gttgtaaagg
    14941 acaaaatgaa gaatttaatg attatatatc aaattttcag gaatttaaat tacctttttt
    15001 aattgataga agaaatacac aatacagatt atccacgaat gaagattatg aaatacctgc
    15061 caattatatt aaaaaatata tatgtaaaga ttctacacat tgtgattatg atcaatcaaa
    15121 atatagatat acttatggtt ttaaattcaa atcaaaatat acttttgtat ttttactaaa
    15181 gttaacatat gggggtaaaa catgttatga tttcgattta atagaaacat tgttaatagt
    15241 atattcaaaa gatggtaaaa taatagatgc gaaatccgta gctaaagata atgattgttg
    15301 gcaaagtagt atttgggcaa ctgaagatag cattagagta caacaaataa aagtaacaga
    15361 gctagatgat tttgatgaga taaataaaga acttgactgc gaaatagaaa caaaaatata
    15421 tcagataggt gatgatgggt atataaattt aattaaaaca gaaccaatta aaagaggggt
    15481 acttattttt gataaaaaca ttgatgattt tatattaaag caataaaaat atatcttacc
    15541 taacttcgct tttgccagct attagttatc cacctccttc ccgcacgcat tggcaaatga
    15601 tttgcaataa aacaagcaag cagggttggg tagggctggc cagcgtagtt tttttaatta
    15661 ccccgtcagg caaagcctga caccccttcg gagaaggggt gtcacatcgt attcccctct
    15721 tgtagagggg atgtctcaca gagacagggg tgttaaaact agtggagatc tttcttttgt
    15781 tagtttttct agaagaatag gatgatgcta tttgtgataa aatagaatat aactatctat
    15841 acgaaagcga tagcatatat tactgcaaaa tttttgttaa taataaaaaa aatgttacaa
    15901 gtcatttatt aaaatataaa ttgtaataat tcatgagaat ctaataacga aaaattaaga
    15961 tatttaagcc ataatatttg agaatatgag aaattaagta gtggaggtag gaaacagtgg
    16021 tgacatgtag atgagaaaat ttgaatttat gagttgtcaa ttaaataaat tgcataattg
    16081 ttaaatttat tatttttgca aatctatttt aaataattta tatgagacaa agtaatttac
    16141 aatattttgt aagtcaatac acagaaccgc aaaaagtgca agctgaaggt atttatccat
    16201 atttcagaat attaaagagt ggacaatatc ctttggtaga atatgatggt aggcaaatat
    16261 taatgtttgg ttctaatagt tatttaggtt taactaatca tccacgaata aaagaagctg
    16321 ctaaaaaagc tattgataaa tatggtacag gtacagcagg gtcacgtttt ttgaatggta
    16381 ctatagattt acatttagaa ttagaggaga aattagctga ttttgttaat aaagaatccg
    16441 ccttagtatt tagtactggg tttcaaacaa atttaggagt tatatcttct attacacgca
    16501 gaaaagatta tttgattata gatgaatcat ctcatgcatc tatttatgag ggttctagat
    16561 tgagtttttc taaagtgctt aaatttaagc ataataatat gcaatctctt gaggaaatcc
    16621 ttagtagaat tgataatgat atttcagata caataaagtt aatcatcgta gatggtgttt
    16681 ttagtatgga aggtgatata gcagatctgc caaatataaa taaaatagct cataagcata
    16741 atgctagtat tatggtagat gatgcacatg ggctaggtgt aatgggtgaa aagggtaggg
    16801 gcacagttca tcattttggt atggatgatg atgtggagct gataatgggt acttttagca
    16861 aagccttatc tacagttggt ggattcatag caggtagcaa ggaagctatt aattatctga
    16921 aacataatgc tcgaactatt attttcagtg ctagtttacc cccagcatct actgctgcag
    16981 taattgaaac tttgaatata ataaatgaaa tgcccgaaat gattgaaaaa ttatgggaca
    17041 atactcatta tgctcagaga ttgcttagag aagcaggttt cgatataggt aatagtcaga
    17101 ctcctatcat tcctatatac ataagagatg ataatttgac atttctatat actatgaaat
    17161 tattcgatgc gggagttttt gtaaatccag taattccgcc tgctgtgaaa gaaggtcata
    17221 gtttgattag attttctatg atggctaccc atcaaaatga acatatagaa aaaggaatag
    17281 atatttgtgc taaagttgca aaagatttag gaattattta agctcttata agtacaaaat
    17341 taaaaatttt aaataaatat ttttttaaca atgataaata ttatttcagt aactacaaag
    17401 aaacaattac gagcatttat agattttcct cacgatttat acgaaggcga tccatactat
    17461 gtccctgaac tatatatttc acagagagat ctattaaatc ccaataaaca tccttttcac
    17521 aaacatgcaa aggtgcatta tttcttggct ttaaatgaca ataaagtagt tggacgtctt
    17581 tctgcacata ttaattattc acataatgaa tattataaaa acaaagaagg tttttttggt
    17641 ttttttgatt gcataaatga tagagaagta gcgtacaatt tatttaaaac agcagaaaat
    17701 tggttaaaag atgaaggtat ggatactata gtaggaccag ctaatttcag tactaatgag
    17761 tctgtaggtc ttttgataga tggctacgat agatcaccag tagtaatgat gccatataat
    17821 tatccgtatt atcaagaact aatagaacat tatggttttc agaaaaaaat tgatttattt
    17881 gcttattggc tcgaggcaga acccatatct aataaaatat tagatatagg taaaaaaaca
    17941 gaagagagat taaaaaataa tggatttact attagaaata taagatttag aacacatttc
    18001 aaagaagaag tagaaaaagt ttatgaaatt tataataagg catggagcaa aaatgatgga
    18061 ttcgtgccaa tgactagaga agagtttttt tattcagcta aagatctagc tatgatagct
    18121 aaaaaagatt tttcttttat cgccacgtat aaagataagc ctgtcggatt tgtagttact
    18181 atacctgatg taaatcaagc ttttaagcat ttaaaacgtg gtagactttt accatttggt
    18241 tttattaaat tattatatta ttttaataaa atagatactt atagagtgat tttatttggt
    18301 attatagaag agtatcgcaa aaaaggtata gacgtaatat tttatttaaa atcattagaa
    18361 actgctaaga aaagaaatat ttttcaaggt gaagcctcgt ggatattaga aaataatata
    18421 caaatgaata atatattaga aagcatcgga gctaaaaaat ataaaactta tagaattttt
    18481 caaaaaccta tattatgaat gaaaaagttt tagtaactgg tggttctgga tttataggta
    18541 gtaggttaat taaaaaatta gtagatttag attatcaagt aactgcgata ttacgaaaaa
    18601 caagtaaaag agagcattta cctttagatg atattgatgt agtagagata gatttgttag
    18661 attatcatgg tatattagat tatttcactt tacatagatt cgattacgtt attcatacag
    18721 ctggtattac aaaagcttta aaaaaagaag actactatat acataatact aaaattacta
    18781 aacacctgat agatgcttta ttaaagataa gctggaaacc acagaaattt atttttatta
    18841 gcagtcaagc atcccatggc acttataaag ctctacaaag acctatcaaa attacagata
    18901 ttcaaaatcc attaactact tatggtaagt ctaaactttt ggctgagaga tatatcgaag
    18961 agcattatca taatccattt gtaattataa aaccaacaag tgtctatgga ccgagagata
    19021 aagatttttt aattgttatg aagctcatat caaaaggttt tgagggctat ataggtactg
    19081 aacctcaaaa actttcattt atatatgtgg atgatttagt aaatgcaata atctctacca
    19141 tagctctgcc ttatactggt gaatttttac tctcagatgg caaaaaatat atagacgaag
    19201 atttgggcag atatattaaa aaagctttag gtaaaaaaac tattaaaata cgagtaccta
    19261 tagccatagc ttatatcgct gctttttttt ctgatttgtt ggcacggatc ataggtaaac
    19321 ctactgcttt taaccttgaa aaaatgaaag agctaaaagc taaaaattgg ctatgtgatc
    19381 ctacaccttt ttttgaaact tttcatttca taccttgtta tgatttacaa aaaggaataa
    19441 aagaaactgt agagtggtac aaagaaaatg gttggttata atgaaaaaat attattaatt
    19501 tatagtttta aatttattaa aaataattca gaattatggt ttaatgttta tttttataaa
    19561 ttaaattaat aataagtaaa gtagtaatag ccattaaaat acctaaaata agaaataaat
    19621 aaaaattgtt atttttgaaa acagtagcct gatttacatt gtcgagctct tcatttgata
    19681 taggaggcga tatttctcta atagtatcat taactaataa aagatctgta tatttttttt
    19741 ggtattcata agctttttga taattttcga cagatacata taaatctgct aagttttgat
    19801 aaatctctag taaataatta tacgctttag cattggtggc tatctcctca gcattgataa
    19861 ggtatgctat agctttatct ataaatccca tttctctaaa aaacaatcca tacattgtca
    19921 atgtgtagta tgtcaatctc agatttttag tttcgaaaca taattgcata gcatcagcta
    19981 aatgactttc aaggctatca aactctgaaa gcatagcaaa atattcagca taataaagat
    20041 gactctgtat cagctccgat aaaaatccat atttcaattt tattttataa gaaatatcta
    20101 aaacttcttt tgctttttta taattttcta agtatgcata agtaatgccg agattatcta
    20161 agatttttac ttttttataa atatttttac cagtataccg aagggattta tttaaaaaat
    20221 ctaaagcata attatattta tctaattgat tgaatgtaag tgctagatta atgtatgctt
    20281 ctgttataag tgtagaatca ttattttcta atgccatatt aagaaagata gtgaaatttt
    20341 ctatagcttt tggataatta ttcataacat aatataacat gccgagatta ttaaagtctg
    20401 ctattaattc tttcttatta tcagtagcta tattaatatt ataactaatc ataaaatatt
    20461 tataagctga atcataattt gccatatttt gataaatgac accaagatta ttagctgtta
    20521 atgataaatt taatgagtct ttagcagtat tagaaaattt gaaagcttga ttagtataat
    20581 acaatgcact atcttgcttt cctttgaata tataatatgc tcctaactct ttagctgttc
    20641 tacctagata aaaagtatct ttagtttgta aactgagatt atatgctgca atggcataat
    20701 cgtaaatttc attgtagtct acatacttaa aagtatcgat tttttgtaag taattttcaa
    20761 ttaaagtttt gtgttgacca tttagattaa tgttaaaagt aataaaaatg agtacgaata
    20821 aatatttagt tgaacgcatt tataattttt ttttgcaaat ataaaaaaaa actgccttta
    20881 atgaattaag gcagcttttt tttatataaa acaagtacaa aaacagactt acttaacagt
    20941 ctcagccaat tgactaccta ctttgaattt aacaactttt ttagcaggaa ttataatttt
    21001 ttcgccagtt cttgggttta caccattacg agctttgcga ttagctactt caaaagtgcc
    21061 aaatcctact aaagttactc tctcatttct tttcattgca tcattgacaa ccttcatgaa
    21121 tgcgtttaat gctgcttctg ttttgttttt tggcaattga gcttcttttg ccattgcatc
    21181 gattaattct ttcttattca taatttttgt tttttgggtt tcttatttgc aaatataaga
    21241 acatattgtt tgcaaaacaa ttgtagatga agattttgaa acaaaattgt aagtaactta
    21301 cttgttttgt agataatttt tgatagatta tataaatttt agttaaattt tattaaaaaa
    21361 caatatcatt aacaataaaa taaatctgaa aattttatat aaatataata ttttttataa
    21421 atatatagaa tgtagtagat aaaaatataa tactaaaata tttggtaaag ttgaaagata
    21481 gtaggagcca tagagtttaa aagaaaatga taagcaaaaa cgaggagacg agaaaaagta
    21541 aaaatgttta aatgctttaa atatagaatg ttgtaagttg tttatatttt tgcaaaaaat
    21601 ataaaagatg aaaattttac aagtatgtgg tggatattct atgggaggat tagaacagca
    21661 aactatcaag atagctaagg gactaataaa taaaggacat gagctaatga tatattgctc
    21721 taaagatagt ctgataagct ctaaatgtaa aaccgaaaac atcaattata gcgaaggact
    21781 ttttgatata ggaaacaatg tatttaaaaa ttttaaaaat acctataaaa ttttaaaaaa
    21841 gtttaaacca gatatcattc atgtacaaag atcacacgat ttatttccaa tagttttagt
    21901 tttatatatt tataaaatta atactccatt agtttttact cgtagaatgg aaagtagtat
    21961 aaataaaaaa actttttttc ataaattaat atataaaaga ataaataaag catatgctat
    22021 ttcttcattt attaaagaca atttgatagc tactacacca ataaaacctg ataaagtaga
    22081 agttttaaat aatggtgtcg atttaaaaat tttcaatcca ttaaattatg ataaaaaaac
    22141 ttgtagaaaa aaatatgata tgcctcttga tgccactata ataggtatgg taggcaggat
    22201 ctcgcctatg aaaggtcata aagaatttgt aagagcagcc tctgaattgt ttaacaatag
    22261 aaatgataaa ttgttttttg caataatagg tggtgctagt gtgggtgaag agtcgtttgc
    22321 tcaagaaatt tatcaacttg ctaaaacact cttgccgagt agtaatttta gattttatga
    22381 attcacggat tcagtagctg aggctcttgc tacaatggat atttttattt ttccttctta
    22441 tcgcgaatca tttggtaatg ctttattaga agctatggct atgcaattgc cgatagtaac
    22501 tacatatgct ggaggtgtaa aagatataat aatatgcggt gaaaacgctt tatgcataga
    22561 accaaaaaat catttgcaac tagttaatgc tgtgaatcaa ttattaaata acgaagaatt
    22621 aaaaattaaa atttcaataa aagcacgtaa aaccgcagaa aaacataatt ttgaaaatta
    22681 tattaataaa ttagaaaatg attatttaga tttaattaag aagttaaatt tttaagattg
    22741 ctagttaata aaatatttaa acataaatta agcaacaaca atcaaaaaag tttattaccc
    22801 ttcaatataa aaacaggtat gccatcaata aaagtagcta tagcataagt attactaagg
    22861 tcctgtggag tggtaacatt cataatatct ttatttataa taacaaaatc tgctttttta
    22921 ccaatttcta tactaccgat tgtattttct gaaaaattag cataagctcc gcctatagtc
    22981 atgcttttga gtgcttcggt tttgctaagt gcttgctcag ctaaaaaacc atcaattgga
    23041 tatccagata tattttttct aaagactgct gcacagaaat tgttatatgg attcaaatgt
    23101 gaaactggaa aatcagtgcc tatagctaga atattatttt gtaaatatag atttttgtat
    23161 gaataagcat atttaattct attgcctagc ctaagtggcg cccattccat atcttctatg
    23221 gcatggatag gctgcacaga tggtattatt ttatacttat taaatttatc taaatccata
    23281 ggatttacta attgagaatg ttctatacgc cagcgtaaat cattttcagg cgttaaatat
    23341 ttactatacc aatctaatat tgttcctaca gcagcatcac ctatagcatg tgtagctatc
    23401 tgatatttat atttgataca aagctcagca atataattaa aattgtattc atcaaataat
    23461 aataaacctc ggttattatg gtcatctgaa taaggctcgt ataaatatgc gctacgacta
    23521 cctaaggcac catcagcata taattttaca gagtttacaa taattttatt gtttttataa
    23581 actccatttt ttaatatgtt ttcgatatta tcatcatttg gagttaacat cccaaaaact
    23641 ctcagcaccc atttgtcctc actttgtaat gaatctaaaa gataaatatc ttcgctatta
    23701 atgccggctt catttatcgc agtgatacca taagacaatg cctgctcttg aagtttaagt
    23761 aaagaagaga tttttgcact cttaggcaaa tgaggtatat gactttctac taattccata
    23821 gccttgtcta tcaatattcc agtcaattct tcatttttta cttctataat gccattttct
    23881 actttagtat ctacatttat atttgctaat ttgagagctg catcatttgc taatgcagca
    23941 tgagcgtcta ctcttttaag atatacaggt atgtctggaa aataaatatt tagtaattta
    24001 ttattgggca tctgcatatt tggaaataaa ttttgatccc agcctctacc ataaataaat
    24061 gtaacatctg ggtgctgttt tttatagatt tgaattcttt ctatcatctc atctacacca
    24121 cgtacaccag ttagatctat cggctccatt tcagtactat atgatattaa atggcaatgt
    24181 gcatctatta tgccaggtat tatggtaaat ccttttagat ttattttttc ttttgaattc
    24241 catctcttta ataatgtatc cgaatcgcct acatctacta ttttatcatc ttttatcact
    24301 atagcttcta ctttttcgaa actatcattt actgtataaa tattaccatc ataaaatatc
    24361 caatctgctt tattcataat tttgcaactt gataatatta gacaaaataa aataaaataa
    24421 atacttctta caaatttcat taaaaaaaat ttttaggatt tattacttta aaaaacaaat
    24481 ttataaaata ttattttaaa gaaccaatta tttaatttga aaaatactca tatcaggtaa
    24541 tctatatgta taaatctata attatttctg attttaaatg taatttattg ctaataccat
    24601 caaaattaat aaaaatcaaa aatatttgaa atagataata gaatttgaat gttaaaaaaa
    24661 aaattgattt tttattttaa ttctgataaa attgattttt gccatgtctc ataatgaaat
    24721 actaaattat tttctttgat atctacagag tgttctattt tattccaagt ttttatcaat
    24781 gagttataat caggaaaaaa agtatcacaa ggaaaaatat cgtgaataat tgtcaaatat
    24841 aatttagaaa caaatggtaa tgcttgttgg aaaacagtag aaccacctat tatgaaatat
    24901 tcctcattat catttagatt ttttgataaa tcttgccatg atgaataaga aatcaaatta
    24961 tccttttgta atttcaaaga tctagacaat acaatattga gtctacctgg aagaggtttt
    25021 gtaggaagcg aaatataagt atttctaccc attatgatag gatgccctat tgtgatctgt
    25081 ttaaatcttt taagatcaga tgagatatgc cagggcatag aattatcttt gccaatacac
    25141 aaattgagat catgagctac tataatagac catttatatt tagataattt ttgaattaat
    25201 tcttttgata aattttccat aaatcgattt ttaaatttaa aatatttaaa tgcactaatt
    25261 tttacatttt cttttaattt ttttcttctt caatgcctat ctatactgct acctcagctt
    25321 taataggagg atgtgataga taattaacga tttcgatatc ttcaaattta aaatcatcaa
    25381 tatttttaat ttctgtatta atttttaaat atggcagggg gaatggctca cgagtaagct
    25441 gtaatttagc ctgctctaca tgattcaaat ataaatgaac atcgccaaaa gtatgaataa
    25501 attcacctac ttccaatcct gtaacctgag ctatcatatg tgtaagcaaa gaataagatg
    25561 ctatattaaa aggtactcct aaaaaaacat cagcactacg ttgatacagt tgacaagata
    25621 aacgatcatt atttacatag aattgaaaca aaacatggca cggaggtaat ttcatttctg
    25681 gtatttcgac aacattccat gcacatacta tcaagcgtct gctattagga ttttttttga
    25741 tctcatttat cacccattct agctgatcaa ccactctatt atttttacct tcccaactac
    25801 gccattgctt gccatagatg gggcctagat cgccagtctc atcagcccat tcatcccata
    25861 tagttacttt attatcatgt aaatatttaa tattagtatc accacgaata aaccacaaaa
    25921 gctcataaat aatacttcgc aaatgaagtt ttttagtagt aactaatggg aatccttttt
    25981 gcaaatcaaa acgcatctga tatccaaata cacttagagt accgacacca gtacgatcac
    26041 ttttttggat gccattatct aagatatatt gtaataaatc taaatattga cgcataaaat
    26101 atttttaata tacatgcaaa tataatttaa attttgaaat atattaataa tatatttttt
    26161 taaaagattg tgctatttga taattattta atgatttatg aaaacaaatt aaataataat
    26221 gtaatcatat ccaatgttat tgacttaaac ctactaaaaa aataatatct atattctatc
    26281 tagaaaaagg gaatcgaaag ggatctagaa acaaatcatt gttttacttg aagtagttta
    26341 tctatatttc gtatttacta gccgccaata ctcatcccat gcccatctat ccgaggatgc
    26401 aaggtttaaa agccgaccaa tgtctgattc tatctcactt gtcaagtatt tatgtgtgat
    26461 tacaaaccac atcaaatagc tctgtaagta tttggtagcc actcctctaa atcgtcgcaa
    26521 ccaaaccttg aaatcattta tgtgcttatc gactacctgt acactgtata caccgcgtct
    26581 aattatttaa ttatgctata aactacttca agtaaaacaa aggatatttt tatgtagctc
    26641 cgttcacaaa tagcgtttac gaagcgcaat ttttacaaag ctaaatttcc tatccatttt
    26701 tatttgaata aagactaaaa aaaagttcta taacgttttg tataggtaaa aattaaaaaa
    26761 gagtgatttt ataaaaaatt tgttaatatt ggtttatttg ttataggata atcaaatatt
    26821 aaccacaaag ttctcaaaga atgcacaaag aacgcagaga aggatggaat taaaaaagct
    26881 acaaatggca ctttgtggac tttgtgtaat aacttggtgc cctttgtggt aaaaaaataa
    26941 cctaaataat taaaaaatag ttattttttt gtaataatct aaaattttat tacacaataa
    27001 aagttataga acctaaaaaa attattgtaa ttgtgaaagg aatcgaaagg ggtctagaaa
    27061 caaatcattg ttttacttga agtagtttat ctatatttcg tattcaccag ccgccaatac
    27121 tcatcccacg cccatctgtc cgaggatgcg aggtttaaaa gccgaccaat gtctgattct
    27181 atctcacttg tcaagtattt atgtgtgatt acaaaccaca tcagatagct ctgtaagtat
    27241 ttggtagcca ctcctctaaa tcgtcgcaac caaaccttga aatcgtttac atgcttatcg
    27301 actacctgta cactgtaaac accacgtcta atctgtgttt
//
LOCUS       DAOPRN010000024        26411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1478_length_26411_cov_95.371984, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000024 DAOPRN010000000
VERSION     DAOPRN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26411
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1478_length_26411_cov_95.371984"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PKI65_04245"
     CDS             complement(<1..133)
                     /locus_tag="PKI65_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HOB77684.1"
                     /translation="MQITGTHFNYYYLCKRKLWLFANGINMEHTSDLVYDGKLIHETS
                     "
     gene            complement(146..2431)
                     /gene="cas3"
                     /locus_tag="PKI65_04250"
     CDS             complement(146..2431)
                     /gene="cas3"
                     /locus_tag="PKI65_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOB77685.1"
                     /translation="MRKFCPEILAKSEPPVSLKQHIEECLTVYESLKNAFERLPVSNS
                     EHFWEIVRFGIIFHDLGKSHVEFQKMLNGEYAEWYHQRHELFSVPFIDMLNLENDDKW
                     LLKMIIAGHHKDFKSLYDLILHNYKSEEDFFSFGQDGKLDWDQEVEKLNIPQIQSLLE
                     KFGVSIKLNKFILPVELIKKYNRNPINSSNSHFKELVLAAGALKQCDHSASAGIFKVN
                     MLEDKHFDFLYQKQWIPYYHQKKASETIGNIVLTAPTGSGKTEASLMWLHKQMKEFGQ
                     GRTFYILPFTASINAMYERLNDKMQGNNEIVGLIHGKLSEYIENKFGDEIYSSKNEKL
                     KKELKESYRALVPPLKVATPFQLLKSIFGLKGFEKGIFEMSGGYFIFDEIHAYDPEVI
                     AQIKVLLEFAINHLNVNVCLMTATLPTFLKNKLCEIMQPHTEIYADDNLYKKYIRHKI
                     RVAKGLLSENIPIIQQRLEEGDKVLVVCNTVKQAQSIYQLLDSPNKVLLHGAFNGRDR
                     NKKEDKLQSENVKLLVGTQAIEVSLDIDYDVLFTEPAPLDALLQRFGRVNRHRINDEF
                     RPPCDCVVYSERNDRDKYIYRNEEVIRRTMHVLQEIELKNEGIVFEKDLQHYLDFVYP
                     AWSKNEEEDFDRVYNSLNDFVKNNLVPFIYDPHSEEEFENQFDGIKVVPSILKNDYIN
                     LLNSNQFIKAESLKVSVSKRRFSSLIQNEEIQREVAAFQVLNKEQIMEKTFYTINKKY
                     DDELGLQIDDEYISVESNIWD"
     gene            complement(2428..3087)
                     /gene="cas5b"
                     /locus_tag="PKI65_04255"
     CDS             complement(2428..3087)
                     /gene="cas5b"
                     /locus_tag="PKI65_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HOB77686.1"
                     /translation="MRAFRIKINSWTSSFRYPNIISGFQPTLEVPPISTVMGLINSAL
                     GKYLENCEIELAYYFDFKIKTIDLETIYQIKANDKNFPENTVKPNVIQREFLYDCRLF
                     LYLANEKLLSAFYNPFFPLLLGRSGDLASIESIKEVELQEINNARIGGQVIPFNGNYL
                     PGQIQALPKYFSNTIPRKNIGTEPYSVISYYNPIDTQLTGYRASLEEFDSDIFLHKFS
                     I"
     gene            complement(3087..>3680)
                     /locus_tag="PKI65_04260"
     CDS             complement(3087..>3680)
                     /locus_tag="PKI65_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HOB77687.1"
                     /translation="CAIMKGMFSLDLSMIGTFSNYNRSGFKNLSSALLEEAIEKGLEQ
                     IDDPFILDKNGTPKKLIRLPKDVRKQRAIDTIQALKSISGGAMQTSNMGDVTPKFLIL
                     ATTNTGNHPFSHIAGSRGQWDEEFVFNYEGLIEVLKDYQETFEGTIFIGRRFGFMDEI
                     DSKLKELENMEGIPPVKILTINDAIDQYCEQLANQME"
     assembly_gap    3681..3780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3781..4384)
                     /gene="cas7i"
                     /locus_tag="PKI65_04265"
     CDS             complement(<3781..4384)
                     /gene="cas7i"
                     /locus_tag="PKI65_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HOB77688.1"
                     /translation="MKQRTKKNNLLKNIDMKNLKTQGFILIDVDVVALNNAGKNTQSN
                     FDNAVATKTIRKNGRNYVYVSGQAWRYWWRDTLQKIHGWNLSPVIRDNKIAFTEANPL
                     KYPDDDVFGYMRAAKDAELDENGEPIKDKRGNAKMTNVTVTRVSPLKNSAIISATTVY
                     PEEHWSTLSRQEGDAVPYGKQEYCAIMKGMFSLDLSMIGTF"
     gene            complement(4368..5801)
                     /locus_tag="PKI65_04270"
     CDS             complement(4368..5801)
                     /locus_tag="PKI65_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77689.1"
                     /translation="MKETYKTIDYKWLTKPTGDPFADAGGFAIEYLLEKSPEKDILEL
                     IEYITKIYVEKWDGKINAFFLNSKITQPAFDNKRKIEETLKYFESLINESEPYEIGYC
                     RITGQKTKLFFAGRDNSIMTGSGTFINFHHFFQKGISLSKEALIRMYFIPFVCQQLEG
                     KICLIQSSNEKLSKLFVKRTIDQNLKDIATGTSTRIAKSNFNKPANAIFDFVDYSLNY
                     LNELKKEEDIMPSLTLYHFTNFGASPEIKIYQLPSQVFLFYRTCMQPKFKTDWQNFVR
                     SHYFDNKKYRSAKYNPQAGTFELIKSKETETIDFEEYKQWSNRIYNKLISNKSIIPEI
                     LRWSRKHPFKFEIVSIYQQNIMSMKKETINKIKELAVFLVREENADKIKKRIRALDSI
                     KNAAGLRRFILRDVIAENYNSGNKEAILTLEDYINYLFPDGSHWSEIRDLLLIAIYQE
                     MHERNLISEELKVDFESEEIEEIEAEN"
     gene            complement(5813..6607)
                     /gene="cas6"
                     /locus_tag="PKI65_04275"
     CDS             complement(5813..6607)
                     /gene="cas6"
                     /locus_tag="PKI65_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOB77690.1"
                     /translation="MRFKLIINIDKSKGRDKLPINYQYECSAVIYKILSNSNEKFSQW
                     LHDNGYNADKKLFKLFTFSRLKIPQYRIINEYIRILSDHIEWQISFIPEISTREFIQG
                     IFREQEFELGNRNARIKCRIQNIEMLPPPEFKETMTLKTLSPICLTLKRDNGSDEYIS
                     PTHPMAMPIIKQNLLDKYKAFQGKDFPDHDFAFDFKVINEPRSSLITIKADTPQESKI
                     RGFSCQFQLTAPIELMKIAYDGGVGSKNSLGFGMVREWEKINIKKK"
     gene            6984..10220
                     /locus_tag="PKI65_04280"
     CDS             6984..10220
                     /locus_tag="PKI65_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOB77691.1"
                     /translation="MNEITRHPILDVPKSEAVEFLFNGQRVIGRKGYTIASALHLAGF
                     PIHSHSIDGRPRSLECGIGKCSACEMLVDGVVRRICITKVDGVKEVIEIPQDYRPEPK
                     KLSLDLPNKLYHTEVVIVGAGPAGLAVREILNDFGVPNIVIDSNESIGGQFLMQTHQF
                     FFFEKQRKYGGKRGFEIAKMLAGDDHSDILLDSVVWDIFDNKILAVKNIKTEDIFFVK
                     CEYLVVGAGAVPFIPVFENDDLPGVYTAAVVQKLMNIEHTLLGKKVLTVGAGNIGYLT
                     SYQLTQAGANVVAIIEAQQNEGGFPVQANRVRRLGIPILTGYTLVKAIPNDDYTAIIG
                     AVIAKADEQFKPISGTEKLIENIELINICTGLIPDDQLLVLGKEIFGTKCHGVGDAIR
                     IGEGTSAVLRGKQAAYEILSDMDIRYDYDDYLAVSKEYIDSQQHPFKVLDSPAQPDEQ
                     RKSKPFVIIDCLYAFACNPCVFACPQNAISKSSTSNVPVVDYDKCTGCFDCVYQCPGL
                     AIIGFNINKNKIFIPTEHFLEENSQVFLVDNYANKIGEGVIEKIMIKPNKTNVARVRV
                     EHLFDDNITNIRGLIPKNQYPEPLVLKDYNDTISFDDPYVCHCEDVKYQEIKDFVGDR
                     KFVSVMDIKHNTRLGMGPCRGKRCIRRLRQRLYSDGITLVGDATPRGPLSNQILLGEL
                     YPRTIKDKIIPIGTKKDIEHREVKVLIAGGGMAGSSLFRYFAEAGMSPVLINYEKGSS
                     WRNIAGGRPAFSIPELSDIARHNLEIFQELNEYKNINFKPTVYVNFAHDQETYKSLEA
                     SKGWSDVYEVKPKDFKNEISPYFNPTLDTYLAAFISRECWQANPGKTVDLIRRIGLEN
                     GGTIYEEAILISVNKKNNKYFAIVKMRDGSYIEFQTDHFVNALGGEAEKYANMLDLYP
                     GLYPVRHQAFITRRLPYLGKNGNSLDMLIDRRNFKGFSAVYGQQLYETGQIIGCASPA
                     VDSMESSKDIKLNTREFMSIISEVFCDWIPMLSSVQFQSIWSGYYTEPRMIIDPENGL
                     FVGMRGHGFMLSQYLAKLYVDKFLGRGVPSYFEELKLTGKGLSETAFK"
     gene            10353..11507
                     /locus_tag="PKI65_04285"
     CDS             10353..11507
                     /locus_tag="PKI65_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77692.1"
                     /translation="MSQKILTWHRKIFDNISRQYAEQRLPHAILLSQRMSENAFFLAK
                     SLAQLFQCSNPQLNNEGYLEACDLCSSCKKVKEFTHLDVHYFFPIIKRKNKTCSDDYF
                     QEWIEYLRSYRGIIDLNSWIRFIDSANSQAIISAEDCDKIVNIAQDSSYEGKYKIFII
                     WEMERLFHAAAPKLLKIIEEPPAKTLFVGITDHLEQVLSTIRSRMQIIQIPTPTPEES
                     LEILNDFFDIEKDEAKRLVFTYGNNLPRIVDNIHTDKMPIAISDFFIKWMRLSFQLKA
                     HELLDLAIEFNSLGRDIQKITLSQCLNYLDAIWLINHNIPTQLILNELDEKFVHDFKK
                     FIDDDTIADFACYVDEAIKNIERNADSKIVFFDLSFKIGQTFKQKYMMMR"
     gene            complement(11883..12650)
                     /locus_tag="PKI65_04290"
     CDS             complement(11883..12650)
                     /locus_tag="PKI65_04290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOB77693.1"
                     /translation="MKNCLFKVCHAINYIITRKNPIKIHSPFLYNMYLNAYKTNYDIP
                     NELLDYENKLKHSNEPLRFQSLGANKRFIETTISYWHRKISCSLNKRLFIIGLAQYFK
                     TNKILELGGGFGFTSALLAMSNTDLEVYTIEGVDDLCKQLLKLKNRLKIDNLHVYNLE
                     FDEAIDEFISKNIKFEIIIIDGSHTYEATLNLINKITKIIAPLSIVILDDINYSYSMY
                     SAWKNIKKIDFFEVKIERYNYGLLIKNSNLSQQFLKI"
     gene            complement(12676..12951)
                     /locus_tag="PKI65_04295"
     CDS             complement(12676..12951)
                     /locus_tag="PKI65_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOB77694.1"
                     /translation="MSLDDRLKILRLELDELDTKIFNLLIKRYELCKEIGKLKHSLNI
                     PLYQQERYNEKKNKIITKAKKANLPPDYLEEIYELIHKFCIKVQSME"
     gene            complement(12963..13727)
                     /locus_tag="PKI65_04300"
     CDS             complement(12963..13727)
                     /locus_tag="PKI65_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOB77695.1"
                     /translation="MKFYNLILILLLISLTSCSSYYYPNLLMTNLTENDFRDLKELQV
                     APSQTIKKGDQFNILVYPNNGEALLIYESDIRRINQEVSNIIYYVNEHGFISLPLIGN
                     IFVEGLTLETLKDTLTKLYSSYITDPYIFLGWANKKVYVFSQGSGSGTAVPFSNSYIT
                     LLDAITSTGGIGNEAYSNKIIIARATDTNKEDFAFYKINLQDKENLYLGNIYLRDGDM
                     IYVLPKKKFVDKALAEINPYISLFNTLLIIIQFFIL"
     gene            complement(13881..14873)
                     /locus_tag="PKI65_04305"
     CDS             complement(13881..14873)
                     /locus_tag="PKI65_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOB77696.1"
                     /translation="MRFNSDTEAAKSLVEFYENLKNETAKVIVGQQDVIDKLLIALFT
                     QNHALLIGVPGLAKTLLITTIAQILGLSFNRIQFTPDLMPSDILGTEVLDEHKNFHFI
                     KGPIFANIILADEINRTPPKTQSALLEAMQERSVSIAGNTFTLDRPFFVLATQNPIEQ
                     EGTYPLPEAQLDRFMFSIWLDYPSIDEEIEIVKRTTFVDMPTVNNIIDKDTLLEYQKL
                     VRKINVPDHVYEFAVKITAMTRPVYEDSHPMAKEYVAWGGGPRASQFMILGAKCHALL
                     NGKFAPDNEDVKAVADSVFRHRLVLNYRAEAEGVNTTDIIHEIIKDIEKTKVKF"
     gene            complement(14877..16241)
                     /locus_tag="PKI65_04310"
     CDS             complement(14877..16241)
                     /locus_tag="PKI65_04310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF020872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOB77697.1"
                     /translation="MKKIFLALFIIVASFISLFSQSQPPQVLDKIIAIVGNKIILKSE
                     IEMYVQQMLLSGEIDKSIRTDELSCEILEEMLLQKLLLMQAEIDSIEVSDEQLDNELD
                     RRIRYFTTLFGNIQELEKYYEKTVLELKEELREPLREQLLIMTMNDKLLSNVNITPSE
                     VEKYYKKLPEDSIPIIPAQWELGVIAIKPTLSQEEKNILISKLQDIKKRIEDGADFEV
                     MARLYSEDPATAKNGGATGYFTKGIMEPSFENTAFSLTKPGEISPIIETSYGFHLLQL
                     IDRKGEQVNVRHILIRKQPSNQSIQLANKKADSIYNLLINNEITFEEAAKKYSYLEGE
                     LTQGGLILSSSGGFTLTSNDIDPKYFPILDTMKNSKYSKPFLYLEADGSPTYRILWLK
                     NYIPPHKASLKTDYFLIQLQALEEKKRQALEKWVTDRLKDVYVKIDIEYHGCDFKHPW
                     IKKY"
     gene            complement(16246..18234)
                     /locus_tag="PKI65_04315"
     CDS             complement(16246..18234)
                     /locus_tag="PKI65_04315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOB77698.1"
                     /translation="MKKYIGIVIFSLLLSMTLMGQNEKILLIIENQEVPVSEFLEVYG
                     KNNRNPDFSKASIDEYLDLFINYKLKLMEVQRLRLDTQKTFINEFQRYRDQLTQTYLI
                     DKKTIDNLLQEAYNRMQHDVRASHILVKITPYANPKDTLKAYNKALDIRKKLLSGMDF
                     AKLAVEMSDDNSAREQTATDGRIIPGNGGDLGYFNVFNMIYDFETAAYNLKIGEISTP
                     VRTQVGYHIVKLNDKRPALYRFKVAHILLMYPPDADETTKELIKLRADSIYQNLVRGE
                     NFSELAKKYSDDKGSAINGGELPWLSPFRLVPAFVEPIYDHKPGDIIPPVKTFYGWHI
                     IKLIDKQTPPDFSQAKQELLVKLNRDPRMEIAQKVIIDSLREVYKITYDKKSLTNIAK
                     AINKDSLINNNFKESQIANLDNILVKIDDYSIAVSEFATFIKSNQSLFSNTDDVHVFV
                     NKMFDKFLDKKILEIEKENLELKNPKFAEQINEYKEGILIFNLMDQNIWSKAMKDTAG
                     LKAYYETIKHNYLTEEKAVLAVFKCNNQKIADKVYKSLQKGLKKSNLNPNDILTKINK
                     KDSIVDYDTMVVETNKLADLGIEKVVNTLASKTKDDIYEIYWLKQIIPPQPKSLNEIR
                     GLIISEYQTYLERNWINELRNRYQWQINEDVLRSLYKD"
     gene            complement(18256..19719)
                     /gene="guaB"
                     /locus_tag="PKI65_04320"
     CDS             complement(18256..19719)
                     /gene="guaB"
                     /locus_tag="PKI65_04320"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOB77699.1"
                     /translation="MFKNKIINEGITFDDVLLMPAYSEVLPREVDTTTYLTRDIKINI
                     PLISAAMDTVTEAKMAISMAQEGGIGVIHKNLSIEDQAKEVKKVKRAENGMIIDPITI
                     FAHNTVEDALKLMCENHIGGIPVVDENNKLIGIVTNRDLRFENNYSKPISEVMTKENL
                     ITVSEFTDFTTAEAILQKYKIEKLPVIDNNGTLIGLITYKDIMKVRERPNSCKDELGR
                     LRVAAAVGISSNTLERVEALLSVNADAIVVDTAHGQSAIVLETIKKIKSTFKNIQLIA
                     GNVATTEGALALAEAGADCVKVGIGPGSICTTRVIAGVGVPQLSAIIYAANALKDKGI
                     PIIADGGIRYTGDIVKALAAGANSVMIGSLFAGVEEAPGETIILEGRKYKVYRGMGSI
                     EAMQQGSKDRYFQDMEEDIKKLVPEGIVGRVPYKGNISEVIFQMMGGLRAGMGYCGAK
                     NINDLRKAKFIRITNAGVIESHPHDVTIIREAPNYSR"
     gene            19848..20462
                     /locus_tag="PKI65_04325"
     CDS             19848..20462
                     /locus_tag="PKI65_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOB77700.1"
                     /translation="MLEVILGWDKALLLLINHARSEFLDVILYLVSQQYFWVPLYVIV
                     LILLIKFYKKKAWAYILLFFILLLFTDQSSVFIKNNVKRLRPCHEPEIKNSVILVKNK
                     CGGKYSFVSSHATNCMGFATLAWLLLKRFANKKWKKTLLAIVLFGLYVFTVIYSRVYL
                     GVHYPIDVFMGTILGALLGYLVYLFSERFFPKLFAFSFKNDNDS"
     gene            complement(20424..21863)
                     /locus_tag="PKI65_04330"
     CDS             complement(20424..21863)
                     /locus_tag="PKI65_04330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOB77701.1"
                     /translation="MKPPLNKMHHTTRYLLLDLISSIIGWLFLSFYRKNLMYQGIYDF
                     STLSNMIFNDINFYKGVIIIPFIFIGIFALSDSYREVYRKSRLKVLFNTVLQCLIGVT
                     IIFFIVVLDDYPYYHYKYLYNVYFSYFGVSIALTYILRLSLMTWTKNGIKKKKIVFNT
                     LIIGNNNLAEKIYQSILKNSYDTGQHVVGYIKINGGKDTLHEIVPCLSKLDDIGDIIL
                     KYYIEDIYIVLEPADYDKISNVLSIREIFNVNIYIVSDVKNILVTSSVKIHPFSNIAY
                     IEVPKYLLSTWQSILKRTIDIFISTLALILLSPLLVFIAIKVKKSSPGPIIYKQERIG
                     RYGIPFNIYKFRSMYIDAEANGPALSCKDDDRVTPWGKVMRKYRFDELPQFFNVIKGD
                     MSLVGPRPERQYYIDQIVKIVPYYRLLLKLKPGITSWGQVKYGYAENVDQMVERLYYD
                     LLYLDNISLFSDFKILILTIIIIFKGEGK"
     gene            complement(21878..22738)
                     /locus_tag="PKI65_04335"
     CDS             complement(21878..22738)
                     /locus_tag="PKI65_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77702.1"
                     /translation="MKNLISLIFLIFFVVFFTSCEWLDPDEKLPCYIKIDSVKLNVRP
                     GEGSASHAINTVWVYVDGQRSGVYELPAKFPLLFEGSNEVIIMPGIKLNGISTTRAVY
                     PFYDDYKVNINLSPLEEIELVPVYHYVEGIKFHFIEDFETPGSIFTSHSTSDTFMINT
                     DDATEVFEGNFSGKISLTDDRDTAIITTINSYKLAPGLMHFLELNYKSTENLTVGIMT
                     YTNTGPLTYPYVILKPTDIWKKIYINLSPLASRYGQNSNFIIFFATYKTSTNSEGKIL
                     LDNIKLISSE"
     gene            complement(23001..23993)
                     /locus_tag="PKI65_04340"
     CDS             complement(23001..23993)
                     /locus_tag="PKI65_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOB77703.1"
                     /translation="MRKIGVIGAGHLGKIHINCIKEISGYDLVGFYDVNPLVASDVQQ
                     KYNIPAFENFEELLEKVDVIDIVTPTVSHFEYAKKSLEAKKHVFIEKPIVTTLEEAKE
                     LMKISKNAGVQIQIGHVERFNPAFLATRSYINEPKFIEAHRLSYYNPRGTDVPVVLDL
                     MIHDIDILLTIIDSPIKGIYANGVPVLTQNIDIANARIEFEDGSVANLTASRISMKKM
                     RKMRLFQQDSYISMDFLNKQSEVIKIEDNIKNDTNDNVFSFPIQLNNDNLKYVVVKKI
                     NLPEINAIKTELEKFLESIELGIKPEVTIDDGYKVLDVAYKIINEIQYNLSNLI"
     gene            24536..>26411
                     /locus_tag="PKI65_04345"
     CDS             24536..>26411
                     /locus_tag="PKI65_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77704.1"
                     /translation="MNVKQILSISIMLLWATFAFGQLQGLVEVNPTDPVYPTLSAAID
                     SLNNQGVGDGGVTITVAPGNPQTAPAGGYVITASGDISKQIIIDGNDNIVTAFSPQDE
                     GKINDAIFKIIGGDWITIKNFVMMENPANTITTASSNNMTEFGVALFYATPDDGPQNC
                     SIIGNTITLGGPYQNTFGIYANSRHTATAMTSGADITSLDGAFNNLQILNNTISNVNM
                     GVVLVGSTTGDYMARNIVVDGNNITYGYTGSFSNYYSVSGTVNGILLNSIVNATVINN
                     KLTSDNTVTAGTLRGIYHYVSGTGAALPTSFAIVNNFNNNNIFLKRNAANASIYGIHL
                     DNYNDSVTNYVIADTIRGLGSAATYTSAVYGIYHQGAAKNQYFKKNIFQINTNTSGSV
                     YAINGGNTIKPNGIQMLDSNRVDYLGKTVGGGTVYFYYSNSSSDSTVKKYFTNNVIDS
                     VTLTGAITFYGYYDRDGGASSAAKKYFNNNLIKNITGGTSAAYGFNVTYGKVIDIHNN
                     VIQQLTNGGNINCIYAGGSSTLDMKIYNNKIQNITTTSGIINGIYSAANATGAIANIY
                     NDTITDLNSAGSVYGIQIYSGITTNVYNNLLYNLNSSGTTGTVYGIYAYGGTTNNIYN
                     N"
BASE COUNT         8562 a         4050 c         4000 g         9699 t
ORIGIN      
        1 aagacgtttc gtgaataagt tttccatcat aaaccaaatc ggaagtatgc tccatgttga
       61 tgccattggc aaacagccag agcttacgct tgcatagata atagtagttg aaatgtgtgc
      121 cggtaatttg catagctgat tttgtttaat cccaaatatt agattctacc gatatatatt
      181 catcatcaat ttgcaatccc aattcatcgt catacttttt gttaatcgta taaaaggttt
      241 tctccattat ttgctcttta tttaaaactt gaaatgcagc aacttctctt tgaatctctt
      301 cattttgtat caatgaacta aatcttcgtt tcgaaacaga aactttaagc gattcagctt
      361 tgataaattg gttggagttg agcaaattta tatagtcatt ttttaaaata gaggggacaa
      421 cttttatccc atcaaattga ttttcaaatt cttcttcgct atgaggatca tatataaacg
      481 gaacaagatt gtttttcacg aaatcgttca aactgttgta aactctgtca aaatcttctt
      541 cttcattttt tgaccatgcc ggataaacaa aatcaagata atgctgaaga tccttttcaa
      601 aaacaatacc ttcatttttt aattctattt cttgtaaaac atgcatcgtt cgtcgaatta
      661 cttcttcatt tctgtaaatg tatttatccc tatcgtttcg ttcagaataa acaacacaat
      721 cacaaggtgg acgaaattcg tcatttattc tatgcctgtt aactcggcca aaacgttgta
      781 ataaggcatc aaggggggca ggctcagtga ataatacatc gtaatctata tccaaactga
      841 cttcaatagc ttgtgtgcca actaaaagtt taacattctc cgattgtagt ttatcttctt
      901 ttttatttcg gtctcttcca ttaaatgctc catgtaataa cactttgttt ggagaatcaa
      961 gtaattgata aatgctttga gcttgtttaa ctgtattgca aacaaccaaa accttatctc
     1021 cttcttctaa acgttgttgg ataatcggaa tattttctga aagcaatccc tttgctactc
     1081 taattttatg tcgtatgtat tttttgtata aattatcgtc tgcataaatt tcagtatgag
     1141 gttgcataat ctcacacaat ttatttttta aaaaagtagg gagtgttgca gtcattaaac
     1201 atacgttgac attcaaatga ttgattgcaa attctaataa tactttgatt tgagcaataa
     1261 cttccggatc ataagcatga atttcatcaa aaataaagta accgccactc atttcaaaaa
     1321 ttcctttctc aaatcccttt aatccaaaaa tagatttcag taattgaaaa ggcgtcgcta
     1381 ctttcaaagg aggaactaat gctctataac tttccttgag ttctttttta agtttttcgt
     1441 tttttgaact gtagatttcg tctccaaatt tattctctat atattctgat aattttccat
     1501 gaattaaacc gacaatttca ttattacctt gcattttatc attgagtcgc tcatacatgg
     1561 cattgattga agctgtaaac ggtaaaatgt aaaatgtacg cccttgacca aattctttca
     1621 tctgtttgtg aagccacata agagaagctt ctgtctttcc tgaaccggtt ggggctgtaa
     1681 gtacgatatt accgattgtt tcggaagctt ttttttggtg ataataagga atccattgct
     1741 tctggtataa aaagtcaaaa tgtttatctt ctaacatatt cactttgaat attccggcag
     1801 aagcagaatg atcacattgt tttaaggctc cggctgccaa tactaattct ttaaaatggg
     1861 aattacttga gtttatggga ttgcgattat actttttaat taattcaaca gggagtatga
     1921 atttatttaa tttgattgat accccaaatt tttctaaaag tgattgtatt tgtggaatat
     1981 ttaatttttc cacctcttgg tcccaatcga gttttccatc ctgcccaaaa gaaaaaaaat
     2041 cttcttccga tttataatta tgtaggatta aatcgtacag acttttgaaa tctttatgat
     2101 gcccggcaat aatcattttt aataaccatt tatcatcatt ttctaaattt agcatgtcga
     2161 taaatggtac agaaaacaat tcgtgccttt gatgatacca ttcagcgtat tccccgttaa
     2221 gcattttttg aaattcaaca tgacttttac ctagatcatg aaaaataatg ccaaatcgga
     2281 cgatttccca aaaatgctcc gaattagata cagggagcct ttcgaatgca ttcttcaatg
     2341 attcgtacac agttaaacat tcttcaatat gttgtttcag cgaaaccgga ggttctgatt
     2401 ttgccagaat ctccgggcaa aattttctca tatgctaaat ttgtgaagaa aaatatcgct
     2461 atcgaattct tctaaagaag cacgataacc cgttaattgt gtatcaatcg gattatagta
     2521 agaaataacc gaatatggct ctgtgccaat atttttacga gggatcgtat tactaaaata
     2581 ttttggcaag gcttgaattt gtccgggtaa ataatttccg ttaaaaggaa taacttgtcc
     2641 accaattctt gcattattga tttcttgcag ttcaacctct tttatacttt ctatactagc
     2701 taaatctcca cttcggccta ataaaagcgg aaaaaaagga ttatagaaag ccgagagcaa
     2761 tttctcatta gccagataga gaaacaagcg acaatcataa agaaattccc gttggattac
     2821 atttggttta actgtatttt cagggaaatt tttgtcgttt gcttttattt gatatattgt
     2881 ttctaaatct atagttttta tcttaaaatc aaaataataa gctaactcta tttcacaatt
     2941 ttccaaatat ttacccaatg ccgaatttat taagcccatt actgtgctta tcggtggaac
     3001 ttcaagggta ggctgaaaac ccgaaattat atttgggtat ctaaaacttg aagtccatga
     3061 gtttatttta atacgaaagg ctctcattat tccatttggt ttgccaattg ttcacaatat
     3121 tgatcaattg catcgttaat tgtaagaatt tttactggtg gaataccctc catattttct
     3181 aattctttaa gtttggaatc gatttcatcc ataaaaccaa aacgacgtcc gataaatata
     3241 gttccttcga atgtttcttg ataatctttt aatacttcga ttaatccttc ataattgaat
     3301 acaaactctt catcccattg acccctactt ccagcaatat gactaaaagg atgatttccc
     3361 gtatttgtag tagctaaaat taggaattta ggtgttacat ctcccatatt gctagtttgc
     3421 atagctcctc cagagataga tttaagtgct tgaatagtat cgatagctct ttgtttgcga
     3481 acatcttttg gtaaacgaat taatttttta ggagttccat ttttatcaag aataaaagga
     3541 tcatctattt gttcgagtcc tttttctatt gcttcttcga gtaatgcaga tgacaaattt
     3601 ttaaatccac tacgattata atttgaaaat gttcctatca tggaaagatc cagactgaac
     3661 attcctttca taattgcaca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 aaaatgttcc tatcatggaa agatccagac tgaacattcc tttcataatt gcacagtatt
     3841 cttgttttcc gtatggtacg gcatcccctt cttgacgtga caacgttgac caatgttcct
     3901 cgggatacac ggtcgttgca gaaattattg cagagttttt taggggagaa acccgagtta
     3961 cggttacatt cgtcattttt gcattgcctc ttttatcttt tatgggttct ccattctcat
     4021 ctaattctgc gtcttttgct gctcgcatat atccaaaaac atcatcatct ggatatttta
     4081 aaggattagc ttctgtaaaa gcaattttat tatcacgtat aacaggcgat aaattccatc
     4141 catgtatttt ttgtaatgta tcacgccacc aataacgcca agcttgtccg gatacatata
     4201 cataatttct tccattctta cgaatggttt ttgttgctac tgcattatca aaattgcttt
     4261 gtgtgttttt tcctgcgttg tttaatgcta caacatcaac atcaattaaa ataaatcctt
     4321 gtgttttaag atttttcata tctatatttt ttaataaatt atttttttta gttctctgct
     4381 tcaatttctt caatttcttc agactcgaaa tctactttta gttcttctga aatcagattt
     4441 ctttcatgca tttcttgata aatagcaatt agtaaaaggt cccttatttc agaccagtgg
     4501 gatccatcag ggaataaata atttatgtaa tcctctaatg tcaggattgc ttctttattt
     4561 ccggaattat aattctcagc tattacatca cgaaggatga agcgccttaa tcctgcagca
     4621 tttttgatac tatcaagcgc tctgattcgt tttttaattt tatcggcatt ttcttctctt
     4681 acaagaaata ctgccaattc cttgattttg ttaatagttt cttttttcat actcataata
     4741 ttttgttgat aaatagatac aatttcaaat ttgaatggat gtttgcgaga ccaacgaaga
     4801 atttccggaa taatactctt attcgatatt aatttattgt aaatcctatt ggaccattgt
     4861 ttatattctt caaagtcgat agtttcagtt tcttttgatt ttattaattc gaatgttccg
     4921 gcttgaggat tgtatttggc acttctatat tttttgttat cgaaataatg tgagcgaaca
     4981 aaattttgcc aatcagtttt gaattttggt tgcatgcagg ttcgataaaa taaaaagact
     5041 tgagaaggga gttgatatat tttaatttca ggagaagctc caaaattggt aaaatgatag
     5101 agagttaatg agggcataat atcttcttct ttttttaact cattcagata attaagagaa
     5161 taatcgacaa aatcaaaaat tgcattagcg ggtttattga aatttgattt tgcaattctt
     5221 gttgaggttc cggttgcaat atcttttaaa ttttgatcaa ttgttcgttt aacgaataat
     5281 ttgcttaatt tctcgttact actttgtatt aaacatattt tcccctcgag ctgttgacaa
     5341 acaaaaggaa taaaatacat tcttatcaga gcttctttgg aaagtgaaat tcctttttga
     5401 aagaaatggt gaaaattgat gaaagtgccg gaaccggtca tgatagaatt atctcgacct
     5461 gcaaaaaata attttgtttt ctgtcccgta attctacaat aaccgatttc gtatggttct
     5521 gattcattga ttaaggattc aaagtatttt aatgtttcct cgatttttct tttattgtcg
     5581 aatgccggct gtgtgatctt tgagttgaga aaaaaagcat ttatttttcc atcccatttt
     5641 tcaacataaa ttttcgtaat atattcaatc agttccagaa tgtccttttc gggggatttt
     5701 tctaaaagat attcaatggc aaatcctccg gcatcagcaa aaggatcacc tgtaggcttg
     5761 gttaaccatt tataatctat agttttataa gtttctttca ttttttatct ttttattttt
     5821 tcttaatatt aattttttcc cattctctca ccattccaaa gcccaaactg tttttgctac
     5881 ctactccgcc atcatatgct attttcataa gttctatggg tgctgttaat tgaaattggc
     5941 aagaaaagcc gcgaattttg ctctcttgtg gagtgtcagc tttgatagtt atcagtgaag
     6001 agcgaggttc gttaattact ttaaaatcga aagcaaaatc atgatcaggg aagtccttac
     6061 cttgaaaagc tttatattta tctaatagat tttgtttgat gatgggcata gccattggat
     6121 gagtggggga gatgtattca tcagacccat tatctctttt gagtgtgagg cagataggag
     6181 agagtgtttt aagtgtcatg gtttctttaa actctggtgg tggcagcatc tcgatatttt
     6241 gtattctaca ttttattcga gcatttctgt ttcctaattc aaattcttgt tctctaaaaa
     6301 taccttgaat aaattcgcgt gtactaatct cgggaatgaa tgaaatctgc cattctatgt
     6361 ggtctgataa tattctaata tactcattta tgatgcgata ttgtggtatt tttagtctcg
     6421 aaaaagtaaa tagcttgaat agttttttat ctgcattata gccattatca tgcaaccatt
     6481 gagaaaattt ttcattagaa ttagataaaa ttttgtaaat gactgcagag cattcatatt
     6541 gataattaat aggtaattta tctctacctt tagatttatc tatatttatt attaatttaa
     6601 acctcatttt ttattaaaat tgaaaaatat ttaataaata atcatatttt tgtgtgcgaa
     6661 ggtaattaaa atatttaata aaaaaaaatt tatttaatag aacctattaa ataaaaaaaa
     6721 tggttctata acgttttata ttggttaaga ttaaaaaaaa gaataattta aaaaaagaga
     6781 gagagataat attcccaaat tatcagtaca gagtaatgca tgtggagcat tactttagtt
     6841 gtataaaaaa caaaagtcaa atatctttta tcatattgat ataaatcaat taatttattg
     6901 atatatatca atatgattta ttaattatgt tttatatcat tttgcaattc ttatattaat
     6961 tttgtaaatt aaaattaata aatatgaacg aaataactag acatcccatt ttagatgttc
     7021 ctaaatcaga ggcggtagag tttttattta atggtcaacg agttataggg cgaaagggtt
     7081 atactatagc ttctgcactt catttagcag gatttcctat tcatagtcat agtatagatg
     7141 gcagacctcg aagcttagaa tgtggtatag gcaaatgcag tgcatgtgag atgctcgtag
     7201 atggggtagt gcgtagaata tgcattacca aggtagatgg ggtaaaagag gttattgaga
     7261 tccctcaaga ttatagacct gaacccaaga aattgtctct cgatctacct aataaacttt
     7321 atcatacaga agtagtgatc gtaggggctg ggccagcagg attggctgtt cgagagatat
     7381 taaatgattt tggagtaccc aatatagtaa tcgatagcaa tgaaagcata ggagggcaat
     7441 ttcttatgca aacgcatcaa tttttctttt ttgaaaagca acgtaaatat ggtggtaaac
     7501 gtggtttcga aatagctaaa atgcttgcag gagatgatca tagtgatata ttactagata
     7561 gtgtcgtttg ggatattttc gataataaaa tattagctgt caaaaacata aaaacagaag
     7621 atatattttt tgtaaaatgt gaatatttgg ttgtaggtgc tggagcagtg ccattcatac
     7681 ctgtattcga aaatgacgat ttgccaggcg tgtatacggc tgctgtagta cagaaattga
     7741 tgaatataga gcatacgctt ctaggcaaaa aagttttgac cgtcggagct ggtaacattg
     7801 gatatctgac ttcctatcaa ttaacgcaag ctggagccaa cgtagtagct atcatagaag
     7861 ctcagcaaaa cgaaggtggg tttccagtac aggctaatag agttagacgt cttggtattc
     7921 ctattcttac tggttataca cttgtaaaag ctatccccaa tgatgattat acagctatta
     7981 taggtgctgt cattgctaaa gctgatgagc aatttaaacc catatctggc actgaaaaat
     8041 taattgaaaa tatagaactt attaatattt gtactggttt aataccagat gatcaattgt
     8101 tagtattagg taaggagatt tttggtacca aatgtcatgg agtaggagat gcgataagaa
     8161 taggagaggg tactagtgcc gtattgcgag gcaaacaagc cgcttatgaa atactttctg
     8221 atatggatat tagatatgat tatgatgatt atctagctgt atctaaagaa tatattgata
     8281 gtcagcaaca tccttttaaa gtcttagact ctcccgcaca acctgatgag caacgaaaat
     8341 caaaaccttt tgtcattatc gattgtttat atgcttttgc ttgcaatcct tgtgtttttg
     8401 cttgtcctca aaatgctatt tccaaatcta gcacttctaa tgtaccagtg gtagattatg
     8461 ataaatgtac tggttgcttt gactgtgttt atcaatgtcc aggtttagct ataattggtt
     8521 ttaatattaa taaaaataag atttttattc ctaccgagca ttttttagaa gaaaattctc
     8581 aggtcttttt ggtagataat tatgctaata aaataggaga aggggtgata gaaaaaataa
     8641 tgataaaacc taacaaaact aatgtggccc gagtgagagt agagcattta tttgatgata
     8701 atatcacaaa tattaggggg ttgattccaa aaaatcaata tcccgaacct ttagttttaa
     8761 aagattataa tgatactatt agtttcgatg atccttacgt gtgtcattgc gaagatgtaa
     8821 agtatcaaga aattaaagat ttcgtaggag atagaaaatt tgtatctgtg atggatataa
     8881 aacataatac tagactggga atggggcctt gtagaggtaa gcgttgtatt agacgattaa
     8941 gacagagact atatagtgat ggtatcacac ttgtaggtga tgctactcct cgtggaccat
     9001 tgtctaatca aattttgctt ggcgaattgt atcctcgcac tataaaagat aaaatcattc
     9061 ctatcggtac taaaaaagat atcgaacata gagaagtaaa agtattgata gctggtggtg
     9121 gtatggcagg tagttctttg tttagatatt ttgcagaggc tggtatgagt cctgttttga
     9181 taaattatga aaaaggttct tcttggcgta atatagcagg gggacgacca gctttttcta
     9241 tacccgaatt atctgatatt gctcgacata atcttgaaat atttcaagag cttaatgaat
     9301 ataaaaatat taatttcaaa ccaacagttt atgtcaattt tgctcatgac caggaaacat
     9361 ataaatctct ggaagcttct aagggctggt cagatgttta tgaagtaaaa cctaaagatt
     9421 tcaaaaatga gataagtcct tattttaatc ctacactaga tacttatttg gctgctttca
     9481 ttagtcgcga atgctggcaa gctaatcctg gcaaaacagt agatctcatt cgtagaattg
     9541 gtctcgaaaa tggcggtact atttatgaag aagctattct gatttctgta aataaaaaaa
     9601 ataataaata ctttgctatt gttaaaatga gagatggttc ttatatagaa tttcaaaccg
     9661 atcatttcgt aaatgccctt ggtggtgaag ctgagaaata tgctaatatg ttagatctat
     9721 atccaggtct ttatccagtg cgacatcaag cttttattac tcgtagacta ccttatttag
     9781 gtaaaaatgg taatagcttg gatatgttaa tagatagacg aaacttcaaa ggtttcagtg
     9841 ctgtctatgg tcaacaacta tatgaaactg gacaaatcat tggctgtgct tcaccagcag
     9901 tagattctat ggagtcatca aaggatatca aactcaatac tagagaattt atgtctatca
     9961 tttctgaggt cttttgtgat tggataccaa tgctcagttc tgtacaattt cagagtatat
    10021 ggagcggtta ttatactgaa cctcgcatga ttattgatcc agaaaatggt ttatttgttg
    10081 gtatgcgtgg acatgggttt atgcttagtc agtatttagc taaattgtat gtagataaat
    10141 ttttaggcag aggagttcct tcatatttcg aagagttaaa gctaacaggc aaaggattga
    10201 gtgaaactgc gtttaaataa ttttttttta actgatttat caactactta ttttcaaata
    10261 atagctcaga gataaatcta tactatgaaa cttttgttat ttttttatta cttttgctat
    10321 tagtaatttt taaaaatttt aactaaaaat taatgtctca aaaaatatta acttggcata
    10381 gaaagatttt tgataatatt agcaggcaat atgcagagca gcgtttgccc catgctattt
    10441 tattgtcaca gcgaatgagt gagaatgctt tttttttagc gaaatcccta gctcaattgt
    10501 ttcaatgctc aaaccctcaa ttaaataatg aaggatatct agaagcatgt gatctgtgct
    10561 cgagttgcaa aaaagttaaa gaatttacac atctcgatgt tcattatttt tttccaataa
    10621 ttaaaagaaa aaataaaact tgctcagatg attacttcca ggaatggata gaatatctaa
    10681 gatcgtatcg aggtataata gatctaaata gttggatacg tttcatagat tctgctaata
    10741 gtcaagcaat aatatctgca gaggattgtg ataagattgt aaatatagca caagatagta
    10801 gttacgaggg taaatataag attttcatta tttgggaaat ggaaagactt tttcatgctg
    10861 ctgcacctaa gctattaaaa ataatcgaag agccacctgc taaaacgctt tttgtaggca
    10921 ttacagatca cctagagcag gtattgagta ccatcagatc tcgcatgcag ataattcaga
    10981 tacctacacc tactccagaa gagtctttgg agatacttaa tgattttttt gatattgaaa
    11041 aggatgaggc taagagattg gtttttacct atggtaataa tttacctcgc atagtagaca
    11101 atatccatac agataaaatg cctattgcga ttagtgattt ttttattaaa tggatgcggt
    11161 tgtcttttca gttaaaagct cacgaactac tcgatttagc tattgagttt aattctttag
    11221 gtcgtgatat tcaaaaaata accctttcgc agtgtttgaa ttatctagat gctatttggc
    11281 tcatcaatca taatattcct actcagttga tactcaatga gttggatgag aaatttgtgc
    11341 atgattttaa aaaatttatt gatgatgaca ctatagctga ttttgcatgt tatgtagatg
    11401 aagctatcaa aaatattgaa cgtaatgctg atagtaaaat agtttttttt gatttaagct
    11461 ttaagatagg gcagaccttt aagcagaaat atatgatgat gaggtaaatg tttatttaat
    11521 aaaatggttc tataacgttt tatatggatt aaagattata aaaagagtaa ttttataaaa
    11581 aattgttaat attagtttat taatttatag tgtagttaaa tattaaccac aaagttctca
    11641 aagaatgcac aaagaacaca gagaaggatg gaattaaaag ggctacaaat gggactttgt
    11701 ggactttgtg taataacttg gtgccctttg tggtaaaaaa ataacctaaa taattaaaga
    11761 tagttatttt tttgtaataa tctaaaattt tattactcaa taaacgttat agaaccaata
    11821 aaatattata aattaatctt tagcaggttt ttaattatca gatttatgtc atttaagacc
    11881 atttatattt ttaaaaattg ttggctaagg ttactatttt ttatcaaaag cccataattg
    11941 tatctttcta ttttaacttc aaaaaaatca atttttttaa tatttttcca ggcactatac
    12001 attgaatatg aataattgat atcatctaat ataactattg acaatggtgc gattatttta
    12061 gtaattttat ttattaaatt taaagtagct tcataagtat gtgagccatc tattattatt
    12121 atttcaaatt taatattttt actaataaat tcatctatcg cttcatcaaa ttcgagatta
    12181 taaacgtgta aattgtcaat ttttaatcta ttttttaatt ttaataattg cttacagaga
    12241 tcatctaccc cttctatggt ataaacctct aaatcggtgt tagacattgc taatagagca
    12301 gaagtgaaac caaagcctcc acctaactct aatattttat ttgttttaaa atattgagct
    12361 aatcctatga taaataagcg tttgtttaat gaacaagaaa tttttctatg ccaataactt
    12421 atagttgttt caatgaatct tttattagca cctaaagact gaaatcgtaa tggctcattt
    12481 gagtgtttta atttattttc ataatctaaa agttcattag gtatatcata attcgtttta
    12541 tatgcattca aatacatatt ataaagaaaa ggtgaatgaa ttttaatagg atttttacgt
    12601 gttattatat aattaatcgc atggcaaact ttaaaaagac aatttttcat ttcaattttt
    12661 ttttttgaaa gttagctatt ccatggattg tactttaatg caaaacttgt gaatcagctc
    12721 atatatttct tctaaataat ctggaggtag atttgctttt ttagctttag taataatttt
    12781 attttttttc tcattatatc tctcttgctg atatagaggg atattaagtg agtgctttag
    12841 tttgccaatt tctttacata gttcgtatcg ctttattagt aaattaaaaa ttttagtatc
    12901 taattcatct aattccaatc ttagtatttt taatctatca tctagtgaca tataaaaaaa
    12961 ttttagagaa tgaaaaactg aataattata agtaatgtat taaataaaga aatatacggg
    13021 tttatctctg ctagagcttt atctacaaat tttttctttg gtagtacata tatcatatct
    13081 ccatctctca aatatatgtt acctaaatat aaattttctt tatcttgcag attaatttta
    13141 taaaaagcaa agtcttcttt gtttgtatca gtagctctgg ctattattat tttattagaa
    13201 taggcttcgt ttcctatccc gcctgtacta gtaatagcat ctagtagtgt gatgtatgaa
    13261 ttagaaaatg ggacagcagt accactacca ctaccttgtg aaaaaacata tactttttta
    13321 ttggcccaac ctaaaaaaat gtaaggatct gtaatatatg aagagtataa ctttgtcaat
    13381 gtatctttta gagtttccaa tgtcaatcct tctacaaata tattaccgat cagaggcaaa
    13441 ctaataaaac catgttcgtt aacataataa attatattgc taacttcttg attgatcctt
    13501 ctaatgtcgc tttcatagat taaaagtgct tctccattat taggatatac taagatattg
    13561 aactgatctc ccttttttat agtttgtgaa ggtgctactt gtagctcttt gagatctctg
    13621 aaatcatttt ctgtcagatt tgtcattaat aaattgggat aataataact actacacgac
    13681 gttaatgata ttaataataa aattaaaatt aaattataaa acttcatttt gaaaatttaa
    13741 aaaatttatg caaatttaaa agtaaaaaat taactgaaaa ttttaaatct aattatttag
    13801 ttttttatat taaaaaaatg ttattagtaa gaaaattaaa tagatggtat ttttcaaaat
    13861 tcacattcaa aaatctgggt ttaaaatttc actttagttt tttcaatatc tttgatgatt
    13921 tcatgtataa tatcagttgt attaacgcct tcggcttcgg ctctataatt gagaactaat
    13981 cgatgtctaa acacactatc tgctacagct tttacatctt cgttgtcagg agcaaattta
    14041 ccatttagca gagcatgaca tttagctccc aagatcataa attgactagc tcgtggtcca
    14101 ccgccccatg ccacatattc ctttgccata ggatgactat cttcataaac tggcctagtc
    14161 attgctgtga tcttaacagc aaattcataa acatggtcag gaacatttat ttttcgtact
    14221 aatttttgat attctaataa agtgtcttta tcgattatat tattaactgt aggcatatct
    14281 acgaaggtag tacgtttcac tatttcgatt tcttcgtcta tagatggata atctaaccaa
    14341 atagaaaaca taaatcggtc tagttgggct tctggtaatg gatatgtacc ttcttgctct
    14401 atgggattct gagtagctaa aacaaagaaa ggacgatcta aagtaaaagt atttccagca
    14461 atagatacag agcgttcttg catagcttct aacagagctg attgtgtttt gggaggggtt
    14521 ctgtttattt catcagccag tattatatta gcaaaaatag gaccttttat gaaatgaaaa
    14581 tttttatgct catctaatac ctctgtacct aatatatctg atggcatcaa atctggtgta
    14641 aattgaattc tattaaagga taaccccagt atttgagcaa tagtagttat gagcagggtt
    14701 tttgctaatc caggaacacc tattaataaa gcatgatttt gtgtaaataa agctattaat
    14761 agcttgtcta ttacatcttg ttgtcctact attactttag cggtttcatt ttttaaattt
    14821 tcataaaatt ctactaaact tttagcagct tcagtatcgc tattaaatct cataagttaa
    14881 tattttttaa tccaaggatg tttgaaatca caaccatgat attctatatc tattttaacg
    14941 taaacgtctt ttagtctatc tgtaacccat ttttctaagg cttgcctttt tttctcttct
    15001 aaagcttgta attgtattag aaaataatct gtcttgagtg atgccttatg tgggggaata
    15061 taatttttta accataatat ccgatatgtg gggctgccgt cagcttctaa ataaagaaaa
    15121 ggtttagaat atttagaatt tttcatagta tctaatatag gaaaatattt aggatctata
    15181 tcattagatg ttaaagtaaa tccaccacta ctagataaaa tcaaacctcc ctgagttagt
    15241 tcgccctcga gatatgaata ttttttagct gcttcttcaa aagtgatttc attattgatc
    15301 aataaattat agatactatc tgctttttta ttagctaact gtatagattg attagaaggt
    15361 tgtttgcgaa taagaatatg cctcacattt acttgttctc ctttgcgatc tatcaattgt
    15421 aaaagatgaa atccatagga agtttcgatt ataggtgata tctcacctgg tttagtaagc
    15481 gaaaaagcag tattttcgaa actaggttcc attattccct ttgtaaaata tcctgtagcc
    15541 ccaccatttt tagcagttgc gggatcttca ctgtataagc gagccattac ttcgaagtct
    15601 gcaccatcct ctatcctttt ttttatatct tgcaatttag aaatcaatat atttttctct
    15661 tcctggctca atgtaggttt gatagctatt acacccaatt cccattgtgc aggtatgata
    15721 ggtatagagt cttcgggtaa ttttttataa tatttctcta cctcactagg tgtaatattc
    15781 acattagata ataatttatc attcatagtc attattagta gttgttctct aagaggttca
    15841 cgtagctctt ctttaagttc taaaacagtt ttttcatagt atttttctaa ttcttgtata
    15901 ttgccaaata atgttgtgaa atatctaata cgtctatcta gttcattatc tagctgctca
    15961 tctgacactt ctatagagtc tatctctgct tgcatgagca aaagtttctg caatagcatc
    16021 tcttctaata tttcacaaga caactcatca gttcttatcg atttatctat ttcccccgag
    16081 agaagcattt gctgcacata catctctatt tcacttttta atattatttt attgcctaca
    16141 atagcaatga ttttgtctaa tacttgaggt ggttggctct gagaaaaaag tgaaataaaa
    16201 gaagcaacaa ttatgaataa agctaaaaaa atttttttca ttatttcaat ccttatataa
    16261 agatctcaat acatcttcat tgatttgcca ttggtatcta tttctgagtt cattgatcca
    16321 atttctttct aaataagttt gatattcaga aattatcaaa cctctaattt catttaagct
    16381 cttaggctga ggagggatga tctgttttaa ccaataaatt tcgtatatat catctttagt
    16441 tttagatgct agggtattta ctactttttc tattccaaga tcagctaatt tattcgtttc
    16501 tactaccata gtatcgtaat caacaatact atctttttta tttattttag tcaaaatatc
    16561 gttgggattt agattagatt tttttaatcc tttttgtaaa gatttataaa ctttatcggc
    16621 aattttttga ttattacatt tgaatactgc taatacagct ttttcttctg ttaaataatt
    16681 atgtttaatg gtttcataat atgcttttag tcctgcagta tctttcatag ctttgctcca
    16741 tatattctga tccatcaagt tgaatatcag aataccttct ttatattcat taatttgttc
    16801 ggcaaattta ggatttttga gttctaaatt ttctttttct atttctaata tttttttatc
    16861 taaaaattta tcaaacattt tattgacaaa tacgtgcaca tcgtctgtat tgctaaataa
    16921 gctttgatta cttttaataa aagttgcaaa ttcgctaaca gcaatagaat aatcatcaat
    16981 ctttacaaga atattatcta aattagcaat ttgtgattct ttaaaattat tatttattaa
    17041 agaatctttg ttaatagctt tagctatatt tgttaatgat tttttatcgt aagtaatttt
    17101 ataaacttct cgtaaactat ctattattac tttctgagca atctccatac gtgggtctct
    17161 atttagtttg actaaaagct cttgctttgc ttgagaaaaa tcgggaggtg tctgtttatc
    17221 tataagtttg ataatatgcc atccataaaa agttttgacg ggcggtatga tatcgcctgg
    17281 tttatgatca taaataggct ctacaaaagc aggtactaat ctaaaagggg ataaccatgg
    17341 taattctccg ccattaatag ctgaaccctt gtcatcacta tattttttag ctaactctga
    17401 aaaattttct cctcttacta aattttgata aatactatct gctcttaatt ttattagttc
    17461 ttttgtagtc tcatcagcat ctgggggata cattagcaaa atgtgggcta ctttaaatct
    17521 atacaaagca ggacgtttat cattcaattt aacaatatga tatccaactt gagtacgcac
    17581 tggtgtagaa atctcgccaa tttttagatt atatgcagca gtttcaaaat cataaatcat
    17641 attaaagaca ttaaaatatc caagatctcc accattacca gggattattc taccatcagt
    17701 agctgtttgc tctcgagcac tattatcatc actcatctct acagctaatt tagcaaagtc
    17761 cataccactt aataattttt ttctaatgtc taatgccttg ttataggctt ttaaagtatc
    17821 tttaggatta gcataagggg taatctttac aaggatatga ctagctctca cgtcatgctg
    17881 catacgatta taagcttctt gtaatagatt atcaatagtt tttttgtcta tcaggtaagt
    17941 ttgagttaat tggtccctat acctttgaaa ttcattaata aaagtttttt gagtgtctaa
    18001 tctcagtctc tgtacttcca ttagtttgag tttgtaatta ataaataagt ctaaatattc
    18061 atcgatacta gcttttgaga aatcaggatt tctattgttt ttaccataaa cttccagaaa
    18121 ttctgaaact ggaacttctt gattttcgat tattaaaaga attttttcat tttgtcccat
    18181 taatgtcatg gacaataaca aagaaaaaat tacaatacca atatactttt tcataattta
    18241 aaaaattttt aataattaac gagaatagtt gggagcttcc ctaataatag taacatcgtg
    18301 ggggtgtgat tctataactc ctgcattagt aatacgaata aatttagctt ttctaagatc
    18361 attaatattt ttagctccac agtatcccat acctgctctc aagcccccca tcatctgaaa
    18421 aatcacttcg gatatattgc ctttatatgg gactctacct actatacctt caggcacaag
    18481 ttttttaata tcttcttcca tatcttgaaa atatcgatct ttagaccctt gttgcatagc
    18541 ttcgatgctt cccatacctc tatagacctt atatttacgt ccttcgagta taatagtttc
    18601 tccaggtgct tcttctactc cagcgaatag actacctatc atcacactat tagccccagc
    18661 agccagagct ttaacaatat cacctgtata tctaatacca ccatcagcta taataggtat
    18721 acccttatct ttcaatgcat tggcagcata aataatggca gacaattgag gtactcctac
    18781 tccagctatt actcgagtag tgcaaataga accgggtccg atacctactt ttacacaatc
    18841 agctccggct tctgctaaag ctaaagctcc ttcggtggta gcaacatttc cagcaatcaa
    18901 ttggatattt ttaaaggtag atttgatttt tttaatagtt tctaacacta tagcggattg
    18961 tccgtgagca gtatctacca ctatagcatc cgcatttaca gaaagcaatg cttctactct
    19021 ttctaatgta ttactactaa tgcctacagc agcagccaca cgcaatctac caagttcatc
    19081 tttacaagaa ttgggtctct cacgaacctt cattatatct ttgtaagtaa tcaaaccaat
    19141 taatgtacca ttattatcaa taacgggtaa tttttctatt ttatattttt gcaaaatagc
    19201 ttcagctgtt gtaaagtcag taaactcact tacagtgatt agattttctt ttgtcataac
    19261 ctcagagata ggtttagaat aattattttc gaaacgtaaa tctctgtttg tgacaatacc
    19321 aattaattta ttgttttcat cgactactgg aataccacca atatgatttt cgcacattaa
    19381 tttcaaagca tcttccacag tattatgtgc aaatatagta ataggatcga tgatcattcc
    19441 attttctgct cgtttgactt ttttgacttc tttggcttga tcttctattg atagattttt
    19501 gtgtattaca ccgataccac cctcctgagc catagagata gccattttag cttcagtaac
    19561 tgtgtccata gcagcagaaa taagtggtat atttatcttg atatctctag tcaaataagt
    19621 agtagtatct acttctcgtg gcaatacttc actatatgct ggcatcagta aaacatcatc
    19681 aaaagttatc ccttcattta ttattttatt tttaaacatt gctaaaaaat atttatacaa
    19741 aaatacaatg aaatttgaaa ttgaaaataa atagttttta caaaattatc aaaattatca
    19801 aatagtttta tattaaattt gcaaaaaatt tatataattt tacttttatg ctcgaagtta
    19861 ttttaggttg ggacaaagca ttattattgc ttatcaatca tgccaggtcg gaatttttag
    19921 atgttattct atatttagta tcacagcaat atttttgggt gcctttgtat gtaattgtac
    19981 taatattatt aataaaattt tacaaaaaaa aagcttgggc atatatatta ttatttttca
    20041 ttttattatt atttacagat caatcttcag tttttataaa aaataatgtt aaacgattac
    20101 gcccctgcca tgaacctgag ataaaaaatt cagtgatttt ggttaaaaat aaatgtggtg
    20161 gcaaatacag ctttgtatca tcacatgcta ctaattgtat ggggtttgct actttagcat
    20221 ggttattatt aaagcgtttt gctaataaaa aatggaaaaa aacactttta gctatagtac
    20281 ttttcggttt gtatgtattc actgtgatat atagtagagt ttacttggga gtacattatc
    20341 caatagatgt gtttatggga acaatattag gtgcactttt aggatatttg gtttatttat
    20401 ttagcgaacg attttttcct aaattatttg ccttctcctt taaaaatgat aatgatagtt
    20461 aaaatcagaa ttttaaaatc actaaacaaa gaaatattat ctaaatataa aaggtcataa
    20521 taaagtcttt cgaccatctg atctacattt tctgcatagc catactttac ttgtccccaa
    20581 gaggtgatgc caggttttaa ttttagtagt aagcggtaat aaggaactat ttttactatt
    20641 tgatcaatgt aatattgtct ttcgggccta ggacctacta atgacatatc acctttaata
    20701 acattgaaaa attgtggcaa ttcatcaaat ctatattttc gcatcacttt accccatggg
    20761 gtaactctgt catcgtcttt acagctcaat gcagggccat tagcttctgc atcaatatac
    20821 atactgcgaa atttatagat attaaatggt ataccgtacc ttcctatgcg ctcttgcttg
    20881 taaataattg gaccagggga acttttttta acctttatcg ctatgaagac caataatggc
    20941 gaaagtaata taagtgccaa ggtagagata aaaatatcaa ttgttctttt taatatgctt
    21001 tgccatgtag acaataagta tttcggcact tctatatatg caatattaga aaagggatgt
    21061 atctttacac tactagttac taaaatattt tttacatctg atactatata tatgtttaca
    21121 ttgaatattt ccctaataga gagaacattt gaaattttgt cataatcagc tggttctaat
    21181 actatataaa tatcttcgat ataatatttt agaataatat ctcctatatc atctaattta
    21241 cttagacaag gtactatttc gtgcaaagta tctttgcctc cattgatctt gatgtatcct
    21301 actacgtgtt gaccagtatc ataactattt tttaatatgg attgataaat tttttctgct
    21361 aaattattat tacctattat taatgtattg aatactattt tctttttttt aataccattt
    21421 ttggtccatg tcatcagaga taagcgtaat atatatgtca gagctatact cactccgaaa
    21481 tatgaaaaat acacattata caaatatttg taatgataat aaggataatc atcgagtact
    21541 actataaaaa atataattgt aactcctatc aagcattgta aaacagtatt aaataaaacc
    21601 tttaaacgac ttttgcgata tacttctcga tatgaatcag ataaagcaaa tataccaatg
    21661 aaaataaaag gtatgattat aactcctttg tagaaattaa tatcattgaa aatcatatta
    21721 gacaaagtgc tgaagtcata aatcccctga tacattaaat tttttcgata aaaagacaaa
    21781 aacaaccaac caataatact tgatattaga tctaatagta gatatctagt agtatgatgc
    21841 attttattta atggtggttt cattttattt ggctcaatca ttctgatgat attaatttta
    21901 tattatctaa taatatttta ccttcactat ttgtactagt tttatatgta gcaaagaaga
    21961 taataaaatt actattttgt ccatatctac ttgctaatgg cgacagattt atatatattt
    22021 ttttccatat atcagtaggt tttaatatta cataaggata agtaagtggt ccagtgttag
    22081 tataggtcat gatacccact gttaaatttt cggtactttt ataattaagc tctaaaaagt
    22141 gcattaagcc cggagctaat ttatagctat taatagtagt gataatagct gtatctctat
    22201 catcagtcaa agatatctta ccagaaaaat taccttcgaa tacttcagtt gcatcgtcag
    22261 tatttatcat aaaggtatca ctggtagaat ggctagtaaa aatagaacct ggtgtttcga
    22321 aatcttcaat aaaatgaaat tttatacctt ctacataatg gtatactggt actaattcta
    22381 tctcttcaag tggtgataaa tttatattta ccttataatc atcataaaat ggatatactg
    22441 ctctggtggt agatatgcca tttagtttga tgccaggcat gataatcact tcattacttc
    22501 cttcaaataa taagggaaat ttggctggta attcatatac tccacttcgc tgtccatcta
    22561 catataccca tactgtattg atagcatgtg aagctgatcc ttcgccgggt ctcacattga
    22621 gcttcacgct atctattttt atataacaag gtaatttttc atcaggatct aaccattcac
    22681 aagatgtgaa aaatactaca aaaaaaatta aaaaaattaa agagataaga tttttcatac
    22741 aataatttaa gattttaata atttattaaa acttattatt tgtaatttca aattagctat
    22801 gtttatttgc atactaatag agttattttt ttgtttacta atcatcaatg accaattatt
    22861 aaatgtatat agtattttta ccatttataa ttatttaaaa atcaacattt tgctattaaa
    22921 acttaaaaaa tcataaataa ttttattaaa gtttcattaa tatattaact ataaaatatc
    22981 aattttatta caaaaaataa ttaaataagg ttagacaagt tatactgtat ttcattaatt
    23041 attttataag ctacatctaa tactttatag ccatcatcaa tggttacttc tggtttaata
    23101 cctaattcta tgctttctaa aaatttttct aattctgttt tgatagcgtt tatttctggt
    23161 agattgattt ttttcactac tacatatttt aaattatcgt tatttagttg tataggaaat
    23221 gaaaagacgt tgtcattagt atcatttttt atattatctt ctattttgat aacttcagat
    23281 tgtttattta ggaaatccat actgatataa ctatcttgtt gaaagagtct cattttccgc
    23341 atttttttca ttgatattct actagcagtt aaattagcta cgctaccatc ttcgaattct
    23401 attctagcat tggctatatc tatattttgc gttaatacag gcactccatt agcatatata
    23461 cctttaatag gtgaatcgat aatagtcagc agaatatcta tatcgtggat cattagatcc
    23521 aaaaccacag gtacatctgt gccacgcgga ttgtaataag ataatctgtg agcttcgata
    23581 aatttaggct cattaatata tgaacgggta gctaaaaaag ctggattaaa tctttctaca
    23641 tgtcctattt gtatttgtac accagcattt ttactgattt tcattagttc tttagcttct
    23701 tctaaagtag taactatggg tttctcgata aaaacatgct ttttagcttc taaagacttt
    23761 ttagcatatt cgaaatggga tactgtaggc gttacaatat ctattacatc cactttttct
    23821 aaaagttctt caaaattttc aaaggccggt atattatatt tttgttgtac atcacttgct
    23881 accaatggat taacatcata aaaacctact agatcatacc ctgatatttc ttttatgcaa
    23941 ttgatatgaa tttttcctaa atgtcctgct cctattactc ctatttttcg catttttttt
    24001 gcaaagataa attaataaaa ttgaattaga cgtctagtca ataataaaaa tatagttcta
    24061 taacgtttta tatgggttaa agattaaaaa aaagagtaat tttataaaaa atttgttaat
    24121 aattggttta ttaattatag gataatcaaa tattatccac aaagttctca aagaaggcac
    24181 caagaacaca aaggaagatg gaatcaaaag ggttataaat ggcactttgt gaactttgtg
    24241 taataattta gtgccatttg tggtaaaaaa ataacctaaa taatttacac acaataaacg
    24301 ttatagaacc aacatatata aaaaaattct ttattcaatt tttttatata aatttgtatt
    24361 tactaaaatt aaaacttatg acaaaataca taaaaaacaa tatttatgaa ttaaaaattt
    24421 atactattaa tttcagatta taaaaaaata ttaatatata aatttgcata attataaaat
    24481 taatatataa atttgcataa ttataaagta aataatccct taaaatataa aatatatgaa
    24541 tgtaaaacaa attttatcaa tttcaatcat gttgctctgg gctacttttg cctttgggca
    24601 gctacagggt ctagtagagg taaatcctac tgaccctgtt tatcctacat tatctgcagc
    24661 tattgattcg ctgaataatc aaggtgtagg tgatggtggt gttactatta cggtagcacc
    24721 tggcaatcct cagacagctc ccgctggtgg ctatgtgata actgctagtg gagatatatc
    24781 taaacaaatc attattgatg gtaatgataa tatcgttact gctttctctc cacaagatga
    24841 aggtaaaatc aatgatgcta ttttcaaaat catcggtggc gactggatta ctattaagaa
    24901 ttttgttatg atggaaaacc ctgctaatac tattacgact gcttcatcta ataacatgac
    24961 tgaattcggt gtcgctctat tctacgctac tcctgatgat ggacctcaaa actgctctat
    25021 catcggtaat actataactc tcggtggacc ttatcaaaat accttcggta tttatgctaa
    25081 tagcagacat actgctacag ctatgacaag tggagctgat attactagct tagatggtgc
    25141 ttttaataat cttcaaattc taaataatac tatttctaat gtaaatatgg gagtagtgct
    25201 cgtaggctct actaccggtg actatatggc tagaaatatc gttgtagatg gtaataatat
    25261 tacttatggt tatactggct ctttctcgaa ctattattct gtctctggca ctgtgaatgg
    25321 catcttgctt aatagtatag tgaatgctac tgttattaat aataaactca cctctgataa
    25381 tactgtgact gcaggtactc tacgtggtat ctatcattat gtgagtggta caggcgctgc
    25441 tctacctact agctttgcta ttgttaataa tttcaataat aataatattt tcttaaaaag
    25501 aaatgcagct aatgcttcta tctatgggat acatctagat aattataatg atagtgtaac
    25561 aaactatgtg atagctgata ctatcagagg cttgggttct gctgctacat atacctcagc
    25621 agtttatggt atctatcatc aaggagctgc taaaaatcag tattttaaga aaaatatttt
    25681 tcaaattaat acaaatactt caggttctgt atatgctatc aatggtggca atactataaa
    25741 acctaatggc atccaaatgc tagatagtaa tcgtgtagat tatctaggca aaactgtcgg
    25801 tggtggtaca gtttatttct attattctaa tagtagctca gactctactg taaaaaaata
    25861 ttttactaat aatgtgatag atagtgttac tttaactgga gctatcactt tctatggata
    25921 ttatgataga gatggtgggg cttcaagtgc agcaaaaaaa tattttaata ataatctaat
    25981 aaaaaatatt actggcggta caagtgctgc ttatggtttt aatgtgacat atggcaaggt
    26041 aatagatatt cacaataacg ttattcaaca attaactaat ggtggtaata ttaattgtat
    26101 ttatgctggt ggctcttcta ctctggatat gaaaatttat aataataaaa tacaaaatat
    26161 aactactact agtggtatta ttaatggcat ttattcagca gcaaatgcta ctggtgctat
    26221 tgctaatata tataatgata ctatcactga tttgaattcg gcaggtagtg tttatgggat
    26281 tcaaatttac agtggaatta ccactaatgt gtataataat ttattatata atttaaattc
    26341 gagtggtact actggcactg tttatggtat ttatgcttat ggtggcacta ctaataacat
    26401 atataataat t
//
LOCUS       DAOPRN010000025        26033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1509_length_26033_cov_131.062283, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000025 DAOPRN010000000
VERSION     DAOPRN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26033
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1509_length_26033_cov_131.062283"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PKI65_04350"
     CDS             <1..929
                     /locus_tag="PKI65_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77705.1"
                     /translation="PATSLLVYNTATTGDVTPGYYYWNGNQWVRFATGNISGLINCSS
                     TNYLIKSDGVNGVCTDIIENANKNIGIGATPNDNYKMYVYRPSTSYGAGKAGIYSYRY
                     GTSGSTNGGTSWSNTGIDAAIKAYSYWGNEYTAAIAGYSYLDYTNSAAIVGANSGGTI
                     RAELATKTPTYDGSTSDGTYSLKLTGDFYNQDINYSQSTEVYFTGTTLRSVNVTTHGT
                     GVNNSIVWIHGECDYTKDDATSFVCFYIYRDNTLLCEVSEVSYVDEDKTVHVQWIDEP
                     PAGPHTYELRVYYPNGGMTYYGHQLHVVELKR"
     gene            934..1455
                     /locus_tag="PKI65_04355"
     CDS             934..1455
                     /locus_tag="PKI65_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77706.1"
                     /translation="MKKLFLVNAILFVCLILNGQNNLNDDAKARIGTYLKINPQILNV
                     QISSLEAKSTVDIIVFAFELYFIIPTVSLNNIKDLQEKKEIELLISRLDNLLKNPPEW
                     EHIIALQNEKKSKPPTPPVYAGDEKAKQYYNTKYSDLNKKSSGSKGMLIQNETIIEPP
                     VPIPDKSKELINE"
     gene            1724..2179
                     /gene="rplM"
                     /locus_tag="PKI65_04360"
     CDS             1724..2179
                     /gene="rplM"
                     /locus_tag="PKI65_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOB77707.1"
                     /translation="MDTLSYKTVHLNAETVKKEWLVVDAAGQPLGRLASRVAYVLRGK
                     HKPGYTPHVDCGDNVIVINAEKVVLTGKKMSQKMYYHYTGYPSGQRSEAAKDILKRKP
                     TFLVEHAVKGMLPKNRLGRKILKNLYVYAGPEHLHQAQNPKIIDINTIK"
     gene            2186..2581
                     /gene="rpsI"
                     /locus_tag="PKI65_04365"
     CDS             2186..2581
                     /gene="rpsI"
                     /locus_tag="PKI65_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOB77708.1"
                     /translation="MDNKMISGRRKEAIARIRLLKVEDGKQEITINNRDYKEYFPSIS
                     LHYIVEQPFKLTETWGQYSVKGRLNGGGKKGQAEALRLAISKALVEENPEFRQILKKY
                     KLLTRDPRMVERKKPGQPKARKKNQFSKR"
     gene            2688..3521
                     /gene="rpsB"
                     /locus_tag="PKI65_04370"
     CDS             2688..3521
                     /gene="rpsB"
                     /locus_tag="PKI65_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007135301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOB77709.1"
                     /translation="MEQLTFDQLLEAGCHFGHLTRKWHPNMAPYIFMEKNGIHIIDLN
                     KTLAKLEEAKQAAYNLARAGKKILFVGTKKQAKDIVANAAKSINMPYITERWPGGLLT
                     NFVTIRKSIKKMQQIDRMMNDPSFSNISKRERLQLARQRAKLEKTLGSIANMVRLPSA
                     IFVVDIVKEHIAVSEARKLNIPIFAIVDTNANPQLVDYPIPANDDAAKSIQTILENFV
                     EAIKKGQQERIGAMEEAEREDEEYSEEKLKEKKIKVMEASADAEDDEKSSKKHRTRKK
                     E"
     gene            3526..4359
                     /gene="tsf"
                     /locus_tag="PKI65_04375"
     CDS             3526..4359
                     /gene="tsf"
                     /locus_tag="PKI65_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOB77710.1"
                     /translation="MANISAKDVYKLRDLTGLGVMDCKKALEEAEGDFDKAIEILRKK
                     GQKVASSRADRAANEGIVLTKIAKDKTFGIILMLNCETDFVSKNEEFINLANNIADIA
                     LQNKIKSKEDLLSAKFNGITVAQNINDYMGKCGEKIELSNYEFIEAPLVLGYNHHNGR
                     VAALVGLSKASEKIEEMGHNLAMQIAAMNPIAIDKTDISQDVIDKELDVARELARKEG
                     KNEDLIEKIAQGKLNKFYQENTLLNQEYIMENDKNVIDYIKSIDPETKVIKFYRFALG
                     S"
     gene            4742..5551
                     /locus_tag="PKI65_04380"
     CDS             4742..5551
                     /locus_tag="PKI65_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HOB77711.1"
                     /translation="MKIYAITLFSNKERYNHINSHLKKLHLDYKFIEAIDKKDISDDD
                     MKLYYNISNINELPDFINKGNTANKISKLRAYKDFLSSNEKVAFFIEDDIILPHNIKE
                     ILDKIEKEINDNEVILLDYRTVNTSYKIGISRANSKKINNGLLAYPTQLLGLVGSGAY
                     ILHKNVAENIIKLDLYKNNFSDSWNDFYQKDAFDQLRVLYPVVTKFKPFSSAIGYIPS
                     GSLKYKIKNFIERYKIPFLYQLLIFKRKYFLRSVAKQFFLTDEISPIDKIK"
     gene            5699..7144
                     /locus_tag="PKI65_04385"
     CDS             5699..7144
                     /locus_tag="PKI65_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77712.1"
                     /translation="MRHSFFYVLLFISFVGFSQNAIIYKSFDNYSFLNTNDRDSTKYL
                     ITPYFSGLASVDNYEVMIGAHIKKRFQNKNTNFMVWTKPEFLYFSLFDYLNDYDSISR
                     FPGGEKTFNNQYSWNAPIELTMNVKNHYILSAGYSKFHWGDGYRSLNLSYSSANYPYL
                     LQQFKIKGLEFSWVMMRWTNPLNKPNFAKYSIFHSFSFNIGPKWRFAFYENIIYPARD
                     SNHHHLEYVYLLPHIFYRYVDYSLGSPYNLLFGGNFNWNVGKKFQIYGQATLDDFIFS
                     EFRKDIKHLIHPTDSSIVYGNWMNKYAVQLGIKWKNKNNKIRTRFEFNAVRPYTYSHK
                     NPIQNFSNNGQAVAHPFGANFYEALLEASFIKHNWHIYLISFYKHIGYDTTGTHFGQN
                     IFYPTFDAYMGNNIPVNPFYNVIGQGCLERLIAAQINFIYKVKIDYVNHMYFGVGFEY
                     RYSINRDHLDFLPFIKLQFNTDNLHVWHTYK"
     gene            7284..8282
                     /locus_tag="PKI65_04390"
     CDS             7284..8282
                     /locus_tag="PKI65_04390"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77713.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRKNVIKHKEIVTSNRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELINITYVN"
     gene            complement(8955..9956)
                     /gene="murB"
                     /locus_tag="PKI65_04395"
     CDS             complement(8955..9956)
                     /gene="murB"
                     /locus_tag="PKI65_04395"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOB77714.1"
                     /translation="MNILRNLSLKDKHTFHCECYADIFIEIEDSDDLIKLNKQYPDIY
                     KKAVFLGEGSNMLFTKDTISFVIHSVIDFIKIINEDKDNIVITAGGGTQWDKLVSYCV
                     ENEYWGVENLSGIPSSCAVAPIQNIGAYGAEFSSVLQKVYGFNFKKCDFEIYSSDKLD
                     FGYRKSLLQQMPEFFITKIEIKLKKRANPNLSYNDLKNKFQNYNPSITEIRQAILDIR
                     DKKIPSPDKIGNAGSFFKNPICSYDKISDLRSKYPDISYYPYNNDKYKISAGWLIEKA
                     GWKGYREGDAGVSSTHSLILVNYGNAKGKDILILANKIINSVNEQFGIMLTPEVKII"
     gene            complement(10003..10782)
                     /locus_tag="PKI65_04400"
     CDS             complement(10003..10782)
                     /locus_tag="PKI65_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HOB77715.1"
                     /translation="MILKHLPERTKKIRKTGITMVMDKGLSPIEAEGLMEVASDLIDF
                     VKLGFGTSIITQNLEKKIQIYKEAGVKVYFGGTLFEAFVIRKQFSDFIKWVKKYKIDT
                     VEVSDGSIALEHEKKCEYISILAKDYLVLSEVGSKDESIIVRPNLWVKEMKDELDAGS
                     TYVIGEARESGNVGIYTKSGNARVMLIEKIKKELPVDKIIFEAPQKNQQVYFIKEFGT
                     NVNLGNVAYQDVIALETLRLGLRGDTFFDFLPENYEEYKQK"
     gene            11026..12867
                     /gene="asnB"
                     /locus_tag="PKI65_04405"
     CDS             11026..12867
                     /gene="asnB"
                     /locus_tag="PKI65_04405"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOB77716.1"
                     /translation="MSSIAGIYSINKDDFILSINKSSVIFVNDRCDRQNYEFVNSKLI
                     LIDSKTSNYNQSVNQRIWDHTNRYVLIFDGEIFNHLELKNKLINLGYNLATNSTLEII
                     LYFLIHYDVEALESFNGVFALAFYDTVEDKLILARDRFGSKQLYYYFDKNSFYFASDI
                     KTLRYINLPLNWDYSSLTLYFFLTYIPSPFSAFEGVNKLLPAHYLIYNGRDIMIKKYY
                     ELPEQIDDVNEIKECIDSIDFLLKQSIEQRLNIDLPIGTFLSGGIDSSIITYLAHEFR
                     PDITAYTISFPDLPYYDESDIAKQFALDNNISHQIIPIVENDLINSISNVLYCMDEPF
                     ADSSIIALYSLVEAIKPQAEIMLTGDGADELFGGYNKYRAWARLHKRNFLNSLIISLV
                     NTIPFHIYDTSIYLCNKLRQLQRFSKAYQLTDFDRYFYLSSMNDVFFVQKILNPELLN
                     DKSFKEKIDFLKNIYYNENKNILSIDFKFVLEGDMLRKIDFVSSDNNVLLRSPFLDYR
                     LVDNVFKIPESYKFNAKTNKILLRDLYKNILPDYIINRPKHGFEVPIRKWMNDFLLDD
                     LIKKGLNVNLKPLLNDNVIKNQNKLYKFANKSERLFYTLYVLNDKFK"
     gene            12873..14087
                     /locus_tag="PKI65_04410"
     CDS             12873..14087
                     /locus_tag="PKI65_04410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB77717.1"
                     /translation="MNKIRILRILNRFNLGGPTYNACYLTKYLEGDFETLLVGGENGR
                     YEASSLEIPHSLGIEPLILPELKREIHPYWDFLAYKKIKDLIKTFKPHIIHTHASKAG
                     AIGRWAGYNTCVPIIIHTFHGHVFDAYFNNLKSNFYVLLEKELAKKTDILIALSQNQK
                     IDLTENYKIATDEKVKVIPLGLDLKKYTFDKETKRKKVRQEFNILDDIFVFSLVGRIA
                     PIKNHKLAIDAAKIAIKESNKPIKFLFVGDGELRDELIQYTKINELNTSYKEAHGNED
                     IIFTSWRNDIDAIMAASDAILLTSNNEGTPVSLIEAQASEKPIIATRVGGVADIVKDN
                     AILVEKNNVRALAEAMIKIIDGHFINIESLVKNKDFILNNYSIEILVDNIKNLYLELF
                     NSKFGKIKIYNL"
     gene            14267..16492
                     /locus_tag="PKI65_04415"
     CDS             14267..16492
                     /locus_tag="PKI65_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB77718.1"
                     /translation="MKKIFIIGLIIQFFLPIKILYSQPSPGCPNVYTTLPGSSIPIND
                     YYLCDSGNINIEAHPFNVGATDQYIVTSIPYAPPFPFDAGSPVPGNYADDKYLYAFDL
                     PFKFCFFGEEYEQVVVGTNGIITFNTDLANEYCPWSFSVSLPNSSLPTNAIMCYHDIY
                     PLTSSGSGCGYLRYGVLGTYPCRAFVLNFDQVCHFNVQNIKTTMQIVLYEGTSAIEFH
                     IQSKPVHSGWNGGRCALGIQNSDGTIAYFPPNRNTGVWTATNEAWRFTPSGTPINIFQ
                     WYLNGDPQPVNQTSLNSFIDSNATAVAIATYNVCGFTQIVVKDTVRIHVAHLDLPYDT
                     ISICQGDSITLTADTTGAFSQIQWSNGMDGNIIHFLPDTSQMIYVNAYFPDGNYCTDS
                     VYINVIDSVYLQFIVNDTPLLDGESFNICRYDTAHITVTGANSYIWSSGDTLSQISVS
                     PDNATTYTVTGNISSCADTASITIGIHPNPNPSIKAIPKEGCIPLVVSFTSKTDVPIT
                     QYLWYLSNGITSHQSSFTYKFNDVGTYDAKLVVESVDGCKDSVFVPGIVSTYPLPNVA
                     FSMSSHSIFTDEPLVIFTNESDADSYEWQYLLQNQSVIFSNDVNPSYEFTMPGNYTII
                     LTGYTDKGCMSQAMDYLEVIGIYSLYVPTAFSPHNKDGLNDYFYPYIYGLSPDPDSYS
                     IMIFDRWGKLVFYSTDINAMWDGTIDGHDAKPGVYIWKITLKYNNNIWETQHGEFLLL
                     E"
     gene            16673..17041
                     /gene="rnpB"
                     /locus_tag="PKI65_04420"
     ncRNA           16673..17041
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKI65_04420"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17126..19036)
                     /locus_tag="PKI65_04425"
     CDS             complement(17126..19036)
                     /locus_tag="PKI65_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOB77719.1"
                     /translation="MKKIIFILSFLFGFTSLYSQKLSSNNSKAIKNFNEAIKLYENYN
                     YLDAINKFKKAINIDPLFIEAYILTAQVYSDLNMFDSSIVYYKKTVEIDQDFFPNAYM
                     FIAKFSYMTGKYEEAVKYQELYYNHYKLNPTQRARAKIDLNIFYDALELYKNPVPFKP
                     INLGPNINSIYSEYSPALTIDDSTIIFTRKVPVKDKAMEQEDFYQSKKLPNGNWELAM
                     PLLGELNTPNNEGAHTISADGKILYFTACNRSDGQGSCDIYRSYKIGNTWTKAENVKE
                     LNSVSWDSQPTLSADGKTIYFASARNGNMDIYVAHMQKNAKWSHPQPLPDNINTARSE
                     LTPFIHPDGKTLFFASDGHGGMGGYDIFYTKKINDTLWTTPQNIGYPINTHKDDAFLF
                     VNASGKYAFFASEIENGYGAYDIYMFEFPKNIITTPVTYFKGIVVDIDDNKPIIHAIA
                     ELFSLKDASIAGKAITDIDGSFLLPLPAGEIYALNISAKNYLFYSEHIELIEAYDSLD
                     PFVKYIYLRPIKKDQIMVLNNILFDFDQATLKPESYVELEKLYNFLVDNPNLVIEIGG
                     HTDSIGTKDYNLILSEKRAKAVYDYLISRNINPQRLRYKGYGDTMPIADNSTEEGRKL
                     NRRTEIKILYNN"
     gene            complement(19107..20423)
                     /locus_tag="PKI65_04430"
     CDS             complement(19107..20423)
                     /locus_tag="PKI65_04430"
                     /inference="COORDINATES: protein motif:HMM:NF003712.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HOB77720.1"
                     /translation="MKENEMDNPNIEKEKSLPSYHRGHLPPLSEDYPTFESCNDLLFF
                     HKYNPFLDKISSLETLEDLLKRDEQREKDGFHRRIRLGKLIKPLGGDKNKVIVVPTTT
                     EPKFYHDESMMPEETGGSGEGEEGQVIGKQRVRPTEGEGAGQGAGQGEGAEHEVIANA
                     FNLGKILTEKFQLPNIKQKGKKRSLTKFNYELTDINRRQGQLVEKKSTLRRIIKTNLL
                     LGNIKLGEPVDTSKLLVTNDDLVYRLVSKERDYETQAVVFFVRDYSGSMEGKPTEAVT
                     SQHLMIYSWLVYQYQYRVETRFVVHDTEAREVSNFTNYFQLQVAGGTRIAPAFQKVYE
                     IIQRDQLAKDYNIYVFYGTDGDDWDTDGKELNDNLIRLFPISARIGFTIAKNSWSINQ
                     ETTLEKNLKNYQIAKKYPKEFRVDSFDADIVDENRIIESIKVLVSE"
     gene            complement(20430..22475)
                     /locus_tag="PKI65_04435"
     CDS             complement(20430..22475)
                     /locus_tag="PKI65_04435"
                     /inference="COORDINATES: protein motif:HMM:NF018504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77721.1"
                     /translation="MTLQEHISKVASGEHKFENVFQSISRMIFGDPSKIEKVIVNGTP
                     TYDFKIFREGKKHIVGMYEEINSFASFVKDAAEGGSSAEMAFVLIGEPGNGKTFFVDF
                     LSSKYREFISKPGNQRFTFKFTNVELLDRYGQITEIESQTFEDPMILAMNIYDDIDKN
                     EAFMKENGLNNKQIKEIYKYYRPLGACTYYIWNQIKDFCNNDIKKALDFVTIFPVPVS
                     ESRGILAGKYAAKDKITSSSVDLIGDESIARLLHLKDADNPFRFDLRRGALARVAGGG
                     IHFADEIFKNKRDLIQIYLGVIQNRTIEIEGYKWPLDTLIIATSNNSEFANFLEEKEQ
                     APIVDRCRITYMSHNTNLHLQEELTTYALGSVDKTTIFGEKLHVDPNLNYVLSVAFVL
                     SRMPQHEKLTPIEMMKLAAGDVAGEKSIRTLAEVINELNTDPDITKRFGQKGLGHRSL
                     GRSIQILRERSETQEGKCMFAGDAFPSIQRVILDYVQDANDRIKYFNDLKIAKQLYRE
                     RVNTAIFNAYMNEPHAVEKDVMNYVNMIIGLESKNLGEDKTWTYVDPQTKKLVSIKID
                     ESFINSVEERLGLKTEEQKQSFRTTIIKIYGQKVLTDPNYNFMDNNELVKAVTDVRLK
                     SDIGGKGSLIGALANRTNADNQRLYDRIMDTMLNKLGYCLTCAMKTIEYFCTYDDAN"
     gene            complement(22620..23756)
                     /locus_tag="PKI65_04440"
     CDS             complement(22620..23756)
                     /locus_tag="PKI65_04440"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOB77722.1"
                     /translation="MKTIKPLIISIFIFIIGCSPYKIYNKYEDLKTNNFIEFYLPINI
                     LKIDIEIEKTDFIPGELNEYTIEFLGNIPCIQDSSTHYQIKNVKVNVFALPDTNAIYY
                     LVFNKVPKKDNFLIHFYDNGLININNENNTSYKQEKYLIDNEHCKINETQNPFNTFIS
                     KQLQEKADTIRQMIMVDSVKRIKTSIQTRLEPKTDYDLAKEISEDIYLLRSYYYDLIS
                     GIPEIGYENVTFTIMLNEIKEVYNAYLSYFTGKIIKSPINHSLKVIPSNNPDNEKIFI
                     FSFNKQSGFSYEPSKVNKDNYYLEIIPLKENPSIDNKKINNKSFPARKALPCEIRIIN
                     ETGNDIIYKEISKIAQKGYVYYLPFDTKKIIFSEINGEVLLIEL"
     gene            23860..24969
                     /locus_tag="PKI65_04445"
     CDS             23860..24969
                     /locus_tag="PKI65_04445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB77723.1"
                     /translation="MKKITISVINDLATDQRVYRTCSLLIEMGFSITLIGRKLPNSLP
                     INRPYKTKRFKFIINKGPLFYLAYNIRLFFYLLFTHQDILWANDLDTLLANSLISWIK
                     NKPLIYDSHELFTETAEVYNRKLVKKIWEVVEKIGIRHVDTFITVNESIANIYKNKYN
                     KPIYVVRNLPIKMKNHVYKPLKLKKNDGKYLIIMQGSGINIDRGYEEAVLAMKYIDNA
                     ILYIIGSGDVIDQLKSLAIKENLTDKVVFVPKLPYLEMMKYTTSADLGLSLDKPICLN
                     YLYSLPNKLFDYIQANIPIIASDLPEIRKIIEKYEIGIVIDNIDPKKIAETIKRILKD
                     KEVYEKMKENLKIAADELVWEKEKEILKIVLQSYM"
     gene            complement(25131..25313)
                     /locus_tag="PKI65_04450"
     CDS             complement(25131..25313)
                     /locus_tag="PKI65_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77724.1"
                     /translation="MRRFRGVVTKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
BASE COUNT         9184 a         3732 c         3911 g         9206 t
ORIGIN      
        1 ctcctgctac tagtttatta gtttataata cagctactac tggagatgtg acgcctggtt
       61 attattactg gaatggtaac caatgggttc gtttcgctac tggtaatatc tcaggattaa
      121 ttaattgctc atctaccaat tatcttatta aatcagatgg ggtaaatgga gtatgtactg
      181 acatcataga aaatgctaat aaaaatattg gtattggtgc tacaccaaat gataattata
      241 aaatgtatgt ttacagaccc agcactagct atggtgctgg taaagctggt atttatagct
      301 atcgatatgg gacaagtggt agtactaatg gcggtacttc ttggtctaat actggtatag
      361 atgctgctat taaagcatat tcttattggg gcaatgaata tactgctgca attgctggat
      421 atagttattt agattatact aatagtgctg ctattgttgg tgctaatagt gggggtacaa
      481 tacgagctga actagctact aaaaccccta cctatgatgg atctacatca gatggaactt
      541 attcattgaa actaactggt gatttttata atcaagatat aaactatagt caatctacag
      601 aggtatactt tactggcaca acacttcgct cagtaaatgt aacgacgcat ggcactggcg
      661 ttaataatag tatagtgtgg atacatggag aatgcgatta tactaaagac gatgcaactt
      721 cctttgtttg tttctatatt tatagagata atactttact atgcgaagtc agtgaagtct
      781 catatgtaga tgaagataaa actgtccacg tccaatggat agatgaacca ccggctggac
      841 ctcatactta tgaactacga gtatactacc ctaatggtgg aatgacatat tatggtcatc
      901 aattacatgt agtagaatta aaaagataaa aatatgaaaa aattattttt agttaatgca
      961 attttatttg tatgtttaat attaaacgga caaaataatt tgaatgatga tgctaaagct
     1021 agaatcggca cctatctaaa aattaatcca caaattttaa atgtacaaat tagctcttta
     1081 gaagctaaat ctacagtaga tattatagtt tttgcttttg agttatattt catcattcct
     1141 acagtatcac ttaataatat taaggatctt caagaaaaga aagaaataga actgctaata
     1201 agtagattag ataatttgct aaaaaatcca cctgaatggg aacatatcat tgctctacaa
     1261 aatgagaaga aatctaaacc tcccactcca cctgtttatg ctggcgatga aaaagctaaa
     1321 caatactaca atactaaata tagtgactta aataaaaaat catcaggttc taaaggcatg
     1381 ttgatacaaa atgaaactat tatagagcct cctgtaccca tacctgataa aagtaaggaa
     1441 ctgattaatg aataatttag tttgatattt ttttttaatt tataaaaaaa ttgattgtaa
     1501 taagcactca attataacta aaagtaaaaa aatactttat actttttttt taaaaacaat
     1561 ttgtcctagc tttgctattg ctagagttag aaaactattt cattgatttt tactatttca
     1621 ttaagcagta attttttaag tatattaaat tattttaaat aaattttttt ataagaaaaa
     1681 aaattattac ttttgcaaac taaaaaactt aaaaaaataa tcaatggata cattaagtta
     1741 taaaacggtg catttgaatg ctgaaactgt taaaaaagaa tggttagtgg tagatgcagc
     1801 tggtcagcct ttaggacgtt tagcttcgag ggtagcttat gttttgcgtg gcaaacataa
     1861 gccaggttat acacctcacg tagattgtgg agataatgta atcgtaataa atgctgaaaa
     1921 agtagtttta actggtaaaa aaatgtctca gaaaatgtat tatcattata caggatatcc
     1981 tagtggacaa cgtagtgaag ccgctaaaga tattttaaaa cgtaaaccta cttttttagt
     2041 agaacatgct gtgaaaggca tgctgccaaa aaatcgatta ggtagaaaaa ttttgaaaaa
     2101 tttatatgtt tatgctggac cagagcatct ccatcaagct caaaatccaa aaattattga
     2161 tattaacacg ataaaatagt acaacatgga taacaagatg atttctggta gacgaaaaga
     2221 agctattgcg aggatacgtc ttttaaaagt agaagatggt aaacaagaaa ttacaataaa
     2281 taatagggat tataaagaat atttcccatc tatatcctta cattatattg ttgaacagcc
     2341 ttttaaattg acagaaacct ggggacaata cagtgtaaaa ggaagactta atggcggtgg
     2401 caaaaaagga caggctgaag ctttacgtct agctatttct aaagcattag tagaggaaaa
     2461 tccagaattt agacaaatat taaaaaaata taagctgctt actcgtgatc cacgcatggt
     2521 agagcgtaaa aaacctggtc aacctaaagc acgtaaaaag aatcaatttt caaagcgtta
     2581 atactaatga caaatcaagg tttagtatcc aaatcattga gatcacaatt attttgtgct
     2641 actcagtggt tgcttttaat ggaaggtaaa ctaaaaaaat ataataaatg gaacaattaa
     2701 catttgatca acttttagaa gctggatgtc attttgggca tcttacgcgt aaatggcacc
     2761 ccaatatggc tccatatatt tttatggaaa aaaatgggat acatattata gatcttaata
     2821 aaacattagc aaaattggag gaagccaaac aagcagctta caatcttgca cgtgctggta
     2881 aaaagatttt gtttgtaggt actaaaaagc aagctaaaga tattgtagct aatgcagcta
     2941 aaagtataaa tatgccttat ataactgaac gatggccagg aggtttgcta acaaactttg
     3001 ttactattag aaaatctatt aaaaaaatgc agcaaatcga tagaatgatg aatgatccaa
     3061 gtttttctaa tatttctaaa cgtgaaagat tgcaattggc taggcaaaga gctaaattag
     3121 aaaaaacctt gggatctatt gctaatatgg ttcgcctacc tagtgctatt tttgtggttg
     3181 atatagtaaa agaacatata gctgtttccg aagctcgtaa actaaatata cctatatttg
     3241 caattgtaga tacaaatgct aatcctcaat tagtggatta tcctattcct gcgaatgatg
     3301 atgcagctaa atctattcaa actattttag aaaatttcgt agaagctata aaaaaaggac
     3361 agcaagagag aataggagct atggaagaag ccgaacgtga agacgaagaa tatagcgaag
     3421 aaaagttaaa agaaaagaaa ataaaggtta tggaagcttc tgctgatgca gaagatgatg
     3481 aaaagagtag taaaaaacat agaacaagaa aaaaggaata attttatggc aaatattagt
     3541 gcaaaagatg tgtataaact ccgtgatcta acaggattag gtgtaatgga ttgcaaaaaa
     3601 gctttagaag aagctgaggg tgattttgat aaagctattg agattttaag aaaaaaaggg
     3661 caaaaagtag ctagtagtag agctgataga gctgctaatg aaggtatagt gcttactaaa
     3721 attgcaaaag ataaaacttt tggtatcatt ttgatgctta attgtgagac tgattttgtt
     3781 tctaaaaatg aggaatttat taatttagct aataatattg ctgatatagc tttacaaaat
     3841 aaaattaaat caaaagaaga tttactaagt gcaaaattta atggtataac agtagctcaa
     3901 aatattaatg attatatggg taaatgtgga gaaaaaatcg aattatctaa ttatgaattt
     3961 atagaagctc cattagtact tggatataat catcataacg gacgagtagc cgctctagta
     4021 ggcttatcta aagcttctga aaaaatagaa gaaatgggac ataatctagc tatgcaaata
     4081 gctgctatga accctatcgc tattgacaaa actgacatct ctcaagatgt aatagacaaa
     4141 gaattagatg tagctagaga attagcaaga aaagaaggta aaaatgaaga tttaattgaa
     4201 aaaatagctc aaggcaaatt aaataaattt taccaagaaa atacccttct aaatcaagag
     4261 tatataatgg aaaatgataa aaatgtaatt gattatatta aatctatcga tccagagaca
     4321 aaagttatca aattttatag atttgctctg ggttcttaaa taaataatga tttttttcat
     4381 aattttattt tttgcaaaaa gcctcattat ggggcttttt gtttttaaat caatttattt
     4441 attaaattta aaaaattatt ttactaaaat aaaagtatat ttgatgtaga cgttaacata
     4501 atatacctag attttattat attttgtctg tttgagagaa tgtgagcaat ttaactatac
     4561 ttgaatatat gactacataa aaaaacttaa aatgattaaa acttctgata ttcaaacaga
     4621 tatatataga agttattttt attgtttaga attttttgat ttttctatta attgtaatat
     4681 tcattaatca gagataatat tatatatttg taaaaaaatc tgttcgattt tatttactga
     4741 tatgaaaata tatgctatta cacttttttc aaataaagaa agatataatc atataaattc
     4801 gcaccttaaa aaattacact tagattataa atttattgaa gccattgata aaaaagatat
     4861 atcagatgat gatatgaaac tatattataa tatttctaat attaatgaat taccagattt
     4921 tattaataaa ggaaatacag ctaataaaat ctcaaagctc agagcttaca aagatttttt
     4981 atcatctaat gaaaaagttg cttttttcat cgaagatgat ataatattac cacataatat
     5041 taaagaaatt ttagataaaa tagaaaaaga gataaatgat aatgaagtaa ttttgctaga
     5101 ttacagaact gtaaatacat cttataaaat aggaattagt agggcaaata gtaaaaaaat
     5161 aaataatgga ttattagcat accccacaca attattagga ttggttggga gtggggctta
     5221 tatacttcat aaaaatgttg ccgaaaatat aatcaaatta gatttatata agaataattt
     5281 ttctgactct tggaatgatt tttatcaaaa agatgctttt gatcaattga gagtattata
     5341 tcctgtagtt actaaattca aaccttttag ctctgctatt ggttatatac ctagtggtag
     5401 cttaaaatat aaaataaaaa atttcattga aagatataaa ataccattcc tttatcaatt
     5461 actcattttt aaacgcaaat atttcctacg ctcagtagct aaacaatttt tcttaactga
     5521 tgaaatctct ccaattgata aaattaaata aattgattat tctattaaaa agctaaactc
     5581 aaaaattgtt tttataaaat ataaattttg gaagtatatt aacattataa ttgacatttt
     5641 aataattata acctaaattt tatttatttt tgtagaaaaa aatcaatttt ttcagtaagt
     5701 gaggcattct tttttttatg ttttattatt tataagcttc gtaggatttt ctcaaaatgc
     5761 tattatttat aaaagttttg ataattacag ttttttaaat actaacgata gagatagtac
     5821 aaaatattta attacaccat attttagtgg acttgctagc gttgataatt atgaagtaat
     5881 gattggagca catattaaaa aaagatttca aaacaaaaat actaatttta tggtttggac
     5941 taaaccagag tttttatatt tttctttatt tgattattta aatgattatg attctatttc
     6001 tcgatttcct ggtggagaaa agacctttaa taatcaatat tcatggaacg cacccataga
     6061 actaacaatg aatgtaaaaa atcattatat tttgtctgca ggttatagca aatttcactg
     6121 gggcgatgga tataggagct tgaatttatc atacagcagt gcaaattatc catatttatt
     6181 acaacaattt aaaatcaaag gattagaatt ttcctgggtt atgatgagat ggactaatcc
     6241 attaaataaa ccgaatttcg ctaaatattc catatttcat agtttttcat ttaatattgg
     6301 cccaaaatgg cgtttcgctt tttatgaaaa tattatttat ccagctagag acagtaatca
     6361 tcaccattta gaatatgttt acttattacc acatattttt tatagatatg ttgattattc
     6421 acttgggtca ccttataatt tattatttgg tggtaatttt aattggaatg ttgggaaaaa
     6481 attccagatt tatgggcaag ccactttaga tgatttcata tttagtgaat ttagaaagga
     6541 tataaagcat ctgatacatc ccactgatag tagtatagta tatggtaatt ggatgaacaa
     6601 atatgctgtg caattaggta ttaaatggaa aaataaaaat aataaaatac gtactagatt
     6661 tgaattcaat gctgtgcgtc cctatacata tagtcacaaa aatcctattc aaaatttttc
     6721 taataatggt caggcagtag cacacccttt cggagctaat ttctatgaag cattgttaga
     6781 agcttcgttt atcaaacata attggcatat ttatctaata agtttttata agcatattgg
     6841 ctatgatact acaggcactc attttgggca aaatattttt tatcccactt ttgatgctta
     6901 tatgggcaat aacattcctg taaatccttt ttataacgtg ataggacaag gatgtctgga
     6961 gagattaatt gccgctcaaa ttaattttat ttataaagtt aaaattgact atgtgaatca
     7021 tatgtacttt ggtgtaggtt tcgaatatag atattctatt aatagagatc atttagattt
     7081 tttaccattt ataaaattgc aatttaatac agacaattta cacgtatggc atacttataa
     7141 atgagataaa taagaaaaag tataaagtga acgggggata atgtgtaagt aaatggaaac
     7201 aaataagcaa ttaaatttta atgtttaagt ttttcaagtt tatatgttaa ataattttta
     7261 tatttttgca aaaattttag gctatgaaaa aaattgtaga aaataccagt aaataccttg
     7321 ctatgagtaa ggaagagaat aaagaattct tagacaacct ttattcatta atgaaaaaat
     7381 tagaagctga tgtagtcgat tatcaaggtc tgaaaatact cagcgccccg ccactgtgcc
     7441 cagactgtcg tagcaatcac attattaaaa atggtttaca aaatgggatg caaaactatc
     7501 gctgcaaaga ctgcggcaga caatttagag ttaccactgg tacttttgtt tacaggttac
     7561 aaaagaaaga gaaaatgctc gactacatca gatgtatggt agcaggcaaa agtctcagag
     7621 cttgtgctaa agaagtgggc atcagcctac caacaagctt cgcttggcga cacaagatat
     7681 tggcagctct acaaagtttc gaagataata tcaatttttt cggagtagta gagatggatg
     7741 aactattgat ggattattct gaaaaaggca gaatatacaa aaatgccgaa gagctgaagc
     7801 gagctaggaa gcaaaagcca aagaaagtag ccgtattggc tgctacagac agagctggca
     7861 acttattatt caaaaaattt gatggcaaaa agctaagttc agaacagata gagcaatttc
     7921 tgaaggctaa aatgccaaag gatggagtca tgtgtagcag taataaaaaa gcattcaaaa
     7981 aggttagaaa aaatgtaatc aagcacaaag aaatagtaac tagcaatagg caaaagcgag
     8041 gtatatatag cctaagtact gtcaagaaga aagctagtga attcacgaat tggatatact
     8101 tcaaatttag gggcgtagct accaaatacc tgcaaaacta cattatgtgg tatgtagtga
     8161 tgggtaaata cctcacaggc aagactgtaa gcaatactgg acgaatgctc aatctcgcat
     8221 cttcggacag gtgggcgtgg tatcggtata gggagctaat aaatataaca tatgttaatt
     8281 aaaacatatg ttagttttcc aaattcatac aataccactc caataatttc tatcaataat
     8341 attaattaat attactagaa tatataaata attttctcag cctctaaaac tttaacttac
     8401 cacataaaag atttatctca atacctattt aaaatatata aaataattat taaattaggt
     8461 gtttaatcta taattaaatc attgttcata tcatatttat tgttttatat atgttaatgc
     8521 tatacactct ataatagttt tgtattctct gttttttgga tttaagcctg ttgtttctct
     8581 ttccttacaa ccatgacctt taggtcaggg ttaatatcta gaagattgcc tataattaat
     8641 cacaactttt agtcatggtc ttaattattc ggactttagc cctgaaaaag ggtatatctt
     8701 tttctttgtt catctcctga gctaaagctc gaggtgatta tgtgcataaa attatatacc
     8761 tatacgatga tttattagtg ggtattcata tattgaccgt gcaaaacccc gttttttata
     8821 atcttaatgt ctattaaaaa aattatttat aaatctacaa caatctaaat aaaaatgcct
     8881 ctaattaaat ataatgtagc aaaaatttgt gtatagttaa tgatatacaa ttaaaaagta
     8941 ttttaacaaa tttattatat aattttcacc tctggtgtca acatgatacc aaactgttcg
     9001 tttacagaat taataatttt atttgcaagg attaaaatat cttttccttt tgcattacca
     9061 taatttacta ggatgagaga gtgagttgat gatacaccag catcaccctc tctatagcct
     9121 ttccagcctg ctttttcgat taaccaacct gctgatattt tatatttatc attattataa
     9181 ggataatagg aaatatcagg atatttagat cttaaatctg aaattttatc ataactacat
     9241 attggatttt taaaaaatga tcctgcattt cctattttat ctggtgaggg aattttttta
     9301 tctcttatat ctaaaatagc ttgtcttatt tcagttattg aaggattata attttgaaat
     9361 ttatttttta aatcattata agaaagatta ggattagctc gttttttaag ttttatttct
     9421 atcttagtaa taaaaaattc tggcatttgc tgtaataaac tttttctata gccaaaatct
     9481 aatttatcag aagaataaat ttcgaaatca cattttttaa aattaaatcc ataaactttt
     9541 tgtaaaactg aagaaaattc agctccgtaa gcaccaatat tttgtatagg agctacagca
     9601 cagctagatg gtatgccaga tagattttct accccccaat attcattttc tacacaatat
     9661 gacacaagct tatcccattg agtaccacct cccgcagtta tcacaatatt atccttatct
     9721 tcattgatga tttttataaa atctataacg gaatgtatta caaatgatat agtatctttt
     9781 gtgaataaca tattactacc ttctcctaaa aaaacagctt ttttatatat atctggatat
     9841 tgtttattta gttttattag atcatcacta tcttctattt ctatgaaaat atctgcataa
     9901 cactcgcaat gaaaagtgtg tttgtctttt aatgataaat ttcgtaaaat attcatttat
     9961 tattggcatt acattattct gaaataaaaa aataataaat atttattttt gtttatattc
    10021 ttcatagttt tctggtaaaa aatcaaagaa agtgtcgccg cgtagcccga gacgaagtgt
    10081 ttctagtgct attacatcct gatatgctac attacccaaa ttcacattag tgccaaactc
    10141 ttttataaaa tatacttgtt ggtttttttg gggtgcttca aatatgattt tatcgacagg
    10201 taattctttt ttgatttttt cgattaacat cacacgtgca ttacctgatt tagtatatat
    10261 acccacatta ccagattcac gtgcttcgcc tattacatat gtagaacctg catctaattc
    10321 atctttcatt tcttttaccc atagatttgg ccttactatt atagattcat ctttactccc
    10381 cacttcagac aatacaagat aatctttagc taaaatgcta atgtattcac attttttttc
    10441 atgttctaaa gctatacttc catcagaaac ctctacagtg tcaatcttat attttttaac
    10501 ccatttaata aaatcagaaa attgctttct aataacaaaa gcttcgaata gagtgccgcc
    10561 aaagtatact ttcaccccag cttctttgta tatttgaatt tttttctcaa gattttgagt
    10621 aataatagaa gtgccaaagc ctagtttgac aaaatctatc aaatctgaag caacttccat
    10681 gagcccttcg gcttcgatag gtgataagcc tttatccatt accatcgtaa tacctgtttt
    10741 gcgtatcttt tttgtgcgtt cgggtaaatg ttttaatatc ataatctgaa tttttttgct
    10801 aaaatatgat ttttttaata aataaatatt attataaaaa atgcaatatt tcatttttta
    10861 tttatatata aatatataaa aataaggtag attagcatta attttattgt ttgtttgaaa
    10921 tattttctgt gatttactct attatacata caatattcaa aattataata aatctttaaa
    10981 tgatattatt ctttattttt gtacttttat ttgtattatt tattaatgtc tagtattgca
    11041 ggtatttact ctataaataa agatgatttt attttatcta tcaataaatc atctgttata
    11101 tttgtaaatg ataggtgtga taggcaaaat tatgaatttg taaattctaa actaatactt
    11161 atagattcta aaacttctaa ctataatcaa tctgttaatc agcgtatctg ggaccatacc
    11221 aatagatatg ttttgatttt tgatggagaa atttttaatc acttagaatt aaaaaataag
    11281 ttaattaatt taggatataa tttagctact aattcaacac ttgagataat tttatacttc
    11341 ctaatacatt atgatgttga agcattagaa tcgttcaatg gggtttttgc tcttgctttt
    11401 tatgatacag tagaggataa attgatttta gcaagagata gatttggtag taaacagctt
    11461 tattattact tcgataaaaa ttctttttat tttgcttcag atatcaaaac cctaagatat
    11521 ataaatttac cgctcaattg ggattattct tctctaacac tttatttttt tcttacttat
    11581 ataccaagtc ctttcagtgc tttcgaaggg gtaaataaac ttttacctgc acactatcta
    11641 atctataatg gtagagatat aatgataaaa aaatattatg agcttccaga acaaatcgat
    11701 gatgtcaatg aaatcaaaga atgcatagat tctatagatt ttttattaaa acaaagtatt
    11761 gagcaacgtt tgaatatcga tttgccgata gggacatttt tgagtggagg cattgattct
    11821 tctattatta cttatttagc acatgagttt aggccagata tcacagcata tactattagt
    11881 tttcccgatt taccatatta tgatgagtct gatattgcta aacaatttgc tcttgataat
    11941 aatatttctc accagatcat tccaattgta gaaaatgatt taatcaattc aattagcaac
    12001 gttttatatt gcatggatga accttttgca gatagttcta ttatagctct atattcactt
    12061 gtggaagcta tcaaacccca ggctgagata atgcttactg gtgatggtgc agatgaacta
    12121 tttggaggat ataataaata tagagcatgg gccagattac ataagcgaaa ttttttgaat
    12181 agtttaatta tttctttagt gaatacgata ccttttcata tttatgatac aagtatttat
    12241 ttatgtaata aattaagaca gttgcaaagg ttttcaaaag cttatcaatt gactgatttt
    12301 gatagatatt tctacctcag ctctatgaat gatgtatttt ttgtacaaaa aatactcaat
    12361 cctgaactat taaatgataa atctttcaaa gaaaaaattg attttttgaa aaatatttat
    12421 tacaacgaaa ataaaaacat tttgtctata gattttaaat ttgtcctcga aggtgatatg
    12481 ttacgaaaaa tagattttgt aagctcggat aataatgtat tattgcgttc tccttttctt
    12541 gattatagat tggtagataa tgttttcaaa atacctgaat cctataagtt taatgctaaa
    12601 acaaataaaa ttttacttag agatttatat aaaaatattt taccagatta tataattaat
    12661 agacccaaac atggcttcga ggtacctatt agaaaatgga tgaatgattt tttattagat
    12721 gatttgatta aaaaaggatt aaatgtaaat ttaaaaccat tattaaatga taatgtaata
    12781 aaaaatcaaa ataaattata taaatttgca aacaaatcag aaagattatt ctacacatta
    12841 tatgttttaa atgataaatt taagtaataa ttatgaataa aataagaatt cttcgaatat
    12901 tgaatagatt taatctaggc ggtcccactt ataatgcttg ttatttgact aagtatttag
    12961 aaggagactt tgagactctt ttagtaggag gcgaaaatgg tagatacgaa gcatcgtcat
    13021 tagagatacc tcattctctc ggtatagagc ctttgatttt acctgagttg aaaagggaaa
    13081 tacatccata ttgggatttt ttagcataca aaaaaataaa agatttaata aaaaccttta
    13141 aacctcatat aatacatact catgcatcta aagctggtgc tataggtaga tgggctggat
    13201 acaatacttg cgtgcctatc atcatacata cttttcatgg tcatgtgttt gatgcatatt
    13261 ttaataattt gaaatctaat ttttatgttt tattagaaaa agaattagct aaaaaaacag
    13321 atatattgat agctcttagt caaaatcaaa aaattgattt aactgaaaat tataaaatag
    13381 ctactgatga aaaggtaaaa gtaatacctt taggtttgga tctcaaaaaa tatacttttg
    13441 ataaagaaac caagagaaaa aaggtaaggc aagaatttaa tattttggat gatatttttg
    13501 tatttagtct cgtaggcagg atagccccta taaaaaatca taaattagca atagatgctg
    13561 ctaaaatagc tattaaagaa tccaacaaac ctattaaatt tttatttgta ggtgatggag
    13621 agttgaggga cgaactgata caatacacaa aaataaacga attaaacact tcttataaag
    13681 aagctcatgg caatgaagat atcattttca ctagttggcg aaatgatata gatgctataa
    13741 tggcagcatc agatgctatt ttattaacct ctaataatga aggcacgcct gttagcctaa
    13801 tagaagctca agctagtgaa aaacctatta ttgctactcg tgtaggtggt gtagctgata
    13861 tagtaaaaga caatgctata ttagtggaaa aaaacaacgt cagagcattg gctgaggcta
    13921 tgataaaaat aatagatggc cattttataa atattgaatc cttagtaaaa aataaagatt
    13981 ttattttaaa caattattct atcgaaatac tagtggataa tataaaaaat ctttatttag
    14041 agttattcaa tagtaaattt ggaaaaatta aaatctataa tctgtaaacc cacaactaat
    14101 aacttatctt tgtaattttg ttttgatatt cttctttttg ctatattatt ctcttgatgt
    14161 tgcatttatt aattgaattt agggaaattt ttttaaaaaa aaaatttttt gtagttttgc
    14221 aataataaac ttttgtaaat aatttttgtc taattaataa aattatatga aaaagatatt
    14281 cataattgga ttaattatcc agtttttttt accaattaaa attttatact ctcagccaag
    14341 tcctggttgc cctaatgtat atactacatt acctggttca tcaattccaa taaacgatta
    14401 ttatctatgt gactctggaa acataaacat agaagcacat cccttcaacg tgggtgctac
    14461 agatcaatat attgttacta gcattcctta tgctccacct ttcccatttg atgctggatc
    14521 acctgtccct ggtaattatg ctgatgataa atatctatat gcattcgatt taccatttaa
    14581 attttgtttt tttggtgagg aatatgagca agtagtagta ggcacaaatg gcatcattac
    14641 atttaataca gatttagcta atgaatattg tccatggagt ttttcagtat ctttacctaa
    14701 ttcgtctttg cctactaacg ctattatgtg ctaccatgat atctatccat tgacgagttc
    14761 aggaagtgga tgtggctatc tgagatatgg agttttaggc acatatccat gtagagcttt
    14821 tgttttaaat tttgatcaag tttgccattt taatgtgcaa aatataaaaa ctactatgca
    14881 gatagtgctt tatgaaggca ctagtgctat agaatttcat atacaaagca aacctgtgca
    14941 ttctggctgg aatggtggta ggtgtgcatt aggtatacaa aattctgatg gtactatagc
    15001 atattttcca ccaaacagaa atactggtgt gtggactgca acaaatgaag cttggcgttt
    15061 cactccatca ggtactccaa ttaatatatt tcaatggtat ctcaatggtg atccacaacc
    15121 ggtgaatcaa acatctctta atagtttcat tgattctaat gcaactgctg tagctatagc
    15181 cacttataat gtttgcggat ttactcaaat tgttgttaaa gatactgtgc gtatccatgt
    15241 tgctcacctg gatttacctt atgatacaat tagtatttgt caaggtgatt ctatcacact
    15301 tacagcagat actactggtg ctttttcgca aattcaatgg tctaatggaa tggatggcaa
    15361 tattattcac tttttacctg atacatctca gatgatttac gttaatgcat atttcccaga
    15421 tggcaactat tgcacagatt ctgtctacat taatgtgatt gattctgttt atttgcaatt
    15481 tatagttaat gataccccac tccttgatgg tgaatcattt aatatttgca ggtatgatac
    15541 tgctcatatc actgttactg gcgctaattc ttatatttgg tctagtggag atactttatc
    15601 acagatttct gtctctccgg ataacgcaac tacttataca gttactggca atatatctag
    15661 ttgtgctgat acagctagta taactatagg tattcatcca aatccaaatc cttcaataaa
    15721 agctatacct aaagaaggat gtattccttt ggtagtatca ttcacttcaa agacagatgt
    15781 acctattact caatatctat ggtatttaag taatggtata acttcacatc agagtagttt
    15841 tacatataaa tttaatgatg ttggcactta tgatgctaaa ttagtagtag aatcagtaga
    15901 tggttgtaaa gatagtgtat ttgtaccagg tattgtgtca acttatcctt taccaaatgt
    15961 agctttttct atgtcttctc atagcatttt cactgatgaa ccattagtaa tatttactaa
    16021 tgaatcagac gctgacagtt atgaatggca atacttatta caaaaccaat ctgtaatatt
    16081 ttctaatgat gttaatccat cttatgaatt tacaatgcca ggaaattata ccattattct
    16141 tacaggttat acagataaag gatgcatgtc tcaagcaatg gattatttgg aggtaatagg
    16201 catttattct ttgtatgtcc ctactgcctt ttcaccacac aataaagatg gactgaatga
    16261 ttatttctat ccatatatat atggactttc tcctgatcct gacagttatt ccattatgat
    16321 atttgataga tggggaaaat tagtgtttta ttcaacagac attaatgcta tgtgggatgg
    16381 cactattgat ggtcacgatg ccaaaccagg agtttatatt tggaaaatca cgttgaagta
    16441 taacaacaac atatgggaaa cacagcatgg tgaattccta ttgttagaat gatcaaaatc
    16501 atttaataaa ataattaatg ttactacaaa agtactaact tgaagacata taatatatat
    16561 tacatatgca ctaaagaatt taatatactt tactcaatta ttttcaaaat gtgtagcaat
    16621 attgtgaatt aattctagtt tatctcaaaa aaattttgta attttgcatt aaaaggtatt
    16681 gtgtcttatt gctctttgaa aaaaagagag gaaagtccag acaccgaaga ccgccatgct
    16741 tccgagatgg aaggatcttt gttagagata acaaaggtac agatagtgtc acagaaaata
    16801 taccaccctt aataaaaggg taagggtgaa aatgtgaggt aagagctcac acatctattt
    16861 ggtaacaagt agatgcgata aacctcatgg ggtgcaagac catgtatacc agcgcttgag
    16921 acttggtcgg tcgagctgga gggtaggtcg cttatgtata ttggagacag tatactcaga
    16981 taaataataa ggctatacta taatgtatag acaaaatctg gcttatagca ataccttttt
    17041 tttgtatttc aaataataaa ttctatgtgt ttgagggtat aaaatcaaaa gtataagatg
    17101 tagaatttta gaaagtttta tgttattagt tattatatag tatttttatt tctgttcttc
    17161 tatttaattt gcgtccttct tctgtagaat tgtcagctat aggcattgta tctccgtagc
    17221 ctttatatct aagcctttga ggattaatat tacgagatat taaatagtca tatacggctt
    17281 ttgcacgttt ttcacttaga atcaagttgt aatctttagt acctatactg tcagtgtgcc
    17341 cccctatttc gataactaga ttaggattat ctactaaaaa attatataat ttttctaatt
    17401 ctacatatga ttctggtttt aaagtagctt gatcaaaatc aaacagtata ttattgagta
    17461 ccataatttg atctttttta ataggtctca aataaatata ttttacaaag ggatcgagag
    17521 agtcgtatgc ttctattagt tctatatgtt cactataaaa aagataattt tttgcactta
    17581 tattaagagc atagatttct ccggctggca gtggcaataa aaatgaacca tctatatcag
    17641 taatagcttt gccagcaata gaagcatcct taagagaaaa taattcagca atagcatgta
    17701 ttattggctt attatcatca atatctacta ctataccttt gaaataagta acgggagtag
    17761 tgataatatt ttttggaaat tcaaacatat agatgtcata agcaccatag ccattctcaa
    17821 tttctgaagc aaaaaaagca tacttaccac tagcatttac aaataagaaa gcatcatctt
    17881 tatgagtatt tatcggataa cctatatttt gaggggttgt ccacagagta tcatttattt
    17941 ttttggtata aaaaatatca tatccaccca taccaccatg gccatcagaa gcaaaaaaaa
    18001 gagttttacc atcgggatga atgaaaggag tcaattcaga cctggccgta tttatattat
    18061 caggtaaagg ttgaggatgg ctccacttag catttttttg catatgcgcc acatatatat
    18121 ccatattacc atttcgagct gatgcaaaat atatagtttt accatcagca gataaggtgg
    18181 gttggctatc ccaggaaaca gaatttaatt ctttaacatt ttcagcttta gtccatgtat
    18241 tccctatttt gtaacttcta taaatatcgc aactcccttg tccgtcactt ctattacaag
    18301 ctgtgaaata gagaattttg ccatcagcag atatagtatg tgcaccttca ttattcggag
    18361 tatttaattc acctagtaaa ggcatagcca actcccaatt accatttggt aattttttac
    18421 tttgataaaa atcttcctgt tccattgctt tatctttaac tggtactttt cttgtaaaaa
    18481 ttattgtgct atcatctatc gttaatgcgg gagaatattc tgaataaata gaatttatat
    18541 taggtcctaa atttattggt ttaaaaggca caggattttt atataattct aaagcatcat
    18601 agaaaatatt taggtctatt ttggctcttg ctctttgggt aggatttagt ttgtaatggt
    18661 tataatatag ctcttgatat tttacagctt cttcatattt gccagtcata tagctaaatt
    18721 tagcaataaa catgtaagca ttaggaaaaa aatcttgatc tatttctact gtttttttat
    18781 aatatacaat tgatgaatcg aacatgttca aatcactata cacctgtgct gtcaggatgt
    18841 aagcttctat aaacaatgga tcaatgttaa tagctttttt aaacttgttt atggcatcga
    18901 gatagttata attttcatat aattttatgg cttcgttaaa attttttata gcttttgaat
    18961 tattagaact taatttttgt gaataaagtg aagtaaatcc aaataagaag ctaaggataa
    19021 aaataatttt tttcatctat taaatttaag gattatataa tacacatatt gatataaatt
    19081 attacaaatc tccatacata aaaaaattac tcacttacta atactttaat agactcaatt
    19141 attctatttt catctacaat atcagcatcg aagctatcta cgcgaaattc tttaggatat
    19201 ttttttgcaa tttgataatt tttaagattt ttttcaagag tagtttcttg atttatggac
    19261 catgaatttt tagcaattgt aaatcctatc ctagcactga taggaaaaag tctaatgagg
    19321 ttatcattca gctctttacc gtctgtatcc cagtcgtcgc catctgtgcc gtaaaacaca
    19381 tagatattgt aatcttttgc tagttgatct ctctgaataa tttcataaac tttttgaaag
    19441 gcaggagcta tacgagtgcc accagccact tgcaattgga aataattagt aaaattagaa
    19501 acttctctag cttcagtatc atgcacgaca aaacgtgtct ctacacgata ttgatattga
    19561 tagacgagcc aggaataaat cattagatgt tgagaagtaa cagcttctgt aggtttgccc
    19621 tccatagatc cgctataatc gcgaacaaaa aacactacag cttgcgtttc ataatcacgt
    19681 tctttagaga ctagtcgata tactaaatca tcattagtaa ctaaaagctt agaagtatca
    19741 acaggctcgc caagtttgat atttccaagt aataaattag tttttataat cctacgcaga
    19801 gttgattttt tttctactaa ttgaccttgt ctgcgattaa tatctgtaag ttcgtaatta
    19861 aattttgtaa gtgatctttt ctttcctttt tgcttaatat taggcaattg aaatttttct
    19921 gttaatattt tacctaaatt aaaagcatta gctatgactt catgttcggc gccttcgcct
    19981 tgtccagcac cttgtcctgc accttcgcct tcggtaggtc gcactctttg tttaccaatg
    20041 acttgtccct cttcaccctc gccactacct cctgtttctt caggcatcat gctctcgtca
    20101 tgataaaatt ttggttcggt agttgtgggt actacaatta ctttgttttt atcaccacca
    20161 agaggtttta ttaattttcc caacctaata cgtctatgaa atccatcttt ttctctttgt
    20221 tcatcacgct ttaataaatc ttccaaagtt tcaagagacg aaattttgtc tagaaatgga
    20281 ttatatttat gaaaaaataa taggtcgtta caactttcaa aagtaggata atcttcagat
    20341 agaggtggca aatgtccacg atgatatgat ggcaagcttt tttctttttc tatatttgga
    20401 ttatccattt cattttcttt cataactaat taatttgcat catcataagt gcagaaatat
    20461 tcaatagttt tcatagcaca tgtaaggcaa tatccaagtt tatttaacat tgtatccatt
    20521 atcctatcat aaagtctctg attatcagca ttagtcctat tagctaaagc accgattaga
    20581 ctacctttgc caccaatatc actttttagc cttacatctg ttacagcttt tactaattca
    20641 ttattgtcca taaaattata attagggtca gttaatactt tttgcccata aatttttata
    20701 atagttgttc tgaaactttg tttttgttct tcagttttta gtcccaatct ttcctctaca
    20761 ctattaataa agctttcatc tatcttaatg ctaactaatt tttttgtttg tggatctaca
    20821 tacgtccatg ttttatcttc tccaagattt ttagattcta atccaattat catatttaca
    20881 taattcatca catctttttc tacggcgtgt ggttcattca tataagcgtt aaaaatagca
    20941 gtattcacgc gttcacgata caattgttta gctattttca aatcattgaa atattttatt
    21001 ctatcatttg catcctgcac ataatctaag ataactcttt gtatagaagg gaatgcatct
    21061 ccagcaaaca tacatttacc ttcttgcgtt tcactacgtt ctctcagaat ttgtattgaa
    21121 cgaccaagac tgcgatgccc gagacctttc tgaccgaagc gttttgtgat atcaggatca
    21181 gtatttaact catttatcac ctctgccagt gtacgaatgc ttttttctcc agctacatcg
    21241 ccagcagcaa gcttcatcat ttctattgga gtgagttttt catgttgagg catacgagat
    21301 aggacgaaag ccactgataa tacataattg agattagggt ctacgtgtag cttttcaccg
    21361 aaaattgttg ttttatctac agaaccaagt gcataagttg tcaattcttc ctgtaaatgt
    21421 agatttgtat tatgactcat atatgttata cggcatctat ctactatagg agcttgctct
    21481 ttttcttcaa gaaaattagc aaattcagaa ttattagagg tagctattat caaagtatcc
    21541 aaaggccatt tatatccctc aatttctatt gttctatttt gtatgacgcc aagataaatc
    21601 tgaatgaggt cccttttgtt tttgaaaatt tcatctgcaa aatgtatgcc accaccagct
    21661 acacgagcta gagcacctct acgtaaatcg aaacgaaaag gattgtctgc atcttttaaa
    21721 tgtaacaatc tagcgatact ctcatcacca atgagatcta ctgaagagga agttatcttg
    21781 tctttagcag catatttacc agctaaaata cctcgagatt ctgaaactgg tactggaaat
    21841 attgttacaa aatctaaagc ttttttgata tcattattac aaaaatcttt aatttgattc
    21901 caaatataat aagtgcaggc tcctaatgga cgataatatt tatatatttc tttgatttgt
    21961 ttattattaa gtccattttc tttcatgaaa gcttcatttt tatctatatc gtcataaata
    22021 ttcatagcta aaatcatagg atcttcgaag gtttgacttt ctatttcagt gatttggcca
    22081 taacggtcta atagttctac atttgtaaat ttaaaagtaa aacgctgatt gcctggttta
    22141 ctaataaatt cgcgatattt agaactaagg aaatctacaa aaaaagtttt accattgcct
    22201 ggttcaccaa ttaggacaaa agccatctct gctgagctgc caccctcggc agcatctttt
    22261 acaaaagaag caaagctatt tatctcttca tacataccta ctatatgctt tttaccttca
    22321 cgaaatattt taaaatcgta ggtaggtgtc ccatttacta ttactttttc tattttggat
    22381 ggatctccaa agatcatcct gctaatagac tggaaaacgt tttcaaattt atgttctcca
    22441 cttgctactt tcgaaatatg ctcttgcaag gtcataaata atttttctat aaaaatatac
    22501 aatttttttg atatgacgta aaaaacaaaa ttattttttt gctttatatg atattgagaa
    22561 atttcagatc tgaaatatta gatagacact cattgaagca tatacaaaga ttttaccaat
    22621 tacaattcta tcaataaaac ctcaccattt atttcagaaa aaattatttt tttagtatca
    22681 aaaggtaaat aataaacata ccctttttga gcaatttttg atatttcttt ataaataata
    22741 tcatttccag tttcatttat tattctaatt tcacaaggta atgcttttct ggctggaaat
    22801 gatttattat ttattttttt attatcaatg cttggatttt ctttgagagg tattatttct
    22861 agataataat tatctttgtt aacttttgat ggttcatacg aaaatccact ttgtttatta
    22921 aaagagaaaa taaaaatttt ttcattatca ggattattag atggaattac ttttaaagaa
    22981 tgattaattg ggctttttat aatcttaccg gtgaagtatg ataaataagc attatatact
    23041 tcttttattt cattaagcat tatagtaaaa gtaacattct cataccctat ttcaggtatt
    23101 ccacttatca gatcatagta ataacttctg agtaagtaaa tatcttcgga tatttctttt
    23161 gctaaatcat agtcagtttt tggttctaag cgtgtttgta tactcgtttt tattctttta
    23221 accgaatcta ccattatcat ttgtctgatg gtatctgcct tttcttgtaa ttgcttcgat
    23281 ataaatgtat taaatggatt ttgtgtctca ttgattttac aatgttcatt atctattaaa
    23341 tatttttctt gtttatatga tgtattattc tcattattaa tatttataag tccattatca
    23401 taaaaatgaa ttaaaaaatt atctttttta ggaaccttat taaaaactaa ataataaata
    23461 gcattagtat ctggtaatgc aaatacattt actttcacat tttttatctg gtaatgtgta
    23521 gagctgtctt gtatgcaagg tatattacct aaaaattcta tagtatattc atttaattct
    23581 ccaggaataa aatctgtttt ttcaatttcg atatcaattt taagaatatt tataggtaaa
    23641 taaaattcaa taaaattatt agtttttaaa tcctcatatt tattataaat tttatatgga
    23701 gaacatccaa ttataaatat aaaaattgat attattaatg gttttatagt tttcataatt
    23761 aaattttttt gctaatttaa tataaatatt tcaagttttg aacaaattat ttatctttga
    23821 ttttaaatat tttattattt ttttttaatg ctaaaataaa tgaaaaaaat cacaatatca
    23881 gtaataaatg atctcgctac agatcaacga gtttatcgca cttgttcttt attgatagaa
    23941 atggggttca gtattacgct tattggtagg aaattgccaa atagtctacc gataaataga
    24001 ccatacaaaa ctaagagatt caaatttatc atcaataaag gaccgttatt ttatttagca
    24061 tataatatta gacttttttt ttatcttctt tttactcacc aagatatatt atgggctaat
    24121 gatttagata ctttactagc aaattcctta attagttgga taaaaaataa accattaatc
    24181 tacgatagtc atgagttatt tactgaaact gcagaagtat ataataggaa attggttaaa
    24241 aagatatggg aagtagtaga aaaaataggt attaggcatg tagatacctt tattactgta
    24301 aatgaatcta ttgcaaatat ttataaaaac aaatataata aaccaattta tgtagtaaga
    24361 aatttaccta ttaaaatgaa aaatcatgtg tataaaccat taaaattaaa aaaaaatgat
    24421 ggcaaatacc ttataataat gcaaggttct ggaatcaata tagatagagg ttatgaagaa
    24481 gcggtattag caatgaaata tattgacaat gcaattttgt acatcatagg ctcaggagat
    24541 gtaatagacc aactaaaatc tcttgccata aaagaaaatt taactgataa agtagttttt
    24601 gtccctaaac ttccatattt agaaatgatg aaatatacaa catcagcaga tttgggactc
    24661 agtttagata aacctatttg tttaaattat ttatatagtt tgccaaataa acttttcgat
    24721 tatattcaag ctaatatccc cattattgct tctgatttgc cagaaattag aaaaattata
    24781 gaaaaatatg aaataggcat agtaatagac aatatagacc ctaaaaaaat agcagaaacc
    24841 atcaaaagga tactaaaaga taaagaagta tatgaaaaga tgaaagaaaa cctaaaaata
    24901 gctgctgatg agcttgtatg ggaaaaagaa aaagagattc taaaaattgt tttacaaagc
    24961 tatatgtaat ggcgatagtt taagcatatt ataaatagag cttaataaag tagaaatgtg
    25021 tttggcttta gtaatgagta atattttagc tagctaaaat attacttggt ggaatcgaaa
    25081 agggaatcga aaagggtcca gaaacaaatc attgttttac ttgaagtagt ttatctatat
    25141 ttcgtattca ccagccgcca atactcatcc cacgcccatc tgtccgagga tgcgaggttt
    25201 aaaagccgac caatgtcaga ttctatctca cttgtcaagt atttatgtgt gattacaaac
    25261 cacatcaaat agctctggag gtatttggta accactcctc taaatcgtcg caaccaaacc
    25321 ttgaaatcgt ttgactactt gtacactata tacaccgcgt ctaattattt aattatgcta
    25381 taaaatacgt aaagtaaaac aaaggatatt tttatatagc tccgttcaca aatcgtattt
    25441 acaaaacgta atctttacaa agctaaattt cctggatcta tttatttaaa ttggaataga
    25501 gattatagaa aaagttctat aacgttttgt ataggtaaaa attaaaaaag agtgatttta
    25561 taaaaaattt gttaatattg gtttatttgt tataggataa tcaaatatta accacaaagt
    25621 tctcaaagaa tgcacaaaga acgcagagaa ggatggaatt aaaaaagcta caaatggcac
    25681 tttgtggact ttgtgtaata acttggtgcc ctttgtggta aaaaaatcaa cctaaataat
    25741 aaaaaaatag ttattttttg taataatcta aaattttatt acacaataaa cgttatagaa
    25801 ccaaaaaaat tattgtaatt gtaaaggaat cgaaaggtgt ccagaaacaa atcattgttt
    25861 tacttgaagt agtttatcta tactttgtat ttaccagccg ccaatactca tcccacgccc
    25921 atctgtccga ggatgcgagg tttaaaagcc gaccaatgtc tgattctatc tcacttgcca
    25981 agtatttatg tgtgattaca aaccacatca aatagctctg taagtatttg gta
//
LOCUS       DAOPRN010000026        25713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1542_length_25713_cov_175.441110, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000026 DAOPRN010000000
VERSION     DAOPRN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25713
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1542_length_25713_cov_175.441110"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1033)
                     /locus_tag="PKI65_04455"
     CDS             complement(47..1033)
                     /locus_tag="PKI65_04455"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77725.1"
                     /translation="MDNFNLLDYQALPKEQKRRFLDNLYQFLLENNYTAVDYQKLKIQ
                     STAPICPRCESENVIKAGVRDGKQVYKCKDCGRQYRETARTFVYRMRKADKMLDYLKC
                     MLEGKSLRACASEVGISLRTSFNWRHKILAAIKSLQGGISFSGIVEADELLMKYSEKG
                     RKYRSRKEYEKAKKKKHPKVAVLVMTDREGNLLFKHVGRKKVTNSQIREELSHRVTDS
                     NLICVKQKKEFKKAVKGTKSKKVVVNKTQIRRGVYSVQVVDKHIDDFKVWLRRFRGVA
                     TKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSDRWAWDEYWRLVNTKYR"
     gene            1264..2751
                     /locus_tag="PKI65_04460"
     CDS             1264..2751
                     /locus_tag="PKI65_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOB77726.1"
                     /translation="MKKSIKLKKLDLYLIKSFIGPLLVTFIIAIFVLLMQFLWSYIDE
                     LAGKGLSISVILQLMFYASATFVPMSLPLAILLSSLMTYGNLGENYEIIAAKASGISL
                     WKLMKPLAIVSILLACFTFYFSNTILPKANLKMMSLLRDVREQKPTLNIREGIFNKDL
                     DNYVIKVEKKHKDGKHVENIIIYDHSDFGPALRITTAQSGIMETTPDERYLIFTLFNG
                     MSYVELRENRDDYIRIPLQRIYFDEQVLHIDLSSFAFQRSDPSILKEHYKMLNLSQLK
                     YYIDSLDRDLKNLIENHTNMLLMTFNNFWIYVANDSNFKPMQNITVNTFDSLINLLDD
                     DSHREMYRSASEHVLNNMNMIDNQAELYRSIKKSKTYYEIEWHRKFTLSLTCLLLFFI
                     GAPLGAIIRKGGMGFPLVVSVIVFVLYYIITILFEKTAKNMLISPFLGMWLPTFILLT
                     FGLWISIKTIKDSPLMEADAWIKKINNINQYFKKLFKIKNNNKWH"
     gene            2742..3161
                     /gene="ybeY"
                     /locus_tag="PKI65_04465"
     CDS             2742..3161
                     /gene="ybeY"
                     /locus_tag="PKI65_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOB77727.1"
                     /translation="MALIFNNLNAIKSLNTNFLNKIKKIIKQILNDYEFKEGKIIINL
                     VNDNELLQINKNFLNHDYYTDIIVFNEIKNNYLFAELYISYDRLIENANKYNQSIENE
                     TARLIIHGILHSCGEDDNDEKSKQSMTIKENYYLNFY"
     gene            3234..4742
                     /gene="xseA"
                     /locus_tag="PKI65_04470"
     CDS             3234..4742
                     /gene="xseA"
                     /locus_tag="PKI65_04470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOB77728.1"
                     /translation="MENFITLKELAKQIESIINLNFNNTYWVLLEIQKLNLYRTGHAY
                     PELVEKDEKTNKIVAQMRGIIWANDYLYIQRKFIQVLGSEMKDGIRVLAKCIVNFHPV
                     YGLSLRIMDIDTTYSQGLLEQERLLTIKKLQQQGLLDANKQIPFPLVPQRIAIISIET
                     SKGFSDFVGVLANNEHCYSYKYTLFPAVLQGEKAVETITEQLKIINLKKDFYDIVVIV
                     RGGGGEIGLSAYNDYSLAKAIATFPLPVLTGIGHSTNLTVCDMVAHYSAITPTDLGYY
                     IIDKTLEFENMLLNIGDKLINTIKQKQNNYKEKINNTRFLLLRNITSQLYAYQSNLDK
                     TINTFTQLTNAKINKSSITLIQIINTIKYQFISALEKTNNLLKNYNFSLKTNSKNIIN
                     IENCKLDNIPLILRHKSKSHLNYNLLRLKQINKLIPINLKQNLSDIKISLNKAQQIID
                     LSSPNHILKMGYVMLKKDNIIVTENNINKNDTIVLEGYKNQMEAEIINIEKK"
     gene            4747..4977
                     /gene="xseB"
                     /locus_tag="PKI65_04475"
     CDS             4747..4977
                     /gene="xseB"
                     /locus_tag="PKI65_04475"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOB77729.1"
                     /translation="MNNEELNLPTSYEQAFNELQQIVTEIERGDITIDLLLPKVQRAA
                     QLIKFCRDKLRNTEDEVNKIIKQLEDDNCDDM"
     gene            complement(5291..5878)
                     /gene="yihA"
                     /locus_tag="PKI65_04480"
     CDS             complement(5291..5878)
                     /gene="yihA"
                     /locus_tag="PKI65_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOB77730.1"
                     /translation="MKIKSVKYVKSCVSLTDCPKNILPEFAFVGRSNVGKSSLINALV
                     SQKIALTSSIPGKTKTINFYLIDNKWYLVDLPGYGYAKTSKTIRKQLINIINEYLSFR
                     SQLFLAFVLIDSSIPLQQIDIEFINNCGRYNIPIAIVLTKTDKEKKSIIQQNRKALMT
                     ELSKYWDELPPIFMTSSVKKTGIQDLLDYILNQIK"
     gene            complement(5859..6809)
                     /locus_tag="PKI65_04485"
     CDS             complement(5859..6809)
                     /locus_tag="PKI65_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOB77731.1"
                     /translation="MEQIIEPIDRELLKKELTRDIYVRKTNYNKNEIYIFAAEQCPNL
                     MLEVGRLREISFRDAGAGTGKKVDIDKFDTGPLPYKQLIVWDPREEEIIGGYRFILVK
                     DVLNFYNGKLHLATSHMFDFSDLFIEKYAPYTIELGRSFVQPKYQPQKNQRKGIFSLD
                     NLWDGLGALTIDYPEIKYFFGKVTLYLNYNQLARDAIVYFMYKHFPDTEKLITPKMPR
                     GYSNDISIFDDIFFSDSYEVNYKKLLEFVKSQGETIPPLINAYMSLSRTMKTFGTSLN
                     PQFGPVEEIGILVTIDDVYPSKIKRHVDSYLKEKNENKIS"
     gene            complement(7158..7721)
                     /gene="thpR"
                     /locus_tag="PKI65_04490"
     CDS             complement(7158..7721)
                     /gene="thpR"
                     /locus_tag="PKI65_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOB77732.1"
                     /translation="MKRLFIALPIELNKTAKEEIINLQHQLAMYKIKWVSLENTHLTL
                     VFLGDTDEIHLSTICDAIDSLESEFHKYQIELAGLSIFGNKRSPRVLWTRWLDNDETK
                     HLALSLQNKLTPLLSITEKNENFVPHLTLGRIKSLSNIDNFYDVLDKYSNFSFGKYII
                     DRIILFESKLTSRGPLYTPLHTVKAIT"
     gene            complement(7718..8944)
                     /locus_tag="PKI65_04495"
     CDS             complement(7718..8944)
                     /locus_tag="PKI65_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOB77733.1"
                     /translation="MLKNIKEDKNVIFVNKDVAWIGVLDYDIITFDVVMETRYGTTYN
                     SYFINAQMPTIIETTKETFWDTYIAKIRQVIEPEKIKYIILNHTEPDHSGNLLNLLKL
                     APEAKVVGSGIAIRNLRELFNADFPNIIVKGDDILDLGDKTLKFISAPNLHWPDSIYT
                     YLIEDKLLFTCDSFGCHYADDRMIDNEVGNFDDAFKYYFDVILKPFSKFMLQALDRIK
                     DLEINAILTGHGPLLLNDWKRYIEWTKELSEQFINTQNHNDIFVAYVSAYSKTASLAQ
                     AIAEGVKEEGLEVILKDIEHCSLGELEEYITRSRGMAIGSPVINQNILLPIYKLFALI
                     NPIRDRGKYLLAFGSFGWSGENQKLIPNIANGLKLNVLEESIFTKFTPNETDMNKAKE
                     LGKKLAQKVKQNTIKK"
     gene            9251..11029
                     /locus_tag="PKI65_04500"
     CDS             9251..11029
                     /locus_tag="PKI65_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HOB77734.1"
                     /translation="MSRKTLKIRDLTLRDGQQSLFATRMSQEQVDKTLPYFKEAHFYA
                     MEVWGGAVPDSIMRFLNEDPWERLAKIKKGIGDTSKLTALSRGRNLFGYNPYPEWVIE
                     GFIKNAINEGIDILRIFDALNDLDNCKSSVEYCKKYGAIPDGAICYTVDPVLKSKTIF
                     GKIFNKSHPKVFTTEYFVNKAKTFESMGAQIVTIKDMAGLITPKAAYEIITALKKELT
                     IPVNFHSHSTPGYGLASTLSAIIAGTDIIDTNIFPFAGGPAATAFEIVYLFCKKLDID
                     VNVNIEVIPKLQEILMDIRRELKQYDQYPNNYPKMFDPNNPNLPLEIEKLFDQAIELT
                     YKNKFEKALEVCQQIEAYFNFPPPNEAVKNAQIPGGMYTNMLTQLQQLNLEHLLPKVL
                     KAVPKVRIDAGCVPLVTPTSQIIGVQAVNCILDEKKGLPWYTTKSTQFIKLIEGAYGK
                     TPIPIDPKFRKFITGSETEKPFDTSTYLPFPNKEFPEYGNRKLYNNEKEQLLLELFPN
                     VAEEFLRKRAEQAYKKEIEEALKEKELEENKKRQALLREKEKYEKLSPEEKEKRLLYG
                     LYNYWSLPIIDTTDDDDLIETEYYII"
     gene            complement(11137..12870)
                     /locus_tag="PKI65_04505"
     CDS             complement(11137..12870)
                     /locus_tag="PKI65_04505"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOB77735.1"
                     /translation="MVGYNIDREILEKIENWLQQPIDEKDKEWIKNNLENNPKALVEA
                     FYADLEFGTGGLRGIIGIGSNRMNKYTVSIATQGLANYIKQNADKPFKVVISYDSRIM
                     SKEFAEITADVLSANDIEVFLFSELRPTPLLSYAVRYLKATAGVMITASHNPKQYNGY
                     KVYWSDGGQLVPPHDKGVIDEVKKVGEYSNVARDFNKSLIHIINDEIDHAYLKQLNNL
                     QFLAMVKPKTQKIVFTPLHGTGITMVPKALNDWGFNQIHLVEEQSIPDGNFPTTVSPN
                     PEEKAAMELALKNAKKINADLVLATDPDADRVGTGIKLPDGNYKLLNGNQIASLLTYY
                     NLEMLKSRNALPSNSFIVKTIVTTELMRKIADDYNVKTYDVLTGFKYIAELIRIKEGK
                     EKFICGGEESYGFLSADWVRDKDAIMTSCQIAEMQEYADRNGKTLLDILKILYETYGY
                     FYEDQKSIAMTGIEGMENMKKLMDNYRSNPPMEINNTAVVKILDYQLSIAKDLINNTT
                     QAILLPKSNVLQFILENDTKITIRPSGTEPKIKYYFAIVKKNINNKSYIEMWKELEKE
                     MAKLKEFADKI"
     gene            complement(13011..13874)
                     /gene="rfbD"
                     /locus_tag="PKI65_04510"
     CDS             complement(13011..13874)
                     /gene="rfbD"
                     /locus_tag="PKI65_04510"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOB77736.1"
                     /translation="MIYKILVTGSDGQLGYELKRLLSDNKNFELFFTNHKTLDITDEQ
                     AIRDFYNKYKPNFVINAAAYTAVDKAETEKEIALKVNVNGVRNLAKYSIDYNTIMIHI
                     STDYVFDGNKSSPYIETDTPNPLGYYGYTKFMGENEMSKINPRGVIIRTSWLYSNHGN
                     NFVKTIIKKSLDENQKLQVVYDQIGTPTYARDLAMCIYQMIQCFDFNNLEFFHFADEG
                     VASWYDFAHAIIRIKNIDKCIYPIETDQINQLTMRPKYSVMNKSKIKSALNISIPHWY
                     DSLKEMLGLFM"
     gene            14351..15739
                     /gene="mgtE"
                     /locus_tag="PKI65_04515"
     CDS             14351..15739
                     /gene="mgtE"
                     /locus_tag="PKI65_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOB77737.1"
                     /translation="MENKNDFLNIKEEVEELGIEHILAILPILRKMPPVEVAEILNEL
                     DAEAIAFILKNFDIEQQGLIFSEFNISRQLELFNLFNKREFADIFEHMPPDDYVDLYQ
                     HLEPEEQVELLPYLSLDIRENVIRLSKYPEETAGGIMTTDFFTVHSGKTCEELINLIR
                     SRSPSNRALYHIYVINEDNVLLGIINLEDLIKAKGDTLVDDVMEKDYEFVFVDEDREK
                     VAEIIAKYDLITLPVLNHDYQLIGVVTSDDAIEVIHSEQTEDFEKFMGIVQEPEYFDY
                     LGTPTTVHFKKRIVWLIALAIIELFAGMVINHYQPLIQQLYVLALFMPMLTAMGGNAG
                     SQSATVIVRALALGQITVKEWFKIIWKELRIAVLLALILGILTFANAMILNSFRVIPE
                     GFSISYISFVIALSMSLQIICSTFLGALSPLIVKSLKGDPALAASPAIATLVDITGLI
                     IYFSVASLLLGL"
     gene            complement(16204..16377)
                     /locus_tag="PKI65_04520"
     CDS             complement(16204..16377)
                     /locus_tag="PKI65_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77738.1"
                     /translation="MQHLQYLPYGELFISQRNTEEFDSRYKFTAKELDNETSYTYFGA
                     RYYDSELSISNQN"
     gene            complement(16740..17639)
                     /locus_tag="PKI65_04525"
     CDS             complement(16740..17639)
                     /locus_tag="PKI65_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77739.1"
                     /translation="MNKILNNCRIQRLFILIIVISNNIFSQKNLIYNGSFEEYYSCPC
                     NLNVVDTIPCIGWWSPNDATPDYFNKCWIELPHTNFCLYNYIYLASYKPKTGNGYMGL
                     GLIGEENFWMEHIQSKLIEPLEAGKQYKVSFWVKLAYKYSDYAAYNIGLYFSKDSDIV
                     GKKYNVSSYVRYMTPELRAHIKNPDGNFIIDTNWVEISGIYTAQGGEQYITIGMFWDD
                     NPAVVEAHKKYKNKNTQYNKKLLSKAIKNNLLVENPYIEKYKKMEDQYIRSHSPYYYH
                     YWDKEKKNIYYFIDDVSVIELDE"
     gene            complement(17632..18138)
                     /locus_tag="PKI65_04530"
     CDS             complement(17632..18138)
                     /locus_tag="PKI65_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77740.1"
                     /translation="MKKLKAFCILLLLNLVVSVSILANNNLDNKINYEFFYQGKDTIY
                     YTTGEYRISRIKSIRHAYLIYLSNSTMDILYTLISPKCNVRLPEKLKEGETYIMQIIY
                     LVPTQKYARYVGHKFITKLTMLDKKVRISRRNLWGTIIISPNVLDKYYRKDHFLGPEV
                     FKEANNYE"
     gene            complement(18143..18598)
                     /locus_tag="PKI65_04535"
     CDS             complement(18143..18598)
                     /locus_tag="PKI65_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOB77741.1"
                     /translation="MKIHYHSDHLGSASFVTNAEGAAVQHLQYLPYGELFVSQRNTEK
                     FDSRYKFTAKELDNETSYTYFGARYYDSELSAWLSVDPMSDKYPSLSPYCYSADNPVV
                     LVDPNGEKVYIVGDAADEATGFLIKKLYKFSQPPYNKPSSIHDQINNNK"
     gene            complement(18931..21348)
                     /locus_tag="PKI65_04540"
     CDS             complement(18931..21348)
                     /locus_tag="PKI65_04540"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOB77742.1"
                     /translation="MQKNELNKGNLLFLLLILLSNSLLSQEKIITGKVLDSITLTPLP
                     FVHIYYLDSNIGTITDINGNFTIKLEDDKKNLVFSFVGYKKDTLSVDSIKKTNIIKLA
                     PIQYLLKEVVITPGPNPANRIIELAIANKEKNNPLNYNNFSCTAYRRFVITTSIDSIK
                     NNNRLEDSIKDEYINFFERYHFFLTESVSEIIYKYPDDWHEKVIDTKISGIDDPTISV
                     LFNQLTPFSFYSKTINIGDKHYLNPINANSTKYYYFSLKDTIYDGRDTIFVIHYQPYK
                     NKNFEAIEGLLYISSNRWAIKSVTATPYENTPGLLMNITQNYELIDSLWFPKELKTEI
                     WFTGFRIGGSPLFANGETFLYDININKPDFKKSFNLFDTEVDLKSINKNDLLSLYRNT
                     KDSQIDKNTYDLLDSIAKNSNFNKLMNITTSLMNGFIPLSIFNLPLSDILQYNVFENW
                     RLGLGFVTNDKLSERFILDCSMGYGLGDKKWKYSIAGIAYPLKNFRWDIRLGYKFDIN
                     RLGQVTFQEQQLNLTAPENFWIYYLNKAEYLKSVFLTSNYFLNKKLLLSINFNKNQLS
                     FYPDYYYGWKENNVFIFTDKYDYHAVELKLQWCGQTKRIKTPISIVSFNDIEAKNIYG
                     YYRFTIDNTKKATHLFALKFQWLKKMNFSGRIGLDVEIGTIWGDPPISLTYSPISKYE
                     FFAVSSPYTFQILTPNSFFASSFATAHLCYYSNKLRISRYSNPYFVPHLSFHIGNLNK
                     GIQKHYNNPIPVADKGYWELGLNISNILQTPFYSIGIGAAYKLGAYKEKNWKNNTTIL
                     LVLDYPF"
     gene            complement(21391..22404)
                     /locus_tag="PKI65_04545"
     CDS             complement(21391..22404)
                     /locus_tag="PKI65_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOB77743.1"
                     /translation="MNNKKFILFLILIFTLLGCTNKKYLFNGETQGSTYSIIFYSDKD
                     KQFINNVSKIIDSILFHIDMVASVFNDSSEISIFNINDTLIDFSDDFLFLYNKSKEIY
                     YNTDGIFNPAIGPLIKYYGFLKDKGTDKIDTQYVKQLLCFTNLDSIKINVEQRKITKP
                     KGYYLDFNAIATGYTVDKIAQNFDKLGIENYLIEIGGEVYAKGSHKNKKPWKVGIEKP
                     PVSMYSPQNVLLKVAIKDKAIVTSGITRKYHSNNNKKINHIIDPQTGFPIPSNILSVS
                     VIAPNCTDADAYATAFMIMGVDNTLNFIKNMPPYEVFFIIVNKDSFDIKYSDGFANYI
                     ISH"
     gene            22471..23118
                     /locus_tag="PKI65_04550"
     CDS             22471..23118
                     /locus_tag="PKI65_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77744.1"
                     /translation="MKKVKLLIILLIALISFSCDQDKETEVPEPKGIFFRGTIDGHKR
                     TIADGVNGYVVKYHDTCYVIDSFNYYEPFMILSQESQNYYVSSKEAISFTFKDLFLFH
                     DSITNKDSMLGAYLYNHKLIPIYSNASSNGSLIMGGLDIMWRDAEGREWFTGLGAQNS
                     IATIDTLVIVRKPGMISEYNMYITFDCKLYTLGASISKEIKDAHARISIKNTCFY"
     gene            complement(23112..24491)
                     /gene="rlmD"
                     /locus_tag="PKI65_04555"
     CDS             complement(23112..24491)
                     /gene="rlmD"
                     /locus_tag="PKI65_04555"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOB77745.1"
                     /translation="MSKNNKYIYQIHVDKFASEGKCLIVHEGKVGLVPKVAPGDIIDV
                     RIIKDYKNYFEAIPIKIHKSSSYRVQPICQHYNICGGCKWQHIDYKHQLKAKKAQVED
                     AFLKILHLKNCIPEVIPSKKIFYYRNKLEYSFTNKRWIYEDEKNINIDMRALGYHIPG
                     RFDKIFHVDECHLMDFYHNEVRNKIFEFSIADNIPFYNKKTHEGTLRSLIFRNNRVGD
                     WMVIIVISEFTKKVENLFNKLIDFFPNISWNYAISNKLNDSIDGLMAVNLHGKSYLSE
                     ILDSFTFHISPFSFFQINPEQSENIYKKMIEISDMNSDDVAWDLYCGTGTISLYAANI
                     AKNVIGIESNAEAVQLANKNAKINSIKNVKFIKGDVSKILENTHIDKPNIIFLDPPRC
                     GLSKDVINIIKNLKPRTIIYLSCNAATQARDISLFIDDYTINFIQPYDMFPQTAHVEN
                     LVKLTVKNQ"
     gene            complement(24498..25304)
                     /gene="cdaA"
                     /locus_tag="PKI65_04560"
     CDS             complement(24498..25304)
                     /gene="cdaA"
                     /locus_tag="PKI65_04560"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOB77746.1"
                     /translation="MWNILGQLLHLSITDLIDIILVAWIIYEFYMLIRGTIAFTVFLG
                     IILIVVLYQVVNIFHLPMFSAFLGQFISAGIIMLIIVFQNEIKRFLSLIGTPLQKTRI
                     FQQNDRKRFLFFKLPSYDTSEKTNEIIEAALNLSHEGFGALIAIEKNITLQDIIATGI
                     IINAEVNRKLIETIFYKNTPLHDGGMIIRKDTIIAARTIFPLAEESKFNDLYPSLGTR
                     HRAAIGLSEQSDAVIIVVSEQNGAISIAHQGKLFYNLNPIEFQNKLTSLI"
     gene            25415..25489
                     /locus_tag="PKI65_04565"
     tRNA            25415..25489
                     /locus_tag="PKI65_04565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25450..25452,aa:Val,seq:gac)
BASE COUNT         9033 a         3802 c         3516 g         9362 t
ORIGIN      
        1 aatcgaaaag ggtctagaaa caaatcattg ttttacttga agtagtttat ctatatttcg
       61 tattcaccag ccgccaatac tcatcccacg cccatctgtc cgaggatgcg agatttaaaa
      121 gcctaccaat gtctgattca atctcacttg tcaagtattt atgtgtgatt acaaaccaca
      181 tcagatagct ctgcaagtat ttggtagcca ctcctctaaa tcgtcgcaac caaaccttga
      241 aatcatctat atgcttatcg actacttgta cactgtatac accgcgtcta atctgcgttt
      301 tgtttacaac aacttttttg cttttggtcc ctttcacagc ttttttgaat tccttctttt
      361 gcttcacaca tattaagttg ctatctgtca ctctatgcga tagctcttct cttatctgac
      421 tattggttac ttttttgcgt cctacgtgtt tgaaaagtaa attgccttcc ctgtctgtca
      481 ttactagcac agctactttg ggatgtttct ttttctttgc tttttcgtat tcttttcggc
      541 ttctgtattt gcgacctttt tctgaatatt tcattagcag ctcatcagct tctacaatgc
      601 ctgagaagct gataccaccc tgtaatgatt tgatcgctgc tagtatcttg tgtcgccaat
      661 tgaaactcgt tctcagactt atgcctactt ccgatgcgca agctcttaga cttttgcctt
      721 ctaacatgca ttttaggtaa tcgagcattt tatcagcttt tcgcatgcga tatacgaagg
      781 ttctagctgt ttcgcggtat tgtcggccgc aatctttgca tttgtacact tgtttgccgt
      841 cgcgtactcc agcttttatt acattttcgc tttcgcatct aggacagatg ggggctgtgc
      901 tttgtatttt tagcttctga taatctacgg ctgtgtagtt gttttctaat aaaaattgat
      961 atagattatc tagaaatcta cgtttctgct ctttgggtag agcttgataa tctaataaat
     1021 taaaattatc cataattatt tttttgtaaa attaaataaa tattatttaa tggtaatata
     1081 aaatacgtaa agtaaaacaa agaataaatt ttgtaagccc tcttccacct aataaatagt
     1141 ctttatgaag cgtgattttg gtatttgttt agaattttta attattaaaa tcgatttata
     1201 aattggggat aatttctatt ttgcataatt ttgcaaataa taaaatcacg tataaatttt
     1261 ttgatgaaaa aatcaataaa attaaaaaaa ctagatttat atttaatcaa atcttttata
     1321 ggccctctgc tagtaacttt tattatagct atttttgtat tattaatgca atttttatgg
     1381 tcatatattg atgaattagc tggtaagggg ttatctatta gtgtaatatt gcaattaatg
     1441 ttttatgcat ctgctacttt tgtacctatg tcgctaccat tagctatttt attatcttca
     1501 ttaatgacct atggcaattt aggcgaaaac tatgaaataa tagctgctaa agcctcaggt
     1561 atttctttat ggaaacttat gaaaccatta gctatagttt ctatattatt agcttgtttt
     1621 accttttatt tttcaaatac catacttcct aaagctaatt taaaaatgat gtctctacta
     1681 cgtgatgtaa gagaacaaaa acctactcta aatattagag aaggtatttt taataaagat
     1741 ttagataatt atgtaataaa agttgagaaa aaacacaaag atgggaaaca tgtagaaaat
     1801 ataataatat atgatcactc tgactttgga cctgccctac gcattaccac tgctcaatct
     1861 ggtattatgg aaactacccc cgatgaacgt tatttgattt ttacactttt taatggtatg
     1921 agctatgtag aactcagaga aaacagagat gattatatac gaataccttt acaacgtatt
     1981 tattttgatg agcaagtttt gcatatcgat ttatctagtt tcgcttttca acgttcagat
     2041 cctagtattt taaaagaaca ttataaaatg ttaaatcttt cacaactaaa gtattatata
     2101 gattcactag atagagattt aaaaaattta atagaaaacc acactaatat gctccttatg
     2161 acatttaata atttctggat ttatgtagct aatgatagta atttcaaacc aatgcaaaat
     2221 ataactgtta atacttttga tagtttaata aatttattag acgacgatag tcatagagaa
     2281 atgtatagga gtgcatctga acacgtacta aacaatatga atatgataga caatcaagcc
     2341 gaactatata gatcaataaa aaaatcaaaa acatattacg aaatagaatg gcatagaaaa
     2401 tttacacttt cactcacttg tttactttta ttttttattg gtgctcctct aggtgctata
     2461 ataagaaagg gtggaatggg cttcccttta gtagtttctg taatagtttt tgtcctttac
     2521 tatattatta ctatcttatt tgaaaaaact gctaaaaata tgctcatatc tcctttttta
     2581 ggaatgtggt tgcctacttt tattttatta acttttggct tatggatatc tattaaaacc
     2641 attaaagata gccctttgat ggaagctgat gcttggatta aaaaaattaa taatattaat
     2701 caatatttta aaaaattgtt taaaattaaa aataataata aatggcatta atttttaata
     2761 atttaaatgc aattaaaagt cttaatacta attttttaaa taaaataaaa aaaataatta
     2821 agcaaattct aaatgattat gaatttaaag aaggtaaaat cataattaat ttggtaaatg
     2881 acaatgaatt attacaaata aataaaaatt ttctaaatca tgactattac acagatataa
     2941 tagttttcaa tgaaattaag aataattatt tgtttgctga attatatatt agctatgata
     3001 gattaataga aaatgcaaat aaatacaatc aatctattga aaatgagact gcgagactta
     3061 tcatacatgg cattctgcat agttgtggtg aagatgataa tgatgaaaaa tctaaacaaa
     3121 gcatgacaat aaaagaaaat tattatttga atttttacta ataaatgatt tttagctcaa
     3181 gtttttattc taacaattat tgaaaactta tttttatttt aactttgtat aatatggaaa
     3241 attttattac tctaaaagaa ttagctaaac aaatagaaag tattataaat ctgaatttca
     3301 acaatacata ttgggtattg ctggagatac aaaaattaaa tctttatcgt actggtcatg
     3361 cctatccaga gcttgtagaa aaagatgaaa aaacaaataa aatagttgct cagatgcgtg
     3421 gtattatatg ggctaatgat tatttatata tccaacgaaa atttatacaa gtcttaggaa
     3481 gtgaaatgaa ggatggtatc agagtattag ctaaatgcat tgtcaatttt catcctgtat
     3541 atggactaag ccttagaatt atggatatag atactacata ttctcaaggc ttattggagc
     3601 aggagcgtct acttacaatc aaaaaactac agcagcaagg cttattagat gctaataagc
     3661 aaataccttt cccattggta ccacaacgaa tagcaattat ttcaatagaa actagtaaag
     3721 gatttagcga ttttgtagga gtattagcaa ataatgaaca ctgttatagt tataaataca
     3781 cccttttccc agcagtatta caaggtgaaa aggctgtaga gaccattacc gaacaactga
     3841 aaatcataaa tctaaaaaaa gatttttatg atatagtagt gatagtgcgt ggaggtggtg
     3901 gcgaaatagg tctatccgct tataatgatt attcactagc aaaagcaata gctactttcc
     3961 cacttccagt tttgactggt atcggacatt ctactaacct cactgtctgc gatatggtgg
     4021 ctcactattc agccattaca cctaccgacc tgggatatta tattatcgat aaaacattgg
     4081 aatttgaaaa tatgttacta aatattggcg acaaattaat caatacaatt aagcaaaaac
     4141 aaaataatta taaagaaaaa ataaataata ctcgattttt attgttacgt aatataactt
     4201 ctcaattata tgcttatcaa tctaatctcg acaaaaccat taatactttc actcagttga
     4261 ctaatgctaa aatcaataaa tcatcaatta ctctcataca aattattaat actatcaaat
     4321 atcaatttat ttctgcctta gaaaaaacta ataatttgtt aaaaaattat aacttttctc
     4381 tcaaaacaaa ttcaaaaaat attattaata ttgaaaattg taagcttgat aatataccac
     4441 ttatacttag acataaatct aaatcacatt taaactataa tctgttacgg ttaaaacaaa
     4501 taaataaatt gataccaata aatctaaaac aaaatctatc tgatatcaaa atctcactta
     4561 acaaagctca acaaatcata gacctcagct cgcctaatca tattttaaaa atggggtatg
     4621 tgatgttaaa aaaagataat ataatcgtaa ctgaaaataa tataaataaa aacgatacaa
     4681 tagttttaga aggttataaa aatcaaatgg aagccgaaat tattaatatt gaaaaaaaat
     4741 aaaaatatga ataacgaaga attaaattta ccaacaagct acgaacaagc ttttaatgaa
     4801 ttgcaacaga tagttaccga gatagaacgc ggtgatatca ctatagatct tttattacca
     4861 aaagtacaga gagcagctca actaataaaa ttttgcagag acaaacttag aaatactgaa
     4921 gatgaagtta ataaaatcat aaagcaacta gaagatgata attgcgatga tatgtagtga
     4981 aactaaagat atttaaaacg taaaatgaca caatcataat gaaatgataa atttaaatac
     5041 aatattatct aaaattgaaa gtaaataaac ggttgtaaat ctgcagtaat aaattgaaaa
     5101 actaaataat ataccacagc atcatattgc cctgaataac gcagctccat atttgagata
     5161 ccaaaagcta aatactttag ctcattagat agctttctag cataaaatta cagaatcaat
     5221 agaaggttaa tgtttataaa aagtattcaa atttctcaac tcagtatcat ttaaattatc
     5281 agtcaaatac ttatttaatt tgatttaaaa tataatctaa taaatcttga attcctgttt
     5341 ttttaacaga ggatgtcata aatatggggg gcaattcgtc ccagtatttt gataattcag
     5401 tcataagagc ttttcgattt tgttgaataa tacttttttt ttctttatca gttttagtca
     5461 acactatggc tataggtata ttataccgtc cacaattatt tataaactct atatctattt
     5521 gttgtaatgg tatcgaggaa tctattaaaa caaatgctaa aaatagctga ctacgaaagc
     5581 taagatattc gtttatgata tttattaact gcttacgaat agttttagat gttttagcat
     5641 atccatagcc aggtagatct actagatacc atttattatc tatcaaataa aaattgatag
     5701 ttttagtttt accaggtata gatgatgtta atgcaatttt ttgagaaact aatgcattta
     5761 ttaatgaaga tttccctaca ttagagcgac ctacaaaagc aaactctggc aaaatatttt
     5821 taggacaatc tgttaaagaa acgcaacttt ttacatattt aactgatttt attttcattt
     5881 ttctctttta gatatgaatc tacatgcctc tttattttac ttggatatac atcatctata
     5941 gttactaata ttccaatctc ttctactggg ccgaattgcg gatttagaga agttccaaaa
     6001 gttttcattg tacgcgacaa actcatatat gcatttatca atggggggat agtttctcct
     6061 tgagatttta caaattctaa tagtttttta taattcacct cataagaatc agaaaagaat
     6121 atatcatcaa aaatcgatat atcattcgaa tagccacgtg gcatcttagg agtaattaac
     6181 ttttcagtat caggaaaatg cttatacata aaatatacaa tggcatcacg agctaattga
     6241 ttgtaattga gatataaagt taccttgcca aagaaatatt taatttctgg ataatctata
     6301 gtaagagctc ctaggccgtc ccacaggttg tctaatgaaa aaatcccctt tcgctgattt
     6361 ttttgtggtt gatatttagg ttgtacaaag ctcctaccta attcgatagt atacggtgca
     6421 tatttttcta taaatagatc agaaaaatca aacatgtgag aagtagctaa atgtaatttg
     6481 ccattataaa agttaagtac atcctttacc aatataaatc tgtagccacc tattatttct
     6541 tcttctcgag gatcccaaac tattaattgt ttatatggca aaggacctgt atcgaatttg
     6601 tctatatcta ccttcttgcc agtgccagct cctgcatctc taaaagatat ttctcgtaat
     6661 ctacctactt ccaacattag attgggacat tgctcagcag caaatatgta aatttcattt
     6721 ttattgtaat ttgtttttct tacataaata tcccttgtca attctttttt taataattct
     6781 ctgtcaattg gttctattat ttgctccata ttaatagtta aaaaataagt ttattttttt
     6841 taaaattttt accaaaatta taaaacttta ttgaaattta gatgcaaaat atattctgca
     6901 taactattaa tattgtcatt atatactaaa ataatattgt tttatcttat tattctacgc
     6961 attcctcata ttagttttta ataataaatt caaatatcaa atgtactaaa taaattggta
     7021 aagtgcgaat gggtaacaag ttgtgaaaat aagagaatga atctaagcga ataaaagaag
     7081 ggtatttaat agtattataa aatatttaat ctttgtgtaa ttttttagca tctattaaaa
     7141 aaaattaata aattatgcta tgtaatagct ttaactgtat gtaatggagt atatagtggg
     7201 cctcgtgagg ttaatttgct ctcaaaaagt attattctgt ctattatgta tttaccaaaa
     7261 gaaaaattag aatatttatc taacacatca taaaaattat ctatatttga taagcttttg
     7321 atacgtccca aagttaaatg tggcacaaaa ttttcatttt tttcagtgat agaaagtaat
     7381 ggggttaact tattttgtaa tgataaagct aaatgtttgg tctcatcatt atctaaccat
     7441 ctagtccaca aaacacgagg cgatctctta ttaccaaaaa tagataatcc agctaactct
     7501 atctgatatt tatgaaattc agattctaag ctatctatgg catcgcagat ggtagataga
     7561 tggatttcat cagtatctcc tagaaataca agagtcaaat gcgtattctc taatgatacc
     7621 catttgattt tgtacatagc taattgatgc tgcagattaa taatttcttc ttttgcagtt
     7681 ttatttaatt caataggtaa tgcaataaat aaacgtttca ttttttaatg gtattttgct
     7741 taactttttg agctaatttt ttacctaatt cttttgcttt attcatatca gtttcattgg
     7801 gagtaaattt agtaaatatg gattcttcta atacatttaa tttcaaacca ttagcaatat
     7861 taggtatcaa tttttgattt tctccgctcc atccaaaaga tccaaaagcc aaaagatatt
     7921 tacctctatc acgaatagga ttgataagag caaaaagctt ataaataggt aaaagaatat
     7981 tttgattaat tacaggtgaa ccaatagcca taccacgaga tctagttata tactcctcga
     8041 gttctcccag agagcaatgc tcaatatctt ttaaaataac ttctaaccct tcttccttga
     8101 cgccctcagc tatggcttgg gctaatgatg ctgttttgct ataagcagat acataagcta
     8161 cgaatatatc attatgattt tgagtattga tgaattgttc tgataattct tttgtccatt
     8221 ctatgtatct tttccaatca tttaagagca aaggaccatg tccagtgagt atagcattta
     8281 tttctaaatc tttgatccta tccaaagctt gtaacataaa tttactaaaa ggctttagaa
     8341 tgacatcaaa ataatattta aaagcatcat cgaaattacc cacttcattg tctatcattc
     8401 ggtcatctgc atagtgacaa ccaaagctat cgcatgtgaa aagaagctta tcctcgatca
     8461 aatatgtgta gatactatca ggccaatgta gattaggagc agaaataaat ttcaaggttt
     8521 tatcccctaa atctaatata tcatcacctt ttacaataat attaggaaaa tcagcattga
     8581 ataattctct taaatttcta atagctattc cactacccac taccttggcc tcaggagcca
     8641 gttttagcag atttaataga ttaccactat gatctggttc tgtatgattt aaaataatgt
     8701 attttatttt ttctggttct atcacttgcc ttatcttggc aatataagta tcccaaaaag
     8761 tttcttttgt agtttctatg attgttggca tctgagcatt tatgaagtaa gaattgtatg
     8821 tagtaccata acgagtctcc atcactacat caaaagttat tatatcatag tctaatactc
     8881 ctatccaagc tacatcttta tttacgaaaa ttacattttt gtcttctttt atatttttta
     8941 acataattgt ttgatttttt ttttgataat gcaaatataa tattataatt aaaaataata
     9001 caaggtaaat gataaatatt aacctaaatt aaaattttta ataagaataa atctaaaaaa
     9061 acaattgtaa tctaaagtac atgaataatt agaaataata ttaaaaaata aattactata
     9121 aaattaaaaa aaaatagtaa aaaataggtt gatttcatta aatatagttt taaatctcaa
     9181 taaaaataga attttataaa ccagatatta aataaatagt taaatttgca aataaaatat
     9241 ttaaattaat atgtcacgaa aaacactaaa aatcagagat ttaactttaa gagatggaca
     9301 acaaagtctt tttgcaaccc gcatgagtca agaacaagta gataaaacac tcccatactt
     9361 caaagaagca catttctatg ctatggaggt atggggcggt gctgttcctg acagtataat
     9421 gcgttttttg aatgaggacc catgggagag attagctaaa ataaaaaaag gtataggaga
     9481 tactagtaaa ttaacagcac tatcaagagg tcgaaattta tttggatata acccatatcc
     9541 tgaatgggtt atagaaggtt ttataaaaaa tgctataaat gaaggaatag atatattaag
     9601 aatttttgat gctttaaatg atctagataa ttgcaaatct tcggtagaat attgtaaaaa
     9661 atatggtgct atacctgacg gggcgatttg ctacactgta gatcctgtat taaaatctaa
     9721 aactattttt ggtaaaattt ttaataaatc tcatcccaaa gttttcacta ctgaatattt
     9781 tgtcaataaa gctaaaacat tcgaatctat gggagctcag atagtaacaa taaaagatat
     9841 ggctggatta ataactccca aagctgctta tgaaataata actgctctca aaaaagaatt
     9901 aactatacct gtaaattttc attctcatag tactcctggc tatggtttag catctacact
     9961 atcagctata atagctggaa ctgatatcat agatacaaat atttttccat tcgctggtgg
    10021 acccgccgct acagcttttg aaattgtcta tcttttttgt aaaaaattag atattgacgt
    10081 aaatgttaat atagaagtaa taccaaaact acaagaaata ttaatggata tacgtagaga
    10141 attaaaacaa tacgatcagt atccaaataa ttatcctaaa atgtttgatc ctaataatcc
    10201 caatttacct ctagaaattg aaaaattatt tgatcaagca attgagttaa catataaaaa
    10261 taaatttgaa aaagctttag aagtatgtca acaaatagaa gcatatttta attttcctcc
    10321 acctaatgaa gctgtcaaaa atgctcaaat tccaggtggt atgtatacaa atatgttgac
    10381 acaattacaa caattaaacc tagaacactt gctaccaaaa gtattaaaag cagtacctaa
    10441 agtaaggata gatgctggct gtgtgccttt agtcacacct actagtcaaa taattggtgt
    10501 acaagctgtt aattgcattt tagatgaaaa aaaaggtcta ccatggtata ccactaaaag
    10561 tacacaattt attaaattga tagaaggtgc ttatggcaaa acccctatac ccatagatcc
    10621 taaatttcgt aaatttatta caggaagcga aacagaaaaa ccctttgata ctagcacata
    10681 cctgccattt cccaataaag aattccctga atatggtaat agaaaacttt ataataacga
    10741 aaaagaacaa ttattactcg aattatttcc gaatgtagca gaagaatttt taagaaaacg
    10801 tgcagagcaa gcttataaaa aagaaatcga agaagcactt aaagaaaaag aattggaaga
    10861 aaacaaaaaa agacaggcac tgctacgtga aaaagaaaaa tatgaaaaat tgtcacccga
    10921 agagaaagaa aagcgtttat tgtatggctt atataattac tggtctttgc ctatcataga
    10981 tacaacagac gatgatgatt tgatagaaac tgagtattat ataatttaat tagtaacaaa
    11041 aaaaattcta atataactta aaaattttag tataaaaaga atttatggat tagaaaaaaa
    11101 ttaactaaaa tctatgattt tagatttttt aattctttag atcttatcag cgaattcttt
    11161 caatttagcc atttcttttt ctaattcttt ccacatttcg atgtaggatt tattattaat
    11221 attttttttg acaatagcaa aataatattt gatttttggc tctgttccag aagggcggat
    11281 agtgattttg gtatcattct cgagtataaa ttgcaataca ttacttttag gtaataaaat
    11341 agcttgagta gtattattaa tcaaatcttt agcaatagaa agttgataat ccaagatttt
    11401 cacaacagca gtattattta tctccatagg tggattactt ctataattgt ccataagttt
    11461 tttcatattt tccatacctt cgataccagt catagcgata gatttttgat cttcgtagaa
    11521 atatccatag gtttcataaa gaatttttaa tatatcgagt agagtctttc cattacgatc
    11581 tgcatattct tgcatttctg ctatttgaca agaggtcatt atagcatctt tatctcttac
    11641 ccaatctgca gataaaaatc catagctctc ttcaccacca cagataaatt tttctttacc
    11701 ctctttgatt ctaattaatt cagctatgta tttaaaacca gttaaaacat cataagtttt
    11761 cacattataa tcatcagcaa tttttcgcat caattcagta gttacgatag tttttacaat
    11821 aaaactatta gaaggtagtg catttcgaga tttcaacatt tctagattat aataagtcaa
    11881 aagagatgct atctgattac cattcaataa tttataatta ccatcgggca atttaatccc
    11941 agtacctact cggtcagcat caggatcagt agctaataca agatcagcat ttattttttt
    12001 agcattttta agagctaatt ccatagctgc tttttcttca gggtttggtg agactgtagt
    12061 aggaaaatta ccatcaggaa ttgactgctc ttctacaaga tgtatttgat taaaccccca
    12121 atcattcaat gctttaggca ccatcgttat accagtacca tgaagtggtg taaaaacaat
    12181 tttttgagtt tttggtttca ccattgctaa aaattgcaaa ttgtttaatt gctttagata
    12241 agcgtggtct atctcatcat ttataatatg tattagactt ttattaaagt cacgtgctac
    12301 attgctatat tcacctactt ttttcacttc atctattact cctttatcat gaggtggcac
    12361 cagttgtcca ccatcactcc aataaacctt gtatccattg tattgtttag gattatgaga
    12421 tgcagtgatc atgactccag cagtagcttt caaatatcta actgcataag acaaaagtgg
    12481 agttgggcgt aattctgaaa ataaaaaaac ttctatatca ttcgcactca aaacatccgc
    12541 agttatttca gcaaattctt tgctcataat tcgagaatca taagatatta ctactttgaa
    12601 tggtttatct gcattttgtt taatataatt ggccagcccc tgcgtagcaa tagacacagt
    12661 atatttattc atcctattag aacctattcc tattatacct ctcaaacctc cagtaccaaa
    12721 ttctaaatca gcataaaatg cttctactaa tgctttagga ttattttcta aattattttt
    12781 gatccattct ttatctttct catctattgg ttgctgaagc caattttcaa ttttttccaa
    12841 aatttctcta tcaatattat accctaccat atctatatat ttttatcaat taatctaaaa
    12901 atagtaacaa atttataaat aattaaaaaa atcaaattaa taaaaaaaat tattataaaa
    12961 atcataatct taatattttt tttttaaaat actcaagttg atttttgatt ttacataaat
    13021 aaacctaaca tttcctttaa tgagtcgtac caatgcggta tcgaaatatt taaagctgat
    13081 ttaatttttg atttattcat tacgctatat ttgggtctca tagtcaattg atttatctga
    13141 tcagtctcaa tgggatagat acatttatca atgtttttaa ttctgataat agcatgagca
    13201 aaatcgtacc aagaagctac accttcatca gcgaaatgaa aaaattctaa attattgaaa
    13261 tcgaagcact gaatcatctg ataaatacac atagctagat cccttgcgta tgtaggcgta
    13321 ccgatttgat cataaacgac ttgcagtttt tgattttcgt caagcgattt tttgataata
    13381 gtttttacaa aattattacc atgattacta taaagccacg aagttctaat aataacacct
    13441 cgaggattta ttttcgacat ttcattttcg cccataaatt ttgtataacc ataatatcct
    13501 aatgggttag gtgtatcagt ttcgatatat ggcgatgatt tattaccatc aaaaacataa
    13561 tctgtagaga tatgtatcat tatagtattg taatctattg aatatttagc tagatttctc
    13621 actccattta cattaacttt taatgcaatt tctttttcag tttcagcctt atcgacagca
    13681 gtatatgctg cggcattgat gacaaaatta ggtttatatt tattataaaa atctctaata
    13741 gcttgctcat cagtaatatc caatgtttta tgattagtaa aaaataattc aaaattttta
    13801 ttatcgctta ataatcgttt aagttcataa cctaattgac cgtctgaccc agtaactaaa
    13861 attttgtaaa tcatggaata tagttttaat tttaaaaaaa tacaaacaaa ggtaacaaaa
    13921 aaatgaaaaa taaatattat atctaatttt ccaaatttaa ttaatgaaat taaaaaaatt
    13981 atttatttgt aaaataatat tatatatttt taataaaatt tgtatttttg caaaaaattt
    14041 tataaattat ttaatcatgg aaggggagtg taatcttact agtcattttt actttatttt
    14101 gtttccccat tgaaaaaaac aaaaggggta gtcctctacc ttgcttttta gttaggtggc
    14161 atgataatat tataactact aaatataaca aaatattttt tgcttctata aattttaata
    14221 aactattcga aattatttat attttatgtc ttgtcattcg agatttagac attatagttt
    14281 tttttaaaat ttttcttata tacttttaaa ataaaaaaag aacattaata atttttaaaa
    14341 aacaaaaaag atggaaaata aaaatgattt tttaaatata aaagaagaag tagaggaatt
    14401 aggaatagaa catatattgg caatattacc aatattgcgt aagatgccac cagtagaagt
    14461 ggcagaaata ttaaatgagc ttgatgctga agctattgcc tttatattga aaaattttga
    14521 tatagaacag caaggactta tattctcaga atttaatatc tcgaggcagt tagagttatt
    14581 caatttattt aataaaagag aatttgctga tatttttgaa catatgccgc ctgatgacta
    14641 cgtagacctc tatcaacatt tagaacccga ggagcaagtc gaacttttac cttatttatc
    14701 tctagatatc agagaaaatg taataagact aagcaaatac cctgaagaaa cagctggtgg
    14761 tataatgact acagactttt tcacagttca ctcaggaaag acttgcgaag agctaataaa
    14821 tttaatacgc tcaagaagtc catcaaatag agcattgtat catatatacg taattaacga
    14881 agataatgtc ttattaggaa taataaattt agaagattta ataaaagcca aaggtgacac
    14941 tttagtagat gatgtaatgg aaaaagatta tgaatttgtt tttgtggatg aagatagaga
    15001 aaaagtagca gaaataatag ctaaatatga tttgataacc ttaccagttt tgaatcacga
    15061 ctatcaacta ataggtgtag taacctcaga cgatgctata gaggtgatac attcagaaca
    15121 aaccgaagac ttcgaaaaat tcatgggtat agtccaagag cccgaatatt ttgattatct
    15181 cggtactcct actacggtac atttcaaaaa aaggatagtg tggctcatag ccctcgccat
    15241 cattgaattg tttgctggca tggtaatcaa tcattatcaa ccgcttatac aacagttata
    15301 tgttttagct ttatttatgc caatgttgac agctatgggt ggtaatgcag gtagccaatc
    15361 agctactgta atagtacgag ctttggctct aggacaaata accgtaaaag aatggttcaa
    15421 aattatttgg aaagaattgc gtatagcagt tttattagct ttgatcttag gaattttaac
    15481 atttgctaac gctatgatat taaattcttt tagagttatt ccagaaggtt tctccatttc
    15541 ttacatctct tttgtaatag ctctatctat gtcattgcaa attatttgtt ctactttttt
    15601 aggtgcttta tctcctttaa tagtgaaatc attaaaaggt gatcccgcct tagctgctag
    15661 ccctgctata gctaccttgg tagatatcac tggattgatt atttatttct ccgtagcttc
    15721 tttactttta ggtttgtaat agtttttatt atgttaatat ctaaatatat taaaagaaat
    15781 ttttaataaa ataattatat agattattaa ttaacatata gttgtaagaa taattttata
    15841 taaaatatgt tgaatagaat aaattaattt tgtaaaacga ataaagtttt ttgcctaata
    15901 acattaaaaa aaataagatt tattattttt tttattttga aaaaaatttg aaaaaaataa
    15961 atattgtatc cttcattatt gaattatcta gtctaaaaac tatcaagata gctatttgta
    16021 tatctatttt tatagaatat ttttatatgt tttaatagat ttttgcaatt cagtaaaagc
    16081 aggatgctta gattctttat ttaaaaaaaa tcctctatta tattaatcat cttacaaaat
    16141 tattgaaaaa tagagaaata acaaaacaat aaaaaggcga atttatcaaa aaaaagaaaa
    16201 ctattagttt tgattggaga tggagagctc gctatcatag tatctcgctc cgaagtacgt
    16261 gtagctggtc tcgttgtcta gctccttggc agtgaattta tatctactat caaactcttc
    16321 agtgtttcgc tgtgagatga atagctcgcc gtaggggaga tattgcaaat gctgcattac
    16381 tgcaccttct gcatttgtta cgaaggaggc ggagccgaga tggtcggagt ggtagtggat
    16441 tttcatttat aaatttttac taggtaaaat taatgaatat cttggaataa tttctactta
    16501 ttttactttt ctttctcagc tacacaaaga aaagtaacaa aagaaaggtc tccgctgggg
    16561 aaatgatttg ataaaactac gcaatcacct cttggggttt acaaactcgc tcgcttcgct
    16621 cgctcaaaca gtgtaaaccc ttcttcccaa cacggcttgc ttgttttatg gcaaatcatt
    16681 tgccaatgcg ggcgggacag tatgaaagaa attttttaag atataatcta aaaaaaaatt
    16741 cattcgtcca actctattac gctcacatcg tctatgaaat aatatatatt ctttttttct
    16801 ttatcccaat agtgataata atagggacta tgagaacgaa tgtattgatc ttccatcttt
    16861 ttatattttt caatataggg attttctact aataaattat tttttattgc tttactcaga
    16921 agctttttat tatattgtgt atttttattt ttgtattttt tatgagcttc aactacggca
    16981 ggattatcat cccaaaacat acctattgtg atatattgct ctccaccttg tgcagtatag
    17041 atgccactta tctctaccca atttgtatct attatgaaat tgccatctgg gtttttaata
    17101 tgtgctcgta actctggtgt catatacctc acgtagctag aaacattata ttttttacct
    17161 acaatatctg aatcttttga gaaataaaga ccaatattat atgctgcgta atcactatat
    17221 ttataagcta atttcaccca aaaactcact ttatattgtt tgcctgcttc tagtggctct
    17281 atgagtttac tttgtatgtg ctccatccag aaattttcct ctccaatcaa tcctaacccc
    17341 atataaccat ttcccgtttt gggtttgtaa cttgctaaat atatgtaatt gtacaagcaa
    17401 aaattagtgt gaggaagttc tatccagcac ttattaaaat aatcaggtgt agcatcatta
    17461 ggagaccacc accctataca aggtattgtg tctacgacat ttagattgca tggacagcta
    17521 taatattctt cgaagctacc attataaatt aaattttttt gtgaaaaaat attatttgaa
    17581 attacaataa ttaaaataaa taatctttgt attctgcaat tatttaatat tttattcata
    17641 attatttgct tctttaaaaa cttctggtcc taaaaaatga tctttcctat aatatttatc
    17701 taatacatta ggtgatatga ttattgtgcc ccatagattt cttctagaaa ttctaacttt
    17761 tttatccaac attgtcaatt tcgttataaa tttatgacca acgtatcttg catatttttg
    17821 tgtaggaact aaatatatta tttgcataat gtacgtctca ccttctttta gtttttctgg
    17881 taatctaaca ttacatttag gagatattaa agtgtataaa atgtccattg tagaattaga
    17941 taaatatatt aaatatgcat gcctaatact ttttattcta cttattcgat actcgccagt
    18001 tgtataatat atggtatcct tgccttgata aaaaaattca taattaattt tattatctaa
    18061 attattatta gctaggatac tcacagatac aactagattt aaaagtaata aaatacaaaa
    18121 tgcctttaat tttttcattt ttttatttat tattatttat ttgatcatga atacttgatg
    18181 gtttgttata tggaggttgt gaaaatttat atagtttctt aattaaaaaa cctgtagcct
    18241 catctgccgc atctcctaca atatacactt tctccccatt tggatccacc agcaccaccg
    18301 gattatctgc actatagcaa tatggcgata gcgatggata cttatcgctc atcggatcca
    18361 cgctcagcca cgcactcagc tcgctatcat agtatctcgc tccgaagtat gtgtagctgg
    18421 tttcgttgtc tagctctttg gcggtgaatt tatacctact atcaaacttt tcagtgtttc
    18481 gctgggagac gaagagctcg ccgtagggga gatactgcaa atgctgcact gctgcacctt
    18541 ctgcattagt tacgaaagag gcggagccga gatggtcgga gtggtagtgg attttcattt
    18601 ataaattttt gttaggtaaa attaatgaat attttggaat aaatttattt actttttatc
    18661 ctttgctagt ttgcataaat tatatgatct caaaatatat tatattcatt ttaaatatta
    18721 tattttctca tatttaaata tttctaaatt aggtattcaa tatgaaatta caaaaaaatc
    18781 aaattcgttc aataatatgg tgattattaa tctattaaaa aaaattatta ttagagtaaa
    18841 attcattact ccacggttct aatttctcaa acttctactg tgctatctat cttcttgcct
    18901 ctcgttactt ataaatataa aattttagca tcaaaatgga taatccaaaa ctaataggat
    18961 cgttgtgtta tttttccaat ttttttcttt gtatgcacct aatttatatg ctgcgcctat
    19021 tccaatagaa taaaatggcg tttgtaaaat attagaaata ttcaatccta actcccaata
    19081 acctttatca gctacaggta taggattatt ataatgtttt tgaattcctt tattcagatt
    19141 acctatatga aatgataaat gagggacgaa atatggattc gaatatcgtg atattctcaa
    19201 tttattggaa taataacata gatgagcggt agcaaaactc gaagcaaaaa aactattcgg
    19261 tgtcaaaatt tgaaatgtat acggtgagct tacagcaaaa aattcatatt tacttatagg
    19321 actataagtt aatgaaatag gtgggtcgcc ccatatggtt cctatttcga catctaaacc
    19381 tattctacca ctgaaattca tttttttcaa ccattgaaat tttaaagcaa aaagatgagt
    19441 tgccttttta gtattatcaa tggtaaatct ataataacca taaatatttt tagcttctat
    19501 atcattgaaa gatacaatag aaataggtgt tttaatcctc ttagtttggc cacaccattg
    19561 taattttaat tctacagcat gataatcata tttatcagtg aaaataaaaa cattattttc
    19621 tttccatcca taataataat caggataaaa agataattga tttttattaa aatttattga
    19681 caatagaagt tttttattta aaaaataatt agaggttaga aaaactgact ttagatattc
    19741 tgctttattc aaataataaa tccaaaaatt ttctggagct gttagattta attgttgttc
    19801 ttgaaaagtt acttgcccaa gcctattaat atcaaattta tagcccagtc taatatccca
    19861 tctgaaattt tttaatggat atgctatacc tgcaatacta tatttccatt ttttatcacc
    19921 taaaccataa cccatagaac aatcaagaat aaatctctct gataatttat cattagttac
    19981 aaatcctaat cctagcctcc aattttcgaa aacattatat tgtaatatgt cgcttaatgg
    20041 caaattaaaa atacttaaag gtataaatcc attcattaat gatgtcgtta tattcattaa
    20101 tttattaaaa ttactatttt tagcaatact atctaataaa tcataagtat ttttatctat
    20161 ttgcgaatct ttagtatttc tatataatga caataaatca tttttattaa tggatttaag
    20221 atctacttcg gtatcaaaca aattaaatga ttttttgaaa tctggcttat taatgttgat
    20281 atcatataaa aaagtttctc catttgcgaa taaaggggaa ccacctattc taaagccagt
    20341 aaaccatatt tctgttttta attcctttgg gaaccacaga gagtcgatta actcataatt
    20401 ttgcgtaata ttcattaata atcccggtgt attttcgtaa ggtgtagctg taacactttt
    20461 tattgcccat cgattggatg atatatataa tagaccttct atcgcttcga aatttttatt
    20521 tttataaggt tgataatgaa tcacaaatat ggtatctcgt ccatcataaa tagtatcttt
    20581 taatgaaaaa taataatatt tagtactatt agcatttatt ggatttagat aatgtttatc
    20641 tccgatattt attgtttttg aataaaaaga aaacggtgtt aattgattga ataaaacaga
    20701 aatagtagga tcatctatac ccgatatttt agtatctatt actttttcgt gccaatcatc
    20761 tggatattta tatataattt cagaaacact ctcagttaaa aaaaaatgat atctctcaaa
    20821 aaaatttata tactcatctt ttatactatc ttctaatcta ttattatttt taatagaatc
    20881 tatggatgtg gtaattacaa aacgcctata tgctgtacaa gaaaaattat tataatttaa
    20941 tggattattt ttttccttat tagcaattgc taattctata atgcgattcg caggatttgg
    21001 accaggtgtt attactactt cctttaataa atattgtata ggtgcgagct ttattatatt
    21061 tgttttctta atgctatcta cactcaaggt atctttttta taaccaacaa agctaaaaac
    21121 taaatttttt ttatcatcct ctaatttaat agtaaaattg ccatttatat ctgtgatagt
    21181 acctatatta ctatcaagat aataaatatg tacgaatggc aaaggggtga gagtaatact
    21241 atctaatact ttacctgtga taattttttc ttgtgaaagt aatgagttag ataataatat
    21301 aagcaaaaga aaaagtaaat tccctttatt caattcgttt ttttgcatca aaattatatg
    21361 aaatttttta tttattaatc tttatatgcc tcaatgtgaa attatgtaat tggcgaaccc
    21421 atcagaatat ttaatatcaa aactatcttt attaacgatt atgaaaaaca cttcatatgg
    21481 aggcatattt ttaatgaaat ttaatgtgtt atctactccc attatcataa aagcggtagc
    21541 ataagcatca gcatctgtac aattaggagc tataaccgat acacttaata tattagatgg
    21601 tataggaaat ccagtttggg gatctatgat atgattgatt tttttattat tgttactatg
    21661 atatttccta gtgataccag aggttacaat agctttatct tttattgcta cttttaataa
    21721 aacattttgt ggactataca tacttacagg cggtttctct atgcctactt tccatggttt
    21781 tttattttta tggctacctt tagcatatac ttcgcctcct atttctatta aataattttc
    21841 tatgcctaat ttatcaaaat tttgtgctat tttatcaacg gtataacccg tagctattgc
    21901 attaaaatcg aggtaataac ctttgggctt agttattttt ctttgctcaa catttatttt
    21961 tatagaatct aaattagtaa aacataataa ttgtttcaca tattgagtgt ctattttatc
    22021 agtaccctta tcttttaaaa aaccataata ctttattaat ggacctatag ctgggttaaa
    22081 tattccatca gtgttataat aaatttcctt acttttatta tataaaaata aaaaatcatc
    22141 actaaaatca atcaatgtgt cattaatatt aaaaatactt atttctgagg aatcattaaa
    22201 aactgaggca accatgtcaa tatgaaagag gatagaatca ataatcttac ttacattatt
    22261 tataaattgc ttatctttat cagaataaaa aataatgcta taagtagatc cttgggtttc
    22321 accattaaat aaatattttt tatttgtgca acctaataag gtaaaaataa gtataagaaa
    22381 taaaataaat tttttattat tcattttttt tacaaatttg caaaaatttt ataattttga
    22441 taaaaatata aaactttaaa ttaaaaatat atgaaaaaag taaaattgtt aataatctta
    22501 ttgattgcat tgataagttt ttcgtgtgat caagataagg agacagaagt acctgagcca
    22561 aaaggtattt tttttagagg tactatagac ggtcataaaa gaactatagc agatggagtt
    22621 aatggatatg tagttaaata tcatgatact tgctatgtca tagattcatt taattattat
    22681 gaacctttta tgattttatc tcaggagtct caaaattatt atgtatcttc aaaagaagct
    22741 attagcttca catttaaaga tttattttta tttcatgatt ctatcactaa taaagattct
    22801 atgctgggag catacttgta taatcataaa ttgattccaa tatattcgaa tgcttcttct
    22861 aacggatctc ttatcatggg tggtttggat attatgtggc gagatgctga gggtagagaa
    22921 tggtttactg gtttgggggc acaaaattct atagctacta tagatacatt agttatagtt
    22981 cgtaagcctg gtatgatcag tgaatataat atgtatatca cttttgactg taaattatat
    23041 accttaggag cttctattag caaagagata aaagatgccc atgctcgtat ttcgattaaa
    23101 aatacttgtt tttattgatt ttttacagtc aattttacta aattttctac atgtgctgtt
    23161 tgtggaaaca tgtcataggg ttgtatgaaa tttattgtat aatcatctat aaacaaagat
    23221 atatcccttg cttgagtagc agcattacaa ctgagataaa ttattgttct aggctttaga
    23281 tttttaataa tattaattac atctttgctc aaaccacaac gaggtggatc gagaaaaatg
    23341 atattgggtt tgtcgatatg ggtgttttca agtattttac tcacgtcacc tttgataaat
    23401 ttgacatttt ttatactatt gattttagca tttttgtttg ctaattgtac ggcttccgca
    23461 ttagattcta tgccgatcac attttttgca atattagcag catatagcga tatagtacct
    23521 gtgccacaat ataagtccca agctacatca tcactattca tatcagatat ttcaatcatt
    23581 tttttataaa tattctctga ttgttcagga ttaatttgaa aaaatgaaaa tgggcttata
    23641 tgaaaagtaa aagaatctaa tatttcactt aggtagcttt taccatgcaa atttacagcc
    23701 attaatccat cgatagaatc atttaattta ttactaatag cataattcca agatatattt
    23761 ggaaaaaaat ctattaattt attaaaaaga ttttctactt tttttgtaaa ttctgatatt
    23821 acaattataa ccatccagtc tcctactcta ttatttctaa aaatcaatga acgtagtgtg
    23881 ccctcatgag tttttttatt ataaaaaggt atgttatcgg ctatgctaaa ctcaaaaatt
    23941 ttatttctta cttcattatg ataaaaatcc atcagatgac attcatctac atgaaaaatt
    24001 ttatcaaatc tacctggtat atgataacct aaggctcgca tatctatatt tatatttttc
    24061 tcatcttcat aaatccatct tttatttgta aaagaatatt ctaatttgtt tctatagtaa
    24121 aaaatttttt tgctaggtat aacctcagga atacaatttt ttagatgtaa gatctttaaa
    24181 aaagcatctt ctacttgagc tttttttgct tttaattgat gtttataatc tatatgttgc
    24241 catttacatc caccacaaat gttgtaatgc tggcatatag gttgcactcg atatgagcta
    24301 gatttatgga tcttaatagg tatagcctcg aaataatttt tataatcttt aataattctt
    24361 acatctatta tgtcgccagg agctacttta ggtactagtc ctactttacc ttcatgcact
    24421 attaagcatt taccttctga agcaaattta tctacgtgta tttgataaat atatttattg
    24481 ttttttgaca tgaaaattta tattagcgat gtgagtttat tttgaaattc tataggattc
    24541 agattataaa ataatttacc ttgatgagcg atcgagatag ctccattttg ttcgcttacc
    24601 actattatca cagcatctga ttgctcacta agtcctatag cagccctgtg acgtgtacct
    24661 aatgaaggat aaagatcatt aaatttactc tcttcggcaa gaggaaatat agttctagca
    24721 gctatgatag tatctttgcg aatgatcatt ccaccatcgt gcaggggtgt atttttgtaa
    24781 aaaatagttt ctatcaattt tctattaact tctgcattta ttattatgcc tgtagctata
    24841 atgtcttgca aagtaatatt tttttcaatt gctattaagg caccaaatcc ttcgtgtgat
    24901 agattgagtg cagcttctat aatttcatta gttttttcac tggtatcata actgggtaat
    24961 ttgaaaaaca aaaagcgttt tctatcattt tgttgaaaaa tcctagtttt ttgtaatggt
    25021 gtacctatta atgataaaaa tcgcttgatt tcattttgaa aaactataat tagcattata
    25081 atacccgcac taataaattg ccccaaaaaa gcactaaaca taggtaaatg aaaaatatta
    25141 accacttgat atagcactac tattaaaata attcctaaaa acactgtaaa tgctatagtg
    25201 ccacgtatga gcatgtaaaa ttcataaata atccatgcta ctaatataat atctattaaa
    25261 tctgtaatgc taaggtgtag tagctgtcct aaaatattcc acactttttt tttagcaaaa
    25321 ttgcaaaaaa aaattcaatt tttatttata attttgtcaa aaatagattt aatgttttta
    25381 tattaattat taatttgagg ttaatttatt tgaggggttg ttagctcagc tggtttagag
    25441 cgcatgcttg acaggcatga ggtcagcagt tcgaatctgc tacaacccac tatttttaac
    25501 taaaaaaata aactacaatc aaaaaaatta attacaaaaa attagatacg ttgaaaatat
    25561 ttatttcttt tcattacagt atatttgcaa gatttaatgc ctgtgtagac taatcatgga
    25621 tccagcgctc tttccaggtt ttgtatttta atttcaattc attttcccag atagggtcta
    25681 ctagttttct atattcttta gtataattat cca
//
LOCUS       DAOPRN010000027        24905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1644_length_24905_cov_125.327163, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000027 DAOPRN010000000
VERSION     DAOPRN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24905
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_24905_cov_125.327163"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKI65_04570"
     CDS             complement(<1..313)
                     /locus_tag="PKI65_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77747.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RC"
     gene            complement(909..1811)
                     /locus_tag="PKI65_04575"
     CDS             complement(909..1811)
                     /locus_tag="PKI65_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOB77748.1"
                     /translation="MIKVQEITKLYGKQKALDKVSFEIKDKTIVGVLGPNGAGKTTLL
                     RILTGYMPPSNGKAQIMDYDINNDIIKIQQNLGYLPEHNPLYLSMYIIEFLNFVANIY
                     KINNKNERIREVIEMVGLKNEINKKIGQLSKGYRQRVGLAKVILHDPPILILDEPTSG
                     LDPNQIIEIRDLIKTLGKKKTVLISTHIMQEVEYLCDRVIILDKGKIVADAPTKDIGV
                     YLSDQIKIEVRFDKKVDTNFFAHLHGIISYQAMPNNSYEILAQYNAKVNEQIFEKAVD
                     HNLKILELKMDQLSVEDVFHHLTQ"
     gene            2100..4277
                     /locus_tag="PKI65_04580"
     CDS             2100..4277
                     /locus_tag="PKI65_04580"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOB77749.1"
                     /translation="MKIKLLIKTLILSILLIPNLLVADEGMWLLNLIEELNYTDMKNK
                     GLRITAEQIYAINKSSIKDAIVMINDGLCTGYMVSDQGLMMTNHHCILEYITDNSIKY
                     NTDFVKNGFYATDMLQELYNPNLRIKFLLKIEDVTDKALKNVTYEMDAIDRQSIINEN
                     IKKIIEEATKGTSYVASVASFYKGSQYILMLYEEYKDVRLVCSPPLAIGFFGGDTDNW
                     MWPRHAGDFAFLRIYMGPDGKPATYNKRKNVPYTPKYFLPISNKGYKEGDFTMSIGFP
                     GNTDRFMTSYGVQQVLDLINPTIVRIRDIKLSILKRNMQADSIIDLMYKSKYNIISNY
                     WKYYMGQIEVMNRLKVMETKRALENDFTKWINEDIQRKNEYRNVLLNIQKIISESNQL
                     RKAVIYYNEAGFNGPEIISFILKFDSLYNIMNNNNLPNELKKSMITDQATKLKYFASN
                     YFFPSYDIKVDKELFITLFQMMYNNLLQQYHPDIFTELSKNYTFALFADELFNNSIFT
                     SKDKLYNFLNNPLLSTLENDLGFKVTQSLYSKYKEIYKSYYNTTISLNNEERKFLAGI
                     REMLKNTYIFYPDANSTMRLTYGKIANYTPIGGLPLTFFTTLDDMIARENPNDPDFII
                     DPHLKKLYNQKDFGIYAVDGKLPLCFISDLDVIGGCSGAPILNGDGEIIGIVFDINWE
                     ATSSQYTFDKEQTRCIATDIRFVLFYLDKYLNAKNIINELKIV"
     gene            complement(4803..5060)
                     /locus_tag="PKI65_04585"
     CDS             complement(4803..5060)
                     /locus_tag="PKI65_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77750.1"
                     /translation="MKDINKYENFTLLAIFSYPHEYFVLKSLLEAEGIEVILKDDITI
                     YVDPLLSQAIGGIKMYVPIDKYKKAKKIFDEYFNENKSQES"
     gene            complement(5179..5835)
                     /locus_tag="PKI65_04590"
     CDS             complement(5179..5835)
                     /locus_tag="PKI65_04590"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HOB77751.1"
                     /translation="MKRMKNLLAIVIIGSVLFVSCNDEDDKAPVVRLIGAADTTIILN
                     STWTDPGAIAEDDKDGVITNIQVSGTVNVDLVGVYKITYSATDAAGNTGTKTRTVRVR
                     NTADGWAGNYSVIDEVEGDQYTYSDIITFSQTENRHVWVTKFAYYTNGAVKFKFEDPE
                     TVPTDVILPQQEVNCGSPAAVRTFSGSGKASTNNSFYIDYIESTYGTSVTGKETYTKQ
                     "
     gene            complement(5902..6438)
                     /locus_tag="PKI65_04595"
     CDS             complement(5902..6438)
                     /locus_tag="PKI65_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOB77752.1"
                     /translation="MKNSFFTSKSSIIRAIIALIIGILAIALPSITLEYLIITIGLLL
                     LLGCVLFIIMAIRTQIKNERNILIVQSIINLVLGLLFIFFPNPVASIFIILLSIILII
                     LGVFLLSSAFFIRRLFRWPWFTLIISILVIISGIVMLINPFDTARAVLIFLGIILLIY
                     GAGELYNAWIFKKSKKNK"
     gene            complement(6845..8086)
                     /locus_tag="PKI65_04600"
     CDS             complement(6845..8086)
                     /locus_tag="PKI65_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOB77753.1"
                     /translation="MFRIKMVELTAPVGTWASLRAAIDNGADSVYLGVGDLNMRSSSA
                     LNFSIKDLPNLVNICHENGVKIYVTLNTVVFDDELEKIDYILDELIKNGVDAVIASDL
                     SVILKAAKRGLPIHISTQANITNIEAIKFYSKWADTVVLSRELTLERIKILSEKIKVL
                     NIRGPNGHLIRLEAFVHGALCMAISGKCYLSLDLEGKSANRGECHQICRRKYKLYNAQ
                     TNQELLIDREHIMSTKDLCTLPFIDKLIDCGVSVFKIEGRGRQPEYVALTTRIYKEAI
                     QSVYDDSFSQQKIEKWMEQLNRVYNRGFWEGYYLGKSLGEWTDQEGSSATEVKEYVGF
                     VSNYFNNIGVAEINLDAGILNLNDNIYIIGPTTGVVECVISEMRNNDNNSIQVSDKGI
                     ITLKVPKKVRKNDKVYLIKKR"
     gene            complement(8158..8949)
                     /locus_tag="PKI65_04605"
     CDS             complement(8158..8949)
                     /locus_tag="PKI65_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOB77754.1"
                     /translation="MLLNYKYIKSRIKTDKYLLILHGLLGCADNWLPQAKIFSKYYNV
                     IIPDLRNHGDSSHHPIMTYEAMSQDVIELLDHLKIDHTSIIGHSMGGKLVMLLALLYP
                     DRVDKIIIVDIAPDKYKIDSNYKLLKILKSIQLNEFKNREEIKNHIINKIPNFKELDL
                     VMKNIRLNLDKKYEWKLNLDALMNNIDNLANFPEIDKVSSTNTLFLRGDKSDHILPKH
                     YDKIYNFFPNAIIQTISNAGHWVQVDNFNDFIVHSINYLQQTTNN"
     gene            complement(9070..9786)
                     /locus_tag="PKI65_04610"
     CDS             complement(9070..9786)
                     /locus_tag="PKI65_04610"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOB77755.1"
                     /translation="MARLSVNINKVATLRNARGGNIPDVTIAAINVERFGAHGVTVHP
                     RPDERHIKYTDVYSIKKVINIEFNIEGYPNDRFLKLVKDVRPTQVTLVPDPPDALTSN
                     AGWDTFKHFDFLSEVIKELKDCGIRTSIFVETDLENIKNAAKTGTDRVELYTEPYAKN
                     FNLNRQQAIKPFIAAANVANQVGLGINAGHDLNLDNLRYFSENIPNLLEVSIGHALIS
                     DSLYFGLENTIQMYLNELGY"
     gene            10023..10853
                     /gene="panC"
                     /locus_tag="PKI65_04615"
     CDS             10023..10853
                     /gene="panC"
                     /locus_tag="PKI65_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOB77756.1"
                     /translation="MKIFNKIKPLQEYLAKQQRPIGLVPTMGALHQGHLSLIEQSKKE
                     NSISVVSIFVNPLQFNDKNDFDKYPRIIDNDLKLLEEYKCDVVLAPSVDEMYPEPPQE
                     HYDFGYLDQTLEGAYRPGHFEGVAIVVNRLFHIVKPDRAYFGKKDFQQYVIIKELVKQ
                     QNLPIEIVAVPIVRESSGLAMSSRNLRLNEQELKVAPEIYKALIKAKSLAYKKSINEI
                     KQIVINHLTQFPNYKIEYVEIVNEENLKPLNDWNEGKAIILIATWLGDVRLIDNMELS
                     "
     gene            complement(10914..13787)
                     /locus_tag="PKI65_04620"
     CDS             complement(10914..13787)
                     /locus_tag="PKI65_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOB77757.1"
                     /translation="MKKWICFILIFVLTNGILCSQINWDDELANAYADYYLSELSWDE
                     KIAQLIFQGIKLDNNNNVFFQKLEKQIDTLCPGGIILLKMTTNDYIKLRNILDKCNKP
                     PTIFSMDGEWGLGMRFTNIKPLPRIMTLSATGDDSLFFEYGKMVGKHFALASIPINYT
                     PILDINDEPLNPVIGTRAISDDPIEVIRRAELFINGLNEYKVLPVVKHFPGHGNTNID
                     SHYNLPFLNMPIEKFKSTHLLPFKYFADYEIPFIMVGHLSFPTYTGDSLPATVNPIIT
                     KKLLKDSLNFRGLVFSDALNMQAIATLAYDSLIIKALNSGIDILLLPENPYKIVSIVK
                     KAVREKKLDSLMIESKVKKILIAKYFIIRDFPLDNYKHYLSDKYIKFYNDEIDSLSEQ
                     IYSKALTLMVDNNAIPLRYDYGKIAMLNLVNNLPKYMDNIWSTYFMYDNYSIKDTALL
                     RQNLNKYDYVFVNLFVSSMHTMNGRDNETIKWLEGISKKHYLILNVIGQPFILAKFND
                     VEDFLAITTTYDIDSIAVDLLAKKLIGFSSFSGHLPIKINEIFYKGMGETSEMLSDVI
                     RFTKPPIGNIEEVDDIMQQALIDSIFPGAVILTIKDNEILYYKTFGTTSYDSSKTKVN
                     RYTIYDLASLTKPLATTLAIMKLYENNKIDLQASISKYLPELRSSSIGKIKIDRIMTH
                     TAGLPPFINFYELFSQDKYPDLYSKTSKADYHQVADSIYVSKSIKDSVINYLKNNIIP
                     NPNSGMVYSDLGFILLKFVVENITKMSFDQYLYNTFYQPLGIKRLCFLPLEKFDKNEI
                     APSEYDSTFRKQLLCGYVHDGNAALLGGISGHAGLFGNAESIAIILQMLLNGGEYGGE
                     AYLKPETIQKFTSTYFDKNRRALGFDKPVINDSDSPACHQASPSSYGHYGFTGTMFWV
                     DPEYKLIYIFLSNRVYPTAKNTKISENSIRTRVQEAIYNYILY"
     gene            complement(13956..14258)
                     /locus_tag="PKI65_04625"
     CDS             complement(13956..14258)
                     /locus_tag="PKI65_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOB77758.1"
                     /translation="MNKPLKTKNMNKEELITAMAKESGLTKADARKALDAFMNVVKMS
                     MARQEKVALVGFGSFEVVQRKEREGRDPRTGKPIKIPAKKVVKFKPGSTLSEAVQK"
     gene            14448..15245
                     /locus_tag="PKI65_04630"
     CDS             14448..15245
                     /locus_tag="PKI65_04630"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOB77759.1"
                     /translation="MKDIISKAFENRELLDDNDVKKSIYNVIDQLDKGIIRVAEPQGE
                     EWVVNEWAKKAILLYFLIQKVEKIEIGPFVFNDKIPLKRDYDINNIRVVPHGIARYGS
                     YIAPGVVMMPSYVNIGAYIDTGTMIDTWATVGSCAQIGKNVHISGGVGIGGVLEPIQA
                     NPVIIEDDCFIGSRSIIVEGVRICRESVIGAGTIITASTHIIDVTKSNPIEYKGFIPP
                     RSIVISGTYSKNFLAGTFNIPCALIIGERNKSTDFKTQLNDILRNNI"
     gene            15317..16798
                     /locus_tag="PKI65_04635"
     CDS             15317..16798
                     /locus_tag="PKI65_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77760.1"
                     /translation="MFRKIVNVVAIVFFVAIWLILIYFKSNYEHSTFQILKNDKTEKF
                     KIAQSSPIFYDSTTNQIYGFCKEIEKNSIDCFFIISLFDHKIDFFDLPANYQNVLGYS
                     KNNKEIVWMSHNDSLSSLLEIQRAKSNISVYYDSILLDINLVIGFGFKNNKPEIALRN
                     PIKNNVIIYTFYDTIFDVRIVRFPIFYQSFSIPNIGYVDKNNWQFLCKALYNEQQYYW
                     RRYDTTQNMNIIRFQENISLPFFYDLLPYPYHTPISSYHYLFFQYLPASHLMFRSDTF
                     LYVPHSTPSQNIIDINTKAIWSILDDTVSVNFFRYDLLLNELSLRKSYEGDKVYYYSK
                     DDKNFDNNLFFVPINNNFSGYINLNNDSIFLLTEDFHYLYINADGISYISYNLINHIV
                     LTINEKLPQKYKILDGYIPNIKAMGIYTMLIGPFVLWIIVWIIVWLINLFSKEKNNYK
                     YYTYHRRTKKKHTITYYTFIGSIIYIIAVFILFQNFLQVFNIL"
     gene            16912..17703
                     /locus_tag="PKI65_04640"
     CDS             16912..17703
                     /locus_tag="PKI65_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HOB77761.1"
                     /translation="MKKRKINVTVIILCCTLLISCNQRNIESNEPNNDHLIMAILYQQ
                     RSAEYEAICYQSFNLAKQIIDKKIQENKNNKKLAIITDIDETILDNSPYEASLVLNKT
                     SYPVKWKDWTSLAKAKPIPGSQDFLNYAASKGITIFYITNRDTDEKGATIENLKKYDF
                     PFADSIHVMTRDSVSSKEIRRNKVRENYEVILYLGDNLADFNDSYLKTTMDQRHQSAY
                     EQRDSFGTIYIVFPNAMYGEWESAMYNYNYKQNTDSIRRSLLISF"
     gene            17807..20035
                     /locus_tag="PKI65_04645"
     CDS             17807..20035
                     /locus_tag="PKI65_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOB77762.1"
                     /translation="MEWYYTFLVIVLLITAVLDLTVGVANDSVNFLGPAYGTRVANFK
                     YIVLIASLGLVIGAVFGGQMMGVARKGIFNPQMFTFHQVMIVFVVAMMSDIILLNIFN
                     AYGLPTSTTVSMVFNLLGAAFGVAIGFILQNHLPFSTVDSFINVSSSLRIITGIFVSI
                     VFAFIFGYLIQLIARIIFSYNYLQVNRIIRTLFNSACIVVITYFIFLKGIKALPFLSE
                     EHYIVIYDNLSLILTCVGIISFVILYMVETIKKYNSFNISILYGTFALAMAFASNDLV
                     NFIGIPLGGLASYQIFSAVGSGADPNTFTMEALLEDPQGSSLLYLLIAGLIMCIVLAR
                     NKKLKTVVETSVRLSQQSETTIERFSVNPIGRMLVDLGYRIGKIFSTSKQTKFSLWLN
                     SRFDIQNIEDKNIAFDKVRATVNLMVASSLIALGTSLTLPLSTTYVTFIVAMGSAFAD
                     KAWGRDTAVYRVSGVVTVIGGWFLTAFLAFLFASLLGFFAYFTDFIGVLIIIAISTLV
                     WIRANRKHSQIASKIKKTDSISHIASKTEVNIQVNNYEKLVFQLLKETTNTIIESLKK
                     EKISVLKDTLKNYEKLKSEVSDFKILLPDIMSQIKGLSTEEYQYFLVKIDLLDQILDI
                     IYYVLNPTLIHLQNHHKPLTQFQIDQIKYIFDIIDNICDIIIDKNKELSNEIIHLLEM
                     EIVKVRRNLIAVSLSDDKNIKMPRRATILVYNILNECDNLVRLLYSMVENNFKKPSFI
                     LT"
     gene            20450..21283
                     /locus_tag="PKI65_04650"
     CDS             20450..21283
                     /locus_tag="PKI65_04650"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOB77763.1"
                     /translation="MTNIFLLTQFLTAQNNGNAINCSNKTISLLGGAYIGISIYTYYS
                     WYYQYNTGKFHFFDDSREWLGMDKIGHFFSANILTKLNKDLLGDCRQSKVLAPLITFG
                     YMTSIEIMDGYSEGWGFSISDMLTNIAGIMTAILLEDQVQWKFSFHSTDWANKRPEIL
                     GENIGQQILKDYNGQTYWLSLSLNKIYAQLPKFLMLSLGYGADAMIYGNPNEQKQVNP
                     SLIPYRQFYFSLDIDWWYLIDKASIRKWKYIFYPLKYIKTPMPTIEISKNKVKLHPFY
                     F"
     gene            complement(21475..22140)
                     /gene="rpe"
                     /locus_tag="PKI65_04655"
     CDS             complement(21475..22140)
                     /gene="rpe"
                     /locus_tag="PKI65_04655"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOB77764.1"
                     /translation="MIHNQVLVAPSLLSANFLDLRKEVELINISEADYLHIDVMDGHF
                     VPNISIGFPLIEQVATISQKPLDVHLMVEKPEQYFDFLFEIGVEMISIHVEDNIHHHL
                     LVQKLQHNNVKAGLVLNPFTPVELIYDNLPYIDYVLIMSVNPGFGGQKFLPFSINKVQ
                     KLHDFIEKEGLNCLIEIDGGINLENAKKLIASGVDILVAGTSIFKAHNPLEVIKEMKK
                     LAK"
     gene            complement(22176..23852)
                     /locus_tag="PKI65_04660"
     CDS             complement(22176..23852)
                     /locus_tag="PKI65_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77765.1"
                     /translation="MKKNNFYIFILILILPIANFAQKTDTTKKYDEKVIVIGEYKLEL
                     PNIAKPILQPTIYEEKAEKHSLQYDIFPHPIFITYNPSPLKPIKLDKEPIEALPSNYA
                     KFGVGNKMIDWHLSIANSIKTKRNYGIYYDGFGLYSKISNYGPPAHWKHNLNGYIEWI
                     QNRFTLTLSAGYDASIIHYYGFKKTEYPNDSLTKKDHLQKYNTININFDVVSNSTNPN
                     EFKQEYKISGTILPTYLKNNEYRLGALVNIYQNVKWLPLKNVTQTPAMYLKYNHYFEN
                     NTLEKYNFGIFNSLLSYQLKEKIWALKLGVSIDVIADSVGSARVFPYIGGKLIFVPEI
                     FELHASFGSFTEVYSLDSLYHINPFIHQFIKPTPVVWRQYFTGSLVSKLFNKLQLEIG
                     VSYGKTNEYAFFINDTIIRPYSRFNVVYDTTTALIPFLNIKWYSDRLNINWQTRYNHL
                     ETKSEAKPWHEPNFVSTLDVKYLFAKKFLVGTNLTFVGKRYACQLKPDNNDTIELSPF
                     VDWNLGIEYFFNNSLSFYLNIYNILSKPQYYYWHLPTYKVHFLLGITYNF"
     gene            complement(24215..>24905)
                     /locus_tag="PKI65_04665"
     CDS             complement(24215..>24905)
                     /locus_tag="PKI65_04665"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77766.1"
                     /translation="CMLEGKTLRACAKEVGISLPTSFAWRHRILAALRNFEANVNFFG
                     IVEVEELLMNYSEKGRRYRNEEELAEAREKKKKKVAVIASKDRTGNMLFKQLGLGKIK
                     REQIEQLLNTKVSANSVICFGHNDEFRKLKSQNLERKEIVIDRVRKRGVYSVATIRRH
                     VNTFVGWINNQFRGVATKYLQSYIMWYVVMHKYLLNNVVPDVGRMLNLASSDRRAWYV
                     YRDLRASPMYI"
BASE COUNT         8857 a         3439 c         3402 g         9207 t
ORIGIN      
        1 tacatctgat gtaatcgagc attttctctt tcttttgcaa cctgtaaaca aaagttccag
       61 tggtaactct aaattgtctg ccgcagtctt tgcagcgata gttttgcatt ccattttgta
      121 agccattttt aataatgtga ttgctacgac agtctgggca tagtggcggg gcactgagta
      181 ttttcaggcc ttgataatcg actacatcgg cttctaattt tttcattaga gaataaagat
      241 tgtctaagaa ttctttattc tcttccttgc tcatagcaag gtatttactg gtgttttcta
      301 ctattttttt catgaattaa aatttttgta aaaatataaa aattatttaa catataaact
      361 tgaaaaactt aaacatttat tagtttcatt catcataacg ttagaattac taacttatac
      421 aactccccct atctcatgct tttcgatatt atattattaa ataccaaatt taatgtacct
      481 ataatattaa tgtggttcta taaagtttat tgtgggtaaa aacattttag attattacaa
      541 aaaataacta tctttaatta tttaggttga tttttttacc acaaagggca ctaagttatt
      601 acactaagtc cacaaagtgc catttataac ccttttaatt tcatctttct ttgtgttctt
      661 tgtgcattct ttgagaacgt tgtggttaat atttaacagt cttataatta ataaaccaat
      721 tattaacaat ttttttataa aatcactctt ttttaatctt tacccataca aaacgttata
      781 taactattaa tgttttttat ataaaaaaat gttatagtat tccttatatt aaaaaaatga
      841 ttctacaata catgctataa aaaattagat tgaaaaaata agaataaagt ttaacaaatc
      901 tataatgatc attgagtgag atgatgaaaa acatcttcta cactgagctg gtccattttc
      961 agttctaaaa tttttaaatt atgatctaca gctttttcaa agatttgctc atttaccttt
     1021 gcattgtatt gagctaatat ttcataacta ttatttggca tggcttgata tgagatgatg
     1081 ccatgtaagt gagcaaaaaa attagtatct acttttttat cgaaacgaac ttctattttt
     1141 atctgatcag atagataaac acctatatct ttagtgggtg catctgctac tattttccct
     1201 ttatccaata ttattactct atcacataag tattctactt cctgcataat atgagtagat
     1261 atcaaaacag ttttcttttt tccaagtgtt ttaataagat ctcttatttc gattatttga
     1321 tttggatcta aacccgaggt gggttcatct aagataagaa taggaggatc gtgcaaaatc
     1381 acttttgcta aacctacacg ttgacgatat cctttagata attgtcctat ttttttattt
     1441 atttcgtttt ttaaaccaac catttctatt acttcgcgta ttctttcatt tttgttattt
     1501 attttgtata tgtttgctac aaaatttaaa aattctatta tatacatcga tagatacaag
     1561 gggttgtgtt cgggtaaata acctagattt tgttgaattt ttataatatc attattaata
     1621 tcataatcca ttatttgtgc tttgccattt gatggtggca tatatccagt cagtattcgt
     1681 agtaaagtgg ttttgccagc accatttggt cctaaaacac ctacaattgt tttatccttt
     1741 atttcaaatg atactttatc taaagctttt tgttttccat atagtttagt gatttcttgt
     1801 acttttatca taatttattt ttaaatgttt atcaattttt atacaaaatt aaaaaaatta
     1861 tcatagtatt tttaattata ttttaatatt gactatattt atttattaaa ttgaaaattt
     1921 ataatctcaa aatataaatg agatgatagc tgagagaaaa aaggtaaaga agtgagtgag
     1981 actggaaact tggaggcaag aagaagagat attgttagtt attatttgtt taatgtaatt
     2041 taaaatttaa ttatatattt tatttatttt ttttatatat ttgcagaaaa ttaaaagcta
     2101 tgaaaataaa attattgata aagactttaa ttttaagtat attattaata ccaaatttat
     2161 tagttgcaga tgaagggatg tggcttttaa atttaataga ggagctcaac tacactgata
     2221 tgaaaaacaa aggacttaga ataactgccg agcaaatata tgctattaat aaaagttcca
     2281 ttaaagatgc tatagttatg ataaatgacg gactttgtac tgggtatatg gtatctgatc
     2341 agggattgat gatgactaat caccattgta ttttagagta tattactgat aattctataa
     2401 aatataatac tgattttgtg aaaaatggct tttatgctac tgatatgcta caagagcttt
     2461 ataatccaaa cttaagaata aaatttttac ttaaaataga agatgtaaca gacaaagcat
     2521 tgaaaaatgt aacttatgaa atggatgcta tagatagaca atctataata aatgaaaata
     2581 ttaaaaaaat aatagaagaa gccactaaag gtacttcgta tgtagcttca gttgcatctt
     2641 tttataaagg ttcacaatac attctgatgt tatatgagga atataaagac gttagattag
     2701 tatgttcgcc gccattagct attggatttt ttggtggcga tacagataac tggatgtggc
     2761 ccagacatgc aggtgatttt gcatttctac gtatttatat gggaccagat gggaaaccag
     2821 ctacttataa taaaagaaaa aatgtgcctt atactcctaa gtatttctta ccaatttcta
     2881 ataaaggata taaagaaggt gatttcacta tgagtatagg ttttccagga aatactgata
     2941 gatttatgac ttcatatggt gtgcaacaag tccttgatct tataaatcct actatcgttc
     3001 gcattagaga tattaaatta agtattctaa aaagaaatat gcaagccgat tctatcatag
     3061 atctaatgta taaatctaaa tataatatta tatctaatta ttggaaatat tatatgggac
     3121 agatagaagt aatgaacaga ctaaaagtaa tggaaactaa acgcgctctc gaaaatgatt
     3181 ttactaaatg gataaatgaa gatatacaac gtaaaaatga atatagaaat gtattattaa
     3241 atattcagaa aatcatctca gagtcaaatc aattaagaaa agctgttata tactataatg
     3301 aagcaggatt taatggtcct gaaatcatct cttttatttt aaaatttgat agcttataca
     3361 atattatgaa taataataat ttacctaatg aactaaaaaa atcaatgatt acagaccagg
     3421 caactaaatt gaaatatttt gcttctaatt attttttccc tagttatgat attaaagttg
     3481 ataaagagtt atttatcaca ttatttcaaa tgatgtataa taatcttcta cagcaatatc
     3541 atcctgatat ttttacagaa cttagtaaaa actatacttt tgctttattt gcagatgagt
     3601 tatttaataa ttctattttt actagcaaag ataaacttta taattttcta aataatcctc
     3661 tattatctac attagaaaat gacttgggat ttaaagtcac acagtcatta tattctaaat
     3721 acaaagaaat atataaaagt tattataata caactatttc attaaataat gaagagagaa
     3781 aatttttagc tggaatacgt gaaatgttaa aaaatacata tatattttat cctgatgcta
     3841 atagcactat gagacttacc tatggtaaga ttgcaaatta tactcccata ggtggactcc
     3901 cgctaacttt ttttacgact ttagatgata tgatagcacg cgaaaatcct aatgatccag
     3961 acttcattat agatccacat ttaaaaaaat tgtataatca aaaagatttt ggtatttatg
     4021 cagtggatgg taagttgcct ctatgtttta ttagtgattt agatgttatt ggtgggtgct
     4081 caggtgcacc aatattaaat ggtgatggag aaatcatagg tatagttttt gatattaatt
     4141 gggaagcaac atcatcacaa tatacttttg acaaagaaca aacaagatgt atagctactg
     4201 atatcagatt tgttttattt tacttagata aatatttgaa tgcaaaaaat atcataaatg
     4261 aactaaaaat tgtgtaattg taataaaaca taaatttttc aattactaaa actgatccct
     4321 gataaaatat tattcaattt agtaatactt cagataaatt ttgagatttt agtagatcct
     4381 actcaaaaat atttagacaa taagtatata taattaaata cttaagtttt tttaatatcg
     4441 atcaagttat atagatacta aaaaattggt tctataacgt ttattgtggg taataaaatt
     4501 ttatattgtt acaaaaaaat aattattttt taattattta ggttgatttt ttttaccaca
     4561 gagaacacca agttattaca caaagtccac aaagtgccat ttatagtcct tttaatttca
     4621 ttcttctctg tgttctttgt gcattctttg agaactttgt ggttaatatt taactacact
     4681 ataattaata aactaatatt aacaaatttt ttataaaatt actctttttt taatatttac
     4741 ccacacaaaa cgttatagat actaaaaaat tattaaatag tatagggtgt tttttcaaac
     4801 tttcaacttt cttgtgattt attttcatta aagtattcat caaaaatttt ttttgctttt
     4861 ttatatttat ctataggtac atacattttg atgccaccta tagcttgaga cagtaaagga
     4921 tccacataaa tggtaatgtc atcttttaat attacttcta taccttcagc ttctaacaaa
     4981 gattttagca caaaatattc gtgaggataa gaaaaaatag ctaataaagt gaaattttca
     5041 tatttattga tatctttcat ttatcaatat ttttaataaa attacaataa ataatgaaat
     5101 aaagcaacat tttattataa aaaaagagag gtaaaaaatt ttacctctct ttttttttta
     5161 taaacttatt taaacaattt attgtttggt atatgtttct ttgccagtaa cactagtacc
     5221 ataagtgctt tcaatataat caatatagaa agaattgttt gttgatgctt taccagaacc
     5281 agagaaagta cgaactgcag ctggactacc acaattcact tcttgttgtg gaagaattac
     5341 atctgttggc acagtttctg gatcttcaaa tttgaattta actgcaccat tagtataata
     5401 agcaaattta gttacccata catgtctatt ttcagtttga gaaaaagtaa taatatcaga
     5461 ataagtatat tgatctccct ctacttcatc tataacactg tagttaccag cccatccatc
     5521 agcagtattt ctcactctta cagtacgagt ttttgtacct gtattgccag cagcatcagt
     5581 tgctgaatag gtgattttgt atacgccaac taaatctaca tttacggtcc cactaacttg
     5641 aatattagta atcacaccat ctttatcatc ttctgcaatg gcacctgggt ctgtccatgt
     5701 agaatttaat ataatagtag tatctgcagc tcctattaat ctgactactg gggctttgtc
     5761 atcttcgtca ttacaggaaa caaataatac gcttcctatt atgactattg ctaataaatt
     5821 tttcattctt ttcataatct tatttttttt taaggtttga cattacaaaa ttaagtaata
     5881 ttttttaaat aaaaaataaa tttatttatt ttttttactt tttttaaaaa tccatgcatt
     5941 atacaattcg ccagcaccat atattaataa aataattcct aaaaatatta agactgctct
     6001 ggcagtatca aaaggattta ttaacataac aataccagaa ataataacta gtatcgatat
     6061 aattaatgtg aaccaaggcc acctaaataa gcgtcttata aaaaatgcac tagataggag
     6121 aaaaacacct aatattatca agataatact taataaaata ataaaaatac ttgcaacagg
     6181 atttgggaag aaaataaata ataaccctaa aaccagattg ataatagatt gtacaattaa
     6241 tatattgcgt tcatttttaa tttgtgtcct tatagccatt ataataaaca aaacacaacc
     6301 taataataat aacagaccaa tagttataat taaatactct aatgttatac ttggcaatgc
     6361 tattgctaat ataccaataa ttaaagctat aatggctcta attattgaac tttttgatgt
     6421 aaaaaaacta tttttcataa atttttttac aaatttacca aaaataaata aaataaataa
     6481 ttttactttt ttctttgtcc tcggctacat atattactat ctcaaaacat ttgtatttat
     6541 taaaaaatca agtaagaata tttttaaggt tctataacgt ttattttgta ataaaatttt
     6601 agattattac aaaaaaataa ctatttttta attatttagg ttattttttt accacaaagt
     6661 gctatttgta gcccttttaa ttccatactt ttctgtgttc tttgtgcatt ctttgagaac
     6721 tttgtggtta atatttaact gcactataat taataaacta atattaacaa tttttttata
     6781 aaactactct tttttttaat ctttacccat ataaaacgtt atagaatcaa tatttttaat
     6841 aagcttatct ttttttaatt agataaactt tgtcattttt tctaactttt tttggaactt
     6901 ttaaagtaat tataccttta tcagaaactt gaatagaatt attatcgtta tttctcattt
     6961 cgctaattac gcattctaca acacctgtag taggaccaat gatataaata ttgtcattaa
     7021 gattcaaaat accagcatct aaattgattt cagctacacc gatgttatta aaataattag
     7081 aaacgaaacc cacatattct tttacttcgg tagcagagct accttcttga tctgtccatt
     7141 ctcctaaaga ttttcctaaa taataacctt cccagaagcc tctattataa actctattta
     7201 attgctccat ccatttttcg attttttgtt gagagaaact atcatcatag acactttgta
     7261 tagcttcttt ataaatacga gtagttaaag ccacatactc tggctgacgt ccacgacctt
     7321 caattttaaa aaccgaaact ccacaatcaa ttagtttatc tataaatggc aaagtacata
     7381 aatcttttgt agacataata tgttcacgat ctattaataa ttcttgatta gtttgtgcat
     7441 tatataattt atatttacgt ctacatattt gatgacactc acccctattg gcagatttgc
     7501 cttcgagatc taaactcaaa taacatttgc ccgatatagc catacataaa gcgccatgca
     7561 caaaagcttc taaacgaatc aaatgaccat taggacctct aatattcaaa acttttattt
     7621 tttcagataa tatttttatc ctttctaaag tcaactctct agataatact actgtatcag
     7681 cccattttga ataaaatttt atggcttcta tatttgtaat atttgcttgt gtggaaatat
     7741 gaataggcaa tcctcgttta gccgctttta aaatcactga tagatcactt gctatcactg
     7801 catctacacc gtttttgatt aactcatcta aaatgtaatc tattttttct agttcgtcat
     7861 cgaaaactac agtattcaga gtaacatata tctttacgcc attttcatga catatattta
     7921 ctaaatttgg taaatctttt atagaaaaat tcaatgctga ggagcttctc atattcaaat
     7981 cacctactcc taaatacaca ctatcggctc cattgtctat tgcagctctt aagctagccc
     8041 atgtccctac aggtgctgtc aattctacca tttttattct aaacattttt tacaaaatta
     8101 attttaaaaa atcaaaataa cagataaaag ataaaaaatt taaatggagc accattgtca
     8161 gttgttagta gtctgttgta agtaattaat cgaatgcaca ataaaatcat tgaaattatc
     8221 cacttgcacc caatgaccag cattagatat ggtttgtatt attgcatttg ggaaaaaatt
     8281 atatatttta tcgtaatgtt taggtaatat atggtcactt ttgtcgcctc ttagaaatag
     8341 agtattggta cttgacactt tatctatttc tggaaaatta gccaaattat caatattatt
     8401 cattagtgca tctagattaa gtttccactc atatttttta tctaagttta atcttatatt
     8461 tttcataact aaatccaatt ctttaaagtt aggaatctta tttatgatat gattttttat
     8521 ttcttctcta tttttaaatt catttagttg tatacttttt aatattttta atagtttgta
     8581 gtttgaatca attttgtact tatctggagc tatatctacg attatgattt tatctactct
     8641 atcagggtat aaaagagcta atagcatgac taatttgcct cccatactat gacctattat
     8701 agatgtatga tctattttta agtgatcgag tagttctata acatcttgac tcatagcttc
     8761 gtaggtcata ataggatgat gcgaggaatc accatgattt cgcagatcag gtatgatgac
     8821 gttataatat tttgaaaata ttttagcttg cgggagccag ttgtctgcac accctaaaag
     8881 tccgtgtaat attagtaaat atttatcagt tttgatccta gattttatat atttataatt
     8941 taataacatt atcaataata aaatttttag aaactaaaat tttaatcaaa aatgatcatt
     9001 tatggcttta gctaactatt attttttttg catttatgtt agagctttag catccacgct
     9061 attaattgat taatatccaa gttcattcaa atacatttgt atagtgtttt ctaaaccaaa
     9121 atataatgaa tcactaatga gagcatgccc aatgcttact tctaaaagat taggaatatt
     9181 ttcggagaaa taccttaaat tatccaagtt aagatcgtgt cctgcattta tccctagtcc
     9241 aacctgatta gctacattag cagcagcgat aaaaggtttg atagcttgtt gtctattaag
     9301 attaaaattt ttggcatagg gttcagtata caattctact ctatcagtac cagttttagc
     9361 agcatttttt atattttcta aatcagtttc tacgaatata gaagttctaa tcccacaatc
     9421 ttttagttct ttaatcactt cactaaggaa atcaaaatgt ttaaatgtat cccatccagc
     9481 attactagta agagcatctg gagggtctgg caccaaagtt acttgagtag gacgtacatc
     9541 ttttactaat ttcagaaatc tatcattagg atacccttca atgttaaatt caatatttat
     9601 tacttttttt atggaataaa catctgtata ttttatgtgt ctttcatcgg gtctaggatg
     9661 aacagttacg ccatgagcac caaagcgttc gacattaata gctgctatag taacgtctgg
     9721 tatattacca cccctcgcat ttcgtagagt agctacttta ttaatattta cgcttaatct
     9781 agccatttat ttttaattat tttttgcaaa tttacaagta aaattctaat ttataaaaat
     9841 atttaattat aaaatacaat aataaattaa ataaaaatcg ttacagaatt gcattatagc
     9901 aaggtattat catgacatat tttatagttt tctgataaaa agttttaatt tttatatctt
     9961 tttttaatta attttttatc cgctttaatt tttactaatt ttgccaatta aattttttta
    10021 gaatgaaaat atttaataaa attaaaccat tgcaggaata tttagcaaaa cagcaacgcc
    10081 ctattggttt agtacctacc atgggagctt tacatcaagg gcatttatct ttaatagagc
    10141 agtctaagaa agaaaattct atttcagttg ttagcatatt tgtaaatcca ttgcaattta
    10201 acgataaaaa cgattttgat aagtatccac ggataataga taatgatttg aaacttttag
    10261 aggaatataa atgtgatgtg gttttagctc ctagtgttga tgaaatgtac cctgagccac
    10321 cacaagaaca ctatgatttt ggctatttag accagacact tgaaggtgca tataggcctg
    10381 gtcatttcga gggtgtcgcc atagtagtaa atagattatt tcatattgtc aagcctgaca
    10441 gagcttattt tggtaaaaaa gattttcaac aatatgtaat aataaaagag ttagtaaaac
    10501 aacaaaattt acctatagaa atagtagcag taccaatcgt tagagaatca agtggtctag
    10561 cgatgagtag tcgcaatcta aggctaaatg agcaagaact aaaagtggcc cccgaaatat
    10621 ataaagcact tattaaagct aaatcattag cttataaaaa atctataaat gaaataaaac
    10681 aaatagtaat aaatcattta acacaatttc caaattacaa aatagaatat gtagaaatag
    10741 taaatgaaga aaacctaaaa ccacttaatg attggaatga agggaaggct ataatcttaa
    10801 tagctacgtg gttaggtgat gtaagattga tagacaatat ggaattatct taaaatatta
    10861 actagcaagc tatgacttct ctctttatcc ttgttttaat tttttctgtg tatttaatat
    10921 aatatataat tatagatagc ttcttgtact cgtgttctaa tagaattttc tgaaattttt
    10981 gtatttttag ctgtcggata cactctatta gacaaaaaaa tgtatatcaa tttgtactct
    11041 ggatctaccc aaaacatagt acctgtaaac ccataatgac catagctaga tggagaagct
    11101 tggtgacatg cagggctatc actatcatta atgacaggtt tgtcgaagcc taaagctcgt
    11161 ctatttttat caaaataagt agaggtaaat ttttgtattg tttctggctt tagatatgct
    11221 tcaccgccat attcacctcc gtttaatagc atttgtaata ttatagctat agattcggcg
    11281 ttaccaaaca atcctgcatg tccagagatg ccacctaata gtgctgcatt gccatcatgt
    11341 acataaccgc aaagtagttg ctttctaaat gtactgtcat actcgctagg agctatctca
    11401 ttcttatcaa atttttctaa gggtaaaaaa cataaacgct tgattcctaa gggttgataa
    11461 aaagtattat ataaatattg atcgaaactc atttttgtta tattctctac aacaaatttt
    11521 agtaatataa aacctaaatc agaatatacc atgccactat tgggatttgg tataatattg
    11581 ttttttaaat aattaatgac gctgtctttg atagatttac taacataaat actatcagct
    11641 acttgatgat aatctgcttt tgatgtttta gaatacaaat cagggtattt atcttggcta
    11701 aaaagttcgt aaaaattaat aaaaggtggc aaaccagcag tatgagtcat gatgcgatct
    11761 attttaattt ttcctataga actactacgt agctcaggta aatatttaga aattgatgct
    11821 tgcaaatcta ttttattgtt ttcatataat ttcattatag ctagagtagt agccaagggc
    11881 ttagtaagtg atgctaaatc atatatagta taacgattta cttttgtttt tgaagaatca
    11941 taagaggtgg tgccaaatgt tttgtaatat aatatttcat tatctttaat agttaatata
    12001 acagctccgg gaaaaatact gtctattagt gcttgctgca ttatatcatc tacttcttcg
    12061 atatttccta ttgggggctt ggtaaatcta attacatcgg acaacatctc actagtctct
    12121 cccattcctt tataaaatat ttcattgatt ttaataggca aatgccctga gaatgagcta
    12181 aaaccaatta atttttttgc taataaatca acagcaatag aatctatatc ataagtagta
    12241 gtaatagcca aaaaatcttc tacatcatta aatttagcta atataaaagg ttgaccaata
    12301 acatttaaaa tcaagtaatg cttttttgat ataccttcta accatttaat agtttcatta
    12361 tctctaccat tcatagtatg catagagcta acaaataaat ttacaaaaac ataatcatat
    12421 ttattcaagt tttgtctaag cagggctgta tcttttattg aataattatc atacatgaaa
    12481 tatgtagacc agatattatc catatattta ggtaaattat ttactaaatt tagcatagct
    12541 attttcccat aatcatatct gagaggtatg gcattattat ctaccatcaa agttagagct
    12601 ttagaataga tctgttcaga cagactatct atctcatcat tatagaattt gatatattta
    12661 tctgatagat aatgcttata attatcaaga ggaaaatctc taatgatgaa atatttagct
    12721 attaatattt tttttacttt actttctatc ataagggaat ccaatttttt ttctcgtact
    12781 gcttttttga caatagaaac aattttataa ggattttcag gtaataataa tatgtctatt
    12841 ccagaattta atgccttaat gattaaagaa tcataagcta gcgtagctat ggcttgcata
    12901 ttaagggcat cagaaaaaac taagcctcga aaatttaaac tatcttttaa taatttttta
    12961 gttataatag gattgacagt tgccggtaaa ctatcaccgg tatatgtagg aaaactaaga
    13021 tgccctacca taataaaagg aatttcgtaa tcagcaaaat acttaaaagg taataaatgt
    13081 gtagatttga atttttctat gggcatattc agaaaaggaa ggttgtagtg cgaatcgata
    13141 ttagtatttc catgaccagg gaaatgctta actactggta ggactttata ttcatttaaa
    13201 ccatttataa acaattcggc ccttcgtatc acttctattg gatcatcgct aatagcacga
    13261 gtgcctatta ctggatttag tggttcatca ttaatgtcga gaatgggggt ataattgatg
    13321 ggtatagaag ctagagcaaa gtgtttgcca accattttac catattcaaa aaataaacta
    13381 tcatccccgg tggcactcaa agtcatgatt ctaggtagtg gttttatatt agtaaatctc
    13441 atccctaagc cccattctcc gtccattgaa aaaattgttg gaggtttatt acatttatct
    13501 aaaatatttc taagtttaat gtaatcattt gttgtcattt ttaaaagaat aataccacca
    13561 ggacacagtg tatcgatttg tttttctaat ttttgaaaaa atacgttgtt attattatct
    13621 aatttaatac cctgaaatat taattgagct attttctcat cccaagaaag ctcagataaa
    13681 taataatcag cataggcatt agctagctca tcatcccaat ttatctgaga acataaaata
    13741 ccattagtaa gtacaaaaat taaaatgaag catatccact ttttcattat cttttaagaa
    13801 atttattata aaataaagaa ataattaatt tacaaagata aaaaaactta atataaaatt
    13861 aaatgtaatt aagaaaatag aaggcaaatc agtatttatt taaacaaaaa ggctcgcttt
    13921 ataaagcgag cctttttgtt taatttatca ggtttttact tttgtacagc ttcacttaga
    13981 gtagaacctg gtttaaattt cactactttt tttgcaggta tttttattgg tttacctgtt
    14041 ctaggatctc taccttcacg ttcttttctt tgtacgactt cgaaactacc aaagccaact
    14101 aaagcaactt tttcttgtct tgccattgac attttaacaa cattcataaa agcatctaat
    14161 gcttttctgg catcagcttt tgttaatcca ctttcttttg ccattgctgt aattaattct
    14221 tctttattca tattttttgt ttttaggggt ttgtttattc ctgttgcaaa attatgtatt
    14281 ttttttaata tgcaaatata tatgcatttt ttattaaaat aatttataaa aacacataat
    14341 ttattaataa atattattat tatttagcct aaaaatatgt ttatttatac aaattaattt
    14401 ttagtatttt ataaaaaatc atttactttg cgatatatat ttgaaatatg aaagatataa
    14461 tttctaaagc atttgaaaat agagagctct tggatgataa tgatgttaaa aaatcgatat
    14521 ataatgtcat tgatcaacta gataagggaa taataagagt agcagaaccc caaggagaag
    14581 aatgggtcgt aaatgaatgg gctaaaaagg ctatattatt atatttttta atccaaaaag
    14641 ttgaaaaaat agaaataggt ccttttgttt ttaatgataa aataccactc aaacgagatt
    14701 atgatataaa taatataagg gttgtgcctc atggtattgc tagatatggt agttatattg
    14761 cgccaggtgt cgttatgatg ccttcttatg taaatatagg ggcatatata gatactggta
    14821 ccatgataga tacatgggct actgtaggct catgtgctca aattggcaaa aatgtacaca
    14881 taagtggtgg agtaggaata ggtggggtat tagagcctat tcaagccaat ccagtgatca
    14941 ttgaggatga ttgctttata ggttcgcgtt ctataattgt agaaggtgtc agaatatgta
    15001 gagaatcagt aatcggtgca ggtaccatta ttactgcatc tacacatatt attgatgtta
    15061 ctaaatcgaa tcctatagag tataaaggtt ttataccacc gcgaagtatt gtaatatctg
    15121 gcacttattc taaaaatttt ttagctggca cttttaatat tccttgtgca ttgataattg
    15181 gtgagagaaa taaatctaca gattttaaaa cacaattaaa tgatatactt agaaataata
    15241 tttgataatt tataaaaaag gaataatttt tataattttg gattactttg agtttgatat
    15301 ataataatat gaaataatgt ttagaaaaat tgttaatgta gttgctatag tcttttttgt
    15361 agctatatgg ttaatattga tatattttaa atctaattat gaacactcta catttcaaat
    15421 attaaaaaat gataaaacag aaaaattcaa aatagcacaa tcatcaccta ttttttatga
    15481 ttctacaaca aatcaaattt atggtttttg caaggaaatc gaaaaaaaca gcattgattg
    15541 cttctttatt atttcattat ttgatcacaa aatagatttt tttgacttac cagctaatta
    15601 tcaaaatgtt ttaggatatt caaaaaacaa taaagagata gtatggatgt ctcataatga
    15661 cagtctatct tctttactcg aaatacaaag agctaaatct aatatttctg tttattacga
    15721 cagcatccta ttagatataa atttagtaat tggttttgga tttaaaaata ataaacctga
    15781 aatagcatta cgaaatccta taaagaacaa tgttattatt tatacatttt atgatactat
    15841 atttgatgtc aggatagtaa gatttcccat attttatcaa tctttttcta ttccaaacat
    15901 tggatatgta gataaaaata attggcaatt cctttgcaaa gcactatata atgagcagca
    15961 atattattgg aggcgttatg acacaactca aaacatgaat atcattagat ttcaagaaaa
    16021 tatttcttta ccattttttt atgatttact accatatcca taccatacgc caatatctag
    16081 ttatcattat ttattttttc agtatttacc agcttcgcat ttgatgttta gatctgatac
    16141 ttttttatat gtgcctcatt ctacaccatc acaaaatatc attgatataa atacaaaagc
    16201 tatatggagt atattagatg atacagtttc tgtcaatttt ttccggtacg acttactttt
    16261 aaatgaatta tctctgagaa aatcttatga aggtgataaa gtctattatt attctaagga
    16321 tgataaaaat ttcgataata atctgttttt tgtgcctata aataataatt ttagtggcta
    16381 tataaactta aataatgata gtattttttt actaactgaa gattttcatt atttatatat
    16441 aaatgcagat ggtatcagtt atatttcata caatttgatc aatcatatag tattgacaat
    16501 aaatgaaaaa ttacctcaaa agtataagat cctcgatgga tatattccaa atataaaagc
    16561 tatgggtata tatactatgc tgataggtcc ttttgtacta tggattatcg tttggataat
    16621 agtatggtta ataaatttat ttagcaaaga aaaaaataat tacaaatatt atacctatca
    16681 taggcgaact aaaaaaaagc atactattac atattatact ttcattggtt ctattattta
    16741 tattattgca gtatttatac tttttcaaaa ctttttacaa gtgtttaata ttttgtaatt
    16801 gaacaataaa agattttatt tatttttata aaaatttaat aaaaatttaa caatttctta
    16861 acttttatta tgaatcaaat tttataattt taccaaaaaa tataaaaaat aatgaaaaaa
    16921 agaaaaatta atgtaaccgt aattattttg tgttgtacct tattgatttc ttgtaatcaa
    16981 agaaatattg agagcaatga gcccaataat gatcatctga taatggcaat tttataccaa
    17041 cagagatctg ctgaatatga agcgatatgt tatcaaagtt tcaatcttgc taagcagatt
    17101 attgataaaa aaatacaaga gaataaaaat aataaaaaat tagctattat tacagacatt
    17161 gatgaaacga tattggataa tagtccttac gaagcttctt tagttcttaa taaaactagc
    17221 tatcctgtaa aatggaaaga ttggacatca ttagcaaaag caaagccaat tccaggctca
    17281 caagattttt taaattatgc tgcatccaaa ggaattacta tattttacat aactaataga
    17341 gatacagatg aaaaaggtgc tacaatagag aatttaaaaa aatatgattt cccttttgct
    17401 gattctatac atgtgatgac tagagatagt gtttctagta aagaaatcag acgaaacaaa
    17461 gtaagagaaa attatgaagt aatattatat ttaggagata atctcgcaga ttttaatgat
    17521 tcttatctca aaaccactat ggaccaaagg catcagtccg cttatgaaca acgtgactct
    17581 tttggtacta tatatatagt atttcccaat gctatgtatg gagagtggga aagtgctatg
    17641 tataactata actataaaca aaatacagac tctatcagaa ggagtttact tattagtttt
    17701 taaaatataa atagtaattt taaagatgaa atttcaacga ataacttaca acctatcaac
    17761 ttttacactt cttaattttc tatatatcat aattaaaaca taaaatatgg aatggtacta
    17821 cacatttctt gtaatagttt tattaattac agcagtttta gatctaaccg taggtgtagc
    17881 taatgattca gtaaattttt tagggccagc ttatggtact agagtagcta attttaagta
    17941 tatagtatta atagctagtt taggattagt cataggagca gtatttggag gtcagatgat
    18001 gggagtagct agaaaaggta tttttaatcc tcaaatgttt acttttcatc aagtgatgat
    18061 agtttttgtg gtagctatga tgtctgatat tattttatta aacatcttta atgcttatgg
    18121 attgcctaca tctactacgg tttcgatggt ttttaattta ttaggtgccg catttggagt
    18181 agccataggt tttattttac aaaatcattt gccttttagt acagtagata gttttataaa
    18241 tgtatctagc tcacttagaa taattacagg tatttttgtt tctatagttt ttgcatttat
    18301 atttggctac ctaatacaat taatagcacg tataattttt tcttataatt atttacaagt
    18361 aaataggata ataagaacct tatttaattc tgcatgtata gtagtgataa cgtattttat
    18421 ctttttaaaa ggcatcaaag cgcttccatt tttgagtgaa gagcattata tagtgattta
    18481 tgataattta tctttaatat taacatgtgt cggaataata tcatttgtta ttttatatat
    18541 ggtagaaact ataaaaaaat ataatagttt taatattagt atattgtatg gcacttttgc
    18601 cttagctatg gcatttgctt ctaatgattt agtcaatttt attggtattc cattaggagg
    18661 gctagcatcg tatcaaattt tttctgctgt tggcagtggg gcagatccaa ataccttcac
    18721 catggaggct ttattagagg atccacaagg tagttcttta ctgtatttat taatagcggg
    18781 attgataatg tgtatagtat tagcaagaaa taaaaaatta aaaactgtag tagaaacttc
    18841 tgtgaggttg agtcagcaat ctgagactac tattgagaga ttctcagtaa accctatagg
    18901 taggatgcta gtagatctag gctatagaat aggtaaaata ttttctactt ctaaacaaac
    18961 taaattttca ctttggttaa atagtagatt tgatatacaa aatatagaag ataaaaatat
    19021 agctttcgat aaggtacgag ctacagtcaa tctaatggta gctagctctt tgatagcttt
    19081 aggtacatca ctcactctac ctctatctac cacgtatgta acatttatag tagctatggg
    19141 atcggcattt gcagataaag cttgggggcg agatacagct gtatatagag tatctggagt
    19201 agttactgtg ataggtggct ggtttttaac tgctttttta gcttttttat ttgcttcttt
    19261 actgggattt tttgcttatt ttacagattt tataggtgtt ttaattatca ttgctatatc
    19321 tactttagta tggataaggg ctaatcgtaa gcatagtcaa atagctagca agatcaaaaa
    19381 aactgatagt atctcgcata tagctagcaa aactgaagta aatatacaag taaataatta
    19441 tgaaaaatta gtatttcaat tattaaaaga aactacaaat actattatcg aatctctgaa
    19501 aaaagaaaaa atatccgtct taaaagatac actaaaaaat tatgaaaaac tcaaatcgga
    19561 agtatcagat tttaaaattc ttttgcctga tattatgtca caaataaaag ggctgagtac
    19621 agaagaatat caatattttt tagtaaaaat agatttattg gatcaaatat tagatattat
    19681 ttattatgta ttaaatccta cactgataca cttgcaaaat catcataaac cattaacaca
    19741 attccaaata gaccaaataa aatacatatt cgatataata gataatattt gcgatataat
    19801 aattgataaa aataaagaat tgagtaatga aataatacat ttattagaaa tggaaatagt
    19861 aaaagtgcga agaaatctaa tagctgtatc tctatctgat gataaaaata tcaaaatgcc
    19921 gcgtagagct actattttgg tttataatat attaaatgag tgcgacaacc tagttagact
    19981 attatatagt atggtagaaa ataattttaa gaaaccatct tttattttaa cttaaataaa
    20041 ttataaaatt taaaacagac taccgacaat gtactttttg taacttattg ccaatcaatt
    20101 ttattttcca aattttttga tatccttgtt gtaaaaaagc gaaatatatt tgtgagttaa
    20161 actgtacaag taaaataaaa aaaacaactt ttgcataggc acaatcatat aaaatgctat
    20221 ttactgcata ataaatttta gtatatctgt aagattaaat tgatttttct atgcattact
    20281 atctgtttcg attattttat aattattcat attactaatt ttatattgat aaattgttaa
    20341 caacaatata aacataaaaa ttgcaatata tgttttttta tctttaatta tatttgcata
    20401 tattttacct gatctaatgt acaaatattt aaaaaagata atttttttat tgacaaatat
    20461 ttttttatta acacaatttt tgacagcaca aaataatggt aatgctataa actgttcaaa
    20521 taaaacgatt agtttgctag gtggtgccta tattggtatt agtatttata catattatag
    20581 ttggtattat caatataata ctggcaaatt tcattttttt gatgatagca gagaatggtt
    20641 gggtatggat aaaataggtc attttttttc agctaatata ctaactaaat taaacaaaga
    20701 tctattaggg gattgtaggc agagtaaggt attggcacca ctgataactt ttggttatat
    20761 gacaagtata gaaataatgg atggatatag tgagggatgg ggattttcta taagtgatat
    20821 gctaacaaat attgctggca taatgacagc tattttacta gaagatcaag ttcaatggaa
    20881 attttcattt catagtaccg actgggcaaa taaaaggcct gagattttag gagaaaatat
    20941 cgggcaacag attctaaaag attataatgg tcaaacttat tggctttcat tgtcattaaa
    21001 taaaatatac gctcaattac ccaaattttt aatgctttct ttaggttatg gagctgatgc
    21061 tatgatatat ggtaatccta atgaacaaaa acaagtaaat ccttcattga taccttatcg
    21121 gcaattttat ttttcattag atatagattg gtggtattta atagataaag cgagtataag
    21181 aaaatggaaa tatatttttt atccattaaa atacatcaaa acacccatgc ctactattga
    21241 aatatcaaaa aataaagtaa aactacatcc tttttatttc taaaaaaatt aaaaaacata
    21301 gataaaaata ttttttaata tttaataaat aagtgcaaat aagtggcttt tcaaaaattc
    21361 caaaatctaa ttttagatat attgtaatta gatttatcaa tatatttaat ttatttatta
    21421 gttatattaa tttatattaa aagagagctc attaatgtat caaacaaaca attatcattt
    21481 ggctaatttt ttcatttctt ttattacttc taatggattg tgtgctttga atatagaagt
    21541 acctgctact aaaatatcaa ctcccgaggc tattagtttc tttgcatttt ctagattaat
    21601 accaccatcg atttctatta aacaatttaa tccttctttt tcaataaaat cgtgtaattt
    21661 ttgtacttta ttaatagaaa aaggtaaaaa tttttgtcca ccaaaaccag gatttactga
    21721 cattattaaa acataatcta tatatggcaa attatcatat attaattcta caggagtgaa
    21781 tggatttagt actaagcctg cttttacatt attatgttgc aatttttgta ctaataagtg
    21841 atgatgaata ttatcttcca cgtgaatact tatcatttct acacctattt cgaataaaaa
    21901 atcaaaatat tgttctggtt tttctaccat tagatgaacg tcaagtggtt tttgactgat
    21961 agtagctacc tgttctatta atggaaatcc tatagaaata tttggaacga aatgtccatc
    22021 cattacatct atgtgtagat aatctgcttc gctgatatta ataagttcta cttcttttcg
    22081 tagatctaaa aaattagctg ataataaaga tggagctact aatacttggt tgtgtatcat
    22141 atttattttt gttgttttat ttctttaaat tatttttaaa agttataagt aatgcctaat
    22201 aaaaaatgaa ccttgtaagt aggcaaatgc caataataat attgaggttt actaagtata
    22261 ttataaatat ttagataaaa agataaagaa ttattaaaga aatattcaat acctaaattc
    22321 caatctacaa aaggtgataa ctcgattgta tcattattat ctggctttaa ttgacatgca
    22381 tatcgcttcc ctacaaaagt taaattagta cctactaaaa attttttagc aaaaagatat
    22441 ttaacatcta aagtagacac aaaatttggt tcatgccaag gtttagcttc agatttagtc
    22501 tctaaatgat tatatctagt ttgccagttg atatttaatc tatcactata ccatttgata
    22561 ttcaaaaaag gtataagggc agtggtagta tcataaacaa cattaaaacg tgaatatggt
    22621 ctaatgatag tgtcattaat gaaaaatgca tattcattag ttttaccata agatacacct
    22681 atctctaatt gtaatttatt aaaaagtttg ctaactaaag aaccagtaaa atattgacgc
    22741 caaactaccg gtgtaggttt aataaattga tgtataaaag gattgatatg atataaagaa
    22801 tcaagagaat atacttctgt gaaactacca aagctagcat gtaattcaaa tatttctggt
    22861 acaaaaatta acttaccacc gatatatgga aaaacacgag cagaacccac actatctgct
    22921 ataacatcta ttgaaacccc cagtttcaac gcccatattt tttcttttaa ttgatatgat
    22981 agtaatgaat taaaaatgcc aaaattatat ttttctaaag tattattttc gaaataatga
    23041 ttatatttta aatacatagc gggtgtttgc gtaacatttt ttagtggcag ccatttaacg
    23101 ttttgataaa tattaactag tgcacctaat cgatattcat tgtttttgag ataggtaggt
    23161 aaaatagtcc ccgagatttt atattcctgt ttaaattcat taggattagt agaattgcta
    23221 acaacatcaa aattaatgtt tatcgtgttg tatttttgta aatgatcttt tttggttaaa
    23281 ctatcattag gatattctgt ttttttgaaa ccataataat gtattataga agcatcataa
    23341 cctgctgata aagtcaatgt aaatctattt tgtatccatt ctatatatcc attcagatta
    23401 tgtttccaat gcgctggagg tccatagttt gatattttac tatataaacc gaaaccatca
    23461 taataaattc cataatttct tttggtctta atagaattag cgatagataa atgccaatct
    23521 atcattttat tacctacacc aaatttagca taattgctag gcaaagcttc tataggttct
    23581 ttatctagct ttataggctt cagcggtgat ggattataag taataaaaat aggatgtggg
    23641 aatatatcat attgcaaaga atgtttttca gctttttctt catatatagt tggttggagt
    23701 ataggtttag caatatttgg taattcaagc ttatactcgc ctatcactat aactttttca
    23761 tcatattttt ttgttgtgtc agttttttgt gcaaaatttg ctattggaag gattaatatt
    23821 aaaatgaaaa tgtaaaaatt attttttttc atagtaaata taaaatattt tagcaaagtt
    23881 aaaaaaaaaa tctatggtga tatattttaa ataaaattca caaaaattaa tatattttaa
    23941 ataacttaga acaaacctag agtcacctaa ctcaagggaa agaatcgcta attattatag
    24001 atttacaagt aaaatgataa ggttgtgagt ttttataaaa ctaaaaaaaa ttatttaaaa
    24061 aatgtgtata atatttttct atacatttta atgataaata tcaataactg tttaaataaa
    24121 tcatatgaag atataaaaag ggtaaaaaaa ccacaacaaa tatgagtaaa ataaggaatg
    24181 ttttggaagg gagagtaata atagtcttca gtgtttaaat atacatagga cttgctctca
    24241 agtccctata tacataccac gctcgcctgt ctgatgatgc gagattgagc atcctaccta
    24301 catcgggcac cacattgttt aacaaatatt tatgcattac tacataccac ataatgtagc
    24361 tctgcaagta cttagtagcc acacctctga attgattatt tatccagcca acaaaagtat
    24421 ttacatgtcg tctaatggtt gctacactgt atactcctcg tttccttacc ctatctatca
    24481 ctatttcttt tcgctcaaga ttctgactct tcaactttct aaactcatca ttgtgtccga
    24541 aacatatcac actattagct gatactttgg tatttagcaa ttgctctatt tgctcgcgtt
    24601 ttattttacc taagcctaat tgcttaaaaa gcatattgcc agtcctatct ttagatgcta
    24661 taacggctac ttttttcttt ttcttttctc gtgcttctgc tagctcttct tcatttcgat
    24721 agcgtcttcc tttttcggag taattcatta acagttcctc tacttccacg attccgaaga
    24781 aattgacatt ggcttcgaaa tttctcaaag ctgctaaaat cctatgacgc caagcgaagc
    24841 ttgtaggtaa gctgatgccc acttctttag cacaagctct gagagttttg ccctcgagca
    24901 tacaa
//
LOCUS       DAOPRN010000028        23813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1755_length_23813_cov_103.438337, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000028 DAOPRN010000000
VERSION     DAOPRN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23813
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1755_length_23813_cov_103.438337"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1636
                     /locus_tag="PKI65_04670"
     CDS             377..1636
                     /locus_tag="PKI65_04670"
                     /inference="COORDINATES: protein motif:HMM:NF023021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB77767.1"
                     /translation="MKRLLLSLLFSFVTILFVYSQDTIKVMSYNLLNYGNYTSYCTTY
                     NNNVETKNEYLRTIIDYTLPDILGVVEISPNGTYIEDFKNNVLNQNGRDYYCNAPKTN
                     YSGSSIINMIYYDYRKVELKHWLALATDYRDINLYTFYFKNDALKNGDTVYLTCIVTH
                     LKAGHTDSDAIGRTAMAQKIMDFLSTINENTNYLIMGDFNVYSSSEGAYQQFTNHANQ
                     NIRFYDFINKYGVWSDNAYFAPYHTQSTHTTSDCFSGGGLDDRFDFILGNINTITGQK
                     GFKYVNDSYTTLGQDGQHFNKGLLDAPINTSAPMDVLEALYGNSDHLPVLAKFIIDHS
                     QSIIDITLPIAYYIQNNQLYINIFDAFSSDASIYIYDVHGRILFSDQISNQTDQYTLD
                     LNGLEKGIYLINIKTNDRFTSFKIVNI"
     gene            1697..2386
                     /locus_tag="PKI65_04675"
     CDS             1697..2386
                     /locus_tag="PKI65_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOB77768.1"
                     /translation="MKKSILSILFICFGIFQLSSQIDTTFIEPEFSIELKLSSKPQRF
                     FISFSQNFWLDLDKSTKDSMKLNDFQPSFGISFMYPISIGQSPFAFSFGLGMKVDNIR
                     FKSDLVSNDSMFYYNPELTFKKQKLVLPYIYIPVEFSYNSRGEHTFRLAIGGYFGLKM
                     RDHIKQISDDLRQKIYNIDNLEKYYYGLYMRVGYKSISININYNLSKVFVKDISNNMQ
                     TFSVGLCLSIF"
     gene            complement(2524..3765)
                     /gene="pepT"
                     /locus_tag="PKI65_04680"
     CDS             complement(2524..3765)
                     /gene="pepT"
                     /locus_tag="PKI65_04680"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOB77769.1"
                     /translation="MEMKEKLLDRFLRYVQIDTQSNEESDSCPSTEGQKVLANLLKEE
                     LIQIGVSHVEVDDNGYLYAYIPANKCDETPAIGFIAHLDTSPDMSGKNVKPQVHENYD
                     GKPIVLNEKLEIVLDPKDYPHLQYHIGKTIITSDGTTLLGADDKAGIAEIITAIEYLM
                     YNPDITHGPIYIAFTPDEEIGRGVDKFNLDKFPVKFAYTIDGSFEGELAYETFNAASA
                     KIFIQGNNIHPGDAKGKMLNAINLAVEFHSLLPSNQKPEYTTGYEGFYHLLQLNGTVE
                     NATLSYIIRDHNKEKFFQKKKFIENAIDFMNKKYPAQPFSLELKDQYYNMREVIEKNF
                     EVVELAKKAMESLNIEPKIIPIRGGTDGARLSFMGIPCPNIFAGGLNFHGKYEYVALE
                     TMEKATQVILKIVDLVKNFKN"
     gene            complement(3938..7474)
                     /gene="nifJ"
                     /locus_tag="PKI65_04685"
     CDS             complement(3938..7474)
                     /gene="nifJ"
                     /locus_tag="PKI65_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOB77770.1"
                     /translation="MKRDKKFITCDGNYAAAHISYIFSEVAAIYPITPSSPMAESVDE
                     WAAEGRKNIFGSTVKVIEMQSEAGAAGATHGSLQSGALTTTYTASQGLLLMIPNMYKI
                     AGELLPGVFHVSARTLAAHALSIFGDHSDVMATRMTGFALLATSSVQEIMDLAPVAHL
                     AAIKGRVPFLHFFDGFRTSHEIQKIEMLDAEDFIDILDWKAVEEFRSRSLNPNNSVIR
                     GTAQNPDIFFQAREAAQPFYDKIPDIVESYMQEIKKLTGREYHPFDYYGHSEAEYVIV
                     AMGSVADTVKDTIDYLMNLGEKVGLINVHLYRPFSEKYFLNVIPKSVKKIAVLDRTKE
                     PGATGEPLYLDVKNIFYDKPNAPEIYSGRYGLSSKDTTPSQIIAVYNNLKQKESKNHF
                     TIGIVDDISFSSLPLEKEVNISHQCIYSARFYGIGSDGTVGANKNSIKIIGDTTDKYC
                     QAYFAYDSKKSGGFTVSHLRFGDIPIRSPYLVTIPDFVACHVPAYLGKYDMLSGLKPD
                     GTFLLNTNWDVETTKEKLPNSIKKYLAVNNIKFYVINATQIAREIGLGNRTNSIMQSA
                     FFKITNIIPYDLAVKEMRKAVEESFGRKGEKVVQMNYQAIERGAEVVEVQVPEEWKNL
                     PDEDPICYPQDDAPEFISQVARVMNAQEGDKLPVSTFKGMEDGTFPTGTSKYEKRGIA
                     VTIPVWLPENCTQCNQCAYVCPHAVIRPFLLTDEELANAPAGTKSIKGSGATKEYNYT
                     LQVSVLDCTGCEVCINICPAKEKALVMEPLEDHLEEDERFKYMDQKVGYKDTVVDKYK
                     TVRGSQFAQPLFEFSGACAGCGETPYIKLITQLFGERMVIANATGCSSIYGASAPSMP
                     YTTSNRSGRGIAWANSLFEDNAEYAFGIASGIRKIREYIKENFVKFIANSNASAELKK
                     AMQEWIDGMNDAQSSEHTTEKILSLLSGAPSEIADFIRKYQQYLIKISVWGIGGDGWA
                     YDIGYGGLDHVLASGEDINILVLDTEVYSNTGGQSSKATPIGAIAKFAASGKKIRKKD
                     LGLMAISYGYVYVAQVAMGANINQYLKVIREAEEFPGPSLIIAYAPCIAHGIKGDMRQ
                     SQNQEDRAVKAGYWTLYRYDPRLANEGKIPFQLDSKEPDFTQFQEFLLSELRFSSLMR
                     SNPEAAKELFDEAERVAKWRYDRYVKLSQIIF"
     gene            complement(7772..9307)
                     /locus_tag="PKI65_04690"
     CDS             complement(7772..9307)
                     /locus_tag="PKI65_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77771.1"
                     /translation="MERIRLIFFLFFLFFLHPLFSQILSFDSSFYKTIEWFNYLENQG
                     FILSWNGTIYSENEQINISKNLLKNWNENKKNEVLKEILLPYWEAGYLLTQLNFNVDS
                     IVGNNIYISATLNTGNKIKLEAIKWNKPPPLKEKRLMKMLNIQLDEPITSYILFLQPK
                     TLLFDFIYLSDFPKLELVTDSTAILHLDINTKSKNNIEGMLGFAKLNSGIKNWQLNGF
                     LKGNFIDLIGWGEQWNIFINLGTGFQNINILFGVPYVAGLPLSIQGIFRMRVQDSTYG
                     YTNEEINFYHIDRSRRWKILFGGGQKQSTMFEKADSNLFSDYRYSYLNLGGQYSKLDN
                     NDNPNKGFKIDIHTNIGNSVIGAKKEINDKKNQLLAETFLNVISFIPIHRNVSLYFDC
                     LHRGVFSNKSILKNQTLLIGGINDILGIEEERITAKQLYKLESGVSLKGSQNTYIRFF
                     YQFAHVDHYQSEWGNYQSFGISFQLKTRIGWIWTAIAIPKEPNHNWDFASSRLHLKTS
                     VYF"
     gene            complement(9289..9963)
                     /gene="ung"
                     /locus_tag="PKI65_04695"
     CDS             complement(9289..9963)
                     /gene="ung"
                     /locus_tag="PKI65_04695"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOB77772.1"
                     /translation="MNPIIEKNWKEILWDEFQKPYFKEIKKFLIEEKKKYRVFPPGSL
                     MFNAFNLTPFNKVKVVIIGQDPYHGYGQAHGLCFSVPKGVTKPPSLINIFKELKEDLG
                     IDPPNHGDLSGWAKQGVFLINAILTVRENAPESHRNIGWEKFTNKAIQSLSEKKKHLV
                     FMLWGAYAQEKEKLIDPSKHLILKAAHPSPYSAKRGFFGCHHFSKANQYLIANDIEPI
                     RWSELD"
     gene            10084..10455
                     /gene="rpsF"
                     /locus_tag="PKI65_04700"
     CDS             10084..10455
                     /gene="rpsF"
                     /locus_tag="PKI65_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOB77773.1"
                     /translation="MVKHYETVFILTPVLSEEQAKETVKKYKEFLEQNGAQNIYEEFW
                     GLRKLAYPIQKKTTGFYHLFQYETERNDLVSLLELNFRRDERVMRYLTVKLDKYGIEW
                     NEKRKKQQAENKNKEKNKIEL"
     gene            10475..10744
                     /gene="rpsR"
                     /locus_tag="PKI65_04705"
     CDS             10475..10744
                     /gene="rpsR"
                     /locus_tag="PKI65_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOB77774.1"
                     /translation="MMAIDPGIKYLTPINIDTRVKKYCRFRKARIKYVDYKDPDFLIK
                     FVNEQGKILPRRVTGTSAKYQRKVAQAVKRARHLALLPFTTDLLK"
     gene            10763..11275
                     /gene="rplI"
                     /locus_tag="PKI65_04710"
     CDS             10763..11275
                     /gene="rplI"
                     /locus_tag="PKI65_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOB77775.1"
                     /translation="MALVEVILKEDVPKLGYKYDLVKVKAGYARNYLIPKGYALPATP
                     SNKKMYQEIKKQRSAKEEKMLTDAQALADKLNEVKLTIKTKVAQNTGRIYGSVTTNMI
                     ADAILEQYNFEIDRKTISIPETHIKQAGTYKAKAHIYRDIYAEFEFEIVSDEPIAENT
                     DVETSENEEK"
     gene            complement(11362..13629)
                     /locus_tag="PKI65_04715"
     CDS             complement(11362..13629)
                     /locus_tag="PKI65_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77776.1"
                     /translation="MSNFLRSLFFIFFVSSFVNDIKCQFFSNNITYDEYIFEINDKLT
                     INQNYNAGKLALTEFLIDLAHIHFYSIMESDSLTHYPETLYYLGEIYRYYEDYDMAKQ
                     YYQAYLMLPEHKDDFCYSHTMELLDNYENIYIYQKTDENITTRHLPSPLNTPYSEFAA
                     MEYGDAGDIFFSSYRPSITKQYSSLFTPFFKSDIYKTYYLPKGLAVPQKIILSKSSKQ
                     THNANLTFSPSQKFFSFTQCEYSSENCKIYWGEIDDSNNFINIKPVSINSDKSNQTHP
                     FLILDENGNGILFFTSNRPGGYGGYDLYFSIIKNFNFQVPINLGPVINTPGDEITPYY
                     DIRDSTLYFSSNFHIGLGGYDIFLSKGYLNSWQKVKNIGSPINSPFNDFYFYRNSYDF
                     DGYFSSNRKNSYFIQGQYCCYDIYTFYVEEKKLTITEPEITYTISLNDTINPLKEDII
                     SLLPITVYFHNDIPDPRSWDTTTKYSYDELYFAYLKLIDKYKKEYSKGLKGEDKLKAM
                     QEMEAFFNEEVIGGFDKLQRFTNLLLEDLKNGSSVTIKINGYCSPLHTSEYNINLSKR
                     RISSLKNYLLKINNGELAAFIEGTHPSGGKLRIIEEPMGKELASPYASDNPQDVQKSI
                     YSIAAAQERRAQISSYISDFKGSTLVYESGIYYTDSSFICISTSQLKNINSIDIPIYN
                     IGNAPFEIEILDLSKDCVQTMILDPKINPDKKTYLTVLLKEPCRSKPGLITIKLKTNI
                     QGDELHLISILIISD"
     gene            complement(13614..14630)
                     /locus_tag="PKI65_04720"
     CDS             complement(13614..14630)
                     /locus_tag="PKI65_04720"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOB77777.1"
                     /translation="MKKILILFCLIISISSLRCQDIHWSQILQNETFFNPAQIGLNSN
                     IIRLYTSAKLQWYSVTKPYQTYLLSTDGQILRRQPYMDKLSGNLIVFREFAGDASYGT
                     TSIALGLSYFKGLEGRYFHIISLGLQGWYNFRSFDFNKLYFGDQYNGDVFDPTLQTNE
                     QYIDEKLSYFTATIGIAYQLYFSNEQFLEINYALANIGNANISNSNGSRFILSLNHRS
                     SIRYYNEKLLKRNYLDAMLYYSYQKPFWELIPILKIMKPIEKNNSIKSIGGFIGDRIN
                     DAIIVGFAIDGRDWQGQISYDINTSSLNKASYGRGGFELSIYKYFNPPKIEKNKYKIL
                     CPIF"
     gene            complement(14627..18556)
                     /locus_tag="PKI65_04725"
     CDS             complement(14627..18556)
                     /locus_tag="PKI65_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB77778.1"
                     /translation="MNLKSIFALFIFFIFYCSTIKTQDFAEFNFIPNKGQWQNDFRYK
                     VKVDLNEIYIKDKSILFNILETGIHDSIYHRKMCPPNYNKSPLPFRVEAHAFEIFFDN
                     ANIPSFEEIDVLPFYHNYYLGNKAENWKTGIHPIRKVKLKELYDGIDVIISSKNGNLS
                     IDFFVLPMAKVKDIKLTVSGINKLKLDSTDIIITTSVGELRLNTPRAFQNGGEIPIRY
                     KINKNTISFDFPEGYDKKSELHIDPEIMFCSYSGATSDNWGYTATYDREGFLYAGGNS
                     FGMGYPTTLGAYDTIYGENVDAAISKYDTTGSYLIYSTYLGGSGAEVPISMITNDLNE
                     LYVLTITGSSDYPYTVGCFDSTFNGGTNYILTHVINYTNGSDLGITAFSADGTSLIAS
                     TYLGGTGNDGLNAAIALLHNYADDARGEIILGSNNEVYIAGSTYSTDFPTTLSSFQPN
                     PSLGQKGFVTCMDRYLSQITWSSYFDGSGDDAIYSIFLGRLDAIYIAGGTTSNDLPTT
                     TNAIMPNFQGNVDGFIARISNNGAFLTSCTYFGTPQYDQVFFVRTDKNDNVYLYGQTN
                     NTTNALVQNAGWYTNSGGQFITKLNRYLTSIYWSTRFGTTPGFINLSPSALTVDYCNN
                     IYLSGWGGSLNNGGTNGLPITSDAFQITTDNNDFYFLSIDGDANQLIFASYFGGHQSM
                     EHVDGGTSRFDRLGRLYQSICAGCGEHNDLPTTTGAWSNTNNSFNCNNGVVKIDFYLP
                     AIVADFNIPNLICLPDSVHFINNSHIPNISNTIFYWDFGDGSYSNLMNPIHYYSNAGV
                     YTITLIVSDVTSCNGSDTIQKIITILNNDIDTLNEVKICLGQSANIGITSLPAQGLTY
                     SWTPSDYLSNPNISDPIANPPYDITYTLSITGDVCAQIFVQPVSVINIIADAGNDTTV
                     CMSPITLYGSGSGDTDLSYIWSSNSNFTDTLNSSLNNNSVTVYPTNSHTYYLQVSNSL
                     CYDIDSVRVTLLPITIDANVQNPSCYDYCDGSISLEIYNANLPALFHWSNGMDTSYVD
                     SLCAGVYYVTVTDNVGCIKIDSIFIQAPPPLIAEIHSYPETCPDACNGSLTVNISGGT
                     PPYYYLWSNGATTQSNTNLCIGEYICTITDANDCEIINSDSVILDYILADAIAWVDHD
                     TIYQGQSTTIHSTYYPNVTYLWSPPSYLEDPTMPSTIATPPHTISYTIMIDDGYGCYF
                     YDTVDILVLEVFCDEPYIYIPNSFTPNDDNINDVLYIYGEYIESMYLAIYNRWGQKVF
                     ETNDKSKGWNGYYNGKPAPPGVYDYYLEIYCYNKQLFQKKGNITLLR"
     gene            complement(19002..19298)
                     /locus_tag="PKI65_04730"
     CDS             complement(19002..19298)
                     /locus_tag="PKI65_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77779.1"
                     /translation="MKNLVVVVLVASMMMLASCGNAKKKAEQAKLDSARIADSIAQVK
                     ADSARVADSLAQIEQARLDSIAQAEAQQQSKPQKPKAQQPQEQAQPVKAGQGKG"
     gene            complement(19396..22407)
                     /locus_tag="PKI65_04735"
     CDS             complement(19396..22407)
                     /locus_tag="PKI65_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB77780.1"
                     /translation="MKIKNLIKLYFTLIIIFSVNVIFSQQTYYFFTPQNTNDQVIDFF
                     NKKQFSILKQQTKIIPDKSYPTEAEYAYYKAYSAYMLHNDDAISLLLDYLDNYPLHVY
                     TNKAKFFIGNNYFWKKNYKKSIEYYNEVDIDNMVDSLVAELFFKRGYSYFTTEQYNEA
                     LVDLSNVMRYDTWYTPFAQYFIAYINYANGNYHVSLKEFKKIPQYTDDAALLNITDYH
                     ILFLEYLTNQVDSVIVHGEKMLPNALVTFLPDIHRMLAQAYWTKNELNLAKEHLVSYF
                     QLVPGKVTREDNYMLGYLYYSTGDYKSAIQYLTKVMTIKDSIYQSAAYHLGNAYLKTD
                     QKIFAQKAFYDAYSLDFDSLIREDALFNYAKLSYELDYDPYNLAINALQKYIEEYPQS
                     IRKNEAQELMLNLLMISNNYPRVIEIIEQMPRKNITIENMYQKACLNQALLDFKDRQY
                     NRSISYLNKAFNYGTDRTLKAKALFWKGEAYFALAKYDSALITFQTFVNYSPAKNLPE
                     YNKGFYNLGYSYLMTEKYSLAVDAFNNFISLADNEPTHIKYDAYLRYADALFLTKKID
                     EAAKMYKKVIDEYPKQADYAYLSYAKILGIKGQLKEKINYLDLLIINFPNSPYIPEAL
                     YEKALSQELIDETNAIKTYHEVYTKYPQHPLANKAMLKEALILRNQKEYDKAIQVLEE
                     LIIKYKNSESERDAWIALRETYTDADRINEFFAFAQKYGKSVSADEQEELIYTSAENK
                     FMSNDYQSAITAFDQYLTKFPDGAYSIHAHFYRGEANYALGNFENALQDYLYVINAQN
                     SRFTESSLLKAARIYYNRNEYEACIPLYEQLLTIAQVKMNRIEAMRNLLKAYELTNNY
                     NKAIEHANNILESLDMDDNDKLLANLTKARIYLQMQQISECVDICNALSKQKSEIGAE
                     ATFYLASIEYMNGNLNNAEQKIFYLVENFAVYEDWIAKGFILLSDVYLSRGDLYQAKA
                     TLEHLINNYKGDQSIIDIAIEKYNDLIQIQE"
     gene            complement(22610..23638)
                     /locus_tag="PKI65_04740"
     CDS             complement(22610..23638)
                     /locus_tag="PKI65_04740"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOB77781.1"
                     /translation="MLDNNEILELNDEFYEHYKIIVDPGQSLMRLDIFLTQKIENISR
                     TKIQNAAMSGCILVNDVPKKSNYKVKPGDIIRVMLPHPPIPIEVLPEKIDLSLLYEDE
                     DIIIINKPAGMVVHPGYNNYTGTMLNGLLYYFQNILKTDQFPLLLHRIDKNTSGLLVV
                     AKNEYAQQIISKQFFEHSVKRIYITVVWGNVENDTGEIIGYLSRDKKDRRLMSLSKNA
                     NIGKYSHTNYRVIERYGFATLIECKLYTGRTHQIRAHMKAIGHPIFNDEDYGGNKIVY
                     SGYGSNYKQFILNSFKICSRQALHAKTLGFIHPMTNQEIVFDSELPDDITKLINLFNN
                     YKSTYNLG"
BASE COUNT         8263 a         3642 c         3319 g         8589 t
ORIGIN      
        1 aggtatttgc tggtgttttc tacaattttt ttcatagact aaaatttttg caaaaatata
       61 aaaattattt aatatataaa cttgaaaaac ttaaacattt attaattttt ttattataga
      121 acagttaaat atcaaccata aagttctctg agaatgcaca aagaacacag aggagaatgt
      181 aattaaaata gctacaaata gctctgtgtg ctaaaaaatt aacctaaata atttacccac
      241 aataaacgtt atagaaccat tttttttatt aacttttata catatattca tactttcttt
      301 ttaaatataa acctaaaaac ttatttcata attaaatcga aatttttata taaatttgtt
      361 aaaaaaaaat taaattatga aaaggttatt attatcactt ttattcagct ttgtaacaat
      421 tctttttgtc tattctcaag atactatcaa agtgatgtct tacaatttgt taaattatgg
      481 caattatacc tcatattgta ctacttataa taataatgta gaaactaaaa atgaatattt
      541 gagaacaatt atagactaca ccctcccgga tattttaggt gttgttgaga tttctccaaa
      601 tggcacttat atagaagatt ttaaaaacaa tgttttaaat cagaatggca gagattatta
      661 ttgtaatgca cctaaaacaa attactctgg cagttctatt attaatatga tatattacga
      721 ctataggaaa gttgaactaa agcattggtt agcattagct acggattatc gtgatatcaa
      781 tttatatact ttttatttta aaaatgatgc tctaaaaaat ggtgatactg tataccttac
      841 ttgtatagta acacatctaa aagctggaca tactgatagt gatgctatag gtagaacagc
      901 aatggctcaa aaaatcatgg atttcttgag tactataaat gaaaatacta attatctaat
      961 aatgggtgat tttaatgttt actcatcatc agaaggggct tatcagcaat ttactaatca
     1021 tgctaatcaa aatattcgtt tttatgattt tataaataaa tatggagtat ggtctgataa
     1081 tgcatatttt gcaccttatc atactcaatc cactcataca acttctgatt gtttcagtgg
     1141 aggaggttta gatgatagat ttgattttat tttaggaaat atcaatacta ttactgggca
     1201 aaaaggtttt aagtatgtaa atgattcata tacaactcta gggcaagatg gccagcattt
     1261 taataagggg ttgttagatg ctcctattaa tactagtgct ccgatggatg tattagaggc
     1321 tttatatggc aatagtgatc atttaccagt tttagctaaa tttattatag accatagtca
     1381 gagtattata gatattacac ttcctatagc ttattatatt caaaacaatc aattatatat
     1441 aaatatcttt gatgcgttta gttccgatgc ttctatatat atatatgatg tacatggcag
     1501 aatattattt tctgatcaaa tatctaatca aacagatcaa tatactttag atctgaatgg
     1561 tttagaaaaa ggtatttatc tgataaatat taaaactaat gatagattta catcttttaa
     1621 aattgtgaat atttaatttt ttaaatttat taatttatta ttaaaattta tatattatat
     1681 ttgtaaaaaa ataattatga aaaagtcgat tttatcaata ttattcattt gttttggtat
     1741 atttcagctc tcatcacaaa tagatactac atttatagaa ccagaattct caatagaact
     1801 aaaattgagt tccaaaccac aaagattttt tatttcattt tctcaaaatt tttggcttga
     1861 tttagataaa tctacaaagg atagtatgaa attaaacgat tttcagccat catttgggat
     1921 cagtttcatg tatcccatta gcataggaca aagtccattt gcattttcgt ttggtctagg
     1981 tatgaaagta gataatatta gatttaagag cgatctagta tctaatgatt ctatgtttta
     2041 ttataatcca gaattgactt ttaaaaaaca aaaattggtc ttaccttata tctatatacc
     2101 tgttgaattt tcgtataatt ccagagggga acatacattt cgtttggcta taggtggtta
     2161 ttttggattg aaaatgcgtg atcatataaa acaaatatcg gatgatttaa ggcaaaaaat
     2221 ttataatatt gataatttag aaaaatatta ttatggttta tatatgcgag taggatacaa
     2281 atccataagt ataaatatta attacaatct atctaaagta tttgttaaag atatttcaaa
     2341 taatatgcaa acattttcag tagggttatg tttatctata ttttagaact ttgctttttt
     2401 aaaagttcta caattaataa ttaaaaaaaa attcaaaaat atattataaa taagttatta
     2461 agatttagta ttatttgaat gtggtaagat atttaaatct agcaaagatc aaaattaaat
     2521 cacttaattt ttaaaatttt taactaaatc aacaattttt aaaattacct gtgtagcttt
     2581 ttccatagtt tctaatgcca cgtattcata tttaccatga aaatttaaac ctccagcaaa
     2641 aatattagga caagggatac ccataaaaga tagcctagca ccatcagtac cgcctctgat
     2701 aggaataatt ttaggttcta tattcagaga ttccatagct tttttagcta gctctactac
     2761 ttcaaaattt ttctctatca cttctcgcat attatagtat tgatctttta gttctaaact
     2821 aaatggttgt gcaggatatt ttttattcat aaagtcaata gcgttttcta taaatttctt
     2881 tttttggaaa aatttttctt tattatgatc tctaattatg taagataatg tggcattttc
     2941 tactgtacca tttaattgta acaaatgata aaaaccttca tatcctgtag tgtattctgg
     3001 tttttggttt gaaggtaata aagaatgaaa ttctacagcc aagtttatag cgtttagcat
     3061 tttgccttta gcatcacctg gatggatatt gttaccttgt ataaaaattt tagcagatgc
     3121 agcattgaaa gtttcatatg ctagttctcc ttcgaagcta ccatcaatag tatatgcaaa
     3181 tttcactggg aatttatcta aattaaattt atccacccct ctacctatct cttcatcagg
     3241 ggtgaaagca atataaatag gaccatgagt aatatcagga ttgtacatta aatactcaat
     3301 tgctgttatt atctcagcga taccagcttt gtcatcagca cctaatagag ttgtaccatc
     3361 agaagtgatt atagttttac caatatgata ctgtagatgt ggataatctt taggatctaa
     3421 tactatttcg agtttttcat ttagtacaat gggcttgcca tcataatttt catgaacttg
     3481 tggttttaca ttttttccac tcatatcggg tgatgtatct aagtgagcta taaatcctat
     3541 agctggtgtt tcatcacatt tattagcagg tatgtatgca taaagatatc cattgtcatc
     3601 tacctctaca tgtgaaactc ctatttgtat caattcttct tttaataaat ttgctaaaac
     3661 tttttgtcct tcagtggaag ggcaactatc tgattcttca ttgctttgtg tatctatctg
     3721 cacataacgt aaaaaacgat ctaacaattt ttctttcatt tccataacta tttttttaca
     3781 aagtaaagta aaaaaaggga tactatacta caaaaatcat aagaatcaag aaacttattt
     3841 ttaattaatg taatagtaat caatataaaa aggggagata cactaatttg tgaatcaccc
     3901 ctttttttta aaaatacaat atatgtatgc taaattatta gaatattatt tgagataatt
     3961 tgacatatct atcatagcgc catttagcta ctctttctgc ttcatcgaaa agttctttag
     4021 cagcttcggg attagatctc ataagagaag agaatctaag ttcagataaa aggaactcct
     4081 ggaattgagt aaaatctggt tctttgctat ctaattgaaa aggaattttt ccttcattag
     4141 ctaagcgtgg atcataacga taaagtgtcc aataaccagc ttttactgct ctatcttctt
     4201 gattttgaga ttgtctcata tcacctttta tgccatgagc tatacatgga gcataagcaa
     4261 tgataagaga tgggccagga aattcttctg cttctctgat gacttttaga tattgattga
     4321 tattggctcc catagctacc tgggctacat aaacataacc ataagaaata gccattaatc
     4381 caagatcttt ttttctaatt tttttgcctg aagcagcaaa tttagctata gcacctatag
     4441 gggtagcttt agatgattga ccgcctgtat tagaataaac ttcggtatct aaaaccaaaa
     4501 tgtttatatc ttctccgcta gctaatacat gatctaaacc tccataacct atatcataag
     4561 cccagccatc accacctata ccccagacac taatttttat taaatattgt tgatattttc
     4621 taataaaatc tgcaatttca ctaggggcac ctgataataa tgataatatt ttttcagttg
     4681 tatgttcaga actttgggca tcattcatgc catctatcca ttcttgcata gcttttttta
     4741 gttcagcaga agcattacta ttagcgataa attttacaaa attttcttta atatattctc
     4801 tgatcttacg aataccacta gctataccaa aagcatattc agcattatct tcgaataagc
     4861 tatttgccca agctatacct ctaccactac gattagatgt agtatatggc atagaaggag
     4921 cactagctcc atatattgac gagcaaccag tagcatttgc tattaccata cgctcaccaa
     4981 aaagttgagt tattagttta atataaggag tttcaccaca accagcacat gcaccgctaa
     5041 attcaaataa tggttgagca aattggctac ctctgacagt tttatattta tctacgacag
     5101 tatctttata tcctactttt tgatccatat atttaaatct ttcgtcttct tcgaggtggt
     5161 cttctaatgg ttccattacc aaggcttttt ctttagctgg gcagatattt atacatactt
     5221 cgcaacctgt acaatctaat acactcactt gtaatgtata gttatattct ttagtagcac
     5281 cactaccttt aatagattta gtgcctgcag gggcattagc taattcctca tcagtcaata
     5341 aaaaaggacg aataacagca tgtggacaaa cataagcgca ttgattacat tgagtacaat
     5401 tttccggcaa ccaaactggt atagtgactg ctatgccacg tttttcatat tttgatgtgc
     5461 ctgtggggaa tgtaccgtct tccattcctt taaatgtgga tacaggcaat ttatcccctt
     5521 cttgagcatt cattacgcgt gctacttgag aaatgaattc tggagcatcg tcttgtggat
     5581 agcaaatagg atcttcgtca ggtaaatttt tccattcttc aggtacttgt acttctacta
     5641 cttcagcacc tcgttcgata gcttggtaat tcatttgcac tactttttca ccttttctac
     5701 caaaagattc ttctacagct tttctcattt ctttgacagc tagatcataa ggaataatat
     5761 ttgtgatttt aaagaaagct gattgcatta tagaattagt acgattacct aaacctattt
     5821 ctcgtgctat ttgagtagca tttattacat aaaatttaat attatttact gctagatatt
     5881 ttttaataga attaggtagt ttttcttttg tagtttctac atcccaattt gtatttaata
     5941 ggaaagtacc gtctggtttc aatccactaa gcatatcata tttgcctaaa tacgcaggca
     6001 catgacaagc aacaaaatct ggtatagtaa ctaaataggg actacgtata ggaatgtcac
     6061 caaatcgcaa atgtgataca gtaaacccac cggatttttt agaatcataa gcaaaatatg
     6121 cttggcaata tttatctgta gtatcgccaa tgatctttat tgaattctta ttagcaccta
     6181 cagtaccatc agaaccaatg ccataaaatc tagcagaata aatacattga tgtgaaatat
     6241 ttacttcttt ttccaatggc aaagatgaaa aagaaatatc gtctacaata ccaatagtga
     6301 agtgattttt agattctttc tgctttaaat tattataaac agcaattatt tgtgatggag
     6361 tagtatcttt agagctgaga ccgtatcgac cactataaat ttctggtgca tttggcttgt
     6421 cataaaagat attttttaca tctaagtaaa gtggttcacc ggtagcacca ggttctttag
     6481 tcctatctaa tactgcaatt tttttaacag atttgggtat tacattgagg aaatattttt
     6541 cactaaaagg acggtagaga tgtacattta taagaccgac tttttctcct agattcatta
     6601 aataatctat tgtatctttt actgtatcag ctacagatcc catagctacg ataacatatt
     6661 cagcttctga gtgtccataa taatcaaaag gatggtattc tctaccagtc aattttttaa
     6721 tttcttgcat atatgattct acaatatcag gtattttatc atagaatggc tgagccgctt
     6781 cacgagcttg gaaaaagata tcagggtttt gtgcagtgcc acgtataact gaattgttgg
     6841 gattgaggga tctactacga aattcttcta cagctttcca atctaaaata tcaataaaat
     6901 cttctgcatc gagcatttct attttttgta tttcgtgcga agttctaaag ccatcaaaga
     6961 aatgtaagaa aggtactcta cccttgatag cagctaaatg cgctactggt gctaagtcca
     7021 tgatttcttg tacgctgctt gtagcaagca atgcaaaacc tgtcatccta gtagccatca
     7081 catctgagtg atcgccaaaa atagataatg cgtgagcagc tagagtacga gcagacacat
     7141 gaaatactcc aggtaaaagc tctccagcta ttttatacat gttaggtatc atcaatagta
     7201 atccttgact cgctgtatat gtagtagtga gtgcaccact ctgtaagctt ccatgcgtag
     7261 cacctgctgc accagcttcg ctttgcattt caataacttt aacagtagaa ccaaaaatat
     7321 tttttctgcc ttcagcagcc cattcatcta cactctctgc cattggtgat gatggggtaa
     7381 taggataaat ggcagctacc tcactgaaaa tataacttat gtgtgctgca gcatagttac
     7441 catcacaggt aataaatttt ttatctctct tcatttttat tttatttttg cgttaatttg
     7501 tttgcgaaat taatcaaaat tattataatt gtcaaaccat tttggtattt atttttaaat
     7561 aaattaatga gatataatat gtttttaacc ttaattaagc agataatata aaatataata
     7621 gttgttttgc tatttctatg aatatcactt gcaattaata aaagtattac ttgtagagcg
     7681 taacttaaac aattatatca aaaattttat aaaactataa tgattttttt taatagagtt
     7741 gtgtattaaa atgatcaaac cagataattt tttaaaaata taccgaagtt tttaaatgaa
     7801 gtctactact agcaaagtcc caattgtgat taggttcttt aggtatagct attgctgtcc
     7861 atatccagcc gattcgtgtc tttaattgaa atgaaatgcc aaaactttga taatttcccc
     7921 attcagattg ataatgatct acgtgagcaa attgataaaa aaatctaata tatgtgtttt
     7981 ggctcccttt caatgaaact cctgattcta acttatataa ttgttttgca gtgattcttt
     8041 cctcttctat acctaatata tcatttatgc ctcctattaa tagagtttga ttttttaata
     8101 tcgatttgtt tgaaaaaaca cctctatgta aacaatcaaa atacaatgat acatttctat
     8161 gaattggaat aaaagaaata acattcagaa aggtctctgc taataattga ttttttttat
     8221 catttatttc tttttttgct ccaattactg aattacctat gttagtatgt atatctattt
     8281 taaatccttt attaggatta tcattattat ccaatttaga atattgtcca cctagattaa
     8341 ggtagctgta tctataatca ctaaataaat tactatcggc tttttcaaac atagtagatt
     8401 gcttttgtcc accaccaaac aatattttcc atcgtctcga tctatctatg tgataaaagt
     8461 taatctcttc atttgtatat ccgtaagtac tatcctgtac tctcattctg aaaatccctt
     8521 gaatagataa aggtaatcca gcgacataag gaacaccaaa caaaatattt atattttgaa
     8581 aaccagtgcc caaatttata aatatattcc attgttcgcc ccatcctatc agatctataa
     8641 aattaccttt taaaaaacca ttaagttgcc aattttttat tccactattt agcttagcga
     8701 agcctagcat accttcaata ttatttttag attttgtatt tatatctaag tgcaatatag
     8761 ctgtactatc agtcactaat tctaatttag gaaaatcaga taaataaata aaatcaaata
     8821 ataatgtctt aggttgtaga aataatatat aactagtaat gggttcatcg agttggatgt
     8881 ttagcatttt cataagtctt ttttctttta atggaggagg tttattccat tttattgcct
     8941 ctaattttat tttgttaccc gtattaagag tagctgaaat ataaatatta ttgcctacta
     9001 tgctatcaac attaaaattt aattgagtta ataaataccc agcttcccaa taaggtaaaa
     9061 gtatttcttt taaaacttca ttttttttat tttcattcca attttttaat aaatttttag
     9121 aaatgtttat ttgttcattt tcactgtaaa tagtgccatt ccacgataaa ataaatcctt
     9181 gattttctaa ataattaaac cattctatag ttttataaaa tgatgaatca aaagataaaa
     9241 tttgagaaaa taaaggatgc aaaaaaaata aaaaaaatag gaaaaatatt aatctaattc
     9301 gctccatctg ataggttcta tgtcattagc aatcaagtat tgattagctt tagagaaatg
     9361 atgacaaccg aagaaacctc ttttagcact gtaaggcgaa ggatgcgcag cttttaagat
     9421 aaggtgtttg ctaggatcta ttaatttttc tttttcttga gcataggcgc cccataacat
     9481 aaagactaaa tgcttttttt tctcagataa tgattggata gctttgttag tgaatttttc
     9541 ccatcctatg ttacggtgag attcaggtgc attttctctg actgttaata tagcattaat
     9601 caaaaagacc ccttgctttg cccatccgct caaatctcca tgattaggtg gatcgatgcc
     9661 tagatcttct tttaattctt taaatatatt gatcaagctg ggcggtttag taacgccctt
     9721 tggcactgaa aagcataatc catgagcttg tccataacca tgatatggat cctgacctat
     9781 gataactacc ttaactttat taaaaggcgt taaattaaat gcattaaaca tcaatgatcc
     9841 aggtggaaaa actctatatt ttttcttttc ttctattaaa aattttttta tttctttaaa
     9901 ataaggcttt tgaaactcat cccataatat ttctttccag tttttttcta taattggatt
     9961 tatttccata atcaatgatt tatgcaaata taataaaaaa aagtaagtga tttaaaattt
    10021 tgatttttta gtttaaaaaa aaatttgtaa ctttgcaaac taaaaaatta aaaaaaaatt
    10081 tttatggtaa aacattatga aaccgttttc attttaactc ccgttttgtc tgaggaacag
    10141 gcaaaggaaa cggtaaaaaa gtacaaagaa tttttggagc agaatggtgc tcaaaatatt
    10201 tatgaagaat tctggggact gcgaaagtta gcgtatccca ttcaaaaaaa gactactggt
    10261 ttttatcatt tgtttcaata tgaaactgaa agaaatgatc tagtatcatt attagagctt
    10321 aattttagac gcgatgagag agtgatgcgt tatctcacag tgaaactaga taagtatggc
    10381 atagaatgga acgaaaagag aaaaaaacaa caagccgaaa ataaaaataa agaaaaaaat
    10441 aaaatagaat tataattatt aaaaaaaaat taaaattatg gcaatagatc caggaattaa
    10501 atatttgact ccaataaata tagatacaag agtaaaaaaa tattgtcgct ttcgcaaagc
    10561 gaggattaaa tatgtagatt ataaagatcc agatttttta atcaaatttg taaatgagca
    10621 aggtaaaatt ttaccacgta gagtcactgg tacctcagct aaatatcaaa gaaaagtagc
    10681 tcaagctgtc aagagagcca ggcacctagc tcttttacca tttactactg atttattaaa
    10741 ataaaaaaat taaaaaaaaa tcatggcatt agtagaagta attttaaaag aagatgttcc
    10801 taaattggga tataaatatg atttagtaaa agtaaaagct ggctatgctc gtaactattt
    10861 gatacctaaa ggatatgctt tgccagctac tccatcaaat aaaaaaatgt atcaagaaat
    10921 taaaaaacaa agaagtgcaa aagaagaaaa aatgctaaca gatgcacaag ctcttgctga
    10981 taaactaaat gaagtcaaat taacaataaa aactaaagta gctcagaata caggcagaat
    11041 atatggcagt gttactacaa acatgatagc tgatgctatt ttagaacaat ataatttcga
    11101 aattgatcgc aaaactattt ctatcccaga aacacatatt aaacaagcag gcacttacaa
    11161 agcaaaagct catatttatc gtgatattta tgcagagttt gaatttgaaa tagtaagcga
    11221 tgagccaata gctgaaaata ctgatgtaga aacatcagaa aacgaagaga aataagaaat
    11281 atttttaaca tattttttta tattgtaatt aaaaatttta ggcattagga gtttttagtt
    11341 tattaaaata aaattttctt attaatcact tattattaat atgcttatca agtgtaattc
    11401 gtcaccttga atattagttt ttaatttaat agttattagt cctggctttg atcggcatgg
    11461 ttcttttaat aagactgtaa gataggtttt tttatctgga tttattttag gatccaaaat
    11521 cattgtctgc acacaatctt tagataaatc taaaatttct atttcaaaag gtgcatttcc
    11581 aatattataa ataggaatat cgatgctatt aatatttttt agctgactag tactaataca
    11641 gataaaacta gaatctgtat aataaattcc cgactcgtat acaagggtag aacctttaaa
    11701 atctgagatg taagaagata tttgagctct tcgttcttga gctgctgcta tgctataaat
    11761 agatttttgt acatcctgtg gattgtcaga tgcataaggc gaagccagct ccttgcccat
    11821 cggctcttct attatcctta atttgccacc tgagggatga gtaccttcta tgaatgctgc
    11881 taattctcca ttatttattt ttaatagata attttttaaa ctagaaattc ttctctttga
    11941 tagattaata ttatattcag aggtgtgtaa tgggctacaa tatccattga tttttatcgt
    12001 aacactactt ccatttttta aatcttctaa aagtaaatta gtaaaacgtt gtagtttgtc
    12061 aaaaccacct ataacctctt cattaaaaaa tgcttccatt tcttgcatcg cttttagctt
    12121 gtcttcacct ttcaatcctt tactatattc ttttttgtat ttatcaatta atttcaaata
    12181 tgcgaaatac agttcatcat aactatattt ggtagtagta tcccagcttc ttggatcagg
    12241 aatgtcatta tgaaaataaa ctgtaatcgg caataaagat attatatctt cttttaatgg
    12301 attgatagta tcatttagtg aaatagtata tgtgatttca ggttctgtga tagttaattt
    12361 tttttcttct acataaaaag tataaatatc ataacagcaa tattgccctt gaataaaata
    12421 agaattcttg cgattagatg aaaaatatcc atcaaagtca tagctatttc tataaaaata
    12481 aaaatcatta aatggtgaat tgatgggcga tcctatattt tttacttttt gccaactatt
    12541 tagataacct ttagataaaa atatatcata tccacctaag ccaatatgga aattactact
    12601 aaaatataaa gtagaatccc ttatatcata atatggcgtg atctcatcac caggggtatt
    12661 gattacaggg ccaagattga taggaacttg gaaattaaaa tttttaatta tggaaaaata
    12721 taaatcatag ccgccatatc caccaggtct attagaggta aaaaataata ttccgttacc
    12781 attttcatct aatatcaaaa atgggtgggt ttgattactt ttatcactat ttattgaaac
    12841 tggtttaata ttaataaaat tattactatc atctatttcg ccccaataaa ttttgcagtt
    12901 ttcgctggaa tattcacatt gagtaaaact gaaaaatttt tgacttgggc taaaagttag
    12961 attagcatta tgtgtttgtt tactcgattt agaaagtatt attttttgtg gtacagctag
    13021 cccttttggc aaataataag ttttataaat atcggatttg aaaaatgggg tgaatagtga
    13081 actatattgc ttagtaatac ttggcctgta agaagaaaaa aatatatctc ctgcatcgcc
    13141 gtattccatt gctgcaaatt cactataagg tgtattgaga ggtgatggca aatgtctcgt
    13201 agtaatattt tcatcagttt tctgatatat ataaatattt tcataattat ctaataactc
    13261 catagtatgt gaatagcaaa aatcatcctt gtgctctggc aacatcagat atgcttgata
    13321 atattgctta gccatatcat agtcttcata atatctgtag atttctccta aataataaag
    13381 agtttctgga taatgagtta aagaatcaga ttccattata gagtagaaat gtatatgagc
    13441 tagatcaatt aaaaattcag ttaacgctaa tttgccagca ttataatttt gatttatggt
    13501 taatttatcg ttgatttcaa aaatatattc atcataagta atattattag aaaaaaattg
    13561 acattttata tcattaacaa aggaactaac aaaaaatata aaaaataatg atcttaaaaa
    13621 attggacata atattttata tttatttttc tcaatttttg gtggattaaa atatttataa
    13681 atactaagtt cgaagccacc acgaccataa ctagctttat taagtgatga agtatttatg
    13741 tcataagaaa tttgtccttg ccagtccctg ccatcaatag caaaaccaac aattattgcg
    13801 tcattgatcc tatcacctat aaatccgcca atgcttttga tagaattatt tttttctata
    13861 ggtttcatga tttttagtat tggtattagc tcccaaaatg gtttctgata tgaatagtag
    13921 agcatagcat ctaaataatt tctttttaat aacttttcat tgtaatacct aatagaagag
    13981 cgatgattta gcgataaaat aaatctactt ccattactat tagatatatt tgcattacct
    14041 atattagcta gagcataatt aatttccaga aattgttcat tagaaaaata aagctgatat
    14101 gctataccta tggtagcagt gaaatatgat aatttttcat caatgtattg ttcattcgtt
    14161 tgtaaggtag gatcaaacac gtcgccatta tactgatcac caaaatataa tttattaaaa
    14221 tcaaaggatc taaaattgta ccaaccttgt aaaccaagac taataatatg aaaatatcta
    14281 ccctctaaac ctttaaaata agacaatccc agagcaatag aggtggttcc atagctagca
    14341 tcgccagcaa attctctaaa aactattaaa ttaccactta gcttatccat gtatggttgt
    14401 cttcttaata tttgcccatc agtggatagt aaataggttt gatatggttt ggtaacagaa
    14461 taccattgta gtttagccga ggtatataaa cgaataatat tggaattcaa tcctatctga
    14521 gcgggattga aaaaagtttc attttgtaat atctgactcc aatgtatatc ctgacatcgc
    14581 agagatgata tagatataat taagcaaaat aaaattaaaa tttttttcat cttaatagtg
    14641 taatgttacc ctttttttga aaaagttgtt tattataaca ataaatttcc agataataat
    14701 catatacacc tggtggagcg ggtttgccat tataatagcc attccaacct tttgatttat
    14761 cattagtttc aaaaactttt tgaccccagc gattataaat agctaaatac atagactcaa
    14821 tgtattctcc ataaatatat aaaacatcat ttatattatc gtcattggga gtgaaactat
    14881 tagggatgta aatatatggt tcatcgcaaa acacttctaa aacaaggata tctactgtat
    14941 cataaaaata acaaccatag ccatcatcga tcattatagt atatgaaata gtatgcggcg
    15001 gagttgctat tgtagatggc atagtaggat cttctaaata tgatggtgga gaccacaaat
    15061 aagtaacgtt aggataataa gtagaatgaa tagtagtaga ttgtccttga tagatagtgt
    15121 catgatctac ccaggctata gcatcagcaa gaatgtagtc caaaatcacc gaatcactat
    15181 taataatttc acaatcattt gcatctgtta tagtgcaaat atattcacct atacatagat
    15241 tagtgttaga ttgagtagta gctccattag accataaata ataataaggc ggagtgcccc
    15301 cagaaatatt gacagtaagt gaaccattac atgcatctgg acaagtttct gggtagctgt
    15361 gaatctctgc tattaatggt ggtggagcct gaataaaaat tgaatctatc ttaatacaac
    15421 caacattatc ggtaaccgtt acataatata cacctgcgca taatgaatct acataagaag
    15481 tatccatccc attactccaa tgaaataaag caggtaaatt agcattatat atttctaatg
    15541 aaatgctacc atcgcaataa tcgtaacaac ttggattttg aacattagca tcgattgtaa
    15601 taggcaacaa agttacccta acgctatcta tatcatagca taagctatta ctcacttgca
    15661 gataataagt atgactatta gtaggatata ctgtaacaga attattatta agtgaactat
    15721 ttaatgtatc agtaaaatta gaatttgatg accaaatgta ggataaatct gtatcaccgc
    15781 taccactacc atataaagtg ataggtgaca tacatacagt agtatcattg cctgcatctg
    15841 ctataatgtt tataacactt accggttgaa caaaaatttg ggcgcagaca tcgccagtga
    15901 ttgataaagt ataagtaata tcataaggag gattagcaat gggatcgctg atattaggat
    15961 tagacagata atctgatggt gtccatgagt aagtaagccc ctgtgctggt aaagaagtga
    16021 tgcctatatt agcagactgt cctaaacata tttttacttc atttaatgta tctatatcat
    16081 tatttaatat agttataatc ttttgaatag tatcagagcc attgcagcta gtaacatctg
    16141 aaactattaa agtgatagta tatacacctg cattagaata gtaatgaatt ggattcatta
    16201 aattagaata actaccatca ccaaaatccc aataaaaaat agtatttgat atgttaggga
    16261 tatgactatt attaataaaa tgtacgctat caggtaaaca aattaaattt ggaatattga
    16321 aatcagctac aattgctggt agataaaaat caatttttac tacaccatta ttacaattaa
    16381 aagaattatt agtattagac catgcaccag tagtagtagg caaatcatta tgctcaccac
    16441 atcctgcaca aatagactga tataaacgac ctaatctatc gaaacgacta gtcccaccat
    16501 ctacatgctc catactttgg tgaccaccaa aatatgaagc aaaaataagc tgattagcat
    16561 caccatcaat agagaggaaa tagaaatcat tattatcagt agtaatctga aaagcatcag
    16621 aagtaatagg taacccatta gtaccaccat tatttaatga tcccccccat ccagacaaat
    16681 aaatattatt acaataatcc actgttaatg ctgatgggct caaatttata aatccaggtg
    16741 tggtgccaaa tctagtagac cagtagatag aagtcaaata tctatttaat tttgtaataa
    16801 attgaccacc agaattagta taccatcccg cattttgtac taaagcattt gtagtattat
    16861 tagtttgccc atataaataa acattatcat ttttgtctgt ccttacgaaa aacacttgat
    16921 catattgagg ggtaccaaaa tatgtacaac tagttaaaaa tgcgccatta ttgctaattc
    16981 tagctatgaa tccatctaca tttccttgaa aatttggcat aattgcatta gttgtagtag
    17041 gtaaatcgtt acttgtagta ccaccagcaa tgtaaatagc atctagtcta ccaagaaaaa
    17101 tgctataaat agcatcatca ccactaccat caaaataact actccacgtt atttgtgata
    17161 aatatctatc catacatgta acaaaacctt tttgccctaa tgatggatta ggctgaaatg
    17221 aactaagcgt agtgggaaaa tctgttgaat aagtgctacc agctatgtaa acttcattat
    17281 tgctacccaa gattatctca cctctagcat catctgcata attgtgcaat agagcaattg
    17341 cagcgtttag tccatcatta ccagtaccac ctaaataggt agatgctatc agtgaagtac
    17401 catcagccga aaaagcagtt atacctaaat cactaccatt agtataattt attacatgag
    17461 tcaaaatata attagtaccc ccattgaaag tagagtcaaa gcatcctact gtatatggat
    17521 aatctgatga acctgttata gtaagtacat atagctcatt cagatcatta gttatcatac
    17581 tgataggcac ttctgctcct gatccgccta agtaagtact ataaatcaga tatgaacccg
    17641 tagtatcata tttagaaata gcagcatcta cattttcacc ataaatagta tcataagcac
    17701 ctaaagtagt aggataaccc ataccaaaac tattaccgcc agcatataaa aaaccttctc
    17761 tatcataagt agctgtatat ccccaattat cagaagtagc tccagaatat gaacaaaaca
    17821 taatttcagg atctatgtgt aattcacttt ttttgtcata cccttcgggg aaatcaaaag
    17881 aaattgtatt tttgtttatt ttatatcgta ttggtatttc tcctccattc tgaaatgctc
    17941 tgggtgtatt taacctcaat tcgcctacag acgtagttat gataatgtcg gtgctatcca
    18001 actttaattt atttatgcct gatacagtta atttaatatc tttcactttt gccattggca
    18061 atacaaaaaa atctattgat agatttccat ttttgctact aattattaca tctattccat
    18121 cgtataattc tttaagtttt acttttctta taggatgaat acctgttttc caattttctg
    18181 ctttatttcc taaataataa ttatgataaa atggcaaaac atctatctct tcaaaactgg
    18241 gaatattagc attatcaaaa aatatttcaa aagcatgagc ttctactctg aatggtaatg
    18301 gcgatttgtt ataattaggg gggcacattt ttctatgata aatagaatca tggataccag
    18361 tttctaaaat attaaataaa atacttttat ctttaatata tatttcattt aaatctactt
    18421 ttactttata tctaaaatcg ttttgccatt gacctttatt tggtataaaa ttaaattctg
    18481 caaaatcttg tgtctttatt gttgaacaat aaaaaataaa aaatataaat aaagcaaaaa
    18541 tagattttaa attcatatat ttaatttata tctaaagctt ttattcaaag ttacataatt
    18601 tttttttatt aatttaaaat ttttaacaaa caaactacag tatttatttt attaaaatat
    18661 atactgtatt aataaaaaat ataattagca tttatatttt ttgaatgata attgaatatt
    18721 agttgtggat ttagtgcttt tttacattta tacaaataat ttttgtatta gataattata
    18781 tagattaata aagaaatttt attgaaaatt taccaataac ttggtgtgaa ataaaaaata
    18841 attgagcaca tcagtagcta ctatttcttt attatgcctt ttaatctcta ctgtttaaga
    18901 ttaattttat atgtacctaa ttagttgaca tgtagataag acaaaaaaat aaagccacta
    18961 aaaaaatgag tggctttatt ttttatagct taatttgatt ttcagccttt accttgtcca
    19021 gcttttactg gttgagcttg ctcttgtggt tgttgagctt ttggtttttg aggttttgat
    19081 tgttgttgag cttcagcttg agcaatacta tctagtcttg cttgttctat ttgagctaaa
    19141 ctatcagcta ctcgagcact atcagctttt acttgagcta tactatcggc tattcgagcg
    19201 ctatccaatt ttgcttgctc tgcctttttc ttagcattac cacatgatgc taacatcatc
    19261 atgcttgcta ctaatactac tactactaaa tttttcatat ccattttgat tttttatggt
    19321 ttgacttgca aaattataaa aaaaatttta ataaaaaaaa tatttttaca attttttaaa
    19381 taatttaaca aaatatcatt cttgtatttg aattaaatca ttatattttt ctattgcgat
    19441 atctatgata gattgatctc ctttgtaatt attaatcaag tgctcaagtg tagcttttgc
    19501 ttgatataga tctccacggc tcagatacac atctgataaa agaataaatc ccttagctat
    19561 ccaatcttcg taaacagcaa aattttctac taaataaaat attttctgtt ctgcattatt
    19621 tagatttcca ttcatatatt cgatagatgc taagtaaaaa gttgcctcgg ctcctatttc
    19681 ggatttttgt ttggatagag cattacatat atctacacat tcacttattt gttgcatttg
    19741 taaatatatg cgagcttttg tcagattagc taatagctta tcattatcat ccatatctaa
    19801 actttctaat atattattag catgttctat agctttattg tagttatttg taagttcata
    19861 tgcttttagt aaatttctca tagcttctat acggttcatt tttacctgag ctatagtaag
    19921 caattgttca tataagggga tacaagcttc gtattcattt ctattataat aaatacgagc
    19981 tgcttttaac aaagatgatt ctgtaaatct agaattttga gcatttatta catacaaata
    20041 atcttgaagt gcattttcga aattacccaa agcataattt gcctctccac gataaaaatg
    20101 tgcatgtata ctataggcac catcaggaaa tttagttaaa tattgatcaa aagctgttat
    20161 agcagattga taatcattag acataaattt attttctgct gaggtatata ttagttcttc
    20221 ctgttcatcg gcacttacac ttttaccata tttctgagca aatgcaaaaa actcatttat
    20281 cctatctgca tcagtatatg tttcacgtag agctatccaa gcatcccttt cagattcact
    20341 atttttatac ttaattatca attcctctaa tacctgtata gctttgtcat attctttttg
    20401 atttctaagg attaatgctt cttttagcat agctttatta gctaatggat gttgtgggta
    20461 tttagtgtaa acctcatgat aggtttttat agcattagtc tcatctatta attcttgact
    20521 caaggctttc tcatataatg cttctggaat atatggtgaa tttgggaaat ttattattaa
    20581 taaatctaaa taattaatct tttccttcaa ttgcccttta atgcctaata ttttagcata
    20641 agaaagatat gcataatcag cttgtttagg atactcatct attacttttt tatacatttt
    20701 agcggcttca tctatttttt ttgtcaaaaa taacgcatca gcataacgaa gataagcgtc
    20761 atactttata tgcgtaggtt cattatctgc taaagatata aaattattaa aagcatctac
    20821 tgctaaagaa tatttttctg tcatcaagta tgaataacca agattataaa aacctttatt
    20881 atattctggt aaattttttg caggagaata atttacaaac gtctgaaaag tgattaatgc
    20941 tgagtcatat tttgctaaag caaaatatgc ttcacctttc caaaataaag cctttgcttt
    21001 taaagttcta tcagttccgt aattaaatgc tttgtttaaa taactaatag atctattgta
    21061 ttgtctgtct ttgaaatcta ataatgcttg atttaagcat gccttctgat acatattttc
    21121 tatagttata ttttttctag gcatctgttc tattatttcg ataacacgag gataattgtt
    21181 agaaatcatt aataaattta acattagctc ttgtgcctca ttttttctaa tagattgtgg
    21241 atactcttcg atgtattttt gtaaagcatt gatagccaaa ttataaggat cataatctaa
    21301 ctcatatgat agctttgcat aattaaaaag agcatcttcg cgtattaaag aatcgaaatc
    21361 aagtgaataa gcatcataaa aagccttttg tgcaaagatt ttttgatctg tttttaaata
    21421 tgcatttcct aaatgatatg ctgcactttg atatatagaa tctttaatgg tcatcacttt
    21481 agtgagatat tgtattgctg acttgtaatc acctgtagaa taataaagat aacctagcat
    21541 ataattatct tctcttgtaa ccttgcctgg tactaattga aaatatgaaa ccaagtgttc
    21601 cttggctaga tttaactcat tttttgtcca ataagcttga gctaacatac gatgtatatc
    21661 tggtaaaaaa gtgacaagag cgttaggaag cattttttcg ccatgtacaa ttacactatc
    21721 tacctgatta gttaaatatt ctaaaaataa aatatgataa tcagtaatat ttaataaagc
    21781 agcatcatca gtatattgag gtattttttt gaattctttt aatgatacat gataattacc
    21841 atttgcataa ttaatataag ctataaaata ttgtgcaaaa ggtgtatacc acgtgtcata
    21901 tctcatcaca ttagacaaat ctactaatgc ttcattatat tgttcagttg taaaatatga
    21961 ataacccctt ttaaaaaata attctgctac taaactatcc accatattat ctatatcaac
    22021 ttcattataa tattcaatag attttttata attttttttc caaaaataat tattccctat
    22081 aaaaaacttg gctttattag tgtatacatg aagaggataa ttgtcaagat aatctaatag
    22141 taacgaaata gcatcatcat tatgcaacat atatgcacta tatgccttgt aataagcata
    22201 ctccgcctct gtggggtaac ttttatcagg tataatctta gtttgttgtt tcaaaatact
    22261 aaattgcttt ttattaaaaa aatcaattac ttgatcatta gtattttgag gtgtaaaaaa
    22321 ataatacgtt tgctgtgaaa aaataacatt aacagaaaaa ataataatca atgtgaaata
    22381 aagtttaatc aaatttttaa ttttcatatt aaaatgaatt tattgcaaat tacttaaaaa
    22441 aatttcaatt gaaaaataaa aaattctaaa acaaacatta tgtttgaaaa gctactaact
    22501 gatgctcaga atatatttgt attggttgat ctaatataaa acaagccgtt gcttttttaa
    22561 aattcgaatt tagtttgttg tttatttatt taatattatg tcaattatgt caacctaaat
    22621 tatatgtaga tttataatta ttaaataggt ttattagctt tgtaatatca tctggtaatt
    22681 cactatcaaa tactatttct tgattagtca taggatgtat aaatccaagt gttttagcat
    22741 gcaaagcttg tcgagaacat attttgaaag aatttaaaat aaattgttta taattggaac
    22801 catatcccga gtatacaatt ttattaccac cataatcttc atcattaaaa attggatgac
    22861 cgatagcttt catatgagca cgtatttgat gggtacgccc agtatataat ttacattcta
    22921 tcaaagtggc aaaaccataa cgttctatta ccctataatt agtatggcta tatttaccaa
    22981 tatttgcgtt tttgctaagt gacattagtc tcctatcttt tttgtcacga gataaataac
    23041 ctataatctc acctgtatca ttttctacat tcccccatac tacagttata tatatccttt
    23101 ttacagaatg ttcaaaaaat tgtttcgaaa taatttgttg agcatactca tttttagcaa
    23161 ccactagaag tccactggta tttttatcta tcctatgaag caaaagagga aattgatcag
    23221 tttttaaaat attttgaaaa taataaagca acccattaag catcgttcca gtataattat
    23281 tatatcctgg gtgaactacc atgccagcag gcttatttat tataataata tcctcatcct
    23341 catataacaa agataaatct attttttctg gtaaaacttc gatgggaatt ggaggatgag
    23401 gtaacatcac acgtatgata tctcctggct ttactttata attagatttc ttagggacat
    23461 catttactaa aatacaacct gacatagctg cattttgtat ttttgttctg ctaatatttt
    23521 ctattttttg ggttagaaat atatctaaac gcatgagaga ttgtcctgga tctacaatta
    23581 ttttgtaatg ttcataaaac tcatcattta attccaatat ttcattatta tcaagcataa
    23641 gttttattgc aaaattattt aatatataca aattaaaaat tttaatattt ttttaaaaaa
    23701 tagttatttt tttgtaacaa tctaaaattt gcttacccaa attttaacct aagatatgtt
    23761 ggatgtggag atttggaaaa ctaatatatg tttcaattaa catatgttat att
//
LOCUS       DAOPRN010000029        22759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1877_length_22759_cov_99.746653, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000029 DAOPRN010000000
VERSION     DAOPRN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1877_length_22759_cov_99.746653"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1640
                     /locus_tag="PKI65_04745"
     CDS             264..1640
                     /locus_tag="PKI65_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOB77782.1"
                     /translation="MKTIWSYLIILMFTTSLMAQNILSTSEFEIIKKNAYKDFHPAIQ
                     SAMIENSIKKLSLNPDLRSQPDPYFKYQIKTGKPTDQKRSGRCWMFASMNTLRPFVID
                     KYNLSNFEFSYVYLFFYDQLEKSNLFLEGIIATVDKPMDDKQVEWWFKNPITDGGVWS
                     SFVNLVNKYGLVPADAMPETYHSNNTREVSSIIATKLREHGIMMREAYAKNKKVDLQK
                     MKTTALSEIYRILVYAFGEPPNTFTYRFVDKNNKPTELKTYTPISFYNEIININFDDF
                     VMLMDDPTRPYYKVYEIEYDRNVVEGINWKYLNLPIDEIKASAMLSLKDSMAMYFSCD
                     VGKQLNNDIGLLDINNYMYDTLFNLPFRMNKKQRIITFESGSAHGMTLIGVDTDINDK
                     PTRWLIENSWGNAGYNGKLIMTDEWMDEYLFRLVVHKKYLPETTLNLLKQKPILLPSW
                     DPMFKMDD"
     gene            complement(2135..4228)
                     /locus_tag="PKI65_04750"
     CDS             complement(2135..4228)
                     /locus_tag="PKI65_04750"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOB77783.1"
                     /translation="MKKLSFILSVIILSTISCKQSKNPLLEEWDTPFQTPPFDKINAG
                     HFKPAIIKAMEIHNKEIDKIIKDKNEPTFENCIVKLDNSGEKLYEITLILFNMIEANS
                     NDTLEQIAQEISPMLSKHNDEILLNEKLFEKIKILYDDKDDLELNDEQKRTLELYYKD
                     FVRAGALLNAEKKEKLKDINQQLSVLTLNFGLNVQKDNNNNLLVIENERDLIGMPENL
                     IATGAKIAEERDVKGKWAFTTDKAVMIPFLQYCPKRELRKKLYDMYLMRGDNDNENDN
                     KKLIQEIVTLRLQKAKLLGYETYADYVLEERMAKTSSNVYDLLRQIWAYSLVKAIEER
                     DEMQKIVIAEGDTFKLKYYDWWYYAEKIRNEKYALDEDKLKPYFSLDAVRDAVFTLSN
                     KLYGISFKKLDNIPVYHNDVEVYEVLDNDKQHLAILYVDYYTRANKQGGAWATGFRDQ
                     YYKEGKNIYPLVSIVFNFPQPIGEHPSLLSMDEVETFFHEFGHALHAFFSNVTYKRLS
                     GYVPRDFVELPSQIMEHWAFEPEMLKLYAKHYKTGEIIPDSLIKKINNSKYFNQGFAK
                     TEFLAAAFLDMDYHTLKQINDLDIHSFEQASMDKIALIPEIKPRYRSTYFNHIFSGGY
                     AAGYYSYIWSEVLDCDAYQSFVETGDIFNKDVADRFRKTILAPGGKKEAEQLYIDFRG
                     KQPDIKYLLKDRGFI"
     gene            4423..>6184
                     /locus_tag="PKI65_04755"
     CDS             4423..>6184
                     /locus_tag="PKI65_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOB77784.1"
                     /translation="MEDSSIQITNKFISDDGFIEAIILSSDLNDTNFDSWLAKYIEET
                     HSRLLKENSEKKLRASACGNLDFENGNFTGWTCQTGKNNGYPAGNWTGSAPVANRHTI
                     ETGGTDPYGGFPKVAPGGGTYSVRLGNNGTGAQAERLIFSFIVGPEDTNFIYKYAVVF
                     EDPGHPENEQPYFELQILNSQNQIISCGQQHYTAASNIPGFINAGGMVWYKPWTTVGI
                     RLANYVGQPVTVIVTNADCSRSGHFGYGYVDFICPHDLVAQANTFCENDSSATLQVPN
                     ISPGATYLWSTGETTSTININPQNFNDSVIQCYVTPPDVPVGCGFWYTFPIKILHIDP
                     HFSFTTSCLNAEFYDSTLINYGNVTYWNWNFGDGNTASIQNPTHAFPATGTYNVTLTS
                     GTPQCQNSITYPLTVTDFDLNISKIDVTCFEYTDGQATAIINGGTAPFSFQWSNGQNT
                     QSISNLSVGTYSVTVSDNNGCMHTASVTINQPPQLVITANANPVLICQGDITNLSVSG
                     ASTYNWSNGLGNGITVTASPTITTTYTVTGSDTIGCTATTEVTVSVNPTPDISPTSDQ
                     TLCHNTQTTAINFTSSVPNTV"
     assembly_gap    6185..6284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6285..8272
                     /locus_tag="PKI65_04760"
     CDS             <6285..8272
                     /locus_tag="PKI65_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOB77785.1"
                     /translation="NGLTCTGQSDTFLIIVNPTPDISPTSDQTLCHNTQTTAINFTSS
                     VPNTVFNWINNNSSIGLAANGTGDISSFTVTNTGTAPQTSTIIATPSFTNNGLTCTGQ
                     SDTFLIIVNPVPALTMAQTDTVICYGKSVLISVSGAMTYQWSNNLGNNSTILVTPDAN
                     STYSVTGTNQYGCTSSVSASIVVNPVPDVGINPDSIEICDNTTITLVASGANTYLWAG
                     GLGANASITITPHNTTTYVVTGTNQYGCTSSARSKVIVHPLPVVTINPNNSEICLGDT
                     ISLVANGAQTYLWSGGLGTNNSLSVSPQVSTTYNVTGTNQWGCTASVSANIIVHPLPN
                     LTISPHNISICYGESTTLTANGAHTYQWSNGSSNQSITVEPLFSTTYYLTGYSIYECV
                     KIDSAQVMVNPKPIIDFIAEPLSGCEPLSVKFINNSDNGTYYWYFGDGNSSNIYSPIH
                     IYNNDGVYTVTLIVKNQYNCSDTLTKFNYITIYPSPIAGFSIFPLITTEDDGRVKILD
                     ESYGATSWYYDFGEENEQNDIFTDREPNYIYTNAGNYTIIQIVANEYNCYDTAYNNVI
                     IKPSTMFYIANSFTPNADGLNETFMPLGFNVPIKDYEFRIFDRWGKQLFYTTDLYVGW
                     DGKYKDEFVKQDMYVYSIKVILEDGEHIFRGALYVIY"
     gene            8583..10535
                     /locus_tag="PKI65_04765"
     CDS             8583..10535
                     /locus_tag="PKI65_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOB77786.1"
                     /translation="MGYLSFDKQQLVNLEFSLNREIIRANRAGSYASTTIIYCNTRKY
                     HGLLISPQPQLDDDLHVFLSTLDESIIQHGSEFNIGIHRYPGGIYSPKGHKYVEEFHS
                     MPIPTLIYHVGGVILSKQLLFINKEDRVLVKYTLLDAHSETFLRLKPFLAFRNYHTLS
                     KANMYANKNYEECENGIIMKLYQGYSPLYIQISKESKYVHAPDWYYNIEYTKELERGY
                     DGHEDLLVPGYFEFPIKKGESVIISAGLSETKTNSLKRLFTNELKNRIPRDSYENNLI
                     NSAQQFIIQRGNEVEIKAGYHWFGRWGRDTLISLPGLTLVTGNYDLCKKAIDTLLADK
                     KGPLLPNVGKKQRVAINSADTSLWLFWTIQQYQLFTNETPMKIWRYFKDDLISILYGY
                     RNGEAKGIWMSDDGLINAKLPGYALTWMDAIVDGKPVTPRYGKAVEINALWYNAICYT
                     IYLAEHVNDEEFINDWLPIKEKVELSFVNNFWDEERGYLADFVDNDEIDWCVRPNMTF
                     ATSLPYVCIDEKKRLSILERIQQELLTPRGLRTLSPKCPNYRGLYEGPIRERDLAYHQ
                     GTVWPWLLGAFAEGYLRIHGKAGLPFIEKIYYDLEPTLAEHGIGSISEVFDGDPPHKP
                     GGAISQAWSVAEILRIKWLIEKYKNL"
     gene            10532..11827
                     /locus_tag="PKI65_04770"
     CDS             10532..11827
                     /locus_tag="PKI65_04770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOB77787.1"
                     /translation="MRVLMFGWEFPPHISGGLGTACYGLTKALLRHGVDVIFVVPKAF
                     GDEDNEIKILNASDFEINLEEETSIELWKRLKYIQVASKIIPYISPEAYTEEYERLRK
                     EEIKKNDFFKRKYEFSGSYGPNIMYEVSRYALIASAIAATHDFDVIHAHDWLTYPAGI
                     AAKSTSGKPLIVHVHATEFDRSGENINQDVYNIEREGMLKADKVVTVSNYTRSIVINR
                     YGIEPDKVVTVYNGADAFKFHSRPKVDKPYKEKVVTYIGRITFQKGPEYFVETARKLL
                     KYDDNVRFVMAGSGDMFPRIVKLAARYRIGHKIHFTGFLKGPEVEEVLSYSDVYVMPS
                     VSEPFGISPLEALMSNVPIVISKQSGVSEVLKHAIKVDFWDIDAMANAIYGILHYPAI
                     SKMFQKYGKAEVDQLKWDNSAYEVKQLYESLLSEYLILK"
     gene            11835..13022
                     /locus_tag="PKI65_04775"
                     /pseudo
     CDS             11835..13022
                     /locus_tag="PKI65_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
     gene            complement(13589..14230)
                     /locus_tag="PKI65_04780"
     CDS             complement(13589..14230)
                     /locus_tag="PKI65_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOB77788.1"
                     /translation="MYKIFIKNKIILITDNIQILKHINEKVLIKYQPDLNEIIDLLKQ
                     FADDDLHQQLILINYKLEDFYIKFYNLFKVVLGAGGVVFNEEKDKILFILRNGVWDLP
                     KGKIDYGESDEKAAIREVCEETGVCDLTLVKRLEPTYHIYFSNNEAYLKETHWFSMKA
                     KEGQPLIPQKEEKIEMVKWFTQDDIKNNWDNMYLSIKSLLVENKFFMLDIDSP"
     gene            14283..14891
                     /gene="pyrE"
                     /locus_tag="PKI65_04785"
     CDS             14283..14891
                     /gene="pyrE"
                     /locus_tag="PKI65_04785"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOB77789.1"
                     /translation="MQIKAIKLNYTNPFTWTSGIKSPIYCDNRIILSYPDHRTEIANF
                     LKEIVIKKFSDADYIAGVATAGIPMGTLIAHILNKPFIYVRPKAKEHGLHNQIEGFLP
                     ENKKVVVVEDLISTGKSSKNAISAIREANCEVIGLISIFSYAFSKVENDFNNNGIKVF
                     SLADYPNLLPIAIANKYIPYDVLHILEQWHLDPKNWYSNFFE"
     gene            15021..15887
                     /locus_tag="PKI65_04790"
     CDS             15021..15887
                     /locus_tag="PKI65_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOB77790.1"
                     /translation="MEKSELVLQPDGSVYHLALFPHEIADTIILVGDPERSDWVSSFF
                     DKIEVSRRNREIISYTGYYKNKRISVVSTGMGTDNIDIVVNELDALANIDLEKREVKR
                     EHKSLNLIRIGTSGALHNKIDINNVVISEMALGMDGLLHYYKHDSSIRETDLEKAVLQ
                     KVNWPDTLPKPYIVSGDQGLFNKLNSDQFHKGITVTASGFYAPQGRVLRLPLAYPELI
                     NQFENFEYQGHILANFEMETSALFGLAKLMGHRACTLCVAIANRVTKDFSEDYSQAIK
                     NLALHVLDKMTE"
     gene            15962..16309
                     /locus_tag="PKI65_04795"
     CDS             15962..16309
                     /locus_tag="PKI65_04795"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOB77791.1"
                     /translation="MKREEVYSKAVKAGRRTYFFDVKENNSGDRYITITESRKELDEV
                     TGQFGYKKSKIFLYKEDMDKFARGLRYAIEFIQTGVDPEEGKEEILNSHKSDGQTEDF
                     NKLEESDNEDIIF"
     gene            16314..17102
                     /locus_tag="PKI65_04800"
     CDS             16314..17102
                     /locus_tag="PKI65_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOB77792.1"
                     /translation="MGKVIAIANQKGGVGKTTTALNLGGAFAALDLKTLIIDADPQAN
                     STSGLGIDPKAVDKSIYECIISNEAPDKAIVDTSTPNLFLLPAHINLVGAEIELINME
                     NREKKMKTVVDIIKNEYEIILIDCSPSLGIITINALTAADSVIIPVQCEYFALEGLGK
                     LLNTIRIVQSRLNPNLDIEGILLTMYDRRLRLANQVVDEVRHHFKDIVFDTIIPRNTR
                     IGEAPSYGETIITFDTTGRGAQSYIALAKEILNKSKKENVNVSK"
     gene            17089..17955
                     /locus_tag="PKI65_04805"
     CDS             17089..17955
                     /locus_tag="PKI65_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOB77793.1"
                     /translation="MSASKRPALGRGLEAILGDIDSDYFTSPAFPLIPIDQIESNPYQ
                     PRTDFDEDNLYELAESIKHLGLIQPITVRKIDDKKYQLISGERRLRAAKLANLTEIPA
                     FVKDADDDALLEMALVENIQRQDLNAIEIANSYQKLIEEFDLTQEELSSRVGKNRATI
                     SNYLRLLNLPPAIQAAIRYDKISMGHARALASIEDEKDLMTIFKKIIDEELSVRKVEQ
                     LVSNLKNKKTDNHPTQCQISQKAENIIKNIKEKYNIEAKSKLTTKGKVVVQLKFNNDE
                     EMEDFFNIFLNE"
     gene            17948..18559
                     /locus_tag="PKI65_04810"
     CDS             17948..18559
                     /locus_tag="PKI65_04810"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOB77794.1"
                     /translation="MNKYIFIFIISISLCININSQNLITGYSFDSYRLNYSYSQKNDS
                     IHSPKKAALYSSLLPGLGQGYNKKFWKIPIIYAGLGGSAYAFFYNRKYYVDYRDAIEM
                     RLDDDPNTIDPYIDKYSLEQLSYYKDFYQRNIEISAIVFVCVYLLNILDAIVDAHLFD
                     FSINTKLSYLPVYNNTFIIPTVSINYNFFKKNKNDKISYNRIW"
     gene            18528..19262
                     /gene="dapB"
                     /locus_tag="PKI65_04815"
     CDS             18528..19262
                     /gene="dapB"
                     /locus_tag="PKI65_04815"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOB77795.1"
                     /translation="MIKLAIIGYGKMGKEIENCAKDLDFSIACIIDKDNNLRNDIIRY
                     KPNVAIEFTNADAVVNNIKTCVDMNLPVVSGTTGWDKNIDEVKNYTIRNNGNVLWGSN
                     FNIGVNLWFDFLNELACIMKNYDLYKVELEEIHHIHKLDKPSGTAIQAANILMKQYPI
                     LKNWSLDHTEDTLHINSIRQGEEVGTHRVIFKSPYDQISFEHKSFSRKALAVGALAGA
                     KWIIDNAGFFIFHDVYQSVFEKFNKA"
     gene            19480..20154
                     /locus_tag="PKI65_04820"
     CDS             19480..20154
                     /locus_tag="PKI65_04820"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOB77796.1"
                     /translation="MKYFFIIIFFLIATYGFAQNEYNRLIEFAGIVVTADSLRPIPFV
                     HIFVKDSRRGTVSDINGLFSIVGEKGQTIVFSAVGYKTVFFKIPDTLKHTFYNLIQPM
                     ITDTIYLDKTVVYPWPSKEKFKEAFINTVPPDDDLERARKNLAFMEWKDKEFNYVARD
                     GQSAYNQMIKEISYKNYYAGQLPPNNLLNPFAWAAFVKAWQEGQFKRNSQPNKSQEYY
                     YNEPLH"
     gene            20138..22654
                     /locus_tag="PKI65_04825"
     CDS             20138..22654
                     /locus_tag="PKI65_04825"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOB77797.1"
                     /translation="MNHYINILFLLLFLNFSLYCQENIIINGYIKNQNNEPIPYANIG
                     IVGTNLGTASHQNGFFEISVPISDTVIIGISTLGYERLYDTLIKPIKNITKTYILKTT
                     SMLLPQVEITEQVDIAKGIQRLDPKKAELLPTPGGQVETLIKLMGMGVQSSNELSSQY
                     SVRGGNYDENLVYINDFEIYRPLLTHSSEQEGLSFLNSDLISSIDFSSGGFEAKYGDK
                     MSSVLDATYIRPSDRLTTIQASLLSANISTMGTLLKNKMTYLAGFRYKSNKYLLSTLP
                     TTGDYFPKIFDFQTYLTYQFNDRSELAALGYWNFNQFTFYPKSRETSFGTISEAYKIN
                     MYFEGVEKNLYNIWMGGLKWTYITDSNTIFKTIISTYYSNEQEIYDVLSQYWLHTLEI
                     DFESETFGRQGELMGIGSYLNHGRNYLDLQVINLEGKINHKITKHNIDLGIKGQGNWF
                     DFKMREWIYMDSAGYSLPNIHDSIGYTQPQPYQILNLYEFLHNKKTINTWHITGYIQD
                     MWSKKFIDKGTITTILGFRTGYYSINNTFMFSPRFSISYTPEWQRLMAFRLSLGLYQQ
                     PPFWKEMVGWDGTIYDNVPNQKSFHFVLGMEYSFLAWNRPFKFITEAYYKYLYNLVPY
                     VVDNIRIRYLPEQRSKAYATGIDLKLYGEFVPDVDSWISVSIMKTAEDIIGDYYYIYY
                     NSDGERIIPNYISNNIPVDSVKISPGYIPRPGDQRINISLFFQDYVPNNPNFKMSLAL
                     QFGSGLPFGPPGNKKYLHINRTSPYRRVDIGFSAKLFTGDSKIAKTLKECWLSLEVLN
                     LLQIRNVASYVWVRDVTDRMYAVPNYLTDRQLNLKLIVKF"
BASE COUNT         8036 a         3219 c         3685 g         7719 t
ORIGIN      
        1 tagataaact acttcaagta aaacaatgat ttgtttctga acccctttcg attccaccaa
       61 gtaatatttt agctagctaa aatattacct aacctttata atactccttt ttacaactct
      121 attaaaatta catattgcta gagaaatata tttgtgcaga aacacgtgga tgatgatatt
      181 ataacttttt aagtatgaaa tttagttaca aataagttaa tttattaaaa atacttatta
      241 tctttgttaa aaaaaatatg aagatgaaaa caatttggag ttatttaatt attttaatgt
      301 ttacaacgag tttgatggca caaaacattt taagtacgag tgaatttgaa ataattaaga
      361 aaaatgcata caaggatttt cacccagcta ttcagtctgc tatgattgaa aattcaatta
      421 aaaaattatc actaaaccct gacttacgta gccagccaga tccatatttt aaatatcaaa
      481 taaaaacagg taaacctaca gatcaaaaac gctctggtag atgttggatg tttgctagta
      541 tgaatacgtt acgtcctttt gtaatagata aatataattt atctaatttc gaattttctt
      601 atgtatattt atttttctat gatcaattag aaaaatcaaa tttattttta gagggtataa
      661 tagctactgt agataagccc atggatgata aacaggtgga atggtggttt aaaaatccta
      721 tcactgatgg cggtgtatgg tcaagttttg taaatttagt caataaatat ggattagtac
      781 cagcagatgc tatgcccgaa acatatcaca gcaataatac tcgtgaagtt tcttctatca
      841 ttgctaccaa actcagagaa cacggcataa tgatgcggga agcttatgct aaaaacaaaa
      901 aagtagattt acaaaaaatg aaaacaacag ctttaagtga gatttatcgc atattagttt
      961 atgcatttgg tgagccacct aatactttta cttatagatt tgtggataaa aacaataaac
     1021 ctactgagct aaaaacttat actcctattt ctttttacaa tgaaataatt aatataaatt
     1081 ttgatgattt tgtaatgctg atggatgatc ctacaagacc ttattacaaa gtatatgaaa
     1141 tagaatatga tagaaatgtg gtggagggaa ttaactggaa atatctaaat ctgcctatag
     1201 atgaaataaa agctagtgct atgctatcgc ttaaagattc aatggctatg tatttttctt
     1261 gtgatgtagg taagcaatta aacaatgata taggcttgtt ggatattaat aattatatgt
     1321 atgatacatt atttaacttg ccatttagaa tgaataaaaa gcaaagaatt attacattcg
     1381 aaagtggctc tgctcatggt atgactttaa taggtgtaga tacagatatc aatgataagc
     1441 cgactcgctg gttaatagaa aacagttggg gtaatgctgg ttataatggt aaactcatta
     1501 tgactgatga atggatggat gaatatttat ttcgcttagt agttcacaaa aaatatttac
     1561 cagagactac cctaaatcta ttaaaacaaa aaccaatcct tttgccaagc tgggacccta
     1621 tgtttaaaat ggatgattag ttttttttta actaataact tacaactttt ttatttttct
     1681 aaaatttttg tcttttcttt gttttatgtg tttggagtat ctagttttta atattaaatt
     1741 atatagatcc agtataatta aacaagaaat gatttaaagt ttttttaatt aaaaaaaata
     1801 atgtaggtta tttaaatttt aatgaaataa ccccactcac taaagttatt tttggttcta
     1861 taacgtttat tgtggataaa ttatttaggt tgattttttt accacaaagg gcaccaagtt
     1921 attacacaaa gtccacaaag tcccatttgt agccctttta attccatcct tctctgtgtt
     1981 ctttgtgcat cctttgagaa ctttgtggtt aatatttaac tgtcctataa taaaaaacta
     2041 atattaacaa attcattttt gtaaaattac tcttttttta atctttaacc catataaaac
     2101 gttatagaac cattattttt taagaattaa aaaatcaaat aaaacctcta tcttttaaaa
     2161 gatatttaat atcaggttgt ttgccacgaa aatctatata cagctgttcg gcttcctttt
     2221 taccaccagg agcaagtatg gtttttctaa atctatcagc gacatcctta ttgaatatat
     2281 caccagtttc tacaaaagat tgataagcat cacaatctag cacttcactc catatatagc
     2341 tgtaatatcc cgcagcatat ccacctgaaa aaatatgatt aaaatatgta gaacgataac
     2401 gaggttttat ctcaggtatg agagcgattt tatccattga tgcttgctca aaagaatgta
     2461 tatccaaatc atttatttgt ttcaaagtat gatagtccat atctagaaat gccgcagcta
     2521 agaattctgt tttagcaaaa ccttgattaa aatatttcga attattaatc tttttaatca
     2581 aactatcagg tattatttct cccgttttat aatgtttagc ataaagttta agcatttctg
     2641 gttcgaaagc ccaatgctcc attatctgag agggtagttc tacaaaatct cttggtacat
     2701 aaccagataa tctcttgtaa gtaacattag aaaagaacgc atgtagagca tgaccaaact
     2761 catgaaagaa tgtttctact tcatccatag acaacagact aggatgttca ccaatgggtt
     2821 gtggaaaatt gaatactata ctgacaagtg gataaatatt tttcccttct ttgtaatatt
     2881 gatctctgaa tcctgtagcc catgcaccac cttgcttatt agcacgagtg tagtagtcta
     2941 catacaatat cgctaaatgt tgtttatcat tatccaaaac ttcatacact tctacgtcat
     3001 tatgataaac tggaatattg tcaagttttt taaaagatat gccataaagt ttattggata
     3061 aagtaaatac tgcatcacgt acagcatcta aactaaaata tggttttaat ttatcttcat
     3121 ctaatgcata tttttcatta cgaatttttt cagcataata ccaccaatca taatatttga
     3181 gcttaaaagt atctccctcg gcaataacaa ttttttgcat ttcgtcacgt tcttcaatag
     3241 cttttaccaa actatatgcc catatttgac gcaataaatc ataaacatta gaagaagttt
     3301 tagccattct ctcttctaat acataatctg cataagtttc atatccaagt agtttagctt
     3361 tttgcaggcg taatgttacg atttcttgaa tcaacttttt attgtcattt tcattatcat
     3421 tatctccacg catcaggtac atatcataaa gttttttacg aagttcacgt ttagggcaat
     3481 attgaaggaa tggtatcatt actgctttgt ctgtagtgaa agcccattta ccctttacat
     3541 cacgttcttc agctatttta gcaccagtag cgattaaatt ttctggcatg cctattagat
     3601 ctctttcatt ttctattact aatagattat tattattatc tttttgtaca tttaaaccaa
     3661 aatttaatgt taatacagat aattgttgat taatatcttt taatttttct tttttttcag
     3721 catttaataa ggcaccagcc ctcacgaaat ctttgtaata aagttctagc gttctttttt
     3781 gttcatcatt taattctaga tcatccttat cgtcatatag tattttaatt ttttcaaaaa
     3841 gcttttcatt taataaaatc tcatcattat gcttagaaag cattggtgaa atttcttgag
     3901 ctatttgttc taaggtgtca ttagaatttg cttctatcat attaaaaagt attaaggtga
     3961 tctcatataa cttttcacct gaattatcta attttactat acaattttca aaagtaggtt
     4021 catttttgtc ttttattatt ttatcgattt ctttattatg gatctccatt gccttaatga
     4081 tagcaggttt gaaatgccct gcatttattt tatcaaaagg gggtgtttga aaaggcgtat
     4141 cccattcttc taatagagga tttttgcttt gtttacaact tatagtactt aaaattatta
     4201 cactcaaaat aaatgataat tttttcatat ttttctacaa aaataattat tatttttgaa
     4261 tattattttt taatttagta aaacaaaata aatttatttt taaatatatt aaacttttta
     4321 gaaaatatat gtctaattta tataaataaa tatgttatga aaaattattt atcattttta
     4381 atattaattt ttttcacagt tagtttattt tctcaagaaa atatggagga ttctagtata
     4441 caaatcacta ataaatttat ttcagatgat ggttttattg aggcaataat attatcaagt
     4501 gatttgaatg atactaattt tgatagctgg ttagctaaat atatagaaga aactcacagt
     4561 agactattaa aagaaaactc ggaaaaaaaa ctaagggcga gtgcttgtgg taatttagat
     4621 tttgagaatg gcaatttcac aggttggaca tgccagacag gtaaaaataa tggttatcca
     4681 gcaggtaatt ggacgggtag tgcacctgtg gctaataggc acacaataga gacgggaggt
     4741 acagatcctt atggtggttt tccaaaagtg gctcctggtg gaggcacata ttcagtacgt
     4801 ctcggaaata atggaactgg tgctcaggct gagagattaa ttttttcatt tattgttgga
     4861 ccagaagata caaattttat ttataaatat gctgtggtat ttgaagatcc tggacatcca
     4921 gaaaatgaac aaccctattt cgaattacaa atattaaatt cacaaaatca aattatttca
     4981 tgtggtcagc aacattatac tgcagcaagt aatatacctg gatttataaa tgctggaggc
     5041 atggtatggt ataagccatg gacaacagtg ggtattagat tagctaatta tgttggtcaa
     5101 ccagtaacag taatagtaac aaatgcagat tgttctcgta gtggacattt tggttatggt
     5161 tatgtcgatt tcatttgccc tcatgattta gtagctcaag caaacacatt ttgtgaaaat
     5221 gattcatcag ctacacttca agttcctaat attagtccag gagctacata tctatggtcc
     5281 actggcgaaa caacatctac tataaatata aatccacaaa acttcaatga ttcagtgata
     5341 cagtgctatg taacacctcc tgatgtgcca gtaggttgcg gattttggta cacttttcct
     5401 attaaaatat tacacataga tcctcatttt agctttacta ctagttgctt aaatgctgaa
     5461 ttttatgata gtacattaat taattacggt aatgttactt attggaattg gaactttggc
     5521 gatggtaata ctgctagtat acagaatcct actcacgctt ttcctgctac tgggacctat
     5581 aatgtaactc taacaagtgg tacgccacaa tgtcaaaatt caatcacata tcctttaact
     5641 gtaacagatt ttgatcttaa tatttctaaa attgatgtca cttgttttga gtatacagat
     5701 gggcaagcta cagcaattat taatggaggc actgctccat tttcatttca atggtctaat
     5761 ggacaaaata cgcaaagtat ttctaattta agtgtgggca cttattcagt tacagtatca
     5821 gataataatg gttgtatgca tactgcaagt gtaaccataa atcagccacc acaactagtg
     5881 ataacagcaa atgccaatcc agttttaatt tgtcagggtg atataacaaa tttatctgtt
     5941 tcgggggctt caacttataa ttggtctaat ggattaggta atggaataac agtgacagct
     6001 tctccaacta taactactac atatactgtt acaggttcag atactattgg ttgtacagca
     6061 acgacagaag ttactgtctc agtaaatcct acaccagaca tatctcctac ctcagatcag
     6121 actttatgtc acaatactca aacaacagcg ataaatttca ctagttctgt tcccaataca
     6181 gtatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnataatg gattgacatg
     6301 tacgggccaa tcagatacat ttttaattat tgtaaatcct acaccagaca tatctcctac
     6361 ctcagatcag actttatgcc acaatactca aacaacagcg ataaatttca ctagttctgt
     6421 tcccaataca gtatttaatt ggattaacaa taattcaagt ataggattag cagctaatgg
     6481 cactggtgat atatcatctt ttactgttac taatacaggt acagcacctc aaacctctac
     6541 aataatagca acaccatcat ttactaataa tggattgaca tgtacgggcc aatcagatac
     6601 atttttaatt attgtaaatc cagtgcctgc tttgactatg gcccaaacag atacagttat
     6661 atgttatgga aaaagtgttt taatctctgt aagcggagcg atgacttatc aatggtccaa
     6721 taatttaggt aataactcta caattttagt tactcctgat gctaatagca cttatagtgt
     6781 aacaggcaca aatcaatatg gttgtactag ttcagtgagt gcctccatag ttgtaaaccc
     6841 ggtacctgat gtaggcatca atccagattc tattgaaata tgtgataata ctacaattac
     6901 tttagtagca tcaggtgcaa atacctattt atgggcaggc gggttaggtg ctaatgcctc
     6961 aataaccata acacctcata atacaactac ttatgttgtt acaggtacaa atcaatacgg
     7021 ttgtactagt tctgctaggt ccaaagtaat cgtgcatcct ttgccagtag ttacaatcaa
     7081 tcctaataat agtgaaattt gcttaggaga tactatctca ttagtagcta atggagctca
     7141 aacgtattta tggagtggag ggctaggtac taataattct ctttcagtat caccacaagt
     7201 tagtacaacg tataatgtaa ctgggactaa tcagtggggt tgcactgcat ccgtaagtgc
     7261 taatattatt gtgcatcctt tacctaatct cactatatct ccacataata tatctatttg
     7321 ctatggagaa agcaccactc tgacagcaaa tggggctcat acatatcaat ggagcaatgg
     7381 tagtagtaat caatctataa cagtggagcc tctattctct actacatatt atttaacagg
     7441 atacagtatt tatgaatgtg taaaaataga tagtgctcaa gtaatggtaa atcccaaacc
     7501 gattatcgat tttatagctg aaccactaag tggttgtgag ccattaagtg tgaaatttat
     7561 taataatagc gataatggaa catattactg gtattttggt gatgggaaca gttctaatat
     7621 ttattctcct atacatattt ataataatga tggagtttat actgtaactt taatagtaaa
     7681 aaatcaatat aattgttctg atactttaac aaaatttaat tatataacta tatatccttc
     7741 tcctattgcc ggttttagta tattccctct tattactaca gaggatgatg gtagagtgaa
     7801 aatactagat gaaagctatg gtgctacaag ttggtattat gattttggcg aagagaatga
     7861 gcaaaatgat atttttactg atagggaacc caattatatc tatacaaatg ctggtaatta
     7921 cactataata caaatagttg ccaatgaata taattgctat gatacagctt ataataatgt
     7981 tattataaaa ccatctacaa tgttttacat agctaattca tttaccccta atgctgatgg
     8041 attaaatgaa acttttatgc ctttgggctt taatgtgccg ataaaggatt atgagttcag
     8101 aatctttgat agatggggca aacaactttt ttatactaca gacttatatg taggctggga
     8161 tggtaaatac aaagacgaat ttgtaaaaca agatatgtat gtatattcta ttaaagtaat
     8221 cctagaagat ggtgagcata tttttagagg tgctctatat gtaatatatt gaaattattt
     8281 attaggacta ataaataaac aaatgacctt tgaatagcaa atgtgaaaat aaaaaattat
     8341 gtgttgataa taaatgttat acttatctaa ttgatataaa attgccgaat tttttttaaa
     8401 gtatctgatt atttgagttt tgatttaaaa aaaactaaac aaattttttg caaaaaatta
     8461 taaaatatta tttttttatt taattgataa ttgtgattat tttttagctt tatataattt
     8521 atttattttt ttatttgatg attttttatt aaatttgcat aaaattattt ataatataat
     8581 ttatggggta tttatctttt gataaacaac aactagttaa tttagagttt tcattaaatc
     8641 gagaaataat cagagctaat agggcaggat cgtatgctag tactacaatc atttattgca
     8701 atacgcgtaa atatcatggt ttacttatat ctcctcaacc acaattagat gatgatttgc
     8761 atgttttttt atctacatta gatgaaagta tcatacaaca tggtagtgag tttaatatag
     8821 gcattcacag atatcctggt ggtatttatt ctcctaaagg tcataaatat gtagaagagt
     8881 ttcatagtat gcctattcct acattaattt atcatgtagg tggtgttatt ttatctaaac
     8941 agttattatt tattaataaa gaggacaggg tccttgtcaa atacactctt ttagatgcac
     9001 atagtgagac ttttctgcgt ctcaaaccat tccttgcttt tagaaattat catactctca
     9061 gtaaagctaa tatgtatgca aataaaaatt atgaagaatg cgagaatgga attataatga
     9121 agctatatca aggttatagt ccactttata tacaaatttc taaagagtct aaatatgtac
     9181 atgcaccaga ttggtattat aacatagaat ataccaaaga attagaaaga ggctatgatg
     9241 gacatgagga tttgctggtg cctggatatt ttgaatttcc tatcaagaaa ggtgaaagtg
     9301 tgattatcag tgctggttta tcagaaacta aaacaaactc attaaaaaga ttatttacta
     9361 atgaattaaa aaataggatt cctagagata gttacgaaaa taatttaatt aattctgctc
     9421 aacaatttat tatacaacgt ggtaatgaag tagagataaa ggctgggtat cattggtttg
     9481 gtagatgggg gcgcgataca ttgatttcat tacctggatt aaccttagtg acgggtaatt
     9541 atgatttatg taaaaaagct attgatactt tattagctga taaaaaagga ccattgcttc
     9601 ctaatgttgg taaaaaacag cgagtagcta tcaactcagc agatacttcg ctttggcttt
     9661 tttggactat acagcaatat cagcttttta ctaatgaaac tcctatgaaa atatggcgtt
     9721 atttcaaaga tgatctaata tccattctat atggttatcg taatggcgaa gccaaaggaa
     9781 tatggatgag tgatgatgga ttaattaatg ctaaattacc aggttatgct cttacttgga
     9841 tggatgctat agtagatggt aaacctgtaa cgcctcgtta tggcaaagct gttgagataa
     9901 acgcattatg gtataatgct atatgttaca ctatttatct agcggagcat gtgaatgatg
     9961 aagaatttat taatgattgg ctacctatta aagaaaaagt agagttatca tttgtaaata
    10021 atttttggga tgaggagcgt ggttatctag cagattttgt agacaatgat gagatagatt
    10081 ggtgtgtgag acctaatatg acgtttgcta catctttacc ttatgtttgt atcgatgaga
    10141 aaaaaagact ctctatttta gaaagaattc agcaagaatt attaacgcca agaggtttgc
    10201 gtacacttag cccaaaatgt cctaattatc gtggcctcta tgaaggccct atcagagaaa
    10261 gagatttggc atatcatcaa ggtacggtat ggccttggct tttaggtgct tttgcagaag
    10321 gatatttgag aattcatggt aaagcaggtc tcccatttat agaaaaaatt tattatgatt
    10381 tagaaccaac actagccgaa catgggatag gtagcataag tgaagtgttt gatggtgatc
    10441 caccacataa accaggtggt gctatttccc aagcatggag tgttgcagaa attttaagaa
    10501 ttaaatggtt aatagaaaaa tataaaaact tatgagagtg ctaatgtttg gttgggaatt
    10561 tccccctcat atttctggcg gactcggtac tgcctgttat ggtctaacta aagccttatt
    10621 aaggcatggt gtagatgtta tttttgtcgt tcctaaggca ttcggcgacg aagacaatga
    10681 aataaaaata ttgaatgctt ctgattttga aataaattta gaagaagaaa catcaataga
    10741 actttggaaa agattgaaat atattcaagt agcttctaaa ataatcccat acattagtcc
    10801 agaagcttat acagaagaat acgaaagatt gagaaaagaa gaaatcaaaa aaaatgattt
    10861 ttttaaaaga aaatatgagt tttcaggttc ttatggcccc aacataatgt atgaagtttc
    10921 tagatatgcc ttaatagcaa gtgcaatagc cgctactcat gattttgacg ttatacatgc
    10981 tcacgattgg ttaacctatc cagcgggcat agctgctaag agtacaagtg ggaaaccatt
    11041 aatagtacac gtgcatgcta ctgagtttga ccgtagtggt gaaaatatta atcaagatgt
    11101 atataatatc gagcgcgaag gtatgttaaa agctgataaa gtggtaacag tatctaatta
    11161 tactagaagt attgttatta atagatatgg tatagaacct gataaagtag ttactgtata
    11221 taatggcgct gatgcattca agtttcattc acgccccaag gtagataaac cttataaaga
    11281 aaaagtagta acatatattg gtagaatcac ttttcaaaaa ggtcccgaat actttgttga
    11341 aacagcccga aaacttttaa aatatgatga taatgtcaga tttgttatgg cgggttctgg
    11401 tgatatgttt cctcgtattg ttaaattagc agctcgctat cgcataggac acaagattca
    11461 ttttactggt tttttaaaag gtcccgaggt agaagaagtt ttatcttata gcgatgtata
    11521 tgtgatgcct agcgtttcag aaccttttgg tatttctcca ttagaagctc taatgtctaa
    11581 tgtacctatt gtaatatcta agcaaagtgg agtatcagaa gtattaaagc atgctatcaa
    11641 agtagatttt tgggatatag atgccatggc taacgcaata tacggcatat tacactatcc
    11701 agctattagt aaaatgtttc aaaaatatgg caaggctgag gtagaccaat tgaaatggga
    11761 taactcagcg tatgaagtaa aacaacttta tgaatcttta ttaagtgaat atttaatttt
    11821 aaaatgaaaa aagtatgagg tatatctgtt tctattttca ggttcatcag ccaataagat
    11881 taagaacgta tcgttttttt gatataggta taaatcataa ttattatgat gattatcaaa
    11941 atcaatatat attacgtagg gttgctgata agtgttatct accaatgaat caattacttt
    12001 ttgaacttat ccaagagttt ggacaaagat tcaaagtatc cttttctatt acaggtaccg
    12061 cattagaaca atttgaacgt tatgcacccg aagttatcga aagcttcaaa ttattagcag
    12121 agactggatc ggtagaattt ttagctgaaa cctatagtca ctctttagca tctgttattt
    12181 caaaagatga attttattct caaattaatt tacatcaaga taaattaaaa tcattgatag
    12241 gtgtgaagcc tagtactgct tttagaaata cagagcttat ttatagcgat cagataggtg
    12301 aatatgtagc tgaccttgga tacaaagtta tgcttgctga aggtgctaaa catatattgg
    12361 ggtggaaaag ccctcatttc ctttattata acgttattaa tcccgaacta aagcttttgc
    12421 taaaagatta tcaattaagt gatgatatag cttttagatt ttctaataga cagtgggatc
    12481 agtggccact cactgcagag aaatatgtag actggctcaa tgctattgac agtaagcacg
    12541 aagttgtcaa tttatttatg gattatgaaa cttttggtga acatcaatgg aaagaaactg
    12601 gtattttcga tttcatgcgt gcactcccat atcgtatatt ctctcattca gattatgaat
    12661 ttgcaactcc tacagagctt agtgagcttc tgcagcccgt agccccttta catgtaccgt
    12721 atcccacttc atgggcagac gaagaaagag atttgacagc ttggctaggc aatgaactac
    12781 aagaagatgc ttttaatacg ctatattcac tcagagacaa ggtatcacaa actgatgacc
    12841 ccgatatata tagagattgg ttgtatctac aaacaagtga tcacttttat tatatgtgta
    12901 ctaaatggtt ctctgatgga gatgtacata agtatttcaa tccttatggc tcgccttacg
    12961 atgcttatat taattatatg aatatattga gtgattttat tattagaatt gataaaaaaa
    13021 ttgaagaaaa aacttccttt caagagttga aacatactac ctcagatact aaaactcaaa
    13081 aaactgaaaa atcgacaata acttcgaaaa agaaaaaaac tacccacgat gttcagataa
    13141 caagcgatgg agataacata aaatttacaa aagaacaaat tgataagcta gctgaagaat
    13201 ttaccccgag agaaatcgct attctattta aacagctttc tgacaacact gttaaatcaa
    13261 taaaaaataa attgaataaa gatttaaaaa tcaaagtaga gaaagaattg gctaaaacta
    13321 tgagacctag aaaagaaact attaataaga ttttaaataa atttaatgac ttgatcaaat
    13381 aaattgagga atacaaaata aatcatccgc cctttaatat gtcaattgct agataaattt
    13441 ttagggtgtc ccattgctga aaacaggagt aaaaataaat aaagacctag actaattaat
    13501 ataaattacg tttcattacc cctggtaaat taaactataa caataacgat tttttttaaa
    13561 aaaaagtttt attacctttt atataatttt aaggtgagtc tatatctaac ataaaaaact
    13621 tattctctac taataaactt ttaatactca gatacatatt gtcccaatta tttttaatat
    13681 catcttgtgt aaaccattta accatttcta ttttttcttc tttttgtggt attagaggtt
    13741 gaccttcttt ggctttcata ctaaaccaat gagtctcttt taagtaagct tcattattag
    13801 aaaagtatat atgatatgtt ggttctaatc gttttactaa agtcaaatca caaacaccag
    13861 tttcttcaca tacttctcta atagcagctt tttcatcact ttcaccataa tctattttcc
    13921 cttttggtaa atcccatact ccattacgca agataaataa tattttatcc ttttcttcat
    13981 taaaaactac tccaccagcc cccaaaacaa ccttaaaaag gttataaaat tttatataaa
    14041 agtcttctaa tttataatta attaaaatca attgttgatg taaatcatca tcagcaaatt
    14101 gttttaataa atcaataatt tcattcaaat ctggttgata tttaataagt actttttcat
    14161 taatatgttt taaaatttga atattgtcag ttattaaaat tattttattt ttgatgaaaa
    14221 ttttatacat tatagttatg atttttaatg agcaaatttg tgaacagact tgtaaaattc
    14281 ttttgcaaat taaagcaata aaattaaatt atacaaatcc atttacatgg acctcaggta
    14341 tcaaatctcc catttattgt gataatagaa ttattttaag ttatccagat caccgcactg
    14401 agattgcaaa ttttttaaaa gaaatagtaa ttaaaaaatt ttcagatgct gattacatag
    14461 ctggagtagc tactgcaggc ataccaatgg ggaccttgat agcccatata ttaaataaac
    14521 catttattta tgtaaggcct aaagctaaag aacatgggct tcataatcaa atagagggtt
    14581 ttttgccaga aaataaaaaa gtagtagtag tagaagatct tatctctaca ggaaaaagta
    14641 gtaaaaatgc tattagtgct atacgtgagg ctaactgtga agtaataggc ttgatatcta
    14701 ttttttctta tgcattctca aaagtagaaa atgactttaa taataatgga attaaggttt
    14761 tttcgctagc cgattacccc aatctattac ctatagctat tgcaaataaa tatattccat
    14821 atgatgtttt acatatttta gaacaatggc atttagatcc taagaattgg tattcaaatt
    14881 tttttgaata ataattatat tgctaattta ttttagtgta gaaatattat ttttttttaa
    14941 taagacttag gtttttagtt attataaaat atttttttaa ctttgttttt taatttatta
    15001 aaattataaa aaaataaatt atggaaaaat cagaattagt tttgcaacct gatggtagtg
    15061 tatatcattt agcattattt cctcatgaga tagcagatac tattatatta gtaggtgatc
    15121 ccgaacgttc agattgggta tctagttttt ttgacaagat agaagtgagt agacgcaatc
    15181 gcgaaataat tagctataca ggttattaca aaaataaaag aatttctgta gtctctacgg
    15241 gcatgggaac agataatata gatatagtag taaatgaact cgatgcttta gcaaatatag
    15301 atttagaaaa aagagaagta aaaagagaac ataaaagttt aaatttgata cgcataggta
    15361 cctctggagc tctgcataat aaaatagata taaacaatgt ggttatatct gaaatggccc
    15421 ttggcatgga tggtttatta cactattaca agcatgatag ctctattaga gaaactgatt
    15481 tagaaaaagc agttttacaa aaagtaaatt ggccagatac acttcctaaa ccatacatag
    15541 ttagtggtga ccaaggactt tttaataaat taaattctga tcaatttcac aaaggtatta
    15601 cggtaacagc tagtggcttt tatgcaccac aaggtagagt cttacgttta ccattggcat
    15661 atcctgagtt gataaatcaa tttgaaaatt ttgaatacca agggcatata cttgctaact
    15721 tcgaaatgga aacatcagct ttatttggtt tagctaaact aatgggacat agggcatgta
    15781 ctctttgtgt agcgattgct aatcgagtaa caaaagattt tagtgaagat tactctcaag
    15841 ctatcaaaaa tttggcttta catgttttag ataaaatgac agaataaatg atatgtcaaa
    15901 ttaaaaagtt tttttaattt tgtaacaaag tatttaaaaa aaataaaaat atagagggat
    15961 aatgaaaagg gaagaagtat attcaaaagc cgtaaaagca ggtaggcgta cctatttttt
    16021 cgatgttaaa gaaaataact caggagatag atatattact atcacagaga gtagaaaaga
    16081 attagacgaa gtaacagggc aattcggtta taaaaaatct aaaatatttt tatataaaga
    16141 ggatatggat aagtttgctc gtggtttacg atatgctata gaattcattc agacaggtgt
    16201 agatcctgag gaaggtaaag aagagatatt aaattctcat aaatctgatg gacaaactga
    16261 ggattttaat aaattagagg aatctgataa cgaagatata attttctaaa aaaatgggca
    16321 aagtaatagc aattgcaaat caaaaaggtg gagtcggtaa aactactact gcattgaatc
    16381 taggcggagc gttcgcagca ctagatttaa aaacattgat cattgatgct gatccgcaag
    16441 ctaattctac atctggtctg ggaatagacc caaaagctgt tgataaaagt atttatgaat
    16501 gtattattag caatgaagcc cctgataaag ccatagtaga tacctctacg cctaatcttt
    16561 ttttattgcc agctcatata aatctagttg gagctgaaat agaacttata aatatggaga
    16621 atagagaaaa aaagatgaag acagtagtag atattatcaa aaatgaatat gaaatcattc
    16681 tcattgattg ttctccatct ctaggtatca tcactataaa tgcactaaca gcagcagact
    16741 cagtgattat cccagtacaa tgcgaatact ttgctctgga aggattaggg aaattattaa
    16801 atacaattag aatagtgcaa agtaggttaa atcctaactt agatatcgaa ggtattttat
    16861 tgactatgta tgatagacgt ttgcgtttag caaatcaagt ggtagacgaa gtgagacatc
    16921 actttaaaga tatcgtattc gatactataa ttcctcggaa tactcgtata ggcgaagcac
    16981 cttcttatgg agaaactatt attactttcg atactactgg tagaggagct caaagttata
    17041 ttgcactagc taaagaaatt ttgaataaat caaagaagga aaatgtaaat gtcagcaagt
    17101 aaaagacctg cattgggaag aggattagaa gctattttgg gtgatattga tagtgattat
    17161 ttcacttcac cagctttccc tttaatacct attgatcaga tagagagcaa tccgtatcaa
    17221 cccagaacag attttgatga agacaatctt tatgaattag cagaatctat aaaacattta
    17281 ggactaattc aacctattac agttagaaaa attgatgata aaaaatatca attgatctct
    17341 ggcgaaagac ggttgagagc tgctaaatta gctaatttaa ctgaaatacc cgcttttgtt
    17401 aaagatgctg atgacgatgc tttgctagaa atggctctag tagaaaatat acaacgtcaa
    17461 gatctcaatg ctatagaaat cgcaaactct tatcagaaat taattgaaga gtttgacctt
    17521 acccaagaag agctcagttc gcgtgtaggc aaaaatagag ccactatttc taattatttg
    17581 agattattaa atctgcctcc agctatacaa gcagccatta gatatgataa aatatctatg
    17641 ggacatgctc gcgcactggc atctatagaa gatgaaaaag atctaatgac aatttttaaa
    17701 aaaataattg atgaggaatt atctgttcgc aaagtagagc agttagtatc taatctgaaa
    17761 aataaaaaaa cagataacca tcctacacaa tgtcagattt ctcaaaaagc tgaaaatata
    17821 ataaagaata ttaaagaaaa atataatatc gaagctaagt caaagcttac tactaaaggc
    17881 aaagtagtgg tacaattaaa atttaataat gatgaagaaa tggaggattt ttttaatatt
    17941 tttttaaatg aataagtata tatttatctt tattatctct atttcactct gtataaatat
    18001 aaattcacaa aatcttatta ctggttatag tttcgattct tatagattaa attactctta
    18061 ttcgcaaaag aatgattcta tccatagtcc taaaaaagca gctctttatt catccttgtt
    18121 gcctggactt ggacagggat ataataaaaa attttggaaa atacctatta tttatgccgg
    18181 tctaggtgga tcagcatatg ctttttttta taatcgtaaa tattatgtag actatcgtga
    18241 tgccatagag atgaggctag atgatgaccc taacacgatt gatccttata ttgataagta
    18301 ttcgttagaa caattgtctt attataagga cttttatcag cgcaatatag aaataagtgc
    18361 tattgtattt gtatgtgttt acttactaaa tattttagat gctatagtag atgcacatct
    18421 atttgatttt agtataaata ctaaactatc ttatttacct gtttataata atacattcat
    18481 catccctact gtttcaataa attacaattt ttttaaaaaa aataaaaatg ataaaattag
    18541 ctataatagg atatggtaaa atgggcaaag aaattgaaaa ctgtgccaaa gatttagatt
    18601 ttagtattgc ctgtattatc gataaagaca ataatctgag aaatgacatc atacgatata
    18661 aacctaatgt ggctattgag tttactaatg ctgatgcagt cgtaaataat ataaaaactt
    18721 gtgttgacat gaatttgccc gttgtttcag gtactacagg ctgggacaaa aatattgatg
    18781 aagtaaaaaa ttatactatt cgcaataatg gaaatgtctt gtggggtagc aattttaata
    18841 ttggagtaaa cctctggttt gattttttaa atgaattggc ttgtattatg aaaaattatg
    18901 acctttataa agtagaatta gaagaaatac atcatattca taaattagat aaacctagtg
    18961 gtacagctat tcaagcagct aatatattga tgaaacaata cccaatttta aaaaattgga
    19021 gcctagacca tactgaggat acattacata taaattctat cagacaaggc gaagaagtag
    19081 gtactcatag agtaattttc aaaagtccct acgatcagat tagttttgaa cataaaagtt
    19141 tttcgcgtaa agctcttgcc gtaggggcct tagctggggc taagtggata attgataatg
    19201 ctggcttttt tatctttcat gatgtatatc aaagtgtttt cgaaaagttt aacaaagctt
    19261 gaatttttaa taaaataaaa actaacatat gcttagcaat aaatattaaa atattatgag
    19321 caataatata gttcggcata tatttattat atcataattt atattaaaat tattaattta
    19381 tttattataa attttctaat tatttataat tagtaatttt gaatttataa taaaactaaa
    19441 taaaactaaa tataaaacgt ttatatgttt ttgaaaagaa tgaagtattt ttttataata
    19501 atattttttt taatagcaac ctatggcttt gctcagaacg aatacaatag attaatagaa
    19561 tttgcaggca tagtagtaac tgcagatagt ctacgtccaa taccatttgt acatatattt
    19621 gtaaaagaca gcaggagggg tactgtgagt gatattaatg gattatttag cattgtcggc
    19681 gaaaaagggc aaaccattgt ttttagtgct gtaggataca aaactgtatt ttttaagata
    19741 cctgatacat taaagcatac tttttataat cttatacaac caatgataac tgatactata
    19801 tacttagata aaacggttgt ttatccttgg ccatcaaaag agaagtttaa agaagctttt
    19861 attaatactg taccacctga tgatgattta gaaagagcta gaaaaaattt agcttttatg
    19921 gaatggaaag ataaagaatt taattatgta gctcgcgatg gacaatctgc ttataatcaa
    19981 atgataaaag agatatcata taaaaattat tatgcagggc aattgccacc aaataatcta
    20041 ttaaatcctt ttgcatgggc tgcatttgta aaagcttggc aagagggaca atttaaaaga
    20101 aattctcaac ctaataaatc acaagaatat tattacaatg aaccattaca ttaatatttt
    20161 atttttacta ttatttctta atttttctct ttattgtcaa gaaaatataa ttataaatgg
    20221 atatataaaa aatcaaaata atgagcctat tccttatgcc aatattggta tagtaggtac
    20281 taatctagga acagcttctc atcaaaatgg tttttttgaa atatctgtcc ccatcagtga
    20341 tacagtgata ataggaatta gtactttagg ttatgaaaga ttgtatgata cattgataaa
    20401 acctattaaa aatattacca aaacttatat actcaaaact acgagtatgt tattgccaca
    20461 agtagaaatc accgagcagg tagacatagc taaggggata caacgtttag atcctaaaaa
    20521 agctgaatta cttcctacgc cagggggaca agtagaaaca ttaataaagc ttatgggtat
    20581 gggagtacag tcaagcaatg aattaagcag tcaatattct gtgcgtggtg gtaattatga
    20641 tgaaaatcta gtgtatatta atgattttga gatatatagg ccactgttaa cacatagtag
    20701 cgaacaagaa ggattgagct ttttgaattc cgatcttatc agctctatcg atttctcatc
    20761 aggtggcttc gaagctaagt atggcgacaa gatgtcttct gtattagatg ctacatatat
    20821 tcgaccatct gatcgattga ctactataca ggctagctta ttatctgcta atatttctac
    20881 tatgggaact cttttaaaaa acaaaatgac ttatttggct ggttttagat ataaaagcaa
    20941 taaatactta ttgagtacat tacccactac tggagattat ttccctaaaa tttttgattt
    21001 tcaaacttat cttacttatc agtttaatga tagatctgaa ctagcagcac taggttattg
    21061 gaattttaac caatttactt tttatcctaa atctagagaa acttcgtttg gtactattag
    21121 tgaagcatat aaaataaata tgtatttcga aggtgttgaa aaaaatttat ataatatatg
    21181 gatgggtgga ttaaagtgga catatattac agactcaaat acaattttta aaactattat
    21241 tagcacttat tattctaatg agcaggagat atatgatgta ttgagtcaat attggttgca
    21301 tacccttgag atagatttcg aatctgaaac ttttggcagg caaggtgaat tgatgggaat
    21361 aggtagttat ttaaatcacg gtagaaatta tttggatttg caagtaataa atttagaggg
    21421 aaaaattaat cataagataa ccaagcataa tatagattta ggaattaaag gacaaggtaa
    21481 ttggtttgat tttaaaatgc gtgaatggat atatatggat tcggctggct attcattgcc
    21541 taacatacat gatagtatag gctatactca gccacaacct tatcaaatat taaatcttta
    21601 tgaatttcta cataacaaaa aaactatcaa cacttggcat ataacaggtt acatacaaga
    21661 tatgtggtca aagaagttta ttgacaaagg cactattact actattttag gatttagaac
    21721 tggatattac agtataaata atacttttat gtttagcccg agattttcta ttagttatac
    21781 gccagaatgg cagagattga tggcttttag actgtcatta ggtctatatc agcaacctcc
    21841 attttggaaa gaaatggtag gatgggatgg cactatttat gataatgtac ctaatcaaaa
    21901 atcttttcat tttgttttag gaatggagta ttcttttttg gcttggaata gaccgtttaa
    21961 atttatcact gaagcatatt ataaatatct ttataatctt gtaccttatg ttgtggataa
    22021 tattagaata agatatttac cagagcaacg ttctaaagct tatgctacag gtatagattt
    22081 gaaactatat ggagagtttg ttccagatgt agattcttgg attagtgtgt ctattatgaa
    22141 aaccgctgag gacattatag gtgattatta ttatatatat tataatagtg atggagaacg
    22201 tataataccc aattatattt caaataacat acctgtcgat agtgtaaaga tatcgcctgg
    22261 ttatattcct cgacctggcg atcagagaat aaatatttca cttttttttc aggattatgt
    22321 accaaataat ccgaatttta aaatgagctt ggctttacaa tttggtagcg gcttaccttt
    22381 cggaccgcct ggtaataaaa aatatttaca tataaataga acctccccat atcgtagagt
    22441 agacattggt ttttcggcta agctatttac aggagattct aaaatagcaa agacattgaa
    22501 agaatgctgg cttagcttag aagtattaaa tcttttgcaa attagaaatg ttgcatccta
    22561 tgtgtgggtg agagatgtta cagatagaat gtatgctgta ccaaattatt taacagatag
    22621 acagcttaat cttaaattaa tagtaaaatt ttaaaaataa taacgtaatt tatttattag
    22681 aagcgcaata aatgaataat tcttaaacta aagctgataa ataataattt ttatgagtat
    22741 ataagagaat tattattaa
//
LOCUS       DAOPRN010000030        22403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1926_length_22403_cov_93.679166, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000030 DAOPRN010000000
VERSION     DAOPRN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22403
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1926_length_22403_cov_93.679166"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PKI65_04830"
     CDS             <1..353
                     /locus_tag="PKI65_04830"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOB77798.1"
                     /translation="EIKFCQFGLDCFIINNPTDKDIIAAQNGLKEVSEAIKSNYYDMI
                     ILDEICIAIHYDLISIDEVVKVINLRNENTEIILTGRYAPQELIDIADLVTEMKEIKH
                     YYNSGIIARRGIEF"
     gene            616..1932
                     /locus_tag="PKI65_04835"
     CDS             616..1932
                     /locus_tag="PKI65_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOB77799.1"
                     /translation="MTQKKDEILYERAKKVLPGGASRNQVLRQPHPVYAASASGSYLT
                     DVNGKQYIDFSANIGSLIHGHAHPKIVEAVYEQMKKGTAYMVATEVEVEYAEYMIARI
                     PSFGKLRFLNSGTEAVMHLIKVSRAFTGKSKIAKCEGAYHGTYDVIEVSEKPNPSNWG
                     DKKRPNSVPHTPGVPKGVLDSTIVLPFNDLENTMNILEAHKNDLACVLIDPMPHRINM
                     EPASKEYVNTIYEWTRKNGSLLCFDEVITFRVNYSGLQEYYNVKPDLTSLGKIIGGGF
                     PIGAFGGRDDIMEVLDPIGKYYKYPLSGTFSANPISLIAGLTAMKLFDQDAVNNLNAL
                     TDKARSFIQEAINIAQVPVSMSGIGSIIRLHFSDKVPADYRENYFNETNQKEAIKKFI
                     DYLYDNGIICVNSLSFFFNTTMTDKEIEILADKVLSAFREVKPYFK"
     gene            1939..3141
                     /gene="pcaF"
                     /locus_tag="PKI65_04840"
     CDS             1939..3141
                     /gene="pcaF"
                     /locus_tag="PKI65_04840"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: protein motif:HMM:NF006551.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HOB77800.1"
                     /translation="MRTAYICDAIRTPVGRYGGALSSVRTDDLTALIIKAILARNPHV
                     DTNEIDEVVFGCSNQAGEDNRNIARMALLLAGIPYTVPGVTINRLCGSGMEAVAYASR
                     LIALGDADLIIAGGVESMSRAPYAMNKATKAFDRNVNIYDTTLGWRFPNPIMEKMYGL
                     DSMAETAENLAKEMNISREDQDFFAFMSQIKAKKAQNNGFLAEEIFPITIPQPKADPL
                     IFQKDEHPRDTSLEKLAALKPITRIDGTITAGNSSGINDGAASLLVASEVGINKYNLT
                     PLARIVGSQSAGVPPRIMGIGPVPATNKLFEKFNLSFDDIDLIELNEAFAAQSLACIR
                     SWNLQDDDPRINPQGGAIALGHPLGMSGARLVTTAVYQLKRNNLKRALTTMCIGVGQG
                     MAMVIEKV"
     gene            3335..3619
                     /locus_tag="PKI65_04845"
     CDS             3335..3619
                     /locus_tag="PKI65_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77801.1"
                     /translation="MDFEVLLSEIELKIRGIIQQNQELRNAVANLKKENEKLGKENIE
                     LKNELMNKDKEITNIKIAKIIDEKGITDQATRYFKKMINRIDNCIAMLKE"
     gene            3623..3913
                     /locus_tag="PKI65_04850"
     CDS             3623..3913
                     /locus_tag="PKI65_04850"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOB77802.1"
                     /translation="MKSEMININVSLGGLAIPLKVNVSDEAHIRKIVKALDEQVTIYA
                     RKYPGQNLQTLLSMLLIEQAVALAKLQQEHQFIEEDLGVRLEQLKTLLETAR"
     gene            4109..5638
                     /gene="rny"
                     /locus_tag="PKI65_04855"
     CDS             4109..5638
                     /gene="rny"
                     /locus_tag="PKI65_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005848820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOB77803.1"
                     /translation="MILVIEIAVIAIGIATWYFIIKRNAIRKRDSIIAEAQKQAEVLK
                     QEKIIEAKEKSLQYKTRAEEEIQQKVKKLDALENKLLQQEMMIKQKNEELQKKIKEYN
                     QHKEQLQKDQSVLTEKLNKTDVLIKEYQKQLERIAGLTIEQAREELVESLKEDARMQV
                     NNYVKNLVDEARMHAEDQSRKIVLETIQRTATEYSSEAAVSTFPISGDDMKGRIIGRD
                     GRNIKTLEAATGVEIIIDDTPDLIVLSSFDPVRREIAKLSLEILVADGRIQPARIEEV
                     VAKVKKQIEQEIIKVGKETLIDLGITNMHPELIRLIGKMKYRTSYGQNLLSHSKEVAL
                     MSATMAAELGLNPKLAKRAGLLHDIGKVPDNEPELPHAILGMKLAEKYKEHPEVINAI
                     GAHHDEVEPTTLLAPIVQICDSISGARPGARRDVIDAYITRIKELETIAESYNGVQKT
                     FAIQAGRELRVIVSADQLSDTEAFQLSMELSKKIEDSLTYPGQIKVTVIRETRAVSFA
                     K"
     gene            5670..6770
                     /locus_tag="PKI65_04860"
     CDS             5670..6770
                     /locus_tag="PKI65_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOB77804.1"
                     /translation="MLSDKFTISHEQLHSAIVEIFTSIGMPYDHAKLVADVLVEAELR
                     GIPSHGVVRIKDYLGLWSQNRLNINAKPKIEYETLSTALVDGDNGLGMISGNFAMQSA
                     ISKANDAGTGWVAVNNSNHFGIAGYYALMAIDHDMIGIAMTNANPTVAPTFSKQAMLG
                     TNPIAFAVPTKNEQVFLADFATAPIARGKIELLEKQGKSVPKGMLQTCDGIPVTDPTA
                     IKHGGTILPLGGDYEHAGHKGYCLTAIVDILTAVISGANFGPFVPPQVSYIKEKNNCP
                     GKGLGHFFGAIRLDAFRPAEKVKEYMDLWIQTMRSALPIDTEQPVLIPNDPEIEMIDF
                     HKTNGITLLPEILNELKKTLNELSIPIPDNLT"
     gene            6826..7383
                     /locus_tag="PKI65_04865"
     CDS             6826..7383
                     /locus_tag="PKI65_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOB77805.1"
                     /translation="MEAKDKMTNEELNQEKEQDINNDIIEEKEVDLQKQFDELQEKHS
                     ELNDKYLRLCAEFDNYKKRTSKEKMNLIDTANMQLIIDLLPIIDDFERGLESIEKNQT
                     LDAIAEAYKIIYQKFMNILAKYGVEAINPIDEPFNMDLHEAISTIEGPPEKKNMIADV
                     VLKGYKLNLQDRPVILRYAKVIVYQ"
     gene            7408..8532
                     /gene="dnaJ"
                     /locus_tag="PKI65_04870"
     CDS             7408..8532
                     /gene="dnaJ"
                     /locus_tag="PKI65_04870"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOB77806.1"
                     /translation="MAKKDYYDILGVNKDASEDEIKKAYRKLAMQYHPDRNPNNKNAE
                     EKFKEAAEAYEILSDPEKKHRYDQFGEAGINGSGFSDIDDIFSHFSDIFDDFFGFGGR
                     RSGSYYQSYVKKGSNLRIRIKLSLEEILTGIEKKITIQKKVICHKCNGTGAKSKDSIT
                     TCPTCHGTGQVTHITSTLFGRIQSTNVCSSCNGKGKIISERCPVCNGTGIEDAKETIT
                     INIPAGALQDMQLTLTGQGNHVPDGIPGDLIVMIDEEEHPLFKRDGLNIFYEHYISYP
                     EAVMGTEIDIPILNGKATIKIPPGTQPGKLFRLKNKGLPDINSSKKGDFIVSINIHVP
                     DNVTKEEKNLLEEMKKMKHFNVQKQSSGKSLFERLKEYIQ"
     gene            complement(8535..9920)
                     /locus_tag="PKI65_04875"
     CDS             complement(8535..9920)
                     /locus_tag="PKI65_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOB77807.1"
                     /translation="MIFKRLIFLFLILIFNFNLSSQSLEPQKENITRILIIFDASNSM
                     YGHWQSDTKINIAKRLLNNILDSIADIPNLQLALRVFGDQRDFPPQDCNDTRLVVPFG
                     EGNAKKIAHFIKYLTPRGTTPIANTLAAAANDFTPCDNCRNIIILITDGIEECRGDPC
                     AVSQDLQKRGIVLKPFIIGIGQNFAKEFECVGDYYDGSTEEQFQKALKIVISYALNST
                     SCQVNLLDQLGNPTETNIPMTFYNRLNNQIMYQFVHTLNTKGVPDTLLLDPLINYRIQ
                     IHTIPPVYIDSLGLTPGKHVVASAYTPQGELFVKFASMSIEKSFVPIIVRKHNDKNTL
                     NVQYTNKKEKYLVGTYDLELLTLPRLYINDVEIKQSHTTTVEIPPPGNAIIYLPANGY
                     ASIFQRKKDKDLELVINLKTDENMQTIQLQPGNYTIVFRAKYVNKSFYTIEKKFTIES
                     NKNTTINLNFR"
     gene            complement(9910..10752)
                     /locus_tag="PKI65_04880"
     CDS             complement(9910..10752)
                     /locus_tag="PKI65_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOB77808.1"
                     /translation="MNDEKLQNIATQVRRDIIRMTNMAKSGHPGGSLGCTDFLTLMYF
                     EIMDIDPDHFTLEGPNEDIFILSNGHISPAFYSILARRGFFPIEELATFRRINSRLQG
                     HPTPSDHLPGVRFASGSLGQGLSIAIGCAIAKKLSNDTKIIYSLHGDGEVQEGQIWEA
                     ALFAGSKKIDNLISTIDYNGLQIDGPVDQVNSLGNLRAKWEAFGWYVLEMDGHDFSDM
                     RKTIKIAQNNLGKGYPIMILMHTIMGKGVPFMENDHKWHGTPPNDEQATEALKNLKST
                     LNDF"
     gene            complement(11070..11675)
                     /locus_tag="PKI65_04885"
     CDS             complement(11070..11675)
                     /locus_tag="PKI65_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOB77809.1"
                     /translation="MIMSPIQKILDGYRILLCSKSPRREQLLSKMGIDFIKVENNYKE
                     DIDTNLSPDELVINLSKQKSISYTLLKNKDILLTVDTIVWFDNQLIGKPKNRVEAIKI
                     LKTLSGQWHKVFTGITIRNNLLMTSFYDVTEVKFKPLRDEEIEYYVDNYEPYDKAGGY
                     GAQEWLGYIGIERIEGSFFNVMGLPTARLYDELINFLKKIK"
     gene            12055..14658
                     /locus_tag="PKI65_04890"
     CDS             12055..14658
                     /locus_tag="PKI65_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOB77810.1"
                     /translation="MEANYTIQIKNVLKRATEEAIKTGVPYIDDVHLFLGLMMEPNND
                     AIKIMKIFNVNPTNARLYFYDFIQSRKSQGQLFTFDNMLEDVNYLNEVPFESKAEQIL
                     KIAYLEAKALGAERADTEHLLLSFLKYNDNIVGEYLEEQGLNYNDVKQYIISHINSKT
                     DENASFTFSLEDDDDESEDDFYGGTSKRFSQTGKEQRSKTPVLDSFGRDLTQMAIENK
                     LDPIIGREKEMERVVQILSRRKKNNPVLIGEPGVGKSAIVEGLAIRIAQKKIPRPLYN
                     KRVVALDLAALVAGTKYRGQFEERVKALLNELEKNPDVILFIDELHTIVGAGSASGSM
                     DASNMFKPALARGEIQCIGATTLDEYRQYIEKDGALERRFQKIIVDPTDPEETIEILH
                     RIKDRYEDHHLVKYDDKAIEACVYLTDRYVTDRALPDKAIDVMDEAGARVHIKNINVP
                     EAVLKIEKSIEEAQQNKLKAISMQKYEIAAKFRDQEKELQKLLEEERIKWEKEASQHR
                     EIVTEDHIAEVVAMMTGIPVARIAQSESERLISMADDLKNIIIGQDEAIDTIVRAIRR
                     NRAGLKDPNKPIGTFFFLGPTGVGKTYTAKMLSKYLFDSEDALIRIDMSEYMEKFSVS
                     RLIGAPPGYVGYEEGGQLTEKIRRKPYSVVLLDEIEKAHPDVFNLLLQVFDEGHLTDS
                     MGRRVDFKNTLIIMTSNIGTRQLRDFGKGIGFATGTKTADDDYSRSVLENALKKAFSP
                     EFLNRIDEVVIFKNLQKEEIYKIIDIELKGLVDRLRNMKIYFEISDEAKDFIIERGWD
                     PAFGARPLKRAIQRYIEDPLAEEIIKKTLMENDKVTFSYKTGDNKLSLTIEHNKANLM
                     FDAPDSKVSDK"
     gene            15042..15962
                     /locus_tag="PKI65_04895"
     CDS             15042..15962
                     /locus_tag="PKI65_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HOB77811.1"
                     /translation="MAYFLIDNNTKLQLIKEKPFKLEKDIQKITELNLKEIFGLEFVC
                     SEFTVKNFRIDTLAYDRESNSFVIIEYKKDKNFSVIDQGFAYLSCMLNNKSDFILEYN
                     EHNKNSLKREDIDWSQSKIVFISSSFTIYQKEAINFNDLPIELWEVKRYCNNTISYQQ
                     IINKGSQESIRAISKLDSSISNITNEIKIYSETDHLAKASEQIIELYEMLKNKILTNY
                     DDINVKIRKYYIAFISKTNFVDIRISKKDIKIWLNLPKGKLNDPQNIARDVSAIGHWG
                     NGDYELILKNDDNIEYIMYLIKQAYEYNKK"
     gene            16079..16939
                     /locus_tag="PKI65_04900"
     CDS             16079..16939
                     /locus_tag="PKI65_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB77812.1"
                     /translation="MKNLLLLIILIYSFNILSGQSKRNLNFNEPISNINTKDAMIFWE
                     SFEGAEFPPTDWTQNITDSLFTWEKASSFPQDGQYFVHVLYDELDTSIWQNEWLITKS
                     INLIGYQSVILNFYFQMSKYWGIKPYDNYDLYVIAANDGVNFTDTIWSELSTDTSTWT
                     SWEWVNAQIDLTNYIGSNQLKLAFVYYGRDGAEASIDAISISTTGNLYDNNLDKLQIY
                     PNPTSDYLYIEAPYNTRVQIYDLLGNEIYNKLINDKLCKIDLHTYPCGIYILKLIRQN
                     ISKTFKIVKN"
     gene            17106..18779
                     /locus_tag="PKI65_04905"
     CDS             17106..18779
                     /locus_tag="PKI65_04905"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOB77813.1"
                     /translation="MKKNFLVLTLLLFASAYSSYACTNFIVTKGASTDGSVMITYSAD
                     SHTHYGDLYYSPAAVYPDGTMLDIYEWDTGKYLGKIKQAKKTYNVVGNINEYQVAIGE
                     TTFGGREELFTQSGAIMDYGSLMYIALQRSKSAREAIRVIAELVAEYGYASEGESFSI
                     ADKNEAWIMEMIGKGEGEKGAVWVAVRIPDGYVSGHANQARIRQFTLANGKTSITEKD
                     FNKKLYNPKVEVIYSSDVIEFARKKGWFNGKDEEFSFSDTYNPVTFGGARQCEIRVWS
                     MFRQITDGMDEYIDYVSGKNLSHRMPLYVKPNRKISIKDMMFFMRDHLEDTPFDMRND
                     IGAGPFKLPYRWRPLDWTIDGVTYTNERATATQQTAFSFITQSRNWLPDEIGGIIWFG
                     VDDAASCVYTPIYSASRKVPHSYAQGNGAIMTWSDDAAFWVFNQVANFAYTRYRIIHP
                     DIEQKQDSLETAFINKVKEIDDYALQLYEINPYQAIDYVSTFSADNADRLVMYWKEFY
                     KFLFLKYMDGNVKYVIPGERNPKVEWPGYGEEWYRRIIQETGDKFRVIE"
     gene            18813..19742
                     /gene="miaA"
                     /locus_tag="PKI65_04910"
     CDS             18813..19742
                     /gene="miaA"
                     /locus_tag="PKI65_04910"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOB77814.1"
                     /translation="MKDIPLLLVVLGATATGKTRLAVKIAIEINGEIISADSRQVYKG
                     LDLGTGKDLNEYKINTTDIPYHLIDIRSIDQTYSVYDFQRDALVAIEKIINKNKTPII
                     CGGTGLYIEALLLNYKFNKFNPDVELREKLSKMSIHELDLILKQLDSNIIVEKNNRHR
                     YIRAIEIALQNNNNSNEQDILEYNINKSLVFGIRYPRDMLRERIHKRLLDRIEAGMIE
                     EVKILLENNIDPNKLIAFGLEYKFITMYLLGKLTYDEMIDKLYTAICQFAKRQETWFR
                     RMEKRNININWIDGRLEEEKKIKEILNKINNEI"
     gene            complement(20161..21249)
                     /locus_tag="PKI65_04915"
     CDS             complement(20161..21249)
                     /locus_tag="PKI65_04915"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOB77815.1"
                     /translation="MKKLIVLLLMTLISIGVIDAQKKPSILFESRVYDFGQIKEEDGR
                     KTARFAFKNIGNDTLKILNVRASCGCTTSDYTRTPVLPGQSGYVDVAYNPLGRPGNFN
                     KIVTVITNDPDNPNVVLTIKGSVIPKPKTKADNYPQVLGNLRFKSNHITFNDIKDTEI
                     KTDTIYVYNPSDRKITIYSENLPSFLKIHIPAGGITIAPDQELKIPISYDAKARNDYG
                     YVFDNFRLKTDDIDQPVKQMYVSANIIQDFSWMTEKDKTNAPKIVFEKTEVVFDTIKM
                     GESVIVEFPFRNEGKNDLKILKVKSSCGCTAVAPEKNILKKGEKSSIKVTFNTAGRQG
                     NQQKSVTVITNDPEHSIVVLTLKGYVKN"
     gene            complement(21415..22236)
                     /locus_tag="PKI65_04920"
     CDS             complement(21415..22236)
                     /locus_tag="PKI65_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOB77816.1"
                     /translation="MSERVDWRGWITWILINAFMILELVKILNNNELWSSQAIILFCM
                     IIFSIVLGGFILIKYKKEIYALFLLGFAIISMIFGVLVWKWYIKEIATLFLVLGIFTG
                     MSLRMKANDIAKTMIDGAKDIASAALIVGLASAIIVILEDGKIMDTILYATANVIPEN
                     SKFLAVQGMYIFETVLNFILPSGSAKAALTMPIIAPLCDIIGLSRQLGVLAFQLGDGF
                     TNMITPVSGVLMGVLGMAKIQYNVWIKYIWKFILILFIIGALVHIPPIMFQLPGF"
BASE COUNT         8307 a         3093 c         3696 g         7307 t
ORIGIN      
        1 ccgaaataaa attttgccaa tttggactag attgttttat tattaataat ccaactgata
       61 aagacattat agcagctcaa aatggactaa aagaagtcag tgaggctatt aaaagtaact
      121 actatgacat gataatttta gatgaaatat gtatagctat ccattatgat ttaatctcca
      181 tagatgaggt agtgaaagta ataaacctaa gaaatgaaaa taccgaaatt attcttactg
      241 gacgttatgc gccacaggaa ttaatcgata tagcagattt agttactgag atgaaagaga
      301 ttaaacatta ttataatagt ggtattatag ctcgtagagg tatagaattt taaagatttt
      361 atataaagta ttgatattat agatactaat taatcatttt ggtatacaaa ataatgtttg
      421 ttaatattta aaatcattta acttagaaaa aaattattaa aaatttaatt aacccctaaa
      481 tcataaacaa aaatattttt aactcaaaaa catttggagt ttaaaaatta aattataaat
      541 ttgcaacata ttatttatta aaactgataa aaaataatat ttaattttgt accacaacaa
      601 aaaaatttta taaatatgac acaaaaaaaa gatgaaattt tatacgaaag agccaaaaaa
      661 gtattacctg gtggagctag tagaaatcaa gtattacgtc agccacatcc agtatatgca
      721 gcaagtgcat ccggctcata tttgacagat gtcaatggga agcaatatat agatttctca
      781 gctaacattg gatcattgat acatggtcat gcacatccta aaatcgttga agcagtatat
      841 gaacaaatga aaaagggaac agcttacatg gtagctacag aggtggaggt agaatatgca
      901 gaatatatga ttgcgcgcat cccatcattt ggtaaattaa gatttttaaa ttccggcacg
      961 gaggctgtaa tgcatctaat aaaagtatct cgtgctttta ccggtaaatc taaaatagct
     1021 aaatgcgaag gagcatatca tggtacttat gatgtaatag aagttagcga aaaacctaat
     1081 ccttccaatt ggggggataa gaaacgacct aattctgtgc cacatactcc tggggtccca
     1141 aaaggtgttt tagattcaac aattgtcctt ccttttaatg atttagaaaa cactatgaat
     1201 attttagaag ctcacaaaaa tgacctcgct tgtgtactta tagatcccat gcctcataga
     1261 attaatatgg agcctgctag taaggaatat gtaaacacta tatatgaatg gacccgcaaa
     1321 aatggaagct tattatgttt tgatgaagtg ataacattta gagtaaatta ttcaggttta
     1381 caagaatact ataatgtaaa accagattta acttctttgg ggaaaataat aggtggtgga
     1441 tttcctattg gagcttttgg tggtagagat gatattatgg aagtccttga tcctattggt
     1501 aaatattata aatatcctct ttctggtaca ttctcagcta atcccatatc tcttattgct
     1561 ggcttaactg caatgaaact ttttgatcag gatgcagtta ataacttaaa tgctttaact
     1621 gataaagcta gatcgtttat acaagaagct attaatatcg ctcaagtacc tgtatctatg
     1681 tctggtatag gttctattat tagattacat ttttcagata aagtgcccgc tgattataga
     1741 gaaaattatt tcaatgaaac aaatcaaaaa gaagcaataa aaaaatttat tgattattta
     1801 tacgataatg gcattatttg cgtcaactca ctaagtttct tttttaatac tactatgaca
     1861 gataaagaaa tagaaatatt agcagataaa gttttatctg cttttagaga agtaaaacca
     1921 tattttaaat aataatttat gagaacagct tatatttgtg atgctatacg tacccctgtc
     1981 ggaaggtacg gtggtgcttt gtctagtgtt agaaccgatg atcttacagc tttaattatt
     2041 aaagctatat tagctcgtaa tccgcatgta gacacaaatg aaatagatga ggtagtattt
     2101 ggttgctcta atcaagctgg tgaagataat agaaatatag ctcgcatggc tttgttatta
     2161 gcaggtatcc catatacagt gccaggggtc actattaatc ggctttgtgg ttcaggtatg
     2221 gaagcagttg cttatgcttc tagattgatt gctttgggtg atgcagatct catcatcgca
     2281 ggtggtgtag agagcatgag tagagctccg tatgctatga ataaagcaac aaaagctttt
     2341 gatcgcaatg taaacatcta tgatactact cttggctggc gtttccctaa tcctattatg
     2401 gaaaaaatgt atggattgga tagcatggca gaaactgctg aaaatctggc taaagaaatg
     2461 aatatttcac gcgaagatca agattttttt gcttttatga gtcaaataaa agcaaaaaaa
     2521 gcccaaaata atggtttttt ggctgaagaa atctttccaa ttactattcc acaacctaaa
     2581 gcagatccat tgattttcca aaaagacgag catccacgtg acacatcttt agaaaaatta
     2641 gctgctctta aacctataac tagaatagat ggtactatta ctgctggtaa ttcctctggt
     2701 attaatgatg gtgctgctag cttacttgta gcttctgaag ttggtattaa taaatataat
     2761 ctaacacctc ttgctcgaat agttggttca caatcagctg gtgtccctcc tagaattatg
     2821 ggcataggtc cagtgccagc tactaataaa ttgtttgaaa agtttaacct ttcttttgac
     2881 gatattgatt taattgagtt aaatgaagct tttgctgctc aatctttagc atgtataaga
     2941 agttggaatt tacaggatga tgatccacgt atcaatccac aaggcggtgc tatagccctt
     3001 gggcatccgt taggaatgtc aggagcaaga cttgttacta cagcagtata tcaattaaaa
     3061 agaaataacc ttaaaagggc tctgactact atgtgtatag gtgtaggaca aggtatggct
     3121 atggttatag aaaaagtata aataaaaccc aagcattcta taataaaaaa tcaatttaaa
     3181 agttttattt ataattattt atgacataaa gtttaaacat attttaacat ataagaaata
     3241 aaaaaaatta actttactaa acttataaat taaaaaaaaa caatgtaaaa aacttatatt
     3301 tatatcaaaa aaaaatatta attttgtata aaaaatggat tttgaagtat tactctcaga
     3361 aatagaatta aaaatcagag ggataataca acaaaatcag gagttgagga atgctgtggc
     3421 aaatttaaaa aaggaaaatg agaagcttgg aaaagaaaat atagaattaa aaaatgaatt
     3481 aatgaataag gataaagaaa taacaaatat aaaaatagcc aaaataatag atgaaaaagg
     3541 aataacagac caagctactc gctattttaa aaagatgata aatcgtattg ataattgtat
     3601 agctatgcta aaagaataag gtatgaagtc tgaaatgatt aacataaatg ttagcttagg
     3661 tggtttagca atacctttaa aagtaaatgt aagtgacgaa gctcatataa gaaaaatagt
     3721 taaagcctta gatgagcaag tgacaatata tgcacgcaaa tatcctgggc aaaatttaca
     3781 aactttatta tctatgttgc ttatagaaca ggcagtagca ttagctaaat tgcaacaaga
     3841 acatcaattt attgaagaag atttaggtgt tagattagag caattaaaaa ctttgctaga
     3901 aactgctaga tagctttata attacctgaa tttatttatc aattatttgc cacagtcatt
     3961 ttttaaaaaa aaacctcaaa attaaatgtt aagtagtttg taaaaaaaaa gttagcaaac
     4021 tacttgaaga tttagaaata aaaacgcaaa ttattgcgat tttttgttgt ataactaaac
     4081 taaaaacaat aaaactatgt caaatgtaat gattttggtg atagaaatag cagttattgc
     4141 tataggaatt gccacctggt attttataat taaacgcaat gctataagaa aacgtgatag
     4201 cataatagct gaagctcaaa aacaagcaga agtattaaaa caagagaaaa tcatagaggc
     4261 taaagaaaaa tctctgcaat ataaaactag agcagaagaa gaaattcaac aaaaggtaaa
     4321 aaaactggat gcacttgaaa acaaactatt gcaacaagaa atgatgataa agcaaaaaaa
     4381 tgaagagctg caaaagaaaa taaaagagta taatcagcat aaagaacaat tgcaaaagga
     4441 tcagtcagtt ctcactgaaa aattgaataa gactgatgta ctaataaaag agtatcaaaa
     4501 acaattagaa aggatagctg gattaactat agaacaagca cgtgaagaac ttgtagaatc
     4561 cctgaaagag gatgctcgaa tgcaagtaaa taattatgtt aaaaatttag ttgatgaagc
     4621 tcgtatgcat gccgaagatc aatctagaaa aattgtattg gagactatac aacgtactgc
     4681 tacggaatat tcttctgaag ctgcagttag tacatttccc atttctggtg atgatatgaa
     4741 aggacgcatt ataggtagag atggtagaaa tattaaaacc ttagaagccg ctacaggagt
     4801 agaaattatt atagatgata cgccagatct catcgtatta tcttcttttg accctgttag
     4861 acgtgaaatt gccaaattat ctctcgaaat tttagtcgct gatggtcgta ttcaaccagc
     4921 tcgcatagag gaagtagtag ctaaggttaa aaaacaaatc gagcaagaaa ttataaaagt
     4981 aggtaaagaa actctaatag atctcggcat tactaatatg catcctgaac ttattcgttt
     5041 aattggcaaa atgaaatatc gcacttctta tggtcaaaac ttgctctctc atagcaaaga
     5101 agttgctctc atgtcagcta ctatggctgc tgaattagga ctcaatccta aattagctaa
     5161 aagagctggt ctattacatg acattggtaa agtgccagat aatgaaccag agttacctca
     5221 cgcaatttta ggaatgaaat tagctgaaaa gtataaagaa catccagagg tcataaatgc
     5281 tataggcgcc catcacgacg aagtagaacc tacgacatta cttgctccaa tagtacaaat
     5341 atgtgattct attagtggtg ctagacctgg agctcgtaga gatgtaatag atgcttatat
     5401 tactcgtatc aaagaattag aaactattgc tgaaagttat aacggggtac aaaaaacttt
     5461 tgctatccaa gcaggtagag aattgcgtgt tatagtatct gctgatcaac tgtcagacac
     5521 tgaagcattc caattatcta tggagcttag caaaaaaatt gaagactctt taacttatcc
     5581 tggccaaata aaggttactg taattaggga aacacgtgca gtttcttttg ctaaataagt
     5641 ttatttaaaa aaatattttt aataatatta tgttatcaga taagtttaca atttctcatg
     5701 agcaattaca tagtgcaata gttgagattt tcacttcgat aggtatgcct tatgatcatg
     5761 ctaagctagt agcagatgta ctcgtagaag ctgagcttcg tggtatcccc tctcatggag
     5821 ttgttaggat aaaagactac ttaggacttt ggagccaaaa tagactcaac attaatgcta
     5881 aacctaaaat agagtatgaa actctatcga cggctctagt agatggtgat aatggactag
     5941 gaatgattag tggtaatttt gctatgcaat cagcaatatc aaaagctaat gatgctggta
     6001 caggatgggt agctgtaaac aattcaaatc attttggtat tgcaggttat tatgcactaa
     6061 tggcaataga tcatgatatg ataggaatag ctatgactaa tgctaatcct actgtagcac
     6121 ctactttctc taaacaagct atgcttggta ctaatccaat agcttttgca gtacctacta
     6181 aaaatgaaca agttttttta gctgatttcg ctacggcacc gattgctcgt ggcaaaattg
     6241 aattactcga aaaacaaggt aagtctgtac ctaaaggtat gctacaaact tgtgatggca
     6301 tacctgtcac tgatcctact gctataaaac atggaggcac tattttgcca ttgggaggag
     6361 actatgaaca tgctggtcat aaaggttatt gtttaactgc tattgtagat atcttgacag
     6421 ctgtaatatc tggtgctaat tttggacctt ttgtccctcc acaagtgagt tatattaaag
     6481 aaaaaaataa ttgcccaggt aaagggttgg gacatttttt tggtgctata cgactcgatg
     6541 cctttagacc agctgaaaag gtaaaggaat atatggattt atggattcag actatgcgat
     6601 ctgctctgcc tatagatact gagcaaccag ttttaatacc taatgatcct gaaattgaaa
     6661 tgattgattt tcataaaaca aatggtataa cgctattgcc cgaaatttta aatgaattaa
     6721 aaaaaacact taatgaattg agtataccaa tacctgacaa tttgacttga ttttgataaa
     6781 aggcaaacat tttgccagta aaaaaataaa aaaattataa tttttatgga agcaaaagat
     6841 aaaatgacta acgaagaatt aaaccaagaa aaagagcaag acattaataa tgatattatt
     6901 gaagaaaaag aagttgacct acaaaaacaa tttgatgaat tacaagaaaa acatagcgaa
     6961 ttaaatgata aatatttgcg actatgtgct gaatttgaca attataaaaa aagaacaagt
     7021 aaagaaaaaa tgaatcttat agatactgct aacatgcagc taataataga tctattgcca
     7081 atcatagatg atttcgaaag aggcttagag tctatagaaa aaaatcaaac cttagatgct
     7141 atagcagaag catataaaat aatttatcag aaatttatga atatattagc aaaatatggt
     7201 gtagaagcaa taaatcctat tgatgagccg tttaatatgg atttacatga agctattagt
     7261 actatagagg ggcctcctga aaagaaaaat atgatagcag acgttgtttt gaaaggatat
     7321 aaattaaatt tacaggatcg accagtaata ttacgttatg caaaagttat agtttatcaa
     7381 taaaataaat aaaaaaataa ttaaatcgtg gctaaaaaag attattatga tatactaggg
     7441 gtcaataaag atgctagtga agatgagata aaaaaggctt atcgtaaact agctatgcaa
     7501 tatcatccgg atagaaatcc caataataaa aatgctgaag aaaaattcaa agaagctgcg
     7561 gaagcatacg aaatattgag tgatcccgaa aaaaaacata gatatgacca atttggtgaa
     7621 gcaggtatca atggcagtgg ttttagtgat atagatgata tattttcaca ttttagtgat
     7681 atttttgatg atttctttgg ctttggcggg agacgtagtg gctcttatta tcaatcatat
     7741 gttaaaaaag gaagtaattt acgaatacgc atcaaacttt ctttagaaga aatacttaca
     7801 ggtattgaga aaaaaattac tattcaaaaa aaagttattt gtcataaatg taatggtaca
     7861 ggtgctaaat ctaaagacag catcactact tgccccacat gccatggcac gggacaggta
     7921 acccatatca cttcaacact ttttggtaga atacaaagca caaatgtatg tagctcctgt
     7981 aacggtaaag gaaagataat atctgaaaga tgccctgttt gtaacggtac aggcatagaa
     8041 gatgctaagg aaactattac tattaatatt cctgcaggcg ctttgcaaga catgcaatta
     8101 actcttactg ggcaaggaaa tcatgtacca gatggtattc caggagatct aatagttatg
     8161 atcgatgaag aggaacatcc gttatttaaa agagatggac taaacatctt ttacgaacat
     8221 tatatctcgt atcccgaagc tgttatggga actgaaatag atattccaat acttaatggt
     8281 aaagctacta ttaaaatacc accaggtacc caacctggta aattatttag attaaaaaat
     8341 aaaggcttac ctgatattaa ttcatctaaa aaaggtgatt ttatagttag tatcaatatt
     8401 catgtacctg ataatgtaac taaagaagaa aaaaatttat tagaagaaat gaaaaagatg
     8461 aaacacttta atgtgcaaaa gcaatcttct ggcaaatcac tattcgaacg cttaaaagaa
     8521 tatattcaat aatattatct gaaatttaga ttgatagtag tatttttatt actttcaata
     8581 gtgaattttt tttctattgt gtaaaagctt ttatttacat atttagctct aaatacaata
     8641 gtataatttc ctggttgtag ctgtatggtt tgcatatttt catcagtttt tagatttatt
     8701 actaattcta aatctttatc tttttttctt tgaaaaatac tagcatatcc attagctggt
     8761 aaataaataa tggcattacc tggtggtggt atctctacag tagtagtatg actctgctta
     8821 atctctacat catttatata taatctaggc aaagtcaata actcgaggtc gtaagtacct
     8881 actaaatatt tttctttttt atttgtatat tgtacattta gagtattttt atcattatgt
     8941 ttacgtacta ttattggaac aaatgatttt tcgatactca tagaagcaaa ttttacaaat
     9001 aattcgccct gaggtgtata tgctgatgca acaacatgct tgccaggagt gagcccaagt
     9061 gaatctatat aaactggtgg tatagtgtgt atctgtatcc tataatttat aagaggatct
     9121 aaaagaagag tgtctggtac accttttgta tttaatgtat gaacaaattg atacattatt
     9181 tgattattta atctattata aaatgtcatt ggtatattag tttcagtagg attacctaat
     9241 tgatccagta gatttacttg acaagatgtg gaatttagag cgtatgatat aactattttt
     9301 agtgcttttt gaaattgctc ttcagtacta ccatcataat aatctcctac acattcaaat
     9361 tctttagcaa aattttgtcc tatgccaata ataaatggtt tgagcactat accacgtttt
     9421 tgtaaatctt gagatacagc acacggatct ccgcgacact cttctatacc atctgttatc
     9481 aatattatta tattcctaca attatcacaa ggagtaaaat catttgcagc agcagcaagt
     9541 gtattggcta taggtgtagt accacgagga gttaaatatt taataaaatg agctattttt
     9601 tttgcattac cttcgccaaa tggcactact aatctggtat cgttacaatc ttgtggtgga
     9661 aaatctcgct gatcgccaaa aactcgaaga gctaattgta aatttggtat atctgcaata
     9721 ctatctagaa tattatttag taatcgcttc gcaatattta tcttagtatc actctgccaa
     9781 tgtccataca tactattgct agcatcgaat attatcaaaa tgcgagtaat attttctttt
     9841 tgcggctcca aagattgact agataaatta aaattaaaga ttaaaattaa aaataaaaaa
     9901 attaatcttt taaaaatcat ttaatgttga ttttaaattt tttagagctt cagttgcttg
     9961 ctcatcattt ggtggagtac catgccattt atgatcattt tccataaaag gtacaccttt
    10021 gcccattata gtatgcatca aaatcattat tggatatcct tttcccaaat tattttgagc
    10081 tatcttgata gtttttcgca tatccgaaaa atcatgtcca tccatctcta aaacatacca
    10141 accaaaggcc tcccatttgg ctcgtaaatt tcctaatgaa tttacttgat ctaccggtcc
    10201 atctatctgt aaaccattgt aatctatagt agaaatcaga ttgtcgattt ttttagatcc
    10261 agcaaaaagt gctgcttccc aaatttgtcc ttcttgcact tcaccatctc catgtagtga
    10321 ataaataatt ttagtatcat tacttaattt ttttgcaata gcacaaccta tagctataga
    10381 caatccttgt cctagagagc cagatgcaaa ccttactcct ggtaaatgat ctgacggtgt
    10441 gggatgtcct tgtaatctac tattaatgcg tctaaatgta gctaactcct ctataggaaa
    10501 aaaacctcta cgtgccaaga tagaataaaa agctggacta atatgaccat tagataaaat
    10561 gaaaatatct tcatttggac cttctaatgt aaaatgatca ggatctatgt ccatgatttc
    10621 aaaatacatt aatgttaaga aatctgtaca acccaatgaa ccgccaggat gacctgattt
    10681 agccatattt gtcatcctga ttatatcacg acgtacttga gtggctatgt tttgtaattt
    10741 ttcatcgttc attaaaatag ttttttacaa atatattaaa aattttttat taaaaaatat
    10801 gaaaaccata tttaaagatc ataaattaga aaattaatta gcaatgggca agaaggaaaa
    10861 cctagaattt attttttaat ttgtagtata ctacaaatat actacaaaat gtaactaatc
    10921 taaatagaac aaataaaata taatatcaaa caccaatgta atatgtgaaa aagagaggga
    10981 agtaaataaa aacaataaaa agaaaatgta aagtattgta gttttggaag tttatagatt
    11041 attagttttt aaagaagaat atttataatt catttaattt tttttaaaaa atttattaat
    11101 tcatcatata atcgtgcagt aggcaagccc ataacattaa agaatgaccc ctctattctt
    11161 tctattccta tataacccaa ccattcttgc gctccatagc cacctgcttt atcatatggt
    11221 tcataattat ctacgtaata ttctatttcc tcatctctaa gaggtttgaa ttttacttct
    11281 gtaacatcat aaaaagatgt cattaataga ttatttctaa tagtaatgcc agtaaaaact
    11341 ttatgccatt gccctgataa agtttttaaa atttttatag cctcgactct gtttttaggt
    11401 tttccaatta attgattatc aaaccataca atagtatcta ctgttaataa aatatcttta
    11461 ttttttaata atgtgtatga aatgcttttt tgtttactta agtttattac taattcatct
    11521 ggagataaat tagtatcgat atcttcttta taattatttt ctacttttat aaaatcaatt
    11581 cccattttac ttagtaattg ctctctacgt ggacttttag aacataataa tattctgtaa
    11641 ccatctaaaa ttttttgtat cggcgacatt atcatatcaa attaattact ttttacaaac
    11701 gtaagcattt ttttaaaaaa aatataaggg attataatta aattttaatt gtattaaatt
    11761 acattaaaag taaaatatat atgttttttt ttaaaaaatt ctaaattatt caaaggggtc
    11821 gacgtgtgtt ttactaagtt atatgtcaaa gtcttattat gagttataaa tattatatca
    11881 atgcttgaaa tataaaattg tacatatgat taatattcgc aagctgtcaa aataacatac
    11941 aaatattaaa aatattaggt ataattgaat tggtattttt tttgaagaac attggtatat
    12001 attaaaaaat atattatttt tgtataaaat tcaaaaaata ggagaaaaaa aataatggaa
    12061 gcaaattaca caattcaaat aaagaatgtg ttaaaaagag ctactgagga agccatcaaa
    12121 actggtgtac cttatataga tgatgtgcat ttatttttag gtttaatgat ggagcctaat
    12181 aatgatgcta ttaaaataat gaaaattttt aatgtaaatc ctacaaatgc aaggttatat
    12241 ttttatgatt ttattcaatc acgcaagagc caaggtcagc tatttacttt tgataacatg
    12301 ttagaggatg ttaattattt gaatgaagtt cctttcgaat caaaagcaga acaaatatta
    12361 aaaatagctt atttagaagc taaagcttta ggtgctgaga gagcagatac agagcactta
    12421 ttattatctt ttttaaaata taatgataat attgtaggtg aatatttaga agagcaaggt
    12481 ttaaattata atgacgttaa gcaatacatt atctcacaca taaattcaaa aactgacgaa
    12541 aatgcaagtt ttacatttag cctagaagat gatgacgatg agagtgaaga tgatttttat
    12601 ggtggtactt ctaagcgttt ctctcaaact ggaaaagagc aaaggtctaa aactccagtt
    12661 ttagatagct ttggtagaga tctcacgcaa atggctatag aaaataaatt ggaccccatc
    12721 atagggcgcg aaaaagagat ggagcgtgta gtccaaattc ttagccgaag aaaaaagaat
    12781 aacccggtat taataggcga gcctggtgtt ggcaaaagtg ctatagtaga ggggttagct
    12841 attcgtatag ctcagaaaaa aatccctcgt ccattataca ataaacgtgt agtggcctta
    12901 gatctggcag ctctagtagc tggtactaaa tatcgcggac aattcgaaga aagggttaaa
    12961 gctttattaa atgaacttga aaaaaatcct gatgttattt tatttattga tgaattacat
    13021 actatcgttg gtgctggtag tgcatctgga tctatggacg cttctaatat gtttaaacct
    13081 gctctagcac gtggcgaaat acaatgtatc ggggctacca ctttagatga atatagacaa
    13141 tacatagaaa aagatggtgc gctcgaacgt agatttcaaa aaatcatagt agatcctact
    13201 gatccagaag agactattga aatactacat cgaattaaag atagatatga agatcatcat
    13261 ttagtaaaat atgatgataa agccatagaa gcttgtgtgt atttgactga tagatacgta
    13321 acagatagag ctctaccaga taaagctatc gatgtaatgg atgaggctgg tgcccgtgta
    13381 catataaaaa acattaatgt tcctgaagct gtgttaaaaa tagaaaaaag cattgaagag
    13441 gctcaacaaa ataaattaaa agccatttct atgcagaaat atgagatagc tgctaaattt
    13501 agagatcaag aaaaggaact tcaaaaactt ttagaagaag aacgtataaa atgggaaaaa
    13561 gaagcttctc agcataggga gatagtaaca gaagatcata tagctgaagt agtagctatg
    13621 atgacaggta tacctgtagc tcgtatagca cagagcgaaa gtgaacgctt gatatctatg
    13681 gctgatgatt taaaaaatat aataatagga caggatgaag ccatagatac aatagtgaga
    13741 gctattagaa gaaatcgagc aggactaaaa gatcctaata aacctatcgg tactttcttt
    13801 tttcttggac ctactggtgt tggtaaaact tatactgcta aaatgctttc taaatattta
    13861 ttcgattccg aagatgccct aatcaggata gatatgagtg aatatatgga aaaatttagt
    13921 gtttcacgat taataggagc acctcctggc tacgtaggtt atgaagaagg ggggcaattg
    13981 actgaaaaaa ttcgtagaaa accatatagt gttgtattat tagatgaaat cgaaaaagct
    14041 catcctgatg tttttaatct tttactacaa gtctttgatg aaggtcattt gactgatagc
    14101 atgggtagaa gggtagattt caaaaatact cttatcatca tgacttctaa tattggtaca
    14161 cgacaattga gagattttgg taaaggaatt ggattcgcta ctggtactaa aactgctgat
    14221 gacgattatt cgcgtagtgt actggaaaat gctcttaaaa aagctttttc gcctgaattt
    14281 ctcaatagaa tagatgaggt agtgattttc aaaaatctac aaaaagaaga aatttataaa
    14341 attattgata ttgaactcaa gggtttagta gatagactta gaaatatgaa aatctatttt
    14401 gaaatatccg atgaagctaa agattttatc atagaacgtg gatgggaccc cgcctttggt
    14461 gctagacctc tgaagcgtgc tatacagaga tatatagaag acccattagc tgaagaaatt
    14521 attaagaaaa cattaatgga aaatgataaa gtcacttttt cttataaaac tggtgataat
    14581 aaattaagcc ttactatcga acacaataaa gcaaatctaa tgtttgatgc tcctgatagt
    14641 aaagtgtcag ataaataatt aatgatttca gatatattgt ttcaaaatat tacatgtttt
    14701 agatagtaaa tctttataat gaaccagaaa acaaagaact tttgttaata actaaataat
    14761 atcaaattct ttattatctc aaaactttga ttgaaagtaa tcaaagttta aatctatatt
    14821 gtatagagtt atcttttatt aattaagagg taaataaaaa aggggctcat atgagctcct
    14881 ttaattatgt aaaaaatagt ggtgttatta actaatacat cgactttgta ttaaaattaa
    14941 taagtgcaat attagtaata aattttttta acaaccatat ttttttataa atatttatta
    15001 agtgccttat taaaaaatca ttaaatttac aaaaaaaaat tatggcatat tttttaattg
    15061 acaataatac aaaacttcaa ttaatcaaag aaaaaccttt taaactagaa aaagatatac
    15121 aaaaaatcac tgaattaaat ttaaaagaaa tttttggttt agaatttgtg tgttctgaat
    15181 ttacagtaaa aaatttcaga atagacactt tagcttatga tagagaatct aactcctttg
    15241 taataattga gtataaaaaa gataaaaatt ttagtgttat tgatcaaggt tttgcttact
    15301 tatcttgtat gctaaataat aaatctgatt ttattttaga atataacgaa cataataaaa
    15361 attctttaaa acgtgaggat atcgattggt ctcaatcaaa gattgtattc atttcctcta
    15421 gctttactat ttatcaaaaa gaagctatta attttaatga tttgcctata gaattatggg
    15481 aggttaaaag atattgtaat aacacaataa gttatcagca gataattaat aaaggttcac
    15541 aagaaagtat tagagcaatt tcaaaattag atagttcgat ttctaatatt actaacgaaa
    15601 taaaaatcta ttccgaaacg gatcatctag cgaaagcttc tgaacagata atagaactat
    15661 atgaaatgct aaaaaataaa attttaacaa attatgatga tattaacgta aaaatcagaa
    15721 agtattatat tgcttttatt tctaaaacta attttgtaga tattcgtatc agtaaaaaag
    15781 atataaaaat ttggttaaat ctaccaaaag gcaaactaaa tgacccacaa aatattgctc
    15841 gtgatgtctc tgcaatagga cattggggta atggagatta tgaattaatt ttaaaaaatg
    15901 atgataatat tgaatatatt atgtatttaa tcaaacaagc atatgaatac aacaaaaagt
    15961 gaagcttttt atttgctgtg caaaggatga tcgctatgag agtaatttta aaaagaatat
    16021 taaggtatta ctttctaaaa atattattta tctttgccat aattaaatta attattttat
    16081 gaaaaatcta ttgctattaa taattttaat ttattctttt aatatactat ctggtcaatc
    16141 taaaagaaat cttaatttca atgaaccgat tagtaatatt aatactaaag atgccatgat
    16201 cttttgggaa agcttcgaag gtgcagaatt tcctcctact gattggactc aaaacatcac
    16261 tgatagttta tttacatggg aaaaagctag tagttttcct caagatggtc aatattttgt
    16321 gcatgtgcta tatgatgaat tggatacgtc tatctggcag aatgaatggc tcataactaa
    16381 gtctattaac ctaataggtt atcagtctgt tattttgaat ttttattttc aaatgtctaa
    16441 atactggggt atcaaacctt atgataatta tgatttatac gtaatagctg ctaatgatgg
    16501 ggtgaatttc actgacacta tttggagtga attaagcact gatacatcta cttggactag
    16561 ttgggaatgg gtaaacgctc agatagattt gactaattat attggtagta atcaactaaa
    16621 attagcattt gtatattatg gtcgtgatgg agccgaagct tctatagatg ctatatctat
    16681 ctctactacg ggtaatttgt acgataacaa tttagataaa ttgcagattt atcctaatcc
    16741 tactagcgat tatctatata tagaagctcc ttataatact agagtacaaa tttatgattt
    16801 attaggcaat gaaatctata ataaattaat taatgataaa ttatgcaaaa tagatctaca
    16861 tacctatcca tgtggtattt atattttaaa attaattaga caaaatataa gcaaaacttt
    16921 taaaattgtt aaaaattaaa atatctgaac cgcagattcc cagatgatta ttctgattaa
    16981 tcataggttt atttccaaat cataacaaat cacataaatc gtgataaaat tacgtcgcag
    17041 gcgatattta aaagggaaat tgtcaattaa aattattctt gtatatttgc aaaaattaat
    17101 aaattatgaa gaaaaatttt ttagtattaa ccttattatt gtttgcgagt gcatatagtt
    17161 catatgcatg tactaatttc attgtcacca aaggtgctag tactgatggc tcagtaatga
    17221 taacgtattc agctgatagt cacactcatt atggtgattt gtattattca cctgccgcag
    17281 tatatcctga tggaaccatg ctagatattt atgaatggga tacaggcaaa tatttaggca
    17341 aaattaaaca agctaaaaaa acttacaatg ttgtaggtaa tattaatgaa tatcaggtag
    17401 ctattggaga aactactttt ggtgggagag aagaattatt tactcagagc ggtgctatta
    17461 tggattatgg tagtttgatg tatattgctc tacagcgctc caagagtgct cgagaggcta
    17521 tacgagtgat agctgaacta gtagctgaat atggctatgc tagtgaaggg gaatcattct
    17581 ccatagcaga taaaaatgaa gcttggatta tggaaatgat aggcaaaggc gaaggcgaga
    17641 aaggtgctgt gtgggtggca gtacgcattc cagatggtta tgtcagtgga catgctaatc
    17701 aagctcgcat tagacaattc actctagcta atggtaaaac ttctattact gaaaaagatt
    17761 ttaataaaaa attatataac cctaaagtag aagttattta ttcatctgat gttatcgaat
    17821 ttgctagaaa aaaaggttgg tttaatggca aagatgaaga gtttagcttt tctgatacat
    17881 ataatccagt aaccttcggt ggcgctagac aatgtgagat acgtgtttgg agtatgttta
    17941 gacaaataac agatggtatg gatgaataca tagattatgt aagtggtaaa aatttatcgc
    18001 atcgcatgcc gctatatgta aagccaaata gaaaaatttc tatcaaagat atgatgtttt
    18061 ttatgcgtga tcatttagag gatactcctt ttgatatgcg taatgatatt ggtgcaggtc
    18121 catttaagtt gccttaccgc tggagaccat tagactggac tattgacgga gtgacctata
    18181 ctaatgaacg agctactgct acacaacaaa cagcttttag ctttatcact caaagtcgca
    18241 actggcttcc agatgagatc ggtggtatta tatggtttgg cgtagatgat gctgctagtt
    18301 gtgtttatac tcctatctat tctgctagta gaaaggtgcc tcattcttat gctcaaggta
    18361 atggtgccat aatgacctgg agcgatgatg cagcattttg ggtctttaat caagtggcta
    18421 attttgctta tactcgctac cgcattatac atcctgatat tgagcaaaaa caagattccc
    18481 tggaaactgc ttttattaat aaagtaaaag aaatagatga ttatgctctg cagttatatg
    18541 agataaatcc ttatcaagct attgattatg tttctacttt ctcagcagat aatgcagata
    18601 gattagtgat gtattggaaa gaattttata agtttttatt tctcaaatat atggatggta
    18661 atgtgaaata tgttattcct ggtgagcgaa atcctaaagt tgaatggcca ggttatggtg
    18721 aggaatggta tagacgtatt attcaagaaa ctggagataa atttagagtg atagaataaa
    18781 attttacata atttaatatt ttcaaaacat taatgaagga tatacctttg ttattagtag
    18841 tattaggtgc tactgctaca ggaaaaactc gcttagcagt taaaatagct attgaaataa
    18901 atggagaaat tatcagtgct gatagtagac aggtttataa agggttagat ctgggtacag
    18961 gcaaagattt gaatgaatat aaaataaata ctacagatat tccctatcat ctaattgata
    19021 ttagatctat tgatcaaaca tatagtgttt atgattttca gagagatgct ctagtagcta
    19081 ttgaaaaaat tataaataaa aataaaactc ctattatatg cggcggtaca ggcttgtata
    19141 ttgaagcatt attattaaat tataaattta ataaatttaa tcctgatgta gaattaaggg
    19201 aaaaactttc taagatgagt atacatgagt tagatcttat tctaaagcaa ttagatagca
    19261 atataatagt agaaaaaaat aatcgacacc gttacattag agcaatagag attgcactac
    19321 aaaataataa taatagcaat gaacaggata ttttagaata taatataaat aaatcattgg
    19381 tttttggtat tagatatcct agagatatgc tgagagaacg aatacataaa aggcttttgg
    19441 atcgtattga ggccggcatg atagaagagg taaaaatatt attagaaaat aatatagatc
    19501 ctaataaact tattgcattt ggattggagt acaaatttat cacaatgtat cttttaggaa
    19561 aattgactta cgacgaaatg atagataaat tatacacagc tatatgccaa tttgcaaaaa
    19621 gacaagaaac ttggtttaga agaatggaga aaagaaacat aaatattaac tggatagatg
    19681 gaaggctaga agaagagaaa aaaataaaag aaatattaaa taaaattaat aatgaaatat
    19741 aaataatggt tctataacgt tttgagtagg taaagattaa aaaaagagta attttataaa
    19801 aaatttgtta atattagttt attaattata gtgtagttaa atattaacca caaagttctc
    19861 aaagaatgca caaagaacac agagaagaat gaaattaaaa ggactataaa tggcactttg
    19921 tggactttgt gtaataactt ggtgttctct gtggtaaaaa aaatcaacct aaataattaa
    19981 aaaataatta tttttttgta acaatataaa attttattac ccacaataaa cgttatagaa
    20041 ccatgtaaaa ttagtttatt attctattct aaaaaaaata ttttgaaaat tttataagta
    20101 attaaaaaag ataatagtaa aaaaaaaaat taatcattaa gatatttgtt tagcaaacaa
    20161 tcaattttta acatatcctt tcagagtcag tactacaata ctatgctcgg gatcattagt
    20221 aataactgta acagattttt gttgatttcc ttgtctgcca gcagtattga atgtgacttt
    20281 aatactagat ttttcgcctt ttttaagtat atttttttct ggggccacag cagtgcatcc
    20341 acagctagat ttcactttta atatttttaa gtcattttta ccttcatttc taaaaggaaa
    20401 ttctacaata actgattctc ccattttaat tgtatcgaaa acaacctcgg ttttttcaaa
    20461 aactatttta ggagcattag ttttgtcttt ttcagtcatc cagctaaaat cttgtattat
    20521 attagcactc acatacattt gtttaaccgg ttgatctata tcatctgttt ttaatctaaa
    20581 attatcaaaa acataaccat aatcattacg tgctttagca tcatatgaga taggtatctt
    20641 aagttcttga tcaggggcta tagtaatgcc acctgcaggt atgtgtattt ttaaaaatga
    20701 aggtaaattt tcagagtaaa tagttatttt tctatcactt ggattgtata catatatagt
    20761 atcagtcttt atttcggtat cctttatatc attaaatgta atatgattgg atttgaaacg
    20821 taaattgcct agtacctgtg gataattatc agctttggtt ttaggtttag ggattacgct
    20881 ccctttaatg gttaagacta catttggatt gtcaggatca tttgttatga ctgttacaat
    20941 tttattaaaa ttacctggcc ttcctaatgg attgtaagct acatctacat agccactttg
    21001 tccaggtagt acgggggttc tagtatagtc agaagtagtg catccacaag atgctcttac
    21061 atttagtatt tttaaggtgt cattacctat atttttaaat gcaaatctag ctgtttttct
    21121 cccatcttct tcttttattt gcccaaaatc atatactcta ctttcgaata aaattgatgg
    21181 tttcttttga gcatcaatga ctcctatact tattaatgtc attaataata aaactattaa
    21241 ttttttcata ttttttttat ttaatgcaaa tttaaaataa aaaaataaat tcaaaagaag
    21301 tttaaaaagc aaaataataa tctcaaatta ataaaatttt tatacatatg atataaaaat
    21361 gtagatttta tgtattaaaa ttatttttaa atacgaattt aattaattta aaaatcaaaa
    21421 accaggcaac tgaaacataa taggaggtat gtgcactaag gcccctatga tgaaaaggat
    21481 aagaataaat ttccaaatat atttgatcca cacattatat tgtattttag ccataccaag
    21541 tacacccatt agtacaccgc taacaggagt aatcatgttt gtgaaaccat cccctaattg
    21601 aaaggctaaa acgcctaatt gtctcgataa acctataata tcgcaaagag gagctattat
    21661 aggcattgtg agagcagctt tagcagagcc agaaggtaaa ataaaattca aaactgtttc
    21721 gaagatatac ataccttgaa ccgctaaaaa tttagaattc tctggaataa cattggcagt
    21781 ggcatatagt atagtatcca tgattttgcc atcttctaat atcactatta tagctgaagc
    21841 caaaccgact atcaatgctg ctgatgctat atctttagct ccatcgatca tagttttagc
    21901 tatatcattg gctttcattc tcaagctcat acctgtgaat atacctaaaa ctaaaaataa
    21961 agtagctatt tctttgatat accatttcca tactaataca ccaaatatca tagaaataat
    22021 ggcaaatccc agcaaaaaca atgcatatat ttccttttta tatttaatca aaataaatcc
    22081 acctaagact atactaaata ttatcataca aaatagtatg atagcttgcg atgaccataa
    22141 ttcattattg tttaatattt ttactaattc taaaatcata aaagcattaa taagtatcca
    22201 agtaatccaa ccacgccaat ctactcgttc gctcacatca tttatgtctt ttacacttgc
    22261 atttctccaa tattcatcag tatcatatac taatgatgcc ataggatttt tattaggtgg
    22321 tagtgtgata tattggttta gtggagccgc taatcatgct gtaaatgcag gtgctggcaa
    22381 agctgttaat tatatcaagc aac
//
LOCUS       DAOPRN010000031        22370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1931_length_22370_cov_130.614654, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000031 DAOPRN010000000
VERSION     DAOPRN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22370
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1931_length_22370_cov_130.614654"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PKI65_04925"
     CDS             <1..717
                     /locus_tag="PKI65_04925"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77817.1"
                     /translation="KEKMLDYIRCMLEGKTLRACAKEVGISLPTSFAWRHRILAALRN
                     FEANVNFFGIVEVEELLMNYSEKGRRYRNEEELAEAREKKKKKVAVIASKDRTGNMLF
                     KQLGLGKIKREQIEQLLNTKVSANSVICFGHNDEFRKLKSQNLERKEIVIDRVRKRGV
                     YSVATIRRHVNTFVGWINNQFRGVATKYLQSYIMWYVVMHKYLLNNVVPDVGRMLNLA
                     SSDRRAWYVYRDLRASPVYI"
     gene            972..1520
                     /locus_tag="PKI65_04930"
     CDS             972..1520
                     /locus_tag="PKI65_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77818.1"
                     /translation="MKKWINIVIFTLICFIINSQNLLDIPKLDSLNINWYYDKNMAPY
                     SFENGIIITPPPYFLPFKYTNKQGFINPKFLSSIEVEKINNVPYTILAKKIKENLEEQ
                     NIFLINVEKNFTNSDKSCLIYEMIYNAMTSEGHNIDFHQIILVTGDYNTSIIGIAVFP
                     EMYYKLMHNVMRESLLSIDCEN"
     gene            1525..4320
                     /locus_tag="PKI65_04935"
     CDS             1525..4320
                     /locus_tag="PKI65_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOB77819.1"
                     /translation="MKKYITIYFIFNLLIITHINAQTYVVTHNINWFSHNQDMWGPGG
                     TPIIMDQDINFFDVEFGPYSTTIGGITDMGLLGEWGAELDLDAWFRLGSHLGIHGFTT
                     GYVNVDYPVRIRMTIPNNNTFCPGDTLKIHSQYDILTGWNLNTYFPEAGVIGLYLDFG
                     FNLDFDATICVYSCFDASIIDVNIPYDTIPILELNSLTGVFTYPCFDPGSFPPITICH
                     NQILPIIFDVPIIGLTGSITLPYVETHDWKDVVDVCEQNLYAQGSNTWINLGIDVIQI
                     LSTLAGFIPPPAGPAIQSFLAILDGSIDIAIVHIQYSLFSAYFTIKSTMIQNFSFKPK
                     IWNKLSFPTPIEYFVTDPTMNDSIIEQNISNEIDFLACQDLYFKWPCHDYTDMNIDIM
                     HRLTNEFSNHTYDSISFDFTISALEFWIDINFKNSEIISIIPDLCIDLPSDPDDTTYQ
                     NSIVCIIPTVLDDIILQPSKLSIHIGPLFSQTFPLGHLYLTWIDETWELAGFNDTIFP
                     SINMNTDCPGLEAHNLIAQHISCYGDSSGSISISANGGVPPYNYIWSTGDTITTNDTI
                     DIIDNLPAGWYYVTVFDKNQCFITDSTELINLYPPLVVHPFVSNVTCEGGSDGSIVLT
                     PTGGHPLYTYLWSNQATGPINNNLPAGDYSVTVTDSEGCDTTMNFNIIELYPLPPVNI
                     NVSPLDGCQPLLITAKEVSPNQGQSYLWNFGDGTFSNDKYTHHLYSREGNYHITCEVT
                     SIHGCIDSLSTNVTVFYKPKADFHYLPTNLDLENNFVHFINNSDNTYHSIWDFGDESM
                     SAETSPFHSFADTGIYVVTLYITTEDGCMDTIYKTLYIGDISAFYIPNAFTPDGDGIN
                     DIFMPKAQNILSDNYEFRIYDRWGKMLFKTTQIGIGWDGKYEGEKCDPGVYTYYIKYL
                     NEKKLKTEKWGVVILVR"
     gene            complement(4578..7037)
                     /gene="pheT"
                     /locus_tag="PKI65_04940"
     CDS             complement(4578..7037)
                     /gene="pheT"
                     /locus_tag="PKI65_04940"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOB77820.1"
                     /translation="MIVSLNLIKQFINPWQVSNEEVYDILNSVGLEVEQEIEYENIPG
                     LLNHIVVGEVSSVEKIEGTDHLHKTLVNIGNGLTKTIVCGAPNVAIGQKVVVALDGAL
                     VKINNEWIKLKKTKIRGVQSEGMICAEDELGIGESHEGILVLPNNIPIGEKANLLFKD
                     LLYQDLIYQIALTPNRADAASHLGVARDIYAAFKQRYQQKEMIFITPEVIDIKPSKYL
                     PISIEIKNPEACPKYCGIVIDNVKVKDSPSWLKNYLNAWGMKSINNIVDISQYIMMEI
                     GQPLHIFDYDKIIGNKLIIKNVEKGTKFKFLDGIERELSEGDLMICNTDEPMCMAGIM
                     GGMYSGVTYTTKSIFIESAYFDPTYIRRSARRHNLHTEAAYRYERGVDYSILEYAIKR
                     AAKLILEVAGGEIASDIVCDIAKLIKPTIITFSLNNINKFLSTNIIKDNAKRILHDLN
                     FNLLDDDGDILTVEVPMAKVDVKQSVDIAEELIRIIGFDKIESSKRANFFMQPYKPSF
                     HSFIYNLSIKLKAEGFTEIMNNSMESDKVYNKFPFFADEQYIKVLNPISNELNILRPS
                     LMFGGLKTLIYNLNRKQKNLFLFEVGKVYKRNKSDAIDVKEKFEEQLQLAIWMTGTLF
                     PENWKRPNDKIDLFDIKAIVENILKHTLNNNLHDIKEVEHNFLLNSFQFIYDNDVLAT
                     VGEVRKDILRYFDIQDQVFYAEIYLETLYDLFKQNKIKYTEPNKFPIVRRDLSLLLNK
                     NITFKQVKDVIYETVPKCIKAVNLFDVYEGKNLPDDKISYAISIIFEKKQATFTDEEI
                     DNLMKKIIEALNSQLGIVLRG"
     gene            7192..7539
                     /gene="secG"
                     /locus_tag="PKI65_04945"
     CDS             7192..7539
                     /gene="secG"
                     /locus_tag="PKI65_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOB77821.1"
                     /translation="MGGAVLTFMIIAIIVAILLAVIILIQNPKAGFAANFSVANTMFG
                     ARESANKLEKATWIMAIMILVLSIASSIAMPKGEQIKGSQVKEAIEQTQLPVNIPQSP
                     APLSPQLPDDGDN"
     gene            complement(7634..8968)
                     /locus_tag="PKI65_04950"
     CDS             complement(7634..8968)
                     /locus_tag="PKI65_04950"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOB77822.1"
                     /translation="MKIEKTTTENNLSDILINLEPQDYNEEYHKKIKQIRQSVNIPGF
                     RPGKAPLSLIEKRYKADVLSEEIIKKAYDLFYQYINENKISYVGKPIMKFDNFNLDKD
                     TEFAINFQIIELPHLDIDLPQDLELTKYNIIVNDEEINDVIDSMTNKMGQIQDVDSFS
                     EADYVLLDFEFELDDSKINKFVSIEPKKEKKLKLDALKDITIDNSIEINVLDVFNTFE
                     EANEILFPNEQQKLDTDKKIKLSLKFAYKIEPAPIDQVLFDNVFPSRNIQSEEEFRQS
                     IAEVVEKSHKADTINSIIKQLTDYLLENYPFDLPKDILYEQMVQINNEQENGLTPEQI
                     SDNFDVFVENIKREHYLNIIAVKYGINIEFDDYKNLIASEYNISSDDLENDEEKKQLV
                     DYMLEQLSKDSDALDDARNVILVNKVYEIVLDKCNIVEKEITYLEFSELNKN"
     gene            complement(8990..9071)
                     /locus_tag="PKI65_04955"
     tRNA            complement(8990..9071)
                     /locus_tag="PKI65_04955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9035..9037),aa:Leu,seq:tag)
     gene            complement(9228..9779)
                     /locus_tag="PKI65_04960"
     CDS             complement(9228..9779)
                     /locus_tag="PKI65_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOB77823.1"
                     /translation="MNYNKKNIEELNRLSIEQYKTVKKLPIYLVLDNIRSKYNVGSIF
                     RTADAFRIEKIFLCGITPCPPDREIEKTALGATKSVDWQYLSDTLSCAKLLKDQMVFL
                     LAVEQTFNSTSIINLPFNLPLPIAIILGHEIKGVDDKILEMVNIAVEIPQWGTKHSLN
                     VSVAAGITMWEVVKRNIDTVESK"
     gene            complement(9786..10739)
                     /locus_tag="PKI65_04965"
     CDS             complement(9786..10739)
                     /locus_tag="PKI65_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOB77824.1"
                     /translation="MIKKIKHYLDTHYYPKTDKSYTFLIETFNKGVQNSLLGVRAAAI
                     AFKLIMAAIPLLIVILNLLPSFFTIDNLSSFLILLEEYLPSNVYNVIDGPIKYILFDT
                     TRGYTGIWMFAALFIALNSVRLIMESFNASYHIKETRPIWEQFFTAIIVWLLLTLLLV
                     ISISLFLTNKNFFQWLVSLNLIKPGVIRSFYFVAKYILFFILLIILIEVIFYIAPARR
                     RKFRFMTPGTIIAALSTLIISGGFDFFITHFSNFNKIYGPLATIFIFFLWLQTVAFMI
                     LVGFEINAVVYLADDDHPRPILPKLNFKKLFTKSSKKSKKS"
     gene            complement(10822..11463)
                     /locus_tag="PKI65_04970"
     CDS             complement(10822..11463)
                     /locus_tag="PKI65_04970"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like putative outer membrane lipoprotein
                     chaperone"
                     /protein_id="HOB77825.1"
                     /translation="MKNIKTFFIILFLPFALVSQVIDPRAQQILDESYNKIKNYKSIQ
                     IDFTYTIKNEEKNTRQQNKGSIMLKGNKYKLVLDDQIIFCDGSTKWTFFVDDNEVQIN
                     NVDVNEELLPNYILLADYKKDHYPKFIREMPKSNKVINIIDLVPKTSKSYYKIRLEID
                     NNTKLLNSASIYNKDNNTFIYVIDRFITDIDAPDSIFVFNPKDHPGVFINDMR"
     gene            complement(11480..13900)
                     /locus_tag="PKI65_04975"
     CDS             complement(11480..13900)
                     /locus_tag="PKI65_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOB77826.1"
                     /translation="MTQKKENKIINDKKKAKNTVKAKQSTKKSTKSSDTTDNNIFKTN
                     FRSIIYWICLSLILLSAIYIFLSIIFYFFYGVADEPFLINGFSSISDINFHSSNPLGN
                     FGAWIGYELVKQGFGIAAFLIPIWLFLLFYSLAKNNFSIFLKYQTHTLIPLILLPLFL
                     SLFGDIAGGLWGTKVKNLLTPYIGTFFYAIIAISINVAYFFWLFSISYKKLFANVLSI
                     TKIKSKKSSNDKQPLIQDKEAIINNDSQVNIEESQLQYAWDINDSNIVSDNKIKSKKD
                     KTIKDDIEFTIEENKNTITVENITNETEKQQEFIPEIYNLDYQLPPLDILKYYEKENN
                     KVSIEELEANKNKIIETLAQYQIQISKITATVGPTVTLYEIVPAPGIRISRIRSLEDD
                     IALSLAAYGIRIIAPIPGKGTVGIEVPNSKKEIVSIRTVLESEKFKNFNGELPIVLGK
                     TISNDVYVTDLTKMPHLLIAGATGQGKSVGLNTIITSLLYKKLPHEVKFIFIDPKKVE
                     LAPYSLLEHHYLAKIPKEEEAIITDVSKVVETLISLTILMDVRYELLKMAGVKNIIEY
                     NKKYLSGKLDPELGHEFMPYIVLVIDEFADLIMTAGKEVELPIARIAQLARAVGIHLI
                     IATQRPSVDVITGKIKANFPVRIAFRVSSKVDSRTILDMNGAEQLIGRGDMLIAVGTE
                     VIRIQCAFIDTPEIDALMEYISQQPSFPEAYLLPEPIIEGTEYKIIDVQSLDPLLLDA
                     AAIIITSQSGSTSLLQRKLQIGYNRAGRIMDQLEALGIVGPPAGSKPREVLIADITQL
                     EQILNNMN"
     gene            complement(14192..14884)
                     /locus_tag="PKI65_04980"
     CDS             complement(14192..14884)
                     /locus_tag="PKI65_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77827.1"
                     /translation="MKKLQFAFFSVLIITSLTAFGQKSKSSNKPFAAEITYKIEYQGN
                     WDPAVVAQQPKELKVYVYGNKMKTVMDLSGMGYITTIQNGDDTTNIILIDIPLAGLKK
                     YVKISKENILDKFEESQPEIRYIDSTKEIAGYKAYKAEYIVKDEFGDPDLTVVWYSPE
                     INDGQINASGDFAGLKGFPMEYYSSEDDAIIVFRVTEVKTSKVKLKDTDFLIPSDFDE
                     LTEEEAKELLGQ"
     gene            15027..15389
                     /gene="rplS"
                     /locus_tag="PKI65_04985"
     CDS             15027..15389
                     /gene="rplS"
                     /locus_tag="PKI65_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOB77828.1"
                     /translation="MGRDDLIRYVEEQLNTKPDFPEFRAGDTITVHYKIKEGNKERIQ
                     QFQGVVIQIKGKGLNKTFTVRKISNNVGVERIFPFTDPFIAKIEVNKRGRVRRARIYY
                     FRKLRGKKAKIKERRIVS"
     gene            15550..15861
                     /locus_tag="PKI65_04990"
     CDS             15550..15861
                     /locus_tag="PKI65_04990"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOB77829.1"
                     /translation="MTWKNFFRKIDKYKYLIALLIFLILIIFLDNNNLIKLRKYKKEL
                     NQAKEQNEYYKQKIVEDSLEMQRLKKDKEQLEIYARKTYFFKKDNETIFLIVKDSTKT
                     E"
     gene            complement(15876..17033)
                     /locus_tag="PKI65_04995"
     CDS             complement(15876..17033)
                     /locus_tag="PKI65_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOB77830.1"
                     /translation="MKLNNFDDIRSFRDNEVNHYLKIVVKDPLFLEILAYQFGKENLS
                     WWLESISKINTIKELQTTLLKPLLENLLQKSVSELTYSGLEYLDKNESYLFISNHRDI
                     LLDSFILNYIFFINGFETIESAIGNNLLVMPWIEYLARLNKTFIVQRNLEGKKFYYAS
                     HKLSQYINDCLLNRNQSIWIAQQEGRSKDGNDITQSSLIRMILLAADKGKEIELLNKY
                     KIIPVSLSYEYDPCVIYKIIDNYLHSHGKTFDKSDEFRLNEMKEGLIEYKGNVHFSIS
                     KPLSFKKNNQNLKDLIKLVTSQIDKEIHKNYIIYPFHWYCYDKVNNSSENIEKYYGKE
                     NEFENYINNQINKLKIFTKNELDNVDAMILCIFKFYAQIVENYLKACSFSL"
     gene            17106..18725
                     /locus_tag="PKI65_05000"
     CDS             17106..18725
                     /locus_tag="PKI65_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOB77831.1"
                     /translation="MKKELFFLINFFVVISLFAYPGEIIKQLKAPNRHCTGLTYDGKY
                     LWVADRGTDSLYKIDTKDGHIIKSISAPGYWTTGLAWDGQHLWVIDERGGIPQGDEFF
                     TGYLYMIDTMNGNVLHHTSLPFSNPQDLTYDGKYMWVISATDNKLIKFNPYDGTSIIE
                     FTAPSRGCTGLSYDGSYLWIANHIRNEIYKVSPIDGSVIILTYSPNEYPRGMAFDGNY
                     LWLVDSQTDSLYQMKIRDDDKLIKTKSRTAKLVFTQSAMNFGPGKVNTLDFYFAIPQS
                     MDNQEILGEVQFSTKPTKILTDKWGQKIAHFHYDNVSAGTKVQIEMYVDAIMYDTRYF
                     IYPEKVGTLNDIPEKLKKIYLVDDEKYDITHPTIQKAVQEAIGNELNVYKIVKNIYHY
                     LHDKLYYERAGGWNTAPTVLERGNGSCSEYTFVFIAMCRAAGVPARYVGSVVERGEAA
                     SMDEVFHRWVEVYLPNYGWIPVDPSGGDEPLQADKARYFGFTGRRFLITTTGGGNSEY
                     LDWSYNGNVRFTTEPKTFVVTESYGDWEPIY"
     gene            18773..22231
                     /locus_tag="PKI65_05005"
     CDS             18773..22231
                     /locus_tag="PKI65_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HOB77832.1"
                     /translation="MKKLFSLVLLILISSFINSQEYKKVEAEKIMQGTEHIYYNSNKT
                     FPTYIKFSSDISKSIDQILSEWQKNYNLSYKLLNVEKDLLGFEHYKYQQYYDTIPVEW
                     AIFKIHVKNEKVESYSGTIWLNLQAKTNPVISKNKAIEIAIIEHNADVYMWQDKDEEK
                     YLKMMTKDENATYYPKPQLVIYKTADKEVLAYKLTIYSKYPHSKKDYYIDAEKGNIID
                     VIDKIHHTDTQGTAVTKYSGIQPIITDYYNGYYRLRESGRGNGIFTFNMQMGSNYYNA
                     VDFTDDDNYWNNVNPQKDEVATDAHWATEKYYDYFLNTFNRNSIDNNGFALYNYIHAN
                     LIEMGLSSNVNAFWNGYYMTYGDGDAMHSPLTTVDIIAHEITHGLTEFTAGLVYAGES
                     GALNEAFSDIFGTVVEFYAKPNTANWTIGEDIGSAFRSLANPNYYENPDTYFGDYWDL
                     NNEVHNNGTVMSHWFYIVVNGKTGANDLGNNYSVTGIGMEKAAKIAYYTLVNYLPPNA
                     TYYDARFFTIVVATNLYGACSAEVQAITDAMYAIGVGEPYQPQVIANFTANITENCEP
                     PLTVQFINQSNNGMSYYWDFGDGYTSTNISPTHTFNNYGNYTVKLIADGDLCGTDTLI
                     MANYISIAPDKMCHVVMPLNGYGGIITHCHGIIYDGGGPNGNYDDMSDARITISPPNA
                     TSITLFFNSFDIEPGSSSYCNYDYLEIFDGPNTNSPSMGRYCNTTGAPDTIISSGGQL
                     TLLLHSDQALNLSGFEAVWVCNAPDLAPLADFSIDSINKCNGFVKFIDRSFHNPTSWL
                     WDFGDGTTSNLKSPIHIYNDNGDYNVSLTATNNYGTDMITKQNIVNINRPAPPIVIND
                     TICENESATLIAIGNGVILWYSSYSDLYNFYNGSTYITPPLSQTTTYWVQDFFVPTTH
                     YVGDTRSSSNGGFFTNTNRHYLTFDCFTECTLVSVEVNASSAGNRLIELTDYQGNTIT
                     SKNVYIPKNISRVHLDFALPVANNLKLWGPGTPNLWRNNNGSNYPYQLNNILSIKNNS
                     AFDPSYYYYYYNWEIKLPDCASTKVPIVAFLDDCDNIENLLSTTIEIYPNPTSDKVYI
                     SLPDNETLKSIAIIDITGRVTSTNIFVNTNNNFYVDLSHLTDGIYLLQITIEDKAYLQ
                     KVIVQH"
BASE COUNT         7817 a         3252 c         3202 g         8099 t
ORIGIN      
        1 aaagaaaaga tgctcgatta cattcgttgt atgctcgagg gcaaaactct cagagcttgt
       61 gctaaagaag tgggcatcag cctacctaca agcttcgctt ggcgtcatag gattttagca
      121 gctttgagaa atttcgaagc caatgtcaat ttcttcggaa tcgtggaagt agaggaactg
      181 ttaatgaatt actccgaaaa aggaagacgc tatcgaaatg aagaagagct agcagaagca
      241 cgagaaaaga aaaagaaaaa agtagccgtt atagcatcta aagataggac tggcaatatg
      301 ctttttaagc aattaggctt aggtaaaata aaacgcgagc aaatagagca attgctaaat
      361 accaaagtat cagctaatag tgtgatatgt ttcggacaca atgatgagtt tagaaagttg
      421 aagagtcaga atcttgagcg aaaagaaata gtgatagata gggtaaggaa acgaggagta
      481 tacagtgtag caaccattag acgacatgta aatacttttg ttggctggat aaataatcaa
      541 ttcagaggtg tggctactaa gtacttgcag agctacatta tgtggtatgt agtaatgcat
      601 aaatatttgt taaacaatgt ggtgcccgat gtaggtagga tgctcaatct cgcatcatca
      661 gacagacgag cgtggtatgt atatagggac ttgagagcaa gtcctgtgta tatttaaaca
      721 ttgaaatgaa atctattgaa atctattgag atctattgca tccttctacc ccatcatatt
      781 accaatatat aattaaaata ttgttattat aaataaatat ttatccacaa taatttataa
      841 attttactat ttattcatat atcttatatg aattttttta tgtaattatt taaaaaatca
      901 atataattta tttaaatttg taaaattaat tttaaatttt tatttttgta taaaatattt
      961 ctatttttaa tatgaaaaaa tggattaaca ttgtaatttt tacattaata tgctttatca
     1021 taaattcaca aaatttatta gatataccaa aactagattc tttaaatata aattggtatt
     1081 atgataaaaa tatggctcca tattcatttg aaaatggaat aataataaca ccaccacctt
     1141 attttttgcc attcaaatat actaataaac aaggttttat aaatccaaaa tttttatcta
     1201 gtattgaagt cgaaaaaata aataacgtcc catatacgat attagctaaa aaaataaaag
     1261 aaaatttaga agaacagaat attttcttaa taaatgtaga aaaaaatttt actaactctg
     1321 ataaatcttg tctaatttat gaaatgattt acaatgctat gacaagtgaa ggacataata
     1381 ttgattttca tcaaataata ttggttactg gtgattataa tacatctatt attgggatag
     1441 ctgtttttcc tgaaatgtat tataaactta tgcataatgt aatgcgagaa tctttattat
     1501 caattgattg tgaaaattaa atttatgaaa aaatatatta ctatttactt tatttttaat
     1561 ctgctaataa ttactcatat aaatgcgcaa acttatgtag taactcataa tataaattgg
     1621 tttagtcata atcaagatat gtgggggccc ggtggcactc caataattat ggatcaagat
     1681 ataaattttt ttgatgtaga gtttggacct tattctacaa ccattggagg tatcacagat
     1741 atgggattat taggtgaatg gggtgcagaa ttagatttag atgcttggtt taggttaggt
     1801 agtcatttag gtattcacgg atttactact ggctatgtaa atgtagatta tcctgttaga
     1861 ataagaatga ccatacctaa taataatact ttttgtccgg gagatacttt aaaaattcac
     1921 tctcaatatg atattttaac tgggtggaat ttaaatactt atttcccaga agcaggtgtt
     1981 attgggttat atttagattt tggttttaat ttagattttg atgctaccat ttgtgtttat
     2041 tcttgcttcg atgctagtat aatagatgtt aatattcctt atgatactat tccaatttta
     2101 gaattaaata gcttgacagg tgtttttact tatccttgct ttgatccagg ttctttccct
     2161 cctattacta tttgccataa tcaaatttta ccaattatat ttgatgtccc aattatagga
     2221 ttaacaggta gtataacctt accttatgta gaaactcatg attggaaaga cgtagtagat
     2281 gtatgtgaac aaaatttata tgctcaaggt agtaatactt ggatcaattt aggaatagat
     2341 gtgattcaga tattatctac tttagctgga tttatcccac cacccgcagg accagctata
     2401 caatcttttt tagctatttt agatggtagc atagatattg caattgtaca cattcagtat
     2461 agtcttttta gtgcatattt tactattaaa agtactatga tacaaaattt ttcttttaaa
     2521 cctaaaattt ggaataaatt gtctttccct acaccaattg aatattttgt cactgatcct
     2581 acaatgaatg attcaattat tgagcaaaat attagtaatg aaatagactt tttagcttgc
     2641 caagacttgt attttaaatg gccttgccat gattatactg atatgaacat agatattatg
     2701 catcggctca ctaatgaatt ttcaaatcat acttatgaca gcataagttt tgattttacc
     2761 atttcagcac tcgaattttg gatagatata aattttaaaa attcggaaat tatttccatt
     2821 attcctgatt tatgcataga tctaccatca gatcctgatg atactactta tcaaaacagt
     2881 attgtatgca ttatacctac tgttttagat gatattatat tgcaaccttc taaactttca
     2941 atacatattg gaccactatt ttctcagact tttcctttag gacatttata tctaacttgg
     3001 attgatgaaa cttgggaatt agctggtttt aatgatacta tcttcccttc aataaatatg
     3061 aataccgatt gtccaggttt agaagcacat aatctaatag ctcaacacat tagttgctat
     3121 ggagatagct caggtagcat ttctatatct gcaaatggtg gtgtcccacc ctataattat
     3181 atttggtcaa ctggtgatac tataactaca aatgatacca tagatattat tgataattta
     3241 cctgcgggtt ggtattatgt gactgttttc gacaaaaatc aatgttttat tactgattct
     3301 acagaattaa taaatttata tccaccattg gtagtgcatc cttttgtttc taatgttact
     3361 tgtgaaggtg gtagtgatgg aagtattgtt ttaactccta ctggcggaca tccactttat
     3421 acatacttat ggagtaatca ggctacaggt ccaataaata acaatttacc tgcgggcgat
     3481 tatagtgtaa cagtgactga tagtgagggt tgcgatacta caatgaattt taatataata
     3541 gaattatatc ctttgcctcc tgttaatatc aatgtttcac cattagatgg ttgtcagcct
     3601 ttacttataa cagctaaaga agttagtcct aatcaagggc aatcatattt atggaatttt
     3661 ggtgatggta cttttagcaa tgataaatac actcatcatc tttattcaag agaaggtaat
     3721 tatcatataa cttgtgaagt aacatctata catggctgta tagattctct aagtactaac
     3781 gttacagtat tttataagcc taaggcagat tttcattatc tacctacaaa ccttgattta
     3841 gaaaataatt ttgtacattt tataaataat tcagataata cttatcattc tatatgggat
     3901 ttcggcgatg aaagtatgag tgccgaaacc agccccttcc atagttttgc tgatactggt
     3961 atttatgtgg taactctata tataacaact gaagacggtt gtatggatac aatttataaa
     4021 acattataca taggtgatat ttctgctttt tatataccta atgcttttac acctgatgga
     4081 gatggtataa atgatatttt tatgcctaaa gctcaaaata ttttatctga taattatgaa
     4141 tttagaatct atgatcgatg gggaaaaatg ctattcaaga ctactcaaat tggtatagga
     4201 tgggatggta aatacgaagg tgaaaaatgt gacccaggtg tatatacata ttatattaaa
     4261 tatttaaatg aaaaaaaatt aaaaactgaa aaatggggcg tggtgatctt ggtaagataa
     4321 atattgatat ttttagtaaa ttaatataaa tatatttttt caatggtaaa agggagaaga
     4381 taaaatgcta gaaaaaagtt aagaacagca gactaggaaa tggttgaatt tgagatttag
     4441 aatcaagatg aaaacaaaaa ttgcagtagc aatatattag caaaatccta ttaccactta
     4501 aatctaaaga aacagagata ttttctaaac ttttttttaa aatttaataa attaaagctt
     4561 ttatccaaaa ttcattatta ccccctcagt actataccta attgagaatt taatgcttct
     4621 atgatttttt tcatgagatt atctatctct tcatcagtaa atgtagcttg ctttttctca
     4681 aaaattatac taatagcata ggagatttta tcatctggta aatttttgcc ttcgtataca
     4741 tcgaataggt ttaccgcttt tatgcattta gggactgttt cataaatgac atctttaact
     4801 tgcttaaatg taatgttttt attcaaaagc aaagaaaggt cccgtctgac aataggaaat
     4861 ttatttggtt ctgtgtattt tattttattt tgtttaaata aatcatataa agtttcgaga
     4921 taaatctcag cataaaatac ctgatcctga atatcaaaat atcgtaatat atcttttctc
     4981 acctcaccta cagtggctaa aacgtcatta tcatatataa attggaaaga atttaataaa
     5041 aaattatgtt ctacctcttt gatatcatgc agattattat ttagagtatg tttcaagata
     5101 ttttctacga tagctttgat atcgaaaaga tcgattttat catttggtct tttccaattt
     5161 tcaggaaata gagtccctgt catccatata gctaattgca attgttcttc aaatttttct
     5221 ttaacatcaa tagcatctga tttatttctt ttataaacct ttcctacttc gaaaaggaat
     5281 aaattttttt gttttctatt caaattgtaa atcaaagtct taagaccgcc aaacatcaaa
     5341 gaaggacgta aaatattcaa ttcattacta ataggattaa gcacctttat gtattgttca
     5401 tcagcgaaaa atggaaattt attataaact ttatcagatt ccatactatt attcataatt
     5461 tcagtaaagc cttcagcctt caatttaata gatagattat aaataaaact atgaaaagaa
     5521 ggtttgtatg gttgcataaa aaaatttgct cgtttactac tttcgatttt atcaaatcca
     5581 atgatgcgaa taagctcctc agctatatct acagactgct ttacatctac tttagccata
     5641 ggtacttcca cggtcaatat gtcaccgtca tcatctaata aattgaaatt aagatcatgc
     5701 aatattcgct tagcattgtc ttttataata ttagtactca aaaatttatt aatattattt
     5761 aaagaaaaag taatgatagt aggcttaatc aatttagcaa tatcacatac aatatcagaa
     5821 gctatttcgc caccagctac ttctaagatc aatttagcag ctcttttgat agcatactct
     5881 aaaatactat aatctacacc tctctcgtaa cgatatgctg cttctgtatg cagattatgc
     5941 ctacgagcag atctacgaat ataagtggga tcgaaataag cactttcaat aaaaatagat
     6001 ttagtagtat atgtaactcc actatacatg ccacccatta taccagccat acacataggc
     6061 tcatcagtat tacatatcat cagatctcct tcagacaatt cacgctctat accatctaaa
     6121 aatttaaatt tagtaccttt ttctacattt tttataatta gtttattgcc aattatttta
     6181 tcataatcaa aaatatgcaa aggctgtcct atttccatca taatatattg agaaatatca
     6241 acaatattgt tgatgctttt cattccccaa gcattcaaat aattttttaa ccagctagga
     6301 ctatctttta cttttacatt atctatgaca ataccacaat attttggaca agcttccgga
     6361 tttttaattt caatagaaat aggtaggtat ttagacggtt ttatatcaat tacttcggga
     6421 gtaataaaaa tcatttcttt ttgctgataa cgttgcttaa aggctgcata gatatctcga
     6481 gctacgccta gatgcgaggc tgcatctgct ctattaggcg tgagtgctat ttgataaata
     6541 agatcttgat aaaggagatc tttaaataag agattggctt tctcacctat tggtatatta
     6601 ttgggcaaaa ctaaaattcc ctcgtgtgat tcgcctatac ctaattcatc ttcggcacat
     6661 atcatacctt cactttgtac acctctaatt ttagtttttt ttaattttat ccattcatta
     6721 tttattttca ctaaagcacc atctagagcc acgacaactt tttgacctat agctacatta
     6781 ggtgcgccac aaacaatggt tttagtgaga ccattaccaa tatttactaa tgttttatga
     6841 agatggtctg tcccttcgat tttttcaact gatgatacct ctcctacaac gatatgattt
     6901 aataggcctg gtatattctc atattctatc tcttgctcta cttctaagcc tactgaattt
     6961 aaaatatcat atacctcttc attagatacc tgccaaggat ttatgaattg cttaataaga
     7021 tttaaagaaa ctatcataag taaaaataat tttatttaat tgcaaatttc tataattttt
     7081 ttcattatag aaatagccaa tgtaaaaaat taatttattt tgagcaaaat aaattttata
     7141 aattaaaaaa ttagatgtaa atttgcaaaa aatataatat atattaaaag tatgggtgga
     7201 gcagttttaa catttatgat aatagctatt atagtggcta tattattagc cgtaatcata
     7261 ttaatacaaa atcctaaagc tggttttgca gctaattttt cagtagctaa tacgatgttt
     7321 ggagctcgag aatcagcaaa taaattagaa aaagctacat ggataatggc aattatgatt
     7381 ttggtattga gcattgcctc ttcgatagca atgcctaaag gcgagcaaat aaaaggtagt
     7441 caagtaaaag aagccattga acaaactcaa ctacccgtta atatcccaca gagtccagct
     7501 cctctatcac ctcagctacc agatgatggt gataattaaa aaaaaattaa taataacata
     7561 tgtacctact gagatatact acagaatgaa atattcagaa ttattaggat atagtttttt
     7621 aaaaaaaaaa tccttaattt ttatttaatt ctgaaaactc taaataagtg atttcttttt
     7681 ctacaatatt acatttatct aaaacaattt cataaacttt attaactaat atcacatttc
     7741 tagcatcatc taaagcatca ctatctttag ataattgctc taacatataa tctacaagtt
     7801 gctttttctc ttcgtcgttt tctaaatcgt cacttgatat gttatattca gaagctataa
     7861 gatttttata atcatcgaat tcaatattta ttccatattt cacagctatg atatttagat
     7921 aatgttctct ttttatattt tctacaaaaa catcaaaatt atcagaaatt tgttcaggag
     7981 tcaatccatt ttcttgttca ttatttattt gcaccatttg ctcatataaa atatcttttg
     8041 gtaaatcaaa aggataattt tctaataaat aatctgttaa ttgttttata atactattta
     8101 tagtatctgc tttatgagac ttttctacaa cttcagcaat agattgtcta aattcttcct
     8161 cgctttgtat atttctactc ggaaaaacgt tgtcaaataa tacttgatca atcggtgcag
     8221 gttctatttt gtatgcaaat tttaaactca gttttatttt tttatctgta tctaattttt
     8281 gttgttcatt tggaaataaa atttcatttg cttcttcgaa agtattaaac acatctaaaa
     8341 cattaatttc aatagaatta tcaattgtaa tatcttttag agcatctaat tttaattttt
     8401 tttctttttt gggttctatt gagacaaatt tattgatttt agaatcatct aattcaaatt
     8461 caaaatcaag aagtacataa tctgcttcac taaaagagtc tacatcttgt atctgcccca
     8521 ttttgttagt catagaatca atgacatcat ttatttcttc atcattgact ataatattat
     8581 atttggtaag ttctaaatct tgtggtaaat ctatatctag atgtggaagt tcaataattt
     8641 gaaaattaat agcaaattct gtatctttat caagattaaa attatcaaat ttcataatag
     8701 gttttcctac ataagaaatt ttattttcat taatatattg ataaaataag tcataagctt
     8761 ttttaataat ttcctcacta agtacatcag ctttatatct tttttctatc aatgacaaag
     8821 gagccttccc tggtctgaaa cccggaatat taacagattg ccttatttgt ttgatttttt
     8881 tatgatattc ttcattataa tcctgaggtt ctaaattaat taaaatatca gataaattgt
     8941 tttctgtagt tgtcttttct attttcattt ttattataat atttaaaaat gcggatgaag
     9001 ggactcgaac ccccacgcct ttcggcacca gatcctaagt ctggagcgtc taccaattcc
     9061 gccacatccg cttaaaatat tttgcaaaat tagtcaattt tttaaaaatt aaaaattttt
     9121 aattaaaata aaaaaatatt attcttaata gtacatctct tattaaagta tttcttattt
     9181 atctcatcta aattttaatc attttagatg tttgattttt attctcctta tttactttct
     9241 acagtgtcta tatttctttt tacgacctcc cacattgtta ttccagcagc tactgataca
     9301 tttaatgagt gtttagtgcc ccattgggga atttctacag ctatattcac catttctaag
     9361 attttgtcat ctactccttt tatttcatgt cctaatatta ttgcaatagg tagaggtaaa
     9421 ttaaatggta aatttataat ggaagtacta ttaaaggttt gctctactgc taataaaaaa
     9481 accatttgat cttttaaaag cttggcgcag gataatgtat cagatagata ttgccagtcc
     9541 acactcttag tagctcccag tgcagttttt tcgatttctc tatcaggtgg acaaggagta
     9601 ataccacaga gaaatatttt ttctatgcga aaagcatcag ctgttctaaa gatagaaccc
     9661 acattgtatt tactacgtat attatccaat actaaatata ttggtagttt tttgactgtt
     9721 ttatattgct ctatagataa tctattaagt tcctctatat tttttttatt ataattcata
     9781 aattactagc tttttttact ctttttagat gattttgtaa ataatttttt gaaattcagt
     9841 ttaggtagta taggtctggg atggtcatca tcagctagat aaactacagc atttatttcg
     9901 aaacctacta atatcataaa agcaactgtc tgcaaccata aaaagaaaat aaaaatagta
     9961 gctaaaggtc catagatttt attaaaatta gaaaaatgcg ttatgaaaaa atcgaacccc
    10021 ccactaatga tcaacgtaga taatgccgcg atgatagtgc caggtgtcat aaatctgaat
    10081 ttacgcctac gagctggtgc tatataaaat attacttcta tcaaaataat gagtaaaata
    10141 aaaaataaaa tatatttagc tacaaaataa aatgaacgta tgacgcctgg tttgatcaaa
    10201 tttaatgaaa ctaaccattg aaaaaaattt ttatttgtca aaaataaact aatagaaatt
    10261 actaataaca aggttagcaa aagccagacg atgatagcag taaaaaattg ttcccatata
    10321 ggtctagttt ctttaatatg ataagatgca ttaaaagatt ccattatcaa tctcacacta
    10381 ttcaatgcaa taaataatgc agcaaacatc catattccag tatatcctct cgtagtatcg
    10441 aataagatat atttgatggg accatctatt acattgtata cattactggg aaggtattcc
    10501 tctaataata ttaaaaaaga acttagattg tctatagtga aaaatgaagg taaaaggttt
    10561 aatattacta ttaaaagtgg aatagcagcc attattagct taaatgctat tgccgctgct
    10621 ctcacaccta gcaaagagtt ttgtacgcct ttattaaatg tttctatcag aaatgtataa
    10681 gatttatcag ttttaggata ataatgagta tctaaataat gttttatctt ttttatcatt
    10741 acattaataa cttagatcat acaataacac tttgctacaa tatctaaatt tttactcttt
    10801 tattcagtat aactctattt ttcatctcat atcattaata aaaacacctg gatgatcctt
    10861 tggattaaaa acaaagatac tatctggagc gtctatatca gttataaatc tatcaatgac
    10921 ataaataaaa gtattattat ctttattata aatagaagca ctattgagca atttagtatt
    10981 attatctatt tctaatctga ttttataata agatttagaa gttttaggta ctaaatcaat
    11041 tatgttaata actttattac ttttaggcat ttcacgaata aatttaggat aatgatcttt
    11101 tttgtaatca gctaaaagaa tatagttagg caaaagctct tcattaacat ccacattatt
    11161 tatttgtact tcattatcat ctacaaaaaa tgtccattta gtagagccat cacaaaaaat
    11221 tatttgatca tctaaaacca atttatattt attacccttt aacattatag aacctttatt
    11281 ttgttgtcta gtattttttt cttcattttt tatagtatag gtaaaatcta tttgaatgga
    11341 tttataattt ttaattttat tatatgactc atccaaaatc tgttgagccc taggatctat
    11401 aacttgagaa actaatgcga atggcagaaa taaaataata aaaaaggttt taatattttt
    11461 cataatattt attttttttt taattcatgt tattcaaaat ctgttccaat tgtgttatat
    11521 cagcaatcaa aacttcacgt ggtttactac ctgcaggtgg tcctacgatg cctagagcct
    11581 ctaattgatc cattattcta cctgctctat tatatcctat ttggagttta cgttgtagga
    11641 ggctagtaga accagattgc gaagtaataa taatagctgc agcatctaat aacaatggat
    11701 ccaaactttg tacatctata attttatatt cagtcccttc tataataggt tcaggcaaaa
    11761 gatatgcttc agggaaactt ggttgctgag aaatatattc cattagagca tctatttcag
    11821 gcgtatctat aaaagcacat tgtatacgta tcacttcagt accgacagct attaacatgt
    11881 ctcccctacc tattaattgc tctgcgccat tcatatctag aatcgttcgt gagtctactt
    11941 tgctagaaac tctgaatgca attcgtactg gaaaatttgc cttaatttta cccgttatta
    12001 catctactga tggtctttga gtagctatta tcaaatgtat accaacagct ctagctaatt
    12061 gagctatacg agctataggt agctctactt ctttgcctgc ggtcattatc aaatctgcga
    12121 actcatctat tacaagaaca atatatggca taaattcgtg tcctaattca ggatctaatt
    12181 taccagataa atatttttta ttgtattcaa taatattttt taccccagcc atttttaaaa
    12241 gttcatacct gacatccatc aaaatagtta atgaaatcaa cgtttctacc actttgctta
    12301 catctgttat gatggcctct tcttcttttg gtatttttgc aagataatga tgttcgagta
    12361 aactataagg tgctaattct acttttttgg gatcaataaa aataaattta acttcgtgag
    12421 gtaatttttt gtataataaa gacgttataa tagtattcaa accaacagat ttaccttgtc
    12481 ctgtagcacc agctattaaa aggtgtggca ttttagttaa gtctgttaca taaacatcat
    12541 tagaaatcgt tttaccaaga actattggta attcgccatt gaaattctta aatttttcac
    12601 tttctaaaac tgttctaata gatacaatct cttttttaga atttggcact tctataccta
    12661 cagtcccctt acctggtata ggagctatta tacgtattcc ataagccgcc agtgaaagtg
    12721 ctatatcatc ttctaagctt ctaatacgcg aaattcgaat acctggggct ggtacaatct
    12781 catataatgt aactgtaggg cccacggtag cagtgatttt actgatttgt atctgatatt
    12841 gagctaaggt ttctattatt ttgtttttat tagcttctaa ttcttctatg gaaaccttat
    12901 tattttcttt ttcataatat tttaatatat ctaatggtgg taattgataa tctaaattgt
    12961 atatttcagg tataaattct tgttgctttt cagtttcatt tgttatgttt tctacagtta
    13021 ttgtattttt attttcttct atcgtaaatt ctatatcatc ttttatagtt ttatcttttt
    13081 tagatttgat tttattgtct gatactatat tgctatcatt tatatcccat gcatattgaa
    13141 gttgagactc ttctatattg acttgagaat cattatttat aatagcttct ttgtcttgta
    13201 ttaatggttg tttgtcgttg ctagattttt tggattttat ttttgtaata gacaaaacat
    13261 tagcgaataa ttttttatat gaaatactaa aaagccagaa aaaatatgct acattaatac
    13321 taatagcaat gatagcataa aaaaaagtac ctatgtatgg tgtcaataaa tttttaactt
    13381 tagtgcccca taaaccacca gctatatcac caaataaaga caaaaatagt ggtaataata
    13441 tgagaggtat tagtgtatgg gtttgatatt taaggaaaat agaaaaatta tttttagcaa
    13501 gtgaataaaa tagcaaaaaa agccatattg gaattaaaaa agcagctatc ccaaaacctt
    13561 gttttactaa ttcatatccg atccaagctc caaaatttcc taatggattc gatgagtgaa
    13621 agttgatatc agaaatagat gagaatccat taattaaaaa aggttcatca gctactccat
    13681 aaaaaaaata aaatattatg gataaaaata tataaatagc agataataat attaatgata
    13741 aacaaatcca atatatgata gatctaaaat ttgttttaaa aatattatta tctgtagtat
    13801 cgctactttt ggtacttttt ttagtacttt gcttagcttt tacagtgttt ttagcttttt
    13861 ttttatcgtt tattatttta ttttctttct tttgagtcat aaaatcattt agatataatc
    13921 aaattgttac aaaattaaaa ataaattaca aattaacaaa tcacctataa gttgcaattt
    13981 acaattgaat atttcaaatt ataaattaca aacaactatg aataaatttt taaagtttta
    14041 gaaattaaaa atataaaacc atgcaccttc acagaatgaa atatcaaatt atctgcttac
    14101 acatcaccac agtttttata tcttttggac tccttaaact gattttttag aggataataa
    14161 taattccctc ttaacaagta agcaaatata tttactgtcc gagtaactct ttagcttctt
    14221 cttctgttaa ttcatcaaag tcactaggaa tcagaaaatc agtatcttta agtttcactt
    14281 tactagtttt gacttctgtg actctaaaaa caataatagc atcatcttct gaggaataat
    14341 attccatggg gaagcctttt aatccagcaa aatctccaga tgcatttatt tggccatcat
    14401 ttatttcggg ggaataccaa acaactgtaa ggtcaggatc gccaaactca tcttttacaa
    14461 tatattccgc tttatatgct ttgtatccgg ctatttcttt tgtagaatca atatacctga
    14521 tctctggttg actttcttcg aatttatcta aaatgttttc ttttgatatt tttacatatt
    14581 tctttaatcc agctaatggt atatctataa gtattatatt ggtggtatca tctccatttt
    14641 gaatagtagt tatatagccc ataccagaaa gatccataac agttttcatt ttgttaccat
    14701 aaacataaac ttttaattct ttaggttgtt gagctaccac tgctggatcc caattaccct
    14761 ggtactcgat tttatatgta atctcagctg caaaaggttt gtttgaagat ttactttttt
    14821 gcccaaaagc tgttaaagat gtgataatta atacactaaa aaatgcaaat tgtaactttt
    14881 tcataattta ttaaatttct gcaaatttat taatattttt tataataata aacaaactaa
    14941 aaaaataatt aaaattcttt taaaaaatat gcacagatga aaaaaaatgt ttaattttgc
    15001 aaacaatttt tttaaaaata ttagatatgg gcagagatga tcttattaga tacgtagaag
    15061 agcagttaaa tactaagcct gattttcctg aattcagagc tggtgatact ataactgtac
    15121 attataaaat caaagaagga aataaagaga gaatacaaca atttcaaggt gtagttattc
    15181 aaataaaggg caaaggcctc aataagactt ttactgtacg taaaatttct aataatgtag
    15241 gtgtagagag aatattccca ttcactgatc cttttattgc aaaaatcgag gtaaataaaa
    15301 gaggtcgcgt acgtagggca cgtatttatt atttccgtaa attaagaggt aaaaaagcta
    15361 aaattaaaga aagacgaatc gtctcataaa aatatttatt taatatttta gtctttaaat
    15421 catttatttg tttacttaat ctttttttgt aaaaatagca ttgagtaagt gtttatcaat
    15481 tttatagtat ttttaaaaaa tcaaatacgt gtttattatt tttttaataa ttttgtataa
    15541 atcactctaa tgacgtggaa aaattttttt agaaaaatag ataaatataa atacctaata
    15601 gcattattaa tctttttaat tttaataata tttttggata ataataattt gataaaatta
    15661 aggaaatata aaaaagaact taatcaagca aaagaacaaa atgaatatta taagcaaaaa
    15721 atcgtagaag attcattaga gatgcaaaga ttaaaaaaag ataaagaaca gttagaaata
    15781 tatgcaagaa aaacatattt ttttaaaaaa gataatgaaa ctattttctt aatagtaaaa
    15841 gatagcacaa aaactgaata aaaaaataat taaattcata aagaaaaaga acaagctttt
    15901 aaataatttt ctacaatttg agcataaaat ttaaatatac aaagaatcat agcatctaca
    15961 ttgtctaact cattttttgt aaatattttt aatttattta tttgattgtt tatataattt
    16021 tcaaattcat tttcttttcc ataatatttt tcgatatttt cactagaatt attcacttta
    16081 tcatagcagt accaatgaaa tggataaata atataatttt tatgtatttc tttatctatt
    16141 tgtgatgtta ccagcttgat caaatctttt aaattttgat tattcttttt aaaactcagt
    16201 ggttttgaaa tcgaaaaatg tacattccct ttatattcta tcaagccttc tttcatttca
    16261 tttaatctga attcatcaga tttgtcaaaa gttttgccat gagaatgtag ataattatct
    16321 atgattttat agattacaca aggatcatat tcataagata aagacacagg aatgatttta
    16381 tatttattta gaagctctat ttctttacct ttatcagctg ctaacaaaat cattctaatc
    16441 aatgatgatt gtgttatgtc attgccatcc ttgcttctac cctcttgctg tgctatccat
    16501 atagattgat ttctatttaa taaacagtca ttgatatatt gtgatagctt atgtgaagca
    16561 taataaaatt ttttaccttc aagattacgc tgtacaataa aagttttatt taatctagct
    16621 aaatattcta tccatggcat tactaggaga ttatttccaa tagcagattc tatagtttca
    16681 aatccattaa taaaaaaaat gtaatttaaa ataaatgaat ctaatagtat atctctatga
    16741 ttagaaataa ataaatagct ttcattttta tctagatatt ctaatcctga ataagttaat
    16801 tcagatacag atttttgtaa taaattttct aataaaggtt tcaataaagt ggtttgtaat
    16861 tcttttattg tatttatttt agagatactt tctaaccacc aagataaatt ttctttgcca
    16921 aactgataag ctaaaatttc taaaaacaaa ggatctttta caactatttt tagataatgg
    16981 tttacttcat tatctctaaa agagcgaata tcatcaaaat tatttagttt cataattgca
    17041 aaattaataa aagaaaacaa ataatattgt ttttatttat tttttattaa atttgaaaaa
    17101 aaattatgaa aaaggaatta ttttttttaa tcaatttttt tgtagtaata tcattattcg
    17161 catatcctgg cgaaataatt aaacaattaa aagcacctaa taggcattgt acaggactta
    17221 catacgatgg caaatattta tgggtagccg atagaggcac agattcattg tataaaatag
    17281 atactaaaga tggacatata ataaaatcta tttctgcacc tggatattgg actactggct
    17341 tagcttggga tggccaacat ttatgggtaa tagatgagag aggtggcata ccacaaggcg
    17401 atgaattttt tactgggtac ctttacatga tcgatacgat gaatggtaac gtattacatc
    17461 atacatcatt acctttttct aatccacaag atctcaccta tgatggaaaa tacatgtggg
    17521 taataagtgc aacagataat aaacttatta agtttaatcc ctatgatggc acttcgatta
    17581 tagaatttac agctccatca agaggttgta ctggtttgag ctatgatggg agttatttat
    17641 ggatcgctaa ccatattaga aatgagatat ataaagtttc tccgattgat ggtagtgtaa
    17701 taatacttac atattctcct aatgaatacc cgagaggcat ggccttcgat ggcaactact
    17761 tatggctcgt agatagccaa actgatagtc tataccaaat gaaaatacgt gatgatgata
    17821 aattaataaa gactaaatct agaactgcta agcttgtttt cacacaaagt gctatgaatt
    17881 tcggtcctgg caaggtgaat accttagatt tttattttgc tatccctcag tcaatggata
    17941 atcaagagat tttaggagaa gtgcaatttt ctacaaaacc aacaaaaatt ttgactgata
    18001 aatgggggca aaaaattgca cattttcatt atgataatgt aagtgcagga accaaggtgc
    18061 aaatagaaat gtatgtagat gctattatgt atgacacacg ttattttatt tatccagaaa
    18121 aagtaggcac attaaatgat ataccagaaa aattaaagaa aatttatcta gtagacgatg
    18181 agaaatatga tattactcat ccaactattc aaaaagcagt gcaagaagcc attggaaatg
    18241 aattaaatgt ttataaaatt gtaaaaaaca tttatcatta tctacatgat aaattgtatt
    18301 atgagcgtgc aggcggatgg aatacagctc ctacagtact agaaagaggt aatggttcgt
    18361 gctctgaata tacattcgtt tttatagcta tgtgtagagc tgctggagta ccagctagat
    18421 atgtaggctc tgtcgtagaa cgtggtgaag ccgcttctat ggatgaagtt tttcatcgct
    18481 gggtagaagt atatttgcca aactatggat ggatacccgt agatccatca ggtggcgatg
    18541 aacctttgca agctgataaa gctagatatt tcggttttac tggtagaagg tttctgatca
    18601 ctaccactgg tggcggtaat tcagagtatt tagattggag ctataatggt aatgttcgct
    18661 ttacgactga accaaaaact tttgtagtaa cagagtcgta tggcgactgg gagcctattt
    18721 attaatataa tacacattga aaaccagtat taactagaaa ttaaaaaaaa ttatgaaaaa
    18781 attattttcg cttgttttat taatattaat atcaagtttt atcaactctc aagagtacaa
    18841 aaaagtagaa gccgaaaaaa taatgcaagg cacagaacat atctattaca atagcaataa
    18901 aacttttccc acttacatca aatttagttc agatatttca aaatcaattg atcaaatttt
    18961 atctgaatgg caaaaaaatt ataatctttc gtacaaattg ttaaacgtag aaaaagattt
    19021 attaggtttc gagcattata aatatcaaca gtattatgat actatacctg tagaatgggc
    19081 tatttttaaa attcatgtaa aaaatgaaaa agtagaatca tacagtggta ctatatggct
    19141 aaatttgcaa gccaaaacaa atccagtaat tagtaaaaac aaagctatag agatagctat
    19201 cattgagcat aacgcagatg tttacatgtg gcaagacaaa gacgaagaga aatatttaaa
    19261 aatgatgacc aaagatgaga atgctactta ttacccgaaa cctcaactag ttatatataa
    19321 aactgcagat aaagaggttt tagcttataa attaactatt tattctaaat acccacattc
    19381 taaaaaagat tattacatag atgccgaaaa aggtaatata attgatgtaa tagacaaaat
    19441 acatcatacc gatacgcaag gtacagcagt tactaaatat agtggaatac aaccaataat
    19501 cacagattat tataatggtt attatagatt gcgtgaatcg ggaagaggta atggtatttt
    19561 tacttttaat atgcaaatgg gtagcaatta ttataatgct gtggatttca ccgatgatga
    19621 caattattgg aataatgtaa atcctcaaaa agatgaggtg gctacagatg ctcattgggc
    19681 tacagaaaaa tattatgatt attttttaaa tacttttaat cgcaatagta ttgataataa
    19741 tggttttgcc ttatataatt atatacacgc taacctaata gaaatgggct tatcaagtaa
    19801 tgtaaatgct ttttggaatg gttattatat gacttatgga gatggtgatg ctatgcattc
    19861 accacttact actgtagata ttattgcaca tgaaatcaca catggattga ctgaattcac
    19921 agccggacta gtgtatgctg gtgaatctgg agcattaaat gaagctttca gtgatatctt
    19981 tggcacagtt gtagaatttt atgctaaacc caatacagct aactggacga taggtgagga
    20041 tataggttct gcatttaggt cattggctaa cccaaattat tatgaaaatc ctgatactta
    20101 tttcggtgat tattgggatc taaataatga agtacacaat aatggaactg taatgagtca
    20161 ttggttttat atagtagtga atggtaaaac aggtgccaat gatctaggga ataattattc
    20221 agtaactggt attggaatgg aaaaagctgc aaagatagct tattatacct tggtaaatta
    20281 tttaccgcca aatgctacat actatgatgc acgcttcttt acaatagtag tagctacaaa
    20341 tttatatggt gcttgttcag ctgaagtaca agctattact gatgctatgt atgccatagg
    20401 tgtaggtgaa ccttatcaac ctcaagtgat tgctaatttc actgctaata ttactgaaaa
    20461 ctgtgaacca ccattaactg tccagtttat taatcaatca aataatggaa tgtcttatta
    20521 ttgggacttt ggtgatggat acacaagtac taatataagc cccactcata catttaataa
    20581 ttatggcaat tatacggtta aattgatagc tgatggtgat ctttgtggta ctgacacttt
    20641 aataatggct aattatattt ctatcgctcc tgataagatg tgccatgttg ttatgccttt
    20701 aaatggttat ggtggtatta taactcattg tcatggtata atatatgatg gcggcggtcc
    20761 taatggaaat tatgacgata tgtcagacgc tagaataact attagcccac ctaatgctac
    20821 ttctattact ctttttttta attcttttga tatagaacca ggtagtagta gctattgcaa
    20881 ttatgattat ttagaaatat ttgatgggcc aaacactaat agcccatcta tgggtaggta
    20941 ttgcaataca acaggagctc ctgatactat aatatcatca ggcggacaat tgactttatt
    21001 gcttcattcc gatcaagctc ttaatttatc tggatttgag gcggtatggg tttgtaatgc
    21061 tcctgattta gcaccccttg ctgatttttc tattgattct atcaataaat gtaatggttt
    21121 tgttaaattt attgatagat cttttcataa tcctacttct tggctatggg atttcggaga
    21181 tggcactaca tcaaatctaa aatcaccaat acacatatat aatgataatg gagactataa
    21241 tgtttcctta acagcaacta ataactacgg caccgatatg attactaaac aaaatattgt
    21301 taatattaat agacctgcgc ccccaatagt tatcaatgat actatttgtg aaaatgaatc
    21361 agctacactt attgccattg gtaatggagt tattttgtgg tattcatcct attcagattt
    21421 atataatttc tataatggta gtacttatat tacccctcct ttatctcaaa ctactactta
    21481 ttgggtacaa gattttttcg ttccaactac tcattatgta ggtgatactc gcagttcaag
    21541 taatggtggc ttttttacaa atactaatcg acattatctg acttttgatt gcttcacaga
    21601 atgtacattg gtttctgtag aagtaaatgc ttcgtctgca ggtaatagac ttattgaatt
    21661 aactgattat caaggcaata ccattacttc taaaaatgta tacataccaa agaatatatc
    21721 tcgtgtgcat cttgactttg cattacctgt agccaataat ttgaaattgt ggggaccagg
    21781 aacaccaaat ttatggagaa ataataatgg tagcaattat ccataccaat taaataatat
    21841 tttatctatt aaaaataatt cggcttttga cccatcttat tattattatt attataattg
    21901 ggaaatcaaa cttcctgatt gtgctagtac taaagtacct attgtggcat ttttagatga
    21961 ttgtgataat atagaaaatt tattatccac tactattgaa atctatccaa atcctactag
    22021 tgacaaagta tatatctccc taccagataa tgaaacatta aaaagtattg caattataga
    22081 tatcactggt cgagtgacat ctacaaatat ttttgtaaat acaaataata atttttatgt
    22141 agatttgtct cacctaacag atggtatata cttgcttcag ataacaatag aagacaaagc
    22201 atatttacaa aaagtcatag ttcaacatta aatgtttttg ataagtgttg tttagaatgt
    22261 gtgttataaa taaatataac tggtatgatt aatttaccat ttttttaaaa aaaaattggc
    22321 tctacttaaa gaaaatgttg ttttcagata gccatatcga aattatgaat
//
LOCUS       DAOPRN010000032        22200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_1957_length_22200_cov_129.284805, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000032 DAOPRN010000000
VERSION     DAOPRN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22200
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_22200_cov_129.284805"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PKI65_05010"
     CDS             <1..980
                     /locus_tag="PKI65_05010"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77833.1"
                     /translation="TSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLKILSAPPL
                     CPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYIRCMVAGK
                     SLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSEKGRIYKN
                     AEELKRARKQKPKKVAVLAATDRAGNLLFKKFEGKKLSSEQIEQFLKAKMPKDGVMCS
                     SNKKAFKKVRKNVIKHKEIVTSNRQKRGIYSLSTVKKKASEFTNWIYFKFRGVATKYL
                     QNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVKLTYVN"
     gene            complement(1175..2485)
                     /gene="murA"
                     /locus_tag="PKI65_05015"
     CDS             complement(1175..2485)
                     /gene="murA"
                     /locus_tag="PKI65_05015"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOB77834.1"
                     /translation="MADFIVKGGKQLSGIVEPQGAKNEALQVICAALLTDEPVKLLNI
                     PLTRDIMNLLNLIHDLGIEVAIDRANNSVTICARNINLDMLEEEFIKEKQSLLRGSLM
                     LAGPILTKYKKAIIPKIGGDKIGRRRIDTHLFGFQKLGAEIKFDYFTNQVTLTANNGL
                     KGNYIMLPEASVTGTANIIMAAVLAEGTTTIYNAACEPYIQQLCKMLISMGAYIEGVG
                     SNLLKIYGVQKLHGTTHKLLADMIDVGSFIGLAALTQSNITIKNCNIPHLGIIPDTFR
                     KLGIIIIEDDDNLIVPHQDIYEVSQYIDGGILSISDAPWPGFTPDLLSIVLVAATQAK
                     GSVLIHQKMFESRLFFVDNLIEMGAQVILCDPHRATVIGLERRYPLRAITMSSPDIRA
                     GISLLIAALSAKGTSIIHNIEQIERGYENIDKRLQALGADIERK"
     gene            complement(2491..3093)
                     /locus_tag="PKI65_05020"
     CDS             complement(2491..3093)
                     /locus_tag="PKI65_05020"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOB77835.1"
                     /translation="MKYNSELEPLKISEYGRNIQKMVDYAKTIKDKEERTQFAHKIVK
                     TMASIVGNGKYNNQKYWDHLYILSDGELDIDWPVEVNIKYLHFVPERISYKEEYNSKF
                     RYYGRIIEEMINKAIEMPDGDEKNELLRHLIMTMKKLYMYWNNRMVTDDIIKNHINTL
                     SQGKLKIPEKIFDTTNVNIIKSSYESQKSKKYYNKNKKRR"
     gene            3355..6864
                     /locus_tag="PKI65_05025"
     CDS             3355..6864
                     /locus_tag="PKI65_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOB77836.1"
                     /translation="MDNYKSIAQLIDDIEKNILKINNLSHNNKFTKIDDLIGSLNDTF
                     LSYPILIDAFHQFNVLKNKFTQVRTLDHNDLLKLKNLFEIIIDNLKINTTKQNYLYTF
                     RNQIIDISIPQIVRKPYFNYQNLCHLRGIIEDIKTESDRLIITLYTISADGPFLDEIR
                     VHLFDHSKNNLLYPDLTQYGSILETGQDIFIQNCTYLKEKEYYSTADTLMVLYPDYIL
                     DVRDVSDVHIFDNSYPHFIRLIPIYSIIRTFVNINNVQKIQSILIGNLLGEIFDTYVA
                     YDRVEEAHLLTAYKDFIGKNIFELLYWYNNNTEGFNLSFLIQIIEDEYLPGLQLMLPS
                     IKRDKSMILYLEPFYISNLYGLQGRMDALAFTEKGNSANIYELKSHKPKNKGYLYTKS
                     HDAQLICYYLLFRSTYPEKKIFYKMAYPGAEERHLRSITFGENADERELMLIQEVLFA
                     RNNIVKAHLDYAKGDFSSLIRCMPKKSDTPSPNTISIFSNTETKLRDSQTFMYNDIII
                     MHNTLKAIADNKLLNAYFWGSVRFIYREMLSAKTGNPETTDIWESMIKESKWGYANIW
                     KKSLNKKFNQYEVLGPLIFKSFTPDNHLIFSLDNDDLSISRLREDDIIILYPYDENKN
                     SPLSQQLLKGYIIDINESKVKLSLANKFIPKKIFKAKSKWLAESDRWFHNYSYGIRNL
                     FYLISNDNKKFINLFLGIDRPEFVDVNQSIVGLETLDDSQAKAIKQSLSANDYFLIQG
                     PPGTGKTAKTLTTIAKNLYNNNVKTLIVAYTRRAVDEICNNLDKQSIPYLFLNKDLVK
                     NQLNTANNLNDLDNILPDLNNNLNKNQIYVATLSFINSHITILDAIKPHTLIVDEATQ
                     ILEYDLLAVLSNTKRWILIGDENQLPAVVLQNDKSSTDTMIFTCRYKSNELCPLSANI
                     ANEKCYNKDENNCKALKILNQIHLNDFSEPLFTRLKKNAIARDWSECYIMLRYHHRMH
                     EEIARYPNELFYDKKLLSATERQKSVLSNSYHLPKILKDDREYNERVIFISTPISSEL
                     STPTNPYEAKIVIELIKTLRSQFPQYSIGVITPYRSQIALIKSLLDKTINDITIDTVE
                     RYQGSERDIIIYSFAINNLEYLELSQSMNYDHSVDRKLNVSLTRARELLFLVGNAAIL
                     NSHPFVKQIINDLESRGLVINAK"
     gene            6854..8506
                     /locus_tag="PKI65_05030"
     CDS             6854..8506
                     /locus_tag="PKI65_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOB77837.1"
                     /translation="MQNKIKIENLTITNSQSKILVDAINLDIANNKITAIIGESGSGK
                     TLTAHAIMGLIPKSLTIFQNSKIYYYTENQKIDLLKIEKKQLKALRSKKISMIFQNPM
                     SSLHPLIKCGEQIEEVLKTHTNLSKKHRKEAVFDILRQVGFENPKRIFCAYPHQLSGG
                     EQQRVMICMAMITNPEVLIADEPTTALDAQIQMEIIELIKQLKITYRCSVLFISHDLS
                     LVHSFADYIYVMRNGKIEEKGTTDQIFNEPKEQYTQLLIKSKPTLTYMPHRLPTLHQN
                     IGHHFSKTKNNENKTNDVIVLSTNQLNVVHQNNTIWGQKVYNHTLKNISFDVRNKECL
                     GIIGESGSGKTTLLKTILGLIKPTSGEIKYKNENLDLIKYRSLDTLRNIQWVQQNPYT
                     SLPPNMTIAKHFDAILKLHNIGNGADERDNIIKNTLALCQMSENILDRYPHQFSGGQL
                     QRLAIAQALLVNPEIILLDEPVSSLDVSIQAAIINLLNDIRDFRSITYLFITHDIMLA
                     NYFCDTIIVLYKGEIVEKGISKEVINNPKHKYTKILIDACIT"
     gene            complement(8667..10064)
                     /gene="asnS"
                     /locus_tag="PKI65_05035"
     CDS             complement(8667..10064)
                     /gene="asnS"
                     /locus_tag="PKI65_05035"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOB77838.1"
                     /translation="MQRTKIKEILNSDPSIIIDKEYKVQGWVRTRRDSKNISFIALND
                     GSIINHLQIVVDHDKFDEVFLKKITTGSAIGVKGKILPSPAPKQPIELHADMIEIYGE
                     CPADEYPLQKKHHTLEFLREIAHLRPRTNTFGAIFRIRHNLSFGIHKFFNDKGFYYFH
                     TPIITTSDCEGAGEMFRVTTLDLYNLPFTPEGKIDFTQDFFGKEASLTVSGQLEGELG
                     ALALGEIYTFGPTFRAENSNTTRHLAEFWMIEPEMAFYDLKDNMDLAEEFVKYLIKYL
                     LDHNYEDLQFLNERYDKALLDRLNHVLDTPFERISYTDAVEILKNSKQNFEFPVYWGA
                     DLQSEHERYLVEKYYKKPVIIIDYPKEIKAFYMKSNDDGKTVRAMDVLVPRVGEIIGG
                     SQREDDYDKLFKRIEESQVPIEDVWWYLETRKFGSAPHSGFGLGFERFMLFVTGMGNI
                     RDVIPFPRSPKNIEF"
     gene            complement(10206..11150)
                     /locus_tag="PKI65_05040"
     CDS             complement(10206..11150)
                     /locus_tag="PKI65_05040"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOB77839.1"
                     /translation="MIELYVLGSGSSIPNFKRFSPAQYLKIEGKNILIDCGEGTQIQL
                     QRFGLSILNIDYILISHLHGDHFFGLFGLISSMLLLGRIKPLTIYANENLIPILDNVF
                     QDKLSGTYFPYTFVSLSYDGINKLTENNKFEVYSFPLDHSTPTCGFAIKEKLRPRNIS
                     KKFVEQYNPSIEDIKAIKQGADYFDKNTKKLFKNEDITIDPPQPFSYVYCSDSAFKLY
                     DFEEIKRPEYIFIESTFLNDKSDIARSKKHMTATQAAQLANNLNAKNLIMGHFSAEYR
                     DTCLFLLEASKIFPVDHIIIAYDGLKISYDNYKKAKKI"
     gene            complement(11262..14108)
                     /gene="infB"
                     /locus_tag="PKI65_05045"
     CDS             complement(11262..14108)
                     /gene="infB"
                     /locus_tag="PKI65_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOB77840.1"
                     /translation="MNEVKTYRLNQLTRELGVTFAELQELLKTNGIKVNTPNDRVTEE
                     AFQIIKEYYNLDIDKDTNIKKDKDTSIEEDQNTKIEEVKKITEKEIKTDLKTEPLQQD
                     TKSFEFNIKVKGKIDLDEEVDKATKEKPSTTSKQKIKEDKIQPKEQQHTIADTKQKEQ
                     ATEELITKKYTKEELSETESKTKIASEAIKHTPVNEEEPSIIDKKVEKPYLQEINLKV
                     KYKIDLDEVEPKREKSKQNGKNQARPRKKTMKKVAEADTKGGKKWDKNSPDVTTKEKP
                     KQKYYDNKQSDNKLNQKHTTTKKDRPQKKYGDRDFQRRKKREEKVLTDEEITQKIKET
                     LAKAQTKQKSTPTHYKKIKKQRRLSKQVESEEKNLIEVTEFITTNELANIMNVSPTEV
                     IAKCFDLGLITTINSRLDAETIVLVADEFGYEVKFISLDETEEEEYEDDPTKLRPRHP
                     VVTVMGHVDHGKTKLLDFIRKTNVVAGEAGGITQHIGAYEVTLSNGRKITFLDTPGHE
                     AFTAMRARGAKVTDIAIIVIAADEQVMPQTVEAINHARAAGVPMIFAINKIDKPNANP
                     EKIKEQLANMNILVEDWGGDYQYQEISAKQGTNIDKLLEKVLLQADLMDLKANPERPA
                     VGTIIESGLEKGRGYVATVLIHNGTLRVGDPILAGVYYGKVKAMFNERNQPKTEVGPS
                     SPALILGLNGAPQAGDKLIVSDTEKEAKEKALKRQQLFREQSIRAKKHITLDEIGRRI
                     AIGDFKELNLIVKGDVDGSVEALSDSLLKLSNEHVQVNIIHKGVGAITESDILLAAAS
                     NAIIVAFQVRPTPAIRKLAEQEQIQIKSYSIIYDAIEEIQSAIEGMLEPKIEETILGT
                     AEVKQVFSIKKVGNVAGCIVLDGKIDKNARVRLIRNGIVTYDGEILSLKRFQNDVKEV
                     LTGQDCGIGIKNFNDIKVGDLIEAYITTEVEQKF"
     gene            complement(14199..15431)
                     /gene="nusA"
                     /locus_tag="PKI65_05050"
     CDS             complement(14199..15431)
                     /gene="nusA"
                     /locus_tag="PKI65_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOB77841.1"
                     /translation="MATVSLLEAFNEFQDIKGVDKETLMELLGDIFKDILEKKYGDAS
                     NFYFAINPETGDIELWRNRIIVPDGQLEDYNTQIELKDALKIEPDFMIGEEVTEPYGI
                     ENFTRREIQTILQMIKQHISEFENQVTFEKYNDQIGELVIGEVHQVLKNEIIVIDEDD
                     IELILTKDQMIPGDHYKKGDTIRATVYKIENRGGTPYIYLSRTSNDFLARLMEQEVPE
                     ISDGLITIRKIARIPGVRAKVAVESYDDRIDPVGACVGVKGSRIYPVIQELRNENIDI
                     INFSNNPKVFIQRALAPAKISDIQIEDNKAMVVVKPDQISYAIGKNGQNIELASRITG
                     YDIEVYRELVEGEEEDIDIQEFSDVIDQWIIDQLKAIGCDTAKKILSYSVKDLADLAD
                     FEEETVEEIQKIIKVEFE"
     gene            complement(15437..15871)
                     /locus_tag="PKI65_05055"
     CDS             complement(15437..15871)
                     /locus_tag="PKI65_05055"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77842.1"
                     /translation="MIDKEYIEKIVNEVTEGTHIFLTKLVVDTHGRIKIFIDGDSNVT
                     IDDCIKISRYVEKKLSLIEDDFQLDVSSHGIGNPLINPRQYPKHIGHLMQIKTINNEI
                     FRGKLIKYENEKLTLEIKKNKEKKDKIIDLSEIKECKEVLKY"
     gene            complement(15924..15994)
                     /locus_tag="PKI65_05060"
     tRNA            complement(15924..15994)
                     /locus_tag="PKI65_05060"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15960..15962),aa:Gln,seq:ttg)
     gene            16522..19311
                     /locus_tag="PKI65_05065"
     CDS             16522..19311
                     /locus_tag="PKI65_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOB77843.1"
                     /translation="MQYHLIFNLRYIIITVLFLFSNLAITQNISGYILDDSTKEPIPF
                     VNIRINHSNKGSTTDFNGRFLIDSCNEKDTFCISAIGYQELILKISDIPDNRRNNFII
                     YLEKKDKILETIEITSKKIDNNQIALLNSIKDNTIITTGVSQEIIAKYKEASCTDIVK
                     RLPGISMQDQFIIVRGLSARYNNIYLNDVRAPSIESDQRSFSFDLIPAQFIDYIHIYK
                     SPFSDFSSLFGGALLKIKLREEIDKNSFTFSLGQSINMFTTFKDFQYYGEKGSLLFPI
                     KNIFSLPGSFPTSLANSTNENRLQLDKMLNRLWIPTNATAPTNTNLSMAYSFHKKIKK
                     VDLYSNFMLYSNSNFTTTTFERAGYNHYNTFLDKPDTNYHFRDTSYESRYLNSALANI
                     STLYNNNSFNLVLFVTNITIDKITWREGKDYYGGTNIRGNEMLYKKRNIFLGELSGKH
                     KLRKNTDLQWFGGITTTSMLQPDNKRLTWIRNDQQDNDRYGQYALNFMFTANPAMSGR
                     LFQNLNENEYSAGAKLTQKININEEDGNINFGVFTSNLTRSFEARNFGYRISNVMQFD
                     WDIAYLPTEQIFTDDYINTSNGIILDETTNPSDSYTGNETNYAVYIAWQQKIYKFSWY
                     LGLRAEYNTMRLNSHTTDATNPPIDDDKVNYENSMLFFSPSLAIGYDINEKMKIKFNY
                     GKTYDKPQFREIAPYAFYDYEAKAVIIGNPDLYNATINHFNLRYEYYFSLDELFSFDI
                     FYKTFHNAIEYKIIPAGTGLQYSYQNVPAATAIGLETEIHKKILPCMFVIANVALIKS
                     NVMFKNENIENDRMLQGQSPYVVNGGLFFQFDSLDLQASILYNVFGKRIAFVGDPYTN
                     NPDIYELPYHNLKFNISKTFAKKFMIDFQINNILGKKKEFIQIVKSNTGKDVQMTTEK
                     SIIPRIYSFKFTYKI"
     gene            19734..20642
                     /locus_tag="PKI65_05070"
     CDS             19734..20642
                     /locus_tag="PKI65_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOB77844.1"
                     /translation="MKKSLFIISIVLMINAYNYAQNLGISADGSMPDNSAILDIKSTT
                     GGLLIPRMTTTERNAISNPAQSLIIYNLTTKCVEIYESNQWQTIWCNDPCSGVSTISY
                     DGVTYNIVKIGSQCWFKENLRTTKYNDNNSIPEVTDASIWTSTTSGAYCCYDNNPSNC
                     DTYGALYNWYAVNTGKLCPSGWHVPTDDDFKTLEMYLGMTQAQADTIEWRGTDQGSKL
                     AGNAGLWTDGALVQNPNFGTSGFSALPGGYRYSTFYSIGNYSCLWTNTEFGISAWYRK
                     IEPDKIKIFRNSHNKEGGFSVRCIRD"
     gene            21033..>22200
                     /locus_tag="PKI65_05075"
     CDS             21033..>22200
                     /locus_tag="PKI65_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77845.1"
                     /translation="MKKTSIILIFIAMLMPLIGYSQSSINDSFFDQVSYRGAFDDQND
                     WTTGLANWDPQNTNYPATTQTISGEITSNTMWTSDKVYLLQGFVYVVNGVTLTIQPGT
                     IIRGDKNSKGTLIIERGAKLIAQGTASEPIVFTSNQPAGSRNYGDWGGVILCGSGKIN
                     VPGGEAQIEGGPRSYYGGTNDNDNSGILNYVRIEFPGIPFQPDKEINGLTMGGVGKNT
                     TIDYVQVSYSGDDAYEWFGGCVNAKHLITLGTWDDDFDTDYGFSGMVQYAVALREPNI
                     ADVSGSNAFESDNDASGSNNIPQTSAVFSNVSILGPKKDASIIINNNYKRAMHLRRNT
                     GIDIYNSVFIGWYDGLLIDGSACESNANNSILNIKYVTMAAMAGNYFTAASESTF"
BASE COUNT         7629 a         3311 c         3196 g         8064 t
ORIGIN      
        1 acaccagtaa ataccttgct atgagtaagg aagagaataa agaattctta gacaaccttt
       61 attctctaat gaaaaaatta gaagctgatg tagtcgatta tcaaggtctg aaaatactca
      121 gtgccccacc actatgccca gactgtcgta gcaatcacat cattaaaaat ggtttacaaa
      181 atgggatgca aaactatcgc tgcaaagact gtggcagaca gtttagagtt accacaggta
      241 cttttgttta caggttgcaa aagaaagaga aaatgctcga ctacatcaga tgtatggtag
      301 caggcaaaag tctcagagct tgtgctaaag aagtgggcat cagcttacca acaagcttcg
      361 cttggcgaca caagatatta gcagctttac aaagtttcga agataatatc aattttttcg
      421 gtgtagtaga gatggacgaa ctattgatgg attattccga aaaaggcaga atatacaaaa
      481 atgccgaaga gctgaagcga gctaggaagc aaaagccaaa gaaagtagcc gtattggctg
      541 ctacagacag agctggcaat ttattattca aaaaatttga aggcaaaaag ctaagttcag
      601 aacaaataga gcaatttctg aaggctaaga tgccaaagga tggagtcatg tgtagcagta
      661 ataaaaaagc attcaaaaag gttagaaaaa atgtaatcaa gcacaaagaa atagtaacta
      721 gcaataggca aaagcgaggt atatatagcc taagtactgt caagaaaaag gctagtgaat
      781 tcactaattg gatatatttc aaatttaggg gcgtagctac caaatatctg caaaactaca
      841 ttatgtggta tgtagtgatg ggtaaatacc tcacaggcaa gactgtaagc aatactgggc
      901 gaatgctcaa cctcgcatcc tcggacagat gggcgtggta tcggtataga gagttagtaa
      961 aattaacata tgttaattga aacatatatt agttttcaat ttcttttcgc ttcttaaaaa
     1021 atattaaata cccctcataa ataataaaat tattaaagat ctagaacctt atgattgaga
     1081 aataactccc aaatatttta atgtatttat tgataataca aaatcaaata cttgctaaca
     1141 ttgattataa ctaaaatttg aatgagtttt atatttattt tctttctatg tctgcaccta
     1201 atgcttgaag gcgcttatct atattttcat agcccctttc tatttgttct atattatgaa
     1261 ttatacttgt gcctttggca gacaatgcag ctattaatag ggatataccc gctctgatat
     1321 caggactaga cattgtaatt gctctcagtg gatatcgtcg ttcgagtcct atgacagtag
     1381 ctctatgtgg atcacacaat attacttgag cacccatttc tattagatta tctacaaaaa
     1441 acaatctact ttcaaacatt ttttgatgta tcaatacaga tcccttagcc tgtgtagctg
     1501 ctactagaac tatacttaat aaatcaggtg taaagcctgg ccatggggca tctgatatgc
     1561 taagtatgcc accatcgata tattgtgaaa cctcatatat atcttgatgt ggtactatca
     1621 gattatcatc gtcttctatg attattatgc ctaattttcg aaaggtatct gggattattc
     1681 ctaagtgtgg tatattacaa tttttaattg ttatattaga ctgtgtaaga gcagctaaac
     1741 ctatgaaact accaacatct atcatatctg ctaaaagttt atgagtagta ccatgaagtt
     1801 tttgtacacc ataaattttt aaaagattag atcccacgcc ctcgatgtat gctcccatag
     1861 aaattaacat tttgcaaagt tgctgtatgt aaggttcaca agcagcgttg tagatggtag
     1921 tagtaccttc ggctaataca gctgccatta ttatattagc tgttccagtt acagaagcct
     1981 ctggcagcat tatgtagttt cctttcaatc cgttgttagc tgtcagtgtt acttgatttg
     2041 taaaataatc aaatttgatc tctgcaccca atttttgaaa tccgaaaagg tgagtatcta
     2101 tacgtcgcct gcctatttta tctccaccta ttttaggtat tatagctttt ttgtattttg
     2161 tcaatatagg gccagctaac attaatgatc ctctcaaaag agattgtttt tcttttataa
     2221 actcctcttc taacatatcc aaatttatat tacgagcaca tatagttaca gagttattag
     2281 ctctatctat agctacttct atacctaaat catgtattag attgaggaga ttcattatat
     2341 ctcttgtgag tggtatattt aataatttta caggttcatc tgttaatagt gctgcacata
     2401 ttacttgtaa ggcttcattt tttgctcctt gtggttctac aatacctgat agttgtttgc
     2461 caccttttac tatgaaatca gccattttat ttatcttctt tttttatttt tgttataata
     2521 ttttttactt ttttgacttt cgtagctcga tttaataatg tttacattag tagtatcaaa
     2581 gattttttca ggtattttaa gtttaccttg agagagtgta tttatatgat tttttattat
     2641 atcatctgtt accattcgat tattccaata catatataat tttttcatag tcataattag
     2701 atgtcttaaa agttcatttt tttcgtctcc atcaggcatt tctatagctt tatttatcat
     2761 ctcttctatt attctaccat aatagcgaaa tttagaatta tattcttctt tatatgaaat
     2821 gcgttcaggt acgaaatgca aatatttgat atttacttct accggccaat ctatatcaag
     2881 ctctccatca gataatatgt ataaatgatc ccaatatttt tgattattat atttaccatt
     2941 gcctactata gaagccatag ttttcactat cttgtgagca aattgtgtac gctcttcttt
     3001 gtctttaatt gtttttgcat aatctaccat cttttgtata ttacgaccat attcggatat
     3061 cttcaaaggt tccaattctg aattatattt catatatatt ttttattaat ttacaaatta
     3121 taactgaaaa ataacaaatt gtttttttta aaaaataaaa aaatttagaa ttttattttc
     3181 aatttaaaaa aattaagttt aatgattaat atgtaatctt ttattaaaac aattttgcaa
     3241 taattgttat tgcaaagtta ataattattt ttgaaatgtt tagaaaatta aaaaaaacaa
     3301 ctaattttat taataaattt tttattaatt ttatttttat aaaaaacaat tataatggat
     3361 aactacaaat ctattgctca actaatagat gatattgaaa aaaatatttt aaaaattaat
     3421 aatttatcac ataataataa atttactaaa atagatgatt tgatagggtc attgaatgat
     3481 actttcttat cctatcctat cctcatagat gcttttcatc aatttaatgt gctaaaaaac
     3541 aaattcacac aagtaagaac tctagatcat aatgatttat taaagttaaa aaatttattt
     3601 gaaataatca tagataattt gaaaatcaat actacaaaac aaaattattt atatacattt
     3661 agaaatcaga tcattgatat atctatacct caaattgtac gtaaacctta ttttaattat
     3721 caaaatttat gtcatctaag aggcatcatc gaagatatca aaactgagtc tgatcgacta
     3781 atcataactc tatatactat aagtgctgat gggccatttt tagatgagat cagagtgcat
     3841 ttatttgatc atagtaaaaa taatctttta tacccagatc ttactcagta cggatctata
     3901 ctagagactg gacaagatat ttttatacaa aattgtactt atttaaaaga aaaagaatat
     3961 tattctacag ctgacacact gatggtacta tatccagact atattttgga tgttagagat
     4021 gtgagtgatg tacatatttt tgacaattca tatcctcatt tcatcagact catacctatc
     4081 tattcgatta ttagaacatt tgtaaatata aataatgttc aaaaaattca aagcatctta
     4141 ataggcaatc ttttaggtga aatttttgat acatatgtag cttatgatag agtagaagaa
     4201 gcacatttat tgacagcata taaagatttt attggaaaaa atatctttga gttattatat
     4261 tggtataata ataatacaga agggtttaat cttagttttt tgatacaaat tatagaagat
     4321 gaatatttac ctggtctaca gttaatgcta ccaagtataa aaagagataa aagcatgatt
     4381 ttatatttag aaccatttta tattagcaat ttatacggtt tacaagggag aatggatgca
     4441 ttagcattta ctgaaaaagg caatagtgct aatatttatg aattaaaatc tcataaaccc
     4501 aaaaacaaag gttatttata taccaaatcg catgatgcac agttgatatg ctattatctt
     4561 ttatttcgta gcacatatcc cgaaaaaaaa atattttata aaatggcgta tcctggcgcc
     4621 gaggagcgtc atctacgaag tatcactttt ggcgaaaatg ccgatgaaag ggaactaatg
     4681 ttgatacagg aagtactttt tgctcgtaat aatattgtaa aagcacactt agattatgcc
     4741 aaaggcgact tctcttctct aattcgatgt atgcctaaaa aatctgatac tccttctccc
     4801 aatactattt ccatttttag caatacagaa actaaattga gagattcaca aacttttatg
     4861 tataatgata ttattatcat gcataatacg ctaaaagcta ttgccgataa taaattatta
     4921 aatgcatatt tttggggtag tgtgcgcttt atttatcgcg aaatgctcag tgctaagact
     4981 ggaaatccag aaacaacaga tatatgggaa agcatgataa aagaatctaa atggggttat
     5041 gctaatattt ggaaaaaatc tttaaacaaa aaatttaatc aatatgaggt actaggaccc
     5101 ctaatattta aatcttttac accagacaat catttgattt ttagtttaga taatgatgat
     5161 ttatctattt ctcgactccg agaagatgat attatcatat tatatcctta tgatgaaaat
     5221 aaaaatagcc ctctctcaca gcaattactg aaaggttata taatagatat aaatgagagc
     5281 aaggttaagc taagtcttgc aaataaattt atacctaaaa aaatatttaa agccaaatct
     5341 aaatggttag cagagtctga tagatggttt cataactaca gttatggtat tcgtaattta
     5401 ttttatctaa tttctaatga taataaaaaa tttattaatt tatttcttgg tattgataga
     5461 cctgagtttg ttgatgttaa tcaatcaata gtaggattag aaactctaga tgattctcag
     5521 gcaaaagcca ttaaacaatc cttgagtgct aatgattatt ttttgattca aggccctcca
     5581 ggtactggta aaacagctaa aacattgact actattgcca agaatttgta taataataat
     5641 gtaaaaacac ttatagtagc ttatactcgt agagctgtag atgaaatatg taataattta
     5701 gacaaacaaa gcatacctta tttattttta aataaagatt tagtaaaaaa tcaattaaat
     5761 acggctaata atctaaatga tttggataat atattgcctg atttaaataa taatttaaat
     5821 aaaaatcaaa tttatgtggc cactctttcc tttattaatt ctcatattac tatcttagat
     5881 gctataaaac cacatactct gatagtagac gaagctactc aaatactaga gtatgattta
     5941 ctagctgtat tatccaatac aaagagatgg atactcatag gagatgaaaa tcaattgcca
     6001 gcagtagttt tacaaaatga taaatcatca acagatacta tgatattcac atgtagatat
     6061 aaatctaacg aactctgccc attatctgca aatattgcta atgaaaaatg ttataataaa
     6121 gacgaaaata attgtaaagc tctcaagata ttaaatcaaa ttcatttaaa tgattttagc
     6181 gaaccacttt ttactagatt aaagaaaaat gctattgcca gagattggtc agagtgctat
     6241 atcatgctgc gatatcatca taggatgcat gaggagatag ctcgatatcc taacgaatta
     6301 ttttatgata aaaagttatt aagtgctaca gagagacaga agtcagtcct gtctaattca
     6361 tatcatttac ctaaaatatt aaaagatgat cgagagtata atgaaagggt aatatttata
     6421 tctactccaa tatcttcaga gttatctacg ccaacaaatc cttatgaagc taaaatagta
     6481 atagaactta ttaaaaccct ccgaagtcaa tttcctcaat attctatagg tgtcattact
     6541 ccatatcgtt ctcaaatagc attaataaaa tcgcttttag ataaaactat taatgatata
     6601 acgatagata cggtggagcg atatcaagga tcagagagag acattatcat ttattctttt
     6661 gcaattaaca atttagagta tttagaatta tcacaatcaa tgaactatga ccatagtgta
     6721 gataggaagc taaatgtttc attaacaaga gcacgtgaat tattattttt agtaggtaat
     6781 gcagcaatat taaatagcca tccatttgtt aaacaaatta ttaatgattt agagagtcga
     6841 ggtttagtaa ttaatgcaaa ataaaatcaa aattgaaaat cttacaatta caaattcgca
     6901 aagtaaaatt ttagtagatg ctattaattt agatatagca aataataaaa ttacagctat
     6961 tataggtgaa agtggtagtg gaaaaacatt gacagcacac gctattatgg gtttgattcc
     7021 aaaatcctta actatctttc aaaatagtaa aatatattat tatacagaaa atcaaaaaat
     7081 agatttatta aaaattgaga aaaaacaatt gaaagctttg agaagtaaaa aaataagtat
     7141 gatttttcaa aatcctatgt cttccttgca tccattgatt aaatgtggtg aacaaatcga
     7201 agaagtgtta aaaacccata ctaatttatc taaaaaacat agaaaagaag cagtttttga
     7261 cattttaaga caagttgggt tcgaaaatcc taaaagaatc ttttgtgcat atccgcatca
     7321 gctttctggt ggagagcagc aacgtgtgat gatctgtatg gctatgatta caaacccaga
     7381 ggtgttaata gctgacgagc caactacagc actagatgca caaatacaaa tggaaataat
     7441 agaattaatt aagcaattaa aaataacgta tagatgttca gtactgttta tttctcatga
     7501 tcttagttta gtgcactcat ttgctgatta tatttatgta atgagaaatg gcaaaataga
     7561 ggaaaaggga actactgacc aaatatttaa tgaaccaaaa gagcaatata ctcagttact
     7621 tataaaatcc aaacctaccc ttacttatat gcctcataga ttacctacat tgcatcaaaa
     7681 tatcggtcat catttctcaa aaacaaaaaa taatgaaaat aaaactaatg atgtaatagt
     7741 attatctaca aatcaactaa atgtcgtaca tcaaaataat actatatggg ggcaaaaagt
     7801 atataatcac acattaaaaa atatcagctt tgatgttaga aataaagaat gtctaggaat
     7861 aataggagaa tctggtagtg gtaaaacaac attattaaaa acaattctag gattaataaa
     7921 acctacaagt ggagaaataa aatataaaaa tgaaaattta gatttaataa aatatcgttc
     7981 actagacaca ttgagaaata tacagtgggt gcagcaaaat ccttacacct cccttccacc
     8041 taatatgact attgctaagc atttcgatgc tatattaaaa ttacataata taggtaatgg
     8101 tgctgatgaa cgtgataata ttattaaaaa tactctagcc ctttgtcaga tgtcagaaaa
     8161 catcttagat cgttatccac atcaatttag tggtggtcaa ttgcaaagat tagctatagc
     8221 acaagccctt ttagtaaatc ctgaaataat tttattggat gaaccagtct catcattaga
     8281 tgtatctatt caagcagcaa ttattaattt attaaatgac attagagact ttagatctat
     8341 tacgtattta tttataactc acgatatcat gcttgccaat tacttttgtg atactataat
     8401 agttttgtac aaaggtgaaa ttgtagaaaa aggaatatca aaagaagtaa taaataatcc
     8461 taagcataag tatactaaaa tattaataga tgcatgtata acttaaaaat agtttcatct
     8521 ttatcaatag atattaatat caaaattaat caaaaattaa aatataataa aacaactcac
     8581 tcccttctcc ttgcctaagg ctattactat tattttagga taaaaattgg ttagtacgat
     8641 aaaatatatt tattgttaat tgcaaattaa aattcaatat tttttggtga tcttgggaaa
     8701 ggtatcacat ctctaatatt ccccattccc gttacaaaca acataaacct ttcaaatcct
     8761 agtccaaagc cactatgagg ggctgaacca aattttcttg tttctaaata ccaccataca
     8821 tcttctattg gtacttggct ctcttcgatt cgtttaaata atttatcata atcatcttct
     8881 cgttgactac cacctattat ttctcctact ctaggcacta gcacatccat agctcttact
     8941 gtcttgccat catcatttga tttcatataa aatgctttta tctcttttgg ataatcaatt
     9001 attataactg gttttttata atatttttct actaaatatc tttcgtgttc agattgtaaa
     9061 tctgctcccc agtacacagg aaactcaaaa ttttgctttg aattttttaa tatttctaca
     9121 gcatcagtat atgaaattct ctcaaatgga gtgtctaata catgatttag cctatctaac
     9181 aatgctttat cgtatctttc atttaaaaat tgtaaatctt catagttgtg atctaataaa
     9241 tattttatta aatatttcac aaattcttcg gctaaatcca tattatcttt taaatcataa
     9301 aaagccatct ctggctcaat catccaaaat tcggctaaat gccgagtagt attactattt
     9361 tcagctctaa atgtagggcc aaatgtataa atttcgccta acgcgagagc acctaattca
     9421 ccttctaatt gtccagatac cgtaagtgaa gcctctttac caaaaaaatc ttgagtaaaa
     9481 tctattttac cttcaggagt aaatggtaaa ttatataaat ctaaggtagt aacgcgaaac
     9541 atctcgccag caccttcgca atcacttgta gtgataatag gtgtatgaaa ataataaaaa
     9601 cccttatcat taaaaaattt atgtatacca aaagatagat tgtgccttat tctgaaaata
     9661 gcgccaaaag tattggtcct aggtctcaga tgggctatct ctctcaaaaa ttctaaagta
     9721 tgatgttttt tctgcagtgg atactcatct gccggacact ctccatatat ctctatcata
     9781 tcagcatgta attctatagg ttgcttaggt gctggtgatg gtaatatttt accttttact
     9841 cctatagcac tacctgttgt tatttttttt agaaatacct catcgaattt atcatgatct
     9901 actactatct gtaaatgatt tataatgctt ccatcattta gagctatgaa gcttatattt
     9961 ttgctatcgc gtctagtacg tacccaccct tgcactttgt attccttgtc tattatgatc
    10021 gagggatctg agtttaatat ttctttgatt tttgttcttt gcatttagtt taatttttta
    10081 tgcaaatttc gtaaaaatta tagtaataat aaaaaattta ttagaatcag cattttatta
    10141 actaaccaaa tctaaaataa aattatctat tattaaggta aaaggtaaaa agatagattt
    10201 aagtattaaa tttttttggc ttttttataa ttatcataag atattttcaa cccatcataa
    10261 gctattataa tgtggtcaac tgggaaaatt ttacttgctt ctagtagaaa taaacaagta
    10321 tctctatatt ctgcactgaa atgtcccata attaagtttt ttgcattcag attattagct
    10381 aattgagctg cttgggtagc tgtcatgtgt ttttttgatc tagctatatc agatttatca
    10441 tttagaaatg tagactcgat gaatatatat tctggtcttt tgatttcttc gaaatcatat
    10501 agtttaaaag ctgaatcact acagtaaaca taagaaaagg gttgtggtgg atctatggta
    10561 atatcttcgt ttttaaataa ttttttagta tttttatcga aataatctgc cccttgcttt
    10621 atagctttta tgtcttcaat actaggatta tattgttcta caaatttttt tgatatatta
    10681 cgaggtctta gtttttcttt aatagcaaat ccacaagtgg gagttgaatg atctaaggga
    10741 aatgaatata cttcaaattt attattttct gtcaatttat ttataccatc ataagatagt
    10801 gatacgaagg tatatgggaa ataagtacct gataatttat cttgaaaaac attatctaat
    10861 attggaatta aattttcatt agcatatatt gtcaatggtt ttattctacc tagtaataac
    10921 atagaagaga tgagcccaaa taacccaaaa aaatgatctc catgcaaatg agaaatcaga
    10981 atgtagtcaa tatttaatat agatagacca aatctttgta attggatttg agtaccctca
    11041 ccacaatcta ttaatatatt tttgccttct atttttagat attgagccgg actaaatctc
    11101 ttaaaattcg gaatagaaga tccactgcct aagacataga gttcaatcat tttttattta
    11161 tttttataag ttttcaattt tcattgaaag gtttgcaaat acaagtttta acaattattt
    11221 taaatatttt gataaaattg ttattagaaa aagtgtatat attaaaattt ttgttctact
    11281 tctgtcgtga tatatgcttc tatgaggtct cccactttaa tatcattaaa atttttaata
    11341 ccgataccac aatcttgtcc tgtaagtact tcttttacat cattttgaaa acgtttgaga
    11401 gataaaattt caccatcgta ggttacgata ccattacgta tcaaccttac acgagcattt
    11461 ttatcaattt taccatctaa aactatacaa ccagctacat taccaacctt tttaatagaa
    11521 aatacttgtt ttacctcagc agtaccgagg atagtttctt ctattttagg ttctagcata
    11581 ccttcaatag cagattgtat ttcttcgata gcatcataaa taatagaata ggattttatt
    11641 tgtatttgtt cttgttcagc taatttacga atggcaggtg tagggcgaac ttgaaaggct
    11701 acaatgatag cattagaggc tgcagctaat aaaatatcag attctgtaat agcaccgaca
    11761 cctttatgaa taatatttac ttgcacatgt tcattagata actttaataa tgaatcactt
    11821 aatgcctcta ctgacccatc aacatcacct ttgactatta aattgagttc tttgaaatct
    11881 cctattgcta ttctacgacc aatctcatca agagtaatat gttttttagc tctaatagat
    11941 tgttctctaa aaagttgttg acgtttaaga gctttttctt tagcttcttt ttcagtatcc
    12001 gacactatca atttatctcc agcttgaggt gcaccattaa gaccaaggat tagagccggt
    12061 gagcttggtc ccacttcagt ttttggttga tttctctcat taaacatagc ttttacttta
    12121 ccataataaa cacctgctaa tataggatca cctacacgca aggtgccatt atgaatcaaa
    12181 acagtagcta cgtaacctct acctttttct aatccagatt ctataatagt acccacagct
    12241 ggtctttctg gattagcttt tagatccatc aagtcggcct gtaacaaaac tttttctaat
    12301 aatttatcta tatttgttcc ttgtttagct gatatttctt gatattgata gtctcctccc
    12361 caatcttcta caagaatatt catattagca agctgctctt ttattttctc ggggttagca
    12421 tttggtttat caattttatt aattgcaaaa atcatgggta ctccagcggc acgagcatga
    12481 ttgatagctt caacggtttg aggcatcact tgttcatcgg cagctattac aattatagca
    12541 atatcggtta ctttagctcc tcttgcacgc atagcagtaa aagcttcgtg tccaggagta
    12601 tctaaaaaag taattttgcg tccattagat aaagttactt cataagcacc aatatgttgc
    12661 gtgataccac cagcctcacc tgctaccaca ttggttttgc gaataaaatc aagtaattta
    12721 gttttaccat gatctacgtg tcccattact gtaactacag gatgacgtgg tcttaatttt
    12781 gttggatcat cttcatattc ttcttcctca gtctcatcta aacttataaa tttgacttca
    12841 taaccaaatt catcagctac taacactatt gtttctgcat ctaatcttga attaatagta
    12901 gtaatcaacc ctaaatcaaa acatttggct attacttcag taggcgatac attcattatg
    12961 tttgctaatt catttgtagt gataaattca gttacttcaa tgagattttt ttcttcactc
    13021 tctacttgtt ttgatagtct tcgttgtttt ttaatttttt tataatgtgt aggagtactc
    13081 ttttgcttag tttgagcttt tgctaaagtt tctttgattt tttgtgtaat ttcttcatct
    13141 gttaatactt tttcttctct tttttttcta cgttgaaaat ccctatcacc atattttttt
    13201 tgaggtctat ctttcttagt agttgtatgt ttttgattta atttattatc agattgttta
    13261 ttatcataat atttttgttt tggtttttct tttgtagtta catctggtga atttttatcc
    13321 cattttttgc caccttttgt atcagcttcg gcaacttttt tcatagtttt ttttctaggt
    13381 ctggcttgat ttttgccgtt ttgcttgctt ttttctcttt taggttctac ttcatccaga
    13441 tctattttat acttaacctt tagattgatt tcttgtaaat aaggtttttc tactttttta
    13501 tcgattatgg atggttcttc ttcatttaca ggagtatgct ttattgcttc acttgcaatt
    13561 tttgtcttag attctgtttc cgaaagttct tcctttgtgt atttttttgt tattaattct
    13621 tcagttgctt gttctttttg ttttgtatca gctatagtgt gttgttgttc ttttggttgt
    13681 attttatctt cttttatttt ttgttttgat gtagtactgg gtttttcttt tgtagctttg
    13741 tctacttctt catctaaatc tattttacct tttactttaa tattaaattc aaaagattta
    13801 gtatcttgtt gtaaaggttc tgtttttaaa tcagttttta tctctttttc agtaatcttt
    13861 tttacttctt caatttttgt attttgatct tcttcaatgc ttgtatcttt atctttttta
    13921 atgtttgtat ctttatctat gtcaaggtta tagtattctt ttattatttg aaaagcttcc
    13981 tcagttacac ggtcattagg agtatttact tttattccat ttgttttgag taactcttgt
    14041 agctcagcaa atgtaacacc taattcacgt gtaagttgat ttaatctata tgttttaact
    14101 tcattcatat tttaaaatct ctcaatgcaa aattaactta tattttttaa acactttttt
    14161 atatttattt ttaattaaat ttataaatca ttacaaaatc attcaaattc aactttaata
    14221 attttttgaa tttcttctac agtttcttct tcgaaatctg cgaggtctgc tagatctttt
    14281 acagaataag atagtatttt tttagcagta tcacaaccga tagcttttaa ttgatctatg
    14341 atccattgat cgataacgtc gctaaactct tgaatatcaa tatcttcttc ttcgccttct
    14401 actaattcgc gataaacttc gatatcataa ccagtaatgc gcgaggctag ttcaatattt
    14461 tgtccatttt tgccgatagc ataagatatt tgatctggct tcaccactac catagctttg
    14521 ttatcttcta tttgaatatc agaaatttta gcaggtgcta gagctctttg tataaaaact
    14581 tttggattat ttgaaaagtt aattatatca atattttcat ttcttaattc ttggataaca
    14641 gggtaaatac gagatccttt tacacctacg catgcaccta caggatcgat acggtcatca
    14701 taagattcta cagctacttt agctcttaca ccagggatac gtgctatctt acgaatagtt
    14761 attaggccat cagatatctc tggtacttct tgttccatta gcctggctaa aaaatcatta
    14821 ctcgtacgag acaaataaat atatggagtt ccccctctat tttctatttt gtaaactgta
    14881 gctctgatag tatcgccttt tttgtaatgg tcgccaggta tcatttgatc ttttgttaat
    14941 attagttcta tatcatcctc atctattact ataatttcat tttttagcac ttgatgtact
    15001 tctcctatta ctaattcgcc aatttgatca ttatattttt caaaagttac ttggttttca
    15061 aattctgaaa tgtgttgttt gatcatttgc aaaattgtct gtatttctct acgagtaaaa
    15121 ttttcgattc catatggttc tgtaacctct tcacctatca taaaatcagg ttctatcttg
    15181 agagcatctt ttaattctat ttgtgtatta taatcttcta attgtccatc tggcacgatg
    15241 attctattac gccataattc tatatctcca gtttcaggat ttatagcgaa ataaaaatta
    15301 gatgcgtctc catatttttt ttccaaaata tctttaaaaa tatctcctaa taattccatc
    15361 aaagtttctt tgtctacacc tttaatatct tgaaattcat taaaagcttc caataaactt
    15421 actgttgcca taaattttaa tattttaaaa cttctttaca ttcttttatt tctgataaat
    15481 ctattatttt atcttttttt tctttatttt ttttaatttc taatgttaat ttttcatttt
    15541 catattttat gagtttccct ctaaagattt cattatttat agtttttatt tgcatcaaat
    15601 gtcctatatg tttaggatac tgacgaggat tgattagtgg attaccaata ccatgcgaag
    15661 atacatctag ttgaaaatca tcttctatga gtgagagctt tttttctaca tatctactta
    15721 tttttataca atcatctatg gttacattac tatctccatc tataaatatc tttattctac
    15781 cgtgagtatc tacaactaac ttggtaagaa agatatgtgt cccctcagtt acttcattta
    15841 caattttttc aatatattct ttatctatca tctttttata ttacaattag agggagctaa
    15901 ttgccccctc tcttatatgc ctactgtccg accaggattc gaacctggac aggcagaacc
    15961 aaaatctgca gtgctgccgt tacaccatcg gacaatttat tttgcaaaat tacaaaattt
    16021 tttttatttg aaaaaataaa ttttataatt taattaatag attataactg atagatttaa
    16081 tttattataa ttatctagta ttataataaa atttcgaaaa gtcattttta tattaaatca
    16141 attttttgtt aaaataaatc aataaataat gcagataata tgcatttaaa cttaggtata
    16201 tttaattgta atgtgtaata gttattatga gtaaaaataa agtatatgtg cttttgctca
    16261 agtgaaacag tatttttctg tagatatcta ttaaaaaatg ttttcagagc ttaagcaaaa
    16321 attatctata aattaaatag ctcaacgtga aagtagcaca atctccctta tttttattga
    16381 ttttgttttt tattacattt ttaaaatttt catcacatat tagtgaagta ataaattaac
    16441 acattcttaa tattaattaa caaataaaaa atagtaattt aaaataaaga ttactttgag
    16501 aatatacttt tgcaaaaaaa tatgcaatat catttaattt ttaaccttcg gtatattatt
    16561 attactgttt tatttttatt tagcaatcta gctataactc agaacatcag cggatatatc
    16621 ctagatgatt ccactaaaga gcctatacct tttgtaaata tcagaattaa tcatagtaat
    16681 aaaggtagta caactgattt taatggaaga tttttaattg atagttgtaa tgaaaaagat
    16741 acattttgca tttcggctat tggttatcaa gaattaattt taaaaatatc agacatacca
    16801 gataatcgta gaaataattt cattatttat ttagaaaaga aagataaaat cttagaaaca
    16861 atagaaatta ctagtaaaaa aattgataat aaccagatag cattgttaaa cagcataaaa
    16921 gataacacta ttattactac aggagtttct caagagataa tagctaaata caaagaagca
    16981 tcgtgcacag acattgttaa acgtttgcct ggtataagta tgcaagatca atttatcata
    17041 gtaagaggtc tgagtgcaag atataacaat atttatttaa atgacgtgag agctccaagt
    17101 attgaatcag atcagagaag tttctctttt gatcttatac ccgctcaatt cattgattac
    17161 attcatattt acaaatctcc tttttcagac tttagctctt tatttggtgg tgctttatta
    17221 aaaataaagt taagggaaga aattgacaaa aattctttta ccttttctct ggggcagtct
    17281 ataaatatgt ttacaacatt taaagatttt caatattatg gagaaaaagg gtcactttta
    17341 tttccaataa aaaatatttt ttcacttccc ggttcatttc ctacttctct agctaatagt
    17401 acaaatgaaa ataggctaca attagataaa atgttgaata gattatggat tcctactaat
    17461 gctacagctc ccactaatac aaatctatct atggcatatt catttcataa aaagattaaa
    17521 aaagtcgatt tatatagtaa ctttatgctt tatagtaatt ctaattttac aacaactacc
    17581 tttgagaggg ctggttataa tcattacaat actttcttag ataaaccaga tactaattat
    17641 cattttcgag atactagtta tgaatcaaga tatttaaata gtgctttagc taatataagt
    17701 acattatata ataataatag ttttaatttg gttttatttg ttactaatat tactatagat
    17761 aaaataactt ggagagaggg taaagattat tatggtggta ctaatattcg tggtaatgaa
    17821 atgttgtata aaaaaagaaa tattttttta ggtgaattgt ccggtaaaca caaattgaga
    17881 aaaaacactg atttacagtg gtttggaggt atcacaacta catctatgct gcagccagat
    17941 aataaacgac ttacttggat tagaaatgat caacaagaca atgatagata tgggcaatat
    18001 gctttgaatt ttatgtttac agctaatcct gctatgtctg gaagattatt tcaaaattta
    18061 aacgaaaatg aatatagtgc aggagctaaa ttaactcaga aaatcaatat taatgaggag
    18121 gatggtaata taaattttgg cgtatttact agtaatctta cgagatcttt cgaggctaga
    18181 aactttggtt atagaatatc taatgtaatg cagttcgatt gggatattgc ttatttacct
    18241 actgaacaaa tatttacaga tgattatata aatacatcga atggaattat attagatgaa
    18301 actactaatc ccagtgattc atataccggt aatgaaacta attatgctgt ttatatagct
    18361 tggcagcaaa aaatatataa attttcatgg tatttgggtc tgagagcaga atataacact
    18421 atgagactaa attcacacac tacagatgct actaatcccc ctatagatga tgataaagta
    18481 aactatgaaa attcaatgtt attttttagt ccttcattag caataggtta tgatattaat
    18541 gaaaaaatga aaattaaatt taactatggt aaaacttatg ataaaccgca gttcagagaa
    18601 atagctccat atgcattcta tgattatgaa gctaaagccg tgataatcgg caatccagat
    18661 ttatacaatg ctacaataaa ccattttaat ctacgctatg aatattattt ttctttagat
    18721 gaactttttt cttttgatat tttttataaa acttttcata atgctattga gtataaaata
    18781 ataccggccg gcacaggttt acaatattct tatcaaaatg tacctgcagc tactgctata
    18841 gggttagaaa ctgaaattca caaaaaaata ttaccatgca tgtttgttat tgctaatgta
    18901 gctttgataa aaagtaatgt aatgtttaaa aatgaaaata ttgagaatga tagaatgcta
    18961 caaggtcaat ctccatatgt agttaatgga ggtctatttt tccaatttga ttctttagat
    19021 ctacaagcta gcatattata taatgttttt ggcaaacgta tagcatttgt cggagatcca
    19081 tatactaata atccagatat ttatgaatta ccctatcata atcttaaatt taatatttct
    19141 aaaacttttg ctaaaaaatt tatgattgat tttcaaatta ataatatttt aggtaaaaaa
    19201 aaagaattta tccaaattgt aaaatcaaat actggcaaag atgtgcaaat gaccacagaa
    19261 aagagtatta ttcctagaat atatagcttt aaatttactt acaaaatatg attataccat
    19321 actaaaggca acattcatgt ggccgtataa attaatctta actgcagata ttcagatgat
    19381 agtaggaatg tacaaaaaat aaggagatgg aataataaag aaatataaaa tttatacgtg
    19441 tagaaataga tgagtaaaaa agataaaatc aacagaaatg ttatccgatc aaaaaaataa
    19501 ttatagccct gtaaagcaaa tctaaaacct tttttaggac aaataccctt tagattttaa
    19561 attaaacaaa tattatttca actgtatata attttttaaa ttgttgataa tcaatatgaa
    19621 atttgagaaa tatattcatt cacaatgtgt atgattttta tcttatgggt atttcatata
    19681 aaaaattata tatttttgta aacacgaatt taaaataaaa caatataaaa acaatgaaaa
    19741 aatcattatt tatcattagt attgtgttga tgataaatgc atataattat gcacaaaatt
    19801 tgggtatcag tgcagatggt agcatgcccg ataattcagc tattttagac atcaaatcta
    19861 ctactggcgg cttactaata ccacgtatga ccactaccga gcgcaatgct atctctaatc
    19921 ctgcacaatc tctaataatt tataacctta ctaccaaatg tgttgaaata tatgaaagca
    19981 accaatggca gactatctgg tgcaatgatc catgtagtgg agtatctaca attagttatg
    20041 atggggtaac atacaatatt gtaaagatag gcagtcaatg ttggtttaag gaaaatctga
    20101 ggacaacaaa atacaatgat aataattcaa taccagaggt aacagatgct tctatatgga
    20161 catctaccac ttcaggtgcc tattgttgtt atgataataa tccatctaat tgtgatactt
    20221 atggtgcttt atacaattgg tatgcagtaa atacaggtaa attgtgccca agtgggtggc
    20281 atgtaccaac agatgatgat tttaaaacat tagagatgta tttaggaatg acacaagcac
    20341 aagcagatac gatagaatgg agaggtacag atcaagggtc taagttagct gggaatgcag
    20401 gtttatggac agatggtgct ttagtacaga acccaaattt tggaactagt ggcttctctg
    20461 ctttgcctgg tggatacaga tatagtacat tttatagtat tggaaattat agctgtttgt
    20521 ggacaaatac agaatttgga attagcgcat ggtacagaaa aattgagcct gataaaatta
    20581 agatatttag gaatagccac aataaagaag gtggtttttc tgttagatgc attcgcgatt
    20641 aaaaaatttt tataatttaa atcatatttt ttttaaaata gaaatttaca gtattatttt
    20701 taaaacaaaa atacattgtc gtataaaatt ttgaaagcaa gtatgttttt tgaatgctta
    20761 aagttgataa cttataaaca taaaagcatt ttattagtaa cttcttactt tcgctattga
    20821 gacgtattat aatattattt tataacggat ctttaatata tttttattta atgcataata
    20881 tgattttatt atttattaca ataaaattgc tagtttatct ttgaatttaa taattattta
    20941 attttaaaat tatcaatatt tattattctc ttaattgcat agtaaaactg gaaaggtatt
    21001 tttgcaatac agaaacaaca aaaaacaaaa ttatgaaaaa aacaagtatt atcttaattt
    21061 ttatagcaat gttaatgcca ttaattggct attcacaatc aagtattaat gattcatttt
    21121 ttgatcaagt tagctataga ggtgcttttg atgatcaaaa tgactggact actggcttgg
    21181 caaattggga tccacaaaat acaaattatc cagctactac acaaactatc agtggtgaaa
    21241 taacaagtaa taccatgtgg actagtgata aagtttactt actacaagga tttgtctatg
    21301 ttgtaaatgg agttactcta actatacaac ctggcactat tattcgtggt gacaaaaata
    21361 gtaagggtac tctgataata gaacgtggtg ctaaattaat agctcaaggt actgctagtg
    21421 aaccaatagt atttacctct aatcagcccg caggttccag aaactatggt gattggggtg
    21481 gagtaatact ctgtggtagt ggcaaaatta atgtacctgg tggagaagct caaatcgaag
    21541 gtggaccacg tagctattat ggaggtacaa acgataatga taatagtggt attttaaatt
    21601 atgtaagaat agaattccct ggtatccctt tccaacctga taaagaaatc aatggtttga
    21661 ccatgggtgg tgtaggcaaa aatactacta tagactatgt gcaagtatct tatagtggag
    21721 atgatgctta tgaatggttt ggtggttgtg tgaatgctaa acatttaatt actcttggta
    21781 catgggatga tgactttgac acagattatg gattttctgg tatggtacaa tatgcagtag
    21841 cactacgcga acctaatata gcagatgtga gcggctctaa tgcttttgaa agtgataatg
    21901 acgctagtgg ttctaacaat atacctcaaa cttcagctgt tttttctaat gtatctattt
    21961 tgggtcctaa aaaagatgct tctattataa taaataataa ctataaacga gctatgcact
    22021 tgcgtagaaa tactggtatt gatatttaca attctgtctt tataggttgg tacgatggat
    22081 tgctgatcga tggctctgct tgcgaatcca atgcaaacaa tagcattcta aacatcaaat
    22141 atgtcactat ggctgctatg gctggaaact atttcactgc tgctagtgaa tcaacttttg
//
LOCUS       DAOPRN010000033        21024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_2124_length_21024_cov_94.083123, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000033 DAOPRN010000000
VERSION     DAOPRN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21024
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2124_length_21024_cov_94.083123"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PKI65_05080"
     CDS             complement(<1..244)
                     /locus_tag="PKI65_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77846.1"
                     /translation="MKKLILFILMMWISFNSTSQLYLINKTYCFITPNTYLVVNGNLN
                     NESDCKLDNAIIIVQNDITNNGTLTMVGNSTLKCGRN"
     gene            531..2696
                     /locus_tag="PKI65_05085"
     CDS             531..2696
                     /locus_tag="PKI65_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOB77847.1"
                     /translation="MRSLVSFFIIISMSISIFAQKQTKQITLENIWISNTFYPEMMGD
                     YQMYPNKDAYTIIQNNAIVLYDLKTDKIIKELLPNSVTNKAGIFIEDYSISPNANYIL
                     VFTNTRQIYRHSTESDIYLYNIKNKMLYQLPGKQNIRLAEISPDESHVAYVKENNLYI
                     YNIANNTEKAITTDGKYNFIIYGTTDWVNEEEFSFTKAFQWSHDGKYIAYYRFDESEV
                     KEFTLNFYNNDVYPEYFKYKYPKAGEDNALLDLFIYNIENDKNTKLNLGEERDIYIPR
                     IKWLKNDNQLAVIRLNRLQNHLEILLYAPDNINGKKVYDEINKAYIDITDNWFMTSDG
                     GWFYTSEKDGYNHIYYLNPTTKIDKQITSGTWDVKDIVGIDEKNTNIYYTSYEDGPIN
                     IMCYVVNYQTGNKKKIISDLGTHNPTFSSTFNYMIDAYSTINTPPEYNVYDVNKGKVV
                     REIITNEKLKNNLSEYGFVPAEFFEFTTKDNITLNGWMIKPAIEPGKHYPVLMYVYGG
                     PGVNTVKNSWGYSDFIWFQMLAQQGYIVVSVDNRGTGGRGEEFKKCTYLQLGKLESDD
                     QIEANKYLRTLPFVDSTRIGSFGWSYGGFMTALAMTKGANYFKAGISVAPVTNWKFYD
                     NIYTERFMRKPIENPDGYEKNSPIFYAKKLKGNFLLIHGDADDNVHVQNTMELVNALV
                     KNNKQFDLFIYPNKNHGIYGGYTRYHLYKKMTDFLYDNL"
     gene            complement(2791..3777)
                     /locus_tag="PKI65_05090"
     CDS             complement(2791..3777)
                     /locus_tag="PKI65_05090"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOB77848.1"
                     /translation="MKFKSVFFVLFISLLMVCCSEVKYYKWQTASEIDLIYKCENSKI
                     LIANRITKDKNLDDSQSLDYLFRGLDRQGSIATANFLLDTLKKMGFLAQYIDLPLANL
                     NKTDDTLDIYTIKNILQTNNACLLISMDYFNVYFDENIQETRQESSIDYGGATIYLAS
                     RKAVFRAILRVYDNNGLIQVFDFSHEPIYHASGNTKAEAFVNISRTQNFTINWGNAFA
                     RNIINQLFPPQMSTIRNIYVNTNKDFKIAYKYLKEKEFKKAKEIYEKYIEIDNDKILK
                     AKAIYNLAVVYELLGEPEIAISLAEQSYDIYKHDLAWSYINYLKMKRIGEDE"
     gene            3877..4758
                     /locus_tag="PKI65_05095"
     CDS             3877..4758
                     /locus_tag="PKI65_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOB77849.1"
                     /translation="MLYSMTGYGNAKLSLSNNPYEINILSLNSRNIDINVRTPQILKN
                     LEIEIRKLLTEKLIRGKIECIISYDKAAGNPIPILNESVLCNYMEQINSFTLKYKISE
                     LENPLATLLKLPGVFELEKDKLTEEELQLFFNSLTEAIDQVIDFRKQEGEKTKNDIEA
                     KISSIGKLVNEIENLKDERDNNVRTQLYELIKQLPNDIELDRNRWEQEILYYLDRLDI
                     NEELQRLKAHIEYFKNELHDEKNITKGKKLNFISQEILRETNTLGNKSNYLPIQQKVV
                     NIKDDLEKIKEQLNNVL"
     gene            4755..5324
                     /gene="gmk"
                     /locus_tag="PKI65_05100"
     CDS             4755..5324
                     /gene="gmk"
                     /locus_tag="PKI65_05100"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOB77850.1"
                     /translation="MSDKYVIVSAPSGSGKTTIIHALIAKGLPLAFSVSATSRQPRIN
                     EINGEDYYFLSLKEFKNKIANNEFVEYEEVYPGVYYGTLKNELEKIKSEGKVPIFDVD
                     VKGGMRLKNIFCDNALSLFIKPPSIEDLEKRLRNRNTEDEEILKKRLKRANYELSMEP
                     HFDYVIINDDLNRAIEEAYKIIKDFLDKN"
     gene            complement(5380..7179)
                     /locus_tag="PKI65_05105"
     CDS             complement(5380..7179)
                     /locus_tag="PKI65_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOB77851.1"
                     /translation="MAKALINIKINDIPISVEEGTSILDAAKQLNIKIPTLCDHPDLS
                     VSGNCRVCVVEVKGAKYLQAACATPVAEGMEIYTNSQVVRQSRKHVIELLLSEHNADC
                     TSCSRNGKCELQDLAAEYHIFDRILLDLLNPEDMIADISSPAIKKDISKCIHCQRCVR
                     TCEELQYVSALAVIGKGPQQHIGTFFDYPLNEVVCTNCGQCVLHCPTGALTERNYIEE
                     VWDAINDHTKHVIVQTAPAVRVAIGEELGFSYGTRVTGKMVTALKRLGFDSVLDTDFA
                     ADLTILEEGTELLTRLKRALVDKDPDVKIPMFTSCSPGWIKFMEHLYPDMLGHLSTCK
                     SPQQMFGAVAKTYYAQKKGLSAKDIIVVSIMPCTAKKYEAQRPEMRSSSYQDVDYVLT
                     TRELATMIKQAGIDFMSLPETDYDPLMGESTGAAVIFGATGGVMEAALRTAYEIVTGR
                     EVPFKNLDITPVRGFDGIREASIKIEGTKPEWSFLEGVELKVAVAHELVNANKLVRAI
                     RNGELHYHFVEVMACPGGCLGGGGQPYPVSWEVRQMRAQGIYEEDKNLLFRKSHENPE
                     IMQIYEEYLGQPNSHKAHELLHTHYYPRKKYIN"
     gene            complement(7199..8362)
                     /locus_tag="PKI65_05110"
     CDS             complement(7199..8362)
                     /locus_tag="PKI65_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOB77852.1"
                     /translation="MDTSNLKKVDLIFSKVDDPLHILDKALQKSQDDIINEIIDSGLK
                     GRGGAGFPTGLKWKFTKNEDSDVKYVICNADEGEPGTFKDRQIIEMVPEKIFIGMTIA
                     SYCVGAKEAYMYLRGEYNYLKKDLVPKMENFNNDLKNRGIDITLFLRSGSGAYICGEE
                     SALFESMEGKRGEPRNKPPYPTKSGYNYKPTVINNVETLVYVTMIEYIGVEEFRKLGT
                     KDSRGSKVFSISGDTPIPGIYELELGMKVSDFVREFGDGDTKAVQIGGASGFCVPRKN
                     FESTIIGFEGIPTGGSVMLFNSSRSMYNVLHNYLDFFVEESCAQCTPCRAGLQQLLKG
                     IEAIKRREKPVSYLDELIKLSKTMKVASKCGLGQSIANPFVSIVENFKEEIIF"
     gene            complement(8370..8843)
                     /gene="nuoE"
                     /locus_tag="PKI65_05115"
     CDS             complement(8370..8843)
                     /gene="nuoE"
                     /locus_tag="PKI65_05115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOB77853.1"
                     /translation="MEESKELIKKSVAKHGKSRNALMPILQDIVADKQYLEPLDIALV
                     AEELDLSAADVYGTATFYTFLDTVPRGKNVIRVCRTISCYMSGKDEIISTLEKILNIK
                     VGETTIDRQFTLLTANCMGWCHKGPVMLINDEVYHSLTPEKTIEIIQQYREKSKN"
     gene            complement(8958..10451)
                     /locus_tag="PKI65_05120"
     CDS             complement(8958..10451)
                     /locus_tag="PKI65_05120"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77854.1"
                     /translation="MKYTAFLIYTFLFVNILFSQDSIKKLINYYNKDQISSIGYMKDN
                     VPHGYWINYHINGNIRSEGKFVNGLLDSTWKYYNEDGKISQVINYNMGLLHGDWYRYY
                     DKYFIKCKYVNNKLEDTCFTFTNSNKLMRTIPYNNGVPDGIGYEYDTTDNRIITIYTY
                     KNGFLLSRESINRYDDNGKKTGKWIEVDSTKKLKHEVHYNNGLKYGISKTYDLKTDKL
                     ISIKRYQADSVIVTSDVKIHEIKRDYYPDGKIKSIGSYYNGVPDGVRRDYDNDGTIIA
                     SYIFKDGILEGTGIIDEKGKYQGKWYFFYENGKVLETGYYRDGKKSGEWIVYDTLGNI
                     IEVMNYFNGKLDGEYIFYFYNGNVKRKIYYQDGVAEGEYVEYDIEGNVISKGKMIGDE
                     RMGKWIYRVNDLLYEVEFSNDILNGKYEIRNIKNKQKLYNGKYFDGKPINKHYRYYDN
                     KELQLIESYDIAGERNGWEKYFNTQGYLIYKVFYKLGNVDKIGNKKL"
     gene            10577..12154
                     /locus_tag="PKI65_05125"
     CDS             10577..12154
                     /locus_tag="PKI65_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOB77855.1"
                     /translation="MKIHFILISLLSFLLICCATVEMPSGGPIDKTPPKLITVTPRDS
                     SIHFSYDKIIFIFDEFIKPVTKNKVNINPYLSLDKYEIATQGKKVIITFDDTLSSNTT
                     YTITFNEAIIDITEGNILKRLTYSFSTGDFIDTCTLSGKAILSESDLPMSKGVVILND
                     RADFNWDSPPLYLDYCLDGIFLFTNLPTDSFYLYTLEDLNNNLMYEINKEHVGFYENI
                     INLSRDNNYSDIEVLNFIEFPKNKRIISNPRYNLNNTLIIFNQPLNSPLVAIIPDTLN
                     ILSYWNENKDTLSIFYPITSWDTTKITIKDFGLDTTINILTSLKGTQKTSKDTVLKLS
                     LISYDSQLLPSDSCIIKGSLPFKWINTDSIILIKNEKDTMPLSLNENYALYHTIIDKL
                     PNGEYELIIKNNAAYSIAGTTNKEEKIRFSKLSTREVSMLEIKFSNALKWDGIIKLNG
                     KYFKRSYLVPIGTSEFIINELLPDNYELMIVKDDNNNGKYDTGNFEKRILPERVYRFP
                     KKVEIIKNWDQSIIWDL"
     gene            complement(12301..13818)
                     /locus_tag="PKI65_05130"
     CDS             complement(12301..13818)
                     /locus_tag="PKI65_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOB77856.1"
                     /translation="MNKINKDRWVKYILIFFAFVIVIGSLGYMNSIMKQIAISEQKNI
                     ELWAKTIQKRANLLKETQDFLTMLEIEERQKMNIWAEATKRIIKAASGDDLSFYADII
                     SSNNSIPILMVNENNRILASKNVNPPYDTLKYLSKENASKFFIYPPIEIDLGKTKHYL
                     YYADSRLIEKFHYIINDIVEKFLDDIVENNINIPVIVTDTTRTKIYACGNIDTCALTD
                     NAYMKKLINSMESENKPITIQLPNGEKQLIFYKTSQVISKMRWFILLQLAIILVFVLI
                     VIFFSRALYLSERDSIWVGMAKETAHQMGTPLSSLFAWVEILEAEYGPSETISEMRKD
                     LNRLQRVSDRFNIIGSQPNFTEVSIIELLMEIHQYFLHRIPQKKIDLKLYIEPQEDVV
                     LPVNAELLSWAIENIVKNAIDSIDIEKEKQVVEIKLSVNEKYAFIDISDTGKGISSKI
                     QKYIFKPGFSTKEHGWGLGLALAKRIVEEYHKGKILVSSSTNNGTKITIQLNKTL"
     gene            complement(13891..14319)
                     /locus_tag="PKI65_05135"
     CDS             complement(13891..14319)
                     /locus_tag="PKI65_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77857.1"
                     /translation="MEINSEIIKPPYPVSIIMNYLSDINSLSRLIESHREEIDDNDLS
                     SNINNINIEGNQITLDTDQGSIYVSLIDTASNKVTYKLNINNMKNLNEIIILFNSDPI
                     ANTLWLTINIDIPYVMGLMLKPKIKEFMNEINTRLSQSIE"
     gene            complement(14375..15694)
                     /locus_tag="PKI65_05140"
     CDS             complement(14375..15694)
                     /locus_tag="PKI65_05140"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOB77858.1"
                     /translation="MKSILVIGAGFSAKVLIDYLSKQAKEKNWNITVAEQNLELLEYL
                     DIPKIKLDVTDDQARYECIKNFDIIVSMVPAKYHILIAKDCLALSKHLFTASYISPEM
                     KALDNEVKSKKLLFLNECGLDPGLDHMSAMKIINDIKDKDGEILEFESSTGGLVAPES
                     DDNPWNYKFTWNPRNVVLAGMEPAKFLENGQYKYIPYHRIFQWTKPINISGLGDFEVY
                     PNRDSLKYRFVYGLDNVKSMLRGTIRRPGYCKAWDGFVQLGITDDSYEIENIDKMTWR
                     ELIASYLPNIPEKTLEDIFCYYVGIEKNSPEFHKFEWLDIFTDKLVGLQKATPAQALQ
                     HLLEEKWKFGDNDKDLVVMQHKVIYHLNNKKHVKKSSFQYIGKDIKNTAMATTVGLPL
                     AIAVELFIEGKIDKSGVLLPTDPDIYDPILEKLKDFDINFLEVDEIL"
     gene            complement(15697..17010)
                     /locus_tag="PKI65_05145"
     CDS             complement(15697..17010)
                     /locus_tag="PKI65_05145"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysine ketoglutarate reductase
                     /saccharopine dehydrogenase family protein"
                     /protein_id="HOB77859.1"
                     /translation="MNLGIRAETKYPSEKRVAVIPEHVNKLVKQGFKVLVQSSNKRIF
                     TDEEYEKAGAKIVNSLEQADIIIGIKEIPEEEIYADKVYLFFSHTIKGQKHNIPMLKQ
                     LIKQRATLIDYEKIVDDFGKRLIFFGNYAGNAGLVDTLWMVGKRLVYKGIANPFDKLK
                     RAFEYSNLDNIIASMSEIGFDILKNGLPESLIPFIIGITGYGNVSKGVQEILHFLPVK
                     EILPEDLKRFSDIPPSPHHIYKVVFKEKDMVKRRDGGKFDLNDYYLHPENYESIFYQY
                     LPYLNVLIHASYWDQKYPRVVEEKYIIDNWGKPDFRLLGIGDISCDINGGVEITKTST
                     EVENPVLIYNPATRDYIIDLYSEGLPVLAVDILPTELPMDASTYFSSILLPYIEALLK
                     ANWKKNFNKLEIPLPLKRGTILYKGKFTPDYEYMKQFINNNQKKQ"
     gene            complement(17076..18404)
                     /gene="rseP"
                     /locus_tag="PKI65_05150"
     CDS             complement(17076..18404)
                     /gene="rseP"
                     /locus_tag="PKI65_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOB77860.1"
                     /translation="MTVIIKILQLLFALSILILVHEFGHFLFAKIFKVKVEKFYIFFD
                     YKFSLLKFNYKGTEYGIGWIPLGGYTKIAGMVDESMDTDHLKNEPQPWEFRSKKAWQR
                     FLILFGGVLMNVLLAIVINIFILWNIGEQYLPNSSLKYGLAVDSTFQAIGLRNGDLVV
                     AIDDHPVNSFTEIIPTLLLEKGNTIIVQRGDSLVYIPVPSDFLNKLIKQDEPIQFFFP
                     RFPFIIDSVVENSAAALAGLQKGDKIIGIQDTITPYFTDIKALLQNYKDQEVDLWVVR
                     NNDTITLNVAVPSTGIIGVMPVADLSQFFEIYSYKYTFWQSIPRGFNRTWEIINSYVK
                     QLGLIFSKEVKGYEGLGGFITMGKIFPGQWNWLAFWNLTAFLSIMLAVLNIIPIPGLD
                     GGHILFVLVEMITGRKPSEKFLIGAQFIGLMFLLALVIYANLNDIIRLFR"
     gene            complement(18411..19574)
                     /locus_tag="PKI65_05155"
     CDS             complement(18411..19574)
                     /locus_tag="PKI65_05155"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOB77861.1"
                     /translation="MKEKIKLAILGSTGSIGRQTIEVVEMFPEIFEIEVLTANENVEL
                     LAKQSLEHHPNAVVISNQELYLELKDMLKDTDIKVFSGADSINHIAKSDNIDIVIIAL
                     VGFAGLQPTLEAAKAGKRIALATKEALVVAGELVINEVKQGKAFLIPIDSEHSAIFQC
                     LQGEALNTVSQLILTASGGPFKNLKFEDFDNITIDEALRHPRWQMGNKITIDSSTLMN
                     KGFEVIEAHWLFGIAPSNIKVLIHPESIIHSMVQFIDGSIKAQLSIPDMRIPILYALT
                     YPYRLPTNFDTPDFVELHSLSFDNPDIKHFPHLQLAYDILNIGGIMPCVLNAANEIAV
                     YAFLNKEIKFTQMYTVIEKTLEKFTPTSVENVEHLISVDQRARKISCEIINQI"
     gene            complement(19571..20332)
                     /locus_tag="PKI65_05160"
     CDS             complement(19571..20332)
                     /locus_tag="PKI65_05160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOB77862.1"
                     /translation="MKKNVKITPKRLFRNFRHPYILILRRLDNLEVIFTIYLTRLKFF
                     LFLALFVLLLIIVTTIIIAYTPLRQYIPGYGDIKEKEMAYSLAKKTDSLVEILNQREV
                     YYYNLHNLLMTMDTSYNKTLRAFFYKTENVNLNNIKLSYPLSTPKIIKKYGEAPMPMN
                     VRLESKSSEIVKAMADGTVIDTGMISHINGKQVIIQHNNNMISIYKNLDKVNVGIGVQ
                     VKNNSNIGNAKPEKSFIDISVFLDGNAVNPEIFWK"
     gene            complement(20541..20723)
                     /locus_tag="PKI65_05165"
     CDS             complement(20541..20723)
                     /locus_tag="PKI65_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77863.1"
                     /translation="MRRFRGVATKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
BASE COUNT         7390 a         3394 c         2747 g         7493 t
ORIGIN      
        1 aatttcttcc acattttagt gtagagttac ctaccattgt caatgtgcca ttattagtaa
       61 tgtcattttg tacaataata atagcattat ctagtttaca atcgctttca ttatttagat
      121 ttccattgac aactagataa gtattagggg tgataaaaca atatgtttta tttataagat
      181 aaagttgaga ggtggaatta aaactgatcc acatcattaa tataaataaa attaattttt
      241 tcataacaaa aatattttat atattacaaa tttacaaaat atatttatat ttttataaat
      301 aattttttat ttttaaacta aatatttatg taaaattaca aaaatatttt aattttattt
      361 ataatatttt taaaatataa gtctactacg aaaattcaaa aaaactatat atcactacat
      421 taccattatt cttttaaata tatctgatat ttatttgata aaaaacataa actttttata
      481 attttttatg tgagccgaaa aaaattatta ttttagcata aaaattcatt atgcgttcat
      541 tagttagttt ttttattatt atttcaatga gtatatctat ttttgctcag aaacaaacca
      601 aacaaataac cttagagaat atatggataa gtaatacatt ctacccagaa atgatgggag
      661 attatcagat gtatcctaat aaagatgcat atactataat ccaaaataat gctatcgtat
      721 tatatgattt aaaaactgat aaaatcatta aagaattgct accaaattct gttactaata
      781 aagctggtat ctttatagaa gactattcga tctctccaaa tgctaattat attcttgttt
      841 tcactaatac caggcaaata tacagacata gtactgagag tgatatttat ctatacaata
      901 tcaaaaataa aatgctatat caattaccag gcaagcaaaa tataagatta gctgagatat
      961 ctccagacga atctcatgtt gcctatgtga aagaaaataa tttatatatt tataacatag
     1021 ctaataatac cgaaaaagca ataactactg atggtaaata caattttatc atttatggta
     1081 ctacagattg ggtaaatgaa gaagaatttt cttttacaaa agcttttcaa tggtctcacg
     1141 atggtaaata tatagcatat tatagatttg atgaatcaga ggtaaaagaa ttcacattaa
     1201 atttttataa taatgatgtc tacccagaat actttaaata caaatatcct aaagctggtg
     1261 aagataacgc actattagat ttattcatct ataatataga aaatgataaa aacacaaaat
     1321 taaatttagg tgaagagcga gatatataca tacctagaat taaatggtta aaaaatgata
     1381 atcaattagc agtgatcaga ctcaaccgtt tacaaaatca tttagaaatt ttactatatg
     1441 cacctgataa tattaatggg aaaaaagttt atgatgagat aaacaaagct tatatcgata
     1501 tcacagacaa ttggtttatg acctctgatg gtggctggtt ttatacttcc gaaaaagatg
     1561 gatacaatca tatttattac ttaaatccta caaccaaaat tgataaacag ataactagtg
     1621 gtacatggga tgtaaaagat attgtaggta tagatgagaa aaataccaat atttattata
     1681 cctcttacga agatggtccg ataaatataa tgtgctatgt agtcaattat cagactggca
     1741 acaaaaagaa aataatatca gatctaggca ctcacaaccc tactttttca tcaactttta
     1801 attatatgat cgatgcttac tctactataa atacaccccc agaatataat gtttatgatg
     1861 taaataaagg taaagtagta agagagataa taactaatga aaagctaaaa aataatttgt
     1921 cagaatatgg ttttgtacca gcagagtttt tcgaattcac tactaaagat aatataacct
     1981 tgaatggttg gatgataaaa ccagctatag aaccaggcaa gcattaccct gtattaatgt
     2041 acgtttatgg tgggccaggt gtaaatacag taaaaaatag ttgggggtac tctgatttta
     2101 tttggtttca aatgttggca cagcaaggtt acatagtagt aagtgtagat aatcgtggca
     2161 cgggcggtag aggtgaagaa tttaaaaaat gcacatatct acaacttggc aaattagaaa
     2221 gtgatgacca gatagaagca aataaatatt tgagaactct accttttgta gactctacac
     2281 gcattggttc gttcggatgg agctatggag gatttatgac tgcattagct atgactaaag
     2341 gtgctaatta tttcaaagct ggtataagcg tagctccagt aaccaattgg aaattttatg
     2401 ataatattta tactgaaaga tttatgcgaa aaccaattga aaatccagat ggttacgaaa
     2461 aaaattcacc aattttttat gctaaaaaat tgaaaggtaa ttttctacta attcatggcg
     2521 atgctgatga caacgtgcat gtacaaaaca caatggagct cgtgaatgct ttagtgaaaa
     2581 acaataaaca atttgattta tttatatatc caaataaaaa tcatggtatc tacggtgggt
     2641 atactcgcta tcatttatat aagaagatga ctgacttttt atatgataat ttgtaaagat
     2701 ttttttttag atcttcaaac aatgccccac aactgacaac ttacgttttg ctaagtttct
     2761 atacttttca tcatcgcctt gtctatcatc ctactcatct tcgcctattc tcttcatttt
     2821 cagataatta atatacgacc aagctaagtc gtgtttataa atatcataac tttgctcagc
     2881 taaagaaatt gctatttctg gttcacctaa tagctcgtat actactgcca aattataaat
     2941 agctttagcc tttagtattt tatcattatc tatctctata tatttttcat agatttcttt
     3001 tgctttttta aattcttttt cctttaaata tttataagcg attttgaaat ctttattagt
     3061 gtttacataa atatttctaa tagtactcat ttgtggaggg aaaagctgat taataatatt
     3121 acgagcaaaa gcattacccc agtttattgt aaaattttgt gttctagaga tattaacaaa
     3181 agcttctgct ttagtattgc cacttgcatg atatataggt tcatgactaa aatcaaaaac
     3241 ttgaatcaat ccattattat cataaactct tagaatagct ctaaatacgg ctttgcgaga
     3301 agccagatat attgtagcac caccatagtc tatagagctt tcttgtctag tttcttgtat
     3361 attttcatcg aaatacacat tgaaataatc catacttatt agcaagcaag cattattagt
     3421 ttgtaatata tttttaatag tatagatatc caaagtatca tcagttttat ttaaattggc
     3481 aagtggcaaa tctatatatt gagccagaaa gcccattttt tttaatgtat ctaaaagaaa
     3541 attggcagta gcaatagatc cttgacgatc taaccctcta aaaagataat ctaatgattg
     3601 tgagtcgtct agatttttat ctttagttat cctattagca ataagtattt tagaattttc
     3661 acacttatat attaaatcta tttcagatgc agtttgccac ttataatatt tgacttctga
     3721 gcagcaaacc attaataatg aaataaataa aacaaaaaat acagacttaa atttcattat
     3781 cattaaaaat ttaaaaaaat aaaaacaaaa tatcaaacaa agataagtga aattttttta
     3841 aattataaaa tatacttagt aaatttgcta aaaaatatgt tatattcaat gacaggttat
     3901 ggcaatgcta aacttagctt atcaaacaat ccttatgaaa tcaatatatt aagtttaaac
     3961 tctagaaata ttgatataaa tgtccgcacg cctcaaatat taaaaaattt agaaatagaa
     4021 atacgtaaat tattaactga aaaattgata agaggcaaaa tagaatgtat cattagctat
     4081 gataaagcag caggcaatcc aatacctata cttaatgaat cagttttatg taattatatg
     4141 gagcaaataa atagtttcac tttaaaatat aaaatatctg agctcgaaaa tccactcgct
     4201 acacttttaa agttgccagg tgtttttgaa ttagaaaaag ataaattgac agaggaagaa
     4261 ttacaactat tttttaatag tctaactgaa gccattgatc aagtgataga tttcagaaaa
     4321 caagaaggtg aaaaaaccaa aaatgatata gaagcaaaaa tatcaagtat aggtaaatta
     4381 gtaaatgaaa tagaaaattt aaaagatgaa agagataata atgtaagaac tcaattatat
     4441 gaactaataa agcaattacc aaatgatata gaattagatc gtaatagatg ggaacaagaa
     4501 attttatatt atttggatag gttagatata aatgaggaac tgcaaagact gaaagctcat
     4561 atcgaatatt tcaaaaatga attacatgat gaaaaaaata tcaccaaagg caaaaaatta
     4621 aattttattt ctcaggaaat tttacgagag actaatactc taggaaacaa aagtaattat
     4681 ttgcccatac agcaaaaagt agtgaatata aaagatgatt tagaaaaaat aaaagaacag
     4741 ctaaataatg ttttatgagt gataaatatg tgatagtatc cgcaccaagc ggttctggca
     4801 aaactacaat catacatgca ctaatagcta aaggcctacc tttagctttt tcggtatcag
     4861 ctactagtcg ccaaccaaga ataaatgaaa taaatggaga agattattat tttttatctt
     4921 taaaagaatt taaaaacaaa attgcaaata atgagtttgt agaatacgaa gaagtatatc
     4981 caggtgttta ctatggcaca ttgaaaaatg aattggaaaa aataaaatca gaagggaagg
     5041 tgccaatatt cgacgtagat gtaaagggcg gtatgagact aaaaaatatc ttttgtgata
     5101 atgctttatc actatttatc aagcctccta gcatcgaaga tttagaaaaa agattaagaa
     5161 atcgcaatac tgaagatgaa gagatattaa aaaaaagatt aaaacgggct aattatgagc
     5221 tatccatgga accacatttt gattacgtta ttataaacga tgacttaaat agagcaatag
     5281 aagaggcata caagattata aaagattttt tagataaaaa ttaaagaatc tatttctaaa
     5341 acaagctaat tgaaaacttg aatagtttta tcattttttt taatttatat atttttttct
     5401 aggataataa tgagtgtgaa gcaattcatg agctttgtga ctattaggtt gtcctaagta
     5461 ttcctcgtat atctgcatta tttcaggatt ttcatgcgat ttacggaaaa gtaagttttt
     5521 atcttcctca taaatacctt gagcacgcat ttgtcttacc tcccaagaga cagggtaagg
     5581 ttggccacca ccaccgaggc aaccaccagg acaagccatc acttctacga aatgataatg
     5641 taattctcca ttcctaatag ctctaaccaa tttattagca tttactaact catgagctac
     5701 ggcaactttt agttctacac cttctagaaa tgaccattcg ggcttagtac cttctatttt
     5761 gatactagct tctctaatac catcaaatcc acgtactggg gtaatgtcta aatttttaaa
     5821 aggtacttct cgtcctgtaa caatttcata agctgtacga agtgcagctt ccattacccc
     5881 acctgtagca ccaaaaatta cagcagcgcc agtagactca cccatcaacg gatcgtagtc
     5941 agtttcaggt aagctcataa aatctattcc agcttgttta atcatagtag ctagctcacg
     6001 agtagtaagt acataatcta catcttgata actactgcta cgcatttcag ggcgttgggc
     6061 ttcatatttt ttagcagtac aaggcataat agacactaca ataatgtctt tagcacttaa
     6121 gccttttttt tgtgcataat aagtcttagc tacagcccca aacatttgct gtggtgattt
     6181 gcaagtagat agatgaccaa gcatatcagg gtataaatgc tccataaact taatccaacc
     6241 aggagagcag ctagtaaaca taggaatttt aacatctgga tccttatcta ctaaggctct
     6301 ttttagtctc gttaaaagtt cagtgccttc ttctaaaatg gttaagtccg ctgcaaaatc
     6361 tgtatctaaa acagaatcaa aacctaaacg tttaagagca gttaccattt taccagtaac
     6421 tctagtacca taagagaacc ctaattcttc gcctatagcc acacgtactg ctggggcagt
     6481 ttgtactatt acatgtttag tatgatcatt aattgcatcc catacttctt caatataatt
     6541 acgttcagtc aaagctccag taggacaatg taacacacat tgcccacaat tagtacatac
     6601 tacctcattt aatgggtaat caaaaaaagt ccctatatgt tgctgaggtc ctttacctat
     6661 cactgctaat gcactaacat attgtagttc ctcacaagtg cgtacgcaac gctgacaatg
     6721 aatacattta gagatatctt ttttaatagc tggagaacta atatctgcaa tcatatcttc
     6781 gggatttaaa agatctaata atattctatc aaagatatgg tactcagcgg ctaaatcttg
     6841 taattcacat tttccatttc tggagcaact agtacaatct gcattatgtt cagaaagtaa
     6901 taattctata acatgcttac gagattgcct gactacttgg ctatttgtat atatttccat
     6961 accctctgct acaggagtag cacaggctgc ttgcaagtat ttagcacctt tcacttctac
     7021 tacgcaaact ctacaattgc cagagacact caaatctgga tgatcgcata gcgtaggaat
     7081 tttgatattt aactgtttag cagcatctaa aatagatgta ccttcttcta cagatattgg
     7141 aatgtcattt attttaatat ttattaatgc ttttgccata attattattt tttttaattt
     7201 aaaatattat ttcttcttta aaattctcta caatagaaac aaatggattt gctattgatt
     7261 ggcctaatcc acatttagaa gctactttca tagttttaga gagttttatt aattcatcta
     7321 gatagcttac tggtttttcg cgtcttttaa tagcttctat accttttaat aattgttgta
     7381 agccagctct acaaggtgta cattgagcac agctttcttc tacgaagaaa tccagataat
     7441 tatgtaaaac attatacata gatctagaac tattaaataa cataacagag cccccggtag
     7501 gtatgccttc gaaacctatt atagtacttt cgaaattttt tcttggtaca caaaaaccag
     7561 aagcaccgcc tatttgtaca gctttagtat cgccatcgcc aaattctctt acaaaatcag
     7621 atactttcat gccaagctct aattcataaa tacctggaat aggggtatcg ccagatatag
     7681 aaaatacttt agaaccacga gaatctttag tgcctaattt tcgaaattct tctaccccta
     7741 tatattctat catagtaaca taaactaaag tttctacatt atttataact gtaggtttat
     7801 aattataacc tgatttagta ggataaggtg gtttatttct aggttcacct cgtttacctt
     7861 ccatactttc gaataaggca ctttcttcgc cacaaatata cgcaccacta cccgaacgca
     7921 agaataatgt gatatcaata cctctatttt ttaagtcatt attaaaattt tccattttag
     7981 gtactaaatc tttttttaga taattatatt cgcctcttag gtacatataa gcttctttag
     8041 cacccacgca atatgaagct atagtcatac cgataaagat tttttcggga accatttcaa
     8101 tgatttgacg atctttgaaa gtaccaggct caccttcatc agcattacaa atcacatatt
     8161 tcacatcgct atcttcgttt ttagtaaatt tccatttcaa tcccgttgga aaacctgcac
     8221 ccccacgtcc ttttagtcca ctatcaatta tttcatttat tatatcatct tgtgatttct
     8281 gcaaagcttt atccaatata tgtaacggat cgtctacttt gctaaaaata agatctactt
     8341 tttttaaatt acttgtatcc atactaattt caattttttg atttttctct atattgttgt
     8401 atgatttcaa tagttttttc aggtgttaga ctgtgatata cctcatcatt tattagcatc
     8461 actggtcctt tatgacacca acccatacaa ttagcagtta gaagtgtaaa ttgcctatca
     8521 atagtagtct cacctacttt aatattcaaa attttttcta aagtacttat tatctcatct
     8581 ttaccagaca tatagcaact aatagttctg caaactctaa ttacattttt acctctaggg
     8641 actgtatcaa gaaaagtata aaaagtagct gtaccataaa catctgcagc agataaatct
     8701 agctcttcgg ccactaaagc tatatctaat ggttctaaat attgcttatc tgcaactata
     8761 tcttgcaaga taggcatcaa agcattacga cttttgccat gctttgctac tgattttttg
     8821 attaattctt ttgactcttc cataatattc ttttgtttta ttgttaatta attaacaatg
     8881 caaagttata tataattttt tatatataaa caataaaata aaacaaatct atatgtaaat
     8941 tttgaaatta atgtagatta caatttttta ttacctattt tatctacatt acctaattta
     9001 tagaaaacct tgtaaattaa atatccttgt gtattaaaat atttttccca cccatttcgc
     9061 tcacctgcaa tgtcgtagct ttctattaat tgtagttctt tattatcata atatctataa
     9121 tgcttattta ttggcttacc atcaaaatat ttaccattat aaagtttttg tttattttta
     9181 atatttctta tttcatattt tccatttaaa atatcattag aaaattctac ttcgtataat
     9241 aaatcattaa ctctataaat ccacttaccc attctctcat caccaatcat ttttcctttt
     9301 gatatcacat ttccctcaat atcatattct acatattcac cttctgctac accgtcttgg
     9361 taatatattt ttcgttttac attaccatta taaaagtaaa aaatatattc accatctaat
     9421 tttccattaa aataattcat tacttcaata atatttccta aagtatcata aactatccat
     9481 tctccagatt tttttccatc tcgataataa ccagtttcta aaactttacc attttcataa
     9541 aaaaaatacc attttccttg atacttacct ttttcatcta ttatacctgt accttctaga
     9601 atgccatcct taaaaatata tgaagctatt attgtaccat cattatcata atctcgcctc
     9661 actccatcag gtactccatt ataatagctg ccgatagatt ttattttgcc atcaggataa
     9721 taatcccttt tgatttcatg tattttcaca tctgaggtta ctatcacaga atcagcttga
     9781 tatcttttaa tacttattaa tttatcagtc tttagatcgt aggttttcga aataccatat
     9841 ttcaatccat tattataatg tacttcgtgt tttaattttt tagtactatc tacctctatc
     9901 catttgccag tttttttacc attatcatca tacctattaa tagattcgcg actcaataaa
     9961 aatccatttt tataagtata aatagtaatt atacgattgt cggtagtatc atattcataa
    10021 ccaataccat caggtacacc attattatat ggtatagttc gcatcaattt attagaatta
    10081 gtaaatgtaa aacaagtatc ttctaattta ttattaacat atttacattt gataaaatat
    10141 ttatcataat atctatacca atctccatgc aataatccca tattataatt aataacttga
    10201 gaaattttac catcctcatt ataatatttc caagtgctat ctagtaaacc atttacaaat
    10261 ttgccttctg atctaatatt accattaata tgataattaa tccaatatcc atgcggtaca
    10321 ttatctttca tgtatcctat actacttatt tgatctttat tataataatt aattagtttt
    10381 ttaatagaat cttgtgaaaa taaaatatta acaaataaaa aggtataaat taaaaatgct
    10441 gtatatttca tcgaaaatca attattattt ccaaaattat gaaatttttt attaaatgaa
    10501 acctaatact tttttattaa ttttgttaaa aaaaaactaa ttcggtccat atctgatata
    10561 gacaaataaa tatattatga aaatacattt tatattaatt tcattactat cttttttgct
    10621 aatttgctgt gccacagtgg agatgccaag tggcggtcct atagacaaaa cacctccaaa
    10681 attaattaca gtaacccccc gagattcttc tattcatttt tcttatgata aaataatatt
    10741 tatattcgat gaattcataa aacctgtaac caaaaacaag gtcaatataa atccatacct
    10801 atctttggat aaatatgaaa tagctacaca aggcaaaaaa gttataatta catttgatga
    10861 tacattatct agcaatacta cctacactat aacttttaac gaagcaataa tagacatcac
    10921 cgaaggcaat attttgaagc gtttgactta tagtttttct actggtgatt tcatagatac
    10981 atgcacatta tccggcaaag ctatattatc cgaatctgat ctacccatga gtaaaggtgt
    11041 agtaatatta aatgatagag ctgattttaa ctgggattca ccacctcttt atttagatta
    11101 ttgcttagat ggtatttttt tatttactaa tttacctact gatagttttt atttatatac
    11161 attagaagat ttgaataata atttgatgta tgagattaat aaggagcatg tcggttttta
    11221 tgaaaatata attaatttat caagagataa taattattca gatatagaag ttttgaattt
    11281 catagaattt cctaaaaata aacgaatcat aagcaatccc aggtataatt tgaataatac
    11341 attgattatt tttaatcagc cactcaatag tcctttggtt gccatcatcc ctgatacatt
    11401 aaatattctg tcatattgga atgaaaataa agatacgctt tcgatttttt acccgattac
    11461 cagctgggac acaactaaaa taactattaa agatttcgga ttagatacta caataaatat
    11521 tttgactagt ctaaaaggaa cacaaaaaac atctaaggat acagttttga aactaagcct
    11581 cataagttat gattcacaat tactgccatc cgattcctgc attatcaaag gttctttacc
    11641 ctttaaatgg attaatacag attctataat tttaataaaa aatgaaaaag atacaatgcc
    11701 attatcatta aatgaaaatt atgctttgta tcatactatc atagataaac taccaaatgg
    11761 tgaatatgaa ttgataataa aaaataatgc agcatattcc atagctggta caactaataa
    11821 agaagaaaaa attagatttt caaagttatc tacacgagaa gtttctatgt tagaaataaa
    11881 attttcaaat gcactcaagt gggatggtat tataaaacta aatggcaaat attttaaaag
    11941 aagctatcta gtaccaatag ggactagtga attcattata aatgaattat taccagacaa
    12001 ttatgaactt atgatagtaa aagatgataa taataatggc aaatatgata caggtaactt
    12061 cgaaaagcgt atcttgcctg aacgagtata tcgatttccc aaaaaagtag aaattatcaa
    12121 aaattgggat caatcgatta tatgggattt atagagcaat aaggatatat acctatatat
    12181 tattgctact acatttctca tctttttttt ctctctcagc tattcctact ttactttcta
    12241 taatattttt aataaatata attagagata ttctaattaa tatatttata aagaatcatc
    12301 ttacaaagtt ttatttaatt gtattgtgat tttagtacca ttatttgtag atgatgatac
    12361 taatattttt cctttatgat attcttcaac tattctttta gccagagcca atccgagtcc
    12421 ccacccatgt tctttagtag aaaaacctgg tttgaatata tacttctgta ttttcgaaga
    12481 tatacctttg ccagtatctg agatatctat aaatgcatat ttttcattta ccgaaagctt
    12541 tatctctact acttgttttt ctttttctat atctatagaa tcaatggcat tttttacaat
    12601 attttctatt gcccatgaca gtaattcagc atttactggc aaaacaacat cttcttgcgg
    12661 ctctatatat agttttaaat ctatcttttt ctgtggtatt ctatgcaaaa aatattgatg
    12721 tatctccatt aaaagctcta ttattgatac ctcagtaaaa ttaggttgag atccaataat
    12781 attaaatcta tcacttacgc gttgaagtcg attaagatct ttacgcatct cacttatagt
    12841 ttctgatggt ccatactcag cttctaaaat ttctacccaa gcaaataatg acgacaaagg
    12901 ggtccccatt tgatgagcag tttccttagc catacctacc cagatagaat ctcgctctga
    12961 aagatataaa gctcttgaaa aaaatataac aattaataca aaaaccaaaa taatagctaa
    13021 ttgtaacaag ataaaccatc tcattttact aattacttgt gatgttttat aaaagattaa
    13081 ttgtttctct ccatttggca gctgaatagt tataggtttg ttttcagact ccatagagtt
    13141 gatcagcttt ttcatatatg cattatcagt gagagcacaa gtatctatat tgccacaagc
    13201 ataaattttt gttcgggtag tatcagtaac tattacaggt atgttaatat tattttctac
    13261 aatatcatct aaaaattttt cgactatatc atttatgata taatgaaatt tttctatcaa
    13321 tcgagaatct gcataatata agtaatgttt ggtttttcct aaatctattt ctataggagg
    13381 gtaaataaaa aatttacttg cattttcttt gctaagatac tttaaagtat catagggggg
    13441 atttacattt ttactggcta atattctatt attctcgttt accatcaaaa tgggtataga
    13501 gttattagag cttatgatat cagcataaaa agataaatca tcaccacttg ctgcttttat
    13561 tatacgttta gtagcctctg cccatatatt cattttttga cgctcttcga tttctaacat
    13621 tgtcaaaaaa tcttgtgttt cttttaatag atttgctctt ttttgaattg ttttagccca
    13681 taattctata ttcttttgct cagatatagc tatttgcttc attatagagt tcatatatcc
    13741 cagactgcct attactataa caaaagcaaa aaaaatcaat atatatttta cccacctatc
    13801 tttattgatt ttattcatca atttttttat ttcagtttta acaatataaa tgatttaata
    13861 atattatcaa attacttata aaaagataat ttattcaata ctttgagata atctagtatt
    13921 tatttcgttc ataaattcct ttatttttgg ctttagcatt aatcccatta catagggaat
    13981 gtctatattt attgttagcc atagagtatt agctatcgga tcactattaa ataatatgat
    14041 gatttcgttt aaatttttca tattattaat atttagttta taagtaacct tattagatgc
    14101 agtatctatt agacttacat aaatcgagcc ttgatcagta tctaacgtta tttgattacc
    14161 ttcgatattg atattattga tattactcga caaatcatta tcgtctatct cttccctgtg
    14221 agactctatt agtctcgaga gactattaat atcagaaaga taattcatga taatagaaac
    14281 aggatagggc ggtttaatta tttcactatt tatttccatt ttttatattt tttttaaaaa
    14341 aatttagcta taatgtatat gcattgcagt gtatctataa gatttcatcc acctccaaaa
    14401 aattaatatc aaaatctttt aatttttcta atataggatc ataaatatca ggatctgtag
    14461 gtaacaagac accagattta tcgattttac cttctataaa tagctctact gcaatagcga
    14521 gaggtaggcc aaccgtagta gccatagctg tatttttaat atctttacct atgtattgaa
    14581 atgaggattt ttttacgtgc tttttattat ttagatgata tataaccttg tgctgcatca
    14641 ctactaaatc tttatcatta tcaccaaatt tccatttttc ttctaataaa tgttgtaatg
    14701 cttgagcagg cgtagccttt tgtagtccta caagtttatc agtaaagata tctaaccatt
    14761 caaatttatg aaattcagga ctattttttt ctatacctac ataatagcaa aagatatctt
    14821 ctaatgtttt ttcaggaata ttaggaaggt aagaagctat tagttctctc caagtcattt
    14881 tatcaatatt ttctatttca taactatcgt cagtgatacc gagctgcaca aatccatccc
    14941 atgctttgca ataccctggc cgcctaatag tgcctctcag catagatttt acattatcta
    15001 atccatatac aaatctatat tttaatgaat ctctattagg atatacttcg aaatcaccta
    15061 aacctgaaat attaattggt ttggtccatt gaaaaattcg atgatatgga atgtatttgt
    15121 attgaccatt ttctaaaaat tttgctggct ccatacctgc caaaaccaca ttacgtggat
    15181 tccaagtgaa tttataattc caaggattat catcactttc tggggctact aagccacccg
    15241 tagaactttc gaactctaat atttcaccat ctttatcttt gatatcattt attattttca
    15301 tagcagacat atgatctaat ccagggtcta gtccacattc attgagaaat aataattttt
    15361 ttgatttcac ttcgttatct aaagctttca tttcaggcga aatatatgaa gctgtgaata
    15421 aatgctttga cagagctaaa caatctttgg caataagaat atgatactta gctggcacca
    15481 tagatactat gatatcaaaa ttttttatac attcatatct agcttgatca tctgtaacat
    15541 ctaattttat ttttggtata tctaaatatt ctaataattc taaattttgc tcagcaacag
    15601 taatattcca atttttttct tttgcttgtt ttgatagata atctattaat accttagctg
    15661 aaaaaccagc tcctattact aatattgact tcataattat tgtttttttt gattattatt
    15721 aataaattgt ttcatatatt cataatcggg agtaaacttt cctttataca atatagtgcc
    15781 ccttttgaga ggtaatggaa tttctaattt attaaaattt ttcttccaat ttgcttttaa
    15841 taaagcttct atatatggca agagtatact cgaaaaatat gtactagcat ccattggaag
    15901 ctctgtaggc aagatatcta ctgctagcac tggtaaacct tctgaatata gatctatgat
    15961 ataatcacga gtagcgggat tgtaaattaa tacaggattt tctacttctg tagaagtttt
    16021 tgtgatctct actccaccat taatatcgca tgatatgtcg ccaatgccta ataatctaaa
    16081 atcaggttta ccccaattat caattatata tttctcttct actactcgag gatatttttg
    16141 atcccaataa gaagcatgaa ttaacacatt caaatatggc aaatattgat aaaaaatact
    16201 ttcataattt tcgggatgta aataataatc attgaggtca aatttaccac catcccttct
    16261 ttttaccata tctttttctt taaaaacaac tttgtaaata tgatgtggtg atggaggtat
    16321 atcagaaaat ctcttaagat cctcaggtaa gatttctttt acaggtaaaa aatgtaaaat
    16381 ttcttgtaca cctttactta cattgccata accagtaata cctatgataa atggaatcaa
    16441 actttcaggc aaaccatttt ttaaaatatc aaaaccaatt tctgacatag aagctatgat
    16501 attatcaaga ttagaatatt caaatgctct ttttagctta tcaaaaggat tggcaatacc
    16561 tttataaacc aatcttttac ctaccatcca caatgtatct accaagccag cattacctgc
    16621 ataattgcca aaaaaaatca atcttttacc gaaatcatct actatttttt cgtaatctat
    16681 gagggtggcc ctttgtttga tcagttgttt tagcatagga atattatgtt tttgtccttt
    16741 tatagtatga gaaaagaaaa ggtatacctt atcagcgtat atttcctctt caggtatttc
    16801 ttttatacct attataatat cggcttgttc taaagaattt acaatttttg caccagcttt
    16861 ttcatattct tcatcggtaa aaattctttt atttgaactt tgtactaaaa ctttaaagcc
    16921 ttgctttact agtttattaa catgttctgg gatcacagct actcgttttt cacttggata
    16981 cttggtttca gctcttattc ctaaattcat aattctatat tttatgacaa atatatgaaa
    17041 aataattata ttattaatat attttgtaat attctttatc tgaatagtct tataatatca
    17101 tttaaattag cataaataac taaagctaat aaaaacatca atccaataaa ttgagcacca
    17161 ataagaaatt tttcggaagg tttgcgtcct gtgatcattt ctactaaaac aaaaagaata
    17221 tgtccaccat ctaatccagg tataggaata atattaagga ctgcaagcat aatagataaa
    17281 aaagctgtta gattccaaaa agctaaccaa ttccactgcc ctggaaaaat ttttcccatg
    17341 gttataaagc ctcctaatcc ttcatagcct tttacttctt tagaaaaaat cagacctagt
    17401 tgctttacat aagaattaat aatctcccag gtcctattga aacctctagg tatggattgc
    17461 caaaaagtat atttatacga ataaatttca aaaaattgtg ataagtctgc taccggcatc
    17521 accccaatga tacctgtcga tggtacggcc acatttagag tgatagtgtc gttattcctt
    17581 acgacccata aatcaacttc ttgatctttg tagttttgca ataatgcttt tatatctgtg
    17641 aaataaggtg ttatagtatc ttgtatgcct attattttgt ctcctttttg caatccagct
    17701 aaagctgcgg cactattttc tacaacagaa tcaataataa atggaaaacg agggaaaaaa
    17761 aattgaatag gctcatcctg tttgattagt ttatttaaaa aatctgatgg tacaggtata
    17821 tacacaaggc tatcaccacg ctgtactatg atagtattac ctttttctag taataaagta
    17881 ggtattatct cagtaaatga attgacagga tgatcgtcta tggcaacaac taaatcacca
    17941 ttacgaagtc ctatagcttg aaaagtacta tctactgcta atccatattt caaagatgaa
    18001 ttaggtaaat attgctcccc aatattccat aaaataaaaa tattaataac tattgctaat
    18061 aaaacattca tcaatacccc gccaaacaaa attaaaaaac gttgccaagc cttcttactc
    18121 ctaaattccc atggttgagg ttcatttttc agatggtcgg tatccatgct ctcatctacc
    18181 atgccagcta ttttagtata tcctcctaaa ggtatccatc ctataccata ttctgttcct
    18241 ttataattaa attttaataa actaaattta taatcaaaaa aaatataaaa tttttcaact
    18301 ttgactttaa aaattttagc aaataaaaaa tgaccaaatt catgaactaa aattaaaata
    18361 ctaagagcaa aaagtaattg caaaattttt ataatgactg tcataaatta ttatatttga
    18421 tttataattt cacaagatat ttttcgtgcc ctctgatcta cacttattaa atgttcgacg
    18481 ttttcaacag aagtcggagt gaatttttct aaagtttttt ctatcactgt atacatttga
    18541 gtaaatttga tttctttatt tagaaaggcg taaacagcta tttcattagc tgcatttaat
    18601 acacagggca ttataccacc aatatttaaa atatcatagg ctaactgcag atgagggaaa
    18661 tgtttgatat caggattatc aaatgataga gagtgtagct caacgaaatc aggagtatcg
    18721 aaattagttg gtaggcgata agggtatgta agagcataaa gaataggtat tcgcatatcg
    18781 ggaatactca attgtgcttt aatagatcca tctataaatt gtaccataga atgaataata
    18841 gactcgggat gaatcaaaac cttaatatta gaaggtgcaa taccaaaaag ccaatgagct
    18901 tcgataactt caaacccttt attcatcaga gtagatgaat caatggtaat tttattaccc
    18961 atttgccatc gtggatgcct tagagcttca tcaatagtga tattatcaaa atcttcaaat
    19021 tttagatttt taaatggtcc accagaggct gttaatatta attgactaac agtatttaac
    19081 gcttctcctt gtagacattg aaatatagct gaatgttcac tgtcgatagg tattagaaat
    19141 gctttgcctt gcttcacttc atttatgacc aattcacctg caactactag agcctcttta
    19201 gtagctaaag ctatcctttt acccgcttta gcggcttcaa gagttggttg taagccagca
    19261 aatcctacta aagctattat cactatatca atattatcac tcttagctat atgattgata
    19321 gagtctgcac ctgaaaatac cttgatatca gtgtctttta gcatgtcttt cagttctaaa
    19381 tacaactctt gattactaat tacaacggca tttggatgat gctctaatga ttgcttagct
    19441 aataattcaa cattttcatt agcagttaat acttctattt caaatatttc tggaaacatc
    19501 tcaacaacct cgatagtttg tctacctatt gatccagtgg aacctaaaat agcaagtttt
    19561 attttctctt tcatttccaa aaaatttctg ggtttacagc attgccatcc aaaaaaactg
    19621 agatatctat aaaagatttt tcgggtttgg cattaccaat attagaattg ttttttacct
    19681 gaacgccaat acccacattt actttatcta gatttttgta aatagatatc atattattat
    19741 tatgctgtat aattacttgt ttgccattaa tatgtgatat cataccagta tctatcacgg
    19801 taccatcagc catggctttc actatttctg atgatttact ttctagacgc acattcatag
    19861 gcataggggc ctcaccatat ttttttataa tctttggagt gcttagagga tacgataatt
    19921 taatattatt taaattaaca ttttcagttt tataaaaaaa agcccttaaa gttttattat
    19981 aactagtatc catagtcatt aataaattat gcaaattata ataataaact tctctctgat
    20041 ttaatatctc tactaagcta tcagtttttt tagctaaact ataagccatt tctttttctt
    20101 taatatcacc atatcctgga atatattgcc taagaggtgt ataagcaata attatagtgg
    20161 taactatgat caataacaat acaaataaag ctaaaaaaag aaaaaattta agtctagtaa
    20221 gataaatagt aaaaataacc tctaaattat ctaacctacg taatattagt atataaggat
    20281 gcctaaaatt tctaaataat cttttaggag taatctttac attttttttc acagccaaat
    20341 aaatattttc ttaaaatgca aatttcgtaa aaataaataa aataagtaga attaactttt
    20401 tattttcaat tttttacaaa aaataggaat aataatactt gagcacaatt caatgaactt
    20461 tttaaaatat aaggtgagaa aatgtggtga aaggaatcga aaagggtcca gaagcaaatc
    20521 attgttgtac ttgaagtagt ttatctatac ttcgtattca ccagccgcca atactcatcc
    20581 cacgcccatc tgtccgagga tgcaaggttt aaaagccgac caatgtctga ttctatctca
    20641 cttgttaagt atttatgtgt gattacaaac cacatcagat agctctgcag gtatttagta
    20701 gccactcctc taaatcgccg caaccaaacc ttgaaatcat ttatgtgctt atcgactacc
    20761 tgtacactgt atacaccgcg tctaattatt taattatgct ataaactacg taaagtaaaa
    20821 caaaggatat ttttaagtaa ctccgctcaa taaatcacct ttatgaagcg taatttttac
    20881 aaagctaaat ttcctggcta tgattatttt aattggaata gaaattatag aaggggaaat
    20941 tattgtaatt gtgaaaggaa tcgaaagggg tccagaaaca aatcattgtt ttacttgaag
    21001 tagtttatct atactttgta ttca
//
LOCUS       DAOPRN010000034        20938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_2137_length_20938_cov_158.585692, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000034 DAOPRN010000000
VERSION     DAOPRN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20938
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2137_length_20938_cov_158.585692"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PKI65_05170"
     CDS             <1..977
                     /locus_tag="PKI65_05170"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77864.1"
                     /translation="SKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLKILSAPPLC
                     PDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYIRCMVAGKS
                     LRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGIVEMDELLMNYSEKGRIYKNA
                     EELKRARKQKPKKVAVLAATDRAGNLLFKKFESKKLSSEQIEQFLKAKMPKDGVMCSS
                     NKKAFKKVRRNVIKHKEVVTNHRQKRGIYSLSTVKKKASEFTNWIYFKFRGVATKYLQ
                     NYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNITYVD"
     gene            complement(1394..2134)
                     /gene="kdsB"
                     /locus_tag="PKI65_05175"
     CDS             complement(1394..2134)
                     /gene="kdsB"
                     /locus_tag="PKI65_05175"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOB77865.1"
                     /translation="MNDFWAIIPARYGSTRFPGKPLAIIFDKPMIIHVLDNISKSEIS
                     NIFVATDNEVIYDIVNKHDYNAIMTNIEHKNGSERVNEAANILKIPDDDFIINIQGDE
                     PFISYNLINDLKNFVIDKNINLATAATNFNDFEDLFSPNNIKVVTNKNSKALYFSRSI
                     IPYNRDKNHDEWLNVYPYKKHIGIYVYQKLLLEKLISLNTTLLEQTEQLEQLRWLDHG
                     YEIYVMDTDYQSVSVDTPEDIKKIELLR"
     gene            2689..3687
                     /locus_tag="PKI65_05180"
     CDS             2689..3687
                     /locus_tag="PKI65_05180"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77866.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNVEELKRARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEVVTNHRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNITYVN"
     gene            4072..4662
                     /locus_tag="PKI65_05185"
     CDS             4072..4662
                     /locus_tag="PKI65_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOB77867.1"
                     /translation="MNYEGAKQYILNRLANGLPSNLYYHGLEHTLDVLSAAELFSEME
                     GFTDIDKIYLYTAALFHDAGYLIRYTKNEDASAAIANLVLPRYGYDKESILYINEVIR
                     TTQMPQKPFDRVSAILCDADLDYLGRDDYFVRACNLRKELNEYGIHFSVEDWYEFEID
                     FLKKHNYFTRSAYYLRNEKKLANLELVIKLLEKLKV"
     gene            4689..5543
                     /locus_tag="PKI65_05190"
     CDS             4689..5543
                     /locus_tag="PKI65_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOB77868.1"
                     /translation="MSVILVPFDFTEVANCAVDHAANIAKDLEIPVVLLHIINKDTRE
                     LLNVYKVTNELVNEKLQEKVDEFSNKYGVPFEYEAREGSIFSDIAEASEDHNAFLVVM
                     GTHGRHGIQNLFGSYARRVITSSKIPFIVVQKRLYRPYKKIVTPLDDTLESRQKIKWA
                     IYVSKLFDATIHLKVRSDKIDPIYPVKIRTILSKVEQLFKANDVKYIVDYKEDEDTGS
                     KKFAEQVIEYAHSIDADLILIMTDPDHVNMFNFILKPEDEIMLFNDYEIPVMAINPRD
                     LNFTYVGF"
     gene            5850..7292
                     /gene="pyk"
                     /locus_tag="PKI65_05195"
     CDS             5850..7292
                     /gene="pyk"
                     /locus_tag="PKI65_05195"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOB77869.1"
                     /translation="MNSNFLRTKIIATLGPASWDIETIEKMLLAGTNVFRLNFAHTSY
                     EKADEVVKIVREVSLKNNKPCALLADLQGPKLRIGEIKDGVILENDADVYITNEEILG
                     DANRFTLKYENFTDFREGDQVLIDDGKIVLSIEKVLSAKEMKAKVIRGGPLLSRKGFN
                     VPNANLSIFPITEKDKEDLKFIASHDFDWVALSFIKSAQDILKFRHFLESLESDLSII
                     AKIEKPEALRQLNEIIIAADGVMIARGDLGVEMPIYQLPVWQKRIITRARQLAKPVIV
                     ATQLLRSMIDDIIPSRAEVTDIANAVLDGADALLLTDETGIGKHPVLVVETMAKVIGE
                     VEKNPEFTKFLTKNFKMASPLKLNCNRIQYVTASIATEVVLRYPISAIIGLTYTGSSI
                     IQMSSYRSPVPIYAFTSQEKVIRKLCLIWGVAPFNYDNQNSTDESIEDTLNYLKENNF
                     LRVGDWVIHTASMPIKASKHINTVKISIVE"
     gene            complement(7534..9921)
                     /locus_tag="PKI65_05200"
     CDS             complement(7534..9921)
                     /locus_tag="PKI65_05200"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOB77870.1"
                     /translation="MKKVISVCSLFIIPVIASASEASLVVPESMKSVNWLYWGFLITI
                     LGLIFSFWQYKKVVKQRAHESMLDVANVIYQTGKTYLIQQGKFLIILFLIVGSVIAFY
                     FGYLSEPRFSFGNVLFILMWTLIGILGSYSVAWFGIRVNTLANSRMAFASLENKPFKL
                     FNIPMQAGMSIGMMLISLELMIMLFILMVLPGELAGASFIGFAIGESLGASVLRIAGG
                     IFTKIADIGSDLMKVIFKIGEDDPRNPGTIADCTGDNAGDSVGPTADGFETYGVTGVA
                     LITFILLAVDGINNQVQFLAWIFGIRLTMIIASLLGYYINNAISNKTFGKSKEVDFEK
                     PLMSLIVITSIISIAMTFLVSYLILGANYPRLWVGLSIIVSLGTLAALIIPELTKFSV
                     SPKSKHTKVVMESSKRGGASLNILSGIVSGYYSAFYHGLAIAILMFLGYLTTQSFGLS
                     AYMIYPVVFSFGLIAFGMLGMGPVTIAVDSYGPVTDNAQSIYELSLIEEIPNIEQEIE
                     KDFNFKPNFDDAKHYLESNDGAGNTFKATAKPVLIGTAVVGATTMIFSIILVIKNTLG
                     IDPAQILNILNPFTILGFLLGGSVIYWFSGAANHAVNIGAGKAVNYIKQHIQLDPNAP
                     KKASTKTSKEVVRICTIYAQNGMINIFIAIFSFALAFAFFSSPAYDINAVSFFISYLI
                     SIAFFGLMQAVFMANAGAAWDNAKKIVEVDMKQKGTPLHDATIIGDTVGDPFKDTASV
                     SLNPIIKFTTLFGLLAMEIAISDGFKYIAPYIGIVFLIIALFFALRSFFGLKVTN"
     gene            10178..11404
                     /gene="rocD"
                     /locus_tag="PKI65_05205"
     CDS             10178..11404
                     /gene="rocD"
                     /locus_tag="PKI65_05205"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOB77871.1"
                     /translation="MEKYSSKQLIEIDSRYGAHNYHPLPVVLERGEGIYVWDVEGKKY
                     YDFLSAYSAVNQGHCNPRIVNAMIEQVKTLTLTSRAFYSKALCLFTQYITNLFGYDKV
                     LMMNSGAEAVETSLKLARKWAYEVKGISQYDAKIITCNNNFHGRTITIISFSTDPDAY
                     TGYGPFTPGFVQIPYNDVQALEEALKDPNVVAFLVEPIQGEAGVVVPDEGYLKKCYDL
                     CKKNNVLFIADEIQTGLARTGKMLACDYEDVRPDILVLGKALSGGMMPISAVLADDEI
                     MLTIKPGQHGSTFGGNPLACIVAKEALEVIVDDKLADNAGKLGEIFRTELKKLQKEFD
                     VLEIVRGKGLLNAIVIKPKNGKEAWDLCLKMAEYGLLAKPTHQHIIRLAPPLIINENQ
                     ILESIDIIKRALKDTF"
     gene            11516..11950
                     /locus_tag="PKI65_05210"
     CDS             11516..11950
                     /locus_tag="PKI65_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOB77872.1"
                     /translation="MLGLNKVCLIGYVGRDPEVKVDNPDNKKVSFSLAVTTMRRENTD
                     NTEWFNIAAWGRLAEIAEKYVRKGMPLYIEGRFRSYEFTDNLNMQRRGYEIVADNLIM
                     LSNKDDKFPADNIQNEQQLGATPTNVDLEIEESFEPSDDLPF"
     gene            11979..13292
                     /locus_tag="PKI65_05215"
     CDS             11979..13292
                     /locus_tag="PKI65_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOB77873.1"
                     /translation="MEDIPLISTGLFVILFVLSTLMYIIVALLEYTFFQLPINFIENN
                     QKPKNAIEFYLKNYLEKPEAPLFTWFVFRIFIIIFQTFLLWLFILNHVTLINIIVALL
                     ISFVCVGIVDYIFGEWFLLLIIKSLKPKTIIKYFLWFFIPFIAINKQLLIIFPVFKKI
                     FSDRFAQKENLTFDELSDAIESTLSTAHSDDSNTTTVLLKGVIKLSDIEVKEIMKPRI
                     DVIAIPIESTFKEIIDLILEYEYSRYPVYEKDIDNIKGVLYIKDLLPFMTNAKDDFCW
                     QKHIRSPFFIPENMLASNLLKEFQKNRVHIAIVVDEYGGTSGIVTLEDLLEEIVGEIQ
                     DEHDNDSDEIFTKKIDENTFLVDARISLNDFVKLIQCDEDYFEEFEDQIETIAGVILS
                     LLGNFPQKNQEVIYKNCKFKILSLDERRIKIVRVELLPEKEEDDD"
     gene            13282..13884
                     /locus_tag="PKI65_05220"
     CDS             13282..13884
                     /locus_tag="PKI65_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOB77874.1"
                     /translation="MMIEIKKIFVLYLFVILFFTSCLKEETMPMPKPFGYMRIALPEK
                     EYKWLDTVYPFKFSYPAYAILTPYHGKSNEENAKYWFNITIPRFNAYIYFTYKEIQNN
                     LFDLKEDSRTFVVKHISKSSNIENILINKPEKKVYGVIYEIEGNDVASPLQFFLTDST
                     KHYLRGALYFNNIPNNDSLKPIIDFLKIDIDTLINTFSWK"
     gene            13950..14804
                     /locus_tag="PKI65_05225"
     CDS             13950..14804
                     /locus_tag="PKI65_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77875.1"
                     /translation="MKKIVLFLVINFSVFISILAQEIKLATPEQFKKFLNTTTYVVMS
                     DNPFSEFNNVITESINKVWKLTPYKFIDNEEFDKLKINPNNSFIFLSEASFTLNNETI
                     YVDILNITLGVRSGNLNLMPDLGSAPLAYLPEGADSDEIEEQYLYFIPGILRFFQYNI
                     KFQANQTKVVDLNKLASSNKNLLTNKTIYFCQENLSNEVNNLANISKIYTGKVKITNK
                     DEIYKLLLDGDPNAAVAFTVKTNGKTGLYCIKFIIDADSGTLIYYDMHLVKNKDNDGF
                     TANDFKKL"
     gene            15341..16849
                     /locus_tag="PKI65_05230"
     CDS             15341..16849
                     /locus_tag="PKI65_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOB77876.1"
                     /translation="MTKNSVSSLAKQTVIYGASSILGRLLNYLLVPLYSRLFLPVEAA
                     VYIEFYAYAAIFIVILTYGMETAFFRFINDQNIKDKDRVYSTSFSSILLTSSLFVIIL
                     SIFSNGIATSLGYPGNAEYVIFFAIVAGADAVASIPFAYLRSKGKAMTFASIRIINIV
                     VNVFLNILFLVIIPNAYNNHPELQSFLSIFTDGKPWVGYIFVANVVTSLLNLILLFPH
                     YKQVKIKIDKKLWNKMLKYAIPLLILGLSGILNNNIDRILLKNLLPPEIALHQMGVYG
                     MVFKFSVIMSLAIQAFRYAADPFFFEQSKDSDAKGVYAKVMNYFVITLVIIYAFTMLN
                     MDYIKYFVGKAYFEGLTVVNILLLSQLFLGIFYNLSIWYKLTDKTIMGAIVSIIGTTI
                     LIILNIILIPKIGYVGSAWANLICNFSIVLISYFLSQKYYPVPYELKKIIVYIITGII
                     IVYIGSFVPTIDNHFLNILAHNSLILLYIPIIYYLEPTFWLFIKNTFKRKKH"
     gene            complement(16891..18396)
                     /locus_tag="PKI65_05235"
     CDS             complement(16891..18396)
                     /locus_tag="PKI65_05235"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HOB77877.1"
                     /translation="MKKFFAIVILSFLLINISLAQKTPAYKIQVIIPQLADTNVYLGY
                     YYGKWQYVRDSIKLDKNGRGTFHTNDTVGSGMYLLITPSKKYLEFILNDNERIFTIET
                     DTTDLLGNAKVKGSKENELFFQFNKTISKPSKEIYQLQQKVKDFKQKNLNDSVKYYND
                     KIKNLIDSINTMKLKYMEQYPNTLLARIFKATKEPEIPDTLPLTTDGKPDSTYQYYYY
                     KEHYWDNIDLADEALLRTPIYHNKLEFYFNNVIVQHPDSIIKEIDKVLAKASKNKETF
                     KYVLWWLTYNYETSKVMGFDKIFVYLADNYYKTGKAFWLPETSVQRILDRANEIRPTL
                     IGTIATNLILLDTAKNLISLHNINAKYTLLYFWDPTCGHCKKETPKLVDFYNKYKDTF
                     NLQVFAVCTDSSFNEMKKAIKTYNMYDFINVNGIYTASVDFRKYYDIYSTPVLYVLDQ
                     YKRIIAKRLSVDQLYDFLKNYEKNPLFPDPPEPMQWKSIPINPQSPVEQNY"
     gene            18583..20361
                     /locus_tag="PKI65_05240"
     CDS             18583..20361
                     /locus_tag="PKI65_05240"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77878.1"
                     /translation="MNKNIFFLLLFCFALSDLVFSQSEDTITIKHVEAYEWNFKNYLI
                     HPDSLRYDFDTVPFFHFYPYKDYKYNLTNDLVGGIVYPARIPDLIKHQKISNIRLPYL
                     KFVGFFDPIDIPLIKSDTAIMQARFMQGIKREEFFQTHFTMPILDFANFNFFYRTFYA
                     PSTYANSMVRQNQFYINFFGNTKNKKYNYYLGLIQQNRTTKENGGIKNMSSFVDSAKT
                     DRILLNIYLPEASAYHKINDYYYSHSYKINKFIELFHYISYSQYSYRYTDNKPSEDFY
                     YLYDGLQNDSIFDSLNVWLLDNIVGVKANTKNWDFNINYNYSFGKFKMNLLKSKPIFT
                     FINADINFKKFVNISMQYYWGDYRDQKVYISGHFPSFSYGIGYNKFSQPYIYSHFLGK
                     YYRYDKLLPETEIIWANLKNKNKFGNVALCGGYVKKQLFFDKYGFIRSSDPYLFATLE
                     FDLRFDFGKHFVLQTYHLFQYPYNKKTISVPVWLTRNVFYYHTPLFKGNAHLKTGIEF
                     LYLTKYYAPAWNPNIQYFVVSENKLGNFIYPSLNIQLKVKKAIIFVQLENFTDGVFKT
                     RQYMTIAGHPMRDMTFRWGLQWNFYN"
     gene            complement(20441..20608)
                     /locus_tag="PKI65_05245"
     CDS             complement(20441..20608)
                     /locus_tag="PKI65_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOB77879.1"
                     /translation="MAYVITEDCTACGSCIDECPAEAISPGDIYKIDPELCTDCGSCA
                     EVCPVEAIHPE"
BASE COUNT         7741 a         2809 c         3108 g         7280 t
ORIGIN      
        1 ccagcaaata cctcgctatg agtaaggaag agaataaaga attcttagac aacctttatt
       61 cattaatgaa aaaattagaa gccgatgtag tcgattatca aggcctaaaa atactcagtg
      121 ccccaccact atgcccagac tgtcgaagca atcacatcat taaaaatggc ttacaaaatg
      181 ggatgcaaaa ctatcgttgc aaagattgcg gcagacaatt tagagttacc actggaactt
      241 ttgtttacag gttgcaaaag aaagagaaaa tgctcgatta catcagatgt atggtagcag
      301 gcaaaagtct cagagcttgt gctaaagaag tgggcatcag cctacctaca agcttcgcat
      361 ggcgacacaa gatattagca gctctacaaa gtttcgaaga taatatcaat tttttcggta
      421 tagtagagat ggatgaacta ttgatgaatt attccgaaaa aggtagaata tacaaaaatg
      481 ccgaagagtt gaagcgagct aggaagcaaa agccaaagaa agtagccgta ttggcagcta
      541 ctgacagagc tggcaactta ttattcaaaa aatttgaaag caaaaaacta agttcagaac
      601 agatagaaca atttctgaag gctaaaatgc caaaggatgg agtcatgtgt agcagtaata
      661 aaaaagcatt caaaaaggtt agaaggaatg taatcaagca taaagaagta gtaactaacc
      721 atagacaaaa gcgaggtata tatagcctaa gtactgtcaa gaaaaaggct agtgaattca
      781 cgaattggat atatttcaaa tttaggggtg tagctaccaa atacctgcaa aactacatta
      841 tgtggtatgt agtgatgggt aaatacctca caggcaagac tgtaagcaat actgggcgaa
      901 tgctcaacct cgcatcctcg gacaggtggg catggtatcg gtatagggag ttagtaaata
      961 taacatatgt tgattgaaac atatattagt tttccaatta tatcctcaac ttcccaaaat
     1021 tttaaaaatg catctccaag ccttataatc taattattta tgaaaattta tataagaata
     1081 aataataaac attgagaaat aaagaaaagt ttaaattatt aattgcagca taaataggaa
     1141 tatagcctaa attcactcta aaaattatcg ataaaacaaa ataataagta gattaaatta
     1201 attattatat cattaaactt gaaattacat tataataagc aaagcagtat atttattttt
     1261 tagaatttat taaaacctat aatgattatt taagaaaatc agataaatac aggattaata
     1321 aaaaatttat gttaacaaaa aaattaactt tcatttgttt aataaaatat atttagatca
     1381 attcaagtgt ttattatcta agaagttcta tttttttgat atcttctggc gtatctacac
     1441 tcacactttg gtaatctgta tccatcacat atatttcata tccatgatct agccaacgta
     1501 attgttctaa ttgttcagtt tgctctaata atgtagtatt taatgatatt aatttttcga
     1561 gtaataattt ttgataaacg taaataccta tatgcttttt gtatggatac acattgagcc
     1621 attcatcatg atttttatct ctattatatg gtattattga acgactaaaa tataaagctt
     1681 tgctattctt atttgtaaca acttttatat tatttggtga aaataaatcc tcgaaatcat
     1741 tgaagtttgt agcagcagta gccaaattaa tattcttatc tatcacaaaa ttctttaaat
     1801 cattaattaa attataggaa ataaaaggct catcaccctg tatgttgata ataaaatcat
     1861 catctggaat ttttaaaata tttgcagctt catttactcg ttcactacca tttttatgtt
     1921 caatatttgt cattattgca ttataatcat gtttatttac tatatcatat ataacctcat
     1981 tatctgtagc tacaaaaata ttagaaattt cactcttaga tatgttatct aaaacatgaa
     2041 taatcattgg tttatcaaaa attattgcta gtggtttccc tggaaaacga gtgctgccat
     2101 atctcgcagg tattattgcc caaaaatcgt tcatgattaa ttattttaat acaaagttaa
     2161 attaataaaa tgagtccatt tagaaaaatt attaaaaaat gcaaaaataa agctgagtta
     2221 aaaagacaaa aataagtttt aataaaaata actcataaaa ttaaaataat ttcacataaa
     2281 ttggtaaaaa tagtttttac ttagcaaaaa ataaatagta aataaatata tataaaaagg
     2341 gataatacat aaaaaatagc agaattcact tgaggattat aataattttt tggttggtaa
     2401 agcgaaagat gagtgagaag gcacgaatga ttagcataat aaatttagaa aaagagtcat
     2461 tctacaatag atgagatcat atgtcaatta aaatatatat taagaagtgg taaatcaaca
     2521 tatgagggta agtttaagca aatgatatga gcaacattaa gagtaatata gataaatctt
     2581 aagaggataa ttgaatattt aatagatgga aaaataaatt aataaatgtt taagtttttc
     2641 aagtttatat gttaaataat ttttatattt ttacaaaaat tttaattcat gaaaaaaatt
     2701 gtagaaaaca ccagtaaata ccttgctatg agtaaggaag agaataaaga attcttagac
     2761 aatctttatt ctctaatgaa aaaattagaa gccgatgtag tcgattatca aggcctgaaa
     2821 atactcagtg ccccaccact atgcccagac tgtcgaagca atcacatcat taaaaatggt
     2881 ttacaaaatg ggatgcaaaa ctatcgttgc aaagactgcg gcagacagtt tagagttacc
     2941 acaggtactt ttgtttacag gttgcaaaag aaagaaaaaa tgctcgacta catcagatgt
     3001 atggtagcag gcaaaagtct cagagcttgt gctaaagagg tgggcatcag cctaccaaca
     3061 agcttcgctt ggcgacacaa gatattggca gctctacaaa gtttcgaaga taatatcaat
     3121 tttttcggcg tagtagagat ggacgaacta ttgatggatt attccgaaaa aggcaggata
     3181 tacaaaaatg tcgaagagct gaagcgagcc aggaagcaaa agccaaagaa agtagccgta
     3241 ttggctgcta ctgacagagc tggcaactta ttattcaaaa aatttgatgg caaaaagcta
     3301 agttcagaac aaatagagca atttctgaag gctaaaatgc caaaggatgg tgtcatgtgt
     3361 agcagtaata aaaaagcatt caaaaaggtt agaaggaatg taatcaagca taaagaagta
     3421 gtaactaacc atagacaaaa gcgaggtata tatagcctaa gtactgtcaa gaaaaaggct
     3481 agtgaattca cgaattggat atatttcaaa tttaggggcg tagctaccaa atacctgcaa
     3541 aactacatta tgtggtatgt agtgatgggt aaatacctca caggcaagac tgtaagcaat
     3601 actgggcgaa tgctcaatct tgcatcctcg gacaggtggg catggtatcg gtatagagag
     3661 ttagtgaata taacatatgt taattgaaac atatattaaa attctacaca cttccaaatc
     3721 tacatctcca aatttacata ttaacaacag tttcaagttt tcaacgttaa ttcaacatta
     3781 ttgaattaag aaattttatg aataatctaa tattcataat cttggtaatc actaataaaa
     3841 ttatataatt ataaaaaata aaattcaagt ggataaaagc caattactaa tatcttataa
     3901 cctggtataa atactttttt taatcaaaat aatttaaaag aaggggttac attttattta
     3961 taaaattgta taaattagaa tacacttgat ttacacaatg aaaatttgta actattctta
     4021 tattttaatt ttttttaatt atctttgcca tattattttt ttaaaaaata aatgaattac
     4081 gaaggggcta aacaatacat actaaataga ttagctaatg gattaccatc taatttatac
     4141 tatcatggtt tagaacatac tttagatgta ttatctgctg cagaactttt tagtgagatg
     4201 gaagggttta cagatattga taagatttat ttatatacag ctgctttgtt tcatgatgct
     4261 ggttatttga tacgttatac taaaaatgag gatgcatcgg cagcgattgc caacttagtt
     4321 ttaccaagat atggatatga caaagaatct attctttaca taaatgaagt aataagaact
     4381 acacaaatgc cacaaaagcc atttgataga gtaagtgcta tattatgcga tgcggattta
     4441 gattatttag gaagggatga ttattttgta agagcttgta atttaagaaa agaattaaat
     4501 gagtatggta tacattttag tgtagaagat tggtatgaat ttgaaataga ttttttaaaa
     4561 aaacataatt attttacaag atcagcatat tatttaagaa atgaaaaaaa attagccaat
     4621 ttagaattgg taattaaact tttagaaaaa ttaaaggtct aactaataga taataaaaaa
     4681 taaaatatat gagtgtaata ttagttccat ttgacttcac agaagtagcc aattgtgccg
     4741 tagaccatgc ggctaatata gctaaagatt tagaaattcc tgtagttttg ttgcatatta
     4801 ttaacaaaga tactcgagag ctattaaatg tttataaagt aactaatgag cttgtaaatg
     4861 aaaaattaca agaaaaagtt gatgaatttt caaataaata tggtgttcct ttcgaatatg
     4921 aagctagaga aggtagtata tttagtgata ttgcggaagc tagtgaagat cataatgctt
     4981 ttttggtcgt tatgggaacg cacggtagac atggtataca aaatttattt ggtagctatg
     5041 ctcgtagagt tattactagt agtaagatac cttttattgt cgtccagaaa aggttatata
     5101 gaccttataa aaaaatcgtt actcctctag atgatacatt agaatctcgt caaaaaatta
     5161 aatgggccat atatgtaagt aaattattcg atgctacgat tcatttaaaa gttcgttctg
     5221 acaaaattga cccaatttat ccagtaaaaa taaggactat actttctaaa gtagaacaat
     5281 tatttaaagc taatgatgta aaatacattg tagattataa agaggatgaa gatacaggct
     5341 caaaaaaatt tgctgaacaa gtaatcgaat atgctcatag catagatgca gaccttattc
     5401 tgattatgac agatcctgat cacgtaaata tgtttaattt tattcttaaa cctgaagatg
     5461 aaatcatgct tttcaatgat tatgaaattc ctgttatggc tatcaatcca cgtgatctta
     5521 attttactta tgtagggttt taataattta caatatacta ttgagaaatt ttaaatctgt
     5581 aatttttctc tttgtgaaat aactaagtaa atattgctcc agtaatattt atttttatag
     5641 tataataaaa ttaattaata aatttttaat attatttatt tccgcagtct cagattctca
     5701 tattcacaac ttttacttgg tatctcaaag tatttataat tattatttat taaaaaataa
     5761 attaggattt caataaatgt aaatcaaaag ttttattatc gaattaattt attttattat
     5821 tatacttaac tttgtaaaaa tattaaatta tgaattcaaa ttttttgcgt acaaaaataa
     5881 tagcaacgtt gggacctgca tcttgggata tagaaactat agaaaaaatg cttttagctg
     5941 gtacaaacgt ttttagatta aattttgcac atactagtta tgaaaaagct gacgaagttg
     6001 ttaagattgt cagagaagta tcacttaaaa ataataaacc atgcgcattg cttgcagatt
     6061 tgcaaggacc taagctgaga attggtgaga taaaagatgg agtgatatta gaaaatgatg
     6121 ctgacgtcta tattacaaat gaagaaatat taggagatgc gaatagattt actctaaaat
     6181 atgaaaattt tactgatttt agagaaggtg atcaggtatt aatagatgat ggcaaaatag
     6241 tactaagtat agaaaaagta ttaagtgcta aagaaatgaa agctaaagta ataagaggtg
     6301 gtccattgct ttctcgaaaa ggctttaatg tacccaacgc taatctatct atatttccta
     6361 ttacagaaaa agataaagaa gatttgaaat ttattgcttc tcacgatttc gactgggtgg
     6421 ctttatcttt tataaaaagt gctcaggata tattaaaatt tagacatttt ttagaatcac
     6481 tagaaagtga tctcagtata attgccaaaa tagaaaaacc cgaagctttg agacaattaa
     6541 atgaaataat aatagctgct gatggagtaa tgatagccag aggtgactta ggtgtagaaa
     6601 tgcctatata tcaacttcct gtttggcaaa aacgaattat cacaagggca cgacaattag
     6661 ctaaaccagt tatagtagct actcaattat taagaagtat gatagatgat attatacctt
     6721 cacgtgcaga agttacagat atagccaatg cagttttaga tggtgcagat gcattattac
     6781 taacagatga gacaggaata gggaaacatc ctgtcttagt ggtagaaaca atggcaaaag
     6841 taataggaga agttgaaaaa aatcctgaat ttactaaatt tttaactaaa aatttcaaaa
     6901 tggcctctcc attaaaatta aattgtaata gaatacaata tgtaacagct tccatagcga
     6961 ccgaagtagt gctgagatat cctatctctg ctattattgg tctcacttat acgggatcat
     7021 ctattataca aatgtcatct tatcgatcgc ccgtgcctat ttatgcattt acatcgcaag
     7081 agaaagttat acgtaagctc tgtcttattt ggggagttgc cccatttaat tacgacaatc
     7141 aaaatagtac agatgaaagt atagaagaca cattgaatta tttaaaagaa aacaattttt
     7201 tacgcgttgg tgattgggtg atacacactg ctagcatgcc tataaaagct agtaaacaca
     7261 taaacactgt aaaaatatcc atcgtagaat aaggaaatat catattacaa tttacaattt
     7321 aattaactaa aattaaagaa ttttaattaa gtatttaaaa atataatagc tacacttttt
     7381 tgtttcacta ttcagctatc atatgtgtta aaatttcctt cttataaaag aattagattt
     7441 gtcaaaattt attacctcta acaatgatct tatgctgata agtattttat atattcatat
     7501 tctttaacta taataatgtt agaatttgcg tatttaatta gtcactttta atccaaaaaa
     7561 tgatctaaga gcaaaaaata aagctattat taaaaatact atgccaatat atggagcaat
     7621 gtatttaaaa ccatcactaa tagcgatttc cattgctaat agtccaaaca aagtagtaaa
     7681 tttaataata ggatttaaag acactgaagc agtatcttta aaaggatcac caacagtatc
     7741 gcctattatg gtagcatcgt gtagaggtgt tcctttttgt ttcatatcta cttctacaat
     7801 tttttttgca ttgtcccaag cagcccccgc attagccata aatacagcct gcataaggcc
     7861 aaaaaaggca atagaaatta gatagctaat aaaaaaagaa actgcattaa tatcgtaggc
     7921 aggtgaagaa aaaaatgcaa aagctaaagc aaatgaaaag atagctataa aaatgtttat
     7981 cattccattc tgtgcataga tagtacagat acgtacgact tcttttgatg ttttagtaga
     8041 tgctttttta ggagcattag gatctaattg tatatgttgt ttaatataat ttactgcttt
     8101 gccagcacct atatttactg catgattagc cgctccacta aaccaataaa tcacactacc
     8161 acctaataaa aatcctaaaa ttgtaaatgg atttaatatg ttcaatattt gtgctggatc
     8221 tatacctaat gtatttttta taactaaaat aatagaaaaa atcatagtag tagcacctac
     8281 tactgcagta cctatcaaaa ctggtttggc agtagcctta aaagtattac ctgctccatc
     8341 attagattct aaataatgtt tagcgtcatc aaaatttggt ttaaaattaa aatctttttc
     8401 tatttcttgt tctatatttg ggatttcttc gattagtgat aattcataga tagattgagc
     8461 attatcagta acaggaccat aactgtcgac agctatggtt acaggtccca tccctaacat
     8521 accaaaagct attaatccaa agctaaatac tactggatag atcatatatg cagataatcc
     8581 aaatgattga gtagttagat atccgagaaa catcaaaata gctatggcta aaccatgata
     8641 aaaagctgaa taataaccag atacaatacc agataaaata tttaaagatg cgccaccacg
     8701 cttgctagat tccataacta ctttggtatg tttagatttg ggactaacag aaaattttgt
     8761 taattcagga attattagtg ctgctagtgt tcctaaactt actataatac ttaatcccac
     8821 ccataaacgt ggataatttg ctcctaaaat taaataactt actagaaatg tcatagcaat
     8881 agaaataata gatgtaataa caatgagcga cataagtggt ttttcgaaat ctacttcttt
     8941 agattttcca aaagttttgt tagatatagc attattaata taataaccta ataaagaagc
     9001 tattatcata gtaagacgaa taccaaaaat ccaagctaaa aattgcactt gattattaat
     9061 gccatcaact gctaataaga taaatgtgat aagagctaca ccagttacac cataagtttc
     9121 gaatccatca gcagttgggc ccacactatc tccagcatta tcacctgtac aatcagctat
     9181 agtaccagga ttacgtgggt catcttcacc gattttaaat attactttca taagatcaga
     9241 gccaatatca gcaatttttg taaaaatccc tcctgctatt cttaaaactg atgcacctaa
     9301 agattcacca atagcaaaac ctataaaact tgcacctgct aattctccag gtaataccat
     9361 caatataaat aacattatca tcaattctaa gcttattagc atcattccta tactcatacc
     9421 agcttgcata ggtatattaa atagtttaaa aggtttattc tctaaacttg caaaagccat
     9481 tcgagagttt gccaatgtat ttaccctgat accaaaccag gctacagaat atgatcctaa
     9541 aatacctatt agagtccaca tcaatataaa taaaacatta ccaaagctaa acctaggttc
     9601 actcaaataa ccaaaataaa aggctattac agaacctact attaaaaata atataattag
     9661 aaattttcct tgttgaatta aatatgtttt gccagtttga taaattacat ttgctacatc
     9721 gagcatgctt tcgtgcgctc gttgtttgac tactttttta tattgccaaa aactaaatat
     9781 tagaccaagg attgttatca aaaagcccca gtataaccaa ttgacagatt tcatgctctc
     9841 aggaacaact aagctagcct cactagcact tgctattaca ggaatgataa aaagagagca
     9901 aacactaatt acttttttca tatttaagtt tttcttgcaa atttataaat attttttcat
     9961 cttcaatcat cttgaattat taagatttgg ttttttaata ttatgatagt aatgtaatta
    10021 ttgtttatct ggaataatat tttatttatc aaaaatatta taaattattt gttataaatt
    10081 atttattatc tcatttttat tttataaatt attataaatt ttgatttatt gtaactcaga
    10141 caattaaatt tttattatat ttgtaaaaaa gtattttatg gaaaagtata gttcaaaaca
    10201 attgatcgaa attgattcga ggtacggtgc tcataattat cacccactac cagtagtgct
    10261 agagagaggc gagggcatat atgtatggga tgtagaaggt aaaaagtact atgatttttt
    10321 atcagcttat agtgcagtaa atcaaggaca ttgcaatccc agaatagtaa atgctatgat
    10381 agaacaagtc aaaaccctta cattgacttc tcgtgctttt tattctaaag ctttgtgctt
    10441 gtttactcaa tacataacaa atctatttgg ttatgataaa gtattaatga tgaattctgg
    10501 agccgaagct gtagaaacat ctctcaagtt agctcgtaaa tgggcttatg aagtgaaagg
    10561 aatatcacaa tacgatgcta aaataattac ttgtaataat aattttcatg gacgtactat
    10621 tactattatt agcttttcca cagatccaga tgcatatact ggatatggtc catttacccc
    10681 aggttttgta cagatacctt ataatgatgt acaagcccta gaagaagccc ttaaagatcc
    10741 taatgtagtt gcttttctag tagaacctat tcaaggtgaa gctggtgtag tagtacctga
    10801 tgagggttat ttgaaaaaat gttacgattt atgcaaaaaa aataatgttt tatttatcgc
    10861 tgatgagata cagacaggat tagcccgtac aggtaaaatg cttgcatgtg attatgaaga
    10921 tgtacgccca gatattttgg tcttaggtaa agctttgagt ggtggtatga tgcctatatc
    10981 agctgtatta gctgatgatg aaatcatgct tactattaaa cctggtcaac acggttctac
    11041 ctttggtggc aatcctttag cttgtatagt tgctaaagaa gcattagaag taatagtcga
    11101 cgacaaatta gcagataatg caggcaaatt aggtgaaata tttagaactg aactgaaaaa
    11161 attacaaaaa gaatttgatg ttttagaaat agtaagaggt aaaggtctat taaatgccat
    11221 agttataaaa cctaaaaatg gtaaagaagc atgggatcta tgcctcaaaa tggcagaata
    11281 tggcttatta gctaaaccaa cacatcagca tattataagg cttgccccac ctcttataat
    11341 taatgaaaat caaatattag aatctataga tattattaaa agagccttaa aagatacatt
    11401 ttgatttctt tataaaggtg gattttaaat ttaaaatcca cctttatttt tttttaaaat
    11461 aaatctacca tataaaatat tattattaat tttgaaaaaa taaaatatgt aaaatatgtt
    11521 aggtttaaat aaagtttgtt taataggata cgtaggtaga gatccagaag taaaggtaga
    11581 taatccagac aataaaaaag tatcttttag cttagcagtc actactatga gacgagaaaa
    11641 tactgataat actgaatggt ttaatatcgc agcttggggt agactagctg aaatagcaga
    11701 aaaatacgta agaaaaggaa tgcctttata tatagaaggt agatttcgct cttatgaatt
    11761 tacagataat ctaaatatgc aaagacgtgg ttatgagata gtagctgata atctaataat
    11821 gctatcaaat aaagatgata aatttcccgc tgataatatt caaaatgagc aacaactcgg
    11881 agctacacct actaatgtag atttagaaat cgaagaaagt ttcgaaccta gtgatgactt
    11941 gcctttttaa aaaataatgg agtagtagtc tataaaaaat ggaagatatt cccctaattt
    12001 ctacaggttt atttgtaata ttatttgttt tatctacatt aatgtatatt attgtagctt
    12061 tactagaata tacatttttt caattgccta ttaattttat tgaaaataat caaaaaccta
    12121 aaaatgcaat tgagttttat ttaaaaaatt atttagaaaa acctgaagca cctttattta
    12181 cttggtttgt atttcgcatc tttattataa tatttcaaac tttccttttg tggttattca
    12241 ttctaaatca tgtaacccta ataaatataa ttgttgcctt actgatttca tttgtgtgtg
    12301 tgggtattgt tgattatatt tttggagaat ggtttttgct tttaattatt aaatcattaa
    12361 aacctaaaac aataatcaaa tattttctgt ggttttttat accttttatt gctataaata
    12421 agcaattatt aatcatattc ccagttttta aaaaaatatt ttcagacaga tttgcacaaa
    12481 aagaaaattt aacttttgat gagctatcgg atgctataga atctactcta agcactgctc
    12541 atagtgatga ttctaatact actactgtac ttttgaaagg agtcataaaa ctaagcgata
    12601 tagaagtaaa agaaataatg aaaccacgta tagatgtaat agctatacca attgaatcaa
    12661 cattcaagga gattattgat ctgatattag agtatgagta ttctcgttac cctgtatacg
    12721 aaaaggacat agataacata aaaggcgtat tatatataaa agatttgtta ccttttatga
    12781 ctaatgctaa agatgatttt tgttggcaaa aacatattcg tagtcctttt tttatcccag
    12841 aaaacatgct tgcaagcaat ttattgaaag aatttcaaaa aaatcgagtt catattgcta
    12901 tagtggtaga tgaatatggt ggcacctctg gtatagtgac attagaagat ttattagaag
    12961 agatcgtagg agagatacaa gatgaacacg ataatgactc tgatgagata tttactaaaa
    13021 aaatagatga gaatacattc ctagtagatg ctcgtattag cttaaatgat tttgttaaat
    13081 taatacaatg tgatgaagat tatttcgaag agtttgaaga tcagatagaa acaatagcgg
    13141 gtgttatttt atcattgctt ggtaatttcc ctcaaaaaaa tcaggaagta atttataaaa
    13201 attgtaaatt taaaatttta tctttagatg agcgtagaat taaaatagta cgtgtagagc
    13261 tattacccga aaaagaagaa gatgatgatt gaaataaaaa aaatttttgt attatattta
    13321 tttgtaattc ttttttttac tagttgctta aaagaagaaa ccatgcctat gcctaaacct
    13381 tttggttata tgagaatagc tttacccgaa aaagaatata aatggttaga tacggtctat
    13441 ccttttaaat ttagttaccc tgcgtatgct atattgaccc cttatcatgg aaaaagcaat
    13501 gaagaaaatg caaaatattg gtttaatatc acaataccta gattcaatgc ttatatttat
    13561 tttacttaca aagaaataca aaataattta tttgatctca aagaagactc acgcactttt
    13621 gtagtgaaac atatttctaa aagctctaat atagaaaaca tactgataaa taaacctgaa
    13681 aaaaaagttt atggagtgat atatgagata gaaggtaatg acgtagcttc accgttacaa
    13741 ttttttttaa cagattcgac caaacattac ttgagaggtg ctttgtattt taataatata
    13801 cctaataatg attctttgaa acctataata gatttcttga aaatagatat cgatacactg
    13861 ataaatacat ttagttggaa ataaatatat gcaaaatatt taattataat tcctattttt
    13921 gctttaaaat tatttaaaag tatataaaaa tgaaaaaaat cgttctattt ttagtcataa
    13981 atttttcagt ttttattagt attttagctc aagaaataaa actagctact cctgaacaat
    14041 ttaaaaaatt tctcaacact actacctatg tagtgatgag tgacaatcct tttagtgagt
    14101 tcaataatgt tattacagaa tctatcaata aggtttggaa actaacacct tataaattta
    14161 tagataatga agagttcgat aaattaaaaa taaatccgaa taattcattt atttttttgt
    14221 ccgaagctag tttcacattg aataatgaaa ctatctatgt agatattttg aatataactc
    14281 tcggcgttag atctggtaat ctaaatttaa tgccagactt aggttctgct cctctagctt
    14341 atctgccaga aggtgctgat agcgacgaga tagaagagca atatctatat ttcatccctg
    14401 gaatacttag attttttcaa tataatatta aatttcaagc taatcaaaca aaagttgtag
    14461 atttaaataa attagcgagt tcaaataaaa atttattaac taataaaact atatatttct
    14521 gtcaagaaaa tttatctaat gaagtaaata atctagctaa tatctctaaa atatatactg
    14581 gtaaagtaaa aataactaat aaggatgaaa tttataaatt attactagat ggtgatccaa
    14641 atgcagctgt agcattcaca gtaaaaacta atggaaaaac aggattatat tgtataaaat
    14701 ttataataga tgctgatagt ggtactctca tatattatga tatgcatttg gttaaaaaca
    14761 aagacaatga cggttttact gcaaatgatt ttaaaaaatt gtaataattt tattgaatta
    14821 gacgcttagt tgataagaaa aaaatatttc gaaccattaa ttttcagatg atctcaatga
    14881 tgcaatatga tcgaggcaaa agaaaagccg atatgtggaa ggtctagaat atttagaaat
    14941 ttagcatcaa agatataaat caaacctttt gaactaagag caaagagccg tataataatc
    15001 aacaccttta tagtcatact tattactaaa ttcctatata ctcaaactaa tagatgccaa
    15061 ggtgatatga ttatcttagt tcagattttg taatcttact gttattttat tagtcaaacg
    15121 tctaattcat taattttttt ttttaaataa cataatgcat acttaattaa tgaattaatt
    15181 tataattaca atttcttata tttttcatat aaagtgaata tgcaaaaaat gctatatgtt
    15241 ttatttgtaa ataaatattt cagattataa agttttcaaa taatgttcat tttatttatt
    15301 cttaattttc tatctttgta attacttaac ccataaaata atgacaaaga attccgtttc
    15361 ttctttagct aagcaaactg tgatttatgg agctagtagt atacttggca gattattaaa
    15421 ttacctttta gtaccattat attcgagatt atttttacct gtagaggctg ctgtttatat
    15481 cgaattctat gcatatgctg ccatatttat agtgatatta acctatggga tggagacggc
    15541 attttttaga tttataaatg atcaaaatat aaaggataaa gatagagtat atagtacatc
    15601 ttttagttct atattattaa cctccagctt atttgttatt atattaagta ttttttctaa
    15661 tggtatagcc actagcttag gatatccagg taatgcagaa tatgtgattt tttttgcaat
    15721 agtagcaggt gctgatgcag tagctagtat tccatttgca tatcttagga gcaaaggcaa
    15781 agcaatgact tttgccagca ttcgcatcat taatattgtt gtaaatgttt ttttaaacat
    15841 attattttta gtaattatac ctaatgcata taataatcac cctgaattac aaagttttct
    15901 ttctatcttt acagatggta aaccatgggt aggctatatc tttgtagcta atgtagtaac
    15961 aagtttgcta aatttaatat tgctttttcc acattataag caagtaaaaa taaaaataga
    16021 taaaaaattg tggaataaaa tgctcaaata cgctataccg ctactaatat taggattatc
    16081 tggcatttta aataataata ttgatagaat tttattaaaa aatttattac caccagagat
    16141 agctttacat caaatgggag tatatgggat ggtatttaaa ttttcagtaa taatgagttt
    16201 ggccatacaa gcttttcgtt atgcggcaga tccatttttt ttcgagcaat caaaagacag
    16261 cgatgctaaa ggtgtatatg ctaaagtaat gaattatttt gtcattactt tagtaataat
    16321 ctatgctttt actatgctaa atatggatta tataaaatat tttgtgggta aagcatattt
    16381 cgaaggactg acagtagtta atattttatt acttagtcaa ttatttttag gtatttttta
    16441 taatttaagt atatggtaca aacttacaga taaaactatc atgggagcaa tagttagcat
    16501 aataggcact actattttaa taattttaaa tataatttta atacccaaaa tcggttacgt
    16561 aggtagtgca tgggctaatc tgatttgtaa tttttctatt gtgctaataa gttatttttt
    16621 atcacaaaag tattaccccg ttccctatga attaaagaaa ataatcgtat atattataac
    16681 ggggatcatt atagtatata tcggttcttt tgtccctact atcgacaatc attttttaaa
    16741 tattttagct cataatagtt taatcctttt atatattcct ataatttatt acttagaacc
    16801 aactttttgg ttatttataa aaaatacttt taaaagaaaa aaacattgat attatcatta
    16861 atacattaca actaattagt ataaattatt ttaataattc tgttctacgg gactttgagg
    16921 attaataggt atagacttcc attgcattgg ttctggtgga tcaggaaaaa gtggattttt
    16981 ttcataattt tttaagaaat cataaagttg atcaactgat aaacgtttag ctattatacg
    17041 tttatattgg tctaaaacat ataatacggg agtagaatat atatcataat attttctaaa
    17101 atctaccgat gctgtatata ttccattaac attaataaaa tcatacatat tatatgtttt
    17161 tattgctttt ttcatttcat taaaactact atctgtacat acagcaaata cttgcaaatt
    17221 gaaagtgtcc ttatatttat tgtaaaaatc aactaattta ggtgtttctt ttttgcaatg
    17281 tccgcaggta gggtcccaga aatacaataa tgtatattta gcattgatat tatgcaagga
    17341 tattaaattt ttagcagtat cgagtaaaat cagattagta gctatagtac cgatgagggt
    17401 gggacgtatt tcatttgctc tatctaatat tcgttgtaca gaagtttcag gcagccaaaa
    17461 agcttttcct gttttatagt aattatcagc tagataaaca aaaattttgt caaagcccat
    17521 cactttactt gtctcataat tatatgtcaa ccaccataac acatatttga aagtttcttt
    17581 atttttagat gctttagcta atactttatc tatttctttg ataatactat caggatgctg
    17641 cactattaca ttattaaaat aaaattctaa tttgttatga taaataggag tgcgtagcag
    17701 tgcttcatcg gccagatcta tattatccca ataatgttct ttgtaatagt aatattgata
    17761 agtactatca ggtttaccat cagttgtcaa tggtaatgta tcaggaattt cgggttcttt
    17821 agtagcttta aatattctag ctaaaagtgt attaggatat tgctccatat attttagctt
    17881 cattgtatta atgctatcta ttaaattttt tattttatca ttataatatt ttactgaatc
    17941 attcaaattt ttctgcttaa aatctttgac tttttgttgt aactggtata tttctttaga
    18001 aggcttagag attgttttat taaactgaaa aaaaagttca ttttctttag atccttttac
    18061 cttagcatta ccaagcaaat ctgtagtgtc agtttctatg gtaaaaattc tctcattatc
    18121 attcaatata aattctaaat atttttttga tggagtgatg agcaggtaca taccgctacc
    18181 tacagtatca ttggtatgaa atgtgcctcg cccattttta tccaatttga tagaatctct
    18241 cacatattgc catttaccgt agtaataacc caaataaaca tttgtatctg ctaattgtgg
    18301 tattataact tgaatcttgt aagcaggtgt tttttgtgct agtgatatat ttattaataa
    18361 aaagcttagt ataactatag caaaaaactt tttcatttta tttttttatt atgcaaatat
    18421 ataaaaattt atttttttta attgtaattt aatttttctt aattttgaat tattgatatt
    18481 gttagtcaaa atctttagaa tttgtttatt tgctaatttt gtcataaaaa aattgtcaat
    18541 ttttggcaat atttttattt tctattagta attttgcaat tgatgaataa aaatatattt
    18601 tttcttttat tattttgttt cgctttatct gatttagtgt tttctcaatc agaagataca
    18661 attactatca agcatgtaga ggcttatgag tggaatttca aaaattatct aattcaccct
    18721 gattctctta gatatgattt tgatacagtt ccattttttc atttttatcc ttataaagat
    18781 tataaataca atttaacaaa tgatttagta ggcgggatag tatatccagc tcggatacct
    18841 gatctgataa aacatcagaa aatatcgaat attagattgc cctacttgaa atttgttggt
    18901 ttttttgatc ccatagatat accacttatc aaatctgata cagctattat gcaagcacga
    18961 tttatgcaag gtatcaaacg tgaagagttt tttcaaactc atttcactat gccaatattg
    19021 gattttgcta attttaactt tttttatcgc actttttatg ctccttctac ttatgctaat
    19081 tcaatggtaa ggcaaaatca attttatatt aactttttcg gtaatactaa aaacaaaaaa
    19141 tataattatt atctcggact gattcagcaa aatagaacca caaaagaaaa tggcggtatc
    19201 aaaaatatga gttcattcgt agattctgct aagacagatc gtatacttct aaatatttat
    19261 ttaccagaag ctagtgctta tcacaaaatt aatgattatt actattcgca ttcatataaa
    19321 atcaataaat ttattgaatt atttcattat atatcatatt cacaatatag ctatagatat
    19381 actgataata aaccctctga agatttttac tatctttatg atggattgca aaatgatagc
    19441 atattcgatt cattaaacgt atggttacta gataatatag taggggtgaa agcaaatact
    19501 aaaaattggg actttaatat taactataat tattcttttg gcaaattcaa aatgaattta
    19561 ttaaaaagta aacctatctt tacttttatc aatgctgata ttaattttaa aaaatttgta
    19621 aatatatcaa tgcagtacta ttggggcgat tatcgtgatc aaaaagttta catatctggt
    19681 cattttcctt ctttttctta tggaataggt tataataaat tttcacaacc atatatttat
    19741 tcacactttt taggtaaata ttatcgttat gataagctat tacccgaaac agaaataata
    19801 tgggctaatt taaaaaataa aaataaattt ggtaatgttg cattatgtgg tggatatgta
    19861 aaaaagcaat tattttttga taaatatgga tttatcagat catcagatcc atatctattt
    19921 gctacattag aatttgattt aaggtttgat ttcggcaagc attttgtttt acagacctat
    19981 catttattcc aatatccata taacaaaaag acaatttcag taccagtatg gctcacacgt
    20041 aatgtatttt attaccatac tccattattc aaaggtaatg cacatctaaa gaccggtata
    20101 gaatttttgt atttgactaa atattatgct ccagcatgga atccaaatat tcagtatttt
    20161 gtagttagtg aaaataaatt aggtaatttc atttatcctt cattaaatat acaattaaaa
    20221 gtgaaaaaag caattatttt tgtacaactt gagaatttca cagatggtgt ttttaaaact
    20281 aggcaataca tgactatagc tggccatcct atgagagata tgacatttag atggggactg
    20341 caatggaatt tttataatta aaatattatg ttttttgatt ttatatataa tacaaaaata
    20401 ggggatcatt tgatgatccc ctattttctt ttttttaatc ttattctgga tgaatagcct
    20461 ctacagggca aacttctgca caagatccac aatctgtaca gagttctgga tcgattttgt
    20521 aaatatcacc aggagagata gcctctgctg ggcattcatc tatgcatgaa ccacatgctg
    20581 tacaatcttc tgttattacg tatgccataa ttattatgtt tttgttgtgc aaagataatt
    20641 aattttttta tttcaaaaaa tatataattt acattattga agtatgcata ctttagctaa
    20701 actcaatatt aatcgtaggt tatagtttat tatcttttta atataattaa aatgcttgat
    20761 ttatatcaat agatttgttg ttttatttac catatacaaa gccactttta ataatattat
    20821 gaataaaaat gtaaaggggt tagaggaatt tatcctttgt tttactttac gtagtttata
    20881 cttacatcaa ataattttta tttaatttta caaaaaaata attatggata attttaat
//
LOCUS       DAOPRN010000035        19699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_2355_length_19699_cov_158.916870, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000035 DAOPRN010000000
VERSION     DAOPRN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19699
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_19699_cov_158.916870"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PKI65_05250"
     CDS             complement(<1..288)
                     /locus_tag="PKI65_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77880.1"
                     /translation="MKNNKSSLFEIINDIDNKKLTIFLFIIASILYLPTITFDYTLDD
                     GLLIINNKFTQQGIKGIKDIFTHDAFEGALGEGAKYVAGGRYRPFTQFIFAI"
     gene            373..636
                     /locus_tag="PKI65_05255"
     CDS             373..636
                     /locus_tag="PKI65_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77881.1"
                     /translation="MEKKIGVILIVVQNRSAVSKLQEILSQYSYIIRSRQGLNLENEG
                     YSVITLVVQGDIDTLNALTGKLGRISGLKAKIITLNISNKEEI"
     gene            633..2066
                     /gene="hydG"
                     /locus_tag="PKI65_05260"
     CDS             633..2066
                     /gene="hydG"
                     /locus_tag="PKI65_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOB77882.1"
                     /translation="MKFNPEKCSLPDRPMEVFIDPDEIWEHLRTAKADKHAVRDIIQK
                     SLNKERLNLRETAILLNADDPDLIAEIKEGARELKKRIYGNRIVLFAPLYIGNRCINN
                     CLYCGFRVSNKESKRHTLSDEEIIKQVEALEDNGQKRLILVYGEHPDYSAEYIAHTVR
                     LVYSIRKGNGEIRRVNINAAPLEIDDFRTVKKAGIGTYQIFQETYNPEAYSWYHKGGK
                     KMDYSWRLTSLDRAQEAGIDDVGIGALLGLYDWRFEVMGLVRHTNHLEAVYNVGPHTI
                     SFPRIKRASGLTITDKYFVTDEDFIRLVAILRLAVPYTGMILTARESPRVRDEVIKYG
                     ISQIDGGTKLEIGAYAKKSLNEKQDLNAEQFEIEDSRSLEEIIDELTDMGFIPSFCTS
                     CYRKGRTGEHFMEFSVPGFIKRFCTPNALLTYAEYLCDYASPQLQEKGWKKIDEELKA
                     LEQDGYNTQNLISRLERVKLGERDLHL"
     gene            complement(2205..3443)
                     /locus_tag="PKI65_05265"
     CDS             complement(2205..3443)
                     /locus_tag="PKI65_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOB77883.1"
                     /translation="MIQSDTYKTFVILTQYFPPEIGAPQYRLYELAKSLKNNKFRVIV
                     LTALPNYPKNEIYEGYRCEKYRFDIMDDIEVHRFTLYIPKKRTPFKRLFQYISFAYNV
                     IKYSKRVLTEEIDYLFWETPPIFLGWAAYVLKKRTGAKLITNVSDLWPESIEKLGIIK
                     NKIILKPLYWFESFLYKKSVLVTGQTKGIISNISNRFPKVKTYWLPNGISADLFEYSY
                     TKPTIWRKNHNFDENDFLIVYAGIIGYAQGLEIVIEAAKKLNNYPIHFLIFGEGPELY
                     KLESRINEVTNVHLMGTIPRKEIPTIIRAEDVALIPLRNIDLLQGAIPSKIFEHLAFA
                     KPLILGIKGEAYELFIKQANAGVSYEPDDPISLANAAIYLYENKDLCIKLGKNGKKFV
                     LDNFDRAEISKKFVNLINRL"
     gene            complement(3565..5664)
                     /locus_tag="PKI65_05270"
     CDS             complement(3565..5664)
                     /locus_tag="PKI65_05270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOB77884.1"
                     /translation="MKKYFFISILFLLWTLLFISCKKTSIEAEKIIGPDSLKFESNIL
                     TPEKLWYFGRISDMQLSTDKNKLLFGVMWYDWQENKGNRELYIIDLNTKQRQRITTTP
                     KSEYNARWCPDGQKIGYLYPDKNGDLQLFEMNIDGSKIEQISYIKGGVNGFEYAPDQK
                     HIAFFKDVKIIPDPEDIYSDLPKANARIYEDLLPRHWDQWVKSFSHLFIAPYSDKIDT
                     VGKDVMYTQAYECPLYPFGGNEQINWHPDGKQLAFTAKAMIGRDYALSTNSDIYIYDL
                     DKDDLWNMTKGMMGFDQNPVYSPDGKKIAWESMEREGYESDRIRPFIYDFTKDTMWEA
                     VENYETHATNLKWSPDGEFIHFISPWHGTQQIYLVDIYKKKPLAITSGTHNYHDFLIS
                     KQGIIGIKQSMSKPDEIYIIDPLSGAESELSFINKYWLDQLQMGEVRPYWINTIDKKK
                     MLVWVIYPPHFDSTKKYPTILYCEGGPQSMVGQFWSYRWNFQLMAANEYIIVAPNRRG
                     LPGFGIEWLEQISGDYCGLNIQDYLTAIDTFATVCYVNKDALGAAGASYGGYSVYYLA
                     GVHNGRFKAFISHSGMFNLESQYLETDEYWFVNWDLGGPYWDKNNKIVQRTYANSPHK
                     LVDKWDTPILVMHGENDFRISYTQGLQAFQAARLRNIPAKLVIFPEENHWILKPQNAI
                     LWQREFFGWFDKYLKNQ"
     gene            complement(5839..7977)
                     /locus_tag="PKI65_05275"
     CDS             complement(5839..7977)
                     /locus_tag="PKI65_05275"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOB77885.1"
                     /translation="MAVIEKIRKRSLLLLIAIGGAMILFILSDLLWKGGGRRRIDPIA
                     KVLNEAISYEEFDYKILEQEEFFKMQYGDNYHFTPIERHQINNYAIDQLINNKIIDKE
                     LRKANIFISKAEISSLFTGRLIHPFIRQIFTDPNTGNFDPRVVQGYLDNLETLPEAEQ
                     LRWFAIENTILEDHKFQKYFNIISKAYYIPTPILNKQVNDYNTERTVQYLGLKYSTIS
                     DNEIQLSETDLKKYYEEHLYMFQTDPFVDIDYVIFDVLPSASDMKKVELYIKEAYDSL
                     MSASEHRIPIIVNKFSKEPFDSIYIKLSDLPPQADSLANAKKGTIIGPYLYNNTWYIN
                     MFLDKKIIPDSVKARHLLIAYQGAMRSSPNITRTKEEADKLADSLFVILKSTGDTALF
                     NEFVKKYSDDQGSIPQNGDLGWFTEGIMIKPFNDACFSTPEGQFTKIETDFGIHIIYV
                     EKKTKPIEKYLVANIVFPLEPSNETMDSIYNIANNFAIMAKTANDFEDLIISKGYNKR
                     TAERIKKDDFTIPGIENGREIIRWAYNKETKVGTVSAVFDLNYENKEVVALLKYRQDE
                     KKPYLAYEKVKEQLRPFVLKEKKAEILIKKINELKANDINTLSQKLNTELDSAVITFY
                     TYSLPFYGPEPKLVGAVSSLPLQVISHPIKGETAVFVARVFEEHQRSNVEINTLRLQE
                     NQMHTSKINYELLNSLKKVYKVIDNRTMYF"
     gene            8073..9305
                     /locus_tag="PKI65_05280"
     CDS             8073..9305
                     /locus_tag="PKI65_05280"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB77886.1"
                     /translation="MRNIFTLFLIICISLSTFIISKIHAQESAYDFFLIGKNMYDAQN
                     YKQAIHNFDKAISLNTFYYDAYLEKAKCYLAINNKSEALNEINKVINLKRNYAPALYM
                     RAEYWMNEKQYKQAITDLKEVVLQNKYYYPARELLANAYYNINDFQNALNEINFLISE
                     QPTEYKYLLKRAHINKDMKQWNEAIKDCNTLISKNKFANEAYALRGTILLEQNKNNEA
                     FDDFSRAIANNYFTKELLLIYLNMSMKNDKLINSQQALELLIKNYEPKNSYYHLLLGK
                     ILIKEQKYDIAISVLKNAISLRPNYDTAYVYLGMAKRLISKDPINDLRKAIYLNPKNS
                     LAYEELGIYYIDKKLYSQALDNLSTAIKLNPTGRAYYYRAIVWDLNRNKENACLDLKK
                     AKELNYKEAQIAFNKICM"
     gene            complement(9835..11781)
                     /gene="gyrB"
                     /locus_tag="PKI65_05285"
     CDS             complement(9835..11781)
                     /gene="gyrB"
                     /locus_tag="PKI65_05285"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOB77887.1"
                     /translation="MNEVNAINDYNANNIQVLEGLEAVRKRPAMYIGDTSERGLHHLV
                     YEVLDNSIDEALAGYCDNIKVIIHNDNAITVMDNGRGIPVDMHPIENRPALEVVMTML
                     HAGGKFDKDTYKVSGGLHGVGVSCVNALSKKMIATIKRDGKIWQQTYECGKPITELQC
                     IGETMETGTTIYFEPDDSIFQNIDFKYDILSKRLRELAFLNRGVTIELMDERFNPDEP
                     RISTFYYENGLIDFIHYLDESRQVLMPEPIYIEGEKDNIKVEIAFEYNDSYIENVHTF
                     VNNINTIEGGTHLAGFRRALTRTMKAYGDEQGYFDKVKFEISGDDFREGLTAILSVKV
                     PEPQFEGQTKTKLGNSEVAGAVDSIVSEHLSYYLQEHPKETREIFNKVVLAASARHAA
                     RKAREMVLRKTVFSSAQLPGKLADCSETDPAKCEIFLVEGDSAGGTAKQGRNRLFQAI
                     LPLRGKILNVEKAADHRVFESEEIRNIYTALGVTIGTPDDPKALNLEKLRYHKVIIMT
                     DADVDGSHITTLILTFFFRYMRELIEKGYVYIAAPPLYLMRQGNKKIYCWSDDERDKI
                     LAEWKQTGDKRNIHIQRYKGLGEMNAEQLWETTMNPETRILNRVTIENAAEADRVFSM
                     LMGEDVPPRRQFIEANARLAKIDA"
     gene            12270..12695
                     /gene="tsaE"
                     /locus_tag="PKI65_05290"
     CDS             12270..12695
                     /gene="tsaE"
                     /locus_tag="PKI65_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOB77888.1"
                     /translation="MQLIDKYYDVSLEDLPILAKNLEELFTKRNIICLQGEIGAGKTT
                     LAKQIVKNMLGSDHAISPTFSILNIYNNDNISIYHIDLYRLNDIKEFFDLGLYEYLSD
                     NYVLIEWPELILNYLKDFDFYLVKINIINETKRLIEIWG"
     gene            12692..13903
                     /locus_tag="PKI65_05295"
     CDS             12692..13903
                     /locus_tag="PKI65_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOB77889.1"
                     /translation="MKKHYFYGPTGEFLPLEKRLEVKNNQKKFVIGIPKEIAFQEHRV
                     SLVPDSVKMLVHNGHEVLVEDGAGEYARFSNHDYAEVGGEIVNSHVDILARSDIIVKV
                     APLLMQEIQYMKKNQIVISAVNYYSQSKEYFESLIERNAIAIGFEFIKDNSGRYPVLM
                     AMSEIVGYGVIQLAARYLSDNKISKGYLFGGLPGLNPTEVVIIGAGRVGLFSAKAAKA
                     FGALIKVFDNKIYKLRSIVSELGPDIYTSVLHPEVIIKALKTANVVICALYMNDGELS
                     FLLTEDMVKEMKEGSIIFDVSIDQGGCCETSEITYIDDPIFVKHGVIHYCVPNIASNF
                     PRTAAYALSNFFTPLIIDIADNGGIKEYLRINPYFSQGTYILDEKICHPLIAQHFNFK
                     YTPIQLLLGGF"
     gene            complement(13973..15316)
                     /locus_tag="PKI65_05300"
     CDS             complement(13973..15316)
                     /locus_tag="PKI65_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77890.1"
                     /translation="MRLFPDIIAEQVSTNKWLIRKLTEENLVMLTDSIAKIIINPFLD
                     LSIGIDKTNNTKINFVNTRGVYMMAQIGKKLYLSSEFYENQAYLPRYYMSLIDSIGFL
                     PGNNWVKKYRNRGLDYGLALGRIQWQATNWFAIESGFNTLNVGHGYRSLILGYDARPY
                     PYIHFVFNYQKKIILGNITGIAYHNDNVVDITEISTRNIFSVNYFTFNPYDFLSISLL
                     DFTIFHPAKKQLRFSPYTFILIPQLRPFILQNKPSWSLPGYAINFYLPNNDIYFQNTI
                     NIDDAIDKNFKNKLAYQIGILSRIKFKDTSLFFNVRLEYNNVAEHAMDNEYYSTYPTH
                     LSQPLGMWQGENLKEIILQLNILKNRHLFQLFSSYQNIKISPFNLNNYLGQINNIYTS
                     ITYTYLINPVAGLGVFAELQFRIPFVNDFYINNALGVKCGIKYLFRDKSINRWHL"
     gene            complement(15481..17118)
                     /gene="ade"
                     /locus_tag="PKI65_05305"
     CDS             complement(15481..17118)
                     /gene="ade"
                     /locus_tag="PKI65_05305"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOB77891.1"
                     /translation="MRKYIISGNIVDVINSEIYKGTIKIKNGRIKNIERNDNILCENY
                     ILPGLIDAHVHIESSLVAPSFFAAEAVKHGTIGVVADPHEIANVMGVKGIDFMIENGK
                     TVPFHFFFTAPSCVPATAFESSGSVIDAEIINSLLAKPDFYALAEMMNFPGVINNDIE
                     VVKKIEAAINHHKPIDGHAPLLSNDELKKYIDAGISTDHECTNIEEAREKIKLGMKIL
                     IREGSAAKDFDNLWPLLLENPDKIMFCTDDLHPDHFIKGHINKLVRKAIANGVDPITA
                     IRAASLNTAQHYRLPVGFLQKDDPADLIVIDDFNNFNVLQTFIKGNLVYDHGKVNFTS
                     RINQLVNNFTINKISIDDLKLKATSNNIQIIKAFDGSLLTEKLVTTVTIDEDGFAISD
                     QNRDVLKIVVLNRYESGAKPAIGFINGFELKNCAIGSSVAHDSHNIVAIGTQDRFIVE
                     AIELIQKHKGGLVFVSDKNTHILPLPIAGLMSDMEVVKTADSYETLNKYTTEYGSNMH
                     SPFMTLAFMSLLVIPHLKLSDKGLFDVDSFSFTDLFV"
     gene            17288..18760
                     /gene="proS"
                     /locus_tag="PKI65_05310"
     CDS             17288..18760
                     /gene="proS"
                     /locus_tag="PKI65_05310"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOB77892.1"
                     /translation="MNKFITPRNVDYSQWYLDIIEHAQLAENSVVRGCMVIKPYGFAL
                     WENIQATLDKMFKDTGHINAYFPLFIPKSFFSKEAEHVEGFASECAIVTHYRLKKDPN
                     SKGLIIDDTAKLEEELVVRPTSETIIWSTYRNWIQSYRDLPLLINQWANVVRWEMRTR
                     LFLRTTEFLWQEGHTAHATKEEAMEEARLMLNIYASFAEQYMAIPVIKGVKTANERFA
                     GAEDTYCIEAMMQDGKALQAGTSHFLGQNFAKAFDVKFLDRNNELQYVWATSWGVSTR
                     LIGAIIMTHSDDKGLVLPPKLAPTQVILIPIFRTEEEKSKVLEKLNNIKNQLIENNIK
                     VKLDDRDEFSPGWKFTEWEMHGVPLRLAIGPRDIDNNTVEFARRDTGEKHILPQDNII
                     ENVKNYLENIQKSLFEKALDFRDAHTYIIDTWDEFIEKIEQGNFIWAHWDGTTETELK
                     IKELTKATIRLIPLSPNLENGSCILTGKPSKYRVLFARAY"
     gene            18904..>19699
                     /locus_tag="PKI65_05315"
     CDS             18904..>19699
                     /locus_tag="PKI65_05315"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77893.1"
                     /translation="MDNFNLLDYQALPKEQKRRFLDNLYQFLLENNYTAVDYQKLKIQ
                     STAPICPRCESENVIKAGVRDGKQVYKCKDCGRQYRETARTFVYRMRKADKMLDYLKC
                     MLEGKSLRACASEVGISLRTSFNWRHKILAAIKSLQGGISFSGIVEADELLMKYSEKG
                     RKYRSRKEYEKAKKKKHPKVAVLVMTDREGNLLFKHVGRKKVTNSQIREELSHRVTDS
                     NLICVKQKKEFKKAVKGTKSKKVVVNKTQIRRGVYSVQVVDKHINDF"
BASE COUNT         6916 a         2955 c         2938 g         6890 t
ORIGIN      
        1 aatagcaaag ataaattgag taaatggacg atagcgaccg ccagctacat atttagcacc
       61 ttcacctaag gctccttcga aagcatcatg agtaaaaata tcttttatcc cttttattcc
      121 ttgttgtgta aatttattat taatgattaa tagtccatca tctagagtat aatcaaaagt
      181 aatagttggt aaatataaaa tactagcaat tataaataaa aaaatagtaa gctttttatt
      241 atcaatatca ttgattattt caaataaaga acttttatta tttttcatga aattttattg
      301 cgaatttaat atttttttat cataaaaata ttttgtaatt ttgcataaac taatccaaaa
      361 gaatttgctc tgatggagaa gaaaataggt gttattctca tagtggtaca aaatcgttct
      421 gcagtttcta aactccagga gattttgagt caatattctt atattattag gtctcgtcaa
      481 ggtttaaatt tagaaaatga aggttatagt gtgataactt tggtagtaca aggtgatatt
      541 gacacattaa atgcacttac gggcaaactc ggacggatta gtggcttaaa agccaaaatt
      601 attactttaa acatatcaaa taaggaggaa atatgaaatt caatccagaa aaatgttcgt
      661 tgcccgatag accaatggag gtatttatag atccagatga aatatgggaa catttaagaa
      721 ctgcaaaagc tgataagcat gcagtaagag atatcatcca aaaatctctt aataaagaac
      781 gcttaaattt acgagaaacc gcaatattgc tcaatgccga tgatccagat cttatagctg
      841 aaattaaaga aggagcacgt gaattaaaaa aaagaattta tggtaatcgt attgtgcttt
      901 tcgcaccttt gtacataggt aatagatgta ttaataattg cttgtattgt ggctttcgcg
      961 ttagtaataa agaatctaaa cgtcatacgc tgagcgacga agaaataatt aaacaggtag
     1021 aagctctcga agataatgga caaaaacgtt taatccttgt ttatggcgaa cacccagatt
     1081 attctgctga atacatagct catactgtta gattggttta tagtattaga aaaggaaatg
     1141 gcgaaatacg tagagtaaat ataaatgctg caccattaga aatagatgat ttccgcacag
     1201 ttaagaaagc cggcataggc acatatcaaa tattccaaga aacttataat ccagaagctt
     1261 atagctggta tcacaaaggt ggaaaaaaga tggattactc atggagactg acttctctgg
     1321 atagagctca agaagcgggg atagatgatg tgggtatagg tgcactatta ggtttatatg
     1381 attggagatt cgaagtaatg gggttggtca gacatactaa tcatctcgaa gctgtatata
     1441 acgtgggtcc acatactatt tcatttccac gaatcaaaag agcatctggc ttgaccatta
     1501 cagataaata ttttgtgact gatgaggatt ttatccgttt agttgctatt ttgcgcttgg
     1561 cagttccata tacaggaatg atattaactg ctcgcgaaag tcctagagtt cgcgacgaag
     1621 tcatcaaata tggtatcagc caaatagatg gtggtactaa gttagaaata ggtgcttatg
     1681 ccaaaaaaag tcttaacgaa aagcaagatt tgaatgctga acaattcgaa attgaagatt
     1741 ctagatcttt agaggaaata atagatgaat tgactgatat gggattcata ccgtcgtttt
     1801 gcacatcttg ttatcgtaaa ggtagaactg gtgagcattt tatggagttt tctgtacctg
     1861 gtttcataaa acgcttttgt actcctaatg ctttattgac ctatgctgaa tatctttgtg
     1921 attatgcatc tccgcaatta caagaaaaag gatggaaaaa aattgatgaa gaactcaaag
     1981 cattagaaca agatggatat aatactcaaa acttgattag tagattagaa agagttaaat
     2041 tgggcgaacg agatttgcat ttgtgaaatc aactatttaa tgtttagtaa aattaaaata
     2101 ttaattcagt atgtgccaat atgcataatt aacaacctac aaaggacaac aaacaatttg
     2161 ctaaatttct aaactactaa acctttatct tttgtcttta ttaattataa tctgttaatt
     2221 agatttacaa attttttact tatttcagct ctatcaaaat tatctaaaac aaatttttta
     2281 ccattttttc caagttttat gcaaagatct ttattttcat ataaataaat agcagcatta
     2341 gccaaagata taggatcgtc tggttcgtaa ctaacccctg cattagcttg ctttataaaa
     2401 agttcataag cttccccttt tattcctaaa atcaagggtt tagcaaaagc aagatgttca
     2461 aaaatttttg acggaatggc accttgcaat aaatcaatat ttcttaatgg tatcaaagct
     2521 acatcctcag ctctaattat agttggtatt tcttttcttg gtatagttcc catcaaatgc
     2581 acattagtaa cttcattaat ccgagattct aatttatata attctggacc ttctccaaaa
     2641 atcaaaaaat gaataggata attatttaat tttttagcag cttctattac tatttctaat
     2701 ccttgtgcat atccaattat tccagcataa actattaaaa aatcattttc atcaaaatta
     2761 tgattttttc tccatatagt aggttttgta tatgaatatt caaataaatc tgcacttata
     2821 ccattaggca gccaataagt ttttactttt ggaaagcgat tagagatatt tgaaattatt
     2881 ccttttgttt gcccagtgac taaaacggat tttttgtata aaaaagattc aaaccaatat
     2941 aaaggtttta gaattatttt atttttaata attcctaatt tttctatgct ttccggccat
     3001 agatcactta catttgttat tagcttggca ccagttcgtt tttttaatac ataagcagcc
     3061 caacctaaaa atattggagg ggtttcccaa aataaataat ctatctcttc tgttaaaact
     3121 cttttcgaat atttgatcac attatatgca aatgaaatat attgaaataa tcttttaaaa
     3181 ggcgttcttt ttttaggtat ataaagtgta aatctatgca cttctatatc atccattatg
     3241 tcaaatctat atttttcgca cctgtatcct tcataaattt catttttagg ataatttggt
     3301 aatgcagtca atacaattac tctgaattta ttatttttta atgatttagc tagttcatat
     3361 aatctatatt gaggtgctcc tatttctggt ggaaaatatt gtgttaatat tacaaaagtt
     3421 ttgtatgtat cagattgtat cattttaata tttattattt gtcaaaatta aagataattt
     3481 caatttgata agtacgaaag aaattagtat atatttattg ttgataattt attttgtcga
     3541 ataaatattt aaaaaaataa aagtttattg atttttcaga tatttatcaa accatccaaa
     3601 aaattctctc tgccataaga tagcattttg aggttttaat atccaatgat tttcttcagg
     3661 gaaaataaca agcttagcag gaatatttct taaacgagca gcttggaagg cttgtaatcc
     3721 ttgagtatag ctaatgcgaa aatcattttc tccatgcatc actagtatag gagtatccca
     3781 tttatctaca agcttatgag gagaattagc atatgtacgt tgtactattt tattattttt
     3841 atcccaataa ggaccaccga gatcccagtt tacaaaccaa tattcatcag tttctaaata
     3901 ttggctttct agattaaaca tcccagaatg tgatatgaat gccttaaatc tgccattatg
     3961 cacacctgct agataatata cagaatatcc tccatagcta gcaccagcag cacctaatgc
     4021 atctttattt acataacaaa cggttgcaaa agtatctatt gctgttaaat aatcttgaat
     4081 atttaaccca cagtaatcgc cactaatctg ttctaaccat tctataccga atcctggcaa
     4141 gcctctacga ttgggagcta ctatgatata ttcattagct gccattaatt gaaaattcca
     4201 tctatagctc cagaattgac ctaccatgct ctgaggtcca ccttcgcaat ataatatcgt
     4261 tgggtatttt ttagtgctat cgaaatgagg cggataaatt acccatacca gcattttttt
     4321 cttatctatt gtatttatcc aataggggcg cacttcaccc atctgcaatt gatctaacca
     4381 atacttatta ataaatgata gttcgctttc tgcaccactt aatggatcta ttatatagat
     4441 ttcatctggt ttagacatag attgtttgat gcctattata ccttgtttag atattaaaaa
     4501 atcgtgataa ttatgtgtgc cactggttat agctaaaggt ttctttttat agatatctac
     4561 tagatagatt tgttgagtac catgccatgg actaatgaaa tgaataaatt ctccatctgg
     4621 ggaccatttt agattagtag catgagtctc ataattttct actgcctccc acatagtatc
     4681 ttttgtgaaa tcatagataa atggacgaat gcgatcagat tcgtagcctt cacgttccat
     4741 gctctcccaa gctatttttt taccatcagg actataaact ggattttgat caaatcccat
     4801 catacctttt gtcatattcc atagatcatc tttgtctaaa tcataaatat aaatatcgga
     4861 attagtagac aacgcatagt ctctacctat catggcttta gcagtgaaag ctaactgctt
     4921 accatcagga tgccaattaa tttgttcatt accaccaaaa ggatatagtg gacattcata
     4981 agcttgagta tacatcacat ctttgcctac agtatctatt ttatcactat agggagctat
     5041 gaatagatgc gaaaaagact tgacccactg atcccaatgt ctgggcaata aatcttcgta
     5101 tattctagca tttgccttag gcagatcgga atagatatct tcgggatctg gtattatttt
     5161 tacatctttg aaaaaagcaa tatgtttttg gtctggagca tattcgaagc cgtttactcc
     5221 acctttgata tatgatattt gctctatctt actaccatct atgttcattt caaataattg
     5281 caaatcacca tttttatcag gataaagata tccaattttc tgcccatcag ggcaccatct
     5341 agcattatat tcactcttag gagtagtagt gattctttgt ctttgttttg tatttaggtc
     5401 tattatatag agctctctat tgcctttatt ttcttgccaa tcataccaca taacgccaaa
     5461 aagtaattta tttttgtcag tagaaagctg catatcagag atacgaccaa aataccaaag
     5521 tttctcagga gttaagatat tagactcaaa ttttaaacta tcgggaccaa tgattttttc
     5581 ggcttcaata cttgtttttt tacaagatat gaataacaat gtccataata aaaataaaat
     5641 agaaataaaa aaatactttt tcataataaa ttctttttga gaaccaaaaa tataaaatta
     5701 aaatgtaaat aaaaaatttt gaattttaaa aactttaata ttcattaaag taattacctt
     5761 gtcacatcgg tagttatgaa tttatatttt tgatttactt tagttaaagc aaaaagattt
     5821 taaaaaatat caggttattt aaaaatacat tgttctgtta tcaataactt tgtaaacctt
     5881 ttttaaagaa tttaataatt catagttaat tttagacgta tgcatttggt tttcttgtaa
     5941 tctgagcgta tttatttcta cattgctgcg ttggtgctct tcaaaaactc gagcaacaaa
     6001 tactgccgtt tcacctttga taggatgact aatgacctgt aaaggtaaag aagaaacagc
     6061 acccacaagt ttaggttcag gaccataaaa aggtaagcta tatgtataaa aagttattac
     6121 agctgagtct aattcagtat ttaatttttg tgacaaggta tttatatcat tagctttgag
     6181 ttcattaatt tttttgataa gtatctctgc ttttttttct tttaaaacaa aggggcgtaa
     6241 ttgttctttt actttttcgt aagctaagta aggttttttt tcatcttgcc tatattttaa
     6301 taaagctaca acttctttat tttcgtaatt caaatcaaag acagcagaca ctgttcctac
     6361 ctttgtttct ttattgtaag cccaacgtat aatttcacga ccattttcga taccaggtat
     6421 agtaaaatca tcttttttaa tacgttctgc tgttcgttta ttataacctt tgctaattat
     6481 tagatcttca aaatcattag cagttttagc cattattgca aaattattag ctatattata
     6541 tatgctgtcc atggtttcat tgcttggctc taaaggaaaa actatattgg ctacaagata
     6601 tttttcgata ggtttggttt tcttttctac ataaataata tgaataccaa aatcggtttc
     6661 tatttttgta aattgtcctt caggtgtaga aaagcaagca tcattaaaag gttttatcat
     6721 tataccttct gtgaaccaac ctaaatctcc attttgtggt atactacctt ggtcgtcact
     6781 atattttttt acaaattcat taaataatgc agtatctcct gtagatttta gaatgacaaa
     6841 taaactatct gctaatttat cagcctcttc ctttgtacga gtaatattag gcgaacttct
     6901 catagcacct tgataagcga tgagtaaatg tcgagctttt acgctatcag gaattatttt
     6961 tttgtctaaa aacatattaa tataccaagt attattgtat aagtaaggac cgattattgt
     7021 acctttttta gcatttgcta atgaatcagc ttgaggtggt aaatcagata attttatata
     7081 aatagaatca aatggttctt tagagaattt atttacgatt attggaattc gatgttcgct
     7141 agctgacatt aatgaatcat aggcttcttt tatatataat tcgacttttt tcatatcaga
     7201 agcagaaggt aatacatcga aaatcacata atcaatatct acgaaaggat cagtttggaa
     7261 catatataaa tgctcttcat aatatttttt taagtcagtc tcagacaatt gtatttcatt
     7321 atcactgata gtagaatatt tcaatcctag gtattgaacc gttcgttcag tattgtaatc
     7381 atttacttgt ttattaagga taggagtagg gatgtaatag gcttttgaaa taatattaaa
     7441 atatttttga aatttatgat cttctaatat agtattttct atagcaaacc atcgtagctg
     7501 ctcagcttct ggcaaagttt ctaaattatc taaataacct tgcactactc taggatcaaa
     7561 gttcccagtg ttaggatctg taaaaatctg tcttataaaa ggatgtatta gtctgccagt
     7621 aaataaactt gatatctcag ctttacttat aaaaatattt gcttttctta attctttgtc
     7681 aattatttta ttattaatta attgatctat agcataatta ttaatttggt gcctttctat
     7741 tggagtaaaa tgataattat ctccatattg cattttaaaa aattcttctt gttctaatat
     7801 tttataatca aattcttcat aactaatagc ttcatttagt acctttgcaa ttggatcaat
     7861 tctgcgtcta ccaccacctt tccataataa atcacttaat ataaaaagta tcatagctcc
     7921 acctattgct attaacaata aaagtgaacg ctttctaatt ttttcgataa cagccataat
     7981 ttatattttt atgcaaaaat aatttaaaat ttttaaaaat aagaattatt atttatactt
     8041 aattttgaaa aacaattaaa atttagtttt gaatgaggaa tatttttact ttatttttga
     8101 ttatttgtat ttcattatcc acttttataa tatcaaaaat acatgctcaa gaatcagctt
     8161 atgatttttt tttaataggt aaaaatatgt atgatgctca aaattataag caagccatac
     8221 ataattttga caaagctatt tctttaaata ctttttatta tgatgcttat ttagaaaaag
     8281 ctaaatgtta tttggctatt aataataaat ctgaagcttt aaatgagatt aataaagtta
     8341 ttaatttaaa aagaaattat gctccagccc tatatatgcg tgctgaatac tggatgaatg
     8401 aaaaacaata taaacaagct atcacggacc taaaagaggt agttttacaa aataaatatt
     8461 attatcctgc tagagaatta cttgctaatg cttattataa tattaatgat tttcaaaatg
     8521 ctttaaatga aattaatttt ttaatatctg aacaaccaac tgaatacaaa tatttactaa
     8581 aaagagctca tataaataaa gatatgaaac aatggaatga agctataaaa gattgtaata
     8641 cattgattag taaaaataag tttgccaatg aagcatatgc tctgagaggt actattctat
     8701 tagaacaaaa taaaaataat gaagcttttg atgatttttc gagagctata gcaaataatt
     8761 attttactaa agaattactt ctgatttatc tgaatatgtc tatgaaaaat gataaattaa
     8821 ttaattcaca gcaagcatta gaattattaa taaaaaatta tgaacccaaa aactcatatt
     8881 atcatctgtt attaggtaaa attttaataa aagagcaaaa atacgatatt gctattagtg
     8941 ttttaaaaaa tgccatttct ctacggccaa attatgatac agcatatgtt tatctaggta
     9001 tggctaaaag attgataagt aaggacccta ttaatgattt acgtaaagct atttatttga
     9061 atccaaaaaa ttctttagca tatgaagagc ttggtattta ttatattgat aaaaaattat
     9121 attctcaagc tctagataat ttgagtactg ctattaaact taatccaact ggtagggctt
     9181 attattatag agcaatcgtg tgggatttga atagaaataa agaaaatgcc tgtttagatt
     9241 tgaaaaaagc taaagaatta aattataaag aggctcaaat tgcatttaat aaaatctgta
     9301 tgtagttaat taaactctca ggcatctata tttatttttt ttcaactatg taagtgtaaa
     9361 tagttataga tttatttaca aatcattatt ttgagataaa tggaggttct ataacgttta
     9421 ttgtgggtaa aaacatttta gattgttaca taaaaataac tatctttaat tatttaggtt
     9481 gtttatttta ccacagagaa caccaagtta ttacacaaag tccacaaagt gccatttatg
     9541 gtccttttaa tttcatcctt ctctgtgttc tttgcgcatt ctttgagaac tttgtggtta
     9601 atatttaact atactataat taataaacta atattaacaa tttttttata aaactactct
     9661 ttttttaatc tttacccata caaaacgtta tagaacctaa atggaggttt gaaaacaaat
     9721 taaatacaaa taaaaaaaat tataatttat taaacacaca cgaagtcatt tagataagta
     9781 ttgggctaat gatataacga tcatcattaa tgttttaaaa aacatcataa taaattaagc
     9841 atcaattttt gccaatctag cgttagcttc tataaattgc ctacgaggcg gtacatcctc
     9901 tcccatcagc atagaaaaaa ctctatctgc ctctgcagca ttttctatag taactctgtt
     9961 aagaattcta gtttcaggat tcatagtagt ctcccagagt tgttcggcat tcatttcgcc
    10021 tagaccttta tatctttgaa tatgaatatt tcttttgtcg ccagtttgtt tccattcagc
    10081 taatattttg tctctttcat catcagacca acaataaatc tttttattac cttgacgcat
    10141 tagatataga ggtggtgctg ctatatatac ataacctttt tcaattaatt ctctcatata
    10201 tctaaaaaag aaagtaagaa taagagtagt tatgtggctt ccatctacat cagcatcagt
    10261 catgattatt actttgtggt aacgtaattt ttctaagttt agagccttgg gatcatctgg
    10321 tgtaccgata gttacaccta aagctgtata aatattacga atttcttcac tttcaaaaac
    10381 tctatgatca gcagcttttt ctacatttaa gattttacct cttaaaggaa gtatagcttg
    10441 aaaaagtcta ttacgacctt gcttagcagt gccaccagcg gagtcacctt caactaaaaa
    10501 gatttcacat ttagcaggat cagtttcaga gcagtctgct aacttacctg gcaattgtgc
    10561 cgaactaaaa acagtttttc ttagcaccat ttcgcgtgct ttacgagctg catgacgtgc
    10621 tgatgctgct aaaactactt tattaaaaat ctcacgagtt tcttttggat gctcctgtaa
    10681 ataataagat aaatgttcgc tgacgataga atctactgca ccagctactt ctgaattacc
    10741 taattttgtt tttgtttgac cttcgaattg tggctctggc acctttacac tcaatatagc
    10801 tgtaagaccc tccctaaaat catcacctga gatttcaaat ttcactttat caaaatatcc
    10861 ttgttcatcg ccataagcct tcatagtacg agtgagcgcc cttctgaaac cagccaaatg
    10921 agttccacct tctatagtat taatattatt aacaaaagta tgaacgtttt cgatataaga
    10981 atcattatat tcaaaagcaa tttctacttt gatattatct ttttcacctt caatatatat
    11041 gggttctggc attaatacct gcctcgactc gtctaaataa tgaataaaat ctattaatcc
    11101 attttcataa taaaaagtac taatgcgggg ttcatcagga ttaaatctct catccattaa
    11161 ctcaatagtt acaccacggt ttaaaaaagc aagttcacga agtcgtttag atagtatatc
    11221 atacttgaaa tcaatatttt gaaaaatcga atcatcaggt tcgaagtata ttgtagtacc
    11281 agtttccata gtttcgccta tacattgtaa ttcagtaatt ggtttaccac attcatatgt
    11341 ttgttgccat attttgccat cacgtttaat agtagctatc atttttttag ataaggcatt
    11401 tacacatgat acacctactc catgtaaacc accagaaacc ttgtatgtat ctttatcaaa
    11461 tttgccgcca gcatgaagca ttgtcataac tacttctagg gctggtctat tttctatagg
    11521 atgcatgtct acaggaatgc cacgcccgtt atccataacg gtaatagcgt tatcattatg
    11581 aataattact tttatattat cacaataacc agccaaagcc tcatcgatag aattgtctaa
    11641 tacttcatag actagatgat gaagtcctcg ctcactcgta tcacctatat acattgctgg
    11701 tcgcttgcgt acagcttcta gtccttctaa tacctgtatg ttattagcat tataatcgtt
    11761 tattgcatta acttcattca taaatttaat ttttagcttt atgcaaagtt aataattttt
    11821 gaaaatataa acaaatattt agccataatt ataatgatta atgtgttttt gacttaattt
    11881 ataatttatg gttattatta ctataaataa tcaatgtttt agtcattgac ataggttcta
    11941 attttgagat gcatgaaatt atctgtattt tatcaataat tttttatttt aaaaaaaaat
    12001 tattaatttt gcaaagaagt attcttcatg atttttttaa attttttcat aattaaaaaa
    12061 ggaattcatt taaatgaatt ccttttttat tcaatttcta attttttaat taaatttgct
    12121 tttaatattt ttttttaact tttcttaaaa gttattattt tttatcaatg taaaaaaaat
    12181 gatgtatcat tttttcaata tcaagatttt caaaccttac atttggtttt ttaaatatat
    12241 taagtatttt aatatgttaa ttcagagtta tgcagttaat agataaatat tacgatgtta
    12301 gcttagaaga tttgcccatc ttagcaaaaa acttagaaga attatttact aaaagaaata
    12361 tcatatgctt acaaggtgaa ataggtgcag gcaaaactac cttagcaaaa caaattgtaa
    12421 aaaatatgtt gggttcggat catgctatta gtcctacttt ttcgattttg aatatttata
    12481 ataatgataa tatttctatt tatcatatag acttatatag attaaacgat ataaaagaat
    12541 tttttgactt aggattatat gagtatttat cagataatta tgtattaata gaatggccgg
    12601 aattaatttt aaactatttg aaagattttg atttttacct tgtaaaaatt aatataatta
    12661 atgaaacaaa aagattaata gaaatatggg gatgaaaaag cattattttt atgggcctac
    12721 gggtgaattt ttacctctag aaaagagact tgaggtgaaa aataatcaaa aaaaatttgt
    12781 tataggcatt ccaaaagaga tagcctttca agagcataga gttagtttag tgccagatag
    12841 tgttaaaatg cttgtgcata atggacatga agtattggta gaagatggtg caggtgaata
    12901 tgctagattt agtaatcatg attatgctga ggtaggtgga gaaattgtaa atagccatgt
    12961 ggatattttg gctagatctg atataatagt aaaagtagct ccattattaa tgcaggaaat
    13021 acaatatatg aaaaaaaatc aaattgttat ttctgctgtc aattattatt cacaatcaaa
    13081 agaatatttc gaatcattga ttgagcgaaa tgctatagca attggttttg aatttatcaa
    13141 ggataatagt gggagatatc cagtattaat ggctatgagc gagatagtag gttatggagt
    13201 gatacaatta gcagctagat atcttagtga taataaaata agcaagggtt atctgtttgg
    13261 tggattgcca ggattaaatc ccactgaggt agtaataata ggggcaggta gagttggttt
    13321 attctctgct aaagctgcca aagcttttgg cgctttaata aaagtttttg ataataaaat
    13381 ttacaagtta cgtagcatag tttcagaatt aggtcctgat atttatactt ctgtattgca
    13441 tccagaagtt ataataaaag cactcaaaac agctaatgtt gtaatatgtg ccctgtatat
    13501 gaatgatggt gaactatcat ttttactgac cgaagatatg gtaaaagaaa tgaaagaagg
    13561 cagtataatc tttgatgtta gcatagatca agggggttgt tgtgaaacta gtgaaattac
    13621 ttacatagat gacccgattt ttgtaaaaca tggcgttatt cattattgcg tccctaatat
    13681 tgcttctaat ttccctcgta cagcagccta tgcccttagc aattttttta caccgctaat
    13741 tattgatatt gctgataatg gtggtatcaa agagtattta cgtataaatc cttatttttc
    13801 tcaaggaaca tatatactag acgaaaaaat atgtcatcca ttaatagctc aacatttcaa
    13861 ttttaaatat acaccgatac aacttttact tggtggtttt taaaaaaaaa attatctgtg
    13921 taatctgtgt aaaatgaggc aaaatgattg attagagcat aataaaaaaa aattacaaat
    13981 gccacctatt tattgattta tctctgaaaa ggtattttat tccacatttt actcctaatg
    14041 cattattaat atagaaatca tttacaaatg gtatcctaaa ttgtaattct gcaaaaactc
    14101 ctaaaccagc tacaggattt attagataag tgtatgtaat gcttgtgtag atattattta
    14161 tttgtcctag gtaattattt aagttaaatg gcgaaatttt tatattttgg tatgaagaaa
    14221 atagctgaaa aagatgtcga ttttttaaaa tatttaattg taatattatt tcttttaaat
    14281 tctctccttg ccacatacct agaggttgag ataaatgtgt gggataagta gaataatatt
    14341 cattatccat agcatgctcg gctacattat tgtattctaa tcttacatta aaaaacaagg
    14401 acgtatcttt aaattttatc ctactcaaaa taccaatttg gtatgccaac ttatttttaa
    14461 aatttttatc tattgcatca tctatattta tagtgttttg aaaataaata tcattattag
    14521 gtaaataaaa attgatagca tagccaggta aagaccaaga aggtttattc tgtaaaataa
    14581 aaggacgtag ctgtggtatt agaataaagg tataaggtga aaatctaagc tgttttttag
    14641 ctggatgaaa aattgtaaaa tctaataatg aaattgataa aaaatcataa ggattaaaag
    14701 tgaaataatt tactgaaaaa atattacgcg tagatatctc tgtgatatct actacattat
    14761 cattgtgata cgctatacca gtaatattgc ccaggataat ttttttttgg tagttaaaaa
    14821 caaaatgaat atatgggtag ggacgagcat catagcctaa tatcaaactt ctatacccat
    14881 gccctacatt caatgtattg aaaccacttt ctattgcaaa ccaattagta gcttgccatt
    14941 gaatgcgtcc caaagccaat ccgtaatcta aacctcgatt tctatatttt ttcacccaat
    15001 tatttcctgg taagaaacca atagaatcta ttaggctcat gtaatatcta ggcagatatg
    15061 cttgattttc ataaaattca ctagataaat aaagtttttt acctatttga gccatcatat
    15121 atacgcctct ggtattaaca aaattaattt ttgtattatt tgttttatct attcctattg
    15181 ataaatccaa aaaaggatta attatgattt tagctataga atcagtaagc attactaaat
    15241 tttcttcggt aagttttcta attaaccatt tattagttga aacctgttct gcaataatat
    15301 caggaaataa gcgcatataa ccattatatt ttatgttatc aaaataaagg gaactatttt
    15361 cgaaatatgt aatttgagaa tttaaagtag tgaaaataat aataaaaaaa gaaaaataaa
    15421 tagatttttt aacaaatatc attaataata aattatagtt ttacatttta gttagtaaaa
    15481 tcaaacaaat aaatcagtaa agctaaagct atcaacatcg aaaagccctt tatcagataa
    15541 ttttaaatgt ggaatcacta acaaagacat aaaagccaaa gtcataaaag gactatgcat
    15601 attgctacca tactctgttg tatatttatt caaggtttca taactgtcag cagtttttac
    15661 aacttccata tcagacataa gtcctgcaat aggtaatggt aaaatatgtg tatttttatc
    15721 acttacaaag accaaacctc ctttgtgctt ctgaataagt tcaatagctt ccactataaa
    15781 tctatcttgg gtacctatag ctacgatatt atgactatca tgagctacag atgaaccaat
    15841 agcacaattt ttgagttcaa atccatttat aaatccgatg gcaggttttg ctccactttc
    15901 atacctattt aaaactacaa tttttaagac atctctattt tggtctgaaa ttgcgaagcc
    15961 atcttcatct attgtaacag tcgttactaa tttttctgtc aatagagaac catcaaaagc
    16021 ttttattatt tgtatattat tacttgtagc tttcaacttt aaatcatcaa tactaatctt
    16081 attaatagta aaattattaa ctaattgatt aatacgtgaa gtgaaattaa ctttaccatg
    16141 atcatatacc aaatttcctt taataaaagt ttgtaaaaca ttaaaattat taaagtcatc
    16201 gattacaatt aaatctgctg gatcgtcttt ttgtaaaaat ccaacaggca agcgatagtg
    16261 ctgagctgta tttaagctag cagctcttat tgcggtgata ggatctacgc cattagcaat
    16321 ggctttacgt accaatttat ttatatgtcc tttaatgaaa tgatcaggat gcaaatcgtc
    16381 ggtgcaaaac atgattttgt ctggattttc taataacaat ggccacaaat tatcgaaatc
    16441 tttagctgct gagccttcac gaatcaagat tttcatacca agttttattt tttctcttgc
    16501 ctcctctatg ttagtacatt catgatcggt agagatgcct gcatctatat atttttttag
    16561 ttcatcgtta gataatagcg gagcatgacc atcgataggt ttgtgatgat taatagctgc
    16621 ttcaattttt tttactactt ctatatcatt atttataaca ccagggaaat tcatcatttc
    16681 tgctaaagca taaaaatcag gtttagcaag caatgaatta ataatttcag catctatcac
    16741 agaaccagaa ctttcgaatg cagtagctgg tacacaacta ggagctgtga aaaagaaatg
    16801 aaaaggaacc gttttgccat tctcaatcat aaaatcaatg cctttcacac ccattacgtt
    16861 ggctatttcg tgaggatctg ctactacacc tatggtacca tgcttcacag cttcggcagc
    16921 aaaaaacgaa ggagctacta gagaactttc tatatgtacg tgagcatcta ttaaccctgg
    16981 caaaatatag ttctcacata aaatattatc attcctttct atatttttaa ttcttccatt
    17041 tttgattttt atagtacctt tatatatttc actattaata acatctacaa tattgccact
    17101 aataatatat tttctcatat taaattattt tttacaaaaa taaaaaaatt atcaatataa
    17161 taaatacaga atatatttct atttaaaatt caataataaa gtaaaaactt aatattattt
    17221 tttgtttttg gtaattaagt tttaaattat aatttataat tttgcagaat aaaataactt
    17281 ataaattatg aataaattca ttacacctcg aaacgtagac tattctcaat ggtacttaga
    17341 tataatagag cacgcacaat tagcagaaaa ttctgtagta cgtggatgta tggtaataaa
    17401 accttatggc tttgcattat gggaaaatat acaagctacc ttagacaaga tgtttaaaga
    17461 tacggggcat attaatgcat atttcccttt atttatacca aaatcctttt ttagtaaaga
    17521 ggcggagcac gtagagggtt ttgcgagtga atgtgccatc gttacacatt atagattaaa
    17581 aaaagatcct aatagtaaag gtttgataat agacgataca gctaaattag aagaagagct
    17641 agtagtaagg cctacatctg aaactattat ctggagcact tacagaaatt ggatacaatc
    17701 atacagggat ttacctttat taataaatca atgggcaaac gtagtgagat gggagatgcg
    17761 aacaaggctt tttttaagaa ccacagaatt tttatggcag gaaggacata ctgcacatgc
    17821 tactaaggaa gaagctatgg aagaagctag attaatgctc aatatttatg cgtcttttgc
    17881 agaacaatat atggctatac cagtaataaa aggtgttaaa actgctaacg aacgctttgc
    17941 aggtgccgaa gatacatatt gcatagaagc tatgatgcaa gatggcaaag ctctacaggc
    18001 tggtacaagt cattttttag gtcaaaactt tgctaaagct tttgacgtga aatttttaga
    18061 tcgtaataat gaactccaat atgtatgggc taccagttgg ggagtatcta ctagattgat
    18121 tggtgcaatt ataatgactc atagtgatga taaaggatta gtattacctc ctaaactagc
    18181 acctactcag gtaattctaa tacctatttt tagaactgaa gaagaaaaat caaaagtttt
    18241 agaaaaatta aataatatta aaaatcaatt aattgaaaat aatataaaag ttaaattaga
    18301 tgatagagat gaattctcgc ctggatggaa atttactgag tgggaaatgc atggcgtacc
    18361 tctcagacta gctataggtc cacgtgatat tgataataat accgttgaat ttgctcgaag
    18421 ggacactggt gaaaaacata ttttacctca agataatata atagaaaatg taaaaaatta
    18481 cttagaaaat attcaaaaat ctctttttga gaaagcactt gattttagag atgctcatac
    18541 gtacattatt gatacttggg atgaatttat agaaaaaata gaacaaggta atttcatttg
    18601 ggctcattgg gatggtacca ctgagacaga attaaaaatc aaagagctca ctaaagccac
    18661 tattcgtctg ataccactat cgcctaatct agaaaatggt agctgcatac ttacaggtaa
    18721 gccctctaaa tatagagttt tattcgctcg agcatattaa tttaaatata tacattagat
    18781 aaaaacaaac aacaataagt tttgtattga ggataagtta aaattgacat catccattgt
    18841 tttactttac gtattttata ccaccatcaa ataattttta tttaatttta caaaaaaata
    18901 attatggata atttcaattt attagattat caagctctac ccaaagaaca aaaacgtaga
    18961 tttctagata atctatatca atttttatta gaaaacaact acacagccgt agattatcag
    19021 aagctaaaaa tacaaagcac agcccccatc tgtcctagat gcgaaagcga aaatgtaata
    19081 aaagctggag tacgcgacgg caaacaagtg tacaaatgca aagattgcgg ccgacaatac
    19141 cgcgaaacag ctagaacctt cgtatatcgc atgcgaaaag ctgataaaat gctcgattac
    19201 ctaaaatgca tgttagaagg caaaagtcta agagcttgcg catcggaagt aggcataagt
    19261 ctgagaacga gtttcaattg gcgacacaag atactagcag cgatcaaatc attacagggt
    19321 ggtatcagct tctcaggcat tgtagaagct gatgagctgc taatgaaata ttcagaaaaa
    19381 ggtcgcaaat acagaagccg aaaagaatac gaaaaagcaa agaaaaagaa acatcccaaa
    19441 gtagctgtgc tagtaatgac agacagggaa ggcaatttac ttttcaaaca cgtaggacgc
    19501 aaaaaagtaa ccaatagtca gataagagaa gagctatcgc atagagtgac agatagcaac
    19561 ttaatatgtg tgaagcaaaa gaaggaattc aaaaaagctg tgaaggggac caaaagtaaa
    19621 aaagttgttg taaacaaaac acagattaga cgcggtgtat acagtgtaca ggtagtcgat
    19681 aagcacataa atgatttca
//
LOCUS       DAOPRN010000036        18938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_2466_length_18938_cov_97.060690, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000036 DAOPRN010000000
VERSION     DAOPRN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18938
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2466_length_18938_cov_97.060690"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1689
                     /locus_tag="PKI65_05320"
     CDS             217..1689
                     /locus_tag="PKI65_05320"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOB77894.1"
                     /translation="MKRFILYLLFIITFANLFAQESYHKKVQAFLNYTTYYSPANGPY
                     VEFYLSIIPEGLTFTTNENYPFDHAKVSILLLLTNEDKENGITNFSKINMIIPKDSII
                     SSDNVFSHLFVMNVAPGIYKLEVKLRDEYDTLTQSIFESNLYVSTFDTISFSGIQLLH
                     SYTPVSENDDLEFVKGDYKFNYRPINILSENDSLLLFYFEIYNTAIYDTNKYFLLCVY
                     LEDLQNRQILEKYYLTKKMSTDDVIGFMGNFNVAKLPSGRYALVAEAINKNNEVVANV
                     QLPIYVDHPSIMPYEIIKTEVDFLSGINNLDTLHEYLRTLSPISSSVELEIIKKHIKS
                     TDTNEIKKFLYSFWVNRDKDNPKKAWENYLEQVKLVNNAYSTQIRKGYMTDRGRVYLR
                     YGPPNTIVRNENEPNAYPYEIWHYHHVNGENNRRFVFYNPSLTYNNYELLHSDVRGEY
                     NNPNWRQRLARKIYASGNPEEDDPDFGWGSKVNDYFDNPR"
     gene            1692..2699
                     /locus_tag="PKI65_05325"
     CDS             1692..2699
                     /locus_tag="PKI65_05325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOB77895.1"
                     /translation="MLDVAVVILNWNGLFLLQKFIPILLKYNSYGYHIYIVDNGSTDD
                     SIDWICDNYSDEIQVVVLSENFGFAKGYNEGLKQIDADYYILLNSDVAVSENWCEPLI
                     NFLQNNHDYAVVQPKILSYKNPDYFEYAGAAGGYIDKWGYPFCRGRIFNKIEKDEGQY
                     DDIVDIFWASGACFVVRAEVFKKLNGFDDRFFAHMEEIDLCWRIWRNGYKVAAVPLSA
                     VYHLGGASLDYNDPRKLFLNIRNNHLMLYKNLTSDIFKKISHVRHMIEYLMLFFYFCT
                     GKISNAKAILSAYKDFNDLKKYYKPANEASILKLLYPRSILVDYYLKNKKKFSKLKWK
                     I"
     gene            complement(2838..3416)
                     /locus_tag="PKI65_05330"
     CDS             complement(2838..3416)
                     /locus_tag="PKI65_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOB77896.1"
                     /translation="MKKNKTRNHILKKAAEIFAIKGIENTTIEDISNHLGKAKSFIYY
                     YFEDKDSLYREVLEHELDTFKKKVYEKLNSTVDPISKLRMYFELRIQKTKELVNLFSF
                     KKQEIEYKIPFIKEFRAKYDKEEANIIKQILVEGAERNIFYLTDLTNTTIAIATALKI
                     SELPYLLPEYIENYDEMMDSLIDIVLYGIVKR"
     gene            complement(3532..3834)
                     /locus_tag="PKI65_05335"
     CDS             complement(3532..3834)
                     /locus_tag="PKI65_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77897.1"
                     /translation="MKHINIHCDVAQANEIIEILEKCNISGFQIIDKVKIKNRKGNPR
                     MGNAVWPGYNVLIMTQIREEEKITVLINKIKDYNANVSHTDQLITLEGWNIDTFIF"
     gene            complement(3831..6929)
                     /locus_tag="PKI65_05340"
     CDS             complement(3831..6929)
                     /locus_tag="PKI65_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOB77898.1"
                     /translation="MKITDISVKNPVAIYMFFAGIILFGLLAIFRLPRDLFPDIEYPT
                     LTVVTVYPGASAEQVEKEVTNPLEVILASTENIKNIQSFSKDNVSTISLQFDWGSDIT
                     EAAANARDLIELVKYQLPSEAQRPIIMKINSSVMPVVILGLQTTNTNVDISDLIEKVI
                     SPRLQHIDGVGTVISIAQPETEIAVEIDPLKAEKYGLTIDYIANLIKSFNLNVPAGSL
                     KTGVWDLNVTVHGSVPSIEAIYHLPITTYLGQVIRLKDIAIVKKQYKQKDEIARTHQE
                     RGTALMVQKQSQANALQVYEAVKKEIACLQLPPETNIYEVFNTTEIIKGSINNITETL
                     LWGGLCVIIVVWLFLRAWRSSLIISLAIPFSLLIALITMMVAGFTINVFTLMSMVVAI
                     GMIIDNNIVVVENITKYIDRGIRKKEASIFATGEMGQAITASTFTTIAVFFPLIFAGG
                     IVGMFFQQFAIIGATTMLGSLLIALTLTPTASSQLFIEKSKQKKRLKIYEKTESWYLK
                     LEDNYGKLINWSLHNKGIIIIIMVAFILLAFILGKWFIGTDYIPNVDGGDLITVIQMP
                     DGYSVEETEKVAAKVEKFIEDNVPEILYGFTVAGQTDQGLLSSVGFKENKNLATIVYH
                     LVPPNKRKRSAEEIADMIVIELKKIPEIEQFNVVGGSILVSALLGSGSPVEIVITGTD
                     LETLRKYAKIVEDSLKNIPFLKNIQNNIVNTKPEFQVTLDKDRMANLNINPAIAAMQV
                     RQSLYGIEAGKYEQQGKEIPINIKYMRDELKSVNDVLNIRISSLLGSSYPLREVAEVQ
                     LNNGYIEIMHDNQQRVAVVGADINTNVDLGTVTKEIQKIISSIDTYDEVIIKQKGQAE
                     EQAKSFKDLGLILVISVLLVYMIMAAQFGNLLQPFIIFLSSPFAVVGSIIAFYLTGTT
                     LNLISFVAIILLMGVVVNNGIVLVDYTNMLIKRGYSLNDAIIAAGISRLRPVLMTTFT
                     TIMGILPMAINRGLLHEIWSPFGITTIGGLFFATLITLIWIPLLYSLFYSHFEHKKSL
                     YI"
     gene            complement(6943..8001)
                     /locus_tag="PKI65_05345"
     CDS             complement(6943..8001)
                     /locus_tag="PKI65_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOB77899.1"
                     /translation="MNKIITLLSISTLLVFCSCKKTNQVINTTDQDDILKVQIYQVKK
                     EDVPLTLTYLGNFKPYKEAVLGTSIPGKIEKIYYDEGSTVKQGDLLIQMSSELLLQAE
                     AEYRTLEKDYQRVKNLAEKQSISQQDYDHVKAKYDAATARYNLLKSNTEIRAPFDGII
                     AKHLAHEGENYFFSASLEIGSTFTSGIVQLVKINPIYFEFEISEKDLTFIKNKTPVRA
                     IANIYPKDTIIGYVDFISPTLNPTKATATIRIKINNATMKYKPGMTGKAFINVIRHDI
                     MLIPLKGIIKELNEDSYFVYTVDKFNKINKKNITILEYIGQYVAVEGLNIDDKVVISS
                     KKNLKEGLSVNIIEIIKN"
     gene            complement(8207..9673)
                     /locus_tag="PKI65_05350"
     CDS             complement(8207..9673)
                     /locus_tag="PKI65_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOB77900.1"
                     /translation="MKSKTLFLFLMITISLSAQEMLTLQDCKTIAIRENLSLSIDSIK
                     IKSAIHTKRAAFANYFPDISFAGTFLHLNKQFQLFENDILLPVVPHQAIDLNTMHINQ
                     ESFSNPMVALLSFAIDPSTLQPLTDVNGNPVFRNYAWLPADQSKFGLKDNYLFNFSLK
                     QPIFVGGKIFYSNKMANNSVEIAKYKTEMDKQNLLYEIEDMYYKTWIIDKKCKLAHKY
                     LELLQEIEKDIKNYIDEGIATTNQLLMIQTKINQAQVNLFKAENGLTIQKQLLNQKMG
                     RDIHQDFSLVDTIDFDINIIHLIINENLDINNRPEIKLLEQKVELSQNMLRVTQADML
                     PQIGFMANYFFVNPNPYKGFANEFGDDYTFGVSIQIPIFHALERYNKRQAALLQYKAA
                     QIEMNDIKNLISIETSYTTNQLKEQLMQLNATNKNVELARENLRVQTDLFHEGILKLS
                     DLTEAQTMWLQAETDYYEAYALVLAYYAKLKKDYGIID"
     gene            9858..10079
                     /gene="tatA"
                     /locus_tag="PKI65_05355"
     CDS             9858..10079
                     /gene="tatA"
                     /locus_tag="PKI65_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOB77901.1"
                     /translation="MVFIGVITSTEWIIILVIVIILFGGRKIPELMHSLGRGVSEFKK
                     GKEGIEDDLNTNKKNRTEKTDKTDNNPAG"
     gene            10088..10864
                     /gene="tatC"
                     /locus_tag="PKI65_05360"
     CDS             10088..10864
                     /gene="tatC"
                     /locus_tag="PKI65_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOB77902.1"
                     /translation="MEFWDHIEELRKRFIRIIIVIIVLMIIVFSFKSFLFDTLLFGPL
                     DPNFITYKFICQIFPKSGICTNPFYINLQNLTISGQFIKHISISFLVSLFLAFPYILW
                     ELWMFVKPGLYDYEKKSAIKILINALLLFIVGALFSYFIIVPLTINFFFAYQISPMVI
                     NQFTLDSYFTTFTTLLVLIGLVFELPIILNFLIKFNIIKLSTLKKQRKVIFIVILIIA
                     AYITPGGDPISLTIMTAPLYLLFELSIKIAAKSEKKQKTI"
     gene            complement(10976..12199)
                     /locus_tag="PKI65_05365"
     CDS             complement(10976..12199)
                     /locus_tag="PKI65_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77903.1"
                     /translation="MKKYICALFIIFTYTLYSQQIPFNVAFENLNFNNKQNIYFTESD
                     TFIYNLKNGVIDDDFIYSDTNGVKVSGRYKYCHPSGKWVINYLDNKIKLRFTNDGIIH
                     FNRVRKDKHNVFAGFGKLNISYTISHNTIIDTIIAICPIRWGKISGELRETYTDSSTR
                     AIYNYKKGKYHGIQTFFYDNKGYWQIEYKNGKPASNKTFYDTNGNEFRSIQLKELHQN
                     SNVIAFYDPTDIIYNTKILLTLDSNFREYPLLSEKIIDSINKMYYYRNIITYKDYDLR
                     NEDWGAHNRPNIWDITTDTILTKKNIIGIALTGNLIILHQDFLYRLQPLTAAYVVCYD
                     FQNEKHYALTPWFYLPELMQRNLILPYGSILFYPYKIVNFPIDNLLDKDIIKNHSQAL
                     IPVSYIRFLKKLFYL"
     gene            complement(12245..13078)
                     /gene="lgt"
                     /locus_tag="PKI65_05370"
     CDS             complement(12245..13078)
                     /gene="lgt"
                     /locus_tag="PKI65_05370"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOB77904.1"
                     /translation="MSFLAYVVWNVDPVLLRLGPLEIRYYGVLFALGFLVGYYLFRYF
                     FKTEKVPLSEIDRLFWYVILGAVIGARLGHVLFYEPDYYFKHPLEILAIWHGGLASHG
                     GAIGLLIALYLYSRKSFNKDYLYILDRMAIPTALAGSFIRLGNLFNSEIYGRPTELPW
                     GFIFVRDGQTVPCHPTQIYESLAYLLIFIFLFSMYKKYKGQIPRGLAIGWMLLLTFIA
                     RFLIEFIKLPQVGFEMKLIQTIGLNMGQLLSLPFIIAGIWLIIKANKSKKVVRQPQKT
                     K"
     gene            complement(13372..14316)
                     /locus_tag="PKI65_05375"
     CDS             complement(13372..14316)
                     /locus_tag="PKI65_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77905.1"
                     /translation="MRISGFTFCRNADKLYYPIKQSILSILPIVDEFIINIGDCDDDD
                     KTLEVVSSINSPKIKIIQSKWDLDKYPNGTENAHQTDIAKSACSGDWLFYLQADEVVH
                     EKYLENIIKRCEELIDNDEIEGLLFDYIHFYGDYWHYQNGHGWYKKEIRIIRNDPDIH
                     SWESAQSFRRIPNFDGLHYRQQKDTYKLKVAKVDAKIYHYGWVRPPQLMQKKKIALDT
                     IHKGVNNLTKDENQHIETFSYGPLGVLPIFKETHPAVMNEWIKKFNWQDQLNYSKKAD
                     NLYKKPSKRHRILTKIEKLLFNNKISIGEFKNYKLIKT"
     gene            14687..15202
                     /locus_tag="PKI65_05380"
     CDS             14687..15202
                     /locus_tag="PKI65_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOB77906.1"
                     /translation="MALIKSVKGLSPKWGSECWIAENATIVGEVVMGNKCTVWFNAVI
                     RGDVHYIHIGNNVNIQDQVVIHCTYQTSPVEIGNNVSIGHGAIVHGCKIGNNVLIGMG
                     AIIMDDVIIEDNCIIAAGAIITKNTHVSSYSVMAGIPAKKIKNLTKELFQKEVVDIAN
                     HYPIYAKWYDI"
     gene            15769..16800
                     /gene="porQ"
                     /locus_tag="PKI65_05385"
     CDS             15769..16800
                     /gene="porQ"
                     /locus_tag="PKI65_05385"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOB77907.1"
                     /translation="MKNLKIYISTYVCLLFITLCNGQSAYSFLDFGNNARSNALGSML
                     YSVYESDVLSYYHNPGFINDSDAYSIGFNYQTYAAHISQYTLNFNPYIRNFGTLAFTI
                     KYLDYGNFKGYDWTGDPTNDFSVYSTVFAVGYGKQLFDKFNIGINLKGAYMRAEKYGF
                     ALASDVALNYYDTINKLSLSLFLRNIGHEVSQSFGDEKNSSLPFNLAFAMSKRFKNAP
                     FRFHLYFHDIHNWKLRFTDTTSLQIDPFTNEYIEPKKFAKITDEFMRHFAFGIEIIPT
                     KFLSLMMGYNYQNRQEMTIESRKGLIGFSFGVGLYTRRFSFIYSYNISHLAYGPNNFS
                     LIIPLKIYF"
     gene            16824..17519
                     /locus_tag="PKI65_05390"
     CDS             16824..17519
                     /locus_tag="PKI65_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77908.1"
                     /translation="MDRNPKVKTLKNLASYLEKKANLKQDVYDNTKKAFDLIKQEAMF
                     LLKDIRGNMLNKKRVIPLEIIERTDFEFEIKFAGDILAFFMHTNAFMLPPYHPATQNR
                     YAQENKENTFVGIINIFNFLADSFKYNRYNDVGFLIGRIYVNKDNHFYIDGRSEIQNI
                     PIQFETQKISAKEMRNVLINCILSTIDFDLIAPPLEYYEAIPVKTFIDAIESYPIKTS
                     KLVGFKVNSFQNK"
     gene            complement(17548..18423)
                     /gene="sucD"
                     /locus_tag="PKI65_05395"
     CDS             complement(17548..18423)
                     /gene="sucD"
                     /locus_tag="PKI65_05395"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOB77909.1"
                     /translation="MSILVNYKSRVLVQGITGKEGSFHTQQMIEYGTKVVAGVTPKKG
                     GTKFNDIPIFNTVDKAIMETGANVSIIFVPASNAADAIVEAASAGIELIICITERIPV
                     QDMIKVKAFLKNTSTRLIGPNCPGVITPGEAKVGIMPGFIHKKGNIGIASRSGTLSYE
                     VVYQLTRLGLGQSSMVGIGGDPILGSSMKEIVELFMNDDDTDTIVIIGEIGGGTENEV
                     ADYVKDNKVKPVFAFISGVTAPKGRTMGHAGAIIGGKDDTAQAKKDYLRSCGINIIDS
                     PADIGRTVFNFIKNK"
     gene            complement(18531..18713)
                     /locus_tag="PKI65_05400"
     CDS             complement(18531..18713)
                     /locus_tag="PKI65_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77910.1"
                     /translation="MRRFRGVATKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
BASE COUNT         6629 a         2711 c         2623 g         6975 t
ORIGIN      
        1 actagataga caaaaaaagg ggggcatcat ttatttgatg ccccctcttt ttttttatca
       61 gtaaaggata ttttaataac aacaagacaa tgtaaccaaa ttaatataaa tacatcatag
      121 gtatgcgtta taatgtgcat ttgcaatatc aaattttgta aattatatta aaattatttt
      181 gtaattttat aaaaatatta ttttagtata aaaataatga aacgatttat tttatattta
      241 ttatttataa taacatttgc aaatttattt gctcaagaat cataccataa aaaagtgcaa
      301 gcttttttaa attatactac ttattattct cctgctaatg gtccttatgt agagttttat
      361 ttatctataa tacctgaggg cttaacattt acaactaatg aaaattatcc ttttgatcac
      421 gctaaagtat cgattttgtt actgttaaca aatgaagata aagagaatgg tattactaat
      481 ttttcgaaaa taaacatgat aatacccaaa gatagtataa taagtagtga taatgtattt
      541 tcacatttat ttgtgatgaa tgtagctcct ggtatataca aattagaagt aaagctgcgt
      601 gatgaatatg atacactcac acaatctatt tttgaaagca atctttatgt atcgacattt
      661 gatacaattt cattttcggg gatacaactt ttacatagtt atacacctgt aagcgaaaat
      721 gatgatttag aatttgtaaa aggtgattat aaatttaatt accgtcctat aaatatatta
      781 agcgaaaacg attctttact tttattttat tttgagatat ataatacagc tatttatgat
      841 actaataaat attttttgtt atgtgtatat ttagaggatt tacaaaatag acaaatttta
      901 gaaaaatatt atttgacaaa aaaaatgtct actgacgatg taataggatt tatgggtaat
      961 tttaatgtag ctaagctccc ttctggcaga tatgctctag tagctgaagc aataaataaa
     1021 aacaatgaag ttgtagctaa tgttcaatta cctatctatg tagatcatcc atctattatg
     1081 ccttatgaaa tcataaaaac tgaagtagat tttttgtctg gtattaataa tttagatact
     1141 cttcatgagt atttgagaac attatctcct attagcagct cagtagagct agaaataata
     1201 aaaaaacata taaaatctac tgacactaat gaaataaaga aatttcttta ttcattttgg
     1261 gtaaatcgag ataaagataa tcctaaaaaa gcatgggaaa attatctaga acaagtaaaa
     1321 ttggtcaata atgcgtacag tacacaaatc agaaagggat acatgacaga tagaggtcgt
     1381 gtttatctac gatatggtcc accaaataca atagtacgta atgaaaatga acccaatgct
     1441 tatccttatg aaatatggca ttaccatcat gtaaatggag aaaataatag aagatttgta
     1501 ttttacaatc cttcgcttac atacaataat tatgaactac tgcatagtga tgtgagaggt
     1561 gaatataata atccaaattg gagacaaaga ttagctagaa aaatatatgc ctctggtaat
     1621 cccgaagaag atgatcccga tttcggatgg ggtagcaaag taaatgatta ttttgataat
     1681 ccacgctaat catgcttgat gtcgctgtag tcatactaaa ctggaatgga ctttttttat
     1741 tacaaaaatt tattcctatt ttacttaagt ataattctta tggttatcat atatatattg
     1801 tagataatgg atccactgat gatagcattg attggatttg tgataattat tcagatgaaa
     1861 ttcaagtagt agttcttagt gaaaattttg gttttgcaaa aggatataat gagggattaa
     1921 agcagataga tgccgattat tatattttat taaattcaga tgtagctgtg tctgaaaatt
     1981 ggtgtgaacc tttaataaat tttttacaga ataatcatga ctatgctgtg gtgcaaccta
     2041 aaatactaag ttataagaat ccagattatt tcgaatatgc tggagccgcg ggaggctata
     2101 tagataaatg gggttatcct ttttgcaggg ggcgtatttt taataaaata gagaaagatg
     2161 agggacaata tgatgatata gtagatattt tctgggcatc gggtgcttgc tttgtagtta
     2221 gagctgaagt gtttaaaaaa ttaaatggtt tcgacgatag attttttgct catatggaag
     2281 agatagatct ctgttggagg atatggcgta atggttataa agtagctgct gttcctctaa
     2341 gtgcagttta tcatttaggt ggtgctagct tagactacaa tgacccaaga aaattgtttc
     2401 taaatatcag aaataatcat ttgatgctat acaaaaatct aacatctgat atttttaaaa
     2461 aaatttcaca cgtaagacat atgatagaat atctaatgtt gtttttttat ttttgtactg
     2521 gtaaaatatc taatgccaaa gctattttaa gtgcttataa agattttaat gacttaaaaa
     2581 aatattataa acctgctaat gaagcatcta tattaaaatt attgtatccg aggtctattt
     2641 tggtagatta ttatttaaaa aataaaaaaa aatttagtaa attgaagtgg aaaatttaat
     2701 aaattcagaa tgaatatgcg aggttgagaa gtgagagagt gggggataat tgaaagttag
     2761 agtttgaaat ttctataaaa tataaaattt tttattgtta gtttaaattt ttattcaatt
     2821 tagtataaaa taaatcttca acgttttact atgccatata gtactatatc tattaggctg
     2881 tccatcattt catcatagtt ttctatgtat tctggtaata agtaagggag ttcgctaatt
     2941 ttcagagctg ttgctattgc tatagtagtg ttagtcaaat ctgttaaata aaaaatattg
     3001 cgttcggctc cttcgactaa aatttgtttg ataatatttg cctcttcctt atcatattta
     3061 gcgcgaaatt ccttaataaa tggtatttta tattctattt cttgtttttt aaaagaaaat
     3121 agatttacta attctttagt tttttgtata cgcaattcga aatacattct aagcttagaa
     3181 atagggtcta ctgtagaatt taatttttca taaacctttt ttttgaatgt atcaagttca
     3241 tgctctagca cttctctata taaagaatct ttatcttcga aataataata aataaagctt
     3301 ttagctttac ctaaatggtt agaaatatcc tcgatggttg tattttctat acctttaata
     3361 gcaaaaatct ccgctgcttt ttttagtata tgatttcttg ttttattttt tttcataatt
     3421 taattgattg tccaatgata aaaaattact atatcttaat tttaaattta ctaccattta
     3481 gcaagtagat acagctaata atgcaaaaca aaattttgta aagcataatt ctcaaaatat
     3541 aaatgtgtca atattccagc cttcgagtgt aatgagttga tctgtatgcg aaacattggc
     3601 attataatct ttgattttat taatcaaaac tgtaattttt tcttcttctc ttatctgagt
     3661 cattattagt acattatatc caggccatac agcattaccc atgcgaggat tgccttttct
     3721 atttttaatt tttactttat caataatctg aaaaccactg atattacatt tttctaaaat
     3781 ttcgattatt tcattagctt gagctacatc gcaatgaata tttatatgtt tcatatatat
     3841 aaagattttt tatgttcaaa atgagaataa aataaagaat aaagtagagg gatccaaata
     3901 agagtaatga gagttgcaaa aaataaacct ccgatagtag taataccaaa gggactccat
     3961 atttcatgta gtaggcctct attaatagcc ataggtaaaa tccccataat agttgtgaat
     4021 gtggtcatta atactggtcg tagtcgagat atacctgcag ctataatagc atcattaaga
     4081 gaataaccgc gtttgataag catattagta taatctacta aaactatgcc attatttact
     4141 actacaccca tgagcaaaat aatagctaca aaagatatca aatttaatgt agtacctgtt
     4201 aaataaaaag ctataataga gccaaccacg gcaaaaggtg aggaaagaaa aataataaaa
     4261 ggttgcaata aattaccaaa ttgagctgcc atgatcatgt atacaagtaa aacgctaata
     4321 accaaaatca atccaagatc tttgaaactt ttagcttgtt cctctgcttg acctttttgt
     4381 ttaataatta cttcatcata ggtatcaata cttgatatta ttttttgtat ttctttagta
     4441 accgtaccaa gatcgacgtt agtatttata tctgcgccta ctacagctac acgttgttga
     4501 ttatcatgca tgatctctat gtatccatta tttagttgta cttctgcgac ttctcgtaat
     4561 ggataactac taccaagcaa agaagagata cgtatattta gtacatcatt tacagatttt
     4621 aattcatctc tcatatattt gatgttaata ggaatttctt tgccttgctg ttcatatttt
     4681 ccagcttcaa taccataaag actttgtcgc acttgcatag cagctatagc tgggttaata
     4741 ttaagattgg ccatacgatc tttatctaag gttacttgaa attctggttt agtatttaca
     4801 atattatttt gtatattttt caaaaaagga atatttttta aagaatcttc gacaatttta
     4861 gcatatttac gaagcgtttc caagtctgta ccagttatta ctatctctac agggctgcca
     4921 ctgcctaaaa gagcagatac caatatggac cctcctacta cattaaattg ctcaatttct
     4981 ggtatttttt tcaattctat cactatcata tcagctatct cttcagccga gcgtttacgt
     5041 ttatttggtg gtactaaatg atatacgata gtagccaaat ttttattttc tttgaaacct
     5101 acggatgaga gtaagccctg atctgtttga cctgctacag tgaagccata taatatttca
     5161 ggaacattat cttcgataaa tttttcaact ttagcagcta ctttttcagt ttcttctacg
     5221 ctgtagccat cgggcatttg tatgactgtt atcaaatcgc caccatctac atttggaata
     5281 taatctgtac caataaacca tttacctaaa ataaaagcta gtaaaataaa agcaaccatt
     5341 ataataatta tgataccctt attatgtaag ctccaattaa ttaatttacc ataattatct
     5401 tcgagcttca gataccaact ttcagttttt tcatatattt ttaaacgttt cttttgtttt
     5461 gatttttcaa taaataattg actactagct gtaggagtaa gagtaagagc tattaaaagc
     5521 gagcccaaca tagtggtggc acctatgata gcgaattgtt gaaaaaacat acctactata
     5581 ccaccagcaa aaatcaatgg aaaaaataca gcaatagttg taaatgtaga agcagtaata
     5641 gcctgtccca tctcaccagt agcaaagata gaagcttctt tttttctaat acctctatcg
     5701 atgtatttag ttatattctc taccactacg atgttattat ctattatcat acctatagct
     5761 actaccatag acattaatgt gaaaacattt atagtaaaac cagccaccat catagtaata
     5821 agtgctatta ataaagaaaa gggtatagct aaactaatta tcaaactaga tctccaagct
     5881 ctcaaaaaca accatactac tattatcaca catagcccac cccataaaag agtttcagta
     5941 atattgttta tagatccttt aataatttca gttgtattga atacttcata aatattagtc
     6001 tcagggggta gttgcaaaca agcaatctct ttcttaacag cttcgtatac ttgcaaagca
     6061 ttagcttgag attgcttttg taccataagg gcagtacccc tttcttgatg tgtgcgagct
     6121 atctcatctt tttgtttata ttgctttttc acaatagcta tatcttttag ccttatcact
     6181 tgcccgaggt aagtagtaat aggcagatga tatatagctt cgatagaagg tacagagcca
     6241 tgtacagtaa cattcagatc ccatacgcca gttttaagtg aaccagcagg tacgtttaga
     6301 ttaaaacttt taattaaatt agcaatataa tcgattgtta atccatattt ttcggctttt
     6361 aatggatcta tctctacagc tatttcagtt tcaggttgtg ctatcgaaat gacagtacct
     6421 acaccatcta tatgctgtag cctaggagaa atgacttttt cgattagatc actaatatct
     6481 acatttgtat ttgttgtttg cagacctaat atcactaccg gcatgacaga tgaatttatc
     6541 ttcattatta ttgggcgttg agcttcactt ggtagttgat attttactaa ttctattaaa
     6601 tctcttgcat ttgcagctgc ttcagtgata tctgatcccc aatcaaattg taaagaaata
     6661 gtagatacat tatcttttga aaaactttga atatttttta tgttttctgt gcttgctaat
     6721 attacttcta atggattagt tacttctttt tctacttgct cagcactagc accaggataa
     6781 actgtaacta cagtaagtgt ggggtattca atatctggaa acagatctcg tggtaatcga
     6841 aatatagcta ataagccaaa taaaattata cctgcaaaaa acatataaat agctacagga
     6901 ttttttaccg atatatctgt gattttcata gtaatattta ttttaatttt ttataatttc
     6961 aatgatattt acactcaatc cttctttaag atttttttta cttgagatta ccaccttatc
     7021 atcaatattt aagccttcta cagccacata ttgtcctatg tattctaaaa tagtaatatt
     7081 ttttttgttt attttgttaa atttatcaac tgtatacaca aagtagctat cttcatttaa
     7141 ttctttaatt atacctttta atggtattag cattatgtca tgtctaataa cattaataaa
     7201 tgctttacct gtcatacctg gtttgtattt catagtagca ttatttattt taatacgtat
     7261 ggtagctgta gctttagtgg gatttagagt aggactgata aaatccacat aacctattat
     7321 agtatcttta ggataaatat tagctatagc tctaacggga gttttatttt taataaaagt
     7381 taaatctttt tcacttatct caaattcgaa ataaatagga ttaattttta ctaattgcac
     7441 tatgccagaa gtgaaagtag aaccaatttc taatgaagca gaaaagaaat aattttcacc
     7501 ttcgtgagcc aaatgtttag caataatacc atcaaaaggg gcgcgtattt ctgtattgga
     7561 ttttaataaa ttgtatctag ctgtcgctgc atcatatttg gcctttacat gatcataatc
     7621 ttgctgactt atagattgct tttctgctaa atttttcact ctttgataat ctttttctaa
     7681 tgttctatat tcagcctctg cttgtaaaag caattcagat gacatttgta ttaaaagatc
     7741 gccttgtttc acagtactac cttcatcata ataaattttt tcaattttac caggtataga
     7801 agtgcctaat acagcttctt tatatggttt aaaattacca agatatgtta gtgtcaatgg
     7861 tacatcttct ttttttactt gatatatttg tacttttaat atatcatctt gatctgttgt
     7921 gtttataact tgatttgttt ttttacaact acaaaataca agtagtgtac taatggataa
     7981 taatgtgata attttattca tatttttatt ttttttggaa aaacaaactg attaattata
     8041 aacatatgcc ttgatattaa agatatttgg cgtattagat aatatataca caatggtata
     8101 tgccagcact gacttatatt tatacgatat aatattagca tggtaggact ttttatgaat
     8161 ttattttcaa aaatattaca tatcataatt atcagatttt caattattaa tcaataatac
     8221 cataatcttt ttttaattta gcataataag caagtactaa ggcatatgct tcataataat
     8281 cagtttcagc ttgtaaccac atagtttgag cttctgtgag atcagacaat ttgagtatac
     8341 cttcatgaaa aagatcagtt tgcactctta gattttctcg agctaattct acatttttat
     8401 tagttgcatt taattgcatt agttgctctt ttaattgatt agtagtatat gatgtttcta
     8461 tagaaataag atttttaata tcattcattt ctatttgtgc agctttgtat tgcaaaagtg
     8521 cggcttgtct tttattataa cgttctagtg catgaaatat gggaatttgt atagaaactc
     8581 caaatgtata atcgtcacca aactcattag caaagccctt gtatggatta gggtttacaa
     8641 aaaaataatt tgccataaat ccaatctgag ggagcatatc agcctgtgta acacgcaaca
     8701 tattttgact taattctact ttttgttcta ataatttaat ttctggccta ttattaatat
     8761 ctaagttttc attaataatt aaatgaataa tatttatatc aaagtcgatg gtatctacaa
     8821 gtgaaaaatc ttgatgtata tccctcccca ttttttgatt taataattgt ttttgtatag
     8881 tcaaaccatt ttcggctttg aatagattga cttgagcttg atttattttt gtttgtatca
     8941 tcaatagttg attagtagta gctataccct catctatgta gttttttata tctttttcta
     9001 tttcttgtaa aagctctaga tatttatgtg ctaacttaca ttttttatct attatccatg
     9061 ttttataata catatcttct atttcatata ataaattttg tttatccatt tcagttttat
     9121 atttagctat ttctacagaa ttgtttgcca ttttgttaga ataaaaaatt ttgccaccga
     9181 caaaaattgg ttgttttaat gaaaaattaa aaagataatt gtcttttaac ccaaatttag
     9241 attgatctgc tggtaaccaa gcataatttc taaatacggg attaccatta acatcagtaa
     9301 gtggttgtaa agtagatggg tctatagcaa agctaagtaa agctaccata ggattagaaa
     9361 aactttcttg atttatatgc atagtattga gatctatggc ttgatgtggt acaactggaa
     9421 gcaaaatatc attttcgaac aattgaaatt gtttattcaa atgtaaaaat gttccagcaa
     9481 agctaatatc agggaaataa ttagcaaaag cggctctttt tgtatgtata gcagatttta
     9541 tcttaatgct atcaattgat aatgataaat tttctcttat tgcaatagtt ttacaatctt
     9601 gtaaagttag catctcctgt gcagagagcg atattgtaat cattaaaaat aaaaacaatg
     9661 ttttgctttt catatttttt gaacaaaagt aaaaataatt tcaaatataa aaaaatattt
     9721 tttataataa ataaattttt tagtatatga gtaatgaaaa atttctttat caaaaaaaat
     9781 acacaatgat ttttatcaaa ttaaataatt atacatatca aaaaaaagat ttaatttagc
     9841 attaaaaaga ataaagaatg gtatttattg gggtaatcac atctacagaa tggataataa
     9901 tcttagtaat agttattata ttatttggtg gtcgcaaaat tcctgagctt atgcattcat
     9961 taggcagggg agtaagtgaa tttaaaaaag gtaaagaagg gatagaagat gacctaaata
    10021 caaataaaaa aaatagaaca gaaaaaacag ataaaacaga taataatcct gctggatagc
    10081 tataccgatg gagttttggg atcatataga agagttacgt aaaagattta taaggattat
    10141 aatagttatc atcgtattaa tgataattgt ttttagtttt aaatcttttt tatttgacac
    10201 attactcttt ggtcctttgg atcctaattt tattacatac aaatttattt gtcaaatttt
    10261 cccaaaatct ggtatttgta ctaatccatt ttatattaat ttgcaaaatt taactataag
    10321 tgggcaattt atcaagcata tttctatttc atttctagta agtttatttt tagcatttcc
    10381 gtatatatta tgggaattat ggatgtttgt aaaaccaggt ctatatgatt atgaaaaaaa
    10441 aagtgctatt aaaatattaa ttaatgcttt attacttttt atagttggag cactttttag
    10501 ctattttatc atcgttcctc tcactataaa ttttttcttt gcataccaga taagcccaat
    10561 ggttataaat caatttacat tagactctta ttttacaacc tttactactt tattagtatt
    10621 aataggttta gtttttgaat tacctattat acttaatttt cttataaaat ttaatattat
    10681 caaactttct acacttaaaa aacaaaggaa ggtgattttt attgtgatat taatcatagc
    10741 agcttatata accccaggtg gggatccaat atcattaact ataatgacag ctccattata
    10801 tttgttattt gagcttagta taaaaatagc tgctaaaagt gaaaaaaagc aaaaaacaat
    10861 ttaaatacat tttcatttca atatcattat aattgaaaat atatgaataa taagttgaaa
    10921 ctgctttaat agttaacaat ttgcattttt attaatttta ttatgtaatc tgattttata
    10981 aatagaataa tttttttaag aatctaatat aagatactgg tattagagct tggctatgat
    11041 ttttaattat atctttatct aataaattgt caatagggaa atttacaatt ttgtatggat
    11101 aaaataatat ggatccatat ggtaaaatta aatttctttg catcaattca ggtaaataaa
    11161 accaaggtgt taaagcataa tgtttttcat tctgaaaatc gtaacacact acataagctg
    11221 cagtgagtgg ctgtagccta tatagaaaat cttgatgcaa aattatgaga ttacctgtta
    11281 gtgctatacc aataatattt ttttttgtga ggatagtatc tgtagttata tcccatatat
    11341 ttggtctgtt gtgagctccc cagtcctcat tccttaggtc ataatctttg taagtaataa
    11401 tatttctata ataatacatt ttattgatag aatctattat tttttcagat aaaagtggat
    11461 attctctaaa attgctatct aatgttaata aaattttagt attataaata atatcagtag
    11521 gatcataaaa agcaataaca ttagaatttt gatgcaactc ttttagttga atacttctaa
    11581 attcattacc attagtatca taaaaagttt tattggatgc aggtttgcca tttttgtatt
    11641 ctatttgcca gtagccttta ttgtcataaa aaaatgtttg tataccgtga tactttcctt
    11701 ttttataatt ataaatagct cgtgtagagc tatcagtata tgtttctcta agctctccag
    11761 aaatttttcc ccatcgtatc ggacatatag caattatagt atctataatt gtattatgag
    11821 atattgtata cgaaatattt aacttaccaa aaccagcaaa aacattatgt ttgtcttttc
    11881 tcactctatt aaaatgaata attccatcat tagtgaatcg taatttgatt ttattatcta
    11941 aataattaat aacccactta cccgatgggt ggcaatactt atatctgcca gagactttca
    12001 ctccattagt atcagaataa ataaaatcgt catcaattac accatttttt aaattataaa
    12061 taaaagtatc actttcagtg aaataaatat tttgtttatt attaaaattc aaattttcga
    12121 aagcaacatt aaatggtatc tgttgagaat ataatgtata agtaaaaatg ataaataaag
    12181 cgcagatgta ttttttcata aacaattttt atataaaatg taaaaaaatc attaaataag
    12241 atatttattt tgttttttgg ggctggcgta ctactttttt agatttatta gctttaataa
    12301 taagccatat tccagcaata ataaatggta aactaagtaa ttgccccatg tttagtccga
    12361 tagtttgtat tagtttcatt tcaaatccta cttgaggtag tttgataaat tctatgagaa
    12421 atctagcaat aaatgtaagt aatagcatcc atccaatagc taatccgcgt ggtatctgtc
    12481 ctttgtattt tttatacatt gaaaaaagaa agataaaaat aagcaaataa gctaatgact
    12541 cataaatctg agtaggatga catggtactg tctgtccatc tctaacaaag ataaatcccc
    12601 atggcagctc agtaggtcta ccataaattt cactgttaaa taaattacct aatctgataa
    12661 aactaccagc taaggctgta ggtatagcca ttctatctaa tatataaaga taatctttat
    12721 taaaagattt acgtgagtac aaatatagag ctattaataa tccaatagca ccgccatgac
    12781 tagctaaccc tccatgccaa atagctaaaa tctctaatgg atgtttaaaa taataatcag
    12841 gctcataaaa tagtacatga ccaagtctgg cacctatcac tgctcctaat attacatacc
    12901 aaaacaatct atctatctcg cttagaggca ccttctcagt tttgaaaaaa tatctaaata
    12961 aataataacc tactaaaaat cctaaagcaa ataaaacgcc ataatatctg atttctaaag
    13021 gaccaagacg cagtaataca ggatctacat tccaaacaac ataggctaaa aaagacataa
    13081 tttatttttt gcaaaaataa gtaaaatgaa taattaaaaa aactaatgtt tgagtgatat
    13141 aaaatacaaa attaaaaagg aataaatcgt agagagtaaa ttaatatagt tttgaaaaaa
    13201 tttaaaattc taaataatag atttgcaaca caaaattttt tcatcaaaaa ttttaactta
    13261 ctctacagtt taatattgat atttgtttgt gaaatttatt aatgtgatta ttgtaaaatt
    13321 atttttaaac ttattaaaat aatatttatc tacatttata tatgttatag cttatgtttt
    13381 aattaattta taatttttaa attcaccaat agaaatctta ttattaaata ataatttttc
    13441 tattttagta agaatacgat gtcttttcga tggtttttta tacaggttat ctgctttttt
    13501 agaataattt aattgatcct gccaattaaa ttttttgatc cattcattca ttactgctgg
    13561 atgggtttct ttaaaaatag gcaaaactcc taaaggtcca taagaaaacg tttctatatg
    13621 ttgattttca tcttttgtta aattattaac acctttatga atagtatcta aagcaatttt
    13681 ctttttttgc attaattgcg gtggcctcac ccaaccataa tgatatattt tggcatcaac
    13741 tttagctact tttagtttat aagtatcttt ttgttgtcta tagtgtaatc catcaaaatt
    13801 tggaatcctt ctaaaagatt gtgcgctttc ccaagaatga atatctggat catttctaat
    13861 gatacgaatc tcttttttat accaaccatg cccattttga taatgccaat aatctccata
    13921 aaaatgaata taatcgaaaa gcaatccttc tatttcgtca ttatcaatta actcctcaca
    13981 tctttttatg atattttcta aatatttctc gtgtactacc tcatcagctt gtaaataaaa
    14041 taaccaatca ccactacaag cacttttagc tatatctgtc tgatgagcat tttcggtgcc
    14101 attaggatat ttatctaaat cccatttcga ttgaattatt ttaatttttg ggctattaat
    14161 actacttaca acttcgagag tcttgtcgtc atcatcacag tcccctatat ttataataaa
    14221 ctcatctact ataggcaaaa ttgataatat agattgcttg ataggataat acaatttatc
    14281 tgcatttctg caaaaagtaa aaccactgat tcgcattgta ttttttttga ttcaaaatta
    14341 aataaaaata ttaattatta taaaatttat taataaatct tgattaatat ttatcgaaat
    14401 acacagccat ccgaacaatt ttaataatag ctaaaaaatg gtcttgtaag tcagtattca
    14461 tgtggaaata ttttaaaaaa tcaagaaaat atgcaaaaac tgccatgtat tcataatatt
    14521 tcaattataa caaaactgta aaaacatgtc tacctgatta tgaaattgat ttattaataa
    14581 taatatagac agatagcaat aaggtaataa atctaaataa ataaactatg ttttttttaa
    14641 aaaaaatcat ataattttat gtaattttgc tataaaatta ttttatatgg ccttaataaa
    14701 aagcgtcaaa ggtttatctc ctaagtgggg aagtgagtgc tggatagccg aaaatgctac
    14761 aatagtgggc gaagtagtaa tgggtaataa atgtacagta tggtttaatg cagtaatacg
    14821 tggcgatgta cattatatac atattggcaa taatgtaaat attcaagatc aggtagtaat
    14881 acattgtact tatcaaactt cgcctgtgga gattggtaat aatgtatcaa taggtcatgg
    14941 tgccatagta catggttgca aaattggtaa taatgtattg ataggaatgg gagctataat
    15001 aatggatgac gtgataatag aagataactg cataatagct gcaggagcta ttattactaa
    15061 aaatactcac gtgtcttcgt atagtgtaat ggcaggtatc ccagctaaaa aaattaagaa
    15121 tcttacaaaa gaattgtttc aaaaagaagt agtagatata gctaatcatt atcctattta
    15181 tgctaaatgg tatgatatat agatttttta gaataattca gttaaaaatt tatcggcttt
    15241 caatatcagt atgtttagca gaacgagagt aaaaaggatt gggtattgaa aattaatatt
    15301 tgttatactt gaagcggttc aactatattt cgtactcatc agccgccaat actcatctca
    15361 cgcccatctg tcgcctaatc agttagctct gtaggtattt ggtagctacg tcaataaatc
    15421 accacaaccg aaagatattt ctatttcttt aaatgatagt ataaaatacg taaagtataa
    15481 caaacataaa tttcatgaaa tcccaccacc attactacac cagctcacat atcgttattt
    15541 ttattttgtt aaaaacttta aatcttataa aaaacaattc ctatgattaa tttataaaat
    15601 cactattatt tgctttaact ttgatagtat ttttattaat aataaaaaat atttttcaat
    15661 atttttaaaa tgtttagtta cgaatggtaa aatatctaat taaagattat cattttattt
    15721 ctaaaaaaaa tcttaatttt aacaaaaaaa tatttacaaa attttgatat gaaaaatcta
    15781 aaaatttata tatctacata tgtgtgctta ttatttatta ctctgtgtaa tggccaaagt
    15841 gcttatagtt ttttagattt tggtaataat gctagaagca atgctcttgg cagtatgcta
    15901 tattcggttt atgaatctga tgttttgagt tattaccata atccgggttt cataaatgat
    15961 agtgatgcgt acagtattgg tttcaattat caaacatatg ctgcacatat ttctcaatat
    16021 actctaaatt ttaatccata tattcgtaat tttgggacct tagctttcac tattaaatat
    16081 ttagattatg gtaatttcaa aggatatgac tggacaggtg atcccaccaa tgatttttca
    16141 gtatatagta cagtttttgc agtaggatat ggcaagcaat tatttgataa attcaatata
    16201 ggaatcaatc taaaaggtgc ttatatgcga gccgaaaaat atggctttgc tttagcttca
    16261 gatgtagcat tgaattatta tgatacaata aataaattat cactttcttt atttttaaga
    16321 aatatcggac atgaggtaag tcaatctttt ggtgatgaaa aaaatagttc tttgcctttt
    16381 aatttggcat ttgcaatgtc taaaagattt aagaatgcac ctttcagatt tcatctttat
    16441 tttcacgaca ttcataattg gaaattacgt tttacagata ctacttcctt acaaatagat
    16501 ccatttacta atgaatatat agaacctaaa aaatttgcta aaattactga tgaatttatg
    16561 cgtcattttg cctttggtat agaaataata ccaacaaaat ttttgagttt gatgatgggg
    16621 tataattatc aaaacagaca agaaatgaca atagaaagtc gcaaaggttt gattggtttt
    16681 tcttttggag taggtcttta tactcgtaga ttttctttta tttattctta taacatttct
    16741 catttagctt atggacctaa taatttttca ttaattatac ctctcaaaat atatttttag
    16801 ttaataatat aaaaaatcaa tttatggata gaaatcccaa ggtaaaaacc ttaaagaatt
    16861 tagcaagtta tttagaaaaa aaagctaatc taaaacaaga tgtatatgat aatactaaaa
    16921 aagcttttga tttgattaaa caagaagcca tgtttttatt aaaagatata agggggaata
    16981 tgctaaataa aaaaagagta atcccattag aaattataga acgtacagat tttgaatttg
    17041 aaatcaaatt tgctggtgat attttagctt tttttatgca tactaatgct tttatgttac
    17101 caccttatca tcctgcaact cagaatagat atgctcaaga aaataaagaa aatacttttg
    17161 tgggaataat taatattttt aattttttag cagattcttt taaatataat aggtataatg
    17221 atgtggggtt tttgataggc agaatttatg taaacaaaga caatcatttt tatatagatg
    17281 gtaggtctga aatacaaaat atacctattc aattcgaaac tcagaaaatt tctgcaaaag
    17341 aaatgagaaa tgttttaatc aattgcatcc tttctactat agattttgac ttgatagcac
    17401 ccccattaga atattatgaa gctataccag taaaaacttt catagatgca atcgaaagct
    17461 atcccattaa aacaagcaaa ttagttggtt ttaaagtaaa tagttttcaa aataaatgat
    17521 ttgtgtgcct agtaaatatc aaacgtttta tttatttttt ataaaattaa aaactgttct
    17581 accaatatca gctgggctat cgataatatt tattccacaa gatctaaggt aatctttttt
    17641 ggcttgtgct gtatcatctt tccccccaat aatggcgccg gcgtgtccca tagtacgacc
    17701 tttaggtgca gttactccag aaataaaagc aaaaactggt tttactttat tatcttttac
    17761 ataatctgca acttcatttt cggtgccacc tcctatttcc cctattatca ctattgtatc
    17821 agtatcatca tcattcataa atagttccac tatttctttc attgaactac ctaaaatagg
    17881 atcgccacct atgcctacca tagaagattg tcccaatcca agtctagtaa gttgatacac
    17941 tacttcgtaa ctgagagtac cgctacgact agctatacca atattccctt tcttgtgtat
    18001 aaaaccaggc attataccca ctttagcttc acctggagtg atcacaccgg gacagtttgg
    18061 tcctatcaaa cgagtagaag tattcttaag aaacgctttt acttttatca tatcttgaac
    18121 aggtatacgt tcggtaatgc aaattatcaa ttcaattcct gctgaggccg cttccacgat
    18181 tgcatcagca gcattggacg ctggaacaaa aattattgaa acattagcac ctgtctccat
    18241 tatagcttta tcgacagtat taaatatggg tatatcgtta aattttgtcc cgcctttttt
    18301 tggtgttact cctgctacta cttttgtacc atattctatc atttgctgag tgtgaaaact
    18361 cccctcttta cctgtaatac cttgaactaa tactctcgat ttataattca ctaaaatact
    18421 cattaatgtc cgtttaatta aaattaaatc aaaattaata atattttaca tattaaaata
    18481 ttattaattt tgaaaatcta gaaacaaatc attgttttac ttgaaatagt ttatctatac
    18541 tttgtattca ccagccgcca atactcatcc cacgcccatc tatccgaaga tgcaaggttt
    18601 aaaagccgac caatgtcaga ttctatctca cttgttaagt atttatgtgt gattacaaac
    18661 cacatcagat agctctgcag gtatttagta gccactcctc taaatcgtcg caaccaaacc
    18721 ttgaaatcat ttacatgctt atcgactacc tgtacactgt aaacaccacg tctaattatt
    18781 taattatgct ataaactacg taaagtaaaa caaaggatat ttttaagtag ctccgttcac
    18841 aaatagcgtt tacgaagcgt aatttttaca aagctaaatt tcttatccat ttttatttga
    18901 ataaagacta aaaaaaagtt ctataacgtt ttgtatag
//
LOCUS       DAOPRN010000037        18640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_2518_length_18640_cov_112.166371, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000037 DAOPRN010000000
VERSION     DAOPRN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2518_length_18640_cov_112.166371"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..959)
                     /locus_tag="PKI65_05405"
     CDS             complement(183..959)
                     /locus_tag="PKI65_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77911.1"
                     /translation="MIKGVKYIPLRAFKINFGLIRCMYKQVIGHLKKTMTRVKLIFIL
                     TVLALTNVLGQDNKVICDCPKTQFAGTRADTTFYLSNGKTIVLCGYKNPESKPTTFSE
                     FILAVCGQDTIIDFWGAVQTCRLNVNKDTLFVSELKNLPTGKNFKYQETVWTTEKIFF
                     NGQKVVRKLFVNRQIKKYNQDEIQTVLKAYETAKSGLDECKMEIANRLFIATISGDKK
                     ARQYFKEFKNKFGTLNGAFAEEYSDLIAMLDLWDKKNNVP"
     gene            1205..3061
                     /locus_tag="PKI65_05410"
     CDS             1205..3061
                     /locus_tag="PKI65_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOB77912.1"
                     /translation="MYKSIILIFICISNILMAQIYNPIIDSIAMRDGKKIAADIYLID
                     SINPRPTILIQTPYNRIYYRLNLPLVGHNIEDYGYNFVITDWRGFYGSSLAYVAGYDR
                     GLDGYDLVEWIAQQPWSNGKIGTYGASALGKIQFKTAKEQPPHLVCCVPLVANPQMTY
                     LEYFPGGVYRTEYVQQADNLGFGYSPWLLSNPFYNFLWQFVENSTFYPSSINVPFFMI
                     GGWYDHAIDQKLNFFNALMQQSPVSSQHKLLMGPWVHGGHGISQVGSSQQGELFFYNA
                     SNWNDSLSLRFFDFYLKNVNNGWNNEPVMQYYQIGDNQWQQATSLSAINVDTLQMFLN
                     SDNYLTTIPPDVQNQNEYFIYNPADPSPTYGGSTLRQDLLQGPYDQSQVVESRSDLLI
                     FTSSTLNEPVRIFGKIKITLFVSSNCKDTDFAIRFCDVYPDNRSILINDGIKRMRFRN
                     GYQTSDTASMLDGVIYKIDIFLPHTAYTFLPGHKIRIDISSSNYPKYDNNLNNGKQMY
                     TAGDTIIATNVVYYETDHKSSISLPILHSNNICDFSNSERFIIYPNPTNSSFTISNFN
                     QIKFIEIYDIMGKKYQFNEQVNFNVNNLKNGLYLIKITDVNDEIIWKKLIKN"
     gene            3204..4631
                     /gene="sufB"
                     /locus_tag="PKI65_05415"
     CDS             3204..4631
                     /gene="sufB"
                     /locus_tag="PKI65_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOB77913.1"
                     /translation="MQDDNFDWIKEQEYEYGFETPIEVEVFPKGINEDIIRRLSSIKG
                     EPDFMLEFRLKAFEYWKNMIPPTWGHLKFDPIDFQDISYYAAPKKLKSNEIDPELLET
                     FEKLGVPLEEREILAGVAVDAIFDSVSVKTTFREELDKLGIIFCPISEAIRNHPELIK
                     KYLGTVVSPYDNFYAALNSAVFSDGSFVYIPKGVKCPVELSSYFRINTQNIGQFERTL
                     IIADEESYVSYLEGCTAPMRDENQLHAAVVELIALDNAEIKYSTVQNWYPGDKEGKGG
                     IYNMVTKRGICKGKNSKISWTQVETGSAITWKYPSCILSGDNSIGEFYSVAVTNNYQQ
                     ADTGTKMIHIGKNTRSTIISKGISAGYSQNSYRGLVKITKNALNSRNYTQCDSLLLTD
                     HCGAHTWPYIDVANDESIVEHEATTSKISEDQLFYCNQRGISTEEAIRLIVNGYTREV
                     ISKLPMEFAVEAQKLLSLSLENGIG"
     gene            4647..5387
                     /gene="sufC"
                     /locus_tag="PKI65_05420"
     CDS             4647..5387
                     /gene="sufC"
                     /locus_tag="PKI65_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOB77914.1"
                     /translation="MKLLEVKNLNVFVENKHILKDFSLVVNNGEVHAIMGPNGTGKST
                     LAHVIAGKPGYIIEGEILYKGKNLLDMSIEERAREGIFLSMQYPVEIPGVPMLTFLRT
                     AVNEIRKSKGLESLSPAEFMKYIESKKSIIEIPADLLKRAVNVGFSGGEKKRNEIFQM
                     AILDPILAILDETDSGLDIDAMKIVANGVNNMRSPERAFIIITHYYRLLEYIEPDVVH
                     IMIDGRIVKTGDKYLATEVEETGYDIYR"
     gene            5400..6779
                     /gene="sufD"
                     /locus_tag="PKI65_05425"
     CDS             5400..6779
                     /gene="sufD"
                     /locus_tag="PKI65_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOB77915.1"
                     /translation="MKSKLLEQIKEIAPKLLSIEDLWTNRDQHYHTFLSLGFPDDKVE
                     QWRQTPINDQLNKPLYYVTDNSPKQIERLDDYFSCPIENFEKYLFLIHNGAPLYSDQV
                     IFKDPNTGMIAGSLRHAFLLFPDIFNKYFDNIADPAYNGFVALNMSLASNGFFLYVPS
                     GVKVKLPVQLINYSKGDDNPFIQSRNLIILDDNAEITFLQCDDTHMSVDNSFYNILTE
                     FYIGKGATLYHYKLQNKSNNSSLINTMFFTLNESSNLHSLSLTYNGGFIRNETIINMD
                     APEANAKIYGLGLIDRQQIIENLAFINHNAKNCTSYQTFKNIIDEEGRGLFNGHIKVM
                     PGAQKTSAYQLTRNILLTDEARIFVKPFLEIYADDVKCSHGSSIGKLDDEALYYLMQR
                     GICEKNAKLLLMYAFTNDIVAEIHISDLKKQTIGMINRRLKGELSACDQCILGCTKFE
                     IPELDFSDF"
     gene            6896..7915
                     /locus_tag="PKI65_05430"
     CDS             6896..7915
                     /locus_tag="PKI65_05430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOB77916.1"
                     /translation="MFNKSNKYLLIQTASIGDVVLISPVIESIHNYEPQSDICILTNT
                     RGAELFHKHPFIKDVFVWNKKKNKYKNLLKIIKDLRKYKFKAVINFQRHLSTALIMGS
                     MHSDKKITFKQSTFSWLFANERFEHEWNKKHEVDRNLTLIRNIVPEEYQVRKPKLYIP
                     DDIHVDLPPSPYVCLSPYSLWYTKQMVVWRWIDLCENLIKLGYNVAILGGNEDVKKNN
                     LFFKKFKINDHLYDFVGKLSLLETLYVISNAIITYSNDSAITHLASSVNAPIATIFCS
                     TVPSFGFTPLSDNSFIIEPNIEILCRPCGMHGHQKCPKDLFICGNSFIIEQLLEPLKK
                     LSLHE"
     gene            7908..8489
                     /locus_tag="PKI65_05435"
     CDS             7908..8489
                     /locus_tag="PKI65_05435"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOB77917.1"
                     /translation="MNNPVINSNEIEIIVNLLKQGRVILCPTDTIWGLSCNALDEQAI
                     KKILKIKHRSINKGLIVLVSSWEMLKNYIPDIPQLAYDLENKSPRPITIIYPNPINLP
                     SMLIDKENTVAIRIVRNDFCKEIINSLGLPIVSTSANISGKPTPINFNDIDPEIKSMV
                     DYIVSAKYDYSQTNKPSRLIKIINDNEYIVIRE"
     gene            complement(8509..9210)
                     /gene="pyrH"
                     /locus_tag="PKI65_05440"
     CDS             complement(8509..9210)
                     /gene="pyrH"
                     /locus_tag="PKI65_05440"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOB77918.1"
                     /translation="MYKRILLKLSGEALIDQDGNSIGINQLEHYCSEISDAYKSGVQI
                     AIVIGGGNIFRGIQGAKRGFSRIQGDYMGMMATIINSLALEEMLLEYNIPSTILGAIE
                     IDNICKKFYFKKAIDLLENNKVIILSGGTGNPLFTTDSAAALRAIEINADILLKGTKV
                     DGVYTEDPFKNPNAQKYEYLSLDEAFEKKLKILDLTAYTLCMENNLPIIVYDATKKGN
                     LKKILEGEKIGTLIK"
     gene            9553..11574
                     /locus_tag="PKI65_05445"
     CDS             9553..11574
                     /locus_tag="PKI65_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOB77919.1"
                     /translation="MKTKVIFTTIFLVFIFNFSFAQKDIEIIEEIKKNKKYRNDYELA
                     QYYINNKMYNDAFNLLKKWETVYPDNSNLMYNIGICIYNSVDKMNAQQYFSKAAQNIA
                     KDHENIYTSINAPLDVYVYLANIYHLKYEFDKAIDYYDDFLINAYNYPINYIDSVKWL
                     KQKSYTAISYVKNPVTVKVANLGDSINTKYPEYSPVFNFVDSILYFTSRREGSTGGKK
                     TEDGKYYEDIYYSQLYKNQDEYLWTNAKQLAGELNTTGHEATVSLSPNGKMLLVYRDD
                     KGDGNIYYSEFIDGKWSALERFPTINSQYWETHACLSPDMKTIYFVSDRPGGYGGRDI
                     WKSERGEHGWLAPENLGPAINSKYNEDAPFILSDGVTLYFASEGFENMGGFDIFSASL
                     TEDGFWTTPENLGYPINTPGDDIFYYPSPDEKYAFYSSADVHGYGDQDIYLMTIEKPK
                     IREIKLIGRTIDALSFLPVKSDISIKNIDNNEIVSELITNNDGHFEINLLPAYQYAII
                     ASANGYNDNMKAITLIENDPRSVILIDIFLEKPVIAHKEESLIKEEQIKVGTSIVLRN
                     IYFDFDKATLRPQSIEELDKLYDFLINNPTAIVEVSAHTDKIGSYEYNIKLSKKRAQA
                     VVDYLIAKGIEPNRLIAKGYGYTKPIATNLTEAGRQLNRRVEFKIVGLE"
     gene            12142..13107
                     /locus_tag="PKI65_05450"
     CDS             12142..13107
                     /locus_tag="PKI65_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOB77920.1"
                     /translation="MLKEKFHIKQKKDTRSGFGDELYELGTNNPKVVALTADLTGSLK
                     MDKFAKSFPDRFIQVGIAEANMMGIAAGLANAGFIPFTGTFAVFSTGRVYDQVRQSIA
                     YSYKNVKICASHAGISLGEDGASHQMMEDIALMRALPNIIVINPADYTQTRQAVRAIA
                     KYLGPVYLRFGRPAVPDFIPDDMPFEIGKAITLKTGKDVTIFATGHLVWEAVEACDEL
                     EKMGIDAELVEIHTIKPIDENAIINSIKKTKCAVTAEEHVLHGGLGDAVAQVSVKNYS
                     VPIEYVAMDCVFGESGTPNELMVKYGLKSTNIVNAAINSIKRKNY"
     gene            13111..13662
                     /locus_tag="PKI65_05455"
     CDS             13111..13662
                     /locus_tag="PKI65_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOB77921.1"
                     /translation="MNEPSDDYIISLFSESDTRSEGFKLLLKKYKEKVYWHVRTIVVS
                     HDDANDVVQNVFIKIWENIDNFRGDSKLYTWIYRIATNEAINFINKKRSNLFISIDEL
                     QENFLDNLEADPYFKGSEIERKLIKAINTLPPKQKLVFYLRYYTQMPYKDMAEVLNTS
                     INTLKSSYHQAKKKIKKFFLSID"
     gene            13708..14919
                     /locus_tag="PKI65_05460"
     CDS             13708..14919
                     /locus_tag="PKI65_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB77922.1"
                     /translation="MKFENKENEIIKNAFQGFEVPENYFDSLEKNILNKTINKRKIVN
                     KKILSYSLISLSLIAISLIVFFKSSTNNKIDFQNLKSNYHLHILYNDPNLFLLDKKNN
                     ILYINIDSSEHSILQAIEDIDIPEKDKYNLLMSYKLLKYSLSEYRNNKLEILEYEQID
                     EANNKKYKYFDTINSNKLFNIPREICSEKPIVLEPSIANKDAYQYKWSNGETSDKIYI
                     TESGTYTLTITPIKSEDPKVLLATTKVLIIPPPKDLSPKTILGCVGSEVELDLNIDTS
                     KYNIEWINLRKKSKSIKVTKPGTYIAKITGCQTYLDSFIVSFTHCDIKIPNAITPNGD
                     GVNDVFYITNIENYPNTQLFIYSNDGNLIYHNKNYQNDWDASNTPPGTYFYKIIFPDK
                     IIQNGTLLIIK"
     gene            complement(15108..15308)
                     /locus_tag="PKI65_05465"
     CDS             complement(15108..15308)
                     /locus_tag="PKI65_05465"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOB77923.1"
                     /translation="MKKCNIELNRLKCNCTYEPCSRKGICCECLRYHLESEELPACFF
                     TPKIEKTYDRSIKKFIDLYNNK"
     gene            complement(15320..16003)
                     /gene="trmD"
                     /locus_tag="PKI65_05470"
     CDS             complement(15320..16003)
                     /gene="trmD"
                     /locus_tag="PKI65_05470"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOB77924.1"
                     /translation="MRIDILTVFPEMFIGPFDYSIIKRAKDKGLIDIYIHNLRDFSND
                     RHRRVDDYPYGGEAGMVLKVEPIANAIEFLNKQRSYDEIIFLTPDGTPFTQSIANKLS
                     LMNNIILLCGHYKGIDERVRELFITMELSIGDYVLSGGELPAMVVVDAITRLLPGVLG
                     DEQSALSDSFQDGLISPPVYTRPANFRGHKVPDILRSGDHNAIEEWKLQQSIKRTQIR
                     ENKKNNNEL"
     gene            complement(16009..16626)
                     /locus_tag="PKI65_05475"
     CDS             complement(16009..16626)
                     /locus_tag="PKI65_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOB77925.1"
                     /translation="MLHIAVSGNIGSGKTTLTELLAKQLNLNAIYENIDNNPYIISFY
                     NDMKRWSFNLQIYFLCSRFKQTMEIQKNQLRVVQDRTIYEDAHIFALNLFEMGLMEKR
                     DFDTYKDLFDLMISFLRPPDLLIYLQADVSTLVRQIQIRGREYETSIRLDYLQSLNEK
                     YENWINTYDLGKKLIINVDGKDFLHNPEDLGEVINLINANLFGLF"
     gene            16844..18310
                     /locus_tag="PKI65_05480"
     CDS             16844..18310
                     /locus_tag="PKI65_05480"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOB77926.1"
                     /translation="MNFSTYLLVGIIIAFIVLIASIFKRLNVPLILIALAIGIFFGSD
                     VTGLIYFDDAHLTKQITDIALMFILFAGGFETKLKDLKTVMTPALLLSSIGVILTGLI
                     SALFFSLIFKWDFIQSLLLCAIISSTDASAIFSILRKSDVDLEATSLTKLESATNDPM
                     AIIFTTFLIQLVSGVNFSTFSTVIIFLWLFIGGIVIGILIGFVGKFIFKRLKDLDAGY
                     YHLLILGLIFLSFGFAELCRANGLMAVFFTGLILGNTKLPYNSRIASFTDTLSFITNV
                     IIYVLLGLLAFPSKFPDLWVYGLVLFLILTLVARPITVFLLTSFTKLSTKKKVFVSWS
                     GVRGTVPIVLATYPIVAGLDPQHQIFNIVFIAVILSIVIQGGSMNKVSTWLKLATKRK
                     NTVQNLMELVTVHDTDYDLIEIFIDDQKYIGECQIADLKLPSNSTITMINRNNQVIAP
                     SGQTIIMPGDTLFVLVKKENIEIVIDRILNKFNKINEI"
BASE COUNT         6728 a         2543 c         2805 g         6564 t
ORIGIN      
        1 tttgttttat taacttataa agttataaaa actaaaaata tttttaattt tataatgcac
       61 cataacggcc agcggtatga aaagttgccg attgcgaacg ttttttgtca agttacataa
      121 aagttgaagt gggctacaac ccttgaataa cctactgtcc cggcaatttt ttataccgtg
      181 tgttagggca cgttattttt cttgtcccat aaatctaaca tagctatcaa atcgctatat
      241 tcttctgcaa atgctccgtt aagcgtccca aacttatttt taaactcttt aaaatattgt
      301 cttgcttttt tgtcacccga aattgtagca ataaatagtc tgtttgcaat ttccattttg
      361 cattcgtcaa gtccagactt tgctgtttcg tatgctttta acacagtttg tatttcgtct
      421 tgattgtatt ttttgatttg tctgttaacg aaaagttttc taacaacctt ctgtccattg
      481 aagaatattt tttctgtggt ccagacggtt tcttgatatt taaaattctt tcctgttggt
      541 aaatttttaa gttcgcttac aaacaatgtg tctttattaa cattcaatcg gcaggtttgc
      601 actgcacccc aaaaatcaat aattgtgtct tgtccgcaaa cagcaagaat gaactcagaa
      661 aaagttgtcg gtttagactc aggattttta taaccacata aaacaattgt tttaccattg
      721 gaaaggtaaa aagtcgtatc agctcttgtt cctgcaaact gtgtttttgg acagtcacaa
      781 ataactttat tatcctgtcc taaaacattt gttaaagcca atactgtcaa tatgaaaatc
      841 aattttactc gtgtcattgt cttttttaaa tgtcctataa cttgtttata catacacctt
      901 ataaggccaa agttgatctt aaatgctctt aatggtatgt attttacacc ttttatcata
      961 aaaaaattac atgaattcta aaaattcatt tttccacttt tgatccaaaa aaatcgttga
     1021 atctattaaa tattttttca aaaacagtac aaagataata taaaattttc aatattgtat
     1081 caaaataatt tatttttttt gcaaattttt tctatttatc tatatatcaa ctacttatat
     1141 ttacacacta atattttaaa aaaatattaa ttttaaaaat taatatccaa aatttttaaa
     1201 aattatgtat aagtcaataa ttttaatttt tatttgtata tcaaatattt tgatggcaca
     1261 aatatataat cccataatag actctatagc tatgcgtgat ggcaaaaaaa tagcagcaga
     1321 tatatacctt atagattcta taaatccacg accaacgata ttaatccaaa ctccatataa
     1381 taggatatat tatcgactaa atcttccatt agtaggtcat aacatagaag attatggata
     1441 taattttgta attacagatt ggagaggttt ctatggtagc tcactagctt atgtagctgg
     1501 ttatgacaga ggattagatg gttatgatct tgtagaatgg atagcacagc aaccttggag
     1561 taatggcaaa ataggtactt atggcgcttc agctcttggc aaaatacaat tcaaaactgc
     1621 taaagagcaa cctccacatc tagtatgttg tgtgccttta gtagcaaatc cacaaatgac
     1681 atatttagaa tattttcctg gtggagtata taggactgaa tatgtgcagc aggcagataa
     1741 tttaggattt ggttattctc cttggctatt gagcaatcct ttttataatt ttctatggca
     1801 atttgtagaa aatagtactt tctatccttc ttctattaat gttccttttt ttatgattgg
     1861 tggatggtac gatcatgcaa tagatcaaaa gcttaatttt tttaatgctc taatgcagca
     1921 atcaccagta tcttctcaac ataagctatt aatgggacca tgggtacacg ggggacatgg
     1981 aatatcgcag gtaggttcta gccaacaagg agaattattt ttctacaatg caagcaattg
     2041 gaatgattct ctttctttac gtttttttga tttctacctt aaaaatgtta ataatggatg
     2101 gaataatgaa ccggtaatgc aatattacca aataggcgat aaccaatggc aacaagcaac
     2161 tagtttgtct gctatcaatg ttgatacatt acaaatgttt ttgaatagcg ataattatct
     2221 cacaactatt cctccagatg tgcaaaatca aaatgaatat tttatttaca atccagcaga
     2281 tccatcacct acatatggtg gttctacatt acgacaagat ttattgcaag gcccttatga
     2341 tcaatcacaa gtagtagaat ctagaagtga tttactcatc tttacttctt ctacacttaa
     2401 tgaacctgtt aggatttttg gtaaaataaa aattacttta ttcgttagta gtaattgtaa
     2461 agatacagat tttgccatta gattctgtga tgtttatcca gacaatagat ctatacttat
     2521 aaatgatgga attaaaagga tgcgtttcag aaatggttac caaacaagtg atacagcttc
     2581 tatgctagat ggtgtcatat ataaaattga tattttttta cctcataccg catatacatt
     2641 tctcccagga cataaaatca gaatagacat ttcatcatct aattatccta aatatgataa
     2701 taaccttaat aatggtaaac aaatgtatac cgcaggagat actataatag ctactaatgt
     2761 tgtatattac gaaacagatc ataaatctag tatttcatta ccgatattac attcaaataa
     2821 tatttgtgat ttctcaaatt cagaaagatt cattatttat cctaatccta ctaatagttc
     2881 atttacaatt tcaaatttta atcaaattaa atttatagaa atttacgaca taatgggtaa
     2941 aaaatatcag tttaatgaac aagtaaattt taatgttaat aatctaaaaa atggacttta
     3001 cctaataaaa atcactgatg tcaatgatga aattatttgg aaaaaattga ttaaaaatta
     3061 atttttttgt tttacgtttt tagatctcag caaggtatta gaattgtatt ttttcgatat
     3121 aattaatcag tatgatactt gtcatttaat ttatcagttt taggttaatt ttattaattt
     3181 tgcaaaaata aaattataaa tacatgcaag atgataattt cgactggata aaagagcaag
     3241 aatatgaata tggatttgaa acgcctatag aagtcgaagt ttttcccaaa ggtataaatg
     3301 aagatataat acgtaggttg agctctataa agggtgagcc agattttatg ttagaattcc
     3361 gattaaaagc tttcgaatac tggaaaaata tgataccgcc cacatgggga catttgaaat
     3421 ttgatcctat agactttcaa gatataagtt attatgcagc tcccaaaaag ctcaaaagta
     3481 atgagataga tcccgaatta ttagaaacat tcgaaaagct cggtgtacct ttagaagaac
     3541 gagaaatatt agcaggtgta gctgtagatg ctatctttga tagtgtatcc gtgaaaacta
     3601 cttttcgtga ggagctagat aaattaggta ttattttttg tcctataagc gaagctatta
     3661 gaaatcaccc cgaactgata aaaaaatatt taggtacagt ggtctctcca tatgataatt
     3721 tttatgcagc tctcaattct gcagttttta gcgatggatc ttttgtttac atcccaaaag
     3781 gtgtgaaatg tcctgtggag cttagttcat attttcgtat taatactcaa aatattggtc
     3841 agtttgagcg aactcttatt atagctgatg aggagagcta tgtcagttat ctagaaggtt
     3901 gcaccgcccc tatgagagat gaaaatcaat tgcatgctgc tgtagtagaa ttaatagctt
     3961 tagataatgc agaaatcaaa tacagtacag tacaaaattg gtatcctggt gacaaagaag
     4021 gcaaaggtgg catttataat atggtgacca aacgcggtat ctgcaaaggt aaaaatagta
     4081 aaatttcgtg gacacaagta gaaactggct ctgctattac atggaaatat cctagttgca
     4141 tcttgtcagg tgataattct attggtgagt tttatagtgt ggctgttact aataattatc
     4201 aacaagcaga tactggcact aaaatgatac atattggcaa aaatacccgt agtaccatca
     4261 tttcaaaagg tatttcagct ggttatagtc aaaatagcta ccgtggttta gtgaaaataa
     4321 ctaaaaatgc tctgaattct agaaattata ctcagtgcga ttcattactt ctgacagatc
     4381 attgtggagc tcatacctgg ccatacattg atgtagctaa tgatgagtcc attgtagagc
     4441 acgaagctac tacatctaaa atctctgagg atcaattatt ttattgcaac caacgtggca
     4501 ttagcaccga ggaagctata agattgatag ttaatggtta tactcgtgag gtaattagta
     4561 agctgcctat ggagtttgcc gtagaagcac aaaaactttt atctcttagt ttagaaaatg
     4621 gtataggtta aaaaaaaata gtaataatga aattattaga agtaaaaaat ttgaatgttt
     4681 tcgtagaaaa caaacatata ttaaaagatt tctctctagt agtaaataat ggcgaggtac
     4741 acgctatcat gggacctaat ggtactggca aaagcactct agcacacgtg atagctggta
     4801 aacctggata tataatagaa ggtgaaatat tgtataaagg aaaaaatctc ttagacatgt
     4861 ctatagaaga gagagctaga gaaggtatat ttctcagtat gcaatatcca gtagagatac
     4921 caggagtgcc tatgcttact tttctgagaa ctgctgtaaa tgaaattaga aaatctaaag
     4981 ggttagaatc tctgtctcct gccgaattta tgaaatatat agaaagtaaa aaatctataa
     5041 ttgaaatacc agcagattta ttaaaaagag ctgtgaatgt aggtttctct ggtggagaga
     5101 aaaaacggaa tgaaatcttt caaatggcaa tattagatcc tatacttgct atcctagatg
     5161 agacagactc aggattagat attgatgcta tgaaaatagt agctaatgga gtaaacaata
     5221 tgcgctcacc agagagagct tttattatta ttacgcatta ttatcgtttg ttggaatata
     5281 tcgaaccaga cgtagtgcat ataatgatag atggtcgtat agtaaaaaca ggagataaat
     5341 atttggctac agaagtagaa gaaactggat acgatattta tagataaaat tttagtaaaa
     5401 tgaaatcaaa actcttagaa caaataaaag aaatcgctcc taaattgttg agcatagaag
     5461 atttatggac taatagagat cagcactatc atacattttt gagtttaggt ttcccagatg
     5521 ataaagtaga acagtggcgt caaactccta taaatgatca attgaataag cctctatatt
     5581 atgttaccga taattcacct aaacaaattg aaagattaga tgattatttt tcttgtccta
     5641 tagaaaattt cgaaaaatat ttattcttga tacataatgg agcccctcta tatagcgatc
     5701 aagtaatctt taaagatcct aatacaggca tgatagctgg tagcctgaga catgctttct
     5761 tgttatttcc tgatattttt aataaatatt ttgataatat agcagatcca gcatataatg
     5821 gctttgttgc tctcaacatg tcattagcat cgaatggatt ttttctttat gtgccttctg
     5881 gcgttaaagt caaattacct gtacaattaa tcaattattc caagggggat gataatcctt
     5941 ttattcagtc tcgcaactta atcatcttag atgataatgc cgaaatcaca tttttgcaat
     6001 gcgacgatac tcatatgtca gtagataata gtttttataa tatcttgaca gaattttata
     6061 taggtaaagg tgctacatta tatcattaca aattgcaaaa caaaagtaat aactcatcgc
     6121 ttataaatac aatgtttttt actctaaatg agtcatctaa tttgcattca ctctcactta
     6181 catataacgg cggttttatt agaaacgaga ctataattaa tatggatgct cctgaggcta
     6241 atgcaaaaat ctatggctta gggttaatag atcgtcagca aataatagaa aatctcgctt
     6301 ttattaatca taatgcaaaa aattgtactt cataccaaac ttttaaaaat ataatagatg
     6361 aggagggtag gggactattt aatggacaca taaaagtaat gccaggagca caaaaaactt
     6421 ccgcctatca attaacgcga aatattcttt taactgacga agccaggatt tttgtgaaac
     6481 cttttttaga aatttatgct gatgatgtca aatgtagtca tggatctagt attggtaaac
     6541 tagatgatga ggctctttat tatctgatgc aacgtggtat ttgtgaaaaa aatgctaaat
     6601 tattactgat gtatgctttt actaatgata ttgtagcaga aatacatata tcagatctta
     6661 aaaaacaaac tattggaatg attaatcgta gattaaaagg tgaactctct gcttgtgatc
     6721 aatgtatact aggctgtact aaatttgaaa taccagaatt agattttagt gatttttgaa
     6781 ttatatttac aggtattagt ttagtaataa atgatgaata aaatatttta atgagtatct
     6841 aaattattat ctcactaaaa aatttctatt acctttgtca tataattttt tttttgtgtt
     6901 taataaatct aataaatatt tactcataca aactgctagc ataggagatg tggttttgat
     6961 atctccagtg atagagtcga ttcacaatta tgaacctcag tccgatattt gcatattgac
     7021 aaatactcga ggagctgagc tttttcataa acaccctttt atcaaagatg ttttcgtttg
     7081 gaataaaaag aaaaataaat ataaaaatct tttaaaaata ataaaagatt taagaaaata
     7141 taagtttaag gcagttataa attttcaaag acacttatct actgcattga taatgggtag
     7201 catgcatagt gataaaaaaa ttacattcaa acaaagcacc ttttcatggt tatttgctaa
     7261 tgaacgcttc gaacacgagt ggaataaaaa acatgaagta gatagaaatt tgacattgat
     7321 acgcaatata gttcctgaag agtatcaggt tagaaaacct aaattgtaca ttcctgatga
     7381 tattcatgta gatttgccac catcacctta tgtatgtcta tccccatatt cattgtggta
     7441 tactaaacaa atggtagtat ggcgttggat agatttgtgt gaaaatttaa taaaactagg
     7501 atataatgta gcaatattag gtggtaatga agatgttaaa aaaaataatt tattttttaa
     7561 aaaattcaaa attaatgatc atttatatga ttttgtaggt aaattatcat tattagaaac
     7621 tttatatgtg atatctaatg ctattattac atattctaat gatagtgcta ttacacattt
     7681 ggcatcatca gtaaatgcac ctattgctac tatattttgt tctacagtac cttcatttgg
     7741 cttcacacct cttagcgata atagttttat aatagagcca aatatagaaa tactctgtag
     7801 accttgtggt atgcatggcc accaaaaatg tcctaaagat ctgtttattt gtggaaatag
     7861 ttttataata gaacaattat tagaaccatt aaaaaaacta agtttacatg aataatccag
     7921 taataaatag taacgaaata gaaataatag tgaatttatt aaaacaaggc agggtaatat
     7981 tgtgccctac tgatactata tggggactta gctgcaatgc attagatgag caagctataa
     8041 aaaaaatttt gaagataaaa catagatcta taaataaggg attaatagtg ttagtctcat
     8101 cttgggaaat gttaaaaaat tatatacctg atatcccgca gttggcttat gatttagaaa
     8161 ataaaagccc tcgccccata actattatat atccaaatcc tattaatcta ccatctatgc
     8221 taattgataa agaaaatact gttgctatta gaatagtgcg taatgatttc tgtaaggaaa
     8281 ttattaattc tctcggttta cctatagtat ctacttcagc taatatttct ggtaaaccta
     8341 ctcctattaa ttttaatgat attgatcctg aaatcaaaag tatggtagat tatatcgtgt
     8401 ctgctaaata tgattattca cagacaaata aaccttctag attgattaaa attattaatg
     8461 ataatgaata tattgttatc agagagtaaa attaatattt tttttgaatt atttaatcag
     8521 agttccgatt ttttcgcctt ctaatatttt tttcaaatta ccttttttag tagcatcata
     8581 tactataata ggtaaattat tttccataca tagagtataa gctgttagat ctagaatttt
     8641 taattttttt tcgaaagcct catctaaact aagatattca tatttttggg cgttaggatt
     8701 tttgaatgga tcttctgtat aaacaccatc tactttggta cctttcaaaa gaatatcagc
     8761 attgatttcg atggctctta aggcagcagc agaatcagtt gtaaataatg gattgccagt
     8821 gccaccagac aaaataataa ctttgttgtt ttctaataaa tcaatagctt ttttgaaata
     8881 aaatttttta caaatattat ctatttctat cgcacctaat atggtagatg gaatattata
     8941 ttctaaaagc atctcttcga gagctaggct attaataata gtagccatca tacccatata
     9001 gtcaccttgt atacgagaaa aacctctctt tgctccctgt atgcctctga aaatattacc
     9061 acctcctatt actatagcta tttgtacccc cgatttatat gcatcagaga tttcagaaca
     9121 gtaatgctct aattgattta tgccaatact attcccatct tggtcgatga gtgcttcgcc
     9181 actaagtttt aaaagtattc ttttatacat atttttttgc taaaataaaa aattttttga
     9241 atttattcat ttattaataa aattgaaagt gaaatttaat tgaaattatt aatatttatt
     9301 tagcttacta aaacaattaa taaatggtgt ttaatttact agaaataaaa ttataaagtg
     9361 tagatttaca aatacaatga caataacaga cattgacagc aaaagatatg aaattttatt
     9421 agaaagcttc aatactaaat atctagccaa ggaaagatta gttggtttta aaactaaaat
     9481 agttaaatta ataagtaaat tatagagttg ataaattatt ttttatgtaa ttttgctaaa
     9541 aatatatata aaatgaaaac aaaagtaata tttacaacaa tttttctggt ttttattttc
     9601 aatttttctt ttgcacaaaa agacattgaa ataatagaag aaatcaaaaa aaataaaaaa
     9661 tatagaaatg attatgagct agctcaatac tacattaata ataaaatgta taatgatgca
     9721 tttaatttat taaaaaaatg ggagactgtc tatccagata atagtaatct gatgtataat
     9781 ataggtatat gtatttataa ttccgttgat aaaatgaatg cccaacagta ttttagcaaa
     9841 gctgctcaaa acattgcaaa ggatcatgaa aatatttata ctagtattaa tgccccatta
     9901 gatgtttatg tttatttagc taatatttat catttaaaat atgagtttga taaagctatt
     9961 gattattatg atgatttttt aataaatgcg tataattatc caattaatta cattgatagt
    10021 gttaaatggc taaagcaaaa atcatataca gctatttcgt atgtaaagaa tcctgttact
    10081 gtgaaagtag cgaatttagg agactcaata aatactaagt atccagaata ctcaccagtt
    10141 tttaattttg tcgattcaat tttatatttt acatctcgta gagaaggtag taccggtggt
    10201 aaaaaaacag aagatggtaa atattatgaa gatatttatt attcccaatt atataaaaat
    10261 caagatgaat atttatggac taatgctaaa caattagctg gtgaattgaa tacaacgggg
    10321 cacgaagcta cagtgagttt gagtcctaat ggcaaaatgt tattagtgta tcgcgatgat
    10381 aaaggagatg ggaatattta ttatagcgaa tttatagatg gtaaatggtc tgctctagag
    10441 agattcccta ctattaattc tcaatattgg gaaacccatg cttgtttatc accagatatg
    10501 aaaactatct atttcgttag tgatagacct ggtggttatg gtggtagaga tatatggaaa
    10561 agtgaacgtg gcgaacatgg gtggctagca ccagaaaacc tagggcccgc tattaatagt
    10621 aaatacaatg aagatgcccc atttattctg tcagatggtg ttactttgta tttcgcttct
    10681 gaaggtttcg aaaatatggg tggttttgat atcttttctg cttctttaac agaagatggc
    10741 ttctggacta ctccagaaaa tttgggctat ccaattaata ctcctggcga tgatattttt
    10801 tattatccta gccctgatga aaaatatgct ttctattcta gcgcagatgt ccatggatat
    10861 ggagatcaag atatttatct aatgacaata gaaaaaccta aaattagaga aataaagctt
    10921 ataggtagaa cgattgatgc attatctttt ttaccagtga aatcggatat ttctatcaaa
    10981 aatatagata ataatgaaat cgtttctgaa ttaataacaa ataatgatgg tcacttcgaa
    11041 ataaatttat taccagccta tcaatatgca atcatagcat ctgctaatgg ttacaatgat
    11101 aatatgaaag caataacatt gatcgaaaat gacccccgaa gtgtaatatt aatagatata
    11161 tttttagaaa aaccagttat agctcataaa gaagaatctc taataaaaga agaacagata
    11221 aaagtaggaa catctattgt acttagaaat atctattttg attttgataa agctacactc
    11281 agacctcaat ctatcgaaga actagataaa ttatatgatt tcttaattaa taatcctact
    11341 gctatcgtag aagtatcagc tcatacagat aaaataggta gctatgaata taatattaaa
    11401 ctatctaaaa agcgtgctca agcggtagta gactatttga tagctaaagg aatagagcct
    11461 aatcgtttaa tagctaaagg ctatggatat actaagccta tagctacaaa tctaacagaa
    11521 gcaggtagac agttaaatag acgtgtagaa ttcaaaattg ttggattaga ataattttta
    11581 ttttaccttt tgataatata ttagaactat caaaaaatta tggttctata acgtttattg
    11641 tggataaatt atttaggttg atttttttac cacaaagggc accaagttat tacacaaagt
    11701 ccacaaagtg ccatttatag tccttttaat ttcatccttc tctgtgttct ttgtgcattc
    11761 tttgagaact ttgtggttaa tatttaacta cactataatt aataaactaa tattaacaaa
    11821 ttttttataa aattactctt tttttaatct ttacccatac aaaacgttat agaaccgaaa
    11881 ttatatataa atttaatcaa ataataaata ctttaaacta tcaaattacg caaacataat
    11941 atttattttt gaatatgtgt attttgaatt cttgttttat aaaaatatat attcagattt
    12001 attattactt gtagatagtt tgttaatatg ttttaatata ttttataaaa attttataat
    12061 tttaacaatt aaagtgatta attatgtata tttgcatata ttttttgtaa aaaaaattta
    12121 aaaaatcaca tttatagagt tatgctaaaa gagaaatttc atattaaaca aaaaaaagac
    12181 actagaagcg gttttggtga tgaattgtat gaattaggaa ctaataatcc taaagttgta
    12241 gcacttactg ccgacctgac aggttcatta aaaatggata agtttgccaa gagttttcct
    12301 gatagattta ttcaggtagg tatagcagag gccaatatga tgggaatagc tgctgggtta
    12361 gctaatgctg gttttattcc tttcacggga acctttgctg tattttctac tggtagggtg
    12421 tatgatcaag tgagacagag tatagcttat agttataaaa atgtcaaaat ttgtgcgtca
    12481 catgctggca ttagtttggg tgaagatggt gctagtcatc aaatgatgga agatatagca
    12541 ttgatgcgtg cattacccaa tataattgtt ataaatccgg cagattacac tcaaacacgt
    12601 caagctgtta gagctattgc taaatattta ggcccagttt atttgcgttt tggtagacca
    12661 gcagttccag attttatacc tgatgatatg ccttttgaaa taggcaaagc tattactttg
    12721 aaaactggta aagatgtcac tatatttgct actggtcatc tagtatggga agcagtagaa
    12781 gcttgtgatg aattagaaaa aatgggtata gatgccgaat tagtagaaat acatactatc
    12841 aaacccatag atgaaaatgc tattattaat agtattaaaa agactaaatg tgctgttaca
    12901 gcagaagaac acgttttgca tggtggccta ggtgatgctg tggctcaagt atcagttaaa
    12961 aattattcgg tgcctatcga atatgtagct atggattgtg tgtttggaga aagtggaacc
    13021 cctaatgaat tgatggtaaa gtatggtctg aaatctacaa atatcgtaaa tgctgctata
    13081 aattcaataa aaagaaaaaa ttattaatag atgaatgaac cttcggatga ttatattatt
    13141 tcattgtttt ctgaatcaga tactagatct gaaggtttta aattattatt aaaaaaatat
    13201 aaagaaaaag tttattggca tgtacgtact atagtagttt ctcatgatga tgctaatgat
    13261 gtggtgcaaa atgtttttat aaaaatttgg gaaaatattg ataattttcg tggagactct
    13321 aaactctata catggatcta tcgaatagct actaatgagg ctataaattt tataaataaa
    13381 aaacgttcta atctttttat ttctattgat gaattgcaag aaaatttttt agataattta
    13441 gaagctgatc catattttaa aggtagtgaa atagaacgta agcttataaa agctattaac
    13501 actttgcctc ctaaacaaaa actcgtcttt tatttaagat attacaccca aatgccttat
    13561 aaagatatgg cagaagtatt aaatacttct ataaatactc ttaaatcttc gtatcaccaa
    13621 gcgaaaaaaa aaataaaaaa atttttttta tcgatagatt aaacttttta attaatttta
    13681 tgtctaatta ttaaaataaa aactaaaatg aagtttgaaa acaaagaaaa tgaaataatt
    13741 aaaaatgcct ttcagggatt tgaggttcct gaaaattatt ttgattcttt agaaaaaaat
    13801 atattgaata aaactattaa taagagaaaa atagttaata aaaaaatctt atcttatagt
    13861 ttgattagtt tatctcttat tgctatatca ttaattgtgt tttttaaatc ttcaacaaat
    13921 aataaaattg attttcaaaa tttaaaatct aattatcatt tacatatatt atataatgat
    13981 cctaatttat ttttgctaga caaaaaaaat aatattttat atataaatat agattcaagt
    14041 gaacattcca tattgcaagc tattgaagat atagatattc ctgaaaaaga taaatataat
    14101 cttttaatga gttataaatt attaaaatat agcttatcag aatacagaaa taataaatta
    14161 gaaatattag aatatgagca aatagatgaa gccaataata aaaaatataa atactttgat
    14221 acgattaatt ctaataaatt atttaatata cctagggaaa tatgctctga aaaaccgata
    14281 gttttagaac catctattgc taataaagat gcatatcaat ataaatggag taatggagaa
    14341 acaagtgata agatctatat aaccgaatcg ggtacttata cactaacaat cactccaata
    14401 aaatcagaag acccaaaggt attattagct acaactaaag ttttaattat accgccacct
    14461 aaagatttgt ctccaaaaac tatcttaggt tgcgtaggta gcgaagtcga gttagattta
    14521 aatatagata ctagtaaata taatattgag tggataaatt taagaaaaaa gtctaaatct
    14581 attaaagtca ctaaaccagg tacgtatata gctaaaatca caggatgtca aacatattta
    14641 gacagtttca tagtttcatt tacacattgt gatataaaaa ttcctaatgc tataacgcct
    14701 aatggtgatg gcgtaaatga tgttttttat attacaaata tcgaaaatta tcctaataca
    14761 caattattta tttattctaa tgatggtaat ttaatttatc ataataaaaa ttatcaaaat
    14821 gattgggatg ctagtaatac gcctccagga acttattttt ataaaataat atttccagat
    14881 aaaataattc aaaatggtac tctactaata ataaaataaa cgctctgctt tctacaccca
    14941 caacttacaa aaagatatgc ggtatacata ccgcatatct ttttgtataa atttagataa
    15001 aattaatata aatcaagcat aagcttgtat ggatgaaaaa aactatgact aatagaaata
    15061 aaatgtaaaa gagtagaagt ttttttttta aaaatttcaa tttaacatta tttgttattg
    15121 tataaatcaa taaacttttt gattgatcta tcataagttt tttcgatttt tggagtaaaa
    15181 aaacaagctg gtagctcttc actttctagg tgatatcgta aacattcaca acaaattcct
    15241 tttctagagc aaggctcata ggtgcaatta catttcaatc tgttaagttc tatgttacat
    15301 tttttcataa taaaattttt tataattcat tgttattttt tttattttct ctaatttgag
    15361 ttcttttaat agattgttgc aatttccatt cttcaatagc attatgatct ccggacctca
    15421 atatatctgg tactttgtga cctctaaagt ttgcagggcg cgtataaact ggcggggata
    15481 ttaatccatc ttgaaaagaa tctgataaag cagattgctc atcacctaaa acacctggta
    15541 acaatcttgt gatagcgtcc actactacca ttgctggcaa ttcaccacca ctaagtacat
    15601 aatctcctat tgacaattcc atcgttataa atagttctct tactctctca tctatacctt
    15661 tgtaatgtcc acatagcaaa attatattat tcattaaact taatttattt gcaatagatt
    15721 gtgtaaatgg agtgccatca ggtgttaaaa aaataatttc atcataagat ctttgcttat
    15781 ttaaaaattc tatagcgtta gctattggtt ctacttttaa taccatacca gcttcgcccc
    15841 cataagggta atcatctacc cttctatgtc tatcattgct aaaatctctc agattatgaa
    15901 tataaatatc tatcaatcct ttatcttttg ctcttttgat aatagaataa tcaaaagggc
    15961 caataaacat ctctggaaat acagttaaaa tatctattct catatagttc aaaataaacc
    16021 aaataaatta gcattaatta aattaataac ttcgcccaga tcttcggggt tatgcaaaaa
    16081 atctttacca tctacattta ttataagttt tttacccaga tcataagtat ttatccaatt
    16141 ttcatatttt tcatttaagc tttgcagata atctaaacga atagaagtct catactctct
    16201 accacgaatt tgaatctgtc taactaaagt agatacatca gcttgtaaat aaatcagcaa
    16261 atctggtggg cgtaaaaaag aaatcattaa atcaaataaa tctttataag tatcgaaatc
    16321 tctcttttcc atcaacccca tttcaaataa atttaaagca aaaatatgag catcttcgta
    16381 gattgtacga tcttggacca ctcgtaattg gtttttttgt atttccattg tttgtttaaa
    16441 tctgctacag aggaaataaa tttgaagatt aaaggaccat cgcttcatat cattataaaa
    16501 acttatgatg taaggattat tatcaatatt ttcataaata gcatttaaat ttaattgttt
    16561 ggctaaaagc tcagtcaatg tagtttttcc acttccaata ttacctgata ctgctatgtg
    16621 taacatgatt ttttaatata ttaatttaat gtaaaaataa taaaaaaaaa taataaagca
    16681 aattaaatga atatatattc atttaattat attaattata tctaattata tttataatat
    16741 tttaaaataa tattttaaaa tattaattat gattttaatg aatattataa tattttaatt
    16801 ttcttatatt tgcacaatta aattattttt gggaaacaat ttcatgaatt tttcaacata
    16861 tctacttgta ggaataatta tagcttttat tgttttgata gcgtctatat ttaaacgctt
    16921 gaatgtccca cttatcctta tagcgttagc tataggtatt ttttttggaa gtgacgtaac
    16981 tggattgatt tattttgatg atgcacatct aacgaagcaa ataacggata tagcattgat
    17041 gtttatttta tttgccggtg gcttcgaaac taaacttaaa gatttgaaaa ctgtaatgac
    17101 tcctgcttta ttattgtcta gtataggtgt tatattaact ggtctaataa gtgctttatt
    17161 tttctcatta atttttaaat gggattttat tcaatcatta ttattatgcg ctattatatc
    17221 atctacagat gcgtctgcta ttttttctat tttacgtaaa agtgatgttg atttagaagc
    17281 cacatcattg actaaattag agtctgccac aaatgatcct atggctatta tttttactac
    17341 ttttttgata caattagttt caggtgttaa ttttagtact ttttctacgg taattatttt
    17401 tttatggtta tttatcggtg gtatagttat aggcatcttg ataggttttg taggtaaatt
    17461 tatttttaaa agattaaaag atctcgatgc tggttattat catttgctta ttttaggatt
    17521 gatattcctt agttttggtt ttgcggaatt atgcagggct aatggcttga tggcagtatt
    17581 ttttactggt ttgattttag gtaatactaa actaccatat aatagtcgca tagcttcttt
    17641 tacagatact ttatcattta taactaacgt tattatatat gttttgttag gtcttttagc
    17701 atttccaagt aaatttccag atttatgggt gtatggttta gtattatttt taatattaac
    17761 tcttgtagcg agacctataa ctgtcttttt attgacttcg tttaccaaac tttcaacaaa
    17821 gaaaaaagtc tttgtaagtt ggagtggtgt tagggggaca gtacctatag ttttagctac
    17881 atatcccata gtagcaggat tggatccaca gcatcagatt tttaatattg tttttattgc
    17941 tgtgatatta tctattgtaa tacaaggagg cagtatgaat aaagtatcca cttggttaaa
    18001 attagctaca aaaagaaaaa atactgtaca aaatcttatg gaactagtta ctgtgcatga
    18061 tacagattat gatttaatag aaatatttat tgatgaccaa aaatacatag gtgagtgcca
    18121 aatagctgat ttgaagctac catcaaatag tactataact atgattaata gaaataatca
    18181 agtaatagca ccctcaggac agactattat aatgccagga gatacattat ttgttttagt
    18241 caaaaaagaa aatattgaaa tagtaattga tagaatatta aataaattta ataaaattaa
    18301 tgaaatataa aatttctaat aataaaatta agaacccttg atctatcaat gaatattaca
    18361 ttttaaataa tgagtgatca aataattaaa aaattttcta aattttttaa ttaacatttg
    18421 tttaaatttt tcaagtttat atgttaaatt atttttatat ttttgcaaaa attttaggca
    18481 atgaaaaaaa ttgtagaaaa tactagcaaa taccttgcta tgagtaagga agagaataaa
    18541 gaattcttag ataaccttta ttctctaatg aaaaaattag aagctgatgt agtcgattat
    18601 caaggtctga aaatactcag tgccccacca ctatgcccag
//
LOCUS       DAOPRN010000038        16803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_2884_length_16803_cov_106.564067, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000038 DAOPRN010000000
VERSION     DAOPRN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16803
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2884_length_16803_cov_106.564067"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PKI65_05485"
     CDS             complement(<1..251)
                     /locus_tag="PKI65_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77927.1"
                     /translation="MRKVLLFIVSFWLLYFMQVKAQNVGIGSSLFTPDASAGLEIQFS
                     DKGLLIPRVSLTSTSSAAPITSPATSLLVYNTATTGDVTP"
     gene            complement(566..1450)
                     /locus_tag="PKI65_05490"
     CDS             complement(566..1450)
                     /locus_tag="PKI65_05490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOB77928.1"
                     /translation="MLRVYVAIIITMLIWGSTFVFSKILLTAGLMPHQLIFLRTLLGG
                     IALLLAIKIKKLSIKINKEDYKKLLLLALFEPFLYFIGENGALLYTSPTLVSLFIAMI
                     PLFIQITMFFLAKEPLKLNQIIGTLITIFGIILAIGGSGKLSEISIEGVLLLLLAIFS
                     AVFYNYYLSSLIKKYNSLLIVMWQTVIGAIYFLPFVLFSGKELLTMQWNARYITYLLI
                     LSILATGLAYILYAYVVKKIGVNKTSIFANLIPIITLTISIIMGTEKFHYTKLISVII
                     VIMGIYISQTKIEFKHKS"
     gene            complement(1631..2995)
                     /locus_tag="PKI65_05495"
     CDS             complement(1631..2995)
                     /locus_tag="PKI65_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77929.1"
                     /translation="MIKSEFFDIIIKSINRYYDINHKEFLYSFNEKPHIAIKVNPYKK
                     INQLFDLLHISDEVSWCSDAYYLSDRPDFYKDPLLYAGAYYVMDPASMFIDYIFKNLQ
                     LSNDIKVLDIAAAPGGKSIIVNSNLSNNAVLWSNDTNSNRAKTLNYNLIKWGRANNIV
                     SNAKISDFHSLQNQFDLVILDAPCTGSGFYRKYENWQEHFSIEYIKRCANRQKEILKD
                     LIPIIPLYGYLIYSTCSYTSEENEDIAKWLISHGFEEVHLSIPDQWGIIKSKYGYRFF
                     PHLTKSEGFYYVILRKIENKQSKFQNFNYNKKNQSSIRLQAPPHIDFENFIDFNDEIE
                     LKKIRQNISLISSSIAKWMDWKGFFFNGKVLNVGTTFIHENKKIPSPQFALSIYTSNS
                     IPQLHLNISDALSYLQKKSFKLNTDKGIYLVKYLNLALGWIKIIDDDRFNNYFPVDWR
                     IINM"
     gene            complement(3108..4049)
                     /gene="arcC"
                     /locus_tag="PKI65_05500"
     CDS             complement(3108..4049)
                     /gene="arcC"
                     /locus_tag="PKI65_05500"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOB77930.1"
                     /translation="MKKLIMAALGGNALLRNDQKGLYHEQIQNATDTCENLLDIIMQG
                     HNLVITHGNGPQVGNVLLQNEAGESKYGISALPMHVCVAETQGHIGYLIEQAFKNVLI
                     KHGIDKNIISIITQVIVDKNDPAFKKPSKPVGPYYNKEVADQLTEKFGWTFSEDPRGR
                     GYRRVVPSPKPIEILNWNIIKELAERGNIVIATGGGGIPVIKNKDGTLLGVDAVIDKD
                     LAAAALAVTIKANEFFILTDISNAYLNFNKPNQVKLGKVTVNEIKKYLEEGHFADGSM
                     GPKIKAGLYFLENTGNNVLITEANSLKDPDAGTRIIK"
     gene            complement(4076..6793)
                     /gene="ppdK"
                     /locus_tag="PKI65_05505"
     CDS             complement(4076..6793)
                     /gene="ppdK"
                     /locus_tag="PKI65_05505"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOB77931.1"
                     /translation="MEKKKRVYVFGNKKAEGAANLKDLLGGKGANLAEMDLLGLPVPP
                     GFTISTEACTEYYTLGKEKLFEILKNEVEHGLKHIETILGADFGDPDNPLLVSVRSGA
                     RVSMPGMMETILNVGLNDITREGLIKKTNNPRFVYDSQRRLIQMYSDVVMEKAAGIEP
                     EEGKGIRQLLEAELEKLKEQKGVKEDIELDAEDLKKLVDIYRAKVLEVLGKPFPEDPM
                     EQLWGAIFAVFQSWNGKRAVSYRRIEGIPDDWGTAVTVQAMVFGNMGEDSATGVAFTR
                     NPATGENMFYGEWLPNAQGEDVVAGIRTPYPLNEATKTEYNKHLPSLEKTMPEIYQQL
                     VDIRNILEKHYRDMLDIEFTIQERHLWMLQCRVGKRNGTAAVRMALDMLNEGLIDEKT
                     ALLRVKPIQLDEMLHPLLDPASEAKAKVIAHGLPAGPGGAYGKIVFTSNDAVEWAAKG
                     EKVILVREETNPEDIDGMRAAEAILTARGGMTSHAALVARGWGKCCIVGASDIEINLQ
                     KKEMITPAGIYKEGDMISLNGTKGLVYEGQLDTISPSENPYFIKFMDLVDKYRVLKVR
                     TNADTPQDAKIALKFGAEGTGLFRTEHMFYGKGGETPLFILRKIILSTKEEERRKSLD
                     ELFPFVKEDIKNTLEIMDGLPVTIRTLDPPLHEFIPKNQNARESLAKELNITIEELNN
                     RVDQLHENNPMMGHRGVRVGITYPEITEMQVRAILEAAAELIKEGKNPKPEIMIPVTA
                     IETELQNQLEIVEKVYEEVKIKYNMNEIPYEFGTMIEIPRAALRADDMAKYAQFFSFG
                     TNDLTQMTYAFSRDDVGSFLQEYLDKNIIKFDPFQIIDRDSVGELMKIAVERGKSTRP
                     DLHLGICGEHGGEPSSVEFCHMIGLTYVSCSPFRVPIAKLAAAQAAVKE"
     gene            6918..7436
                     /locus_tag="PKI65_05510"
     CDS             6918..7436
                     /locus_tag="PKI65_05510"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOB77932.1"
                     /translation="MKKSFEELEKLQIEQLILYGKEYCDAIEHCEEYEFINLLNFLDI
                     LLPILYFHGSFVPFVDDCDLGYAERTVTEETYTIHYNKLTKIFNEHLKLDLSENEGIE
                     KWINSFSISEYLCDIYQDIKDVVCLYDKNTYEAKKCAIHLAKIWFVERWGGELLRVLL
                     AIHYLLFEKIYH"
     gene            8276..10051
                     /gene="recJ"
                     /locus_tag="PKI65_05515"
     CDS             8276..10051
                     /gene="recJ"
                     /locus_tag="PKI65_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOB77933.1"
                     /translation="MQIKRWRFKPKGDDNIITELNQHLGIGMIASQLLVQRGITNIKE
                     AQVFLRPRLDDLYDPYLMKDMDNAVKRIAKALNDKEKILLYGDYDVDGITSVANLYLF
                     LSRFTNKLDYYIPDRYTEKSGISEQSIEFAIKNGYSLIIASDCGTKEIEIVEYANKNN
                     IDFIICDHHKATDTLPNAVAVLNPQRKDCNYPFKDLSGCGVGFKLIQALAKMYNIPNS
                     ELTGLMQLLALSIAADMVPVIDENRILAKFGLKYINENPLPGIEAVLKVSGIKRQPDN
                     IYHKDHFFSKLITLSDLVFLVAPRVNAAGRIQNGRDSVDLLVCNDITKADILAEQINN
                     YNEERKNIDFKATNEALEMIENYEISNFATILYNPNWPKGVLGIVAARLTEQFHRPTI
                     ILTWSHEDQLISGSARSTKNFNLYEALDQCSDLLENWGGHTYATGISLKEENLEEFTK
                     RFVEIAEKLLTENMLISEIEIDAELDFNDITKKFVRILNDFEPYGTGNPQPIFYSKNV
                     YDAGGYTKIVANNHLKLFLSQPHYKYGPGFPAFAYNSVEYYDHIVAGKPIDIIYNIEE
                     NDWVGTNTIIQLNIKDIIKKEPDYE"
     gene            10044..11048
                     /gene="trpS"
                     /locus_tag="PKI65_05520"
     CDS             10044..11048
                     /gene="trpS"
                     /locus_tag="PKI65_05520"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOB77934.1"
                     /translation="MNNEIVVSGIRPTGYLHIGNYFGAIRNFVEMQNIYSCYFFVADY
                     HSLTTNPNPKDLHNTSLHLLAELLACGLNPELATLYLQSEVPEVIELYLILNMNAYLG
                     ELMRVTTFKEKARKQPENVNAGLLTYPTLMAADILIHKANKVPVGKDQEQHLEMTRDF
                     AARFNRIYNIDYFPLPVAFDFSGDLIKIPSLDGRGKMGKSEDPNSTIFLIDNDEIIRK
                     KIMRAVTDMGPTEKNQPMSEPISNIFNIMSLVSDPDTMQYFIDKYNDTSIKYSELKKQ
                     LADDMIAFIAPIREKINEIYSNKNLLYQVIDLGKQKAQKNARNTINDIRQFIGFRKLY
                     "
     gene            11132..12874
                     /locus_tag="PKI65_05525"
     CDS             11132..12874
                     /locus_tag="PKI65_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77935.1"
                     /translation="MKSKFFSISFLAIVLLFTSCDIQKMAKKYDTVKYETTPQVLETH
                     GGKINVSVKGNIPPKYFGKKVVVDFTPVLKWDGGSYTCKSIKIGGEKSEGSDVKINKK
                     GGGSFTWSDVITYQPEMNSSELVVNAKATKGKKTVEFGEVKLADGVIYTSTRIGRDED
                     LALAEHGYKKEVIVSKTATLYFDYNSSKLYDNQKLNKLEENKKLVQELKDFIALNWKI
                     NNIEINAWASPEGEEQYNANLSNDRAAAAKKYIESYIEKLDKEIAKNQKKKYNEVKRE
                     APLTINGKGEDFDGFMKAIEKSNLPEKQAIINVIKSQPDKAKREREIKNMSVVYAEVE
                     AILEPLRRAEIVVSCYAPKRTDDDIARLSLTAPDSLKAEELLYAATLTDDINNKLKIY
                     KAGTEIYPSDWKFWNNTGYILLSQHNISEATPYIEKANTLSPNNPVVLNNLGVIAAWN
                     KQYDKAKEYYEQAKSEFDVNYNLGVLKIVEGDYAGALKLFSGKSCRYNIALAQLLSTD
                     YTSAARTLECIPNKTPEVYYLMAIVGARTGNTSMLIDNLKQAVKDPALKSQAKTDREF
                     LKYYNNSDFQAIIK"
     gene            12985..14181
                     /locus_tag="PKI65_05530"
     CDS             12985..14181
                     /locus_tag="PKI65_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOB77936.1"
                     /translation="MKRLFGLIFVMIISTFMLSAQVDTTQKKEGFQFTIIKENPTTSV
                     KDQYKSGTCWAFSTLSFLESELLRTGKGEYDLSEAFIVRNTYIEKAIRYVRFQGKTNF
                     SPGGGFRDITYVINKYGIVPEEVYTGLNYGTDKHVHAELDAVLKAYVDQIIKNPNREL
                     TTAWLDGYIGILDAYFGKVPEKFIYKGQEYTPITFAQSLGLNMDDYVEISSFTHHPFY
                     EQFILEIPDNWLHGMVYNVPLDEMMRIIDYSIENGYTVAWGSDVSEKGFKWSKGIAVV
                     PDETLPDLTGTEKERWEKLTDAEKQKQIYAIDGPVKEKIITQELRQIAFDNYNTTDDH
                     GMHITGIAKDQKGNIYYKVKNSWGLSGPYEGYFYASKPFVAYKTTGIMINKNSIPKDI
                     RKKLGL"
     gene            14292..15758
                     /locus_tag="PKI65_05535"
     CDS             14292..15758
                     /locus_tag="PKI65_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOB77937.1"
                     /translation="MSGLSNTNDARSFVKSLFQFSIGQWIAAFITFITTPIITWLIIP
                     NEFGKAAMFTLAFNLILNIVLLGADQSFVRFFYERKEIKRRDLLWESLLPSLILGIIT
                     FIIIGIFWQELSFLLFGDANHFTPILLLDLTIFVAIIERYAMLLVRMKQRGKAYSMIK
                     VINSLANAIFTILYALLVAKTFYAVIIGLFFSHVFSATVAIIYEKDIWFGKFKINLNS
                     IKSIINYGLPFVPTAVIIWIFQSIDRIAIRNYCDFAEVGLYSAAFKIVAVMSLLQNSF
                     ANYWVPVSYETYEKNPNNTAFFEKISLFISAIMFACGMLLVIFKDVIFLLLESSYRGA
                     AGLTAFLILMPIMDTVAYVTTIGINFKKKTYLYIIRAVISATLNVIGNIILVPKYGAE
                     GAALSTGISYVVYFFIGTYYSQKLYPVDYHLNKFYTCAFIFLIVAFINTFVPYLLLQI
                     ISAIIGLIIVLFVYKEQIIYLFNFSKNEFIRLKSKKRY"
     gene            complement(16174..>16803)
                     /locus_tag="PKI65_05540"
     CDS             complement(16174..>16803)
                     /locus_tag="PKI65_05540"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOB77938.1"
                     /translation="PGGNPSTSTQTNPIVTYNNSGIHIVTLIAYNGSLNNSTSQNIIV
                     YPKPQLSTTIQNVSYYGGNDGAIYLSVAGGLQPYSYQWNTGATTQNLINISAGTYYVT
                     VTDSRSCKNNTSATVTQPSDIIDYNLSNLAIIFPNPASDYLNINFDYIPQNNQIKIKI
                     ISSIGKIAYESDEVFSSMLSIPIYNLTTGTYIFSVLLEDNWYYSLFYKQ"
BASE COUNT         5980 a         2502 c         2454 g         5867 t
ORIGIN      
        1 ggcgtcacat ctccagtagt agctgtatta taaactaata aactagtagc aggagaagtg
       61 ataggtgctg ctgagctagt actagtaagt gatacgcgtg gtattagaag tcctttatca
      121 gaaaactgaa tttctaaacc agcagatgca tcaggcgtga aaagactaga tccaattcct
      181 acattttgtg ctttgacttg cataaaataa agcaaccaaa atgaaacaat aaacaataaa
      241 acctttctca tatattatat attttatttt gtttatattt ttagctaaat taattatttt
      301 gatatataaa aattttatta aaaaatatta ttattgaaat atagtttgag ttaataattt
      361 acatttaaat ctttattaat caaacgtcta acataatata taatctaaaa attagataat
      421 tgtaataaac aaataatata agcataaact gggacactga ttatacatac ttattgctaa
      481 tttaaatata cattaaacaa gctagtttat aaaaaaagat atttactttt tacaaaatca
      541 tcaactaaac atttaactaa caatatcaac ttttatgttt aaattcaatt ttggtttgag
      601 aaatgtatat acccataatt actattatca cactaatgag cttagtataa tgaaactttt
      661 ctgtgcccat aataatagaa atagtaagag taattattgg tattaaatta gcaaatatag
      721 atgttttatt tacaccaatt tttttaacta cataagcata taatatgtaa gcaagaccag
      781 tagctaaaat agataaaata agcaaatatg taatatatct agcattccat tgcattgtta
      841 ataattcttt tccgctaaat aaaacaaatg gtaaaaaata tattgcacct ataactgttt
      901 gccacattac tattaataaa ctattatatt ttttaatgag tgaagataaa taataattat
      961 aaaaaacagc agaaaagata gctaataaca ataataaaac cccctctata ctgatttcac
     1021 ttaatttgcc actaccacct attgctaaaa ttataccaaa gatcgtaatt aatgtaccga
     1081 tgatctgatt taactttaaa ggttcttttg ccaaaaaaaa catagtgatc tgaataaaaa
     1141 gtggtatcat agcaataaat agcgatacaa gtgtggggct agtatataaa agtgcaccat
     1201 tctcaccaat aaaatataaa aaaggttcaa agagagctaa taataaaagc tttttgtaat
     1261 cttctttatt gatttttata gataattttt tgattttaat agctaataaa agagcaatac
     1321 ctcctaataa agttctcaga aaaattaatt gatgtggcat caatccagca gttaataaaa
     1381 tcttagaaaa tacaaaggtg ctaccccata ttagcatagt tatgatgata gctacatata
     1441 ctctgagcat acttaaaaat tattagtaac aaaaataaga ttttttacat taatataatt
     1501 tacaactaac aacctacaac aaacatttta ctcgattact ccatttgctc aactattaaa
     1561 ctgatttact tcatattata tttgagatat ttgtgtaatt aactaataat ctatgtgtta
     1621 gtgtatctta ttacatattt attattcgcc aatctacagg gaagtaatta ttaaacctat
     1681 catcatctat aattttaatc catcccaatg ctagattgag atattttacc aaataaatcc
     1741 ctttatctgt atttagttta aatgattttt tttgtaaata tgatagagca tctgatatat
     1801 tcaagtgtaa ctgtggtatg gaatttgatg tatatataga gagtgcaaac tgcggtgaag
     1861 gtattttttt attttcgtgt ataaatgttg tacccacatt taatactttg ccattaaaaa
     1921 aaaagccctt ccagtccatc cattttgcta ttgatgagct aatcaaacta atattttgcc
     1981 ttattttttt taattctatt tcatcattaa aatcaataaa attttcgaaa tctatgtgag
     2041 gtggagcttg tagtctaata gaagattgat tttttttatt gtagttaaaa ttttgaaact
     2101 tagattgctt attttctatt tttctgagaa taacataata aaaaccttcg gatttagtaa
     2161 gatgtggaaa aaatctatag ccatatttag attttatgat accccattga tcgggtattg
     2221 ataaatgaac ctcttcaaat ccatgagaaa tcaaccattt tgctatatct tcattttctt
     2281 cggaagtata tgagcaggtg ctataaatca aataaccata caaaggaatg ataggtatca
     2341 aatctttcaa aatttctttt tgtctattgg cacatctctt tatgtattcg atggaaaaat
     2401 gttcttgcca attttcatat tttctataaa agccactacc agtgcagggt gcatctaaaa
     2461 taacaaggtc aaattgattt tgaagactat gaaaatctga tattttagca tttgatacta
     2521 tattattagc tctgccccat tttattagat tataattcaa tgtttttgct ctgttagagt
     2581 tagtatcatt agaccacaaa acagcattat tggataaatt agaatttaca attatagatt
     2641 taccaccagg agcagcagca atatccaaaa cttttatatc atttgataac tgtaaatttt
     2701 taaaaatata atctataaac atcgaagcgg ggtccatcac ataataagca cccgcataca
     2761 ataaaggatc tttgtaaaaa tcgggtctat cagataaata atatgcatca ctacaccagg
     2821 atacttcatc agaaatatgc aaaagatcaa ataattgatt gattttttta taaggattta
     2881 ctttaatagc aatatgaggc ttttcattga aagaatataa aaattcttta tgatttatat
     2941 cataatatct attaattgat ttaataataa tatcaaaaaa ttcagatttt atcataaaaa
     3001 ttgataaata ataatttcac atattacaaa gatagaagat ttaaaaaaaa tatgtacata
     3061 ttttttttca cactataata tattacaaat tgtatcgtaa aaagttatta ttttataatt
     3121 ctagttccag catcagggtc ctttagacta ttagcttcag tgatcaaaac attattacct
     3181 gtattttcta aaaaataaag accagctttt attttaggtc ccatactacc atcagcgaaa
     3241 tggccttctt ctaaatattt tttaatttca ttaacagtta ctttgcctag tttcacttga
     3301 ttaggtttat taaaattgag ataagcatta gaaatatcag taagtataaa aaactcatta
     3361 gctttgattg tgactgctaa cgcagcggcg gcgaggtctt tatcaataac agcatcgaca
     3421 cctaacagag tgccatcttt attttttatg acaggtatac cacccccacc agtagcaatt
     3481 actatatttc ccctttcggc caattctttt attatattcc aatttaatat ttctataggt
     3541 ttcggacttg gaactaccct tctataaccc ctaccacgag ggtcttcact aaaagtccaa
     3601 ccaaattttt cagttaattg atctgctact tccttattat aataaggtcc tacaggctta
     3661 gagggttttt taaaagcagg atcattttta tctacaatta cttgagtaat aatactaatg
     3721 atatttttat cgataccatg cttaatcaaa acatttttaa aagcttgctc aatcaaataa
     3781 ccaatgtgac cttgtgtttc tgctacacat acatgcatag gcaaggctga tataccatat
     3841 ttactttcgc ctgcttcatt ttgtaataaa acattaccta cttgtggacc attaccatga
     3901 gtaattacta aattatgacc ttgcataata atatctaaca aattttcaca tgtatcagtg
     3961 gcattttgta tttgctcatg ataaagacct ttttgatcat ttctgagtag cgcatttcct
     4021 cccaacgcag ccattattaa ctttttcata acacaagtgt gcttctaaaa aaaaatcatt
     4081 cttttacagc agcttgagca gcagccaatt tagcaattgg cacacggaaa gggctgcagc
     4141 tgacataggt tagaccaatc atatggcaaa attccacaga tgatggttcc ccaccgtgct
     4201 ccccacaaat acctaaatgt agatcaggac gagtagattt accacgttct acagctattt
     4261 tcattagctc acctacacta tctctatcta ttatctgaaa tgggtcaaat tttataatat
     4321 ttttatcaag atattcttgc aaaaatgaac ctacatcatc gcgcgaaaaa gcgtaagtca
     4381 tctgtgtaag gtcattagta ccgaagctaa agaattgagc atatttagcc atatcatcag
     4441 cacgtaacgc ggcgcgaggt atctctatca tggtaccaaa ctcataaggt atctcattca
     4501 tattgtattt gatttttact tcttcgtaca ctttttctac tatttctaat tgattttgta
     4561 attcagtttc tatggctgta actggtatca taatttcagg tttaggattt ttaccttctt
     4621 taatgagttc cgctgcagct tctaaaatgg ctctcacttg catttctgtt atttcaggat
     4681 atgtgatacc tactctcaca cctctatgtc ccatcattgg attgttttca tgaagttgat
     4741 ctacacgatt atttaactct tcgatggtaa tatttagctc tttagctaaa ctttctcgcg
     4801 cattttgatt tttaggtata aattcatgga gtggaggatc taaagtacga atagttacag
     4861 gcaaaccatc cattatttct agtgtatttt taatatcttc ttttacaaat ggaaaaagtt
     4921 catctagtga ttttctacgt tcttcttctt tagtactaag aataattttt ctaagaataa
     4981 ataaaggtgt ctcgccacct ttaccataaa acatatgttc agtcctgaaa agtccagttc
     5041 cttcggcacc aaattttagt gctatttttg catcttgtgg agtatcggca tttgtacgaa
     5101 cttttagcac tctatattta tctactaaat ccataaattt tataaaatat ggattttctg
     5161 aaggactaat agtatctaat tgtccttcat ataccaaacc tttagttcca tttagtgaaa
     5221 tcatatcacc ttccttgtat atgccagcag gagtgatcat ttcttttttt tgtaaattaa
     5281 tttcgatatc gctagcgccc acaatacagc attttcccca accacgagct actaacgcag
     5341 catgactagt cataccacca cgagctgtga gaatagcttc tgctgcacgc ataccgtcta
     5401 tatcttctgg atttgtctct tcgcgtacaa gaataacttt ttcacccttt gcggcccatt
     5461 ctacagcatc attagatgta aaaactattt taccataggc accacctgga ccagctggca
     5521 atccatgtgc tattacttta gctttagctt cacttgctgg atctaataat ggatgaagca
     5581 tctcatctaa ttgtataggc ttcaccctta ataatgcagt tttttcatct attaatccct
     5641 cattgagcat atccaaagcc atccttacag cagcagtacc attgcgtttg ccaactcgac
     5701 attgcaacat ccataagtgt cgttcttgaa tagtaaattc aatatcgagc atatctctat
     5761 agtgtttttc taagatattt cttatatcta caagctgttg atagatctcc ggcatagttt
     5821 tttctaacga aggcaaatgt ttgttatact ctgttttagt agcttcattt agaggatatg
     5881 gagtacgaat accagctaca acatcttcgc cttgtgcatt tggtagccac tcaccataaa
     5941 acatattttc acctgtagca gggttacgag taaatgcaac gcccgtagca gagtcttcgc
     6001 ccatattacc aaagaccata gcttgtactg tgacagctgt gccccaatca tcaggtatac
     6061 cttctattct tcgataagac actgctcttt taccattcca actttgaaaa actgcaaata
     6121 ttgcacccca gagttgttcc attggatcct caggaaaagg ttttcctaaa acttctaata
     6181 ctttagcacg atatatatct actaattttt tcaaatcttc agcatcgagt tcaatatctt
     6241 cttttacacc tttttgttcc tttaattttt ccaattctgc ttctaacaat tgtcgaatac
     6301 cttttccttc ttctggttct ataccagctg ctttttccat tactacatca ctatacattt
     6361 gtattaaacg gcgttgtgag tcatatacaa aacgtggatt gttagttttt tttatcaatc
     6421 cttcacgagt aatatcgttt aatccaacat ttaatattgt ctccatcata ccaggcatag
     6481 atactctagc acctgaacgc accgatacta acaaaggatt atcaggatct ccaaaatcgg
     6541 cacctaatat cgtctctatg tgtttcaagc catgctctac ctcatttttt aatatttcaa
     6601 ataatttttc cttgcccaat gtataatact ctgtacaagc ttcagtagaa atagtaaaac
     6661 ctgggggcac tggaagccct aagaggtcca tctcagcaag atttgctcct ttgccaccaa
     6721 gcaaatcttt caaattagct gcaccttctg cttttttatt tccaaataca taaactcttt
     6781 tcttcttttc catactatta tatctttttt attaattatt tttattgcaa agttaatttg
     6841 tttttttgaa ttatatgtat aatcaaaaat tttaataatt ttataattat ttatacattc
     6901 gtaaaaatta aaatactatg aaaaaaagtt ttgaagaatt agaaaagttg caaatcgagc
     6961 aattaatttt gtatggaaaa gaatattgtg atgctataga gcattgcgaa gagtatgagt
     7021 ttataaatct tttaaacttt ttagatattt tattaccaat tttatatttt catggttctt
     7081 ttgttccttt tgtagatgat tgcgaccttg gctatgcaga aagaactgta actgaggaga
     7141 catacactat tcattataat aaactcacaa agatttttaa tgaacatttg aaattggatt
     7201 tatcagaaaa tgagggtata gagaaatgga taaatagttt ttctatatca gaatatctat
     7261 gcgatatata tcaggatatt aaagatgttg tttgcttgta tgataaaaac acctatgaag
     7321 ctaaaaaatg tgccatacat ttagcaaaaa tttggtttgt cgaaagatgg ggcggtgaat
     7381 tattgagagt actattagca attcattatt tactttttga aaaaatttac cactaatact
     7441 tactattaca acgctttatt taaaacttat aagcataaac tcttttgaat cttaaattat
     7501 agtaaacaca acttaatata ataacttaat aaaaatatat tgcaagtttt gaattattaa
     7561 ataaaatatt taaatatcag catttctatt caataaaatt atagtaatca gtaaaaaaat
     7621 aatataataa ttttaggtag atgaccatag tttatattta ctttataaat agatctaaaa
     7681 aaaaaatatg gtaattacaa agaaaataat aaaattttaa aaattcatgt ttgttatact
     7741 taaaggcggt tcattaatat ttcatatata aaacaccaat actcatccca tgcccatctg
     7801 tacgaggatg cgaggtttaa aaagtctacc aatgtctgat tctatttcat ttgataaata
     7861 tttatgtata attacaaatc atgaatcatc acaaccaaac gatacaatat tttatttaaa
     7921 atattgtata aaatacttta agtataacga atgataattc tcattcagct tcacccttta
     7981 cgaagcttag taaaggtttc gaatctatag tgctttatta aatcattatt catagaatct
     8041 aactaaacat ccattttgta atttttaata aatatttatt aaatttttat ttataaaaat
     8101 gcaatacata tttaattaga aatattttaa ttaaaaaaaa ttatattttt agatagactc
     8161 tatggaatta gctctctaaa gtgcattcat tatttaataa aaattattaa taaaatattt
     8221 aatttatgaa aattaaaaaa attaaattaa atttgcaatt aagtaatatt ttaaaatgca
     8281 aattaaaaga tggaggttca agcccaaagg tgatgataat attataacag aattaaatca
     8341 acatttagga ataggtatga tagcttcaca gctattagtg caaagaggca tcaccaatat
     8401 taaagaggct caggtttttt taagacctcg tttagatgat ttatatgatc cttatttaat
     8461 gaaagatatg gataatgctg ttaaaaggat cgcaaaagca ctgaatgata aagaaaaaat
     8521 tttactatat ggagattatg atgttgatgg cattacttca gtagctaatt tatacttatt
     8581 tcttagtaga tttactaata aattagatta ttatattcca gatcgttata ctgaaaaatc
     8641 tggaattagt gaacaatcta ttgaatttgc tataaaaaat ggatatagtc tcatcatagc
     8701 atctgattgt ggcactaaag aaatagaaat agtagagtat gctaacaaaa ataatattga
     8761 ttttataatt tgtgatcatc acaaggctac tgacacttta cctaatgcag ttgcagtttt
     8821 aaatcctcag agaaaagatt gcaattatcc ttttaaagac ttatctggat gtggagttgg
     8881 ttttaaatta atacaagctt tagcaaaaat gtacaatatt cctaattccg aattgacagg
     8941 tttaatgcag cttttagctc tttccattgc tgcagatatg gtgccagtga tagatgaaaa
     9001 tagaatttta gctaaatttg gtttaaaata tataaatgaa aatccccttc ccggtataga
     9061 agctgtatta aaggtatcag gtataaaacg tcaacctgat aatatttatc ataaagatca
     9121 tttttttagc aaattaataa ctttatctga tttagttttt ttagtagcgc cgagggtaaa
     9181 tgcagctggt cgtattcaaa atgggcgaga tagtgtagat ctattagtat gtaatgatat
     9241 tacaaaagct gatatattag cagagcaaat taataactat aatgaagaac gaaaaaatat
     9301 tgatttcaaa gctactaatg aagccttaga aatgatagaa aactatgaaa tttcaaattt
     9361 tgccactata ctttataatc ctaattggcc taaaggcgtt ttgggtattg tcgcagctag
     9421 attgacagag caatttcatc gccctactat tatacttact tggtctcatg aggaccaact
     9481 cataagtggc agtgcacgtt ctacgaagaa ttttaatctt tatgaagctt tagatcaatg
     9541 tagcgattta ttagaaaatt gggggggaca tacttacgct actggtattt cattgaaaga
     9601 agagaatctc gaagaattta ctaagcgttt tgtcgaaata gctgagaaat tattgacaga
     9661 aaatatgctc atatctgaga tagaaattga tgcagaatta gacttcaatg atataactaa
     9721 aaaatttgta cgaattttaa atgattttga accctacggc actggcaacc cacaacctat
     9781 attttactca aaaaatgtat acgatgcggg tggttacact aaaattgttg ctaataatca
     9841 tttaaaactt tttctttctc agcctcatta taaatatgga ccaggttttc cagcctttgc
     9901 atacaattcc gttgagtatt atgatcatat tgtagctggc aaacccatag atattattta
     9961 taatattgaa gaaaatgatt gggtaggcac taatactatt attcaattaa atattaaaga
    10021 tattataaaa aaagaaccag attatgaata atgaaattgt cgtaagtggt attcgcccta
    10081 ctggatattt acatattggt aattatttcg gtgctatacg taattttgta gaaatgcaaa
    10141 atatatattc atgttatttt ttcgtagctg attatcatag cttaactaca aatcctaatc
    10201 ccaaagattt gcataacaca tccttgcatt tgcttgctga attgcttgct tgtggattaa
    10261 atccagaatt agcgactttg tatctacaaa gcgaagtgcc agaagtcata gagctttatc
    10321 ttatattaaa catgaatgct tatctaggtg agctgatgcg tgtaactaca tttaaagaaa
    10381 aagctcgtaa acaacctgaa aatgtaaatg ctggattgtt aacttatcct actttgatgg
    10441 ctgctgacat attgattcat aaagctaata aagtacctgt gggtaaagat caagaacaac
    10501 atttggaaat gacgcgtgat tttgctgcta ggtttaatcg tatttacaat atagattatt
    10561 tcccattacc tgtagccttt gatttttcag gtgatctaat taaaattcct agtttggatg
    10621 gtagaggcaa aatgggcaaa tccgaagatc ccaatagtac tatttttttg atagataacg
    10681 atgaaattat tagaaaaaaa ataatgagag ccgttactga tatgggacct acagaaaaaa
    10741 accaacctat gagcgaacca atttctaata tttttaacat tatgtctttg gtgtctgatc
    10801 cagatacaat gcaatatttt atagataaat ataatgacac tagtattaag tacagtgaac
    10861 taaaaaaaca attagcagat gacatgatag cttttatagc acctatacgt gaaaaaatca
    10921 atgaaatata tagtaataaa aatctactct atcaagtcat agacttaggt aaacaaaagg
    10981 ctcaaaaaaa cgctagaaat accataaatg atattagaca attcattggt tttagaaaat
    11041 tatattgatt tttataaaaa atttattatt tattttcatt taaaaaatat tatataaatt
    11101 tgcaatgtca aaaccctaaa attcaattat tatgaaaagt aaattttttt cgatttcttt
    11161 tttagccata gttttattat tcactagttg tgatatacaa aaaatggcta aaaagtacga
    11221 tacagttaag tatgaaacca ccccgcaggt tttagaaact catggtggta aaattaatgt
    11281 ttctgttaaa ggaaatatcc ctcctaaata ctttggcaaa aaagtagttg ttgattttac
    11341 accagtatta aaatgggatg gaggtagcta tacttgtaaa tctattaaaa taggtggtga
    11401 aaaatctgaa ggctctgatg ttaaaattaa taaaaaagga ggtggtagtt ttacatggtc
    11461 agatgtgata acttaccaac cagaaatgaa ctcttccgaa ttagttgtta atgctaaagc
    11521 tactaaaggt aaaaaaactg tagagtttgg agaagtcaaa ttagcagatg gggtcatcta
    11581 tacttccact cgcattggca gagacgaaga tctagcatta gctgagcatg gttataaaaa
    11641 agaagttata gtttctaaaa ctgctacttt atatttcgat tataatagtt caaaactcta
    11701 tgataatcaa aaactcaata agctagaaga aaacaaaaaa ttagttcaag aattaaaaga
    11761 tttcattgct ctaaattgga aaataaataa tatagaaatt aatgcatggg catccccaga
    11821 aggcgaagag caatataatg ctaatctatc taatgataga gctgctgctg ctaaaaaata
    11881 tattgaaagc tacatagaaa aattagataa agaaattgca aaaaatcaaa agaaaaaata
    11941 taatgaagtg aaacgagaag ctcctctaac aataaatggt aaaggagagg attttgatgg
    12001 atttatgaaa gctattgaaa aatctaactt acctgaaaaa caagctatca taaacgtgat
    12061 aaagtctcaa cctgataaag ctaaaagaga aagagaaatc aaaaatatga gtgtagtata
    12121 tgcagaagta gaggctattt tagaaccatt gcgacgtgca gaaatagtag tttcttgcta
    12181 tgcacctaaa cgtacagatg atgatatagc tagactaagt ctcacagctc ctgatagctt
    12241 aaaagccgaa gagcttctat atgctgctac tttgactgac gatattaata ataaacttaa
    12301 aatttacaag gctggcactg aaatttatcc atctgattgg aaattttgga ataatacagg
    12361 ttatatatta ttatctcaac ataatatttc agaagctact ccttatatag aaaaagctaa
    12421 tacattgtca ccaaataatc cagttgtttt aaataatttg ggcgtaatag ctgcatggaa
    12481 caaacaatat gataaagcta aagaatatta tgaacaagct aaaagtgaat ttgatgtaaa
    12541 ttataattta ggtgtattaa aaattgtaga aggcgactat gctggtgctt taaaattatt
    12601 ttctggtaaa tcttgtagat ataatatcgc tttagctcaa ctactatcta ctgactacac
    12661 tagtgcagct cgtacattag aatgtatacc aaataaaact cctgaagttt attatctaat
    12721 ggctatcgtg ggtgcacgta ctggcaatac ctctatgcta atagataatt tgaaacaagc
    12781 tgtaaaagat ccagctctaa aatctcaagc taaaacagat cgtgaatttt taaaatatta
    12841 taataatagc gactttcaag ctataattaa ataagatgtt ttcaatgtcc tacaaaaaaa
    12901 ggagggataa tccctccttt tttaattatt aaaaaaatat taagtatttt tgtaaaaatt
    12961 tgtaaaaata aaaaaattaa tgttatgaaa agattatttg gattaatatt tgtaatgatt
    13021 atttctacct ttatgctatc agcacaggta gatacaacgc aaaaaaaaga aggatttcaa
    13081 tttacaatta ttaaagaaaa tcctactacc tctgtaaaag atcagtacaa atcaggtaca
    13141 tgttgggcat tttctacttt aagtttctta gaaagtgaat tattacgtac gggcaagggt
    13201 gaatatgatt tgtcagaagc atttatagtg cgaaatacat atatagaaaa agctattaga
    13261 tatgtacgtt tccaaggtaa aactaatttt agcccaggtg gtggatttcg agatattact
    13321 tatgtcatta ataaatatgg aatagtacct gaagaggtgt atacaggatt aaattatggc
    13381 acagacaaac atgttcatgc agaattagat gcggtactaa aagcatacgt tgatcaaata
    13441 atcaaaaatc ctaacagaga attaactact gcatggctcg atggctatat tggaattttg
    13501 gatgcatatt ttggtaaagt acctgaaaaa tttatttata aaggacagga atacactcct
    13561 attacttttg ctcaatcact tggattgaat atggatgatt atgtagagat tagctctttt
    13621 actcatcatc ctttttatga gcaattcatt ttagaaatac ctgataattg gttacacggc
    13681 atggtatata atgtgccatt agatgaaatg atgagaatta tagattattc tatcgaaaat
    13741 ggatataccg tggcttgggg tagtgatgtt tcagaaaaag gatttaaatg gtctaaagga
    13801 atcgcagtag tacctgatga aaccttacca gaccttactg gtacagaaaa agaacgttgg
    13861 gaaaaactca ctgatgcaga aaaacaaaaa caaatttatg ctattgatgg acctgttaaa
    13921 gaaaaaataa ttactcagga gcttcgtcaa atagcttttg ataattataa taccactgat
    13981 gatcatggta tgcatattac tggcattgca aaggatcaaa aaggaaatat ttattataaa
    14041 gtaaaaaaca gttggggatt gagtggtcca tatgagggat atttttatgc gtctaaacca
    14101 tttgtagctt ataaaaccac aggtataatg attaataaaa atagtattcc taaagatatt
    14161 agaaaaaaat taggattata attgtttttt tataaattta ataaggtgtc attttttata
    14221 cattaattta taaatttggc accttttttt aattaatttt attaataata ttatttgact
    14281 ataaaaaaag catgtcagga ttaagtaata caaatgacgc gagatctttt gtgaaatccc
    14341 tattccaatt ttcaatagga cagtggatag cggcatttat tacttttatt acaacaccta
    14401 taatcacttg gctaattata ccaaatgagt ttggtaaggc tgctatgttt actttagcct
    14461 ttaatttgat cctaaatata gtattattag gtgcagatca gagctttgtt agattttttt
    14521 acgaaagaaa agaaattaaa cgaagagatt tactctggga aagcttatta ccaagcctga
    14581 ttttaggaat aataactttt atcatcattg gtattttttg gcaggagctt tcttttttat
    14641 tatttggtga tgctaatcat tttacaccta tattattatt agaccttaca atttttgtgg
    14701 ctattattga acggtatgct atgctattag tgagaatgaa gcaaaggggc aaagcttatt
    14761 ctatgattaa ggttattaat agtttggcaa atgcgatatt tactatttta tatgccctat
    14821 tagtggctaa aactttttat gctgtaataa ttggtttgtt tttttcgcat gttttctctg
    14881 ctactgttgc tataatttat gaaaaagata tctggtttgg aaaatttaaa attaatttga
    14941 atagtataaa atctattatc aattatggat tgccttttgt ccctactgct gtcataattt
    15001 ggatttttca gtcaatagat agaatagcaa taagaaatta ttgtgatttt gcagaagtgg
    15061 gattatattc tgctgccttc aaaattgttg ctgtaatgag tttactgcaa aatagctttg
    15121 caaactattg ggtaccagtt tcttatgaaa cttatgaaaa aaatcccaac aatactgcat
    15181 tttttgaaaa aatatctttg tttatttccg ctattatgtt tgcatgtggc atgttattag
    15241 tgatatttaa agatgtcatt ttcttattac tcgaaagttc gtatcgtggc gcagctggac
    15301 tcacagcttt tctcatactc atgcctatta tggatacagt agcttatgtt actacaatag
    15361 ggataaattt taaaaagaaa acatacttgt atattataag ggcagtaata tcagcgacac
    15421 tcaatgtaat aggtaatata attctagtcc ctaaatatgg agctgaggga gcagcactat
    15481 ctacaggtat ctcttatgtt gtctactttt tcattggcac atattattct caaaaattat
    15541 atccggttga ttatcattta aataaatttt atacttgtgc ctttattttt ctaattgtag
    15601 cttttatcaa tacttttgtt ccttatttat tgttacaaat tatttctgct atcattggac
    15661 ttatcattgt attgtttgta tacaaagaac aaataatata cctttttaat tttagtaaga
    15721 atgaatttat aagattgaaa tctaaaaaaa gatactaatt atatcctcac cattttcata
    15781 ggtgaaaata tacacatata taagttttta attttaaaaa caaagttaaa ttattttatt
    15841 gattacagtt aatttatatt atataattta attgaattag gcgtttagat tataaatgag
    15901 tgggcttttg tatgatcaat ttgcccatta gtaaactatt atatgaaaat ataatcatgc
    15961 gcccacaatt aatttcgacc tttagatctg aagataaaca aagagaaaga tatacctcct
    16021 ttcaggctaa aatccgattt aattaactct gacctaaagt gcaaggaaag agagacaacg
    16081 ggctttaact ctaaaaaact atgaatacaa aacttttagc atatataatt atatataaaa
    16141 aatataatat taccaataat ttaattttag tattcattgt ttataaaata aagaataata
    16201 ccaattatct tctaacaata cactaaagat atatgtacca gttgtcaaat tataaatagg
    16261 tatgctaagc atactagaaa aaacttcatc ggattcatat gctatttttc ctatactaga
    16321 tatgattttt atctttattt gattgttttg tggtatatag tcaaaattga tatttaaata
    16381 atcagaagct ggatttggaa atattatcgc taaattagac aaattataat ctattatatc
    16441 acttggttga gtaacagtgg cagaagtatt atttttacaa ctacgagaat ccgttacagt
    16501 aacataataa gtaccagcac ttatatttat aagattttgt gtagtagcac cagtgttcca
    16561 ctgatatgaa tacggttgta atcctccagc aactgataga taaatagctc catcgtttcc
    16621 tccataataa gaaacatttt gaatcgtcgt gcttaactga ggcttaggat agacaataat
    16681 attctgagaa gtactattat ttaaagaacc attataagca attaatgtaa ctatatgtat
    16741 tccactatta ttataggtaa ctattggatt agtttgtgta cttgtagatg gatttccacc
    16801 tgg
//
LOCUS       DAOPRN010000039        16768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_2892_length_16768_cov_90.246993, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000039 DAOPRN010000000
VERSION     DAOPRN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16768
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2892_length_16768_cov_90.246993"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PKI65_05545"
     CDS             <1..817
                     /locus_tag="PKI65_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77939.1"
                     /translation="KDNLTLFTTDAKTSRNSARANTAAGESLLDAVKKTNNLQKKKKL
                     LDEAIYYLNRAISIYPEYYAAHNILGEAYFMKKNYNEALNNALICAKLRPMELLPYKN
                     IFAAGINAYYDSSFDVANHAFTFLTNNSYFIMNQFSDSTYFTLQLALAFRYLNKIDSA
                     IIILENIIKKDTCYVEAFNQLAELHSMKPGGLETSEYYLLRGYNCDPNNAAILENLGT
                     LNVIKHNYSQALIYLKNVYNINPNNKNVIKNLAIAYQNLGMIDSAEYYANKLH"
     gene            complement(909..1547)
                     /locus_tag="PKI65_05550"
     CDS             complement(909..1547)
                     /locus_tag="PKI65_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOB77940.1"
                     /translation="MPVIFYNKIKEGLELGIWQITESIEELLKYLYLNKEEFDTLESF
                     KSEKRKKQWMSYRALIRSIVQADFIYRIYYDENNKPFLVNPPRAISVSHTDGYSAILI
                     NENINENRGLDIELISNQALKLKNKFLTIEEEKLLPKNDIKLYTTLFWSAKESVYKKI
                     NIKNIYLKSNINIEKIDKNNQIIKTKLIINENVTYINVFWLIFDSIVITYTD"
     gene            complement(1549..2589)
                     /locus_tag="PKI65_05555"
     CDS             complement(1549..2589)
                     /locus_tag="PKI65_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOB77941.1"
                     /translation="MKKINITLFFSLIAICLFSQIPYGYYDNAEGKSGQELKQALFNI
                     INSNVTTLSYNQLWNAYPLTDKKPNGKVWDIYSDIPGGIPPYEYTFIIDQCGNYNSEG
                     DCYNREHTVPASWFNDASPMYTDLFHVLPTDGYVNNRRSNYPYGNVGSASWTSLNGSK
                     VGSSITTGYSGIVFEPIDSFKGDIARIYFYMSTRYMNKINNWNGESFENGDLAPWAKN
                     LFLQWHQLDPVSTKEKQRNDSVFVIQHNRNPFIDHPEWVASIWGPSAHILDYNFVANI
                     YTTDDFIHIYFPNVNDDVQIKLYTIQGQLIYNDIAYNPTDINIPISFNRGLYVIIINN
                     KTNFFIQKLIIQ"
     gene            2836..3303
                     /locus_tag="PKI65_05560"
     CDS             2836..3303
                     /locus_tag="PKI65_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HOB77942.1"
                     /translation="MIQKITIITGGQSGVDRAAMDFVLDNPQLKIKCGGFCPKGRKAE
                     DGIIPQKYPLIECETDNYAVRTELNVKNSDGVLIIFKVKINDPGTTLTVELAKKHNKP
                     LYLCNIKQNNINEIRKWISENAINTLNIAGPRLSNDPEIYELTYDFLVQLFLV"
     gene            3642..4373
                     /gene="pepE"
                     /locus_tag="PKI65_05565"
     CDS             3642..4373
                     /gene="pepE"
                     /locus_tag="PKI65_05565"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HOB77943.1"
                     /translation="MKRNLLLISNSTMAGEVYLGWCKELITNFLKKFNIYEVLFIPYA
                     GVNLDSKSIEFSYNAYESKVQRVFDTDNIDINSIHHYSDPTNAVLNAKCIMVGGGNTF
                     HLVYELYKNNLMTTIREKSLCGIPYIGWSAGANLACPTIKTTNDMPIVFPKSFDALNL
                     IDFQINPHYIDHNPADHGGETREQRILEFLVVNKDMKVLGLREGTYLEIYNDNMWLKG
                     DKPARLFQFNTEPREIKPGENMIII"
     gene            4403..4945
                     /locus_tag="PKI65_05570"
     CDS             4403..4945
                     /locus_tag="PKI65_05570"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HOB77944.1"
                     /translation="MNKKIKFIVLSIIIIFYNSIKVNAQLPIDPDTKKIKYQEVVQEQ
                     GNKDTLYNRAIRWINSFFENPQGITKVRDPENGKIVGTYRMRMIDTLVDGSKTLSNTI
                     VICTFTIEAKDGRYRYTLDDFYVPAVSKMFLEKWMDKTDPYYLPKYDSYLKQVDEYVN
                     RFIKSLKEGMKPPIIKIDEW"
     gene            5125..5586
                     /locus_tag="PKI65_05575"
     CDS             5125..5586
                     /locus_tag="PKI65_05575"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOB77945.1"
                     /translation="MKKILVFLFLLVPILLHSQLYINTSYIPQQLVEDFLIGPGITVS
                     NVTHRGQLQQFAYFSNGNSTVLGMDEGIILCTGTASNIAQNGSSFMSNSLGGLGVTEL
                     DSIVSPYTTNDGAVLEFDFIPLDTLLTFYIFLEVKNIQNLYVLLLMMCLLF"
     gene            5571..7415
                     /locus_tag="PKI65_05580"
     CDS             5571..7415
                     /locus_tag="PKI65_05580"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOB77946.1"
                     /translation="MFAFLISGINPSGGMYVDKNIALVPGTDLPVAINTINPGVPGSG
                     FSASECISLSYSNLYINNNDSYQQPVFDGFTIPLVAEAKVIPCQTYHIKLCIADVTDH
                     VYDSGVFLKRNSFLTNLPELNIVTPYASPDTIYEGCAPIDLVFSHPEPVPYHTNIAIG
                     IGGTATNGVDYTYLSNIITIPADSTSVSHTIYAFADGIDEADEYLIINISGYCGLISS
                     DTIWIKDNPFFVTLNDTTICEDDVPLTLTPTIHDGQAPYNFIWNTGDDNENLIIYPDF
                     TSTYSVTVSDNLSCSASAEALITVNPLPDVYINPSDTSICKNEFITLTVYGSDFYVWS
                     DGSTDSSITVSPQTTTIYSVTGTDSSGCSNSALATVNIIESPSLNITASPPKLCSGES
                     SKLIVSGAQTYLWSTGETTSQIIVSPQITTSYTVTGSIQNCSDTKSITIEVIPNPEVN
                     ITASATHLLAGDIASLQAEGANEFLWYGDNSISCINCPTIEVSPLSSTEICVIGFNSI
                     CTDTACVFIEVDPCTLFIPNAFTPIKNSLNDVWQIFTPCPIDKGEIRIYNRWGKQIFY
                     TTDLYSSWDGTFEGELVPPGVYSFMIRYAYAHTNSIYYTRKGTITILY"
     gene            complement(7583..9046)
                     /locus_tag="PKI65_05585"
     CDS             complement(7583..9046)
                     /locus_tag="PKI65_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOB77947.1"
                     /translation="MKKLSFYLGLCLVIIGYTGIYAQNLDDAVRYSYLMPYGDSRFNA
                     VSGAFSGIGANITGISLNPAGLGVYKKSEISGSFSISNFLSNSKYLNNSNDDIKTVVS
                     LPSMGLVISSNFNTDKHDWKNLNFGLSYSKLADFNSRIMISADNPYSSIVDVYSVYAN
                     GFLPNQLNVFDTWLAFDTWLLDTIPGTPTAYEGIVTRGVRQDYLEINSGSMYEINLAI
                     AGNYDDKLYIGGSINFPIFSFERESTYSEEDVKDTIPDFKSLEVFDYLNTDGEGLNFK
                     IGFMYKPIQWFRFSLAFHSPTYFWLHDSYFRTMRTNVYNGIYTADSPRGEFNYRYYNP
                     MRAIIGLGFIFGENGFINAEYEWTDYTQNHFSTKKANTTFFDLNNSINNELTYGHNIR
                     VGTEWVLKPFAMRAGYAYYSSPYKNISNFDTHVIGLGFGYRENILIFDVGINYYLHND
                     QRYLYDPLVMEILNKPEPKANLSMNNILVSFTIGMKL"
     gene            complement(9073..>9609)
                     /locus_tag="PKI65_05590"
     CDS             complement(9073..>9609)
                     /locus_tag="PKI65_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77948.1"
                     /translation="PTQPANIKQPTQQQKPITQPTQPANIKQPSQQQKPITQPTQPAN
                     IKQPSQQQKPITQPTQPANIKQPSQQQKPITQPTQQQNTNQYQKPQESNPSKVTPSQT
                     QTPSRSYSRPSPNKNQPVQPTRYSSPPANNYSSPNRSNNSSPSYNAPSRSSSSGSSHS
                     NTPRSSGSGSAPSRGGRR"
     assembly_gap    9610..9709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9710..10811)
                     /locus_tag="PKI65_05595"
     CDS             complement(<9710..10811)
                     /locus_tag="PKI65_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77949.1"
                     /translation="MKTLNFLIACVVGLIFSGCTINNYYTDNTPYDDVYYNPQVHQYS
                     SAKAPNDQNYQSYENQQYDPTQAEPYYSETYTDSYGNTYITNNYYGDYYDFSYSSRIR
                     RFYYPELGFGYYAPWYTNMYWYTYDPWYFGISIYLTYPFWRPGFSFYWGYHPYNYYLG
                     WCYPYYSWGYPYGYWHGYHNGYWDGFWLGYYHGFYGSEYFYYNPFDVHSGTVYYGPRD
                     YISASTSGRQNRTINDNIPPASLNRTPASNLTFADRLSRVGYTPLEVKPQEITITKPS
                     SLINIDQNATLNDKLPNNLDTDKKPIQKPVTTSEQPIKPAIIDKPINEQKPITQPTQP
                     ANIKQPTQQQKPITQPTQPANIKQPTQQQKPIT"
     gene            complement(10877..12220)
                     /locus_tag="PKI65_05600"
     CDS             complement(10877..12220)
                     /locus_tag="PKI65_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77950.1"
                     /translation="MGLDPIFSFENILISLILIITIFSLIYFYSKVVTDNIFHKYLMY
                     GLGFKILMSIAFIFIYYFYYGGGDAYWYCYNTKCFVNAFFNHPGKISKIIFTGNMNYN
                     DFYQLFSINTGYPVMYLVEDPKTFAVSRFSFPALLLTQNYISTTILVAMFAFIGQWKM
                     FILLNKLFPNFTKYAAIAVLFFPSPTFWSSGILKDTFIYGAMLWSIYNFYQIFVYKKN
                     IFGNLLLLLMNFYIIILIKPYVAIALLPTFLLLFSLSYIRKIKNRFVKYFITPLGLLF
                     AVILTIVIYTSIGSSLGPYGSIQSAIDKAYITQQDFIKNETYSAHKYDIGSFEPTISS
                     ILSKYPISIFYGLFGPFIWQADSFTMLLGALESLALFILTIYLLYKIRWRFFKFIFQD
                     DFIIFSFVFSLIFIFFVGLASANYGSLSRYRIPALPLFLASLFAIYNYCKMQKIF"
     gene            complement(12230..12613)
                     /locus_tag="PKI65_05605"
     CDS             complement(12230..12613)
                     /locus_tag="PKI65_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77951.1"
                     /translation="MLRHIGLNINSSEDVEYFYKNVLGFELIDEYYLHPSIVKTFLNC
                     EQVKIFRVKKFNLVLELILTDIKNPQSFSHIALEFWKPENIFEKAKNSGYAAYEFIKE
                     TGSKARYIIDNSGNIFEIKSINLIE"
     gene            complement(12728..15595)
                     /gene="gcvP"
                     /locus_tag="PKI65_05610"
     CDS             complement(12728..15595)
                     /gene="gcvP"
                     /locus_tag="PKI65_05610"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOB77952.1"
                     /translation="MLTHNFAYRHNGPNELEIKKMLETLGINSLDEMMEKTIPSDILR
                     KDLNIGIGKGLSETQVIELMKNFAFKNKIFKNYIGLGYYETALPGVIQRHILENPRFY
                     TSYTPYQAEISQGRLELLLAFQTMVAELTSMEVANASMLDEATAAAEAMTMSFNLRSR
                     DKVKNDARVFVVDERVFPQTLAIILTRAKYLDYDVIVTDVFKYNFTDDIFGILIQYPD
                     QYGEILDYTDVVNKAKNIDAKVVVAADIMSLALLTPPGEWGADIVIGSTQRMGLPMGF
                     GGPHAGYMATKLDYIRQMPGRIIGVSIDANGNKALRMTLQTREQHIKRERATSNICTA
                     QALLAMMAGAYAIYHGPDGIKEIAAHINQLTGLLAQEIQKIGYVQKNKYFFDTIRIEI
                     PTYVDMDTLKLKLLENNINVRYINNKEIGISLGEPTTMDDVNLLLSIFAKLTGKEPTT
                     FKFEDIKNIQIKTFDDKHKRNSIFMKQKIFKIYHSETAMMRYLTKLADRDLALDRTMI
                     PLGSCTMKLNASTELFHLSWPGFANLHPYAPKDQVQGYLELIDELGQLLIKITGMNGI
                     TFQPNSGAAGEYTGLITIRAYYEAKGQGYRNVCLIPASAHGTNPASAVTAGMKVVVVK
                     TDDKGNVDIEDLKTKAKENADKLAALMITYPSTHGVFEDNILEIINTIHQYGGQVYMD
                     GANMNAQVGFTSPGLMGADVCHLNLHKTFAIPHGGGGPGAGPIAVAKHLTPYLPSHPF
                     HPDGIITHSVNTVAAAPFGSAFILPISYIYIKMLGDDGLKAATQIAILNSNYLRKKLE
                     PYYKILYVGSNGFCAHEFIVDCNPFRAYGITELDIAKRLMDYSFHAPTVAFPVHGTLM
                     VEPTESEPLNELDRFVNAMISIKAEIDEVINEKIDLEKTTLKNAPHTLKKLLNDNWDL
                     PYTREKAAVNLKDKYWPPVARIDDAYGDRNIVCNWIEDLMD"
     gene            15752..16735
                     /locus_tag="PKI65_05615"
     CDS             15752..16735
                     /locus_tag="PKI65_05615"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOB77953.1"
                     /translation="MSNTFGKIIKVHLFGASHDNVVGVVIEGIPAGINIIEDDFIEYI
                     NRRKGGSVGTTDRVEADNPKILTGIIDEYTTGLPITIVFENKNKNSIPYQLYKSWHRP
                     GHADFTQKSKYGKYADIRGGGIASGRMTLPLMIAGVIASKCIPNVNAKAFIAHINGEK
                     PDKIDWQKIRNEGDSLGGEIICILENIIAGIGEPFFDNIESYFSHLFFSIPGIKAVGF
                     GDAWQTAFMRGSEYNDIFISNDGQTTTNHCGGIQGGITNGNAIVIHLIVRPPASIKKE
                     QISLNFDTDELEKRKITGSHDLAFILRLPPIIEAVSLLAIADLYLYSKIYS"
BASE COUNT         5852 a         2409 c         2567 g         5840 t
ORIGIN      
        1 gaaagacaat ttaaccttat tcactactga tgctaagacc tcgcgtaata gtgcacgtgc
       61 taatacagct gcgggagaat ctttattaga tgctgttaaa aaaacaaata atttacaaaa
      121 gaaaaaaaaa ttattagacg aagcaattta ttatctcaat agagctataa gtatttatcc
      181 tgaatactat gctgctcata atattttagg tgaagcttat ttcatgaaaa aaaattataa
      241 cgaggcactc aataatgcac taatatgtgc taagctaagg ccaatggaac tattaccata
      301 caaaaatatt tttgcagctg gcataaatgc ttattatgat tcatcttttg atgtagctaa
      361 tcatgctttt acatttctca ctaataattc ttattttata atgaaccagt tttcagactc
      421 tacatatttt actttacaat tagctttagc atttagatac ttgaataaaa ttgattctgc
      481 tattataata cttgaaaata tcataaagaa ggatacatgc tatgtagaag catttaatca
      541 attagctgaa ttgcatagta tgaaacctgg tgggttagaa acttctgaat attatttatt
      601 acgtggttat aattgtgatc ctaataatgc tgctatttta gagaatctag gcaccttaaa
      661 tgtgattaaa cataattatt ctcaagcact aatatatctt aaaaatgttt ataatattaa
      721 tccaaataat aaaaatgtta tcaaaaatct agctattgca tatcaaaatc taggaatgat
      781 agacagtgcc gagtattatg ccaataaatt acattgaaaa aagttgaatt gaaaaactat
      841 gatctatcac ctaatttaaa gatctaaact ccaaccattt gattaattgt cgaatgtgag
      901 atgtcaattt aatcagtata tgttatcaca attgaatcaa atatcaacca gaatacattg
      961 atatatgtta cattttcatt aattattaat ttagttttga ttatttgatt atttttatct
     1021 attttttcaa tattaatatt tgattttaaa tatatatttt taatatttat ttttttataa
     1081 acagattctt tagcagacca aaataaagta gtataaagtt taatatcatt tttaggtaaa
     1141 agtttttctt cttctatagt taaaaattta ttttttaatt ttaatgcttg attggatata
     1201 agttcgatgt ctagacctct attttcattt atattttcat tgatcaagat ggcagaataa
     1261 ccatctgtgt ggctaacact gatagctcga ggtggattta ctaaaaatgg cttattattt
     1321 tcatcataat aaattctgta aataaaatct gcttgcacaa tagaccttat aagagctcta
     1381 tagctcatcc attgtttttt tcgtttttca cttttaaatg attctaaggt atcgaattcc
     1441 tctttattta aatataaata tttaagcaac tcttctatgc tttcagtgat ttgccaaatt
     1501 cctaattcta atccttcttt tattttatta taaaaaatta ctggcatttt attgtattat
     1561 aagtttttgt ataaaaaaat tagttttatt attgataatg atcacgtata accctctatt
     1621 aaatgaaata ggtatattaa tatctgtagg attataagca atgtcattat aaattaattg
     1681 tccttggata gtatataatt ttatttgtac atcatcattt acgttaggaa aatagatatg
     1741 tataaaatca tcagtagtat aaatattagc aacaaaatta taatcaagta tatgtgcact
     1801 aggtccccaa atagaagcta cccattcggg atgatctata aagggattgc gattatgttg
     1861 tattacaaaa acagaatcat ttctttgctt ctctttggta cttactggat ctaactggtg
     1921 ccattgtaaa aataaatttt ttgcccaagg agctaaatct ccattttcaa agctttctcc
     1981 attccaatta ttaattttgt tcatatatct ggtagacata taaaaatata tacgagcgat
     2041 atcaccttta aaagaatcta taggctcaaa aacgataccg gaataccctg tagtaataga
     2101 actgcctact ttgctgccat taagagaggt ccagctagca cttcctacat tgccataagg
     2161 gtaattgctt cgtctattat ttacatagcc gtctgtaggc aatacatgaa aaagatcagt
     2221 atacattgga gaggcatcat taaaccagct tgcaggtaca gtatgctctc tattatagca
     2281 atcgccttca gagttataat taccgcattg atctatgata aatgtatatt catagggggg
     2341 aatacctcct ggaatatcac tgtaaatgtc ccatactttg ccgttaggtt ttttatctgt
     2401 tagtgggtat gcgttccata attggttata tgataatgta gttacattac tattgattat
     2461 attaaatagc gcttgcttga gttcttgacc acttttgccc tctgcattat catagtaacc
     2521 ataaggtatt tggctaaata aacagatagc gattaaacta aaaaataatg taatatttat
     2581 ttttttcata ttttttattt caacaaattt acaagatttt tttcatcagt taaaattatt
     2641 tttcgaaaat ggaaatttaa ttaaaaagaa atattattag aatgtgctaa agtataggaa
     2701 atcttttctt tataaaccta tttgagaagt gataatgttg tagattttat tatggatttt
     2761 gacaaatatt ttttttgata aataatatta aattttataa atttataaat ttttattaat
     2821 atgataaatg aataaatgat tcaaaaaatt acaataatta caggcggaca aagtggcgta
     2881 gatagagctg ctatggattt tgttttagat aatcctcaat taaaaataaa atgtggaggg
     2941 ttttgcccta aaggtagaaa agcagaagat ggtataatac ctcaaaaata tccgcttatt
     3001 gagtgtgaga ccgacaatta cgcagttcgt acagagttga atgtaaaaaa tagtgatgga
     3061 gttttgatta tttttaaggt aaaaattaat gatcccggta ctacattaac agtagaatta
     3121 gctaaaaaac ataataaacc attatatctg tgcaatatca aacaaaataa cattaatgaa
     3181 ataagaaaat ggattagtga aaatgcgata aatactttaa atattgcagg tccacgtctt
     3241 agcaatgacc ctgagatata tgaattgaca tatgattttt tagttcaatt atttcttgta
     3301 taaatttatg taattattaa aaatctatcc attgcccatc tattgcaaat tataatataa
     3361 tgagctacga agatttgttc ataaaatata ttgcccaaat aatttttgaa cttttcaata
     3421 ttatcaataa gtattattaa tgaatatatt tactaaatct ttgttttact tataattttg
     3481 tgcttaggtt ctgtatgaaa ataataattt atttatgtgt tattttttca aaatatgttc
     3541 aaaatggcaa aaaacaatga ttaaaataat tttttaaata aaaaaaactt cattaatttt
     3601 gtctgattat ttttaaataa aaaaatataa aaaatataaa aatgaaaagg aatcttttgt
     3661 taataagtaa ctctacaatg gcaggtgaag tatatttggg ctggtgcaaa gaattaatta
     3721 ctaattttct aaaaaaattt aatatttatg aagtactttt tatcccatat gctggggtaa
     3781 atttggactc taaaagtata gaatttagtt ataatgctta cgagagtaag gtgcaacgtg
     3841 tctttgatac agataatatt gatatcaata gtatacatca ttattccgat cccactaatg
     3901 cagtattaaa tgctaaatgt ataatggtag gcggcggtaa tacatttcat ttagtttacg
     3961 aattatacaa aaataatttg atgacaacta tacgtgaaaa atccttgtgt ggtattccat
     4021 atataggttg gagtgctggt gctaatcttg cctgtcctac tattaaaact actaacgata
     4081 tgccaattgt tttcccaaaa tcttttgatg ctcttaattt aatcgatttt cagataaatc
     4141 ctcattatat agatcataac cctgctgatc atggtggcga aactcgtgaa cagagaatat
     4201 tagagttttt agtagttaat aaagatatga aagtattggg cctacgagaa ggtacttact
     4261 tagaaattta taatgataat atgtggctaa aaggtgacaa acctgctcgt ctctttcaat
     4321 ttaatactga accaagagaa ataaaacctg gcgaaaatat gattattatt tgataaaaaa
     4381 accttaattt aaaataatat atatgaataa aaaaataaaa tttatagttt tatctattat
     4441 aattatattt tacaattcta ttaaagtgaa tgcccaatta cccatagatc ctgacactaa
     4501 aaaaattaaa tatcaagagg tagttcaaga gcagggaaac aaagatacac tttataatag
     4561 agctatcaga tggataaata gtttcttcga aaatcctcaa ggaattacta aagtgagaga
     4621 tcctgaaaat ggtaaaatcg taggtactta tcgtatgcga atgatagata ctttagttga
     4681 tggcagtaaa acattaagca ataccatagt gatttgtact tttactattg aagcaaagga
     4741 tgggagatat cgttacacac tagatgattt ctatgtacct gcagtatcta aaatgttcct
     4801 agaaaaatgg atggataaaa ccgatccata ttatttaccc aaatatgata gttatctaaa
     4861 gcaagtagat gaatatgtta atagatttat caaaagcctc aaagaaggaa tgaaaccgcc
     4921 tataattaaa atagacgaat ggtgagtatt gttttgtact catcttttat attcattttt
     4981 attgctgata gatgtttagc taattataat ttttttatgt tatgattcct ttttttacat
     5041 ttattttata aaaactaata cctaattcct ttttttttat tatttttgta ttaaaatagt
     5101 tgtttaataa ttaattataa aattatgaaa aaaatactag tatttttatt tttattagtt
     5161 cctattttgc ttcatagcca gttatatatt aatacatcgt atattccaca acaattggtc
     5221 gaagattttt tgataggacc aggcattact gtatcaaatg taactcatag agggcaattg
     5281 caacaatttg cttatttttc taatggtaat agcactgttt tgggtatgga tgaaggtata
     5341 atcctgtgta caggtactgc ttctaatata gctcaaaatg gaagcagctt tatgagtaat
     5401 agtcttggag gtcttggagt aacagagctt gattcaatag ttagtccata tacgactaat
     5461 gatggtgcgg tactagaatt tgattttata ccattagata ctttgctcac cttttatata
     5521 tttttggaag tgaagaatat ccagaatttg tatgttcttc ttttaatgat gtgtttgctt
     5581 ttctaatatc aggtatcaat cctagtggtg gaatgtatgt agacaaaaat attgcactag
     5641 tgcctggcac agatttgcct gtagctatca atacaataaa tccaggtgtc cctggatcag
     5701 gattttctgc ttcagagtgt attagtttat catattctaa tttgtatata aataataatg
     5761 attcatatca gcaaccagta tttgatggtt ttaccatacc actagtggct gaggcaaaag
     5821 taataccatg ccaaacatat catattaaat tatgcattgc tgacgttact gaccatgtat
     5881 atgattcagg tgttttctta aagcgtaatt cttttttaac taatctacct gaattaaaca
     5941 ttgttactcc ctatgctagc ccagatacta tttatgaagg atgtgcacct atagatttag
     6001 ttttttcaca tcccgaacca gtaccttatc atactaatat tgcaataggt ataggtggaa
     6061 cagctactaa tggagtcgat tatacttatt tatctaatat cattactata cctgctgact
     6121 ctacttctgt ctctcatact atttatgctt ttgctgatgg aatcgatgag gctgatgaat
     6181 atctcattat taatattagt ggttattgtg gtttgataag ctctgatacc atatggataa
     6241 aagataatcc tttttttgta acattaaatg atactacgat atgtgaagat gatgtcccat
     6301 taacattgac acctactata catgatggtc aggctcctta taattttatt tggaatactg
     6361 gggatgataa tgaaaatttg attatttatc ctgatttcac atctacctat tctgtcactg
     6421 ttagtgataa tttgagttgt tccgcgtctg ctgaagctct aataactgta aatccactcc
     6481 ccgatgtcta tattaacccc tccgatactt caatttgtaa aaatgagttt attacgctta
     6541 ctgtttatgg ttcagatttt tacgtttggt cagatggaag tacagatagt tctataactg
     6601 tatcaccaca gactacaact atatattctg tgacggggac agacagcagt ggatgttcta
     6661 atagtgcatt agctacagta aatataatag aaagtccttc gctaaatata actgcttcgc
     6721 ctcctaaatt atgttcaggc gaaagtagta aattaatagt aagtggtgca caaacttatc
     6781 tgtggagcac tggtgaaact acaagtcaga taatcgtttc acctcaaatt acaactagtt
     6841 atactgttac tggtagtatt caaaactgtt ctgatactaa aagcattaca atagaagtga
     6901 tcccaaatcc cgaagttaat ataacagctt cggctactca tttacttgct ggagatatag
     6961 catccttgca agcagaaggt gctaatgaat ttttatggta tggagacaat tctattagct
     7021 gtataaattg tcctactatt gaagtatcac cactctcttc tactgagatt tgcgttatag
     7081 gttttaatag tatttgtact gacactgctt gtgtatttat agaagtagac ccttgtacat
     7141 tatttatacc taatgccttt actcctatta aaaatagctt gaatgatgta tggcaaattt
     7201 ttactccttg tcctattgat aaaggggaaa taagaatata taatcgttgg ggaaaacaaa
     7261 tattttatac tacagacctt tattctagtt gggatggaac cttcgaaggt gaattagtgc
     7321 cccctggtgt ttattctttt atgattcgtt atgcttatgc tcatacaaac tctatatatt
     7381 atacccgcaa aggtactatt actattcttt attaattttt tatataatca cgaattctat
     7441 tatatacatt aaaaatatgt tttaataaaa aattgaaatt aatttacaat tttataaaaa
     7501 taaactgtaa attatcaaaa acaattaaat aattaacaat aaaaataacc caaaatagtg
     7561 attatataat tgaaccattc atttagagtt tcattcctat ggtaaatgaa actaatatat
     7621 tattcataga tagattagct ttaggctcag gtttgttgag tatttccatt actagaggat
     7681 catataaata tctttgatca ttatgtaaat aataatttat accaacatca aaaattaata
     7741 tattttctct gtatccaaat cctagaccta taacatgtgt atcgaaatta ctaatatttt
     7801 tatatggtga tgaataataa gcatatccag ctctcatggc aaatggcttc aatacccatt
     7861 cagtacctac tcttatatta tgtccataag ttaattcatt attaatacta ttatttaggt
     7921 caaaaaaagt agtatttgct ttttttgtac taaaatgatt ttgcgtataa tctgtccact
     7981 cgtattctgc atttataaat ccattttcac caaatataaa ccccaaccca ataatagcac
     8041 gcataggatt ataatatcta taattaaatt ctcctcgagg actatctgca gtatagatac
     8101 cattgtatac atttgttcgc atggttctaa aatacgaatc atgtaaccag aaataagtag
     8161 gactatgaaa agctagcgaa aatctaaacc attgtattgg tttatacata aaacctattt
     8221 tgaaatttaa gccttcacca tctgtattta aataatcaaa aacttctaaa gatttgaaat
     8281 caggtatggt atctttaaca tcttcttcgc tataagtaga ttctctttca aaagaaaaaa
     8341 ttgggaaatt aattgatcca ccaatatata atttatcatc gtaattacct gctattgcca
     8401 aatttatttc atacatactg ccactattaa tttctaaata gtcttgccta acacctcggg
     8461 tgactattcc ctcataggca gtaggtgtgc ccggtattgt atctaaaagc cacgtatcga
     8521 aagccaacca tgtatcaaaa acatttaatt gattaggtaa aaaaccatta gcatagacag
     8581 aataaacatc tacaatacta ctataagggt tgtctgccga aatcattatc ctgctattaa
     8641 aatcagctaa cttagaatag ctaagaccaa aattcaaatt tttccaatcg tgtttgtctg
     8701 tattaaaatt agatgaaata actaatccca tgctaggtag tgaaaccaca gttttaatat
     8761 catcattaga attatttaag tatttagaat tagataaaaa attagagata gaaaaagatc
     8821 cagatatttc tgatttttta taaacaccaa gtccggcagg attgaggcta ataccagtaa
     8881 tattagctcc aataccagag aatgctccag atacggcatt aaatctgcta tctccataag
     8941 gcattagata tgaatatctg actgcatcat cgaggttttg tgcatatata cctgtatatc
     9001 ctataattac taggcataaa cctaaataaa aacttaattt tttcatatta atatattttt
     9061 taaattaata ttttatcttc taccaccacg agatggtgca gaaccggaac ccgaacttct
     9121 tggagtatta ctatgagagc taccagatga actagatcta gatggggcat tataagaagg
     9181 agaactatta ttactacgat taggactact ataattatta gcaggggggc ttgaatatct
     9241 agttggctgt actggttggt ttttattagg agatggtcta gaataactac ggcttggagt
     9301 ttgtgtttgc gaaggtgtaa ctttgctagg attagattct tgtggttttt gatattgatt
     9361 agtgttttgt tgctgagtag gctgagttat tggtttttgt tgctgggaag gttgttttat
     9421 attagctggt tgagtaggct gagttattgg tttttgttgc tgggaaggtt gttttatatt
     9481 agctggttga gtaggctgag ttattggttt ttgttgctgg gaaggttgtt ttatattagc
     9541 tggttgagta ggttgagtta ttggtttttg ttgctgagta ggttgtttta tattagctgg
     9601 ttgagtaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng agttattggt
     9721 ttttgttgct gagtaggttg ttttatatta gctggttgag taggctgagt tattggtttt
     9781 tgttgctgag taggttgttt tatattagct ggttgagtag gttgagttat tggtttttgt
     9841 tcattaatag gtttatctat tatagcgggt tttataggtt gttcacttgt agttacaggt
     9901 ttttgtatag gttttttatc agtatctaaa ttattaggca atttgtcgtt tagtgtagca
     9961 ttttggtcta tatttatcaa agaagaaggt ttagtaatag ttatctcttg gggcttgact
    10021 tctaatgggg tgtaaccaac tctactcaat ctatcagcaa aagtcaaatt agaagcaggg
    10081 gttctattaa gcgatgcagg aggtatatta tcattgatag ttctattttg cctgccacta
    10141 gtagatgcac ttatataatc tcgaggtcca tagtaaacgg ttccagagtg cacatcaaaa
    10201 ggattatagt aaaaatattc tgacccataa aatccatgat aatatccaag ccaaaatccg
    10261 tcccaatatc cattatgata gccatgccag tagccataag gataacccca gctataataa
    10321 ggataacacc atcctaaata atagttgtat gggtgataac cccagtaaaa ggaaaatcca
    10381 ggtctccaaa aaggatatgt aagatatata gaaataccaa aataccaagg atcataagta
    10441 taccaataca tattagtata ccatggagca taatatccaa aacctaattc aggataataa
    10501 aaacgacgta tacgagatga ataagaaaaa tcataataat cgccataata attgtttgtt
    10561 atgtaagtgt tgccatacga atcagtatat gtttcagaat aataaggctc agcttgagta
    10621 ggatcatatt gctgattttc atatgattga taattttgat catttggtgc tttagcactt
    10681 gaatactggt gtacttgtgg attgtaatat acatcgtcat aaggtgtatt atcagtgtag
    10741 tagttattta tagtacatcc actaaaaatc aaccccacca cacaggctat taaaaaattt
    10801 aaggttttca taactcctcc ttctttttta taatatattg caattattat accaaaatat
    10861 aagaaaaata ataaaactaa aaaatctttt gcattttgca ataattatat atagcaaaaa
    10921 ggctagctaa aaacaatggt agtgcaggta tacgatatct gctaagagat ccataattag
    10981 cagaagcaag cccaacaaaa aagataaaaa ttaatgaaaa aacaaaagaa aaaataataa
    11041 aatcatcttg aaaaataaat ttaaaaaatc gccatctaat tttgtataag agataaatag
    11101 tcaatataaa taaagccaaa ctttctaatg cacctaaaag catagtaaag ctatcagctt
    11161 gccaaataaa aggcccgaag agtccataaa aaatagaaat aggatattta gataaaatac
    11221 tagaaatcgt aggttcgaaa gaaccgatat catatttatg agcgctataa gtttcatttt
    11281 ttataaaatc ttgttgagta atgtatgctt tatcgattgc actttgtata cttccataag
    11341 gtcctaaaga agatccaata gaggtgtaaa taacaatagt taaaataaca gcaaacaaaa
    11401 gccccaaagg tgtaataaaa tatttaacaa aacgattttt tatttttcta atataagaaa
    11461 gtgaaaataa aagtaaaaat gtaggtaaaa gagctatagc tacgtaaggt tttattaaaa
    11521 ttataatata aaaattcatt aataataata atagattacc aaaaatattt tttttataaa
    11581 caaaaatttg ataaaaatta tatatgctcc atagcatagc tccgtaaata aaagtatctt
    11641 ttagtatacc actactccaa aaagtaggtg atggaaaaaa caatactgct atagcagcgt
    11701 atttagtaaa atttggaaat aacttattaa gcaaaataaa cattttccat tgcccgataa
    11761 aagcaaacat agcaactaaa attgtagtag aaatataatt ttgtgttaaa agcaatgctg
    11821 gaaaagaaaa ccgagaaact gcgaaagttt ttgggtcttc tactaaatac ataacgggat
    11881 agccagtatt aattgaaaac aattgataaa aatcattata attcatattt cctgtaaaaa
    11941 taattttgct tattttgcca ggatgattaa aaaaagcatt tacaaagcat ttagtattat
    12001 agcaatacca ataagcatca cctccaccat aataaaaata ataaataaat ataaaagcaa
    12061 tgctcataag tattttgaaa ccaagaccat acattaaata cttatgaaaa atattatcag
    12121 taacaacttt gctgtaaaaa tatattaaag aaaaaatagt aataattaaa atcagagaaa
    12181 taagaatatt ttcaaaagaa aatataggat ctaagcccat tataaataat tattctataa
    12241 ggttaatact tttaatctca aaaatattac cagagttatc aatgatatac cttgctttag
    12301 agccagtttc tttaataaat tcataagctg catacccaga gttctttgct ttttcaaaaa
    12361 tattttctgg tttccaaaat tctaaagcta tatgagaaaa agattgagga tttttgatat
    12421 cagttaaaat taattccaat actaaattaa attttttaac tctaaaaatt ttaacttgtt
    12481 cacagtttaa aaaagttttt actattgatg ggtgcaaata atattcatct ataagctcaa
    12541 atccgagaac atttttataa aaatattcaa catcttcaga tgaattgatg tttaaaccta
    12601 tatgtctaag cattattaat tttatcgcaa atttacatat aatttttgta ttgtgattaa
    12661 tagatttttt tatttttatt gaagttttgt atattagtat ctaaatgaag ataatctcaa
    12721 caatacttca atccatcaaa tcttctatcc aattacaaac aatatttcta tcaccataag
    12781 cgtcatctat acgagctaca ggtggccaat atttatcttt aagattaaca gctgcttttt
    12841 ctctagtata aggcaaatcc caattatcat taagcagttt ttttaaagta tggggagcat
    12901 tttttaaagt agttttttct aaatcaattt tttcatttat tacttcatct atttcagctt
    12961 taatagatat catagcattt acaaatctat caagttcatt gaggggttcg ctttctgtag
    13021 gctctaccat cagtgtacca tgaactggaa aggcaactgt aggagcatga aaactataat
    13081 ccatcaacct tttagctata tctaattcag ttattccata agcacgaaaa ggattacaat
    13141 caactataaa ctcatgagca caaaaaccat tggagccaac atatagaatt ttgtaataag
    13201 gttcaagttt ttttcttaaa taattagaat tgaggatggc aatctgtgtg gcagctttta
    13261 aaccatcatc acctaacatt ttaatataaa tataagaaat aggaagaata aacgcactac
    13321 caaagggagc tgcagccaca gtatttacag aatgagtgat aatgccatca ggatgaaatg
    13381 gatgagaagg cagataaggt gttaagtgtt tagcaacagc aataggacct gcacctggtc
    13441 caccaccgcc atgaggtata gcaaaagttt tgtgtaaatt gagatgacat acatcagcac
    13501 ccattaagcc aggagaagtg aaacctacct gagcattcat attagcacca tccatatata
    13561 cttgtccacc atactgatgt atggtattta taatttctaa aatattatct tcaaaaacac
    13621 cgtgagtact gggataggta atcataagag cagctagttt atcagcattt tcttttgctt
    13681 tagtttttag atcttctata tccacgttgc ctttatcatc agttttcact actactacct
    13741 tcataccagc tgttacagca ctagcaggat tggtcccatg ggcagatgca ggtatcaagc
    13801 atacatttct atatccttgc cctttagctt cgtaataagc tctgatggtt attagtccag
    13861 tatattcacc agcagcacca ctatttggtt gaaaagtaat accattcata ccagttattt
    13921 taataagtaa ttgtcctaat tcatctatca attctaagta accttgtact tgatctttag
    13981 gtgcatacgg atgtagatta gcaaagcctg gccaagagag atgaaatagt tcagtagatg
    14041 cattgagttt catggtacag ctacccagag gtatcatggt cctatctaga gccaaatccc
    14101 tatcagctaa tttagtcaaa tatctcatca ttgctgtctc actatgatat atcttaaaaa
    14161 tcttttgctt cataaaaata gagttacgtt tgtgtttatc atcaaaagtt ttaatttgta
    14221 tattttttat atcttcaaat ttgaatgtgg taggttcttt gccagtaagt ttagcaaaaa
    14281 tgcttagcaa aagatttaca tcatccatag tagtaggttc accaagactg atacctattt
    14341 ctttattatt aatatatctt acatttatat tgttttctaa tagtttcagt ttgagagtat
    14401 ccatatcaac gtaagtggga atttcaattc taatagtgtc aaagaagtat ttattttttt
    14461 gaacatatcc aattttttga atttcttgag ctaataaacc tgtcagttga ttgatatgag
    14521 ctgctatttc cttaatgcca tcgggtccat gatagatagc ataagcacca gccatcatag
    14581 caagtagcgc ctgagctgta caaatattag atgttgctcg ttctcgctta atatgttgct
    14641 cgcgagtttg tagagtcata cgcaaagcct tattaccatt agcatctatt gatactccaa
    14701 taattctacc tggcatttga cggatataat caagtttagt agccatgtaa cctgcatgag
    14761 gtccaccaaa gcccattggt aatcccattc tctgagtact acctatcaca atatctgcgc
    14821 cccattcacc tggaggtgta agtaatgcta aggacataat atctgctgca actacaactt
    14881 tagcatctat atttttagct ttgttcacta catcagtgta atctagtatt tctccatact
    14941 gatcaggata ctgaattaaa ataccaaata tatcatctgt aaaattatat ttaaaaacat
    15001 ctgtgacaat tacatcataa tctaaatatt tagctctagt gagtatgata gcaagtgtct
    15061 gaggaaatac tcgctcatct acaacaaaaa cacgagcatc attttttact ttatcacgac
    15121 tgcgaagatt aaaagacata gtcatagctt ctgcagcagc agtagcttcg tctagcatag
    15181 aagcattagc tacctccata ctagtaagtt ctgcaaccat tgtttgaaaa gctagtaata
    15241 attctagtct gccttggcta atttcagctt gatatggcgt gtatgaagta taaaatctag
    15301 gattttcgag aatatgcctt tgtattacgc ctggtagggc agtctcataa tatcccaacc
    15361 caatataatt tttaaaaatt ttatttttaa aagcaaaatt tttcatcaat tctatcactt
    15421 gtgtctcact aagaccttta cctataccta tgtttagatc ttttcgtagg atatcagatg
    15481 ggatagtttt ttccatcatt tcatctaacg agttgatacc taaggtctct aacatttttt
    15541 tgatttctag ttcattaggg ccattatgcc gatatgcaaa attatgagta agcataaatt
    15601 aaatttttta ggattaataa gtgcaaaaat aactaaaaat atgaaaataa tatcaaaaaa
    15661 tattattaat aataaatgta gttttataat aaacttttca aaaattttat tttaattttg
    15721 taattacatt gataaattaa atgtcaaaaa aatgtcaaac acttttggga aaattataaa
    15781 agttcattta ttcggtgctt cgcatgataa tgtagttggt gtagtaatcg aaggaattcc
    15841 tgctggaata aatattattg aagatgattt tatagaatat ataaatcgta gaaaaggtgg
    15901 tagtgtaggt acaactgata gagtagaagc agataatcca aaaatattaa ctggtataat
    15961 agatgaatat actactggtt tgcccattac aattgtattc gaaaataaaa ataaaaattc
    16021 aattccctat caactatata aatcttggca tagacctgga catgcagatt ttactcaaaa
    16081 atctaaatat ggaaaatatg ccgatattag aggtgggggt atagcatcag gtaggatgac
    16141 tttaccatta atgatcgcag gggttatagc atctaaatgt atacctaatg taaatgctaa
    16201 agcctttatt gcacatatta atggtgaaaa acctgataaa atagattggc aaaagattcg
    16261 aaatgaaggg gatagtcttg gtggagagat aatatgcata ttagaaaata ttattgctgg
    16321 tataggcgaa cctttttttg ataatataga aagctatttt agtcatctat ttttttctat
    16381 tcctgggatt aaagctgtag gtttcggaga tgcatggcaa actgccttta tgagaggtag
    16441 tgagtataat gatattttta ttagcaatga tgggcagacg accactaatc attgtggagg
    16501 tattcaaggt gggataacca atggtaatgc aatagtaata catttaattg tacgaccacc
    16561 tgcaagtata aagaaagaac aaataagctt aaattttgat acagacgaat tagagaaacg
    16621 aaagataacg gggtcccatg atttagcctt tatacttaga ttgccaccta tcatagaagc
    16681 ggtgtcttta ttagcaatag cagatttgta tttatattct aaaatatatt cataaatgat
    16741 attttgttga atgaaattaa taaatgtt
//
LOCUS       DAOPRN010000040        16642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_2926_length_16642_cov_110.663833, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000040 DAOPRN010000000
VERSION     DAOPRN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16642
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2926_length_16642_cov_110.663833"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PKI65_05620"
     CDS             <1..459
                     /locus_tag="PKI65_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB77954.1"
                     /translation="TMAAMAGNYFTAASESTFDTRSWFLTPSFNNDTLSSYAPLSIRD
                     PYNYSNPDFRLLSNSPLLSSSSFVSIGENAISNLVVNIFPNPSIDYISIDSDNNIESL
                     EIIDILGQIYYSEYINANRITINISNLPFGNYIIRMISNNQLIQKLFIKL"
     gene            579..1247
                     /locus_tag="PKI65_05625"
     CDS             579..1247
                     /locus_tag="PKI65_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOB77955.1"
                     /translation="MQILIVDDEKDIIEFLSYNLTKDGYKVLKASSGMKALEIVDKHK
                     PEIIVLDIMMPEMDGIETCRQIRELIKNYHPYIIFLTARNEDFTQIAALEAGGDDFIA
                     KPIKPRVFISKIHSIERRLNNDKELTNEITIRNITINPERFEVIKDDMPNILPRKEFE
                     LLYELAKRPGRVYTRDELLDKVWGNDIIVGERTIDVHISKLREKLGADLIQTIKGVGY
                     KINP"
     gene            1248..2309
                     /locus_tag="PKI65_05630"
     CDS             1248..2309
                     /locus_tag="PKI65_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOB77956.1"
                     /translation="MFKKFLNAGDPKNLSLVFTAIVSLLLIINFILFISIDLHVFIWI
                     IILLIEIICIYFLFKKINVKFIYDKIRLIYKNIATKRLGDDKQPIQEWVKGDVLNRVQ
                     QDVERWLELKNLEIATLKELENFRKDFLANVSHELKTPLTTLQGYVETLIDGAIADPE
                     VNLKYLQRSSFTIERMISMVEDLETITKLEQNSDHLKIEKFDFISLVKEVLDMMELKA
                     NTRNIKMIIQNHTTIPVYVLADVEKIRQVLVNLIDNSIKYGKEFGNTFIKIYYFEQNL
                     LIEVEDNGIGIPEEHLQRIFERFYRVDKSRSREVGGTGLGLAIVKHIIEAHGQHINLR
                     SKVGIGTTFTFTLPLASTK"
     gene            2375..2920
                     /locus_tag="PKI65_05635"
     CDS             2375..2920
                     /locus_tag="PKI65_05635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOB77957.1"
                     /translation="MNFSRILILLFIIFSFQSCECNRKNKDNSSIDNIDVRQYEESLL
                     EANKLYLEQEHRDISAYIHRKGWQMDTTPRGVYYMIYYRGDGYIPERGDIVRFAFKTM
                     LINDQVCYDSEIDGPKEVELGKADIESGLEEVLYYLPEGTKAYVIIPSHLAFGWLGDV
                     ERIPSRAVLIYDLHILKVIKI"
     gene            complement(2926..3561)
                     /locus_tag="PKI65_05640"
     CDS             complement(2926..3561)
                     /locus_tag="PKI65_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77958.1"
                     /translation="MKLKIFITLFIIILPTVYYAQDDIVIRKYLFNDGVYVKPQSLYY
                     NTPDYTDFYILKNKYGDILDIEVPCKDDSLKRYCSIDTFFAFVFENSLYIKQAYENSF
                     YRATIIGALVHFFDIEISYYHSYDDFFYYNYWVSYPVTERRKVNVEYVVELETGLRFY
                     FNYNNFLNFLKERDEALYNELKNAKKKQKIIYNFLLRYNSRHPLPVPYEEF"
     gene            complement(3573..3953)
                     /locus_tag="PKI65_05645"
     CDS             complement(3573..3953)
                     /locus_tag="PKI65_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOB77959.1"
                     /translation="MELEIGLTHEAFKLVGQDDSAIQYDSGLVDVFSTPALVSFMEKT
                     CMDCVDPLLDPDYCTVGIEIHVKHIKATKIGDTVRSIAKLIKIEGNVLTFEVKAYDSY
                     GLIGEGIYKRYIIHKPTFLQKLDQ"
     gene            complement(3972..4045)
                     /locus_tag="PKI65_05650"
     tRNA            complement(3972..4045)
                     /locus_tag="PKI65_05650"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4009..4011),aa:Arg,seq:tcg)
     gene            4143..5009
                     /locus_tag="PKI65_05655"
     CDS             4143..5009
                     /locus_tag="PKI65_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOB77960.1"
                     /translation="MYQLVIQKRITIRENEVLAEYLDEVSQEPLLTPEEEVELAKRIK
                     MGDKEARNKLIKANLRFVISVAKQFSQTGLPLTDLINEGNIGLIKAAEKFDETKGFKF
                     ISYAVWWIRQGIIQALSEHGRIIRLPGNKLTLINKVKNAYNLLEQINSRPPTIDELSD
                     MTKLSPDQVKELLNYVDNNVDSLDNTISEESENTIIDFIPNHNSPAPDTNLEQESLKI
                     EIQRILQILPIRERQIIKYYYGLDGTHALSIEEIAKRMDLSTERVRQIRDKALRKLKN
                     NKEIKLLNQYIN"
     gene            complement(5034..6491)
                     /locus_tag="PKI65_05660"
     CDS             complement(5034..6491)
                     /locus_tag="PKI65_05660"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOB77961.1"
                     /translation="MLDKSKKTIPNKTIFWIFLSAIILAVVNTIAMGYEFYYVALIPF
                     LLLLLYYYFFSYEKIFLALAVIIPFSITIENIDFNLALSIPSEPILLGLLILALYRFI
                     SNNSPIGYSKHSITITIIIYFVWLVFSAFFSQIPVVSWKFVLAHAWLMIPAYFLGVSV
                     FFKDIKYQNIFLNLYLFSLVIIVIIAVIKHAGYNFDFKMSYLVSQPFYRDHTIYGAAL
                     SLIIPFAFMQTFNKTNHLNNRILYFICFLVLTLGLILSSSRAAWVSTAIALVVGVIIL
                     LGIKFRYVLLGLVIIFTLFFINYQSIMFQMERNPNVSRKDATSHVRSITNISTDLSNT
                     ERINRWVSAIRIAQDHPVVGSGPGTYQFIYAPYQLPQYQTYISTNTGTKGNAHSEFLG
                     PLSETGIPGMLTVIAIVISLFVTGFRLFFKLKKLDTNRSMIVLSVTIALLAYFLHGLL
                     NNFLDTDKIAYPVWAYAAILTANYYWLHWYKKEMN"
     gene            complement(6481..7521)
                     /locus_tag="PKI65_05665"
     CDS             complement(6481..7521)
                     /locus_tag="PKI65_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOB77962.1"
                     /translation="MEDFKPSIDEQVNFDSSNFFIFFIKWRKILIITTLVAAIIAAII
                     SLLIKEKYEAKVTLFPTMSNAISKALITDQGGREDILQFGEEEQAQQMIQILKSNEIR
                     DTIIKKFDLANHWKIDPKYKYARTALYKEYESNIKFRRTEYLSVEIKVRDEDPILAAN
                     IANTIAELYDSTKLKIQRQRALEGFKIVEGTYLEIQQQINQKEDSLKWLNQKGIGDYN
                     LQVERLTEAIGREIGRGNAHAVNILQAQLDTITKYGALYKQINEQLALLNRRLIEFQQ
                     KYDEAKIDAFSKIPQKFVVEYAYPPDRKAYPVRWLIVVVSALAAFVLTLVCLIFYENY
                     EKYKKKLKYSAR"
     gene            complement(7719..7934)
                     /locus_tag="PKI65_05670"
     CDS             complement(7719..7934)
                     /locus_tag="PKI65_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOB77963.1"
                     /translation="MYWTLELASKLEDAPWPATKEELLDYAYRTGAPPELIQNLLEIE
                     DEEKEFEGIDDLWPDYPDFKEFFDEEE"
     gene            complement(7992..8408)
                     /locus_tag="PKI65_05675"
     CDS             complement(7992..8408)
                     /locus_tag="PKI65_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77964.1"
                     /translation="MRMFKNKYTLFLLIFTFFITTACVNDNEDEDLREYFAGTWKCKE
                     INTGFAYNVVIKIDSTTLNYINIYNFHQFGNNEIVRAIVNDLAVDIPSQLTCNNSINV
                     RGSGLMQNNKTTINFDYIVNDGNTIDTINAVYTKIY"
     gene            8527..10317
                     /gene="lepA"
                     /locus_tag="PKI65_05680"
     CDS             8527..10317
                     /gene="lepA"
                     /locus_tag="PKI65_05680"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOB77965.1"
                     /translation="MNKLKRNFCIIAHIDHGKSTLADRLLEHTHTIDQRKMMDQVLDN
                     MDLERERGITIKSHAIRMQYNYKGQNYSLNLIDTPGHVDFSYEVSRAIASCEGALLVI
                     DATQGIQAQTISNLYQALDHNLEIIPIINKIDMPNADPEVVKDQIIDLIGCSRDDIIE
                     VSAKLGIGINKVLEAIIEKIPPPTGDYDLPLEALIFDSIFNPYRGVIAYFRIFNGTLK
                     TNDKIMFYSNKTIYEAEEIGYLGFYNEPCNEMKAGDVGYIITGIKSTKDIRVGDTITL
                     ADNPCNKAIEGFKPVKPMVYAGVYPVSPDDYDNLRESLERLQLNDPALEFTLETSNAL
                     GYGFRCGFLGLLHMEIVQERLEREFNIDVIITVPNVSYKIYTTKGEIVEIHNPVGLPD
                     PSKIDRIEEPYIKAQIITKSEFIGNIIKLCIDRRGVFVGQVYLSSDRVELTFEMPLVE
                     ILFDFYDKLKSISRGYASFDYFDIGYRETDLVKLDILLNGEKVDALSILIHRNNAYSF
                     GRRMCEKLKNLIPRHQFDIAIQAAIGGKIIARETIKAYRKDVTAKCYGGDITRKRKLI
                     EKQKEGKKRMRQIGKIDVPQSAFLSIFKLD"
     gene            10441..11010
                     /locus_tag="PKI65_05685"
     CDS             10441..11010
                     /locus_tag="PKI65_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOB77966.1"
                     /translation="MATRIRLQRRGKKHQPYYTIVVADSSAPRDGRFIEKLGYYDPMT
                     DPATIEIDIDRALYWLQVGAKPSETARSLLSNKGVMLKFHLWKGIQKGALTEEEAEQK
                     FQQWLKEKEERVNKAKESKRQKEKELYQRKLEQEKQVRIKKEEILAAKQAEKTEKEAQ
                     TEAQTENHTEEPAEVPTEEKTDLEEENTQ"
     gene            11027..11566
                     /locus_tag="PKI65_05690"
     CDS             11027..11566
                     /locus_tag="PKI65_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77967.1"
                     /translation="MIFPNKNSLISIGKIVKSFGINGHLLVNSEFLDDYNFNKRIAIF
                     VYWEYCFVPLIIEDLENRGDNYYLVKFELIDNVDDASKITGCNIYAFKDDIEIEKIDY
                     LSSPERFVGFDVIDISYGLLGKVIGILRGASPQLEVSSHEDSLFIQLIPEYMDHVDWD
                     NEIIFVNLPEQLFSFYGLK"
     gene            11553..12275
                     /locus_tag="PKI65_05695"
     CDS             11553..12275
                     /locus_tag="PKI65_05695"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOB77968.1"
                     /translation="MASSNPFYFKKFKVYDDKSTMRVNTDGVLLGALVTPSNDKNILE
                     IGCGAGYISMMLAQKNPYSLIHAIDIDSDTVAQANYNFRLCKWANRLAAYNIALEDYS
                     NISHQKYNLIVSNPPFHLEGMKSINTKKSLAKHTNIEGINNFFAAISNLLVKSGELWI
                     IISAKSYKTIISIAREHYLFPNKQINIKSIKTKSNSKVVVSFRKQITYPFDNLELVIT
                     NENQIYTDEFIELMKDYLLLKK"
     gene            12511..13812
                     /locus_tag="PKI65_05700"
     CDS             12511..13812
                     /locus_tag="PKI65_05700"
                     /inference="COORDINATES: protein motif:HMM:NF028473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77969.1"
                     /translation="MKIIFRFFFVLLIFINGLNAQNINYNCFTIGDNGNDEAKTAIIT
                     SDGGYLIGGWTNSYNTDDYDIYLIKLSGKNNIEWSITIGGTEHEAANSIKQTNDGGYV
                     IAGYTNSYGFGSFDVYIVKIDKNGNIEWTRVIGGDKDDRANDIAQTDDGGYLIAGTTY
                     SYGGGYSDIYIIKLNNLGEIMWEKTIGGKGYDGANIIINSNEGGYLVGGYTSSYGSGG
                     EDIYLIKISNLGDLEWTRTIGGNGDENAYDIIQNDDNDYILVGHTTSYIEYEDDFYIV
                     KMDDNGNVKWTKSIGGIEHENAISILEVNDGGFIIVGSTFTYGAGLHDFYVTKINSYA
                     EVEWIKCFGTDKNDKAATIIKTNYDNFLIIGNTETKNYDKDILIIQIDKNGNGCCDSE
                     SGGEIKVGGVGSGGTIKDVISQTTTGGNISNVNSSKKEICQ"
     gene            complement(13837..14811)
                     /gene="pfkA"
                     /locus_tag="PKI65_05705"
     CDS             complement(13837..14811)
                     /gene="pfkA"
                     /locus_tag="PKI65_05705"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOB77970.1"
                     /translation="MKNIGVLTSGGDAPGMNAAIRAIVRTSIYNGRDIYGIYEGYHGL
                     IEGDIFKMQASDVSNIIQRGGTILRSARSQKFLEKKHRQKAYKNLCSKDIGSLIVIGG
                     DGTFKGACEFKNEYPDINIIGIPSTIDNDLYGTDYTIGFDTACNTVVEAVDKIRDTAS
                     SHSRIFFVEVMGRDAGYIALYTGIANGAEEILIPETKTNIDDLVDNIKNRWRINKKSS
                     IIIVAEGEETGGAVKVSQIVKEKLPDYEIRYTILGHIQRGGNPTMRDRLNASRMGYHA
                     VKSLIIGEDKIMIGIMNDQIVKIPLERAVKNFKAVDKDLIEMMYILAI"
     gene            14993..15808
                     /gene="panB"
                     /locus_tag="PKI65_05710"
     CDS             14993..15808
                     /gene="panB"
                     /locus_tag="PKI65_05710"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOB77971.1"
                     /translation="MKDSRDLPRITTYVLKEMKMRGEKISMLTAYDYSFAKILDKAGI
                     DVILVGDSAANVMAGYSTTLPITLEQMIYHGASVVKGVKRSLVVVDMPFGSYQGNSTL
                     ALESAIKIMKETGAQAVKLEGGREILEAIDRILSAGIPVMGHLGLTPQSINKFGTYTV
                     RATEKEEAEKLREDAMLLERAGCFGIVLEKIPALLAKEVAESSSVPIIGIGAGRWVDG
                     QVLVLHDMLGINQDFSPRFLRRYHNLEAEILKAVNQYIDDVKNLDFPNEYEQY"
     gene            15789..16526
                     /locus_tag="PKI65_05715"
     CDS             15789..16526
                     /locus_tag="PKI65_05715"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOB77972.1"
                     /translation="MNMNNINDWKVPIPILYEDNHLIAVHKPHGLLIQGDKSGDITLI
                     DLVKTYLKEKYQKPGEAFLGLIHRIDRPTGGIVIFAKTSKALTKMNKIFHDRQVKKIY
                     LAIVENKPTIDNGLLVHYLRKNEKNNKSYVSDIHKNNYKKAELYLNYITSSSKYHLLG
                     IELLTGRHHQIRAQLSHIACPIRGDLKYGARRSLPNGNIDLLSYYLEFIHPITGNKIS
                     ITTKIPEYNPWQFFKIPAKKWLDKVYF"
BASE COUNT         6207 a         2205 c         2600 g         5630 t
ORIGIN      
        1 actatggctg ctatggctgg aaactatttc actgctgcta gtgaatcaac ttttgataca
       61 cgctcttggt ttttaactcc ttcttttaat aacgatacac ttagttcata tgctccactt
      121 tcaataagag atccttacaa ctattctaat ccagatttta ggctgctaag taatagtcca
      181 ttattaagta gttctagctt tgtgagtata ggtgaaaatg caatatcaaa tttggttgtt
      241 aatatttttc caaatccatc aattgattat ataagtatag attctgataa taatattgaa
      301 tcgcttgaga ttattgatat tttaggccaa atttattata gtgaatatat taatgctaat
      361 cgcataacta ttaatatctc taatttacct tttggaaatt atataataag aatgatttct
      421 aacaatcaat taattcaaaa attatttata aaattatgaa taagctatta taatttttta
      481 tattattaaa aggcaattta attattgaag catacgaaaa taagttttta tttgttaaaa
      541 tgtctaattt tattaatttt gagaaaaaaa attaatttat gcaaatttta atagtcgatg
      601 atgaaaaaga tataattgaa tttttgagtt ataatcttac aaaagatgga tataaagttt
      661 taaaagctag tagtggtatg aaagcactcg aaattgtaga caagcataag ccagaaatta
      721 tagttctaga tattatgatg ccagagatgg atggtataga gacttgtaga caaatacgag
      781 aactaataaa aaattatcat ccttatatta tttttttaac agcacgtaat gaagatttta
      841 ctcaaatagc agctttagaa gctggtggag atgatttcat agctaaaccg attaaacctc
      901 gtgtttttat tagcaaaatt cattctatcg aacgtaggct aaataatgac aaggaattaa
      961 caaatgaaat tactattaga aatattacca tcaatccaga aagatttgaa gtaataaaag
     1021 atgatatgcc aaacattttg ccaagaaaag aatttgaatt actatatgaa ttagctaagc
     1081 gtccaggtag agtttatact cgtgatgaac tattggataa ggtgtggggt aatgatataa
     1141 tagtaggaga gcgtaccata gatgtacata tcagtaagct aagagaaaaa ttaggtgcag
     1201 accttataca gactatcaaa ggtgttggtt ataagataaa tccataaatg tttaaaaaat
     1261 ttttaaatgc aggtgatcct aaaaatttat ctttagtttt tactgcgatt gtttctttat
     1321 tattaataat aaattttata ttatttatta gtattgattt acatgtattt atatggataa
     1381 ttattttatt aattgaaatt atttgtattt attttttatt taaaaaaata aatgtaaaat
     1441 ttatttatga taaaataaga ttgatttata aaaatatagc tactaagcga ttaggagacg
     1501 acaaacaacc aatacaagaa tgggtcaaag gtgatgtttt aaatcgtgtg cagcaagatg
     1561 tagaaaggtg gcttgaactt aaaaatttgg aaattgctac attaaaagaa ttagaaaatt
     1621 ttcgtaaaga ttttttagct aatgtttctc atgaacttaa aactccttta actacactgc
     1681 aaggatatgt agaaacactc atagatggtg ctatagcaga tccagaggtt aatctaaaat
     1741 atttgcaaag atcttctttc actatagaaa gaatgatttc tatggttgag gatttagaaa
     1801 ctatcactaa gctcgaacaa aactcagatc atttgaaaat tgaaaaattt gattttatct
     1861 ctttagtaaa agaagtttta gatatgatgg aactgaaggc aaatactaga aatattaaaa
     1921 tgattattca gaatcatacc actattcctg tatatgtttt agctgatgta gaaaaaatca
     1981 gacaagtttt agttaattta attgataatt ctataaaata tggtaaagaa tttggtaata
     2041 ccttcattaa aatttattat tttgagcaaa atttattgat tgaagtcgag gataatggaa
     2101 ttggtatacc agaagaacat ttacaaagaa tttttgaaag gttttatcga gtagataaaa
     2161 gtagaagtag agaagttggt ggcactggat tgggcttagc tatagtgaaa catattattg
     2221 aggctcatgg tcagcatatt aatctacgat caaaagtggg tataggtact acttttactt
     2281 ttactttgcc tttagcaagt actaagtagc cttatataaa aaaatttgtg agtattaaat
     2341 tataatttat tttaattttg aaaaaactat aaaaatgaat ttttctagaa tattaatttt
     2401 attatttata attttttcat ttcagagttg tgaatgtaat agaaaaaata aagataattc
     2461 atcaatagat aatatagatg ttagacagta tgaagagtct ttattagaag ctaataaact
     2521 atacctcgaa caagagcata gagatatttc tgcttatatt catcgaaaag ggtggcaaat
     2581 ggatacaacg ccacgaggtg tatattatat gatatattac agaggagatg gatatatacc
     2641 tgagcgtgga gacattgtta gatttgcttt caaaactatg cttatcaatg accaggtgtg
     2701 ttatgattct gaaatagatg gccccaaaga agtagagtta ggtaaagcag acatagaaag
     2761 tggtttagaa gaagttttat attatttacc cgaaggtacc aaagcttatg ttatcatccc
     2821 atctcattta gcatttggtt ggttgggaga tgtagaacgt atcccatcga gggctgttct
     2881 catttatgat ttgcatattt tgaaagtgat taaaatataa aaagattaaa actcttcgta
     2941 tgggactggc aaagggtgcc gagagttgta tcgtaaaaga aaattgtata taatcttctg
     3001 ctttttcttt gcatttttca gttcattata caaggcttca tccctttctt ttaaaaagtt
     3061 taaaaaatta ttataattaa aataaaacct caatccagtt tctaactcta caacatattc
     3121 tacatttact tttctgcgct ctgttactgg ataagaaacc caataattat aataaaaaaa
     3181 atcgtcataa gagtgataat atgatatctc aatgtcaaag aaatgtacta gtgcaccaat
     3241 aatagtagca cgataaaagc tgttttcgta agcttgctta atatatagag aattttcgaa
     3301 aacaaaagca aaaaaagtat ctatgctgca atatcttttt aagctgtcat ctttacaagg
     3361 cacttctatg tctaatatat caccatattt attttttaga atgtaaaaat ctgtataatc
     3421 aggtgtatta taatataatg attgtggttt gacatataca ccatcattaa acaaatattt
     3481 tctaattaca atatcatctt gagcatagta tacagtaggc aggattatta taaataaagt
     3541 gataaaaatt ttcaacttca tttattagaa ctttattgat ctaatttttg taaaaatgta
     3601 ggcttatgta tgatatagcg tttatatatt ccttctccta taagtccata agaatcgtaa
     3661 gcttttactt cgaaagttaa aacattacct tctattttga taagtttagc aatacttctt
     3721 acagtatcac ctatttttgt tgctttaatg tgtttaacat gaatttctat acctactgta
     3781 caataatcag ggtctaataa aggatctaca caatccatac aagttttttc cataaaagaa
     3841 actaaagcag gagtagaaaa tacatctact aaacctgagt catactgaat tgctgagtca
     3901 tcctgtccta ctaatttaaa tgcttcatgt gttaatccaa tttctaattc cataatatta
     3961 atttttttca gtgactccgg cgggactcaa acccacaacc ctctgatccg aagtcagatg
     4021 ctctattcac ttgagctacg gagccaatta ttacaaagtt aaatattttt tattaaatga
     4081 ataaattaat ttttaaaata aactttttga atatttaatt gtctaactta aaaataattg
     4141 acatgtatca attagtaata caaaaaagaa taacaattag agaaaatgaa gttttggctg
     4201 aatatctaga tgaagtttct caggagccat tactaactcc tgaggaagaa gtagagctag
     4261 ctaaaagaat aaaaatggga gataaagaag ctcgaaataa attaattaaa gccaatttaa
     4321 gatttgtcat atctgtagca aaacaatttt ctcagacagg attaccattg actgatttaa
     4381 ttaacgaagg taatataggt ttaataaaag cagcagaaaa atttgatgag acaaaaggtt
     4441 ttaaatttat ttcttacgcg gtatggtgga tacgacaagg tataatacaa gctttgtctg
     4501 agcatggtag aataattaga ttaccaggaa acaaattgac attaataaat aaagtaaaaa
     4561 atgcttataa tttattagag caaattaact cgagacctcc tacaatagat gaattatctg
     4621 atatgacaaa attatcacca gatcaagtaa aagaattatt aaattatgtg gataataatg
     4681 tagattcatt agacaatacc ataagcgaag aaagtgaaaa tactattata gattttatcc
     4741 ctaatcataa ttctccagct ccagatacaa atttagagca agaatcttta aaaatagaaa
     4801 tacaacgtat tttacaaata ttaccaatac gtgaacgaca aattatcaaa tactattatg
     4861 gacttgatgg tactcatgca ttaagtattg aagaaatagc taaacgaatg gatttatcta
     4921 ctgagcgtgt aagacaaata agagataaag cattaagaaa acttaaaaat aataaagaaa
     4981 ttaaattgct gaatcaatat attaattaaa ggaataaatt taagatattt tagttagttc
     5041 atttcttttt tataccaatg caaccaataa taattggcgg tcaaaattgc ggcataagcc
     5101 cacactgggt aagcaatttt atcagtatct aaaaaattat ttagtaaccc atgcaaaaaa
     5161 taggctaaaa gtgcaatagt tactgagagc actatcatgc ttctattagt atctaacttt
     5221 ttcaatttaa aaaacaatct aaaaccagta acaaaaagag aaattactat tgcaataacg
     5281 gtcagcatac caggaatacc agtttcggat aagggaccta gaaattcgct atgtgcattg
     5341 cctttagtgc cagtatttgt actaatatat gtttgatatt gaggtaattg atatggagca
     5401 tagataaatt gatatgttcc ggggccgcta cctactactg gatgatcttg agctattcga
     5461 atagctgata cccaacgatt tattctctca gtattgctca gatctgtact gatattagta
     5521 atagagcgaa cgtgggatgt tgcgtctttt cgagaaacat taggatttct ctccatttga
     5581 aacattattg attggtaatt tataaaaaat aaggtaaata ttatcactaa gcctaataga
     5641 acgtatctaa atttaatacc taataaaata ataaccccta ctactaatgc tattgcagta
     5701 ctaacccaag cagctctact agacgataat atcagcccta gagtcaatac taaaaaacaa
     5761 ataaaataaa gtatacgatt atttaaatga tttgttttat taaatgtttg cataaaagca
     5821 aaaggtatta ttagagataa agcagctccg tatatagtat gatccctata aaatggttga
     5881 gataccagat acgacatctt aaaatcaaaa ttatatcctg catgtttaat aactgctatt
     5941 atcactatta taactaatga gaataaatat aagtttaaaa atatattttg gtattttata
     6001 tctttgaaaa atactgatac acctaagaaa tatgcaggta tcattaacca cgcatgagct
     6061 aatacaaatt tccaagaaac taccggtatt tgactaaaaa aagcagaaaa tactagccaa
     6121 acaaaatata ttattatagt aatagtgata gaatgcttag aataaccaat aggtgaatta
     6181 ttagatataa atctatacag tgccaaaatc aacaatccta ataatatagg ttccgatggt
     6241 attgacaaag ctaaattaaa atctatattt tcaatagtaa tagaaaaagg aataataact
     6301 gctaaggcca aaaatatttt ttcgtaagaa aaaaaataat aataaagcaa taataataaa
     6361 aaaggtatta gggctacata atagaactca taacccatgg caatagtatt aacaacagct
     6421 aaaataatag ctgataaaaa aatccaaaat atagttttat taggtattgt ttttttagat
     6481 ttatctagca ctatatttta attttttctt atatttctca taattttcat aaaaaatcaa
     6541 acagaccagc gtgaggacaa aagcagctaa agcagaaact actactatca accaacgaac
     6601 aggatatgct ttgcggtcgg gtggatatgc atattctact acaaattttt gaggtatttt
     6661 actgaatgca tctattttag cctcatcata tttctgttga aattctatta atcttctatt
     6721 taaaagtgct aactgttcat ttatttgttt atataaagct ccatacttag tgatagtatc
     6781 taactgagct tgcaatatat tgacagcatg tgcgttgcct ctacctatct ctctaccaat
     6841 agcttcggtt aatctctcta cttgtaaatt ataatcgcct atgccttttt gatttaacca
     6901 ttttagagaa tcttcttttt gattgatttg ctgttgtatt tctagatatg tcccttctac
     6961 tattttgaaa ccttcaaggg ctcgttgacg ttgaattttt aatttcgtac tatcatataa
     7021 ctctgcaata gtattggcta tattagctgc taatattgga tcttcatctc ttacttttat
     7081 ctctacagac aaatattctg tcctgcgaaa ttttatatta ctctcatatt ctttatataa
     7141 agctgttcga gcatatttat atttgggatc tattttccaa tgattagcta agtcaaattt
     7201 tttaataatg gtatcgcgta tttcatttga tttcaatatc tgtatcattt gttgtgcttg
     7261 ctcttcttcg ccaaattgca atatatcttc tctgccacct tgatcggtta ttaatgcttt
     7321 ggatatagca ttactcatag tcgggaatag cgtaacctta gcttcatatt tttcctttat
     7381 taataatgat attatagctg ctattatagc tgctactaat gtggtaatta tcaatatttt
     7441 tctccattta atgaaaaata taaaaaaatt actactgtca aaatttactt gttcatcaat
     7501 agatggtttg aaatcttcca tatttaaatt tttattaaaa gcaaaattaa tagaaaatta
     7561 aaacatatta taaaatttac aataataaaa acccaaaaaa taaatttagt tattaactaa
     7621 atttattttt ttctaaccca gaaatcacaa ataatttgat tttaaaaaag gaatgtaaaa
     7681 gattgatgct gaaatagttg caatttttta taataatatt attcctcttc atcaaaaaat
     7741 tctttgaaat ctggataatc tggccatagg tcatctatac cttcaaattc tttttcttca
     7801 tcttctattt ctaaaagatt ttgtattaat tcaggaggag caccagttct atatgcatag
     7861 tctaataatt cttcttttgt tgctggccaa ggagcatctt ctaatttaga agctaattct
     7921 aatgtccaat acataaaatt tttcttttaa aaataatttg caaagatata tttttttttt
     7981 aaataaaaaa attaataaat tttcgtataa acagcattga tagtatcaat agtatttcca
     8041 tcatttacaa tataatcaaa attaatagta gtcttattat tttgcataag cccactacca
     8101 cgaacattta tagagttatt acatgtcaat tgtgaaggaa tatcaacagc gaggtcattg
     8161 acaattgctc taacaatttc attgttacca aattggtgga aattataaat atttatataa
     8221 ttcaatgtag tgctatctat ctttatcact acattataag caaaaccagt atttatttct
     8281 ttgcatttcc acgtgcctgc gaaatattct cttagatctt catcttcatt atcatttaca
     8341 caagctgtgg tgataaaaaa agtaaaaata agtaaaaata atgtatattt atttttaaac
     8401 atccgcatta acataattat taatttttac aaaactaatt aaaaaaaatt aaatattata
     8461 tattattctt ccaactttca attattattt atatattgat taaaatatta cttgtaattt
     8521 tgcaatatga ataaactaaa acgaaatttt tgtataatag ctcatataga tcatggtaaa
     8581 tcaactttgg cagatagatt gctggagcat acacatacta tagatcaaag aaagatgatg
     8641 gatcaagtat tagataatat ggacttagag agagagagag ggatcactat caaaagccat
     8701 gctattagaa tgcaatataa ttataaagga caaaattata gtttaaattt aatagataca
     8761 cctgggcatg tggatttttc ttatgaagtc tccagggcga tagcttcatg tgaaggagca
     8821 ttactagtaa tagacgcaac gcaaggtatt caggcgcaga ccattagtaa tctctaccaa
     8881 gccttagacc ataatttaga aatcatacca ataattaata aaattgatat gccaaatgca
     8941 gatcctgaag ttgtaaaaga tcaaataata gatttgatag gttgtagtag agatgatatt
     9001 atcgaagtta gtgctaaact gggtataggt ataaataaag ttttagaagc tatcatagag
     9061 aaaatacctc cacccactgg agactatgat ttaccattag aagctttaat atttgatagc
     9121 atattcaatc cttatagagg agtaatagca tattttagaa tttttaatgg tacactaaaa
     9181 actaacgata aaataatgtt ttactctaat aaaactattt atgaggcaga ggaaatcggt
     9241 tatttgggtt tttataatga accttgtaat gaaatgaaag ctggggatgt gggctatatt
     9301 attacaggta taaaatctac taaagatatt agagtaggtg atactattac tctagccgat
     9361 aatccttgta ataaagctat tgaaggattc aaacccgtta aacctatggt ttatgcagga
     9421 gtctatcctg tctcgccaga cgattatgat aatctcaggg aatctctaga aagattacaa
     9481 ctaaatgacc ctgcattaga atttacatta gaaacctcta acgctttggg atatggtttt
     9541 agatgtggtt ttttaggttt attgcacatg gaaattgtac aagaaagatt agaaagggaa
     9601 ttcaatattg atgtaataat tactgtacct aatgtctcat ataaaattta tactactaaa
     9661 ggagaaattg ttgagattca taatcccgtt ggattgcctg atccatctaa aatagatcgt
     9721 atcgaagagc catatataaa agcacaaatc attactaaaa gtgaatttat tggcaatata
     9781 attaagttat gtatagatag acgtggtgtt tttgtaggtc aggtatatct ctcttctgat
     9841 agggtagaat taacttttga gatgcctctt gtagagattt tatttgattt ttatgataag
     9901 ctaaaaagta tatcacgagg ttatgcttca tttgattatt tcgatatagg atatcgtgaa
     9961 actgatttag taaaattaga tattttatta aatggagaaa aagtagatgc tttatctata
    10021 ctgatacaca gaaacaatgc ttatagcttt ggcaggcgca tgtgcgaaaa gctaaaaaac
    10081 ttaataccac gccaccaatt tgatattgct atacaagctg ctataggtgg caaaattatt
    10141 gctcgtgaaa ctataaaagc ttatcgcaaa gatgtaacag ctaaatgcta tggtggcgat
    10201 atcacaagaa aaagaaaatt aatagaaaaa caaaaagaag gaaaaaaaag aatgagacaa
    10261 attggtaaaa ttgatgtccc tcaaagtgca tttttatcta tattcaaatt agattaatct
    10321 ttttacaagt tacactatgt ttttgaaata aattaaaatt tattctataa caattttttc
    10381 tatttaaaaa aaattacgaa atttgcactt taaaaaaaaa gtaatagata aaataaattt
    10441 atggcaacaa gaatcagatt acaacgaaga gggaaaaaac atcagcctta ttatacaata
    10501 gtagtggctg atagtagtgc accacgagat gggcggttta tcgaaaaact aggatattat
    10561 gatcctatga ctgatccagc tacaatagaa atagacattg atcgtgcatt atattggttg
    10621 caagtagggg ctaaacctag tgagacagca agatcacttt tgtctaacaa aggtgttatg
    10681 ctaaaatttc atttatggaa aggtatacaa aaaggtgcac tcactgagga agaagcagaa
    10741 caaaaatttc aacaatggct aaaagaaaaa gaagaaagag taaataaagc taaagaatct
    10801 aaaagacaaa aagaaaagga actatatcaa cgtaaacttg agcaagaaaa gcaagtgaga
    10861 ataaaaaaag aagaaatctt ggctgctaaa caagctgaaa aaactgaaaa agaagctcag
    10921 acagaagctc agacagaaaa tcatacagaa gaacccgcag aagtacctac tgaagaaaaa
    10981 actgatttag aagaagaaaa tactcaataa aaatttttta atctctatga tttttccaaa
    11041 taaaaattct cttatttcta ttggaaaaat agttaaatct tttggtatta atggccattt
    11101 attagtaaat tctgaatttt tagatgatta taattttaat aaaagaatag caatatttgt
    11161 ttattgggaa tattgcttcg taccactcat catagaggat ctagaaaata gaggtgataa
    11221 ttattatcta gtaaaattcg aattaataga taatgtagat gatgcatcaa aaataacggg
    11281 ttgcaatatt tatgccttta aagatgatat agaaatagaa aagatagatt atctgagctc
    11341 acccgagagg tttgtaggtt tcgatgtaat agatatctcc tatggattat taggtaaagt
    11401 aatcgggata ttgcgtggag catcaccaca attagaagtt tcgagtcatg aagattcatt
    11461 gttcatacaa ttgataccag aatatatgga tcatgtagat tgggataatg aaattatttt
    11521 tgtgaattta ccggagcaat tatttagttt ttatggcctc aagtaaccca ttctatttta
    11581 aaaaatttaa ggtttatgat gataaatcaa cgatgagagt aaatacagat ggtgtactac
    11641 tcggagcttt ggttacccca tcaaatgata aaaatatact agaaattggt tgtggagcag
    11701 gatatatatc tatgatgtta gcacaaaaaa atccttattc gctaatacat gcaatagata
    11761 tcgatagtga tacagtagct caagcaaatt acaattttag attatgtaaa tgggctaata
    11821 gacttgcagc atataatatc gcattagaag actattctaa tatctctcat caaaaatata
    11881 atcttatagt ctccaatcca ccctttcatt tagaaggtat gaagtctata aatactaaaa
    11941 aatccttagc caagcatact aatattgagg gtattaataa tttttttgct gctatttcta
    12001 atttattagt aaagagtgga gaattatgga ttataatttc tgcaaagtcc tataaaacta
    12061 taatatctat cgctagagaa cattatttat ttcctaataa gcaaataaat attaaatcaa
    12121 taaaaactaa gagtaactca aaagtagttg taagttttcg taaacaaatt acctacccat
    12181 ttgacaattt agaattagta ataacaaatg aaaatcaaat ttatacagat gaatttatag
    12241 aattgatgaa agattactta ttattaaaaa aataaaaatt ttaaattttt agcaataata
    12301 gaggttattc aatatttcta ttatctttaa ttaaaataga tttagactct tgagtttgtt
    12361 taatatttat atttttagag tttatatgaa taaatgaaaa catcttgatt attccttaat
    12421 ttatttacat tgagcaaaag tgatattgct gatataaagt ccattaattt attttcaatt
    12481 aattttgtag taattagtaa taaaaaagct atgaaaataa tttttagatt tttttttgtc
    12541 ttattaattt ttattaatgg attaaatgct caaaatatta attataattg ctttactatc
    12601 ggtgataatg gcaatgatga agctaaaact gctataatta ccagtgatgg aggttatcta
    12661 atcggaggtt ggacaaattc atataatact gatgactacg atatttattt gataaaatta
    12721 agtggaaaaa ataatataga atggtctatt acaataggtg gtactgagca cgaagctgcc
    12781 aatagtatca agcaaactaa tgatggaggt tatgtcattg ctggttatac taattcttat
    12841 ggttttggga gcttcgatgt atatattgtg aaaatagata aaaatggaaa tatagaatgg
    12901 acaagagtaa taggtggtga taaagatgat agggctaatg atatagctca gactgatgat
    12961 ggagggtatc taatcgcggg taccacttat tcatatggcg gggggtatag cgatatttat
    13021 ataataaagt taaataattt aggcgagata atgtgggaaa aaacaatagg cggtaaagga
    13081 tatgatggtg ccaatattat aattaacagc aatgagggcg gttatttagt gggtggttat
    13141 actagctcct atggtagtgg tggcgaggat atatatttga taaagataag taatttaggc
    13201 gatttagaat ggactcgaac aataggtggt aatggtgatg agaatgctta tgatatcata
    13261 caaaatgatg ataatgacta tatattggtc gggcatacca cttcgtatat agaatatgaa
    13321 gatgattttt atatagtaaa aatggatgac aatggcaatg taaagtggac taaatctatt
    13381 ggtggtatag agcatgaaaa tgcaatttcc attttagaag ttaatgatgg tggtttcata
    13441 atagttggga gtacttttac ttatggtgct gggcttcacg atttttatgt aacaaaaatt
    13501 aatagctacg ctgaggtgga atggataaaa tgtttcggga cagataaaaa tgataaagca
    13561 gctactatta taaaaacaaa ctatgataat tttttgataa ttggtaatac agaaaccaaa
    13621 aattatgata aagacatact gataatacag atagataaaa atggtaatgg ctgttgtgat
    13681 tctgaatcag gtggtgagat aaaagttggc ggtgtaggga gtggtggtac aataaaagat
    13741 gtaattagcc aaactactac tggcggaaat atttcaaatg ttaatagttc aaaaaaagaa
    13801 atatgccagt agtaatttat attaaataaa aagtatttat atagctaaaa tatacatcat
    13861 ttctattaaa tctttatcaa ccgctttaaa atttttcaca gctctttcta atggaatttt
    13921 cactatttga tcattcatta tcccaatcat tattttatct tcacctatta ttagagattt
    13981 gacggcatgg tatcccattc tggaggcatt taatctatct cgcattgtgg ggttaccgcc
    14041 tctctgtata tgtcctagaa tagtatatct gatttcataa tctggaagtt tttctttgac
    14101 aatttgagac acctttacag cacctccagt ttcttcacct tcggctacga tgataataga
    14161 acttttttta tttattctcc atctattttt aatattatct accaaatcat ctatattagt
    14221 tttagtctca ggtattaaaa tctcttcagc tccattagca attccagtat ataaagctat
    14281 ataccctgca tctctaccca ttacttctac aaaaaatatt ctactatgag aactagctgt
    14341 atctcttatt ttatccactg cttctacaac ggtattgcaa gcagtatcaa aaccaatagt
    14401 ataatctgta ccatataaat cattatcgat agtactaggg attcctataa tatttatatc
    14461 aggatattca tttttaaatt cacaagctcc tttaaatgta ccatcccctc cgattactat
    14521 taacgaaccg atatctttgc tacataaatt tttgtaagct ttttgtctat gttttttttc
    14581 taaaaatttt tgactacgtg cagagcgaag tatagtacct cccctttgta ttatattgct
    14641 cacatcacta gcttgcattt taaatatatc accttctatc aatccatgat aaccctcata
    14701 tataccatat atatctctgc cattatagat agatgtgcgt actatggctc taatagcagc
    14761 attcatgcct ggtgcatctc cgccagatgt caatactcca atatttttca tttttttaca
    14821 aatttaatta taaaaatagt taatatctta aagatttaca aatataattc ttttgacata
    14881 acaattaaat ttttcattac aaatgagatg attaattaaa taaaatatat tttaattatt
    14941 tacattataa aatatttcct gtaattttgc aaatattaat aataaataga tcatgaaaga
    15001 ttcaagagat ttaccacgca ttactacata cgtgttaaag gaaatgaaaa tgcgaggtga
    15061 aaagataagt atgctaacag cttatgatta tagttttgct aaaattttgg ataaagctgg
    15121 aatagatgtc atcttagtag gagattcagc agcaaatgtt atggctggct attctactac
    15181 attgcctatc actttagagc aaatgattta tcatggtgca tcagtagtca aaggagttaa
    15241 gcgttcttta gtggttgtag atatgccttt tggaagctat cagggaaatt caactttagc
    15301 tctagagtca gctattaaga taatgaaaga gacaggagca caggctgtta aattagaagg
    15361 gggtagagaa atattagagg caattgatcg tattttgtca gctggtatac ctgtgatggg
    15421 acatttgggt ttaacacctc aaagtatcaa taaatttggt acatatactg tgagagctac
    15481 tgaaaaagaa gaagctgaaa aactcagaga agatgctatg ctactcgaaa gagctggttg
    15541 cttcggtata gtattagaaa aaattcctgc tttattagca aaggaagttg ctgagtctag
    15601 cagtgttccc ataattggta ttggagcagg acgctgggta gatggtcaag tactagtttt
    15661 gcacgatatg ttaggaataa atcaagattt ttcacctcgc tttttacgaa gatatcataa
    15721 tttagaagct gaaattttaa aagcagtaaa tcaatatatt gatgatgtaa aaaatcttga
    15781 ttttccaaat gaatatgaac aatattaatg attggaaagt tccaatacca attttatatg
    15841 aagataatca cctaatagct gtacataaac ctcatggact tctaatccaa ggagataaat
    15901 caggagatat cacattaata gatcttgtaa aaacatatct caaagaaaaa tatcaaaaac
    15961 caggtgaggc ttttctaggt ctaatacata gaattgatag gcctacagga ggtattgtta
    16021 tttttgctaa aacatctaaa gcccttacaa aaatgaataa aatttttcat gataggcaag
    16081 ttaagaaaat atatttggct atagtagaaa ataaaccaac aatagataat gggttgctag
    16141 tacattattt aagaaaaaat gaaaagaata ataaaagtta tgtttctgat atacacaaaa
    16201 ataattataa aaaagcagaa ttatatttaa attatataac atcatctagt aaataccatc
    16261 ttttaggcat agaattgttg acaggaagac atcatcagat acgtgctcaa ttatctcata
    16321 ttgcatgtcc tattagagga gatctgaaat atggcgctcg tagatcactt ccaaatggca
    16381 atatagatct cttatcatac tatttggagt ttatacatcc aataacaggt aataaaattt
    16441 ctattacaac aaaaatacct gaatataatc catggcaatt ttttaaaata ccggcaaaaa
    16501 aatggttaga taaagtttat ttttagtaag ggtgaagtgg tgagatgtgt atgtataaag
    16561 tgataatatt cttaggtgtt taaatataca caggacttgc tctcaagtcc ctatatacat
    16621 accacgctcg cctgtctgat ga
//
LOCUS       DAOPRN010000041        16257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_3017_length_16257_cov_99.578015, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000041 DAOPRN010000000
VERSION     DAOPRN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16257
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3017_length_16257_cov_99.578015"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1139
                     /locus_tag="PKI65_05720"
     CDS             279..1139
                     /locus_tag="PKI65_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOB77973.1"
                     /translation="MQINIVIKKVVIVFAISIFSISVYSQFYVNVNGGYSLGVANQTM
                     IPNYEALYDEDIEKFESVYGSLGKGANFGLNLGYMFNNNIGIDIGFSFLMSDQIKSEY
                     KEKYTNWQNNDIFCSEKISLRNQMIRINPSLIIAAGFEKVEPYAKFGVIIGLGHINLK
                     YTEEYFINSSLDDQMIEELKLDGGIAFGMSSAAGLMFHLSDIITIFSEFNLVSMSYAP
                     EKGKITKYSKNGIDLLPDLNVEDKEIDFVDSYTYDYDNPPSSNEPRKMFKIKFPYSGI
                     GLNVGIRFSF"
     gene            1203..1853
                     /locus_tag="PKI65_05725"
     CDS             1203..1853
                     /locus_tag="PKI65_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOB77974.1"
                     /translation="MKNNKLFVITILFCIVCNVNYSQNFNILIKGGLNLSNMIVKDDD
                     KTLSDDYKMLPGFHIHPAIEYSFSDLFSLEGGIAFQSRGYKVKGDNHKFRLTSIYLDI
                     PINAKFNFNLGNVNLFCSLGPYVAFGIGGKIYKEGTVHNITKITKRPIKWGSDWDDDL
                     KTVDYGLNFGVGLDINNFMVGMSYGWGLANLCTIDDLDYKIKNYVLQISVGYKFNL"
     gene            1911..2543
                     /locus_tag="PKI65_05730"
     CDS             1911..2543
                     /locus_tag="PKI65_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOB77975.1"
                     /translation="MKKIYSLIFVTIIVFSTTMFAQNLSLGIKAGLNLSNVISKDDDI
                     TYSEDLNMLIGFHANPFIEYSFSNLFSLEGGISLQSKGYSDKEDIYKTRMFLLYLDIP
                     INAKFSFDLGKADIYCYAGPYIGFGLSGSEKVLVDSEVRYDYTIDWGSDTKDDLKRVD
                     YGLNIGVGLDISNFLIGVNYSWGLADISNFTGIKIKNYIIGISIGYKFGL"
     gene            2696..3331
                     /locus_tag="PKI65_05735"
     CDS             2696..3331
                     /locus_tag="PKI65_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOB77976.1"
                     /translation="MNQNKIIYFLIVLFLIIGCKKVEKVYYPDGSLAAEYSIKNNKKE
                     GIAYQYDKMGNKIAEMNYKNDFLDGFYYEYYPNGTIKSKIEYKENKKNGKAFYYDLKG
                     NIVEELTYKNDTLNGIYKEYYSNTLIKKEGNYKNGKYDGAWYYYNEKGYLTGEGHFDN
                     GNGELVSYYPYSDIIHSVTPFINNQKNGWEVVLSPNGDTLLKNYYENDIKI"
     gene            complement(3457..6060)
                     /locus_tag="PKI65_05740"
     CDS             complement(3457..6060)
                     /locus_tag="PKI65_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB77977.1"
                     /translation="MKRINLILLILFSIITKGLGQNPTINLDASTHNTQKAVCGYWFY
                     DDGGPGGNYSNNQDRWITFYSTDPVYTHIKIDFVYVTLAAGDTLIIYDGPDINSPVLI
                     RYSQANPLTPANSSVQATVNNPSGKLTVRFKADATGNAAGWDAAIGCIKMCQRIIPVL
                     DSALTSPLPLPDYYINLCASDTLTLAVKTDSTAFPDNHIIYDQTLSNTTFAWNMGDGN
                     TLYGPVVSHHYDIPSGYNVSLLITDTMGCINTLSLSFRVRISDNPIASIDDAVPDICV
                     GDSIILTGGIGSGNTVVVNPFSNVITAQGVYDSVTFIPDGPNCPTLCYGTPVNFNQFP
                     PGATVQSASDIESICIFMEHSYAGDLSFRIICPSGQSVVLDSYDNSGSNYLGEAYDDY
                     GGSCDPAQNPQGIPWLYCWSEISTYQYQGLLNVVDGSGPSPIPATDTINHTNYLIPEN
                     PLSGLVGCPLNGIWSIEICDHWGIDNGWVFEWMLTLSSNSGAAGWEYTVNIDSIVWNG
                     PYINIINDSTAIFKPDSSGTFNFYITVVDEYGCSYTSFTPLEINTVEVPDIELGADTM
                     LCEGMPLILNAGGGYDEYIWSNNTRDSVLFVEMSGIYSVTATNFNEAQTLGCFGSDTI
                     KVTFIPNAYVDLGPDLCLLEGPVTLDATYAPGFSYLWSTGANTPIIHLNEPGIYDISV
                     VVFYEDPSMCAGHDTVKIKLIPTPIIELPDTMFMCAHQARIISVEQMYDYLYNFEWST
                     GNTTPTQIFQNMLPGIYDIQVKVIGCDTTIHETRLTVDDCAIMIPNVITPNGDGINDY
                     FQITNIDYYPNSEIKIYNRWGKRVYESQNYQGDWDGEGLDEGTYYYIFRLNYGNGQIS
                     EYHGTITILRN"
     gene            complement(6078..6488)
                     /locus_tag="PKI65_05745"
     CDS             complement(6078..6488)
                     /locus_tag="PKI65_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77978.1"
                     /translation="MKKVFVAILISLFTLVSYSQEFDPRLEKHFGKDKLLYWQKNYPD
                     SLGYYEFVIDNAFLIMDEKIVSARLGDEKVKEIEIPNFEDILCNHQLMDIFSLGIQID
                     PNRYQYIKIKDEPYYLYIKPGDYLWNKYQAGKNN"
     gene            6810..7463
                     /locus_tag="PKI65_05750"
     CDS             6810..7463
                     /locus_tag="PKI65_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOB77979.1"
                     /translation="MAIPAKYIHKIAQMFTRYGIRNVTMDHIAREIGVSKKTLYTWTD
                     SKENVLSEIIDYILTIATNNECENSFKKTPKDFPTSIDAILYVMNNLAHITQNITPIF
                     IMELNKYYPQLAIKLDNFRYRHIREKIMQNIVRGKKEGLYRSDINEEIISYFYLGWVS
                     QFSNENMQTVFENKYSNQEILHELYLFHLNALLSEKGRKYLEQKLENYNKDENANNN"
     gene            7441..8787
                     /locus_tag="PKI65_05755"
     CDS             7441..8787
                     /locus_tag="PKI65_05755"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOB77980.1"
                     /translation="MKMLITTKKFFFSIILLSSFILVNGQNNNVQLTLQQAHDLALQQ
                     NKSIQAASMDKIIAQKTKWETITNYFPKASASAKLLDNLKLQTVLLPAVMIGGAPGEY
                     IPVQFGMEFQTSWSIEVQQLIFSLPLIIGIQLSNLGAEMAELGYLNTEREVVSSVNSV
                     YTSALVLKHNLNILDSNIVNLQNVRQQTQAMYEVGVLQSTDVDQIDLTIMNIENTKKA
                     LERSLELSYNVLRFQLGLDTNVVFELTTDINDILNEGEINNLILTPVDIYKNIDYQFV
                     EKQRDMALLSLRKEKSDFAPTIAGFYTYSEAGQGNKLNDLRWFPASIAGLTISLPLIN
                     GGQNGVQVSKAKVELQKSNLTLDMVNEQLKIQEKQLKYNLSNAYENYLTQKKNIEVSN
                     RVYKNIEMRYLQGVASSLELTQANTNYLTAQSNYLSSVMQLLNAKYELEKLLYNQK"
     gene            8794..9846
                     /locus_tag="PKI65_05760"
     CDS             8794..9846
                     /locus_tag="PKI65_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOB77981.1"
                     /translation="MKIKNLILVIVLGTGIFLNVSCASKSSQKESDSDDIYNVQVAKL
                     EKQILDNTLEFTANIEAWEKVNLAPASPGRIIKIYAEVGNHIKKGQLLVNMDPTQYNQ
                     ARLQLTQLQIDYNRMDSLVKVGAISRQQYDQIKTQYDIARNNTEFLEQNVNLKAPFSG
                     VITGKYFENGELFSGAPNTKEGKAAILTLEKMNVLKAYVDIPESYYNNIKLNLPVEIK
                     VDALGDQKFNGKVSQIYPTVDPSSRTFRVEIKIDNPREIIKPGMFARLSINMGRIEAL
                     AAPSIAVLKLQGTNKYYIYKVENNIAKQVFIERGRILENYTEIANGNIKEGDYIVVVG
                     QDKLFDGAKLNIINNK"
     gene            9879..13049
                     /locus_tag="PKI65_05765"
     CDS             9879..13049
                     /locus_tag="PKI65_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOB77982.1"
                     /translation="MKIYETSVKRPITTLMIFLAIIVFGVYSFSRLAIDFFPEIELPT
                     ISIITPYIGADAASIEKNITKPLEDRLSGVNDLDEIYSTSRDNLSIITLKFIYGTDLN
                     EAANDARNIIDMTMRFLPEDVDRPQILKFSTSQMPVIVYGVQADQNYEGLDKIIENNI
                     TNNLNRINGVASVLVRGAPERVVYVEFDPKKLEAYNITLDQVAAVVSRENIDVPVGDI
                     ETKDMEYNVTTAGEFISSDEIKNLIVGFQNGKFVKISDIAVVRDSLRDQKTIDRVNES
                     RGVNISIMKQSDANTVQVSRKVKNELEKITKTLPADVKIFPIYDSSENIQNSINNLSE
                     TLLFAFIFVSLVIFLFLGKWSSSLIVLVTIPASLISGFIYLFLAKDTLNIISLSAITI
                     AIGMVVDDAIVVLENITKHIERGSTPYEASIYGTNEVWTAVVASTLVILVVFMPLTLL
                     TGITGTIFRSLGWIVSITIAMSIICAITLTPMLSSRLLKSSSLQYQDNNGKKKKKNLW
                     QSTMGVVLDKLDNAYHKSLTYVLKHKTATLLIALAIFVGTMFLVPMLGFEFLPEADQD
                     ILRGTIYLQTGGNIDKTAEFVSKLEKTVNERYPEKYILSISAGVPSDQTASLTGTKAS
                     NEIQVTLRLKKREERERTVFEIAEDFRQFLDASPEVIKYDISAGGMGGMTNTVDVKIF
                     GYNIDSSLKVASELQYQISKIPGARDVIIDRANDRPTFNIYVDREKAAKLGLNTAMIG
                     SVVRNYITGYTASKYKEEGYEYDVIFRLSDVARKDVEKFKNLMITTPLGTKVCLSEVA
                     NIVEETTPLEISHENKQRVITLNAKPEGISLGELGSSINKIIDNASFPEGITAEVGGS
                     YENLTDSFRDLITLLFVAILLVYIVLAAQFESTSMPLIILFALPFAFSGVVLALLITG
                     NTLSVNAMLGAIMLVGIAVKNSIILVDYTNLLRDRGMRLNDAVVLGGRSRLRPILMTA
                     TTTFLGMLPMALSTGEGSELWAPIGIAVIGGLVFSTILSLFVVPVAYHTFIRSGSRRK
                     EIMRARRQFKFIDEIVINNKDDNNNK"
     gene            13111..13359
                     /locus_tag="PKI65_05770"
     CDS             13111..13359
                     /locus_tag="PKI65_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77983.1"
                     /translation="MLERLDIKAYTKWETVIGKGTVGEPRFGTHTWPELNSALISVVD
                     DEKVQPFLSAIHKLDKINEGAGIKAFVWDVLMSSDDVE"
     gene            complement(13580..15961)
                     /locus_tag="PKI65_05775"
     CDS             complement(13580..15961)
                     /locus_tag="PKI65_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOB77984.1"
                     /translation="MIFYPEDLPQKIYFDFIKSQLSMYCETLYGRDFIDLLSPTNDHK
                     KIINEYEALKSLKKYFQQNNIFSTKINGDIRSELEKLATEGFVISQEMLNSLLQLLTI
                     YINLNKIINDKKLSILRSQHVKFNEADQILEQLKHIWSYEGYIRDDASEELASIRRRI
                     DSMQRNIDKQIKEIWKQWQKKGFVADDSNVTLRDQRLVIPINPEAKDSVKGVIYDESA
                     TGQTIYIEPQEIFELNTSLQHLYFDEKKEIYHILRQFTDQLMPYIKTLLLIIDEITYL
                     DVLKAKYLWSTDNNAIIPEITDEPEINLINAYHPYLNHVLTTHNKKIVPLTINLTQNN
                     RFLIISGPNAGGKSVVLLTVATMQWMVQCAIPITAHEGSRLYIFSHIFMDMGDNQSLE
                     NEISTYASHLLNMKYILENSNDKSLILMDEMGTGTEPIMGGTIAELVLERLYQNNCFA
                     IVTTHFHNLKLLPMRYPAMQNAALLFDTDTMSPTYILSQGIPGNSFTLEIAQKVGFPQ
                     DIINKAQNQLGDNRIETEKLLVFLENERKKLEEQKKQLELAENFVAELIKKYQDNLEF
                     QKKKQNYFIKKTKGEIDEIFSKSNKLIENTIREIKESNAEKNKVKDIRKKLEKEKNTI
                     DKKLEKMLVADTIKEEEKIEFEVGHLVKIKLTGEVGEIIRISENQLNVVVNNKIIQLP
                     ISAVDYIDKNEQYINKKIKVNLVSRTESFNTVLDIRGCRADEAINKLDKYIDQALLIG
                     VPRVYIIHGKGYGILRKAVRDYLKDLTHLLNFENEVEERGGDGITVVNFTNQK"
BASE COUNT         5738 a         2152 c         2554 g         5813 t
ORIGIN      
        1 atccataatt atttttttgt aaaattaaat aaaaattatt tgatggtagt ataaactacg
       61 taaagtaaaa caaagatgaa tttcaaaaaa cctcgcttaa caaatacctt ttatgaagcc
      121 taattatttt attatacttt tttttaaaca tattttatct tgaatagatc taaaaatata
      181 ttaaacatat gataccttgt atattattta tatttttttg tttattaaaa tgcattttta
      241 tatttttgca aaagtaaatc atttaaaatc tgaaaaacat gcaaataaat attgtaatta
      301 aaaaagtagt aatcgtgttt gctattagta tattcagtat atctgtttat tcacaatttt
      361 atgtcaatgt aaatggagga tattcattgg gcgtagctaa tcagacaatg ataccaaatt
      421 atgaagcttt atacgatgag gatattgaaa aatttgaatc agtatatggt tcattgggta
      481 agggcgctaa ttttgggcta aaccttggct atatgtttaa taataatata ggtatagata
      541 taggcttttc atttttgatg agtgatcaga taaaatctga atataaagaa aaatatacaa
      601 attggcagaa taatgatatt ttttgcagcg aaaaaatatc acttagaaat caaatgatta
      661 gaattaatcc ctctctaata atagctgctg gattcgaaaa agtggaacct tatgctaaat
      721 ttggtgtaat aattggttta ggacatatta atttaaaata tactgaggaa tattttatta
      781 attcttcttt agatgatcaa atgattgaag aattaaaact cgatggtggt atagcttttg
      841 gtatgtcatc agcagcaggt cttatgtttc atttatcaga tataattaca atttttagtg
      901 aattcaactt agtgagtatg agttatgctc ctgaaaaggg taaaataact aagtacagta
      961 aaaacggcat tgatttatta ccagatttga atgtagagga taaagaaata gactttgtag
     1021 atagttatac ttacgattat gataatcctc catcatcaaa tgaaccaagg aaaatgttta
     1081 aaattaaatt cccatatagt ggtataggat taaatgtagg aattagattt tctttttaaa
     1141 tttttaggat agatgcatta gttttttttt taaaaaaata ttgtttaatt ttgaaacaaa
     1201 aaatgaaaaa taataaatta tttgtaatta caattttatt ttgcattgtt tgtaatgtta
     1261 attattcgca aaatttcaac attctcatta aaggaggatt aaatctatct aatatgatag
     1321 taaaagatga tgataagacc ttgagtgatg attacaaaat gcttcctggc tttcatattc
     1381 atcccgccat agagtattct ttttctgatc ttttttcttt agaaggtggc atcgcttttc
     1441 aatcaagagg ttacaaagta aagggggata atcataagtt tagactaact tcaatatatt
     1501 tagatatccc aatcaatgct aagtttaatt ttaatttagg aaatgtaaat cttttctgta
     1561 gcttgggtcc gtatgtagct ttcggaattg gtggaaaaat ttataaagaa ggcactgttc
     1621 ataatataac aaaaatcact aaaagaccta taaaatgggg atcagattgg gatgatgatc
     1681 ttaaaacagt tgattatgga ttaaattttg gggtggggct cgatattaat aattttatgg
     1741 tagggatgtc ttatgggtgg ggacttgcta atctatgtac cattgatgat ttagattata
     1801 aaattaaaaa ttatgtttta cagatttctg ttggatataa atttaattta tgaattttat
     1861 ttgtataaat taaaattatt attatttttg tagaaaataa taataaagat atgaaaaaaa
     1921 tttatagttt aatttttgta acaatcattg ttttttctac tacaatgttt gcacagaatc
     1981 ttagtcttgg tataaaggct ggcttaaacc tttcaaacgt aatatcaaaa gatgatgata
     2041 ttacttatag tgaagatctc aatatgctca ttggttttca cgctaatcct tttatagagt
     2101 attctttttc taatcttttt tcattagaag gtggtatctc attgcaatct aaaggatata
     2161 gtgataaaga ggatatttat aaaactagaa tgtttttatt gtatttagat atacctatta
     2221 atgcaaaatt ttcttttgat ttaggaaaag cagatattta ttgttatgca ggtccttata
     2281 ttggttttgg tttatctggt agcgaaaaag tgcttgtaga ttcagaagta cgttatgact
     2341 atactatcga ttggggatca gatactaaag atgatttaaa aagagtcgat tatggtctaa
     2401 atataggcgt aggtttagat attagtaatt ttctaattgg tgtaaattat agttggggcc
     2461 tagctgatat ttctaatttt actggaataa aaattaaaaa ttatataata ggtatttcta
     2521 ttggttataa atttggatta taatatttaa atatcgttat atatcacttt tagtcaattt
     2581 ttttttggca ataaatgtaa tgttatattt ttactatata taaattttag aattatatat
     2641 ctaattactt tatcagattt ataattaatt tataaatttg ttattaaaaa attttatgaa
     2701 tcaaaataaa ataatttatt ttttaatagt cttatttcta ataataggtt gtaaaaaagt
     2761 ggaaaaggtt tattatcctg atggctcatt agctgctgaa tattctatta aaaacaataa
     2821 aaaagaggga atcgcttatc aatacgataa aatgggaaat aaaattgccg aaatgaatta
     2881 taaaaatgat tttttagatg gattttatta tgaatattat ccaaatggga ctataaaatc
     2941 taaaatagaa tataaagaaa ataaaaaaaa tggtaaagca ttttattatg atttaaaagg
     3001 taatatagta gaagaattaa cttataaaaa tgatactcta aatgggatat acaaagaata
     3061 ttattctaat actttaataa aaaaagaagg taattataaa aatggcaaat atgacggggc
     3121 ctggtattat tataatgaaa aaggatattt aactggtgaa ggacattttg ataatggtaa
     3181 tggtgaatta gtttcttatt acccttattc agatataata cattctgtta ctccttttat
     3241 taataatcaa aaaaatggat gggaggtcgt attatctcct aatggggata ctttattaaa
     3301 aaattactat gaaaatgata ttaaaattta aaacgtaata atttcattaa ttaaacaaca
     3361 attaagagta ggaaagctgt agacagctaa agtaaaagaa agatgtgagt ttggtttaga
     3421 ttatttatga cattgatata gtgtagctct atatatttaa tttctcaaga tagtaatagt
     3481 gccatgatat tcgcttatct gaccatttcc ataattaagt ctgaaaatat aataataggt
     3541 gccctcatct aatccttcgc catcccaatc gccttgataa ttttgacttt cataaactct
     3601 tttaccccag cgattataaa ttttaatttc actattagga taataatcta tattagttat
     3661 ctgaaaataa tcatttatac catcaccatt aggagttatc acatttggta tcattattgc
     3721 acaatcatcc acagtcaatc ttgtttcatg gatagtagta tcacaaccta ttacctttac
     3781 ttgtatatca tatatacctg gtagcatatt ttgaaatatt tgagttggag tagtattgcc
     3841 agtagaccat tcaaaattat aaagataatc atacatttgc tctactgata tgattctagc
     3901 ttgatgtgca cacataaaca tagtatctgg aagttcaata attggtgtgg ggattaattt
     3961 aatttttaca gtatcatgtc cggcgcacat agaaggatct tcataaaaaa ctacaactga
     4021 aatatcataa ataccaggtt cattcagatg tataattggt gtgtttgctc ctgtagacca
     4081 tagatatgaa aatcctggtg cataagtagc atctaatgta acaggtccct ctaataaaca
     4141 tagatctgga cctaaatcta cataagcatt tggtatgaaa gttactttta tagtatcact
     4201 gccaaagcaa cctaatgttt gagcttcatt aaaattagtt gctgttacag aataaatacc
     4261 tgacatctct acaaataaga cagaatccct agtattatta ctccaaatat attcatcata
     4321 accaccgcca gcatttaaaa ttaatggcat accctcgcat agcattgtat cagcacctag
     4381 ttctatatct ggaacttcta cagtgttgat ttctaaaggt gtaaatgatg tgtatgagca
     4441 gccatattca tctactactg ttatataaaa attaaaagta ccactactat caggtttaaa
     4501 aattgctgta gagtcattga taatattaat ataaggtcca ttccaaacaa tggaatctat
     4561 attcactgta tattcccagc cagcagcccc agaattgcta gatagtgtta gcatccattc
     4621 gaatacccag ccattatcta ttccccagtg gtcacaaatc tctatgctcc aaataccatt
     4681 taacggacac cctaccaaac cacttagagg attttctggg ataagataat tagtatggtt
     4741 tatagtatca gtggcaggta taggactagg accagagcca tctactacat ttaataaacc
     4801 ttgatattgg taagtggata tttctgacca acaatataac cacggtatgc cttggggatt
     4861 ttgagctgga tcacaactgc caccataatc atcataggct tcgcctaaat aattactacc
     4921 actattatca tatgaatcta gtaccacact ctgaccactt ggacatatga ttctaaaaga
     4981 aagatcacct gcgtaagaat gttccataaa gatacaaata gactctatat cactagcaga
     5041 ttgcacagtg gcaccgggag gaaactgatt aaagttaaca ggagtaccat aacatagtgt
     5101 tgggcaatta ggaccatcag gtatgaaagt tacagaatca tagacacctt gtgcagttat
     5161 aacattacta aatggattta ccacaacagt attgccacta ccaataccac cagttaatat
     5221 gatagagtct cctacgcata tatctggtac tgcatcatct atactggcaa taggattatc
     5281 actaatacgt actctgaaac ttaatgataa tgtatttata caacccattg tgtcagttat
     5341 caagagagat acattataac cagaaggtat atcatagtga tggctcacca caggcccata
     5401 taaagtatta ccatcaccca tgttccatgc aaaggtagta tttgacaaag tctgatcata
     5461 aattatatga ttatctggaa aggctgttga atcagtctta acagctaaag taagagtatc
     5521 agaagcacac agattaatat aataatcagg tagaggcaat ggtgatgtta atgcactatc
     5581 taatactggt attatccttt ggcacatctt gatgcatcca atagctgcat cccatccagc
     5641 agcattgcct gtggcatcag ctttgaaacg tactgttaat ttgccacttg gattatttac
     5701 agttgcttgt acagacgagt tagctggagt taatggattg gcttgagaat atctaattaa
     5761 aactggtgaa ttgatatctg gaccatcata aatgatcaag gtatcgcctg ctgctaaagt
     5821 tacatataca aagtctattt ttatatgtgt gtatacaggg tcagtggaat aaaaagtaat
     5881 ccatctatct tgattattag agtaattacc accaggccca ccatcatcat aaaaccaata
     5941 accgcagacc gctttttgtg tattatgtgt ggaagcatct agattgattg ttggattttg
     6001 tcctaaaccc ttagtaatta ttgaaaacaa aattaataaa ataagattta tacgtttcat
     6061 aattttccct ctttttttta attattttta ccagcttgat atttattcca taaataatct
     6121 ccaggtttaa tatacaaata gtatggctca tcttttattt ttatgtattg gtatctattg
     6181 ggatcaattt gtataccaag tgaaaaaata tccatcagtt ggtgattaca aagtatgtct
     6241 tcgaagttag gtatttctat ttcttttact ttttcatctc ccagcctagc acttactatt
     6301 ttttcatcca ttattagaaa agcattatct attacaaact cgtagtaacc taaactatca
     6361 gggtaatttt tttgccaata caataattta tctttgccaa agtgcttttc taatctagga
     6421 tcaaattctt gagaatatga tacaagagta aataagctta ttaaaattgc aacaaatact
     6481 tttttcataa taatttaata tttttatata ttagacaatg caaatttaca aaagtttatt
     6541 gattaaatta taattttact gtctacatta aaaaaatcga catattttag ttttgcaaaa
     6601 tatgtttaaa aaatatagta atcaagaata aaagtgtagg atagaattaa attagtttta
     6661 taaattttta tctaaaaatt tataatttaa agctaatatt ctctattttt cataataaat
     6721 ttttttgatt taaggatact ttggaaactt ttttacaaaa aaagtttctt atgtgaaatt
     6781 attattatat ctttgcaaaa atttattaga tggctatacc tgctaaatat attcataaaa
     6841 tagctcagat gtttactaga tatggaatcc gtaatgttac tatggatcat atagcacgag
     6901 agataggagt atctaaaaaa acattatata cctggacaga tagtaaagaa aatgttttat
     6961 ctgagatcat tgattatatt ttgacaatag caactaataa tgaatgtgaa aatagtttta
     7021 aaaaaacacc taaagatttt cctacatcaa tagatgctat tttgtatgta atgaataatt
     7081 tagcacatat tactcaaaat ataacaccca tttttataat ggaattaaat aaatattatc
     7141 cacaattagc aataaaatta gataattttc gttatcgtca tatacgtgaa aaaattatgc
     7201 aaaatatagt aagaggtaaa aaagaaggtt tatatagaag tgatattaat gaagagatta
     7261 taagttattt ttatttagga tgggtaagtc aattttcaaa tgaaaacatg caaactgttt
     7321 ttgaaaataa atattcgaat caagaaatat tacacgagtt gtatcttttt catttaaatg
     7381 ctcttttaag tgaaaaaggt agaaaatatt tagaacaaaa attagaaaat tataataaag
     7441 atgaaaatgc taataacaac taaaaaattt tttttcagta ttattttgct gtcttcattt
     7501 atcttagtaa atgggcaaaa taataatgtg cagcttacct tacagcaagc tcacgattta
     7561 gctctacagc agaataaatc tattcaagct gcatctatgg ataaaattat tgctcaaaaa
     7621 actaaatggg aaactataac taattatttt cctaaagcca gtgctagtgc taaactttta
     7681 gacaatttaa aattacaaac tgtcttgtta ccagctgtaa tgataggagg agcgcctggt
     7741 gaatatattc ctgtacaatt tggtatggaa tttcaaacta gttggtccat agaagtgcag
     7801 caattgattt ttagcttgcc tttaattata ggtattcaat tatctaattt aggtgctgaa
     7861 atggctgaac tcggatattt gaatacagaa agagaagttg tatcttcagt caattctgtt
     7921 tatactagtg ctttagtatt aaagcataat ttgaatattc tagatagtaa tattgttaat
     7981 ttacaaaatg taaggcaaca aactcaagct atgtatgaag taggtgtatt gcaatcgaca
     8041 gatgtagatc agatagatct cacaattatg aatatagaaa atactaaaaa agcacttgag
     8101 cgtagcttag aactttctta taatgtacta cgttttcaat taggattaga cactaatgtg
     8161 gtttttgaac ttactactga tataaatgat atccttaatg aaggtgaaat aaataacctt
     8221 atactgacac ctgtagatat ttataaaaat atagattatc aatttgttga aaaacaaaga
     8281 gatatggctt tattaagttt aaggaaagaa aaatctgatt tcgctcctac gatagcaggt
     8341 ttctatacat atagtgaagc aggacaagga aataaactaa atgatttacg atggtttcca
     8401 gcatctattg cgggattgac gattagtttg ccgttaatta atggtggtca gaatggtgta
     8461 caagtgagta aggctaaagt agaactacaa aaatctaatc ttacactaga tatggtcaat
     8521 gagcaattaa aaatacaaga aaaacaactt aaatataatc tatctaatgc ttatgaaaat
     8581 tatcttacgc agaaaaaaaa tatcgaagta tcaaatagag tttataaaaa tatagaaatg
     8641 agatatctgc aaggcgtagc atctagccta gagcttactc aagctaatac gaattattta
     8701 actgctcagt caaattattt gagttctgta atgcagttat taaatgctaa atatgaacta
     8761 gagaaattgt tatataatca aaaatagtga aatatgaaaa taaaaaattt aattttagta
     8821 atagtattag gaactggtat attcctaaat gttagctgcg cttctaaaag tagtcaaaaa
     8881 gaaagcgaca gcgatgatat ttataatgtt caagtagcta aactcgaaaa acaaatttta
     8941 gataatactt tagagtttac agctaacatc gaagcatggg aaaaagtaaa tctagcacca
     9001 gcttctcctg gtcgtataat taaaatctat gctgaggttg gcaatcatat aaaaaaagga
     9061 caacttttag ttaatatgga tcctactcaa tacaatcaag ctcgtttgca attaactcaa
     9121 ttgcaaattg attataatag aatggattca cttgtaaaag taggagctat atctagacag
     9181 cagtatgatc aaataaaaac tcaatatgat atagccagaa ataatacaga atttctagag
     9241 caaaatgtta atctcaaagc tcctttctcc ggtgttatta caggaaaata tttcgaaaat
     9301 ggagaactat tttctggtgc tcctaatact aaagaaggta aagcagctat attaacttta
     9361 gaaaaaatga atgttttaaa agcttatgta gatatacctg agtcttatta taataacatt
     9421 aaattaaatt tacctgtaga aatcaaagta gatgcattag gagatcaaaa atttaatggc
     9481 aaagtttcac aaatttatcc tactgtagat ccatctagca ggacatttag agtagaaata
     9541 aaaatagata atccacgaga aattattaaa cctggtatgt ttgccagatt atctataaat
     9601 atgggtagga tagaagcatt agcagcccca tctattgctg ttttgaaatt gcaaggtact
     9661 aataaatatt atatttataa agtagaaaat aacatagcca aacaagtatt tatcgaacgt
     9721 ggtagaattt tagaaaacta cactgaaatc gctaatggca atattaaaga aggtgattat
     9781 atagttgtag taggacaaga taaattattt gatggagcaa agcttaatat cataaacaat
     9841 aaataataaa ttttttttat aataaaaagt aaagaactat gaaaatctat gaaacatcag
     9901 tcaaaaggcc aataactacg ttaatgattt ttttggctat aattgtattt ggcgtttatt
     9961 ctttctctag gttagcaatt gattttttcc cagagataga attacctact atttcaatta
    10021 tcacacccta cataggtgca gatgcggcca gtattgaaaa aaatattaca aaacctttag
    10081 aagatagatt aagtggtgta aatgatttag atgaaattta ttctacttct cgcgataatc
    10141 tttctattat cacactaaag tttatatatg gtacggattt gaatgaagcg gctaatgacg
    10201 caaggaatat catagatatg acaatgcggt ttttgcctga ggatgtagac agacctcaaa
    10261 ttttaaaatt ttctacaagt caaatgcctg tcattgtata tggtgtgcaa gctgatcaaa
    10321 attatgaagg attggataaa attattgaaa ataatataac caacaattta aatcgtatta
    10381 atggtgtggc atcagtattg gttagaggtg ctccagaaag agttgtttat gtagagtttg
    10441 atcctaaaaa attggaagct tataatatta ctcttgatca agtagctgca gttgtaagtc
    10501 gcgaaaatat cgatgtgccc gtaggtgata tcgaaacaaa agatatggaa tataatgtca
    10561 ctacagctgg agaatttatt tcgagtgatg aaataaaaaa tttaatagta ggttttcaaa
    10621 atggtaaatt tgttaaaatt agcgatatcg ctgtagtgag agatagcctt agagatcaaa
    10681 aaactatcga tcgtgtaaat gaaagtaggg gtgtgaatat tagcattatg aaacaatctg
    10741 atgctaatac tgtgcaggta tctcgaaaag taaaaaatga attagagaaa atcactaaaa
    10801 cacttcctgc tgatgttaaa attttcccta tctatgactc ttctgagaat attcaaaatt
    10861 ctattaataa tctatcagag actttactat ttgcttttat atttgtttca ttagtgatat
    10921 ttttattttt aggtaaatgg agttcatcat taattgtttt agttactatt cctgcatctt
    10981 taatctctgg atttatatat ttatttttag ccaaagatac attaaacatt atttctcttt
    11041 ctgctattac tattgctatt ggaatggtag tggatgatgc tattgtagta ttagaaaata
    11101 ttactaaaca tatagagaga ggtagcactc catacgaagc ttctatttat ggtactaatg
    11161 aggtatggac agcagtagta gcaagtactt tggttatttt agttgttttt atgcctctca
    11221 ctctgcttac tggaatcact ggtacaattt ttagaagttt aggttggata gtatctatta
    11281 ctattgcaat gtctattatt tgtgctatta ctttaacacc gatgcttagc tctagattat
    11341 taaaatctag ttcgttacaa taccaagata ataatggaaa aaagaaaaag aaaaatttat
    11401 ggcaatctac catgggagtg gtattagata aattagataa tgcttaccat aaatcattga
    11461 catatgtttt gaaacataaa acagccactc ttttgatagc tctagctatt tttgttggca
    11521 caatgtttct ggttcctatg ttagggtttg aatttctacc tgaagctgat caagatattt
    11581 tgagaggtac gatatatcta caaactggag gtaatataga taaaactgca gaatttgttt
    11641 ctaaattaga aaaaactgta aatgaaagat atccagaaaa atatatttta tcaatatctg
    11701 caggtgtgcc tagtgatcaa acagcctctc tcactggtac taaagctagt aatgaaatac
    11761 aagtaactct tagacttaaa aaacgtgaag aaagagaaag aacagtcttt gaaattgcag
    11821 aagattttag acaattttta gatgctagcc ctgaagtgat aaaatatgac attagtgcag
    11881 gtggaatggg aggtatgact aatactgttg atgttaagat ttttggttat aatatagata
    11941 gtagcttaaa agtagccagt gaattgcaat atcaaataag taaaattcca ggtgctagag
    12001 atgtaataat agatagagca aatgatagac ctacttttaa tatttatgtg gatagagaga
    12061 aagctgctaa actagggcta aatacagcta tgataggtag tgttgttaga aattatatta
    12121 ctggatatac tgcttctaaa tataaggaag aaggttatga atatgatgtg atatttagac
    12181 ttagtgacgt agctagaaaa gatgtagaga aatttaagaa tctaatgatc actactcctc
    12241 taggtactaa agtgtgttta tcagaagttg caaatattgt agaggaaact acaccacttg
    12301 agataagtca tgaaaataaa cagcgtgtta ttaccttaaa tgctaaacca gaaggtatat
    12361 ctcttggtga actaggaagt agtataaata agatcatcga taatgcatca tttcctgaag
    12421 gaataactgc agaagttggt ggaagttatg agaatttgac agattcgttt agagatttga
    12481 taacattatt atttgttgct atcttattag tttatattgt attagctgct caattcgaat
    12541 ctacgtctat gccattaata atactatttg cattgccttt tgctttttca ggtgtagttt
    12601 tagcattatt aataactggt aatacattga gtgtgaatgc tatgttaggt gctattatgc
    12661 tggtaggtat cgctgttaaa aatagcatta tccttgttga ctatactaat cttttacgag
    12721 atagaggtat gagattaaat gatgctgtag ttttgggtgg tagatctagg ttgcgtccaa
    12781 ttttgatgac tgctactaca acttttctag gtatgctacc tatggcattg agcacgggtg
    12841 aaggtagcga actatgggca ccaataggta tagctgttat tggtggatta gtgttttcta
    12901 ctatattatc actttttgta gtgcctgtgg cttatcatac ctttatacga tctggtagta
    12961 ggcgtaaaga gattatgcgc gctcgtcgtc aatttaaatt tattgatgaa attgttatta
    13021 ataataaaga cgataataat aataaataaa atttatatga aagctatttt tttaatacat
    13081 aatcaatcgg ttactgaaca aataaattat ttgttagaac ggttagatat taaagcttat
    13141 actaaatggg aaaccgtaat aggtaaaggt accgttggtg agcctcgttt tggtacccat
    13201 acatggccag agttaaactc ggctcttatt tctgtagttg atgatgaaaa agttcaacca
    13261 tttctttctg ctattcacaa attagataaa ataaatgagg gtgcgggtat taaagctttt
    13321 gtatgggatg ttttaatgag ctcagatgat gtggagtaat taattatctt taactgagaa
    13381 ctcataaact ccaaattaca aataaatctt tataactaaa atgtataaat tttgaatttg
    13441 tttgtaattt ggagttttat ttataagata tgtataaggc tttaagagat attatagtaa
    13501 acgttcattg atattgactc gtagagaata tagacaaaat cattaaggtt taattgtttg
    13561 gttttttatt atacccctat tatttttgat tagtaaaatt aactacagtt atcccatcac
    13621 cacctcgctc ttccacttca ttttcaaaat taagtaaatg tgtaagatct ttgagatagt
    13681 cccttacggc ttttcgtaga attccatagc cttttccatg tataatatat accctcggaa
    13741 caccaatcaa aagagcctgg tcgatatatt tgtctaattt attaatagct tcgtctgctc
    13801 tacatcctcg tatatctaaa acagtattaa aactttctgt acgactaacg agattaacct
    13861 ttattttttt attaatatat tgttcatttt tatcaatata atctactgca cttattggta
    13921 attgtatgat tttattattt actactacat ttaattggtt ttcagaaatt ctaataatct
    13981 caccaacctc tcctgttaat tttattttta ctaaatgtcc tacctcaaat tctatttttt
    14041 cttcttcttt aatagtatca gctacaagca ttttttctaa ttttttatca atagtatttt
    14101 tttccttttc taattttttt ctaatatctt ttactttatt tttctcagca ttactttctt
    14161 ttatttctct gattgtattt tcaattaatt tattggattt agaaaaaatc tcatctattt
    14221 ctccctttgt ttttttaata aagtaatttt gttttttctt ttgaaattct aaattatctt
    14281 ggtatttttt tattaattca gctacaaaat tttctgctag ttcaagttgt tttttttgtt
    14341 cttctaattt ttttctttca ttttctaaaa aaactagcaa tttttctgtt tcaattctat
    14401 tatctcctaa ttgattttgt gctttattaa taatatcttg agggaatcct actttttgag
    14461 caatttctaa tgtaaatgag ttgccaggta tgccttggga caaaatatat gtaggagaca
    14521 tagtatctgt atcgaaaagc aaggctgcat tttgcatagc aggataacgc ataggtagga
    14581 gtttgagatt atgaaaatgt gttgttacaa tagcaaaaca attattttga tatagtcgtt
    14641 ctaataccaa ttctgcaata gtgcctccca taataggttc agtgccggta cccatctcat
    14701 ccatcaaaat taaagattta tcattcgaat tttctaaaat atatttcata tttaataaat
    14761 gactagcata agtactgatc tcattttcta atgattgatt atctcccata tccataaata
    14821 tatgtgaaaa tatataaagg cgggaacctt catgagctgt gataggtata gcacattgaa
    14881 ccatccattg cattgtagct actgtcagta aaaccactga tttaccaccg gcatttggtc
    14941 cactaataat taaaaatcta ttattttggg ttaaatttat cgttaaaggt actatttttt
    15001 tgttatgtgt ggtaagtacg tgattcagat atggatgata agcatttatc agatttattt
    15061 ctggttcatc agttatttct ggtataattg cattattatc tgtactccac aaatattttg
    15121 cttttaatac atctaaataa gtgatctcat ctatgatgag taataaagtt tttatatatg
    15181 gcattaattg atcggtaaat tgtctgagaa tatggtaaat ttcttttttt tcatcaaaat
    15241 ataaatgttg taaagaagtg ttaagttcaa aaatttcttg tggttctata tatatggttt
    15301 gtccagtagc agattcatca tatataactc cctttacaga gtctttggct tcgggattta
    15361 ttggtattac taacctctga tcgcgaagcg taacattgga gtcatcagct acaaagcctt
    15421 ttttttgcca ttgcttccaa atctctttga tttgtttatc aatattacgc tgcatgctat
    15481 cgatgcgtcg tctgattgag gctaactcct cagaagcatc gtctctaata tatccttcgt
    15541 aagaccatat gtgctttaac tgttctagta tttgatcagc ttcattaaat ttaacatgct
    15601 gtgatctaag tattgacaat tttttatcat tgatgatttt atttaagtta atatatatag
    15661 tcaacagttg taatagcgaa tttaacattt cttggctaat aacaaagccc tcggtagcta
    15721 gtttttcgag ttcacttcgt atatcaccat ttattttagt agaaaaaatg ttgttttgtt
    15781 gaaaatattt ttttaaagat ttcagggctt catattcatt tattatcttt ttatgatcat
    15841 tagtcggtga taataaatca ataaaatctc taccatataa tgtttcacaa tacatagata
    15901 attgagattt tataaaatca aaataaattt tttgtggtaa atcttcagga taaaaaatca
    15961 ttgattaacc ctcctgtaat tagctaaatt acaaaaattt ttgcaaataa catgaaaata
    16021 aaaaagatac cgcaactaat aattgttggt tctataacgt ttattggggg taaattattt
    16081 aggttaattt tttttccaca aagagctatt tgtagccctt ttaatttcat ccttctctgt
    16141 gttctttgtg cattctcaga gaacacagag aaggatattt aacttttcta taataaaaaa
    16201 attaataaat gtttaagttt ttcaagttta tatgttaaat aatttttata tttttac
//
LOCUS       DAOPRN010000042        15854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_3115_length_15854_cov_97.901133, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000042 DAOPRN010000000
VERSION     DAOPRN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15854
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3115_length_15854_cov_97.901133"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PKI65_05780"
     CDS             <1..423
                     /locus_tag="PKI65_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HOB77985.1"
                     /translation="MVQAARSGKQNIAEGSMASGTSKETEIKLMGVARASLEELLLDY
                     KDYLRTNKLSLWGKDDPQALQIRALGKLKDRSYKTYRSYIEDSYPEVSVNTIICLIHQ
                     TNYLLDQQIRKLEEQFIKEGGIRENMMKARLNNKKNKN"
     gene            complement(754..1368)
                     /gene="pnuC"
                     /locus_tag="PKI65_05785"
     CDS             complement(754..1368)
                     /gene="pnuC"
                     /locus_tag="PKI65_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOB77986.1"
                     /translation="MFEYISVHWFEIIAASLGIIAIYFQINAKPFYWLISLVVSAMYI
                     VVYYTSKLYADMSMQFYYVGMSIYGLWAWLFGQKRNDNDERAPLPISTINNIKTWIII
                     AIITVGTFFLIGFILANYTDSNVPWWDAFTTALSFVATWMLARKKIENWIFWIVVDAT
                     SVALYIYKQLYPTTILFVLLTFLAVVGYIQWRKELKNMEIKKDI"
     gene            complement(1422..1685)
                     /locus_tag="PKI65_05790"
     CDS             complement(1422..1685)
                     /locus_tag="PKI65_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HOB77987.1"
                     /translation="MKTSVEISYYPLNEEYTKPIEKFLEALRSYKEIKIITNGMSTQI
                     FGEYDDVMKILTKEIKEAFQNPNSIFVMKIANACLDNYTLPSN"
     gene            1874..3181
                     /locus_tag="PKI65_05795"
     CDS             1874..3181
                     /locus_tag="PKI65_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOB77988.1"
                     /translation="MEAEIVHYNIPLWAIIPFVLMLLSIAICPLIIPEWWEKNKNKLL
                     VSLLLGIPTAIWMIAKGLSANLAHQLIFDYIPFIILLGSLFTITGGIHLKGDIEAKPT
                     NNLMFLLIGAVLASFMGTTGAAMLLIRPLITTISQRKYKTHTILFFIAIVANCGGLLT
                     PLGDPPLFLLYLRGAPFTWFLNLSKEWAFVNGMLLLMYFLADTYYYKKESPEDISFDK
                     TNKEPLKLVGKLNFIALLGVVASVAFLNPHYIPAMENNEYLGFIREGAMLLCALSSLY
                     FTPKQIRQDNFFTWHPILEVAFLFLGIFTTMTPTLMYLESNAASFGLSEPWHFYYATG
                     ALSAFLDNAPTAISFYQLAYGLASAHTGTLVAGISEQLLAAISLGAVFFGSMTYIANG
                     PNFMVKSIAEENKIKLPSFFGHMFKFSIIVLLPVYILTQLIFL"
     gene            3259..5760
                     /locus_tag="PKI65_05800"
     CDS             3259..5760
                     /locus_tag="PKI65_05800"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOB77989.1"
                     /translation="MKIDKKKLIPYGVALIVILGVIFIFLHPMLSGKIIAQPDIIQHA
                     GMSKEVNDYYKQTGEQAFWTSRLFSGMPTYLISMRYNDILVSKLDKVFTLFLHPHMGY
                     IFLLFVGFLFLMKVLKLDTWISIIAALAFTLSSYFIIILQVGHTSKLHAIGYMAPYLA
                     SVILIYQKKYKWGALLATLFLALNIRSNHLQITYYLAIITLFVAIYYTIIYIKKKDWK
                     HIGWATSIMVVTTIVGALLNFSLMYVTNEFSKETTRGKSELSIAENTKGLDKDYITQW
                     SYGVDETMTFLIPNFKGGVSDRIATVSPKSLTAADREWREAVASWEAYWGTQPFTSGP
                     VYIGAVVMLIFFVSLAFIWKNKLSIPLMLVTILAILLAWGRNFMPLTNFFIDYIPLYS
                     KFRTPSMILVIAELVIPIFFAFQVYDIYKNPDFYKKNLKKILIIGGVVSAIVLFIAVS
                     PSSFSSFLSPRDQEFINQYQAQGYNIDGFITSLVAVRAKMLSNDALRSLIFMLATLLV
                     IVLYIKKSIPKSAFLISLGLLVAIDLIPINKRYLNESHFITKKKYENPYPLTPADQYI
                     LADNKDGQGKVLNLTVNVFNDASTAYYHRDIGGYNAAKLKRYQELIEFHLSPEIQRFV
                     SIFQNQPLTMETIDSSLADLGTINMLNTKYIILQKDLMPLLNTHACGYAWNVNDVKLV
                     PNADAEISELDGIDPHNTLVIQDKYWDQKYNDALKSLDTNFQINITKFTPNEINYRYA
                     SSKPQIVAFSEVFYPEWEMQIDGQVQPIMKANYVLRAAYLPAGKHEIKMHFVPKIYNK
                     AKPITLTTNILFILLLIGAIVQEYLKYKKNSKISS"
     gene            5876..7501
                     /locus_tag="PKI65_05805"
     CDS             5876..7501
                     /locus_tag="PKI65_05805"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HOB77990.1"
                     /translation="MKRFFLFLTFTMISNLAISQISKLGTPWSFINKVTDINNIDTYV
                     MTPPDIDMLKADDELMYKFDKKIRIGVIQRVDIDANNYGSFDYLPNGDIIWRIKIYSK
                     DAKALSALFDYYKLPQGVKLYVYNPDKTFVLGAYTSEINNDENIVPIQSVPGEEMILE
                     FNIPSSIDDFSFFNLGEICYIYRDLGIDKASGSCNVNVACSEGANWVNQKNGVVKIRL
                     RAGSYYYLCSGSLVNNTKNDCTPYILTADHCGYESSTNDLNYWIFYFNYEAGTCNGTT
                     PIYYNHTVTGCTLVAHDNYGSTNSGSDFFLVKLKNNVPQNYNPYYNGWSRYTTPISTS
                     GVGIHHPDGDIKKISTYQNTLYSEYSTHWRVSWVATANGHGITEGGSSGSPLFNSSGK
                     IIGTLTSGTTSCDNLNGSDYYGKFSYHWASNGSTNDKKLQPWLDPLNQNPMELNGRDF
                     CVVNIDDYEILKNSVNIYPNPARDYINININNPDIKFFTIYIYDILGNLIFQSDINYN
                     DTYKYDVSYLEKGTYIIKITTDINSISLPFITQ"
     gene            7650..8402
                     /locus_tag="PKI65_05810"
     CDS             7650..8402
                     /locus_tag="PKI65_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOB77991.1"
                     /translation="MAKTKVLIVEDEAIIAKDIENVLTKAGYKVVGSFPTGEAAIAAV
                     EELNPDIILMDIMLKGDLSGIQAAEIIREKSNIPVVFLTAYADENTVDKARSAIPYGY
                     IVKPFKENELIATIEMALNKYKEDEDLKKERDMLKNMVIEKSDQDSIFVRADYRLNRV
                     RYSDIYYIEALKDYVVINTEDNIYTTHTTMKEMQRILPAKDFVRVHRSYIVRLDKIFS
                     IKYPDLVIEGKMKVIPIGGMYRKELFSRLNLV"
     gene            complement(8585..10645)
                     /locus_tag="PKI65_05815"
     CDS             complement(8585..10645)
                     /locus_tag="PKI65_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOB77992.1"
                     /translation="MIRPELINPKSIVIVGASNNVQKPGGKILKNILDHKYKGQIYIV
                     NPNSSEVQGFKSYQKVDDLPDQIDLAILAIPAHLCPDTVEILALQKGIKAFIIISAGF
                     SEESNEGAKLEKRIVDTINSVGGSLIGPNCTGVFNTNYAGIFTTPLPEINPQGCDFIT
                     ASGATGVFIVDLSMTRGLPFSSIYSVGNSAQLGVEDILEYMDETFDPDKSSKVKLLYM
                     ENVKKPQKLLKHAQSLISKGCRIAAIKAGYSEAGSRAAASHTGAMITPDIGVDALFKK
                     AGIVRCYGRDELVTVASIFMHKELIGNQLAIITHAGGPAVMLTDALSHSEIEIPPIKG
                     EKAQELLSYLFPGSSVNNPIDFLATGTAEQLGIIIDYCEKEFDNIDGMVVIFGSPGLF
                     PIFDVYNVLHEKMLTCKKPIYPILPCSINVADEVNDFISKGHVRFPDEVLFAKGLISV
                     YNTPKPQLHIKLPEIDKNTIRHIIDMSDNGFLAPHLVSQLLDAAGIPRAKEKVVTNEN
                     EIDIALKEIQYPLVMKVVGPIHKTDVGGVVLNVKNKEQLLKEFHRLMHIKDATGVLLQ
                     PMLTGTELFIGGKKEGEFGHIVTAGLGGIFVEVLKDIQIALSPVSLEEALNMIKNLKG
                     YKIIKGVRGSEGVDEQIFANIIQRVSALLITAPEISEMDINPLLGTGSNIVAVDARIN
                     IQKN"
     gene            10756..12765
                     /locus_tag="PKI65_05820"
     CDS             10756..12765
                     /locus_tag="PKI65_05820"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOB77993.1"
                     /translation="MIMNEFEKAILQGIPDKIPEMPVFEENINRAPKRRQILKEQEKK
                     LALKNALRYFSPKHHEFLAKEFLNELNEYGRIYMYRYMPTYSMYARNISEYPAKSKKA
                     AAIMLMIQNNLDPRVAQHPYELIVYGGNGSIFQNWAQYLLTMKYLATMTDEQTLVMYS
                     GHPMGLFPSSKDAPRVVVTNGLMVPNYSSMDDYDRYNALGVTQYGQMTAGSYMYIGPQ
                     GIVNGTTVTILNAGRKKEPGRQNLNGMLFVSSGLGGMSGAQPKAAKIAGGVAVIAEIN
                     PKPLKTRLNQGWLDEYYDSLDKLVPRLREAMVNKENVSIGYLGNIVDLWERLYDENIK
                     VDIGSDQTSLHNPYQGGYYPAGLTLEESNEMMSKNPELFKEKVHESLKRQVAIINKHA
                     ERGMYFFDYGNAFLLESSRAGADILLPNGEFKYPSYVQDIMGPLFFDFGFGPFRWICT
                     SGDPIDLDKTDKIAGDILEEMYKTAPDEIKLQLSDSINWIRNAKNNKLVVGSQARILY
                     ADGEARVKISKAFNEAIKSGYISAPIVLGRDHHDVSGTDSPFRETSNIYDGSKYTADM
                     ATHTFVGNAIRGATWVSLHNGGGVGWGEVINGGFGLVLDGSDEADKNLRSMLFWDVIN
                     GISRRSWARNPNAMVTLNRALSHHKDLSVTIPNLANDDLINKLFE"
     gene            13004..13420
                     /gene="ruvX"
                     /locus_tag="PKI65_05825"
     CDS             13004..13420
                     /gene="ruvX"
                     /locus_tag="PKI65_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOB77994.1"
                     /translation="MARILSIDYGAKRCGIAITDPMRVLAYPLTTVHKKDLLNFVIDY
                     CLQNEVDTIVIGEPINNNEKLSPIEIEIQGFINQLEKKLSTVDIVRIDESFTSKMAVR
                     VMIDAGAKKKQRQKKENIDKISAAIILQDYLKMLEL"
     gene            13417..13992
                     /gene="def"
                     /locus_tag="PKI65_05830"
     CDS             13417..13992
                     /gene="def"
                     /locus_tag="PKI65_05830"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOB77995.1"
                     /translation="MILPILAYNHPNLRIKAEPVGKDYPDLPQLLEDMWETLRKSDGV
                     GLAAPQINKSIKLFIVDARHYESKFPETKNFAKVFINPEIIKMSEETIYYPEGCLSVP
                     GIYEDVDRPKTITINYFDENWQEHTDTYTTIIARIIQHEYDHLDGILFPDRLPMVRKV
                     LIKKKLDNINKGNVNIDYPMIFSNKKKIKQS"
     gene            13995..14504
                     /locus_tag="PKI65_05835"
     CDS             13995..14504
                     /locus_tag="PKI65_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB77996.1"
                     /translation="MKKYIFSISIIILALLTSQCTQSKNKILEKINNLENEINASFPN
                     PNPLKIKELHKLYIEYANKYNEDTLSPHFIYKAAEAAVNIQEYDKAIDDLDLLINQYP
                     QHKLVPYAIFFKGFIYETFLNNKEEAERHYKTIIAKYKEHPLTKQAAFAIESLWMNDQ
                     EIAKILEIE"
     gene            complement(14625..15686)
                     /locus_tag="PKI65_05840"
     CDS             complement(14625..15686)
                     /locus_tag="PKI65_05840"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOB77997.1"
                     /translation="MKENLSFNVIGMMSGTSCDGVDLVLVKYFNNNNWQFDVLKTQTF
                     AYSHEWKNLLNNAFYLKGEEIINLNFRYSHYLSDLILYFLSTINTDVDFISMHGHTIW
                     HNPAAGYTYQLGHGGVIASDTSIPVICDFRSQDVGLGGEGAPLVPIGEKYLFTDYNIF
                     LNLGGFSNATIIENGEIISAFDICPVNIILNYLTQKINLSYDKNGELARNGLIIDDLL
                     TSLLTINDTFDNHSLCREIVEKEFIPLINKYADQFTINDLLRTFVEYVTVTISSKLPQ
                     NGRVLVSGGGAHNNFLIKKLSENYEGEIILPSREIIDYKEAIIFAFLGLLRWIGLPNT
                     STKVTHSAKESCSGAIYLP"
BASE COUNT         5669 a         2404 c         2436 g         5345 t
ORIGIN      
        1 atggtgcagg cagcaaggag tggaaaacaa aatattgcag aaggcagcat ggcatctggc
       61 acttctaaag aaactgaaat aaaattaatg ggcgtcgcta gagctagcct agaagagcta
      121 ctattagact ataaagatta tttaagaaca aataagcttt cactttgggg gaaagatgat
      181 cctcaagcac tacaaataag agctctggga aaattaaaag ataggtcata taagacttat
      241 aggtcatata ttgaagattc ttacccagag gtttcagtaa atactattat ctgtttaatt
      301 caccaaacaa attatctatt agatcaacaa ataagaaaat tagaggaaca atttataaaa
      361 gaaggtggaa taagagaaaa tatgatgaaa gccagattaa ataataaaaa aaataaaaat
      421 taattaaaca ctcgaataat caaaggatgg gctttcatta ggcaattcat gccccaataa
      481 cgaattgctt taacgattcg ctcaaacaaa tcgcatcaat aaaaacacct catatgaaca
      541 tataatcata tgatctatta accacgtcct ttaggtcgag gaacaacaaa gaaaaaggaa
      601 tgggctttag ccctgaaata ttctgaataa tggatgcgat aataaataat aaaaaaacct
      661 tttatgggta attcacaaat ctacccaatt aatcgtccca acgaaaaaca cctgtatatt
      721 taatgattgt aaaattatat ttggtcaaat gcttcaaata tcttttttga tttccatatt
      781 tttcaattct tttctccatt gtatataacc gacaactgct aaaaaagtaa gtaaaacaaa
      841 aagaatcgta gtgggataaa gttgtttata aatatagagt gccacactgg tagcatctac
      901 gacgatccaa aatatccaat tttctatttt tttgcgagca agcatccacg tagctacgaa
      961 actaagtgca gtagtgaatg catcccacca agggacattg ctatcggtat aattagctaa
     1021 aataaatcca attaaaaaaa atgttcctac tgttatgata gcaataataa tccatgtttt
     1081 tatattatta atagtagaaa ttggcaatgg tgccctttca tcattatcat ttctcttttg
     1141 tccgaagagc cacgcccaaa gcccataaat ggacattcct acataataaa attgcatact
     1201 catatcggca tatagcttac ttgtataata tactactatg tacatagctg atactactaa
     1261 actgattagc caataaaacg gtttggcatt aatctgaaaa tatatagcta ttatacctaa
     1321 gctagctgct attatctcaa accaatgtac acttatatat tcaaacaaat catatatagt
     1381 tttttaaaaa aaaactcgtt acaccaaaaa taaaatttta tttaatttga aggtagagta
     1441 taattatcta aacaagcatt tgctattttc ataacaaaga tagaattagg attttggaac
     1501 gcttctttta tctctttagt caatattttc attacatcat catactcacc aaatatctga
     1561 gtgctcattc catttgtgat tatttttatt tctttataag atctcaatgc ctctaaaaat
     1621 ttttcaatag gctttgtgta ttcctcattt aatggataat aactaatctc tactgaagtt
     1681 ttcatttttt tttacaaata tatgaataaa ttataaatca taatctacaa actagcaact
     1741 gataatacct gatcatactt aaaaattcct aatctcaaaa tccttaatta ataaaaaaat
     1801 aatgcaattt tttttgatta attataaaca aattcaactt tttgattaat tttgctatca
     1861 aaaaaaaact attatggaag cagaaatagt acattacaac attccgttat gggccattat
     1921 accatttgta ttaatgcttt tatctatagc tatatgccca ttaataattc ccgagtggtg
     1981 ggaaaaaaat aaaaacaaac tgttagtatc gttactatta ggtataccta cagccatatg
     2041 gatgatagca aaaggacttt cagctaatct agcacatcag ttaatatttg actatattcc
     2101 atttattata ctcttaggtt cattatttac aataacagga ggtatacatt taaaaggcga
     2161 catagaagct aaacctacta acaatctaat gtttctcctt atcggagcgg tgttagcatc
     2221 attcatgggt actacaggtg ccgcaatgct gctgataagg ccattaataa ctaccatttc
     2281 acaaagaaaa tataaaacgc atacaatatt attttttata gctatagtag ctaattgtgg
     2341 tggcttactt acgcccttag gtgatccacc cttattttta ctctatttgc gtggtgcacc
     2401 atttacatgg tttttaaatt tatcaaaaga atgggcattt gtaaatggta tgctactact
     2461 tatgtacttt ttagcagaca catattatta taaaaaagaa tctccagaag atatttcatt
     2521 tgacaaaaca aataaagaac cattaaaatt agtaggcaaa ctaaatttca tagcattatt
     2581 aggcgtcgta gcttcagtag catttttaaa tccacattat atacctgcaa tggaaaataa
     2641 tgaatattta ggctttattc gtgaaggggc tatgctttta tgtgctctat catcattata
     2701 tttcacacct aaacaaatca gacaagacaa ttttttcact tggcatccaa tattagaagt
     2761 tgcattttta tttttaggta ttttcactac tatgacacct actttgatgt atttagagtc
     2821 taatgctgca tcatttggat tgtctgagcc atggcatttt tattatgcta ctggggcact
     2881 cagtgctttt ttagataatg ctcctactgc tatatctttt tatcagcttg cttatggatt
     2941 agctagtgca catactggta ctttggtagc aggtatatcc gaacaactat tagctgctat
     3001 ttctttaggg gctgtttttt ttggatccat gacatatata gctaatggac caaactttat
     3061 ggtaaaatct attgccgaag agaataaaat caaattgcct agtttctttg gacatatgtt
     3121 taaattttca ataatcgttt tattacctgt ctacatatta actcaattga tatttttata
     3181 aaaatatcat aaaattactt tactaaaaaa agctaatatt aatttttcta ttaaatttgc
     3241 ttagaaaaat atattaatat gaaaatcgac aaaaagaaat taatacctta tggggtagca
     3301 ttaatagtga tattaggggt tatatttatt tttttacatc ccatgctttc tggcaaaatc
     3361 atagctcaac cagatataat acaacatgca ggcatgtcta aagaagttaa tgattattac
     3421 aagcaaaccg gcgaacaagc tttttggaca tcacgtctct ttagtggtat gcctacttat
     3481 ctgatatcta tgcgatataa tgatatttta gtttctaaat tagacaaggt ttttacactt
     3541 tttttgcatc cacacatggg atatatattt cttttgtttg ttggcttttt atttttgatg
     3601 aaagtattga aattagatac ttggatctcg ataattgcag ctttagcatt cactttatct
     3661 tcatatttta taataatctt gcaggtaggt catacatcta agctacatgc tataggctat
     3721 atggctccat acctagcctc tgtcattttg atttatcaaa aaaagtataa atggggagcc
     3781 ttattggcaa cattattttt agcattgaat attagaagca atcatttaca aattacatat
     3841 tatttggcta ttattactct tttcgttgct atatattata ctattattta tattaagaaa
     3901 aaagattgga aacatatagg ttgggctact tctataatgg tggttacaac catagtagga
     3961 gcgttactta attttagttt aatgtatgta actaatgaat ttagcaaaga aactacacga
     4021 ggcaaatctg agctctcgat tgcagagaat acaaaaggtt tagacaaaga ctacattaca
     4081 caatggagtt atggtgtaga tgaaacaatg acatttttga tacccaattt taaaggtgga
     4141 gtaagtgata gaatagctac agtatcacct aaatcactca cagcagctga tcgcgagtgg
     4201 agagaagccg tagctagttg ggaagcttac tgggggacac aaccatttac ctctggtcct
     4261 gtatacattg gtgctgtagt gatgcttatc ttctttgtta gcctagcttt tatttggaaa
     4321 aataaactta gcataccatt aatgttggtt actattttgg ctatcttgct agcatgggga
     4381 aggaatttca tgcctttaac aaactttttc atcgattata ttccactata tagtaaattt
     4441 cgtactccgt ctatgatact cgtaatagct gaattagtaa ttcctatatt ttttgctttt
     4501 caggtatatg atatctacaa aaaccctgat ttttataaaa aaaatcttaa aaaaatattg
     4561 ataataggag gtgtagtctc tgcaatagtg ctttttatcg cagtgagtcc atctagtttt
     4621 tcgagctttt tatcacccag agatcaagaa tttatcaatc aatatcaagc tcaaggttat
     4681 aatatagatg gattcatcac ctcattagta gctgtcaggg ccaagatgct tagcaatgat
     4741 gctttacgtt cattgatttt tatgttagct actttgcttg ttatagtttt atatattaaa
     4801 aaatctattc ctaagtctgc ttttttaatt agtttaggac tactagtagc tatagatcta
     4861 ataccaataa ataaaagata tctcaatgag tctcatttca ttactaagaa aaaatatgaa
     4921 aatccttatc cattgactcc agcagatcaa tatatattag ctgataataa agatggtcaa
     4981 ggcaaagtgc taaatctgac tgttaatgtt ttcaatgatg cgtccactgc ttattatcat
     5041 cgcgatatag gtggatataa tgctgctaag ctaaaaagat atcaagaatt aatagaattt
     5101 catctatcac cagaaataca aagatttgtt tctatttttc aaaatcaacc tcttacaatg
     5161 gaaacgatag atagcagttt ggccgatctt ggaaccatca atatgctaaa tactaaatat
     5221 ataatattac aaaaagatct gatgccacta cttaatacac acgcttgtgg atatgcttgg
     5281 aatgtaaacg acgttaaatt agtccctaat gcagatgcag aaatctctga attagatggc
     5341 attgatccac acaataccct tgtaattcaa gataaatatt gggatcaaaa atataatgat
     5401 gctcttaaat ctttagatac taattttcag attaatataa ctaaatttac acctaatgag
     5461 atcaattatc gttatgcctc atctaaacct cagatagtag ctttcagtga ggttttttat
     5521 ccagagtggg aaatgcagat agatggacaa gtgcaaccaa ttatgaaagc taattatgtc
     5581 ttgcgtgctg cttatctgcc cgctggcaag catgagataa agatgcattt cgtccctaaa
     5641 atttataata aagctaaacc tatcactttg actactaata ttttatttat attactacta
     5701 ataggtgcta tagtgcaaga atatctaaaa tacaaaaaaa attctaagat ctcttcatag
     5761 agagtaatat tttgttttgc ttaaaataaa ttttttaata aattagttaa ttgattttat
     5821 taaaataaaa ttaattaact ttggcatcct aaatcactta aaaaatttaa aaaacatgaa
     5881 acgatttttt ttatttttaa catttactat gatttctaat cttgcaatta gtcaaattag
     5941 taaacttggt accccatgga gttttatcaa taaagtaaca gacatcaata atatagatac
     6001 ttacgttatg acacctccag atatagatat gctaaaagct gatgatgagc ttatgtataa
     6061 atttgataaa aaaataagaa tcggtgttat acaaagggta gatatagacg caaataatta
     6121 tggtagcttc gattatttac ctaatggaga tatcatttgg agaataaaga tatattctaa
     6181 ggatgctaaa gcactctcag ccttatttga ttattataaa ctaccgcaag gtgtcaaatt
     6241 gtacgtatat aatccagata aaacttttgt tttgggcgca tacactagtg aaatcaataa
     6301 tgacgaaaat atagttccta tacaatctgt gcccggcgaa gagatgatat tagaattcaa
     6361 tattcctagt agtatagatg atttttcttt tttcaattta ggagagattt gttatatata
     6421 tagagatctg gggatagata aagcatcagg atcctgtaat gtaaatgtag cttgttctga
     6481 aggtgctaat tgggtaaatc agaaaaatgg agtagttaaa ataagattac gcgctggtag
     6541 ttattattat ttatgttcag gttcattagt caataatacc aaaaatgatt gtactcctta
     6601 tattttaaca gctgatcatt gcggctatga atctagtaca aatgatttga actattggat
     6661 attttatttt aactatgaag ctggcacatg caatggtact acccccatat actataatca
     6721 tacagttaca ggatgcacat tagtagctca tgataattat ggctcaacta atagtggttc
     6781 agattttttt ctagttaaat taaaaaataa tgtaccacaa aattataatc catattataa
     6841 tggttggtct agatatacta ctcctatatc tacttcgggc gtaggtatac atcacccaga
     6901 tggtgatata aagaagatca gtacctacca aaatacacta tatagtgaat atagtactca
     6961 ttggagagtt agttgggtag cgactgctaa tggacatggt attactgaag gtggatctag
     7021 tggttcacct ctatttaata gtagtggaaa aatcattggt actctaacaa gcggtactac
     7081 aagttgtgat aacttgaatg gaagtgatta ttatggtaaa ttttcttacc attgggcttc
     7141 taatggatct actaatgaca aaaaattaca accttggctc gatcctttaa atcaaaatcc
     7201 tatggagctc aatggtagag acttttgtgt agttaatatt gatgattatg aaattctaaa
     7261 aaattctgtt aatatttatc caaatccagc tagagactac attaatatca atatcaataa
     7321 tccagacatt aagtttttta ctatttatat atatgatatt ttaggtaatc taatttttca
     7381 gtctgatata aattataatg atacttataa atatgatgtt tcttatttag aaaaaggaac
     7441 ttatattatt aaaattacaa ctgatataaa ttcaatttca ttacctttta ttacgcaata
     7501 aatactattt agtataaaat atcacttgat ttagctacaa atacacataa ttcagctaat
     7561 ataatatgtg atttatctat taattgtatt aatttttgat ttacatatat attatttgta
     7621 attttgtcga aaaataaaat aaaaaaatta tggcaaaaac aaaagtttta attgtagaag
     7681 acgaagctat cattgcaaaa gacattgaaa atgttttgac aaaagctggt tataaagttg
     7741 taggttcctt tcctacaggt gaagcagcta tagctgctgt agaagaatta aatccagata
     7801 ttattttgat ggatataatg ttaaaaggag acttatctgg tattcaagct gctgagataa
     7861 ttcgtgaaaa atctaacatt ccagtagttt ttctcacagc ctatgcagat gaaaatactg
     7921 tagataaagc tagatctgct attccttatg gttacatcgt aaagcctttc aaagaaaatg
     7981 aactaatcgc tactatcgaa atggctctca ataaatacaa agaagatgaa gatctgaaaa
     8041 aagaaaggga tatgctaaag aacatggtca tagaaaaaag tgaccaagat agtatctttg
     8101 taagagcaga ttacagatta aatagagtga gatattctga tatatactat atagaagctt
     8161 taaaagatta tgtagttatt aatactgaag acaatatcta cactactcac actactatga
     8221 aagaaatgca aagaatacta ccagctaaag attttgtgcg tgttcacaga agttatattg
     8281 ttcgtttaga caaaattttt agtattaaat accctgactt agtaatcgaa ggcaaaatga
     8341 aagtgattcc tatcggtggt atgtatcgca aagaattatt cagcagacta aatttagttt
     8401 aatattattt tttatgaact aaaaaacgcc tattatcaaa tgataatagg cgtttttgtt
     8461 atatattttg ctataaatat ataataaaaa ctccctaata tttctattac cataaaaata
     8521 ttaacatact taattatctt tatttattgc taaaaatttc gaatatcaaa atcagtttta
     8581 aagtttaatt tttttggata tttatccttg catctacagc tactatatta gaaccagttc
     8641 ctagaagagg gttaatatcc atttcagata tttctggagc agttatcagc agagcactaa
     8701 cacgttgtat tatattagca aaaatttgct catctacacc ttcactacct ctaactcctt
     8761 ttattatttt atatcctttt agatttttta tcatattcaa tgcttcttct aaagatactg
     8821 gcgatagagc aatttgtata tcttttaata cttctacaaa tatgccaccg agtcctgctg
     8881 tgactatatg tccaaactca ccttcttttt taccacctat aaacaattca gtaccagtaa
     8941 gcattggttg caatagtact cctgtagcat ctttgatgtg cattaatcta tgaaattctt
     9001 tcaataattg ttctttattt ttaacattca ataccacacc acccacatca gttttatgta
     9061 tagggcctac taccttcatt actagaggat attgtatttc ttttagagct atatctattt
     9121 cattttcatt agttaccact ttttcttttg ctcgtggtat accagcggca tctaagagtt
     9181 gcgacaccaa atgtggtgct aggaatccat tatcactcat atcaataata tgacgaatag
     9241 tgtttttatc tatttctggt aattttatat gaagttgtgg ttttggagta ttgtatactg
     9301 atattagtcc tttagcgaaa agaacttcat cagggaaacg tacgtgtcct tttgagataa
     9361 aatcattcac ttcatcggct acattaatag agcatggtaa tataggatat atgggttttt
     9421 tacaagtaag cattttttca tgaagtacat tataaacatc aaaaatcgga aacagtcctg
     9481 gactaccaaa tattactacc atcccatcaa tattatcaaa ttccttttcg caataatcta
     9541 ttatgatacc taattgctca gcagtacccg ttgctaaaaa gtctattgga ttattcactg
     9601 atgaaccagg aaaaagatat gaaaggagtt cttgagcttt ttcgcctttg atggggggaa
     9661 tttctatttc actatgagaa agtgcatcag ttagcataac ggcaggtcca cctgcgtgag
     9721 taattatagc taattgattt cctatcaatt ctttatgcat aaaaatagag gctacagtta
     9781 ccagttcatc gcgaccatag caacgtacta ttcctgcttt tttgaataat gcatctacac
     9841 ctatgtcagg agtgatcatt gctccagtgt gtgaagcagc tgctctagag ccagcttcag
     9901 aataaccagc cttgatagcg gctattctgc aacctttaga tatgagagat tgagcatgtt
     9961 ttaataattt ttgtggtttt ttaacatttt ccatgtacag caatttgact ttagaacttt
    10021 tatcgggatc gaaagtctca tccatatatt ctaaaatatc ctctacacct aattgagcac
    10081 tattgcctac tgaataaata cttgaaaatg gcaagcctct agtcatacta agatctacta
    10141 taaatacacc tgtggcccca ctagctgtta taaaatcaca accttgtgga ttaatttctg
    10201 gtaatggcgt agtgaatata cctgcgtaat tagtattgaa tacgccggta caattagggc
    10261 ctattaggct gccacctact gagtttatag tgtctacaat tcttttttct aacttagcac
    10321 cttcattgct ctcttcgctg aaaccagccg aaatgataat aaaagcttta atgccttttt
    10381 gaagagctaa gatttctact gtatcagggc acagatgtgc aggtattgct aggatagcta
    10441 gatctatctg gtcaggtaaa tcatctacct tttgatatga tttgaaccct tgcacttcgc
    10501 tactatttgg attgacaata taaatctgtc ctttatattt gtggtctaaa atatttttta
    10561 aaatcttccc acctggtttt tgaacattgt tagatgcacc tactattact atacttttag
    10621 gattaattaa ttctggcctt atcataaatt attaattttt aggcaaaaat actaaaaatg
    10681 atttgaaaaa ttaaaaatag ttattatatt ataaattttt taagaaaaaa ttattaaatt
    10741 cgcaaaaaat aattcataat tatgaatgaa ttcgaaaaag ccatcttgca aggtatacct
    10801 gataaaatac ctgaaatgcc agtatttgaa gaaaatataa atagagctcc taaacgtaga
    10861 caaatattaa aagaacaaga gaagaaattg gcactcaaaa atgctttacg ttatttttca
    10921 ccaaaacatc atgaatttct agccaaagaa ttcttaaatg agttaaatga atatggtagg
    10981 atttacatgt ataggtatat gcctacatat tctatgtatg ctagaaatat aagtgaatat
    11041 ccagctaaat ctaaaaaagc agctgccatc atgctaatga tccaaaacaa tctagatcca
    11101 cgagtagcac agcatcctta tgaactgata gtatatggtg gtaatggatc tatttttcaa
    11161 aactgggctc agtatttatt gacaatgaaa tatttagcta caatgacaga tgagcaaact
    11221 ttagttatgt atagtggcca tcctatggga ctgtttccga gttcaaaaga tgcacctcga
    11281 gtggtagtaa ctaatggact aatggtaccc aactatagca gtatggatga ctatgacaga
    11341 tataatgctc taggagtaac acaatatggg cagatgacag ctggatcata tatgtatata
    11401 ggtccacaag gaatagtgaa tggcactact gtaactattt tgaatgctgg taggaaaaaa
    11461 gaacctggca gacaaaatct caatggaatg ctattcgtat cttcaggtct tggtggtatg
    11521 tctggcgcac aaccaaaagc tgcaaaaata gctggtggtg tagctgtaat agcagaaatc
    11581 aatcccaaac cactgaaaac aagattaaat caaggttggc tagatgaata ttatgattcc
    11641 ctagacaaac ttgtacctag actaagagag gcaatggtaa ataaagaaaa tgtttctatt
    11701 ggttacttgg gtaatattgt agatttatgg gaaagattat atgatgagaa tataaaagta
    11761 gatataggct cagatcaaac ttcattgcat aatccatatc aaggtggtta ctatcctgca
    11821 ggacttaccc ttgaagagtc aaatgaaatg atgagcaaaa atccagaact tttcaaagaa
    11881 aaagtacatg aaagtctaaa acgtcaagtt gctattatca ataaacatgc agaacgtggt
    11941 atgtactttt ttgattatgg taatgcattt ttattagaat ctagtcgtgc aggtgcagat
    12001 attttattgc ccaatggtga atttaagtat ccgagctatg tacaggatat aatggggcct
    12061 ttattttttg attttggctt cggtccattc agatggattt gtacatctgg cgatcccatc
    12121 gatttagata aaacagataa aatagctgga gatatattag aagaaatgta taaaacagct
    12181 ccagatgaga taaaactaca attaagtgat agcattaatt ggattagaaa cgctaaaaat
    12241 aataaattag tagtaggtag tcaagccaga atattgtatg ctgatggtga agctagagtg
    12301 aaaatctcaa aagcctttaa tgaagctata aaatctggat atataagtgc tccgatagta
    12361 ttaggtagag atcatcacga tgtatctggc acagattcac catttagaga aacttctaat
    12421 atttatgatg gtagtaaata tacagctgat atggctactc atacttttgt aggtaatgct
    12481 atcagaggtg ctacttgggt atctctgcat aatggcggtg gtgtaggttg gggcgaagtg
    12541 atcaatggag gctttggact agtactcgat ggtagcgatg aggcagataa aaatttgaga
    12601 agcatgctct tttgggatgt gataaatggg atctctcgta gaagctgggc tcgcaatcct
    12661 aatgctatgg ttactctcaa tagagcccta tcacatcata aagatttatc tgttacaata
    12721 cctaatttag ctaatgatga tttaattaat aaactttttg aataacatct cattattaaa
    12781 aaaatataat gaagtttaaa aaatatttat tttacttttt gcttattatt tctatatttc
    12841 ttttatcctc ttgtcaaacg caaaaaaaac ctctgcctaa taatattaag aaatataatc
    12901 ctaaaaaatg taatacttgt cccggattta attaataata ggtttaatag acaaaattat
    12961 ataataaaaa ttcattaaat ttgcatacaa attttaaaaa aaaatggcac gtattttatc
    13021 aatagattat ggagccaaac gttgtgggat agctattaca gatcctatgc gagtattagc
    13081 gtatccactg actacagtgc ataaaaaaga tttattaaac ttcgtcatag attattgttt
    13141 gcaaaatgaa gttgatacaa tagtaatagg agagcctatc aataataatg aaaaattgtc
    13201 acctatcgaa atcgaaattc aagggtttat aaatcaatta gaaaaaaaat tatcaacagt
    13261 agatatagtg agaatagatg aaagtttcac atctaaaatg gcagtaagag taatgataga
    13321 tgcaggagcc aaaaaaaagc aaaggcaaaa aaaagaaaat attgataaaa taagtgcagc
    13381 tatcatatta caagattatt taaaaatgtt agaattatga ttttacctat tttagcttat
    13441 aatcatccaa atttgcggat aaaagcagaa cctgtaggca aagattatcc agatttacca
    13501 caactcttag aagatatgtg ggaaacatta agaaaaagtg atggtgtagg gttagctgct
    13561 ccacaaataa ataaatctat taagcttttt atagtagatg ctaggcatta tgaaagtaaa
    13621 ttccccgaga ctaaaaattt tgcaaaagta tttatcaatc ctgaaattat aaaaatgtcg
    13681 gaagagacta tatactatcc agaaggatgc ctaagtgtac caggaattta tgaagatgta
    13741 gatcgaccaa aaacaataac tattaattat tttgatgaaa attggcaaga acatacagat
    13801 acttacacaa ccattattgc tagaattatc caacatgaat atgatcactt agatggtatt
    13861 cttttcccag atagattgcc aatggtaaga aaagttttga tcaaaaagaa attagataat
    13921 attaataaag gtaatgtaaa tatagactac ccaatgattt tctcaaataa gaaaaaaata
    13981 aaacaaagtt gattatgaaa aaatatatat tttcaatatc tatcattata ttagctctat
    14041 taactagtca atgtactcag agcaaaaata aaatattaga aaaaattaat aatctagaaa
    14101 atgaaataaa tgctagtttt cctaatccca atccattgaa aatcaaagaa ttacataaat
    14161 tatatataga atatgctaat aaatataatg aagatacttt atcaccacat tttatatata
    14221 aagcggctga agctgctgta aatattcaag aatatgacaa agctattgat gatttagatt
    14281 tacttataaa tcagtatcca caacataaac tcgttcccta tgctatattt tttaaaggtt
    14341 ttatatatga gactttttta aacaacaaag aagaagcaga aagacactat aaaactatta
    14401 tcgccaaata taaagaacac ccattaacaa aacaagctgc ttttgcaata gaatcattat
    14461 ggatgaatga tcaagaaata gcaaagattc tagaaataga ataattgata caaaaattac
    14521 taataagtag tatgtaaaag aaaaattcat tataaaatca taagttacta aaaattaaaa
    14581 aataataatt tttattattt taaacttatt ttagaaatat tttttcaagg caaataaata
    14641 gcaccactac atgactcttt agcagaatga gttacttttg tactagtatt tggtaatcca
    14701 atccatcgca acaatcctag aaaagcaaat attatagctt ctttataatc tataatttct
    14761 ctactaggta aaattatttc gccttcataa ttttcactta atttttttat caaaaaatta
    14821 ttatgtgcac cgccacctga tactaatact ctaccatttt ggggtaattt acttgaaatt
    14881 gttactgtaa cgtattctac aaatgttcgt aataagtcat taatggtaaa ttgatctgca
    14941 tatttattta ttaaaggaat aaactctttt tctactattt ctctgcaaag tgaatgatta
    15001 tcaaaagtat cattaattgt aagtagacta gttagtaaat catctataat tagaccattt
    15061 cgagctaatt caccgttttt atcataagat aaattaattt tttgagttaa ataatttaaa
    15121 attatattta ctggacaaat gtcaaaagct gaaattattt ctccattttc tattattgtg
    15181 gcattactga aaccacctag attcaaaaat atgttataat ctgtaaacaa gtatttttct
    15241 ccgattggta ctaatggagc gccttctcca cctaatccaa catcttggct acgaaaatcg
    15301 caaattactg gtatagatgt atcgctagct atcactccac catgtccgag ctgatatgtg
    15361 taacctgctg ctggattgtg ccatatcgta tgtccatgca tgctaataaa atctacatct
    15421 gtattaattg tagacaaaaa atatagaatt aaatctgata aatagtgaga gtatcgaaaa
    15481 tttagattta taatttcttc accttttagg tagaaagcat tatttagcaa atttttccat
    15541 tcgtgtgagt aggcaaaagt ttgcgttttg agcacatcaa attgccaatt attattgtta
    15601 aaatatttta ctagcactag atctacaccg tcgcagctag taccagacat catacctatt
    15661 acattaaaac ttagattttc tttcataata atggcaatat tttttctaga gcatttgccc
    15721 tggagccctt tactagtact aggtattcat ttaatggatt atttagaata aaatttatta
    15781 attctttagt attaggtaaa aattgaaatt gtggaaaatt ttctttgaac tgataaaaat
    15841 tttctcctac aaat
//
LOCUS       DAOPRN010000043        15570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_3186_length_15570_cov_117.212311, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000043 DAOPRN010000000
VERSION     DAOPRN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15570
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3186_length_15570_cov_117.212311"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PKI65_05845"
     CDS             complement(<1..901)
                     /locus_tag="PKI65_05845"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB77998.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRTGNLLFKKFESKKLSPEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEIVTSNRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGK"
     gene            complement(1203..2261)
                     /locus_tag="PKI65_05850"
     CDS             complement(1203..2261)
                     /locus_tag="PKI65_05850"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOB77999.1"
                     /translation="MRTLVNEWLPVVFNAIQITSFVIVIMLLIEYLNIKTKGKFFKGI
                     KSKSWKSVLTGSLLGILPGCMGSFFVVSLFTHGEITIGALLANFIATTGDESFAMLGL
                     MPIKAILIFLFLFVISFISGILIDKITKNHTLSTDNQHFVIHQHDYENLKYSSIKENI
                     KHLSLSRALLLFLLILFIIIILLPNKEHGHHEGAFSLEIILNYLLVIVALLMIYPIIK
                     LPDHFIEEHMWGHVIKKHFLRLFFWILLGLIAVKLINKNVNVEYWIHDNIYLTILISI
                     LIGLIPASGPHFIFISMYIGGIVPISVLLANNITQDGHAGLPLLAESWKTFLLIKGIK
                     IIIAFTIAVIIQLIFGWI"
     gene            complement(2242..2898)
                     /locus_tag="PKI65_05855"
     CDS             complement(2242..2898)
                     /locus_tag="PKI65_05855"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOB78000.1"
                     /translation="MKKIIIYLLTIIIIIIIYLSALEVKANKKSITSTYNITSPQVDK
                     IQSADIILRLGYGVISESIAKLLNEEYKISHCGIIVPCDSCLYNYKVIHSVSSSLSPI
                     NGVQDDCLEHFIRNSQPNSIIITRYKNLDSLDILNLIYWSNYFLDRQIPFDHNFDIVD
                     SSSFYCSELIHYIFKKANVTLLYDNISNHNLDNLKFKIFWDDNNFEIIINHQNENFSK
                     "
     gene            3195..5294
                     /locus_tag="PKI65_05860"
     CDS             3195..5294
                     /locus_tag="PKI65_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOB78001.1"
                     /translation="MQSKSIFITFILIFITSFFVYGQKNLVEEADEKFELNQFYSASQ
                     LYLKAYPKVDNKLEKNRILYQVGLCYYNMGDARKALSYFNRVIRAQYPDPEVFWYTGL
                     VNMTLANYEEALNNFEKYKGLVPNDTLVDIKIESCKLSKAWMDNPTLYEVTSERKLNT
                     RNQDFATAWADKRYRSIIFTSNRDGVVGKGIDEWTGLPFTDFFIAEKDKKGNWSSPVT
                     ADDQEILNTQHNEGVGCFNNKFNTFYFTRCRVEKKKILGCNIYYTTRRGRSWAEPILL
                     PFTTDSTATVGHPAISDDEMMIIFSGNLSGGYGGRDLWMATRTKKNQDFGEPSNLGPI
                     INTSGNEMFPTLRYVNNRTYLYFSSDGHPGMGGLDLYRSEYIDGQWTVPENLRYPLNS
                     PQDDFHIIFLDDPTILRTEQAREMGFLSSNRNLSSMDDIYSFKLPLIYFSISGIVYDD
                     STKKPIPGAIVRIEGSDGNIYIDTTDNNGYYIFDKTQIFENNTYTIYVSKEEYLGERG
                     IETTVGLDRSKDLVHDFYLIPIPKEPIVLPDILYDFDDWRLKPQYQDSLNGLVQILMD
                     NPNIVIELGSHTDSRGTHEYNDSLSYKRAKAVVDYLISKGIEPDRLIPKGFGKRVPRV
                     LEEDKTVIIDGKEYTFPKGTVLTEEYINSLPSKDQREAAHQLNRRTTFQIIRDDYVPT
                     GEQNKPVQIEIIKEKTQ"
     gene            5572..6738
                     /locus_tag="PKI65_05865"
     CDS             5572..6738
                     /locus_tag="PKI65_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOB78002.1"
                     /translation="MMKSENAYDQYGASSQKQDVFEAIKILSRGISDTTFCNLFRSPF
                     DKQLISLHADGAGTKSALAYVYYKETGDIKVWENLAVDAIVMNTDDMLCVGITGPFYL
                     TNIIGRNKNLINLEVISAIIRGFQNFSEKMAEYDINLILTGGETADIGDLTKTLTIDV
                     VAASFISENDYIDNKNIKAGDVIIGFESTGQALWEDHPNSGIGSNGLTLARHTLLKNI
                     YKNKYPESYDYNTEEKLIYNGSFLLTDTSPIDSLDMGKYLLSPTKTYLPIVKEVFKYY
                     KKDIHGLIHCTGGGQLKVKRFVNNLRIVKNNLFNVPQLFRLIQTSTNLDWKQMYEIFN
                     MGHRLEMYISPELAKEIIIISEKYNVKAQIIGYVETADNTEIVIESDKNIYVYS"
     gene            complement(6785..7615)
                     /locus_tag="PKI65_05870"
     CDS             complement(6785..7615)
                     /locus_tag="PKI65_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOB78003.1"
                     /translation="MENNEKLIDVKKVIADKNPKLLKVFPNFIINYIKRIVHEDEINE
                     HIINNKGIDGIEFVKNTLFKLFGVNIQYEGLENLKKSQRYIVTSNHPMGGLDGLAIMY
                     VVSIVNPNIKVPVNDLLLYIPNLKTLFLPINKHGRQNVEAAKEIEKAYASDAVIIYFP
                     AGLASRKINGEIMDLEWKKSFIQKARQYQRDIIPVYTSGRNSNFFYNLANLRKKLGIK
                     SNIEMFFLSNEMFKQKDKNIKIIIGEPIPYTTFDKFKSDSEWAQYVKNIVYELRKKSE
                     "
     gene            7712..8374
                     /locus_tag="PKI65_05875"
     CDS             7712..8374
                     /locus_tag="PKI65_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOB78004.1"
                     /translation="MFAKDFIYTSFITVKTSTTLKEAKELMNEWKVSHLPIVNNDILL
                     GILNEDDLIGISDENTQLGNVELSTPIVSVQSDSHIFDILQFMGQYKLSICPVTAHNM
                     QYIGSIIGVDFLHCLSPFMSLNNPGGTIILEIKPVDYTMAQIASIIESNDAHILATFI
                     HADAEKNQLYVFIKIDKMDIAPIIQTFNRYDYIVYASYQEEEFYEELYRNYEFLMRFL
                     NI"
     gene            8381..9259
                     /locus_tag="PKI65_05880"
     CDS             8381..9259
                     /locus_tag="PKI65_05880"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOB78005.1"
                     /translation="MNVLIFFSQWRDIFMDPIKKICDFCGKENGIVGLYFFDHSEKKL
                     VKNKIHDIKNIIIEKTNLINEYCCIISLGGDGTLLDTLPLSLQYNLPVYGINFGKLGF
                     LTWGKPEHIEDYLIKIKNKEYKISKRGTIEINIKEHTLLFHNALNEITIKQYKEQNLI
                     SIDLFINDAFVCSYWADGLIIATPTGSTAYSLSCGGPIIEPTSENFVITPIAPHNLNS
                     RPLVIRDDTELKLEVKYPDIPILLTADANYHIIKMGQEIILKKSKKYFPLIEFEDNSF
                     YKVLREKLMWGQDSRR"
     gene            complement(9400..9777)
                     /locus_tag="PKI65_05885"
     CDS             complement(9400..9777)
                     /locus_tag="PKI65_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOB78006.1"
                     /translation="MKKTISTDLAPKAIGPYSQAIECNGLLFVSGQIPIDPNTGVIIT
                     GDIKMQTRRVLQNIKAILEAAGYTLDDVVKCTVLMADMNDFADMNQVYGEFFVNNKPA
                     RAAFGVVKLPMNAKVEIECIAYR"
     gene            9917..11302
                     /locus_tag="PKI65_05890"
     CDS             9917..11302
                     /locus_tag="PKI65_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOB78007.1"
                     /translation="MISQIIDYFSTWQDYHWLLALYTLITIIIALVGSGKRTTEDFLI
                     AGRKVGTLGFTTSVVASYIGGAALVAFSAYVFRFGLSALFVFLGTAIGFIIFIPYAKK
                     LKSWSEENITYTMMDWFYIKYDKKTGTLTTIVLMTVYLGMLLNQFIAGSAVLAGVSGW
                     TYERSLIFAALVLAVYLFIGGFQSVIRTDIFQYFVMMVIMIVLLRIIWMHDAPTVIPD
                     MYKNTQLDWGMMVAFLLFGILIVFQSAEYWQRVYAAKSIKVVKKGFWWSAILTFISGI
                     IITWIALSAKIANPNIAPTNAFGAGLVALLPQKLSALVLVMVFAAIMSSADTQIFVIS
                     SGFALNLFKKTNDDDKRLKKNTQIITLLVILLAVLIAYFIRDIVAVVNFITGIGFTVL
                     PAAWAAFHFDISPNIAFQSIIAGLIYVMLLIIFGIFIPEFVILSFIVSGLYMFFGNLI
                     VNKHVIKNNSL"
     gene            11299..12609
                     /locus_tag="PKI65_05895"
     CDS             11299..12609
                     /locus_tag="PKI65_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOB78008.1"
                     /translation="MKHKFLLIIFCFVVATNIYSQDTIVKGKRWNFLPVILYNSDLGF
                     EIGLLTSIYDFGDGRLYPNYIYNISYELDITTRGSIISQLFFDSGKKIKNGKWRIVSD
                     FSYIHEQASDFYGFNGYASNFHPEYMNDNNEAYISRMFYRYDRKIFRLINDVWYKLSN
                     QSSLIFGLHIFNYKIGTVNIDAINKFHQKKPLPDTTLLIDYYLNNNFIRDDEAEGGWI
                     IAPRVSYVYDGRDKIFNPTNGIYLEINLLANPEISSLKSKEFLRLSTNFKFFYSLFNK
                     HCIIALRLGYQDQIGAQAPFYFMPYITQAFSNNTLVEGLGGSKSLRGIYKNRAWGDGF
                     FYGNLEARILAISFKTKKQLGEIRVKPFYDFGIITDPVKIPINHINNDFIDYKDIPDR
                     LHHSLGLGVHLVLNDNFIISVDVGQSLYKNDGSTGVYVTTGYNF"
     gene            complement(12606..13139)
                     /locus_tag="PKI65_05900"
     CDS             complement(12606..13139)
                     /locus_tag="PKI65_05900"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOB78009.1"
                     /translation="MNTFLFYPYRIHHNNEWFRFITAGFIHGGILHLFINMWVLYIFG
                     IAIEKSLAMLLTNDKLHYIILYFGGMFFSSLIPYFKNKNNPQYMALGASGAISSIVFA
                     FILLYPTEKLIIFPIPIGIPAWIFGVFYLIFSYYQSRKSKDNIAHDVHFYGSLFGILY
                     LIIVKPSLLMHFINSIF"
     gene            complement(13447..13749)
                     /locus_tag="PKI65_05905"
     CDS             complement(13447..13749)
                     /locus_tag="PKI65_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOB78010.1"
                     /translation="MINKEKVIEILKTIYDPEIPINIWDLGIVYDIEITENNDIIITM
                     TLTAPNCPIADIILDDIKFSLKNLDEVNEVKINLTFDPPWSPNMMSEEAKLQLGLF"
     gene            complement(13739..14176)
                     /locus_tag="PKI65_05910"
     CDS             complement(13739..14176)
                     /locus_tag="PKI65_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOB78011.1"
                     /translation="MNSIEEKAKKIEEEFSLFDDWMDKYSILIDKAKNLKPLNPKYKT
                     PEYLISGCQSQLWLACEYIDGKLYCFADGDAIITKGMAAIVLELVNGETPENILNYDF
                     DVFENIGLNAHLSPTRSNGLYSLIKQVKLYAMDYLTNNKENDK"
     gene            complement(14184..15407)
                     /locus_tag="PKI65_05915"
     CDS             complement(14184..15407)
                     /locus_tag="PKI65_05915"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOB78012.1"
                     /translation="MHWNVKDFRQHFPFFNNNEQIVYLDNAATTQKNIETINKLNEFY
                     EYYNANVHRGVHYLSAKATELFEKSRIILQQYINAKYDHEIIFTKGTTEAINLVAHSY
                     IEKFCKAGDNIITTPIEHHSNFVPWQLAAERKELELRLIPFDIDEYRLNLDVYRNLID
                     KNTKIVAITQVSNVLGIETPLKEIIEIAHSKNVPVLVDAAQAFPHLQIDVQILDVDFL
                     ALSAHKFFGPTGMGILYGKEKWLDAMPPYQSGGEMIDNVTIENTTFNELPFKFEAGTP
                     NFADAIAWGATIDFINQLDRGQMFAYEEKLWNYAYQKLTKLPFVKIFALPDQKHYGAI
                     SFIIENIHSYDVASFLDELKIAVRVGHHCAQPLMNLLNVNSTIRASIAPYNTIEEIDR
                     LVEGLNIVYKFFIRN"
BASE COUNT         5522 a         2104 c         2271 g         5673 t
ORIGIN      
        1 tcttgcctgt gaggtattta cccatcacta cataccacat aatgtagttt tgcagatatt
       61 tggtagctac acccctaaat ttgaaatata tccaattagt gaattcacta gcctttttct
      121 tgacggtact taggctatat atacctcgct tttgcctatt gctagttact atttctttgt
      181 gcttgattac atttcttcta acctttttga atgctttttt attactgcta cacataacac
      241 catcttttgg catcttagcc ttcagaaatt gctctatctg ttctggactt aactttttgc
      301 tttcaaattt tttaaacaac aagttgccag ttctgtctgt agcagctaat acggctactt
      361 tctttggctt ttgcttccta gctcgcttca gttcttctgc atttttgtat attctgcctt
      421 tttcggaata atccatcaat agttcgtcca tctctactac tccgaaaaaa ttgatattat
      481 cttcgaaact ttgtagagct gctaatatct tgtgtcgcca agcgaagctt gttggtaagc
      541 tgatgcccac ttctttagca caagctctga gacttttgcc tgctaccata catctgatgt
      601 agtcgagcat tttttctttc ttttgtaacc tgtagacaaa agtaccggtg gtaactctaa
      661 actgtctgcc acagtctttg cagcgatagt tttgcatccc attttgtaaa ccatttttaa
      721 tgatgtgatt gcttcgacag tctgggcata gtggtggggc actgagtatt ttcagacctt
      781 gataatcgac tacatcagct tctaattttt tcattagaga ataaaggtta tctaagaatt
      841 ctttattctc ttccttactc atagcaaggt atttactggt attctctacg atttttttca
      901 tagcctaaaa tttttgcaaa aatataaaaa ttatttaaca tataaacttg aaaaacttaa
      961 acatttattg atttttttat tattggacag ttaaatatca accacaaagt tctcaaagaa
     1021 tgcacaatga acgcagagaa agatggaatt aaaaaggcta taaatagcac tttgtggtta
     1081 aaaaataacc taaataatta aaaaatagtt attttttgta acaatctaaa attttattac
     1141 ccacattatc cgttatagaa ccgtttacta ttttaattta tgtttgataa taaagcatta
     1201 gctcatatcc aaccaaatat taattgtatt atgaccgcta ttgtaaatgc aataataatt
     1261 tttatacctt ttatcaacaa aaatgttttc catgattctg ctaataatgg taagccagca
     1321 tgaccatctt gggtaatgtt attagctaat agtactgata taggcactat acctcctata
     1381 tacatgctaa taaatataaa atgaggtccc gaagctggta ttaatcctat taatatagat
     1441 attaatatcg ttaagtatat attatcatgt atccaatatt ctacgttaac atttttattt
     1501 attaatttga cagctattag tcctaataga atccaaaaaa ataatctaag aaaatgtttt
     1561 tttattacat gtccccacat atgctcttct atgaaatggt caggtaattt tataatagga
     1621 tatatcatta agagagctac tataactagt aaataattca aaataatttc tagcgaaaat
     1681 gcaccttcat gatgtccatg ttctttgttg ggtagtaaaa taataattat aaataatatt
     1741 aataaaaata gtaataatgc acgagatagg ctgagatgtt taatgttttc ttttatagaa
     1801 ctatatttca gattttcata atcatgctga tgtattacaa aatgctgatt atcagtagat
     1861 aaagtatgat ttttagtaat tttatcaatt aaaattcctg aaataaatga aataacaaat
     1921 aaaaataaaa atattaatat tgcctttata ggcattaagc caagcatagc aaagctttca
     1981 tcacctgtag ttgctataaa atttgctagc aatgcaccta ttgttatttc cccatgagta
     2041 aataaagaaa ctacaaaaaa actacccata cacccaggca atataccaag caaactacca
     2101 gttaatacac ttttccaact tttacttttt attcctttaa aaaattttcc cttggtcttt
     2161 atgtttaaat attctatcaa tagcataatt actataacaa aggatgttat ctgtatagca
     2221 ttaaaaacta caggcaacca ttcatttact aaagttctca ttttgatgat ttatgataat
     2281 ttcgaaatta ttgtcatccc aaaaaatttt aaattttaaa ttatcaagat tatgattact
     2341 tatattatca tatagcaatg tgacatttgc ttttttaaag atataatgta ttaattctga
     2401 acaataaaag gaactagaat caacaatatc aaaattatga tcaaaaggta tttgtctgtc
     2461 taaaaaataa tttgaccaat aaataagatt taggatatct aatgaatcta aatttttgta
     2521 cctagttatt attatagaat taggttgact atttcttata aaatgctcca aacaatcatc
     2581 ttgtacacca tttatcggtg ataaagatga tgatacactg tgtattactt tataattata
     2641 caaacaacta tcgcaaggaa ctataatgcc acaatgactt attttatatt cttcatttaa
     2701 taattttgct atgctttctg aaataacacc gtatcctaat ctcaaaatta tatcagcaga
     2761 ttgtattttg tctacctgag gagaagtaat gttatatgta gatgtgattg attttttatt
     2821 agcttttacc tctaaagctg atagatatat tataattatg ataataatag ttaataaata
     2881 tattataatt ttcttcatta atttaatatt attataaaaa caatttgcaa aaatataaaa
     2941 aagataaata agtttttaaa aaaaatcgca aaatttttta aataacaatt ttctatactt
     3001 ttataatatt agaattagtt cttatgttta attaatttaa tttatgaagt attttatatt
     3061 ttgattgtaa atcaattaaa ttttttattt atatttgcaa taaataagaa atcaaatatt
     3121 attaatacaa ttttaatttt atcttaattt aaattattta ttaaaaatgt tttaatttag
     3181 caaaaaaatt atatatgcaa agcaaaagta ttttcattac atttatttta atatttatta
     3241 ctagtttttt tgtttatgga caaaaaaatt tagttgaaga ggcagatgaa aaatttgaat
     3301 taaatcaatt ttatagtgct tcgcagttat atctaaaagc ctatcctaaa gtagataata
     3361 aattagaaaa aaatcgaatt ttataccagg taggtttatg ttattataat atgggtgatg
     3421 ctcgtaaagc attatcatat ttcaatcgtg tgataagggc tcaatatcct gatcctgaag
     3481 ttttttggta tacaggatta gtaaatatga ctttggcaaa ttatgaagaa gcgttgaaca
     3541 atttcgaaaa atacaaggga ttagtaccca acgatactct ggtggatatt aagatagaat
     3601 cctgtaagct atcaaaagcc tggatggata atcctacttt gtatgaggta acaagtgagc
     3661 gaaagctaaa cacaagaaat caagattttg ctacagcttg ggcagataaa aggtacagat
     3721 ctattatttt tacttctaat agggatggtg tagtaggtaa aggtatagat gaatggacag
     3781 gattaccttt tacagatttt tttattgcag aaaaagataa aaaaggcaat tggtcatcac
     3841 cagtaacggc agatgatcaa gaaatattaa atactcaaca taatgagggc gtgggatgct
     3901 ttaataataa atttaatact ttttatttca ctagatgtag agtcgaaaaa aagaaaattt
     3961 taggatgcaa tatttattat actactcgtc gtggtcgctc atgggcagag cctatattgt
     4021 taccatttac cactgattca actgctacag taggtcatcc ggcaatttct gatgatgaga
     4081 tgatgataat tttttctggt aatttatcag gaggttatgg aggtagagat ttatggatgg
     4141 ctacaagaac taaaaaaaat caggactttg gagaaccatc taatttagga cctataataa
     4201 atacaagtgg gaatgaaatg tttccaacct taagatatgt gaataataga acctatctat
     4261 atttttcatc agatggacat ccaggaatgg ggggattaga tttatatcgt tcagaatata
     4321 ttgatgggca gtggactgta cctgaaaatt tgcgttatcc cttaaattca cctcaagatg
     4381 attttcatat tatattttta gatgatccta caattttacg tacagaacaa gctcgtgaaa
     4441 tgggattttt atctagtaat agaaatttat cttccatgga cgatatttat tcattcaaat
     4501 tacccctcat atatttttct attagtggca tagtatatga tgatagtacc aaaaaaccaa
     4561 taccaggcgc tatagttaga attgaaggct cagatggtaa tatttacata gatactactg
     4621 ataacaatgg ttattatatt tttgataaaa ctcaaatatt cgaaaataat acttatacaa
     4681 tttatgtttc aaaagaagaa tatctaggcg aaaggggtat agaaactact gttggtttag
     4741 atagaagtaa agatcttgtg catgattttt atttgattcc tatacctaaa gagccaattg
     4801 tattgcctga tattttgtat gatttcgatg attggagact aaaaccgcaa tatcaggata
     4861 gcttaaatgg attagtgcag attttaatgg ataatcctaa tattgtaata gagctcggat
     4921 cacatacaga ctcgcgtggt acacacgaat ataatgatag cttgtcatat aaacgtgcta
     4981 aagctgtggt tgattatttg atatccaaag gaatagaacc tgatagattg atacctaaag
     5041 gttttggtaa aagagtccct cgagtactcg aagaggacaa aactgttata atagatggta
     5101 aggagtatac ttttccaaaa ggtacagttt taacagagga atacataaat tctctaccta
     5161 gtaaagatca acgtgaagcc gctcatcaac taaatagacg tactacattc caaatcatta
     5221 gagatgatta tgtgcctaca ggtgaacaaa ataaaccagt gcaaatagaa attattaaag
     5281 aaaaaactca ataatatctc ttgttttgga ttttaaatgt aggggttcaa atattttttg
     5341 ggcccctatt atttatataa aaaatcaata aataaagtaa aaattataaa ttctttactg
     5401 agataattta tttaaatatt ttatcttctc catttctttt tgataccttt ttttgtcttt
     5461 atttaaatat ttgtctctac tttttatata actttctatt ttaattattt tgtgttacta
     5521 aaaatactta atattctttt gttatttgta tttttgtgaa atttaatttt tatgatgaaa
     5581 tcagaaaatg cttatgatca atatggtgca tctagtcaaa agcaagatgt cttcgaagca
     5641 attaaaattt tatctcgcgg tatttctgat actacttttt gtaatctttt tcgctcacca
     5701 ttcgataaac aattaatatc attacatgct gatggtgcag gtactaaatc tgcattagct
     5761 tacgtatatt ataaagaaac tggagatata aaagtgtggg aaaacttggc tgtagatgct
     5821 atagtaatga atactgatga tatgctctgt gtcggtatta caggaccatt ttatctgaca
     5881 aatattattg gaagaaacaa aaatttaata aatctagaag taatatccgc aataattagg
     5941 ggttttcaaa atttttctga aaaaatggcg gaatatgata taaatcttat tttgactggt
     6001 ggtgaaactg ctgatatagg agatctcact aaaacactta caatagatgt ggtagctgca
     6061 tcttttatct ctgaaaatga ttatatagat aataaaaata taaaagctgg tgatgtgatt
     6121 ataggcttcg aatctactgg acaagctcta tgggaagatc atcctaatag tggtatcgga
     6181 agtaatggac taaccttagc tcgtcataca ttattaaaaa atatttataa aaataaatat
     6241 cctgaatctt atgattataa tactgaagaa aaactcatct ataatgggtc attccttttg
     6301 acagatacat caccaataga tagcttggat atgggaaaat atcttttgag tcctactaaa
     6361 acatatttgc ctattgtgaa agaagtcttc aaatattata aaaaagatat tcatggactc
     6421 attcattgca ctggtggtgg tcaacttaaa gtaaaacgat ttgtaaataa tttaagaata
     6481 gtaaaaaata atcttttcaa tgtaccacaa ttatttagat tgatacaaac ctctactaat
     6541 ctagattgga agcaaatgta tgagatcttt aatatggggc atagattaga aatgtacatc
     6601 tcgccagaat tagctaaaga gattattatt ataagtgaaa aatataatgt aaaagctcaa
     6661 ataattggtt atgtagagac tgccgataat acagaaatag tcatagaatc tgataaaaat
     6721 atttatgtct atagctaaac attttatgtt ttttatataa tataatgctc aagttttttt
     6781 atatttactc agattttttc cttaactcat atacaatatt ttttacatat tgagcccatt
     6841 cactatcaga tttaaattta tcaaaagtag tgtacggtat tggttcgcct ataatgattt
     6901 tgatgttttt atctttttgt ttaaacattt cgttagacaa aaaaaacatt tcaatattag
     6961 attttattcc taattttttt ctaagattag ctagattata gaaaaaattt gaatttctac
     7021 cagaagtata aactggtatg atatcgcgtt gatactggcg tgctttttga ataaaagatt
     7081 ttttccattc cagatccatt atttctccat taatttttct acttgccaag ccagcgggaa
     7141 aataaataat gacagcatcg ctagcataag ctttttctat ttctttggca gcttctacat
     7201 tttgtctgcc atgtttattt ataggtaaaa atagtgtttt caaattagga atataaagca
     7261 gtaaatcatt tactggaact ttgatattag gatttacaat gctcactaca tacattatag
     7321 ctaacccatc taagcctccc atagggtgat tagatgtgac tatatatctt tgtgattttt
     7381 ttaaattttc taatccttca tattgaatat ttacaccaaa aagtttaaat aaagtatttt
     7441 ttacaaattc gatgccatct atgcctttgt tattaataat gtgttcattt atttcatctt
     7501 catgcactat acgtttgata taatttataa taaaattggg aaaaaccttt aataacttag
     7561 ggtttttatc agctatgact tttttaacat ctataagctt ttcattattt tccataatat
     7621 ttcatattac tttgtaaaat cactatagaa atgtttagca aatataaata ttttttttgt
     7681 aattttgttt aatatgctaa aagatataaa aatgtttgca aaagatttta tttatacttc
     7741 ttttattaca gttaaaacat ctactacctt aaaagaagct aaagaattga tgaatgaatg
     7801 gaaagtatca cacttaccaa tagtaaataa tgatatattg cttggtattt taaacgaaga
     7861 tgatttaata ggtatcagtg atgaaaatac acaattaggt aatgtagagt tatctacacc
     7921 tatcgtatct gtgcaatcag attcacacat atttgatatt ctgcaattta tggggcaata
     7981 taaattatcc atttgtccag taacagcaca taatatgcag tatataggat ctataatagg
     8041 agtggatttt ttgcattgtc tatcgccatt tatgagttta aataatccag gaggcactat
     8101 aatactcgaa atcaagcctg tcgactatac tatggcgcaa atagctagta ttatagaatc
     8161 taatgatgct cacattttag ctacatttat ccatgcagat gccgaaaaaa atcaattata
     8221 tgtttttata aaaattgata aaatggatat agctcctatt attcaaactt ttaatagata
     8281 tgattatatt gtatatgctt cttatcagga agaagaattt tatgaagaat tatatcgcaa
     8341 ctacgaattt ttaatgagat ttttaaatat ttaactaaaa atgaatgtat tgatattttt
     8401 ttctcaatgg cgagatattt ttatggatcc aatcaaaaaa atctgtgatt tttgcggaaa
     8461 agaaaatgga atagttggtt tatatttttt cgatcatagc gagaaaaaat tagtaaaaaa
     8521 taaaatacat gatataaaaa atataattat agaaaagacc aatttgataa atgaatattg
     8581 ctgtataata tctttagggg gtgatggtac attattagat acattgcctt tgagtttaca
     8641 atataaccta ccagtgtatg gaatcaattt tgggaaatta ggatttttaa cttggggaaa
     8701 accagagcat attgaagatt atttaattaa aatcaaaaac aaagaatata aaatatcaaa
     8761 acggggaacc attgaaataa atatcaaaga acatacatta ttatttcata atgcacttaa
     8821 tgaaatcact ataaaacaat ataaagaaca gaatttaatt tcaatagatt tatttattaa
     8881 tgatgcattt gtatgttcat attgggcaga tggattaata atagctactc cgactggctc
     8941 tacagcatat tcattgagtt gtggaggtcc tattatagaa cctacttcgg aaaattttgt
     9001 aattactcct attgcacctc acaatctaaa tagtcgccca ctcgtaatac gagacgatac
     9061 agaattaaaa ttagaagtta aatatccaga tatacctatt ttattgactg cagatgctaa
     9121 ttatcacatt ataaaaatgg gacaagaaat catattaaaa aaatctaaaa aatatttccc
     9181 attaattgag tttgaagata attcatttta taaagtatta agagaaaaac taatgtgggg
     9241 acaagatagc agaagataat tatatttatt tggaaaataa taactttctt aaatcaactt
     9301 tttttttatc tactgcgctt ctttattttt tgtcattttt tatttattat ttgcataata
     9361 agaattgatt ttgctattaa gtggtagata aactcacatt tatctataag cgatgcattc
     9421 tatttctacc tttgcattca ttggtaattt cactactccg aaagctgctc tagcaggttt
     9481 attattaaca aaaaactctc catatacttg attcatatca gcgaaatcat tcatatctgc
     9541 cattagtact gtacatttca ctacatcatc tagggtatat ccagcagctt ctaaaatagc
     9601 ttttatgttt tgcaataccc tacgagtctg cattttgata tcacctgtta ttattacacc
     9661 tgtattcgga tctataggta tttgtccaga aacaaacaac aacccattac attctattgc
     9721 ttgactatat ggacctatag ccttaggtgc taggtctgta gaaattgttt ttttcataat
     9781 tcaatttttt ataaaagcaa atttataaaa aaatattaaa ttattaaaat gcttaatttt
     9841 atttttttta aagaaagatg taatttatca ttttgatatt acataatgaa aaaaaatcat
     9901 tttttttgta aaaaaaatga ttagccaaat tatagattac tttagtactt ggcaagatta
     9961 tcactggcta ttagcattat atacattaat tactataatc attgcacttg taggttcagg
    10021 caagagaaca acagaagatt ttttaatagc aggacgcaaa gtaggaacac ttggatttac
    10081 gacgtctgta gtagcttcat atataggagg tgctgcgtta gtagcctttt ctgcatatgt
    10141 atttagattt ggattatctg ctttatttgt atttcttggg acggcaatag gatttattat
    10201 ttttatacca tatgctaaaa aattaaaatc atggagtgaa gaaaatatta cttatacaat
    10261 gatggattgg ttttatatta aatatgataa aaaaactggt acacttacta ctattgtact
    10321 aatgacagta tatttaggaa tgttattaaa tcagtttata gctggatctg cagttttagc
    10381 tggtgtgagt ggttggactt atgaaagatc attaatattt gccgctctag tgcttgcggt
    10441 ttatttgttt attggtggtt ttcagagtgt gatacgaacc gatatatttc aatattttgt
    10501 aatgatggta ataatgatag tactactcag aattatctgg atgcatgatg ctcctacggt
    10561 aatacctgat atgtataaaa atactcaatt ggattggggg atgatggtag catttttact
    10621 ttttgggatt ttgatagttt ttcaaagtgc agaatattgg cagagagttt acgctgccaa
    10681 aagtatcaaa gttgttaaaa aaggtttttg gtggtctgca attttaactt ttattagtgg
    10741 tattataatt acttggatag cattatccgc taaaatagca aatccaaata tagcacctac
    10801 aaatgcattc ggagctggac tagtagcatt attaccgcaa aaattatctg ctcttgttct
    10861 ggttatggtt tttgcagcta ttatgagttc tgcagatacg cagatttttg ttatttcatc
    10921 aggatttgct ctaaatttat ttaaaaaaac caatgacgat gataaaagat taaaaaagaa
    10981 tacacagatt attacattat tagttatttt attagctgtt ttaattgcat attttattag
    11041 agatatagta gcagttgtta attttattac aggtatagga tttacagtgt tgccagcagc
    11101 atgggcagca tttcattttg atatttcacc taatattgcg tttcaaagta taattgctgg
    11161 attaatatat gtaatgttgt taattatttt tggcattttt atcccagagt ttgttatatt
    11221 atcatttatt gtgtctgggc tttatatgtt ttttggcaat cttattgtca ataaacatgt
    11281 gataaaaaat aatagtttat gaaacataaa tttttgttaa ttattttttg ttttgttgta
    11341 gcaacaaata tctattctca agatacaata gtgaaaggca aaagatggaa ttttttacca
    11401 gttattttat ataattctga tttaggtttt gaaattggtt tacttacgag tatttatgat
    11461 tttggagatg gcagattgta tcctaattac atttataata ttagttatga attagatata
    11521 actactcgag gtagcattat cagtcaacta ttttttgata gtggcaaaaa gattaaaaat
    11581 ggtaaatggc gtatagttag cgatttttca tatatacatg agcaagcttc agatttttat
    11641 ggatttaatg gctatgcatc taattttcat ccagaatata tgaatgacaa taatgaagct
    11701 tatattagca gaatgtttta tagatatgat aggaaaatct ttagacttat taatgatgtt
    11761 tggtataaat tgagtaatca atcatcttta atattcggat tacacatatt taattacaag
    11821 attggcacag taaatataga tgctattaat aaatttcatc aaaaaaaacc tctacctgat
    11881 acgacactat tgattgatta ttatttaaat aataatttta tcagagatga tgaggctgaa
    11941 ggaggttgga taatagctcc acgtgtatca tatgtttatg atggtagaga caagatattc
    12001 aatccgacaa acggtattta tttggaaata aatttattag caaatcctga aatttcatca
    12061 ttgaaatcca aagaattttt gagactatct acaaatttta aatttttcta ttcattattt
    12121 aataaacatt gtatcatagc tctcagattg ggatatcaag atcaaatagg agctcaggca
    12181 ccattctact tcatgccata tatcactcaa gcattttcaa ataatacatt agtagaagga
    12241 ctaggtggta gtaaatctct aagaggaatc tacaaaaata gagcctgggg tgatggattt
    12301 ttctatggta atttagaagc tagaatatta gccattagct ttaaaacaaa aaaacaatta
    12361 ggagaaatac gtgtgaagcc attttatgat ttcggtatta tcactgatcc tgtgaaaatt
    12421 ccaataaatc atataaacaa tgattttata gattacaaag atatacctga tagactacat
    12481 catagcttgg gtttaggtgt acaccttgtt ttaaatgata attttattat tagtgtagat
    12541 gtagggcaaa gcttatacaa aaatgatggt agcactggag tttatgttac aactggttat
    12601 aatttttaaa aaatactatt tataaaatgc attaaaagtg aaggtttcac tattatcaga
    12661 tataaaatac caaataaact accataaaag tgcacatcgt gagctatatt atctttgctt
    12721 ttacgagatt gataataaga aaaaatcaaa taaaagactc caaaaatcca tgcaggtatc
    12781 ccaataggaa taggaaaaat aatcaatttc tctgtgggat ataataatat aaaagcaaat
    12841 acaatagaag aaatggcacc tgatgctcct aatgccatgt attgagggtt gtttttattt
    12901 ttaaaatatg gaattaatga agaaaaaaac atgccaccaa aatataaaat aatatagtgt
    12961 aatttatcgt ttgttaataa catagctagt gatttttcaa tagcaatacc aaaaatatat
    13021 aacacccaca tatttataaa taaatgcaat atacccccat gaataaaacc agctgttatg
    13081 aaacgaaacc attcgttatt atgatgtatt ctgtatggat aaaatagaaa tgtattcatt
    13141 aggtttctat taaaaaaagc taaaatagag attaatatag tgattaataa aattttggta
    13201 gttatcataa aataaaattt ttaataatga gattgcaaaa ttataaattt taactcaaaa
    13261 cgattaatta ggtttcattt aaaccttata atctgtaatt gaaaagggag aattctaaat
    13321 tttaaataag ttgaactatg catattgtaa atatctacaa atttaaatat gttaaaaaaa
    13381 agctcatttt tgtttaatta tcgtgtcact gatttgtgtc tttagagttt tattaataaa
    13441 aaaactctaa aaaagtccta attgtaattt ggcctcttca gacatcatgt ttggtgacca
    13501 gggtgggtca aaggttaggt taattttgac ctcgttaact tcatcaagat tttttagaga
    13561 aaatttaata tcatctaata taatatcagc tattggacag ttaggtgctg tcaatgtcat
    13621 agtaatgatg atatcattat tttctgttat ttcgatatca tacactatac cgaggtccca
    13681 aatatttata ggaatttcag ggtcataaat agtttttaaa atttcgatta ctttttcttt
    13741 atttatcatt ttctttatta tttgtgagat aatccattgc atataatttc acttgtttta
    13801 ttaagctata caaaccatta gatctagtgg gagagaggtg agcatttaaa ccaatatttt
    13861 cgaaaacatc aaaatcataa ttgaggatat tttcaggggt ttcgccattc actaattcta
    13921 aaacaatcgc agccatccct ttagtgataa tagcatcacc gtcagcaaaa caatataatt
    13981 taccatctat atattcacaa gctagccaaa gttgagattg acagccacta attaaatatt
    14041 caggtgtttt gtatttagga tttagaggtt tcaaattttt agctttgtct atcaaaatac
    14101 tatatttgtc catccaatca tcgaatagag aaaattcttc ttctattttt tttgcctttt
    14161 cttcaatact attcatataa aaatcaattt ctaataaaaa atttgtagac aatattcaaa
    14221 ccttccacta atctatctat ctcttctatg gtattgtaag gagcaatgga tgctctaatc
    14281 gtgctattga catttaggag attcatcaaa ggttgagcac aatgatgccc tactctaacg
    14341 gcaattttta attcatctaa aaaactagca acatcataag aatgaatatt ttcaattata
    14401 aaagatatag ccccataatg cttttgatct ggtaaagcaa aaattttcac aaatggtaat
    14461 ttagttaatt tttgataagc ataattccac aatttttctt cataagcaaa catttgacct
    14521 ctatctaatt gattaataaa atcaatagta gctccccatg caatagcatc agcaaaatta
    14581 ggtgtgccag cttcgaattt aaaaggaagt tcattaaaag tagtattttc tatagttaca
    14641 ttgtctatca tttcaccacc gctctgataa ggaggcatag catctagcca tttttcttta
    14701 ccatacaaaa tgcccattcc ggtaggacca aagaatttat gagctgataa tgccaaaaaa
    14761 tctacatcta aaatttgaac atcaatctgc aaatgtggaa aagcctgagc tgcatctact
    14821 aaaacaggta cattttttga atgagctatc tctattattt cttttagagg tgtttcgata
    14881 cctagcacat tgctcacttg tgtaatagca acgattttgg tatttttgtc tattaaattt
    14941 cgatatacat ctagatttag cctatattca tctatatcaa aaggtattaa tcttaattct
    15001 aattcttttc tttcagctgc taattgccat ggtacaaaat tgctatgatg ttctatcggt
    15061 gtagttataa tattatcacc agctttgcaa aatttttcta tatagctgtg tgcaactaga
    15121 ttaatagcct cagtagtgcc tttagtaaaa ataatctcat gatcatattt ggcatttata
    15181 tattgttgta atataattct agatttctca aataattcgg tggctttagc agaaagataa
    15241 tgaacacctc tatgcacgtt tgcattataa tattcataaa actcgttcaa tttattaata
    15301 gtttcaatat ttttttgagt agttgcagca ttatctaaat aaactatttg ttcgttattg
    15361 ttaaaaaagg gaaaatgctg acgaaaatct ttaacattcc aatgcataaa aaaacaatta
    15421 ttatttttta aaaaacttta tatgcaaaag taaaacattt ttgttaacaa aggaaaattg
    15481 caattgttaa atttaatatt ggattaatat agaaataatt gttaatttta ccaaaattta
    15541 taattatgaa aaacatatta aaagatcaag
//
LOCUS       DAOPRN010000044        15517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_3212_length_15517_cov_124.129285, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000044 DAOPRN010000000
VERSION     DAOPRN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15517
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3212_length_15517_cov_124.129285"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..480)
                     /locus_tag="PKI65_05920"
     CDS             complement(37..480)
                     /locus_tag="PKI65_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78013.1"
                     /translation="MKTIEPLYFNKIIHLAKLDDYLSRFFEQNNLPDEIYPNIYLAIL
                     ETSKFLIMNITNEEDFKPIKLEVFFDNGILFSNISTNLLSELRMSFQLNKSDDESIML
                     IKNLCSSCQIKDNDTLQLTFAIDKLINEFEQIKDDEMQIYKKLKK"
     gene            complement(467..3766)
                     /locus_tag="PKI65_05925"
     CDS             complement(467..3766)
                     /locus_tag="PKI65_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78014.1"
                     /translation="MEGACNNRIINIIEKFAKKYYFNKLIKGLFWFILFAVILLIALA
                     SIENILWLKSTPRLILLIILITSLTIFFVHYVGIPLMRYFGLFRDKTYKDFALLLGYY
                     SDNDDKILTAVELFELNKKDDNELLINELQRRSNELSKINIKNFFKINEYYKWIISNL
                     GVVIVFIALSFTFPNQFIKPLVRITNYTKQFPKDYGFEINILNKDFLVESGKSFVLCF
                     EIEGEKIPKNIFIETLGNRYIPIKTPNNKYEYIFENLTENTNFKIIAEDWISDEIEIK
                     VYNRPSVYKLIANLYYPQYTNLKNEQLVNIFSINVPRGTKINFSIFIKDADSLIIINK
                     SIKNTYPIKSKFDYSIQILEDKNLKLLSKSIRSPIADTTNIYIAALPDAYPQIMVQVL
                     KDTFSIMHYYVQGSISDDYGFTKMLAIYRLQRNDSTIKGKIPIGISKNDLIQNFNFDI
                     DFSFLDLKPSDIVYFYLEVYDNDAISGPKATKSREFEVKIPTKEELQIEQSKQIQVAN
                     TKFAEMQKKLNNIRNELQQLENQWKLDRKFNYEQKQKWENLIKQQNEIIDELRNLEQN
                     FQFPSYYNENLEIYEKIKQIDELIKSINLDELNEYLKELEKLMQKNELKSEDIEEFKL
                     KNEELNKMLDRQIELLKRLDIEKNLRETFDELREFSKKFDELAKKIDPDEIKQQEELK
                     KELEKQFKKYDSILYENEKLNEPFKLDDIDIEKNNIYNEIQQADDNLKRNNDKKANEN
                     QKNTSKQLNDLAEKLENNLNQQLQEEYVEDAIILRQLLKNVLIASFEQENLIKNIFKQ
                     NPRLSNYQNYVRKQYELQEVMKRTKDSLYMLGKRQPLVSNFIFDEINKIEKESKKAIN
                     NLNNWILGQAAINQQNLMTSLNNLALFLADVLQQLDEQNSMSQMNSDGTMKCSSPKST
                     GNSTNTKPSMKTLRQLQQDLNKQMESLQKEMQNGKRQGKELSEALAKMAAQQEAIRRA
                     MQELKEQMTKEGNLKNSAFIQQMIEDMEKTEKELYNKNLTYETIKRQKQIEVRMLESE
                     KAEQQQEIDDIRKSETAKNDYKSNLIKNFQYNVIKNNNIESMNRGNLNFNFYYRKKIQ
                     EYFNNISYENN"
     gene            complement(3736..4302)
                     /locus_tag="PKI65_05930"
     CDS             complement(3736..4302)
                     /locus_tag="PKI65_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78015.1"
                     /translation="MKKKQIFILVIIVLFVILIIVAFLISKNSFNKSNGVYLKSISKN
                     NKLQIKNLLADSISVETISDKYSSGIKIENFFDNLKTELFIDSILLEGKDSIKISPIN
                     TSQYLYRLAIISMDSIKKGLVYENIFICELDSLYLTLNKINDSILINGFVYFNINDTN
                     YKSNYLLNFLNIKSKIIQRWKGHAIIEL"
     gene            complement(4307..5101)
                     /locus_tag="PKI65_05935"
     CDS             complement(4307..5101)
                     /locus_tag="PKI65_05935"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOB78016.1"
                     /translation="MKNNKANNKKQYKTRKYPKDYKKIKSDDSTTEIRLNKFIANCGY
                     CSRRDADELILTGSVLVNGKIVTQLGTKIKDTDKVMIGDAILKPEKKIYILMNKPKDY
                     ITTSKDPHNRKTVMSLLGDLQYRVYPIGRLDRNTTGILLFTNDGDLARKLSHPSSKII
                     KLYEVKLDKNLKFNDLQNIANGPIINGHKIYIDDISYIDNKPKSYVGIALHSGQNHIV
                     KRIFENFGYKVQSLDRTMYGPITKKSVPRGTWRYLTESEVRLLKRL"
     gene            5361..6884
                     /locus_tag="PKI65_05940"
     CDS             5361..6884
                     /locus_tag="PKI65_05940"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOB78017.1"
                     /translation="MTQNGELFKKLVSHCKEYGFVFPSSEIYDGLSAVYDYGQYGVEL
                     KENIKRYWWKSMVQFHENIVGIDTAILMHSKVWQASGHVDAFNDPLIDNKDSKVRYKA
                     DQLIEDYIDKLYEKAEKEIEKAKKRFGDNFDEQLFRTTNEKVKKYLDEAAKISNRLTI
                     LLDNNDLAGLKALIEELKIVCPVSGTCNWTEVRQFNLMFKTQLGSTADDAMTIYLRPE
                     TAQGIYVNFLNILKTSRQKVPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQYFVRP
                     GTEREWFEYWKEQRMQWHLSLGIPKEKYRWYEHKKLAHYANAAVDIEFEFPFGFKEIE
                     GIHSRTDYDLKRHQEFSGKKMLYFDHDLQESYIPYVVETSAGLDRTLLAVLTNAYTEE
                     ILPDNSLRTVLKIPNFLAPIKVAILPLVKKDGMNEYALNIFNDIKKYYLCQYDEKDSI
                     GRRYRRQDAIGTPYCITIDGQTMQDNTVTIRDRDSMLQDRVQVDNIVKYLNDKLNGL"
     gene            6881..7597
                     /locus_tag="PKI65_05945"
     CDS             6881..7597
                     /locus_tag="PKI65_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOB78018.1"
                     /translation="MNKKNKIIEKEHDQLNDNVLVEIINANIHLQDRIVLQGINFTLK
                     KGDFIYLIGKTGSGKSSLLKMLYADYPFNGEKGIVLGYDLANIKSKQIQALRRKIGIV
                     FQDFQLLIDRNIEKNLEFVLRATGWKNKNEINNRIDEVLEMVGLRDVRKKMPHQISGG
                     EQQKLVIARALLNNPELILADEPTGNLDLASSKDILEILINLKNNNRAIIMATHDLLS
                     IQSFPQKTYKCEDGTFTQMS"
     gene            7655..11149
                     /gene="dnaE"
                     /locus_tag="PKI65_05950"
     CDS             7655..11149
                     /gene="dnaE"
                     /locus_tag="PKI65_05950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOB78019.1"
                     /translation="MKNFVHLHLHTQYSILDGAANIERIFNKAQQLEMPAVAITDHGN
                     MYGVLDFYRTSKAYNVKPILGCEIYVARRSMANKENDIDRSGFHLILLAKNLTGYHNI
                     IKLVSKASLDGFYYTPRIDKELLTQYHEGLIASSACLGGEIPYYLRQNNIEKARQAVG
                     FYKDLFGEDFYLELQNHGLEEQAKINHEIKSLADEFGIKTIITNDVHFVNKEDKEAHS
                     LLIKLNTNRDDSTLLYTGNEYLKSYDEMLQCFPDDEASLLNTLEIAEKVESYSIENEV
                     ILPKYTLPDDFTDDFDYLKHLTYLGAKQRYGELLEAEVKGRIEYELEVIKKMDFAGYF
                     LIVQDYINAAREMGVLVGPGRGSAPGSIVSYCIGITNVEPLKYKLLFERFLNPERISM
                     PDIDVDFDDAGRQKVLNYVVEKYGNNRVAQIVSFQTMQARMAVRDVCRVKGIPLSKAD
                     KIAKLIPARPGTKLVNALEESPELKKLYDEDPEVKQVISIAMTLEGNVRSTGVHACGV
                     IISPSDITDFVPLAQAKESPIPVVQYEGSIIESAGMLKMDFLGLKTLSIIKDTLDLIY
                     RRRGKNIDIDKIPLDDNKTFELFQNGATIGVFQFESTGMQKYLRELKPTTIDDLIAMN
                     ALYRPGPMSYIPTYINRKHGKEPIEYYHDIMKEVLEDTYGIMIFQEQIMQLSEKMAGF
                     SKAEADTLRKAMGKKKPDLIEQMEVKFIDGCLKNNINRDIAKKVYEDMAQFGGYGFNR
                     SHTVAYSIIAYQTAYLKANFTDEFMASVLTHTSLNELNKILSECERLGIKVLGPDINE
                     SNTNFTVANNGIIRFGLSSIKYVGSIGAESIIAEREANGSFKNITDFAKRIDSKAINK
                     RVIEALAQAGAFDSLNLKRSQFFHVYDNNETTVDRLLRFANDYQASSKFTASLFDDDE
                     IITNFPMPDCEEWDYKTLIEKEHEALDFYLSGHPLDPISHLLNFFVTNTLSEINKTVD
                     DNKQIFFGGIVQSLQVKNDKNGNPFLIAEVADYNSNYSLRFFRNDFNNAQSLLKIDNP
                     YFFLCKWQINKNSNQAFLSVISILTVEEVQKAFDKAKIIISLLDPTDDDWAKFIELFD
                     TFDGNNMLIFNINTKGKSLFLNVDKKISFNFLKELENIKSVSSIKIYNKFLKDYIETL
                     INIEAELNEAEIYDLVDE"
     gene            11303..13126
                     /locus_tag="PKI65_05955"
     CDS             11303..13126
                     /locus_tag="PKI65_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOB78020.1"
                     /translation="MKLLKKLNFLISGYWKSVILYFFYNILTAFFSLFSIAMIIPFMR
                     ILFGMDTTIGYPGPFQLNAASIQLHANYFFAQLINNYGPVQTLGIVVLFVFLMILLKN
                     IFSYASIHVITTLRFGVVEKLRNMIYDKILRLPLSYFKTKEQGDIMTYIISDTQEIEV
                     SLISSLQFLFRDPITVIIFLASLFILNYKLTLIILLILPFIVIILGRIGKTLRKKSIR
                     SQRQLGLLMAIINETIDGLRVIKAFVAEDFMRKKMQKVNRSITKLNTKIFRRRSLSSP
                     LTETLSVMAILLILYIGSIIVLKDQSSFAPETFIAYILIFTQLIPPAKNISSGWYGVQ
                     KGLASLDRIEEILNYPETLTEDKNAIEKNTFDDEIKYNNVSFCYNPGHYVLKNINLTL
                     NKGTTTAIVGPSGSGKTTLVDLLPRFYDVTEGSITIDGVDIRKIKFDSLHRLFAIVNQ
                     DPILFNDSIYNNIAFGKPDATYEEVVQAAKIANAYDFIMETRWGFDTNIGEKGSKLSG
                     GQRQRISIARAVLTNAPILIFDEATSSLDSESEKLVQEAINNLIVNRTAIIIAHRLST
                     IINADQIVVLRDGEIVEIGNHTDLINNEDSFYRYLFEYQKF"
     gene            complement(13214..15241)
                     /locus_tag="PKI65_05960"
     CDS             complement(13214..15241)
                     /locus_tag="PKI65_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOB78021.1"
                     /translation="MFKKIILFLLFPFALFCQDPTSWTYELKKISNDEALIIFNVSID
                     EGWILYSLKTKGALPGEFYFEENPAYKLIGKTTESPNFKSKYEPDLGATTTFFEGAAT
                     FTQKIKILKPEKFDIEVFYSGQACLHDGMCVMIKKDFIIPIDGTKFIPLQSAEIKLKN
                     NNDTVIQDELSNKNIVEIENKSDTSIYTKIYGINPLDSTLKAHNEQYLIARSEAKAKS
                     QSLWGIFLAGILGGLIALLTPCVWPMIPLTVSFFIKRDKKKGIKDAIIYGLSIIVIYV
                     GLGLLVTLIFGANALNALSTNPWFNVFFFLLLLIFAIAFLGGFDLTLPSSWINAMDKR
                     AEKATGLLSIFFMAFTLVLVSFSCTGPIIGTLLVEAIVKGYLAPFIGMLGFSIALAIP
                     FTVFALFPKMISNLPKSGSWMNTVKVTLGFIELFFAFKFLSVADLASHWGILDREIFI
                     VIWVGIAFIMGLYFLDVVRFKGEEKKSHISVPRFFFALASFSFVFYLIPGLWGAPLKG
                     VSAFLPPMYTQDFNIDTKNIKADFKDLSPAIDFARNVNKPLLIDFSGYGCVNCREMEA
                     SVWTDPKVKDFLENNFVLVSLYVDDKTLLPPEESITINDGKIKTKLRTVGDKWSFLEF
                     TLAKKQSQPLYIIMDYNGKILTELYGYNKNPNDFLEWLQDGLEKFYQINKL"
BASE COUNT         5447 a         2040 c         2271 g         5759 t
ORIGIN      
        1 aaatttacaa agaaaatttt ataaaataca tatttttcat ttttttaatt ttttatatat
       61 ctgcatttca tcatctttta tttgttcaaa ctcattaatc aatttatcaa tagcaaaggt
      121 taattgtaaa gtatcattat cctttatttg gcatgaagaa catagatttt ttatcaacat
      181 tattgattcg tcatcagatt tattcagttg aaaactcatg cgcaattcag ataataaatt
      241 tgtagaaata ttagaaaata aaataccatt atcaaaaaag acctctagtt taatgggttt
      301 aaagtcttcc tcatttgtta tattcattat taaaaatttc gatgtttcta gaatagcgag
      361 atatatattc ggatatatct catctggcag attattttgt tcaaaaaatc tacttaaata
      421 atcatcgagt ttagcgagat gaattatctt gttaaaatat aatggctcaa ttgttttcat
      481 aagaaatatt attaaaatat tcttgaatct ttttacgata ataaaaatta aaattcaaat
      541 taccgcgatt catagattca atgttattat tttttattac attatactga aaatttttaa
      601 taaggttact tttataatca tttttagcag tttcactttt gcgaatatca tctatttctt
      661 gctgttgttc agctttttca gattctagca tacgtacttc tatttgtttt tgacgtttta
      721 tagtttcata agttaaattt ttgttatata attctttttc agttttttcc atatcttcga
      781 tcatttgctg aataaatgca ctatttttta aattaccttc tttggtcatt tgttctttta
      841 gttcttgcat agcgcgacgt atagcttctt gctgagctgc cattttagct agggcttcac
      901 tcaattcttt gccttgcctt ttaccatttt gcatctcttt ttgtaatgat tccatttgtt
      961 tatttagatc ctgttgtagc tgtcttagag ttttcatact aggcttggtg ttagtagagt
     1021 ttccagtgct tttaggacta ctacatttca tagtaccgtc actattcatt tgtgacatgc
     1081 tattctgctc atctaattgc tgtagtacat cagctaaaaa taaagctaag ttattcaaag
     1141 aagtcataag attttgttga ttaatggcgg cttgtcctag tatccaatta tttagattat
     1201 ttattgcttt tttactttct ttttctattt tattaatttc atcaaaaata aaattagaaa
     1261 ctaatggctg acgcttaccg agcatatata ggctgtcttt tgtacgtttc attacttctt
     1321 gcagttcgta ctgttttcta acgtaatttt ggtaattaga taatcgtgga ttttgtttaa
     1381 atatattttt gattagattc tcttgttcga aagaagcaat gagaacattt tttagcaatt
     1441 gtcttaatat tatagcatct tctacatatt cctcttgtaa ttgttgatta agattatttt
     1501 ccaatttttc tgctagatca ttaagttgtt tacttgtatt tttttgattt tcattagctt
     1561 ttttatcatt atttctttta aggttatcat ctgcttgttg tatttcatta tatatattat
     1621 ttttttctat atctatatcg tcgagtttaa atggttcatt tagtttttca ttttcgtata
     1681 atatgctatc atattttttg aattgttttt ctaattcttt tttaagttct tcttgttgtt
     1741 ttatttcatc aggatctatt ttttttgcaa gctcatcaaa ttttttacta aattctctta
     1801 attcatcaaa agtttctcta agattttttt caatatctag gcgttttagt agctctattt
     1861 gtctgtcaag cattttattt agttcttcat ttttgagttt aaactcttcg atatcctcag
     1921 attttaattc atttttttgc attaattttt ctaattcttt taaatattca tttaattcat
     1981 ctaaatttat agattttatt agctcgtcta tttgttttat tttttcatat atttctaaat
     2041 tttcattata gtatgatgga aattgaaagt tttgttctag atttctcaat tcatcgatta
     2101 tttcattttg ttgtttaatt agattttccc atttttgttt ttgttcgtaa ttaaatttac
     2161 gatcaagttt ccattgattt tctaattgtt gtagttcgtt tctaatatta tttaattttt
     2221 tttgcatttc agcgaatttt gtattagcta cttgtatttg ttttgattgt tctatttgaa
     2281 gttcttcttt agtaggtatt tttacttcaa attcacgtga tttagtagct ttaggtccgc
     2341 taatggcatc attatcatat acttctaaat aaaaataaac tatatcagaa ggtttgagat
     2401 ctaaaaaact aaaatcaata tcaaaattaa aattttgaat taaatcattt ttagagatac
     2461 cgattggtat tttgccttta atagtgctat cattacgttg caatctatat atagctagca
     2521 ttttagtaaa tccataatca tcagaaatag agccttgaac ataataatgc atgatagaaa
     2581 aagtatcttt tagtacttgt accattattt gcggatatgc atctggtaga gctgctatat
     2641 agatattagt agtatctgct ataggagatc gtatagattt gcttaataat tttaaatttt
     2701 tatcttctaa aatttgtata gaataatcaa atttactttt tataggataa gtatttttga
     2761 tagatttatt aattataatc aagctatctg catcttttat aaaaatagaa aaattaattt
     2821 tagttccacg tggaacatta attgaaaaaa tatttactaa ttgttcgttt tttaaatttg
     2881 tgtattgtgg ataatatagg ttggctatta atttataaac agaaggtcta ttataaactt
     2941 ttatttctat ctcatctgag atccaatcct ctgctatgat tttaaaatta gtgttttcag
     3001 ttaaattttc aaaaatatat tcatacttat tgtttggtgt tttgatagga atatacctat
     3061 tacctaaggt ttctataaaa atatttttag gaattttttc tccttcaatt tcgaagcaca
     3121 atacaaatga tttgccactt tctactaaaa aatctttatt taaaatgtta atttcgaaac
     3181 cataatcttt tgggaattgt ttagtataat tagttatcct gaccagtggt tttataaatt
     3241 gattaggaaa tgtaaacgat aaagcaataa agacaattac aacccctaaa ttacttatta
     3301 tccatttgta gtattcatta attttaaaaa agtttttaat attaatttta gatagctcat
     3361 tgcttcttct ttgtaattca tttattaata attcattatc gtctttttta ttcaattcga
     3421 ataattctac agcagtcaat attttatcat cattatcaga ataataacca agtaaaagtg
     3481 caaaatcttt ataagttttg tctctaaata atccaaaata tctcattaaa ggtataccga
     3541 cgtaatgaac aaaaaatata gtaagagatg taataagaat aataagaagg atcaatctag
     3601 gtgtagattt gagccataaa atattttcta ttgaagctaa agcgattaat aatatcaccg
     3661 caaacagtat aaaccaaaat aaacctttaa taagtttatt aaaataatat ttttttgcaa
     3721 atttttctat tatatttata attctattat tgcatgcccc ttccatcgct gaataatttt
     3781 tgattttata tttaaaaaat ttaataaata atttgattta taattagtat cattaatatt
     3841 aaaatataca aatccattta ttagtatcga atcattaatt ttatttagag ttaaatataa
     3901 agaatctaat tcacaaataa atatattttc atatacaagt ccctttttta tagaatccat
     3961 agatattatt gctaatctat aaaggtattg agatgtatta atgggagata tttttataga
     4021 atctttacct tctaataaaa tagaatctat aaatagttca gtttttagat tatcaaaaaa
     4081 attttcaatt ttaattcctg aactatattt atctgatata gtttctacgc ttatgctgtc
     4141 tgctaataaa tttttaattt gtaacttatt attttttgat attgatttaa gatatacacc
     4201 attagatttg ttaaatgaat tttttgaaat caaaaatgca actattatca atatgacaaa
     4261 aagaacaatt atgacaagaa taaatatttg tttttttttc atttaactac aatcttttta
     4321 ataatctaac ttcgctctca gtaagatatc tccatgttcc acgtggaaca ctttttttgg
     4381 taataggtcc atacattgtt ctatcgaggc tttgaacttt gtaaccaaaa ttttcaaaaa
     4441 ttcttttaac tatatgattt tggcccgaat gaagtgctat acccacgtat gattttggct
     4501 tattatctat gtaactgata tcatctatat aaatcttgtg accattaata ataggtccat
     4561 tagcaatatt ttgtaaatca ttgaatttta ggtttttatc taattttact tcatataatt
     4621 taatgatttt agaggatgga tgactgagtt tacgagctaa atcaccatca tttgtaaata
     4681 ataaaatacc agtagtattc ctatcgagtc taccaatagg atatacacga tattgcaaat
     4741 cgcctaatag agacattaca gtttttctat tatgtggatc ttttgacgtg gtgatatagt
     4801 cttttggctt attcatcaaa atatagattt tcttttctgg ttttaaaata gcatccccta
     4861 tcattacttt gtcagtatct tttattttag tacctaattg agtaacgatt ttaccattta
     4921 ctaagacact accagttaat ataagttcat ctgcgtccct tctagagcaa tatccacaat
     4981 tggctataaa tttatttaat cttatttctg tagtagagtc atcagatttt atttttttat
     5041 aatcttttgg atatttccta gttttatatt gttttttgtt atttgcttta ttgtttttca
     5101 taaatataat atttcatttt tacaaaatta ataaaaaaac aaggattttt aaagaaatca
     5161 ttatttatat aaatattttt acaaattata gaagcttcta aatcattttg cactaataga
     5221 tattgtgtat attaattgtt atttagaatt tgtttgtaat ttcgaaaatt aaaattaaaa
     5281 aatttccaca aaatcaggat gaaattaatt aaatttttta aaaatcaaaa aaactacgaa
     5341 atttgcataa aaaatattat atgactcaaa atggagaact atttaagaaa cttgtatcgc
     5401 attgcaaaga gtatggtttc gtatttccat catcagaaat atatgatggt ttatctgctg
     5461 tatatgacta tggccagtat ggagtagagc ttaaagaaaa tattaagcga tattggtgga
     5521 aaagcatggt gcagtttcat gagaatattg tagggataga tacagctata ctaatgcact
     5581 ctaaagtctg gcaagctagt ggacatgtag atgcattcaa tgatccttta atagataata
     5641 aagatagtaa agttagatac aaagcagatc aattgataga agattatatt gataagctct
     5701 atgaaaaagc tgaaaaagaa atagaaaaag ctaaaaaaag gtttggtgat aattttgatg
     5761 agcaattatt tagaactact aatgagaaag taaaaaaata tttagatgaa gctgccaaaa
     5821 ttagtaatag attgacaata ttattagaca ataatgattt agctggattg aaggccttaa
     5881 tagaagagtt aaagatagtc tgtcctgtta gtggtacttg taattggaca gaggttagac
     5941 aatttaatct aatgtttaaa actcagcttg gtagtactgc tgatgacgct atgacaattt
     6001 atcttcgacc agagacagct caaggtatct atgttaattt cttaaatata ttaaaaactt
     6061 cacgtcaaaa ggtgcctttt ggtattgcac aaataggtaa agcttttaga aatgaaatag
     6121 ttgctagaca atttatattt cgtatgcgtg aatttgaaca gatggagatg caatattttg
     6181 tgcgacctgg tacagagaga gagtggttcg agtattggaa agaacaaaga atgcaatggc
     6241 atctctctct gggaatacca aaagaaaaat atcgctggta tgaacataaa aaattagctc
     6301 attatgctaa tgcagctgta gatatagaat ttgaattccc ttttggattc aaagaaatag
     6361 aaggtattca ttcccgtacc gattatgatc ttaaacgcca tcaagagttt agtggtaaaa
     6421 aaatgttata ttttgatcat gatttacaag aatcgtatat cccttatgta gtagaaactt
     6481 cagcagggtt agatagaaca ttgctagctg tattgactaa tgcatatact gaggagattt
     6541 tgccagacaa tagtttgcgt acagtactca aaattcctaa ttttttagca cctattaaag
     6601 tcgctatact tccattagta aaaaaagatg gtatgaatga atatgctcta aacatcttta
     6661 atgatataaa aaaatattat ctttgtcaat atgatgagaa agatagtata ggacgccgtt
     6721 atcgtcgtca agatgctatt ggtaccccct attgtattac tattgatgga caaactatgc
     6781 aagataatac agttacgata cgtgatagag atagcatgct gcaagatcgt gtacaagtgg
     6841 ataatattgt taaatatctt aatgataaat tgaatggttt atgaataaaa aaaataaaat
     6901 tatagaaaaa gaacatgatc agttaaatga taatgtatta gtagagataa taaatgcaaa
     6961 tattcattta caggatcgta tagttttgca agggatcaac tttacattga aaaaaggtga
     7021 ttttatttat ttaattggta aaactggtag tggtaagtca tcattgctga aaatgcttta
     7081 tgcagattat ccttttaatg gagaaaaagg tatagtgcta ggatatgatc ttgctaatat
     7141 taaatctaaa caaattcaag ctttacgtag aaaaataggt attgtatttc aagattttca
     7201 attgttaata gatagaaata ttgaaaaaaa tttagaattt gtactcagag ctacaggatg
     7261 gaaaaacaaa aatgaaataa ataatagaat tgatgaagta ttagaaatgg taggcttgcg
     7321 agatgtgaga aaaaaaatgc ctcatcaaat ctcaggtgga gaacagcaaa aattagtaat
     7381 tgctagagct ctacttaaca atccagagtt gatcttagcc gatgaaccta ctggcaattt
     7441 agatttggct tcgtcaaaag atattttaga aattttaata aatttaaaaa acaataatag
     7501 agccattata atggctactc acgatttact ttcgatacaa tctttccctc aaaaaacata
     7561 taaatgtgaa gatggtactt ttactcaaat gagttaaaaa aaaaaacaat tttatttctc
     7621 attaattttt tatattttta caaaaaagta aaaaatgaaa aactttgtgc accttcattt
     7681 gcatacacaa tattctatac ttgatggagc agctaacata gaacgaatat ttaataaagc
     7741 acaacaatta gaaatgcctg cagtagctat tactgatcac ggcaatatgt atggcgtttt
     7801 agatttttat cgtacatcaa aagcatataa tgttaaacca atattaggtt gtgagattta
     7861 tgtggctaga cgttctatgg ctaataaaga aaatgatatt gatagatccg gttttcatct
     7921 aattttgctt gctaaaaatt taactggtta ccataatatc attaaattag tttctaaggc
     7981 atcactagat ggattctatt acactccaag gatagataaa gagttactca cccaatatca
     8041 tgaaggttta atagctagtt ctgcttgttt gggtggtgaa ataccttatt acttaagaca
     8101 aaataatatc gaaaaagcta gacaggcggt tggattttat aaagatttat ttggtgaaga
     8161 tttttatttg gaattgcaaa atcatggttt agaagaacag gctaaaatta atcatgaaat
     8221 aaaaagttta gcagatgaat ttggtattaa aactattata actaatgatg tacattttgt
     8281 aaacaaagaa gataaagagg cgcatagttt attaattaaa ttgaatacta atagagatga
     8341 tagtacttta ttatatactg gcaatgaata tctaaaatct tatgacgaaa tgctacaatg
     8401 ttttcccgat gatgaagctt cattattgaa tacattagaa atagctgaaa aggtagaatc
     8461 ttattctatt gagaatgagg taattttgcc taaatacaca ctaccggatg attttacaga
     8521 tgattttgat tatttgaagc atctcacata tttaggtgca aaacaaagat atggagaact
     8581 tttggaagca gaggtaaaag ggcgcataga atatgagctt gaagtgataa aaaaaatgga
     8641 ctttgctggt tattttctaa tagttcagga ttatataaat gctgctagag aaatgggtgt
     8701 attagtaggt ccaggacgag gctcagcacc aggatctatt gtttcatact gcataggtat
     8761 cactaatgta gaaccattaa aatataaatt attatttgag cgttttctaa atcctgaacg
     8821 tatttctatg ccagatatag atgtagattt cgatgatgct ggtagacaaa aagttttgaa
     8881 ttatgtagtt gaaaaatatg gcaataatcg tgtagcccaa atagtatctt ttcagactat
     8941 gcaagctcgt atggctgtac gcgatgtgtg tcgtgtaaaa gggatcccac tgtctaaagc
     9001 tgataaaatt gctaaactta taccggcgcg tcctggtact aaacttgtta atgctttaga
     9061 agaatcacca gaattaaaaa aattatatga cgaagatcca gaagtgaaac aagttattag
     9121 tattgcgatg acactggaag gcaatgtgcg ttctacgggt gttcatgctt gtggggttat
     9181 cattagtcca tcagatatta cagattttgt tccattggct caagcaaaag aatctcctat
     9241 acctgtggtg caatatgaag gttccattat agaatctgct ggtatgctaa aaatggactt
     9301 tttaggactc aaaactttat ctattattaa agacacatta gacctaattt atagacgtag
     9361 aggcaaaaat attgatattg acaaaatacc attagatgat aataagactt ttgaactatt
     9421 tcaaaatgga gcaacaatag gtgtatttca atttgaatct actggtatgc aaaaatattt
     9481 gcgagagttg aagcctacta ctattgatga tttaatagct atgaatgccc tttatcgtcc
     9541 tggacctatg tcttatatac caacttatat taatcgtaag catggtaaag agcctataga
     9601 gtattatcat gatatcatga aagaagtgct agaagatacg tatggcatca tgatttttca
     9661 ggagcaaata atgcagctga gtgaaaaaat ggctggtttc tctaaagccg aagctgatac
     9721 gctacgaaaa gctatgggca agaaaaaacc cgatctgata gaacaaatgg aagttaaatt
     9781 tattgatggg tgtttaaaaa ataatattaa tcgagatatt gcaaaaaaag tttatgaaga
     9841 tatggctcag tttggaggct atggttttaa tagatctcat acagtagctt attctatcat
     9901 tgcatatcaa actgcatacc taaaggctaa ttttacagat gaattcatgg caagtgtact
     9961 cacacatacg agtttaaatg aacttaataa aatcctaagt gagtgcgaaa gattaggcat
    10021 caaagtgcta ggtccagata ttaatgagtc aaatacaaat tttactgttg cgaataatgg
    10081 tatcattcgt tttgggttga gtagtatcaa gtatgttggt agtatcggtg ctgaatctat
    10141 aattgcagaa agggaggcta atggatcgtt taaaaatatt actgattttg ctaaacgaat
    10201 agattcaaaa gctataaata aacgcgtaat agaagcttta gcacaagctg gtgcctttga
    10261 tagtttaaat cttaaaagat ctcaattttt tcatgtctat gataataatg aaactaccgt
    10321 agatcgtttg ttacgtttcg caaatgatta tcaagcatct agtaaattca ctgctagttt
    10381 atttgatgat gatgagataa taactaattt tcctatgcca gactgtgaag aatgggatta
    10441 taaaacttta atagaaaaag aacacgaagc tcttgatttt tatctctcag gtcatccact
    10501 tgatcctatc agtcatttat taaacttttt tgttactaat actttgtctg aaattaataa
    10561 aaccgtcgat gataataaac aaatattttt tggaggtata gttcaatcgt tacaagtgaa
    10621 aaatgataaa aatggtaatc catttttaat agccgaggta gctgattata attctaatta
    10681 ttcacttcgt ttttttagaa atgattttaa caatgctcag tcattattga aaattgataa
    10741 cccttatttt tttctttgta aatggcagat taataaaaat tctaatcaag cttttctatc
    10801 agttatctct attttaactg ttgaagaggt gcaaaaagct tttgataaag ctaaaattat
    10861 tatttcactt ctagatccta ctgatgatga ttgggccaaa tttattgaat tatttgatac
    10921 ttttgacggt aataatatgc taatatttaa tataaatacg aaggggaaaa gtttgttttt
    10981 gaatgttgat aaaaaaatat cttttaattt tttaaaagaa ttagaaaata tcaaaagtgt
    11041 aagctctatt aaaatttaca ataaatttct aaaagattac atagagacat taattaatat
    11101 agaagcagaa ttaaatgaag cggagatcta tgatttagta gatgagtaat agtggacaac
    11161 caaataaaag atttctttta tctaatttat tattcgtagt atatagaatt ttagtattct
    11221 aaaatttttg catttttgtt tgctttatca ttttgattat aaataatatc attaatctta
    11281 ttatttttgc aaaaaataat ttgtgaaact attaaaaaaa ttaaattttt taatatcagg
    11341 atattggaaa tctgtcatat tgtatttctt ttataatata ttgactgcat ttttctcatt
    11401 attttctatt gccatgataa taccatttat gagaatacta tttggtatgg atactactat
    11461 aggatatcct ggtccttttc agttaaatgc tgcatctatt caattgcatg caaattattt
    11521 tttcgctcaa ttaataaata actacggtcc tgtccagact ttaggtatag tagttttatt
    11581 tgttttttta atgattttat taaaaaatat atttagttat gcttctattc acgttattac
    11641 taccctgagg tttggagttg tagagaaatt gagaaatatg atttatgata aaattttgcg
    11701 actaccacta agctatttta aaactaaaga gcaaggcgac attatgacat atattatcag
    11761 tgatacccaa gagatagagg tttctctgat ttcttcatta cagtttttat ttcgtgatcc
    11821 tatcactgta ataatatttt tagcatcatt atttatacta aattataaac taactcttat
    11881 aattctttta atattaccat ttattgttat catattaggg cgaattggga aaacattacg
    11941 aaaaaaatct attcgtagtc aaagacaatt gggtttattg atggcaataa ttaatgaaac
    12001 tattgatgga ttgcgtgtaa taaaagcatt tgtggcagaa gattttatgc gtaaaaaaat
    12061 gcaaaaggtt aatcgatcta tcaccaagtt aaatacaaaa atctttcgtc gtcgtagtct
    12121 cagtagccca ctcacagaaa cactctctgt gatggctata ttacttattt tatatattgg
    12181 ttctatcata gtattaaaag atcaatctag cttcgctcct gaaactttta tagcctatat
    12241 acttattttc actcaactca ttccaccagc taaaaatata tcttcaggtt ggtatggtgt
    12301 tcaaaaaggt cttgcttctt tagataggat agaagagata ctcaattatc cagaaactct
    12361 cacagaagat aaaaatgcta tagaaaaaaa tacttttgat gatgagataa aatataataa
    12421 tgtatcattt tgttataatc caggtcatta tgttttaaaa aatattaatc ttacgttaaa
    12481 taaaggtact actacagcta ttgtaggtcc tagtggttct ggtaaaacta ctttagtaga
    12541 tttattgccc agattttatg atgttacaga aggtagtatt acaatagatg gtgtggatat
    12601 tagaaaaata aaatttgatt cactgcatcg tttatttgct attgtaaatc aagatcctat
    12661 actttttaac gatagtatat ataataatat agcttttggc aagccagatg ctacttacga
    12721 agaagtggtg caagctgcta agattgctaa tgcttacgat tttattatgg aaacacgctg
    12781 gggctttgat actaatatcg gcgaaaaagg atctaaactt agtggtggac aacgacaacg
    12841 tattagcata gctcgagcag ttttgactaa tgcacctata cttatctttg atgaagctac
    12901 tagttcgctt gacagtgaaa gtgaaaaatt agttcaagag gctattaata atttgatagt
    12961 taatagaacc gctataatta tcgcacatag attatctact atcattaatg ctgatcaaat
    13021 agtagtgcta agagatggag aaatagtaga aatcggtaat catactgatt tgattaataa
    13081 tgaggatagt ttttatagat atctgtttga ataccaaaag ttttaatcaa atttaatgat
    13141 tttttataaa cagagaatat atatctgaat aagtttataa cccaaaaatt attttttaaa
    13201 ttgatttaag tttttataat ttattaattt gataaaactt ttctaatcca tcttgtagcc
    13261 actctaaaaa atcattagga tttttattat aaccatacaa ttcagtcaat attttaccat
    13321 tataatccat aattatatat aaaggttgag attgtttttt agctagagta aattctaaaa
    13381 aactccactt atcacctaca gttcttaatt ttgttttaat tttaccgtca ttaattgtaa
    13441 tgctctcttc tggaggcaat aaagttttat catctacata aagagaaact aggacaaaat
    13501 tattttctaa aaaatctttt accttaggat ctgtccatac actagcttcc atttctctac
    13561 aattaacgca gccataacca ctaaaatcta taagaagagg cttgttaaca ttgcgtgcaa
    13621 aatctatagc aggtgataaa tctttaaaat cagcttttat atttttggta tctatgttaa
    13681 aatcttgcgt atacataggt ggcaaaaaag ccgaaacacc tttaagtggt gctccccata
    13741 gaccaggaat cagataaaaa acaaagctaa aagatgcaag ggcaaagaaa aacctaggaa
    13801 cgctaatatg tgattttttc tcctcccctt tgaaacgtac tacatccaaa aaatataaac
    13861 ccattataaa agctattcca acccatataa cgataaatat ttctctatct aaaatgcccc
    13921 aatgactagc taaatctgct acagacaaaa acttaaacgc aaaaaataat tctataaaac
    13981 caagagtaac ttttacagta ttcatccagc taccagattt aggtaaattc gaaatcattt
    14041 taggaaataa agcaaagaca gtaaaaggta tagccagagc aattgaaaaa ccaagcatac
    14101 ctataaatgg tgctagatag cccttaacaa tagcttctac taaaagtgtg cctatgatgg
    14161 gtccagtaca actgaatgat acaagtacta aagtaaaagc cataaaaaag atactcaata
    14221 atccagtagc cttttctgct cttttatcca tagcattaat ccaagagcta ggaagtgtaa
    14281 gatcaaaacc acccaaaaat gcaattgcaa aaattaataa taataaaaag aagaatacat
    14341 tgaaccaggg attagtagat aaggcattta aagcattagc accaaaaatt aaagtaacta
    14401 ataatcccaa ccctacataa ataacaatta tagataatcc ataaataata gcatctttta
    14461 ttcctttctt tttatctcgt tttataaaaa aacttacagt tagaggtatc ataggccata
    14521 cacaaggtgt taataatgct attaatcccc ctaatattcc tgctaaaaaa attccccata
    14581 aagattgtga tttggcttta gcctctgaac gtgcaatcaa atattgttca ttatgtgctt
    14641 ttagagtaga atctaaagga ttaataccat atatttttgt ataaatagat gtatcagatt
    14701 tattttctat ttctactata tttttgtttg ataattcatc ttgtataact gtatcattat
    14761 tattttttaa tttaatttct gcagattgca atggtataaa tttagtacca tcaattggga
    14821 ttataaaatc tttttttatc attacacaca taccatcatg taagcaagct tggccagaat
    14881 aaaatacttc aatatcaaat ttttccggtt ttagtatttt tattttttgt gtaaatgtcg
    14941 ccgcaccttc aaaaaaagtt gttgtagctc ctaaatctgg ttcatatttt gatttaaaat
    15001 taggcgattc agtggtttta ccaattaatt tatatgcagg attttcttca aaataaaatt
    15061 cgcctggtaa ggcaccttta gtttttaaag aatataaaat ccaaccctca tctatcgata
    15121 cattaaaaat aattaaagct tcatcattag agattttttt taactcatat gtccaagaag
    15181 tgggatcttg acaaaataaa gcaaaaggga ataaaaggaa tagaattatc tttttaaaca
    15241 taatttttta aaatttgttt tgcaaaaata aaaaaaatca ttaaacaaaa taaaaattat
    15301 taataatatt agataaatgt atgattagtt cagaaaatct aagtcttatg atttattagt
    15361 taaatcaaat aattaaatat atttaatatc tatgaaatag tgttttggaa tattatactt
    15421 agatataatg gtttttgtat gtcgattaat gaaggtgaaa agttttatgt taaatttaat
    15481 attggcttag ctctcaagtc cctgtataaa aaccacg
//
LOCUS       DAOPRN010000045        15378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_3250_length_15378_cov_121.038439, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000045 DAOPRN010000000
VERSION     DAOPRN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15378
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3250_length_15378_cov_121.038439"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKI65_05965"
     CDS             <1..345
                     /locus_tag="PKI65_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78022.1"
                     /translation="MCSSNKKAFKKVGRNVIKHKEIVTNHRQKRGIYSLSTVKKKASE
                     FTNWIYFKFRGVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRE
                     LVNKTYVDFNIY"
     gene            complement(790..1734)
                     /locus_tag="PKI65_05970"
     CDS             complement(790..1734)
                     /locus_tag="PKI65_05970"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOB78023.1"
                     /translation="MNNNDGLKETNFSFPKQKKIYKGKVRDIYQINGLLLMIVTDRIS
                     AFDIVLPQPIPYKGQVLNQIAEYFLDTTSDILPNWKIICPHPMCMLGHKAKPFGIEMV
                     IRGYLSGHAWREYKYGKRVLCGQYLPDGLKENDPLPRPIITPTTKAIEGHDEDISPGE
                     IINLGLASKEEYEKLEQYTYALFKRGTEIANQKGLILVDTKYEFGKLGDEIILIDEIH
                     TPDSSRYFYIEGYEQRQKEGLPQKQLSKEFIREWLMEQGFTGQDDEKPPIMPTEVILE
                     VSKRYIKLYEILTGKTFQPINYNKLEEEIYKSVLKYLN"
     gene            complement(1778..2494)
                     /locus_tag="PKI65_05975"
     CDS             complement(1778..2494)
                     /locus_tag="PKI65_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOB78024.1"
                     /translation="MKKTIILFICMLFCLSIDIKAQKDESLTDFKFFRCGMYGTLGPS
                     WMKPDMKDWKNEGMRFGYGFGFMAEFAVAPNLVIASGFDVMYGSGKMKLSDTLFSYDN
                     MTDKLSNINSIYKIQYLQIPITLKMRTNEINYFRYYMRVGGSLGLRIGTKYTEEISAQ
                     DNSFIFRTYDNEKANNRISLFRATFDIGAGIEYSLGGTTALLGEIYFNNGLTNSLKEY
                     KNIKYKSILNYLMFKIGILF"
     gene            2547..3581
                     /locus_tag="PKI65_05980"
     CDS             2547..3581
                     /locus_tag="PKI65_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78025.1"
                     /translation="MKKLVMVVILFSMFFASCNSCSKTKEREFLPSIERKKLHKLYLP
                     PIKIQRYEQDLFAIPLDSLKNRLTALYPKYSFFYRLEDLENPANLFQIKLYLTDPVVK
                     QLKKDVDKQYADLDWLEKELYEAFKRILYFDSTFKIPKVFTYISGGDFEFPVKYAENN
                     LIIALDMYLGQSYPIYAQWGIPRYISYRMTKDFIIIDCMKEIARAYVEKYTPPTSTLL
                     DQMLYHGKILYFTDLTLPYIADSIKIYYTTPQYIWASKNEGNVWGFFIDKKLLYTTDM
                     REIMKYIGEAPFTSTFSQYSAPRMGQFIGWQIVRSYAKNHPKENFKDIFAITNSSILL
                     QQSHYKPKKL"
     gene            3731..4612
                     /locus_tag="PKI65_05985"
     CDS             3731..4612
                     /locus_tag="PKI65_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78026.1"
                     /translation="MRWIFTSLFVFVISCFSLLIKPFYYKNVLNNKNINIENYQKIKL
                     LELPMSETIIPLGFTSDAKKILGVKISIGSDLYSLEDISQVPFVVYQLNNGKSELIEG
                     NIDQLKKLDINGDFLISIKNKETETIDIYALELDNGELLRYECLSFNTNANDIFGAYS
                     PNRQKIYFSSDMLGGYGGYDIYEVEHLGAGKWSTPRNLGPNVNTSGHEIAPFIMSDGL
                     TLFFSRKPSLNNDNYDIYVSTIMDDGTIEVADIIDTNINTNGDDLFIRISPNNNKAVY
                     VTKMKNSWCFYEINFHQ"
     gene            complement(4629..5225)
                     /locus_tag="PKI65_05990"
     CDS             complement(4629..5225)
                     /locus_tag="PKI65_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOB78027.1"
                     /translation="MMDEYLTIRSQAKSIYKDRNSKFIGLLYPIENLDEVKNYLQIAK
                     KEYYDATHHCYAYILGVEKEIQFDSDAGEPSGSAGKPILNALLSADITNILAVVVRYF
                     GGTKLGISGLINAYKSATLLAIENSEIITKYIYDIFNVSIPYTQQQLFFNLQKKYEFE
                     FEIINADNIGQDIQIKIRKSKRDLLIKDCDNIGFIISE"
     gene            complement(5235..6098)
                     /locus_tag="PKI65_05995"
     CDS             complement(5235..6098)
                     /locus_tag="PKI65_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOB78028.1"
                     /translation="MLLFLLALITSLAILVSFKIFEKIEVSTPHAIVINYLVAFILGY
                     FYQPFNFQKLPSFDWFYLSVASGIILSISFFIYSLSIKYFGISITALSGKMSIIIPIL
                     IGFFLLGDPVLAHRIMGIIMIFPALYLLLFETKNDKNKITKLKYLIFPILLFLFTGCN
                     DSIFKIADTYFLLGDNANIYMYLTNAFAISLVVAIIFVLFQKPKFLKKQLFKTSLAGI
                     ELGILNWYSTYFFLKAIRFLPLTLFIPLFNISFVLLSVILGRILFKEVLNKRKIIGTF
                     IAILSIILLSL"
     gene            6248..6871
                     /locus_tag="PKI65_06000"
     CDS             6248..6871
                     /locus_tag="PKI65_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78029.1"
                     /translation="MTPEQLSLFIEIIGYIASAFVLISVMMRSIVKLRWYLLIGNIFY
                     VIYGVLINAMPVILLNAINGVLNIYFLYQAHKQFGDFEIIKISPHENIVMYFVNHFKE
                     DIKKFFPDFENLKTNEENYVLMRDNVIVGLFSFKQSYDQVNITLDYVTPAYRDLKPAK
                     FLFYKSEFFKTRGITQLITVSNVPYHKKYLNKIGFNNIDNNKFILKI"
     gene            6924..6998
                     /locus_tag="PKI65_06005"
     tRNA            6924..6998
                     /locus_tag="PKI65_06005"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6959..6961,aa:Val,seq:tac)
     gene            complement(7194..8129)
                     /locus_tag="PKI65_06010"
     CDS             complement(7194..8129)
                     /locus_tag="PKI65_06010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOB78030.1"
                     /translation="MKIECAYCLTSLNSESLFKVSDNLLYVCPECGSVTLAPNQHINI
                     YPVSYFGSDTKKFSFPLVQKIIDIFQLKQGKRLAKWIDKDANVLDIGCGDGQLLTAIS
                     KKRRGKFIGIELENKALHRAKLNSKISVIAQDIFYYEPNLQFDIIICNHSFEHIGKPI
                     QLMEQINKLLKTNGIFYINIPNTDSIQFHLFRSKWLHLDPPYHDNIPNIKKFIEVLLN
                     KGFIIKKNNHWNFIHNISSFILSSGNYIFKQFNYFVNILKNPYPIFKNILYILLMILL
                     TVLLIVPAILEFIISGLLKKGATVEIVAQKIEDLE"
     gene            complement(8135..8266)
                     /locus_tag="PKI65_06015"
     CDS             complement(8135..8266)
                     /locus_tag="PKI65_06015"
                     /inference="COORDINATES: protein motif:HMM:NF028380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HOB78031.1"
                     /translation="MGKGDKRTKRGKLFSGTYGKSRPSKRNKKRMQKNKTKQNEGQE"
     gene            complement(8297..8434)
                     /locus_tag="PKI65_06020"
     CDS             complement(8297..8434)
                     /locus_tag="PKI65_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78032.1"
                     /translation="MKKILFALLILLIVLLTSCKAKETCPAYHHGSSAQIEIVISDDI
                     G"
     gene            8850..10118
                     /locus_tag="PKI65_06025"
     CDS             8850..10118
                     /locus_tag="PKI65_06025"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOB78033.1"
                     /translation="MQIHKFGGGSLKNPQSIEQAIKIIKQRNNEPTLVVISAFAKTTN
                     ALENILYLARNDQDYQDELNKLKKFHLNITLQFFNDKNASIFDEIDQYFQQLEHALYN
                     YKDWEYDIHYDATVSFGEILSTIIIHNVFDLYGLANIWLDAREVIITDTLHRDAIINW
                     DLTNKMINEKVKPLLRDNNLIITQGFIGRSIDGYISTLGREGSDFSAAIFANCLNAEC
                     VTIWKDVPGIFNADPKIFDNAILLTHLNYKEAFELAYYGAKILHPKTIQPLEEKKIKL
                     LIKNFLDPDTKGTLIDDYTTKDKHISSCIIKKNQVLISISPKDSLFISENDLSLILFI
                     FSDFNIKINLMQTDAFHFHVCIDNNYFKITSLINRLNEKFNTEIKHNLFLVTFTHYKN
                     AIFNILHNENIYIDQYNGDIRQCLTDSIIL"
     gene            complement(10263..11372)
                     /gene="gcvT"
                     /locus_tag="PKI65_06030"
     CDS             complement(10263..11372)
                     /gene="gcvT"
                     /locus_tag="PKI65_06030"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOB78034.1"
                     /translation="MKYTPFTKIHESLGAKMVEFAGFYMPMQYKGIQEEHIAVRERVG
                     VFDVSHMGEIYIKGQNALDFAQYVTSNDVSALKDGKIQYSCFPNGKGGIVDDLLVYRI
                     NEQEYLLVVNAANIEKDWNWLCSHAPKFNIEIGKDLINESDNIAQLAIQGPLAMKIMQ
                     KIVEEPIMDMKHYTWQYVTIANIKDILLSTTGYTGSGGCEIYFDPKHATIIWEAIFEA
                     GKDEEIIPAGLGARDTLRLEMGFCLYGNDIDDTTSPLEAGLGWIVKFVDHKNFIDKEF
                     LLKQKQQGIKRKLIGFEMVDKGIPRHDYPICNDKGDIIGNVTSGTFAPYVKKAIGLGY
                     VPIDFSDPDTEIFINIRNKLTKAKVVTPPFYKPNK"
     gene            11617..12705
                     /gene="lpxK"
                     /locus_tag="PKI65_06035"
     CDS             11617..12705
                     /gene="lpxK"
                     /locus_tag="PKI65_06035"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOB78035.1"
                     /translation="MKYILYPFAFIFYLIQKIRKFLYSIKICKRYIPPITTINIGNLS
                     FGGNGKTPHTIYTARLLLNNNYKVAILLRGYKRKTRGFIEVNDDASVVDVGDEALVYK
                     RNFGSLVRVAVCENRAKGIRILLDKYPDLDIILLDDAMQHWRVNSHLQLLLTDFSNPF
                     FRDHLFPVGQLREPRRSSKRADIIIITKFPSIFSPIIFKNLTEKIKLYPHQQLFVSYI
                     SYNKLKLVFNNNKNIEIYDTKPYAIFVFTAIANPQSLNEHLLLMCKDLIRFNFPDHHQ
                     FTEKDVIKLKKAFNDYPSANKIIVTTEKDIPRLELPSIKKYLLDLPIYYAPIDIRFHN
                     MANNEYNYDKYILDYVRKNQKNNRIFKE"
     gene            12662..13474
                     /locus_tag="PKI65_06040"
     CDS             12662..13474
                     /locus_tag="PKI65_06040"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOB78036.1"
                     /translation="MLEKIKKTTEFLKSKVDLKPEIGIVLGSGLGNLGNEINPVAVIP
                     YKEIPNFPISTVMGHGSNLIFGTLEGKNVVAMQGRFHYYEGYTMQEITFPIRVMKALG
                     VQSLFLSNAAGGMNPDFEVGDIMLIVDHINFMGTNPLIGPNYDELGPRFPDMSEPYSK
                     KFINLAKQCANNLNIPIKEGVYVAVTGPNFETPAEYKFFRIIGGDAVGMSTVPEVIVA
                     AHSGLPVFGVSIITDLGGFEDIQPISHEEVLKAADAAEPKLSAIIKAMLKEL"
     gene            13542..15080
                     /locus_tag="PKI65_06045"
     CDS             13542..15080
                     /locus_tag="PKI65_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOB78037.1"
                     /translation="MLLKVFSAAIFGIDAIIVTVEVDLAPGTKTFISGLPDNAVKESR
                     NRIEPAIINSGFLFPGKKITINFAPADIRKEGSHYDLPVALGILGANNQLPDVDWSEY
                     MIVGELSLDGSILPVHGILPIAIKAKRNNFKNLIIPKANIREASVIEGLNIYGFDNLR
                     EVIDFFHGNFIPDNNKTAIKESISEHTCESIIDFADVKGQENVIRAMEIAAAGRHNIL
                     MIGPPGAGKTMIARRLPTIMPPMTFEESIETTQIYSVAGKIVNNGGLITERPFRAPHH
                     TISDVALVGGGAIPKPGEISLAHNGILFLDELPEFKRSVLEVLRQPLEDRVINISRAK
                     YATTFPANFMLVAAMNPCPCGFYNHPEKQCTCGHGAIKKYLNKISGPLLDRIDIHIEV
                     VPLVFKELSSSKTSHSSKEIRNNAMRAYEIQKDRYKNLPKIYSNAQLHSRLIKKFCPV
                     KDEGMVLLKNAMEKLSLSARAYDRILKLARTIADLDESEIIKPYHLAEAIQYRTLDKS
                     NWGL"
BASE COUNT         5607 a         2087 c         2103 g         5581 t
ORIGIN      
        1 atgtgtagca gtaataaaaa agcattcaaa aaggttggaa gaaatgtaat caagcacaag
       61 gaaatagtaa ctaaccatag acaaaagcga ggtatatata gcctaagtac tgtcaagaag
      121 aaagctagtg aattcacgaa ttggatatat ttcaaattta ggggtgtagc taccaaatac
      181 ctgcaaaact acattatgtg gtatgtagtg atgggtaaat acctcacagg caagactgta
      241 agcaatactg ggcgaatgct caatctcgca tcctcggaca ggtgggcgtg gtatcggtat
      301 agagagttag tgaataaaac atatgttgat tttaacatat attagattcc cgaatttcac
      361 cccgccttcc caaaatttta aaaacgcatc tcaaaatctt actatttaat aatttatgaa
      421 aatttatatt agaacagcta ataaataaat attgagaaat agataaaagt ttaaattatt
      481 tttattgaat tgagcgttta accatatata attattatta tatcctaacc aaaataatag
      541 aataacgttg catttgtttc aattagttta ggtattttat catctggtaa cttctataat
      601 tttaagttat ttttatgata ataaacttct caatttctcc acctacaaac tactctacac
      661 cttcacatat taactttccc attctctctt tgccacgatt atagtttatt atagaattca
      721 taataaaatt aatggttcgg tatatttatt gttattaact aaacgtctat ttcaaaaaat
      781 attaattagt taatttaaat attttaggac acttttatat atttcttctt ctaatttatt
      841 ataattaatt ggttggaaag ttttaccagt gagtatttca tatagtttaa tgtatctttt
      901 agatacttct aatatcacct cagtaggcat tattggtggt ttttcatcat cttgccctgt
      961 gaaaccttgt tccattaacc attctcggat aaattcctta gataattgtt tttgtggtaa
     1021 gccttctttt tgtctttgtt cataaccttc tatgtagaaa tatcttgacg agtctggagt
     1081 atgtatttcg tctattaata ttatttcatc gcccaatttg ccaaattcat atttggtatc
     1141 tactaaaatt aatccttttt gattggctat ttctgtacct cttttaaata gagcataagt
     1201 atattgttct aatttttcgt attcttcttt agatgcaagt cctaaattta taatttctcc
     1261 aggtgagata tcttcatcgt gtccctctat agctttggtg gtaggagtaa tgatagggcg
     1321 ggggagagga tcattttctt ttaaaccgtc aggtaaatat tgtccacaaa gcactctttt
     1381 accatattta tattcgcgcc atgcatgtcc gctaaggtat ccacggataa ccatctctat
     1441 cccaaagggt tttgctttat ggcctagcat acacatcggg tgtggacaaa taattttcca
     1501 gtttggtaaa atatcactcg tagtgtctaa aaaatattct gctatttgat ttagcacttg
     1561 tcctttgtat ggaattggtt gtggtaaaac gatatcaaat gctgaaatcc tatctgtaac
     1621 tatcattaag agtaaaccat ttatttgata aatatccctt actttacctt tataaatttt
     1681 tttttgtttt ggaaaactaa aatttgtctc tttcaaacca tcattattat tcatatattt
     1741 ataattttat tgtaatataa taaatttttt taataattta aaataaaata cctattttaa
     1801 acattaaata atttaatata gatttatact taatattttt gtattctttt aggctattag
     1861 tgagaccatt attaaaatat atttctccta ataaagcagt agtaccacct aatgaatatt
     1921 ctattccagc acctatatca aaggttgccc tgaaaagtga tatacgatta tttgcttttt
     1981 cattatcata agtgcgaaaa ataaacgaat tgtcttgagc tgatatttct tcggtatatt
     2041 tagttcctat acgcaatcct aatgagcctc ctactctcat ataatatcta aaataattga
     2101 tttcattagt tcgcattttc aatgttatgg gaatttgtaa atattgaatt ttgtaaatag
     2161 aatttatatt agataattta tctgtcatat tatcataact aaataaagta tcagaaagtt
     2221 tcatttttcc actgccatac attacatcaa agcccgaagc tatcactaaa tttggtgcta
     2281 cggcaaattc tgccataaag ccaaaaccat aaccaaatct catgccttca tttttccaat
     2341 ctttcatatc aggcttcatc cagctggggc ctagtgtacc atacatacca catctaaaaa
     2401 atttgaaatc tgtcaatgat tcatcttttt gtgctttaat atctatagat aaacaaaata
     2461 acatacagat aaacaaaatt attgtctttt tcatttcttt gattaatttt gaaaattata
     2521 aaacaaaatt ataaaattaa atttttatga aaaaattagt aatggtagtt attttgttct
     2581 ccatgttctt tgcttcgtgc aattcttgca gcaaaactaa agaaagagaa tttttacctt
     2641 cgatagagcg taaaaagtta cataaattat acttaccacc tatcaaaatt caaagatacg
     2701 aacaagatct ttttgccatt ccgcttgata gtttaaagaa tagacttaca gcattatatc
     2761 ctaaatatag ttttttttat agattagaag atttagaaaa tcctgctaat ttatttcaaa
     2821 taaaattata tttaacagat ccagtagtta agcaattaaa aaaagatgta gataaacaat
     2881 atgctgattt agattggtta gaaaaagaat tgtacgaagc ttttaagcgt attttatact
     2941 ttgatagtac attcaaaatt cccaaggttt tcacttatat atctggcgga gatttcgaat
     3001 ttccagtaaa atatgctgag aataacctca taatagctct agatatgtat ttgggtcaat
     3061 cttatcctat ctatgctcaa tggggtatac ctcgatatat atcatatagg atgactaaag
     3121 attttataat aatagattgt atgaaagaaa tagcacgtgc ttatgtagaa aaatatactc
     3181 cgcctactag tactctctta gaccagatgc tatatcacgg caagatattg tatttcacag
     3241 accttacatt gccatatata gctgatagta tcaaaatata ttatactaca ccacagtata
     3301 tttgggctag caaaaatgaa ggtaatgttt ggggcttttt catagataaa aaattactct
     3361 ataccaccga tatgagagaa atcatgaaat atatagggga ggcacctttt actagtactt
     3421 tttcacaata tagtgctcca cgcatggggc agtttatagg ctggcagatt gtgcgttctt
     3481 atgctaaaaa tcatcctaaa gagaatttta aagatatctt tgctattact aattcgagta
     3541 ttcttttgca gcagagtcat tataaaccta aaaaattata aaaaattagt tctttaacaa
     3601 taagaaaagt caaattaata cttagatgtt aatattatgt atataaaaaa aatcaactag
     3661 tctaaatatt aaaaaaaatc gtttctatta catagttttt atatattttt agcaaaaaat
     3721 ttctaaaaaa atgcgatgga tatttactag tttatttgtt tttgttattt cttgctttag
     3781 cttattaata aaaccatttt attataaaaa tgtattaaat aataaaaata ttaatattga
     3841 aaattatcaa aaaataaaat tattagaatt gcccatgtct gaaactataa ttcctttagg
     3901 atttacttct gatgctaaaa aaatattagg agttaaaatt tcaataggat ctgatttata
     3961 ttccttagag gatatatctc aagtaccatt tgtagtttat caattaaata atggaaaatc
     4021 tgaactaata gagggtaata ttgatcaatt aaaaaaacta gatattaatg gagatttttt
     4081 aatatctatt aaaaataaag aaacagaaac aatagatatt tatgctttag agttggacaa
     4141 cggagaatta ttaagatatg aatgtttgtc atttaatact aatgctaatg atatatttgg
     4201 tgcatatagt cctaatagac aaaaaatata tttctctagt gatatgctag gtggctatgg
     4261 aggttatgat atctatgaag tggaacatct aggagctggt aaatggtcta caccgagaaa
     4321 tctaggccca aatgttaata ctagtggtca tgaaattgca cctttcataa tgagtgatgg
     4381 acttacacta ttttttagtc gtaaaccttc attaaataat gataattatg atatttatgt
     4441 atctactatt atggatgatg gcacaataga agtagcagat atcatcgata ctaatataaa
     4501 tacaaatggt gatgatttat ttattcgcat tagcccaaac aataataaag ctgtatatgt
     4561 aacaaaaatg aaaaattcgt ggtgtttcta tgaaataaat tttcatcaat agatagtaag
     4621 tttatttttt attccgatat tataaaccct atattatcac aatcttttat cagaagatct
     4681 cgtttgcttt ttctaatctt aatttgtata tcttgtccta tattatctgc atttataatt
     4741 tcaaattcga attcatattt tttttgtaaa ttaaaaaata attgctgctg agtataaggt
     4801 atactgacat tgaaaatatc ataaatatat ttagttatga tttcactgtt ttcaatagct
     4861 aataatgtag ctgatttata agcatttatc agcccagaga ttcctagttt agtaccacca
     4921 aaatatctaa ctactacagc taaaatatta gtaatatctg cggataataa agcatttaga
     4981 attggcttgc cagctgagcc tgaaggttct cccgcatcac tatcaaactg tatttctttt
     5041 tctacaccta aaatatatgc atagcagtga tgggtagcat cataatattc tttttttgct
     5101 atttgtaaat aattttttac ttcatctaaa ttttctatag gatacaataa accaatgaat
     5161 ttgctatttc tatctttata tatagactta gcttgacttc tgatcgtcag atattcatcc
     5221 atcatgcaat aaatttataa actcaataat attattgata atattgcaat aaaagtacca
     5281 ataatttttc ttttatttaa tacttctttg aataaaatcc ttcctaatat tacactcaat
     5341 aaaacaaaac taatattgaa taatggaata aatagtgtta gaggtagaaa tctaatagct
     5401 ttcaaaaaga aatatgtaga gtaccaattt aaaattccta gttctatgcc cgcgagacta
     5461 gttttgaata attgtttttt taaaaattta ggtttctgaa ataatacaaa aataatagct
     5521 acgactaaag aaatagcaaa agcatttgtc agatacatat atatattagc attgtctcct
     5581 aataaaaaat aagtatcagc tatcttgaag atactgtcat tgcagccagt gaaaaggaat
     5641 aaaagtatag gaaaaatcaa gtattttagt ttggtaattt tatttttatc atttttagtt
     5701 tcgaataata aaagataaag cgcgggaaaa atcataataa tccccataat gcgatgtgct
     5761 aaaacaggat cgcctaatag aaaaaaacca ataagtatgg gaataataat agacattttg
     5821 ccacttagag ctgtgatgct gatgccaaaa tattttatag atagagaata aataaaaaaa
     5881 gaaattgaca aaattatacc actggctact gacaaataaa accaatcaaa tgaaggtaat
     5941 ttttgaaaat taaatggttg ataaaaatat cctaatataa aagcaactaa ataattaatg
     6001 acaatggcat gtggtgtaga cacctcgatt ttttcaaaaa ttttaaaact tactaggata
     6061 gctagactag tgatgagagc gagcagaaat aaaagcatat tttttagcaa aattaagtat
     6121 ttattaaatg tatgatcaaa tattttttta ttattattaa tttagaattt tttttacaat
     6181 tttgtaattt atttgtaaaa tttattttaa aaaatttgtt tttataattt taataaatat
     6241 aaaaaatatg actccagaac aattatcttt attcatagaa atcataggtt atatagcttc
     6301 ggcttttgtg ttgatatcag taatgatgcg ttctattgta aagctgcgat ggtatctact
     6361 gattggaaat attttttatg taatatatgg tgtattaata aatgctatgc ctgttatatt
     6421 attaaatgct attaatggtg tattaaacat ttattttttg tatcaagcgc ataagcaatt
     6481 tggcgatttc gaaatcatca aaatttcacc tcacgaaaat atagttatgt attttgttaa
     6541 tcatttcaaa gaagacataa aaaagttttt tcctgatttt gaaaatttaa aaacaaatga
     6601 agaaaattat gttttgatga gagataatgt tatagtggga cttttttcat ttaaacaaag
     6661 ctatgaccaa gtaaatatca ctctagatta tgtgactcct gcatacagag atctaaagcc
     6721 agctaaattt ttattttata aatccgaatt tttcaaaaca agaggtataa ctcaattaat
     6781 cacagtctct aatgtacctt atcacaaaaa atatttgaat aagattggtt ttaataatat
     6841 agataacaat aaatttattt taaaaatcta ataaaaaaat ttgtattatt aaaattttta
     6901 ttatctttgc aatctagatt ttagggcgct tagctcagct ggttcagagc gcctgcctta
     6961 caagcaggag gtcaatggtt cgaatccatt agcgcccatt tttttacttt atcgaaaatc
     7021 aagcttttta gaaaaaaaat tcaaaaatcc taaatattta tatatcatag tagattttat
     7081 taattattta ctaaaaataa atcttaatta aatttttaaa atcaatgaga tttgtgagta
     7141 taaaatattg ttaattataa atatatttat aattaacaat aaaagatata gtatcactct
     7201 aaatcttcaa ttttttgagc tactatctct acagtagctc ctttttttaa taaaccacta
     7261 ataataaatt ctaaaatagc tggcactata agtagtacag tcaataatat cattaataag
     7321 atgtataaaa tattcttgaa aataggataa ggatttttta aaatattgac aaaataatta
     7381 aattgtttga aaatataatt accagaactt aaaataaaac ttgaaatatt atgtataaaa
     7441 ttccaatgat tatttttttt aataataaaa cctttattta acaaaacttc aataaatttt
     7501 tttatattgg gtatattatc atggtatggt ggatctaaat gaagccattt ggatcgaaat
     7561 aaatgaaatt gtatactatc agtattaggt atgttaatat aaaaaatgcc atttgtcttt
     7621 aataatttat taatttgttc cattaattgt attggtttac caatatgctc aaagctatga
     7681 ttacatatta taatgtcaaa ttgtagatta ggttcatagt aaaaaatatc ttgagctatc
     7741 acagaaattt ttgaatttag tttagcacga tgtaatgctt tgttttccaa ttcaatacct
     7801 ataaacttac cgcgtctttt tttagatata gcagttaata actgtccatc tccacatcct
     7861 atatctaata cattagcatc tttatctatc catttggcaa gtcgtttacc ttgttttaat
     7921 tgaaaaatat ctattatttt ttgtaccaaa gggaatgaaa atttcttagt atcagagccg
     7981 aaatatgata ctggatatat atttatatgt tgattgggag ctaatgttac actaccacat
     8041 tcaggacata catataataa attatctgaa actttaaaaa gagactcact atttaacgat
     8101 gttaaacagt aagcacattc aattttcata aattttattc ttgaccttca ttttgttttg
     8161 tcttattttt ttgcattctt tttttattac gtttagaagg tctgctcttt ccgtatgtac
     8221 cactaaataa tttgcctctt ttagttcttt tatcaccttt tcccataatt atttttttaa
     8281 tttttttttt aaaatttcat cctatatcat ctgatattac tatttctatc tgtgctgatg
     8341 agccatgatg atatgctggg caagtctctt tggctttgca agaggttaat agcacaatta
     8401 ataatattaa taatgcaaat aaaatttttt tcattttttc atttttttgt aactgcaaaa
     8461 ttatatattt ttttttaatc taaaaaatta ttaaaaaagt atattactaa tgctctaaat
     8521 agtaatattg attatgtata aactatattt acttttattg agtttttttc taatttatgt
     8581 tgcatatact ttactctcta taaaattatt cagtttttca ttacatctaa atattcggca
     8641 aaaataatac tatcaatata ttatagataa tagaccattt catttatttt aaaaataggt
     8701 aaaagacgaa ttgtgatatt tatatatact taaagagatt attaaatccg attttcatat
     8761 atcgtaacaa acaaaacaaa tcgtattgat tatcaatgta taattttttt aattttacat
     8821 cgaaaaaaac tagtaaattt gtaataaaaa tgcaaattca taagtttggt ggtggaagct
     8881 taaaaaatcc acaatctatc gaacaagcta taaaaattat aaaacaaaga aataatgaac
     8941 cgacattagt agtaatctca gcatttgcta aaactactaa tgcattagaa aacattcttt
     9001 accttgccag aaatgatcaa gattaccaag atgaattaaa taaattaaaa aaatttcatt
     9061 tgaatattac tttacaattt tttaatgaca aaaatgcttc gatttttgat gaaatagatc
     9121 aatattttca gcaattagaa catgctttat ataattataa agattgggaa tatgacatac
     9181 attacgatgc tactgtatct tttggcgaga tattatctac aataataatt cataatgttt
     9241 ttgatttata tgggttggct aatatatggt tagatgctcg agaagtaatc ataacagata
     9301 cattgcatag agatgctatc ataaactggg atttaactaa taaaatgata aatgaaaaag
     9361 taaaaccatt actaagagat aataatttga taataactca aggttttatt ggtcgttcaa
     9421 tagatggtta tatatccaca ttaggacgag aaggcagcga tttttcggca gcgatttttg
     9481 ctaattgcct taatgctgaa tgtgtaacta tctggaaaga tgtaccggga attttcaatg
     9541 cagatcctaa aatttttgat aatgcaatac ttttgacaca tttaaattat aaagaagctt
     9601 tcgaattagc ttattatgga gctaaaatat tacatcccaa aactattcag ccattagaag
     9661 aaaaaaaaat aaaattatta ataaaaaatt ttttagatcc tgatactaaa ggtacattga
     9721 tagacgatta tactactaag gataaacata tctcttcttg tattattaaa aaaaatcaag
     9781 ttcttatttc tatttcgcct aaagattcac tttttattag tgaaaatgat ttatccttga
     9841 ttttatttat tttttcagat ttcaatataa aaattaattt aatgcaaacc gatgcttttc
     9901 attttcatgt atgtatcgat aataattatt ttaaaattac gtcacttata aatagattga
     9961 atgaaaaatt taatactgaa ataaaacata atttattttt agttactttt acccattata
    10021 aaaatgctat attcaatatc ttgcacaatg aaaatatata tatagaccag tataatggtg
    10081 atatcagaca atgcttgaca gatagtataa ttttataaat gatattatcg tgttaaatat
    10141 ttaagattaa cttacataaa agagattaag agatcacaaa gtataaacaa attttaatta
    10201 aaactaattt tttaagttaa aaaaaaatta atcatttttg agtaataaaa tcagtattat
    10261 acttatttat ttggtttata aaagggcgga gtaactactt tagccttggt taatttattt
    10321 ctaatattta taaatatttc agtatcagga tcagagaaat ctataggtac atatcctaat
    10381 ccaatagctt ttttcacata aggggcaaat gtaccagagg ttacattacc tataatgtct
    10441 cctttatcat tgcaaatagg ataatcatga cgagggatgc ctttgtctac catctcaaat
    10501 ccgattaatt tacgtttaat tccttgttgc ttttgtttta ataaaaactc tttatctatg
    10561 aagtttttat gatctacaaa tttaactatc catcccaaac ctgcctctaa tggggaagta
    10621 gtatcatcaa tatcattgcc atataggcaa aatcccattt ctaatctaag agtatctcgt
    10681 gcgccgagtc cagcaggtat aatttcttca tctttaccag cttcgaaaat agcttcccat
    10741 attattgtag catgtttggg atcaaaatat atttcacatc cgccactgcc cgtatatcct
    10801 gtagtagata gcaaaatatc tttaatatta gctatagtta catattgcca tgtataatgt
    10861 ttcatatcca ttataggctc ttctactatt ttttgcatta ttttcattgc taatgggcct
    10921 tgtatagcta gttgagctat attatcagat tcatttatca agtctttacc aatttcgata
    10981 ttaaattttg gagcatgaga gcatagccag ttccaatctt tttctatgtt agcagcattc
    11041 actactagca aatattcttg ttcattaatt ctatagacta ataaatcatc tactatgccc
    11101 ccctttccat taggaaagca agaatattga attttaccat ctttgagtgc tgatacatca
    11161 ttagaagtca catattgagc aaagtccaag gcattctggc cttttatata tatttctccc
    11221 atatgagaaa catcaaaaac tcccactcgc tctctaacag ctatatgttc ttcttgtatt
    11281 cctttatatt gcattggcat ataaaaacca gcaaattcta ccatcttggc tcccaaactt
    11341 tcatgaatct tagtaaatgg tgtgtacttc atatttctta attttttagc aaatttacaa
    11401 ttaataaatc aattacaaat atttttatta aaatttatta tatacaaatt aataacaata
    11461 aatctgtcac aaagtaactt ttatgctaat ttatataata agcaactttt atgcttatta
    11521 aaccttatta attgatcatt tattgcaaaa aaaattgaat ctattaattt aaatatatat
    11581 atctttaaaa aaatattttt atatacattt gcaagtatga aatatatttt atatccattt
    11641 gcttttattt tttatttaat acagaaaatc agaaaattct tatattcaat aaaaatttgt
    11701 aaaagatata ttccccctat cactaccatt aatattggta atttatcttt tggcggtaat
    11761 ggcaaaactc cacatactat ttatacggct cgattactat taaataataa ttataaagta
    11821 gcaatattat taagaggtta caaaagaaaa actagaggtt ttatagaagt aaatgatgat
    11881 gctagcgtcg ttgatgtagg tgacgaagca ttagtgtata aaagaaattt tggttcatta
    11941 gtaagagtag cagtatgtga gaatagagcc aaaggtatta gaattttatt agacaaatat
    12001 ccagacttag atattatttt actcgatgat gctatgcagc actggagagt aaattcacat
    12061 ttacaattat tactaacaga tttttctaat ccttttttta gagatcattt atttcccgtc
    12121 ggtcagctaa gagaacctag acgatcatct aaaagagctg atattataat aattacaaaa
    12181 ttcccttcta ttttttctcc cattattttt aaaaatttaa cagaaaaaat caaattgtat
    12241 ccgcatcagc aattatttgt cagctatatt agttataata aattaaaact tgtatttaat
    12301 aacaataaga atatagaaat atatgataca aaaccttatg ctatttttgt ttttactgct
    12361 atagccaacc cacaatcact taatgagcat ttacttttga tgtgtaaaga tttgataaga
    12421 tttaattttc cagatcatca tcaattcacg gaaaaagatg taataaaact aaaaaaggca
    12481 tttaatgatt atccttcagc aaataaaata atagtaacaa cagaaaaaga tatacccaga
    12541 ttagaactcc cctcaattaa aaaatattta ttagatttgc caatctatta tgcaccaata
    12601 gatataagat ttcataatat ggccaataat gagtataatt acgataaata tatattagat
    12661 tatgttagaa aaaatcaaaa aaacaacaga atttttaaag agtaaagtag atttaaaacc
    12721 tgaaataggt atagttttag gttcaggttt aggaaattta ggtaatgaga ttaatccagt
    12781 agcagtaatt ccctacaaag aaataccgaa ttttcctatt tctacagtaa tgggccatgg
    12841 cagtaattta atttttggca ctttagaagg taaaaatgta gtagctatgc aaggtcgctt
    12901 tcactattat gaaggttata ctatgcaaga gattactttt cctattagag tgatgaaggc
    12961 tctaggtgta caaagtttat tcctttctaa tgctgcaggt ggtatgaatc ccgattttga
    13021 agtaggagat ataatgctga tagtagacca tattaacttt atgggtacaa atccactaat
    13081 aggacccaat tatgatgaat taggtccgag attccctgat atgagtgagc cgtacagtaa
    13141 aaaatttatc aatttagcta agcaatgtgc taataactta aatataccaa ttaaagaagg
    13201 tgtttatgta gctgttacag gtcccaattt tgagacacca gcagaatata aattttttag
    13261 aattattggt ggagatgcag taggtatgtc tacagtacca gaagtaatag tggcagcaca
    13321 tagtgggttg cctgtatttg gcgtttctat tatcactgat ttaggaggtt ttgaagatat
    13381 acaacctatt tctcatgaag aggtattgaa agctgctgat gctgctgaac caaaactttc
    13441 agctatcata aaagcaatgt taaaagaatt gtaaagtaga ataacaaata gtatttttat
    13501 aaaactgatt tatcaaagca ctaaatttta aataaattaa tatgctatta aaagtttttt
    13561 ctgctgctat atttggtata gatgctatta tcgtaacagt agaagtggat ttagctcccg
    13621 ggactaaaac atttatttct ggtttaccag ataatgcagt aaaagaaagt cgtaatagaa
    13681 tagaacctgc catcattaat agtggttttc tatttccagg taaaaaaatc actattaatt
    13741 ttgccccggc agatattaga aaagaaggtt ctcattatga tttaccagta gctttaggaa
    13801 ttttgggtgc taataatcaa ttaccagatg tagattggtc tgagtatatg atagtaggtg
    13861 aattatcgct agacggtagt attctaccag tacacggtat cttacctatt gctataaaag
    13921 ctaaaagaaa taattttaaa aatttaatta tacctaaagc taatattaga gaagcatcag
    13981 taatagaagg attaaatatt tatggctttg ataatttgag agaagtaatt gatttttttc
    14041 atggtaattt tatacctgat aataataaaa ctgcaattaa agagtctata agtgaacata
    14101 catgtgaatc gataatagat ttcgctgatg tgaaaggtca agaaaatgta ataagagcta
    14161 tggaaatagc tgcagctggt cgtcacaata tattaatgat aggtccgccc ggagcaggta
    14221 aaactatgat agctagacgt ttacctacca ttatgccgcc aatgacattc gaagaaagta
    14281 tagaaactac tcaaatatat tctgttgccg ggaaaattgt aaataatggt ggattgataa
    14341 cagagagacc ttttagagct ccgcatcata ctattagtga tgtggcacta gtaggtggag
    14401 gtgctatacc taaacctggc gaaatatctc tagcacataa tggaatactt tttttagatg
    14461 aattgccaga atttaaaaga tcagtgttag aagttttgcg tcaaccactt gaagatcgcg
    14521 taattaacat aagtagagct aagtatgcta ctacatttcc tgcaaatttt atgcttgtag
    14581 cagctatgaa tccctgtcct tgtggatttt ataatcatcc agaaaaacaa tgtacctgtg
    14641 gacatggtgc aattaaaaaa tatttgaata aaatttccgg acctttactt gatcgtatag
    14701 atattcatat agaagtggta cctttggttt ttaaagaact tagttcttct aaaaccagtc
    14761 atagttcaaa agaaattaga aataatgcga tgcgtgcata tgaaatacaa aaagatagat
    14821 ataaaaattt gccaaaaatt tattcaaatg ctcaacttca ttcacgacta attaaaaaat
    14881 tttgtccagt aaaagatgaa ggtatggtat tattaaaaaa tgctatggaa aaattatcac
    14941 tctcagctag agcatatgat agaattttga aattagctcg tactatagca gatttagatg
    15001 aatctgaaat tattaaaccc tatcatctag cagaagctat acaatacaga actttagata
    15061 aaagtaattg ggggctatga aatcaataaa ttataataga tttgtttaaa tatgaatatc
    15121 aaactgaaac atttgtgtac atttattttc tcctaaaaga ttaacataac taataaaaaa
    15181 actcgcaaca ttaaaaaata ataatatgtt ttaaattaat tttttaagta acttataatt
    15241 tttgatttta actataatta cgaaaattta atattaaatt ttaaaaatta gtttttgaag
    15301 agtgggggag gtggggaatt tatatttttt tatgttaaat ttaatattgg cttagctctc
    15361 aagtccctgt ataaaaac
//
LOCUS       DAOPRN010000046        15089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_3341_length_15089_cov_97.342224, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000046 DAOPRN010000000
VERSION     DAOPRN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15089
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3341_length_15089_cov_97.342224"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..842)
                     /locus_tag="PKI65_06050"
     CDS             complement(78..842)
                     /locus_tag="PKI65_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOB78038.1"
                     /translation="MDSLFFYLSKIFDFVINPLFIVFLLLVIAIFSKKNKKLWVLVAV
                     ILLYIFSNPFLVTNVGRFWEIPKNPLPDSTYEIAIVLGGGIVTQTQDSNIVFMHNPDR
                     IMQALKLYNQGKVKKILLSSGSGSMIHRDILEAELLKQALVDIGYPDSIFIVESTSNN
                     TYENAVFSKNILDSLKIPYDKTILITSSMHFRRAYKCFLKQGIDCIAYPVAPIKSLQA
                     ISPSFYFLPDASALGVTNNLFHEVLGLIVYKIMGYA"
     gene            complement(906..1859)
                     /locus_tag="PKI65_06055"
     CDS             complement(906..1859)
                     /locus_tag="PKI65_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOB78039.1"
                     /translation="MIHKRILVTGGAGFIGSHLCERLLNEGNEVICLDNLFTSSKDNI
                     AHLLSNKYFEFIRHDIIHPIYLEVDEVYNLACPASPIHYQYNSIKTIKTSVMGAINML
                     GLAKRVKGKILQASTSEVYGDPLIHPQDEEYWGNVNPIGPRSCYDEGKRCTESLFINY
                     YFQNKVRIKIARIFNTYGPRMHPNDGRVVSNFIIQALQNKPITIYGDGNQTRSFCYVD
                     DMIDGLIKLMDTNDHITGPINLGNPDEFKIIELAEMIIKLTNSKSKIVYQPLPEDDPI
                     RRQPDITKAKTILNWEPKIHLKTGLIKTINYFENIIKNNVF"
     gene            complement(1945..3267)
                     /locus_tag="PKI65_06060"
     CDS             complement(1945..3267)
                     /locus_tag="PKI65_06060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOB78040.1"
                     /translation="MKIVIVGTGYVGLVTGVCFAEVGVDTVCVDIDKDKIDKLKKGLS
                     PIYEPGLDELITKNLEKGRISFDTDLANVIDGAEMVFIAVGTPPDEDGSADLKYVLDV
                     AHSIGCYMNDYLVVVTKSTVPVGTAQKVKEAISYELDKRGVDIEFDVASNPEFLKEGD
                     AINDFMRPDRIVIGVESDRAQEIFQRLYKPFTLNGHPLIFMDIPSAEMTKYAANAMLA
                     TRISFMNDIANLCEILGADVNMVRLGIGSDPRIGNKFLYPGIGFGGSCFPKDVKALIK
                     SGLENQYPLRLLQAVENINEDQKSILVKKLIKFYNGDISDKKIVLWGLSFKPNTDDMR
                     EAPSLVIIDKLLEKNAKVIAYDPVSIDEAKRRLGDKIEYANDMYEPLNDADALLLVTE
                     WTEFRLPDWDLVKKLMKTPLVLDGRNIYDAREMKQKGFFYYCIGVRTY"
     gene            3561..4277
                     /locus_tag="PKI65_06065"
     CDS             3561..4277
                     /locus_tag="PKI65_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB78041.1"
                     /translation="MKKSLLGILLFLFSFILLSQNSFRLYHQGMPLINHDSLYFYLDS
                     SQYTVEYMLLGIENISNTDKNVKVKKNEILIVPGSENTFCWGMCYTSSVYISTKYITI
                     QAHTTNNTDFYGEYYPKGNLGTSIIMYTFFDINNPDDSVSVVAFYIATPVNTENFDLC
                     NNYSLYPNPSSGKIYLKKSFTNEAIITIYNPYGIKVYESLIENNEIDISMLSDGLYIY
                     RILVDGEIKGSDYLLIRSNK"
     gene            complement(4325..6526)
                     /gene="rnr"
                     /locus_tag="PKI65_06070"
     CDS             complement(4325..6526)
                     /gene="rnr"
                     /locus_tag="PKI65_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOB78042.1"
                     /translation="MTKKQPKKNKTKNIKDNLEKIEQEPLLANILAIFKQSVDESFNY
                     KQISAQLGYTNLEDRQKVAKGLEKLCRLGALIRVGRGKYQLAPQIIEKEAPPSVLIGK
                     LDFKSTGKAYFINPDLNEDIFIAAPNTGQALPGDKVKIRLLPKRKNRKPEGIVIEVLE
                     RNRNQFVGVFEKGEYAGYVIPDDSRMPVEFVIPKELINGATEGDKVVVELVEWEKGIR
                     SPVGKIIRVLGQPGENEVEMLSILVNNGFHLDFPEDVLQESLDISKEIKEADIKYRKD
                     FRDVLTFTIDPAEAKDFDDAISFKKLDNDTYEIGIHIADVTHYVKPQSKIDKEAYQRA
                     TSVYLVDRTLHMLPERLSADVCSLKPGEDRLAFSVILKMNSKAKVLSSDFYETVINSD
                     YRFNYEEVQQIIEGKRDDVNHDALRTLNDLAQILRADRFRRGAINFHSEEINFILNEQ
                     KKPIDIVTKEQKEANQLIEELMLLANKTVAEYIGLILKQKFNRELPFVYRVHDKPSAE
                     KIANFAVFLRKLGYKLNLENEVALAKSMNHLFEQVADRPEENMINQIAIRTMSRAIYT
                     THNIGHYGLAFKYYTHFTSPIRRYPDIMVHRLLKSYLAKNNKIRYEKISELEEKCKHA
                     SEMEEKAQNAEWDSIKYKQAEFMLDKVGKVFTGYITGVSKWGLFVELDGKKCEGLVHM
                     VDMKDDYYYLDENNYMLVGHRTGIKYKLGDRVRVKIKYVDVAKKQIGMTLC"
     gene            7134..8648
                     /locus_tag="PKI65_06075"
     CDS             7134..8648
                     /locus_tag="PKI65_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOB78043.1"
                     /translation="MKLVKKDIFPYIYLVLISLLILGIFYSLFLISVSEITLALVWLI
                     EGNYKDKLSKLKKQPYVILWISLYIFLIIGLFYSTDLKYGFNDLRVKLPMLLLPLAFA
                     TITPHDFLYKYFNKFYLFSSLIFVLIPIIAYITGLIASVKDISWYQNHIRASLLITIA
                     IAIILFSPETIKFKKNRIVIPILIILFIFFQILIASLAPFLLSIMIFIIWLIIKIYTK
                     NKYIAITFLLLLLIGLPLGMFFWYQSFFGIPKQISKNDIKTLEIENKNLFNPYLIDES
                     LVIAWNEVSEKSINENNPLADITYYTAVRFLASKNLPLDYRGIKMLSKEEIKAIEQGI
                     PNYRLFNTIPIITRVYNLFYEIYRINKDGQLYFYGSLSQRLIYLQAGISLIKEKPLFG
                     WGTGDVNLAFTKYYSKNYPEISPKLYRKAHNQFVTIGVALGLAGMLLLFSLWLYPIFN
                     TKLRKNISSLWICLWVIFTVMMLIEDMLEGQQATTMTVFFLNFYLLLFKNPSKI"
     gene            complement(9302..10150)
                     /locus_tag="PKI65_06080"
     CDS             complement(9302..10150)
                     /locus_tag="PKI65_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOB78044.1"
                     /translation="MSWKEVRSYLLITIGLVLNAIGWNAFLIPSEIIGGGLVGIASIL
                     WFTLKIPIGLTVLVVNAILVVLAIKLVDASFGIKTIVGIIINVIALSIFQLLFKEPLV
                     TDKFMAAIIGATLCGTGLGVVFTQGSASGGTDIIALIINYYKNITPGKVILLFDIVII
                     GSTYFIFHSIEKVMYGYVVMAVTSYVIDLVMEGNKQSYQFMIFSKKNKEIADRIIKEV
                     GRGVSVLKGLGWYTKEPMDVLIVICRRTDRNHITSIINEEDPKAFFSITKVSAAYGLN
                     FERVKF"
     gene            complement(10255..11682)
                     /locus_tag="PKI65_06085"
     CDS             complement(10255..11682)
                     /locus_tag="PKI65_06085"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOB78045.1"
                     /translation="MKKIKSIYLTILLLFILLIISCHPEREYDRYSLLKFSSDSVFID
                     TSFATVTTITRIIKVYNTSSKPILIDKVYLSNGSNSMFRFNIDGEPGPIVIDLEIDAK
                     DSLFLFINATIDPNDANTPFIIEDSIIFIVNGKNQKIKLAALGQNAYFHTPDHPPTQY
                     LPAYSLVSGVWENDKPHVIYGIAVVDEDCTLVIPAGTKVYMHRNAMLWVYKGGSLKIK
                     GNPFEPVLITSDRLDPFYRDQAGGWDRIWLSALSKDNEIDWAIIQNGNVGIHADTVAN
                     ANPTLKINNTIIRNMSTACLFAQGAKIVATNCLFANAKYYSALLSIGGYYVFKHCNFV
                     NFWNTSHRSSSLLALNNYYKDINGNIQIRPISVYMGNCIVYGDQEEEILIDKHPGIDN
                     FFLTLDHCLLRTKYNLGDSIFINCIRNKDPEFRNHSIGDFRLQNNSPCIDAGKINISW
                     GINNDLSGGPRIANGVPDIGAYELY"
     gene            12038..13066
                     /locus_tag="PKI65_06090"
     CDS             12038..13066
                     /locus_tag="PKI65_06090"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOB78046.1"
                     /translation="MDTSKIKWAELPFGYFKTDYNIRCYYKNGSWSPIEVSDSEYFPI
                     HIAATCLHYGQEIFEGLKAYRGKDGKIRLFRWDENAKRLQYSAQGLVMAEVPEDLFFQ
                     AIKEAVLRNEKYVPPYGTGATLYIRPLEIGISAEVGVKASKDYMFIVFVSPVGPYFKT
                     GFKPTKIAIERDSDRAAPLGTGTYKCGGNYAASLRGTIRAAKFGAGSPMYLDPKEKKY
                     IDEIGAANFFGIKNNTYITPESSSILRSITNKSLQQLAKDMGLKVECRPVPLEELPTF
                     EEVGACGTAAVITPIGEIIDMSTGETYNFCPDGKPGPISTKLYNTLTGIQFGDIPDKH
                     NWITILND"
     gene            complement(13150..14610)
                     /locus_tag="PKI65_06095"
     CDS             complement(13150..14610)
                     /locus_tag="PKI65_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOB78047.1"
                     /translation="MNNPFKNLEPQKVWQYFYEITQIPRPSKHEEKIIEYLENFAKAK
                     NLEYKKDRVGNIVIRKPASKGGENKPMVALQCHVDMVCEKNADVEKNFLIDPIEPYID
                     NEWVKAKGTTLGADNGIGVAMQLALLDSDIEGLGPIECLFTVDEETGLTGANALENDM
                     IKSKYLINLDSEDEGQIFIGCAGGKNTIGYLPIKYQSGEEKYSAEIFVSGLQGGHSGD
                     DIEKKRGNAIKLITRIIYDLLVNYENIQIIDIEGGNLHNAIPREAKAIITFDTDKDFN
                     KIASIVEKYQNIFKTELKISDPDVKVSISKKGKTNKWLDSELANRIIRLLYAIPHGVF
                     AWSQDIPDFVETSTNLASIKRQNNEYMITTSQRSSLESAKENIGNKVKSAFELADAKV
                     EVTDGYPGWTPNPESFLLKIVAEETEKVLNKKPIIRAIHAGLECGLFSEKFPWLEMVS
                     IGPEMKGVHSPDEKLLIPSVKRTFDILINVLKKIDK"
     gene            14708..>15089
                     /locus_tag="PKI65_06100"
     CDS             14708..>15089
                     /locus_tag="PKI65_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78048.1"
                     /translation="MKTSKFLSILVIAFSLISNANAQTNLAAWHFDVLAAAPNTPKII
                     QADYGLQSNSATIYLDGTHGSSDWNSSTTNPELTAFVGSTTNDQRPSPLAGQALALAN
                     SSANNKGVVFALSTENYENIKISYA"
BASE COUNT         5085 a         2362 c         1951 g         5691 t
ORIGIN      
        1 tctttgtggt taaaaaataa cctatttgta acaatctaaa atttgattac ccacactatc
       61 cgttatagaa ctaaatatta tgcatacccc attattttgt aaactattaa gcctaagact
      121 tcatgaaaaa gattattagt tacccctaat gctgaagcat caggcaaaaa ataaaaagaa
      181 ggagatatag cttgtagaga tttgattgga gctacaggat aagcaatgca atctattcct
      241 tgctttaaaa aacatttgta tgctcttcta aaatgcatag aagatgtgat cagtatagtc
      301 ttgtcatagg gtatttttaa agaatctaaa atattttttg agaaaactgc attttcataa
      361 gtattattag atgttgattc tactataaat atcgagtcgg gatagcctat atctactaat
      421 gcttgtttta acaattcagc ttctaatata tctctatgta tcatagaacc actgccacta
      481 cttaaaagta ttttttttac cttaccttga ttgtacaatt tcagtgcctg cattattcta
      541 tcaggattat gcataaaaac gatattacta tcttgagttt gagtaactat gccaccacct
      601 aaaactatag ctatctcata agtgctatca ggtaatggat tttttggtat ttcccaaaat
      661 ctaccaacat tggtaaccaa aaaaggatta gaaaaaatat aaagtaatat cacagcaacc
      721 aaaacccata attttttatt tttttttgaa aaaatagcta ttactaaaag taaaaaaact
      781 ataaatagag gattgattac aaagtcaaat atttttgaaa gataaaaaaa taatgaatcc
      841 attccgattt attaattatt ttaataaaag acgataaaaa attatttttg aattgttata
      901 ttttttcaaa atacattgtt ttttataata ttttcgaaat aattaatagt ttttattaag
      961 ccagttttca gatgtatttt aggttcccaa ttgaggatag ttttagcttt agtgatatca
     1021 ggttgccttc gaataggatc atcttcgggc aaaggctgat aaacgatttt tgatttagaa
     1081 tttgtcagct ttataatcat ttcagctaat tcaattattt taaattcatc aggatttcct
     1141 agattgatag gaccagttat atggtcatta gtgtccatca acttgataag tccatctatc
     1201 atatcatcta cataacaaaa actacgtgtt tgattaccat ctccataaat agtgataggt
     1261 ttattttgca aagcttgaat tataaaatta gatactactc ttccatcatt tgggtgcata
     1321 cgaggcccat aagtattaaa aatacgagca atttttattc ttactttatt ttgaaaataa
     1381 taatttataa acaacgattc tgtacatcgt ttgccttcat catagcaaga gcgtggtcca
     1441 ataggattta cattacccca atattcctca tcctgaggat gtatgagagg gtcaccatat
     1501 acttcactag tgcttgcttg tagtatttta ccttttactc ttttggctaa accaagcata
     1561 tttatagctc ccattacaga ggtttttata gttttaatag agttatattg ataatgtatt
     1621 ggagatgctg gacaagctaa attataaacc tcatctacct ctaaatatat aggatgaata
     1681 atgtcgtgtc taatgaattc aaaatattta ttagaaagta aatgagcaat gttatctttt
     1741 gatgaagtaa acaaattatc taaacatata acttcattac cttcatttaa taatctttcg
     1801 cataaatgag aacctataaa tccagcccca ccagttacta atattctttt atgtatcatt
     1861 tatttatttt tttaaaatta aaacctacaa cttatctatt ttgatcttat tccgctgtag
     1921 tctctatcta actttcataa aattttaata tgttctaaca cctatacaat aataaaaaaa
     1981 tcctttttgt ttcatttctc ttgcatcata aatatttcta ccatcaagta ctaatggagt
     2041 tttcattaat tttttaacta aatcccaatc tggcaaccta aattctgtcc attctgttac
     2101 tagcaacaga gcatctgcat catttagtgg ctcatacata tcattagcat attctatttt
     2161 gtctcccagc cttcttttag cttcatctat actaactgga tcataagcta tcactttagc
     2221 atttttttct aatagtttat ctataataac taaagatgga gcttctctca tatcatcagt
     2281 attaggctta aaagataatc cccacagtac aatttttttg tcagatatat cgccattata
     2341 aaatttaatt aattttttta ctaatatact tttttgatcc tcattaatat tttcgacagc
     2401 ttgaagtaga cgtaggggat attgattttc taatccagat tttataagtg ctttcacatc
     2461 ttttgggaag cagctaccac caaagccaat acctgggtat aaaaatttat tacctattct
     2521 cggatcagag cctatgccta gtctaaccat attaacatcg gcacctaata tctcacaaag
     2581 attagcaata tcattcataa aactaattcg tgtagctagc atagcattgg cagcatattt
     2641 agtcatttct gccgaaggga tatccataaa aataagtgga tgtccattta gagtaaaagg
     2701 cttgtataat ctttgaaaaa tctcttgagc tctatcagat tcgactccaa taactatacg
     2761 atctggtctc ataaaatcat taattgcgtc accttctttt aaaaattcag gattagacgc
     2821 aacatcgaat tctatatcta cacccctttt atcaagttca taggatatag cttctttaac
     2881 tttttgggct gtacctactg gtacagtact tttagtaact actacgagat aatcattcat
     2941 ataacaacca atagaatgcg cgacgtctaa aacatatttg agatctgcac taccatcctc
     3001 atcaggaggt gtacctactg ctataaagac catctcggct ccatctatta catttgctaa
     3061 atctgtatcg aagcttattc ttcctttttc taaatttttt gtaattaact catctaaccc
     3121 cggttcgtat attggagata atcctttttt taatttatct attttatctt tatcaatatc
     3181 tacacaaaca gtatctacac ctacttcagc aaaacataca cccgttacta aacctacata
     3241 tcctgtccct acaattacta ttttcataaa attatttttt tgcaaaataa ataatttttt
     3301 agaaactata aaattatctt tcgcatataa ctaaataact aacaaggaga aaagatgatg
     3361 aggtgaaagg tgaaatgata acaagaaaaa gtggatacaa ggtttagaca tttaatattt
     3421 tagaattttt tgtaaagttt ctttttgtat ttataaataa tatccttaga atatctattg
     3481 aatatattga caagctatta aaaataataa tattattgta aaatatttga aaatatctat
     3541 atatttgtaa aaaatttatt atgaaaaaat cattattggg aattttatta tttcttttct
     3601 cttttatatt attatcacaa aattcattta gactatatca tcaaggaatg cctttaataa
     3661 atcatgactc tctgtatttt tatttggata gtagccaata cacagtagaa tatatgcttt
     3721 taggtataga aaatattagc aatacagata aaaatgtcaa agtaaaaaag aacgaaatat
     3781 taatcgtacc aggatcagaa aatacttttt gctggggtat gtgttataca tcatcagtat
     3841 atatttctac taaatatata accatacaag ctcatactac aaataatact gatttttatg
     3901 gtgaatacta cccaaaagga aatttaggta cttctattat aatgtatact ttttttgata
     3961 taaataatcc agacgatagc gtcagtgtag ttgcatttta tatagcaacg cctgttaata
     4021 cagaaaattt tgatttatgt aataattatt ctctatatcc caatcctagt tctggcaaaa
     4081 tataccttaa aaaaagtttt acaaatgaag caataataac aatttataat ccttatggta
     4141 ttaaagtata tgagagttta atagaaaata atgaaataga tatatctatg ttatcagatg
     4201 gattatacat ttatagaatt ttagtagatg gtgaaataaa aggatcagac tatttattaa
     4261 tacgaagtaa taaataaaaa attttgttct tttttagggg gagaagtgct aaaaaaatta
     4321 tatatcaaca caaagtcatg ccaatttgtt ttttagctac gtctacatat tttattttta
     4381 ctcttactcg gtctcctaat ttgtatttaa tacccgtacg atgtcctaca agcatataat
     4441 tattttcgtc taaataataa tagtcatcct tcatatctac catatgcacc aaaccttcac
     4501 attttttacc atctaattct acaaacaatc cccacttgct cacaccagtt atatatcctg
     4561 taaatacttt acctacttta tctaacataa actctgcttg tttatacttg atagaatccc
     4621 attctgcatt ttgagctttc tcttccattt cacttgcgtg tttacacttt tcctctaatt
     4681 ctgatatttt ttcatatctt attttgttgt ttttagctaa atatgacttt aataaacgat
     4741 gaaccatgat gtcaggataa cgacgaatag gcgaggtgaa atgagtataa tatttaaagg
     4801 ctaaaccata atgccctata ttgtgtgtag tatatatagc acgagacatt gtacgtattg
     4861 ctatctgatt gatcatattc tcttcgggtc tatctgctac ttgctcaaat aaatgattca
     4921 tagatttagc tagagctact tcattttcca ggttcaattt atatcctaat tttcttaaaa
     4981 aaacagcaaa attggcaatt ttttccgctg aaggcttatc atgaacacga tacacaaaag
     5041 gtaattctct attaaatttt tgttttaata ttaaacctat gtattctgcc acagttttat
     5101 tagctaaaag catcaattcc tctattaatt gattagcctc tttttgttct tttgtgacta
     5161 tatcgatggg ttttttttgc tcattcaaaa taaaatttat ctcttcagaa tgaaaattta
     5221 tagctccacg tctaaagcga tctgcacgca agatctgagc caaatcattt aatgtgcgta
     5281 aggcgtcatg gtttacatca tctcttttac cttctattat ttgctgcact tcttcataat
     5341 taaatctata atctgaatta ataacagttt cataaaaatc actacttagc acttttgcct
     5401 tactattcat ttttaaaatt actgaaaatg ctaatctatc ttcgccaggt ttcaatgaac
     5461 acacatcagc agacagtctt tcggggagca tgtgaagtgt tctatccact aaatataccg
     5521 aagtagctct ttgatatgct tctttatcaa ttttgctttg tggctttaca taatgagtga
     5581 catcagctat atgtatacct atctcataag tatcattgtc caattttttg aatgaaatag
     5641 catcatcgaa gtctttagct tcagcgggat ctatcgtgaa agtaagcaca tcacgaaaat
     5701 cttttcggta ttttatgtcg gcttctttga tttcttttga tatgtctaag gattcttgca
     5761 aaacatcttc aggaaaatct agatgaaatc cattgtttac tagtatagac agcatctcta
     5821 cttcattttc accaggttgt cctaatacac gtattatctt acctacagga gatctaattc
     5881 ctttttccca ttctactaat tccaccacta ctttatcacc ttctgtagct ccatttatta
     5941 gttcctttgg gattacaaat tctactggca tcctggagtc gtcaggtatc acataaccgg
     6001 cgtattcgcc tttttcgaaa acccctacaa attgatttct attgcgttct aacacttcta
     6061 ttactatgcc ttcgggcttt ctattttttc gttttggcaa tagtcttatt tttactttat
     6121 cacccggaag tgcttgtcca gtatttggag cagctataaa aatatcttca tttagatctg
     6181 ggttaataaa ataagcctta ccagtacttt tgaaatctaa tttacctatt aaaacactcg
     6241 gaggggcttc tttttctatg atttgggggg ctaactgata tttgcctctg cctacacgaa
     6301 ttaatgctcc taatctacat aatttttcta atcctttagc tactttttgc ctatcttcta
     6361 aatttgtata gcctaattgt gctgatattt gcttataatt aaaactctca tcaactgatt
     6421 gtttaaatat tgctaagata ttagctaata atggctcttg ttctatcttt tctagattat
     6481 cttttatatt ttttgtttta ttctttttgg gttgtttttt agtcataatt caaataaaag
     6541 ttttgtattt tttgcaaaat taatttaatc tattaattta tacgtatcta attgtataaa
     6601 aaaatcaatc atccttttat aaaaaaaacc tcaattactt caattttttt ttaatctctg
     6661 tcataaaatc taaaccaaaa tatcaaagtt ttattacatt ggtaaaaggg atgtttgtaa
     6721 acaaaaattt tgtcgtactt aaatttttta tggcaaattt aagcattttt tgtaaattat
     6781 tcttaatatt aattttttat ctaaatatca aaattgaata tataattaac aatgatttta
     6841 gattaattac acttttttta aattattatg ttctcactga taattttttt ttgatatagt
     6901 ataaaaattg ggactttcat taatggaaat acttgagcaa ataacctaaa tttataaatg
     6961 ttatttttac tgatgatttt tatgtatttt tgtttaaaaa gaaattaaaa atcatattta
     7021 aaaaaattga aatcaaactt tattttttac attttgatta tttttggttt ttttaaagta
     7081 taacaataat gatatacttt ttttattaaa aaaaatatga ataataagca tatatgaaat
     7141 tagttaagaa agatattttt ccatatattt atcttgtttt aatctcctta ctaatattag
     7201 gtatttttta ttcattattt ttaatttctg tatctgaaat tactttagct ctagtgtggc
     7261 taatagaggg caattataaa gataaattaa gtaaactcaa aaagcaacca tatgtaattt
     7321 tatggatatc cttatatatt tttttaatta ttggtttgtt ttattctact gatctcaaat
     7381 atggctttaa tgatttacga gtaaaattgc ctatgcttct actaccatta gcttttgcta
     7441 ctatcacacc acatgatttt ttatataaat attttaataa attttattta ttttcatcat
     7501 taatttttgt attaatacca ataatagctt atattacagg cttgatagct agcgtaaaag
     7561 atatatcttg gtaccaaaat cacattagag cctcattatt gatcactatt gctattgcta
     7621 ttatattatt ttcacctgaa actattaaat ttaaaaaaaa tagaattgtt attccgattt
     7681 taattatttt atttattttt tttcaaatat taatagcatc attagctcca tttcttcttt
     7741 caataatgat ttttatcatt tggctaatta ttaaaatata tacaaaaaac aaatatatag
     7801 cgataacttt tttgttatta ctattaattg gtttaccatt aggtatgttt ttttggtatc
     7861 aaagtttttt tggtatacct aaacaaattt ctaaaaatga tataaaaact ttagaaatag
     7921 aaaacaaaaa tctatttaat ccatacttaa tagacgaaag ccttgtaata gcttggaatg
     7981 aagtgagtga aaaatctata aatgaaaata atccattagc tgatatcacc tattatacag
     8041 ctgtcagatt tttagcttct aaaaatttgc ccttagatta tcgtggcata aaaatgctat
     8101 caaaagaaga aataaaagca atagagcagg gtatacctaa ctataggctt tttaatacaa
     8161 taccaataat cactcgcgta tataatctct tttatgaaat ttatagaatt aataaagatg
     8221 gacaattata tttttatggt tcactaagtc agcgattaat atatttacaa gccggtatat
     8281 cattaataaa agagaaacca ctttttggct gggggacagg agatgtaaac ctagcattta
     8341 caaaatatta ttcaaagaat tatcctgaaa tatcacctaa attatatcga aaggcacaca
     8401 accaatttgt tactatagga gttgcattag gactagctgg tatgttatta ttgttttctt
     8461 tgtggttata tcctattttt aatactaaac ttcgcaaaaa tatttcaagt ctatggatat
     8521 gtctgtgggt tatttttact gttatgatgc taatagaaga catgctcgag ggtcagcagg
     8581 ctactactat gacagtattt tttttaaatt tttatttact tttatttaaa aatccttcaa
     8641 aaatttaacg attttatttt aaaaaaataa ctttattcaa aaaaaaatat tgttttagaa
     8701 aatggtactt ataatttttt gacttcataa gaatgatttg ttggaagggg tttcatgtaa
     8761 tttatttttg ttatacctta agtattttat acgattattt ggtaattaga cgcaatgtat
     8821 atagtctaaa ggttgtcgat aagaatataa atgatttcaa gatttagctt aggccattta
     8881 gaagagtagt taccaaatac ttacagagct atttaaatgt gagatataat taaaactact
     8941 ttaagtataa caattattaa tttcaatatt tttttgtttc tccactacta acccaattag
     9001 atttttatta aaattaaatc taatcatact ttgaataaga tgtcaactat gtatactaca
     9061 ccgtactaga tgaaataata aaataaagaa ttggaaatga gggaatagaa atacaggaag
     9121 caaataaata tatcattaat tgtaagtcat taactattga agccatataa tgtctatatt
     9181 gtgtctttgt aatagttatt gtaaaaagtt gtatttttat aaacctcgcc acaaactttt
     9241 aaaagttaat attttaatca ttgaagcaaa cgaatagatt taatatttga aaaatatcat
     9301 tttaaaattt tacacgttca aagtttaatc cataagcggc tgatactttc gtgatagaga
     9361 aaaatgcttt tgggtcttct tcattaataa tacttgttat gtgatttcta tctgtacggc
     9421 gacatataac tattaaaaca tccataggtt ctttagtata ccatcctaaa ccttttaata
     9481 cactaacacc tctacccacc tcttttatta ttctatcggc aatttcttta tttttctttg
     9541 aaaaaatcat aaattgataa ctctgcttat taccttccat tactaaatct attacataag
     9601 atgttacagc catcactaca taaccataca ttactttttc tatactatga aatataaaat
     9661 atgtggaacc gattatcact atatcaaata ataaaataac cttgccaggt gttatatttt
     9721 tataataatt tatgatcagt gctattatgt ctgtaccacc gctagcacta ccttgtgtaa
     9781 atactacacc taatcctgtc ccacacaagg tagctcctat aatagctgcc ataaatttat
     9841 cagttactaa tggttctttg aataaaagtt gaaatataga taatgctatt acatttatta
     9901 taatccccac tattgttttg ataccaaaac ttgcatctac gagcttgatt gctaagacta
     9961 ctaatatcgc attgactacc aatactgtta atccaattgg aattttgaga gtaaaccata
    10021 gtatactagc tatacctact aatccacctc ctattatttc tgatggtatc aaaaatgcat
    10081 tccaccctat agcatttaat actaatccta ttgttattaa taaatatgaa cgcacttctt
    10141 tccaagacat aattttactt attttttatg aatgacaaaa ataaataaaa tattttacta
    10201 cacttaatta taaattaatg tgatttttat gagaaaatat atcaaccaaa acaattaata
    10261 taactcgtaa gctccaatat caggaacgcc atttgctatt ctagggcctc cagagaggtc
    10321 attattaata ccccaagaaa tatttatctt gccagcatct atgcaaggag aattattttg
    10381 taatctaaaa tcaccaattg aatgattacg aaattcagga tctttatttc tgatgcaatt
    10441 aataaatatc gaatcaccta aattatattt agtgcgcaat aaacaatgat ctaaagttag
    10501 aaaaaagttg tctattccgg gatgtttatc tattaaaatt tcttcttctt gatctccata
    10561 cacaatgcag ttgcccatgt acacgcttat aggacgtatt tgtatatttc catttatatc
    10621 tttataataa ttatttaatg ctaaaagtga gctagatctg tgagacgtat tccaaaaatt
    10681 aacaaaatta caatgtttga acacataata accaccaata gacaacaaag cggaatagta
    10741 tttagcattt gcgaaaaggc aatttgtggc aactattttg gctccttgag caaatagaca
    10801 cgcagtagac atattgcgaa taatagtatt attaattttt agagtaggat tagcattagc
    10861 tacagtatct gcatgtatac ccacattgcc attttgtatg atagcccaat ctatctcatt
    10921 gtctttacta agagctgata accatattct atcccagcca ccagcttgat ctctatagaa
    10981 aggatctaat ctatcactag taattagcac aggctcaaaa ggatttcctt ttatttttaa
    11041 actaccacct ttatacaccc aaagcatagc atttctatgc atatatactt tagtaccggc
    11101 aggtatcact agagtgcagt cttcgtctac tacagctata ccatagatta cgtgaggctt
    11161 atcgttttcc catactcccg aaaccaagct ataagctggt aaatattgtg taggggggtg
    11221 gtcaggagta tgaaaatatg cgttctgacc aagggccgct aatttaattt tttgattttt
    11281 accatttaca atgaaaatta tactatcttc tattataaat ggtgtattag catcattagg
    11341 atctatagtg gcattaataa ataaaaataa gctatcttta gcatctattt ctaaatcaat
    11401 aactattgga cctggctcac cgtctatatt aaatcgaaac attgaattgc taccattact
    11461 taaataaact ttatctatta atataggttt agatgatgta ttatatactt ttataattct
    11521 cgtaatagta gtgactgtag cgaaactagt gtctataaaa acactatctg acgagaattt
    11581 taataaacta tatctatcgt attcacgctc aggatggcaa gaaattatta aaagaataaa
    11641 aagtaataag attgttaaat atatgctttt aatttttttc atttttttat ataatatttt
    11701 aaacaaaatt aaatcattaa tattaattct aaatattctt ttttttttaa ttttactcat
    11761 ttataatttt aaaacaatta aaaaaaatta tgcttttaaa aaatattaca aagataaaaa
    11821 aaatctaaat ttttataatc acttttgcct tcacctaaat attctatttg agcttttata
    11881 gttaattgta aattattagc aattttttta tacattttta tcaaatacaa aatttatttt
    11941 gatttaaatc aaaataaatg tgtaattata tcaattatat tattttataa atattatttt
    12001 tatgtaaata attatttata acttagcaaa aaaaaatatg gatacatcaa aaattaaatg
    12061 ggctgaattg ccatttggtt attttaaaac cgattataac attaggtgct actacaaaaa
    12121 tggaagttgg tcacctatag aagtgagtga ttcggaatat tttccgattc acattgcagc
    12181 tacgtgttta cactatggtc aagagatatt tgaaggctta aaagcatata gaggcaaaga
    12241 tggtaaaatc agattattta gatgggatga aaatgctaaa cgtttacaat acagtgctca
    12301 aggattagtt atggcagaag tcccagaaga tttatttttt caagctatca aagaggctgt
    12361 attgagaaat gaaaaatatg tacctcctta tggtactggt gctactttat atatcagacc
    12421 tcttgagata ggtattagcg ctgaagtggg agtgaaagct tcaaaagatt atatgtttat
    12481 cgtatttgta tcaccagtag gtccatattt taaaactggc tttaaaccta ctaaaatagc
    12541 tattgaaaga gattcagata gagcagctcc attgggtact ggtacttata aatgtggtgg
    12601 taattatgca gctagtttac gtggtactat tagagctgca aaatttggtg caggctctcc
    12661 tatgtatctt gatcctaaag aaaaaaaata tatagatgag ataggagctg ctaatttctt
    12721 tggaattaaa aataatactt atattactcc agaaagttct agtattttgc gttccataac
    12781 taacaaaagt ttacagcaac ttgctaaaga tatggggtta aaagtagaat gtagacctgt
    12841 acctcttgaa gaattaccta ctttcgaaga agtaggtgct tgtggtacag cagcagttat
    12901 cacacctatt ggtgaaatta ttgatatgtc tacaggtgaa acttataatt tttgcccaga
    12961 tggtaaacca ggacctattt ctactaaatt atataatact ttaactggta ttcaatttgg
    13021 agatattcct gataaacata actggattac tattttaaat gattaatagt tacattttaa
    13081 aaaaaataga ggttcaaata tttgaacctc tatttttttt aaaattttgt gtagattatc
    13141 tttttttgat tatttatcta tcttttttag tacatttatc agaatatcaa aagttctttt
    13201 tacgcttggt attagtaatt tctcgtcagg actgtgtacg cctttcatct cggggcctat
    13261 tgataccatt tctaaccaag gaaatttttc actaaaaagt ccacattcca gtccagcatg
    13321 tatagctcta attataggtt ttttattcaa aactttttca gtttcctcag ctactatttt
    13381 tagtaaaaaa ctttctggat ttggcgtcca tcctggataa ccatcagtta cttctacttt
    13441 tgcatcagct aattcaaaag cagattttac tttattgcca atattttctt tagcagattc
    13501 taatgagcta cgttgactag tggtgatcat gtactcattg ttttgtcttt ttatacttgc
    13561 taggttggtg gaggtttcta cgaaatctgg aatatcttgt gaccatgcaa aaactccatg
    13621 aggtatagca taaagcaaac gtattattct gtttgccaac tcactatcta gccatttatt
    13681 agtttttccc ttttttgaaa tagatacttt gacatcgggg tcactaattt ttaattctgt
    13741 tttaaaaata ttctgatatt tttctacaat gcttgcaatt ttattaaaat ccttatcagt
    13801 atcaaaagtt ataattgctt tagcttctcg tggtatagca ttatgcaaat tgccgccttc
    13861 aatgtctata atttgtatat tttcatagtt tactaacaaa tcatagatga tacgtgtaat
    13921 tagtttaata gcgttaccac gtttcttttc tatatcatct ccagaatgtc ccccttgcaa
    13981 tccagaaaca aaaatttctg cactatattt ttcttctcca ctttgatatt tgataggtaa
    14041 gtagccaatg gtatttttac caccagcaca acctatgaaa atttgtcctt catcctcact
    14101 atctagattt atcaaatatt tactttttat catatcattt tctagtgcat tagcaccggt
    14161 aagtccagtt tcttcatcta ctgtaaaaag acattctata ggacctaacc cctcgatatc
    14221 actatctaga agcgctagtt gcatagctac ccctatgccg ttatctgcac ctagagtagt
    14281 gcctttagct tttacccatt cattatctat gtatggttct attggatcga ttaaaaaatt
    14341 tttttctaca tcagcattct tttcacacac catatctaca tgacattgca gagcaaccat
    14401 aggtttgttt tccccacctt tgctggctgg ctttcgtata actatatttc ctactcggtc
    14461 ttttttatat tctaagtttt tagctttagc aaaattttct aaatattcaa ttattttttc
    14521 ttcatgttta gatggcctgg gtatttgcgt gatttcataa aaatactgcc atactttttg
    14581 tggttctaaa tttttaaatg gattattcat aataaatata ttttttgcta aaatatggtt
    14641 ttttttataa ttaatttttt gcaatttgaa aaatgtatat tatatttgta aaaaacatta
    14701 tatttttatg aaaacaagta aatttttgtc aattttagta atagcatttt cattaattag
    14761 taatgcaaat gcacaaacaa acctagctgc ttggcatttt gatgtgttag cagccgctcc
    14821 taatactcct aaaattatcc aagctgatta tggacttcaa tcaaacagtg ctactattta
    14881 tttagacggt actcatggat catcagattg gaattcttct acaacaaacc cagaattaac
    14941 tgcttttgtt ggtagtacta caaatgatca acgtcctagt cctcttgctg gtcaggcatt
    15001 agcattagct aattcaagtg ctaataataa aggagttgta tttgctctat caactgaaaa
    15061 ttatgaaaat atcaaaattt cttatgctt
//
LOCUS       DAOPRN010000047        14361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_3583_length_14361_cov_103.530337, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000047 DAOPRN010000000
VERSION     DAOPRN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14361
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3583_length_14361_cov_103.530337"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..517)
                     /locus_tag="PKI65_06105"
     CDS             complement(299..517)
                     /locus_tag="PKI65_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HOB78049.1"
                     /translation="MLKHLFAIILITFVLFFSCSKDDCYDSKLEERHSGICPQNYDPV
                     CGCNGVTYSNDCVAASHGITHFTKGVCK"
     gene            622..1812
                     /gene="sucC"
                     /locus_tag="PKI65_06110"
     CDS             622..1812
                     /gene="sucC"
                     /locus_tag="PKI65_06110"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOB78050.1"
                     /translation="MNLHEYQSKNILEQYGIRVAKRFLACNADEAFDAAMKLKEITGS
                     DTCAIKAQVHAGGRGKAGGIKIAQSPKEAKELAAQIIGMRLITPQTNATGKLVKKVLV
                     EQNLYYVGPDNVQEFYISISCDRRNEKNVILYSPMGGVDIEKIAEDSPNMIFKEFIDP
                     VVGLMPFQTRKIAFNLGLSGDAFADMLDFITKLYRAYIDIDASLVEINPVLKTSDNLI
                     YPADAKIVLEDSALFRHPDLKKLQDIEEENELELQACEAGLNYVKLDGNVACMVNGAG
                     LAMATMDLIAIAGAKPANFLDIGGSASAEQIEKGFNIILKDPSVKAILINIFGGIVRC
                     DRVAQGILKACKNLNKIDLPIIARIQGTNANMAKEILENSNLKILTANSLDDVLECIK
                     KLNI"
     gene            1899..2633
                     /locus_tag="PKI65_06115"
     CDS             1899..2633
                     /locus_tag="PKI65_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOB78051.1"
                     /translation="MSMRDKYDNILIILLITSILLVLMYLLIGRSEYYFPKRDNFNVY
                     GIDLSYYQGDKIDWDSLLYGNDTPIEFVFLKASEGVNLQDIMFKEYAKELSKRNVCFG
                     AYHYFIAELSGNEQAKNFLNAIDGIDVKLPYVIDIEEYNDRELAIRNLNSFIHTINNE
                     TSHDIMIYTNISTYYDLIIGNELDHLLLWIASYKVDEPIIPGRYLFWQYSDRGRLKGI
                     SEKIDLNVFNGSYNDWMKYKKYCGCN"
     gene            complement(2844..3278)
                     /locus_tag="PKI65_06120"
     CDS             complement(2844..3278)
                     /locus_tag="PKI65_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78052.1"
                     /translation="MKKIFYLNLLLFLFISCNAQKIIEMEQSSEKLINANHMDIPDIK
                     PFFKIDTAWIEDDFLKIKISFIGNKNIPEFSLVWNGALMKSLPPKAVLVLLPKIDKLP
                     KGKKTVKMTLAYELSAIKETNYDEIILLLKDYNTQLRYTPKK"
     gene            complement(3356..4531)
                     /locus_tag="PKI65_06125"
     CDS             complement(3356..4531)
                     /locus_tag="PKI65_06125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB78053.1"
                     /translation="MLAIKIIFWISAILLIYNYLLYPLTLFIINHFTKNPKCLKPNVE
                     FSPNISIIIAAHNEGLVIADKLESIIDGDYPLDKLEILIGSDCSTDNTNVILSEWAEK
                     YPFIKPYFFTERQGKISILNKLIPKANNEILILTDANIIFSKKTISELVIYFSNPKIG
                     FVDSRIINIINSQDDISIPEQRYISLESKIKQYEGCIWGTAMGSFGGCYAIRKQLFVP
                     IPEDKLIMDDLYICMNILKSKYMGIYANDAIVYEKAVTLINTEYRRKIRIAAGAIQNL
                     FIFTPILLKFNILSFCFFSHKVLRWMGPFLLIIIILFSIILATTGHWLYILLASIFIL
                     SILISLIDRFILYPRNKTVKIFRFITHFYLANLATFIGWIRVMKGTKNSIWTPTPRA"
     gene            complement(4532..5740)
                     /locus_tag="PKI65_06130"
     CDS             complement(4532..5740)
                     /locus_tag="PKI65_06130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOB78054.1"
                     /translation="MRILIIANKFPYPPHDGGALATSTMIEGLVDAEQDIYLFTMTTP
                     KHPSDENNLPDHLIDKIIAETCFVDTNISTKDLVVNFLFKHTPYIAERFYSDAFNDRL
                     VKILENEKFDIIQLEGLYLLHLIPLIRQHSSAKVIYRAHNIEHYIWKRKAKNERNPFK
                     KIYFLNLYNRLKKYLKKYLNTYDGLVSISEPDYRIHKWLGQQKPEIVLPVGLVLSDYF
                     IYPISTDKIEQIAFIGGLDWLPNQEGLEWFITKVWHKILNIHPNAELHIAGRNCDDNL
                     QTQIKTFKNVIFYGEVPSSKEFLAAHSILIVPLFSGSGIRVKIIEGMAMGKAIVTTSV
                     GAEGIPIENKKHAFIADSIEDFADAINLLIKNPNLINQIGNNARELAKEHFSLEKLTD
                     DLLDFYKTLI"
     gene            complement(6244..6720)
                     /locus_tag="PKI65_06135"
     CDS             complement(6244..6720)
                     /locus_tag="PKI65_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78055.1"
                     /translation="MKTTLRPFIITILLFLSFIIYAQKDENYVVVNMNTKFILELETI
                     DSLHYQFKLKSQKPFNKKIKWDKPTKYLDGDLKNNQIQGVLGKGKFGDKINTILMIKN
                     GHDNTLSYKIKIKLPYKSEPISTSVIDLFPHVLATELWPYEIEYIIFLEFTKVDDY"
     gene            complement(6765..7337)
                     /locus_tag="PKI65_06140"
     CDS             complement(6765..7337)
                     /locus_tag="PKI65_06140"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOB78056.1"
                     /translation="MITLPPLPFEKNALEPYISATTLEFHYGKHHQAYVDNFNKLSAG
                     TEFENMPLEEVIKKATGGIFNNGAQVWNHTFYWNCLNKPDTTKPSDKLLKAIENQFGS
                     LDNFKEQFSNAAVTLFGSGWAWLCKDEFGKLYINQTSNAENPIKNGLTPIMTIDVWEH
                     AYYLDKQNRRPDYVKDFWHILNWDSVNQLY"
     gene            complement(7522..8364)
                     /locus_tag="PKI65_06145"
     CDS             complement(7522..8364)
                     /locus_tag="PKI65_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78057.1"
                     /translation="MFKSKYFHFLLIIFLINNQIFSQDNLCVSYTIYHNNKPFKNIIF
                     NNNNKPIIVNDLSLNIKEIYLYRDTLLIKSQIIFNNDTISYEYFYNEKNKLEKIRSFN
                     NANLYSVTFFNYYDSTFYEKIEYTNTQRYITRYYYDILGRLRELSYIELTDFSTNFSQ
                     RFVYDYDENGNICRKIQSSEYDTLCIWLYEYASDNILIKEVIKNKIDNNNALEKYYIY
                     NNKDKLKQVLYIKDSFIFKKETYNYSLIKENLKNKKVIDYSTRPIKKEKCSYKIKKTP
                     CNTD"
     gene            8476..9795
                     /gene="miaB"
                     /locus_tag="PKI65_06150"
     CDS             8476..9795
                     /gene="miaB"
                     /locus_tag="PKI65_06150"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOB78058.1"
                     /translation="MMKTFYIETYGCQMNVVDSEIVASILIRNGLIQTYNPQDADIIL
                     LNTCSIRENAEMRIKSRIKQLGSFKKTNPNLRLGIIGCMAENLKEKLFVELPQLDLLA
                     GPDVYRLLPEILKQINDENKIINTTLSKEETYADVLPFRYDSNGISAYVSIMRGCDNF
                     CSYCVVPYTRGRERSRDASSIINEVLSLSENGFKEITLLGQNVNSYHYRDIDFPALIS
                     LLAKNFPNLLFRFTTSHPKDLSIELIEAMASFPNIAKSIHLPVQSGSNEVLKRMNRKY
                     TRDDYLEKVYLLRRLIPDITISTDIICGFCGETEEDHLQTIDLMKEVNYFFAFMFKYN
                     ERPNTLAAKKYADDVTDDIKSRRLKEIIDLQQKISYMLNKQDIGKIFKVLVEGISKKN
                     EKEIFGRNSQNKVIVFAGDLTQKGQFVDVEVTNCTSATLIGRQNFDK"
     gene            9878..10663
                     /locus_tag="PKI65_06155"
     CDS             9878..10663
                     /locus_tag="PKI65_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOB78059.1"
                     /translation="MNVLDKEKNHIIFLGTGTSTGIPVISCKCATCMSKDIRDKRFRT
                     SAFINYNGLRLVIDCGPDFRLQMINNKIDDIDAILITHGHRDHIAGIDDIRPFNFILG
                     KVIHLYAKDEIMDQLDKEFPYILNPNGYFGSPQISYHPISNDVFYICDQPIIPIPIYH
                     GNLEILGFRFNNLAYITDASMIPESSIQLIKGVDILVINALRRSPHISHLSLPQALEI
                     IKQVNPNKAYLTHLSHFLPPYKQILKDLPPNVIPAYDGLKVTF"
     gene            11129..12475
                     /locus_tag="PKI65_06160"
     CDS             11129..12475
                     /locus_tag="PKI65_06160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB78060.1"
                     /translation="MEFKDALKNQTLSREDTINITKKYIEINRYLFAQKTIYNYLKTN
                     KFEEELVEILADIYNFNGEYKKAKKLYSTIYKKSKNDDFKHHIIQKIKKTEERIELSK
                     NQIKLAVVVNEDEDDFLDPIIKGINYDYWVKKYIIPRNYARFLNFLSRALKNKIINKK
                     LYKFILKITHKKLNKALKWADIIWVEWATDNAIAASLLKPNSKKLFIRLHRYEAFDFY
                     PLLIEWQRVDNLVIVNNYITNIFNERNVNIDKRKFILIPNGVDLNRYGYCQHLQGFNI
                     AWISHITPMKNLHLALEILRILLEIDDRYILHVAGNFTDLVYKKYIKQSIKNMNLVKN
                     VIFYDWVNDINEWLEDKNYLLSTSVCEGHPFNVIEAMAKGIKPVINNFCGAELVFDKN
                     FLFNNIDEAIKMITRDQYDSQLYRNYIIEKQWTEKDQVDKFKRLIEELNKNINISL"
     gene            complement(12459..13478)
                     /gene="namA"
                     /locus_tag="PKI65_06165"
     CDS             complement(12459..13478)
                     /gene="namA"
                     /locus_tag="PKI65_06165"
                     /EC_number="1.6.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH dehydrogenase NamA"
                     /protein_id="HOB78061.1"
                     /translation="MSKLFTPFRLKNLELKNRIAMSPMCMYSAGDDGQITDWHQIHYA
                     TRAIGGIDLILQEATAVEKRGRITANDLGIWDDTFVADMKKFVKLIHSLNCKIAIQLA
                     HAGRKCEVKNEKIIAPSAIKWNENYPIPTEMNKKDIRDVIISFTQAATRAVLAGYDAI
                     EIHAAHGYLIHEFLSPLSNHRNDEYGGNTINRTRLLQEILTEIKKIIPTDMPIFIRVS
                     ATDYVKGGLNIDETMTIIDIIKDLVDIVDCSTGGLLSPRIDLYPGYQIKFAEKIKNSL
                     NIPTAAIGLITRPEMAEEIVGNERADLVLLGRVLLRQPYWPMYAAHILKADFIYPTQY
                     IRGKY"
     gene            13604..13840
                     /locus_tag="PKI65_06170"
     CDS             13604..13840
                     /locus_tag="PKI65_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78062.1"
                     /translation="MNNNQNKLDIKALETWLWDAARKLHGPIDVLSKYRLGPDDVKPN
                     TLFIEDAVVLLQEAEEERQKADEKMKAISEELGV"
     gene            13976..>14361
                     /locus_tag="PKI65_06175"
     CDS             13976..>14361
                     /locus_tag="PKI65_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOB78063.1"
                     /translation="MKATEANLLEFIKKSNQFSIPIYQRLYSWTEKECGRLWNDIMTV
                     GSKNIPSHFIGSIMYIEKGLYQISKQEPLLVIDGQQRLTTISLLLEALARKIGDNEVE
                     GFSARKIRNYYLINPLETGDRKYKLLL"
BASE COUNT         5217 a         1814 c         2073 g         5257 t
ORIGIN      
        1 aatttaataa attttctgat attaatcctg tataaatttt aacagactaa atatttcatt
       61 ctccccttag agatttattg aaaaaagaaa aataaatttt ttgaattagg cgtttaagaa
      121 ataattggct tttctttgtg ttctctgtgg tttcattgtg ttctctgtgg ttaatattct
      181 atatgtaact tcttattata ttttttaacc caaaggccac aaaatttcat atacaaagaa
      241 cgcagagaga gctattttgg ggttattatc taaatgtcta attcaataat tttttaattt
      301 atttacatac tcctttagtg aaatgagtaa taccatgact agcagcaacg cagtcattag
      361 aatatgtgac tccattacaa ccgcaaacag gatcataatt ttgtggacaa ataccggaat
      421 gtctttcttc taatttgctg tcataacaat catctttact acaactaaaa aataatacaa
      481 atgttattaa aataattgca aataaatgtt ttagcataac aatttattta atataacgat
      541 atcaatctat taattattat aatttttatt ttctaatttc ttattaattt tacctacttt
      601 taattattaa aaataaataa tatgaacctc catgagtatc aatcaaaaaa catattagaa
      661 caatatggca ttagagttgc gaaaagattt ttggcttgta atgctgatga agcttttgat
      721 gctgcaatga agttaaaaga gataactgga tcagatacct gtgctataaa agctcaagta
      781 catgctggtg gtagaggtaa agctggtggc ataaaaatag ctcaatcacc taaagaagct
      841 aaagaacttg cagctcaaat cattggtatg cgactgataa cacctcagac caatgcaaca
      901 ggtaaattag ttaaaaaggt tctagtagag caaaatcttt attatgttgg tcctgataat
      961 gtgcaagaat tttatataag catatcttgt gatcgtagaa atgaaaaaaa tgtcatactt
     1021 tattctccta tgggcggtgt agatatagaa aaaatagcag aagatagtcc aaatatgatt
     1081 tttaaagaat ttattgatcc tgttgtgggt ttaatgcctt ttcaaactcg taaaattgct
     1141 tttaatttag gattgagtgg agatgctttc gctgatatgc tagattttat taccaagttg
     1201 tatcgagcat atatagatat tgatgcttca ttagtagaga taaatcctgt tttaaaaaca
     1261 agtgacaatc tgatatatcc tgctgatgcc aagatagtat tagaggattc agcgctgttt
     1321 aggcatcctg atttaaaaaa attgcaagat atagaagaag aaaatgaact agaactacaa
     1381 gcatgtgagg ctggattaaa ttatgtaaaa ttagatggca atgttgcttg tatggttaat
     1441 ggtgctgggc tagctatggc cactatggat ttaatagcta tagcaggagc caaaccagca
     1501 aattttcttg acataggcgg atcagctagt gctgaacaaa tagaaaaagg ttttaatatt
     1561 attttgaaag atccctctgt taaagcaatt ttaataaata tctttggtgg tatagtcaga
     1621 tgtgatagag tagctcaagg aatattaaaa gcatgcaaaa atcttaacaa aatagatcta
     1681 cctattatag cacgtataca aggtactaat gctaatatgg caaaagagat tttagaaaat
     1741 tctaatctta aaatcttaac tgcaaattcg ctagatgatg tattagaatg tattaaaaaa
     1801 cttaacattt agataaatta tcattttatt cctttacatt cttagcattt ataaaatata
     1861 ggaatgattt ttgtttattt ttaatagatt ttaattgtat gagcatgaga gataaatacg
     1921 ataatatttt gattattttg ctgattacta gcattttact tgtattaatg tatttattga
     1981 taggtagatc tgagtattat tttcctaaaa gagacaattt caatgtttat ggtatagatt
     2041 tatcttatta tcaaggggat aaaatagatt gggattcact cttgtatggt aatgatacac
     2101 cgattgaatt tgttttttta aaagcttctg aaggtgttaa tttacaagat attatgttta
     2161 aagagtatgc aaaagaatta tctaaaagaa atgtttgctt tggggcttat cattatttca
     2221 ttgctgaatt aagtggaaat gagcaagcta aaaatttttt gaatgctata gatggtattg
     2281 atgtaaaact accttatgtt attgatattg aagaatataa tgatagagag cttgcaatca
     2341 gaaatttaaa tagttttatt cataccatta ataatgaaac ctctcatgat ataatgattt
     2401 atactaatat ttctacttat tatgatttaa taattggtaa tgaactagat catttactat
     2461 tgtggatagc atcttataaa gtagatgaac ctataattcc tggtaggtat ttattttggc
     2521 aatattctga tcgtggacga ttaaaaggta tatcagaaaa aatagatttg aatgttttta
     2581 atggtagtta taatgactgg atgaaatata aaaaatattg tggttgtaat taattgatag
     2641 ccaaactaat taatatatct aaatatgtat taggtaaatg aatatttagt catattgaaa
     2701 agtataataa aaatctgggt aaaatatata atcttcatat tagtaatttt tattaaaatt
     2761 taaaatttgt aatgagcttg ccaaattatt tagaattctg aatttttgta taatatattc
     2821 ttgattatta atttacaaat tttctatttc ttgggcgtat atcttaattg agtattataa
     2881 tctttgagca aaagtattat ctcatcatag ttggtttctt taatagcact gagttcataa
     2941 gccaaagtca ttttaacggt ttttttacct tttggcagtt tatcaatttt aggtagtaat
     3001 actaaaactg cctttggtgg caaggatttc atcaaagccc cattccatac taaagaaaat
     3061 tctggtatat ttttattgcc aataaaagaa attttaattt ttaaaaagtc atcctctatc
     3121 caagcagtat ctattttaaa aaaaggctta atatcaggta tatccatgtg attagcattt
     3181 attaactttt cactactttg ctccatttct attatttttt gcgcattaca agatatgaat
     3241 aaaaacaaca aaagatttag ataaaaaatt tttttcattt tcttattttt tattcaaaat
     3301 tataatattt attttaatta gatataaata tttattttaa ttttattaaa aattgtcaag
     3361 ctctaggagt tggtgtccag atactattct tagttccttt cattactcta atccagccta
     3421 taaaagtagc taaattagct aagtaaaaat gtgtaataaa tctaaatatt ttaacagttt
     3481 tattacgtgg gtataatata aatctgtcaa ttaaacttat caaaatacta agaataaata
     3541 tgctagcaag taaaatatat aaccaatgac ctgtggtagc caaaataatt gagaataaaa
     3601 taatgataat caataaaaat ggccccatcc agcgtaagac tttatgagag aaaaaacaaa
     3661 aactcaaaat attaaattta agtaagatag gagtgaagat aaataaattt tgtatagcac
     3721 cagctgcgat acgtattttt cgcctatatt ctgtgtttat caatgtaact gctttttcgt
     3781 aaactatagc atcatttgca tatattccca tatatttact ttttaaaata ttcatacaaa
     3841 tgtacaaatc atccattatt aatttatctt ctggtatagg tacaaaaagt tgttttctta
     3901 tagcatagca tcctccaaat gagcccattg ctgttcccca gatacaacct tcatattgct
     3961 tgattttgga ttccaatgaa atgtatcttt gctctggtat tgatatatca tcttggctgt
     4021 tgataatatt tattattctg ctgtctacga agccaatttt aggattgcta aagtaaatta
     4081 caagttctga aatagttttt ttgctaaata taatattagc atctgtcagt attagaattt
     4141 cattattagc ttttggaatt agcttgttca aaattgaaat tttaccttgt cgctctgtga
     4201 aaaaatatgg cttgataaaa ggatatttct cggcccattc agataaaatt acattagtat
     4261 tatcagtaga gcaatcagaa cctatgagta tttctaattt atctaaagga taatctccat
     4321 ctataataga ttctaattta tctgctatta ctagcccttc attatgtgca gctataataa
     4381 tagatatatt aggtgagaat tctacgtttg gcttaagaca tttaggattt ttagtaaaat
     4441 gattaataat aaataatgta agtggataaa gtaaataatt ataaatcaat aaaatagcac
     4501 taatccaaaa aattattttg attgctaaca tttatatcaa agttttataa aaatctaata
     4561 aatcatctgt taatttttct aaagaaaagt gctcttttgc taattcgcga gcattatttc
     4621 caatttgatt tatcaaattg ggatttttta tcaaaagatt tattgcatct gcgaagtctt
     4681 caatagagtc tgctatgaaa gcatgttttt tattttctat cggtatacct tctgcaccga
     4741 cactagtagt tacaattgcc tttcccatag ccataccttc tattatttta actctgatac
     4801 cactaccaga aaagagtggc actatcaata tgctatgtgc agctaagaat tctttagatg
     4861 aaggaacttc accataaaaa ataacatttt taaaagtttt gatttgagtt tgcaaattat
     4921 catcacagtt tctgccagct atgtgcaact cagcattagg atgaatattt aatattttgt
     4981 gccatacttt tgtgataaac cattctaaac cttcttgatt aggcagccaa tctagtccac
     5041 cgataaaagc tatttgctcg atcttatctg tagaaatagg atatataaaa taatctgata
     5101 aaactaagcc aacaggcaaa actatttctg gcttttgctg gccaagccat ttatgtattc
     5161 tataatcagg ttctgaaata gaaacaaggc catcgtaggt atttagatat ttttttaagt
     5221 actttttgag cctattgtat aagtttagaa aataaatttt tttaaaagga ttgcgttcgt
     5281 tttttgcttt tcgtttccaa atataatgct ctatgttatg tgctctataa attactttag
     5341 ccgatgaatg ctgacgtatg agaggtatta gatgtaatag gtataatcct tctaattgta
     5401 tgatgtcgaa tttttcattt tctaatattt ttactaatct atcattaaat gcatctgaat
     5461 aaaaacgttc agctatgtaa ggagtgtgct taaaaagaaa attgacaaca agatctttag
     5521 tagagatatt agtatctacg aagcaagttt cagctattat tttatctatc agatgatcag
     5581 gtagattatt ttcatctgaa ggatgcttag gtgtagtcat agtgaataaa taaatatctt
     5641 gctcggcatc tacgagcccc tctatcatag tagaggtagc caaagctccg ccatcgtgtg
     5701 ggggataagg aaatttatta gctattatga gaatacgcat aaaaaaaatt atttatcaaa
     5761 gataataaga agttttgaaa ttgaactaaa tgataaattc tgagtttttt tttaatgaaa
     5821 tatatcgaat ttaataatta aaggacccta agaaaaatta gtaatcatag cactcatgtg
     5881 atttttaaaa tgatttgtta atatttttaa tgaaatgttt aaatctatta atgttattat
     5941 ataaacttat aattatatat aaattcacat gtatatttaa gttaactata atttattata
     6001 atatttattg atttttattg aatttatgat aatactaata aaaatcaata aatagagtat
     6061 tatatgtttt taaatataaa cttgataaat tttaacatta ttaaaattgc attaaaatca
     6121 taccaatgcg cttactaaat aaattcttaa aaagataaat ttttgtttaa ttttcgtctt
     6181 tacctgagac accaaaaaaa taataataaa tgagagttta aaaattttta aacccataca
     6241 tatttaataa tcatcgactt tagtaaattc taaaaatata atatactcaa tttcataagg
     6301 ccatagttct gtggctagta catgagggaa aagatcaata actgatgtac taataggttc
     6361 agatttgtat ggcaatttta ttttaatttt ataactcagt gtattgtcgt gtccgttttt
     6421 aatcatcaga atagtattga ttttatcacc aaattttcct tttcctaaaa caccttggat
     6481 ttgattattt ttcaaatcac catctagata tttagtaggc ttatcccatt tgattttttt
     6541 attaaaaggt ttttgtgact ttagtttaaa ttgatagtgc aaagagtcta tagtttctaa
     6601 ctctaaaatg aatttggtgt tcatgttaac tactacataa ttttcatctt tttgggcata
     6661 aattataaat gataaaaata ataatatcgt aataataaaa ggtctaagag ttgttttcac
     6721 gattttatat tattgcaagt atgattttct ttttatataa agtttcagta taactgattt
     6781 acagaatccc aatttagaat atgccagaaa tcttttacat agtctgggcg tctattttgt
     6841 ttatctaaat aataagcatg ctcccacaca tctattgtca taattggcgt taatccattt
     6901 tttataggat tttctgcatt agatgtttga ttgatgtata gcttgccaaa ttcatctttg
     6961 caaagccatg cccatccaga gccgaaaagt gtaactgcag catttgagaa ctgttccttg
     7021 aaattatcta aactaccgaa ttgattttct atagctttta gtaatttatc agaaggttta
     7081 gtagtgtctg gtttatttaa acaattccaa tagaatgtgt gattccatac ttgtgctcca
     7141 ttattaaaaa ttccaccagt agcttttttg ataacttctt ctaatggcat gttttcgaat
     7201 tcagtgccag ccgataattt gttaaaatta tctacatatg cttgatgatg tttaccataa
     7261 tgaaattcta atgtagtagc actgatataa ggctctagtg catttttttc gaatggtaaa
     7321 ggaggtaatg taatcataac ttttattttt tatgtttatg caaatttaaa gaaaaaaatc
     7381 tataattatt caataattta tttattaaat gatatttatc atatattttt aactgtcaat
     7441 tacaactttc taaagctgtt taacattaaa attaatttgt gtaattaaaa aaaacaaacc
     7501 taatatcata aaatcaaatc actaatcagt attacaaggt gtttttttta ttttatatga
     7561 acatttttct tttttgatag gtctggtaga ataatcaata acttttttgt tttttaaatt
     7621 ttctttaatc aatgaataat tgtaagtttc ctttttaaag ataaaagaat cttttatgta
     7681 taatacttgc ttgagtttat ctttattatt atatatataa tatttttcta gagcattatt
     7741 attatcaatt ttatttttta taacttcttt gattaatata ttatctgatg catactcgta
     7801 tagccatata catagagtat catactcact agattgtatt tttcgacata tattaccatt
     7861 ttcgtcataa tcatatacaa atctttgtga aaaattagta gaaaaatctg taagttctat
     7921 atatgatagc tcgcgcaacc tacctaatat gtcataataa tatcgagtaa tataacgttg
     7981 tgtattagtg tattctattt tttcataaaa tgtactgtca taatagttaa aaaaagtaac
     8041 tgaatagaga ttagcattat tgaaagatct gattttttct aatttatttt tttcattata
     8101 aaaatattca taactaatag tatcattatt aaaaattatt tgacttttaa taagtaaagt
     8161 atcacgatat aaataaattt cttttatatt aagggaaaga tcatttacaa tgataggttt
     8221 attattatta ttaaaaatta tatttttaaa aggcttatta ttatgataaa tagtataaga
     8281 gacacataga ttatcttgcg aaaatatttg attattaatt aaaaaaatta ttaataaaaa
     8341 atggaaatat ttacttttaa acatagttta acaaaattat aaaaagttta attaattagt
     8401 tatattagta ataaatttta caattcattt tttaaatgta aatttgtatt tactttttat
     8461 atatgtcaaa aatgtatgat gaagactttt tacatagaaa cttatggttg ccagatgaat
     8521 gtcgtagata gcgaaatagt agcatctatt ttgattagaa atggtttaat acaaacatac
     8581 aacccccaag atgccgatat catattacta aatacctgta gtattagaga gaatgcagaa
     8641 atgaggataa aatctagaat caaacaattg ggttctttta aaaaaactaa tccaaatctt
     8701 agattaggta ttataggttg tatggctgag aacttaaaag aaaaactatt tgtagaatta
     8761 cctcaattag atttattagc tggtcctgat gtatatagac tactgcctga aatattaaaa
     8821 cagataaatg atgaaaataa aatcataaac acaacactat caaaagaaga gacttatgca
     8881 gatgttttac catttcgtta tgatagtaat ggtatatctg cttatgtttc tattatgcgt
     8941 ggatgtgata atttttgtag ctattgtgta gtgccctata ctcgtggaag agaaagaagt
     9001 agagatgcca gctccattat caatgaagtt ttatcattat cagaaaatgg ttttaaagaa
     9061 attactttgc taggtcaaaa tgtcaattct tatcattatc gtgatattga ttttcccgcc
     9121 cttatatctt tattagcaaa aaattttcca aatttacttt ttcgatttac tacctcgcat
     9181 cctaaagatc taagtataga attgatagaa gctatggctt cctttccaaa cattgctaag
     9241 tctatacatt tacctgtaca atcaggatct aatgaggtat tgaagcgaat gaatagaaaa
     9301 tatactaggg atgattattt agaaaaggtc tatttacttc gtcgacttat acccgacatt
     9361 actataagta ccgatattat ttgtggtttt tgtggtgaaa cagaagaaga tcacttacag
     9421 acaattgatc taatgaaaga agtaaattat ttttttgctt ttatgtttaa atataatgaa
     9481 cgtccaaata ctctagctgc taaaaaatat gctgatgacg tgactgatga tattaagtca
     9541 cgaagactaa aggaaataat tgatttacaa caaaaaatct cctatatgct taataaacaa
     9601 gatataggta aaatttttaa agtattagta gaaggtatat caaaaaagaa cgaaaaggaa
     9661 atttttggtc gtaattccca aaataaagtt atagtatttg caggtgatct aactcaaaaa
     9721 ggacaatttg ttgatgtaga agtaactaat tgcacttctg ctactctaat aggaaggcaa
     9781 aattttgata aataaagatt tcattatgaa tttttttagt tatataaaat taaaatatgt
     9841 aaatttaatt tataaaaaat atagataaat agtaactatg aacgtcttag acaaagaaaa
     9901 aaatcatatt atttttttag gtactggtac ctctacaggg atacctgtta ttagttgtaa
     9961 atgtgctact tgcatgtcaa aggatatacg tgacaaaaga tttcgtacct ccgcttttat
    10021 taattataat ggattgagat tagtgataga ttgtggacca gatttcagat tgcaaatgat
    10081 aaataataag atagatgata tagatgccat acttatcact cacggacata gagatcatat
    10141 tgctggcata gacgatatca gaccttttaa ttttattctt ggtaaagtaa ttcatcttta
    10201 tgctaaagac gagataatgg atcaattaga caaggaattt ccatatatac ttaacccaaa
    10261 cggatatttt ggctcgccac agataagcta ccatcctata agtaatgatg ttttttatat
    10321 ttgtgatcaa ccaataatcc ctataccaat ataccacggc aatttagaaa ttttaggatt
    10381 tagatttaat aatttagcat atattactga tgcaagcatg ataccagaga gtagcattca
    10441 attgataaaa ggagtagata tattagtaat aaatgctctg cgtagaagcc cccatatctc
    10501 acatttatcc ctgcctcaag ctttagaaat aatcaagcaa gtaaatccta ataaagcata
    10561 tctaacacat ttatcacatt ttttacctcc atataaacaa attttaaaag atttaccacc
    10621 taatgtaata ccagcatatg atggattgaa agtgactttt tgataactaa aaattgatat
    10681 tataactttt ttatgtagct ctaggagtta aacgagaaaa ctcgattttc atatatttat
    10741 caatatctat acaggatcaa tttttgaata attattaaat tgacaaaaaa caaaagtttt
    10801 acaagcaatt gtagagaact tagatttttt tattttcatc taatgattaa ttttcaagat
    10861 tagtcctttt ctgtagtgat tactagactt catttttttg caattattat tattttttga
    10921 ttaacataat ttatttgatg atttattgaa atgtaattta tatttttaat aaatatacct
    10981 acatcagctt ttatctgaga tataatgagt aaaaaataaa taatagtcgc tgtatgagca
    11041 attcacaaat tgttttttat aatatcattt tttttaaaaa tttattaatt ttactgaatt
    11101 attctaaaaa atactataat taataattat ggaatttaaa gatgcattaa aaaatcaaac
    11161 attatctagg gaagatacta ttaatatcac taaaaaatat attgaaatta atagatattt
    11221 atttgcacaa aaaactatat ataattatct aaaaacaaat aaatttgaag aggaattagt
    11281 tgaaatttta gcagatattt ataactttaa tggtgaatat aaaaaagcta aaaaattgta
    11341 ttctactatt tataaaaaat caaaaaatga tgactttaaa catcatatta ttcaaaagat
    11401 aaaaaagaca gaagaaagga ttgaactctc taaaaatcaa atcaaactcg ctgtagttgt
    11461 taatgaagat gaggatgatt ttttagatcc tattattaaa gggataaact atgattattg
    11521 ggtaaaaaaa tatataatac caagaaatta tgcacgattc ctaaattttt tatcaagagc
    11581 tctgaaaaat aaaattatta ataaaaaatt atataaattt attttaaaaa ttacacataa
    11641 aaaattaaat aaagctctga aatgggcaga tataatttgg gtagaatggg ctacagataa
    11701 tgcaatagct gctagtttgc taaaaccaaa ttctaaaaaa ttatttataa ggcttcatcg
    11761 atacgaagct tttgattttt atcctttatt gatagaatgg caaagagtag ataatttagt
    11821 tatagtaaat aattatatca caaatatttt taacgaaaga aatgttaata ttgataaaag
    11881 gaaatttata ttgattccta atggcgttga tttaaataga tatggttact gccagcattt
    11941 gcaaggtttt aatatagctt ggatatcgca tattactcct atgaaaaacc tacacttagc
    12001 attagaaata cttagaatat tattagaaat cgatgataga tatattctgc atgtagctgg
    12061 caattttact gatcttgttt ataaaaaata cattaagcaa tctattaaaa atatgaattt
    12121 agtaaagaat gtaatatttt atgattgggt aaacgatata aatgaatggt tagaggataa
    12181 aaattattta ttatcgacta gtgtatgtga ggggcatcca tttaatgtta tagaagccat
    12241 ggctaaaggc atcaagccag tgattaataa tttctgtgga gcagagttgg tttttgataa
    12301 aaatttttta ttcaataata ttgatgaagc tattaaaatg ataactagag atcagtacga
    12361 ttcacaatta tatcgtaatt atattatcga aaaacaatgg acagaaaaag atcaagtaga
    12421 taaattcaaa agattaattg aagaattgaa taaaaatatt aatatttccc tctgatatat
    12481 tgagtaggat aaatgaaatc ggcttttaga atatgtgcag catacatagg ccaatatggt
    12541 tggcgtagaa gtactcgccc tagcaaaact aaatcagccc tttcattacc tacaatttct
    12601 tcagccattt ctggtcttgt tattaagcct attgctgctg taggaatatt gagtgaattt
    12661 tttatttttt ctgcaaattt tatttgatag ccaggatata gatctatcct aggagataat
    12721 aatccaccag tgctgcaatc tacgatatct actagatctt ttattatatc tataattgtc
    12781 attgtctcat ctatatttag tccacctttt acgtaatcgg ttgcggagac tctaataaaa
    12841 atgggcatat cagtgggaat gattttttta atttctgtta agatttcttg taataatcta
    12901 gttctattaa ttgtattacc tccatattca tcatttctat ggttagataa tggtgataaa
    12961 aactcatgta ttaaataacc atgtgctgca tggatttcta tagcatcata accagctaat
    13021 acggctctag tagcagcttg agtaaaagat ataattacat ctctaatatc ttttttgttc
    13081 atttctgtag gtataggata gttttcgttc catttgatag cagagggggc aatgattttt
    13141 tcatttttta cttcgcattt cctaccagca tgagctagtt gtattgcgat tttacaattc
    13201 aatgaatgaa tgagcttaac aaattttttc atatcagcta caaatgtatc atcccaaata
    13261 cccaaatcat tagctgtgat gcgacctcgt ttttctactg ctgtagcttc ttgaagtatc
    13321 aagtctatgc ctcctatagc tcgtgtagca taatgtatct gatgccaatc agttatttgc
    13381 ccatcatctc cagcactata catacacatt ggcgacatag ctatcctatt ttttaattct
    13441 aaatttttta atctaaaagg tgtaaataat ttgctcatag tagattgatt gtttttttta
    13501 ttattacaaa attaataaaa tagatggtat tattgttttt tatataaaaa aagattttat
    13561 ctttttgcta aataatatca agatttattt aaacaacaaa attatgaata acaatcaaaa
    13621 taaattagat ataaaagcgt tagaaacgtg gctctgggat gctgcacgta aactgcatgg
    13681 ccctatagat gtgcttagca aatataggct cggtcctgat gatgtgaaac ctaacacttt
    13741 gtttattgaa gatgctgtag tgctactgca ggaagctgaa gaagaaagac aaaaagcaga
    13801 tgaaaaaatg aaagcaatct cagaagagtt gggagtatag gaataacaca gaaaacgaag
    13861 aaatcatcaa ccgcatatgc cctgctaaag tcagagtggg aggtagtgaa gtgattttta
    13921 aaaatataaa ttttttcata attttgaaaa atttaaatta ataaaagttt aacttatgaa
    13981 agcaactgaa gctaatttat tagaatttat aaaaaaatct aaccagtttt caatccctat
    14041 ttaccagaga ctttatagct ggacggaaaa agaatgtgga cgattatgga atgatattat
    14101 gactgtaggg agtaaaaata ttcccagtca ctttataggc tctatcatgt atatagaaaa
    14161 aggtctatac caaatatcga aacaggaacc gttacttgtc attgatggtc agcaacgttt
    14221 aaccacaatt agtttattgc tcgaggcatt ggcgcgtaaa attggtgata acgaagtaga
    14281 aggattttca gcaagaaaaa ttagaaatta ttatcttatc aatcctctcg aaaccgggga
    14341 tcgaaaatat aaattacttc t
//
LOCUS       DAOPRN010000048        14062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_3668_length_14062_cov_105.436353, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000048 DAOPRN010000000
VERSION     DAOPRN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14062
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3668_length_14062_cov_105.436353"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..1421)
                     /locus_tag="PKI65_06180"
     CDS             complement(360..1421)
                     /locus_tag="PKI65_06180"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOB78064.1"
                     /translation="MKKYKFISVLTIFILVPLFMFAGNEDRVGEAGASELLINPFPRS
                     AGWGLANMSQVSGFEAVYLNPAGIAFTNKIDVAFANTQWLKGSGVTVNAFGFSAKIGK
                     QKNSVLALGVMTMSFGDIMITTVDKPEGGIGYYTPNFLNVTASFAHSFSSSIHVGASV
                     KIVNEAIADLNATGVAIDAGIQYIPSSNENIRLGIALRNIGPALRFSGDGFSFRGNVF
                     GSDMTMTLEHRSEKFELPTQLLISAGYIFHLQEDVEDHLLSIGGSFISNAFSRDQFAL
                     GLEYNFKNIFMVRGSYCYEKGITSTDDRATAYTGPAFGATVQAPLNKEKSSFLGFEYS
                     YRISDPFQGTHQIGVKLML"
     gene            complement(1440..5495)
                     /locus_tag="PKI65_06185"
     CDS             complement(1440..5495)
                     /locus_tag="PKI65_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78065.1"
                     /translation="MIKKVFVSIVLALFVGLVVGENVPTAGINKRTMSSDELKEAAAG
                     CLAGAFYTELNINNVRTRINSGGDMWWDLQTAGGRGTAQYEIPKGSKKTSMFAGALWI
                     GGIDVNGQLKLAAQRYRQIGVDFFPGPLSTDGAAFIDADECTRWDKIWTIYRKDVDQF
                     LAWWTEDSANISSYPIPKYFFDYPAHGDPTKGQSFYIAPFYDHNGDGTYNPYDGDYPY
                     YDIDNSLCKQELPTLETEMGYVFGGIMADQVLKGDQTLFWVFNDRGNIHTETQGQQIG
                     FEIRAQAFAFTTNDEINNMTFYSFEIINRSTYTLYETYMSLWTDPDLGYAWDDYLGCD
                     VERGLGYCYNGKAVDGTGQIEAYGDQPPAIGIDFFQGPYMDPDGIDNPKYNIYTDADG
                     NTIYEQICDESINGVNFGDGIVDNERFGMRRFVYHNNTGVPNYMTDPDIAIEYYNFLK
                     GIWKDNTKMMYGGNAHISSGAYGPECDFMFPGDTDPCDWGTGGLPPNGPRYWTEETAG
                     NLPHDRRFMQSAGPFTLRPGALNYVTVGIPWARATSGGPFASVELLRKVDDKCQRLFE
                     NCFKVLDGPDAPDLVIQELNRELILFITNKPTSNNANEDYAEVDPSIVSPIGTPPDQK
                     YDSVYRFQGYQIFQLKDATVGPAELHDAAKARLVAQCDVKDSVSRLINFYYSEDLDAM
                     MPVEEVNGQNEGIKHSFRIVEDQFASGDKRLVNNKKYYYMVVAYAYNEYKKYSHIPGS
                     AGSIDGQAKPYLMGRKNIQVYTAIPHITEPENSGTVIRANYGDGVQITRIEGQGNGGN
                     ILDILPESEKEILANNFVQELKYKTGRGPINVSVVDPLNVIDARYILKFNKNVSQSNL
                     IKGNCDWEVEVYSPDWSTLIGKFNSQKAISNRNEQLFLDLGISITIEQCLYPGDENDP
                     TNGFLEATMEYKDSTRRWLSGVPDEEGSSSLNWIRSGTLHNVDDQSLNDYDPSGWLDP
                     KQYYEKVLNGTWAPYRLVSSGANHPIASGMASYISLNKLSNLASVDLVLTPDKDKWTR
                     CVVIETCDDINLAQGGAAKMAPRKATSVDKNGNPDPNYDKPTGMGWFPGYAINVETGE
                     RLNIIFGEDSWLAGENGRDMIFNPTHRIMTNLGELLLGGKHFVYIIGHIGDNDFQDNY
                     PPAYDEGQWIYNKLNSTSLTDRRKVWNSALWVGIPLSVENENWLDNEVRLRFRVKRPY
                     DLYYSTPASANTNPQNDNRPMYSFSTENLTTIKGDLQTAKDALDLINVVPNPYYAWNA
                     YEINQVDNRVRITNLPEKCTVSIYSTNGQLIRRYTKDDPEITFVDWDLKNHAGIPIAG
                     GLYIIHVNAPGIGEKVVKWFGALRPIDLNAF"
     gene            complement(5515..9201)
                     /locus_tag="PKI65_06190"
     CDS             complement(5515..9201)
                     /locus_tag="PKI65_06190"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOB78066.1"
                     /translation="MRARLLSLVFLMLISFLALAQSSGTIMGTITEEGTNKPVPFAHV
                     AIMSGERIVTGGVTDIDGRYKIAPVPPGTYNIRISAVSYNTKVIEGVVISPDKITYIN
                     EKISLKTEVLDVVEVVGFRDKPIDKGNVSTATTMTATEISKLPTRDAIGVAQTAAGVF
                     KQDDGSTDVNIRGQRESGTVIYIDGIRTIGTTNLPKSALDQVTVITGGTPAEYGEATG
                     GVINITTKSGSTKLSGGIELVTSQFLDPYGYNLLGFSLEGPLFKSKKDPKRSIFNFFL
                     AGELSYEKDPRPFAKGLYKAKDDVVEYLEKNPLRPSQYGDFGAHPNAAFIRMKDLEHI
                     KAKLNADAKQVNLSAKITVNTGPNIQLTFGGSFRYANSMGYVHSFSLFNWKNNPQILT
                     YEGRAYVRFSQDFTRRSLESKDTTKKLISNIYYSLQADYSKINQVIQDPDFKDDLFKY
                     GYVGKFTTTKARSYAFMEYYPLLDLNNVWVQDNFYDTHVDFEPGTANPALAAYTSRFY
                     ELYPDPAYQMNLTQIQSGNALLNGQAPESVYGLWSAPGARYNSYSKYDANLFGLSGKL
                     NMDFGHHSLQVGFQYEQRDNYSIEYAPVGLWTLARQISNKHIDQLDTTNPILRYDVNG
                     NFLDTIDFDRLYDANNQAFFDYNVRQYLGLPVDGTDWLDIDSYDPAMFQIDWFSADEL
                     LNNGNSYVAYYGYDYSGKKQKTRASFDDFFTQKDEYGNYTRPIPSFQPIYMAGYIQDK
                     FAAFEDLIFNIGIRVDRFDANQKVLKDPFLFYEAHTLREVRQLQPEWDHPGNMGDDFV
                     VYVDDIRNPSKPIGYRSGYTWYNAQGIEISDPTLLRTASGIAPYLVNPNLTHPTSAAF
                     KDYDPQITVMPRIAFSFPISEEALFLAHYDIITKRPTEGLRLDPTNYYFAQTRGGSIF
                     SNPALKPEKTIDYEVGFQQMIGEFSAIKLSTYYKEVRDLVQVYQFFGAYPIDYMSYNN
                     IDFGTVKGFTITFDQRRSKNIWMKASYTLQFAEGTGSSATSGYNLISTGQPNLRTIYP
                     LDYDQRHAFSVVFDFRYGSGKNYNGPVIKRKKEENGEIKVKETRVLENFGINVQFLGG
                     SGTPYSRSSNVVSALLGGGQYILLGSINGSRMPWSFTVNLRIDKEFYINVGKKSENSN
                     KKMYLDVYLEVLNLLNTKNIIAVYRATGNPDDDGYLSAPEYQAGIQAQLDEQAFRDLY
                     RIAVDSPYNYSLPRRLRLGVQIGF"
     gene            complement(9291..9599)
                     /locus_tag="PKI65_06195"
     CDS             complement(9291..9599)
                     /locus_tag="PKI65_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78067.1"
                     /translation="MEIILFILFILFIFFVLYIYSFKTKKQSSNAQKQNANNYQETKI
                     NEIKKDDNSEIFQSINEIDDIEEKVKNKAKTKENDIFSNGFDPKEGILYTEIFTKKYF
                     "
     gene            complement(9966..10661)
                     /locus_tag="PKI65_06200"
     CDS             complement(9966..10661)
                     /locus_tag="PKI65_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOB78068.1"
                     /translation="MKNISDNAIFDNLAPDYDSWFFTDAGKIVYQLELNALLQALKLE
                     KDCQILDIGVGTGIFSAELVKRGAIVVGIDPSEEMLSIAKQRGINEIVKGNGENLPFA
                     DEQFDIVLAFTSLEFSPNPQRFVSEMYRVCKPQGQIVVAVLTKWSMYGIARSISRLFR
                     KSIFRHTKFYTYSSLNKLLNPYVSNINYLSTVFINPKPPKFILKQVKKIEKFGQKFFN
                     KNGALLMMWGEKK"
     gene            complement(10642..11178)
                     /locus_tag="PKI65_06205"
     CDS             complement(10642..11178)
                     /locus_tag="PKI65_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOB78069.1"
                     /translation="MNNYENLKDRVEIYLGDITKLNVDAIVNAANTSLLGGGGVDGAI
                     HRAAGPELKEYCKTLGGCKTGEAKITPGFRLPAKYVIHAVGPVWRGGDFREDDLLKNA
                     YLNSLKLCKKYNIKTVAFPNISTGVYGYPLDRAAEIVWQTVLDWLSREPLPEKVIFAI
                     FSEDNYHIYKEYYEKYFG"
     gene            complement(11171..12526)
                     /locus_tag="PKI65_06210"
     CDS             complement(11171..12526)
                     /locus_tag="PKI65_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOB78070.1"
                     /translation="MNHIKYIIIFFVFLFSLNINYANDVDTIKKIYYFKLFSNIDQSA
                     RRLVNKAISDANDVNADLIIMHLNTYGGTLVDADSIRTAFLHSRIPIWVLVDNNAASA
                     GALISIAANKIFMVPGSTIGAATVVDQTSQALPDKYQSYMRAMMRSTAIARGRDPNIA
                     EAMVDPDIYIPGIIDSGKVLTFTAQEALKYGFCDNIVQGLDDLITKNNLQNAQIIKMR
                     VTWLDKVINFFLNPLVSAILIMLIIAGIYFELQTPGIGFPLILAILAAVLYFVPYYIE
                     GLAANWEILIFIIGIILVLVELFAIPGFGITGISGIILILAGLILTLVDNKGFHFPEH
                     ALEQLGTAFVTVTIAVFVGFTASILLSKRLFTQSVFGQNLSLTTEMKSENGYISSDLV
                     FKNLIGQTATTHTVLRPSGKIIIGDDVYDAICLFSYIDKDKKVKIIDYQNNQLIVEPY
                     E"
     gene            complement(12552..12640)
                     /locus_tag="PKI65_06215"
     tRNA            complement(12552..12640)
                     /locus_tag="PKI65_06215"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12604..12606),aa:Ser,seq:cga)
     gene            complement(12741..13313)
                     /locus_tag="PKI65_06220"
     CDS             complement(12741..13313)
                     /locus_tag="PKI65_06220"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOB78071.1"
                     /translation="MLSKISKYNLEAGVDEAGRGCIAGPVFAAAVILPENFSHPLIKD
                     SKKLTALQREEARKIIEANAISYAVAHISNEIIDEVNILQATMMAMHRALALLAIQPQ
                     FIVVDGNYFIRYRDLSYQTIKKADNTYCHVAAASILAKTYRDEYMTMLHDDYPMYQWS
                     KNKGYPTKLHRQAILDFGISPYHRKSFKLL"
     gene            complement(13347..>14062)
                     /locus_tag="PKI65_06225"
     CDS             complement(13347..>14062)
                     /locus_tag="PKI65_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB78072.1"
                     /translation="CGKSDASITLNVTGATPPYTYIWSNGSVDAMLNNISAGNYFVTV
                     TDANGCSSFEIINVNDLPGPTANFAYKPHITTIENPIIYFENYSFGATSYYWDFGDGT
                     SSEEVEPIHKYLEPRSYHVVLYAKDEYNCIDTIGADIIIKDISTFYLPNAIMPYRKNI
                     NNNFHIYGVNIDSDNFEMRIFDRWGKEIFYTRDINQGWDGTYNGEIVPQGVYSYIIKF
                     ITKTDGIKHSILGRIAVIH"
BASE COUNT         4731 a         2588 c         1911 g         4832 t
ORIGIN      
        1 gcactttgtg gactttgtgt aataacttgg tgttctctgt ggtaaaaaaa atcaaactaa
       61 ataatttacc acaataaacg ttatagaacc ataattttgg ttcataaatt aaattacaat
      121 caatattctt aaaaattttc atttactctc tcaccacctt atcactattg tcttgttatc
      181 ttataatgta attatcatac aattagaaat ttttgagaat attcagttat aatgtctaaa
      241 atttataatt caagttttta ttcacatata acaaaacaat aaacatatat aactataaaa
      301 aaagcgagct aattaaatta atttaattag ctcgcaacaa tattaaaaat tataaataat
      361 tataacataa gtttaacacc tatttgatgt gtaccttgaa atggatcact tattctataa
      421 gaatattcga aacctaagaa tgaagatttt tctttattca gaggtgcttg tactgtagca
      481 ccaaaagctg gtccagtata cgcagtagct ctatcatcag tgcttgtaat tcctttttca
      541 tagcagtatg atccacgtac cataaaaata tttttaaaat tatattctag acctaatgca
      601 aattgatctc gtgaaaaagc gttagagata aaagaaccgc caatagataa gaggtgatct
      661 tctacatctt cttgtaaatg gaagatataa ccagcactga tcaaaagttg cgtgggtagc
      721 tcaaattttt cagatctatg ttctaaggtc atagtcatat cactaccaaa aacatttcca
      781 cgaaatgaaa atccatcacc actaaaacgt aaagctggtc caatatttcg taatgctata
      841 cctaatctaa tattttcatt tgaactagga atatactgta tgcctgcatc tatggctaca
      901 ccggtagcat taagatctgc aatagcttca ttaactattt taacacttgc acctacgtgt
      961 atagaactag aaaatgaatg tgcaaaacta gcagtaacat ttaaaaagtt aggagtgtaa
     1021 taacctatac caccctccgg tttatctact gtggttatca ttatatcacc gaaactcatc
     1081 gtcataactc ctaatgctaa tacactattt ttttgttttc ctatcttagc actaaaacca
     1141 aaagcattaa ctgttacacc agaacctttg agccattgag tattagcaaa agcaacgtct
     1201 atcttattag taaaagctat acctgctgga tttaaatata cagcttcgaa accactcact
     1261 tgactcatat tagccaagcc ccaaccagca cttcgaggaa atggatttat caataattca
     1321 gaagctcctg cttcgcctac tctatcttca ttgcccgcaa acataaataa aggtactaat
     1381 atgaagatag tcaaaactga aataaatttg tattttttca tattctatta ttttaaaaat
     1441 taaaatgcat ttaagtcaat aggtctaaga gctccaaacc atttaactac cttttcgcct
     1501 ataccaggag catttacgtg aattatgtat aagccaccag caattggtat accagcatga
     1561 ttttttaaat cccaatctac aaaagtaatt tctggatcat ccttagtata tcttctaatc
     1621 aactgaccat tggtagaata tatacttact gtacattttt caggtaaatt ggttattcgc
     1681 actctattat ctacttgatt aatttcatac gcattccacg cataataagg attaggtact
     1741 acgtttatca aatctaatgc atctttagca gtttgtaaat cgccttttat tgttgttaaa
     1801 ttttctgtac tgaaagaata cattggtcta ttatcatttt gaggattagt attagcagaa
     1861 gcgggagtag aataatataa atcataagga cgcttaactc taaaacgtaa tcttacttca
     1921 ttatctaacc aattttcatt ctctactgaa agtggaatac caacccacaa tgctgagttc
     1981 catacttttc ttctatcagt taagctagta gaatttaatt tattatatat ccattgacct
     2041 tcatcgtaag ctggtggata attatcttga aaatcattat caccaatatg tcctattata
     2101 taaacaaaat gcttaccacc caataatagc tcaccaaggt tagtcattat cctatgagta
     2161 ggattgaaaa tcatatctct tccattctcg cctgccaacc atgaatcttc accaaaaatg
     2221 atatttaatc tttcaccagt ttctacatta atagcatatc ctgggaacca acccatgcct
     2281 gtaggtttat cataattagg atctggattg ccgtttttat caacagatgt agcctttcga
     2341 ggtgccattt tagcagctcc cccctgagct aaattaatat catcacatgt ttctatcacc
     2401 acacatctag tccatttatc tttatctggt gtaagaacta gatctacact tgctaaatta
     2461 gaaagtttat ttaaacttat ataagaagcc attccactag caataggatg attagcacca
     2521 gaagatacta atctataagg tgcccatgta ccatttaaaa ctttttcata atattgtttt
     2581 ggatctaacc atcccgaagg atcataatca tttaaagatt gatcatcaac attatgtaat
     2641 gtaccagaac gtatccagtt taatgaactt gaaccctctt catcaggcac accacttaac
     2701 catctacgag tactatcttt atattccatt gtagcttcta aaaatccatt tgttggatca
     2761 ttttcgtcac caggatataa gcattgttct attgtaatgg atatacctaa atccaaaaat
     2821 aattgttcat tacgattaga tatggctttt tgtgaattaa acttacctat taacgtactc
     2881 caatccggac tatacacttc tacttcccaa tcacaattac cttttattag attactctga
     2941 gaaacatttt tgttaaattt taaaatataa cgagcatcaa ttacatttaa aggatcaacc
     3001 acactaacat ttataggacc tcttccagtt ttatatttca attcttgtac aaaattatta
     3061 gctaaaattt ctttttcact ttcgggtaaa atgtctagta tatttccacc attaccttgc
     3121 ccttctatcc tagtaatttg cacgccatct ccataatttg ctctaataac agtaccacta
     3181 ttctcaggtt cagtaatatg cggtatagcg gtataaactt gaatattttt acgtcccatc
     3241 agataaggtt tagcctgacc atcaatagaa ccagcagagc caggaatatg tgaatatttt
     3301 ttatattcat tatatgcata agctacaacc atgtagtaat actttttatt attcactagt
     3361 cttttatctc cactagcaaa ttgatcttct acaatacgaa aagaatgctt gattccttca
     3421 ttttgtccat ttacttcttc cactggcatc atagcatcta agtcttcact atagtagaaa
     3481 ttgataagtc tcgagacaga atctttaaca tcacactgag caactaatct agctttagca
     3541 gcatcatgca attcagctgg tcctacagta gcatccttga gttgaaagat ctgataccct
     3601 tgaaatctat atactgaatc atatttttga tctggaggag tgcctatagg agaaacgata
     3661 gatggatcta cttcagcata atcttcatta gcattattag aagttggttt attagtaata
     3721 aaaagtatta attctctatt taattcttga ataactaaat cgggagcatc aggaccatct
     3781 agaactttaa aacaattctc aaataaacgt tgacatttat catcaacttt tcttaataat
     3841 tctactgatg caaaagggcc accgctagta gctctagccc aaggaatacc tacggttaca
     3901 taatttaatg cacctgggcg caatgtaaaa ggacctgcag attgcataaa acgacggtcg
     3961 tgaggtagat tacctgcggt ttcttcagtc caatatctag gtccattagg tggtaatccg
     4021 ccagtacccc aatcacaagg atcagtatca ccaggaaaca taaaatcaca ctcaggtcca
     4081 taggccccac tagaaatatg agcattgcca ccatacatca tctttgtatt gtctttccat
     4141 atacctttca aaaaattata atattcaata gctatatcag ggtctgtcat ataatttgga
     4201 actccagtgt tattatgata aacaaaacgt cgcataccga aacgttcatt atctacaata
     4261 ccatcaccga aattgactcc attaatgctc tcatcacata tttgttcata aatagtattc
     4321 ccatcagcat cagtatatat attgtattta ggattatcaa tgccatcagg atccatatat
     4381 ggaccttgga aaaagtcaat tcctatagca ggaggttgat cgccataagc ttctatttga
     4441 cctgtaccat ctacagcttt accattataa caatacccta aacctctctc tacatcacaa
     4501 cctaaatagt catcccatgc atatcctaga tcggggtccg tccataatga catataagtt
     4561 tcatacaaag tataagtaga tctattaata atctcgaagc tgtaaaaagt catattatta
     4621 atttcatcat tagtagtgaa agcaaaagct tgagcacgta tttcgaagcc aatttgttgc
     4681 ccttgtgttt cagtatgtat attacctcta tcattaaaaa cccagaaaag cgtttgatca
     4741 cccttcaaaa cttggtctgc cataatacca ccaaatacat atcccatttc agtttctaat
     4801 gtaggtaact cttgtttaca taaactatta tctatatcat aataaggata atcaccatca
     4861 taaggattat aagtaccatc accattatga tcgtaaaatg gagctatata aaaagattga
     4921 cctttagtag gatcaccatg agctggataa tcaaagaaat attttggtat cggataagat
     4981 gatatattag cagaatcttc tgtccaccaa gctaaaaatt gatctacatc tttacgataa
     5041 atagtccaaa ttttgtccca tctagtacac tcatcagcat caatgaaagc tgctccatcg
     5101 gtagaaagag gaccaggaaa gaaatcgacg ccaatttggc gatatctctg tgcggcgagc
     5161 tttagctgcc cattaacgtc tatccctccg atccatagag caccagcaaa catagaagtt
     5221 tttttagaac cttttggtat ttcatattga gctgtaccac gacctccagc agtttgaaga
     5281 tcccaccaca tatcaccacc actatttatt cgcgttctca cattatttat atttaactcc
     5341 gtataaaatg cacctgctag acatcctgca gcagcctctt ttaattcatc agaactcatt
     5401 gttcttttat taatcccagc cgtaggaaca ttttcaccga ctacaagacc tacaaaaagt
     5461 gccaggacaa ttgatacaaa tacttttttt atcatattta ttattttttt aaaattaaaa
     5521 acctatttgt acacccaaac gtagacgtct tggtaaacta taattatatg gactatctac
     5581 ggctattcta tacaaatctc taaatgcttg ctcatctagt tgagcttgta taccagcttg
     5641 atattcaggt gcagagagat atccatcatc atctggatta ccagtagctc tataaactgc
     5701 aataatattt ttagtattca ataaattcaa gacttcaaga taaacatcta aatacatttt
     5761 tttattagaa ttttctgatt ttttacctac attgatgtaa aactctttat ctattcttaa
     5821 gtttacagta aaagaccacg gcattctaga accatttata gaacctaata aaatatattg
     5881 tccaccacct aggagtgctg atactacatt actagatcta ctataaggcg taccactacc
     5941 acctaaaaat tgaacgttta taccgaagtt ttctagaact ctagtttctt ttacttttat
     6001 ttcgccattt tcttcttttt ttctttttat aacaggacca ttataatttt ttccacttcc
     6061 ataacgaaaa tcaaatacaa cagagaaagc atgacgttga tcatagtcta aaggataaat
     6121 agtacgtaga ttaggttgtc cagtcgatat taaattatat cctgacgtag ccgatgatcc
     6181 agtaccttcg gcaaattgta gagtgtagga agctttcatc caaatatttt tggaacgtct
     6241 ctggtcaaaa gttatagtaa aaccttttac agtaccgaaa tctatattat tatatgacat
     6301 ataatctata ggataagcac caaaaaattg ataaacttgt accaaatctc ttacttcttt
     6361 ataatatgta cttaatttaa tagctgaaaa ttcacctatc atttgttgaa agcccacttc
     6421 gtaatcgata gttttttcgg gctttagtgc tggattacta aaaatggatc caccacgtgt
     6481 ctgagcaaaa taataattag tagggtctaa tctaagacct tcggtaggtc tcttagttat
     6541 aatatcataa tgagctaaaa ataaagcttc ttcggaaata gggaatgaga aagctatacg
     6601 aggcatcaca gttatctgtg gatcataatc tttgaatgct gcacttgtag gatgagtcag
     6661 attaggattt actaaataag gagctatacc actagcagta cgtaataatg tagggtctga
     6721 aatctcaata ccttgagcat tgtaccacgt ataaccgctt cgataaccaa taggtttgga
     6781 aggatttcta atatcatcta cataaacgac aaaatcatcc cccatattac caggatgatc
     6841 ccattcgggt tgtaactgtc tgacctccct cagagtatgt gcttcataaa acaagaaagg
     6901 atctttcaat actttttggt tagcatcaaa tctatctaca cgtataccaa tattaaaaat
     6961 aagatcctca aaagctgcaa atttatcttg aatatatcca gccatataaa taggttggaa
     7021 agaaggtatt ggcctagtat aattaccata ctcatctttt tgtgtaaaga aatcatcaaa
     7081 gctagcacgt gttttttgtt tcttaccact ataatcataa ccataataag ctacatagct
     7141 attaccatta tttagtaatt catcagcact gaaccaatct atctgaaaca tagctggatc
     7201 ataagaatca atatccaacc aatctgttcc atctacaggt aaacctaagt actgacgtac
     7261 attataatca aaaaaagctt gattattagc atcatataat ctatcaaaat caatagtatc
     7321 taaaaaattg ccattaacat cgtatcttaa aataggatta gtagtatcta actggtctat
     7381 atgcttatta gatatctgtc tagctagtgt ccataagcct actggtgcat attctatcga
     7441 ataattatct ctttgttcgt attgaaatcc tacttgcaat gaatgatgtc caaaatccat
     7501 atttaattta ccacttagtc caaatagatt agcatcatat ttactatagc tattatacct
     7561 cgcacctgga gcagaccaca atccataaac gctctctgga gcttgaccat ttaaaagtgc
     7621 attaccactt tgtatttgtg tcaagttcat ttggtaagcg ggatctggat aaagttcata
     7681 aaatctggaa gtataagcag ctaaagctgg attagctgta cctggttcga aatcaacgtg
     7741 agtatcataa aaattatctt gcacccatac attattcaaa tctaataaag gataatattc
     7801 cataaacgca taggatctag ctttagtagt ggtaaatttt cccacataac catatttgaa
     7861 taaatcatct ttaaaatcag gatcttgtat tacttgattt attttactat aatcagcttg
     7921 caagctatag tatatattac ttattaattt tttagtagta tctttacttt ctaatgatct
     7981 acgagtaaaa tcttgactaa atctaacata agctctacct tcataagtta atatctgtgg
     8041 attatttttc caattaaata aactaaaact atgtacatat cccatagaat tggcatatct
     8101 aaaagatccg ccaaaagtta attggatatt tggacctgta ttaacagtaa ttttagcaga
     8161 taaatttact tgcttagcat cagcatttag tttagctttt atatgttcta aatctttcat
     8221 gcgaataaaa gcagcattag gatgtgcacc gaaatcacca tattgcgaag gacgcaatgg
     8281 atttttttct aaatattcaa ctacatcatc cttagcttta tataaacctt tagcaaaagg
     8341 tcttgggtct ttttcgtagg aaagctcacc agctaagaaa aagttaaaaa ttgatcgttt
     8401 aggatctttt ttacttttaa ataaaggtcc ttccaagcta aaacctaata aattataacc
     8461 ataaggatcc aaaaattggc tagtaactag ctctatacct ccgcttaatt tggtactacc
     8521 agatttagta gtaatattga taactccacc tgtagcctct ccatattcag caggagtacc
     8581 accagtgatc actgttactt gatctaatgc tgattttgga agattagtag tacctatagt
     8641 tctaatacca tctatatata tcaccgtacc actctctcgc tggcctctaa tattaacatc
     8701 agtactacca tcatcttgtt taaaaacacc agcagcagtt tgagctacac cgatagcgtc
     8761 acgagtaggt aatttactaa tttcagtagc tgtcatggta gtagcagtcg acacattacc
     8821 tttgtctata ggtttatctc taaaaccaac tacttctact acatcgagta cttctgtttt
     8881 taaagatatt ttttcattta tataagtaat tttatcaggt gaaataacaa cgccctctat
     8941 caccttggta ttgtaagaaa ctgcactgat acgaatatta tatgtaccag ggggcaccgg
     9001 tgctatttta tatctaccat ctatatcagt aacgcctcca gtgacaatcc gctcaccact
     9061 cataatagct acgtgagcaa atggaacagg tttattagtg ccttcttctg taatagtacc
     9121 cattattgta ccagaagact gtgctaatgc caaaaaacta attagcatta aaaaaactaa
     9181 agataataat cttgctcgca tattatttta ttttttatat tttaattttt atttcgttgg
     9241 ctaaattaaa aaaatatttt aatacatatt caaacatatc caaaaatttt ttaaaaatat
     9301 ttttttgtaa aaatttcagt atataaaata ccttctttag ggtcaaatcc attagaaaaa
     9361 atatcatttt cttttgtttt ggctttattt tttacttttt cttcaatatc atctatttca
     9421 tttattgatt gaaatatttc actattatca tcttttttga tttcattaat ctttgtttct
     9481 tgataattat tagcattttg cttttgagca ttgcttgatt gcttcttggt tttaaagcta
     9541 tatatgtata aaacaaagaa aatgaataaa atgaatagaa taaataaaat tatttccatt
     9601 tgatttattt ggctacgaaa ttaaaaataa tatttttaat aaaaaaataa ttttgataaa
     9661 taatttttat tttcatccgt tttttaattt tcatacgaca aatattattc taatttttgt
     9721 gatttacatt gattttaaaa gataaaatac atattatatt attttattta tttctataac
     9781 atttattgtg gtaaattatt taggtttttt taccacaaag agtacgaaga tattatacaa
     9841 agtttatcct gtttaataat aaataaacaa atattaacaa attactcttt ttaatcttta
     9901 tacatataaa acgttataga accttttatt tttattacat aaatatttat gagcaatttt
     9961 attaattatt ttttttcacc ccacatcatt aataatgctc catttttatt aaaaaatttt
    10021 tgaccaaatt tttctatttt ttttacttgt tttaaaataa attttggcgg tttaggattt
    10081 ataaaaactg tgcttaagta attgatattg ctcacataag gatttagtaa tttattaaga
    10141 gatgaataag tataaaattt agtatgtcta aaaatactct ttctaaataa tctacttata
    10201 gatctagcta taccatacat agaccactta gtcaatactg ctactactat ttgtccttgc
    10261 ggtttacata ctcgatacat ttcagagacg aaacgttgag gatttggaga aaattctaaa
    10321 gaggtaaaag ctagtactat atcaaactgt tcatctgcaa atggtaaatt ctcgccatta
    10381 ccctttacta tctcattaat gcctctttgc ttagctattg atagcatctc ttctgatggg
    10441 tctatgccta ctactattgc gccccttttc actagctctg ctgaaaatat gccagtacct
    10501 actcctatat ctaatatttg acaatctttt tctaatttta aagcttgcag tagagcattt
    10561 aactctaact gataaactat tttgccagca tctgtaaaaa accaactatc gtagtctgga
    10621 gctaagttat caaaaatagc attatccgaa atatttttca taatattctt tgtagatatg
    10681 atagttatct tctgaaaaga tagcaaaaat aactttctct ggtaaaggct cacgtgagag
    10741 ccagtctagt actgtttgcc aaactatctc tgctgctcta tccaatggat aaccataaac
    10801 tcctgtacta atattaggaa aagcaacagt cttaatatta tattttttac atagttttag
    10861 agaattcaaa taagcatttt ttaataagtc atcttctctg aaatcaccac cacgccatac
    10921 tggtcccact gcatgaatga catacttagc tggaaggcga aaacctggag ttattttagc
    10981 ttcgccagtt ttacatccac cgagagtttt gcaatactct tttagttctg gaccggctgc
    11041 tctatgtatt gcaccatcta ccccaccacc acctaatagt gaagtgtttg ctgcatttac
    11101 tattgcatct acattcaatt ttgtgatgtc tccaagataa atttctactc gatcttttaa
    11161 attttcataa ttattcataa ggttctacta ttaattggtt attttgataa tctataattt
    11221 ttactttttt atctttatct atgtaagaaa acaaacaaat tgcatcatac acatcgtcac
    11281 cgataattat tttacccgaa ggtctgagta ctgtgtgggt agtagctgtt tgaccaatta
    11341 gatttttaaa aaccaaatca ctactaatat agccattttc acttttcatc tcagtagtta
    11401 gtgataaatt ttgtccaaag acagattgtg taaataatct tttagataaa agtatactag
    11461 cagtaaaacc gacaaataca gctatggtta ccgtaacaaa tgctgtacct aattgctcta
    11521 gagcatgctc tgggaagtga aaccctttat tatctactaa agtaagtatt agtccagcta
    11581 aaatcaatat tataccgcta attccagtaa tgccaaagcc aggaatagca aataattcta
    11641 ctaaaactaa tattatgcca ataataaaaa tcaaaatttc ccaattagca gctagtcctt
    11701 caatataata tgggacaaaa tacagcacgg cagctaaaat cgctaatatt agcggaaacc
    11761 ctatccctgg tgtttgcaat tcgaaatata tacctgctat tattagcatt atcaatatag
    11821 cacttacaag aggatttaga aaaaaattta ttactttgtc taaccaagtt actcgcattt
    11881 taataatttg tgcattttgt aaattatttt tagtaattaa atcatctaat ccttgtacta
    11941 tattatcgca aaatccatat ttgagagctt cctgtgcagt gaaagtaagc actttgccag
    12001 aatcgataat gccaggaatg taaatatctg gatctaccat agcttcggct atattaggat
    12061 ctctaccgcg agctatagct gtactacgca tcatagctcg catatagctt tgatatttat
    12121 caggtaatgc ttgtgaggtc tgatctacaa cggtagctgc accaatagta gagcctggca
    12181 ccataaatat tttatttgca gcaatagaaa ttaacgcacc agcggaggct gcattattat
    12241 ctacaagtac ccaaataggt atcctagagt gtaaaaaagc tgtgcgtata ctatcagcat
    12301 ctactaatgt gcctccatat gtattcaaat gcataatgat gagatctgca ttcacatcgt
    12361 tagcatcgct gatagccttg tttactaacc tacgagctga ctgatctata ttagaaaata
    12421 atttgaaata ataaattttt tttatagtat ctacatcatt agcataattg atatttagag
    12481 aaaacaaaaa aacaaaaaaa attataatat attttatatg attcataaaa tagtaataaa
    12541 taaccattta gcggagagag agggattcga accctcggtc cgtcgaataa cggacaacgg
    12601 ttttcgagac cgccccattc gaccgctctg gcatctctcc taattattga tttattacaa
    12661 aattataata taaaattgta atgaattttt tattataata aaaaatgcta aaaacttacg
    12721 gatgaatttg tcaagatcaa ttataaaagt ttaaatgatt ttctgtgata tggacttata
    12781 ccaaaatcca aaattgcttg cctatgcaat tttgttggat aacctttgtt ttttgaccat
    12841 tgatacattg gataatcatc gtgtagcata gtcatatatt catctctgta agtttttgct
    12901 aaaatagaag ctgctgccac atggcaataa gtattatcag cttttttgat agtttgatat
    12961 gataaatctc tatatctaat gaaataattg ccatctacta cgataaattg tggttgtata
    13021 gctaatagag ctaaagctct atgcatagcc atcatagtag cttgtaatat atttacctca
    13081 tcgattatct cattagatat atgcgctaca gcataactaa tagcattagc ttcgatgatt
    13141 tttctagctt cttcacgttg taatgcagtt agttttttag aatcttttat caagggatga
    13201 gagaaatttt ctggcaaaat aactgctgca gcaaataccg gaccagctat acaaccacgt
    13261 cctgcttcat ctactccagc ttctaagtta tattttgaaa ttttgcttaa caaagattaa
    13321 tttttttcaa aattattaaa atttttttaa tgaataactg cgattcttcc taatatagaa
    13381 tgcttaatac catcagtttt tgtaataaat ttaattatat aagaatacac tccctgaggc
    13441 actatttcgc cattataagt gccatcccaa ccttgattaa tatccctcgt ataaaatatt
    13501 tctttacccc atctatcaaa aattctcatt tcgaaattat ctgaatcaat attaacacca
    13561 taaatatgaa aattattatt gatattcttt ctatatggca ttatggcatt tggtaaataa
    13621 aaagtactaa tatcttttat tataatgtct gcacctatcg tatctatgca attatattcg
    13681 tcttttgcgt ataatactac atgataactt ctaggctcta gatatttatg aataggctct
    13741 acttcttctg aactagtacc atcgccaaaa tcccaataat atgaagttgc tccgaaagaa
    13801 taattttcaa aataaataat aggattttct atagttgtta tatggggttt atatgcgaaa
    13861 ttagctgtag gaccaggcaa atcatttaca tttataattt caaaactact acaaccatta
    13921 gcatctgtta ctgtcacaaa gtaattacca gctgaaatat tgttaagcat agcatctaca
    13981 ctaccattag accaaatata agtatatgga ggtgtcgctc cagttacatt tagtgttatg
    14041 ctagcatccg atttgccaca at
//
LOCUS       DAOPRN010000049        13403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_3879_length_13403_cov_109.012137, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000049 DAOPRN010000000
VERSION     DAOPRN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13403
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3879_length_13403_cov_109.012137"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..2324
                     /gene="recG"
                     /locus_tag="PKI65_06230"
     CDS             180..2324
                     /gene="recG"
                     /locus_tag="PKI65_06230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOB78073.1"
                     /translation="MNEINFLDLPLEVAITQLPQAEVKAKKITINKCKLLKKELEITT
                     IRDLINHFPYRFYDKREFKKIKEASHYFNQYILLTGYFKEFYEENLGKRRVMKGIFAD
                     ASGIIEITWFNHYGWVKEKIKTNIQYVLFGRVSYYKNYYIAHPEIKTLEKFLQSEEYK
                     HLYPIYSTTERLAKAGLNSDGIMSFIKIVLPQAMKYIKEPYPNELLKDAKLVSITEAY
                     QNIHFPKTEKDYEKALYRMKFSEAFDLQIFYAYQNVVRDKQQTPYRFTKVGKLFNEFY
                     NKHLPFELTKAQKKVIREIWEDLRSGRQMNRLLQGDVGSGKTIVALMSILLAIDNGYQ
                     ACLMAPTELLAQQHFKTISKLLKGLPVEVDLLVGSTPQKKRNYLLKDLLEGKINILIG
                     THALIEEVVQFKNLGLVIIDEQHRFGVIQRAKLHLKSDYPPHVLVMTATPIPRTLTLT
                     VYGDLRVSVIDELPPNRKPVKTIYLPSTLRYQAWDLMRNEIKKGHQAYVVYPLIDESK
                     KMNLIALTEGYDAIIAEFPPPQYSIAMIHGRMSSQERDEIMYHFSKGKIDILVATTVI
                     EVGVDVPNASLMIIENAERFGLAQLHQMRGRVGRSSEQAYCILIGGENISENAIHRLK
                     IMVETTDGFKIAEEDYRLRGPGEIEGVRQSGQRFFNIIDPINDQHLIEYSNKFAQELI
                     CKDPLLEKTEYKSLHSIIERYKLENISLSSIG"
     gene            complement(2353..3342)
                     /locus_tag="PKI65_06235"
     CDS             complement(2353..3342)
                     /locus_tag="PKI65_06235"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOB78074.1"
                     /translation="MLNIALMYGGYSGEREISIKSAEMVAKNIDNTKYEVYLIDVQRD
                     KWICKNKNGKETEILLNDFSANYKNNKINFDLAFILIHGTPGENGLLESYLEMKQIPH
                     TTCSAAVSMLTFNKYYCKTIVKDSGFVAIAPSVYFSHKIPIDEIKNKISQLNYPIFIK
                     PVNSGSSVGLSKIYSEDELNDAVHLAWKYDNEILIEQGINGRELTCGVFNYKGNIISF
                     PVTEIIPKNDFFDFEAKYNSGMSDEITPANISEDFSQNIRSISVNLYKYLSCFGVVRF
                     DFIWNEEELFFLEVNTIPGMSEASIVPAQAKAANIPLIKLTDMMIENALTRFF"
     gene            complement(3475..5271)
                     /locus_tag="PKI65_06240"
     CDS             complement(3475..5271)
                     /locus_tag="PKI65_06240"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78075.1"
                     /translation="MRSYCILLLLILIINPIFAQKKTKIIKTYPDGQIKEKGIISLPD
                     STRIGKWYFYFHNGQLSMEGEYDSNGLEIGTWKQYYPNGEIYIVEAKDNGPIKAYYKY
                     PDTLKMTGQLKNRKPEGEWIIYYENGNIQSRQYFSEGKMIGQWEYYFENGKLWKIISS
                     DTGPVIIYDSLGNKIEEWHIVNGQMDGEHKTYTTHGTLISKITYKNGKKDGPYELYHG
                     NGQKALEGFYKDDKKIGIWKYYYPNKKLAKEENQETGPCYSWYENGNLNEKYFHQNGK
                     KQGLYLSYHLNGKVKDSIYYDNGLRQGKVISYYDNGNKEFEGYYINDSLNGYCYWWYY
                     NGFMEMEGKIVNDKHDSLWKFYHPTGKLGSIGMFVNDKPDGLWTYYYEIGKKWREGYY
                     KNGLREGKWVAWYESGKKQREGNFINDREDGYWVSWYENGNVQHEGYFKNGLMNGPWK
                     GYYSDGSLKYQTNYTDNVKNGSDVFYLPNGNKLYDGAYKMGLKDGYWVEYNEIGKIAA
                     EGVYKNEKREGLWKFYNQYGGLEREQFFKNGLPDGKFVEYHPNHQIATIGYYKNTAKV
                     GEWILYDKTGKIIAKMFFDSNGKLLKKVTSID"
     gene            complement(5334..9833)
                     /locus_tag="PKI65_06245"
     CDS             complement(5334..9833)
                     /locus_tag="PKI65_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78076.1"
                     /translation="MKKISFIFITVFILFSINLHAQITQFTQDPTTYISGLEQLFRQD
                     RNISKSTLKEIDAFLLGEFSTFWSSITDLEKQSIIELSNAMLKKRLGSEHFLSFLHSL
                     INIQKSGKWQSAYTAWSQTYKNIVEKERAQKIIQFLSQSDSLFLNAILYSSPTTTWMS
                     TSNDFHFEYDKEPKIIFPNTTLKCYAYGDSSVIYHTSGTYYMNNYKWEGKGGKITWQR
                     ALLDPEGVYAILSNYTISLKSRDYRADSVNFYHKDYFGDEPITGFLIERIFADKTGEQ
                     SIYPQFYSYQTNFYINNIFKNIDYEGGFTVKGASIIGHGDSISPATIRLFYDNKLFIK
                     SQSNSFSIRKNSIDSQDASVVIYLNEDSIYHMSLIFKYQDSTKEIMCIRDNERLHPMP
                     FYNTYHNIDMYFEALYWTIGSPEIRMSMMKGGSLGNALFESNNYFSKSRYLKLQGIDE
                     KNPLQYIKEFIDQTGKKYFSVLDFAKFRKIDIRIIRQQIISLALQGFVDYNPTTDMVY
                     AKDKIYDYIEANVGKKDFDVIQFNSVVESKDNARLSLINYDLKLEGVSRVFLSDSQNV
                     VVYPEAQMVIVKKNRDFTFNGVISAGRFNYFGREYYFSYEDFKINMPYVDSMNFYVRS
                     RVPDKYGEYPLVRVKNVIEDLSGEILIDHPSNKSGKIYMPEYPIFDSKKISYVYYDKS
                     CIYQDVYLRDDFYYHINPYRLENLDKFQTDSLKFDGYLASGGIFPDIYEPLIIMDDYS
                     LGIFHKTTSDGLPAYGGKGTFVNEITMSNQGLYGNGTLKYITSTAISKNLLFFPDSAK
                     GIVDSYVITERSTGIQYPPVNVENAKIIWYPKQDFMSINTIETPAIAYKENIKINGIL
                     DYSPLGLFVNGKIEFLNAIMNSKKFTLKNRVFDTDTCDFVLKTLDLNEFALETQIYKG
                     HVDFDKRKGSFETTGENSMIRFPANEYICFMDKFDWYMDKEELDFYTIKRKDVDYTHI
                     SSRDLVDMDLEGSIFISTHPAQDSLSFISGKANFNIRDKIITAYEVPYIQVADAAIFP
                     DNGIVYILPHAEMKPLENSKVLANLETKYHILHNCKIWIYGKKSYSGNGHYEYVDKNN
                     NSQLIYFNKIFVVNESQTTANGNVPDSVNFTLSPYFLYTGDVHLYADQEFLSFDGGIK
                     ISHNCDTLTKQWVKFNQMIDPTDIMIPIPAQPKNTSGENIFAGLYFSSKENAGVYPAF
                     LTKKLYGGDIELVSSHGFLKYDEPSNEYQITSKEKFDGLTMKGNYLSLNTHNCKVRGE
                     GKLSLGSNLGRVAMDFYGRVDHYIYSDSTIINGNINLDFFFNDKALDMLATDILGSDL
                     PGLNIQDELFLSFIEEIIDSTDAMNVIKDLNLYGNIRKMPKELMKTFVFNDVTFRYIP
                     ETKTYVSEGYIGISVANGKQVYKYVNGIIELSLRRSGDRLTIYIEPLPNVWYFFTYNN
                     GLMQGLSSRKEFNELIINTDSKSRILPSSGKEKAYSYYIATTTTKDRFLKKIYENIDT
                     GEGNY"
     gene            complement(10053..12278)
                     /locus_tag="PKI65_06250"
     CDS             complement(10053..12278)
                     /locus_tag="PKI65_06250"
                     /inference="COORDINATES: protein motif:HMM:NF018504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78077.1"
                     /translation="MPHKDENPLIPYDKTANELEYLLGLTKQIREKEQRYPINFNDFL
                     FLLSTNPKQVLRNIFQLFHDMVHYYVPEGTDEYENDSENVGFSFYDLSKLLVENVEVP
                     FFSDRLFANRFMQMAEEINKGINVNQIYLFEGPPGSGKSTFLNNLLQKMQEYTLTDEG
                     TVYKIFWKIDTKDINNKIENGKIISYSCPHNDNPFLIIPKELRKDFLKTLIKDPKVYE
                     DIIESKIYEWLFRSEPCSICKSIYNSLIDILEDPLQVYDMIYAKRIDFNRQLGKGISV
                     WNPADNLIEHIYINDNLQNELRNLLSTEEIECKYSYLAYTNNGIYAIMDIKDNNITRL
                     LSLHGIISDGVHKVEHIEERINTLFLGLVNPEDKKTFIDIKSFQDRVRFVLIPYVLDA
                     ETEVRIYENKFGSNIQKKFLPRVLQNFAKIIICTRLNTESDTLKRWLKHRSKYEKYID
                     PDLLLLKMELYHGRIPRWLEEEDIQGFKRNIRLAVLNEALDEGFKGISGRQSIALCNK
                     LLSTYQEKDEEIDMLDIINFFNDQPDSVKKLIPPNFINSLLKIYEINIVQELKDALFT
                     YNENKILEDIIHYLLALSYDIGAKVTNPFTDKTYIVTNEFLESIENRLFPEIVDRDKN
                     RNNLLNRFITETLAQEIEVKQKKWQHTQLIQDLFDLYKENIKKASLDPYLKNQSFRFA
                     LKDYFTDKFGNYDNKIQEDIRHMISVMCDKYGYSERGAIKITLYIIDRNLPERHKEYL
                     M"
     gene            12398..13315
                     /locus_tag="PKI65_06255"
     CDS             12398..13315
                     /locus_tag="PKI65_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78078.1"
                     /translation="MKPKLIIILFCLVLMFNSCAKTGNKKKDERKNNNVQNLSILIPI
                     YMEQPIKEWTYFLQASHPNVKFEIICMNEQYFSSDTTFRDFDLIFTPSFIRGNKYKYS
                     IISQEAIVCVVSFENPFLQSIINRGISPNDLKKIFSNPNSYYWKDFFKTDKKLNDKIT
                     IILPDDRYTLINQLFDWLDIDKPTSVTMKSTDSILDLLKIQNDIMTFLPSSLVYNINT
                     QYRKDFLYVVPIDLNNDNWISDDEYLYDNIIMIKKAVLDNKIDSLLVLQYKLIYSIDN
                     SRKNLIEDIVNNKHNLNKNIFEQYGYFVN"
BASE COUNT         4670 a         2083 c         1763 g         4887 t
ORIGIN      
        1 tacagggact tgagagctaa gccaatatta aatttaacat aaaaaaattt tatctcatct
       61 tacctcactc atcttatgtg ccaataaaaa ttgcattaca tttttgggat agaaatagtg
      121 attatttata taatatttta ttgttatatt tgaaatatat ttattcgtaa aaaagaaaaa
      181 tgaatgaaat taatttttta gatttacctt tagaagtagc tattactcaa ttgccacaag
      241 ctgaggtaaa agctaaaaaa ataacaatca ataaatgtaa attattaaaa aaggaactag
      301 agattactac cattagagat cttataaatc attttccata tagattttat gacaaaaggg
      361 aatttaaaaa aataaaagaa gcatctcact attttaatca atatatttta ttaactggat
      421 attttaaaga gttttatgaa gaaaacttag gaaaaaggcg agttatgaaa ggaatattcg
      481 cagatgctag tggtataatt gaaataacat ggtttaatca ttatggatgg gtaaaagaaa
      541 aaattaaaac taacattcaa tatgtattat ttggaagggt tagctattat aaaaattatt
      601 acatagcaca tccagagatt aaaaccttag aaaaattttt acaatcagaa gaatataaac
      661 atttgtatcc aatttacagc actacagagc gtcttgcaaa agctggatta aattctgatg
      721 gcatcatgag ttttattaaa atagttttgc ctcaggctat gaaatatatt aaggaacctt
      781 atccaaatga gctattaaaa gatgcaaaat tagtctctat cactgaagca tatcaaaata
      841 tacattttcc taaaactgaa aaagattatg aaaaagcatt gtataggatg aaattttcag
      901 aggcttttga tttgcagatt ttttatgctt atcaaaatgt cgttagagat aagcaacaaa
      961 caccatacag atttacaaag gttggaaaac tttttaatga attttataat aaacatcttc
     1021 cttttgaatt aacaaaagct caaaaaaaag taattagaga aatctgggaa gatctgcgta
     1081 gtggcagaca aatgaacaga ttactacagg gtgatgtagg cagtggcaaa actatagtgg
     1141 ctctaatgtc tatattgtta gctattgata atgggtacca agcttgttta atggcaccta
     1201 cagagctact tgctcaacag catttcaaga ctatttctaa gcttttaaaa ggtttacctg
     1261 tagaggtaga tttattagta ggtagtactc cacaaaaaaa gagaaattat ctattaaaag
     1321 atttattaga aggtaaaatt aatattttga taggaacaca tgctttaata gaggaagtag
     1381 tgcaatttaa aaatttagga ttagtaataa tagatgagca gcatcgcttc ggcgtgatac
     1441 agagagctaa actacatctc aaaagcgact accctccaca tgtattggta atgacagcta
     1501 caccaatacc acgcactttg actctcacag tatatggtga cctcagagtt tctgttattg
     1561 atgaattgcc acctaatcgc aaaccagtaa aaactattta tctgccttct actctacgct
     1621 atcaagcttg ggatctgatg agaaatgaaa tcaaaaaggg ccatcaggca tacgtcgttt
     1681 atcccctcat agacgaaagc aaaaaaatga atttaatcgc attgacagaa ggatatgatg
     1741 ctatcatagc tgaatttcca cctccacaat acagcatagc catgatacat ggacgtatga
     1801 gttcgcaaga acgtgatgag ataatgtacc atttctctaa aggcaaaatt gatatattag
     1861 ttgctactac tgtgatagaa gtgggagtag atgtgcctaa tgctagtcta atgattatag
     1921 aaaatgctga aagatttggt ttagctcaat tacatcaaat gcgtggcaga gtaggcagat
     1981 cttctgaaca agcttattgt atattgatag gtggagaaaa tatcagtgag aatgctatac
     2041 atagattgaa aataatggta gagactactg atggattcaa aatagcagaa gaagactatc
     2101 gtctaagagg cccaggagaa atagaaggtg taaggcaaag cgggcaacgt ttttttaata
     2161 ttatagatcc aataaatgat cagcatttaa tagaatattc caataaattt gctcaagagc
     2221 ttatttgtaa agatcctctt ttagaaaaaa cagaatacaa atcattgcat tctattatag
     2281 agagatataa gttagagaat atttctcttt catccatagg ataaaacttt agtataattt
     2341 tgaaattaaa tttcaaaaaa atcttgttaa agcattttct atcatcatat cagttaattt
     2401 tattaatgga atattagcag cttttgcttg tgcaggcact atagatgcct cgctcatccc
     2461 tgggatagta tttacttcca aaaaaaataa ttcctcttca ttccagatga aatcaaatct
     2521 aacaacaccg aaacatgaca aatatttata taaatttact gatatagaac gaatgttttg
     2581 agaaaaatcc tcactaatat tagcaggtgt tatttcatct gacataccag agttatattt
     2641 agcttcgaaa tcaaaaaaat catttttagg tattatctca gtaactggaa aagaaataat
     2701 attgccctta taattaaaaa ctccacatgt gagttctcgc ccattaattc cttgttctat
     2761 taatatctca ttgtcatact tccatgccag atgcacagca tcatttagct catcttcact
     2821 ataaatttta ctgagcccca cactactacc actatttact ggttttataa atataggata
     2881 atttaattgt gatattttgt tttttatctc atcgataggt attttatgag aaaaatacac
     2941 tgatggtgct atagctacaa atccgctatc ttttacaata gttttgcaat aatatttatt
     3001 aaaagtaagc atagatacag cagcactaca agtagtatga ggtatttgct tcatctcaag
     3061 atagctttct aataaaccat tctctcctgg tgtaccatgg ataagtataa atgctaggtc
     3121 aaaatttatt ttattatttt tataattagc cgagaaatca ttcaacaata tctctgtctc
     3181 cttaccattt ttatttttac aaatccactt atctcgctgt acatcaatga gatatacttc
     3241 atatttagta ttgtcaatgt ttttagctac catttctgca cttttgatgc tgatttccct
     3301 ttcacctgaa taaccaccat acattaaagc aatatttagc atcgttctaa atttttacaa
     3361 aattaaaaag aaaaaaatta taaggttaaa aaaaaataaa cgaaatgact taatgatatt
     3421 aaaagaattt ggaataatct aaaataatat aaatttagat atcaaaagct tacttcaatc
     3481 tatagaagta acttttttta gcaatttacc attactatca aaaaacattt tagcaataat
     3541 tttaccagtt ttatcataca gaatccattc acctaccttt gcagtatttt tataataacc
     3601 tatcgttgct atttgatgat ttgggtgata ttctacaaat tttccatcag gcaatccatt
     3661 tttaaaaaat tgctctcttt ctaacccacc atattgatta taaaatttcc acaaaccctc
     3721 acgtttttca tttttatata ctccctcagc agcaatttta ccaatttcat tgtattcaac
     3781 ccaataacca tcttttagtc ccattttata cgctccatca tataatttat taccattagg
     3841 gaggtaaaaa acatcagaac cattttttac attatcagta taattagttt gatattttaa
     3901 tgaaccatca gagtaataac ctttccacgg gccattcatc aatccatttt taaaataccc
     3961 ttcgtgttgc acatttccat tttcgtacca gctaacccaa tatccatcct ctctatcatt
     4021 tataaaatta ccttctcttt gttttttgcc agattcatac caagccaccc atttgccctc
     4081 tctcaatcca tttttgtaat agccctcacg ccatttttta cctatttcat aatagtaagt
     4141 ccatagtcca tctggcttat catttacaaa cataccaata ctgcctaatt tacctgtagg
     4201 atgataaaat ttccataagc tatcgtgttt gtcattaaca attttccctt ccatttccat
     4261 aaagccatta taataccacc aataacagta cccattcaaa ctatcattta tataataacc
     4321 ttcgaattct ttattaccat tatcataata agaaattacc ttaccttggc gcaaaccatt
     4381 atcataataa atactatctt ttacctttcc attaagatga tagctgagat ataagccttg
     4441 ttttttacca ttttgatgaa aatatttttc attgagattg ccattttcgt accaagagta
     4501 gcatggacct gtctcttgat tttcttcctt tgctagtttt ttatttggat aatagtattt
     4561 ccaaatgcca atttttttgt catctttata aaaaccttct aaagcttttt gtccgttacc
     4621 gtgatatagc tcataggggc catctttttt gccattttta taagtaatct tagatattaa
     4681 tgtcccatga gtagtgtaag ttttatgctc accatccatc tggccattta ctatatgcca
     4741 ttcttctatt ttattaccta atgaatcata aataatgaca ggaccagtat cggagctaat
     4801 aattttccac aatttaccat tttcaaaata atattcccat tgaccaatca ttttaccttc
     4861 agagaaatat tgtctacttt gaatattccc attttcataa taaataatcc attccccctc
     4921 aggtttgcga ttttttaatt gtcctgtcat tttcaaagta tcaggatatt tataataagc
     4981 ttttatagga ccattatctt ttgcttcaac aatatagatt tcgccattag gataatattg
     5041 tttccaggtg cctatttcta aaccattact atcatattca ccctccatag agagctgccc
     5101 attatgaaaa taaaaatacc atttacctat acgagtacta tctggcaaag agataatgcc
     5161 tttttctttt atttgtccgt ctggataagt ttttataatt tttgtttttt tctgagcaaa
     5221 aataggattt attattaaaa tcagtagtaa taatatacaa tatgaacgca ttttttatta
     5281 caaatttaaa aataaaattt taatataaaa aatccattaa aaaaactatt ttattaataa
     5341 tttccctcac cagtatcaat attttcatag atttttttca aaaatctatc tttagttgtt
     5401 gtagttgcta tgtaatatga atatgctttt tctttgccgc tggatggcaa gattcttgat
     5461 ttagaatctg tattaattat taattcatta aattcttttc gagaagacaa gccttgcatc
     5521 agtccattat tataagtaaa aaagtaccaa acatttggta atggttctat gtagatagtt
     5581 aatctatcac cactacgcct tagagataac tctattattc cattcacata tttataaact
     5641 tgtttgccat tagcaacaga aatgccaatg tatccttctg aaacataagt tttagtctct
     5701 ggtatatatc taaaagtgac atcattaaaa acaaaagttt tcattaattc tttaggcatc
     5761 tttcgaatat ttccatatag atttagatct tttattacat tcatggcgtc agtactatct
     5821 atgatttctt cgataaatga aaggaataat tcatcttgaa tgtttagtcc tggcaagtca
     5881 gatcctaata tatcagttgc caacatatct agagctttat cattaaagaa aaaatctaaa
     5941 tttatatttc cattaataat agtagaatct gagtagatat aatggtctac cctaccataa
     6001 aaatccatag ctactctacc cagattagac ccaagactca gcttaccctc acctctcact
     6061 ttgcaattat gtgtatttaa acttagatag ttacctttca ttgtgagtcc atcaaatttt
     6121 tctttggacg ttatctgata ttcatttgat ggctcatcat acttcaaaaa accgtgagaa
     6181 gacaccagtt ctatatcacc accatataat ttttttgtta aaaaagctgg atatacacct
     6241 gcattttctt tactcgaaaa atataaacca gcaaaaatat tctctcctga ggtgttttta
     6301 ggttgagcag gtataggtat cattatatca gtaggatcta tcatttgatt aaatttaacc
     6361 cattgtttag ttagggtatc gcaattatgt gagattttga taccgccatc gaaggataaa
     6421 aattcctgat cggcatacag atgtacatcg ccagtataaa ggaaatatgg gctaagagta
     6481 aaattgacac tatcgggtac attaccatta gctgttgttt gcgattcatt gactacgaaa
     6541 attttattaa aataaattaa ttgagaatta ttatttttat ctacatattc gtagtgtcca
     6601 tttccagaat agcttttttt accatagatc catattttac aattatgcaa aatatgatat
     6661 ttggtttcta gattagctaa aacttttgaa ttttctaaag gtttcatttc agcatgcggt
     6721 aatatatata ctataccatt atcaggaaaa atagcagcat cagctacttg aatatatggt
     6781 acttcgtaag ctgtaattat tttatctctt atattaaaat tagctttacc agaaataaat
     6841 gataatgaat cttgagcggg atgtgtagaa ataaatatag agccttctaa atccatgtct
     6901 acaaggtctc tagatgaaat atgagtataa tctacatctt ttctctttat agtataaaag
     6961 tctaattctt ctttatccat ataccaatca aatttatcca taaagcaaat atattcatta
     7021 gcaggaaatc gtatcataga attttctcca gttgtttcga aagagccttt tcttttatca
     7081 aagtctacat ggcccttata aatttgagtt tctagtgcaa attcatttaa atccaaagtt
     7141 tttagtacaa aatcacaagt atctgtatca aaaactcgat ttttcaatgt aaatttttta
     7201 ctattcataa tagcatttaa aaattctatt ttaccattaa caaagagtcc caaaggacta
     7261 taatctaata taccattaat ttttatattt tctttataag ctatagcggg ggtctctatt
     7321 gtattaatag acataaaatc ttgtttagga taccatatta ttttagcatt ttctacattt
     7381 acaggtgggt attgaatacc agtgctacgt tcagtaataa cgtagctatc tactattcct
     7441 ttagcagaat cagggaaaaa tagtaaattt ttactaatag ctgtagaggt gatatatttc
     7501 aatgtgccat ttccatataa tccttgatta gacatagtga tttcattaac aaaagtccct
     7561 tttccaccat aagctggtag cccatcagat gtagttttat gaaaaatacc tagagaataa
     7621 tcatccatta ttattagtgg ttcgtatata tcaggaaaaa taccaccaga agctaggtaa
     7681 ccatcaaatt taagtgaatc agtttgaaat ttatctaaat tttctaatct ataaggattt
     7741 atgtgataat aaaaatcatc tcttaggtat acatcttgat aaatgcatga tttgtcataa
     7801 tatacataag atattttttt actatcaaaa ataggatact ctggcatata aattttacca
     7861 cttttattac taggatggtc tattaatatc tctccactaa ggtcttctat cacatttttt
     7921 actcgcacta atgggtattc accatattta tctggtactc tgcttcttac ataaaaattc
     7981 atagaatcta cgtatggcat atttatttta aaatcttcat atgaaaaata atattctcta
     8041 ccaaaataat taaatctacc tgcacttatt acaccattga aagtaaaatc tctatttttt
     8101 ttcacaataa ccatttgtgc ctcaggataa acaacgacat tctgtgaatc actcagaaat
     8161 actctagata caccttctaa ttttaaatca taattaatta aacttagcct agcattatct
     8221 tttgattcta ctacagaatt aaattgtatc acatcgaaat cttttttacc aacattagct
     8281 tcgatgtagt cataaatttt atcttttgca taaaccatat ctgtcgttgg attgtaatca
     8341 acgaagcctt gtagtgcaag agatataatc tgctgacgaa taatacgaat atctattttg
     8401 cgaaatttag caaaatctaa aaccgaaaaa tatttttttc ctgtttgatc tataaactct
     8461 ttaatatatt gcaagggatt tttctcatct attccttgta gcttcaaata tcgagactta
     8521 gagaaataat tgttagattc aaacaatgca tttcctaatg acccaccttt catcatagac
     8581 attctgattt cgggactacc tatagtccag taaagagctt cgaaatacat atctatattg
     8641 tgatatgtat tataaaaagg cataggatgt agacgttcat tatctctaat gcacattatt
     8701 tcttttgtgg aatcttgata tttaaaaatt aatgacatat gatatatcga atcttcattt
     8761 agatatatta ctacagaggc atcttgacta tctatactat tttttctaat agaaaaacta
     8821 ttagattgtg attttataaa taatttatta tcataaaata atctaatagt agcaggacta
     8881 atgctatcac catgcccaat gatgctagcg ccttttactg taaaaccacc ttcataatca
     8941 atatttttaa atatattatt aatgtaaaaa ttggtttgat atgaatagaa ctgcggataa
     9001 atagattgct ctccagtttt atctgcaaag atacgttcta ttaagaatcc tgtaattggt
     9061 tcatctccaa aataatcttt atgataaaaa tttacactgt cagctctgta atctcgagat
     9121 tttagtgaaa tggtataatt acttaatata gcataaactc cttctggatc tagtaaagct
     9181 ctttgccagg taattttacc acctttgccc tcccatttgt agttattcat ataatatgtg
     9241 ccactggtat gataaatcac agaactatca ccgtatgcat aacattttag agtagtatta
     9301 gggaaaatga ttttgggttc tttgtcatat tcgaaatgaa aatcattaga ggtgctcatc
     9361 caagtagtag tgggcgatga gtacaagatg gcatttagaa ataagctatc gctctgagag
     9421 agaaattgta tgattttttg tgctctttct ttttctacaa tatttttata tgtttgcgac
     9481 caagcagtat atgcactttg ccatttgcca cttttttgaa tatttattaa tgaatgcaaa
     9541 aatgataaaa aatgctctga gccaagcctt ttttttaaca ttgcattaga taattctatg
     9601 atagattgtt tctccaaatc agtgatagag ctccagaacg ttgagaattc accaagtaaa
     9661 aatgcatcta tttcttttaa agtactttta ctgatatttc tatcttgacg aaacaattgc
     9721 tctaaccctg atatgtatgt cgtcggatct tgagtaaatt gtgttatttg ggcgtgtaga
     9781 ttaatactaa ataatataaa aactgtaata aatataaatg aaattttttt cataaataat
     9841 taattaacta attaatttaa caaaaataat aaaaaagaat tgaaataaca gttttgatta
     9901 ataaaaaaat agcggcaaca ttaatttaca ctaataacct ttgtgaatat tttaataaat
     9961 ttagaattga ggattatttt taatataata ttagcaatta attaattgca aatattaata
    10021 gatgattcaa aaataatcaa cacttagtat ttttacatta agtattcttt gtgtctttct
    10081 ggcaaattac gatctatgat gtaaagggta attttaatag ctccacgctc agaatatcca
    10141 tatttatcgc acattacact tatcatatga cgaatatctt cttggatttt attatcataa
    10201 ttgccaaatt tgtcagtgaa atagtctttt agtgcaaacc taaaagattg attttttagg
    10261 tatggatcga gacttgcttt cttaatattt tctttatata gatcaaataa atcttgaatc
    10321 aactgtgtgt gttgccattt tttttgcttt acctctatct cttgagcaag ggtttcggtg
    10381 ataaatctat ttaacaaatt atttctgttt ttatctctat cgactatctc aggaaataaa
    10441 cgattttcta tagattctaa gaattcatta gttactatat atgttttatc tgtaaaagga
    10501 tttgtaactt tagctcctat atcatagctg agagctaaaa gataatgaat gatgtcttct
    10561 aatattttat tttcattata agtaaaaagg gcgtctttta gctcttgtac tatatttatt
    10621 tcataaattt tgagaagcga gtttataaaa tttggtggaa taagtttttt aacactatcg
    10681 ggttgatcgt tgaaaaaatt aataatatct agcatatcta tctcttcatc tttttcttga
    10741 tatgtagata gcaatttatt gcacaatgct atagattgtc taccagaaat acctttaaat
    10801 ccttcatcta aggcttcatt taaaacagct aaacgtatat ttcttttaaa accctgtata
    10861 tcttcttctt cgagccaacg cggtatcctg ccatgataaa gttccatttt taataataaa
    10921 agatcaggat ctatatattt ttcatactta cttctatgct ttagccatct tttcaatgta
    10981 tctgattctg tatttagtct agtgcaaata atgattttag caaaattttg caaaacacgt
    11041 ggcaagaatt ttttttgaat attactacca aatttatttt cgtaaatacg tacctctgtt
    11101 tctgcatcta aaacgtaagg aatcaacaca aatctcactc tatcttgaaa agatttaata
    11161 tctataaaag tttttttgtc ttctggattt actaatccca aaaacaaggt atttattcgc
    11221 tcctcgatat gctccacttt gtgtacccca tcacttataa taccatgcaa agagagtaat
    11281 ctagtaatat tattatcttt tatatccatt atagcataaa taccattatt agtatatgct
    11341 agatatgaat atttacattc tatctcctcg gtagacaaca aatttcgtaa ttcattttgc
    11401 aaattatcat taatgtaaat atgttctatt aggttatctg caggattcca tacagatata
    11461 cctttgccaa gttggcgatt aaaatctatt cgtttagcat agatcatatc atatacttgc
    11521 aatggatctt ctagtatatc tattagtgaa ttataaatag atttgcaaat agagcacggt
    11581 tcgcttctaa aaagccattc ataaattttt gattctataa tatcttcata tactttagga
    11641 tctttgatca gggtttttaa aaaatctttt cttagctctt taggaattat taaaaaagga
    11701 ttgtcattat gagggcatga atatgagatt atttttccat tttctatttt attgtttata
    11761 tctttagtgt cgattttcca aaatatttta tagacagtac cctcgtcagt gagcgtatat
    11821 tcttgcattt tttgtaacag attatttaaa aaagtagatt taccactacc aggagggcct
    11881 tcgaaaagat aaatttgatt tacattgata cctttattga tttcttcagc catttgcata
    11941 aatctattag caaacaatct atcactaaaa aaaggtactt ctacgttctc tactaataat
    12001 ttagataaat cataaaaact aaatccaaca ttttcgctat cattttcata ctcatcagta
    12061 ccttctggta cataataatg caccatatca tgaaatagtt gaaatatatt tctcaaaact
    12121 tgcttggggt tggtagataa taggaataaa aagtcattaa aattaatagg atatctctgc
    12181 tctttttctc ttatttgctt agtaagtcct aataaatatt ctagttcatt agcggtttta
    12241 tcataaggta ttaatggatt ttcatcttta tgtggcataa taatattatt tttgtaaatt
    12301 taataacttt tttataagca attctttttt atatagaaaa atattttatc tcaaggtaat
    12361 ttatttaatt tttttttaat tttgttaaaa aaaatttatg aaacccaaat tgattatcat
    12421 tttattctgt ttagttttaa tgtttaattc ttgtgctaaa actggtaata aaaaaaagga
    12481 tgaaagaaaa aacaataatg ttcaaaatct tagtattttg atacctatat acatggaaca
    12541 acctattaaa gagtggacat attttttaca agcttcacat cctaatgtta aatttgaaat
    12601 aatctgtatg aatgaacaat attttagtag tgatactact tttagagatt ttgatttaat
    12661 atttacccct agttttatta gaggaaacaa atataaatat tctattataa gccaagaagc
    12721 tatagtctgt gtagtgtctt tcgaaaatcc atttctacaa tccatcatta atagaggcat
    12781 tagtcctaat gatttaaaaa agatattttc taatcctaat tcttattatt ggaaagattt
    12841 tttcaaaact gataaaaaat taaatgataa aattactatt atcttaccag atgatagata
    12901 tacattgata aatcaattat ttgattggct agatatagat aaacctacat cagtaactat
    12961 gaaatctaca gattctattt tagatttatt aaaaattcaa aatgacatta tgactttttt
    13021 accctcatca cttgtttata atattaatac tcaatacaga aaagattttt tgtacgtagt
    13081 accaatagat ttgaataatg ataactggat cagtgatgac gagtatttat atgataatat
    13141 tataatgata aaaaaagctg ttttagacaa taaaatagat tcattattgg ttttgcaata
    13201 taaattaata tattctatag ataattctcg taaaaattta atagaagaca tagtaaataa
    13261 taagcataat ttaaataaaa atatcttcga acaatatggt tatttcgtta attaaacaaa
    13321 ttaaatttgg cttttagtca ataataatag gcaaagaaga attggagcaa ttgataaatt
    13381 gataggggca attcacgatt tgc
//
LOCUS       DAOPRN010000050        13169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_3985_length_13169_cov_143.365869, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000050 DAOPRN010000000
VERSION     DAOPRN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3985_length_13169_cov_143.365869"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1201
                     /gene="era"
                     /locus_tag="PKI65_06260"
     CDS             320..1201
                     /gene="era"
                     /locus_tag="PKI65_06260"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOB78079.1"
                     /translation="MHKAGFVSILGYPNVGKSTLVNTIIGEQLMPVSPKAQTTRQRVL
                     GIYNDDDTQIIFSDLPGWIIKPQHELHKSMLQDIKSAFDDSDILIYLYDLSSNKENKE
                     LLDIFSDLSIPKIITLNKADLVNEEKILITENFLKEKFQNIDIIPISALNGYNVDLLI
                     KTIKNYLPIHPPYYDKDILSDRYLRYFASEIIREQIYYLYKEEIPYETEVIINNFKED
                     QDPVYIEAEVWVMRESQKKIIIGKNGEMIKQLGINARLKLEKLLDKHVFLQLYVKVKP
                     NWRNNKNILKSLGYNVK"
     gene            complement(1501..1929)
                     /gene="rpiB"
                     /locus_tag="PKI65_06265"
     CDS             complement(1501..1929)
                     /gene="rpiB"
                     /locus_tag="PKI65_06265"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOB78080.1"
                     /translation="MMKVAIGCDHAGFEMKEYLRNKLETKCEVLDCGTYSLEPSDYPD
                     FAHKVAELIVNKEVDYGILMCGSGNGVNIVANKHKGIRSAYCWIPELAVLARKHNDAN
                     VIALPARFIAKELALEIVKKFLNTKFEGGRHKKRVEKIEI"
     gene            2318..3235
                     /gene="nadA"
                     /locus_tag="PKI65_06270"
     CDS             2318..3235
                     /gene="nadA"
                     /locus_tag="PKI65_06270"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOB78081.1"
                     /translation="MKKNIKELKKENNAIILAHYYQMPEIQDIADYVGDSYALAKYAQ
                     NTDADWIILAGVKFMAETAKVLNPNKRVFVPDINAGCSLVDAAPVDKFKKWIDSFENP
                     FVVTYINSSIEVKMLSDVIVTSSNAVKIVSKIPKDKTILFAPDKNLGDYVMKKTGVNM
                     QIWDGNCYVHNQLDAKRIINLKNIYPDATIIAHPECKTDVLDLCDFVGSTNSLLNYVK
                     NTNYNVYIVATETGILHQMKKAKPNADFILVTLDDNSKHNDCKYMKLNTLEKIYDNLI
                     NQTNEILLSEAQIERAKISILRMIEFAEN"
     gene            3425..3961
                     /locus_tag="PKI65_06275"
     CDS             3425..3961
                     /locus_tag="PKI65_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOB78082.1"
                     /translation="MYSKLPIFIGITGPESTGKTWLAERLSHYFYIDYIPEYAREYLL
                     NLKRPYTYDDVIKIAEKQWNLINNTLNTDQKIVIYDTEMLVIYVWLLYKYGKCPKWIE
                     EAWRNQKIDLYLLCYPDIEWDYDPLRENPNDLIELFDIYETNLKKENKSYYIVKENRE
                     QRFINVLNYLKINFPHIF"
     gene            4028..5842
                     /gene="mrdA"
                     /locus_tag="PKI65_06280"
     CDS             4028..5842
                     /gene="mrdA"
                     /locus_tag="PKI65_06280"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOB78083.1"
                     /translation="MRSYENRKWFFIYFFIFVFIILTIRVFYIQIINPTYKTLAEQNT
                     LRYVYEYPQRGTIYDAEGRLLAWWRPVYNLSIIPFELEPFDTIKLANLLELTQEQLTS
                     RIEKAKKMGGYQPITIYEDLSDFVVSYLYEHESEYKGILLEKRFMRDYPLKIGAHFLG
                     YISEVNPKKLEEDAYYRMGDYIGVTGLEEFYEKELRGRKGVKVYVADVHNRIVGRYKN
                     GLLDSSLVNGKNLYTSIDADLQSYGEDLMNNKRGAIVAINPKTGEIITLVSAPSYDPN
                     LLTGRNRSMNYLMLSNDVKNHPLYNRATMTRYPPGSTFKIVNGLAALQENFINEKTFF
                     SCYGGYRIASHTIGCHGHPSPTNIISAIQYSCNTYFCQVFKVFVDNKYFSKSSIGYER
                     WRYYVNSLGFGVELGIDLPNEYGGFVPTAEYYNRLKGTRNWRADGVISVAIGQGEIGA
                     TPLQLANLATIIANRGWYIPPHIVRAVGHPDSLNKRYNTKIVVPINQNYFDLIIEGME
                     KAVTAGTATIAMLPEIRVAAKTGTAQDPPRKSHSVFIAFAPVDNPKIAIGVLVENAGW
                     GASYAGPIASLMIEKYLTDTVKRIDLENRMKVAEILNK"
     gene            complement(5855..6349)
                     /locus_tag="PKI65_06285"
     CDS             complement(5855..6349)
                     /locus_tag="PKI65_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOB78084.1"
                     /translation="MQIGILSDTHGFLPEDVYNFFKNCDEIWHAGDIGDIKIITNLNK
                     IANTIAVYGNIDNNSIRLKLPDEIFFERNNLKIYLKHTIGYPNKYQNNALSKLIKYKP
                     DIVVTGHSHILQIIYDKKYKWLFINPGAAGIYGPHNKITLVKLTLENAKIINAKIFEK
                     EKTI"
     gene            6606..7952
                     /gene="gdhA"
                     /locus_tag="PKI65_06290"
     CDS             6606..7952
                     /gene="gdhA"
                     /locus_tag="PKI65_06290"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOB78085.1"
                     /translation="MTDYKKIVDDFMVDVIAKNPGENEFHQAVREITESLVPFIEEHP
                     KYKEAKILERMVEPERVIIFRVPWQDDKGEIHVNRGYRVQMNSAIGPYKGGIRFHPSV
                     NLSIMKFLAFEQVFKNSLTTLPMGGGKGGSDFDPRGRSDAEVMRFCQSFMNELYRYIG
                     PDTDVPAGDIGVGAREIGYFFGQYKRLKNEFTGVLTGKGIQYGGSVIRPEATGFGVVY
                     FTQEILKTMGMDIKGKKVAISGFGNVAWGVVRKVDELGGKVITLSGPDGYIYDPDGVS
                     GEKIDFMLEMRRSGRDKVQDYADKFNVEFYPGKRPWSVPCDIAIPCATQNEVDLEDAK
                     QIVKNGVKCYVEAANMPTTLEAMKYIQENNIPFGPAKAANAGGVAVSGLEMTQNAMHL
                     SWTPEEVDKKLHEIMINIHNTCLKYGKEGDKVNYVKGANIGGFVKVADAMIAQGIV"
     gene            8219..9217
                     /locus_tag="PKI65_06295"
     CDS             8219..9217
                     /locus_tag="PKI65_06295"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78086.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEIVTSHRQKRGIYSLSTVKKKTSEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNITYVN"
     gene            9681..10283
                     /locus_tag="PKI65_06300"
     CDS             9681..10283
                     /locus_tag="PKI65_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78087.1"
                     /translation="MSNWQYLKKKTFWAHIGLMLVAFIVFIFLVKFFLKIYTKHGREI
                     ELADFTYKTIDEIKNEKVKLIVIDSIFNEDLPPLTIVNQYPEPGMKVKPGRKVYVTVV
                     SKSYEKVPLPDLTDLTLRAATLQLQAYGFEIGKIEKIPSIGQTVIAIKQNGREIPWGS
                     LLKKGSTVNIVIGSGFSPSVDTLQFINEIDYDIYSDDELM"
     gene            10346..11299
                     /locus_tag="PKI65_06305"
     CDS             10346..11299
                     /locus_tag="PKI65_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78088.1"
                     /translation="MNLVKAFNIILLIFLLFLFNSCKEKTEILTSKIIYDVYISPVEI
                     EQPHVNYLSPKKRQEVLKFVTKAFKTNKVTDSIGNIITIDNLSKKIYELDTNVNAIDN
                     ASKLLEKFILDQWDVIRFEESWEYNKNTGQIFKAVKNLSFMKGEKDSFMMPTMSKHIF
                     SIDVANIKMKKPDLDKSYVIYDVCIIPLVESTSPYYHNISLSSRQKYFTDLFNAVRNN
                     KAIVLDYFYEKIPRDKISDLFVIKGIEEFTNKEISIPISIEEIGRIKFIEQWYWDTSN
                     LTLNKYVFGVNPGLQVRKEDDLIGFSPLFWAIFNKKIINDL"
     gene            11289..12200
                     /locus_tag="PKI65_06310"
     CDS             11289..12200
                     /locus_tag="PKI65_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOB78089.1"
                     /translation="MIYKNNLEHCTLCPRNCNAKRYEKKGICRASEKIEVSSIIIHKG
                     EEPVLSGKHGILNVFFANCNLRCIYCQNYQISLYNQYSTEYSVDKLVNKIKQLLPPEI
                     YHIGWVTPTHYANYIPEITEKLKRWRDDLKFVYNTSAYDKPNMIKELKDYVDVYLPDF
                     KYSLPEVAYKYSKARDYTEFAIKSITEMIYQKGTTLQIDDNGLAISGVIIRHLVLPEN
                     INNSLKVLDILSDNFGINLWISLMSQYTPIKKLDLPLELQRPLKRKEYKIVVNYMQEL
                     GFHHGWVQDLESIDYLFPDFSNKSNPF"
     gene            12301..13053
                     /locus_tag="PKI65_06315"
     CDS             12301..13053
                     /locus_tag="PKI65_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOB78090.1"
                     /translation="MIKNKKVVIVFPAYNAEKTLEQTYNELPHDIIDEIVIVDDCSQD
                     NTVEIAKRLGIKHIIRHEKNKGYGANQKTCYDKSLELMGDIIIMVHPDYQYTPLLIEP
                     MAYIIANGLYPVVFGSRILGRGALKGGMPLYKYISNRILTFFENIMLGQKLTEYHTGY
                     RAYSREVLKTINYHANSDDFIFDNQIIAQIFYAGFSIAEISCPTKYFPEASSISFKRS
                     VNYGLGVITTSFQYRFQKWKLARYEYLEPITK"
BASE COUNT         5029 a         1635 c         2001 g         4504 t
ORIGIN      
        1 gtactgacta tttcagggct aaagcccata acttttcttt gttattcctc gacctaaagg
       61 ttgtagttaa tatacaacgt tttgatcctc gaccttctct ttgttttatc ataaaacatt
      121 ttgtctaatc ataaaaacct ttatcatcat cctcaactac taaactcctc cactactaaa
      181 ctttatccat caatctaatc atccgaaaat ctgtggctca gactatgtgc gatgatacgt
      241 ttatcatcgg cgtaattata taatacaaaa ttatcaaaat aataatttta tgtatatttg
      301 tattgtaata ttaaatttta tgcataaagc aggttttgta agtatacttg gctatcccaa
      361 tgtaggtaaa tctacattgg taaatacaat aattggcgaa caattaatgc cagtatcacc
      421 aaaggcacaa actacacgtc aaagagtttt aggtatttat aatgacgacg atacacaaat
      481 tattttttca gatttaccag gttggataat aaaaccacaa catgaattgc ataaatctat
      541 gttacaagat attaaatctg ctttcgatga ttctgatatt ctaatttatt tatatgattt
      601 atcaagtaat aaagaaaata aagaactctt ggatatattt tctgatttgt ctatacctaa
      661 aataataact ctcaataaag ctgacttggt aaatgaagaa aaaattttaa taacagaaaa
      721 ttttttaaaa gaaaaatttc aaaatataga catcatacct atatctgctc ttaatggtta
      781 taatgtagat ttattgatta aaactataaa aaattactta cctatacatc ctccttatta
      841 tgataaagat atactatctg atagatatct tagatatttt gcatcagaaa ttataagaga
      901 acaaatatat tatttatata aagaagagat accttatgag acagaggtta ttataaataa
      961 tttcaaagaa gatcaagacc cagtgtatat agaagctgaa gtatgggtaa tgagagaatc
     1021 tcaaaaaaaa attattatag gtaaaaatgg agaaatgata aagcaattag gtataaatgc
     1081 tagattaaaa ttagaaaaat tattagataa acatgtattt ttgcaattat atgtaaaagt
     1141 aaaacctaat tggcgtaata ataaaaatat attaaaaagt ttaggttata atgttaaata
     1201 atttggttct ataatgtttt gagtgggtaa aaacattata gattgttaca aaaaataact
     1261 atcgtaattt agattaattt ttttaccaca aagagcacca agttattaca caaagtccac
     1321 aaagtgctat ttatagccct tttaatttca tccttctctg tgttctttgt gcattctttg
     1381 agaactttgt ggttaatatt tgactgtcca ataattaata aaccaatatt aaaaatttac
     1441 tctttttaat atttaaacat ataaaacgtt atagaacctt ataaatatac atttattatt
     1501 ttagatttct attttttcta ctcttttttt atgccttcca ccctcaaatt ttgtatttaa
     1561 aaatttttta actatttcta aagctaattc ttttgctata aatctggcag gtaaagctat
     1621 cacattggca tcattatgtt tacgagctaa tacagctaat tcaggtatcc aacaatatgc
     1681 agatctaata cctttatgct tattagctac tatatttact ccatttccag atccacacat
     1741 taatatacca taatctacct ctttattcac tattaactca gctactttgt gtgcaaaatc
     1801 tggataatct gatggttcta aactataagt accacaatct aagacttcac acttagtttc
     1861 taatttgttt cttaagtatt ctttcatttc aaaacccgcg tgatcacaac caattgctac
     1921 tttcataata tattattttt tatatgtaaa taaatttaaa ataacttgta aataacttgt
     1981 aataaattat ttttttattt acacttaatt tttatattac atatatattt gtttattact
     2041 aatttatttt ttgtaaaaat ataaacattt ttattaacag atttttctgt taataagtaa
     2101 aaaaataatt tacattgaca aaattaataa aaaatgataa ttgtcaataa atttttttta
     2161 attaataaaa atctataatt tagttttaat aaaatgttaa taacatgtag aaaactttat
     2221 aaaaataatt ataattattt ttgaaaaaaa gttatttaca aatttacatt attattatta
     2281 ttaatattat taaaattaaa aaaaaggaaa atattatatg aagaaaaata ttaaagaatt
     2341 aaaaaaagaa aataatgcta taattttggc acattattat caaatgccag agatacaaga
     2401 tatagctgat tatgtaggtg atagctatgc attagctaaa tatgctcaaa acacagatgc
     2461 agattggatt attttagctg gtgttaaatt tatggctgaa actgctaaag tattaaatcc
     2521 taataaacgt gtttttgtac cagatattaa tgctggttgt tcgctggtag atgcagcacc
     2581 agtagataaa tttaaaaaat ggatagatag tttcgaaaat ccttttgtag ttacttatat
     2641 taactcttct atagaagtta aaatgttatc tgatgtaatc gtaacaagta gtaatgctgt
     2701 gaaaatagtt agtaaaatac ctaaagacaa gacgatactt ttcgctcctg acaaaaatct
     2761 aggtgattat gtaatgaaaa aaacaggtgt aaatatgcaa atatgggatg gtaattgcta
     2821 tgttcataat cagttagatg caaaacgtat tataaattta aaaaatattt accccgatgc
     2881 tactatcata gctcatccag aatgtaaaac tgatgtttta gatttatgcg atttcgtagg
     2941 tagtactaat tctctattga attatgttaa aaatactaat tataatgtat atattgtagc
     3001 tactgaaact ggaatattac atcaaatgaa aaaagcaaaa ccaaatgctg attttatact
     3061 tgttaccctt gatgataatt caaagcataa tgattgtaag tatatgaaat taaatacttt
     3121 agaaaaaatt tatgataatt tgattaatca aactaatgaa attttgttat cagaagcaca
     3181 aatagaaaga gctaaaattt caattttgag aatgatagaa tttgctgaga attaaaaaaa
     3241 atcttattaa ttgcttctat aacgtttatt gtgggtaatc aaattttata ttgactgtcc
     3301 aataattaat aaaccaatta ttaacaaatt tatttttgta aaattatttt tttaaatttt
     3361 tacccatata aaacgttata gaaccttttt ttttaaaaaa aaaactacta tttttgtaat
     3421 aaaattgtat agtaaattac caatattcat agggataaca ggaccagaat ctacaggaaa
     3481 aacatggtta gcagagcgat tatctcatta cttttatata gattatattc cagaatatgc
     3541 acgtgagtat ttattaaact taaaaagacc ttatacctat gatgatgtca ttaaaatagc
     3601 cgaaaagcaa tggaatttaa taaataatac cttgaatact gaccaaaaaa tagtaatata
     3661 tgatacagaa atgcttgtga tatatgtttg gcttttatat aagtatggaa aatgtccaaa
     3721 atggatagaa gaagcctggc gtaaccaaaa gatagattta tacttacttt gttatccaga
     3781 tatagagtgg gattatgatc cattacgtga aaatcctaat gatctcatag agctgtttga
     3841 tatttatgag actaatctaa aaaaagaaaa taaatcctat tacattgtaa aagaaaatcg
     3901 agaacagcgt tttattaatg ttttaaacta tttaaaaata aattttccac atatttttta
     3961 aattattctg tattttcttt taattttgta aaaattatta aatttattgt ggcaaaaaaa
     4021 gtaattaatg cgttcatacg agaataggaa gtggtttttt atttattttt ttatatttgt
     4081 ttttataatt ttaactatca gagtttttta tattcaaata ataaatccaa cttataaaac
     4141 tttagccgaa caaaacacat taagatacgt atatgagtat cctcaacgtg gtactatata
     4201 tgacgctgaa ggtcgtttat tagcatggtg gagacctgtt tataatttat ctattatccc
     4261 ttttgagtta gagccttttg atactattaa attagctaat ttactagaat taacacaaga
     4321 acaattgaca tctagaatag aaaaagctaa aaaaatggga ggttatcaac ctataactat
     4381 ttatgaagat ttatctgatt ttgtagttag ttatttatat gaacatgaat ctgaatataa
     4441 aggtatttta ttagaaaaac gttttatgcg tgattaccct ctcaaaatcg gtgctcattt
     4501 tttagggtat atatcagagg taaatcctaa aaaattagaa gaagatgcat actacagaat
     4561 gggagattat atcggagtaa caggtttaga agaattttat gaaaaagaat tgaggggcag
     4621 aaaaggtgta aaagtatatg tggctgatgt acataataga atagttggta gatacaaaaa
     4681 tggtttatta gattctagtt tagtaaatgg aaaaaatttg tatacaagca tagatgcaga
     4741 tttacaatca tacggtgaag atttaatgaa taataaaaga ggtgctattg ttgctattaa
     4801 tcctaaaact ggcgagatta ttacacttgt atcagcacct agttatgatc ctaatctatt
     4861 aacaggacgt aataggagta tgaattacct aatgcttagc aatgatgtaa aaaatcatcc
     4921 tttgtataat agagctacta tgactagata cccgcccggt agtacattta aaattgtaaa
     4981 tggtttagct gctttacaag aaaattttat taatgaaaaa acttttttta gttgttatgg
     5041 tggatataga atagcttctc atactatagg atgtcatggg catccttctc ctacaaatat
     5101 tattagcgcc atacaatatt cttgcaatac ttatttttgt caagttttta aagtatttgt
     5161 tgataataaa tattttagta aatctagtat tggttatgaa cgttggcgtt attatgtgaa
     5221 ttcattaggc tttggagtag agctaggtat agatttacct aatgaatatg gtggatttgt
     5281 gcctacggca gaatattata atagattaaa aggtactcgc aactggcgcg ctgatggtgt
     5341 gatttcagta gctattggcc aaggtgaaat aggggctaca cccttacaat tagcaaatct
     5401 agctactata attgctaata gaggatggta tataccacca catatagtga gagcagtagg
     5461 gcatccagat agtttaaata aaagatataa tactaaaata gttgttccta taaatcaaaa
     5521 ttattttgat ttaatcattg aaggtatgga aaaagctgtt actgctggta cggcaactat
     5581 agctatgttg ccagaaatac gtgtagcagc taaaacagga actgcacagg atccgcctag
     5641 aaagagtcat agtgttttta tagcttttgc gccggtagac aatcctaaaa tagctattgg
     5701 tgttttagta gaaaatgcag gatggggggc ttcttatgca ggacctatcg ctagcctaat
     5761 gatagaaaaa tatttaactg atacagttaa aagaatagat ttagaaaata gaatgaaagt
     5821 agctgaaatc ttaaataaat aaaataacga gtctttatat tgttttttct ttttcaaaaa
     5881 ttttagcatt tattatttta gcattttcta atgtcaattt tactaaagta atcttattat
     5941 gtggcccata aatacctgct gctccaggat ttataaataa ccatttatat tttttatcat
     6001 agataatctg taagatatgt gaatgaccag ttacgactat atctggttta tatttaatca
     6061 atttagataa agcattattt tgatatttgt taggatatcc tattgtatgt tttaaataaa
     6121 tttttaaatt gtttcgttca aaaaatattt cgtcaggtaa ttttaacctt atactattat
     6181 tatcaatatt accataaacc gcaattgtat tcgctatttt attcaaattt gttattattt
     6241 taatatcgcc aatatcacct gcatgccata tttcatcaca atttttgaaa aaattataaa
     6301 cgtcttctgg taaaaaacca tgagtatcac tcaaaatacc aatttgcata aaaatcaatt
     6361 ttttattaaa taaaacaaag atataaaaat aaattaatat aaatcaatat aataattgat
     6421 atatatcatt ataaaatatg gtaaaaatat tttaaaaaat aataattttt taaattcata
     6481 cataatatca gtatttcaga atgttttata agaagtttaa aatcaaaaaa acataatttt
     6541 tattaataaa taaaatataa tatgataaaa aattataact ttgcagaaac aaaaatattt
     6601 aagctatgac agattataag aaaatagtag acgattttat ggttgatgta atagccaaga
     6661 atccaggaga aaatgaattt catcaagcag ttagggaaat aacagaatcc ctagttcctt
     6721 ttattgaaga acatcctaaa tacaaagaag caaaaatatt agaacgaatg gtagaaccag
     6781 aacgtgtaat aatatttaga gtaccttggc aagatgataa gggagaaata catgtaaata
     6841 gaggttatcg tgttcagatg aattcagcta taggtccata taagggtggt atacgttttc
     6901 atccaagtgt aaaccttagt ataatgaaat ttttagcatt tgaacaagta tttaagaatt
     6961 ctctaactac actaccaatg ggtggaggta aagggggttc agattttgat ccacgtggta
     7021 ggtcagatgc tgaagtaatg cgcttttgtc aatcatttat gaatgaatta tataggtata
     7081 ttggaccaga tactgatgta ccagctggag atataggagt aggtgctaga gaaattggtt
     7141 acttttttgg acaatataaa cgtttaaaaa atgaatttac tggtgtattg actggtaaag
     7201 gaatccaata tggtggaagt gtaatacgtc cagaagctac tggtttcggt gttgtttatt
     7261 tcacacaaga aattttaaaa actatgggta tggatataaa aggtaaaaaa gttgccatat
     7321 ctggttttgg taatgtggct tggggtgtag ttagaaaagt tgacgagtta ggtggtaagg
     7381 ttatcacttt atcaggtcct gatggatata tttatgaccc agatggcgtg agtggtgaaa
     7441 aaatagattt tatgctagaa atgagacgct ctggcagaga taaagtgcaa gattatgctg
     7501 ataaatttaa tgtagaattt tatcctggta aaagaccatg gagtgtgcct tgtgatatag
     7561 ctataccttg tgctactcaa aatgaagtag atttggaaga tgctaaacaa atagttaaaa
     7621 atggtgttaa atgttatgta gaagctgcta atatgcctac tactttagaa gctatgaaat
     7681 atattcaaga aaataatatt ccttttggcc cagctaaagc tgcaaatgca ggcggcgtag
     7741 ctgtttctgg tttagagatg acgcaaaatg ctatgcatct cagctggact ccagaagaag
     7801 ttgataaaaa attacatgaa attatgatta atattcacaa tacttgcctt aaatatggta
     7861 aagaaggtga taaagttaat tacgtaaaag gtgctaatat aggggggttt gttaaagtag
     7921 cagacgctat gatagctcaa ggaatcgtat aattaaaaaa ttaaattaat tttttaaaag
     7981 gctttatgta taaatgcata aagccttttt tttatattta agtatatttt taaagtatta
     8041 ccacgttgtt gatttttttt cattgtaaag tgcgaaacaa tgaaaaaaaa tatcaagata
     8101 taatattgtt tggtgatttg gaaatcaggt aaaaataaac atttattcgt aataaatgtt
     8161 taagtttttc aagtttatat gttaaataat ttttatattt ttataaaaat tttaatttat
     8221 gaaaaaaata gtagaaaaca ccagtaaata ccttgctatg agtaaggaag agaataaaga
     8281 attcttagac aacctttatt cattaatgaa aaaattagaa gccgatgtag tcgattatca
     8341 aggcctaaaa atactcagcg ccccgccact atgcccagac tgtcgtagca atcacatcat
     8401 taaaaatggc ttacaaaatg ggatgcaaaa ctatcgttgc aaagattgcg gcagacaatt
     8461 tagagttacc actggtactt ttgtttacag attgcaaaag aaagagaaaa tgcttgatta
     8521 catcagatgt atggtagcag gcaaaagtct cagagcttgt gctaaagaag taggcatcag
     8581 cttaccaaca agcttcgctt ggcgacacaa gatattggca gctttacaaa gtttcgaaga
     8641 taatataaat tttttcggag tagtagagat ggatgaacta ttgatggatt attctgaaaa
     8701 aggcagaata tacaaaaatg ccgaagagct gaagcgagct aggaagcaaa agccaaagaa
     8761 agtagccgta ttggctgcta cagacagagc tggcaactta ttattcaaaa aatttgatgg
     8821 caaaaagcta agttcagaac agatagagca atttctgaag gctaaaatgc caaaggatgg
     8881 agtcatgtgt agcagtaata aaaaagcatt caaaaaggtt agaagaaatg taatcaagca
     8941 taaagaaata gtaactagcc ataggcaaaa gcgaggtata tatagcctga gtactgtcaa
     9001 gaaaaaaact agtgaattca caaattggat atatttcaaa tttagaggag tagctaccaa
     9061 atacctgcaa aactacatta tgtggtatgt agtgatgggt aaatacctca caggcaagac
     9121 tgtaagcaat actgggcgaa tgctcaacct cgcatcttcg gacaggtggg catggtatcg
     9181 gtatagagag ttagtgaata taacatatgt taattgaaac atatgttaga tttccaaatt
     9241 gattaatttt caagtttcca attctcctca tccaacatat cttaaggtta taatttgtgt
     9301 aataaaattt tagattgtta caaaaaaatc attatttttt aattatttag gttatttttt
     9361 accacaaaga gctatttgta gcccttttaa ttccatactt ttctgtgttt tttgtgcatt
     9421 ctttgagaac tttgtggtta atatataact gttaactgtc caataataaa taagccaata
     9481 ttaaaaatca actcttttta atttttatac atataaaacg ttatagaact attttttgat
     9541 atacttttct aactttattt taattaattt cttaataata aatcataact aattatatac
     9601 agtgtgaaac aaaaattaca agaaattatt atatattata tttttttcaa ttaactttgc
     9661 ataatacaaa atgattataa atgtctaact ggcaatattt aaagaaaaaa acattttggg
     9721 ctcatatagg gctaatgtta gttgctttta ttgtttttat cttccttgtt aaattttttc
     9781 taaaaatata tactaaacat ggcagagaaa tagaattagc tgattttaca tataaaacaa
     9841 tagatgagat caaaaatgaa aaagtgaaat taatagtgat agatagtata tttaatgaag
     9901 atctgcctcc attaacaata gtaaatcaat atcccgaacc tggtatgaaa gttaaacctg
     9961 gccgcaaggt atatgtgaca gtagtaagta aatcatatga gaaagttcct ttacctgatt
    10021 taactgattt aacattgcgt gctgctactt tgcaattaca agcttatggt tttgaaattg
    10081 gtaaaattga aaagattccc agtatagggc aaactgttat tgctataaaa caaaatggta
    10141 gggaaatacc atggggctca ctattaaaaa aaggtagtac tgtaaacata gttataggta
    10201 gtggttttag cccatcggta gatacattac aatttatcaa tgaaatagac tatgatattt
    10261 attcagatga tgaattgatg taatattatg tattatttag attaataaat atttttttta
    10321 ttaactatta aatataatgg aaaatatgaa tttagttaaa gcatttaata tcattttatt
    10381 aattttttta ttatttcttt tcaattcttg taaagaaaaa acggaaatat taacttctaa
    10441 aattatttat gatgtttata taagtccagt agagatagaa caaccacatg taaattatct
    10501 cagtccaaaa aaacgacaag aagtattaaa atttgtaaca aaagctttca aaaccaataa
    10561 agtaactgat agcattggca atattattac tatcgataat ttatctaaaa aaatatatga
    10621 attagacaca aatgtaaatg caattgataa tgcatcaaaa ttgctcgaaa aattcatttt
    10681 agatcaatgg gacgtaataa gatttgaaga atcctgggaa tataataaaa atactggtca
    10741 aatttttaaa gccgtaaaaa acctttcatt tatgaaagga gaaaaggact catttatgat
    10801 gcctacgatg agtaagcata ttttcagtat cgatgttgca aatataaaaa tgaaaaagcc
    10861 tgatttagat aaatcttatg tgatttatga tgtttgcatt atccctctgg tagagagtac
    10921 atctccttac tatcataata tttcacttag tagtcgtcag aaatatttca cagatttatt
    10981 caatgctgtt agaaataata aagctatagt tttagattat ttctatgaaa aaattcctcg
    11041 cgataagatc tcagatttat ttgtaataaa aggaatcgaa gaatttacaa ataaagaaat
    11101 ttccatacct atatcaatag aagagatcgg caggattaaa tttatcgaac aatggtattg
    11161 ggatacatca aacttaactt taaacaaata tgtatttggt gtaaatccag gcttacaagt
    11221 aagaaaagaa gatgatttaa ttggattctc cccattattc tgggctattt ttaataaaaa
    11281 aattattaat gatttataaa aataatttag aacattgtac tttgtgtcca agaaattgta
    11341 atgccaaaag gtatgaaaag aagggtatat gtagagcttc tgaaaaaata gaggtttcat
    11401 ctatcataat acataaggga gaagagccgg tactaagtgg aaaacatgga atattaaatg
    11461 ttttttttgc caattgtaat ctcagatgca tttattgtca aaattatcaa attagtttat
    11521 ataatcaata ttcaacagaa tattcggtag ataagctagt gaataagata aaacaactac
    11581 tccccccaga gatatatcat attggatggg ttacacctac acattatgct aactatatac
    11641 cagaaataac agaaaaatta aaaagatggc gagatgatct aaaatttgtc tataatacct
    11701 ctgcatatga caaaccaaat atgataaagg aattaaaaga ttatgtagac gtttatttgc
    11761 cagatttcaa gtattcgctt ccagaggttg cctataaata ttctaaagca agagattaca
    11821 ctgaatttgc tataaaatct attactgaaa tgatatacca aaaaggcact acacttcaga
    11881 tagatgataa tgggttggca atatcaggtg ttatcattcg ccatttagtt ttacctgaaa
    11941 acataaataa ttctctaaaa gtactagata ttttgtctga taattttggt atcaatcttt
    12001 ggatatctct aatgtctcaa tatacaccta ttaaaaaatt agatttacca ttagaattgc
    12061 aaagaccttt aaaaagaaaa gaatataaaa tagtagtaaa ctatatgcaa gaattaggat
    12121 ttcatcacgg atgggtgcaa gatttagaat ctattgatta tttattccct gatttttcta
    12181 acaaatctaa tcctttttaa agaaaaatac tatttagaca taaagtattt agctgtaaac
    12241 tgaattattt tacttattta aataaaaaat taataatttt atcttttaaa agtaaaaaat
    12301 atgataaaaa acaaaaaagt tgttatagtt tttcctgcat acaatgctga gaaaacatta
    12361 gaacaaacat ataacgaatt accacatgat attatagatg aaatagtaat agtagatgat
    12421 tgtagtcaag ataatacagt agaaatagca aaaagattag gcataaaaca tattatcaga
    12481 catgagaaaa acaaaggata tggtgctaat caaaagacct gttatgacaa atcattagaa
    12541 ttaatgggtg atattattat tatggtacat cccgactacc aatacactcc tttattaata
    12601 gaaccaatgg catatataat agcaaatggt ttatatccag ttgtgtttgg gtcacgtatt
    12661 ttggggcgtg gtgccttaaa aggaggcatg ccactatata aatacatatc aaatcgaatt
    12721 ttaacttttt ttgaaaatat tatgttgggt caaaagctca ctgaatatca tactggttat
    12781 cgtgcatatt cacgagaggt acttaaaact attaattacc acgccaatag tgatgatttt
    12841 atttttgata atcaaataat agcacaaatt ttttatgcgg gtttctctat agctgagatt
    12901 agctgcccta caaaatattt tccagaagct tccagtatat cttttaagcg aagtgtcaac
    12961 tatggtttgg gagtaataac cacttcattt caatacaggt ttcaaaaatg gaaattagct
    13021 agatatgagt atttagaacc aataacaaaa taataaatta ttaattacga aaatgatgcg
    13081 taacttatca aaaatctcat ctgttaaaat ttgcacagga ttaatatcag aaaatttatt
    13141 aaatttgcaa aaaataaaaa attatgaaa
//
LOCUS       DAOPRN010000051        13051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_4030_length_13051_cov_110.459911, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000051 DAOPRN010000000
VERSION     DAOPRN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13051
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4030_length_13051_cov_110.459911"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..893)
                     /gene="radC"
                     /locus_tag="PKI65_06320"
     CDS             complement(210..893)
                     /gene="radC"
                     /locus_tag="PKI65_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOB78091.1"
                     /translation="MPKKSKITIKELPKIERPREKLMKYGPDKLSDEELLAIILRSGT
                     KDENVIDLATNILKKFPKDKLPYTTFDELKDYHGIGPAKACEIIACFELGKRFLKDKQ
                     TRIFLSPKQVWESLKDIRESKKEHFVIFYLDTKNQEIIHEIISIGTLNASLVHPREVF
                     EPAVKNLAAQIILAHNHPSGDYQPSEDDIKITQKLVEAGNILGIEVIDHIIVTKKDFF
                     SFRDKGLIQ"
     gene            complement(957..2819)
                     /locus_tag="PKI65_06325"
     CDS             complement(957..2819)
                     /locus_tag="PKI65_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB78092.1"
                     /translation="MKKIINFILLLIFYTTLISQEFIVPLNYNPHLINNHSKNESKNT
                     TINTLNLPIKIDVADYFGYPPSEYFTDSTAFCNNEFGFYPFTIGVITLDALDKNGQIY
                     EHAQYTPFPADTLTSCYIRLDSFFVGIPHSLHISDSLYFSFAFQPQGLSDAPETNDSL
                     ILEFYDNENDMWEKVWACQGTSLQNFIDSFGKPWKVIMIPIKDDKFLTSQFRFRFRNL
                     ASIANNQIPNWNSNGDIWNIDYIYINKNRHWHDTLPVDIAFRGQIYSLLKKYVSMPWK
                     QFLANADEEMITNFSIPYTNFSDQTCNITEYIEIIDLANVGAPYQPSLSALNVPPYTD
                     TVFTRFTLPYTFNTISDSTAEFKITFAINTATIPDTLNQNDTIKIYQRFYNYYAYDNG
                     FPTAGYCLSGYDAKLAYKFELNVPDTIRSIEIFFNSLSSFIDYNYFRLIIWDDINGKP
                     GNIIYQEEKLLPQHYEDLGKFYNFILNEPVALPSGVFYVGFMQYQSSSLNIGFDGNND
                     AHNNIFYNVDGIWRNSQYSGALMIRPIVGNSPYPYIYVPEHLNNKPKINIYPNPAYTN
                     STIYIDAPFIDYWELYDVNGKFITKSKDHSFNINQPGFYIIKINGNEFFKLIIY"
     gene            complement(2904..3593)
                     /locus_tag="PKI65_06330"
     CDS             complement(2904..3593)
                     /locus_tag="PKI65_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOB78093.1"
                     /translation="MKKTSIIVLLFLFLFRAHAQNESMMNLVTFDMRPVHFGFILGFN
                     SLSFSAKLKPNDTILTAVPIPQSGFEIGITADLNLASHLNLRFIPGLSFGSRKVKFTV
                     RQPDGTIETITKSAESTFLDFPLYLKYKTDRLTNFRAYALLGAQYSLDLASQSKKKED
                     KLDLLKINPHNINLLIGGGGEFYLRYFKFGIEIRMTYGLNDVITRRGTVFDKNMDYLK
                     SKIFWIVFTFE"
     gene            3759..4508
                     /locus_tag="PKI65_06335"
     CDS             3759..4508
                     /locus_tag="PKI65_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOB78094.1"
                     /translation="MKKIYYLIFLIFIFSCTTYQIKDQQSTSKTITIDTTIKNDDNVD
                     KWIMKYKTSLDSLMDLVIGFNKISMIKNTPESNLGNFVCDLILDFINRELKDTLEGLP
                     CIVVLNNGGFRSSLPEGQISIRDIYSVMPFDNTLTLLKISGENMIKLAQQIIEKGGMP
                     IGGIEIIATATTLKQTKINGNDISKNQQYWVATSNFIANGGDNLIGLMNPIKRIDTPY
                     LIRDIIIEHYKLLTSQNKAAYAEKDGRIRYE"
     gene            4474..5451
                     /locus_tag="PKI65_06340"
     CDS             4474..5451
                     /locus_tag="PKI65_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HOB78095.1"
                     /translation="MQKKMGGLDMNNGRRDFLKSVLGIGAGLLLPKIAKPNTANNLHE
                     ELGLKKITILHTNDTHSQIEPLPKNHYRYPGQGGYSRRAYLINQIKRDNDKVFIFDSG
                     DLYQGTPYFNIYKGKLEIEYLNKMYYTAITIGNHEFDLGLNVLGENLLQANFDIISSN
                     YVFTNDPILSKIVKPYKIYNIDKLKIGVFGLGIEPTGLIDKNLWGKTIYKDPMETAAK
                     MALLLKKEYKCNLVICLSHLGYKGSEGRIGDIEIAKSSRNIDIILGGHSHTLLSKPVY
                     ISNREKKPVIITQNAYAGVQMTQLDCYFNSFGSFLFAEEHNKKIFYKNV"
     gene            5536..7017
                     /gene="dnaA"
                     /locus_tag="PKI65_06345"
     CDS             5536..7017
                     /gene="dnaA"
                     /locus_tag="PKI65_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOB78096.1"
                     /translation="MKEIEKKISQNKMTKLDILWNDILEFIKENINYQNFKTWFLPLK
                     PLSFENNTLIIEVPSSFYYEYIEAHFIDVLKVALKNFIGPNAKLCYQVVIAKETDKSS
                     TLQWPSNDKVATSNPDIHIPLKTEDIKSLKEKKSNSLPNPFLIPGIRTYSIDSNLIET
                     LDFSNFVEGECNRLARAVGLSIANNPGLTSFNPFFVYSTTGLGKTHLVNAIGLETKKN
                     FSNKIILYMNTAEFLNQFIKVVKSDNISDFIQIYQSVDLLIMDDIHHLANKPKTQEVF
                     FQIFNILHQQDKQIILTSDKPPIELEGIEERLLNRFKWGLTAELTKPDYDTRIQIIHK
                     KLQRDGINLTSEIIEYIAKKVKDSIRELEGVLIAIIAHSTFVKKDLDINDVQNLIDQY
                     IKCNQQTITYDMIKHVICDYFAITLDQLESSTRKQPIVMARHMAMHFASKYSKLTLTE
                     IGRLTGNRKHSSVAYAIKNVNNLLEVDKNFRMQYEEIKKKIIK"
     gene            7311..9821
                     /locus_tag="PKI65_06350"
     CDS             7311..9821
                     /locus_tag="PKI65_06350"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOB78097.1"
                     /translation="MQKRAYLLFILLLVDFFVLLGQNLDTLLQNNLVLDSIIIQNGDT
                     VKTQIAKSSQAIDKKVDYQCKDSLVMEMKDKKITLYENAKVVYDNLTLTAGIIKIDFD
                     NNLVYAYGIYKDSIYSERPNFNQGNQSFLADSLVYNIKTGKGLVYSVFSKQGDGYLHG
                     KYVKRENDSVILVKTGGFTTCELEHPHYIIRFDKAKVVTDKEIITGPVWLEVEDITMP
                     IGLPLGFFPTRTKRQSGILLPTVGESANRGFYLENIGYYFGFSDYVDLALRGDIYTRG
                     SWAIKAETNYKKRYKYEGNLQANFAHNKFGEKGFSNYEVYNDFFIRWYHKQDPKAHPT
                     NRFSANVQLGSSNYTKFNPSSANDYLSNTFTSSIAYSTQLFNLFNLNINARHTQSNTT
                     HRVDVSLPDLSLSSNTIYPFRPKKLTKPLKWYQNLNLRYQLQAKNNLSRPDSTFFNNL
                     SFKDFNIGFQHNLPISLNINFFKYLQWSNQFNTNWRWYFKSIDKNWNPIDSNMVIDTL
                     YKFRMPLDISYTTSLSTRLYGFYSFGKFPLRVIRHVITPSISYTWRPDFSVKPWNYYN
                     VCHTPNGEEWYSPFEGAIFGYPPRGKSSAISFAIANNIEAKIRQRTDTGEMLKKIVLI
                     ENLSLSTSYNFAADSFQLSPLIISARTKLFKNFELRYALGLDPYHYDTTGRRIDKFQF
                     DIDKKLFNTQNQEWNLSGNLILQYPNIKRGKTSTSEHNTKKIYPNFRVTIGLNLRHYS
                     SYIPHTNIDFTKSIVANGNIEFTPNWSVSFMTGYDFDLKDFTYTSINIHRDLHCWEII
                     LNWIPTGFRKSYNFTIRIKSPMLQDVKLTKKTDWRDYY"
     gene            10141..11421
                     /locus_tag="PKI65_06355"
     CDS             10141..11421
                     /locus_tag="PKI65_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOB78098.1"
                     /translation="MKLSIIQDADLKDKLVLVRVDHNVVEKGLIHDPYRIDATISTLY
                     YIVSHGGKLILMTHVGRPKNKKDGTINISNDTSVEPIVEYLHNKLNLNFKIPEFYVNE
                     PYGIQDIDTSVNNLIKELKLGKIDGIYLPNTRWYRGEEDKGEWQERLANQLAGLADIY
                     VNDAFGSWQPHVSTYTITKYLPSYAGLLLQKEINNLDKIFSPIKPMIAIIAGAKFDTK
                     INSLKALIKDVDYLMLGGVILNAFLAAKYKISIKGIDDDNLSMAKEFVDFVQTNYPNK
                     LIEPRYIVESDTMEGKIEGHYKIIDVKSLKAGETLNYVLDVAPESFNDPSIIEKFKSA
                     KTFFVNAVMGYAPHFNDGTIALNELINSNNDAIKLYAGGDTLQELKRLLPGIYMKALD
                     DSKFYMFTGGGAVLTAIEQNSPYGMEPVKALLID"
     gene            11526..12560
                     /locus_tag="PKI65_06360"
     CDS             11526..12560
                     /locus_tag="PKI65_06360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOB78099.1"
                     /translation="MLNIKKILISRTDNIGDLVLTLPLAGFLKQNFPDIQIAYLVKDY
                     TKPIADSYKYIDEVYSIDKINEETKEKERIKLINSIDADVIIFVYPDREVMSLVAKSN
                     ISRRIATSHRTYSWRYASNLVNFSRKNSYLHEAQLNFLLLKPLQIDYLPSINELLQWY
                     GKDKFYIKPIPKDLINDFAISPYRPIIIHPGSMGSAPNLSPQHFLDLTNKLVELGENI
                     YITGNEKEGLLWQPYFDKLNVKTLFGSLNLYELISIISKAKAVIASSTGPLHIAAALD
                     TNAIGLYVPIGTYAPHRWSPIGKNAKVLTPAYHKISCKSKCISPSNCTCINEIKIEKI
                     INEIYDIETE"
     gene            complement(12816..>13051)
                     /locus_tag="PKI65_06365"
     CDS             complement(12816..>13051)
                     /locus_tag="PKI65_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78100.1"
                     /translation="VKKKASEFTNWIYFKFRGVATKYLQNYIMWYVVMGKYLTGKTVS
                     NTGRMLNLASSDRWAWYRYRELVNITYVNFNIY"
BASE COUNT         4945 a         1704 c         1900 g         4502 t
ORIGIN      
        1 agattatcta ggaatctacg tttctgctct ttgggtagag cttgataatc taataaatta
       61 aaattatcca taattatttt tttgtaaaat taaataaaaa ttatttgatg gtagtataaa
      121 atacgtaaag taaaacaaaa aatattattt ctccaaccct catacaaagc ctaatatttc
      181 caaaaaaatt aatttttagt attagctaat tattgtatta atcctttatc tctaaaacta
      241 aaaaaatctt ttttggttac gattatatga tctattactt cgatacctag tatattacct
      301 gcctcaacta gtttttgagt aattttaata tcatcttcag atggttgata atcgccagaa
      361 ggatggttat gagccaaaat tatttgtgca gctaaatttt taacagctgg ttcgaaaacc
      421 tctcgaggat gaacaagact agcatttaat gtacctatag atatgatttc atgtataatt
      481 tcttgatttt tggtatcaag ataaaaaatt acgaaatgct cttttttact ttctctaata
      541 tcttttagcg attcccatac ttgttttgga gagagaaaaa ttctagtttg tttatctttt
      601 aaaaatcttt ttcccaattc aaagcaagca attatttcac atgctttagc aggtccaatc
      661 ccatgataat ctttcaattc gtcgaaagta gtataaggca atttatcttt ggggaatttt
      721 tttaatatat tagtagctaa atctattaca ttttcgtctt tagttccaga cctcagtata
      781 atagctaaaa gttcttcatc tgagagttta tcaggaccat atttcattaa tttttctcta
      841 ggtctttcta tttttggtag ttcctttata gtaattttac tttttttagg catataaaat
      901 caatatattg gcaaatataa taaaataata ttcatttaaa ccaaaaaaag tctcaatcaa
      961 taaattatta atttgaaaaa ttcattacca ttaatcttaa taatataaaa accaggttga
     1021 tttatattaa aactatgatc tttacttttt gtaataaatt taccattaac gtcatagagt
     1081 tcccaataat ctataaaagg agcatctata tatatggtag agttagtata agctggatta
     1141 ggataaatat tgattttagg tttattattt aaatgttcgg gtacatatat ataaggataa
     1201 gggctattac ccactatagg tctaatcatc aatgcaccag aatattgaga atttcgccaa
     1261 atgccatcta cattataaaa aatattatta tgagcatcat tattgccatc aaagcctatg
     1321 tttaatgaac tactttggta ttgcataaat ccaacataaa aaacacccga tggtaatgct
     1381 acaggctcat ttagaatgaa attataaaat ttgcctaaat cctcataatg ttggggcaaa
     1441 agtttttctt cttgataaat tatattgcca ggcttgccat taatgtcatc ccaaattatt
     1501 aatctaaaat aattatagtc tataaaacta gataaggaat taaaaaaaat ttctattgat
     1561 ctgatagtat ctggtacatt gagttcgaat ttataagcta atttagcatc atatccagat
     1621 agacaatatc ctgcggtagg aaatccatta tcataagcat aataattata aaaacgttga
     1681 tatattttga tagtatcgtt ttgatttaaa gtatcgggta tagtagctgt attaatagca
     1741 aaagtaattt taaactctgc tgtactatca gaaatagtat taaaagtata tggcaaagta
     1801 aatctagtaa aaacagtatc agtatatgga ggcacattaa gagcacttaa agatggttga
     1861 tagggggctc ccacattagc taaatcaata atttcaatat actcagttat attgcatgtt
     1921 tgatcagaaa aattagtata gggtatagaa aaattagtaa tcatttcctc atcggcattt
     1981 gctaaaaatt gcttccaagg catagaaaca tattttttta acaaagaata tatttgtcct
     2041 ctaaaagcta tatctactgg taaggtatca tgccaatgtc tatttttgtt aatatatata
     2101 tagtctatat tccatatatc accattacta ttccaattag gtatctgatt atttgctata
     2161 ctggctaaat tacgaaacct aaaacgaaat tgtgaagtca gaaacttatc atctttaata
     2221 ggtatcatga tgactttcca aggttttccg aaactgtcaa taaagttttg aagacttgtt
     2281 ccctgacaag cccatacttt ttcccacata tcattttcat tatcataaaa ctctaagatt
     2341 aatgaatcat ttgtttcagg agcatcactc agcccttgag gttgaaaagc aaaactaaaa
     2401 tataatgaat cgctaatatg caaactatga ggtataccta caaaaaagct atctaatcta
     2461 atgtagcaag aggttaatgt atctgcagga aaaggggtgt attgagcatg ttcgtatatt
     2521 tgaccatttt tatctaatgc atctaatgtt attacaccaa tagtaaaagg ataaaatcca
     2581 aattcattat tacaaaaagc tgtgctatct gtaaaatatt cactaggagg gtagccaaag
     2641 taatcagcta catctatttt gattggtaga ttaagtgtat taatagttgt gtttttactt
     2701 tcgtttttag aatgattatt tattaaatga ggattatagt taagaggcac gataaattcc
     2761 tgacttatca aggttgtata aaaaatcaga agtaaaataa aatttattat ctttttcatt
     2821 taaaatttat tactattttt ttttaacaaa attataaaac tattttttaa aatattacca
     2881 taatataaat tcaataaaaa atattattca aaagtaaaaa ctatccaaaa aatcttagat
     2941 tttagataat ccatattttt atcaaagacg gtgcctcgcc tagtgataac atcatttaaa
     3001 ccataagtca ttctaatttc aataccaaat ttgaaataac gtaaataaaa ttctcctcca
     3061 ccaccgatta gtaaatttat gttatgaggg ttgattttaa gaaggtctaa tttatcttcc
     3121 ttttttttag attgtgaagc gagatctaaa ctatattgag cacctagtaa tgcataagct
     3181 ctaaaattgg tcaatctatc agttttatat ttcaaataaa gggggaaatc taaaaaagta
     3241 gattctgcag atttagtaat agtttcgata gtaccgtcag gttgtctcac agtaaactta
     3301 acctttcgag aaccgaaaga taaaccaggt ataaagcgta aatttaaatg agatgctaag
     3361 tttaaatcgg cagtaatacc tatctcaaat ccgctctgtg gaataggaac ggctgttaat
     3421 atagtatcat taggttttag ttttgcacta aaagacaaac tattaaaacc caagataaaa
     3481 ccaaaatgta caggacgcat atcaaaggta actaaattca tcatgctctc attctgcgca
     3541 tgtgctctaa agagaaataa aaaaagtaag acaataatac ttgttttttt cataaatatt
     3601 aaaacgcaaa attaaaatta atattgtaaa tactgaaatt attcaaaaat agagaatata
     3661 aatatttaag gaaaatcata aaataaaata cctaatttaa tatattgaaa tattttattt
     3721 tcttaaaaaa aaacattatt tttgaaaaaa tgtattctat gaaaaaaatc tattatttaa
     3781 tatttctaat ttttatattt tcttgtacaa cttatcagat aaaggatcaa cagtctacat
     3841 caaagactat aaccatagat actacgatta aaaatgatga taatgtagat aaatggatca
     3901 tgaagtacaa aactagttta gatagtttga tggatttagt aataggattt aataaaatat
     3961 caatgataaa aaacactccc gaaagtaatc ttggtaattt tgtttgtgat cttattttag
     4021 attttattaa tagagaatta aaagacacat tagaaggttt accatgtata gtagttttga
     4081 ataatggagg ttttcgttct tcattgcctg aaggtcaaat cagtattaga gatatttatt
     4141 cagtgatgcc ttttgataat acacttacat tactaaagat atctggcgaa aatatgataa
     4201 agctagcaca gcaaataata gaaaaaggtg gtatgccaat aggtggaata gaaataatag
     4261 ctactgctac tacactaaaa caaactaaaa taaatggtaa tgatatatca aaaaatcaac
     4321 aatattgggt tgctacttca aatttcatag ctaatggtgg tgataattta ataggattaa
     4381 tgaatccaat aaaacggatc gatactccat atttgattcg tgatataata atagaacatt
     4441 ataaattatt aacttcacaa aacaaagctg cttatgcaga aaaagatggg aggattagat
     4501 atgaataatg gtagaagaga tttcctaaaa tccgttttag gtataggagc tggattatta
     4561 ttacctaaaa tagctaaacc taacactgct aataatttac atgaggaatt gggacttaaa
     4621 aaaataacaa ttttacacac aaatgatact catagtcaaa tagaaccctt acctaaaaat
     4681 cattatagat atcctggtca gggtggttat agtagaaggg catatttgat aaatcaaata
     4741 aagcgtgata atgataaagt atttatattt gattcaggag acctctatca aggcacgcct
     4801 tattttaaca tatataaagg taaattagaa attgagtatt tgaataaaat gtactataca
     4861 gctattacga ttggtaatca cgaatttgat ttgggattaa atgtattagg tgaaaattta
     4921 ttgcaagcca attttgatat aataagctca aattatgtat ttacaaatga tccaatattg
     4981 tcaaaaatag taaaaccata taaaatttat aatattgata agttaaaaat aggagtattc
     5041 ggtttaggaa tagaacctac ggggttaata gataaaaatt tatggggaaa aaccatttat
     5101 aaggatccaa tggagacagc tgccaagatg gcattattat taaaaaaaga atataaatgt
     5161 aatttagtaa tatgtttatc tcatttagga tataaaggaa gcgaaggtag aataggtgat
     5221 atagaaatag cgaaatcgag tagaaatatt gatataatct taggaggtca tagtcataca
     5281 ttgctttcta aacctgtata tatatcaaac agagaaaaaa aacctgtaat aataacacaa
     5341 aatgcttatg ctggcgtgca aatgactcaa ttagactgct atttcaactc ttttggttcg
     5401 tttttgttcg cagaagagca taataaaaaa atattttata aaaatgttta aaaaattttt
     5461 taatttaaaa tattttgtgc atttttgtta tttgaaaaaa ataatcaaaa tgtagtttat
     5521 actaaataat taaaaatgaa ggaaatagaa aaaaaaatct ctcaaaataa aatgacaaaa
     5581 ctagatatac tttggaatga tatattagaa tttataaagg aaaatattaa ttatcagaat
     5641 ttcaaaacat ggtttttgcc gttaaaacca ctttcttttg agaataatac acttattatt
     5701 gaggttcctt catcctttta ttatgaatac atagaggcgc attttattga tgtattaaag
     5761 gttgcattga agaattttat aggtcctaat gctaaattat gctatcaagt agttatagct
     5821 aaagaaacag ataaatcatc tacattacaa tggccatcaa atgataaagt agctactagt
     5881 aatcctgata ttcatatacc attaaagact gaagatataa aatcattgaa ggaaaaaaaa
     5941 tctaattctc tgccaaatcc ttttttgata cctggcatta gaacatattc aatagatagt
     6001 aatctaatag aaaccttgga ttttagtaat tttgtcgagg gtgaatgtaa tagattagct
     6061 cgtgcagtag gtctgagtat agcaaataat ccggggctaa cttcttttaa tccttttttt
     6121 gtgtatagta ctactggact tggaaaaact catttagtta atgctatagg cttagaaact
     6181 aaaaagaatt tttctaacaa aattattctt tatatgaata ctgctgagtt tttaaatcaa
     6241 tttataaaag ttgtaaaatc agataatatt tcagatttta tccaaattta tcaatctgtt
     6301 gacctattaa taatggatga tattcatcat ttagctaata aacctaaaac tcaagaggta
     6361 ttttttcaaa tattcaatat tttgcatcaa caagataaac aaattatatt gacctcagat
     6421 aaaccaccta tagaactaga aggcatagaa gagagattgc tgaatcgatt taaatgggga
     6481 ttaactgctg aattaacaaa accagattat gatacacgca tccaaataat acataaaaaa
     6541 ctacagagag atggtatcaa tctaactagt gaaatcatag agtatattgc taaaaaagta
     6601 aaagatagta ttagggagct cgaaggagta ttgatagcta taattgcaca ttctactttt
     6661 gtaaaaaaag atctagatat taatgatgta caaaatctaa tagatcaata cattaaatgt
     6721 aatcaacaaa ccattaccta tgatatgata aaacatgtaa tatgtgatta ttttgctatt
     6781 acattagacc aattagaatc atctactcga aaacaaccaa tagtaatggc aagacatatg
     6841 gcgatgcatt ttgcttctaa atattctaaa cttacattaa cagaaatagg tagactcaca
     6901 ggtaatcgta aacactcttc tgtggcttat gctatcaaaa atgtaaataa tttattagaa
     6961 gtagataaaa atttcagaat gcaatacgaa gaaataaaaa agaaaataat taaataatta
     7021 aaacctattt attctcgagt aattttacta ctaatattaa tatacatttg gatttagtat
     7081 ttgtgagttt cagtttatta gttaaaaaaa atgtataatt tttttgtatt tattaaatat
     7141 tctattacaa gaaaaaaata aaattaatat aataaaaaag aatggctgtt tcataatttt
     7201 atcctttttt caataatttt aaattaaaaa tttttctatt ctataatcat ttctgaattt
     7261 tgcagttaat ttgtaaaata attataattt aaaatatttg attaattaaa gtgcaaaaga
     7321 gagcatattt actatttata ctattattag ttgatttttt tgttttgcta gggcaaaatt
     7381 tagatacttt attacaaaat aatctagtgc tggatagcat aataattcaa aatggagata
     7441 ctgtaaaaac acagatagcc aaaagtagtc aagctattga taaaaaagta gattatcaat
     7501 gtaaagacag cttagtgatg gagatgaaag ataaaaagat aacattatac gaaaatgcaa
     7561 aagtagttta tgacaatctt actctcactg caggtattat aaaaattgat tttgacaata
     7621 atcttgtgta cgcttatggt atctacaaag attccatcta ttctgagcga cctaatttca
     7681 atcaaggcaa tcaaagtttt ttagcagatt cattagttta taacataaaa actggcaaag
     7741 gattagttta ttctgttttt tcgaaacaag gtgatggcta tttacatggc aaatatgtaa
     7801 agagagaaaa tgacagtgtt attttagtaa aaactggtgg attcactact tgtgaattag
     7861 agcaccctca ttatataatt agatttgata aagctaaagt cgtaactgat aaagagataa
     7921 taactggtcc tgtgtggtta gaagtggaag atatcactat gccaataggt ttgcccttag
     7981 gttttttccc aactagaact aaaaggcaat caggcatctt gttacctact gtaggcgaga
     8041 gtgctaatag gggtttttat ttagaaaata ttggttatta ttttggtttt tctgattacg
     8101 tagatttagc attaagaggt gatatttata ccagagggag ctgggctata aaagctgaga
     8161 ctaactacaa aaaaagatat aaatatgaag gaaatttgca agcaaatttc gctcataata
     8221 aatttggaga aaaaggtttt agtaactatg aagtttataa tgattttttc atacgttggt
     8281 accataagca agatcctaaa gcccatccta caaatagatt ttctgctaat gtacagttag
     8341 gttcttctaa ttatactaaa tttaatccat catctgctaa tgattatttg tctaatactt
     8401 tcacttcttc tatcgcatat tcgacacaat tatttaattt attcaatcta aacatcaatg
     8461 cgcgtcacac tcaaagtaat actactcaca gggtagacgt ttctttacca gatttgagct
     8521 tatcatctaa tactatttat ccatttagac ctaaaaaatt aactaaacct ttaaaatggt
     8581 atcaaaattt gaatttaaga tatcaattac aggcaaaaaa caatctatct cgccccgata
     8641 gtacgttttt taataatctt agtttcaaag actttaatat tggttttcag cataatttac
     8701 cgatatcttt aaatataaat ttttttaaat atctacaatg gtctaatcaa tttaatacaa
     8761 attggcgttg gtattttaaa agcattgata aaaattggaa tcctatagat agcaatatgg
     8821 taatagatac tttgtataaa tttagaatgc cactagatat ttcatatact acatcattgt
     8881 ctaccagatt atatggattt tattcatttg gaaaatttcc attgcgtgta atcagacatg
     8941 tcattacacc atctattagc tatacatgga gaccagattt tagtgttaaa ccttggaatt
     9001 actataatgt ttgtcataca cctaatggtg aggagtggta cagtcctttc gaaggagcta
     9061 tattcgggta tccaccacgt ggaaaatcat cagccattag ctttgctata gctaataata
     9121 tagaggcaaa aataagacaa agaactgata ctggggaaat gttaaaaaaa attgttttaa
     9181 tagaaaatct ttcactcagt actagttata attttgcagc tgatagtttt caattgtctc
     9241 cattgataat ttctgcacgc acaaaattgt ttaaaaattt tgaacttcgc tatgctttag
     9301 gattagaccc ttatcattat gatactactg gtaggcgaat cgataaattt caatttgata
     9361 tcgataaaaa acttttcaat actcaaaatc aagaatggaa tctgagtggc aatttaattt
     9421 tgcaatatcc taatattaaa agagggaaaa cttctacatc agagcataat actaaaaaaa
     9481 tttatccaaa ttttcgtgta actataggat taaatctaag acattatagt tcgtacatac
     9541 cacatactaa tatagacttt actaaatcaa ttgtcgctaa tgggaatatt gaatttacac
     9601 ctaattggag tgtatctttt atgactggct atgattttga tctaaaagat tttacttata
     9661 cttctattaa tattcataga gatctccatt gttgggaaat aattttaaat tggataccta
     9721 cgggttttcg taaatcatat aatttcacta ttcgcataaa gtccccaatg ctacaagatg
     9781 ttaaattaac taagaaaact gattggcgtg attattatta atatattgtt ctacaataag
     9841 tatgcaagtt taatagattt atagcttatt agttaattat ttcaatgaat gacgttgaga
     9901 aaacactact tatcacatta attgattttg tgatttgttt ttatcaattc tcagttttag
     9961 agatttgcat agcataaaac tttttaaaat atattaaatt aatttttttt ttaattttca
    10021 tatattcatt actagataga ttatataaaa aaagcctcat aaatttgagt aaatttatga
    10081 aattaaatgt tttgttaata attttaaaaa ttaataaatt tgcataaaaa tcataaaatt
    10141 atgaaattat caataataca agatgctgat ttaaaagaca aattagtttt agtgagagta
    10201 gatcataacg tagtcgaaaa agggttgata catgatccat atcgtataga cgctactata
    10261 agcacattat attatattgt atcgcatggg ggtaagttaa tactgatgac acatgtagga
    10321 cgtcctaaaa acaaaaaaga tggcacaata aacataagca atgatactag cgtagagcct
    10381 atagtggagt acttacacaa caaattaaat cttaatttta aaattccaga attctatgtg
    10441 aatgaaccct atggtataca ggatatagat acatcagtta ataatttgat aaaagaatta
    10501 aaattaggaa aaattgatgg tatttactta cctaatactc gatggtatag aggcgaagag
    10561 gataaaggtg aatggcaaga gagacttgct aatcaactag ctggcttagc cgatatatat
    10621 gtgaatgatg ctttcggttc atggcagcca cacgtgtcta cctatactat tactaaatat
    10681 ctacctagtt atgcaggatt attattacaa aaagaaatca ataatcttga taaaatcttt
    10741 tcgcctatta aacctatgat agccataata gctggtgcca aatttgatac taaaataaat
    10801 agtttaaaag ctctgataaa agatgtagat tatttaatgc ttggtggtgt tattttaaat
    10861 gcttttttag ctgctaaata caagatatct ataaaaggta tagatgatga taatttgtca
    10921 atggcaaaag aatttgtaga ctttgtccaa actaattatc ctaataagct aatagaacct
    10981 cgttatatag tagaatctga taccatggaa ggtaaaatag aggggcatta caaaatcata
    11041 gatgtgaaaa gcttaaaagc tggtgaaaca ctaaattatg tattagatgt agctcctgaa
    11101 tcttttaatg atcctagtat aatagaaaaa ttcaaatctg cgaaaacttt ttttgtaaat
    11161 gctgtgatgg gctatgctcc acattttaat gatggtacta tagctttaaa tgaattaatc
    11221 aattctaata atgatgctat aaaattatat gccggcggtg atactttgca agagctcaaa
    11281 cgcttattac ctggtatata tatgaaagca ctggatgatt ctaaatttta tatgtttact
    11341 ggtggtggtg cagtattgac tgctatagag cagaattctc cttatggtat ggagcctgta
    11401 aaagcattgc taattgatta gaatatttgc tagattaatt attctctttt tgagttatta
    11461 ttttaaaaat tactttttat aattttttgt aatattagct tattaatgcg ttatattgat
    11521 ttattatgct gaatataaaa aaaattttaa tttctagaac agacaatatc ggagatttag
    11581 ttttgacatt gccattagct ggttttctta aacaaaattt tcctgatata cagatagcat
    11641 atttagtaaa agattatact aagccaatag ctgactctta caaatacatt gatgaagttt
    11701 actcgataga taagataaat gaagaaacta aagaaaaaga aagaataaag ctcataaatt
    11761 caatagatgc tgatgtaatt atttttgttt atccagatag agaagtaatg agtttagtag
    11821 ctaaatcaaa tatatctcga cgtatagcta cttcacatcg tacatattca tggagatatg
    11881 caagtaattt agttaatttt tctagaaaaa attcatattt acatgaagct caattaaatt
    11941 ttttattact aaaaccactt caaatcgatt atttaccttc gattaatgaa ttattgcaat
    12001 ggtatggcaa agataaattt tatattaaac ctataccaaa agatttaatc aatgattttg
    12061 caatatctcc ttatcgtcct ataattatac atcctggttc tatgggcagt gctcccaatc
    12121 tatctccaca acatttttta gatttaacta acaaattagt agaattaggc gaaaatattt
    12181 atattacagg taatgaaaaa gaaggtttgt tatggcaacc atattttgac aaattaaatg
    12241 ttaaaacttt gtttggttca ttaaatttat atgaattgat aagtatcata tctaaagcca
    12301 aggctgtcat agctagtagc acgggtccat tacatatagc agcagctctg gatacaaatg
    12361 ctattggatt gtatgtgcca ataggcactt atgcacctca tagatggtcg ccaataggca
    12421 aaaatgctaa ggtgttaact ccagcttatc ataaaattag ttgtaaaagt aaatgtataa
    12481 gtccaagtaa ttgtacttgt ataaatgaaa taaaaataga aaaaattata aatgaaatat
    12541 atgatattga gacagagtaa tagctcattt attatgcaaa ttgtatttta attttttata
    12601 caaaaaaatt tagtattaaa tcacttggcc gacgtcaatt aatgattatt agtaaattca
    12661 tgtctaaata tatatatgta aagaatatag aagagtgtaa gagataaatg taaaataata
    12721 ttattttaca aattttcaat attaatacaa taaaaaaata agagaaaaag aattagatat
    12781 gatcaaaagg gattgtattt aaagggaata taaaactaat atatgttaaa gtttacatat
    12841 gttatattca ctaactctct ataccgatac cacgcccacc tgtccgagga tgcaagattg
    12901 agcattcgcc cagtattgct tacagtcttg cctgtgaggt atttacccat cactacatac
    12961 cacataatgt agttttgcag gtatttggta gctacgcccc taaatttgaa atatatccaa
    13021 ttcgtgaatt cactagcctt tttcttgaca g
//
LOCUS       DAOPRN010000052        12961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_4065_length_12961_cov_116.709902, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000052 DAOPRN010000000
VERSION     DAOPRN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4065_length_12961_cov_116.709902"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..3360
                     /locus_tag="PKI65_06370"
     CDS             49..3360
                     /locus_tag="PKI65_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOB78101.1"
                     /translation="MSIKVLEASAGSGKTYQLSMFYVKLALENPNNFKKILAITFTNA
                     AVNEMKLRILDRLYSIANNNPNSLEEFKAFAKGGQINGKNITQLSDDDISGAAKNVLY
                     NILHNYQDFSVSTIDSFLQRLFRGALYEIGVKYNYELIVKNDDIYREAVDDFIVNLQE
                     QYPAFNWLIKFIEDKLAQDKSFNYNNMLLDLIKEINKEFFYDYEDDFINKLTDEDFKQ
                     KCLDDIIKCLNNVMNDFLDKAKKINDDFKNICKNNNNITENDFYQKARGPASFLGSKI
                     ENFKIGYEIKKLLPNSYFIEGLNGKLFSGGNTITLKNNDLQNIKDLLFSYNDLINNEG
                     KNYETARIISNQIYNIALLSDILKSLYDYKKTNNILLLSDIGKMLRKFIENNYMFIYE
                     KLGVYYEYFLIDEFQDTSHVQYEIIKPLINESLSQAQSDNVLLVGDIKQAIYRWRNGD
                     WEIMKDTIDTDFHNSISRNNLKENWRSYPNIVNFNNSLFSELLQLTDLFKEKDEDISN
                     PRYQTKINYIKEIYIDTDKNRDVKQEIPDAKKDQFKDFQGYVKLYIAKDKNNKNTTTN
                     EEKSTTYDTSDSIESEEDDKVLQLVINDVNNLWEKGYKNIGILVRTNKEAVEVFKYIL
                     QNSTIQDSDFKIISDESISFANSDAVLWIVFTLYALQNGSNYARYMSEAFYDFIKTSN
                     SVPYNSLMNNLENDDNFMAQNLYFKAEHLVNIIYKELDDKEKVFCMHFLQLIKNYQRE
                     HNNNEVMFIEWFLNNGVKQSLKITDEKNGVHISTIHKSKGLEYDAVIVPFINWTRNQN
                     DYLWFKKPDICNSNIPAFLLKTSGDLANSQFADEYYMEKTKKYVDDLNLLYVALTRAK
                     KVLIANIENQNIGKEIQKCVTNNFNIDGLGAIDTYKSINSNENFDIYEIGTLVNNTTY
                     ASDVSIENFQWAAKENVGIEIKIKKNYSLSSNEKIAHGVLMHDILRVIDDVDNWEKKA
                     DKILKKYHKTSEEIKKIKDNIKTIFEINPEVKNWFTNAQEIISERDLSIYKLESDKDK
                     LESDKERKMYRPDKIFIYPDKVIVVDFKTGEKDLNEYKYQVRNYIEILRQIFDKDIEG
                     FLLNIDNNELLKVEI"
     gene            3386..6193
                     /locus_tag="PKI65_06375"
     CDS             3386..6193
                     /locus_tag="PKI65_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOB78102.1"
                     /translation="MKAFLQEVAEYIYQKHGDNLKNLTILMPNRRSCIFLKQEIAKLC
                     QKTTFLPEVLSLQDWIDDNSSLTKIEDVVAIFKLFDSFKNIYPNKANLKIEDFYFLGE
                     ILLIDFNDIDNALVDASKLFINIKDENEIKNLFNLDLEPDFKDYFDKLIKYFNKSFSE
                     NSYLDIWNKLHDLYKQYRESLIKDNLAYEGLQIRHFVENEIENSKNINEKIFYFIGFN
                     FITKAEYAIFNKLKDKANFIWEYDDEYVDNNLYSAGKQLSWLIKDFPMPADFNVKTNN
                     LGDDLKNVKDKIKICSFTNEVEMAKYAAQHIKNLEQYQAIILPDDSLLPVVLTSLPDD
                     IKSVNVTMNYSIKNTNAYTLIMQIFDLYNNIRKNNTKILISKQKWLELIYHPFIYKNA
                     NVRKMISDYLYPQKTNNNSNSQEINDFIEINNINTADKPLIDKLLSIINANGTIDFMN
                     HLLELLSYLEENLKTTTEKSSMLKLELEAIRQLYTKLQETNVLFDQYKIVITNIKFLI
                     SLITEILREIKIKLEGEPLEALQVMGLMESRLLDFQKVIILSLNNKIVPGDKYIPTFI
                     SYHFRKHFNLPTQDWREGIDAFHIYRLLQRSENIHLLSSRFIADEECDYSPYLLQLKY
                     RGIDIKNFTDKIGKSSQITEHKIKSDAQNKVNDYLNKNDNSGLSRNAISTYIQCPRKF
                     YFKYIENLTDTDLFPEEAIERELGTLIHQALNKLFNNYKNVDIGILQYIKNKVDTVCD
                     ALLPNKDSIKVLLLKHQLKSMLNKFFDVEINPQTSHFPAEILGIEYTVRWTLNNVKLL
                     GTIDLIIKNDKGVYIIDFKTGSEKAVTYTEAEDLFKQNSKMEYVFQLAFYCYLYCAIN
                     NSNNVFGENIYLKSYNKAYKNELKKDKNVVDFYDEKNEFEDLLTIIIDKLKNDGEFEL
                     TEDQKNCIYCDYQLICKRAGG"
     gene            complement(6378..8564)
                     /locus_tag="PKI65_06380"
     CDS             complement(6378..8564)
                     /locus_tag="PKI65_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOB78103.1"
                     /translation="MENKFSFIVSGMVCTMCVKNVENAIKKINGVKYVSVNLATQKAF
                     VIADENIKFEDIKKAVKEVGYNATTEAPKEDLIEKQFVDARQNMYISLAITIPLMILM
                     ILHMSGIYIPYFLIIELIASSCVIFITGKKMLSNAWIALRHLHTNMDTLLSIGAISAW
                     STNILAIAGLNVLSFGTVAAMLVSLNLVGRYIESKMKNNAVKEIKLLMSMKVESANVL
                     INNKIIEAPIDTVKIDDIIIIRQGEKIPLDGTIVEGRAYINESMVNGEPLPIDKTIGD
                     SVLSGTIVENGLIKVKVEKVGSDTFINQMIKLIEEAQSSKVPIQAMADRITRYFVPIV
                     LVLAILSGVFWFFNYELYLPFLSKISKIFPWIITDGGAISTAIFSFVATLVIACPCSL
                     GLATPMALVNASSVAAKKGLIIKNGEAIQMAKDIDTVIFDKTGTITEGKLTVIDHNLT
                     NEVLNIIANIEEKSIHPLAKALIKFVNENELFRHIDITDIEELSGKGIKGKYNEDIYF
                     IGKPLNANNYSNFMEKGETVLEVTKNDKIIGYIRITDKIKADASDTILQIKNLGCKTV
                     MLTGDNELTAKYISDQIGIDELWAGISPNKKVDIIRKYQMEGGKVMMVGDGINDAAAL
                     KAADIGVAIGTGADLAIESADIIISKGNLNVIPYAISLSKLTFKKIKQNLFWAFIYNV
                     IAIPLAMMALLHPAIAEAAMLISSINVIYNSGKIRKHNKNFIYMPN"
     gene            8890..9936
                     /gene="holA"
                     /locus_tag="PKI65_06385"
     CDS             8890..9936
                     /gene="holA"
                     /locus_tag="PKI65_06385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOB78104.1"
                     /translation="MVSTYTPDFLQQFEKIKFDIRAKNYANIYFAYGKESYFVDQLQH
                     IVEDEILDPSEKDLNLYIIYGKDTTIAEIINIAESYSFLGTYKVISIRETQELNLSNF
                     DLLEKYFQKPMHETILFFGYKDDKLNSKWQTLLNKKTSKVIKFNSKGLYNYQVPNFIQ
                     DYAKSKGYNINPHISRILSDNLGTNLSVVINELEKLFILVPDGGEINMQVVEELTGIN
                     RQYNVYELQNALAKKDIKSIQNITLYLASSEERSALLMVIQNLFNFFTQLLIYKQNKK
                     NKNETELVKMLRLSHSFFLKQYQQADQNYTYASIINIISLLREYDLKAKGVGNRSFEQ
                     SELLKELIVRILKS"
     gene            9933..11048
                     /locus_tag="PKI65_06390"
     CDS             9933..11048
                     /locus_tag="PKI65_06390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOB78105.1"
                     /translation="MIQVLEVKTEKDIKEFIDFPFKIYKGDKNWVPPLKNDEKKMFSP
                     HTNPELEFCDTQFWLAKDNGKTVGRIITMINHKYNEKVNKAYARFFKFESINVEVGEQ
                     LLLFAENWAREKRSTHIHGPLGFSNLDNQGLLIEGFEYLPSIASTYNKVFYKDIFDKL
                     EYQKENDWIEFILYPEDCIDERILKLNETIKKRYNLIVNSFKKRKELQPFIPQVLEVL
                     NQAFAELPYVIPFDEKTGSYYIEKYFSLLNPNLIKIITNKDGEAVAFIVGLPSLSRAM
                     QKANGKLSPWGIYNIFRAINHPKVVDLALTAVLPQLQRQGVAAVLITELQKELIRYGV
                     KEVETTGIFETNTQAVQTWKNFKHIQHKRRRCYVKEL"
     gene            complement(11313..12572)
                     /locus_tag="PKI65_06395"
     CDS             complement(11313..12572)
                     /locus_tag="PKI65_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOB78106.1"
                     /translation="MMDLFEKFNLNDTPLGKYYELAHGYFMYPQLEGEIGPRMKFMGK
                     EMIVWSLNNYLGLANHPEVRKIDADASAEYGLAYPMGARMMSGHTKEHRKLEKELADF
                     VKREDAFVLNYGYQGMFSIIDSLVNRKDVIVYDEEAHACIIDGMRLHIGKRFTYKHND
                     MESLEKQLQHAENLTKETGGGILVITEGVYGMTAELGDIPGIIALKDKYNFRLLIDDA
                     HGIGTMGPTGAGTDEHFGLQSQVDLYFGTFAKAFAGIGAFIAGPRNVINYLRYNMRSQ
                     IYAKSLPMAMVLGIQKRLEMIKTMPELREKLWNNVHTLQNGLRNAGFDLGKTQSPVTP
                     VFLNGGDREASNLVVDLRENYNIFCSIVTYPVVPKGIFLIRLIPSAAHSFEDIQITIK
                     AFNEVKDKLDSGFYKELDLPHKKIIED"
     gene            complement(12699..>12961)
                     /locus_tag="PKI65_06400"
     CDS             complement(12699..>12961)
                     /locus_tag="PKI65_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78107.1"
                     /translation="KRGIYSLSTVKKKASEFTNWIYFKFRGVATKYLQNYIMWYVVMG
                     KYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNKTYVDFNIC"
BASE COUNT         4954 a         1782 c         1828 g         4397 t
ORIGIN      
        1 tttgtttatt gaaatttatt tccttatttt tacaaaaata ttcaataaat gtctattaaa
       61 gtattagaag cctcagctgg tagtggtaaa acatatcaat tgtctatgtt ttacgttaaa
      121 ttagcattag aaaacccaaa taattttaaa aaaatactcg ctatcacttt cactaatgcc
      181 gctgtgaatg agatgaaact gagaatatta gatagactgt attctatagc taataacaac
      241 cccaactccc ttgaagaatt taaagctttc gctaaaggcg gacaaattaa tggtaaaaat
      301 ataactcaac tatctgatga tgatatatct ggtgctgcta aaaatgtttt atataatatc
      361 ctgcacaatt atcaggactt ttctgtatct accattgata gctttttgca aaggctcttt
      421 agaggtgctc tctacgagat aggcgttaag tataattatg aattaattgt aaaaaacgac
      481 gatatctaca gggaggcggt agatgatttc atagtaaatc tgcaagaaca atatcccgct
      541 tttaattggc ttattaaatt tattgaggat aaactcgctc aagataagtc ttttaattac
      601 aataatatgc tattagacct aattaaagag attaataaag aatttttcta tgattatgag
      661 gatgatttta ttaataaatt gactgatgag gattttaaac agaaatgttt agatgatata
      721 attaaatgtt taaataatgt aatgaatgat tttttagata aagcaaaaaa aataaatgat
      781 gattttaaaa atatttgtaa aaataataat aatataacag aaaatgattt ttatcaaaaa
      841 gcaagaggtc cagcttcttt tttaggttca aagattgaaa attttaaaat aggttatgaa
      901 ataaaaaaac ttttacctaa tagttatttt attgaagggt taaatggcaa actttttagt
      961 ggtggtaaca cgattacatt gaaaaataat gatttgcaaa atataaaaga tttattattc
     1021 tcttataatg atttaataaa taatgaaggg aaaaattatg agactgcacg tatcatttct
     1081 aatcaaatct ataatatagc tctgctaagt gatattttaa aaagtctata tgattacaaa
     1141 aaaacaaata atatcttgct tttgtctgat ataggaaaaa tgctaagaaa gtttatagaa
     1201 aataattaca tgtttatata tgaaaaacta ggtgtatatt atgaatattt cttaatagat
     1261 gaattccaag atacctctca tgtgcaatat gaaattataa aaccattaat caatgaaagc
     1321 ctcagccaag ctcagtcaga taatgttttg ctagttggcg atatcaagca agctatatat
     1381 cgctggcgta atggagactg ggaaattatg aaagatacca ttgatactga ctttcataat
     1441 agtatttcta gaaacaatct taaagaaaat tggcgttctt atcctaatat tgtgaacttt
     1501 aataattccc tttttagtga acttttacaa ttaactgatt tatttaagga aaaagatgaa
     1561 gatattagta acccaagata ccaaactaaa atcaattaca ttaaagaaat atatatagat
     1621 actgataaaa atagagatgt taaacaagaa atccctgacg ccaaaaaaga tcaatttaaa
     1681 gatttccagg gatatgtaaa actctatata gcaaaagata aaaataataa aaatactaca
     1741 acaaatgaag aaaaatcaac tacatatgat acatcagact ccatagaatc tgaagaagat
     1801 gataaagtac tacaattggt aataaatgat gtaaataatt tgtgggaaaa aggctacaaa
     1861 aacattggta ttctcgttag aaccaataaa gaagctgtag aagtattcaa atatatactc
     1921 caaaattcaa caattcagga ttctgatttc aaaattattt ctgatgagag tattagcttt
     1981 gctaattctg atgccgtgct atggatagtt tttaccctct atgcactaca aaatggctct
     2041 aattatgcta gatacatgtc agaggctttc tatgatttta taaaaacatc aaattcagta
     2101 ccttacaata gtctgatgaa taatttagaa aatgatgata atttcatggc tcaaaactta
     2161 tatttcaaag cagagcattt ggtaaacatt atttataaag agctagatga taaagaaaaa
     2221 gtcttttgca tgcatttcct acaattaata aaaaattatc agcgggagca caataataat
     2281 gaggtgatgt ttattgagtg gtttctgaat aatggagtga aacagtcatt gaagataaca
     2341 gatgaaaaaa atggagttca tatttctact atccataaat ctaaaggatt agaatatgac
     2401 gctgtgatag tacctttcat caattggact agaaatcaaa acgattatct atggtttaaa
     2461 aaaccagata tttgcaattc aaatattcct gcatttttat taaaaactag tggagatctt
     2521 gcaaattctc aattcgcaga tgaatattat atggaaaaaa caaaaaaata tgtggatgac
     2581 ctcaatctgc tctatgtagc tctaactcgt gcaaaaaaag tgctcattgc taatattgaa
     2641 aatcaaaata taggtaaaga aatccagaaa tgtgtaacta ataattttaa catagatggt
     2701 ctaggagcaa ttgatactta taaatcaata aattctaatg aaaattttga tatttatgaa
     2761 attggaacat tagtaaacaa tactacctat gcatcagatg tgtctataga aaatttccaa
     2821 tgggcagcaa aagaaaatgt gggcatagaa ataaaaatta agaaaaatta tagtttatct
     2881 tcaaatgaaa aaattgctca tggtgtattg atgcatgata ttctcagagt aatagacgat
     2941 gtagacaatt gggagaaaaa agctgataaa atattaaaaa aatatcataa aacatcagaa
     3001 gaaattaaaa aaattaaaga caatataaaa actatttttg aaattaaccc agaagtgaaa
     3061 aattggttta ctaatgcaca ggagattatt agtgagcgag atttgtctat ttataagcta
     3121 gagtcggata aggataagct agagtcggat aaggaaagaa aaatgtatag accagataag
     3181 atatttattt atcctgataa agtcattgtt gtagatttca aaactggtga aaaagattta
     3241 aatgaatata aatatcaagt gagaaattac atagagatcc tacgtcaaat ttttgataaa
     3301 gatatcgaag gatttttgct taatatcgac aataatgaat tgctaaaagt agaaatataa
     3361 taattaatta aaaactcaaa aaaatatgaa agcatttttg caagaagtag cagaatatat
     3421 ttaccaaaaa catggtgata atctaaaaaa tctaactata ttgatgccca atcgtcgctc
     3481 atgtatattt ctaaaacaag aaatagccaa actttgtcaa aaaacaactt tcttgcccga
     3541 agtactttct ctgcaagatt ggattgacga caattcatct cttactaaaa tagaagatgt
     3601 agtcgccatt ttcaaattat ttgattcttt taaaaatatt tatcctaata aagcaaatct
     3661 aaaaattgaa gatttctatt tccttggtga aatactgctt atagatttta atgatattga
     3721 caatgctctg gtagatgcta gtaaactatt tataaatatt aaagatgaaa atgaaataaa
     3781 aaatcttttc aatttagatt tggaaccaga ttttaaagat tattttgata aattaattaa
     3841 gtattttaat aaatccttta gtgaaaattc atatttagat atttggaata aattacatga
     3901 tttatacaaa caatatagag aatcattaat caaagataat ctagcttatg aaggtctaca
     3961 aattagacat ttcgtagaaa atgaaataga aaattcaaaa aatatcaatg aaaaaatctt
     4021 ttattttata ggttttaatt ttattacaaa agcagaatac gcaatattca ataaattaaa
     4081 agataaagca aattttatct gggaatatga tgatgaatat gtagataaca acctttatag
     4141 tgctggtaag caattgagtt ggttaattaa ggattttcca atgcccgctg attttaacgt
     4201 taaaactaat aatctaggcg atgatctaaa aaatgtaaaa gataaaataa aaatttgctc
     4261 ttttactaat gaggtggaga tggctaaata tgctgctcaa catattaaaa acttagaaca
     4321 atatcaagcg atcattttgc ccgatgatag tctgctacct gtggtactca cttctctgcc
     4381 agatgatatc aagtctgtaa atgtaacgat gaattattct attaaaaata ctaatgctta
     4441 cactctcata atgcagattt ttgatttata caataatatt agaaaaaaca atactaagat
     4501 tttaatatct aaacagaaat ggttagagct gatatatcat ccttttatct ataaaaatgc
     4561 taatgtacga aaaatgatta gtgattattt atatcctcaa aaaactaata ataattctaa
     4621 tagtcaagaa atcaatgatt ttattgaaat aaataatata aatactgctg ataagccatt
     4681 gattgataaa ttgctatcta tcattaatgc taatggcacg atagatttca tgaatcattt
     4741 attggagttg ctatcgtatt tagaggaaaa tttaaaaact acgacagaaa agagttctat
     4801 gcttaaattg gagttagagg ctattagaca attatatact aaactacaag aaaccaatgt
     4861 tctatttgat caatataaaa tagtaataac aaatatcaag tttctaatat cattgataac
     4921 agaaatatta agggagataa aaataaaact tgaaggtgaa ccattggaag ctcttcaagt
     4981 gatgggactg atggagagtc gtcttttaga tttccaaaag gtaataattc tttcactgaa
     5041 taataaaata gttcctggag acaaatacat acctacattt atctcatatc atttcaggaa
     5101 acattttaat ttaccaactc aggactggcg tgagggcata gatgcttttc atatttatag
     5161 attactacag cgtagtgaaa atattcacct actatcatct aggttcatag ctgatgaaga
     5221 gtgcgattat agtccgtatt tattgcaatt aaaatatcgt ggtatagata tcaaaaattt
     5281 cacagataaa ataggcaagt catcacaaat aactgaacat aaaataaaaa gtgatgccca
     5341 aaataaagta aatgactatc tgaataaaaa tgacaatagc ggattatctc gtaatgccat
     5401 tagcacttac atacaatgtc cacgtaaatt ttatttcaaa tacatagaaa atctgacaga
     5461 tactgatcta tttcccgagg aagcaataga aagagaatta ggcactctga tacatcaagc
     5521 tttaaataaa ttatttaata attataaaaa tgtagatatt ggaatattgc aatacattaa
     5581 aaataaagta gataccgtat gtgatgcctt attacctaat aaggatagta taaaagtgct
     5641 actgctaaaa catcaattaa aaagtatgct aaataaattt tttgatgtag aaataaatcc
     5701 tcaaacatct catttccctg ctgagatttt aggaatagag tacacagtac gttggacatt
     5761 aaataatgtg aaattactag gcaccataga tttgataatt aaaaatgata aaggggtata
     5821 tatcatagat ttcaaaacgg gtagcgaaaa agcggtaact tatactgagg ctgaggatct
     5881 ttttaagcaa aattctaaaa tggaatatgt ttttcagcta gctttttact gctatttata
     5941 ttgtgctata aataattcta ataatgtttt tggagaaaat atttatttaa aatcttataa
     6001 caaagcatat aaaaatgaat taaagaaaga taaaaatgtt gttgattttt acgatgaaaa
     6061 gaatgaattt gaagatttat taacaataat aatagataaa ttaaaaaatg atggagagtt
     6121 tgaattaact gaagatcaaa aaaattgcat atattgtgat tatcaattga tttgcaaaag
     6181 ggcagggggg tgagaggtac tacttataac tgacaactaa caacctacaa caaatgtatt
     6241 gctcaacttc taaactatta tttatgtgtt tctatgtaaa aatcataaat tattttttac
     6301 ataccaccca cttttcacta cgtttaatat ttttattacc attaccatat atttattaaa
     6361 tgttttattt tgtagattta atttggcata tagatgaaat ttttattatg ttttctgatt
     6421 ttaccagaat tgtagataac atttatagaa cttattagca ttgctgcttc ggctattgca
     6481 ggatgtagta gagccatcat ggctaaaggt atagctatga cattataaat aaatgcccag
     6541 aaaaggtttt gtttaatttt tttgaaagtt aatttagaaa ggctaatggc gtaaggtatc
     6601 acattaagat taccttttga gatgataata tctgcacttt ctatagctag atcagcacca
     6661 gtaccaatag ctacacctat atcagcagct tttagagctg ctgcatcatt tataccatca
     6721 cctaccatca taacttttcc cccctccatt tggtattttc taataatatc aactttttta
     6781 ttaggtgaaa tgcctgccca taactcatct atacctatct gatctgaaat gtatttagct
     6841 gttaattcat tatcaccagt gagcattaca gttttgcatc ctagattttt tatttgtagt
     6901 attgtatcag acgcatcagc tttgatttta tcagttattc ttatataacc aattatttta
     6961 tcatttttag taacttctaa tactgtctct cccttttcca taaaattact ataattattt
     7021 gcatttaaag gtttaccaat aaagtaaata tcctcattat atttaccttt tattcctttg
     7081 ccacttaatt cctctatatc agtaatatca atatgtcgaa atagctcatt ttcatttaca
     7141 aattttatca gtgctttagc aagtggatgt atagattttt cttctatatt agctataatg
     7201 tttaggactt cattagttaa attatggtct attactgtta attttccttc agtaatagtg
     7261 cctgttttat caaaaattac agtatcaata tcttttgcca tttgtatagc ttctccattt
     7321 ttaataataa gtcctttttt agcagctaca ctactagcat tcacaagagc cattggtgta
     7381 gctaagccca atgaacaggg acaagcaatt acaagtgtag caacaaaaga aaaaattgca
     7441 gtagatatag caccaccatc agtaatgatc catggaaaaa tttttgaaat ttttgataaa
     7501 aaaggaagat aaagttcata attaaaaaac cagaaaacac cacttaaaat tgctaatact
     7561 aaaactatag gaacaaaata gcgagttatt ctatcagcca tagcttgtat tgggacttta
     7621 gaagattgcg cttcttctat aagtttaatc atttgattaa taaaagtatc agatcctact
     7681 ttttctactt ttacttttat taaaccattt tctacaatag taccactgag tacactatca
     7741 ccaatggttt tatctattgg tagtggttcg ccattaacca tggattcatt tatataggct
     7801 cgcccctcca caatagtacc atctagtggt attttttcac cttgcctgat aataattata
     7861 tcgtctattt ttacagtatc tatcggggct tctattatct tattatttat taaaacatta
     7921 gcactctcta ctttcattga catcaagagt tttatttctt tgacggcatt atttttcatt
     7981 ttggattcaa tatatctacc taccagattc aaagacacta acatagctgc cacagttcca
     8041 aatgataaaa catttaaccc tgctatagct aaaatattag ttgaccaagc tgagatagcc
     8101 ccaatagata ataaagtatc catatttgta tgtaaatgtc taagtgctat ccacgcatta
     8161 cttaacattt ttttaccagt tataaaaatt acacaggagc tagctattag ttcaattatt
     8221 aaaaaataag gaatatatat accactcata tgaagtatca taagtatcat taatggaata
     8281 gtgatagcta atgatatata catattctgt ctagcatcta cgaattgctt ttctattaaa
     8341 tcttcttttg gagcttcggt agtggcatta tagccaactt ctttaactgc ttttttaata
     8401 tcttcgaatt tgatattttc atcagctatt acaaatgctt tctgagtagc gagatttaca
     8461 gatacatatt tgacgccatt gatctttttt atagcatttt ctacattttt tacacacata
     8521 gtacatacca ttcctgatac aataaatgaa aatttatttt ccataatata ttattaattt
     8581 ttttcaaaat tataacatat ttaaacaaat aaaaaagata aaatacaaag ggcaaaagat
     8641 agattaatat ggtaaagtaa agaaacgtta ctaaaatagt agatcctaat attttgaaag
     8701 atttctatat tttgtcaaaa tgatattata ctgagatgat tattcaaaaa atcatattat
     8761 taataaatga aaatcaagat cgataatgtt aattttttct taacttttag aaataatttc
     8821 atttattaat tatttgtatt tttgtcatca taaattttgt ttaataatta atataaaatc
     8881 atcataataa tggtttctac atatacacca gattttttac aacaatttga aaaaataaaa
     8941 tttgatatac gagctaaaaa ttatgctaat atatattttg cttatggtaa agaatcatat
     9001 tttgtagatc aattacagca tatagtagaa gatgaaatct tagatccgag tgaaaaagat
     9061 ttgaatttat atattattta tggtaaagac acaactattg cagaaatcat aaatatagct
     9121 gaaagctatt catttttagg cacttataaa gttatttcta tcagggaaac tcaagagctg
     9181 aacttatcaa attttgattt actcgaaaaa tattttcaaa aacctatgca tgagactata
     9241 cttttctttg gttataaaga tgataaactc aatagtaaat ggcaaacatt acttaataaa
     9301 aaaacctcga aagtaataaa atttaatagt aaaggtttat ataattatca agtaccaaat
     9361 tttatccaag actacgctaa atccaaagga tataatatta atccgcatat cagtcgaata
     9421 ttatcagata atctcggaac aaatttgtct gtagttatta atgaattaga aaaattattt
     9481 attctagtgc cagatggcgg cgaaataaat atgcaagtag tagaagaact cacaggaata
     9541 aatagacagt ataatgtgta tgaattacaa aatgctttag caaaaaaaga tattaaaagt
     9601 attcaaaaca taactttgta tttggcatcg agtgaggaga gaagtgctct attaatggta
     9661 atacaaaatt tgtttaattt ttttactcaa ttacttattt ataaacaaaa caaaaaaaac
     9721 aaaaatgaaa cagaactggt aaaaatgtta cgattatctc attcgttttt tttaaaacaa
     9781 tatcaacaag ccgaccaaaa ttatacttat gcatctatta taaatattat atctctactt
     9841 cgagaatatg atctcaaggc taaaggtgta ggtaatcgat ctttcgaaca aagtgaatta
     9901 ttaaaagaat taattgttag aattttaaaa tcatgataca agttttagaa gtaaaaacag
     9961 agaaagacat aaaagagttc atagattttc ctttcaaaat atataaaggt gacaaaaatt
    10021 gggtaccacc tctgaaaaat gatgaaaaaa aaatgttttc acctcataca aatccagaat
    10081 tagaattttg tgatacacaa ttttggttag ctaaagataa tggcaaaacc gttgggcgta
    10141 taataacgat gataaatcat aaatataatg aaaaggtaaa taaggcttat gcacgttttt
    10201 ttaaatttga atcaataaat gtcgaagtgg gggaacaatt actattattt gctgaaaatt
    10261 gggcaagaga aaaacgttct actcatattc atggtccttt aggtttttcc aatcttgata
    10321 atcagggttt attaattgaa ggtttcgaat acttaccatc tattgcttct acatataata
    10381 aggtatttta taaagacatt tttgataaat tagaatatca aaaagaaaat gattggatag
    10441 aattcattct atatccagaa gattgtatag atgaaaggat attaaaatta aatgaaacta
    10501 taaaaaaaag atataattta atagtaaact catttaaaaa acgaaaagag ttacaaccat
    10561 ttataccaca agtgttagaa gtattaaatc aagcttttgc tgaacttcct tatgtaatac
    10621 cttttgatga aaagacaggt agttattata tagaaaaata tttttcttta ttaaatccga
    10681 atcttataaa aataattaca aataaagatg gagaagcagt tgctttcata gtaggtttac
    10741 catcattatc acgagctatg caaaaagcaa acggaaagct ctcaccctgg ggtatataca
    10801 atatatttcg agctataaat catcccaaag ttgtagattt agcattgaca gcagtattac
    10861 cgcaattaca gagacaaggc gtagcagctg tattgatcac tgagctacaa aaagaattga
    10921 tacgttatgg agtaaaagaa gtagagacta caggaatttt tgaaactaat acacaagcag
    10981 tacaaacctg gaaaaacttt aaacatattc agcataagcg tagacgttgt tatgtaaaag
    11041 aattataata gttattttaa atataacgtt ttatttataa cctgattttt atgcaaatta
    11101 ttctttaatt atacataagt attgcttaaa tatttctcat taactactat ttcaagggta
    11161 tatgtatatt ttgagtttag aaaatattaa aaaaaataag aataattaac taattaatac
    11221 gcaaataatc aatataattt ttattaatat attaaatttg tattataaaa cagaagggtg
    11281 cttagtgtaa tttttttata atttctatta tctcaatctt caataatttt tttatgaggt
    11341 agatctaatt ctttataaaa gccactatct aatttgtctt taacttcatt aaaagctttt
    11401 atagtaattt gaatatcctc gaaagagtga gctgcagaag gaataagtct aataagaaaa
    11461 attcccttag gaacaactgg gtaagtaaca atagaacaga aaatattata attttcgcga
    11521 agatctacta ctaaattaga agcttctcta tcaccaccat tcaaaaatac aggagtaaca
    11581 ggagattgag ttttgccaag atcaaaacca gcatttctca atccattttg caatgtatgc
    11641 acattattcc aaagcttttc acgtagttca ggcatagttt ttatcatttc taagcgtttt
    11701 tgaataccta aaaccatagc cataggaagg gatttagcat atatttggga tctcatatta
    11761 tagcgtaaat aattaataac atttctaggc ccagcaataa atgcacctat gccagcgaaa
    11821 gcttttgcaa aagtaccaaa atacaggtct acttgagatt gtagtccgaa atgttcatct
    11881 gtacctgctc ctgttggtcc catagtgcct atgccgtgag catcgtctat gagtaaacga
    11941 aaattatatt tatcttttag ggctataata ccaggtatat cacctaattc agctgtcatt
    12001 ccataaacac cttctgtgat tactaaaatt ccacctccag tttcttttgt taaattttca
    12061 gcatgttgca gttgtttttc taggctttcc atatcattat gtttgtaggt aaaacgctta
    12121 cctatatgta aacgcatccc atctattatg caagcatgag cttcttcatc atatactatc
    12181 acatcttttc tatttaccaa gctatctatg atagaaaaca taccttgata tccataattt
    12241 aatacaaaag catcttcgcg tttaacgaaa tcagccaatt ctttttctaa ttttctatgt
    12301 tcttttgtat gtccactcat cattcttgca cccattggat aagctaatcc atattcagca
    12361 gatgcatcag catctatttt acgaacttca ggatgattag ctagacctaa ataattattt
    12421 agactccata caatcatctc tttacccata aacttcatac gaggtccgat ttccccttct
    12481 aattgaggat acataaaata tccatgtgct agttcataat atttgcctag gggtgtgtca
    12541 tttaaattaa atttttcaaa aagatccata atttttttgc aaagttggtt aatttttttt
    12601 aaatagaaaa tatttgcagt ttttgataaa taaaaacaag agtttttatt taaaatatta
    12661 agagggaaat tgtttagaaa attaatcaat tcggaaaact aacatatgtt aaaatcaaca
    12721 tatgttttat ttactaactc tctataccga taccacgccc acctgtccga ggatgcaagg
    12781 ttgagcattc gcccagtatt gcttacagtc ttgcctgtga ggtatttacc catcactaca
    12841 taccacataa tgtagttttg caggtattta gtagctacgc ccctaaattt gaaatatatc
    12901 caattagtga attcactagc tttcttcttg acagtactta ggctatatat acctcgcttt
    12961 t
//
LOCUS       DAOPRN010000053        12370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_4334_length_12370_cov_109.533821, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000053 DAOPRN010000000
VERSION     DAOPRN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12370
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4334_length_12370_cov_109.533821"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..3313
                     /locus_tag="PKI65_06405"
     CDS             236..3313
                     /locus_tag="PKI65_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOB78108.1"
                     /translation="MEYNFREIEKKWRTYWKMSQIYKVDIDYDKQKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYARYKRLQGYNVLHPIGFDAFGLPAEQYAIQTGQHPAITTEK
                     NIHRYREQLDLLGFSFDWSREIITCDPKYYKWTQWTFIKLFKSWYDKNIEKARPIDEL
                     INIFETNGNANVNAATTQTKIFTKEAWTNYNEKEKQEILMNYRLAYLSETFVNWCPAL
                     GTVLANDEVSDGFSIRGGYPVERKKMKQWFLRITAYADRLLSGLEKIDWPEHIKEIQR
                     NWIGRSEGALIRFPIKGIQDKYIEIFTTRPDTIFGVSYICIAPESDLVPLITKPSEND
                     KVKNYIKKSMNKSERERLTDIDEKTGVFTGSYAIHPFTGEEIPIWVADYVLISYGTGA
                     VMAVPAHDNRDYAFAKQFGLPIKEVIKGGNIAKEAYEDLKGECVNSDFINGLKPSDAI
                     KKIIEEVEKRGIGTRKVNYRLRDAIFSRQRYWGEPFPIYYKDGIPYTLDESELPLELP
                     LVDSYLPTEDGEPPLARAKNWKTKDGYPLETCTMPGFAGSSAYYLRYMDPNNDNELVS
                     KKAVEYWRNVDLYIGGSEHATGHLIYARFWNKFLYDISVAAEDEPFFKLINQGMILGR
                     SNFVYRAKPLDFAIYKLKNVLPAISDNYEINLQDDIIIIFLQNYKTYLLIVEKDDIDN
                     AKALANSLVTNNYKILIINITKILLKDNLKEFLENQINNLQDKEIKIIDEDIDTPPIF
                     VSKNIEGREEYTYPMHVDISLVRNDILDIEAFKKWQPEFEHAHFILELDKYVCGWEIE
                     KMSKSKYNVQNPDDVVGQYGADTLRLYEMFLGPVEQSKPWDVAGIEGVHRFLRKFWRL
                     FFDDKDDFYLNDNEPNRDELQILHKTLKKVQEDIERYAYNTAISTFMVAVNNLQDLKC
                     NNRKILEPLIIALSPFVPFITEELWHLAGHTDSIHKAKFPDYDEKYTIDNTCTYAISF
                     NGKTRFTLDIDASADEETIKEIILTHPQSEKWLKGKNPKRVIVVKGKIVNVVV"
     gene            complement(3449..4927)
                     /locus_tag="PKI65_06410"
     CDS             complement(3449..4927)
                     /locus_tag="PKI65_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78109.1"
                     /translation="MLKKLCLLNILIIFINSLFSQVLIKQSNTLENLYTDVVLSNNGQ
                     YAYLTNIDGEFCILDMIKMDINKRLKEHTGFIKSIVMDDKNRLYTAGGDKMIIQWDAS
                     TGNVLKKVLTPHYNKINDLAISKNGKYLVTGSEDKSVLVYWADSLVLYKKYIPNSSAV
                     ACVSISPFNEWVVSGGWDHKIVFTSLKTDEMFTLNGHKGAVLDIDFTPDGKYLISGST
                     DNTAILWDVKNKTKLATFKSKGGSVNNVECFFDNRYAAFTDDLGYIHIINIQERRKIA
                     ETQIANSSIEGINLAYPIGWMGIITSDKKLYIYNMNQFILDSCYKSNITEFDSLSAPK
                     KITETDQQYIARLQQFAARQLTVLNKCYAEATKIRNLQAKKKDTLFAMQYHEIEIPID
                     SIGQYDDKNFVLQIKVNGQWYDIKLPIQDAQSLLTNYQKSTVLAIKRPIIDDNPYMPD
                     YQIINMRLKHPISNKIYPIGEQIIPADDKYLRIYLQLQAKRN"
     gene            complement(5036..6124)
                     /gene="hydE"
                     /locus_tag="PKI65_06415"
     CDS             complement(5036..6124)
                     /gene="hydE"
                     /locus_tag="PKI65_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOB78110.1"
                     /translation="MKSILDKQDFSNLDKNEIIELLQLRGSDELNLFKKANKIRLRYR
                     GDRVYLRGIIEYSNICKKNCLYCGIRSGNKNLTRYTIKDEEFYEAVNFAIKHRYGSIV
                     IQSGENTSTAFIDKMTEYIKEIKRISIGKLGITLSLGEQSKETYQRWYEAGAHRYLLR
                     IEESHPELYKKLHPQDDIHDFDKRYRCLVDLKEIGYQLGTGIMIGLPYQTIEHLADDL
                     IFMKNLDIDMCGMGPYIEHPDTPLYKYKNNLLPIKERFSLTLKMIAILRLMMPDINIA
                     ATTAMQAIDPMGREKALQAGANVLMPNITPKKYRDEYLLYKNKPNIDKNPDDNICCIE
                     SRIRIAGFRIGYDEWGDSLHYFRNKKGI"
     gene            6400..7290
                     /locus_tag="PKI65_06420"
     CDS             6400..7290
                     /locus_tag="PKI65_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOB78111.1"
                     /translation="MKKYYIVLLSLLFLTNTYAQRNEVGISGGGMYYIGDLNPYYHFY
                     FTRPHIGLYYRYNYKDYLSVRIFGNYGSVYAADSVLNYYPERNLHFKSPILEIGVVGE
                     INYYKFRPGARSHTFTPYLFLGINWFYFNPKAKYQDEWINLQAMGTEGQGTTLYPERK
                     PYSLNSFAIPMGIGIKIALSEHFICHLEWGMRRTFTDYLDDVSTTYPDPLILATQIDP
                     IAPYLSDPNLLEYNELRNNNIDKWRGDPSTKDWYSVINLGFSFRFKNKSGHCEGMTPR
                     YRDKIKDYRDNLKSKEKIRF"
     gene            7297..8013
                     /locus_tag="PKI65_06425"
     CDS             7297..8013
                     /locus_tag="PKI65_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOB78112.1"
                     /translation="MKQNLFNNLPLHVAIIMDGNGRWASIRGLPRVKGHEAGIDAAKK
                     IINKAGEMGIKYLTLFAFSIENWGRPQDEVMYLFSLFDKAINTYSDELIANGVKVKFM
                     GTLSLLPPDIQEGVSLLESQTADNNHLTLVIAMSYGSRQEIVDATKAISQKILNDQMT
                     IDDINVDSFRQYLYLPELPDPDLIIRTSGEKRFSNFLLFQSAYSELYFTDTLWPDFDE
                     SEFEEAIADFYNRKRRYGKI"
     gene            8121..10634
                     /gene="bamA"
                     /locus_tag="PKI65_06430"
     CDS             8121..10634
                     /gene="bamA"
                     /locus_tag="PKI65_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOB78113.1"
                     /translation="MYKKTLALFLLLFIITFSSIGQIIDIDYRLPKEYIIGGVTVSGL
                     QNEESKDLIILISDLVPGKKITIPGEDIQKAIKNLYKQGMFETIEISVVSRAANTIYL
                     DIYIEEKDRVSSFVIEGVKKNEANSLKEKINIQTGDIITEFLIENVKNKIEKHYIDKG
                     FHNIKVKIDQIPDTSAKGRSTLYINIDKGPRVKIEEINFEGNQVLTDKKLKKAMKDTK
                     EKNIFNIFKSSKFILANYEKDKANIINKYAELGYKDAIILKDSIYPINNKSLKIDIYL
                     EEGKKYYFRNITWVGNTKYSSETLNQILQIKKGDTYNEKLLQTNLFANPNGLDVAALY
                     MDNGYLFFQMNPVIVNIENDSIDIEIQIYEGKQATINKVSVIGNTVTNDRVILREVRS
                     KPGDLFKRSDIIRTQMELAQMRFFNQEELNVNMNPDPSAGTVDLEYVVEEISTNQIEL
                     NGGWGLGRFVGTLGLVLNNFSLKNIFKKDAWRPIPSGDGQILAIRAQTNGQYYQAYSF
                     SFMEPWLGGKKPNALSISIYHSLESNGISKKKDGTYNSEGTLLQRQEISITGASVGLG
                     LKLKKPDDFSRLFITVNYQHYKLDKYYQVLTFQNGVANNFNIGVSFSRNSTDSPIYPK
                     SGSSIYLSLNITPPYSFFKKKNWSELPDSVKFRWIEYHKWKIELENYTNVYGKWVLMA
                     RAKFGLMGMYNRDIGLSPFERFYLGGDGLSGFSLDGREVIGMRGYANQVLTPRDPKGN
                     YIGGTIYAKYTLEMRYPLSMNPMATIYLLGFVEAGNAWVNIKEFQPFNTLKSAGIGVR
                     LYMPMFGLLGLDFGIPFDDTFANKKYQGQFHFSINASID"
     gene            10648..11163
                     /locus_tag="PKI65_06435"
     CDS             10648..11163
                     /locus_tag="PKI65_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOB78114.1"
                     /translation="MKKLIPLILLVFFSVGLIAQSPTKIGFVDTQYILEQMPAYQNAI
                     NQLDNLSAQWQQELEQKYAEIDQMYRKYQAEAYLLPQNEKIKRENEIVEAEKAAKELQ
                     KKYFGPEGELFQRRKALLQPIQNQLYEAINDYAKKNRMGAIFDISNNTTLLFYDNKLD
                     ITNDIKMNLGL"
     gene            11241..11753
                     /locus_tag="PKI65_06440"
     CDS             11241..11753
                     /locus_tag="PKI65_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOB78115.1"
                     /translation="MKKIPLVIILSVFLSFALMGQQTKLKIGHINTNELMQLMPGRDT
                     AEQVLTVYAQNLQKQLEAMVEEFQTKYSDYLANENQFIDAIKQVKQKELLDLQTRIED
                     FQEEAQKLLVAKEQELLNPLIDKAKKAIEEVAKEKGYTYILDTGTGVVLYWESGDDIM
                     SFVKDKLGIK"
     gene            11855..11929
                     /locus_tag="PKI65_06445"
     tRNA            11855..11929
                     /locus_tag="PKI65_06445"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11890..11892,aa:Glu,seq:ttc)
     gene            11959..12234
                     /gene="rpsT"
                     /locus_tag="PKI65_06450"
     CDS             11959..12234
                     /gene="rpsT"
                     /locus_tag="PKI65_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOB78116.1"
                     /translation="MAHHKSAKKRIRSNEKKRLYNRYYNKTMRTMVKKFYATADPQEA
                     AQMLPSLYSIIDKNVKRGIIHKNKGANLKHKITKELNKRLNSAATTA"
BASE COUNT         4482 a         1692 c         1904 g         4292 t
ORIGIN      
        1 gatcttttaa tatagttttc ataattataa attttggtaa aattaacaat tatttttata
       61 ttaatccaat actaaattta acaatcatga ttttttcaaa gcgatacctc cacttcccac
      121 gttacaatct ttcaataaat cttttattat tactatcaat atccgtaaat ctctcaaatg
      181 aaatgttatt ttctttaaat ttctgtaatt ttgcaattca aaatattact aatttatgga
      241 atataatttt agagaaattg agaaaaaatg gagaacttac tggaaaatgt cacaaattta
      301 taaggtagac attgattatg ataaacagaa atattatgta ttagacatgt ttccatatcc
      361 gagtggtgca ggtttgcatg tgggacatcc attggggtat atagcttctg atatttatgc
      421 tagatataaa agattgcaag gttataatgt gttgcatcct ataggttttg atgctttcgg
      481 tttgcctgct gagcaatatg ctattcagac tgggcaacat ccagctatta ctacagaaaa
      541 aaatatacat agatatagag agcaattaga tcttttagga ttttcttttg attggtcacg
      601 agaaatcatt acttgtgatc ctaaatatta caaatggacg caatggactt tcattaagct
      661 tttcaaatct tggtatgata aaaatattga aaaagccaga cctattgatg aattaataaa
      721 catttttgaa acaaatggta acgctaatgt aaatgctgct actactcaaa caaaaatttt
      781 tactaaagaa gcttggacta attataacga aaaagaaaag caagaaattt taatgaatta
      841 tcgactagca tatctatcag aaacttttgt aaactggtgc cccgctttag gaactgtatt
      901 agccaatgac gaagtatctg atggttttag tatacgtggt ggatacccag tagagcgtaa
      961 aaagatgaaa caatggtttc tgcgtataac tgcttatgcg gatagattat tatcaggatt
     1021 agaaaaaatt gattggcctg agcacattaa agaaatacaa cgcaattgga ttggccgctc
     1081 cgaaggagct ttgattagat ttcccataaa aggcattcaa gacaaatata tagaaatatt
     1141 tactactcgt ccagacacca tctttggtgt ttcttatatc tgcatagctc cagagtctga
     1201 tctagttcct ttgattacta aaccatcaga aaatgataaa gtaaagaatt acatcaaaaa
     1261 aagtatgaac aagagcgaaa gagaacgttt gacagatatt gatgaaaaaa ctggtgtttt
     1321 cactggttct tatgctattc atccttttac aggtgaagaa attcctattt gggtagctga
     1381 ttacgtgtta atttcctatg gtactggtgc agtgatggct gtacctgccc atgataatag
     1441 agattatgct tttgctaagc aatttggatt acctattaag gaagtaatta aaggtgggaa
     1501 tatagcaaaa gaagcttatg aagatttaaa aggtgaatgt gtgaatagcg attttatcaa
     1561 tggattaaaa cctagcgatg caattaaaaa aataatagaa gaagttgaaa aacgtggtat
     1621 cggtactagg aaagttaatt atcgtttacg tgatgctatt tttagtcgtc agagatattg
     1681 gggagagcct ttcccgattt attataaaga tggaattcct tacacattag atgaatctga
     1741 attaccttta gaattgcctc tcgtagattc ttacttgcct accgaagatg gtgaaccacc
     1801 attagcacgt gctaaaaatt ggaaaactaa agacggatat ccattagaaa cttgtactat
     1861 gcctggtttt gctggttcta gtgcctacta tcttagatat atggatccta ataatgacaa
     1921 cgaactagtt tctaaaaaag cagtagaata ttggcgaaat gttgacctct acattggtgg
     1981 atcagaacac gctacaggac atctgattta tgctagattt tggaacaaat ttctctatga
     2041 tattagtgta gctgctgagg atgaaccttt ttttaaatta attaatcaag gtatgatact
     2101 cggtcgtagt aatttcgtat atcgtgctaa accattagat tttgctattt ataaattaaa
     2161 aaatgtacta cctgctatat ctgataatta tgaaattaat ttgcaagatg acattattat
     2221 aatcttttta caaaattata aaacttattt attaatagtt gaaaaagatg atatagataa
     2281 tgcaaaagct ttagccaata gtttagttac aaataattat aagattttaa ttataaatat
     2341 tacaaaaatt ttactaaaag ataatttaaa agaattttta gaaaaccaaa taaataactt
     2401 gcaagataaa gaaatcaaaa taatagacga agatatagat actcccccaa ttttcgtttc
     2461 taaaaacatc gaaggtagag aagaatatac atatcctatg catgtagata ttagtcttgt
     2521 gagaaatgat attttagata tagaagcttt taaaaaatgg caaccagaat ttgaacatgc
     2581 tcattttatt ttagaacttg ataaatatgt ttgcggatgg gaaatagaaa agatgtctaa
     2641 atctaaatac aatgtacaaa atccagatga tgtagttggt caatatggag cggatactct
     2701 gcgtctatac gaaatgtttc tcggtccagt agaacaaagt aaaccatggg acgttgctgg
     2761 tatagaaggt gtacatagat ttcttcgaaa attttggcgt ttattttttg atgataaaga
     2821 tgatttctat cttaatgata atgaacctaa cagagacgaa ttgcaaatat tacacaaaac
     2881 tttgaaaaaa gtacaggaag acatagaacg ttatgcatat aatactgcta tcagcacatt
     2941 tatggtagca gtgaataatt tacaggattt aaaatgtaat aatcgaaaaa ttttagaacc
     3001 attgataata gcattatctc catttgtacc atttataaca gaggaattat ggcatctagc
     3061 tgggcacaca gatagcatac ataaagccaa atttcctgat tatgatgaaa aatatactat
     3121 agataatacg tgcacttatg ctatatcatt taatggtaag actagattta ctttggatat
     3181 agacgcatca gcagatgaag aaactataaa agaaattatt ctcacacatc ctcaatctga
     3241 gaaatggctt aaaggtaaaa atcccaaacg tgtaatagta gtaaaaggta aaatagtgaa
     3301 tgtggttgtg taaatatatg atttgatgaa tttaatatca tatatttata tgaagttcat
     3361 taggcttaga tattattagc taatcctatg caactgtaaa taacaaaaat taaataatat
     3421 ctaaaaaatt aattataaaa atctaatgtt aatttctttt agcttgtaat tgcaaataaa
     3481 ttcttaggta tttatcatca gctggtatta tttgttcacc aattggatat attttattag
     3541 aaatagggtg ctttagtctc atattaataa tctgataatc aggcatatat ggattgtcat
     3601 ctatgatagg ccttttaata gctaacacag tagatttttg gtagttggtt aaaagtgatt
     3661 gagcatcttg aatgggtagt ttaatatcat accattgacc atttactttt atttgtaaaa
     3721 caaaattttt atcatcatat tggcctatac tatcaatagg aatctcaatt tcgtgatatt
     3781 gcatagcaaa tagcgtatcc ttttttttag cttgcagatt tcttatttta gtagcttcgg
     3841 catagcattt attcaatact gttaattgtc tggcggcaaa ttgttgtagt ctggcaatat
     3901 attgttgatc agtttctgtg atttttttag gagctgataa actatcaaat tcggtaatat
     3961 tagatttata acaagaatct aatataaact ggttcatatt gtatatgtac aattttttat
     4021 cactagtaat aatgcccatc catccaatag ggtaagctag attaatacct tctatagaac
     4081 tatttgcaat ttgagtttct gctatttttc ttctttcctg tatatttata atatgaatat
     4141 atcctaagtc atcagtaaaa gcagcataac gattatcaaa aaaacattct acattattaa
     4201 cgctaccacc ctttgattta aatgtagcta atttagtttt attttttaca tcccacaaaa
     4261 tagctgtatt gtcagttgaa ccacttatga gatatttgcc atctggagtg aaatcaatat
     4321 ctaaaactgc tcctttatga ccatttaaag taaacatctc atcagtttta agagaagtaa
     4381 aaactatctt gtggtcccag ccgccagata ccacccattc attaaatgga gatatagata
     4441 cacaggctac tgctgatgaa tttggtatat attttttata taaaactaat gaatctgccc
     4501 aatatactaa aacactctta tcttctgaac cagtaaccaa atatttgcca ttcttggata
     4561 ttgcaagatc atttatttta ttataatgtg gagttaatac tttctttaaa acattacctg
     4621 tactagcatc ccattgtatt atcattttgt caccaccagc tgtataaagc ctatttttat
     4681 catccattac aattgattta ataaagcctg tatgctcttt taatctctta ttaatatcca
     4741 tttttatcat atctaatatg cagaattctc catctatatt tgtgagatat gcatattgcc
     4801 cattattact caatactaca tcagtatata aattttctag tgtatttgat tgctttatta
     4861 atacctgact gaatagagaa tttataaata taattaaaat atttaataaa cataatttct
     4921 ttaacataac aataatatat tttattgttg taaaattaaa gaatattttt taattttgac
     4981 aaattttata aaaatttatc ctattatttt gaaacacaaa ttattttggt gtaatttata
     5041 ttcctttttt atttctgaaa taatgtaaac tatcgcccca ttcatcatag cctattctga
     5101 aaccagctat tcgaattctc gattctatac aacagatgtt atcatcagga tttttatcta
     5161 tatttggttt gtttttatag agtaaatatt catctctata ttttttaggt gttatatttg
     5221 gcattagcac attggctcca gcctgcaatg ctttttctct acccatagga tctatagctt
     5281 gcatagctgt ggtagcagct atattgatat ctggcatcat cagtcgcaga atagctatca
     5341 tttttaatgt tagactaaat cgttctttta ttggtaagag attattttta tatttatata
     5401 atggtgtatc tggatgctct atatatggtc ccattccaca catgtctata tctaaatttt
     5461 tcataaagat taaatcatca gctaaatgtt ctatggtttg atatggcaaa cctatcatta
     5521 tgcctgtgcc taattgatat ccgatttctt ttagatcaac taaacatcta tatcttttat
     5581 cgaaatcgtg tatgtcgtct tgtggatgta attttttgta taattcagga tgcgactctt
     5641 ctattctcaa tagatatcta tgagcgcctg cctcatacca tctttgatag gtttctttgc
     5701 tttgttcgcc cagagatagt gttataccca atttaccaat acttattctt ttaatttctt
     5761 taatgtattc tgtcatttta tctatgaatg ccgtactagt attttcccca ctttgtatca
     5821 ctatactgcc atatctatgt tttatcgcga aatttacagc ctcataaaat tcttcatctt
     5881 ttattgtata acgtgttaaa tttttattac cacttctgat accacaatac aaacaatttt
     5941 ttttgcaaat attagaatat tctattatac ctctgagata aactctatca cctcgatatc
     6001 taaggcgtat tttattggct tttttaaata aatttaactc atcgctacct cttagttgta
     6061 gcaattctat tatttcattt ttatctaaat ttgaaaaatc ttgtttgtct aaaatactct
     6121 tcattttttt gttaaaatct tttataaaat tataaatata tattgtttta atagcaagcg
     6181 aataataaat atcataaaac aaaatcaact tacaaaatta tcataataat cttaattttt
     6241 ctaaaaataa cgaatgaaaa acattaaatg ttttaaatat aaaatcttta atttctaatt
     6301 gcagcttaca attagacagt atgaatgtag attaaaattt ttatgtaaat ttgtatttta
     6361 taattttttt atttcttact cgttaattat atggttatta tgaaaaaata ctatattgta
     6421 cttcttagtc tattattttt aacaaatact tatgctcagc gtaatgaagt tggtataagt
     6481 ggtggaggta tgtattatat tggcgactta aatccatatt accattttta ttttactagg
     6541 ccacatatag gcttgtatta ccggtataat tacaaagatt atttaagcgt tagaattttt
     6601 ggtaattatg gtagtgtata tgcagcagat agtgttttaa attattatcc tgaaagaaat
     6661 ttacatttta aatctccaat tttagaaatt ggtgtagtgg gagaaataaa ttattataaa
     6721 tttcgtcctg gtgcccgatc tcatactttc accccttatt tatttttggg tataaattgg
     6781 ttttatttta atccaaaagc taaatatcaa gatgaatgga taaatttaca agctatgggc
     6841 actgaggggc aaggtactac actatatcca gaacgaaaac cttactcttt aaatagtttc
     6901 gctataccaa tgggtatagg tataaaaata gctctatcag aacattttat ttgccattta
     6961 gaatggggaa tgcgccgtac ttttactgat tatttagatg atgtcagcac tacataccca
     7021 gaccctctca tcttagctac ccagatagac ccaatagctc catatctatc agaccctaat
     7081 ctattagagt ataatgagtt aagaaataat aatatagata agtggcgtgg cgatccttct
     7141 actaaagatt ggtattcagt aataaatttg ggtttttctt ttagatttaa gaataaaagc
     7201 gggcattgtg agggaatgac acctcgatat agagacaaaa ttaaagatta tagagataat
     7261 ttgaaaagta aagaaaaaat tagattctga tatattatga aacaaaattt atttaataat
     7321 ctaccacttc atgtagccat catcatggat ggtaatggaa gatgggcatc aataagaggt
     7381 ttgcctcgag ttaaaggaca tgaagctggt attgatgctg ctaaaaaaat aattaataaa
     7441 gctggtgaga tgggcattaa atatttaact ttgtttgctt tttctataga aaattggggt
     7501 agaccacaag atgaggttat gtatttattt tcactttttg ataaggctat taatacctat
     7561 tctgatgaat taattgccaa tggtgtaaaa gtaaaattta tggggacttt gtctctattg
     7621 cctcctgata ttcaagaagg tgtatcatta ctagaaagtc aaactgctga caacaatcac
     7681 cttacacttg taatagctat gagctatggc tcccgacagg aaatagtaga tgccactaaa
     7741 gctatttctc aaaaaattct taatgatcag atgactattg atgatataaa cgttgatagt
     7801 ttcagacaat atctatattt acctgaatta cctgatcctg atctgataat tcgtacttct
     7861 ggagaaaaaa gattttctaa tttcttactt ttccagtctg cttatagtga attgtatttc
     7921 actgatactc tatggcccga ttttgatgaa agtgaatttg aagaagctat agctgacttt
     7981 tataatcgta aaagaagata tggtaaaatt tgaattttat gagattttta tattaaaatt
     8041 tttttttagc tacatgatat ggatacaata ttatattttt gcaagttgat ttatttttaa
     8101 attaacataa aaatttgttt atgtataaaa aaaccctcgc tttattttta ctgttattta
     8161 taataacttt tagctcgata gggcaaataa ttgatataga ttacagacta ccaaaagagt
     8221 atattattgg tggtgttaca gtatctggct tgcaaaatga agagtctaaa gatctgataa
     8281 tattgattag cgatttggtt ccaggcaaaa aaattactat accaggcgaa gatattcaaa
     8341 aagctattaa aaatttatac aagcaaggaa tgtttgaaac tattgaaata tctgtggtat
     8401 cacgtgctgc caatacgata tatttagata tttatatcga agagaaggat agagtatcaa
     8461 gttttgtaat agaaggtgtt aaaaaaaatg aagctaattc attaaaagaa aaaataaata
     8521 tccaaactgg tgatattatt acagagtttt tgatagaaaa tgtaaaaaat aaaatagaaa
     8581 aacattacat tgataaagga tttcataata taaaagtaaa aatcgaccaa ataccagaca
     8641 catcagcaaa aggtcgctct acactatata taaacataga caaaggtcca agagtaaaaa
     8701 tagaagaaat aaattttgaa ggtaatcaag tattaactga taaaaaacta aaaaaggcta
     8761 tgaaagatac aaaagaaaaa aatattttta atattttcaa atcttctaaa tttattttag
     8821 ctaattatga aaaagataaa gctaatatca taaataaata tgcagagttg ggatacaaag
     8881 atgctatcat tttaaaagat tctatatacc ccattaataa taaaagtctt aaaattgata
     8941 tttacctaga agagggcaaa aaatattatt ttagaaatat tacttgggta ggcaatacaa
     9001 aatattcgtc tgagacacta aatcaaatat tacaaattaa aaaaggcgat acatataacg
     9061 agaaattatt gcaaacaaac ctttttgcaa atcctaacgg gctggatgta gctgctctat
     9121 atatggacaa tggttattta ttttttcaaa tgaatccagt aattgtaaat atagaaaatg
     9181 atagcataga tatagaaata caaatatatg agggtaagca agctactata aacaaagtat
     9241 cagtaatagg aaatacagtt acaaatgata gagtgatctt aagagaagtg cgtagcaaac
     9301 ctggagatct ttttaaacgt tcagatatta ttcgcacaca gatggaacta gcacagatgc
     9361 gtttttttaa tcaagaagaa ttaaatgtaa atatgaatcc tgatccatca gctggcactg
     9421 tagatctaga atatgtagta gaagaaatat caactaatca aatagaactt aatggtggat
     9481 ggggacttgg cagatttgtc ggtacattgg gactcgtact taataatttt tcattaaaaa
     9541 atatatttaa aaaagatgct tggagaccta taccgtcagg tgatgggcaa attttagcta
     9601 taagagctca aaccaatgga caatattatc aagcttatag ttttagcttt atggagccat
     9661 ggttaggggg taaaaaacca aatgctttat ctatttctat ttatcattca ttagaatcta
     9721 atggtattag caaaaaaaag gatggcactt ataattccga aggcacttta ttacaaagac
     9781 aagaaatttc aatcaccggt gctagcgttg gattaggtct aaaactaaaa aaacctgacg
     9841 atttctctcg tctatttatt acagtgaatt atcaacatta taaattagat aaatattacc
     9901 aagttctcac atttcaaaat ggagttgcta ataattttaa tattggtgta tcttttagta
     9961 gaaattctac ggattcacct atttatccca aaagtggtag ttctatttat ttatcattaa
    10021 atataactcc gccatattca ttttttaaga aaaaaaattg gagtgaattg cctgattctg
    10081 tcaaatttag atggatagaa tatcacaagt ggaagataga attagaaaat tatacaaatg
    10141 tatatggtaa atgggtatta atggcaagag ctaaatttgg tttgatggga atgtacaata
    10201 gagatattgg gttatctcct tttgaaagat tttatttggg tggagatgga ctatctggtt
    10261 tttctctgga tggcagagaa gtaataggta tgcgaggtta tgctaatcaa gttttaactc
    10321 ctagagatcc aaagggcaat tatattggtg gcactattta tgcaaaatat actttagaaa
    10381 tgcgttatcc tttgtctatg aatccgatgg ctactatata tttgctcggc tttgtagaag
    10441 ctggtaatgc atgggtaaat ataaaagaat ttcaaccctt caacaccttg aaatctgctg
    10501 gtattggtgt gagactttat atgcctatgt ttggtttact tggtctagat tttggcatac
    10561 cttttgatga tacatttgca aataaaaaat atcaaggaca attccatttt tctattaatg
    10621 cgtcaattga ttaaaaaaaa tataattatg aaaaagttaa ttccattaat tttattagtt
    10681 ttttttagtg tgggactgat agcacagagc ccaactaaaa tagggtttgt agatacacaa
    10741 tacattttag aacaaatgcc tgcataccaa aatgctatta atcagttaga taatctgagt
    10801 gctcaatggc aacaagaact ggaacaaaaa tatgctgaaa tagatcaaat gtatagaaaa
    10861 tatcaagctg aagcatatct tttaccacaa aatgaaaaaa taaaaagaga aaacgaaatc
    10921 gtagaagctg aaaaagctgc aaaagaatta caaaagaaat acttcggacc agaaggtgag
    10981 ctatttcaaa gaagaaaagc ccttttgcaa cctattcaaa atcaacttta tgaagctatt
    11041 aatgattatg ccaaaaaaaa tcgtatgggt gctatttttg atatttctaa taatactact
    11101 ttgttgtttt atgataataa actcgatatt actaatgata ttaagatgaa tctcggatta
    11161 taagactttt gttttaataa attaattttt ttttaataaa ttttattaat tttgcaaatt
    11221 atatataaaa aataaataat atgaaaaaaa ttcccttagt aattattttg tctgtatttt
    11281 tgtcatttgc attaatggga caacaaacta aattaaaaat tggacatatt aatacaaatg
    11341 aattaatgca gttgatgcct ggtagagata ccgcagaaca agtattaact gtttacgcac
    11401 aaaacttaca aaaacaactt gaagcaatgg tcgaagaatt tcaaactaaa tacagcgact
    11461 acctagctaa tgaaaatcaa tttatagatg ctattaaaca agttaaacaa aaagagctgt
    11521 tagatttaca gactcgtata gaagattttc aagaagaagc tcaaaaatta ttagtcgcaa
    11581 aagagcaaga acttctgaat ccgctcatag ataaagctaa aaaagctata gaggaagtag
    11641 caaaagaaaa aggatatact tacattttag atacaggtac aggtgtcgta ttatattggg
    11701 aaagtggtga tgacataatg agtttcgtaa aagataaatt aggtatcaag taatcttttt
    11761 ttgtgtttga cattttatag attattatta aaataaaagt ttaataataa tttttttgaa
    11821 attaaaaaaa ttattatctt tgcaaaaata ttttggccca ttcgtctagt cggcctagga
    11881 cgccaggttt tcatcctgga aatcgggggt tcgaatcccc catgggtcac taattttaaa
    11941 aaaaaatcaa agtaaattat ggcacatcat aaatctgcaa agaaacgtat tcgttctaac
    12001 gaaaaaaaac gactttataa tcgatattac aataaaacca tgcgcactat ggtaaagaaa
    12061 ttttatgcaa ctgctgatcc ccaagaggca gctcagatgc taccatcttt atattctatc
    12121 attgacaaaa atgtgaaacg tggaataata cataaaaaca aaggtgcaaa tctaaaacat
    12181 aaaatcacaa aagaattaaa taaacgattg aattcagctg ctactacagc ataaatttat
    12241 tatattattg tacaattttt ttaattataa ttttaatata ttctaataat caaaaaaaat
    12301 attcttgttt actattttta gcactataaa aataaataat aaaattttta tttttgtaaa
    12361 aaatttttat
//
LOCUS       DAOPRN010000054        12206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_4381_length_12206_cov_106.758456, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000054 DAOPRN010000000
VERSION     DAOPRN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12206
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4381_length_12206_cov_106.758456"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1161)
                     /locus_tag="PKI65_06455"
     CDS             complement(205..1161)
                     /locus_tag="PKI65_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOB78117.1"
                     /translation="MEKDLIRLGIFYDGNYLLHVSNFYNYFHERKSRISLHGLHKYIR
                     YYVSNLENLDSRFITIIESHYFKSRLSVKEMGNKPNQLFFDRLFDDVLMMENVIAHYH
                     PVRNIQGIKMEKEIGINLALHAYDIANSGKIDILVLVACDGDYVSLIKKIHSLGIKVL
                     LVSWDFEYTDDQNYSRLTKTSQDLIEETTYHLALNEVIDNPNNKDNPFVEGLFVSKSD
                     RRYDYSTYENDFTNNDDMSNIESDKITHESTILSLKNGYGFIKYPPNNLFFHYQSVQD
                     TDFNELQVGDEVTFEIAKKENGEELAINVKSKKNISIDNLEF"
     gene            complement(1226..2266)
                     /gene="rlmN"
                     /locus_tag="PKI65_06460"
     CDS             complement(1226..2266)
                     /gene="rlmN"
                     /locus_tag="PKI65_06460"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOB78118.1"
                     /translation="MNKTKKLILGYDYDDLVNELKYLPDTRHYNIKQIYQWIYKKHVN
                     DFKLMTDLPNELRTKLNDKYTLTLAPPEQVSLAQDGTKKYLFKFDNDKYVETTVIKED
                     KRTTVCLSSQSGCKYGCKFCATGKMGFHGDLSTGEILWQLLAIDEIDKFTNIVFMGMG
                     EPLDNAESVFKVIKLLIDPNGWGLSNQRLTLSTIGIPDMLIHCIEKTKINIAWSLHSP
                     FDDERHQLMPVQNIYPLQTIIDILYEYKYYFSPHRKLTIEYLMLKDINISKAHADALA
                     KIANLINARVNLIPYNPHLHNEFLTPNYNEMINFQEKLKNKGVITTIRKSKGNEILAA
                     CGNLYTNKKKIH"
     gene            complement(2320..3648)
                     /locus_tag="PKI65_06465"
     CDS             complement(2320..3648)
                     /locus_tag="PKI65_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HOB78119.1"
                     /translation="MAKKYNRWFIKLINHFKLTHQLEDFETIYKEISQGVVFRGTNLW
                     ILACSIIVCSVGLNMNSTAVVIGAMLISPLLGPINGIGYSIAIYDLKLFRKALNNFLF
                     AVTTALIAATLYFLITPISTAQTELLARTSPTIYDVLIALFGGIAGIIAMSTKNKGTV
                     IPGVAIATALMPPLCTAGYGLATLQPNFTFGALYLFTINAVFIALASLLISKLLKIPI
                     HDDVPSARIKVINRYVSAIIILTLIPSIYLGHLLVQKEKFIEKTNKYVNNVRVINDKY
                     LLDYSIDPRKKIINLVYGGTTLSENDKKLITQRAKDFDLKADINIEQGFAIDQREYQE
                     KENLLLRISGLEAELNKRKNQLDSIKNRPLLAQALLNELTIINDTISSCAIADTYIFK
                     TNNITDTTTIVIIGTKYENISSAGQNKIINWLKARLNSTKIQVFFIKSYH"
     gene            complement(3903..3989)
                     /locus_tag="PKI65_06470"
     tRNA            complement(3903..3989)
                     /locus_tag="PKI65_06470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3953..3955),aa:Ser,seq:gct)
     gene            complement(4064..5128)
                     /locus_tag="PKI65_06475"
     CDS             complement(4064..5128)
                     /locus_tag="PKI65_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOB78120.1"
                     /translation="MAKQQENKKNEKTSKDTKKKVDEVKKDQNTKTNENKNIKDNKKT
                     TKTKSSTTSKSTTKTTKPKETKTTKKENLTKTNEKEITKQQDDILATSIKVIEDEGSE
                     TLEQIIIDKLKALYRLQYIHSQLDKIRIIRGELPAEVQDMEDEIEGLKIRMGKIKDEI
                     QKLEENIKYHKIKISELSENIKHRKEQQYQVRNNKEYEALTKEIENLELDIQLAEKRI
                     RENQEKINQKKEQLKEAQSLIDEKIAILSDKKAELDEIIKETEEEEKRLNTILKKAMK
                     DVDERYLKAYTRIRNGVSNGLAVVGIVRNACGGCFNNIPPQRQLEIKLHKKILICEYC
                     GRILVDDQIIKEVENEFVNK"
     gene            complement(5140..6237)
                     /locus_tag="PKI65_06480"
     CDS             complement(5140..6237)
                     /locus_tag="PKI65_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOB78121.1"
                     /translation="MKLNDIIKIIEASYPLYIQEPYDNSGLIIGDKEANINGILIALD
                     LNNDVIKEAIKNNCNLIITHHPFIFNNIKKLEAGDELFDKLNLIFKNEINVYSSHTPM
                     DKSFNGINIALAERLNLKNIQILYPENNLLCKLVTFCPLNYADKVRESLFNAGAGVIG
                     NYDCCSYNVQGLGSFRAGDDTNPFVGKKGEIHYEPETRIETIFPKWLTNDIISALLYS
                     HPYEEVAYDIYQLENTHPKVGLGAVGNLNNEMEVYDFLTFVKDIFKLPTLRYSYVENI
                     KKIHKVALCSGSGADILPIAINQKADAYLTADIKYHDFQKAHNKILLLDIGHYESEII
                     FVEKMYDYLSKKIPNFAIQKSENSYSFINFF"
     gene            complement(6363..7625)
                     /locus_tag="PKI65_06485"
     CDS             complement(6363..7625)
                     /locus_tag="PKI65_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOB78122.1"
                     /translation="MRIKKIISFFLIIISLNLYSQKVLIPMDNAQTNHLKAYGLVYFM
                     LQNEFEVEWLLNYRGGSFLMPYGKPLIQECVLRGVNYEVITIPQATAIYEYISDPSIN
                     CDIIKLTKAPRIAVYAPPTNLPWDDAVTLVLTYAEIPYDQIYDKEVLQGILPMYDWLH
                     IHHEDFTGQYGKFWAQYRNTEWYQKDQQYAEQLARSLGFSKVSQLKLAVAKKIRDYIA
                     GGGYLFAMCSAPESMDIALAAEGVDICDIPFDGDPPDPRAQEKLDYSKTLAFTDFKIY
                     TNPYIYAKSDIDVTMTRPQIREEDDYFALFEFSAKWDPIPTMLCQNHKRIIKGFMGQS
                     TAFNKNKIKKDVLIMGENKSLNEAKYIHGEYGRGTFTFYGGHDPEDYRHFVYDPPTDL
                     NLFKNSPGYRLILNNVLFPAAQRKKLKT"
     gene            7800..8108
                     /locus_tag="PKI65_06490"
     CDS             7800..8108
                     /locus_tag="PKI65_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78123.1"
                     /translation="MNIKELVKKTYYMGLGAIASVQDKAEEIANQLIERGELKEKDKP
                     EFIKELVEEGEKLKSNLDEKIQSAVDAAIKKLNIPTKDDIDELNKKIDAILNELNKEQ
                     "
     gene            8130..9779
                     /locus_tag="PKI65_06495"
     CDS             8130..9779
                     /locus_tag="PKI65_06495"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOB78124.1"
                     /translation="MAINEFFFRKKSKHKLRRSREIIRVLIKYGFYRPPKKKISLKKT
                     KKASIHLSRPERLRLALEELGPTFIKLGQILSVRPDLLPLDYIKELEKLQDQVCCSEE
                     VQIDEIFLKNFGKSINDIFILIESTPFASASLSQVYKAQLKNGDWVAIKVQKPNVKEI
                     IKTDLNILTDLANLSKIIVRDDWIFQPPLIVNEIKRSLTKEINFVLEGLNYERFSENF
                     KNVDYVKLPKVFWEYTNTEILTMEYIDAIKIKELFACSSDKYDKKKIAERGARIALKQ
                     IFVDGFFHADPHPANVLVKPPATIVMIDVGMVGAIDTNTRKILLNLLNAFIDEDMDAV
                     ITCFKKLNIIKHDDHKFNEVAFRRDLYDMIDKYFGMPLKYFDLREISQELFRTIYKYN
                     LSLPANLTLMLKAIGTVENTGKQLDPYFDIYSTIESFIKEDFLRKLSPSYIFNKSKKT
                     LAQSVDILENLPNDVFNFVNKIKDLDGIKIKIDKDTQDKITKSIKNSVLLVSLSIIMA
                     AVIISASVFIIYNQKNWLIILGLFMIFLIVLVFLYKSIFKN"
     gene            complement(10054..10722)
                     /locus_tag="PKI65_06500"
     CDS             complement(10054..10722)
                     /locus_tag="PKI65_06500"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOB78125.1"
                     /translation="MKRVNFILLLASAVLMIFISSCTMQKRLYTSGYHIEFKNGNPTT
                     VKKNVDNDEVKNNKNASSIATATTIKESKSQTNDINDVIINDAIINNPTSTNTNENIV
                     ASTTNDPVVYTSQKTSSWYNASAVPKTNINTDKKANVKQTNTNNENANAQATKSAGKS
                     WIAALLLCIFLGTLGIHRFYLGYTWQGIVQLLTLGGLGIWTLIDLIRIITRDLQPKSG
                     HYTD"
     gene            complement(11171..11908)
                     /gene="rlmB"
                     /locus_tag="PKI65_06505"
     CDS             complement(11171..11908)
                     /gene="rlmB"
                     /locus_tag="PKI65_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOB78126.1"
                     /translation="MSDKLYIAGTRAVLEALESNVAINKIFIIKQKQEYREIYKHIFS
                     EAKKLGIPVQYVPIEKLNQLYKGNHQGIIAETSPVQYYELTSFLEDLKNEKPLLLLLD
                     RITDVRNFGAIARTSLAAGVDGIIIPQSGNVKITDDAIKASAGALFKIPVIRVIHLKD
                     AIYQLKTYNYSIFAATEKAKKTIYEADFKKPIALILGNEEKGIQPTLLKLCDDIIKIP
                     IIGNIDSLNVSVSAGIIIYEILRQRFL"
BASE COUNT         4093 a         1714 c         1658 g         4741 t
ORIGIN      
        1 aatttaataa attttctgat attaatcctg tataaatttt aacagattaa atattttcat
       61 ttaccccctg atttgaaata gttttatttt gcctattata tgtcgcttag ttaatcttta
      121 aaaaatgata ataagatgga taatcaatca aaaaatcgca aacaagaact agtttttgtc
      181 taattaattt caaaatagta atgttcaaaa ttctaaatta tcaattgaaa tatttttttt
      241 agatttcaca ttgattgcta attcttctcc attttctttt ttagctattt cgaaggttac
      301 ttcatctcct acttgcaatt cattaaaatc tgtatcttgc actgattgat agtgaaaaaa
      361 caaattatta ggtggatatt ttataaaacc ataaccattt tttagagaca aaatagtact
      421 ttcatgagtt attttatcac tttctatatt tgacatatca tcattattag taaaatcatt
      481 ttcataagta gaataatcgt aacgtcgatc agatttagaa acaaataaac cttctacaaa
      541 tgggttatcc ttattattag gattatcaat tacttcattt aaagctaaat gataggtggt
      601 ttcttctatt agatcttggg aggtttttgt taatcttgag taattttgat catcagtata
      661 ttcaaaatcc catgagacaa gtaaaacttt aatccccaaa ctatgtatct ttttaattag
      721 agaaacgtaa tctccatcac aagctactaa gacaagaata tctattttac cgctgttagc
      781 tatatcatat gcgtgtaaag ccaaattgat acctatttct ttttccattt taattccttg
      841 gatatttcgt acaggatgat aatgagctat cacgttttcc atcattaaga catcatcaaa
      901 taagcgatca aagaaaagtt gatttggttt gtttcccatt tcttttacac ttagtctaga
      961 tttgaaatag tgactttcta ttatggttat aaatctacta tctaagtttt ctaaatttga
     1021 tacataatat ctaatatatt tatgtaggcc atgcaaacta attctagact ttctctcatg
     1081 aaaataatta taaaagttac ttacatgtaa taaataattc ccatcataaa aaattcctaa
     1141 tctaattaaa tctttttcca tatttctata ttttgtattt ttttatttgc aaatgtaaat
     1201 tattttttta aaaaaaagat ttttatcaat gtattttctt tttatttgta tacaagttac
     1261 cacaggctgc tagtatttca ttacccttag atttgcgtat agtggttatt acccctttat
     1321 tttttaattt ttcttgaaaa tttatcattt cattataatt aggagttaaa aattcattgt
     1381 gtaaatgtgg attgtaaggt attaaattaa ctcttgcatt tattaaattt gctattttgg
     1441 ctaatgcatc tgcgtgagct ttagaaatat ttatatcttt aagcattaga tattcgattg
     1501 tcaatttcct gtgggggcta aaataatatt tatattcata taagatgtca atgatagttt
     1561 gcaaaggata aatattttgt acaggcatca actgatgtct ctcatcatca aaaggactgt
     1621 ggagactcca tgcaatattt attttggttt tttctatgca atgtatgagc atgtccggta
     1681 ttccaattgt agataaagtt aatctttgat tgcttaaccc ccaaccatta ggatctatta
     1741 gcagcttaat aaccttaaat actgattcag cattatctaa tggttccccc attcccataa
     1801 aaacaatatt agtgaatttg tctatctcat ctatagctaa cagttgccaa aggatttcac
     1861 ctgtagagag atcgccatga aaccccattt tacctgtagc gcaaaactta catccatatt
     1921 tgcatccaga ttgcgaagat agacatacag tggtgcgttt atcctccttt attacggttg
     1981 tctcaacata tttatcatta tcaaatttaa ataaatattt ttttgtacca tcttgagcta
     2041 aactgacttg ctctggtgga gctaaagtca atgtatattt atcatttagt tttgttctta
     2101 gctcattagg taaatcagtc attagtttaa aatcatttac gtgtttctta taaatccatt
     2161 gatatatttg tttgatatta taatggcgag tatctggtaa atatttcaat tcgtttacta
     2221 aatcatcgta gtcgtaccct aagattaatt tttttgtctt attcatggtt ttatctgtaa
     2281 attaaatgta atagctgtta aaataattta attattgggt tagtgataag attttataaa
     2341 aaatacttga atttttgtac tatttagtct ggcttttaac caattaatta ttttattttg
     2401 tcccgcagac gaaatatttt catatttagt tccaataatt actattgtgg ttgtgtcagt
     2461 aatattatta gttttgaata tatatgtatc agctatagca caagatgaga tggtatcatt
     2521 gattatagtt agttcattta ataatgcttg tgctaaaaga ggtctatttt taatgctatc
     2581 tagctggttt ttacgtttat ttaattcagc ttctaatcca ctaatacgaa gcaggagatt
     2641 ttctttttct tgatattctc tctgatcaat agcaaatcct tgctctatat ttatatctgc
     2701 ttttaaatca aaatctttgg ctctttgagt tattaatttt ttatcatttt cactaagtgt
     2761 agtgccacca tatacgagat taattatttt ttttcgtgga tctattgaat aatctaaaag
     2821 atacttatca tttattactc taacattatt aacatatttg ttggtttttt caataaattt
     2881 ttctttttgt actagaagat gtcccagata gatactcggg attagcgtta atatgataat
     2941 agcactaacg tatctattaa tgactttgat tctagcagat ggcacatcat catgaatagg
     3001 tatttttaat aatttagaaa taagtaatga tgcaagagct ataaatactg cattaatagt
     3061 aaaaagatac agagcaccaa atgtaaaatt gggttgtagg gtagctaatc cgtagcctgc
     3121 tgtacagaga ggtggcatca gagctgtagc tattgccact cctggtatca ctgtgccttt
     3181 attttttgta ctcatagcta ttatgcctgc tataccacca aagagagcaa ttaatacatc
     3241 ataaatagta ggactagtac gcgctagtaa ttctgtttgt gctgttgata taggagtgat
     3301 caaaaaatat aaagtggcag ctatcagagc tgttgtaaca gcaaataaaa aattattgag
     3361 tgcttttcta aataatttta aatcgtaaat tgcaatacta tatcctatac cattaatagg
     3421 gcctaatagc ggtgatatta acatggctcc tattactact gctgtgctat tcatgtttaa
     3481 ccctaccgaa catactatta tcgaacaagc taaaatccat agatttgtcc ctctgaatac
     3541 tacaccttgt gatatttctt tatatatggt ttcgaaatct tctaactgat gtgtgagttt
     3601 aaaatgattt attaatttta taaaccatct attatatttt ttagccatga ttaattattt
     3661 ttacaaaaat acatagtatt tttatgattt tgtgaatatt tttatatcat gttgttaatg
     3721 taattaataa aaacattaaa aaattgtagt taacaaacaa aaagaaattt taaattttat
     3781 aaaaatttca aattctaact agcatatgtt atagcctcct gtcttctcac tgattagcta
     3841 tccttagttt ttgagatttt tgatttagat ttttattcat gaattttaat gaaatataat
     3901 agcggagaga gggggattcg aacccccgat acccttgcga gtatcgcagt ttagcaaact
     3961 gctggtttca gccactcacc catctctcct tttaaatttt tttgcaaaat tacaataaaa
     4021 aaataaacta aaaaaatttt acaaataaaa caagaagttt aaattattta ttaacaaatt
     4081 cattttctac ttctttgatt atctggtcat ctacaagtat tcgtccgcaa tattcgcaga
     4141 taagtatttt tttatgtaat ttaatttcta attgacgttg tggtgggata ttattaaaac
     4201 aacctccaca agcatttctt actattccga cgactgccaa tccattagat actccattac
     4261 gaatgcgagt ataagctttt aggtaacgtt cgtctacatc tttcattgct tttttgagaa
     4321 tagtattaag gcgtttttct tcttcttcag tttctttaat tatttcgtct aattcagctt
     4381 ttttatcact caaaatagca attttttcat ctattaatga ctgtgcttcc tttaattgtt
     4441 cttttttttg atttattttt tcttgatttt ctctaattcg tttctcagct aattgtatat
     4501 ctaattctaa attttcaatt tcttttgtta atgcttcata ttctttatta ttgcgtactt
     4561 gatattgttg ctcttttcta tgtttaatat tctcacttag ctcagaaatc tttattttat
     4621 ggtatttaat attttcttcc aatttttgta tctcatcttt tatttttccc attctaattt
     4681 tcaagccttc tatttcatct tccatatctt gtacttcggc tggtaattca ccacgaataa
     4741 ttcttatttt gtcgagctgt gaatgtatgt attgtaatct ataaagtgct tttagtttat
     4801 ctataattat ctgctctagt gtttcagatc cttcatcttc aattaccttt atagatgtag
     4861 ctaaaatatc atcttgttgt ttagtgattt ctttttcatt tgtttttgtt aaattttctt
     4921 ttttagtagt tttagtttct tttggtttag tagttttagt tgtacttttt gatgttgtac
     4981 tagatttagt ttttgtagtt tttttattat cttttatatt tttattttcg tttgttttag
     5041 tattttgatc ttttttaacc tcatctacct tttttttagt atcttttgag gttttttcat
     5101 tttttttatt ttcttgttgc tttgccataa gtatttatat taaaaaaaat taataaatga
     5161 atatgaattt tctgattttt gaattgcaaa gttaggaatt ttttttgata aataatcata
     5221 catcttttct acaaaaataa tttctgattc ataatgtcca atatctaaca acaaaatttt
     5281 attatgagct ttttgaaaat catgatattt gatatcagct gttagataag catctgcctt
     5341 ttgattaata gcaataggca aaatatctgc acctgatcca ctacataatg caactttatg
     5401 tatttttttt atattctcta catagctata tctgagtgta ggtaatttaa aaatatcttt
     5461 tacaaaagtt aaaaaatcat atacttccat ttcgttattc aagtttccaa cggcccccaa
     5521 accaacttta ggatgcgtat tttctaattg ataaatatca tatgccacct cttcgtaagg
     5581 atgactatat aataatgcag agataatatc atttgtgagc catttaggaa atatagtctc
     5641 tattcttgtt tctggttcat aatgtatttc tcccttttta cccacaaaag gatttgtatc
     5701 gtcacctgct ctgaaactgc ctaaaccttg cacattgtag ctacaacaat cataattacc
     5761 tataactcca gctcccgcat taaataaaga ttctcttacc ttgtcagcat aattaagtgg
     5821 acaaaaagta actaacttac aaagtaaatt attttcagga tatagtattt gaatattttt
     5881 taaatttaat ctttctgcta aagctatatt tattccatta aaagatttgt ccataggggt
     5941 atgagaggag taaacattta tttcattttt aaaaattaaa ttaagcttat caaaaagttc
     6001 atcgccagct tctaattttt ttatattatt aaaaatgaag ggatgatgag ttattataag
     6061 gttgcaatta ttttttatag cttcttttat tacatcatta ttcaaatcca gggcaatcaa
     6121 tattccatta atgtttgcct ctttatcacc tattataagt cccgaattat catatggctc
     6181 ttgaatatag agtggatagg atgcttcgat tattttaata atatcattta gtttcatacc
     6241 atattttatt tttttattaa caaatatata taaaaaaatc agatatcata tttgtatcta
     6301 caaagttatt ttggttgcca attaattttt tgcaaattat agcatgttat ttatgctttt
     6361 tattatgttt ttaatttttt tctttgtgct gcagggaaaa gtacattatt aagtattaat
     6421 cggtaaccag gagaattttt aaaaaggtta agatcagtag gtggatcata aacaaaatgg
     6481 cgataatctt cggggtcatg tccaccataa aaagtaaaag tacccctccc atattcgcca
     6541 tgaatatatt tagcttcatt gagagatttg ttttctccca ttatcaatac atctttttta
     6601 attttatttt tattaaaagc agttgactgt cccatgaaac ctttgattat tcttttatga
     6661 ttctgacata gcatagtagg gatagggtcc catttagcag aaaattcgaa taatgcaaaa
     6721 taatcatcct cttcccttat ttgtggacgt gtcatagtta catcaatatc agatttagca
     6781 taaatgtagg gatttgtata aattttaaaa tcagtaaaag ctaaggtttt tgaataatct
     6841 aatttttctt gtgcacgagg atcgggcgga tcaccatcaa aaggtatatc acaaatatct
     6901 actccttctg cagctaatgc tatatccata ctttcaggtg cactacacat agcaaataaa
     6961 tagccacctc ctgctatata atctcttatt ttcttggcaa cagctaattt taattgagaa
     7021 acttttgaaa aaccaagaga tcttgctaat tgttcggcat attgttgatc tttttggtac
     7081 cattcagtgt ttctatattg agcccaaaat ttgccatatt gccctgtaaa atcttcgtga
     7141 tgtatatgta accaatcgta cattggcaaa ataccttgca aaacttcttt gtcataaatc
     7201 tgatcgtaag gtatctctgc ataagttaaa actaaagtga cagcatcatc ccaaggcaaa
     7261 tttgtgggtg gtgcatatac agctatgcga ggcgcttttg ttaatttaat aatatcacaa
     7321 ttgattgacg gatcagatat atattcgtaa atagctgtag cttgaggtat agtaatcact
     7381 tcataattga cccctcttaa aacacattct tgaattaatg gcttaccata tggcatcaaa
     7441 aaactccccc ccctatagtt tagtagccac tctacctcga attcattctg aagcataaag
     7501 tatacaagtc cataagcttt taaatgatta gtctgagcat tgtccatagg tattaaaacc
     7561 ttttgactat acaaattcaa acttattatt atcaaaaaaa atgatattat tttttttatt
     7621 ctcatatctt attttaatct ttttttttaa tgtaaaatta ataaaatttt ttaaatatgg
     7681 tgtgcaatta cattattaat ttttgagatt taatgttgaa attattattt agattaatat
     7741 ataaaaaaat tatgtaaacc ccgaaatatt tattaatttt gtaaaaataa ttacaaaaaa
     7801 tgaatataaa agaattagta aaaaaaacct attatatggg tttaggagca atagcttcag
     7861 tgcaagataa agcagaagag atagctaatc aattgataga gcgaggagag cttaaagaaa
     7921 aagacaaacc agaatttata aaagaattgg tagaagaagg tgaaaaacta aagtcaaatt
     7981 tagatgaaaa aatacaatcg gctgtggatg ctgctattaa aaagctaaat atacctacaa
     8041 aagatgatat agatgagtta aataaaaaaa tcgatgcaat tctaaatgaa cttaataaag
     8101 aacaataatc tcaactaaaa catagaggtt tggctattaa tgagtttttt tttcgaaaaa
     8161 agagtaaaca taagctacgt cgcagtagag aaatcattag agtacttatt aaatatggtt
     8221 tttatagacc tcctaagaag aaaatttctc taaaaaaaac taaaaaagca tctattcatt
     8281 tatcacgtcc tgagcgattg agattagctt tagaagagct tggtcctact tttataaaat
     8341 tggggcaaat actcagtgtc aggccggatc ttttgccatt agattatata aaagaactag
     8401 aaaaactaca agatcaagtt tgctgctctg aagaggtgca aatagatgaa atttttctta
     8461 aaaattttgg aaaatctata aatgatattt tcattttaat agaaagcaca ccttttgctt
     8521 ctgcatctct cagccaagta tataaagcac agttaaaaaa tggtgattgg gtagctatta
     8581 aagtacaaaa acccaatgta aaggaaataa ttaaaactga tttaaatatt ttaactgatt
     8641 tggctaatct atctaaaata atagtcagag atgattggat ttttcaacca ccattgatag
     8701 taaatgaaat aaaacgttct cttacaaaag agataaattt tgtgttagaa gggcttaatt
     8761 atgagagatt tagtgaaaat ttcaaaaatg tagattatgt aaaattgcca aaagtatttt
     8821 gggaatatac taatactgaa attttgacta tggaatatat cgatgctatt aagataaaag
     8881 aattatttgc ttgctcaagt gataaatacg ataaaaagaa aattgctgaa cgtggtgcta
     8941 gaatagcttt aaaacaaatt tttgtagatg gtttttttca tgctgatccg catccagcta
     9001 atgtattagt aaaaccacca gctactatag taatgataga tgtgggtatg gtaggagcca
     9061 tagatacaaa tactagaaaa atattattga atctattaaa tgctttcatt gatgaagaca
     9121 tggacgctgt tatcacttgt tttaaaaaat taaatattat taaacacgat gatcataaat
     9181 ttaatgaagt tgctttcaga agagatctat acgatatgat agataaatat tttggtatgc
     9241 cactgaaata ttttgatcta agagaaatat ctcaagaatt atttagaact atatataaat
     9301 ataatctatc tttacctgct aacctaacgc ttatgctaaa ggctattggc acagtagaaa
     9361 atactggcaa gcaattagac ccttattttg atatttattc cactattgaa tcttttataa
     9421 aagaagattt tttaagaaaa ttatctcctt cttatatttt caataaatct aaaaaaacct
     9481 tagctcaatc agtagatata ttagaaaatt tacctaacga tgtttttaat tttgtaaata
     9541 aaataaaaga tctggatggt ataaaaataa aaatagataa agatacacag gataaaataa
     9601 caaaatctat taaaaactct gttcttctag tatcacttag tatcatcatg gcagcagtta
     9661 tcatcagtgc ctcagttttt attatctata atcaaaagaa ttggttaatt atcttaggtt
     9721 tgtttatgat ttttttaatt gttcttgtgt ttttgtataa atcgattttt aaaaattaaa
     9781 aatttaagta tttttttaaa aaaaaagctt tactcttaat gaataaaatt atttttatag
     9841 attaagctca ttggtcatat gatagtagat aagtaagatt ttatcccaaa tagatttcga
     9901 aaaaaatcaa aaattttagt tttataaaaa cctattgtta ttcaataatg atgtaaatgg
     9961 atagagataa tacacaaaaa attgttaaaa aataataata aaccagaggt gacacctctg
    10021 gtttattatt attaactaaa ataaatcatt attttaatca gtatagtgcc cactctttgg
    10081 ttgtagatct ctagtaataa ttctaataag atctattagt gtccagatac caagaccacc
    10141 tagagtaagc aattgaacta taccctgcca tgtgtagcca agatagaagc ggtgaatacc
    10201 taatgtacct aaaaatatac aaagtaatag tgcagctatc cagcttttac cagcagattt
    10261 agtagcttga gcatttgcat tttcattgtt tgtattagtt tgtttcacat tagctttttt
    10321 gtcagtatta atattagttt tagggacagc tgaagcgtta taccatgaac ttgttttttg
    10381 agacgtataa actacaggat cattagtagt agatgctaca atattttcat ttgtatttgt
    10441 agaagtagga ttatttataa tagcgtcatt tattataaca tcatttatat cattagtttg
    10501 agatttagat tcttttatag tcgttgcagt agctattgag ctggcatttt tattgttttt
    10561 cacctcatca ttatcgacat ttttctttac agtagtgggg ttaccatttt taaattctat
    10621 atggtatcca gaggtatata acctcttttg cattgtgcag gagctaatga atatcattaa
    10681 tactgcactt gctaatagta aaataaaatt tactcttttc atattttttt gtttttattt
    10741 tttcccctac tcatatggct tttcgggaac cgccccgttt ttttagtggt ttctagactc
    10801 cacgttatca aactttatta aagaaatttg tatattttct tttttattaa caatcaaaaa
    10861 gctatggggg acgtttcttt atataattaa aaagatgtga tttatcaata tatatatatg
    10921 tatatatggc ttttgtcgta aaattacgct tgttggtaga aatgattacc tcatcatcaa
    10981 tcataaaata ataaatatat cagttacaaa aatatatatt tttaatgaat aaacaaatat
    11041 tttttatttt aaaaatattt tttgaagttt tacataaatt ctaaatgatg gagaatgaca
    11101 atatgccttt atgatgattt tttgtgaata atcatatgca ggtaaacaaa ttaaacttat
    11161 ttaaaaataa ttataaaaat ctttgtctta agatttcata aataattatt cctgcactta
    11221 cagatacatt gagagaatcg atattgccga tgattggtat ttttataata tcatcacaaa
    11281 gttttaataa tgttggttga atgccttttt cttcattacc tagtattaga gcaataggtt
    11341 ttttaaaatc tgcttcataa atagtttttt tagctttttc tgtagcagcg aaaatagaat
    11401 aattatatgt ttttaattga taaatagcat cctttaggtg tataacacga ataacaggta
    11461 ttttaaataa agcaccagca gatgctttga tagcatcatc agtgattttt acattaccac
    11521 tctgaggtat tatgatacca tcaacacctg cagctaaaga agtgcgagct atagctccga
    11581 aatttctaac atctgttata cgatctaata ataaaagtaa aggtttttca ttttttaaat
    11641 cttctaaaaa acttgtcaat tcatagtatt gtaccggaga agtctctgct ataattcctt
    11701 gatgattgcc tttgtataat tgattcaatt tttctatagg tacatattgt acagggatac
    11761 ctagcttttt agcttctgaa aaaatatgtt tataaatttc tctatactct tgtttttgct
    11821 taattataaa aattttattt atggctacat tactctcgag ggcttctaat acagcacgtg
    11881 tgccagctat ataaagttta tcagacattt ttttttacaa aaataattta ttattataat
    11941 atctaacata tgttttaaat atttaaaaca taatgctcat tacaagtttt aacttataaa
    12001 tatgaattaa cttcaatgat ctcagtgcaa gtctgaaaca aatcacaata ataaaaatat
    12061 caaacatatt gtgtttattt cattggcttt ttatttagaa tttagtattt cgcattttat
    12121 atgtttgaac tcaatttagt atatggggaa ggtagaagtt tgagaaatta gaaccgtgga
    12181 gtaatgaatt ttactctaat aataat
//
LOCUS       DAOPRN010000055        12129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_4436_length_12129_cov_105.741594, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000055 DAOPRN010000000
VERSION     DAOPRN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4436_length_12129_cov_105.741594"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(412..1503)
                     /gene="lpxB"
                     /locus_tag="PKI65_06510"
     CDS             complement(412..1503)
                     /gene="lpxB"
                     /locus_tag="PKI65_06510"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOB78127.1"
                     /translation="MKYFILSGEQSGDLHGSNLAKAIFAIDKEAIIYGWGGELMKNAG
                     VNITKPITELAFMGFVEVLINLKKILKNFKEFKETIIELKPDKIILIDFPGFNLRVAK
                     WLRKNNFKIYYYILPQVWAWNRSRAKKIKKYIDVPISILPFEKKFYKKFSTDVHYVGH
                     PLLDHIDTFKCSSLNNIHSNKPIIALLPGSRIQEIKLLLPIMMKAVKPYEKEFQIYVA
                     GLSFIDKNIYYNSGVTTDQLLINDTYNLLQSSYVAIVTSGTATLEAALFDVPLVVCYK
                     ANWISYLIARIMARISFISLVNLIYGEEIVKELIQEKCSAENIRRELDKILFDLTYRQ
                     QMLDHFDELKKILGDSGASKKAAEIIVNN"
     gene            complement(1612..3756)
                     /gene="pnp"
                     /locus_tag="PKI65_06515"
     CDS             complement(1612..3756)
                     /gene="pnp"
                     /locus_tag="PKI65_06515"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOB78128.1"
                     /translation="MQEIKEIINLPNGQIIEISTGKLARQADGAVVLKCGDTMLLATA
                     VSKDEASPEVDFMPLTVEYQEKFAAAGRFPGGFLKRESRPSEYEILIARLVDRALRPI
                     FPEDYHAETQVLIYLISADKNYPPDALTALAASSALTISDIPFEQPISEVRVVKINGE
                     FIVNPSITEIESADLDLMVGGTLTDVNMVEGECREVDEDTLLEALKIAHEYIKIEIEG
                     QIRLIEKAGKPKRKYEHEVNNEEIRQKLEEYCYPRLLEISRQHIKSKKERQQQFEAIK
                     EDFLNQYSDEEREEIYPMVNRYFHEVHKRAVRQMVLDERCRLDGRRLDEIRPIWCEVD
                     YLPSAHGSAIFTRGETQSLTTVTLGTKLDEQLIDGAVYEGTSNFLLHYNFPPFSTGEV
                     KPIRLGRREIGHGNLALRALKNMIIRDEDNPYTIRVVSDILESNGSSSMATVCAGSLA
                     LMDAGVKMHKSVSGIAMGLIMDEKSGNYAILSDILGDEDHLGDMDFKVAGTTDGITAC
                     QMDIKIKGLSFQILSEALQQAKKGRLHILDIMNQTISISRSDYKPNVPRYAKLTIPRE
                     FMGAVIGVGGKVIQELQRETDSTIVIEEAGDMAIVDIFSTNKEGLEKAIKKIKAITTV
                     PQIGDVYDGIVKSIQSYGAFVEILPGKEGLLHISEIDNKRVNDVNDYYKIGDHVQVKI
                     LDIDAKTGKFKLSHKALLNKDNTNNNKNRNKN"
     gene            complement(4024..4293)
                     /gene="rpsO"
                     /locus_tag="PKI65_06520"
     CDS             complement(4024..4293)
                     /gene="rpsO"
                     /locus_tag="PKI65_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOB78129.1"
                     /translation="MYLTPEKTRDLAIKFGGSENNTGDTAVQIAIFTERILYLTEYLK
                     ENKKDFSAKRSLLKMVNKRKRLLKYLKRKDYDRYLEVTKQLNIRK"
     gene            4397..5128
                     /gene="gldF"
                     /locus_tag="PKI65_06525"
     CDS             4397..5128
                     /gene="gldF"
                     /locus_tag="PKI65_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOB78130.1"
                     /translation="MWALYKKELSDFFTSLVGYIVICIFLLATGLVLWLFPNEFNITL
                     GGYAVLDGLFILAPFVFLFLLPAITMRMFAEEYNTGTIELLFTFPISRWQIIFSKFLA
                     GFTIFLLSLLPTLIYFYSVYQLALPVGNVDIGGVAGSYIGLILLGMAFLALGIYSSSI
                     ANNQLIAFLIAFVLSLFFYIGFEYISRLHIFDSISLFIKSLGINEHYISLSRGVVDSR
                     DVIYLMSLTFGFLYLTKYNLDNKSK"
     gene            5164..6897
                     /gene="gldG"
                     /locus_tag="PKI65_06530"
     CDS             5164..6897
                     /gene="gldG"
                     /locus_tag="PKI65_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOB78131.1"
                     /translation="MKKQRNKITTDKKIKNKKWSPLIVFLVLITINILASFCYFRVDL
                     TSEKRYSLAPATKNVLKNLDDYVYFQVFLDGKLSPSYKRLQKTIIETLEEFRAHNKKL
                     IKYDLINPAKDKDESTLRNYLKELEYRGLVPSIDYEMTETGSSENIVWPCAIVTYKGR
                     ELPINFIYSNIPGSKEQLINDAIENTEYKLIDGIYRITLKEKSKIAFIQGHGELDALE
                     INDLAMSLLDYYIVNQIAINHQLKALDGFKAIIIAAPDSTFDELDKFIIDQFIMKGGR
                     VLWLIDGALADMDSLQDKPDILSIPNDINLDDMLFNYGVRVNKDLIMDYHSAPIPVKT
                     GQVGNQPQFSFFNWYFFPTVSNIKGHPIVRNINDIKFEFASSLDTIAKPGIKKTILLA
                     SSNYSRILNTPSIISLNFLRIKADRSFFNRSNIPMAVLLEGSFTSLYKDRIPDTIAKD
                     PEIKFSEYSQPNRMIVIGDGDVAKNQVIYRQGKYIPYPLGYDRYTGIMYGNREFLLNC
                     VNYLLDEADIISIRNRDISIRLLDKRKVEINQAVIKIINIALPLAIVCFCGILISYIR
                     KQNNKKKISKL"
     gene            complement(6995..7711)
                     /locus_tag="PKI65_06535"
     CDS             complement(6995..7711)
                     /locus_tag="PKI65_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78132.1"
                     /translation="MKYIINKKFLLLICLFIIIPNHLYQQNDKCKYGIRNYDFTKKHN
                     PRNQKRQLSNYLDYVNNDKTLKNIDKFGKIKTHRDDVNELIGVGNNDYFVLMPDSLVG
                     IFKIKKIIEKKNNYQKIENKLIKRSIYFIDLECVNIYNDTCPDFIRLLSIKPLDKISN
                     KKLRKIKIGEEYQMKIFSFFKADCCIMMNENGEIITIVRHPNSFRTSYLIYDIWVPYI
                     DIGSYNLFETKNLKGLYYIK"
     gene            complement(7788..8558)
                     /locus_tag="PKI65_06540"
     CDS             complement(7788..8558)
                     /locus_tag="PKI65_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78133.1"
                     /translation="MNKNFYKFLIILNISIFIRFNIFSQNIINICDFNNNVRTININD
                     YFYQTINYDSVIHEHKGFDSLTQKPDTIQISENLISIKKPSFIEVINDDPNFYYSILY
                     CTIQIFYDNKEIFFKDSVLTAGNYYFDEKYNILLIPLIIDQDIDDLFTTTILYYCNLN
                     NYDIKLIVNNLSNSSSAITACDGGKIIFNSSDKLYVFDLKTYKYYKIVEFDNTFIKIS
                     KIDIKDNYIVLYYINNYPDDLLNDPFMLNIAKIPINQN"
     gene            complement(8551..9279)
                     /locus_tag="PKI65_06545"
     CDS             complement(8551..9279)
                     /locus_tag="PKI65_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOB78134.1"
                     /translation="MFTAKELDNETSYTYFGARYYDADLSIWLSVDPMSDKYPSLSPY
                     CYSTWNPLVFKDIKGLDLVKVVVPANKDASKTKTIMVDSKIADKVHDFAYAMNAKYGL
                     VITSAFRSKEYQENLIKKWDSGDHSGLAVRPASTSAHSSGFALDFNVKEVFGLTSSNI
                     NTETNKELVKELTDFAAEYGFSYGGNYKKPDPPHFYINESDYGYENRNEAIEVNDQYL
                     KNHTEDNIPYYNPNKNENNNKKNE"
     gene            complement(9392..9808)
                     /locus_tag="PKI65_06550"
     CDS             complement(9392..9808)
                     /locus_tag="PKI65_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78135.1"
                     /translation="MKKINILLVLFVVLFFINKIPIYSQKYEIKKITLYIVPINSKEG
                     FDIPYNRIKAYSSLKITIKHWVESAILSKMLYGFKCETIAEINKDFRVLCIIHWGYKK
                     EKIYFNKYGHFLYNGIACKNDEIWEYILSFIPDKYE"
     gene            complement(9832..10266)
                     /locus_tag="PKI65_06555"
     CDS             complement(9832..10266)
                     /locus_tag="PKI65_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78136.1"
                     /translation="MKKFILPLLAILGLNFNFLTLHSQEVFTTDCCIRKITLYIIPPG
                     YLFRRQIDYYEVKKKSDIKLSIKSPSQLDDSNFLQLLQNLNENDKTEIIQDYRIKCII
                     HKFLGREVLYFNAFGGFLYKGQTYYNEDIKNLIWRYYLPTKF"
     gene            complement(10278..11108)
                     /locus_tag="PKI65_06560"
     CDS             complement(10278..11108)
                     /locus_tag="PKI65_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOB78137.1"
                     /translation="MIDAIVRHYISHTFCLENNYHYSNYKELDNETNYTYFGARYYDS
                     ELSVWLSVDPMSDKYPSLSPYCYTADNPVVLVDPNGNEIRIYYKEKKGISRLFERSKY
                     ITYTPGMKYTGENNFVSNAVDSLNYIYNKNADVEGIIQQSVATSEIVNVKKSFSDYYD
                     DSKNTIYWSTKGMKTFYKINGTYEVGKQSGALGLFHELGHFYRDIFKHDEFEKEKNMI
                     DQDYDFVEERRVIEEYETPAAIILNQGIRNNHWAKILNTVDPLSTKTRKEQKNNVPML
                     "
     gene            complement(11053..11703)
                     /locus_tag="PKI65_06565"
     CDS             complement(11053..11703)
                     /locus_tag="PKI65_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78138.1"
                     /translation="MIKFIFNLFLIFNFISHSCFTNFFENPIIKDTLQVKEIDEGYLI
                     YIDNVNIIKLVNDSINVTYLILHDKNKVKLDFYIIPPYLTGTYLLNLNEFGKVLALES
                     YPVGTSGLSANIINVSLIFLDKNFKVLSYNSFNGGINLLFINNNKFLLNIYDYNGIDD
                     NGNIKYCLNIFELTKDSFKRTDSLTKCYIYTEDKQLKFYDRCNCSTLYKPYVLSRE"
BASE COUNT         4227 a         1841 c         1444 g         4617 t
ORIGIN      
        1 tttatacagg gacttgagag ctaagccaat attaaattta acataaaaaa atttttcaat
       61 atttctaact tcatttttaa cgcttaatgg ccgtccaaaa ttaaagtatg ttaaatacat
      121 aaatttatat aagcaatgac attaactatt ctcatggcaa acgtctgagt aagtatatat
      181 taaaaccttt atattagtca aaataaatta taatgaacaa ataaatacag ttgcctacaa
      241 cttcttaacc tctcgctcgt ctttttctaa tttcatcttt tcattctctc ccttttcact
      301 ctactgtttc catgctctat acacccttaa taatatgatt taatgttata aatttatttg
      361 tgtaataaat actattatac tttaaaacta aatatttgta ttttaacatt tttaattatt
      421 tacaataatc tctgcagctt ttttgctagc accactatca cctaaaattt tttttaattc
      481 atcgaaatgg tctagcattt gctgtctata tgttaaatca aagagtattt tatctaattc
      541 tcttcgtata ttttcggcac tacatttctc ttggatcaat tcttttacga tttcctcacc
      601 ataaatcaaa tttactaaag aaataaaaga tattcgagcc attatccttg ctattagata
      661 agatatccaa tttgctttat aacaaacgac caaaggtaca tcgaaaagtg cagcttctaa
      721 tgtagcagtt ccacttgtaa caattgctac ataagaactt tgcaataaat tatacgtatc
      781 atttataagc aattgatcag ttgttactcc agagttataa taaatatttt tatcaataaa
      841 tgataaacca gccacataaa tttgaaattc tttttcatat ggtttaacag ctttcatcat
      901 tataggtaaa agaagtttta tctcttgaat cctactacct ggcagcaaag caataatagg
      961 tttattagag tggatattat ttaaactact acacttaaat gtatcaatat gatctagtaa
     1021 aggatgaccg acgtaatgta catctgtact aaatttttta taaaattttt tttcaaaagg
     1081 caaaatagat attggaacat ctatatattt ttttattttt ttagctctag atctattcca
     1141 tgcccatact tgtggtaata tataataata tattttaaaa ttatttttgc gaagccattt
     1201 agccactctt aggttgaatc caggaaaatc aattaaaatg atcttatcag gttttaattc
     1261 aattatagtt tctttaaatt ctttaaaatt ttttaatatc tttttcaaat taattaatac
     1321 ttctacaaag cccataaatg ccaattcagt aataggctta gtaatattta caccagcatt
     1381 tttcatcaat tcaccacccc atccatagat aatagcttct ttatcaatag caaaaattgc
     1441 tttagctaaa ttgcttccgt gcaaatcacc tgactgttct ccagataaaa taaaatattt
     1501 cataaaatca acttacaatt aatcattaat tatcatcatc taattaataa gattatgaca
     1561 attaaaaata gcacaatttt acaaaaaata taattactaa aacagaaaaa attaattttt
     1621 attcctattt ttattattat ttgtgttatc tttatttaat aaagctttat gagatagttt
     1681 aaatttacca gtttttgcat caatatcgag aattttcact tgtacgtgat cacctatttt
     1741 atagtaatca tttacatcat ttaccctttt attatctatt tctgatatat gtaataaacc
     1801 ttctttgcca ggtaaaattt caacaaaagc accatagctt tgtatagact taactatacc
     1861 atcatacaca tcgcctatct ggggcactgt agtgatagct ttgatttttt taatagcttt
     1921 ttctaagcct tctttattag tagagaatat atctacgata gccatatctc cagcttcttc
     1981 tatcactata gtactatcag tttccctttg tagttcttgt atgactttac caccaacacc
     2041 tatcacagca cccataaatt cgcgtggaat agttaattta gcatatctag gtacattagg
     2101 tttatagtca gatctactaa tagaaatggt ttgattcata atatctaaaa tatgtagcct
     2161 acctttttta gcttgttgta atgcttctga aagtatttga aatgataaac ctttgatttt
     2221 aatatccatt tgacatgcag taattccatc agttgtacca gcgactttga aatccatatc
     2281 accgagatgg tcttcatcgc ctagtatatc actcaatata gcataattac cgcttttttc
     2341 gtccattatc aaacccatag ctataccgga tacagattta tgcattttaa cacctgcatc
     2401 catcaaagct aatgaaccag cgcataccgt agccatagaa gagctaccat tagattctaa
     2461 aatatctgat actacacgaa tagtataagg attatcttca tcgcgtatta tcatattttt
     2521 caaagctcgt agagctaggt taccatgtcc tatctctcta cgtcctaaac gaataggttt
     2581 tacttcacca gtagagaatg gtggaaaatt ataatgtaat aaaaaattac ttgtaccctc
     2641 ataaactgca ccatctatta attgctcatc tagtttagtt cctaatgtta cggttgtcaa
     2701 actttgagtt tctcctctag taaatattgc tgacccatgt gcagatggca aataatcaac
     2761 ttcgcaccaa atagggcgaa tttcgtctaa acgacgtcca tcgagacggc aacgctcatc
     2821 taataccatc tgacgaactg ctcttttgtg aacctcatga aaatatctat taaccattgg
     2881 gtagatctct tctctctctt catctgaata ttgattcaaa aaatcttctt ttatggcttc
     2941 gaactgttgt tgacgttctt ttttagactt tatatgttga cgtgatattt ctaataagcg
     3001 aggatagcaa tattcttcta gtttttgacg aatttcttca ttatttacct cgtgctcata
     3061 ttttctttta ggtttacctg ctttttctat taatcttatc tgtccttcta tctctatttt
     3121 tatatattca tgagctattt ttaatgcttc cagtaatgta tcttcatcca cctctctgca
     3181 ttcaccttct accatattta catcagtgag tgtaccacct accatcaagt ctaaatcagc
     3241 cgattctatt tcagtaattg atggatttac tataaattcg ccatttattt taacaactct
     3301 aacttcagag ataggttgct caaaaggtat atccgatata gtgagtgctg acgatgcagc
     3361 tagagcagtt aatgcatcag gtgggtaatt tttgtcagca cttattagat aaattaagac
     3421 ttgtgtctcg gcgtgataat cttcaggaaa aatgggtctc aatgctctat ctactaatct
     3481 agcaattaat atttcatatt ccgatggtct agattctctt ttcaaaaaac ctcccggaaa
     3541 acgacctgca gcagcaaatt tttcttgata ttctactgtc aatggcataa aatcaacttc
     3601 tggactagct tcatcctttg atactgctgt agctaaaagc atagtatccc cgcattttaa
     3661 aactacagca ccatctgctt gtctagctaa tttacctgta gatatttcta taatctgacc
     3721 attaggtaga ttaattattt cttttatttc ttgcatttgt taagtgtttt tattttttgt
     3781 ttaaaattta tttttttcta aaaggtaaaa ttataaaaaa atattggaac gattataata
     3841 taaattcaaa attttaactg ataatataca ataaataact acaaaaaaat tttcaaatga
     3901 tttcttaggt atttaagcat caaatgaacc aaaaataata tttttaaaaa tcagaaaaaa
     3961 ttaaaatttt tatacttttt gtaattacgt attattattt tcactgaagt aaacaatgtg
     4021 atattatttc cttatattta attgtttagt tacttctaga tatctatcgt aatcttttct
     4081 tttcaaatat ttaagcaaac gcttacgttt attaaccatt ttaagaagtg aacgcttagc
     4141 tgaaaaatct tttttatttt ctttcagata ttcagtcaaa tataatattc tctcagtaaa
     4201 aatagcaatt tgaaccgctg tatcgcctgt attattttca cttccgccaa attttattgc
     4261 aagatctctc gtcttttctg gtgtcaaata cataatttat aattttagtt tgcaaaatta
     4321 caaaaaaaat taattacaaa aaaataaatt tagttttttg taatttatta tctttgcaaa
     4381 tcaaataaca aaaaacatgt gggcattata taaaaaggaa ttatctgatt tttttacatc
     4441 tttagtaggc tacatcgtca tatgtatttt tttacttgct actggcttgg tattgtggtt
     4501 gtttccaaat gaatttaaca tcactttagg cggatatgca gtattagatg gcttatttat
     4561 attagcgccc tttgtatttt tatttctttt accagctatt acgatgcgta tgtttgctga
     4621 ggaatataat actggtacta tagaattgct tttcaccttt cccatatcac gttggcaaat
     4681 aatttttagc aaatttttag ctggttttac tatttttctt ttaagtctac ttccgacatt
     4741 aatatatttt tatagtgttt atcagcttgc tttacctgta ggtaatgtag atataggcgg
     4801 agtagcaggt tcatatattg ggctaatact attaggaatg gcttttttag ccttaggcat
     4861 ttacagctct tcgatagcca ataatcaatt aattgccttt ctcatagcat ttgtactgtc
     4921 acttttcttt tatataggct tcgaatatat cagtcgttta catatatttg attctattag
     4981 cttatttata aaaagcctag gaataaatga acattatatt tccctcagta gaggcgtggt
     5041 agatagcaga gatgttattt atttgatgag cttgacattt ggttttttat atttaactaa
     5101 atataattta gataataaaa gcaaataaat actatatata aaatacaaaa taatgaagta
     5161 aaaatgaaaa agcaaaggaa caaaataaca actgataaaa aaataaaaaa taaaaaatgg
     5221 tccccattaa ttgtattttt agttttgatt acaatcaata tactagctag tttttgctac
     5281 ttcagagtag accttaccag tgaaaaacgt tattcactcg ctccagctac taaaaatgta
     5341 ttaaaaaatc ttgatgatta tgtatatttt caagtttttt tagatggcaa actctcacct
     5401 tcatataaaa gattacaaaa aactatcata gaaactctcg aagaattcag agctcataat
     5461 aaaaaattga taaaatacga tttgataaat cccgctaaag ataaagatga aagcacactt
     5521 agaaattatc taaaagagtt agaatataga ggtctggtac catcgataga ttatgaaatg
     5581 actgagactg gttctagtga aaatattgta tggccctgtg ctatcgttac atataaaggt
     5641 agagagctgc caataaattt tatttattcc aatattcccg gttctaaaga acaactcata
     5701 aatgatgcca tagaaaacac agaatacaaa ttaatagatg gtatttatcg cattacatta
     5761 aaagaaaagt ctaaaatagc atttatccaa ggacatggag aactagatgc tttagaaatt
     5821 aatgatttag caatgtcatt attagattat tatattgtta atcaaattgc tattaatcat
     5881 caattaaaag cattagatgg cttcaaggct ataataatag cagctcctga tagcaccttc
     5941 gatgaattgg ataaatttat tatcgatcaa ttcattatga aaggtggtag agttttgtgg
     6001 ttaatagatg gtgcattagc tgatatggat agtctccaag ataaaccaga tatattgtct
     6061 atacctaatg atataaatct agacgacatg ctctttaatt atggagtgag agtaaataaa
     6121 gatttgataa tggactatca cagtgctcca atacctgtaa aaactggaca agttggaaat
     6181 caaccacaat tttcattttt taattggtat ttttttccta cagtttctaa tatcaaagga
     6241 catcctatag tcagaaatat taatgatatt aaatttgaat ttgccagtag tttagataca
     6301 atagcaaaac ctggaattaa aaaaactata ttgctcgcat ctagtaatta ttcacgaatt
     6361 ttaaatacgc cctctatcat atcattgaat tttttgagaa ttaaagctga tagatcattt
     6421 tttaatagaa gcaatatacc aatggctgtt ttactagaag ggagttttac tagtttatac
     6481 aaagatcgaa taccagatac tatagccaag gatcctgaaa tcaaattttc tgaatacagc
     6541 cagcctaata gaatgatagt gataggtgat ggtgatgtag ccaaaaatca agttatttac
     6601 cgccaaggca aatacatacc atatccttta ggttacgaca gatataccgg cataatgtat
     6661 ggaaatcgtg aatttttatt aaactgcgtc aattatttat tagacgaggc agatattata
     6721 tccattagaa atcgagatat tagtatcaga ttattagata aaagaaaagt agaaattaat
     6781 caagcagtta taaagattat taatatcgca ttgcctttag ctatagtctg cttttgtggc
     6841 atattaattt catatataag aaaacaaaat aataagaaaa aaattagtaa attatgagct
     6901 ccaaagcaat tcataaatta ttcttttgtt acttttcttt gcccgccaca caaagaaaac
     6961 taacaaaaga aagatactgg atgtgctgaa aaaatcattt tatataatat agtcctttta
     7021 aatttttagt ttcgaataaa ttataactac ctatatcaat gtatggcacc caaatatcat
     7081 aaattaaata tgaagtacga aaactattag gatgtctaac aattgttata atttcaccat
     7141 tttcattcat cattatacaa cagtctgctt taaagaatga aaatattttc atttgatatt
     7201 cctctcctat ttttattttt cttaactttt tatttgatat tttatctaat ggttttattg
     7261 ataataatct aataaaatct ggacatgtat cattataaat atttacacat tctaaatcta
     7321 taaagtaaat tgatcttttt ataagtttat tctcaatttt ttgataatta tttttctttt
     7381 ctattatttt tttaatttta aaaataccga caagactatc tggcattaaa acaaaataat
     7441 cattattacc gacacctatt aattcattaa catcatctct atgtgttttt attttaccaa
     7501 atttatctat attttttaat gtcttatcat tatttacata atctaaataa tttgataatt
     7561 gtcttttttg atttcttgga ttatgttttt ttgtaaaatc ataattacga ataccatatt
     7621 tacacttatc attttgttga tataaatgat taggtataat tataaacaaa catattaata
     7681 agagaaattt tttattaatt atatatttca tggcacccta ttatatttat atattttaaa
     7741 ttcatccata aaaccttgac ctccaatata aatttcttca atatttgtta attttgatta
     7801 attggaattt ttgcaatatt taacataaaa ggatcattca ataaatcatc tggataatta
     7861 ttaatataat ataacacaat ataattatct ttaatatcaa ttttagatat tttaataaat
     7921 gtattgtcaa attctacaat tttgtaatat ttatatgttt ttaaatcaaa aacatatagt
     7981 ttatctgaac tattgaaaat gattttccct ccatcacatg ctgtgattgc agaacttgaa
     8041 tttgataaat tatttacaat aagtttaata tcataattat ttaaattaca ataatataaa
     8101 attgtagttg taaataaatc atctatatct tgatcaatta ttaagggaat taacaatata
     8161 ttatattttt catcaaagta ataattgcca gcagtcaata cactatcttt aaaaaagatt
     8221 tctttattat cataaaatat ctgaattgtg caatataata tagaataata aaaattggga
     8281 tcatcattta taacttcaat aaaacttggt ttcttaatgc ttattagatt ttctgatatt
     8341 tgaatagtat caggtttttg agttaaacta tcaaaaccct tatgctcatg tataacgcta
     8401 tcataattaa ttgtttgata aaaataatca ttaatattaa ttgttcttac attattatta
     8461 aaatcgcaaa tatttataat attttgagaa aaaatattaa atcgtataaa tatactaata
     8521 ttaagtataa ttagaaattt ataaaaattt ttattcattt tttttattat tattttcatt
     8581 tttgtttgga ttataataag gaatattatc ttctgtatga tttttaagat attgatcatt
     8641 tacttctata gcttcattcc tattttcata tccataatca gattcattaa tgtaaaagtg
     8701 tggtggatca ggttttttat aatttccacc ataagaaaaa ccatattctg cagcaaaatc
     8761 tgttagttct tttactaatt ctttattagt ttctgtatta atatttgacg atgttaatcc
     8821 aaatacttct ttcacattaa aatctaatgc aaatccagaa gaatgagcac ttgttgatgc
     8881 tggtctaact gctaaacctg aatgatcacc actatcccat tttttaatta aattttcttg
     8941 atactctttt gaacgaaaag cactagttat taccaaacca tattttgcat tcatagcata
     9001 agcaaaatca tgaaccttat ctgctatttt tgaatctacc ataattgttt ttgttttact
     9061 agcatcttta ttagcaggaa caacaacctt aaccaagtcc aaccctttaa tatctttaaa
     9121 tactaaagga ttccaagtac tatagcaata tggcgatagc gatggatact tatcgctcat
     9181 cggatccacg ctcagccata ttgacaagtc cgcatcatag tatctcgctc cgaagtacgt
     9241 gtaactggtc tcattgtcta gctccttggc ggtgaacata aaaatatttt ttgttaggta
     9301 aaattaataa atattttttt gaataattta ttttaaatga taagatattt catttttaaa
     9361 agaaataggt acattataaa acaaaaaact attattcata tttatctggg ataaatgaaa
     9421 gtatatattc ccaaatttca tcatttttac atgctatacc attatataaa aaatgaccat
     9481 atttattaaa ataaattttt tcttttttat atccccaatg aatgatgcat aatactctaa
     9541 aatctttgtt tatttctgca attgtttcgc acttgaaccc atataacatt ttagataata
     9601 ttgctgattc aacccagtgt tttatagtaa tctttaatga tgaatatgct ttaattctat
     9661 tatatggaat atcaaaacct tccttactat taattggaac tatataaagt gttatttttt
     9721 taatttcata tttttgtgaa tatattggaa ttttgtttat aaaaaacaac actacaaata
     9781 atactagtag aatattaatt tttttcataa ttataattat taaactttat attaaaattt
     9841 cgtaggtaaa taatatctcc atataagatt ttttatatct tcattataat aagtttggcc
     9901 tttatacaaa aaaccaccaa aagcattaaa atataaaact tctctaccaa gaaatttgtg
     9961 aataatacat tttatcctat aatcttgaat aatttcagtt ttatcatttt catttagatt
    10021 ttgaagaagt tgtaaaaaat tggagtcgtc cagttggctt ggagatttaa tagaaagttt
    10081 aatatcagat ttttttttaa cttcatagta atcaatttgc cttcggaaca aataacctgg
    10141 aggaataata tataaagtta tttttcttat acaacaatct gtagtaaata cttcttgtga
    10201 atgtaaggtt aaaaaattaa aatttaatcc taatatggct aataatggta aaataaactt
    10261 tttcatattt tataatttta taacattggc acattatttt tttgttcttt tctggtctta
    10321 gttgatagag gatctacagt atttaaaatt ttagcccaat gattatttct aattccttga
    10381 tttaatatta ttgctgcagg tgtttcatac tcttctatta ctcttctttc ttcaacaaaa
    10441 tcataatctt gatcaatcat atttttctct ttttcaaatt catcatgttt aaaaatgtct
    10501 ctgtaaaaat gacctaattc atgaaataaa cctaaagctc cagattgttt acccacttca
    10561 taagtaccat tgattttata aaatgttttc attccctttg ttgaccaata aattgtattt
    10621 tttgaatcat cataataatc actaaaactc tttttaacat ttacaatttc agatgtagca
    10681 actgattgtt ggattattcc ctctacatca gcatttttat tataaatgta atttagtgaa
    10741 tcaacagcat tactaacaaa attattttca cctgtatatt tcataccagg agtataagtt
    10801 atatacttag atctttcaaa caatctacta ataccttttt tttctttata ataaattcta
    10861 atttcattcc cattcggatc taccagcacc actggattat cagctgtata gcaatatggc
    10921 gatagcgatg gatacttatc gctcatcgga tctactgaca gccacacact cagctcgcta
    10981 tcatagtatc tcgcaccgaa gtaggtgtag ttggtctcat tgtctagctc tttataattt
    11041 gagtaatgat aattattctc tagacaaaac gtatggctta tataatgtcg aacaattgca
    11101 tctatcataa aattttaatt gtttatcttc tgtgtaaata taacatttgg ttaatgaatc
    11161 agttcgttta aaactatctt ttgttaattc aaaaatattt aagcaatatt ttatattacc
    11221 attatcatct atcccattat aatcataaat attcaacaaa aatttattat tatttataaa
    11281 taatagatta ataccaccat taaatgaatt gtatgataaa accttgaagt ttttatctaa
    11341 aaaaattaga cttacattta ttatattagc acttaaacca ctagtaccca ctggataact
    11401 ctcaagagct aaaacttttc caaattcatt taaattcaat aaatatgtac ctgttagata
    11461 tggaggaata atataaaaat ctaatttaac tttatttttg tcatgtaaaa taagatatgt
    11521 tacattaatg gaatcattaa caagttttat aatattaaca ttatctatat atattaagta
    11581 accttcatct atttctttta cttgtaatgt atcttttatt ataggatttt caaaaaaatt
    11641 tgtaaagcat gaatgactaa taaaattaaa aattaaaaat aaattaaaaa taaattttat
    11701 catggccatg tttttttgga taaagatatt gattatattc cataaactgg gtacctccaa
    11761 tctttttagt ataagaaaat gtaattttgt taggtaaaat taatgaatat ttttgaataa
    11821 tatctactta atttactttt ctttgtcagc cacacaaaga aaagtaaata aaagaaaagt
    11881 ctccgctggt tttaacaccc ctgtctctgt gagacatcca ctctacaaga ggggaatttt
    11941 ttcgagcaga attccccttc tccgaagggg tgtcaggctt tgcctgacgg gatagttaaa
    12001 aaaactacgc tgccagccct acccaaccct gcttgcttgt tttattacaa atcatttccc
    12061 aatgcgggcg ggacaaaata aaaacacaca gcacacaatt aaatataatt gattatgaaa
    12121 ttattctta
//
LOCUS       DAOPRN010000056        12054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_4469_length_12054_cov_148.523044, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000056 DAOPRN010000000
VERSION     DAOPRN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12054
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4469_length_12054_cov_148.523044"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PKI65_06570"
     CDS             <1..717
                     /locus_tag="PKI65_06570"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78139.1"
                     /translation="KEKMLDYIRCMLEGKTLRACAKEVGISLPTSFAWRHRILAALRN
                     FEANVNFFGIVEVEELLMNYSEKGRRYRNEEELAEAREKKKKKVAVIASKDRTGNMLF
                     KQLGLGKIKREQIEQLLNTKVSANSVICFGHNDEFRKLKSQNLERKEIVIDRVRKRGV
                     YSVATIRRHVNTFVGWINNQFRGVATKYLQSYIMWYVVMHKYLLNNVVPDVGRMLNLA
                     SSDRRAWYVYRDLRASPVFI"
     gene            complement(1047..3509)
                     /locus_tag="PKI65_06575"
     CDS             complement(1047..3509)
                     /locus_tag="PKI65_06575"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HOB78140.1"
                     /translation="MRKKIVIVGGVAGGSSAAARLRRLDEFAEIIIFERGEYISFANC
                     GLPYYIGGIITNKNALIIQTPEIMSKRFNLDIRTNNEIIKIDPKSKYVTVKNLKTNEI
                     YNESYDKLILSPGAQPIKPSLPGIKKSDNLFTLRTIPDADAIISYIIEKKPKNAVVIG
                     GGFIGIEIAENLHARGLHVSIVELTEQILTQIDLEMASILHNHIKSKGIELILGDGVK
                     EFKEQGAKIVLNSNRELSTDLTILAIGVKPEINLAESAGLEIGVTGGIKVNEYLQTSD
                     ENIYAIGDAIEVRDYITGHPTLIPLAGPANKQGRIVANNICGKKEKYLGTLGTSVVKV
                     FDMTAAFTGNNEKMLQAKGITYKTIYISPSSHATYYPGATSIHMKLIFDSETGKILGA
                     QAVGYEGVDKRIDVIASVMRLNGTVYDLTELELAYAPPYSSAKDPVNMIGFVAENQLE
                     GLVDVVLCRELEELDRENTVILGVFSEEEFQVGSIEGSINIPLEELRDRLDELDKEKL
                     YITYCAVGLRGYIAARILMQHGFKVKNLAGGFNMYKKFHFKHDEIISNSDDIIEFNDS
                     GIQKIQNKATGEVYKLNACGLSCPGPIIKVSEKLKTLNEGDRLEVISTDPGFMNDIKS
                     WCKNTGNTLISAEKIDDKYVAIIEKGNKSKKESTEEMAPIKDGKNLIIFSGDLDKAIA
                     SFIIANGARAMGKEVTMFFTFWGLNIIKKETQVKTKKDFMAKMFSKMMPKNSKKLGLS
                     KMNMAGIGPKLIRKVMKDKNIQSLEELIDEAMKSGVRMVACQMSMDVMGVAAEELLDG
                     VEIGGVATMLAASDESNMSLFI"
     gene            3757..3830
                     /locus_tag="PKI65_06580"
     tRNA            3757..3830
                     /locus_tag="PKI65_06580"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3791..3793,aa:Ala,seq:ggc)
     gene            complement(4100..6379)
                     /locus_tag="PKI65_06585"
     CDS             complement(4100..6379)
                     /locus_tag="PKI65_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOB78141.1"
                     /translation="MTDLYTKSLNKEQLEAVRYLDGPMMIIAGAGSGKTRVLTYKILY
                     LLEHGYSPENIMALTFTNKAANELKGRIYQYVGVNKARAISAGTFHSVFSKILRKNAE
                     YIGHNSNYTVFDSDNTKSLLKEIIKYLKVSEKHKVDIVQRRISQAKMQLIDPFQYADS
                     QDLIIDDTKFGLPHIVDIYLEYWQRCRKMNVLDFDDLLLFTHKLFDKFPEILRNYQDQ
                     YKYILVDEYQDTNYAQYVIVKQLADLNEQLCVVGDDSQSIYSFRGANIGNIFQLKKDF
                     PNLKTFVLTKNYRSTQRIVQVSNNLISHNKAKIDKKIVTENELGEPIILFQCSDEHDE
                     ARKVVGAIFQLIQTYHYDYKDFAILYRVNALSRALEDELRRLNLPYRIYGGMSFYERK
                     EIKDAIAYFRFISNPNDVESFKRIINVPPRGIGDITAMKLQEAFYLNDVPFTTLLAHI
                     DEYNLDINKPTQLRIKNFHALMEKYRQLASKLDAFDMAKKVIYDTGYYKYAELDSHER
                     AENLDALLSGIQDYTEHFIDENEGLSPTIEDYLSVISLITSNDEIQDDNVISLMTIHN
                     AKGLEFDNVFVIGVEENLLPHYNSLMDLRDIEEERRTFYVAITRAKKRLYLSFAFSRF
                     SHGVTRFNEPSRFIFEMEKEHFDTKSKNLFLKNPYQRQSITANNKSDNYNKKALGPSS
                     KTKIGKTSSDVETANYYLGINLLNKLKEGMIIEHNKFGRGEILNIEGNSTEAKATVNF
                     DKSGKKHIILKFAKIRILD"
     gene            6567..8240
                     /locus_tag="PKI65_06590"
     CDS             6567..8240
                     /locus_tag="PKI65_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOB78142.1"
                     /translation="MIAFLYPICVYSQIDDNIDINIDSSDIAFAFDRAVIELYYNNYP
                     SNFERISIEQNYYRSFTDDELEALLNDSLIARRIAIMNVNSPFEYRYNKDVKQWIKYY
                     LRHRQFLGRIIGLSELYFPIFEETFSKYDIPLELKYLAVVESALNPKAKSRAGAMGLW
                     QFMYRTGLLYDLNVTSYVDDRCDPYKSTEAAARHMRDLYDIYNDWALVLAAYNAGAGR
                     INRAVLNSGGKTNYWEILPYLPKETRNYVPAFIAISYIMTYYKELNITPSPPKFKDVE
                     IDTVTIRYSTSLKAISDFFNISYEDLKFLNPQFIKDVVPSTEHQMYCIRIPRKYRMLF
                     YSKEQEFYDSLLVKRDKVDTSYSSSVKSETPPASNVIYHKVQKGQTLSSIASKYRVTV
                     SDIKKWNNLKSNMIYVGQKLKIYTNTPAKSPTSTSSSTTASTSSEKSSQSQTSSSSNV
                     TTKTQNITHIVKSGETLSGIANKYGVTITDIKKWNNLTSDKIVVGQKLIINPTQKSIS
                     SSGNKVHMVKSGETLSSIALKYNTTVNEIMKKNNLKSTKIVVGQKLIIP"
     gene            8237..9214
                     /locus_tag="PKI65_06595"
     CDS             8237..9214
                     /locus_tag="PKI65_06595"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOB78143.1"
                     /translation="MKKYFFAILLLLLVSLSCKEQANMPVSIGKINSVIVVLRNGVSQ
                     TPASKTLDSILTQEIYGMPQSESLFDVAHVTEANLTDLLKKNRNIIYLIISDTVKKDG
                     IFMSEDVFATPQTILFIRSSHPDSAKKLIEKSENLIINTLKNTEYRRIYEIMSKSPAV
                     DIKKKINENFGIDLQLINLFFEAKSTKDFMWLRLETNNYSQGLILMKEPLISENQFNP
                     SHIRPWIDSTLKLHIPGQFEGTYMKIDDSFIKLKIDRVVINDIERIVFTGLWGVQGDF
                     MGGPFRSHTFLSKDKTHLITVYAYVYYPSEEKRNLMMDLEAILYSIKFV"
     gene            complement(9325..9972)
                     /locus_tag="PKI65_06600"
     CDS             complement(9325..9972)
                     /locus_tag="PKI65_06600"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOB78144.1"
                     /translation="METGRIPLIGEPLPQMKVLTTHGPKVIPDDYKGKWLILFSHPGD
                     FTPVCTTEFYAFSKKYDEFKKLNAELLGLSVDQVFAHIKWVEWIEENLKQEVPFPIIA
                     DPLGSVSNALGMIHPNKGSETTRAVFIIDPNSIIRIILYYPQEIGRNVDEIIRSLRAL
                     QMNDKYKVSLPHNWPNNELIGDKGIIPPPKNIAEIPKRKKEYEGYDWWFTYKDLK"
     gene            complement(9948..10391)
                     /locus_tag="PKI65_06605"
     CDS             complement(9948..10391)
                     /locus_tag="PKI65_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOB78145.1"
                     /translation="MDPLKVLKKHNLKATIQRIKILEYLMNTTSHPSVEEIYSTLKLI
                     MPTISLSSIYNILSEFEKNNIVTKLMTSDNIIRYDYKKDKHHHIYYEDGKIEDLEDAE
                     LDNILNEYFKTKYPHLVNDNEINLIINIKTITPKNKRLWKQEESP"
     gene            complement(10873..11973)
                     /locus_tag="PKI65_06610"
     CDS             complement(10873..11973)
                     /locus_tag="PKI65_06610"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB78146.1"
                     /translation="MKGLKTILSLALMLSVGLLMGQKQVSNETKNEISVKKTLDASMP
                     VKNTNETTTDTIGWTPGSIPEFGYPNSGLHIYGLTSGGYVGGVNGSDIDYWAQCYVLE
                     ESVSAIGILAWIGIKDNISEDNNSVLTASLLPTVEDEAITGLDDQGNPTSYGPGPDYY
                     SGNILASSSVNINDLDTTSYADPSLWQYFPFTNPVYISSDFCIVADFKSLRLNSDTAA
                     LVWDEFGNGLGLGYSQYCKDPTQYYWVACNCGLTFSGGQGLDANMSLFLIVEKGSDVN
                     SNSYFQGMQLTFPNLTSDPELIVQFALQNASNTSIDIISVNGQLVRSIDLGMKEAGSH
                     HTTIDISNLQSGMYFVSLNSQGSRLIKKLVIE"
BASE COUNT         4075 a         1880 c         1666 g         4433 t
ORIGIN      
        1 aaggaaaaga tgctcgatta cattcgttgt atgctcgagg gcaaaactct cagagcttgt
       61 gctaaagaag tgggcattag cctaccgaca agcttcgctt ggcgtcatag gattttagca
      121 gctttgagaa atttcgaagc caatgtcaat ttcttcggaa tcgtggaagt agaggaactg
      181 ttaatgaatt actccgaaaa aggaagacgc tatcgaaatg aagaagagct agcagaagca
      241 cgagaaaaga aaaagaaaaa agtagccgtt atagcatcta aagataggac tggcaatatg
      301 ctttttaagc aattaggctt aggtaaaata aaacgcgagc aaatagagca attgctaaat
      361 accaaagtat cagctaatag tgtgatatgt ttcggacaca atgatgagtt tagaaagttg
      421 aagagtcaga atcttgagcg aaaagaaata gtgatagata gggtaaggaa acgaggagta
      481 tacagtgtag caaccattag acgacatgta aatacttttg ttggctggat aaataatcaa
      541 ttcagaggtg tggctactaa gtacttgcag agctacatta tgtggtatgt agtaatgcat
      601 aaatatttgt taaacaatgt ggtgcccgat gtaggtagga tgctcaatct cgcatcatca
      661 gacaggcgag cgtggtatgt atatagggac ttgagagcaa gtcctgtgtt tatttaaaca
      721 cttaaatgag atctatcatt cccttatcaa cctctttacc ttcgtatttt ttatttcaac
      781 ttattatcta tataaaaaac aataaaacat cacatgaatc aaataaatac ttaagattta
      841 tagtacgtgt ttatttttta tagtcttgaa attacaaaat tcatgtaggg aaaatttcca
      901 aaatatttac aatataaagc actttatttt taaagaattt tactatttct taaaacttta
      961 acctttaagc attttatcat agttttgctt taatttttca aacaaattga gacaaaaggt
     1021 ctgacccttc gtcccaattt gtcccattat ataaataaac tcatattaga ttcatctgat
     1081 gcagctaaca ttgttgctac tccaccaatt tctactccat ctaatagttc ttctgctgct
     1141 actcccatta catccattga catttgacat gctaccattc taacaccaga cttcatagct
     1201 tcatctatta attcttctaa agattgaatg tttttatctt tcattacttt tctaattaat
     1261 tttggtccaa ttcctgccat attcatttta gataagccta attttttact atttttaggc
     1321 atcattttgc taaacatttt agccataaaa tctttcttag ttttaacttg ggtttccttt
     1381 ttaattatat ttaatcccca aaaggtaaaa aacatagtta cttctttacc catagctcta
     1441 gctccattgg caattataaa ggaagcaatt gctttatcta agtcaccgct aaatattatt
     1501 aaatttttgc catcttttat tggagccatc tcttctgttg attctttctt agatttatta
     1561 cctttttcaa taatggctac atacttatca tctatttttt ctgcacttat caatgtattc
     1621 ccagtatttt tacaccagga ttttatatca ttcataaatc caggatctgt agatataact
     1681 tcaagcctat ctccttcatt taatgtcttc aatttttcac taactttaat tataggtcca
     1741 ggacaggaaa gtccacatgc atttagttta tatacttcac cagttgcttt attttgtatt
     1801 ttttgaatcc cagaatcgtt aaattctata atatcatctg aattagatat aatttcatca
     1861 tgcttaaaat gaaacttctt atacatattg aatcctcctg ctaaattttt aactttgaat
     1921 ccatgttgca tgagaattct tgcagcaata tatcccctta gtccaacagc acaatatgtt
     1981 atatatagtt tttctttatc taattcatca agtctatccc ttagttcttc taatggtata
     2041 tttatacttc cttcaataga tccaacttga aattcttctt cagagaatac ccctaatata
     2101 actgtatttt ctctgtccaa ttcctctagc tctctacaaa gcaccacatc taccaaacct
     2161 tctaattgat tttcagctac aaaacctatc atatttactg gatcctttgc tgaagaataa
     2221 ggtggggcat atgctaattc taattctgtt aaatcatata ctgttccatt aagcctcata
     2281 actgatgcaa ttacatcaat tcttttgtct accccttcat atcctactgc ttgagcacct
     2341 aatattttac cagtttcgct atcaaaaatc aatttcatat gtatagatgt agcacctgga
     2401 taatacgttg catgtgaaga cggagaaata taaattgttt tatatgttat acctttagct
     2461 tggagcattt tttcattatt acctgtaaaa gcggcagtca tatcaaaaac tttaacgact
     2521 gaggtgccaa gtgtacctaa atatttttct tttttaccac aaatattatt agccactatt
     2581 ctaccttgct tattagctgg tcctgctaat ggtatcaaag taggatgacc cgtaatataa
     2641 tctctcactt ctattgcgtc gcctatagca taaatgtttt catcggatgt ttgtaagtac
     2701 tcattaactt ttattccacc agtaactccg atttctaatc ctgcactttc agccaagttg
     2761 atttcgggtt ttacacctat agctaaaata gtaagatctg tagataattc tctattacta
     2821 ttaagtacaa tcttagctcc ttgttctttg aattctttta ccccgtctcc taatattaat
     2881 tctatacctt tggatttgat atgattgtgt aagattgagg ccatttctag atctatttgt
     2941 gttaatatct gttctgtcaa ttcgactatt gagacgtgca aacctctcgc atgtaaattt
     3001 tcagctattt ctatacctat aaatccacct ccaattacca cagcattttt aggttttttt
     3061 tcaattatat atgatataat agcatctgca tcaggaatag tccgaagagt aaataaatta
     3121 tcagattttt ttattcctgg caaagatggt ttgatgggtt gtgcaccagg ggaaagtatt
     3181 aatttgtcat aagattcatt gtatatttcg ttagttttta aattttttac agttacatat
     3241 ttacttttag gatctatttt tataatttca ttattagtac gtatatctaa attaaatctt
     3301 ttagacataa tttctggagt ttgtataata agtgcatttt tatttgtgat aattccacct
     3361 atgtaataag gtaatccaca gttagcaaaa gaaatatatt ctccacgttc gaatattata
     3421 atctctgcaa attcatctaa tcttctcaat ctcgcagctg ctgatgatcc tccagctacg
     3481 ccgcctacta ttactatttt ttttctcata attattaatt tttttacaaa attaatagat
     3541 attaaatcaa ataactaaaa acatgacaaa tattaatcac actatgtgta tacttttgta
     3601 tattaaattt gctagtactt ttttaaacaa aaaaaaatta tttataaggg ttactctgta
     3661 aaattttgta ttttaacaaa atattttgaa gtttattgta aatttagatt ttaaaaaaaa
     3721 tttataattt tgcactttat aaagtaatat tacgtagggg cattagctca gctggctaga
     3781 gcgcttgcat ggcatgcaag aggtcaccgg ttcaaatccg gtatgctcca ctatttttac
     3841 ttaaatatca aaaatatatt atacaagatg gcgaaaaaat aaaaattcaa aacacaaaaa
     3901 tacgctatct ttcgtaatgt atatatacta tatatacccc aaagtattta ttttttttta
     3961 aattcagaga aaaacacgtg actttttttt aaaaaaaatt tttatacttc tcatttgctt
     4021 ccacttcttt ttgttacatt ctaccattta ttatcctcgt cacgtattta gcttttatta
     4081 tagtaatatt ttaaatactt tagtctaaaa ttctaatttt ggcaaatttt agaataatat
     4141 gttttttgcc acttttatcg aaattgacag tagctttagc ttctgtacta ttgccttcta
     4201 tatttaatat ttctcctcta ccaaatttat tatgttctat tatcatacct tctttcaatt
     4261 tatttaataa attgataccc aagtaataat tggcagtctc gacatcagaa ctagttttcc
     4321 caatttttgt tttagatgat gggcctaggg cttttttatt ataattatca gatttattat
     4381 tagcagttat actttgacgc tgatatggat tttttaaaaa taaattttta gattttgtat
     4441 cgaaatgttc tttttccatt tcaaaaataa atctactagg ttcattaaac cttgttactc
     4501 catgactaaa tcgagaaaaa gcaaaactca aatataatct tttttttgct cttgtgatag
     4561 ctacataaaa agtacgtctc tcttcttcta tatctcgtag atccattaga ctattataat
     4621 gtggtaaaag attttcttct actcctatta caaaaacgtt gtcaaattct aatcctttag
     4681 cattgtgaat agtcatcagt gatattacat tatcgtcttg tatttcgtca ttagaagtga
     4741 taagcgaaat aacagataaa taatcttcga tggtaggact tagaccctca ttttcatcaa
     4801 taaaatgttc ggtatagtct tgaatcccag ataataaagc atctagattt tcggctctct
     4861 cgtggctatc taactcagca tatttataat aacctgtatc ataaatcact tttttagcca
     4921 tatcaaaagc atctaattta ctagcaagtt gacgatactt ttccatcaaa gcatgaaaat
     4981 tttttattct taattgtgta ggcttattaa tatctaagtt atattcatca atgtgagcga
     5041 gtaaagtagt aaaaggcaca tcatttaaat aaaaggcttc ttgtaatttc atagcagtta
     5101 tatctccaat gccacgaggt ggtacattta taatgcgttt aaaagactct acatcattag
     5161 gatttgaaat aaagcgaaaa tatgctatag catctttgat ctcttttctt tcataaaaag
     5221 acatgccgcc atagatacga taaggaaggt ttagtcgtcg tagctcatct tctaaggctc
     5281 tagatagtgc atttacacga tataatattg caaagtcttt ataatcataa tgataagttt
     5341 gtattagttg aaaaatagca cctactactt tgcgtgcttc atcatgttca tcactacatt
     5401 gaaataaaat gattggttcg cctaactcat tttcagtgac tattttttta tcaattttag
     5461 ctttattatg acttattagg ttattagata cttgtacgat gcgttgagta gaacgataat
     5521 tttttgttag tacaaaagtt ttgagattag gaaaatcttt ttttaattga aatatattgc
     5581 caatattagc accacgaaat gaatatatac tttgtgaatc gtctcccact acacataact
     5641 gttcattcaa atctgctaat tgctttacta ttacatattg agcatagttg gtatcttgat
     5701 attcgtctac taatatgtat ttgtattgat cttgataatt tcttaatatt tctggaaatt
     5761 tatcaaataa tttatgtgta aaaagtaaaa gatcgtcaaa atctaataca ttcatctttc
     5821 tgcatctttg ccaatattct aaataaatat ctactatgtg aggcaatcca aatttagtat
     5881 catctatgat cagatcttga gaatctgcgt attgaaaagg atctattagt tgcattttgg
     5941 cttgagaaat tcgacgttgc actatatcta ctttatgctt ttcgcttacc tttagatatt
     6001 ttattatttc ttttaataaa cttttagtat tatcggagtc gaatacagta taattgctat
     6061 tatgtcctat atattctgca tttttgcgaa gaattttaga aaaaacacta tgaaaagtac
     6121 ctgcagagat agctcttgct ttatttacac ctacatattg ataaattcta ccttttaact
     6181 cgtttgcagc tttattagta aatgtaagtg ccataatatt ttcggggcta tacccatgct
     6241 ctaatagata taatatttta tatgtgagta cacgcgtttt gccactacca gcaccagcta
     6301 ttatcatcat cggaccatca aggtatctta cagcttctag ttgttcttta tttaaactct
     6361 ttgtgtataa atctgtcata ctaatttttg tgtaatttta aatatttaat taaaaaaaat
     6421 tttactttgt taaaaaaaaa ttaaatattt aatgcaaatt tacaataaat ttgatagaaa
     6481 actaattaaa aaaatttttt tgtaataata tttcctttgt atatttgcaa atgatgaaaa
     6541 aattaattta tgtaatattt atttgggtga ttgcattttt atacccaata tgtgtttatt
     6601 cacagataga tgacaatatt gatattaata tagattcttc agacatagct tttgcatttg
     6661 atagagctgt tatagaatta tattataata attatccatc taattttgaa cgtatatcta
     6721 ttgagcaaaa ttattataga agttttacag atgatgagtt agaagcttta ttaaatgata
     6781 gtctaatagc aaggagaata gcaataatga atgtaaattc tccttttgaa tatagatata
     6841 ataaagatgt aaagcagtgg atcaaatatt atctgcgtca tcgtcaattt ttaggtagaa
     6901 ttataggctt gagcgaatta tattttccta ttttcgaaga gacattcagt aaatacgaca
     6961 taccactaga gctaaaatat ctagcagtag tggaatctgc attaaatcct aaagctaaaa
     7021 gtagagctgg tgctatgggt ttatggcaat ttatgtatcg taccggatta ctttatgatc
     7081 tcaatgtaac ttcttatgta gatgatagat gtgatccata taaatcaaca gaagccgctg
     7141 ccaggcatat gcgagatttg tatgatattt ataatgattg ggcattggtt ttagcagcat
     7201 ataatgcagg tgcaggtagg ataaatagag ctgtattaaa ttctggtggt aaaacaaatt
     7261 attgggaaat tttaccttat ttacctaaag aaaccagaaa ttacgtacct gcatttattg
     7321 ctatttcata cattatgacc tattataaag aattaaatat cacaccatca cctcctaaat
     7381 ttaaagatgt tgaaatagat acagtaacta tcagatattc tacttcatta aaagctatta
     7441 gtgatttttt taatatttct tatgaagatt taaaatttct aaaccctcaa tttattaaag
     7501 atgttgtccc atctacagaa catcagatgt attgtattcg tatacctaga aaatatagaa
     7561 tgctatttta tagtaaagaa caagaatttt atgattcctt attagtcaag cgtgataaag
     7621 tggatactag ttattcatct agtgtaaaat cagaaactcc acctgcttct aatgttatct
     7681 accataaggt acaaaaaggc caaactctat ctagcattgc atcaaaatat cgagtaacgg
     7741 tttcagatat taaaaaatgg aacaatttaa aaagtaatat gatttatgta ggacaaaaat
     7801 taaaaattta tacaaataca ccagcaaaaa gtcctactag tacctctagc tctaccacag
     7861 cttctacttc gtcagaaaaa agcagtcaaa gtcaaactag ctctagctct aatgtaacta
     7921 caaaaactca aaatataact catatagtta aaagtggtga aaccctttct ggtattgcta
     7981 ataaatatgg agttactatt actgatatta aaaaatggaa taatctaact agtgacaaaa
     8041 tagtagtagg acaaaaatta atcattaatc ccactcaaaa atctattagc agttctggta
     8101 ataaagtaca tatggttaaa agtggagaaa ccctttctag tattgctttg aaatataata
     8161 caactgtaaa tgaaataatg aaaaaaaata atttaaaatc aacaaaaata gtagtaggac
     8221 aaaaattaat tataccatga aaaaatattt ttttgcaatt ttattattat tacttgtatc
     8281 cttgagctgt aaagagcaag ctaatatgcc agtttctatt ggtaaaatca atagtgtaat
     8341 tgtagtattg agaaacggag tgtctcaaac cccagcctcc aaaaccttag actctatact
     8401 tactcaagaa atctatggca tgccacaatc tgaatcatta ttcgacgtag ctcatgttac
     8461 agaagctaat ctgacagatt tattgaaaaa aaacagaaat ataatatacc tcatcatatc
     8521 tgatacagta aaaaaagatg gtatttttat gagtgaagat gttttcgcta caccacaaac
     8581 tattctattt atacgatctt cgcatcctga tagtgctaaa aaactgatcg aaaaatcaga
     8641 aaatcttatt ataaatactc taaaaaatac tgaatatcgt agaatatatg aaataatgtc
     8701 taaatcgcca gctgtagata ttaagaaaaa aataaatgaa aattttggta tagacttaca
     8761 attgatcaat cttttttttg aagctaaatc tactaaagat tttatgtggt taagattaga
     8821 aactaataat tatagccaag gacttatact tatgaaagaa ccattaatat ctgaaaatca
     8881 atttaatcca tctcacatta gaccatggat agatagcaca ctaaaattgc atattccagg
     8941 tcaattcgaa ggaacatata tgaaaataga tgatagcttc ataaagttaa aaatagatag
     9001 agtcgttata aatgatatcg aaagaatagt attcacaggt ttatggggag tacagggtga
     9061 ttttatgggt ggtccttttc gttcacatac ttttttgagc aaagataaaa ctcatttgat
     9121 caccgtctat gcttatgttt attatcctag tgaagaaaaa agaaatctta tgatggatct
     9181 cgaagccata ctctattcta tcaaatttgt ttagtttaat ccaacttata tcctaaaact
     9241 gataaataac gattgtttta caaataaaaa aaacgttgag atccaaaatt ttgaatctca
     9301 acgtcatgaa ttaaatgaaa ggtgttattt gagatcttta tacgtaaacc accaatcgta
     9361 gccttcgtat tcttttttac gttttggtat ttcagcgata tttttaggtg gtggtattat
     9421 acctttatct cctattagtt cgttattagg ccagttgtgc ggtagagata ctttgtattt
     9481 atcattcatt tgtagagctc gcaaagatct aatgatttca tctacatttc tgcctatttc
     9541 ttgggggtaa taaagtatga tacgaataat actattagga tctatgataa atactgctct
     9601 tgtagtttct gatcctttgt ttggatgtat catacccaga gcattagata cactaccaag
     9661 tggatcagct atgataggaa atggaacctc ttgttttaaa ttttcttcta tccattctac
     9721 ccatttgata tgagcaaata cttggtctac actgagtcca agtaattcag catttaattt
     9781 tttgaattca tcatatttct tagaaaaagc ataaaattca gttgtacata ctggtgtgaa
     9841 atcaccagga tgactaaaca gtatcaacca ttttccttta taatcatcag ggattacttt
     9901 gggaccatga gtggttaata ccttcatttg tggtaatggt tcacctatta aggggattct
     9961 tcctgtttcc ataatctttt attttttggg gttattgttt ttatattaat tattaaattt
    10021 atttcattat cattcactag atggggatat ttagttttga aatattcatt tagtatatta
    10081 tctaattctg catcttctaa atcttcgatt ttgccatctt cataataaat atgatgatgt
    10141 ttgtcctttt tataatcata tcttattata ttatcagatg tcatcaattt agttactata
    10201 ttattttttt caaattctga taaaatatta taaatcgatg acaatgaaat cgtaggcata
    10261 ataagcttga gggttgaata tatttcttct acacttggat gtgaagtggt attcatgagg
    10321 tattcgagta tttttatacg ttgtattgtg gcttttagat tgtgtttttt tagtactttt
    10381 agaggatcca ttttttcatg atttataaaa atttataagc aaaattaaat aattattttt
    10441 atataataac tattgtttat taagaataat tattatataa attgtaatta actacaaatc
    10501 aattaaatat gaatatatga taaatatcat attacttaat ctatcaatag attttttata
    10561 ttagacattt aactaataaa aataatatta tatttaaatt tgtttttctg agctaaagct
    10621 cattccttta tttttgttat tccccgactt aaaggtcggg gttactatct aggattttac
    10681 tttctgacta aacgtagcta ttgtggacgt atgattatat gctcatacga taatatatta
    10741 ggaggtaaat tgaccgtaca aaaatccatt ctttttaatc taaacattta attcgataaa
    10801 ttaattaata taataaaaaa aaccagatgt tttctgacat ctggtttttt tttactgaga
    10861 tagaacgaaa atttattcta taactaattt tttgatcaat ctagaacctt gagagtttag
    10921 agaaacaaaa tacatacctg attgtaaatt agaaatatca atagtagtat gatgtgaacc
    10981 agcttctttc atacctagat caatagatct aactaattga ccatttacgc taatgatatc
    11041 gatagaagta ttagatgcat tttgcagagc aaattgcact attagttctg gatcactagt
    11101 taaattagga aaggttaatt gcataccttg aaaataagag tttgagttta catcactacc
    11161 tttttctact attaagaata aagacatatt agcatctaaa ccttggcctc cactaaaagt
    11221 aagcccacaa ttgcaagcaa cccagtaata ttgagtagga tctttacaat attgtgaata
    11281 tcctaatcct aatccattac caaattcatc ccatactaaa gcagctgtat cactatttaa
    11341 acgcaaagat ttaaaatctg ctactataca aaaatcactg ctaatatata ctggatttgt
    11401 aaaagggaaa tattgccaaa ggctaggatc agcataacta gtagtatcta aatcattaat
    11461 atttacacta gatgaagcta atatatttcc actataataa tcaggaccag gaccataaga
    11521 agttggattg ccttgatcat caagtccagt tatagcttca tcttcaacag taggtaataa
    11581 gctagcagta agaacagaat tattatcctc tgatatatta tctttaatac ctatccaagc
    11641 caatatacct atagctgata ctgattcttc taaaacataa cattgagccc aataatcgat
    11701 atctgaacca tttactccac ctacatatcc gccactagtt aaaccatata tatgaagtcc
    11761 agaattagga tatccaaatt ctggtatact accaggtgtc catccaatgg tatctgtagt
    11821 agtttcattt gtatttttta caggcatcga agcatctaat gtctttttaa ctgatatttc
    11881 attttttgtc tcattggaca cttgcttttg tcccattaag agacctacac ttagcattaa
    11941 agctaatgat aaaattgttt ttaatccttt cataatcata tttttttaag ggtttgttat
    12001 gcaaaattaa ataatatttt ttaaataaca aattttttta aaaaaattat tttt
//
LOCUS       DAOPRN010000057        12025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_4482_length_12025_cov_109.263492, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000057 DAOPRN010000000
VERSION     DAOPRN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12025
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4482_length_12025_cov_109.263492"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..819
                     /locus_tag="PKI65_06615"
     CDS             169..819
                     /locus_tag="PKI65_06615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOB78147.1"
                     /translation="MKSLLITFMIVLFSSLNYLYSQSVKIIIHTDYGDITAVLYDKTP
                     LHKDNFIKLVESNFYDSLLFHRVIPNFMIQGGDPMSKNARPGQILGSGGPDYTIPFEY
                     IPEYFHKRGAIAAARMPDNINPNKESNGSQFYIVQGRKLTIEELNMIEQRGYKFTEEQ
                     KKTYTTIGGSPHLDGEYTVFGEVVSGMQVVDKIANMQTDKNNRPLQDIRFAIEIVK"
     gene            855..1877
                     /gene="pheS"
                     /locus_tag="PKI65_06620"
     CDS             855..1877
                     /gene="pheS"
                     /locus_tag="PKI65_06620"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOB78148.1"
                     /translation="MQEKIEKIKQELANLEAENITELEKIRIYFLGKKGILTALFEEL
                     KLLQPEEKKIYGKQLNELKNQIQYFINSKKRELELKSAESQTKIDFTRPADIFHPSSL
                     HPINIVKNRIINIFARIGFDIAEDIEIEDDWHNFTALNFPTDHPARDMQDTFFIERDP
                     DIVLRTHTSSVQIRIMESQKPPIRYIFPGRVYRNEAISSRSHVFFHQVEGLYIDYNVS
                     FADLKDTLEYLAQSLFDPSTKIRLRPSYFPFTEPSAEMDISCNLCDGDGCSFCKNTGW
                     VEILGCGMVHPNVLNNCNIDPNIFSGFAFGLGIERITNLLFSINDLRLFSENDLNFLH
                     LFSTLE"
     gene            1941..2651
                     /locus_tag="PKI65_06625"
     CDS             1941..2651
                     /locus_tag="PKI65_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78149.1"
                     /translation="MDTNEVSKIFEKLGGTFLTPANKLAKKLENTQAIFLDWDGVFNS
                     GEKGVEFPSSYNEIDSLGINLLRFALYRLSDKIPFIGIITDQVNESAFQLAKREHFDA
                     VYFTFKYTREAVQHVNEQFNIKPSQIMFVMDDVLDVSAADLVGTSFLVRRSASPLFEL
                     YVRENEFVDYITANTGDRNAVREICELVMGLLGKYNEVVEARLKYDDVYEEFLELRDK
                     IESRFYHKIDRKIIETII"
     gene            2657..3460
                     /locus_tag="PKI65_06630"
     CDS             2657..3460
                     /locus_tag="PKI65_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOB78150.1"
                     /translation="MNFLLGLSPFLLVCLGGLLVWLGTSLGSATVFFMSTPKRKIIDV
                     SLGFAAGLMLAASFFSLLMPAIEIERAKGNLVWVAPTIGFTLGIVFIKIIDFTVPHLH
                     ALGRKPHKDGGKTSLKGTTLLALAMIIHNIPEGLAVGIAFSSIDSTSLVGLAGAVSLS
                     IGIAIQDFPEGLAVSFPLLKEGISKWKAFAIGQFSGFVEFLSAIIGFLAVYYISDILS
                     YALSFAAGAMIFVVIEELLPASQNSQHNELATLFTLVGFILMMLLDLAL"
     gene            3668..5656
                     /locus_tag="PKI65_06635"
     CDS             3668..5656
                     /locus_tag="PKI65_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB78151.1"
                     /translation="MNIQYLSKLIIIISFFSLFFNCNLKAQEHKEKSLDDAVQYLMMA
                     KYDSSIYLLNELLAETPDYLLAASLKAYIYIELEKNNDALAIINQNLERDPDNILTLA
                     LKTNILKLSSKKDEINDEIQNNLNKILSLSPKDYSDYIGLGYAHYINNDYKKANKYYN
                     IAIKLNPSNPLGYYFLGLAYKEAKNYKSSINVLNKAIELNHDFVLAYYERGNIYSYQG
                     KYADAINDFEKTIELNPNFANGYYKLGDIYYELDDQAKAIQNLTKAIEINPQKPYYYF
                     TRALIFYNDDNIPAAMDDLNKLLELNPEFQVAYYTRADIYNRNNEYEKALKDYNKAIE
                     LSPNSYLYNRRGLIYMNLGMYDSAIVDFSKAIELQPKFATAYYNRGYAYRMLQQYDQA
                     LNDMNMVVKLEPNDKYAYNIRGLCFYKKEQYDQALKDFNKTIKLDPNYKHAYLNRGMT
                     YYFMKQYEKALKDLTQAIKLDPEFLDALISRGMILNVYEQYNLAINDFNKVIELTPDE
                     IDGYFMRALNYRNMGNYEAALNDFNKCIEIDPSNSFLYYQRAITYRILNKYDEAINDI
                     SIAIDLEPEYDQAYNLLGNIYYDTGEFDTALIYYQKAIEINPKYIAPYYNIANIYLEK
                     GDYASAKHCYSKIIELDPNGLYGKEALNKLNSLQNMGY"
     gene            5689..7563
                     /locus_tag="PKI65_06640"
     CDS             5689..7563
                     /locus_tag="PKI65_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB78152.1"
                     /translation="MKKNIIVKVKLSLIFLMVVLFHMPLISQNYDVEIENGIKLIQSD
                     RYDSAIIIFDNVLLNDSNNIIALFWKSYALEYLNKDDDALKCYNKILEINPDFLPAKV
                     YKCSLEYKDKNKKVENCYKKAIAIKPNDYLDYIAHGLANFYLKNYDDALYNYNKAIEL
                     NPDFAYSYLLLANLYFEKGDYDLALENYNKTNELNPDYIRAYFMKAYICHEKNQYDES
                     IKNYKKIIEINDTITNSYHNIAIDYYNLNYNDSALFFLNKAIELDSNYLDSYLLRGDI
                     YLYQGDYDRAMEDYNKILEIDNKNIYAYIGISRIYHENWHYDTAIIYYSKIIEIDPND
                     PVAYINRGISYEELGHHDLALKDYKKAIKIDSKIPEIYLYRGIVYINTNQYDKAIKDF
                     TKAIKLDSTMLYAYNYRGYVYSLKDDFQNAIIDFSKAIRIAPDFAEAYKNRALVYLAL
                     NEYQLAINDCNKMIEHDSLNDYYYFTRALIYYYYNKNDLAINDFNTAIKLAPGVVDYY
                     FYRGLYYHELNILDLAEQDYNKCIEIEPAFYKPYVNRANIYYQKGEYDLALTDLNKVI
                     ELYPDNINAYYLRGNIFLNKGDYAMAKKDYKKLLSLDPKGVFGKYAKEKLNLLKTLGF
                     "
     gene            complement(7567..8787)
                     /locus_tag="PKI65_06645"
     CDS             complement(7567..8787)
                     /locus_tag="PKI65_06645"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOB78153.1"
                     /translation="MNTKKDDSYISKRAAELKESATLAMSQKTRELKSKGIDVINLSI
                     GEPDFFVPNFIKEAAKQAIDDNYSFYPPVAGYPELRQMISHKLKRDNKLDYEPSQVVV
                     SNGAKHAIANIFLAIINEGDEVLIPAPYWVSYPPLVQLSQGVPVYINSTFDNDFRITA
                     EDIERHITPKTKAFIFSSPNNPSGKVYSYEELKEWAEVFAKYPQILIISDEIYELINF
                     KDKHHSIAEFEEIRDRVIIVNGLSKGFAMTGWRVGYSVSPKNIAKTIEKIQGQMTSGI
                     NDIGQMAALKALSVDPEDCHELIEMRESFRKRRDFMLEALSEIPFMIPNNPDGAFYIF
                     PKITDLIGKSYEDTKINDSNDLAMYLLNTANVALTSGISFGSKECIRLSYATSKLQLE
                     KAAERIKKAINLLK"
     gene            complement(9051..9566)
                     /locus_tag="PKI65_06650"
     CDS             complement(9051..9566)
                     /locus_tag="PKI65_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOB78154.1"
                     /translation="MEILEIIKNRRSIRKFKEDPIAKDIIDKLVEALIWAPSAGNLQS
                     RKFYFVFNEQLKKELSKAALFQTFISKAPLVIIGCTNERISTRYGKRGRELYTICDVA
                     ASIQNLMLLAHSEGLGTCWVGAFSEKNVSKILELPKHLRPIAIVPVGYPAHIPNPPKR
                     CSKEDAIKFII"
     gene            complement(9610..10989)
                     /gene="mnmE"
                     /locus_tag="PKI65_06655"
     CDS             complement(9610..10989)
                     /gene="mnmE"
                     /locus_tag="PKI65_06655"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOB78155.1"
                     /translation="MYLTNTNTICAIATPPGIGAISMIRISGNETLETLKKITKREEF
                     SIRKAHYCEIFSNSGELLDDAVVIYYKSPHSYTGEDVAEIFIHGSSYIQKTLLETLMD
                     LDLRLAEPGEFTYRAFINGKMDLLQAEAVNDLVFSTNSKHHNIALNQLRGGFSKKLSQ
                     YRDELIRLTALLELEIDFSEEDVEFVSRDELKNIIINLIDEAQRLIDSYRNGNVVKRG
                     IPVTIIGKPNVGKSTLLNAILNDDKAIVSEIPGTTRDAIEDQFTYLGHTFRFIDTAGL
                     QKTVDKIENIGIERSYDKMREAYIILYIIDISKFDYFQFLGEIDELKTQIQNFDNKKM
                     IIVFNKIDLIERIPPHIFDLKDYEKVFISAKRKENIEVLLDKIVNSIKDDFDLETDTL
                     INIRHYKAFNDIKDSLSLALKDIEKNMPNDIIALDLRQSIDSIGRITGEIVTDDILDH
                     IFSNFCIGK"
     gene            complement(11223..11507)
                     /locus_tag="PKI65_06660"
     CDS             complement(11223..11507)
                     /locus_tag="PKI65_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOB78156.1"
                     /translation="MKKGIHPEGYKFVVFKDMSNDFMFLTRSTAQSKETIVWEDGNEY
                     PLIKVEISNTSHPFFTGKMKLVDTAGRVDKFKNRYKNYKSKIEEDLDSDK"
BASE COUNT         4302 a         1620 c         1736 g         4367 t
ORIGIN      
        1 atttcttatg tggtaagcta aaatttgatt acccacacta tccgttatag aaccatttaa
       61 ttataatact tttttacaaa ttgcaatttt ttttaatatg taaaatctaa tctttttttt
      121 aaattattta ctgcattaaa aaaattatta attttgctaa aaaaagatat gaaatcttta
      181 ttaattacat ttatgatagt attattttct tctttaaatt atctttattc tcaatctgtc
      241 aaaataatca ttcacacaga ttatggtgat attactgcgg ttttatatga caaaactcct
      301 ctacacaaag ataactttat taaactcgta gagagtaact tttatgatag tttactattt
      361 catagggtaa tacccaattt tatgattcaa ggaggagatc caatgagtaa aaatgctaga
      421 ccaggacaga tattgggaag tggaggtcct gattatacaa taccatttga gtatatacca
      481 gaatattttc ataaaagagg tgctatagct gcagcacgta tgccagacaa tattaatcca
      541 aataaagaaa gtaatggcag ccaattttat atcgttcaag gacgaaaatt aactatagaa
      601 gaattaaaca tgatagagca gagaggatat aaatttactg aagaacagaa aaaaacttat
      661 actaccatag gaggatcgcc acatctagat ggggaatata cagtttttgg agaggtagta
      721 tctggcatgc aagtagtaga taaaatagct aatatgcaaa ctgataaaaa taatagacca
      781 ttacaagaca ttagatttgc aatagaaatt gtaaaataaa aatgttttta tttttaagat
      841 atttataaat aatcatgcaa gaaaaaatag aaaaaataaa acaagagcta gccaatttag
      901 aagctgaaaa tataactgaa cttgaaaaaa taagaattta ttttttaggc aaaaaaggta
      961 tattgactgc actattcgaa gaattaaagc ttttacaacc agaagaaaaa aaaatatatg
     1021 gcaagcaatt aaatgaacta aaaaatcaaa tacaatattt cataaattct aaaaagagag
     1081 aattagagtt aaaatcagct gaatctcaga caaaaataga ctttacaagg cctgcagata
     1141 tatttcatcc ttctagtcta catccgatta atatcgttaa aaatagaatt ataaatatat
     1201 ttgcacgtat aggatttgat atagccgaag atatagagat agaggatgat tggcataatt
     1261 tcactgctct aaactttcct actgaccatc cagctcgtga tatgcaagac acctttttta
     1321 tagaaagaga tccagatata gtgttgcgca cacatacatc tagtgtacaa atcaggatta
     1381 tggaatcaca aaaaccgcct attcgttata tatttccagg cagagtatat agaaatgaag
     1441 ctatatcttc tagatctcat gttttttttc atcaagtcga aggtttgtac atagattata
     1501 atgtttcatt tgctgatttg aaagatactt tagaatatct cgctcagagt ctttttgatc
     1561 cttctactaa aatcagactt cgcccatcat atttcccatt tactgagccc tcagcagaga
     1621 tggatatatc atgtaatttg tgtgatggtg atggttgtag tttttgcaaa aatactggtt
     1681 gggtagaaat acttggttgt ggaatggtac atcctaatgt tttaaataat tgcaacatag
     1741 atccaaatat tttttctggt tttgcttttg gtctcggaat tgaacgcatt acaaatttat
     1801 tattttcgat taatgatctt agactctttt ctgaaaatga tctcaacttt ttgcatttat
     1861 tttctacatt agagtgattg tgattttctt tatatattgc tatatgaata aattttttat
     1921 aattttgaca aaaaattttt atggatacta atgaggtaag taaaatattt gaaaagcttg
     1981 gtggcacttt tttgacacca gctaataaat tagccaaaaa acttgaaaat actcaagcta
     2041 tttttttgga ttgggatggc gtttttaata gtggcgaaaa aggtgtagaa tttcctagtt
     2101 catataacga gattgatagt ttaggcatca atttattgcg tttcgctctg tatcgtttaa
     2161 gtgataaaat tccttttata ggtatcatta ctgatcaagt aaatgaatct gcttttcaat
     2221 tggctaaacg agaacatttc gatgctgtgt attttacttt taaatatact cgtgaggctg
     2281 tgcagcatgt aaatgaacaa tttaatatca aaccttctca gataatgttt gtaatggatg
     2341 atgtactaga tgtatcggct gcagatttag tgggtacttc ttttctagta cgtcgtagtg
     2401 ctagtccact attcgagtta tatgtcagag aaaatgaatt tgtagattat ataactgcta
     2461 atactggtga tagaaatgct gttagagaaa tctgtgaact tgtgatgggc ttgctaggta
     2521 aatataatga agtcgtagaa gctcgtttga aatatgatga tgtttatgaa gaatttttag
     2581 agttgagaga taagatagaa tctagattct atcacaagat agatcgtaaa attatcgaaa
     2641 ctattattta ataaaaatga attttctttt aggattgtca ccatttttac ttgtctgcct
     2701 tggcgggcta ttagtatggc taggcacctc gttaggttct gctactgtat tttttatgtc
     2761 tacacctaaa agaaagatta ttgatgtttc tttaggtttt gcagctggac taatgttggc
     2821 agctagtttt ttctctttat taatgccggc aatagaaata gaacgtgcca aggggaattt
     2881 agtttgggtg gctcctacca ttggatttac tcttggaatt gtctttatta aaattataga
     2941 ttttactgta ccacatttgc atgcattggg taggaaacca cataaagacg gtggtaaaac
     3001 ttctctaaaa ggtacaactt tactggcatt agcaatgatt attcataata ttcctgaagg
     3061 tttggcagta ggtatagctt tttcatctat agattcaact tctcttgtag ggttagctgg
     3121 tgcagtatca ctaagtatcg gaattgctat tcaagatttc cctgagggat tagcagtgtc
     3181 tttcccttta ttaaaagaag gtatttctaa atggaaagct tttgccattg gtcaattttc
     3241 tggttttgta gaattcttat ctgctattat aggtttttta gcagtttatt atataagtga
     3301 tatactttca tatgcgctct cattcgccgc aggagctatg atttttgttg taatcgaaga
     3361 gcttttacct gcttcgcaaa atagtcaaca taacgaatta gctactttgt ttacgttagt
     3421 gggtttcata ttaatgatgc tgctcgattt agcattataa ttttttattt attaaaattg
     3481 taagtgcata taccgataag ctattctcgt cattgattta aatatattat tttggttgtt
     3541 taatataatg ataatatgca tatattttaa cattcgaatt aataaaccta attatgtatt
     3601 ttattaagta aaataaatta attaattttg ttttaaaatt ataagttaat aacataaata
     3661 atttattatg aatatacaat atctatcaaa attaattatt attatttcct ttttcagttt
     3721 attttttaat tgcaatttaa aagctcaaga gcataaagaa aaatcattag atgatgctgt
     3781 gcaatatcta atgatggcta aatatgatag ctctatttat ttattaaatg aattattagc
     3841 tgaaacccca gactatttat tagcagcttc tttaaaagct tacatttata ttgaattaga
     3901 gaaaaataat gatgcacttg ctatcataaa tcaaaatctc gaacgagatc ctgataatat
     3961 tttaacttta gctcttaaaa caaatatttt aaaattatca agcaaaaaag atgaaatcaa
     4021 tgatgaaatc caaaataatt taaacaaaat actttcatta tcaccaaaag attattctga
     4081 ttatattgga ttgggatatg cacattacat taataatgat tacaaaaagg caaataaata
     4141 ttataatata gctattaaac tcaacccatc taatcctctt ggatattatt ttctaggttt
     4201 agcatataaa gaagccaaaa attataaatc gtctattaat gttctcaata aagctataga
     4261 gttaaatcat gattttgttt tagcttatta tgaaagaggt aatatttata gttatcaagg
     4321 caaatatgct gatgctatta atgattttga aaaaactata gaattaaatc ccaattttgc
     4381 caatggatat tataaattag gcgatattta ttatgagtta gatgatcaag ctaaagcaat
     4441 acaaaatttg acaaaagcaa tagaaataaa tcctcaaaaa ccttattatt attttactag
     4501 agcattaata ttttataatg atgataatat acctgcagct atggatgatc tgaataaact
     4561 tttagaactt aatcccgaat ttcaagtagc atattatact cgagccgata tttataatag
     4621 aaataatgaa tatgaaaaag cattaaaaga ttataataaa gctattgaat tatcaccaaa
     4681 ttcttattta tataatcgta gaggtttaat ttacatgaat ttaggtatgt atgatagtgc
     4741 tatagtagat tttagtaaag ccatagaact gcaaccaaaa tttgctactg cttattataa
     4801 tcgtggttat gcttatagga tgttacaaca atatgatcaa gcactgaatg atatgaatat
     4861 ggtcgtcaaa ttagaaccta atgataaata tgcatataat attcgtggac tttgttttta
     4921 taaaaaagaa caatatgatc aagcactgaa agattttaat aagaccatca aattagatcc
     4981 taattacaaa catgcttacc taaatagagg aatgacatat tattttatga aacaatatga
     5041 aaaggcactg aaagatttaa ctcaagctat caaattagat cctgaattct tagatgcttt
     5101 gatcagtaga ggtatgatat taaatgtata cgagcaatac aatttagcaa ttaatgattt
     5161 taataaagta atagaattga caccagatga gatagatggg tattttatga gagctctaaa
     5221 ctatagaaat atgggtaatt atgaagctgc attgaatgat ttcaataaat gcatcgaaat
     5281 agacccttct aattctttct tatattacca gagagccatt acttatcgta tattaaataa
     5341 gtatgatgag gctattaatg atatatctat cgcgatcgat ttagaaccag aatatgatca
     5401 agcatataat ttattaggta atatctatta cgatacaggt gaatttgaca cagctttgat
     5461 ttattatcaa aaagcaatag aaataaatcc caaatatata gcaccttatt ataatattgc
     5521 taatatttat ttagaaaaag gtgattatgc ttctgctaaa cattgttatt ctaaaataat
     5581 tgaattggat cctaatggct tatatggcaa agaagcgttg aataaattaa attcactcca
     5641 aaatatgggc tattgatttt tttttaacat ttaaaaaatt atatacagat gaaaaaaaat
     5701 atcatagtaa aagtaaagtt gtcactaata tttttaatgg tcgttttatt tcatatgcct
     5761 ctaatcagtc aaaattatga tgtagaaatt gaaaatggga taaaactgat acagtctgat
     5821 agatatgact ctgctattat aatatttgat aatgttttgc taaatgattc aaataatata
     5881 atagcattat tctggaaatc ttatgcatta gaatatttaa ataaagatga tgatgctctc
     5941 aaatgttata ataaaatttt agaaataaat ccagattttt taccagctaa agtttataaa
     6001 tgtagcctag agtataaaga caaaaataaa aaggtagaaa actgttataa aaaagcaatt
     6061 gctattaagc caaatgatta tttagattat atagcacatg gattggcaaa tttttattta
     6121 aaaaattatg atgatgctct atataactat aataaagcta ttgagctaaa tcctgacttt
     6181 gcctattctt acctgttact agcaaattta tatttcgaaa aaggtgatta tgacttagcc
     6241 ttagaaaatt ataataaaac taatgaatta aatcccgatt atatcagagc atattttatg
     6301 aaagcatata tttgtcatga aaaaaatcaa tatgatgaat ctattaaaaa ctataaaaaa
     6361 attatagaaa ttaatgatac tatcacaaat tcatatcata atatcgctat tgattactat
     6421 aatttaaatt ataatgattc tgcattgttt tttttaaata aggctatcga attggattct
     6481 aattatttag attcatattt attaagggga gatatatatc tgtaccaagg tgattatgat
     6541 cgagctatgg aagattataa taaaatttta gaaatagata ataaaaatat ttatgcatat
     6601 attggaatta gtcggatata tcatgaaaat tggcattatg atacagctat aatttattat
     6661 tcaaaaatca tcgaaataga tcctaatgat cctgtagcat acataaatag aggaatttct
     6721 tatgaagaat taggacacca cgatttagct cttaaagatt ataaaaaggc tataaaaata
     6781 gattctaaaa ttccagaaat ctatttatat cgaggtattg tttatattaa tactaatcaa
     6841 tatgataagg ctattaaaga ttttactaaa gctattaagt tagattctac tatgttatac
     6901 gcatataatt atcgaggtta cgtgtattct ttgaaagatg attttcaaaa tgctatcata
     6961 gattttagta aagcaataag aatagcacct gattttgctg aagcatataa aaatagagct
     7021 cttgtatatt tagcactaaa tgaatatcaa ttagccataa atgattgtaa taaaatgata
     7081 gaacatgatt ctttaaacga ttattactat tttacaagag cattaattta ttattattat
     7141 aataaaaatg atttagctat taatgacttt aatactgcta ttaaacttgc acctggtgtg
     7201 gtagattatt atttttatcg aggtttgtat tatcatgaat taaatatatt agatttggct
     7261 gagcaagatt ataataaatg tatagaaata gaacctgcat tttacaaacc ttacgtaaat
     7321 agagcaaata tttattatca aaaaggagaa tacgatttag ctcttacaga tttgaataaa
     7381 gttattgaat tatatccaga caatattaat gcatattatc tgcgtggtaa tatatttttg
     7441 aataagggag attatgctat ggctaaaaaa gattacaaaa aactactatc tctcgatcct
     7501 aagggtgtgt ttggtaagta tgcaaaagaa aaacttaatc tattaaaaac attaggtttt
     7561 tgatatttat ttaagtaaat tgatagcttt tttaattctt tcggcggctt tttctaattg
     7621 caacttagaa gtagcatagc tcaaccttat acattcttta gaaccaaaac ttatgccaga
     7681 agtcagagct acattagcag tatttaaaag atacatagct aaatcattac tatcatttat
     7741 tttagtatct tcatagcttt tgccaataag atctgttatt ttgggaaaaa tataaaaagc
     7801 accatctgga ttattaggaa tcataaaggg aatttctgaa agtgcttcta gcataaagtc
     7861 tctacgctta cgaaacgatt ctctcatttc tattaattca tgacagtctt caggatctac
     7921 agacaatgct tttagagcag ccatctggcc gatatcattt atgccgctag tcatttgtcc
     7981 ttgaattttt tcgatagttt tagctatatt tttaggtgac acactatatc ctactctcca
     8041 tccagtcatt gcaaaacctt tagacaagcc gttaacaatt atcactctat cacgaatctc
     8101 ttcgaattca gctatactgt gatgcttgtc tttgaaattt atcagttcat atatctcatc
     8161 tgatattatt aaaatttgtg gatatttagc aaaaacttca gcccattctt ttaactcttc
     8221 ataagaataa acttttccag aaggattatt aggagaacta aatataaatg ctttagtctt
     8281 tggtgtgatg tgacgctcaa tatcttctgc ggttattcta aagtcattat caaaagtact
     8341 atttatgtat acaggaactc cttgagacag ctgaacaagt ggggggtaag acacccaata
     8401 tggtgcagga atcagtactt cgtcaccttc atttataata gctaaaaata tattagcaat
     8461 tgcgtgcttg gccccattag atactactac ttgagaaggt tcataatcga gtttgttgtc
     8521 tctttttaat ttatgcgata tcatttggcg tagttctgga tatccggcca ctggcggata
     8581 aaaggaataa ttgtcatcta tggcttgctt agcagcttct ttaataaaat ttgggacaaa
     8641 aaaatcaggc tctccgatac ttaaatttat aacatcaatg cctttacttt ttaattcacg
     8701 cgtcttttga ctcatagcta aagtagctga ttcttttaat tctgcagctc gcttagatat
     8761 gtaactatca tctttttttg tattcatttt tttgtcaaaa ttaataaaag attttatctt
     8821 aggtgtaaat attttaaaaa atttagaatt aattttcaat tattacgtta aagatatata
     8881 aattattatt taagaaaata taattttgtt taatctaaat aatatttgca aaactaatgc
     8941 tatactagat aattagacat taaagatata taaattattt aattattaaa taatttataa
     9001 tcagttgtat taatccgaaa tgcaattaaa tttattatta taaaaaaaaa ttaaataata
     9061 aatttaattg catcctcttt tgagcaacgc tttggtggat taggaatatg cgcaggataa
     9121 ccgacaggaa cgatggcaat aggcctcaaa tgcttaggca actctaatat tttagataca
     9181 tttttctctg aaaatgctcc tacccagcaa gtacctaacc cttcggaatg agctaaaagc
     9241 attagatttt ggatagatgc tgctacatca catatagtat atagctctct acctctctta
     9301 ccatatcttg tagaaattct ttcattagta catccaatga ttactaatgg tgctttagaa
     9361 ataaaagttt gaaaaagagc tgctttgctt agctcttttt taagctgttc attaaataca
     9421 aaataaaatt tacgagattg taaattacct gccgatggag cccagatcag agcttctact
     9481 aatttatcaa ttatatcttt agcgatagga tcctctttaa attttcttat gcttctacga
     9541 tttttaatta tttctaatat ttccatattt tttactaata tcaaaaaaac caatctattt
     9601 atataaatat tatttgccaa tacaaaaatt gctaaatatg tgatctagta tatcatcagt
     9661 tactatttcg ccagttatcc tacctatact atctatgctt tgccttagat ctaatgctat
     9721 aatatcattt ggcatatttt tttctatatc tttgagagct aagcttaggc tatcttttat
     9781 atcattaaaa gctttataat gcctaatatt tatcaatgta tctgtttcta agtcaaaatc
     9841 atctttgata gagtttacaa ttttatccaa taatacttcg atattttctt ttcttttagc
     9901 agagataaat actttttcat aatcttttaa atcaaatatg tgcggaggta ttcgctctat
     9961 caaatcaatt ttattaaaaa caataatcat ttttttgtta tcaaaatttt gtatttgagt
    10021 tttgagctca tctatctcgc ctaaaaattg aaaatagtca aatttagaaa tgtctattat
    10081 atataaaatt atataagctt ctctcatctt atcataagag cgttcaatac caatattttc
    10141 aattttatct actgtctttt gtaaacctgc tgtatcaata aatctaaaag tatgccctaa
    10201 ataagtaaat tggtcctcta tggcatctcg agtagtacca ggtatttcac ttactattgc
    10261 tttgtcatca tttagtatag cattcaataa agtagattta cctacatttg gcttgccaat
    10321 aatagttact ggaataccac gtttcacaac atttccattt ctatatgaat ctatgagacg
    10381 ctgtgcttca tcgatgaggt taattataat attttttaat tcatcacgag agacaaattc
    10441 aacatcctct tcagagaaat caatttctaa ttcgagtaat gctgttaatc gaattaattc
    10501 atctctatat tgacttaatt ttttagaaaa accacctcta agctgattta atgctatatt
    10561 gtgatgtttg ctattagtag aaaagaccag atcatttaca gcttctgctt gtaataaatc
    10621 cattttgcca ttaataaaag ctctataagt gaactcacca ggctctgcta accttaaatc
    10681 taggtccata agagtttcta ataatgtttt ttgaatatac gatgacccat gaataaatat
    10741 ttctgctaca tcttctccag tatatgaatg aggagattta taatagatta caacagcatc
    10801 atctaatagc tctccactat tagaaaaaat ctcacagtaa tgagcttttc taatagaaaa
    10861 ttcttctctc tttgtaatct tttttaaagt ttctaaagtt tcattaccac taattcgaat
    10921 catagatata gctccaattc cagggggtgt agctatagca catattgtat ttgtgtttgt
    10981 taaatacatt tgtctttttt ttctttaata caaatataac acgaaatttt aatattttta
    11041 taaataaata ttatctaaga tcaataagta tttagtacgt atatcaaata aaaaaattat
    11101 aatttaaaat aaacctgaga ttttattaac tatcgagata ataaaattat taatattata
    11161 tcacgaatca atgtaatact aatgatttgt ttcaaagata aagtaaatag agaattaaat
    11221 ttttatttat cactatctaa gtcttcttct attttagatt tatagttttt atatctattt
    11281 ttaaatttat ctacacgacc agcagtatca accaatttca tttttccagt gaaaaatgga
    11341 tgtgatgtgt ttgagatctc tacttttatt aatgggtatt cattgccatc ttcccaaaca
    11401 atagtttctt ttgactgagc tgtgctgcga gtaagaaaca taaaatcatt agacatatct
    11461 ttgaaaacca caaacttata accttctgga tgaattcctt ttttcatttt tattctaatt
    11521 ttttgcaaaa ttataaaatt atttttaaat aaaaaagtta taattgataa ttagataact
    11581 ataattacac ataaatattt taatttattt attactgtta ggtatattat gaatataaaa
    11641 ataaaatatg taagtaaatg aaatgtaaca gcacactggt tattttttat agtatcaata
    11701 tttattttaa agtaaataat tatagaactt cattgagggc gatttgttgg aaaaggcttc
    11761 ctgcaaattc atctttgttt tactttacgt agtttatagc ataattaaat aattagacgc
    11821 agtatataca gtgtacaggt agtcgataag catatgaacg atttcaaggt ttggttacgt
    11881 cgatttagag gtgtggctac caaatactta cagagctatc tgatgtggtt tgtaatcaca
    11941 cataaatact taataagtga gatagaatca gacattggtc ggcttttaaa cctcgcatcc
    12001 tcggacagat gggcgtggga tgagt
//
LOCUS       DAOPRN010000058        12013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_4489_length_12013_cov_101.567152, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000058 DAOPRN010000000
VERSION     DAOPRN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12013
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4489_length_12013_cov_101.567152"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(410..1099)
                     /gene="truB"
                     /locus_tag="PKI65_06665"
     CDS             complement(410..1099)
                     /gene="truB"
                     /locus_tag="PKI65_06665"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOB78157.1"
                     /translation="MIFDFEKGELLLIDKPLDWTSFDVVNYIRKTITKYLKHPIKVGH
                     AGTLDPKATGLLIVLTGKKTKLMNELLNLNKEYTGTMKLGETTPSYDRELPVNNYYPI
                     DHITYQDIYNLAKKFIGRQEQYPPVYSAIRIEGKRAYEYARENKDVVINPRFVTIHEF
                     EITNIELPFIDFRLCTSSGTYIRSIVRDFGFLLESGAVLENLRRTKIGDYSVNDANDP
                     RLLDNLLEQYF"
     gene            complement(1256..2032)
                     /locus_tag="PKI65_06670"
     CDS             complement(1256..2032)
                     /locus_tag="PKI65_06670"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOB78158.1"
                     /translation="MTWLEAIILSIVEGLTEFLPISSTGHLIITTYLLGITKTEFINV
                     FIIAIQFGAILSVLLLYFKKFTESWRFYYYLLIAFLPAAVVGFFVESYIDKLLGSEWV
                     VGIALFLGGIALMYFDKWFQNPSPDQQLTEKKAFKIGLLQMLAFIPGVSRSAATIFGG
                     LVVGLDRKNATEFSFFLAVPTILGAAVYKVYKHYDVLFQANNASLLIVGNIIAFITAA
                     IAIRFFINYIVKYGFKAFGIYRIIVGGLLMILLACNQFFV"
     gene            complement(2029..2280)
                     /locus_tag="PKI65_06675"
     CDS             complement(2029..2280)
                     /locus_tag="PKI65_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOB78159.1"
                     /translation="MKKSKKEKDIQLIFSKKNYILMIIGLLLMGLGYVLMIGGGSADP
                     NVFSEEIFSFRRITLAPILILLGIVVEIFAILLNFDKKV"
     gene            complement(2373..3362)
                     /locus_tag="PKI65_06680"
     CDS             complement(2373..3362)
                     /locus_tag="PKI65_06680"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOB78160.1"
                     /translation="MNIINELREIESEIFDDNLDFDSIALRLFELHYENNEVYHKYCK
                     IIGKNPNKISNIDEIPLLPVDIFKNQKVILRDLPAERIFETSGTIVDKKGKHYVPFLR
                     VYDKSLLVSFEKFFGSPSQYRFLFLLPNYLEQPNSSLIYMASILARNAIEAVFIKWDY
                     KSVFEIIKKWNKEKSKIIIWGVSFALLEFTEKYQIHLDNAIIIETGGMKGKREEMTRD
                     ELHEKLKNAFSIENIASEYGMTELLSQAYAIKNGIFYSPKWMKIVIKDRYNHLLSIPN
                     MTNGKIGIIDLANLYSMPFILTDDIGIKYLDGSFEVIGRFDYSVLRGCNLLYF"
     gene            3409..3918
                     /locus_tag="PKI65_06685"
     CDS             3409..3918
                     /locus_tag="PKI65_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78161.1"
                     /translation="MKIKNIKIIFLLSIFLIFFGGCIENIKYPDKPEIEFKDFQIIRS
                     KDNKDSLGILIFSFTDGDGDIGLDEKDTFPPFHPTSIYYYNFYLHFIEYNDQGDSIRQ
                     LEPPYTARIPKTDRYGESIRGEIYVELDIYFLPIVLQYNNVRMKFYIYDRALNKSNIE
                     YTPIYKFSF"
     gene            3987..4850
                     /gene="rfbA"
                     /locus_tag="PKI65_06690"
     CDS             3987..4850
                     /gene="rfbA"
                     /locus_tag="PKI65_06690"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOB78162.1"
                     /translation="MKGIILAGGSGTRLYPITLAISKQILPVYDKPMVYYPLSTLMMA
                     GIRDILIISTPHDLPSFKKLLKDGSQWGCSFSYAEQPVPNGLAQAFVIGESFIGNDKV
                     CLILGDNLFFADGFTQLLQSCNNPDGAIIFAYHVSDPERYGIVEFDKNLNVISIEEKP
                     KNPKSNYAIPGLYFYDNDVVEIAKSLKPSARGEYEITDVNKRYLELKKLKVKILSRGT
                     AWLDTGTFESLLQASQFVHVIEKRQNLKIGCIEEVAYRMGYINEMQLRNLAEPLLNSG
                     YGEYLLSILEN"
     gene            4864..5808
                     /locus_tag="PKI65_06695"
     CDS             4864..5808
                     /locus_tag="PKI65_06695"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOB78163.1"
                     /translation="MKYVLVTGGAGFIGSSLVEKLIKNPDFFVIVIDNLSTGSIDKLP
                     RNDSDRLLFIKLDVNNYDDISSVFFNYPIDYVFHYAAIVGVQRTLRQPLKVLEDIQGI
                     ENICRLAKNTNVKRVFYSSSSEVYGESVDFPQNEDTTPLNARLPYAAVKNLGEIYLKT
                     YNNEFGLHYTIFRFFNTYGPKQNSDFVISKFIRQAFKEKQITIYGDGMQTRTFCYIAD
                     NVDATYNHLLQCVENPNMLPQIINIGNDNEITILELAQTIKKLTNSNAEITFLPPLQE
                     GDMKRRLPDITKMKKLLNRPLTPLHEGIKNILDNNIFY"
     gene            5928..6965
                     /locus_tag="PKI65_06700"
     CDS             5928..6965
                     /locus_tag="PKI65_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOB78164.1"
                     /translation="MNKNRRKVLLIYTGGTIGMVFDEKLKTLVPVNFDKLNSYISYID
                     QMPIDIRHISFENPIDSSNILPKHWVKLVDIIEKHYIEYDGFLILHGTDTMAYTASAL
                     SFLLENLNKPVILTGSQLPLDMIRTDGRDNLIAALEIASAYKDDLPIVPEVAIYFENK
                     LFRGNRTSKFNAENFDAFYSGNYPVLAQVGVHIKYYTEYINKPTNKPLKVYKKLDNSV
                     GLIKIYPGMPIKILEDILYDKDLKLVILETFGTGNAPTYDEFLKPIKKAIEDKKVILN
                     ITQCKAGKVELGRYATSLSLENLGVISGADMTVESAITKSMFVLGNFTDKNQIIRLLQ
                     TSLRGEMTDNE"
     gene            7171..7542
                     /locus_tag="PKI65_06705"
     CDS             7171..7542
                     /locus_tag="PKI65_06705"
                     /inference="COORDINATES: protein motif:HMM:NF040945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCC motif membrane protein"
                     /protein_id="HOB78165.1"
                     /translation="MENQEFSVKNDQSNVTKYQSLPGAQSSFILGILSVVFTLTTVIV
                     GIILGIIAVVEANKAMKAYEINPSLYTKSEYSNANVGKILGWISIGLGSALFLFIILI
                     IIFAVSLAIPFSACGVISSFF"
     gene            7553..9121
                     /locus_tag="PKI65_06710"
     CDS             7553..9121
                     /locus_tag="PKI65_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOB78166.1"
                     /translation="MKVLSIEDIRKADAYTMANEPITSIDLMERAATNCFAFILKHFF
                     DNDDFVIFAGPGNNGGDGLVIARRLASVGYRVKVFICEFSKSYSEDCLINLKRLPKDV
                     EIIKLKNKDDIFHLSKINEKSIIIDALLGSGITRPPDNWLADLIKSINSLSNFKIAID
                     MPSGLFSDKSSKAHFDRVIAADITLTFEVPRLAFFMPENYPYIGVWTVLPIGLDYDFI
                     DKCNPLAIIIDGELIKQKLKKREPKAYKNLFGHVCIAGGSPGKYGAALLSTIGALKSG
                     AGLVTSIVPRDLISVYLSRVPEALTVLIDNPFVNDFSLINDYDVACIGPGLGNDPATA
                     QAFHNYLIDCKKPTVLDADALNILSQNIEWCELLNENFILTPHPGELKRLLGTWRDDF
                     EKIDKAKDFCNTYNCNLIIKEHNTKIINPQGDLFINITGNDTLSKGGSGDILAGLLAG
                     LLAQKYSPTDASIIAVCLHGAAGEWCGKRYSHHATSISQLTDGIENIFLNWEQNDFSM
                     ISINTFTYEGYLII"
     gene            9221..10633
                     /locus_tag="PKI65_06715"
     CDS             9221..10633
                     /locus_tag="PKI65_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOB78167.1"
                     /translation="MTDKIVNSLRESKTARWTALILVAFTMLTGYFIADVMSPLKPLV
                     EAELKWSSTEYGIFTGAYGWINVFCLMLIIGGIILDVAGIRFTCMLSASIMLIGTAIK
                     YWAISTPALNNYVINFLFWPMRGQVFWASIGFAIFGVGVEMAGITVSKTIVKWFKGKE
                     MALAMGLEMATARLGSALALMVSPVIAKSMGGVSKPILFGLMLLCIGFITFFIYIFMD
                     KRLDESEGITKENKPESEFKFKSIGRILSNKAWWYIAIMCVLFYSAVFPFLKYASDLM
                     VNKFNVDPSIAGVIPGILPFGTILLTPIFGNIYDRIGKGVTIMVIGAFLITFVHLIFS
                     IPGLHSVVVAIILVILLGIGFSLVPSAMWPSVAKIIPESELGTAYALIFWIQNWGLMG
                     VPMLIGWVLDKYCITGQVMRDGVLVNSYDYTLPMLIFAGLSAISIIFAFLLLAENKKK
                     KYSLQLPNIEEKIKEKEAND"
     gene            10694..11167
                     /locus_tag="PKI65_06720"
     CDS             10694..11167
                     /locus_tag="PKI65_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOB78168.1"
                     /translation="MQLPQLLFPVDDLKIIEKGDILYIYDPIRYQYLKCTPEEYVRQA
                     LISHLINNLSYPKELLAVEKQIFVNKLSRRFDLVVFDRDIYPLILIEIKAPNVKINDK
                     TLLQLAQYNYIINAKHWVLTNGLNVLFMTYDKENNKIDFKSQIPTWYELGKSDNK"
     gene            complement(11246..11692)
                     /locus_tag="PKI65_06725"
     CDS             complement(11246..11692)
                     /locus_tag="PKI65_06725"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HOB78169.1"
                     /translation="MLFRKQNIVLFIAWLLLILLFFIPFIRLISPSNIPITIYDTKAP
                     LQPIGWLYALIVVHILLILLLPITIYRIKNLIHQLRLNAVAFFLNVLLLLGILLIPDV
                     WVKHGILYRYDYIGVLIPIISFIMIILANKYIRSDIQKIRSANRLR"
BASE COUNT         4330 a         1571 c         1808 g         4304 t
ORIGIN      
        1 taaaatacca ttgaaattct aacgtctgac cacttattct ctttttatct aaacacaaaa
       61 tgtgttattt tgttttttta aaaagcagca aatattttta tagaactaat taatcagata
      121 atattagtat agcatttgta tagctagaaa ttcagaaagc ttaagaaagc ttatcagagc
      181 aatcatgatt tagttacatc aatattttta atttatatta taataataaa actattttga
      241 ggttctataa cattttatgt aggtaaagtt attaaaacat aaatataaaa cactaattaa
      301 atcgcatatt tttcacaagt cattccgaca gacattaatt tagaatattt ctatgcacta
      361 gcgttttagc tatgttgaat agtaatatca aggaaaaatc aaaatctaac taaaaatatt
      421 gttctaataa attatctaat aatcttgggt cgttagcatc atttactgaa taatcgccta
      481 ttttagtcct tcttaaattt tctaaaacag ctccactttc taataaaaag ccaaaatctc
      541 gtacgataga tctgatgtaa gtgccactgg aagtgcaaag gcgaaaatct ataaagggta
      601 attcgatatt tgttatttca aattcatgaa tagtaacaaa tcttggattt atcactacat
      661 ctttattttc tctagcatac tcgtacgccc ttttgccttc tattctaatt gcagaatata
      721 ctggggggta ttgctcttgc ctaccaataa attttttagc taaattataa atatcttgat
      781 atgttatgtg atctataggg taataattat ttacaggcag ttctctatca tagctggggg
      841 tagtttcacc taatttcata gtacctgtat attctttatt caaatttagc aattcgttca
      901 ttaatttagt ttttttgcca gtcaaaacta tcaataaacc agtagctttg ggatctaatg
      961 tcccagcatg tcccaccttg ataggatgct ttaaatattt tgtaatagtt tttctaatat
     1021 aatttactac atcaaaagat gtccaatcta gtggcttatc tattaataat aattcccctt
     1081 tttcgaagtc aaatatcata attttttttt aattttacat aattaaaaaa atctatatta
     1141 tttataaatt ttaatgatca aaattcaaat ttatcatgtt ggagactttt tatatttgaa
     1201 taaattagtt tgtagtgtaa gttttttatt ttcatttata atattaatta attcattata
     1261 caaaaaattg attacaagcc aaaagtatca tcagtaggcc ccctactatt atacgatata
     1321 taccaaaggc tttaaatcca tattttacaa tataatttat aaaaaatcta atagctattg
     1381 ccgcggtaat aaaagcgata atattgccaa ctattaataa tgaagcatta ttagcttgaa
     1441 ataagacatc ataatgctta taaactttat acacagcagc accaagtata gtgggtacag
     1501 cgaggaaaaa agaaaattct gtagcatttt ttctgtctag gcctactact aaacctccaa
     1561 atatagttgc agcagatcgt gacactccag gtatgaaagc aagcatctgc aataaaccaa
     1621 tcttaaaagc tttcttttct gtcaattgct gatctggtga tggattttga aaccatttat
     1681 caaaatacat caaagctatc ccccctaaaa ataatgctat acctactacc cactcgctac
     1741 ctagtaattt atctatataa ctttctacaa aaaaacctac taccgctgca ggcaaaaaag
     1801 ctattagtaa ataataataa aaacgccagc tctcagtgaa ttttttaaaa taaagaagca
     1861 acacactcaa aatagctcca aattgtatcg ctatgataaa aacatttata aattcagtct
     1921 tagtaatgcc taataagtaa gttgtaataa ttaggtgacc tgtagagctg ataggcaaaa
     1981 actctgtaag tccctctaca atagaaagta ttatagcttc taaccaggtc atactttttt
     2041 atcaaagttt agtaaaattg caaatatctc tactactatg cctaaaagta taagtatcgg
     2101 agctaaagtg attctacgaa aagaaaaaat ctcttcacta aatacatttg gatcagcaga
     2161 tccaccacct atcatcagca cataccctaa gcccattaat aatagaccta ttatcattaa
     2221 gatataattt tttttactaa aaattagttg tatgtccttt tccttttttg attttttcat
     2281 tttttctgca aaaataataa ttattttaat tgggtcaata tgattttttt ttattagttg
     2341 aagataattt attagttttt ttaaatattt tttcaaaaat aaagcaaatt acatccacgc
     2401 aaaactgaat aatcgaaacg tcctattact tcaaaagaac catctaaata tttgatgcca
     2461 atatcatctg ttaaaataaa aggcatagaa tatagatttg ctaaatcgat aattcctatt
     2521 ttgccatttg tcatattcgg tatcgaaagc aaatgattat atctatcttt aataacaatt
     2581 ttcatccatt taggagaata aaaaattcca tttttaattg cataagcttg agatagtaat
     2641 tcagtcatac catattcact agctatgttt tcgatgctaa aagcattttt taatttttca
     2701 tgcaactcat ctctagtcat ctcttcacgt tttcctttca taccacctgt ttcgatgata
     2761 atagcattat cgaggtgtat ttgatatttt tctgtaaatt ctaataaagc aaagcttacc
     2821 ccccagatta taatttttga tttttcttta ttccattttt taattatttc aaatacagat
     2881 ttataatccc atttgataaa aactgcttca attgcatttc tagccagtat actggccata
     2941 tatataagtg atgaattggg ttgttctaaa taatttggta aaagaaataa aaatctatat
     3001 tgtgatggag atccaaaaaa tttttcaaag gatactaaaa gactcttgtc atagactcta
     3061 agaaatggaa cataatgctt tccctttttg tcaacaatcg tcccgctagt ctcaaatatt
     3121 ctttcagcag gtaaatctct tagaataact ttttgatttt taaaaatatc tactggtaat
     3181 aatggaatct catctatgtt tgatatttta ttgggatttt taccaattat tttacagtat
     3241 ttatgataaa cttcattatt ttcataatgc aattcaaata atctcaatgc tattgaatca
     3301 aaatcaagat tatcatcaaa aatttctgat tctatttctc ttagttcatt aattatattc
     3361 atcttaaatt catcaaaaat ttgtaatttt acacaaattt aataatttat gaaaataaaa
     3421 aatataaaaa ttatattttt gctttcaatc tttttaatat tttttggtgg ttgcatagaa
     3481 aacataaaat atccagataa accagaaata gaatttaaag attttcaaat tataagatca
     3541 aaggataata aagatagttt aggaatatta attttttctt ttactgatgg agatggtgat
     3601 ataggtttag atgaaaaaga tacatttcca ccatttcatc ctacaagcat atattattat
     3661 aatttttatt tacattttat tgagtataat gatcaaggtg atagcataag gcaattagaa
     3721 cccccttata cagctagaat cccaaaaaca gatagatatg gagagagtat aaggggtgag
     3781 atttatgtag agctagatat ttattttttg ccaatcgtgc tacaatataa taatgtcaga
     3841 atgaaatttt acatatatga tagagcatta aacaaaagca atatagagta tactcctatt
     3901 tataagtttt cgttttaatt attcttatga tagtaaaaat ctttatattt aattttctta
     3961 tttatttttg taaaaaataa tattttatga aaggaataat attagctggt ggttcaggta
     4021 cgaggttgta tcctattaca cttgctatta gtaaacaaat tttacccgta tatgataaac
     4081 ctatggtata ttatccatta tctactttga tgatggctgg tattagggat atacttatca
     4141 tttctacacc tcacgattta ccatctttta aaaaactttt aaaagatggc tctcagtggg
     4201 ggtgttcgtt ttcatatgca gaacaacctg ttcctaatgg attagctcaa gcttttgtaa
     4261 taggtgaatc tttcataggt aatgataaag tgtgtctgat attaggtgat aatttgtttt
     4321 ttgcagatgg ttttacacaa cttttgcaga gttgtaataa tccagatggt gctattattt
     4381 ttgcatatca tgtctctgat cccgaaagat atggtatagt agaattcgac aaaaatctaa
     4441 acgttatatc tatagaagaa aaacccaaaa acccgaaatc aaattatgct attcctggct
     4501 tatatttcta tgataatgat gtagtagaaa tagccaaatc tctcaaacct agtgctagag
     4561 gcgaatatga aataacggat gtaaataaac gctatttaga attaaaaaaa ttaaaagtta
     4621 aaattctctc aagaggcaca gcttggttag atacaggcac tttcgaaagt cttttgcaag
     4681 cttcgcaatt tgtccacgta attgagaaaa gacaaaatct taagattggt tgtatcgaag
     4741 aggtagctta tagaatgggg tatattaatg aaatgcaatt aagaaatcta gccgaacctt
     4801 tgttaaatag tggctatggt gaatatttat tatctatttt agaaaattag aaacttttag
     4861 attatgaaat atgttttggt tacaggtggg gccggattta taggtagctc attagtagaa
     4921 aaattgataa aaaatcctga tttttttgtt atcgttattg ataatttgtc cactgggtcc
     4981 attgataaac taccacgaaa tgactcagat agacttttat ttattaaatt agatgtaaat
     5041 aattatgatg acatcagctc tgtatttttt aattacccca tagattatgt atttcattat
     5101 gccgctatag taggtgtaca gagaacgttg aggcagccac ttaaagtatt agaagatata
     5161 caaggtatag aaaatatatg cagacttgct aaaaatacaa atgttaaaag agtgttttat
     5221 agttctagct ccgaggtgta tggagaatct gtagattttc cacagaatga ggatactact
     5281 ccactaaatg ctcgactacc atatgctgct gtcaaaaatt taggtgaaat ttatttaaag
     5341 acatataata atgaatttgg gttacactat actatatttc gcttttttaa tacttatggt
     5401 cctaaacaaa attctgattt tgtaattagt aaatttatca gacaggcttt caaagaaaag
     5461 caaattacta tttatggtga tggaatgcaa actcgcactt tttgttatat agctgataat
     5521 gtagatgcta cttataatca cctattgcaa tgcgtagaaa atcctaacat gctacctcag
     5581 attattaata taggcaatga caatgaaatt acaatattag aacttgctca aacgataaaa
     5641 aagttgacta attctaatgc tgagataact tttttgccac ctctccaaga aggcgatatg
     5701 aaaagacgac taccagatat tactaagatg aaaaaattac ttaatagacc attgactcca
     5761 ttacatgaag gtattaaaaa tattttagat aataatattt tttattaaat tcaattcaaa
     5821 taaatcaaag actttaatta tatcgaaaga aaattattat ttttgtaaaa ataataatca
     5881 acatagtttt aatgttaaaa aaaaatcagt aaacaaaatt tgtttttatg aataaaaata
     5941 gaaggaaagt acttttaata tacactggag gtactatagg tatggttttt gatgagaaat
     6001 tgaaaacatt agtgcctgtt aactttgata aattaaatag ttatatatcc tatatagatc
     6061 agatgccaat agatatcagg catatttctt ttgagaatcc tattgactct tcaaatattt
     6121 tgcctaagca ctgggtaaaa ctagtagaca taatagaaaa acattatatc gagtatgatg
     6181 gttttttgat tttacatggc actgatacta tggcatatac tgcatcggcg ctaagttttt
     6241 tactcgaaaa cttaaataaa ccagttattt tgacaggttc tcaattgcct ttagatatga
     6301 tcagaactga tggtagagac aatctaatag ctgcattaga aatagcttcc gcatacaaag
     6361 atgatttacc tattgtacca gaagtagcaa tatattttga gaataaatta tttagaggta
     6421 acagaacttc taaatttaat gcagaaaatt tcgatgcttt ttatagtggt aattatccag
     6481 tactagctca agtaggagta catattaaat attatacaga atatattaat aaacctacta
     6541 ataaaccatt aaaagtttac aaaaaattag ataattctgt tggattgatt aagatttatc
     6601 cgggtatgcc aataaaaata ctagaagata ttttatatga taaagatttg aaattagtaa
     6661 ttttagaaac ctttggcact ggcaatgctc ctacttatga tgaattttta aaacctataa
     6721 aaaaagcaat agaagataaa aaagtaatct taaatattac tcaatgcaaa gctggcaaag
     6781 tagagctagg caggtatgct acatctttat ctttagaaaa tttaggagtg atctctggag
     6841 ctgatatgac agtagagtct gccattacca aatcaatgtt tgttttaggc aatttcactg
     6901 ataaaaatca gattattaga ctattacaaa catccttaag aggtgaaatg acagataatg
     6961 aataatgatt tcatctttaa atcatatcga tttcattgat atattatata aataaattct
     7021 aaaattaaat cgctaattga gaatgtgagg atagacgagt agaaaaatta aacctatgtg
     7081 agttgtgtgt tatgatttta tattaaatat ttgattgttt taaattaatt attgtatttt
     7141 tgtatttgaa attttaaaaa taattatttt atggaaaatc aagaattttc agttaaaaat
     7201 gatcaatcta atgtaacaaa atatcaatca ttaccaggag cacaatcatc ctttatttta
     7261 ggtattttgt ccgttgtatt tactttgact acagtgatcg tcggtattat attaggtatt
     7321 atagctgtag tagaagctaa taaagctatg aaagcttatg agataaatcc tagtttgtat
     7381 accaaatctg aatatagcaa tgcaaatgtt ggtaaaatac taggttggat tagtattggt
     7441 ttaggatctg ctttgttttt atttattatt ttgataataa tttttgctgt gagtctggca
     7501 attccgtttt cagcatgtgg ggttatttct agcttttttt aactaataat caatgaaagt
     7561 attgagtata gaagacatta gaaaagctga tgcttatact atggctaatg agcctataac
     7621 atctatagat ttaatggaga gggcagctac taattgtttt gcttttatac ttaaacattt
     7681 ttttgataat gatgattttg taatatttgc tggtcctggt aataatggtg gtgatgggtt
     7741 agtgatagct cgcagattag cttctgtagg atatagagtt aaagttttta tttgtgaatt
     7801 ttctaaatca tattctgaag attgtcttat aaatcttaag aggttaccaa aggatgtaga
     7861 aattataaaa ttaaaaaaca aagatgatat ttttcattta tctaaaataa atgaaaaatc
     7921 aattattata gatgctttat taggttctgg aattactaga cccccagata attggttggc
     7981 cgacttgatt aaaagtataa attcattatc aaatttcaaa atagctatag atatgcctag
     8041 tggtttattt tctgataaat catcaaaggc tcattttgat agagtaatag cagccgatat
     8101 tacattgact tttgaagtac cacgcttagc tttttttatg cccgaaaatt atccttacat
     8161 aggagtatgg acagtgttac caataggatt agattatgat tttattgata aatgcaaccc
     8221 attagctata ataatagatg gtgaattgat aaaacagaaa ttaaaaaaaa gagagccgaa
     8281 agcatataaa aatttatttg gtcatgtatg tatagctgga ggtagtccag gtaaatatgg
     8341 agcagcttta ttatctacta taggcgcttt gaaaagtggt gctggattag tcactagcat
     8401 agtaccaaga gatttgataa gtgtttacct atctcgtgtg ccagaagctc ttactgtatt
     8461 aatagataat ccttttgtca atgatttttc ccttattaat gattatgatg tggcgtgtat
     8521 aggtccaggt ttaggtaatg atccagctac agcacaagca tttcataatt atttaattga
     8581 ttgtaaaaaa ccgacagttt tagatgctga cgcactgaat atcttgagcc agaatattga
     8641 gtggtgtgaa ctactcaatg aaaatttcat tcttacaccc catcctggtg aattaaaacg
     8701 attattagga acttggaggg atgatttcga aaaaatagac aaagcaaaag atttctgtaa
     8761 tacgtataat tgtaatctaa taataaaaga gcataatacc aaaattatta atccacaagg
     8821 tgatttattt ataaatataa ctggtaacga cactttgtct aagggtggta gtggcgatat
     8881 acttgctgga ttgctagctg gtcttttagc tcagaaatat tctccaacag atgctagtat
     8941 catagcagta tgcctacatg gtgctgcagg agagtggtgt ggcaaaagat attcgcatca
     9001 tgccacttct atttctcaat taactgatgg aatagaaaat atttttctta attgggaaca
     9061 aaatgatttt tctatgatca gcattaatac ctttacatat gagggttatc taattattta
     9121 atttgattta atacaaaata attataaaaa tattttaaaa aaacaaactg ataaaaagaa
     9181 tttttagtat atatttgcaa aaaataaaaa aaacaaaact atgactgata aaatagtaaa
     9241 ttcattacga gaatccaaaa ctgcgcgatg gacagcattg attttagtag ctttcacaat
     9301 gttaacagga tattttatag ctgatgtgat gtcgccttta aagccattag tagaggcaga
     9361 attgaaatgg tctagtacag aatatggtat attcactgga gcttatggct ggataaatgt
     9421 attttgttta atgctaataa taggaggaat aattttagat gtggctggta ttagatttac
     9481 ttgtatgctc agtgcaagta tcatgttaat aggtacagct atcaaatatt gggcaattag
     9541 tactccagca ttgaataatt atgtaataaa ttttttgttt tggcctatgc ggggacaggt
     9601 attctgggct tcaataggat ttgctatttt tggagttgga gtagagatgg cggggatcac
     9661 agtgtctaaa acaattgtaa aatggtttaa aggcaaagaa atggcattag ctatgggatt
     9721 agaaatggct accgctcgtt taggttctgc attagctctc atggtatctc ccgtaattgc
     9781 taaatctatg ggtggagtat ctaaacctat tttgtttgga ttgatgttat tgtgtattgg
     9841 ttttattact ttttttatat acatatttat ggataaaagg ttagatgaga gcgaaggtat
     9901 tactaaagaa aacaaaccag aatctgaatt caaatttaaa agcatcggac gtatattgtc
     9961 taataaagct tggtggtata tagctataat gtgtgtttta ttttattctg ccgttttccc
    10021 attcttaaag tatgctagtg atctaatggt aaataaattt aatgtagatc cttctatagc
    10081 aggtgttata ccgggtatct tgccttttgg tactatatta ttgacaccta tttttgggaa
    10141 tatatatgat agaattggaa aaggagttac tataatggta ataggagctt ttttaattac
    10201 tttcgtccac ttaatatttt ctattcctgg tttacatagt gttgttgtcg ctatcatatt
    10261 agttatttta cttggtatag gtttttcgtt agttccttct gctatgtggc cttctgtagc
    10321 taaaatcatt cctgagtctg aacttggtac agcttatgct ctgatattct ggatacaaaa
    10381 ttggggattg atgggcgtgc caatgcttat tggttgggta ttagataaat attgtattac
    10441 aggacaagtg atgagagatg gtgtgttagt caattcttat gattatactt tgccaatgct
    10501 tatttttgct ggattaagtg ctatatctat aatttttgct tttttacttt tagctgaaaa
    10561 taaaaagaaa aaatattctt tgcaactacc taatattgaa gaaaaaataa aagaaaaaga
    10621 agctaatgat taatgattta ttaaaatatt taatattttt ataaatcact ctactaaaat
    10681 aaaagaaaat ttaatgcagt tacctcaatt actgtttcct gttgatgatt taaaaataat
    10741 agaaaaaggt gatatattgt atatatatga tcctattaga tatcaatatt taaagtgcac
    10801 tcctgaagaa tatgttaggc aagctttaat atcacatctg ataaataatt tatcatatcc
    10861 aaaagaatta ctagcagtag agaagcaaat ttttgtaaat aaattatcaa ggcgttttga
    10921 tttagttgtt tttgatagag atatttatcc tttaatttta atagagatta aagctccaaa
    10981 tgtaaaaata aatgataaaa cacttttaca attagcacaa tataattaca ttattaatgc
    11041 aaagcattgg gtattgacta atggtctaaa tgttttattt atgacttatg ataaagaaaa
    11101 taataaaata gattttaaat ctcaaatacc tacatggtat gaattaggta aaagtgataa
    11161 caagtaatag aacaaaataa atgactgaga taacgtcgac tatttgcaaa taagcactat
    11221 ttctttaaaa aaatattata aatatttatc taagtctatt agcagaacgt attttttgga
    11281 tgtctgatct tatgtattta tttgctaaaa ttatcattat aaaagaaata attgggatca
    11341 agacacctat ataatcatac ctatacaaaa tcccatgttt aacccataca tcaggtatta
    11401 gtaatatacc taatagtaag agtacattta agaaaaatgc tacagcattt aagcgtaatt
    11461 gatgtattaa atttttaata cgataaatag tgataggcaa taataatatt agaaggatat
    11521 gaacaactat taatgcatat aaccatccta ttggttgtaa tggagcttta gtatcatata
    11581 tagtaattgg aatattacta ggggatatca atctgataaa aggaataaaa aataataata
    11641 taagcaacaa ccatgctata aataatacaa tattttgctt tctgaataac ataatttttt
    11701 tacaaatata taaaaaataa agaattcaaa atgagaattt aactattgat tatttagcaa
    11761 agatgtgata tttaaaaata ttattcttac attatgttgt taatagactt acctaatgat
    11821 attcatctaa aaagaatcaa agaaaaacaa ataaagtatc gaaattaata attgttatac
    11881 ttacaaaaaa aatttaacat aaaacttatt catttttttg gttatataag gttttatatg
    11941 gattaatgat taaaaaaaag taattctaca agaaattcgt taatattggt ttttttattg
    12001 aacagttaaa tat
//
LOCUS       DAOPRN010000059        11533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_4706_length_11533_cov_106.457832, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000059 DAOPRN010000000
VERSION     DAOPRN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4706_length_11533_cov_106.457832"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PKI65_06730"
     CDS             complement(<1..1076)
                     /locus_tag="PKI65_06730"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOB78170.1"
                     /translation="MKISLLIITFFLTSLSFGQYNEVIYDEENDLHHIQIVQLDNDLL
                     SPSKIISIKTPIVFTSFAFGWKIDQEISPKDILIHYRVHKPKQGWTEWKEDEAYISPN
                     ETRWQMYQTDLLFGLDEGVHDSIEFYYEIPDGVICTGVYLILQDLSPQLNQPIIRDDS
                     EGIILPDRACPALPTMIPRSTWCGSYSACKNPTYTPTYISATHTVIHHGASPDSYTDG
                     AAVVRSYWNYHVNTNGWSDIGYNYLFDKSGNMYVGRYNPSLPNKDVKAAHAGSANSKS
                     IGLNYLGNSDASGTAPTTAQNNKCCQFMAWWYNYKGFDPTSSASILCQDNVTRTKPRI
                     CGHRDVNPGGTTCPGNTLYALLPS"
     gene            complement(1321..1695)
                     /locus_tag="PKI65_06735"
     CDS             complement(1321..1695)
                     /locus_tag="PKI65_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOB78171.1"
                     /translation="MKEDNNIQNIENEKLKFEEITINEATYYTIIPDKYKKRKTFVKK
                     NPNQVFAFIPGVIEKIRVNVKDEINKGDILCDLVAMKMNNKVLAPIDGIIKKIYVKEG
                     DTVPKDHLLFEITPKKVNKKQE"
     gene            complement(1706..3253)
                     /locus_tag="PKI65_06740"
     CDS             complement(1706..3253)
                     /locus_tag="PKI65_06740"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOB78172.1"
                     /translation="MSLRQKTRELKKRMLSAQQGGGMSAIEKQVSMGKLTARERILAL
                     LDPGSFHEYDLFVEHAGKDFDMDKKYLPGDGVVTGTGTIDGHPVCIYAQDFTVAGGSL
                     GYMHAKKITKIMDHALKMKVPLIGINDSGGARIQEGVNSLAGYGEIFYRNTISSGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVDGISKMFITGPEVIKTVLGEEISMEELGGART
                     HASISGNAHFFAYNEFECFTQIKTLLGFIPWNNSQKPEIFEAEEPDTKEYKISKIFPN
                     NEKEPYDVRDIIKAIADASFFFEVQELFAPNIVIGFARIEGMTVGIVANQPLYLAGVL
                     DVDSSDKAARFIRFCDAFNIPIVTFVDLPGYLPGVDQEHSGVIRHGAKILYAYSEATV
                     PKITIILRKAYGGGYIAMGSRHLQADFVFAWPTAEIAVMGPEGAANIIFKKEIQDAED
                     PEEMRKKKVEEYRMKFANPYVAAAYGHIDAVIDPKDTRMYITHSLKVSTNKTVIIPEK
                     KHGIPPF"
     gene            complement(3334..4125)
                     /locus_tag="PKI65_06745"
     CDS             complement(3334..4125)
                     /locus_tag="PKI65_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOB78173.1"
                     /translation="MAINITLISDWGNNNPYLSIFMGELLKNDILHNVIVISNDIAPN
                     DFIDANYLFNATWHHFPNDTIHLITINSNISPDNGFLYINGFNHHFIAPNNGILYYYI
                     HKYFNKLSIYKITNDNIKYQSFYELDYFIFAISNIYQKKSLQQWTKIITDEKEIIPYL
                     FPMPSINNGIVIGEVIHIDNFGNIITNITNDFFQINLMDHKWQMSIRHYKIYKLSKGY
                     IDVNEQNIFAFFNSQDLLELGIKGFNASELLGVKKGEKIYIKIIN"
     gene            4191..5150
                     /locus_tag="PKI65_06750"
     CDS             4191..5150
                     /locus_tag="PKI65_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOB78174.1"
                     /translation="MSEKFFDLHLVDHSGLYGIKDSNIELIRTLFPKLKIIVRGDMLK
                     VIGEDDEIELFYNRFQTLLEHFDKQGKIDENDIMNLMLHDKKELQLNDDLNGKDLILY
                     GRNGVPIKARSPNQKLMLSSINKNDMLFCIGPAGTGKTYTAVAIAVKFLKDKKVKKII
                     LTRPAVEAGENLGFLPGDLKEKLDPYMQPLYDALNDMLPAAKLNSYFEDGTIEIAPLA
                     FMRGRTLENAFVILDEAQNATLGQMKMFLTRMGENSKFIVTGDVTQIDLPPHQTSGLL
                     HTCKILKDIKGINFIFLNETDVMRHPLVSQIIKKFEQNQKNND"
     gene            5249..6370
                     /locus_tag="PKI65_06755"
     CDS             5249..6370
                     /locus_tag="PKI65_06755"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOB78175.1"
                     /translation="MIFSIKESYIYIILIVFTFLSSILLNYLLLNYSKVLRKGKIIKN
                     GERWSDTKTPTIGGTTFFIIFLLSIVFYAQFSLILGLDISIKEVIFLTVGASIAYLLG
                     LYDDTFYLNPILKLLIQLLIAFILIFGDIYIKLFNVNFFNYILTAIWIIGIMNATNLL
                     DNMDGTTSIAILGIAVSILVLCIYNNVIENIFLASLLMIIGIILGFLIFNWPKAKIYM
                     GDSGSMLLGYISAIWGIKYIWNLGNLIPNISYHWVVPFILIAVIYCLPIFDTTITFLK
                     RLIYRRSPLLGGKDHTTHHLIYLGLSNSHVLFLMMLISILNIIVSFNFINHIDHLNSF
                     FYLGIFLYLIIIFAFLVYTSFTNVEKSYPNEKIKRGAIK"
     gene            6339..7319
                     /locus_tag="PKI65_06760"
     CDS             6339..7319
                     /locus_tag="PKI65_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOB78176.1"
                     /translation="MKKLKEGQLNNFLILLLLISTIFLFTFSIKNNNNNKYYEYSQKN
                     YKVFTFAIPDTVYFAGQLIDLTNPFIRERVESEIYSIAYFHSQFIKTVKQTERYMPYI
                     EQELKNKAMPSDLKYIAIIESNLMPTTSPAGAKGIWQFMKGTATKYGLEVTDDVDERL
                     NFEKSTIAALSYLHDLYNMFGDWLLTIAAYNAGENYIKNKITQQYTNNFWDLVMNAET
                     ARYISRAIATKLIYQSLKTYGFYIPVLELYPPYKYKEIAIDTSIKDLPLFAKQMNVSY
                     YNFKKLNPWLISDKLSNAGNKKYIIRIPVDTVFKITNKLNNDTIKLIERI"
     gene            complement(7412..8374)
                     /locus_tag="PKI65_06765"
     CDS             complement(7412..8374)
                     /locus_tag="PKI65_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOB78177.1"
                     /translation="MDGQRMLRMVKKVTNFFQLIRWIDLLLISLIFYLVYNQILVNFY
                     AIDGTAMPINGLEYFLISLAFILMAAGGYALNDYYDYGMDEINKPNRLILHHKLPYKT
                     GLYSFIILTSLGIILSFYQCLVWKNGELFIIYIFVAAMFWFYSSKFKRELIIGNVIVS
                     LLAAFNILLPWLIVIFNGIDAHLLPISMWKTMNFFIFTYALLSFFITFAREVAKDIVD
                     IDGDKEYNCQNIPIKYGINTAKWVVLASLIFIMVELIYVTIRLFNSAYIIMGYYFAFI
                     LIPFGIYVMILTLKAKEKSQWKNISDIMKIYIIAGILSMFFIKL"
     gene            complement(8344..8865)
                     /locus_tag="PKI65_06770"
     CDS             complement(8344..8865)
                     /locus_tag="PKI65_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOB78178.1"
                     /translation="MKNYKEKLKNITTIILDYDGVLTDGKVYIVENGREIRAGNVKDS
                     YILQLLIKKGFNVIMISGSNADSIRWRCKSLNIENIHLGIVNKLTEYELIKKKFNIND
                     YEILFIGDDIPDYELMINAGVSVCPADAAQEIKEVADYISRYEGGKGCVREIGEQILK
                     AQNKWMDKECLEW"
     gene            complement(8852..9598)
                     /locus_tag="PKI65_06775"
     CDS             complement(8852..9598)
                     /locus_tag="PKI65_06775"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOB78179.1"
                     /translation="MKIVLIGYGNVGWHLSRFLKPVCEELMVVTSKPNDKDDFFFSDT
                     IPENADFYIITRQEKFVSDTSKKIKTNNGIVVHTSGTLPAEVLNKHRHYGVLYPFQTF
                     RKGRPLSVPFINFAIEANDQFTINQLTNLCDSIHQNYYLVTQNQRIALHVTGVLSSNF
                     PNFLYVISYDILEKYNLHYEILKPLIKETIERLDYFHPADVQTGPAIRNDSEIIAKHL
                     ELLKKDLPIGADIYEVISKAIIKYYLNEKL"
     gene            complement(9595..10044)
                     /gene="smpB"
                     /locus_tag="PKI65_06780"
     CDS             complement(9595..10044)
                     /gene="smpB"
                     /locus_tag="PKI65_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOB78180.1"
                     /translation="MANKVEIVNKKAKFEYTILDRYEAGIVLTGTEIKSIRQNKASIS
                     DAYCVFKDGELWVKNMHIAEYSHGTYNNHEPKRDRKLLLHKHQLRRLESKVKESGLTI
                     VPLRLYINEKGIAKLEIALVRGKKEHDKREAIKKRETERNISKFFKL"
     gene            complement(10179..10880)
                     /locus_tag="PKI65_06785"
     CDS             complement(10179..10880)
                     /locus_tag="PKI65_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOB78181.1"
                     /translation="MKNNMIDTFDICKDCLFKSICLQSMSKEDFDLLYESTIQKSYSK
                     GDIIFKQDDKSLNLVFLQSGIVKFNFTDENNKNFILTIVSAPSLLGLANILNEDINIF
                     SIVAIEDSMGCQLDLNKLKILAMNNKNFMINLSKLSTDMFRNSIYNVISLAHKQINGR
                     IADILLFLARNVYHSNTFTLSISRQELAEYAGCSKENVIRSLRSLNRDNIIRVSGKEI
                     EILDLKKLIKISKKG"
     gene            11131..>11533
                     /locus_tag="PKI65_06790"
     CDS             11131..>11533
                     /locus_tag="PKI65_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78182.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLFSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILA"
BASE COUNT         4058 a         1720 c         1540 g         4215 t
ORIGIN      
        1 aatgaaggta ataaagcata taatgtattc ccagggcaag tagtaccacc aggattcaca
       61 tctctatgtc cacaaatacg tggtttagtt ctagtaacat tatcttgaca taatatacta
      121 gcagagctag taggatcaaa acctttgtag ttataccacc aagccataaa ttgacagcat
      181 ttattgtttt gtgctgtcgt aggagctgta ccagaagcat ctgaattgcc taaataattt
      241 aatcctattg atttagaatt tgcactgcca gcgtgcgccg cctttacatc tttattagga
      301 agactcggat tatatcttcc cacatacata tttccagatt tatcaaataa atagttatat
      361 cctatatctg accatccatt tgtatttaca tgataattcc aataactacg cactactgct
      421 gcgccatctg tgtatgaatc tggtgaagct ccatgatgta ttactgtatg agtagctgat
      481 atgtatgtcg gggtatatgt gggattttta cacgcagagt atgacccaca ccacgtagag
      541 cgtggtatca ttgttggtaa tgcagggcaa gccctatcag gcaatattat accttcgcta
      601 tcatctctta ttattggttg atttaattgt ggtgataaat cctgtaaaat taaatatacc
      661 ccagtacata ttactccatc aggaatttca taataaaatt cgatactatc atgcactccc
      721 tcatctaaac caaataacaa atctgtttga tacatctgcc atcgagtttc attgggagaa
      781 atgtatgcct catcttcttt ccactctgtc caaccttgtt ttggtttgtg taccctgtaa
      841 tgaattaaaa tatccttcgg tgatatttcc tgatctattt tccatccaaa agcaaaggat
      901 gtaaaaacta ttggtgtctt gatactaatg atcttacttg gcgataatag atcattatct
      961 aattgtacaa tttgtatatg atggagatca ttttcctcat catatattac ttcattatat
     1021 tgaccaaaac tcaatgaggt caaaaaaaat gtaataatca ataatgatat tttcatgtta
     1081 attattttac aaattaaata cgaaattaca taaatttata taaaaagtct aactaatatt
     1141 atatttttat ataatattag ttagacaatt atgtttagta ttaaatttct tttattattc
     1201 tgatcacacc atctaaatga taagatagaa ttggctaaag cattttgaag ttttatatat
     1261 taataaaaaa acttctttat tataaaaatt ataaaaaagt tatgaaacca tcaaaaattt
     1321 ttattcttgt tttttattaa cttttttagg agtgatttca aagagcaaat gatcctttgg
     1381 tacagtatca ccttctttaa cataaatttt tttaattatt ccatcaatag gggccagaac
     1441 tttattattc attttcatag ccactaaatc gcacaagata tcacctttat taatttcatc
     1501 ttttacattt actcttattt tttctattac tcctggaata aatgcaaaga cttgattagg
     1561 atttttttta acaaaagttt ttcttttttt gtatttatca ggtataatag tataataagt
     1621 ggcttcatta atggtgatct cttcgaattt taatttttcg ttttctatat tttgaatatt
     1681 attatcttct ttcataataa atgttttaga atggagggat gccatgtttt ttttctggta
     1741 taataaccgt tttatttgta gatactttta atgaatgagt tatgtacatt cgagtatctt
     1801 taggatctat tacagcatct atatgaccat atgctgcggc tacataagga ttagcaaatt
     1861 tcattctata ttcttctact ttcttttttc tcatttcttc agggtcttcg gcatcttgaa
     1921 tttctttttt aaaaataata ttagcagccc cctctggtcc cataacggca atttcagcag
     1981 taggccaagc aaaaacgaaa tcagcttgca aatgtctcga acccatagct atataaccgc
     2041 caccatatgc tttacggaga attatagtga ttttaggaac ggtagcctca ctgtaagcat
     2101 ataaaatctt agcaccgtgt ctgattactc cactatgctc ttggtctact ccaggcaaat
     2161 atcctggtaa atcaacaaat gtaactatag gtatattaaa agcatcgcaa aagcgaataa
     2221 accttgcggc tttgtcgcta ctatctacat ctagcacgcc agctaaatac aatggttgat
     2281 tagctactat acctactgtc ataccttcta ttctagcaaa gcctattact atattaggtg
     2341 caaaaagttc ttgtacttca aaaaagaaac tagcatctgc tatagccttt ataatatctc
     2401 taacatcata aggttctttt tcgttattag ggaaaatttt tgagattttg tattcttttg
     2461 tatcaggttc ttctgcttcg aatatttcag gtttttgtga attattccat ggaataaagc
     2521 caagtaaggt tttaatctgt gtgaaacatt caaattcatt ataagcaaag aaatgtgcat
     2581 taccactaat agaggcatga gtacgagcac cacctagttc ttccattgat atttcttcac
     2641 ctaaaacggt tttaatgact tctgggccag taataaacat tttagaaata ccatctacaa
     2701 caaaaacaaa gtctgtaaga gcaggagaat atactgctcc gccagcacaa ggtcctaaaa
     2761 taacactaat ctgaggtatc acgccagatg aaatcgtatt tctataaaaa atttcgccat
     2821 aaccagcaag gctattaacg ccttcttgga tacgcgcacc accagagtca ttaatcccaa
     2881 tcaatggaac tttcatcttg agggcatgat ccataatttt tgtgattttt tttgcatgca
     2941 tatatcccaa agatccacct gcaacagtaa aatcctgagc ataaatacat actggatgtc
     3001 catcaatagt gccagtgcca gttactacgc cgtcaccagg caaatatttc ttgtccatat
     3061 cgaaatcttt acctgcatgc tctacgaata aatcatattc gtgaaaagaa ccaggatcta
     3121 ataaggctaa tatacgttca cgagctgtga gtttacccat agatacttgt ttttcgatgg
     3181 cactcatacc gccaccttgc tgagcactta acatccgttt ttttaattct cttgtttttt
     3241 gtcttaatga cataatttta atttttttaa aattaattga actgcaaaga taactttatt
     3301 tttttaaatg tgattttttt aattgttttt gcattaatta atgattttga tataaatttt
     3361 ttcacctttt ttcacaccta atagctcact tgcattgaaa cctttaattc ccaattctaa
     3421 taaatcttga ctattaaaaa aagcaaaaat attttgttca tttacatcta tgtaaccctt
     3481 tgacagctta tatattttat aatgtctaat tgacatttgc catttatgat ccatcaaatt
     3541 gatttgaaaa aaatcattag ttatattagt aataatatta ccaaaattat ctatatgtat
     3601 tacctcgcct ataactatgc cattattgat agagggcata ggaaataaat atggtattat
     3661 ttctttttca tcagttatta ttttagtcca ttgctgtaga gatttttttt gataaatatt
     3721 agaaattgca aatataaaat aatctaattc ataaaacgac tgatatttta tattatcatt
     3781 agtgatttta tagatagaca acttattgaa atatttatga atataataat ataaaatacc
     3841 attattagga gctataaaat gatggttaaa tccattaata tataaaaaac cattatctgg
     3901 agatatatta gaattaatag taatcaaatg tattgtatca tttggaaaat gatgccaagt
     3961 agcattaaat agataattag catcaataaa atcattagga gctatatcat tagagataac
     4021 aataacatta tgcaaaatat cgttttttaa taattcaccc ataaatatac ttaaatatgg
     4081 gttattattc ccccaatcac taattaaagt tatattaata gccattgcag ttaactaatt
     4141 ttatttgtaa ttttgcatta ttaaattcaa aaataaaaat atttatttta atgagtgaaa
     4201 aattttttga tttgcatcta gtagatcata gtggattata tggaataaaa gattcaaaca
     4261 ttgaactgat aaggaccctt tttccaaaac taaaaataat agtaagagga gacatgctaa
     4321 aagtaatagg agaagatgat gaaatagaac ttttttataa tagatttcag acattactag
     4381 agcattttga taaacagggc aaaatagatg aaaatgatat tatgaatttg atgcttcacg
     4441 ataaaaaaga attgcaatta aatgatgacc taaatggaaa agacttaatt ctgtatggac
     4501 gtaatggggt acctataaaa gctcgtagtc ctaaccaaaa actaatgtta agctctatca
     4561 ataaaaatga tatgttattt tgtataggtc cggctggtac aggtaaaaca tatactgctg
     4621 tagctatagc ggtgaaattt ttaaaggata aaaaagtaaa aaaaatcatc ttaactcgtc
     4681 cagctgtgga agcaggtgaa aacttaggat ttcttccagg agatttgaaa gaaaaattag
     4741 atccatacat gcaaccatta tatgatgccc ttaatgatat gttacctgct gctaagttaa
     4801 attcatattt cgaagatggc actatagaaa tagcaccatt agcttttatg cgtggtagaa
     4861 cattagaaaa tgcatttgtc atcttagatg aggctcagaa tgctacacta ggacagatga
     4921 agatgttttt aactcgtatg ggagaaaatt ctaaatttat cgttactgga gacgtaacac
     4981 aaatagacct cccaccacat caaacttcgg gcttgttaca cacttgtaaa atattaaaag
     5041 atatcaaagg tatcaatttt atatttctaa atgaaacaga tgtaatgcga cacccattag
     5101 tatctcagat tataaaaaaa tttgagcaaa atcaaaaaaa taacgattaa atattatttt
     5161 caatctatta ttaattattt ttattaaaaa aattttttta agtaaaattt actaattttg
     5221 ttattttaaa ttttattata taaaatttat gatttttagt atcaaggagt cgtatatata
     5281 cataatttta attgttttta cttttttatc aagtatatta ttaaactatc ttttgctcaa
     5341 ttatagcaaa gtattaagaa aaggcaaaat aatcaaaaat ggtgaaagat ggtcagatac
     5401 taaaacacct actatcggag ggactacttt ttttatcatt tttttgctat ctatagtatt
     5461 ttatgctcag ttttctctta tattaggttt agatattagt ataaaagaag tgattttttt
     5521 aactgtgggt gctagtatag cttatttact aggtctttat gatgatacat tttatttgaa
     5581 tcctatctta aagttattaa ttcaattatt aatagctttt atattaatat ttggcgatat
     5641 atatataaaa ttatttaatg taaatttttt taattatatc ctcacagcaa tatggataat
     5701 aggcataatg aatgctacta atctgctaga taatatggat ggtactacta gtatagctat
     5761 attggggata gcagtttcga ttttagtttt atgtatatac aataatgtaa ttgaaaatat
     5821 atttttagct tcattattaa tgattatagg tataatatta ggttttttga tatttaattg
     5881 gccaaaagct aaaatatata tgggagatag cggcagtatg ttactgggtt atatatcagc
     5941 tatatggggc atcaagtata tatggaactt aggtaatttg atccctaata tttcttacca
     6001 ttgggtagtt ccatttattt taatagcagt tatctattgt ttgcctattt tcgatactac
     6061 tataacattt ttgaagagat tgatttatcg tcgttcacct ttattaggtg gcaaagatca
     6121 cactacacat catctgattt acctaggatt atctaattca catgtacttt ttttgatgat
     6181 gctcatatct attttaaaca taatagtatc ttttaatttt attaatcata ttgatcatct
     6241 gaatagtttt ttttatttag gtattttttt atatttaata attatatttg catttttagt
     6301 gtatacatca tttacaaatg ttgaaaaatc atatccaaat gaaaaaatta aaagaggggc
     6361 aattaaataa ttttttaatt ttattattat taataagtac aattttttta tttacttttt
     6421 caataaaaaa taataataac aataaatatt acgaatattc acaaaaaaat tataaagttt
     6481 ttacttttgc tattccagat acagtttact ttgctggaca attaatagac cttaccaatc
     6541 cctttatacg agaacgtgta gaatctgaga tatatagtat agcttatttt cattctcaat
     6601 ttataaaaac agtgaagcaa acagagaggt atatgcccta tatcgaacaa gaattaaaaa
     6661 ataaagcaat gccatcagat ttaaagtata tagctatcat agagagcaat cttatgccta
     6721 ctacatcccc agctggtgct aagggaattt ggcaatttat gaaaggtact gccactaaat
     6781 atggattaga agtaacagat gatgtagatg agagattaaa tttcgaaaaa tctactattg
     6841 cagctttatc ctatttacat gatttatata atatgtttgg tgattggctt ttaactatag
     6901 ctgcttataa tgctggtgaa aattatataa aaaacaaaat aactcaacaa tatacaaata
     6961 atttttggga tttagtgatg aatgccgaaa ctgcacgata tatttctaga gctatagcta
     7021 ctaagctaat atatcaatca ttgaaaactt atggttttta tataccagta ttagagcttt
     7081 atcctccata taaatataaa gaaatagcaa tagatacttc tataaaagac ttaccgcttt
     7141 ttgctaaaca gatgaatgtg tcttattata atttcaaaaa attaaaccct tggctgataa
     7201 gcgataagct ctctaatgcg gggaataaaa aatatattat tcgcattcct gtggatactg
     7261 tttttaagat aacaaataaa ttaaataatg acacaataaa attaatagaa aggatttaat
     7321 aaacttaaaa tttaaagttt ttttttaacg ttaatataga taaattttaa atattttgct
     7381 tttataatta aaaatacatc aaaaatattt cttataattt tataaaaaac atagataaaa
     7441 taccagctat aatatatatt ttcattatat cactgatatt tttccattga gatttttcct
     7501 ttgctttgag agtaagtatc atgacataaa taccaaaagg gattaatatg aacgcaaagt
     7561 aataccccat tataatatat gcactattga atagtctaat agttacgtat attaattcta
     7621 ccataataaa aatcaaggag gctaatacaa cccatttagc agtattaata ccatatttta
     7681 taggaatgtt ttgacagtta tattctttgt ctccatctat atctacaatg tcttttgcga
     7741 cttctctggc aaaagttata aagaatgata aaagagcata cgtgaaaata aaaaaattca
     7801 tagttttcca catacttatt ggtaatagat gagcatcaat accattaaat attactatta
     7861 accatggcaa taaaatgtta aaggctgcca atagacttac tattacatta ccaatgataa
     7921 gttctcgttt aaatttagaa ctataaaacc aaaacatagc tgctacgaaa atatagataa
     7981 taaatagttc accatttttc cataccaaac attggtaaaa actaagaatt ataccgagag
     8041 aagtaaggat tataaatgaa tacaatcctg ttttatatgg cagcttatga tgaagtatca
     8101 gcctattggg tttattgatt tcatccatac cataatcata ataatcattt agagcatatc
     8161 cacctgcagc catcaagata aaagcgagtg aaatcaagaa gtattctaat ccattaatag
     8221 gcatagcagt accatcaata gcataaaaat ttactaaaat ttggttataa accaaataaa
     8281 agattaaact aattaataat aggtctatcc acctgatcaa ttgaaaaaag ttagtaactt
     8341 tttttaccat tctaagcatt ctttgtccat ccatttattt tgcgctttta agatttgttc
     8401 acctatttct cttacacaac ctttaccgcc ttcgtatctt gaaatataat cagctacttc
     8461 tttgatctct tgagctgcgt cagcagggca tacagatact cctgcattta tcataagctc
     8521 ataatctggt atatcatcac ctataaaaag gatttcatag tcattgatat taaatttttt
     8581 ctttattaat tcatattcag tcaatttatt tacaatgcct aaatgaatat tttctatgtt
     8641 tagcgattta catcgccatc gaatagaatc agcattacta ccgctaatca taatcacatt
     8701 aaatcctttt tttattaata attgcaagat ataactatct tttacgttac cagcccttat
     8761 ttccctacca ttttctacta tatacacctt gccatctgtc aatacgccat cataatctaa
     8821 aattatagtt gttatatttt ttaatttttc tttataattt ttcatttaaa taatatttta
     8881 ttattgcttt gcttattact tcatatatat ctgcacctat tggcaaatct ttttttaata
     8941 attctaaatg tttagctatg atttcactat catttcttat agctggtcca gtctgtacat
     9001 ctgcaggatg aaaataatct aacctttcga tagtttcctt tattaatggt tttaaaattt
     9061 cataatgtaa attatatttt tctaagatat cataagaaat tacataaaga aaatttggaa
     9121 aattggaaga taaaactcct gtaacatgaa gagctatacg ttgattttga gtgactaaat
     9181 aataattttg atgaatcgaa tcacataaat ttgttaattg attaatagta aattgatcat
     9241 tggcttcgat agcaaaatta ataaaaggca ctgacaatgg tctgcctttt ctaaatgttt
     9301 gaaaaggata taaaacccca taatgcctat gtttattcaa tacctctgca ggcaatgtgc
     9361 cagaagtgtg tactactatg ccattattag ttttaatttt tttggacgta tcactaacaa
     9421 atttttcttg acgtgtgatg atataaaaat cagcattctc tggtatagta tcagaaaaaa
     9481 agaaatcatc tttatcattt ggttttgaag ttacaaccat caactcttca caaacaggtt
     9541 ttaaaaatct actaagatgc catcctacat taccataacc tattaagact attttcataa
     9601 tttgaaaaat ttagaaatat tacgttcagt ttctcttttt tttatagctt cccttttatc
     9661 atgttctttt ttacctctta ctaaagctat ttctaacttt gcaatccctt tttcattgat
     9721 ataaagacgc agaggcacaa tagtaagccc agattctttt acttttgatt ctaatcttct
     9781 caattgatgt ttatgtaata gcaattttct atctctttta ggttcatgat tattatatgt
     9841 tccatgtgaa tactcagcta tatgcatatt tttgacccat agctcaccat ctttaaatac
     9901 acaataagcg tcagatatag aagctttatt ttgtctgata ctttttattt ctgtaccagt
     9961 gagtactatt ccagcttcat atctatctaa aatagtatat tcaaatttag cttttttatt
    10021 tactatttct actttatttg ccatatctca tttaagaata caaagataat tgttttttaa
    10081 taatattatg aataattata aacattactg aaatttattt agctataaat caagtttgag
    10141 tcttgttata gagtaatatt acatattaat tttttgtttt atcctttttt actaattttt
    10201 attaattttt tcaaatctaa aatttcaatt tctttcccgc taacacgaat tatattatct
    10261 ctattaagag atctcaaaga acgaatgaca ttttcttttg agcagccagc atattcagcc
    10321 aattcttgtc tagaaataga taaagtaaaa gtattgctat gataaacatt tctagctaaa
    10381 aacaataaaa tatcagctat gcgaccatta atttgtttat gagccaaact aataacatta
    10441 taaatagaat ttctaaacat atcggtagat aatttagaca agttgatcat aaaattttta
    10501 ttattcatag caagtatttt caatttatta agatctaatt ggcaacccat cgaatcttct
    10561 atagctacta tagaaaagat attaatatcc tcattcaata tatttgctaa tcctagtagt
    10621 gagggagcac ttactattgt taagataaaa tttttattat tttcatcagt gaaattaaat
    10681 tttactatac cactttgcaa gaaaactaaa tttaaagatt tatcatcttg tttaaaaata
    10741 atatcgcctt tagaataaga tttttgtata gtactttcat ataagagatc aaaatcttct
    10801 ttgctcatcg attgcaaaca aatagattta aaaaggcaat ctttgcatat atcaaaagtg
    10861 tctatcatat tatttttcat gttaatttta taatattatt tacaaaaata caagaaataa
    10921 atgattcaaa attgatatta taaattttgt aagcgtaaaa taaaaatctt gtaagtatga
    10981 ttttaatgtg agagataatg tatagttgta atgtataaaa aatattttaa ggtgagcaaa
    11041 atgggaggga atttatgaaa ttaataaatg tttaagtttt tcaagtttat atgttaaata
    11101 atttttttat ttttgcaaaa attttaattc atgaaaaaaa tcgtagagaa taccagtaaa
    11161 tatcttgcta tgagtaagga agagaataaa gaattcttag acaacctttt ttctctaatg
    11221 aaaaaattag aagccgatgt agtcgattat caaggtctga aaatactcag tgccccacca
    11281 ctatgcccag actgtcgtag caatcacatc attaaaaatg gcttacaaaa tgggatgcaa
    11341 aactatcgct gcaaagactg cggcagacag tttagagtta ccactggaac ttttgtttac
    11401 aggttgcaaa agaaagaaaa aatgctcgac tacatcagat gtatggtagc aggcaaaagt
    11461 ctcagagcat gcgctaaaga agtgggcatc agcctaccta caagcttcgc ttggcgacac
    11521 aagatattag cag
//
LOCUS       DAOPRN010000060        11453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_4752_length_11453_cov_151.785752, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000060 DAOPRN010000000
VERSION     DAOPRN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4752_length_11453_cov_151.785752"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..965)
                     /locus_tag="PKI65_06795"
     CDS             complement(198..965)
                     /locus_tag="PKI65_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOB78183.1"
                     /translation="MNRTIVITGASSGIGAATAKKFAINGDNLILLARRIDKLESIKK
                     EISTLSNVKVLIDKVDVRNKKEVFNFFSNLTIDWQNIDILVNNAGLALGLSPLHEGNI
                     EDWEVMVHTNIMGLLYVSRAVLPGMVKRCKGHVINLGSIAGREVYANGNVYCATKHAV
                     HALSEAMRIDLLPFNIKVTNVAPGAVNTEFSTVRFKGDRDLADQVYTGYTPLMANDVA
                     DAILYAANLPDHVNVNDILVMPTHQANATIFNKKNNT"
     gene            complement(962..2272)
                     /locus_tag="PKI65_06800"
     CDS             complement(962..2272)
                     /locus_tag="PKI65_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78184.1"
                     /translation="MEIVIAIIIAIFLTIFIFFLTKRYSNEIAPVWWMIAFILKILAG
                     IFLIFIYTNYYFDRNKADIFKYYDDGKILWNSSKDNPNDFIKIIFGIYDKNEYQTLKD
                     KYLINTSNWERANYVPITCDNRMMIRINAILMPLTGGNYLLHMLFFVFLAFIGQFLFF
                     KSINPYIKNAKLSFIIIFFFPSLVLWTSGVLKEALVMFLLGLWFFSIHKIITKPVSIF
                     TIILFILVTILFFTVKIYIIIGLFLAIFPLIISKVLKKIEIWKLYLVFLLFYILCIPL
                     IKWIFNLDLLMIVVNKLTLATNLAISENAGSLINPLNLKPTIFSFILNAPLALVNTLF
                     RPFVTDLHNIIVIPAFIENVLWISFAIFTFIFHKKSLHRNNKLILFSFLLFALIEFLL
                     IGYTTPIVGAIVRYRIFPLLFLLLSFVIIIDIEKICKKFKIKHL"
     gene            complement(2314..3048)
                     /locus_tag="PKI65_06805"
     CDS             complement(2314..3048)
                     /locus_tag="PKI65_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOB78185.1"
                     /translation="MSIQIYNLFLKTRTIAILIDPDKTDKELLDKYIYAGNNDCCDLY
                     LIGGSLIFDIEWFRNIIITLKSKTSLPVIIFPGNYTQIISDADGILFLQLFSGRNPEY
                     LISQQIIAAPIIYKSGLPAISTAYILIDGGNISSTAYMTQSLPIPPDKKDILIATCLA
                     AKYAGAQLIYLEAGSGACNPIKTEILTNLKNIISLPIIIGGGINNLNIIDQYFNHDIN
                     GVVIGNHIEKDNNFLEELKEFRKNIY"
     gene            3080..3595
                     /locus_tag="PKI65_06810"
     CDS             3080..3595
                     /locus_tag="PKI65_06810"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOB78186.1"
                     /translation="MRKIFLLCMFFCPLQLLNSQNTYIVTYDTILKQEILIGRLNKND
                     LMNSDFAEYFYLYHEAYKAEVGIINKLKHLINDVNITIVMGTWCSDSQEQIPKFYKII
                     NLLEYDENKILLIGVDKKKQAREYESLVQSLNIEKIPTFIFYKDEKEIGRIIESPETT
                     LEMDMLKILNK"
     gene            complement(3605..4108)
                     /locus_tag="PKI65_06815"
     CDS             complement(3605..4108)
                     /locus_tag="PKI65_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78187.1"
                     /translation="MKSSKILILFIIIANFLFSNIENVSAQLPEIKEITFVLKTPPLS
                     PEYYREYEFIIKKNKIQLKIKRQNELLDKTKVKITADAWNEIIKYFNANKIQIGPEKT
                     KYDYEGCVGGVTYLVTIKFKNKEIKKGLTYECGNKQFGNMEGQVKKLHDYLKSLIPNF
                     RELYELN"
     gene            complement(4105..6147)
                     /gene="ligA"
                     /locus_tag="PKI65_06820"
     CDS             complement(4105..6147)
                     /gene="ligA"
                     /locus_tag="PKI65_06820"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOB78188.1"
                     /translation="MTKEEAIDRIKYLTAELNRHNYLYYVKNSPEISDYEYDQLLKEL
                     ERLEKQYNYQEPDSPTQRVGGFITKEFPNQPHRFPMLSLDNSYDWNEVVSFFKRNITN
                     VDDVEFVAELKYDGLAISCIYENGILITALTRGDGTIGEVVTNNVKTIKNVPLRLFGD
                     YPNWVDIRGEIIMPFDSFNKLNKEREENSEYPFANPRNAAAGSLKLQDSSLVAKRNLQ
                     FMPYEVLTETPIFSNHYEAMMKAKEWGAPVPPYIKLCHGLNEVKIFIDEYEIKRDELP
                     FGIDGVVIKINNYELQRQLGNTAKYPRWAVAFKYKPEQALTQITSVDFQVGRTGVITP
                     VANLNPVLLSGTTVKRVTLHNFDFVKQNDIHVKDYVWVEKSGEIIPKIVKVEVDKRPP
                     DAYPVCPPRFCPACNSPTLHKEGDPFVYCTNDKFCPPQILGKLQHFISKDAMDIETLG
                     EEKIELLYRKGLVKSPADLYNLTYDQLLGLENQYQMPDGKIRIVRFQHKTVENILKSI
                     EKSKSKPFDRMLYALGIKNVGSVMAKNIANYAKSLDKLMQMSKEELQQIPEVGDITAQ
                     SIIDWFSDPDNLLLIERLKKAGLKFALDEETPKSEKLKGLNFVISGVFEDIERDDLKR
                     LIEANGGKVLSSVSSNTDYLIAGDNMGPAKRQKANELGIPIISFKEFVEKFKINNI"
     gene            complement(6382..7641)
                     /locus_tag="PKI65_06825"
     CDS             complement(6382..7641)
                     /locus_tag="PKI65_06825"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOB78189.1"
                     /translation="MRPNIITPTKPKIPEVNEINLSDNNKIYTFHTTRTDLVSLYLII
                     PLGYKFANNPVIGKFAWEMIKSGTKQKTAIQIAEEINYYGASIDISVGPDSVNISLIA
                     LNNLFIHALNLLFELLTDPTYPKKELILQKQETLGILKIKNKRVDFIANKNFFKLLFG
                     NNHPYGKHIKYSDILLTSRKNLITFQKKYFDFSNSLWGLAGNYNDEIIRFITEHYDIL
                     PHLNIKDLKLSAGMPSQKQLHINKKDSLQTAIKIGCQIKSLKEKDYPYFLIGQTIFGG
                     YFGSRLNKNIREDKGLTYGIYCRTNHFKHQAAWSISAEVKAEQTNFVIDEIKKEYDKF
                     VNDTISNEELDRVRNYLMGTSLFAFDGAFNIIKNKLKMIDLGTSMKTYYENLFYVINE
                     AKENEIREKFVEYVPWNKMIMVTVGKK"
     gene            complement(7647..8885)
                     /locus_tag="PKI65_06830"
     CDS             complement(7647..8885)
                     /locus_tag="PKI65_06830"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOB78190.1"
                     /translation="MLNKTFHKGKLSNGFRYILYPVEDTTLVCVNVFYFVGSRDEEPD
                     KTGFAHLFEHLMFAGSEYAPSYDSEVESAGGINNAFTTQDFTDYFIQLPSYNIELALW
                     LESDRMQHLNINEKSLNIQKNVVIEEFKQTHLNQPYGDLLHILFDMCYARHPYRWPTI
                     GLTPEHIEKATLEDVKRFYDKWYQPSNAILAISGKYHDDIEMLIEKYFSDIPSTPIDE
                     RDYPLESPIKHQVRKVVKRNVPLNLIVIAIPMPGRLSPQYSTYDILSDILGNGNSSRL
                     YTSLVKEKKLFISIDASVMGSVDPGLLLILGFVKPSIDVKKAEDTLWNELVNFRKKPV
                     TDKELDKVRNMYLSTLSMNIYDLQRIGFELSFYEALGSADLLFENYEIYKSITKDEIM
                     NLADDILKPDNAFVLNYLKK"
     gene            complement(8982..9170)
                     /locus_tag="PKI65_06835"
     CDS             complement(8982..9170)
                     /locus_tag="PKI65_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78191.1"
                     /translation="MGRAGQRSFFNYPVRLCLTPLRRRGITSYSPLVEGMSHRDRGVK
                     TSGDLSFVSFLCVAGKEK"
     gene            complement(9342..9875)
                     /locus_tag="PKI65_06840"
     CDS             complement(9342..9875)
                     /locus_tag="PKI65_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78192.1"
                     /translation="MKIIKSLIFIIFAIFLLHIILLLYNSCEQHKREKEFLDKYEYED
                     FSKFKGINMEIRSYDDSGKILIPGRIDFLKNDSLKFGYYTLKMDTQDNNITYMHWICA
                     DKEVELDTIKLQHLAKNFMKYQISRLDVDTAGNVFVYIKDFANRAFVRFANEDELQKR
                     NRESTWTKVKNTDNWYK"
     gene            complement(9881..10870)
                     /locus_tag="PKI65_06845"
     CDS             complement(9881..10870)
                     /locus_tag="PKI65_06845"
                     /inference="COORDINATES: protein motif:HMM:NF026945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOB78193.1"
                     /translation="MKIHYHSDHLGSASFVTNAEGAAVQHLQYLPYGELFVSQRNTEE
                     FDSRYKFTAKELDNETSYTYFEARYYDSELSGWLSVDPMSDKYPSLSPYCYTADNPVV
                     LVDPNGTSISEFDENGNYLGTKKDNWFHNTFFGRTGRIVDSDGNVTQKFKFADPKNDV
                     NDLINGKINKIHFVQEEEIISMLSRAGAFNQENKTASNDLFNRYDYIGEEGKGGGKFD
                     FATTEEWGLKYQYPNITKEQTLFLVDGVAHNDMNFGNFLFGAAGKAIGFTLPELLMGA
                     HYNSLFNSNTNGYKPQIDSRDDQLSIYMGVRHANKYKYKDMYYRVEVHPLIKN"
     gene            complement(11170..>11453)
                     /locus_tag="PKI65_06850"
     CDS             complement(11170..>11453)
                     /locus_tag="PKI65_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78194.1"
                     /translation="LGLDPYGFFYYKNHMFIVDGDSLEELFSICGQKVSFKYLEKDLD
                     YIIEQNTKRKYDKNKILELDFIIDDSFSQWSYIYINGEFIFEGKSSFCN"
BASE COUNT         3908 a         1808 c         1512 g         4225 t
ORIGIN      
        1 ctagaaatct acgtttctgc tctttgggta gagcttgata atctaataga ttgaaattat
       61 ccataattat ttttttgtaa aattaaataa aaattatttg ataatgatat aaactacttc
      121 aagtaaaaca aagattaatt tcagaaagtc tcttccaaca aatcaccctt taggaagctc
      181 aatagcttca aactttatta tgtgttgttc tttttgttaa atatggtagc atttgcttga
      241 tgagtgggca ttaccagtat atcatttaca ttgacatgat caggtaggtt ggctgcgtat
      301 aatatagcat cagctacatc atttgccatc agaggcgtat atcctgtata tacttgatct
      361 gcaagatctc tatccccttt gaaacgtacg gttgaaaatt cagtgtttac agcaccagga
      421 gcaacatttg taactttaat attaaatggt aataaatcta ttctcatagc ttctgacaat
      481 gcatgtacag cgtgcttggt agcacagtaa acattaccat tagcatatac ttctcttccg
      541 gctatagaac cgagatttat cacatgtcct ttgcatcttt ttaccattcc aggtaaaaca
      601 gctctactaa catataaaag tcccatgata tttgtatgta ccataacttc ccaatcttct
      661 atgttacctt catgtaatgg cgataaccca agagctaacc cggcattatt aaccaaaata
      721 tctatatttt gccaatcaat agtaagatta ctgaaaaaat taaaaacttc ttttttattt
      781 cttacatcta ctttatctat caatactttt acattactca gagttgaaat ttcttttttg
      841 atagattcta acttgtctat cctacgagct agcaaaatca aattgtcgcc atttatagca
      901 aattttttag cagtagctgc acctataccg ctactggcac ctgttattac aattgttcta
      961 ttcataaatg tttgatttta aattttttac aaattttttc tatatctata ataataacaa
     1021 aacttaataa taaaaataat aatggaaaaa tcctatatct gactatagcg cctacaatag
     1081 gagtagtata gcctattagt aaaaattcaa ttaatgcaaa taataaaaaa gaaaataaaa
     1141 tcaatttgtt atttctatgt aatgattttt tatgaaaaat aaatgtaaat atggcaaagc
     1201 tgatccacag tacattttct ataaaggcag gtataacaat tatattatgc aaatcagtaa
     1261 caaaaggcct aaataaagtg tttactaatg ctaatggtgc atttaaaata aatgaaaaaa
     1321 ttgtgggttt taaatttaat ggatttataa ggctaccagc attttcactt attgccaaat
     1381 tagtggctaa agttaattta ttaactacta tcattaatag gtccaaatta aatatccatt
     1441 tgatcagagg aatacataat atataaaata ataaaaaaac caagtatagt ttccaaattt
     1501 caattttttt caaaacttta gatattatta agggaaatat ggctaaaaac aatccaataa
     1561 ttatgtaaat tttaactgta aaaaataata tagttactaa tatgaatagg atgatagtaa
     1621 agatagaaac tggttttgta attattttat gtatactaaa aaaccataat cctaacaaaa
     1681 acattactag tgcttctttt aatactcctg acgtccacaa aactaaagaa ggaaagaaaa
     1741 aaataattat aaaagaaagt ttagcatttt ttatatatgg atttatggat ttaaaaaata
     1801 aaaattgacc tataaaagct aaaaaaacaa aaaataacat atgtagcaaa tagttgccac
     1861 ctgtcaaagg catcaatata gcatttatcc gtatcatcat tctgttatcg caagttatag
     1921 gcacataatt ggctctttcc caattgctag tatttattaa atatttgtct tttagtgtct
     1981 ggtattcatt tttatcataa attccaaaaa ttattttaat gaaatcattt gggttatctt
     2041 tgctagagtt ccataatatt ttgccgtcat cataatattt aaaaatatct gctttgtttc
     2101 tatcaaaata ataattagta taaataaata ttaaaaaaat acctgccaat atttttagaa
     2161 tgaaagctat catccaccaa actggggcaa tttcattgct atatctttta gttagaaaaa
     2221 aaataaatat cgtaaggaat atagctatta ttatagctat tactatttcc ataaataaaa
     2281 ttaatctaaa ttaatagcaa aaatacaaga aaattaataa atatttttcc taaattcttt
     2341 tagctcttct aaaaaattat tatctttttc tatatgattg cctataacga caccatttat
     2401 atcatggtta aaatattgat caataatatt taaattatta atcccacctc cgatgattat
     2461 aggtaatgaa ataatatttt tgagatttgt taatatttca gttttaatag gattgcaagc
     2521 accagaacca gcttctaaat aaattagttg agcgccagca tatttagctg ccaagcaagt
     2581 agctattaat atatcttttt tatcaggagg tataggtaag ctttgagtca tataagctgt
     2641 actactaata ttgccaccat ctattaaaat atatgctgta gatatagcag gtaaaccaga
     2701 tttataaata ataggcgcag ctattatctg ttggcttatt aaatattcag gattcctacc
     2761 agaaaaaagt tgtaaaaata aaatgccatc agcatcactt attatttgtg tgtaattacc
     2821 tgggaaaatt attactggta atgaagtttt tgattttaat gttattataa tatttctaaa
     2881 ccattcaata tcaaaaatca aactcccacc aattaaatat aaatcacaac agtcattatt
     2941 accagcgtag atatatttat ctaacaattc tttgtctgtt ttatcaggat caatcaatat
     3001 agctattgtt cgtgttttta aaaataaatt gtaaatttgt attgacattt ttgaaatttt
     3061 tatcaaaaat aataaattaa tgagaaaaat atttttattg tgtatgtttt tttgtccatt
     3121 acaattatta aattcacaaa atacgtatat tgttacatat gatactattt taaaacagga
     3181 aatactaata ggtagattaa ataaaaatga cttaatgaat tctgattttg ctgaatattt
     3241 ttatttatat catgaagctt ataaagctga agtggggata ataaataagt taaaacattt
     3301 aattaacgat gttaacatta caattgtcat gggtacttgg tgttcagatt ctcaagagca
     3361 aatacccaaa ttttataaaa taattaatct actagaatat gatgaaaata aaattttgtt
     3421 aataggtgta gataaaaaaa agcaagctag ggagtatgaa tcattggtcc agtcattaaa
     3481 catagaaaag atacctactt ttatttttta taaagacgaa aaagaaattg gtaggataat
     3541 agaaagtccc gaaacaacct tggaaatgga tatgctaaaa atattgaata aataataaat
     3601 attatcaatt taattcatac aattctctga aatttggtat taatgatttt aaataatcat
     3661 gcaatttttt tacttgtcct tccatgttac caaattgctt attaccacat tcataggtta
     3721 atcctttttt gatttcttta tttttaaatt ttatagttac tagataggtg acaccgccga
     3781 cgcagccttc ataatcatat ttggtttttt ctggaccaat ttgtatttta ttagcattga
     3841 aatatttgat tatttcattc cacgcatcag cagttatttt aacttttgtc ttatctaata
     3901 attcattttg tcttttaatt ttaagctgaa ttttattttt tttgataata aattcatact
     3961 ctctataata ttcaggagag aggggtgggg tttttaaaac aaaagttatt tccttaatct
     4021 ctggtaactg agcggataca ttttctatat tactgaataa aaaattagca attattataa
     4081 ataaaattaa tattttcgaa cttttcatat attatttatt ttaaattttt ctacaaattc
     4141 tttaaatgaa ataataggga taccaagttc attagctttt tggcgcttgg caggtcccat
     4201 attatcaccc gctatcaggt aatctgtatt actagaaaca gatgagagta ctttacctcc
     4261 attagcttct atcaatcttt tcagatcatc acgttctata tcttcgaata cacctgatat
     4321 tacaaaattt aatcctttca atttttctga ttttggtgtc tcttcatcta atgcaaattt
     4381 caagccagct ttttttaatc tttctattaa aagtagatta tctggatctg agaaccaatc
     4441 tatgattgac tgtgctgtta tatcacctac ctctggtatc tgttgtagct cttctttact
     4501 catttgcatc aatttatcta aagatttagc ataattagca atatttttag ccattacaga
     4561 gcctacattt ttaataccta gggcatacag cattctatca aatggtttag atttagattt
     4621 ttctatagat tttaaaatat tttctacagt tttgtgttga aaccgcacaa tgcgaatttt
     4681 tccatcaggc atctggtatt gattttctaa tcctaaaagt tgatcataag ttaaattata
     4741 aagatcagca ggagatttta ctagtccctt tctataaagt aattctattt tttcctcgcc
     4801 tagagtttct atatccatag cgtctttact aataaaatgt tgtaatttgc ctaagatttg
     4861 tggtggacaa aatttatcat tagtacaata tacgaatgga tcgccttctt tatgcaaagt
     4921 aggactattg caagccggac aaaaacgagg tgggcaaacg ggatatgcat caggaggacg
     4981 tttgtccact tctactttta caatcttagg aataatttct ccactttttt ctacccacac
     5041 atagtctttt acgtgaatat cattttgttt gacaaaatca aaattatgca gagtgactct
     5101 ctttactgta gtaccactga gtaatactgg atttagatta gccactggtg tgatgacccc
     5161 agtacgtcca acttggaaat ctacagaagt gatttgagtt agagcttgct ctggtttata
     5221 tttaaaagct actgcccagc gtggatattt agctgtattg ccaagctgac gttgtaattc
     5281 ataattatta attttaatta ccaccccatc tatgccaaat ggtagctcat cacgtttgat
     5341 ttcatattca tcgataaata ttttaacttc attcaaccca tgacatagct ttatatatgg
     5401 cggcactggt gctccccatt ctttagcttt catcattgcc tcgtaatgat tagagaaaat
     5461 aggagtctca gttaaaactt cataaggcat aaattgtaaa tttcttttag ctacgagaga
     5521 tgaatcttgt aattttaatg aaccagcggc agcattgcgt ggatttgcga aggggtattc
     5581 gctattttcc tctctttcct tatttaattt attaaaagaa tcaaagggca taataatctc
     5641 acctcgtata tctacccaat taggataatc tccaaaaagc ctcaatggaa catttttaat
     5701 agtttttaca ttattcgtta ccacttcacc tatagtgcca tcgccacggg taagagctgt
     5761 aatgagtatt ccattttcgt aaatacaact tatagctaaa ccatcatatt tgagctcagc
     5821 tacgaattct acatcatcta cattagtaat atttctttta aaaaaactaa ccacttcatt
     5881 ccaatcataa ctattatcta acgatagcat tggaaaacga tgtggctgat taggaaactc
     5941 cttagtgata aatccaccta ctctctgagt aggactatca ggctcttgat aattgtattg
     6001 tttctctaac cgctctagct ctttcagtag ttggtcatat tcataatcag aaatctctgg
     6061 tgaatttttt acataatata aataattatg cctatttagt tcagcagtta aatatttaat
     6121 tctatcaata gcttcttctt ttgtcataaa ttttttagaa aataataatt acaaatataa
     6181 aaattttatg gataatatat tttttattta atataaaaat tcaaaataaa caatacaaaa
     6241 atcaaaaaat cattttaaaa gtaaaaaaac tttttgttaa aaaaaatatt tcgcttagta
     6301 tacattatat tgaaaattga atttttttta agcatactct gctttcgctc attatctata
     6361 ttttgcacta gctcactcat atcatttttt acccacggtt accattatca ttttattcca
     6421 aggcacatat tctacaaatt tttctctaat ttcattttct tttgcttcat taataacata
     6481 aaaaaggttt tcataatatg ttttcatcga tgtacccagg tctatcattt ttagtttgtt
     6541 ttttatgata ttaaaagccc cgtcaaatgc aaacaagctt gtccccatta aatagtttct
     6601 tactctatcg agttcttcat tgctaatagt atcatttaca aatttatcat attctttttt
     6661 aatttcatca attacgaaat tagtttgttc agcttttact tcagcagata tactccatgc
     6721 agcttgatgc ttgaaatgat tagttctaca ataaatgcca taggtgagtc ctttatcttc
     6781 tctgatattt ttattaagtc tactaccaaa atatccacca aaaatagttt gacctatcaa
     6841 aaaatatgga taatcttttt cttttaatga ttttatctgg catcctattt taatagcagt
     6901 ttgtaaagaa tcttttttat taatatgtaa ttgtttttga gatggcatac cagcagataa
     6961 ttttaaatct tttatattta agtggggtaa aatatcgtaa tgttctgtaa taaatctaat
     7021 aatctcatca ttataattac ctgcaagccc ccagagagag ttagaaaaat cgaaatattt
     7081 tttttgaaat gttattaaat tttttctcga tgtcagtagt atatcagaat atttaatatg
     7141 tttaccataa ggatgattat tgccaaatag taatttgaaa aaatttttat tagctatgaa
     7201 gtctaccctt ttatttttga ttttcaaaat tcctaaggtt tcttgttttt gaagtattaa
     7261 ttctttttta ggatatgtag gatctgtaag tagctcaaac aaaagattta atgcatggat
     7321 aaaaagatta ttaagcgcaa tcaaagaaat attgactgaa tcgggtccta cagaaatatc
     7381 tattgaagca ccataataat ttatttcttc ggcaatttgg attgcagttt tttgtttagt
     7441 accagatttt atcatttccc aagcaaattt gcctatcaca ggattatttg caaatttata
     7501 tccaagaggg atgatcaggt atagagagac taaatctgta cgagtagtat gaaaagtata
     7561 gattttatta ttatcgctta gatttatttc atttacctca ggtattttag gtttagtagg
     7621 agtgattata tttggtcgca tattatttat ttttttaaat aatttaaaac aaaagcatta
     7681 tcaggtttta atatatcatc agccaaattc attatttcat cttttgtaat agatttataa
     7741 atttcataat tttcaaataa taaatctgca ctccctaatg cttcataaaa tgataactcg
     7801 aaacctatcc tttgcaaatc atatatattc attgagagtg tactcagata catatttctt
     7861 actttatcta attctttatc agtaacaggt tttttcctaa aattaactaa ttcattccac
     7921 agggtatcct cagctttttt aacatcaata gaaggtttta caaaaccaag tatcagcaaa
     7981 agcccaggat ctacactacc cataacactt gcatctattg aaataaaaag ctttttttct
     8041 ttgactaatg aagtatataa tcgcgacgaa ttcccattgc caagaatatc agataaaata
     8101 tcataggttg aatattgtgg tgagagccta ccaggcatag gtatagctat tacaattagg
     8161 tttaatggta cattgcgttt tactactttt ctcacttgat gtttgatagg tgattcaagt
     8221 ggataatcgc gttcatcaat aggtgtagat ggtatatctg agaaatattt ttctattagc
     8281 atttcgatat catcatgata tttaccacta atagctaaaa tagcattaga gggttgatac
     8341 catttatcat aaaatcgttt tacatcttct aaagttgctt tttcgatatg ttctggagtg
     8401 agacctattg taggccaacg atatggatgc ctagcatagc acatatcaaa gagaatatgt
     8461 aataaatctc cataaggctg attgagatga gtttgtttaa attcttcgat tacaacattt
     8521 ttttgaatat ttaagctttt ttcattaata ttcaagtgtt gcatcctatc actctcgagc
     8581 caaagagcta gttctatatt atatgatggt aattgaataa aataatctgt gaaatcttgg
     8641 gtagtaaatg cattattgat gcctccagct gactctactt cactatcata cgagggggca
     8701 tactctgatc cagcaaacat taaatgttcg aataaatgag caaagccagt tttgtctggt
     8761 tcctcgtcgc gtgagccaac aaaataaaaa acattaacgc atactaaagt agtatcctcc
     8821 acaggatata ggatatacct aaatccatta gataatttac ctttatgaaa agttttattg
     8881 agcataataa ataaattatc aacaaagata ctgaaaaata attgacattt gtttacaact
     8941 tttttttaaa atgatatttt tttgaataat atctacttaa tttacttttc tttgccagcc
     9001 acacaaagaa aactaacaaa agaaaggtct ccgctggttt taacacccct gtctctgtga
     9061 gacatcccct ctacaagagg agaatacgat gtgattcccc ttctccgaag gggtgtcagg
     9121 catagcctga cggggtagtt aaaaaaacta cgctggccag ccctacccaa ccctgcttgc
     9181 ttgttttatt acaaatcatt tcccaaaggc gggagggaca catttaaaga tgtgttttaa
     9241 gaaaaataat gatttctcta tgagacaaat agcttaccgt aaggaagata tcggatatga
     9301 aattttataa aataaaatta ataatatttt taatttttgt tttatttata ccaattatct
     9361 gtatttttaa ctttagtcca agtagattcc ctatttctct tttgtagctc atcttcattg
     9421 gcaaatctta caaatgccct attagcaaaa tcttttatat aaacaaaaac gttccccgct
     9481 gtatctacat ctaaacgtga tatttggtat ttcataaaat tttttgctaa atgttgaagt
     9541 tttatcgtat ctaattcaac ttctttatct gcgcatatcc aatgcatata tgtaatgttg
     9601 ttatcttgtg tatccatttt taaagtataa taaccaaatt ttaaagaatc attttttaaa
     9661 aagtctattc tacctggaat taatattttt ccactatcat cataacttct aatctccata
     9721 tttatacctt taaattttga aaaatcctca tattcatatt tatccaaaaa ttccttttct
     9781 cttttatgtt gctcacaact attatataat aaaagtatta tatgtaataa aaatattgca
     9841 aaaatgatga atatcaaact ctttattatc ttcataatat ttaatttttt attaaaggat
     9901 gaacttctac tctgtaatac atatctttat atttatactt gtttgcatgt cgaactccca
     9961 tatatataga aagttgatca tctcgtgaat ctatttgagg tttgtatcca tttgtattag
    10021 aattaaataa actattgtaa tgtgctccca ttagcaattc tggtaaagta aaaccaatag
    10081 ctttgcctgc tgctccgaat aaaaagttcc caaaattcat atcattatga gctactccat
    10141 ctactaaaaa taatgtttgt tctttggtta tatttggata ttgatatttt aatccccatt
    10201 cttcggttgt agcaaaatca aatttccctc caccttttcc ttcctctcca atgtagtcat
    10261 atctattaaa caagtcgtta cttgcggttt tattttcttg attaaatgcc cctgctctag
    10321 atagcatact tataatctct tcttcttgaa caaaatgtat tttatttatt tttccattta
    10381 ttaagtcatt tacatcattt ttaggatcgg caaatttaaa tttttgtgta acattaccat
    10441 cgctatcaac aatacgtcct gtacgtccga aaaatgtatt atgaaaccaa ttatcttttt
    10501 tcgttcccaa ataattacca ttttcatcaa attctgatat acttgtccca ttcggatcca
    10561 ccagcactac aggattatca gctgtatagc aatatggcga tagcgatgga tatttatcgc
    10621 tcatcggatc tacgctcagc catccactca gctcgctatc ataatagcgt gcttcgaagt
    10681 atgtgtagct ggtctcgttg tctagctctt tggcagtgaa tttgtaccta ctatcaaact
    10741 cttcagtgtt tcgctgggag acgaagagct cgccgtaggg gagatactgc aaatgctgca
    10801 ctgctgcacc ttctgcatta gttacgaaag aggcggagcc gagatggtcg gagtggtagt
    10861 ggattttcat ttataaattt ttgttaggta aaattaatga atattttgga ataaatttct
    10921 acttaattta cttttctttg ccagccacac aaagaaaact aacaaaagaa aggtctccgc
    10981 tggggaaatg atttgataaa actacgcaat cacctcatcg tcatttaggc aattcttgaa
    11041 ttgcctctac aataactgta tttgcaaatc atttcccaat gagggcggga taaaataaaa
    11101 acacaaagca tgcaattaaa tatatttgat tatgaaatta ttcttaaaaa cttacaaata
    11161 tacttttatt taattacaaa aactagactt accttcaaaa ataaattcac cattaatata
    11221 tatataagac cattgagaaa aagaatcatc tataatgaaa tctaactcta aaattttgtt
    11281 tttatcatat tttcgtttgg tattttgttc tataatataa tctaaatctt tttctaaata
    11341 tttgaatgat accttttgtc cacaaataga aaataattct tctaaactat ctccatccac
    11401 aataaacata tgatttttat aataaaagaa accatatgga tctaacccta ata
//
LOCUS       DAOPRN010000061        10986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_5011_length_10986_cov_110.909981, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000061 DAOPRN010000000
VERSION     DAOPRN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5011_length_10986_cov_110.909981"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            510..1631
                     /locus_tag="PKI65_06855"
     CDS             510..1631
                     /locus_tag="PKI65_06855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB78195.1"
                     /translation="MNKVCHLTSVHQRNDIRIFHKECKSLGRYNYDVWMIVADGKGDE
                     IIDGINILDVGKSKTRLQRMLITTNKIYKKALQLNADVYHFHDPELIPIGLRLIKKGK
                     KVIYDVHEDVPRQLLSKPYLNKTTLKIISNIFKKYEEKNAKKFSAIITVVPEITQRLG
                     EINKKTIEVRNYPMLSEFPFINTNWQDREDAMVYIGGISEIRGINEMLQIAYHSQIKL
                     HLAGPFSPPELENKIKTKEEWKYVIYHGVLNRKEIVNLLLSVKLGLLLLHPVPNHIIG
                     LNTKVFEYLAAGIPVITSDLPNYKNIIIDNNCGFCIDIFDTNEIVEKVKYIFMHDREA
                     EMMGQSGRKAVETIYNWENEEKKLIELYENIILTDEFAL"
     gene            complement(1780..3519)
                     /gene="aspS"
                     /locus_tag="PKI65_06860"
     CDS             complement(1780..3519)
                     /gene="aspS"
                     /locus_tag="PKI65_06860"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOB78196.1"
                     /translation="MFRSHNCGELRIKDLDTQVTLCGWVHHIRDLGKLLFIDLRDRYG
                     TTQIYFDSNNKPQLYQIAKNLGREYVIQIEGIVRERSNKNPKMPTGDIEIDVDRLTVL
                     NKSKIPPFTIEDDTDGGEELRMQYRYLDLRRNVMKETMIFRHELNLSIRNFLSEKSFL
                     EIETPFLIKSTPEGARDFIVPSRLNPGNFYALPQSPQLLKQLLMFAGMDRYFQIVRCF
                     RDEDFRADRQPEFTQLDCEMSFVEREDILDLFEDMMRYLFNKLLNIDLPTFPRLTYQK
                     AMETYGSDKPDLRFDMPIIDITDKLKGHGFKFFDDSEYIGGLLLINKPSVSRKYIDKL
                     TEWVKSPQIGSTGMAYIRYLDGELKASISRFFSNEDLQKIMNSLKAGNNDILFIISGN
                     KEKSLNILGNLRLKLANDYQLIQKNTFKPLWVIEFPMFEWDDENQRYTASHHPFTSPL
                     NEDIELLDTNPAIARSNAYDMVINGVEVGGGSIRIHDKNLQKKIFKLLNLTNQEAEDK
                     FGFFLKALDFGAPPHGGIAFGIDRILTVMLGLNSIRDVIAFPKNNMGRDLMMQAPAPI
                     SEEQLKELHIKII"
     gene            complement(3512..4573)
                     /locus_tag="PKI65_06865"
     CDS             complement(3512..4573)
                     /locus_tag="PKI65_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HOB78197.1"
                     /translation="MEEIITLISFVILVAFLMFIDLKVGKTQHHVMTLKSAALWTIFW
                     IGAGVLFGFSLLLWGHHIHSIHSIEDLSFIIKKHHHLININGLAFDEALKLYRQNLTV
                     EYFTGYFIEKSLSLDNIFVWLIIFMSFNVPEKYYKDILFWGIVGAVVFRFIFIFGLSS
                     LLTAFEWVLFIFAAILLYTGIKMIFKKKDNGEKEKEKYQNHFIVRQFSKFRHFYPDYD
                     RGFFFKKGGFIFVTGSFLVLCIVEFTDIIFAIDSIPAIFAITADPYIIYFSNIFAILG
                     LRALFFLVYHGLRSLYYLNYGLGTLLILMGVKIITEDWLHEIGFTNIHSLILVLSIIT
                     ITIVASLLKKNNSKVKSNV"
     gene            complement(4877..6421)
                     /gene="nadE"
                     /locus_tag="PKI65_06870"
     CDS             complement(4877..6421)
                     /gene="nadE"
                     /locus_tag="PKI65_06870"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOB78198.1"
                     /translation="MNNIQFINLTSHPYNIVDNTNNIIKIVNQAPMIDDSLYLLPPFC
                     LRNYNTANINDKHQNDLLNESLNRLSQELNKYVLISYPTKIKDCLLDVYYYILGNGSK
                     KFIGNLNFELNHYQKQPLQKLNFIYNNKSYTILPYLDEIHELCDENIILQDIKPFSYQ
                     HYLNVLDDFEKINFPINIIYQSINSFQSGNLYPGLVAVRSNNENYYSELYPAEGVLDI
                     DKFSKFKDTDKNFDSIIYKSLIVGLNRFMKINKFNDVVIGISGGIDSAVVATLATEAL
                     GADHVIGLIMPSMFTTSLSITDAEQLSNNLKIDYYILPITSIYNTTLDVLKPVFKKTK
                     FSTAEENLQARIRMLLLMAYSNKFGHIVLNCSNKSELSVGYGTLYGDLAGSISVIGDL
                     YKTQVYQIANYINEINTKEIIPKSIITKAPSAELHADQKDEDTLPPYDLLDLILYNWI
                     EENRWSESTEKLMHKLDLDISNIEIKKWENLKKIISANEFKRNQLAPIIRLSHKPLNE
                     VSFSFI"
     gene            complement(6800..7210)
                     /locus_tag="PKI65_06875"
     CDS             complement(6800..7210)
                     /locus_tag="PKI65_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78199.1"
                     /translation="MNTIDSINLLKIEKILKEYGWPSTELVGEQGVNTIFLIIQHANA
                     KARNNYSKLLKKAARKDISQRPNYAYLIDKIKMDKGKKQIYGTQLKYVEEKKCFELFP
                     IKNIKNVDKRREKMFLPNLDEYLKLIEEYYNLCK"
     gene            complement(7207..7746)
                     /locus_tag="PKI65_06880"
     CDS             complement(7207..7746)
                     /locus_tag="PKI65_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78200.1"
                     /translation="MKKNFLTFIGTFLLFITLSYSQDTSEYIVDDHVYSLFEQGQYDS
                     VIKYINKHYPMPKDEFDLPYFAHLELKAKCFVLLDQIDSAFNVLFNLTLPIHEDIIYD
                     KDFKKLQSDPRWEQWLNIFIEKNIINDNIEKKDLAKELFLIEIEDQMIRTELLDLISS
                     AESYNQEELSSIIDSFAKK"
     gene            complement(8056..8478)
                     /locus_tag="PKI65_06885"
     CDS             complement(8056..8478)
                     /locus_tag="PKI65_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOB78201.1"
                     /translation="MEKITKYCVIQERCKYEVINKLKVYKVNIDDIDKYIIYLEQNNF
                     LNEDRYTELFIRSKINQNQWGPIKIKFALYQKNIPAPLINKHFANYDENFFKQKIYDY
                     LKKHQIVIEKLSYQEKIYWARHLLQKGYDSDMISEILF"
     gene            complement(8495..10051)
                     /locus_tag="PKI65_06890"
     CDS             complement(8495..10051)
                     /locus_tag="PKI65_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOB78202.1"
                     /translation="MNDVIRELFIDKRHNKVRIALTENNKLVELQEENESDLFAVGDI
                     LLGRVKKILSGPNAAFIDVGYSKDAFLQYYDLGDNFLTLQKYTHELISGKNVSLEYYK
                     DCKQLPKSGSINKVLKQGDVVLIKVTKEPISNKGPRTTAEISLAGRYLVLVPFQNKIS
                     ISHKITDNEEKKRLKEMAETLRPYRFGLIVRTAAQDRSFSELQNDLEDLLNKWKNIEK
                     NLKHASPPKIVYSELDRSLSVVRDILNDSFSSITVGDVNLYRDITDYLEKHSPDKLDI
                     LKLHKGTNSLFETVGLEKQIKQSFGKKVTLKSGIYLIIERTEALQVIDVNSGYKSNDN
                     KNQEENALEANMEAAEEIARQIRLRDLGGIIIIDFIDMHSSENRRILYDRLKELMKSD
                     RAKHTILPPTEFGLVQITRQRVRPELNIDIDELCPLCEGTGQIKPSILIIDNIFDNIS
                     YILSTQPAKKLSLYLSPYLYTYIKYGLPSIYLKWKLKFKNKIKIFKNDDYHFLEYHIY
                     DYDGEEISLQ"
     gene            complement(10204..10500)
                     /locus_tag="PKI65_06895"
     CDS             complement(10204..10500)
                     /locus_tag="PKI65_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOB78203.1"
                     /translation="MTKADIIKDIQKKTNLSRDLIKLVIDSFMETVKENMSQGENIYL
                     RGFGSFLLKERKEKKARIISKNETIIVPAHYKPVFKPAPEFLKSVKENLTIENN"
BASE COUNT         3839 a         1477 c         1390 g         4280 t
ORIGIN      
        1 ttgatataga ttatctagaa atctacgttt ctgctctttg ggtagagctt gataagctaa
       61 taaattaaaa ttatccataa ttattttttt gtaaaattaa ataaaaatta tttaatggta
      121 gtataaaata cgtaaagtaa aacaaaatta taattttcct attaaattaa gcattagctt
      181 accattaatt atttttaata aatttattaa aaaatgtaaa aaaaaaaaga aacttattaa
      241 aaattattat ttttgtactt aaagtaaaaa agtataattt ttaataatat tagtaatcaa
      301 tttatattac atctcaaatg ataaagtagt taagttaaat aaaatataat tataataatt
      361 agttaacaat tacattaatg tattagagct tgtaaaataa ttttttttaa aaatataaaa
      421 ataatagata agtaaaaaat aaagcgaaat attttcggtt tttttatcct atgattaata
      481 agatcataaa ttagtttatt ataaaagata tgaataaagt gtgtcatttg acgagcgtgc
      541 atcagagaaa tgatataaga atctttcata aagagtgcaa aagcttaggg agatataatt
      601 atgatgtttg gatgatagta gcagatggta aaggtgatga gatcatagat ggaattaata
      661 ttttggatgt gggtaaaagc aaaactcgat tacagcgtat gctgataact acaaataaaa
      721 tttataaaaa agctttgcaa ttaaatgccg atgtatatca ttttcatgat cctgagttga
      781 tccccatagg cttgagattg attaaaaaag gaaaaaaggt aatttatgat gtacacgaag
      841 atgttcccag gcaattattg agtaaaccat atctaaataa aactacatta aaaattattt
      901 caaatatttt taaaaaatat gaagaaaaaa atgcaaaaaa attttcggct attatcactg
      961 tagtacctga aataacgcaa agattgggtg aaataaataa aaaaactata gaagtaagaa
     1021 attatcctat gctaagtgaa tttcctttta taaatactaa ttggcaagat agagaagatg
     1081 cgatggtata tataggagga atatcagaga taagagggat aaatgaaatg ttgcagatag
     1141 cttatcattc gcagataaag cttcatctag caggtccttt ttcacctcca gaattagaaa
     1201 ataaaataaa aacaaaagaa gaatggaaat atgtgattta ccatggtgta ttgaatagaa
     1261 aggaaatagt aaatttacta ttatcagtta aactcggctt attattatta catcccgtcc
     1321 ctaatcacat aattggcttg aatacaaaag tttttgaata tttagctgca ggtatacctg
     1381 taataacttc tgatttacca aattacaaga atatcattat agataataat tgtggttttt
     1441 gtatagatat attcgataca aatgaaatag tagaaaaagt caaatatatt tttatgcatg
     1501 atagagaagc tgaaatgatg ggacaaagcg gtagaaaggc tgtagaaaca atttataact
     1561 gggaaaacga agaaaaaaaa ttgatagagc tatatgaaaa tattatatta actgacgaat
     1621 ttgctctatg aaattcattt tgtaggaggt tgaccagcat ttcatagggt aaataacata
     1681 caattaacaa tgaatacttt taatattctt aatacattaa tagataatat tagatttttc
     1741 aaatctattt atttatgtaa ggctcaaatt taaatatatt tatatgattt ttatatgtaa
     1801 ttcttttaat tgttcttcgg atataggtgc tggagcttgc atcatcaggt ctctacccat
     1861 attattttta ggaaaagcta ttacatctct aatactattc aaacctagca taacagttaa
     1921 tattctatct attccgaagg ctattcctcc gtgtggaggt gctccaaagt ctaatgcttt
     1981 taggaaaaat ccaaatttat cctccgcttc ttgattagtc aagtttaata gtttaaatat
     2041 ctttttttgt aaatttttgt catgaattct gatagaacca ccgcctactt ctacaccatt
     2101 tattaccata tcataagcat ttgatctagc aatggcagga ttagtatcga gtaattcaat
     2161 gtcttcattt aatggacttg taaaaggatg atgagaagct gtgtatcttt gattttcatc
     2221 atcccattca aacattggaa attctataac ccacaaaggt ttgaaagtat ttttttgtat
     2281 taattgataa tcattagcta attttaaacg taaattacct aatatgttca atgatttttc
     2341 tttgttacca ctaatgataa ataatatgtc attattacca gccttgagag aattcattat
     2401 tttttggaga tcttcattag aaaaaaatcg agaaatggat gcttttagtt ctccatctaa
     2461 atatctaata taagccatac cagtactgcc tatttgtgga ctttttaccc attcagttaa
     2521 tttatctata tactttcgtg atacagatgg tttgtttatt agtaataaac cgcctatata
     2581 ttctgaatca tcaaaaaatt taaaaccatg tccttttagt ttatctgtaa tatcgattat
     2641 aggcatatcg aagcgcaaat cgggcttatc actgccgtaa gtttccatag ctttttgata
     2701 tgttagtcga gggaaggtag gtaaatcaat attaagaagt ttattgaaaa gatatctcat
     2761 catatcttcg aatagatcta atatatcttc tcgttctaca aaagacattt cgcagtctag
     2821 ttgtgtaaat tctggttgtc tatctgcgcg gaagtcttcg tctctaaaac agcgtacaat
     2881 ttgaaaataa cgatccattc ctgcaaacat cagaagttgt tttagtaatt gaggtgattg
     2941 aggtaaagca tagaaattac ccggatttaa tcttgaaggt acgataaaat ctcttgctcc
     3001 ttcaggagta ctttttatga gaaatggagt ttctatttct aaaaaacttt tctcgcttag
     3061 aaaatttctt attgataaat tgagttcatg cctaaaaatc attgtttctt tcattacatt
     3121 acgtcgtaaa tcgaggtatc tatattgcat tctcagctct tcgccaccat cagtatcatc
     3181 ttctatagta aaagggggaa tttttgattt attcagtact gtcaatctat ctacatctat
     3241 ttctatatca ccagtaggca ttttaggatt tttattactc ctctctctca ctatgccctc
     3301 tatttggatt acatattctc ggcctaaatt tttagctatt tgatacaatt gaggtttatt
     3361 attagagtca aaatatattt gagtagttcc atagcgatct ctaaggtcga taaataataa
     3421 ttttcctaaa tctctgatat gatgcaccca tccacacaaa gttacttgag tgtctaaatc
     3481 ttttattcta agttctccac aattatgact tctaaacatt tgattttact tttgaattat
     3541 tcttttttaa taatgatgct acaatggtaa tagttataat acttagaact aatatcaagc
     3601 tatgaatatt agtaaaacct atttcgtgta gccagtcttc agtgattatt ttcacaccca
     3661 tcaaaataag taaagttcct aaaccgtaat taagataata taaactgcgc aatccatgat
     3721 ataccagaaa aaataaagct ctaagtccta aaattgcaaa aatattagaa aaataaatta
     3781 tatatggatc tgcggtaata gcaaaaatgg caggtataga atctattgca aagatgatat
     3841 ctgtaaattc gactatgcat aaaactaaaa aactacctgt aacaaatata aatccacctt
     3901 ttttgaaaaa gaaacctcta tcataatctg gatagaaatg acgaaattta gaaaactgcc
     3961 ttactataaa atggttttga tacttttctt tttctttttc tccgttatct tttttcttaa
     4021 aaatcatttt gataccagta tatagtaata tagctgcaaa aataaaaagc acccattcga
     4081 atgcagttaa aagtgatgac aaaccaaaaa taaagataaa cctaaatact actgctccca
     4141 ctatacccca gaataaaata tctttataat atttctctgg cacattaaaa gacataaata
     4201 ttattaacca tacgaaaata ttatctagtg atagagattt ctctatgaaa tatcctgtga
     4261 aatattctac tgtaagattt tgtctatata atttcaatgc ttcatcaaag gccaaaccat
     4321 taatatttat taaatgatgg tgctttttga tgataaaaga taaatcttct attgaatgaa
     4381 tcgaatgaat atgatggccc catagcaata agctaaaacc aaataataca ccagctccta
     4441 tccaaaaaat agtccatagc gcagctgact tcaatgtcat cacgtgatgt tgtgttttac
     4501 ctactttcag atcaataaac attaaaaaag ctacaagtat gacaaagctt attagtgtaa
     4561 ttatctcttc catttttcta tttcattgtg caaaaataat tgaaattttt gattttttgt
     4621 ttttttttta actaaataca ttttttttta gcaattcata aattgcatga atttttattg
     4681 aattaggctc ataactaata taaatttttc aataaattct tttttatttt aattttctta
     4741 gtcaattatg caaaaaaaac taacaaaagt taaatttgtg caagtaaaaa tggtttgaca
     4801 aataatttag agatagatgt aaaattttat aatcgttcat caaaagtata attaggtatt
     4861 ttttttatta ttcagattaa atgaagctaa atgaaacttc atttaatggt ttatgtgata
     4921 atctaataat gggtgctaat tgatttcttt taaattcatt tgcgctaatt atttttttca
     4981 agttttccca tttttttatt tctatatttg aaatatctaa atctaattta tgcattaatt
     5041 tttctgttga ttcactccat ctattctctt ctatccagtt atataatatt agatctaata
     5101 aatcatatgg aggcagagta tcttcatctt tttggtcagc gtgtagttca gcagatggtg
     5161 ctttagtaat tatagattta ggtatgatct ccttagtatt aatctcatta atataattag
     5221 ctatttgata tacttgggtt ttatataaat cacctattac agatatagag cctgctaagt
     5281 ctccatataa tgtgccatag cccacagata attcactttt attgctacaa tttaaaacta
     5341 tatgaccaaa cttattagaa tatgccatta ataaaagcat tcttatacgt gcttgtaaat
     5401 tctcctctgc agtagaaaat ttcgtttttt tgaaaactgg ttttaataca tcaagtgtag
     5461 tattatatat agaagtaatg ggtagaatat aataatctat tttcaaatta ttagataatt
     5521 gttctgcatc agtgatagat agtgaagtag taaacataga tggcatgatc agtccaatta
     5581 catgatctgc tccaagtgcc tctgttgcta gtgtagctac tacagcgcta tctataccac
     5641 ccgatatccc gattactaca tcattaaatt tatttatttt cataaatcta ttcagaccta
     5701 ctattaaaga cttataaatg atactatcaa aatttttatc tgtgtcttta aattttgaaa
     5761 atttatctat atctaatacg ccctctgctg gatataattc tgaataataa ttttcattat
     5821 tgcttctgac agctacaagt cccggatata gattgccaga ttgaaaacta ttgatactct
     5881 gataaattat attaatagga aaattgattt tttcgaaatc atctaaaaca tttaaataat
     5941 gttgatatga aaaaggttta atatcttgta atataatatt ctcatcgcat agctcatgaa
     6001 tttcatctaa ataaggtaaa atagtgtatg atttattatt ataaataaaa tttaattttt
     6061 gtaagggttg cttttgataa tgatttaatt caaaattgag attgcctata aattttttag
     6121 atccattacc taaaatataa taataaacat ctaatagaca atcttttatt ttagtagggt
     6181 aagaaatcaa aacatattta ttaagctctt gtgatagtct atttaatgat tcatttagca
     6241 aatcattttg atgtttatca ttaatattag cagtattata atttctcagg caaaatggtg
     6301 gtaataaata taaactatca tcaatcatag gcgcctgatt aacaatttta ataatattat
     6361 ttgtgttatc tacaatatta tatggatgag aagttagatt aataaattgt atgttattca
     6421 taagtttata aatatttcca agtattttta gcaaaattac gattattttt tttaagaaaa
     6481 tttaatttta aaatagtaat ttattgttat tttttagatt attataagta aaattttaat
     6541 aatttactta ttagcatata tcatatttgc ataaattatc aatctaataa ttttaattaa
     6601 taaaatattt gagtatgact gatacccaaa tcaataaaaa atatactaaa agcatttata
     6661 ttataacatt tgtattttta ttaatgcata tagatttatt gagctaatga taagacttta
     6721 ggtttttata tattaaatgc ttgcaatata tactgcaagc atttaataca agctaaatat
     6781 cttttttgat ttattattat catttacata aattgtagta ttcttcgatc aacttcaaat
     6841 attcatctaa attaggtaag aacatttttt cacgacgttt atcgacattt tttatatttt
     6901 taattggaaa aagttcaaag cattttttct cttcgacata ttttaattgt gtaccataaa
     6961 tttgtttttt tcctttatcc atttttattt tatctatcaa atatgcataa tttggtcttt
     7021 gggaaatatc ttttcgagct gcttttttta gtagtttact ataattattt ctagctttag
     7081 cattagcatg ctgaataatt aaaaatatag tattgacacc ttgttctcct accaattctg
     7141 ttgaaggcca tccatattct ttcaaaattt tttcaatttt taataaatta attgaatcaa
     7201 tagtattcat tttttagcga atgaatctat tatacttgat aattcttctt ggttataact
     7261 ttcagcagaa gatattaagt cgagtaattc tgtacgaatc atttgatctt cgatttcaat
     7321 taaaaaaagt tctttagcta aatctttttt ctcaatattg tcatttatta tgtttttttc
     7381 gataaaaata ttgagccact gttcccacct aggatctgat tgtaattttt tgaaatcttt
     7441 atcataaata atatcttcgt gtatgggtaa agttaaatta aataaaacat taaaagcaga
     7501 gtctatctga tctaataaaa caaaacattt agcctttaac tccaaatggg caaaataagg
     7561 taaatcaaat tcatcttttg gcattggata atgtttgttt atatatttaa ttactgaatc
     7621 atattgacct tgctcaaata gagaataaac atggtcatct acaatatatt cactcgtatc
     7681 ttgcgaatat gataatgtaa taaataataa gaacgtaccg ataaatgtta agaaattttt
     7741 tttcataatt ttttggtttt atattttact tattagtaaa acgttttaat aattaaaaag
     7801 ttacactttt ttaatcatat tagttatgat aaatataaat tctaaataac gatttaaatt
     7861 aatcgaattg taattttgta aaaatattac aatgtgaata aaaaacaatg taaaaaaaat
     7921 atttattaaa aaattataaa cagataactc tataaatttt ttaaacttct acttttcgct
     7981 ttctctatct ttgcctctaa tttttcactt tactctgata ctagctttac tttgtaattt
     8041 gatattttat ttattttaaa ataagatctc gcttatcata tcgctatcat atcctttttg
     8101 taataaatgc ctcgcccaat aaattttttc ttgatacgac aatttttcta ttactatttg
     8161 atgttttttt aaataatcat aaattttttg tttaaaaaaa ttttcatcat aatttgcaaa
     8221 atgtttgttt ataagtggtg caggaatatt tttttgataa agagcaaatt ttattttaat
     8281 aggtccccat tgattttgat ttatcttgct acgaataaaa agctctgtat atctatcttc
     8341 atttaaaaaa ttattttgct ccagataaat aatatactta tctatatcat caatgtttac
     8401 tttataaact tttaatttat ttattacctc atacttgcat ctctcttgta tgacacaata
     8461 tttagttatt ttttccaaaa ttttgggatc catattattg taatgagatc tcctcgccat
     8521 cataatcgta aatatgatat tctaggaaat ggtaatcatc atttttgaaa attttaattt
     8581 tatttttaaa ttttaatttc catttaaggt aaatagaagg taaaccatat ttgatataag
     8641 tatacaaata agggctcaga tataaagata atttttttgc aggttgagta cttaatatat
     8701 agctaatatt atcaaaaata ttatcaataa ttaaaatact cggttttatc tgtcccgtac
     8761 cttcgcatag agggcaaagt tcatcgatat caatatttag ttctggtcta acgcgttgtc
     8821 gagtgatttg tacaagtcca aactcagtag gtggcaagat agtatgtttg gccctatcac
     8881 ttttcattag ttcttttagt ctatcatata atatccgtct attttcacta gaatgcatgt
     8941 caatgaaatc aataataata atgcctccta gatctcttaa gcgaatttgt ctagctattt
     9001 cttcagcagc ttccatatta gcttctaatg cattttcttc ttgattttta ttgtcattac
     9061 ttttataacc actattgaca tctattactt gtaaagcttc agtacgttct attattagat
     9121 aaatacctga ttttaatgtt acttttttac caaatgattg ttttatttgt ttttctaatc
     9181 ctactgtttc aaacaatgaa ttagttcctt tgtgcagttt gagaatatct aatttgtcgg
     9241 gagaatgctt ttcgagataa tcggtaatat ctctataaag atttacatct cctacagtta
     9301 tagatgaaaa gctatcattt aatatatctc taactacaga taatgaacga tctaactcag
     9361 aatacactat tttaggaggc gaggcatgtt ttaaattttt ttcaatattc ttccatttat
     9421 ttaataaatc ttcaaggtca ttttgtaatt cagagaagct acgatcttga gctgcagtac
     9481 gaacaatcaa tccaaatcta tagggtctaa gcgtctctgc catttctttt aatcgttttt
     9541 tttcttcatt atcagttatt ttgtgactaa tagaaatttt attttggaaa ggtacaagaa
     9601 ccaaatacct tcccgcaaga cttatctcag ctgttgttcg cggtccttta ttacttatag
     9661 gttctttagt tacttttatc aaaactacat caccttgttt taaaacttta ttgatactac
     9721 cagacttagg taattgttta caatctttgt aatattctaa agaaacgttt ttaccactga
     9781 taagctcgtg agtatatttt tgcagtgtta aaaaattatc tcctagatca taatattgta
     9841 aaaaagcatc tttactataa ccgacatcaa taaatgcggc attaggacca gagagtattt
     9901 tttttactcg tcccaataat atatcaccta ctgcaaataa atcactttca ttttcttctt
     9961 gaagctcaac gagcttatta ttttcagtaa gtgctattcg gaccttatta tgtctcttgt
    10021 caataaaaag ttctcgtatg acatcattca tctttacagt ttatacataa aaaattaaat
    10081 ttgtttaacg aatctttttc ttatgtctgt tttttctcaa tctttttttt cttttatgtg
    10141 tagctatttt acgttttttt ctttttcttc cacacggcat aattttatct attttttaaa
    10201 aaattagtta ttttctattg ttaaattttc ttttacggat tttaaaaatt caggagcagg
    10261 tttaaaaaca ggtttataat gtgctggcac aatgatagtt tcatttttac ttattattct
    10321 tgcttttttt tcttttcttt cttttaataa aaaagaacca aagcccctta aatatatatt
    10381 ttcaccttga gacatatttt cttttacagt ttccataaag ctatctatta ctaattttat
    10441 cagatctcta cttagattag ttttcttttg tatatcctta attatgtcag cttttgtcat
    10501 aatttattat tttagaattg caaaattaca ttaaaaatat taattaacaa aatcattatt
    10561 ttttaaagtt ttaggaaata attaaaaaaa aagctgatta tcctgatact tttcttagca
    10621 cagcagaaat atgtcaagca ttaggtgaat atagtgtaat tatttctaat ttttcttttt
    10681 cacatcctat gtctgaggct tgtgcagtag cagtattaac tgacacttgg atttcaacta
    10741 atcaagttaa cattaaattt attattaatg atcacattaa taatagatta atctttattt
    10801 ttgattatac atgttctagt ggttttggta gtaattcatc attattagta gattatttaa
    10861 taattttttt ttgagtattt ttttgagtat taggaggtta atgaaaggat tgatgggaat
    10921 caaaataatc ggaaattaat aaatgtttaa gtttttcaag tttatatgtt aaataatttt
    10981 tatatt
//
LOCUS       DAOPRN010000062        10485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_5332_length_10485_cov_116.103356, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000062 DAOPRN010000000
VERSION     DAOPRN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5332_length_10485_cov_116.103356"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..2609)
                     /locus_tag="PKI65_06900"
     CDS             complement(321..2609)
                     /locus_tag="PKI65_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78204.1"
                     /translation="MNKYWQIIISVIIVLLIGLAIYNFPNLMIHKYNVSDIIDSTFDE
                     IWLIDKSAYEFYNSDTSLYLNVFPDFFKNSSSWQNYFKKIYIINDNDKIYWILYANID
                     YKKNKIIKWFEQLNFKKIKNKYYQNLNDEILYLKYFPYVLYAKDEKSLPIGEGYDKYS
                     DEYFTLNSNYNLHLALNTSKLKFINKKWAQWGIKFLTDIEQIYYFDINNQDDLWIFYQ
                     KFPKEHLISSSEYDFDLSIIPANMEQFFVISKISDQPLSNALQPYIIKSLNNHIFFDN
                     VDFSNMIKPHVLVGIIDQNPLLIMQCKDTTNIINQLNNKSELISKNTWMCTENIFNNT
                     KKSSHSLSYLFIKDTLLFYFEEIKLLELLNKSYFPITQKEFYKKISAYLRSEINELAF
                     FNCFSPPFSFYKNKDLQGQLWMINNNDLTTQKIFIWIEKIKNSQSNNKIILYNKICKQ
                     CKDPKLIYSPSNVYITYIQDKKIFIEGLITKSFVEIDLPDKNIIDALVMDDGKEHLLL
                     VSPSGLFMYDMAGNLKWKKQANILGYALFKYEDGLQRLFYLSNDGILHNLNSDGNTPE
                     GWKLIKTSNNSRVAYAKWNNKEYICVADNNNLTLYNRRGDKQAHYKFNTPLKDLHFSQ
                     KNEILYLIDIQGKIYELREAKQGSLVGKPTGFNNFTLIGDTLVMWDKYNIAWIDKDNG
                     KLVRDFKYQGNIIAKDNNLSWIKKTSPLIIKDHDNTFTILSAKMKILDTLSHIESIDA
                     LYWNNSYLLLTKTDGVIETIKR"
     gene            2768..4660
                     /gene="yidC"
                     /locus_tag="PKI65_06905"
     CDS             2768..4660
                     /gene="yidC"
                     /locus_tag="PKI65_06905"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOB78205.1"
                     /translation="MDRSQVIGFILLFALLIGYMFYLSPSKEELERQKQIQDSLRLVE
                     QQKKDSIFLAEQAIQESAKDTIKTESDIDTTYIVESQKSIPEAYTFFKQSYQTEDSII
                     FIENDVAIISFYCKGAILHSVKLKNFKDYQNNPVQLIDTNNYEFSFVFRAFDRFLETK
                     NLFFEPSIKNSNGNIYVTFIANARDTLTNEILGGVKIEYSIPQNEYMVDYKLSFINPD
                     EIGVLPLDILTFSWANNMIQQEEDKKVETINTTICYMNSEKKVKHLKAKDQKQTLNFR
                     VKWIGLKQQFFTHTLINVEDYFSSAELETKSFSPDDTSRYLRRMSVGMTIEHSTDRPV
                     HFKIYTGPLKYSILRSYKLRLEREIPLGWGFAPIAWVNRFLVIPVFNWLERYGLNYGI
                     IILILTLLLKIVLLPFTYKTYMSTAKMRALKPELDKINQKYSKPDDALKKQQATMELY
                     KKSKINPAGGCWPMLLQFPILIAMFRFFPSAIELRHKPFLWAHDLSASDSILDLGFNI
                     PLYGDHVSLFALLMAISTLLYTYINNKLTPTDTNAPGMKFMMYAMPIIFLGIFNNYSS
                     ALSYYYFLFNVVSILQTLTFRYFVNEEKIRAKLLENWKKPVKKSSWQQRMEELAKQQQ
                     TNVKKR"
     gene            4807..5583
                     /locus_tag="PKI65_06910"
     CDS             4807..5583
                     /locus_tag="PKI65_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78206.1"
                     /translation="MLDYYLDLMIKDENISTIKLLLEEIESLHSVVLKEYINLLPLDN
                     IADRIIDIDDELIIDTDLDDDAKTIDSIINDFDDIEIKIEEELEIEHHITESEKIDEL
                     NLENEQIDVEFNQDKIDLKEEIIDYESISEESEQYLSNDIKINIKTEQTEQKTIFESF
                     INGSDEIAPEISEPLIVNNKIEDLHKAFSIADRYFITNELFDNNGNAFAMAIEAINQC
                     ASIEQANTILKELNIKYQWDTDSSAFNYFYSIIKQKFNHV"
     gene            5580..6887
                     /locus_tag="PKI65_06915"
     CDS             5580..6887
                     /locus_tag="PKI65_06915"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HOB78207.1"
                     /translation="MNNIFKYLILFTLSFTLYLLNAQDVEYARQVFKKLTSTEFAGRS
                     LADSGIYKAADFIENEFKNNGISVFSQLNSYKQCFSLPQSLIIENAKVKLDNKELILG
                     HDYLPLPTSKTLKGEFNIKYIAINKNSKNKKILKAISKDPTDFYFLDFTDAYLLKAKD
                     IEEYNTLKSLYYSTKSIPLGYIVLDSSSFFYTAKSFFNINYPVIMLNTNTYDNSLKPQ
                     KVYIDYKVKTVSNAELCNIVGYIPGIKYPDSFIVIGAHYDHLGKIADKHFPGANDNAS
                     GTVMLLDLAKKLATEDEKPNYSIIFISFAGEEIGLLGSKYFTTNPPVDLKNIKLMINL
                     DMVGTGKDGISVVNAKKNPSIYNKLKLINEEKNYVVDMIEGGASCSSDHCPFDQRGVP
                     AIFIFSRDKDYRYYHVMEDDYEILPLDGYEGIFKLIYDLIMNY"
     gene            7228..8049
                     /locus_tag="PKI65_06920"
     CDS             7228..8049
                     /locus_tag="PKI65_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOB78208.1"
                     /translation="MKLLLERPIIFFDLEATGLDLVNDKILEIFMLKVNPDGSTDTYE
                     QLFNPEIPIPPEVTQITGITEEMVRNKPTFREKAQEIADFIDNADIAGFNSNKFDIPL
                     LAEELLNADVDIDFTNRNAIDVMVLFHRMEPRNLTAAYKYYCNKELQNAHTAKADTIA
                     TYEILLGMLERYIDDDNIVVNNEQNIKFENDVKKLSQLSKYYKFADLAGHILYDENDK
                     EVFNFGKYKGKVVEEVFKIEPQYYDWIMKAQFPRYTKKIVDNIWKKIKDTNIESE"
     gene            8175..10013
                     /locus_tag="PKI65_06925"
     CDS             8175..10013
                     /locus_tag="PKI65_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOB78209.1"
                     /translation="MNAKYTEDQIISLSWNEHVRMRPGMYIGKVGNGSSRDDGIYVLL
                     KETIDNSIDEFLMVKYPKIEIQIENNLVRVRDYGRGIPLGKVVDVVSKINTGAKYNSV
                     AFKKTVGLNGVGAKAVNALSQYYRIVSYRDNKAVEAVFSKGILQNTQKILTNEANGTL
                     VEFIPDEEIFGSYEFHDEFIEDMLWNYVCLNKRLKIIYNNKEFYSERGIVDLLHKKIS
                     GEILFDIIHIEEEDFEFAFTYVLGDTNEELFSYVNGQYTVQGGTHVQAFREAFVETIR
                     NFYNKNYDTYDIRNGLLVALSIRIHEPVFESQTKTKLGSMYMEPNGKSIKAYISEVIR
                     KYLDNELHRRADLAKIIQEKILFNEKQRKDINSIKKVSREVLKKASLHNKKLRDCKIH
                     FNTNHERRLETSIFITEGDSASGSITKARDANIQAVFSLKGKPLNVHKASKKAIFANE
                     EFALLIAALNIDNGLDNLRYNNVIIATDADEDGKHIRLLLLTFFLKFYPDLIKAGHLY
                     VLQTPLFRVRNKQKTIYCYNENEKQKALSQLGNNSEITRFKGLGEISPDEFKQLIGKN
                     IRLEPVVYNSEIKIDQLLDFYMGENTATRAEFVIENLRYNEILDLL"
     gene            10070..10369
                     /locus_tag="PKI65_06930"
     CDS             10070..10369
                     /locus_tag="PKI65_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78210.1"
                     /translation="MKKIIFIFAISLLFILQSCEKDCDGKGYLKLTNTSVHTVQKIMI
                     DGVNYGSLDPGESQTITLPAGEHEFQLVGISGGTGCSPAKVIIIECESQGFECSG"
BASE COUNT         3958 a         1339 c         1515 g         3673 t
ORIGIN      
        1 tttggtaaaa ttaacaatta attttatatt aatccaatat taaattaaac aattgcaatt
       61 tttactattt cctcaccctt ttatttttat acatcctttt aataatctag aataatcatt
      121 tgattctgaa cattatcttt ttttataaaa cgttctacta tttatactta gatatctttt
      181 tctttttttt tataaagaat gtgttatact tatattggta ctatattttg agtagttcta
      241 tatgtttttt gacgttttat acagacctct ttgtaaatgt aatgataata gattgaaagc
      301 aaatgttttg ttgaaatatt ttatcgctta attgtttcga ttacaccatc tgttttggtt
      361 aataacaaat atgaattatt ccaatagaga gcatctatag actctatatg tgagagagta
      421 tctaatattt tcattttagc ggataaaatg gtaaaagtat tatcatgatc ttttatgatc
      481 aaaggagaag tttttttaat ccaagataaa ttattatctt ttgcgataat attaccttga
      541 tatttaaaat ctcttactaa tttgccatta tccttatcta tccatgcgat gttatattta
      601 tcccacatca caagtgtatc gcctataaga gtgaaattat taaatcctgt gggtttacct
      661 actaatgacc cttgtttagc ttctctgagt tcatatattt taccttggat atctattaaa
      721 tatagtattt catttttttg ggaaaaatgt aaatctttta gaggagtatt aaatttataa
      781 tgagcttgct tatcgcctct tctattataa agagtaagat tattattatc tgctacgcaa
      841 atgtattctt tgttattcca cttagcgtaa gcaactctgc tattattgct agtttttata
      901 agtttccatc cctcgggagt attaccatca ctattgagat tatgtaatat gccatcgttg
      961 cttagataaa ataaacgttg cagtccatcc tcatatttga aaagagcata acccaatata
     1021 tttgcttgtt ttttccactt taggttacca gccatatcat acataaacaa tccactagga
     1081 gatacaagta atagatgctc ttttccatca tccattacta aagcatctat tatgtttttg
     1141 tctggcaagt ctatctctac aaaactttta gttatcaatc cttcaataaa aatttttttg
     1201 tcctgtatat aagtaatata aacattagat ggtgaatata taagtttggg atctttacac
     1261 tgtttgcata ttttattata taaaatgatc ttattattac tttgactatt tttaattttt
     1321 tcaatccaaa taaatatctt ttgggtagtg aggtcattat tatttatcat ccatagctgt
     1381 ccttgaaggt ctttattttt ataaaaggaa aatggtggag aaaaacaatt aaagaaagcc
     1441 aattcattaa tctcagacct tagatacgca gatatttttt tataaaattc tttttgagta
     1501 ataggaaagt atgatttatt taataattct aataatttta tttcttcaaa ataaaaaagc
     1561 aaagtatctt tgataaataa gtatgataat gaatgggagc tttttttagt attattaaaa
     1621 atattctcag tacacatcca agtatttttt gaaatcaatt ctgatttatt atttaattga
     1681 ttgataatat tagtagtatc tttacattgc attattagca aagggttttg atcaattata
     1741 ccaactaaaa catgtggttt tatcatattg gagaaatcta cattatcaaa aaatatatga
     1801 ttattaagcg acttaataat ataaggttgc aaagcattac tcaatggttg atcagaaatt
     1861 ttcgaaataa caaaaaattg ctccatatta gcaggtatta tggacaaatc aaaatcatac
     1921 tcagagctac ttatcagatg ttctttagga aatttttgat aaaagatcca taaatcatct
     1981 tgattattaa tatcaaaata ataaatttgt tcgatatcag ttaaaaattt tataccccat
     2041 tgagcccatt ttttatttat aaactttaat ttagaagtat ttaaagccaa atggaggtta
     2101 tagttagaat ttaaagtaaa atattcatct gaatatttat catagccttc gcctataggc
     2161 aaagattttt catctttagc ataaagaaca taaggaaaat atttcaaata taatatttca
     2221 tcatttagat tttggtaata tttatttttg atttttttaa aattcaattg ctcaaaccat
     2281 tttatgattt tatttttttt ataatcaata tttgcatata agatccaata aattttatca
     2341 ttatcattta ttatatatat ttttttaaaa taattttgcc atgacgacga atttttaaaa
     2401 aaatcaggaa aaacatttaa atataaagaa gtatcactat tataaaactc gtatgcagat
     2461 ttgtctatta gccatatttc atcaaaagtg ctatctatta tatcagatac attgtactta
     2521 tgtatcatca gattaggaaa attatatata gctaatccta ttaacaaaac aatgataacg
     2581 gagattatta tttgccaata tttattcatt atttattgta ctcaataaaa tttaatgcca
     2641 aattacaaaa aatatttgag tttaaggttt tttttattat tagtcatttc atttaatttt
     2701 ttggtttttt aaacatttac ctgtattttt gcaaattaaa taatcaaaaa aattcattta
     2761 caattatatg gatagaagtc aagtaatagg ttttatactt ctttttgcgc ttttaatagg
     2821 atatatgttt tacttatcac cttctaaaga agagctcgaa aggcaaaaac agatccaaga
     2881 cagtctaagg cttgtagagc agcaaaaaaa agattctatt tttttagctg aacaggctat
     2941 ccaagaatct gcaaaagata ctattaaaac tgaaagtgat atagatacaa cctatatagt
     3001 tgaaagccaa aaaagtatac cagaagctta tacttttttt aaacaatctt atcaaacaga
     3061 agatagcata atatttattg aaaatgatgt agcaattatt tcattttatt gtaagggtgc
     3121 catattacat agtgttaaat taaaaaattt caaagattat caaaataatc ctgtccaact
     3181 aatagatact aataattatg aattttcttt tgtttttaga gcattcgata gatttttaga
     3241 aactaaaaac ttatttttcg aaccatccat taaaaatagc aatggcaata tttatgtaac
     3301 atttatagca aacgcacgtg atacattaac aaatgaaata ttaggtgggg taaagataga
     3361 atattcaata ccacaaaatg aatatatggt agactataaa ttatctttta taaatcctga
     3421 tgaaattgga gttttacctt tagatatttt gactttttct tgggcaaaca atatgataca
     3481 gcaggaagaa gacaaaaagg tagagactat taatactacc atctgctaca tgaatagtga
     3541 aaaaaaagta aaacacttaa aagcaaaaga tcaaaaacaa acattaaatt ttagagttaa
     3601 atggattggg ttaaaacaac aattttttac ccatacgctg ataaatgttg aagattattt
     3661 cagctcagcc gagttagaga ccaagtcatt tagtcccgat gatacttcta gatatttgag
     3721 acgaatgtca gtaggtatga caatagagca tagcactgat agaccagtac atttcaaaat
     3781 ctatactgga cctcttaaat atagtatatt aagaagttat aaattgagat tagaaaggga
     3841 aatacctctc ggttggggtt ttgcccccat agcatgggtt aatagatttt tagtgattcc
     3901 cgtttttaat tggttggaac gctacggttt aaattatgga ataataatat taatattgac
     3961 ccttttatta aaaatagtgc ttctaccttt tacttataaa acatacatga gtactgctaa
     4021 aatgcgagca ttaaaacctg aacttgacaa aataaatcaa aaatattcta aacccgatga
     4081 tgctctgaaa aagcagcaag caacaatgga gctatataaa aagtctaaaa taaatccagc
     4141 aggtggttgc tggcctatgt tgctgcaatt ccctatatta atagctatgt ttaggttttt
     4201 cccatcagct attgagctta gacataagcc atttctctgg gctcacgatt tatcagcatc
     4261 agattctatc ttagatctag ggtttaatat ccccttatat ggcgatcatg tgagtttatt
     4321 tgctttattg atggctatat ctacactttt gtatacttac attaataata aactgacacc
     4381 tactgatact aatgctccag gaatgaaatt tatgatgtat gctatgccta ttatattctt
     4441 aggtatcttt aataattact catcagcact tagttattat tatttcttat ttaatgtggt
     4501 ttctatattg cagactctta cttttcgtta ttttgtaaat gaagaaaaaa taagagctaa
     4561 attattagaa aattggaaaa aaccagtcaa aaagtcatcc tggcaacaac ggatggaaga
     4621 gctagcaaag cagcaacaaa ctaatgtaaa gaaaagataa atattttttt catatttctt
     4681 gtattgatta ctttattttt acattatttt ctaaaatttg gttcttttaa tttactaact
     4741 taattttgca taaatttaat tttattagat atgataaata aaatgaaaat tgatttacac
     4801 aaaaaaatgt tagattatta tttagatcta atgataaaag atgagaatat ttctactatt
     4861 aagctattat tagaagaaat agaatcatta cactctgtag ttttgaaaga atatattaat
     4921 ttattaccat tagataatat tgcagacaga attatagata tagatgacga attaataata
     4981 gatactgatt tagatgatga tgctaaaaca attgattcaa ttattaacga ttttgatgat
     5041 attgagataa aaattgaaga agaactagaa atagaacatc atattactga atctgaaaaa
     5101 atagatgaat taaatcttga aaatgaacaa atagatgtag aatttaatca agacaaaatt
     5161 gatttaaaag aagaaattat tgattatgaa tcaattagcg aagaaagcga acaatatctg
     5221 tctaatgata taaaaataaa tataaaaact gaacagacag aacaaaaaac catatttgaa
     5281 tcatttataa atggtagtga tgaaatagct cccgaaatct ctgaacctct gatagttaat
     5341 aataagatag aagatttaca taaagctttt agcatagcag atagatactt cataacaaat
     5401 gagctatttg acaataatgg gaatgccttt gctatggcaa tagaagctat aaatcaatgt
     5461 gcaagcatag agcaagcaaa tacaattttg aaagaactaa atattaaata tcagtgggat
     5521 acagattcta gtgcttttaa ttatttttat tctattatta aacaaaaatt taatcacgta
     5581 tgaataatat ctttaaatat cttattctat ttactctatc atttactctc tatctattaa
     5641 atgctcaaga tgtagagtat gctcgacagg tttttaaaaa actaactagc acagagtttg
     5701 caggacgttc tttagcagat agtggaatat ataaagctgc agattttata gaaaatgaat
     5761 ttaaaaataa tggaatatcc gttttttcac agttaaattc ttacaaacaa tgtttctcat
     5821 taccacaatc attaataata gaaaatgcta aagtgaagtt agataataaa gaattaatat
     5881 tggggcacga ttatctgcct ttgcccacta gtaaaacttt gaaaggtgaa ttcaacataa
     5941 aatatattgc gataaataaa aattctaaaa ataaaaaaat tcttaaagct atttctaaag
     6001 accccactga tttttatttt ttagatttta cagatgcata tttattaaaa gcaaaggata
     6061 tagaagaata taatacatta aaatcattat attactcaac caaatctata cctttaggat
     6121 atatagtatt ggatagttca tcattttttt acactgctaa atcttttttt aatattaatt
     6181 atcctgtcat aatgttaaat accaatacat atgacaattc acttaagcca caaaaagtat
     6241 atattgatta taaagtaaaa actgtatcta atgcagaatt atgcaatatt gtgggttata
     6301 taccaggtat taaatatcca gatagtttca tagtgatagg agcacattat gatcatttag
     6361 gcaaaatagc agataagcat tttccaggag ctaatgacaa tgctagtggt acagttatgc
     6421 ttttagatct agccaaaaaa cttgctactg aagatgaaaa gcccaattat tcaattattt
     6481 ttataagctt tgcaggagaa gaaataggac ttttaggatc taagtatttt actactaatc
     6541 caccagtaga tttaaaaaat ataaaattga tgatcaattt agatatggta ggtacgggca
     6601 aagatggtat ctctgtagtg aatgctaaaa aaaatccatc tatttataat aaattaaaac
     6661 taataaatga agaaaaaaat tatgtagtag atatgataga gggaggtgca tcttgtagtt
     6721 ccgatcattg cccttttgat cagcgtggtg tgcctgctat ttttatattt tcgcgagaca
     6781 aagattatcg ttattatcat gtaatggaag atgactatga aattttacct ttagatggtt
     6841 atgaaggtat ctttaagctt atttatgatt tgattatgaa ttattgataa ttataattca
     6901 aaaatttcga accattacaa agtttatatt tttaatatgc gaaaaaaaat agtaatttta
     6961 caaaaatgaa tttgttaata ttagtttatt aattataggg gtagttaaat attaaccaca
     7021 aagttctcaa agaatgcaca aagaacacag agaaggatgg aattaaaagg gctacaaatg
     7081 ggactttgtg gactttgtgt aataacttgg tgccctttgt ggtaaaaaaa tcaacctaaa
     7141 taatttatcc acaataaacg ttatagaacc aaaataattt tttgtaattt ttcatttatc
     7201 tttgcttatc aaaaaaaaaa ttataatatg aaacttttat tagaacgtcc cattattttc
     7261 tttgatttag aagctacagg cttagatcta gttaatgata aaattttaga aattttcatg
     7321 ctaaaggtaa atccagatgg tagtactgat acatacgaac aactttttaa tccagaaata
     7381 cctatacccc cagaagtaac tcaaatcact ggtatcaccg aagagatggt tagaaataaa
     7441 cccactttta gagaaaaagc tcaagagatt gccgatttta tcgataatgc agatatcgct
     7501 ggatttaatt ccaataaatt tgatatacct ttattagctg aggaactatt aaatgctgac
     7561 gtagatatag attttactaa tcgtaatgct atagatgtaa tggtactttt tcatcgcatg
     7621 gaaccccgca atctcactgc tgcatataaa tattactgta ataaagaact ccaaaatgcc
     7681 catactgcta aagctgacac cattgctact tatgaaatct tattaggtat gctagagaga
     7741 tatatcgatg acgataatat tgtggtaaat aatgaacaaa atataaaatt cgaaaatgat
     7801 gtcaaaaaat taagtcaatt atctaaatat tataaatttg ctgatttagc tggtcatatt
     7861 ttgtatgatg aaaacgataa agaagttttt aattttggta aatacaaagg aaaggtagta
     7921 gaagaggttt ttaagataga acctcaatat tatgattgga taatgaaagc ccaattccct
     7981 cgatatacta aaaaaatcgt agataatatt tggaaaaaaa tcaaagatac taacattgaa
     8041 tctgaataaa atttaaaata gacataatat agagaatcaa aagtttttct tctaatagta
     8101 gtaatgtggt atttcaaaat tcctactttt tttgtaattt tgtataaata aattgatgat
     8161 aaaatctttt atacatgaat gctaagtata cagaagacca aataatatca ctatcgtgga
     8221 atgaacacgt acgcatgaga ccaggtatgt acattggcaa agtaggcaac ggaagctcta
     8281 gagatgatgg tatatacgtt ttattaaaag aaacaataga caactccatt gatgaatttt
     8341 taatggtaaa atatcccaag atagagatcc aaatagaaaa taatctagtg cgagtgagag
     8401 attatggtag aggcatacca ttaggcaaag tagtagatgt agtttctaaa ataaatactg
     8461 gtgccaaata taatagtgta gctttcaaaa aaactgtcgg tttgaatgga gtaggagcta
     8521 aagctgtaaa tgcattatca caatattata gaatagtttc ttatagagat aataaagcag
     8581 tagaagctgt tttttcgaag ggtattctac aaaatactca aaaaatatta actaatgaag
     8641 ctaatggtac actggtagaa tttattcctg atgaagaaat ttttggatct tatgagtttc
     8701 atgatgagtt tatagaagac atgctatgga attatgtctg cctcaataaa cgattaaaaa
     8761 ttatctataa caacaaggaa ttttatagtg aacgtggtat agtcgatcta ttacataaga
     8821 aaatttcagg tgaaatctta tttgatatca tacatataga agaagaagat tttgaatttg
     8881 cctttactta tgtgttaggc gatactaatg aagagttatt ttcatacgta aatggacaat
     8941 atacagtgca aggtggcacc cacgtacagg cctttagaga agcttttgta gaaactataa
     9001 gaaattttta taataaaaat tatgatacat atgatattcg taatggcctt ttagtagcat
     9061 tatctatcag aattcatgaa ccagtcttcg aatcccaaac caaaactaaa ttaggttcta
     9121 tgtatatgga acctaatggc aaatctatca aagcatatat aagtgaagtg atacgaaagt
     9181 atttagataa tgaattgcat cgtagagctg atcttgctaa aatcattcaa gaaaaaatac
     9241 ttttcaatga aaagcaaaga aaagatataa atagtatcaa aaaagtatct cgtgaagttt
     9301 tgaaaaaagc tagtttgcac aataaaaaat tgcgagattg caaaatacat ttcaatacta
     9361 atcatgaacg aagattagaa acttcgatct ttataactga gggcgactca gctagtggca
     9421 gtattacaaa agctagagat gccaatattc aagctgtttt tagtctaaaa ggcaaacctc
     9481 taaatgtgca taaagcaagt aaaaaagcta tttttgctaa tgaagaattt gcccttttga
     9541 tagcagctct taatattgat aatgggcttg acaatttaag atataataat gtgataatag
     9601 ctactgatgc agatgaggat ggcaaacata tcagattgct tcttcttaca tttttcttaa
     9661 agttttatcc agatttaata aaagctggac atctatatgt attacaaaca ccactattta
     9721 gagtgagaaa caaacaaaaa actatttatt gttataatga aaatgaaaag caaaaagctc
     9781 ttagtcaatt aggtaataat agtgaaatta caagatttaa aggcttaggc gaaatatctc
     9841 ccgacgaatt caagcaattg atcggtaaaa atatacgatt agaaccagtt gtttataatt
     9901 ctgaaattaa aatagatcag cttctagact tttatatggg cgaaaataca gctactcgag
     9961 ccgagtttgt catagaaaac cttagatata acgaaatttt agatttatta tagatattta
    10021 tgcgataaat cattttattt tattaatttt gtataaaatt attttttata tgaaaaaaat
    10081 tatattcatt tttgcaatat ctttactttt tattttacaa tcttgtgaaa aagattgtga
    10141 tggtaaagga tatttgaaat taacaaatac ttctgtccat actgtacaaa aaatcatgat
    10201 cgatggagta aattatggaa gtttagatcc cggtgaatca caaacaatta cactgccagc
    10261 aggtgaacat gaatttcaat tagtaggtat tagtggtgga acaggttgta gtcctgccaa
    10321 agtaataatc atcgaatgtg aaagtcaggg atttgaatgt tcaggataat tttttcgttg
    10381 atattgaaat aactgatttt tagatgatta attaaaataa aaaaaccgta attgtagcgg
    10441 caactcacga atcattagtt cataaaattt taattttaaa atagt
//
LOCUS       DAOPRN010000063        10430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_5380_length_10430_cov_144.211277, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000063 DAOPRN010000000
VERSION     DAOPRN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5380_length_10430_cov_144.211277"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1915
                     /locus_tag="PKI65_06935"
     CDS             245..1915
                     /locus_tag="PKI65_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HOB78211.1"
                     /translation="MSFINQLLTGTSISHSIFILAIIVAIGLALGKIKIFGISLGVTW
                     VLFIGIFLGHFGFTVDEKILNFVKDFGLILFVYFIGLQVGPSFFASLKKSGLKLNLLA
                     LTIVILGVIVTYILHLITGLPISTMTGILSGAVTNTPSMGVAQQTFIDMTGRADPSIA
                     LGYAVCYPLGILGVIFVIILLRLIFKIDLEKEKKDLEKLEKSQISQARVFSIQITNPD
                     IFGKNIFDIKKLINKDFVISRVYHSDINTIEIAQPKTILNKDDKILVVTNLQNIDEIN
                     YLLGKQIEMDAEQWNKLDQQLISEQIIISNPKINGKTIGQLKLRSNFGVNVTRVNRAG
                     FDLVASPNLNLQMGDRLTVVGTESAIINIEKFLGNSLKQLNEPNLIAIFIGIALGVLL
                     GSIPLRFPGIPQPIKLGLAGGALIVAILLSNFGAKYHLVTYTTVSANLMIREIGISMF
                     LASVGISVGKDFVQTIINGGYLWIAYGFIITIVPILIVGIIGRLVFKLNYFTLIGLIT
                     GSVTNPPALAFANSLSDNDAPVVSYSTVYPLTMFMRVITAQILILFFV"
     gene            complement(1901..3232)
                     /gene="tilS"
                     /locus_tag="PKI65_06940"
     CDS             complement(1901..3232)
                     /gene="tilS"
                     /locus_tag="PKI65_06940"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOB78212.1"
                     /translation="MVKLEKEVYNFLNKNIENFKDIKYLLGVSGGIDSMVLAYIFQRL
                     QLKFGVAHINFQLRGDDSDSDEIFVKQWFDPFDIPLYFKKVNTIEYAEKNKVSIEEAA
                     RQLRYDFFEKLAYKYDYNYIVLAHHANDVIETLFINLLRGATINGLASIPKMRDNIIR
                     PLWHVSRDEIERYTKYHNIPYRLDKTNDELIFLRNRIRHELLPLLRDMRAGVDKTLIN
                     NIDVLQAQSQCYNYLMKNYINSKINKYSDHSFSLDMSNIHTISLAVLHFLLSPLSATY
                     DQLQNIINALPLSETRKFILPDYDVYTQKNILYVINNKTIFPELIIDKETKEIKTLIK
                     LKIQILPIQQIKIKKSNNYAYIDLNKIIFPLKLRKIRDGDMFIPLGFHGKMKISDFLI
                     NQKASPIDKHFQYVIEDASSKIIWVVGRRISDLVKIDKNTKQVMVIELYKK"
     gene            complement(3226..6108)
                     /locus_tag="PKI65_06945"
     CDS             complement(3226..6108)
                     /locus_tag="PKI65_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78213.1"
                     /translation="MAKTFKNLEEYIELTKQVVIANRNKGNRYSEILSSLYADPVHFL
                     DEILQNAEDACARIETLGYIKINFDVNGIAIYHNGDPFNEADLIAITTYGKTTKKQLS
                     NINKIGKFGIGFRSVFGITDRPEIHSGEYHFAITDFELLEHIEPKAIPVGYTTYFWFP
                     FKKNISSRVLWDDVRLWDHRFILFLNYIYKVELVNQPNNENYFIEKIITDDDKIIVIK
                     SNLPECLSEENYLLISKNNNKQKRLSIAMSLDKNETRNPLFVFFPTKETLNLPFLVHG
                     FFTTTPTREQIPWDNKAVKENQILLKQLNNLFKNFLKELRKRALLTPKLYEAFTLTNH
                     THPIEKLWTEQWIDFLKIDRSILTKEKKYLPPALTALPKHKSLVELIPANALNKLWNK
                     KAWIEPSILDYPNITELIKKELYIRIIDERDFGYAVKELNLNKRYKLKQIELFYSLLA
                     EHPELVLPINKDKFYSLTDFAWLYTEDKKWLCHNDQLSNIYLSGAPDPSQNINPIFLK
                     QEKIVNFLYSLQIKPYEIADGVNNQLTALMQKKAYRKLWKLMLENIDNIDFELIANFS
                     CFPNLKKEWKKADEVYLPTKELEYYFSYHVDFIDCKWFERNFKLPFSQLQYITKKIGV
                     CKFPKKIKIDKKLTEEEKISLRRNTEVKPIIEEKIIDWNIEGLDEFIENINFEKSNFL
                     FHWILQFPDDYWWGQYTWASYIREDRAIFRSMAWHTLYKNAWIYDDNNHLMPTAEIDL
                     SKIKSEYNLSANEIKNFINLFELTHYDDFGLSLDEYKLIQLYRDGTLGIEGMLNGSFA
                     YPKKNIHYFNLKELKNKGIITNSLKILEEFTPDFPINIFPFVQIKKPFEKMFSNLLDI
                     AYEWIMQQILANNENYKIKKSNEGYIDLLKSNFVMKRYYVGGREYSNDYFMIYKSVLE
                     PNINIPASLILIDLSDNENILLIEIEDYEQFIGNNMINPEIIFLLW"
     gene            6307..6588
                     /locus_tag="PKI65_06950"
     CDS             6307..6588
                     /locus_tag="PKI65_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78214.1"
                     /translation="MKKLRILTIALLLAISFVGCKDDDKESNCDKEIQDIIDQYGQPT
                     MQQTISTGDQNAIQYMYEDDGIAFTFYWGKGYKGCEVVRQSWSFGDLDF"
     gene            complement(6846..8093)
                     /locus_tag="PKI65_06955"
     CDS             complement(6846..8093)
                     /locus_tag="PKI65_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOB78215.1"
                     /translation="MLRLRLIIMNFFQFFIWGSWLLTIGAFWFQYKGWSPTEFGLVFS
                     TMGIASLFMPTIAGIIADKWINTEKLYALFHLCGAILLFILPSINNPITFFWVMLINM
                     CFYMPTISLAITICYTSIENAKMDLVKTYPPIRVFGTIGFIVALWTVSLLKVETSPVQ
                     FYIASGASLFLALYALTLPKCPPLGKGKKANLSEALGLDAFKLFKDTKMALFFIFSFL
                     LGVSLQLTNAYGDTFIHDFAKIPEYANSLAVKYPAIIMSISQISEALFILAIPFFLKR
                     YGIKNVMLISMIAWVLRFGLFAYGNPTDRLWMIILSCIIYGVAFDFFNISGSLFVETQ
                     VSPSIRASSQGLFMLMTNGLGSLTGSLLSGLVIEKFFVVDGNIMWHQTWLTFAIYSLI
                     VAIAFAIFFKHKHNPADFENVHV"
     gene            complement(8103..8747)
                     /locus_tag="PKI65_06960"
     CDS             complement(8103..8747)
                     /locus_tag="PKI65_06960"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOB78216.1"
                     /translation="MAKIELEVSGLMPSSPSINAYAIILIEKYGNRTLPMLIGVAEAQ
                     SIAIAIEKISYPRPLTHDLFVQLIKEIDIRIVEVLIYKYAEGIFYAYLVIEYNGKIKR
                     IDARPSDCIAIALRANAPIFVYEYILDEVGNIFEDKYEINKNISEDDEGNAYDISQDL
                     SLEKLKDMDPQFIKTRLEILSIEDLQLLLDKCIEGELYELASLIRDEISRREQK"
     gene            complement(9307..10317)
                     /locus_tag="PKI65_06965"
     CDS             complement(9307..10317)
                     /locus_tag="PKI65_06965"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78217.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGIQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFKKFESKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEIVTSHRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNKTYVNLN
                     IC"
BASE COUNT         3613 a         1541 c         1359 g         3917 t
ORIGIN      
        1 cataattttt tattttttgc aaatttaata aattttctga tattaatcct gtatagattt
       61 taacattttt aatcaatcga tacattaatt atttatttta atctattcgt aaattcaaca
      121 ttactactct tatttatttt cataaatttt ttcttttact aaaataatat tacctttgta
      181 gtcagtttaa taatcaaaaa caataaccat ttaaaataca atagtttata tttgttttgt
      241 tattatgtca tttattaatc aattacttac aggcaccagt atctctcatt cgatttttat
      301 acttgctatc atcgtagcta ttggacttgc attaggtaaa ataaaaatct ttggtatttc
      361 attgggtgtt acatgggttt tatttattgg aatttttttg ggacattttg gttttactgt
      421 tgatgaaaag attttaaatt ttgtaaaaga ttttgggtta attttatttg tttattttat
      481 aggattacaa gtaggtccta gctttttcgc ttctctcaaa aaaagtggac tcaaattaaa
      541 tcttttagct ctaactattg tcattttagg tgttattgta acttatatcc tgcatttgat
      601 cacgggctta cctatttcta ctatgacagg catattatct ggagcagtaa ctaacacccc
      661 tagtatgggt gtagctcaac aaacttttat tgatatgaca ggcagagccg acccttcaat
      721 tgcattaggt tatgctgttt gttatccatt aggtatttta ggtgttattt ttgttattat
      781 tttattacgt ttgattttta aaatagattt agaaaaagaa aaaaaagatt tagaaaaatt
      841 agaaaaatct cagatcagcc aagcacgcgt attctctata caaattacaa atccagatat
      901 ttttggcaaa aatatttttg atattaaaaa attgataaat aaagattttg taatttctcg
      961 tgtttatcat tcagatatta atacaataga aatcgcacaa cctaaaacta ttttaaataa
     1021 agatgataag atattagttg taactaattt gcaaaatata gatgaaataa attatttatt
     1081 aggtaaacaa atagaaatgg atgcagagca atggaataaa ttagatcaac aattaatttc
     1141 agagcaaata attatctcaa atcctaaaat taacggtaaa actatcggac aattaaaatt
     1201 gagaagtaat tttggtgtta atgttactcg tgttaatcgt gctggctttg atttagtcgc
     1261 tagtccaaat ctcaaccttc aaatgggtga tagattgact gtagtaggta ctgaatcagc
     1321 tatcattaat attgaaaaat ttcttggcaa ttcacttaaa caattgaatg aacctaatct
     1381 aattgctatt tttattggta tcgcattagg agtattatta ggaagtatac ctttaagatt
     1441 cccgggtata ccgcaaccta taaagcttgg attagctgga ggagctctaa ttgttgcaat
     1501 attattaagt aattttggag ctaaatacca cttggttaca tacactacag tgagtgctaa
     1561 tttgatgatt agagaaattg gtatttcaat gtttttagcc tcagtaggca ttagtgttgg
     1621 caaagatttt gtacagacta taataaatgg tggttatctc tggatagctt acggatttat
     1681 tattactatt gtacctattc tcattgtcgg tattattggt agattagtat ttaagctaaa
     1741 ctattttact ctcattggac tcattactgg tagtgttaca aatccaccag ccttagcttt
     1801 tgccaattca ttgtcagaca atgatgcacc tgtcgttagc tattctactg tttatccact
     1861 cactatgttt atgcgagtga ttacagccca aattttaata ttattttttg tataactcta
     1921 ttaccattac ttgtttagta tttttatcta tttttactag atctgatatt ctacgaccta
     1981 ctacccatat gattttacta ctggcatctt ctattacata ttgaaagtgt ttatcgatgg
     2041 gactagcttt ttgattaatt agaaaatcac ttattttcat tttaccatga aatcctaagg
     2101 gaataaacat atctccatct cttatctttc gcaatttcaa aggaaaaata atcttgttta
     2161 gatctatata agcataatta tttgactttt taatttttat ttgttgaata ggtaatattt
     2221 gtatttttaa tttaattagt gtctttattt ctttagtttc tttatcaata attaattcag
     2281 gaaaaatagt cttattattt atcacatata agatgttttt ttgagtataa acatcataat
     2341 ctggtaaaat aaattttcta gtttcactga gaggtaatgc atttattata ttttgcaatt
     2401 gatcgtaagt agctgataat ggtgagagta aaaaatgtaa aactgccaat gaaatcgtat
     2461 gaatattaga catatcaagc gaaaaactat gatctgaata tttattaatt ttagaattaa
     2521 tataattttt catcaaataa ttgtaacatt ggctctgcgc ttgtaaaaca tctatattat
     2581 ttatcaaagt tttatctaca ccagctctca tatctctaag caatggtagt agttcatgac
     2641 gaatgcgatt tctcaaaaat atcaattcat catttgtttt gtctaatcta tatggaatat
     2701 tgtgatattt ggtatatctc tctatttcat cacgagatac atgccataat ggacgtatga
     2761 tattatctct catcttgggt atactagcta atccattaat agtagcacca cgtaataaat
     2821 ttataaacaa agtttctatt acatcattag catgatgtgc caatactatg tagttataat
     2881 catacttata tgctaatttc tcaaaaaaat cgtatctcaa ttgtctagca gcctcttcaa
     2941 tagatacttt atttttttca gcatattcaa tcgtatttac ttttttaaaa tataaaggaa
     3001 tatcaaatgg gtcaaaccat tgttttacaa atatttcatc actatcacta tcatcgcctc
     3061 ttaactgaaa atttatatga gctacaccaa attttaattg caaccgttga aatatgtatg
     3121 ctaaaaccat actatctata ccaccactca ctccaagcag atatttaata tctttaaaat
     3181 tttcaatatt tttatttaaa aaattataaa cttctttttc taattttacc ataataaaaa
     3241 aattatttct ggatttatca tgttattacc aataaattgc tcgtaatctt ctatttctat
     3301 taataaaata ttttcattgt cagaaaggtc tatcaatatc aaactagctg gtatattaat
     3361 attaggctct aacacagatt tgtaaatcat aaaataatca tttgagtact ccctaccacc
     3421 cacataatac cttttcatta caaagttgct ctttagcaga tctatatatc cttcattaga
     3481 ttttttaatt ttataatttt cattattagc taatatctgt tgcattatcc attcataagc
     3541 tatgtctaaa agattagaaa acatcttttc aaaaggtttt ttaatctgta caaaaggaaa
     3601 aatattaatt ggaaaatcag gggtgaattc ttctaatatt tttaatgaat tagtaattat
     3661 tcctttgttt tttaattctt ttaaattgaa ataatgaata ttttttttgg ggtatgcaaa
     3721 actaccattt agcatacctt caattccaag cgtaccatct cgatataact gtatcaactt
     3781 gtactcatct aatgagagac caaaatcgtc ataatgagta agttcaaaca aatttataaa
     3841 attctttatt tcattagcag aaagattata ctctgatttt atttttgata aatctatttc
     3901 agcagtaggc atcaaatgat tattgtcatc atagatccaa gcatttttat ataaagtgtg
     3961 ccaagccata gaacgaaata tagctctatc ctctctgatg tatgatgccc atgtatattg
     4021 gccccaccag taatcatcag gaaattgcaa aatccaatga aaaagaaaat tgcttttttc
     4081 gaaatttata ttttcgataa actcatccaa gccttctata ttccaatcta ttatcttttc
     4141 ttctattatt ggtttcactt cggtatttcg gcgcaatgat attttttctt cttcagtcag
     4201 ttttttatct atttttattt tttttggaaa tttgcaaaca cctatttttt ttgttatata
     4261 ttgtaattgg gaaaatggta atttgaaatt acgttcaaac catttacaat caataaaatc
     4321 aacatgataa gaaaaataat attctaactc tttagttggt aaatatacct catcagcttt
     4381 tttccattct ttttttaaat ttggaaaaca gctaaaatta gctatgagtt cgaaatctat
     4441 attatcgata ttttcaagca ttaatttcca taatttgcga taagcttttt tttgcattag
     4501 agctgtcaat tgattattca cgccatctgc tatttcataa ggttttattt gtaaagaata
     4561 taaaaaattg actatttttt cttgttttaa aaatattgga ttaatatttt gacttggatc
     4621 aggtgctccg ctcaagtaaa tattggataa ttggtcatta tgacacaacc attttttatc
     4681 ttcagtataa agccatgcaa aatcagtcaa actataaaat ttatctttat taataggtaa
     4741 aaccaattct ggatgctctg ctaataaact ataaaataac tctatttgtt ttaatttgta
     4801 tcttttattt aaattcaatt ctttgacagc ataaccaaag tctctttcgt caattatcct
     4861 aatatacaat tcttttttaa tcaattcagt gatattaggg taatctaaaa ttgaaggttc
     4921 tatccacgct tttttattcc ataatttatt taatgcatta gcaggaatta attctactag
     4981 cgatttatgt ttaggaagtg ctgtaagtgc tggtggtaaa tatttttttt ctttagttaa
     5041 aatagatcta tctattttta aaaaatctat ccattgttcg gtccataatt tttctatagg
     5101 atgtgtatgg ttagttaaag taaaagcttc atatagcttc ggtgtcagta atgctctttt
     5161 acgaagttct tttagaaaat ttttaaataa attatttagt tgttttagta aaatttgatt
     5221 ttctttaaca gctttattgt cccatggtat ttgttcgcga gtaggagtag tagtgaaaaa
     5281 accatgtact aaaaagggta aattcaaagt ttcttttgta gggaaaaaca caaataatgg
     5341 attgcgtgtt tcatttttat ccagagacat tgctatagat aatcttttct gcttattatt
     5401 atttttacta attaataaat aattctcttc tgataagcac tctggcaaat tactttttat
     5461 cacgattatt ttatcatcat cagtaattat tttttcgata aaataatttt cattatttgg
     5521 ctgatttact agttctactt tataaatata attaagaaat aaaataaatc tatgatccca
     5581 taaccttaca tcatcccata ggactcgtga tgatatattt tttttaaatg gaaaccagaa
     5641 ataagttgta taacccacag gtatagcctt aggctcgatg tgctcgagta attcaaaatc
     5701 tgtgatcgca aagtgatact cacctgaatg tatttctggt ctatctgtaa taccaaaaac
     5761 cgaacgaaaa ccaataccaa atttaccaat tttattaata ttagataatt gtttttttgt
     5821 agttttacca taggtggtaa tagctatcaa atctgcttca ttgaatggat cgccattatg
     5881 atagatagct ataccattta catcaaaatt aatttttatg taacccaatg tttctattct
     5941 ggcacaagca tcctcagcat tttgcaagat ttcatctaaa aaatgtactg gatccgcata
     6001 tagtgatgac aatatttcag aatatcgatt gcctttattt ctatttgcta tcactacttg
     6061 ttttgtaagt tctatatact cttctaagtt tttaaatgtt tttgccatga tttagtaata
     6121 aaatattaac aaaattatta aaaataaaaa taaaagtaaa atgactaata aaatactcta
     6181 aaaatttaaa ttttttagat caaaataatt aaataaatac ctaaaaatat ttatttaata
     6241 aaatcatatg catataatac aaaattttat ataattttgc aatgtaaaac tttaaaaaaa
     6301 attattatga aaaaattaag aattttgaca attgctttgt tattagcaat atcctttgta
     6361 gggtgtaaag atgatgataa ggaatcaaac tgtgataaag agattcaaga cattatagat
     6421 cagtatggac aacctactat gcaacaaaca attagcactg gagatcaaaa tgctatacaa
     6481 tatatgtatg aagatgatgg tatagctttt actttttatt ggggcaaagg atataaggga
     6541 tgtgaagttg ttcgacaatc ctggtctttt ggtgatttag atttttagag aaaatctaaa
     6601 aagcatatca aacaaaataa gggttagata tctaaccctt attttgttta taaaattcta
     6661 attatcaaat ccaaaatcaa tacgacaatt actcatttta agttattttg atagacgttt
     6721 aataaatctt gaaaaagtga caatgctaat ttaagtagat taatgataat caaaattcat
     6781 taatacaaaa atatctattt acaaaattta attatcaatt attattttta ggttagaaaa
     6841 agaaattaga cgtgaacatt ttcgaaatca gcgggattat gtttatgctt aaagaatata
     6901 gcaaatgcaa tagcaactat taaagaatag atagcaaaag tcaaccaagt ttgatgccac
     6961 attatattac catcaacgac aaagaatttt tctattacta aaccactcaa taagctacct
     7021 gtcaatgagc ccaatccatt agtcataagc ataaataaac cttgtgaact agcacgtata
     7081 gaaggggata cttgggtttc tacaaataat gatcctgaaa tattgaaaaa atcaaatgcc
     7141 acaccatata taatgcaaga taaaattatc atccataatc tatcagtagg gttaccatat
     7201 gcaaataatc caaatctaag cacccaagct atcatgctta taagcattac atttttaatt
     7261 ccataacgct ttaagaaaaa aggaatagct aaaataaata atgcttcaga tatttgagat
     7321 atagacataa tgatggctgg atattttaca gctaatgaat tagcatattc tggtatttta
     7381 gcaaaatcat gaataaaagt atcaccatac gcattagtta attgcaagga gactcctagt
     7441 aaaaatgaaa aaataaagaa aagtgccatt ttagtatctt taaatagttt gaatgcatct
     7501 aatccaagtg cttctgataa attagctttt ttacctttac caagtggtgg acatttgggc
     7561 aatgtgagag catataatgc taagaataaa gaagcacctg aagcaatata aaattgcaca
     7621 ggagaagttt ctacttttaa taagctaaca gtccataaag ctacgataaa gccaatagta
     7681 ccaaatactc taataggagg atatgttttt actaaatcca ttttggcatt ttcgatagat
     7741 gtatagcata tagtaatagc taatgaaata gtaggcatgt aaaaacacat attaatgagc
     7801 atcacccaaa aaaatgtaat aggattattg atactaggta aaataaaaag taaaatagcg
     7861 ccacataaat gaaataatgc ataaagcttt tctgtattaa tccatttatc tgcaataata
     7921 ccagctatag taggcataaa taatgatgct atgcccatag ttgaaaaaac taaaccaaat
     7981 tctgtaggag accatccttt gtattgaaac caaaatgctc ctattgtcaa aagccatgaa
     8041 ccccaaataa aaaattgaaa gaagttcatg ataattaatc tcaatcttaa catattatag
     8101 ttttattttt gttctcttct acttatctca tctcttatca acgatgccaa ctcatataat
     8161 tccccttcta tacatttatc taataataat tgtaaatctt caatagataa aatttctaat
     8221 ctagttttta taaattgggg atccatatct tttaattttt ctaatgaaag atcttgagat
     8281 atatcataag cattcccctc gtcatcttca cttatatttt tatttatttc atacttatct
     8341 tcgaatatat taccaacctc atctaatata tactcataaa caaaaatagg tgcattagct
     8401 ctgagagcta tagctataca gtctgaagga cgcgcatcta ttctttttat ttttccattg
     8461 tattctatta ctaagtatgc ataaaaaatt ccctcagcat atttatatat caatacttct
     8521 actattcgta tatcaatttc tttaattaat tgaacaaata aatcatgtgt taatggacga
     8581 ggatatgata ttttttcgat agctatagca atagattgtg cttctgctac acctattaac
     8641 attggtaaag tacgattacc atatttttct atcaatatga tagcataagc attaatagat
     8701 ggactactag gcattagtcc actaacttcc aactcaatct tagccattga aattttttaa
     8761 gttacaaaga taaggaaaaa aaatataata acgtaaaaat tataaaaata ctaatttata
     8821 tttttgatcc caataaaaaa tatgtataaa agaatgatat taggaataaa ttttttgaaa
     8881 tacttaaatt aaattatact aacaatgaag atgtaattag taaatacatt ttctaataaa
     8941 tactaaaatt ttttcttttc acgttctaac aaatttttat tgtgataaag agaaaagtaa
     9001 taaaagaaat taggttctat aacgttttgt atgggtaaag attaaaaaaa gagtaattct
     9061 acaagaaatt cgttaatatt ggttttttta ttgaacagtt aaatatcaac cacaaaattc
     9121 tctgagaatg cacaaagaac actgagaaga atggaattaa aatggcactt tgtgataaaa
     9181 aaataaccta aataattaaa aaattgttat ttttttgtaa caatctaaaa ttttattgcc
     9241 caaattataa cctgagatat gttggatgtg gagaatttgg aaacttgaaa cggatcagtc
     9301 agaaaactaa catatgttta aattaacata tgttttattt actaattctc tatacctata
     9361 ccatgcccat ctatccgaag atgcgaggtt aagcattcgc ccagtattgc ttacagtctt
     9421 gcctgtgagg tatttaccca tcactacata ccacataatg tagttttgca ggtatttagt
     9481 agctacgccc ctaaatttga aatatatcca attcgtgaat tcactagcct ttttcttgac
     9541 agtacttagg ctatatatgc ctcgcttttg cctatgactg gttactattt ctttatgctt
     9601 gattacattt cttctaacct ttttgaatgc ttttttatta ctgctacaca tgactccatc
     9661 ctttggcatc ttagccttca gaaattgctc tatttgttct gaacttagct ttttgctttc
     9721 aaattttttg aataataagt tgccagctct gtctgtagca gccaatacgg ctactttctt
     9781 tggcttttgc ttcctagctc gcttcagctc ttctgcattt ttgtatattc tacctttttc
     9841 ggaataatcc atcaatagtt catccatctc tactaccccg aaaaaattga tattatcttc
     9901 gaaactttgt aaagctgcca atatcttgtg tcgccaagcg aagcttgttg gtagactgat
     9961 gcccacctct ttagcacaag ctctgagact tttgcctgct accatacatc tgatgtagtc
    10021 gagcattttc tctttctttt gtaacctgta aacaaaagta cctgtggtaa ctctaaactg
    10081 tctgccacag tctttgcagc gatagttttg tatcccattt tgtaagccat ttttaataat
    10141 gtgattacta cgacagtctg ggcatagtgg cggcgcactg agtattttca gaccttgata
    10201 atcgactaca tcggcttcta attttttcat tagagaataa aggttgtcta agaattcttt
    10261 attctcttcc ttactcatag caagatattt actggtgttt tctacaattt ttttcatgaa
    10321 ttaaaatttt tataaaaata taaaaattat ttaacatata aacttgaaaa actttaacat
    10381 ttattaattt cattcaacaa aatatcattt atgaatatat tttagaatat
//
LOCUS       DAOPRN010000064        10316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_5459_length_10316_cov_110.763278, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000064 DAOPRN010000000
VERSION     DAOPRN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5459_length_10316_cov_110.763278"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..478)
                     /gene="rpmB"
                     /locus_tag="PKI65_06970"
     CDS             complement(239..478)
                     /gene="rpmB"
                     /locus_tag="PKI65_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOB78218.1"
                     /translation="MSKVCQISGKKSIVGNKVSHSNRKTKRRFYPNLQTKKFYWPEAN
                     KWITLKISTSVMRDINKKGIDAVMKESIKSGKFRD"
     gene            835..2136
                     /gene="metK"
                     /locus_tag="PKI65_06975"
     CDS             835..2136
                     /gene="metK"
                     /locus_tag="PKI65_06975"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOB78219.1"
                     /translation="MSYLFTSESVSEGHPDKIADQISDALLDEFLKQDPESKVACEIL
                     VTTGLIIISGEVTTDAWVDHHNIARKVVKNIGYTKSSYCFEYSSCGVISTLHEQSPDI
                     RMGVIKEKPEEQGAGDQGMMFGYACNEMKNYMPISIEVANILLQELANIRKEGKVMQY
                     LGPDSKSQVTLQYNDHQVATKIHTIVISTQHDDIWNTSEEMQEAIEKDIRTYLFPRVK
                     ASNVVPEEVRKLFDQNDFKLLINPTGKFVIGGPHGDTGLTGRKIIVDTYGGHGAHGGG
                     SFSGKDPSKVDRSGTYAMRYIAKNAVAAGLADKILLQVAYAIGVPQPLSLFANTYGTA
                     KVNMSDIEIAEKLLHLFDLTPYGIITKFQLKNPIYLPTATYGHFGRDVYEKEIEVSSR
                     HIDAQKRIVNGQTKYFKNITFFPWEKLDSVELIRNEFKIKL"
     gene            complement(2215..3828)
                     /locus_tag="PKI65_06980"
     CDS             complement(2215..3828)
                     /locus_tag="PKI65_06980"
                     /inference="COORDINATES: protein motif:HMM:NF016201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HOB78220.1"
                     /translation="MYLIDQHTKAIMEGCKERAREAGLQFADETLEYIVTNRDLLDLS
                     PKFMIPTLYDYWVNDVEVLQQYAKYKLYPTNPFETVINSRPAISFYNDNNPDWLNIMI
                     FYHVLAHIDFFQNNIYFSHTWNDDFVGVALADKRLINLYRSKYGRWVDYVIEFARSID
                     NIVGFYSIIFNQYLEETHKDPPPKLDFFFNHFLPNIIKANELERYKYLDHYNKRLEQY
                     GDRAENLFFSEVSIKYPEFEAFYDNYLKNQKKDQKYPDLLAFVNEQSPFLNKTNNRWM
                     KDIIVIIRNTALYFAPQIRTKIMNEGWASYWHDKLFRSDERISGHEVDYAKVNAEVTS
                     ISRIGLNPYAIGMRLYQYVEELANRGKLYRNYQELENIYQREVYDAKTGEGLTTIFDI
                     RKYFTDYNFIKIFIDQDFVDRHQLFVVGKQYNPNEQVIDYYVKSRNAEDYKQMLIENL
                     YHPPVIMPDLVRTNENQLYLIHKFEGKPLVQEFIPEVLIGLEYLWGGDAILETTQIII
                     KPTDDGSYLEESKKLRYICHNRNVKVEEI"
     gene            4081..5025
                     /gene="ftsY"
                     /locus_tag="PKI65_06985"
     CDS             4081..5025
                     /gene="ftsY"
                     /locus_tag="PKI65_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOB78221.1"
                     /translation="MSLFNIFKKNNKEAIDKGLEATKTSLFSKIHRLIVGKTSIDDEL
                     LDELEEILIEADVGVKTTIKIIDLIEEKAKQTRYTSNEELNKIIHSSIREILEQSLSN
                     MPKIEDIDAKPYVILVVGVNGVGKTTTIGKLAYQFKNANKKVILGAADTFRAAAVDQL
                     KLWAQRVGVDIVERGMNVDPGSVAYEATNIAKSKDYDVVIIDTAGRLHNKTNLMNELA
                     KINRVIKKIIPDAPHEVLLVLDASTGQNAIEQAKQFLSITNVNAIALTKLDGTAKGGI
                     VVAIADELKIPIKYIGVGENPEDLQLFDSKIFVDSLLS"
     gene            5022..6311
                     /gene="rimO"
                     /locus_tag="PKI65_06990"
     CDS             5022..6311
                     /gene="rimO"
                     /locus_tag="PKI65_06990"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOB78222.1"
                     /translation="MIDATLSIITLGCPKNQVDSERLAAALSPYFNVKHESPIADYVI
                     INTCSFILPAREESINMILDYIDFKKQGLIKKLIVIGCMPQIHKEELQKNLPEVDIFA
                     KINEYDTLVKTLSENQDAKIGQERILSTPNHYAYLKIADGCDHSCSFCAIPLIRGRYR
                     SIPIEILIDEAKTLAAKGVKELIIIAQDTTYYGLDIYHEQKLPELLDAVASLNLFPWI
                     RLLYTYPINFPLELIDVFNTHKSIVPYIHLPLQHCDHEILINMRRPFDEESIKQLIYQ
                     LKSKIPNLAIRTEFITGYPIETTQIFNKLKKFIKEMQFERIGVFDYSDEEGTFAALLP
                     QLKVSTRIKRAQELINIHEELSFLRNKSLIGKTIKAMVDKKNEETGIYEARSQWDAPE
                     IDQIVFIFSKKELNPGDIVDVYIDDAGIFDLSGIYKM"
     gene            complement(6343..8256)
                     /locus_tag="PKI65_06995"
     CDS             complement(6343..8256)
                     /locus_tag="PKI65_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOB78223.1"
                     /translation="MRSDIHDILQKYWGYTRFRPLQEEIIRSVLSGKDTLALMPTGGG
                     KSITFQVPALALEGMAIIISPLIALMVDQVENLQQRNIEAAAIHSGLTQREIELILHR
                     CIYKKDIKLLYVSPERLQTQSFREKLAKMSISFVAVDEAHCISQWGYDFRPPYLQIAS
                     IRQYFPDIPILAVTATATPRVIDDIIDKLELKSPQIFKQSFYRENLIYNVIETEDKIG
                     KILKLLKEFKGTSIIYVRNRKKTVTIAKHLQSLGFSAIYYHAGLSADEREKRQKAWIN
                     NRYRIMVATNAFGMGIDKPDVRLVIHLDIPDSIESYFQEAGRAGRDLKLSYAFLLYHP
                     TDIEDFRNNIKKNFPTLETIKQAYKLLCQYYQLNIGEGENEIFQFYINELLKDQDISA
                     VDFYNSILILEKAGYLALSEGIKTKSRLHFLIDKNTLYKFQLEHPEWNEFIKNLTRTY
                     SNLFSEYTNISEDNIALKTKMSINEVQNSLLALKEQGILDYLPSAGENTITFLKACID
                     EADIILPNMIYEDRKKIATSNAEALISYILNQTKCRSQILLEYFGEESLIRCGRCDVC
                     VARNEVNLSKLEMDKIINLIKPLLRDKPMNESEILEQFSIMDEIKIMKVISWLIETDK
                     IIRLPDMRLRWKR"
     gene            8571..10196
                     /locus_tag="PKI65_07000"
     CDS             8571..10196
                     /locus_tag="PKI65_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOB78224.1"
                     /translation="MKRFTKNRKIIPVSLIIMLLTGFNAINLNAQEISFTFTANHTCE
                     YAALDSVLVENLTQGGDTTLYYPDTVLTLVLTGIDQFLTGSNNFYVSQNYPNPFETKT
                     KIDIFLPECDDITIDVYDLLGRKVANYEGSHESGMQHFTFIGGNSKNYILTVSSKKYL
                     QYIQMIQFGEVSSGLPYLEYNGSTTMNSIKIKQKSLQSLFPYNLGDQLKFTGYVSGDY
                     ASITDNPTTSQEYIFDINHIVPTTPTEGTHIAGEDQIEWHWNTVDDADGYKYNTVNDY
                     TSATDNGTSTSYTQTSLACETNYTLYVWAYNSCGASSSLELNEQTSDCHFICGTSTVT
                     YDGVTYNTVEIGSQCWLKENLRTTKYNDNSSIPEVTDPSTWTSTTSGAYCCYDNNPSN
                     CDTYGALYNWYAVNTGKLCPRGWHVPSDDEWYTLTNYLSANSQYWCGGNSNYIAKSLA
                     STSTLWNTYTVTCTVGNDLSVNNSTGFSALPGGYCNYAGSFDYLSERGYWWSSTEKSA
                     SEAYYRLMYYNSSNVGRTSFDKRSGLSVRCLCD"
BASE COUNT         3603 a         1571 c         1567 g         3575 t
ORIGIN      
        1 aatttaataa attttctgat attaatcctg tataaatttt aacagactaa atattagaat
       61 ataaaaaagg tcaactgttt ttttaacagt tgaccttttt taagattatt tcatcacctt
      121 aatgtttatc acaaaattta tgttctggac cataactatg atggccatat ttatgagctt
      181 ggcaattatg catcatttca cttttactat gtcggcaaga ttgcctcatc atcaaaaact
      241 aatctctaaa ttttccagat ttaatagatt ctttcattac agcatctata ccttttttat
      301 taatatcacg cataacactt gttgatattt tcaaagtaat ccacttgtta gcttcaggcc
      361 aataaaattt ctttgtctgt aaatttggat aaaaacgacg tttagttttt ctattggaat
      421 gagaaacttt atttcccaca attgactttt ttccagatat ttggcaaact ttagacataa
      481 ttttaaattt tagattgcaa aattacaaaa aaaaatttag ttaaaaaaat aattaatcca
      541 taaaatttaa agatgaaata tattatacta gatcaaaccc tattgtcaaa gcttttcttg
      601 aattttttct cattttttta aattttaata aattaaggta atatataaag attaactact
      661 ttttaatcct ttcattataa ttaactttct atatataaca ttcaaattat attctcgact
      721 tcttcgtata agaatgttta ttacctattt atctttatta ctcaaaaaaa agtaattttt
      781 aattttataa ttttaaaata aataattaat ttcgcaagct aataatataa agatatgtca
      841 tatttattta cttcagagag cgtatcagaa ggacatcctg ataagatagc tgatcagatc
      901 agtgatgctc ttctagatga atttctcaag caagatcctg aatctaaagt agcatgtgaa
      961 attttagtta ctacaggcct aataataata tctggagagg taactactga tgcatgggta
     1021 gatcatcaca atatagctag aaaagttgtt aaaaacattg gatatactaa aagttcgtat
     1081 tgtttcgaat acagctcttg tggagttatt tctactcttc atgaacaatc tcctgatatt
     1141 agaatgggag ttataaaaga aaaacccgaa gaacaaggtg ctggtgatca aggcatgatg
     1201 tttggttatg catgtaatga aatgaaaaat tacatgccta tttctattga agttgctaat
     1261 atattgcttc aagagctagc aaatattaga aaagaaggca aagtaatgca gtatctagga
     1321 cctgatagca agtctcaagt caccttgcaa tataatgatc atcaggtggc tactaagatt
     1381 catactatag ttatttctac acaacacgat gatatatgga atacttccga agagatgcaa
     1441 gaagcaattg aaaaagatat aaggacttac ctttttccaa gagtaaaagc gtcaaatgtt
     1501 gttcccgaag aggtaagaaa attgttcgat caaaatgatt ttaaattatt aataaatcca
     1561 actggcaaat ttgttatagg tggacctcat ggagatacag gattgactgg tagaaaaata
     1621 attgtagata cttatggggg acatggcgct catggtggtg gctccttcag tggaaaagac
     1681 ccatcaaaag tagatcgctc aggcacttac gcaatgagat atatagccaa aaatgctgtg
     1741 gctgccggct tagctgataa aattttatta caagttgcct atgctatagg tgtacctcaa
     1801 ccactttcat tatttgcaaa tacttatggt acagctaaag taaatatgag tgatatagaa
     1861 atagcagaaa agctattaca tctttttgat cttacaccat atggtataat tactaaattt
     1921 caattaaaaa atcctatata tctccctact gctacttatg gacattttgg tagagatgtt
     1981 tatgaaaaag aaattgaggt tagtagtaga catatagatg cccaaaaacg tatcgtaaat
     2041 ggtcaaacta aatatttcaa aaatataact tttttcccat gggaaaaact cgacagtgta
     2101 gaactgataa gaaatgaatt taaaataaaa ctctaacttt tttagtatta tttaataatt
     2161 tataaataaa aaaaatcgtg ttgtaattga tttaattatt aactaactgc taatttatat
     2221 ttcctctacc tttacattac gattatggca aatatatctc agttttttac tttcttctag
     2281 atagctacca tcatctgtag gctttattat tatttgggta gtttctaaaa ttgcatcacc
     2341 tccccataaa tattctaaac cgatgagaac ttctggtata aactcctgta ctagtggctt
     2401 accttcaaat ttatgaatta gatataattg attttcattg gttctaacca aatctggcat
     2461 tattactggt ggatgataaa gattttctat taacatttgc ttgtaatcct cagcgtttcg
     2521 gcttttcaca tagtagtcta tcacttgctc attaggattg tattgcttgc ctactacaaa
     2581 taattggtgt ctatctacaa aatcctgatc tataaaaatt tttataaaat tataatctgt
     2641 gaaatatttt ctaatatcaa aaatggtagt taagccttca cctgttttag catcataaac
     2701 ttctctttga taaatatttt ctaattcttg ataatttctg tatagtttgc ctctattagc
     2761 taactcttct acatattgat atagtctcat gccaatagca taaggattta atcctattcg
     2821 agaaatagaa gttacctcag catttacttt agcataatct acttcatgac cacttattcg
     2881 ctcatcgctt ctaaaaagtt tatcatgcca ataacttgcc caaccttcat tcattatttt
     2941 agtcctaatc tgtggagcaa aataaagtgc agtattacgt attatcacta ttatatcttt
     3001 catccaacga ttattagttt tatttagaaa agggctttgt tcatttacaa aagctaaaag
     3061 atcgggatat ttttggtctt ttttttgatt ttttaaataa ttatcataaa atgcttcaaa
     3121 ttctggatat ttaatcgaga cttctgagaa aaataaattt tcggctctat ctccatattg
     3181 ctctaaacgt ttattataat gatctaagta tttatatctt tctaattcat ttgctttgat
     3241 gatgttaggt aaaaaatgat tgaagaaaaa atctaatttt ggtggtggat ctttatgtgt
     3301 ttcttcgaga tattgattaa atattatcga ataaaaacct actatattat ctatgcttct
     3361 agcaaattct atcacatagt ctacccatct accatattta cttcgatata aatttatcaa
     3421 tcttttatct gctagtgcca ctcccacgaa atcatcattc caagtatgtg aaaagtaaat
     3481 attattttga aaaaaatcta tatgagctag tacatgataa aaaatcatta tgtttaacca
     3541 atcaggatta ttgtcattat aaaaagatat tgcaggtcta ctattgatca cagtttcgaa
     3601 aggatttgtg ggatacagtt tgtatttagc atattgctgt aatacctcta catcatttac
     3661 ccagtaatca tagagtgttg ggatcataaa tttgggagat aagtctagta aatctcgatt
     3721 agttactata tattcgagcg tctcatcagc aaattgtagt cctgcttctc ttgctcgctc
     3781 cttacatcct tccattatag ctttagtatg ttgatctatt aaatacattt tattttttta
     3841 tacaaatata atttaaaata tttaatatag attgttttta taaatattca aaaatcaaac
     3901 taatcaatat aaagatagct aaaccattat ttataatttt taaaatcaat taattttaaa
     3961 acaaatttat tttttttaaa aaaaatatta tataattaca atctattcga gtataatcgt
     4021 aaacttaatt ttaatattta tagatttaaa ccttaaatta tttgtaattt tgcaaaaaaa
     4081 atgagtttat ttaatatttt taaaaaaaat aataaggaag ctatagataa aggattagaa
     4141 gctactaaaa ctagtttgtt ctcaaaaata catcgtttaa ttgtaggcaa aacctccata
     4201 gatgatgaat tgctagatga attagaagaa attttgatag aagctgatgt aggggtaaaa
     4261 actactatta aaattattga tttaatagaa gaaaaggcaa aacaaacaag gtatacgtct
     4321 aatgaggagt taaataaaat aattcactct agtattagag aaattctaga gcagagttta
     4381 tctaatatgc ccaaaataga agacatagat gccaaacctt atgtgatact tgtagtaggt
     4441 gtaaatggtg taggcaaaac tactactatt ggcaagttag cttatcaatt taaaaatgca
     4501 aacaaaaaag taattttagg agcagcagat acatttagag cagctgccgt agatcaatta
     4561 aaactttggg ctcaacgcgt aggagtagat attgtagaaa gaggtatgaa tgtagatcca
     4621 ggctctgtag cttatgaagc tactaatatt gctaaatcta aagattatga tgtggtgata
     4681 atagatactg ctggtagatt gcataataaa actaatttga tgaatgagct agcaaagatt
     4741 aatcgagtaa ttaaaaaaat aatccctgat gcacctcacg aagtattgtt ggtattagat
     4801 gcatctactg gtcagaatgc tatcgagcaa gctaagcaat tcctgtcaat tacaaatgta
     4861 aatgctatag ccctaactaa actagatggt actgctaaag gaggcatcgt agtggctata
     4921 gctgatgaat tgaaaatacc tattaagtat attggtgtcg gtgaaaatcc agaagaccta
     4981 caactctttg acagtaaaat atttgtagat agcctattat catgatagat gcaactttat
     5041 caattataac attaggatgt ccaaaaaatc aggtcgatag tgaaaggctg gcagcagctt
     5101 tatctccata ttttaatgta aaacatgaat ctccaattgc tgattatgtg attattaaca
     5161 catgttcttt tatcttacct gctcgagaag agtctattaa tatgatcctc gattatatag
     5221 attttaaaaa acaaggactg ataaaaaaat taattgtgat aggctgcatg ccccaaatac
     5281 ataaggaaga attacaaaaa aatttacccg aagttgatat ctttgctaaa ataaatgaat
     5341 atgatacttt agtaaaaaca ctctcagaaa atcaagatgc taaaatagga caagagcgaa
     5401 tactctctac gcccaatcat tatgcatatc taaaaatagc tgatggctgt gatcactctt
     5461 gctctttttg tgctatacca ttaataagag gtaggtacag atctattccg attgaaatat
     5521 taatcgatga ggctaaaaca ttagctgcta aaggagtaaa agaactgatc ataattgctc
     5581 aggatactac ttattacgga ttagatattt atcatgagca gaaattacca gaactcttag
     5641 atgctgtggc ttcattaaat ctttttcctt ggattagact tttgtatact tatcctatta
     5701 attttccttt agaacttatc gatgttttta atactcataa atctattgtt ccttatattc
     5761 atttaccact tcagcattgc gaccatgaaa tattaataaa tatgcgtcgc ccttttgatg
     5821 aagagtctat taaacaatta atttatcaac taaaatctaa aatacccaat ttagccattc
     5881 gtacagaatt cataacaggt tatccaatag aaacaactca aatatttaat aaattgaaaa
     5941 aatttattaa agaaatgcaa tttgaacgta taggggtatt tgattactct gatgaagaag
     6001 gtacgttcgc agctctattg ccacaattaa aagtatcgac tagaataaaa agagcccaag
     6061 aattgataaa tatacacgaa gaattatctt ttttgagaaa taaaagttta ataggcaaaa
     6121 ccataaaagc tatggtagac aaaaaaaatg aggaaacagg tatttatgaa gcacgcagtc
     6181 aatgggatgc tccagagata gatcaaattg tttttatttt ttctaaaaaa gaattaaacc
     6241 ccggggatat tgtagatgtt tatatagatg atgctggtat cttcgacctg agtggtatat
     6301 acaaaatgta ataaagcaat cgattaatgt gtaaaaatat tttcacctct tccatcttag
     6361 tcgcatatct ggtaatctta tgattttatc tgtctcaata agccaagaaa tcactttcat
     6421 tatctttatt tcgtccatta ttgaaaattg ctctaatatt tcactttcat tcattggctt
     6481 atcacgtaga agcggtttta tcagattaat tattttatcc atttctaatt ttgataaatt
     6541 tacttcattt cttgccacac atacatcaca tctgccacat cttattaatg actcttcacc
     6601 gaaatattct aataagattt gactacgaca tttggtttgg ttcaatatat atgatattag
     6661 tgcttctgca ttagatgtgg ctattttttt tctatcttca tagatcatat ttggtaatat
     6721 tatgtcagct tcatcaatac atgcttttag aaaggtaatc gtattttcgc ctgcagatgg
     6781 caagtaatct aaaatacctt gttctttgag agctaaaaga gaattttgta cttcgtttat
     6841 tgacattttt gtttttagtg caatattatc ttctgatata ttagtatatt ctgagaaaag
     6901 gtttgaatat gtgcgtgtta gattttttat aaattcattc cactctggat gttctaactg
     6961 aaatttgtag agagtgtttt tatcaatcaa aaaatgtaat ctggattttg tttttatccc
     7021 ttcactcaga gccaaatatc cagctttttc tagtatcaaa atactattat aaaaatctac
     7081 tgctgagata tcttgatcct ttaataactc attaatataa aattgaaaaa tttcattttc
     7141 accttctcct atatttaact gataatattg acagagtagc ttatatgctt gttttattgt
     7201 ctctaatgtc ggaaagtttt ttttgatatt atttctgaaa tcttcaatat ctgtaggatg
     7261 ataaagaaga aaagcataag aaagcttaag gtctctaccc gctctaccag cttcttgaaa
     7321 ataagattct atactatcgg gtatatctaa atgtataacc agtcgcacat caggtttgtc
     7381 tatacccata ccaaaggcat tggtagccac cattatgcga tatctattat ttatccacgc
     7441 cttttgtctc ttttcgcgtt catctgcaga tagtcctgca tggtaatata tagccgaaaa
     7501 acctaatgat tgtaaatgtt tagcaatggt tacagttttt tttctatttc tcacatatat
     7561 gatcgaggtg cctttgaatt cttttaataa ttttaatatt ttccctattt tatcttcagt
     7621 ttctatcaca ttataaatga gattttcacg ataaaatgat tgtttaaata tttgtggact
     7681 tttaagttct aatttatcaa taatatcatc tattactctg ggggtagctg tagcggtaac
     7741 agcaagaata ggaatatcgg ggaaatattg tctgatagaa gctatttgta aatatggagg
     7801 tctaaagtca taaccccatt gagatataca atgagcttca tctacagcta caaaacttat
     7861 tgacatttta gctaattttt ctctaaatga ttgtgtctgt agtctctcgg gggatacata
     7921 tagcagctta atatcttttt tataaataca acgatgtaat ataagctcaa tctctctctg
     7981 cgtgagcccg ctatgtatag cagcagcttc gatattacgt tgttgtaaat tttctacttg
     8041 atctaccatg agagctatca gaggtgaaat aataatagcc atgccttcta aagctagtgc
     8101 tggcacttga aaagttatac ttttgccccc acctgttggc atcaatgcta atgtatcttt
     8161 accagacaaa acagatctta tgatctcttc ctgcaaagga cgaaatctcg tgtaacccca
     8221 atacttttgt aaaatatcat gtatgtctga tctcacaatt ttagaaaata tttttgttaa
     8281 attacaaatt ttttattaca aatgttataa ttatattgca tcaaaataaa tataaaaatc
     8341 attgataggg acaattcacg atttgcccaa tgggaaataa aataaacgca tcgtgtgagt
     8401 gtataatcat atgccccaaa tcatacgcct ccaatcaaaa ttaattaaat cataaaaata
     8461 tttatttata ttaaaaaaat tatataaatt tgtatattaa acccataaaa tcttttactg
     8521 attaaataaa atataaaata aatttgaaaa aattataaaa ataaaaaatt atgaaaaggt
     8581 ttacaaaaaa cagaaaaatt attcctgtga gtctgattat tatgttgctt acaggattta
     8641 atgcgatcaa ccttaatgcc caggaaattt cctttacctt cacagcaaac cacacttgtg
     8701 agtatgctgc attggatagt gttttagttg aaaacttgac gcagggagga gataccacct
     8761 tgtattatcc tgatacagtg ctaaccttag ttttgacagg tatagatcaa tttttaactg
     8821 gatcaaataa tttttatgtg tctcagaatt atcccaaccc ctttgaaaca aaaacaaaaa
     8881 tagatatttt tttgcctgaa tgcgatgata ttactataga tgtttatgac cttttgggaa
     8941 gaaaagtagc caattacgaa ggtagccatg aaagtggcat gcaacatttt acctttattg
     9001 gaggtaactc aaaaaactac attttaactg ttagttcaaa gaaatatttg cagtatattc
     9061 agatgataca atttggagaa gtaagtagtg ggttacctta tttagaatat aatggatcaa
     9121 ctacaatgaa ttcaataaaa ataaaacaaa aatctttgca atcacttttt ccatataatt
     9181 tgggagatca attaaaattt acaggttacg tttctggaga ttatgcttcg atcacagata
     9241 atccgacaac aagtcaagaa tatatttttg acatcaatca catagtaccc actactccca
     9301 cggaagggac acatatagct ggtgaagatc aaatagaatg gcactggaac acggtagatg
     9361 atgcagatgg ttataagtac aataccgtta atgattatac tagtgccaca gataatggga
     9421 ctagtacatc atacacacaa actagtctcg catgtgaaac caactacacc ctttatgtat
     9481 gggcatataa cagttgtggc gcatcctcat ctcttgaact aaatgaacaa acctctgatt
     9541 gtcatttcat atgtggtaca tctactgtca cttatgatgg tgtcacatac aacactgtag
     9601 aaataggcag tcaatgttgg cttaaggaga atctacgaac aacaaaatac aatgataata
     9661 gttcaatacc agaggtaaca gatccttcta catggacatc taccacttca ggtgcatatt
     9721 gttgttatga taataatcca tctaattgtg atacttatgg tgctttatac aattggtatg
     9781 cagtaaatac aggtaaatta tgcccaagag ggtggcatgt accatcggat gatgagtggt
     9841 atacattgac taattactta agtgcaaata gtcaatattg gtgtggaggt aatagcaatt
     9901 acatagcgaa atcacttgct agcacttcaa cattatggaa tacatacacg gtaacttgta
     9961 cagtaggtaa cgatttatct gttaataata gcactggttt ctctgctttg cctggtggct
    10021 actgtaacta cgctggttcg ttcgattatc taagtgaacg cggctactgg tggagtagta
    10081 ctgagaaaag tgctagcgaa gcatattacc gtttaatgta ctacaatagc agtaatgtcg
    10141 gccgcaccag cttcgataag agatccggat tgtctgtacg ctgcctctgt gattaagaca
    10201 gttaaaagtt aaaagttcat aaagtttata aagttaaaag gtgaaaattg aaagatttga
    10261 agatattatc gcatggcaaa aagcaaagga tatgactgtt tttatttata aggatt
//
LOCUS       DAOPRN010000065        10021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_5656_length_10021_cov_87.696568, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000065 DAOPRN010000000
VERSION     DAOPRN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5656_length_10021_cov_87.696568"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2372)
                     /locus_tag="PKI65_07005"
     CDS             complement(<1..2372)
                     /locus_tag="PKI65_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOB78225.1"
                     /translation="MKKVVLISILLILFSNFNFSQQWTFLTDDNNAKAEYTLDEAKSN
                     NKDLTINFTLNAYRLKEITINNNVSYIVESPNGARILKAGAPDLPLYAQSVIIPDTGS
                     MEVIINKVDFVEIQKIDIAPSKGNLLRTVNPDSVPYSFGSEYQQNSFFPTDIVYLRDP
                     YILRDFRGQAIVVQPFQYNPVTKTLRIYTNIDFTVRATQGSGINEFHRNKALQSIDRE
                     FNEIYKRQFLNYGSSLKYTPLNDNPGRMLIISYSSFISAMQPFIEWKIMKGIPCEIVD
                     VSTIGNNATSIKDYVTNYYNTNGLTYLLLVGDFAQVTSPTGNYSGVTGAKDNSYAYIT
                     GNDHYQEFFVGRFSAESVTDLQTQVTRSINYEKNPTTGTWLEGTLGIASSEGPGHNSE
                     YDYQHIRNIMNDLMNYNYTTYYELYDGSQGGQDAPGNPTATNVSTIVNNGVGNIFYCG
                     HGDDTYWVTTNFSNSNVNTLTNYNKLPFIYSVACVVGHFNVGTCFCEAWMRANKTNGP
                     TGAIGIFGSTINQSWAPPMTAQDEMADILVESYTSNIKRTFAGIGINGCFKMNDVHAD
                     YNMTDTWTVFGDPSIVLRTKNPMNMTVSHPSSINTGTSNINVTCNVNGAYVSITLNNQ
                     ILGTGYVSNGTANITLNPAPSNGGETLKVCVTAYNYIPYIGDILVAGTSTNPLNFTAT
                     AISQSQIDLSWSLNSSNNPVLLVYNTTNTFGTPTSGTTYNVGQTINGGGTIIYNGSNT
                     TFSHTGLNSNTTYYYKIFSIMTGNTYSTGVTAQATTLPDPISGFSLDFEA"
     gene            complement(2788..3927)
                     /locus_tag="PKI65_07010"
     CDS             complement(2788..3927)
                     /locus_tag="PKI65_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOB78226.1"
                     /translation="MFEQLSYSTLQGYWWLIISILGATLVFMMFVLGGQTLIYTLGKK
                     EPQLSLIIKEIGIRWDLTFTNLVVFCGTIFAAFPLFYSTSFGGAYWLWMLILFTFVLQ
                     AVSYGFRNKAKNFLGAKTYEWFLFLNGLFGTISLGVIIGTLFTGGNFVHDEMNFMRWT
                     TNWHGIEAFFNIKNLLLGLSIFFLARTLALLYLINRVSDIHIQDASKKHLIINAPIFF
                     VLFFTFILCICLGNGYTLDPETQIVFIQKYKYFKNFIELPWLGTIFLIGILLLIFGVL
                     IPFLKNKKLENKGIWLAGFGCFFTILSLFLCLGYNNTVFYPSLVDMQSSLTIYNASSS
                     KYTLVVMSYVSLLIPFIAAYIIYTSRLLLKKSFTANETSSDNNIF"
     gene            complement(3962..5515)
                     /locus_tag="PKI65_07015"
     CDS             complement(3962..5515)
                     /locus_tag="PKI65_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOB78227.1"
                     /translation="MDELISLVNWSRGQFALTAMYHWIFVPITLGLTIMIAIMHTHYY
                     KTDNDEWKKMTKFWMKLLIINFILGVATGIILVFEFGTNWSNYSWFVGDIFGAPLAIE
                     GILAFFMEATFIAVMIFGWDRVSKKLHLISTWLVAIGSNLSALWILIANAWMQHPIGT
                     VFNPATARNEMINFWDIALSPVAINKFLHTTTSSYVVASLFVISVSSWYLLKKRHIFI
                     AKKSMLIAAIFGFISSIMTIFTGDSSVYQVAQKQPMKLAAMEGLYEGKTSAGLVTIGI
                     LNPKKDINNDEKPYYFKIEIPYLLSLLSYRDADAYVPGINDLIYGNEEQQILSAEEKI
                     ARGNIAIETLKQYQNAKKQNDTNALANLGKKFDPNTKVGDYFLNNYFRYFGYGYLSSP
                     HELIPNIALTFYSFHIMVSLGFLFILLFLLVFIYVWKDTIENKNILLYISLWSVLFGF
                     IASQSGWIVSEVGRQPWIIQDLMPTVAGVTQLSVSNVQITFILFAIIFTTLLIAELSI
                     MFNQIRKGP"
     gene            complement(5517..5729)
                     /locus_tag="PKI65_07020"
     CDS             complement(5517..5729)
                     /locus_tag="PKI65_07020"
                     /inference="COORDINATES: protein motif:HMM:NF026249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOB78228.1"
                     /translation="MLKKITYLYIECFRNISKTGRQLLVILFPNFFVFFIILKLIFLP
                     NILKTNYNTDKDIADHVIEQLATKIK"
     gene            complement(5996..7837)
                     /gene="glmS"
                     /locus_tag="PKI65_07025"
     CDS             complement(5996..7837)
                     /gene="glmS"
                     /locus_tag="PKI65_07025"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOB78229.1"
                     /translation="MCGIIGYIGYRQAFPILINGLHRLEYRGYDSAGISLLNNGLKIY
                     KTHGKVSDLEACVIDKDLSGTVGMGHTRWATHGEPTDINAHPHFSPSHKLAIIHNGII
                     ENYKTLKEELISRDYQFKSETDTEVIIHLVEDIIKNEHVDIVEATRLALSQVIGAFAI
                     VILSENNPDMLIAAKRGSPLVIGVGDDEYFVASDPSPIIEYTNKVVYLEDDEIAVIQK
                     NKDLKIITIKNLEKTPYIQQLEMSLSQIEKGGYEHFMLKEIFEQPQSVKDSMRGRLSL
                     AKQTIALGGIVEYENRLLTAPRIILVGCGTSWHAGLVGEYLLEDLARIPVEVEYASEF
                     RYRNPVLHENDVVIAISQSGETADTLAAVKLAKSKGAIPLGICNVVGSSIARETVAGS
                     YTHAGPEIGVASTKAFTAQITILTMMALMLGYKKGVIPKSRFIQHLHDLNEIAEKMQI
                     TLEKTNEPIKHIASQFKDARNFLYLGRGYSYPVALEGALKLKEISYIHAEGYPAAEMK
                     HGPIALIDENMPVVFIAPQSNIYDKTVSNIQEVKARKGIIISIVSEGDDTVKKLSDYY
                     IEIPKTQEFLVPLVATIPLQLLAYHIAVLRGCNVDQPRNLAKSVTVE"
     gene            8218..9117
                     /locus_tag="PKI65_07030"
     CDS             8218..9117
                     /locus_tag="PKI65_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOB78230.1"
                     /translation="MLKNNKFNIFLISLVLLCLCSCQKKEKEYFESLGNHKSFLYIDH
                     REDTIYTIKRSNYDVKIYLFANKNFFTRGTVLLLHGWNLSPLSWCDQTNFCDELLASG
                     YHVLIPDLGKSVYATQYFKETVPSMTKYPQMPFILDTLINFLVDYIKIIDTSHYNFLV
                     GYSMGARGALAIAENTNNLFSALVLLAGEYDQTLMPDDNIMTMYYGSYQKFPDRWEGN
                     DNLLRNIDKVNIPVLILHGLDDDVIPVAQSDTLAKVLEKHNKFFIKIYYYNYGHGFDF
                     VQKTINNIKSFFEKVIYPWNLDV"
     gene            9689..>10021
                     /locus_tag="PKI65_07035"
     CDS             9689..>10021
                     /locus_tag="PKI65_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78231.1"
                     /translation="MNKSVKTRSKSQKEKIKLNNKSWITKITEYPDKKLCWFLFLLAT
                     IIYLPTITFDYVLDDGIVIHENKFTQKGIKGIKEIFTHDSFVGYFGEEVKYVAGGRYR
                     PFTQFIFAI"
BASE COUNT         3480 a         1514 c         1458 g         3569 t
ORIGIN      
        1 catgcttcga aatctagact aaatccagaa ataggatctg gtaaagtagt agcctgagcc
       61 gtaacgccag tagaatatgt attgccagtc attattgaga aaattttata atagtaagtt
      121 gtattagagt ttaacccagt atgtgaaaaa gttgtattag aaccattata aataatggta
      181 ccaccaccat ttatagtttg accaacattg taagttgtgc cagatgtagg agtgccaaag
      241 gtatttgtgg tattataaac taatagtact ggattattgc tagagttcaa actccaggat
      301 aaatctattt gagattgaga aatagctgta gcagtgaagt ttaatggatt agtagatgta
      361 ccagcaacta aaatatcacc aatataaggg atatagttat atgcagtaac acaaactttc
      421 aatgtttcgc caccattact tggagcggga tttaaggtta tattcgctgt accattagat
      481 acgtaaccag tacctaatat ttgattattc aaagtaatag aaacataagc tccattgaca
      541 ttgcatgtta cattaatatt tgatgtgccg gtattaatag atgaagggtg tgatacagtc
      601 atattcatag gattttttgt acgtaatact atactaggat ctccaaatac tgtccatgta
      661 tcagtcatat tataatcagc atgaacatca ttcatcttga agcaaccatt tatgccaata
      721 ccagcaaagg ttcttttaat attgcttgta taactttcta ctaagatgtc tgccatctca
      781 tcttgagctg tcataggtgg agcccatgat tgattaatag tagatccaaa aatgccaata
      841 gcgccagtgg gtccattagt tttgttagct ctcatccaag cctcacaaaa acaagtaccc
      901 acattgaaat gacctactac acaagcaact gaataaataa aaggtaattt attataattt
      961 gttaaagtat taacattaga gttagaaaaa ttagttgtaa cccagtatgt atcatcacca
     1021 tgtccacaat aaaaaatgtt accaacgcca ttgttaacaa ttgtcgatac attagttgca
     1081 gtaggattgc ctggtgcatc ttgtccacct tggctaccat catatagttc gtagtatgta
     1141 gtgtaattat agttcattaa atcattcatt atatttctta tgtgctggta atcatactca
     1201 gaattatgac ctggaccttc gctagaagct atacctaatg tgccttctaa ccaagtgcca
     1261 gtagtgggat ttttttcata atttatacta cgagttacct gtgtttgtaa atctgtaaca
     1321 gattctgctg agaaacgccc tacaaaaaat tcctgataat gatcattacc cgttatataa
     1381 gcataactat tatctttggc accagttacg ccactataat ttccagtagg tgatgttact
     1441 tgtgcgaaat cacccactaa aagtaaatat gttagcccat ttgtattgta ataatttgta
     1501 acataatctt taatagaagt agcattatta ccaatagtag acacatctac tatttcgcaa
     1561 ggaatacctt tcattatttt ccattctatg aagggctgca tagctgagat aaaactagaa
     1621 tatgaaatta taagcattct acctggatta tcattaagtg gtgtgtattt cagtgaagaa
     1681 ccataattta gaaattgacg tttatatatt tcattaaatt ctcgatcaat agattgtaga
     1741 gctttatttc tatgaaattc attaatgccg ctaccttgag tagctctgac tgtgaagtca
     1801 atatttgtgt atatgcgcaa tgttttagtt actggattat actgaaatgg ttgaactact
     1861 atagcctgac ctctgaaatc tcttaagata tatggatctc gcaaataaac aatgtctgtt
     1921 gggaagaaac tattttgttg atattctgaa ccaaaagagt atggaacact atctgggttt
     1981 actgtacgta gtagattacc tttagatggg gctatatcta tcttttgtat ttctacaaag
     2041 tctaccttgt ttattattac ttccatagat cctgtatctg gtattataac ggattgagca
     2101 tataagggca aatctggagc cccagctttt agtatcctag ctccatttgg agattctact
     2161 atataagata cgttattatt aattgttatt tcctttaatc gataagcatt caaagtaaaa
     2221 tttatagtta aatctttatt attactctta gcttcatcta atgtgtattc tgcttttgcg
     2281 ttattatcat cagtcagaaa tgtccattgc tgactaaaat taaaattaga aaataaaatt
     2341 aataaaattg aaattaatac aacttttttc atacttttta tattttttaa gaaattatta
     2401 gatcaaaatt aataaaaaaa ttataaatta aatacaaatt aataaaatat gtgtttaatt
     2461 taaaaataaa tttaattaac atttgtgttc tttagtataa attgataaat ggttctataa
     2521 tgtttattgt gggtaaaaat atttcagatt gttacaaata ggttattttt ttaccccaaa
     2581 gtgccattta tagccctttt aattccatcc ttctcagcgt tctttgtgca ttctttgagt
     2641 actctgtggt taatattcaa ctgtccaata ataaaaaaac caattattaa taaatttatt
     2701 tttgtaaaat tacttttttt ttaatcttta cctatacaaa acgttataga accttttaat
     2761 ttttatatta tcttagatat attctattta aaatatatta ttgtcgctgg acgtctcatt
     2821 tgcagtaaac gattttttaa gtagaagtct cgacgtataa ataatgtaag cggctatgaa
     2881 tggtattaat agtgacacat aagacatcac taccagtgtg tatttactcg atgatgcgtt
     2941 gtatattgtt aagctggact gcatgtctac taacgatggg taaaaaacgg tattattgta
     3001 acctaagcaa agaaaaagag ataatattgt aaaaaaacat ccaaatccag ctaaccagat
     3061 ccccttattt tctaattttt tattttttag aaatggtatc aatactccaa atatcaataa
     3121 aagtattcct atcaaaaata ttgtacctag ccatggaagt tctatgaaat ttttaaaata
     3181 tttatatttt tgtatgaata ctatttgtgt ttctggatct aaggtgtatc cattacctag
     3241 gcagatacaa agtataaaag taaaaaaaag gacgaaaaaa attggtgcat tgataattaa
     3301 atgcttttta gatgcatcct ggatatgaat atctgatact ctatttatca aatataataa
     3361 agccaaagta cgagctaaaa agaaaataga taatcctaat aacaagtttt taatattaaa
     3421 aaaagcctcg atgccatgcc agtttgtagt ccacctcata aaattcattt catcatgtac
     3481 aaaattaccc cctgtaaata atgttcctat tattactcct agtgatatgg taccaaacaa
     3541 tccattgaga aatagaaacc attcataagt tttagctcct aaaaaatttt tagctttatt
     3601 tctgaatcca taagagacgg cttgcaagac aaaagtaaat aaaatgagca tccataacca
     3661 gtaagcacca ccgaagctag tactataaaa cagtggaaaa gcagcaaata tagtaccaca
     3721 gaaaaccacc aaattagtga atgttaaatc ccatcttatt cctatttctt ttataatcag
     3781 gcttagctgt ggttcttttt tgcctagagt atatattaat gtttgaccgc ctaaaacaaa
     3841 catcataaat actaatgtag ctcctaatat agaaataata agccaccaat atccctgtaa
     3901 tgtactataa cttaattgtt caaacatgat tttttttata actatttata ttaattaaaa
     3961 tttatggacc tttacgtatt tgattaaaca taatgctaag ctctgcaatt aatagtgtag
     4021 taaaaataat tgcaaaaagg atgaaagtaa tctgcacatt tgatacactc aattgtgtga
     4081 caccagctac agttggcatt agatcttgta tgatccatgg ttgtcgccct acttctgata
     4141 ctatccagcc tgattgcgaa gctataaatc caaacaatac tgaccataga ctgatgtata
     4201 ataatatgtt tttgttttct attgtatcct tccacacata aataaaaact agcaaaaaca
     4261 ataaaataaa taaaaaacct aaacttacca ttatatgaaa actataaaat gtcaaagcta
     4321 tattaggaat taactcatga ggactactta gataaccata accaaaatat ctaaaataat
     4381 tatttagaaa gtaatctccc actttagtat taggatcaaa ttttttacca agatttgcaa
     4441 gtgcattagt atcattttgt tttttagcat tttgatattg ctttaatgtt tctatagcta
     4501 tattacctcg agctatcttt tcttcggcac ttaatatttg ttgttcttca ttaccataaa
     4561 ttaaatcatt tattcctggt acataagcat cggcgtcacg atagctaaga agcgataaaa
     4621 gatacggaat ttctatttta aagtaataag gtttttcatc attattaata tcttttttag
     4681 gatttaaaat tccaatagtt accaatccag cacttgtttt gccttcgtac aagccctcca
     4741 tagctgctag tttcataggt tgcttctgtg ctacttgata cactgagcta tcaccagtga
     4801 atatcgtcat aatggagcta ataaagccaa atatagcagc gataagcata ctttttttag
     4861 ctataaaaat atgacgtttt ttaagtaaat accaagatga aacactgatg acaaatagag
     4921 atgccaccac ataactactc gtagtagtat gtaaaaattt attaatggct acaggactca
     4981 aggctatatc ccaaaaattt atcatttcat ttctagcggt agcaggatta aatactgtac
     5041 cgataggatg ttgcatccaa gcattagcta tcaagatcca aagagctgat agattagatc
     5101 ctatagctac caaccaggta gaaatcaaat gaagtttttt acttactcta tcccaaccaa
     5161 aaatcataac tgctataaaa gtagcttcca taaaaaaggc taaaatacct tctatagcta
     5221 gtggtgcacc aaaaatatcc cctacaaacc agctataatt actccaatta gttccaaact
     5281 caaacactaa tataatacct gtggctacac ccagtataaa gttaataatt aataatttca
     5341 tccaaaactt cgtcatcttt ttccactcat cattgtctgt cttataatag tgagtatgca
     5401 taatagctat cataatagta agccccagtg taatagggac aaaaatccaa tgatacatgg
     5461 cagttagagc aaattgacca cgtgaccagt tgactaagga tattaattcg tccatattat
     5521 ttgatttttg tggctaattg ttcgattaca tgatcagcta tatctttatc agtattataa
     5581 ttagttttta atatattagg gagaaatatt aatttgagaa taataaaaaa aacaaagaaa
     5641 ttgggaaata gtataactaa aagttgtctg ccagttttac ttatatttct aaaacattca
     5701 atatataagt aagtgatttt ttttagcatt agttttattt tttattaaat attttgagca
     5761 atttaaaaaa tttgagcccc actatcttgt agtcatttgt gattttagtt ttgagattta
     5821 tagagtaatg actggcactg aggcttaggt attatcaatc gagtgcttat cgaaatgaca
     5881 tttagcaagc atagtcaaaa tatttctgac tagaaatgag taacattaaa ctatttctat
     5941 tctcaaattt tactaataat tgcaacattt tatatttaat tttattaaaa atctattatt
     6001 ctacagtaac agatttggct aaatttcgtg gttgatctac attgcatcca cgcaaaactg
     6061 caatatgata agctaacaat tgtaagggga tggtggcaac tagaggtact agaaactctt
     6121 gagttttagg aatttcaata taataatcac tcaatttttt aacagtatca tcaccttcag
     6181 agactatcga aataataatg ccttttctag ctttaacttc ttgaatatta gatacagttt
     6241 tatcataaat attactttga ggtgctatga aaacgacagg catattttca tctattagag
     6301 ctatggggcc gtgtttcatt tcagcggcag ggtatccttc ggcgtgaata taacttattt
     6361 ctttcaattt cagagctcct tctaaagcta ctggatagga gtaacctcta ccaaggtata
     6421 agaaatttct tgcgtctttg aactgacttg caatatgctt aattggttca ttagtttttt
     6481 ctagagttat ttgcattttt tctgcaattt cgtttagatc gtggagatgt tgaataaatc
     6541 tagatttagg tatcacgcct tttttataac ctaacattag agccatcata gttaatatag
     6601 ttatctgagc agtaaaagct ttagtggatg ctactcctat ttctgggccg gcatgagtat
     6661 aagagcctgc tacggtctca cgtgctatag aagatcctac aacattgcat atacctaatg
     6721 gaatagctcc cttactttta gctaatttaa cagctgctaa agtatcggca gtttcaccag
     6781 actgagatat tgctatgaca acatcgtttt catgtaatac agggttgcga taacgaaatt
     6841 cagatgcata ctctacttcc actggtatac gtgccagatc ttctagcaaa tattcaccta
     6901 ctagaccggc atgccatgat gtgccacaac cgactaatat tattcgaggg gctgtcagta
     6961 atctgttttc atattctact attccaccta aggcaatggt ttgttttgct aaagaaagtc
     7021 tgccacgcat actatctttt acactttgtg gttgttcgaa aatttctttt aacataaaat
     7081 gttcgtaccc gcctttttct atttgagaaa gactcatttc taactgctgt atatatggag
     7141 ttttttccag atttttaata gtaatgattt taagatcctt atttttttgt attacagcta
     7201 tctcgtcatc ttcgagataa actactttat tagtatattc tataataggt gaggggtcag
     7261 atgcgacgaa atattcatca tcgcctactc cgatcactaa tggactacca cgtttagctg
     7321 ctatcagcat atcaggattg ttttctgata aaataacaat agcaaaggct cctatgactt
     7381 gagatagtgc taaacgcgtt gcttctacta tatctacatg ttcatttttt attatatctt
     7441 ctactaaatg aattattact tctgtatctg tttcactttt aaattgataa tcccgagata
     7501 ttaattcttc ttttaaggtt ttataatttt caataatacc attatgaatt atagctaatt
     7561 tatgcgaagg cgaaaaatga ggatgagcat ttatatctgt tggctctcca tgagtagccc
     7621 atcgtgtatg acccatacct acagtgccac taagatcttt gtctataaca caggcttcta
     7681 gatcagatac tttaccatga gttttatata tttttaaccc attatttaaa agtgaaatac
     7741 cagcactatc atagcctcta tattctaacc tgtgtaatcc attaataagt ataggaaaag
     7801 cttgcctata cccaatgtaa cctattatac cacacataat atattatata ttaatcaatt
     7861 ttttatttta tgtaaaaata ataatttttt taataaacaa aaaatacaat atcaatgtaa
     7921 agatatctat tacctgtttt ttttacaaag ttaatgaaaa aataaaaaat tgcttacaca
     7981 cgtaaaatta taatattttt ttcatatgct acatggataa tagagaaaac tgaagtcaaa
     8041 aaaattgaga aaataagtaa aaagtgtaac aaagttaaaa gtagataggt tttagagagt
     8101 gagatttttt ttatttatta ttttgttttt tataaatatt cattgttttt aattgtgtta
     8161 cttaatattt tatattataa aaattttagt ttaactttgc aaatataaat ttgttctatg
     8221 ttaaaaaata ataaatttaa tattttttta ataagtttag tacttttatg tttatgtagt
     8281 tgtcagaaaa aagaaaaaga atattttgag tcacttggta atcacaaatc atttttatat
     8341 attgatcata gggaagatac tatttatact ataaaaagaa gtaactatga tgtaaaaatt
     8401 tatttatttg cgaataaaaa tttttttact cgaggtacag tattgttatt acatggttgg
     8461 aatctatcac cattatcttg gtgcgatcaa acaaattttt gtgatgaatt actagctagc
     8521 ggttaccatg tgctgatacc agatttaggc aaaagtgtat atgctacgca atatttcaaa
     8581 gaaaccgtac ctagtatgac taaatatcct caaatgccat ttattttaga tactttgatt
     8641 aattttttag tagattatat taaaattatt gatacatctc attataattt tttagtaggt
     8701 tattcaatgg gtgcgagagg tgcattagca attgcagaga atacaaataa tctttttagt
     8761 gctttagttc ttttagctgg cgaatatgat caaacattga tgcccgacga taatattatg
     8821 actatgtatt atggttctta tcaaaaattt cctgatagat gggaaggcaa tgataatctt
     8881 ttacgcaata tagataaagt taatattcct gttctgatat tacatgggtt agatgatgat
     8941 gtcataccag tcgcccaatc agatacctta gctaaagttt tagaaaagca taataaattt
     9001 tttataaaaa tttattatta caattatggt catggatttg atttcgtaca aaaaactatt
     9061 aataatataa agtctttttt tgaaaaagtt atttatcctt ggaatttgga tgtatagact
     9121 agaattttat aaaattgaca aaaaaataca aaattaaaag atatctagtg tttctatatt
     9181 agaaatttaa tttatttcga acattatttt ttagataata tcataaaata agttaggtat
     9241 taaagtatat ttctgaatta tatatactta ttttgcttac tttgttaact acacaacaat
     9301 aagaaaagtg tctgtttagg aaatatgatg agagcaatag actgagatga gatgttttag
     9361 tttttttaaa aaggatttaa tcatcatcgt tgcaataatt gactaaaatt atttttaata
     9421 tagcaaattt ttaatatagc aaaataaatt taaaaattct tattatcttt gataaataat
     9481 ttttttatga attatgctta ttcataaaat ggccaataaa ttcatttgtc ctctttatta
     9541 tagaagagca ttaagtacta ttattatagc aagattatgt ctattgcaat taaaagctct
     9601 cgtaagtcat tattcatcaa ttaatattac ttgtaatttg tgtagtatag aataaagaga
     9661 aaaacacttc aaaaaataca gcgtaaatat gaataagtca gtaaaaactc gttcaaaatc
     9721 ccaaaaagaa aaaataaagc ttaataataa atcatggatt actaaaataa cagaatatcc
     9781 tgataaaaaa ttgtgctggt ttttattttt actagctact attatttatt tgccaactat
     9841 tacgtttgat tatgtattag acgatggcat tgtgatacat gaaaataaat ttacacaaaa
     9901 aggtataaaa ggtatcaaag aaatctttac ccatgatagt tttgtcggtt atttcggtga
     9961 agaggttaaa tatgtagctg gcggtcgcta tcgtccattt actcaattta tctttgctat
    10021 t
//
LOCUS       DAOPRN010000066         9839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_5771_length_9839_cov_109.146259, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000066 DAOPRN010000000
VERSION     DAOPRN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5771_length_9839_cov_109.146259"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..2212
                     /gene="ppk1"
                     /locus_tag="PKI65_07040"
     CDS             146..2212
                     /gene="ppk1"
                     /locus_tag="PKI65_07040"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOB78232.1"
                     /translation="MYNKNHFVNRELSWLDFNERVLQEAESLDNPLIERIRFLGIYSN
                     NLDEFYRVRYATLTRLVKVKGKKNKYSKYFKPQEIIEKINIRVKKLQERQDNIFENII
                     KELENHQIYLLRETDMTEQIHIDFITNYFNEILRPNIYPIMLHSLRPYGVFKDSSVYL
                     AVHMQYLLGTNKNKKKENFALIEIPDSIERIIVLPEINGSKYIMFVDDIVRYFLSKIF
                     KFLHYDHFTAYALKMTRDAELNIDNDISKSLMELIAEKIDERERGIPVRMVVDECIPD
                     KLLSLVLKKFNIDKKATIVKGQRYHNMRDLMEFPDLGLENLVYQPLEMIPHKTFSQSK
                     SLLETLKKRDQFISFPYQSFYNLLDILREASIDPKVKSIKITIYRTGRNSSVMNALIN
                     AARNGKKVTVFLELQARFDEENNIYWSNLLQKEGIKVIHSDPGIKVHCKLLLIKRLEN
                     EKLIYYAGLNTGNFNLYTSKVYTDFIILTRDSVIAEDVNNIFELLELNFKIPDFNKII
                     VSPFYMRDFVHHQLDNLINSCNNGYPANAIIKLNHIVDEEIIEHIYMAAKIGVKFNFI
                     IRTTCCLKPIYPTIKIRSIVGRFLEHARILYFDFAKNKDELYLSSADWMPRNMDHRIE
                     VTYPILDKNIKQVLIDILQLQLKDNVKARHVNNEFNNQIITNDYQTLNSQMAIYEYLK
                     QYFFNS"
     gene            2217..3110
                     /locus_tag="PKI65_07045"
     CDS             2217..3110
                     /locus_tag="PKI65_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78233.1"
                     /translation="METYAAIDIGSNAARLNISSTYIVNNNVFLQKILLLRLPLRLGE
                     TVYEKNIITSEKQKKLIKTINIFKDLLDYYEIKEVRAYATSAMREAKNKEKVINAVFK
                     KTNIFIKEITGQQEAKIIANLLKNYGEPHHTKLLIDVGGGSTQISIFYPDGSMINDSF
                     KIGTVRMLKNNIKAVEWNKMMDFVQKNTKKDNLFVIGSGGNINFIKKRFGSYYDPFLD
                     LSLLIDIYEQLQAFSIEERIINYSLNPDRADVIVPALIIYIKILQTLNIDKIACYQVG
                     LSDGIIMEQFYDKHPEFILNK"
     gene            complement(3292..3666)
                     /locus_tag="PKI65_07050"
     CDS             complement(3292..3666)
                     /locus_tag="PKI65_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78234.1"
                     /translation="MKKLNVFVLVVVLFIGFSSCVIEVKETKTKPMPPPHAPAHGVRA
                     KYHYRFYPSLNIYYDINRGIYFYLESGVWKSSNVLPGHISINLYDYVVIDLDTDTPYK
                     YYKKHKNKYHPNKNKPPKKPKK"
     gene            3808..4311
                     /gene="folK"
                     /locus_tag="PKI65_07055"
     CDS             3808..4311
                     /gene="folK"
                     /locus_tag="PKI65_07055"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOB78235.1"
                     /translation="MHTIYLSLGSNLNNRQENIFKAKVLIKKNIGIITKESSIYLSEP
                     WGYSSSNNFLNQCIAINTHLEPLELLRGLKKIETILGRKKQPNNIYIDRTIDIDILFY
                     DNIYYKYLDILTIPHPLLHTRRFVLLPLVEIAPNFFHPLLNQSISELLNKCKDKTLIL
                     NLDHINS"
     gene            4465..5001
                     /locus_tag="PKI65_07060"
     CDS             4465..5001
                     /locus_tag="PKI65_07060"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOB78236.1"
                     /translation="MKTKQLLNHASKYTLFAAAIMVIYLLLMWIVNANILKLNLISFL
                     IQAFAIIFFSLKAIEVARVEYGDGYISYFNAFLLCFIISFFSLLIFLLVKLLIFYVID
                     PGYLNNLVNSLIVELEMQKDMIPESFQSFATSENIALTYSFKYQLATAFNYLIESAIL
                     GLIIAGFARKKPSINENF"
     gene            5062..6003
                     /locus_tag="PKI65_07065"
     CDS             5062..6003
                     /locus_tag="PKI65_07065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOB78237.1"
                     /translation="MDISVVIPLYNEEESLAELTEWIRRVMRENNFSYEIIFVDDGSV
                     DGSWDEIIKLSENNNDIKAIRFRRNFGKSAALHVGFQQVEGDVVITMDADLQDSPDEI
                     PELYRMIKEDGYDLVSGWKKKRYDPLTKTIPTKIFNWGTRVVSGIKLHDFNCGLKAYK
                     HDVVKSIEIYGEMHRYIPIIAKWAGFNKIGEKIVHHYPRKYGTTKFGMSRFIRGPLDL
                     LTISFVTKFGKRPMHFFGTIGLVLFIIGFIIGLYLAIAKIMFQQYKMTERPLFYLGIL
                     FMILGTMLFIGGFLAELIQRANPDKNKYDIKEEINTL"
     gene            6024..7241
                     /locus_tag="PKI65_07070"
     CDS             6024..7241
                     /locus_tag="PKI65_07070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB78238.1"
                     /translation="MNKNWERVAMVGLAFPFKGGMAALKNKLAIELQKTGKDVEIFGF
                     KKLYPYFIFRKEEKITDKAQIEDFYNKYQLKIHNFLKSWNPNTWRFTAKLISSRKPDI
                     VIYQYWVAFLAVVYGKIAKYIKKNNSNIVEIAIVNSIYGKHKKIQKIFFKYFSRYIDG
                     FIFLSEKVAHEFNELNINKPYLIVHHPLYDQYGELIPKEKALNILNLPQKIFIDNEPV
                     NIKYILFFGQIKEYKGLDLLLQALANTDPSIHLIVAGDFFQSENVFKDIIDKLNLKDR
                     VAIFDKYIPDSEVPVFFSAADLVVMPYKVVKQSGITRIALRYNKPFITTDTDILDIIA
                     DKVCDTDFIITTDPMEISKAINTFFSDNPEDKIKAKKWVQAQNILANILSWEKMITAI
                     DELYKLNNKNVFL"
     gene            7333..8733
                     /gene="ahcY"
                     /locus_tag="PKI65_07075"
     CDS             7333..8733
                     /gene="ahcY"
                     /locus_tag="PKI65_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOB78239.1"
                     /translation="MEKIPYRVKDISLADWGRREIELAEKEMPGLMQLREKYSKDKPL
                     SGARISGSLHMTIETAVLIETLVQLGAQVRWASCNIFSTQDHAAAAIAKAGVNVYAWK
                     GETLHEYWWSTAQALNHNGLGAHLIVDDGGDATLMVHLGYKVEENPSILKNKPSSKDQ
                     QALYDILEEIYNEDANFWHKTVKDLKGISEETTTGVHRLYQLNERGELLVPAINVNDS
                     VTKSKFDNIYGCRESLLDGIKRATDRMIAGKTAIVCGYGEVGKGAAQALRGVGARVLI
                     SEIDPICALQAAMEGYEVTTVEDALSEATIFVTATGNKDVIRIEHMTKMKDNSIVCNI
                     GHFDNEIQVDELQNYPGIIHTNIKPQVDKYTFPDANSVILLAEGRLVNLGCATGHPSF
                     VMSNSFSNQVLAQLDLWQKDYKVGVYRLPKYLDEEVARLHLPFLGVKLTKLTEDQAEY
                     IGVPIDGPYKSEHYRY"
     gene            complement(9162..>9839)
                     /locus_tag="PKI65_07080"
     CDS             complement(9162..>9839)
                     /locus_tag="PKI65_07080"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78240.1"
                     /translation="DKLVESSFISNKTSEICYVFPLYLYNKTIPQKSNLKTMMVFEKH
                     ADYASKKPNFSPDFLKFITEKYGKTLTPEEIFYYIYAVLHSPSYREKYAEFLQYDFPR
                     IPFVDDYETFQKLSEIGKSLTELHLMKCNLPTRVKFDIPGSGIVEKVKYDNGKIWINK
                     VQYFDGIPEDIWCFYIGGYQVLDKWLKSRAKLKLDSESIMQFLQITEIIGETINLMNE
                     IDKIIEL"
BASE COUNT         3676 a         1271 c         1453 g         3439 t
ORIGIN      
        1 gagttagtaa aactaacata tgttgatttt aacatatgtt agttttccga attgattaat
       61 ttttcgattc tttcattcca acccccctcc cccaacaagc ccttttaata aaaaaattgt
      121 aattttgaaa ttaatttaaa ttaaaatgta taataaaaat cattttgtta atagagaatt
      181 aagttggtta gatttcaatg aacgtgtatt acaagaagct gagagcttgg ataatccatt
      241 aatagaacga attcgtttct tgggaattta ttcaaataac ttagatgaat tttatcgtgt
      301 acgttatgcg actttaactc gcttagtaaa agtaaaaggt aaaaaaaata aatattcaaa
      361 atatttcaag cctcaagaga taatagaaaa aataaatatt agagtaaaaa aattacaaga
      421 acgacaagat aatattttcg aaaatataat aaaagaactc gaaaatcatc aaatatactt
      481 attaagagaa actgatatga cagagcaaat acatattgat tttatcacta actactttaa
      541 tgaaatacta cgcccaaata tttaccctat aatgttgcat tctttgcgtc cttatggtgt
      601 cttcaaagat agttcagttt atctagctgt acatatgcaa tatttattag gaacaaataa
      661 aaataaaaaa aaggaaaatt tcgctttaat agagatacct gatagcatag aacgtataat
      721 agtattgcct gagatcaatg gtagtaaata cataatgttt gtagatgaca tagttagata
      781 ttttttatca aagattttta aattcctcca ttatgatcat ttcacagctt atgctttaaa
      841 gatgacaaga gacgccgaat tgaatataga taatgatata tcaaaatcgt taatggaact
      901 gatagcagaa aaaatagacg aaagagaacg tggaattccg gtacgtatgg tagtagatga
      961 atgtatccca gataaattat tatcattagt attgaaaaaa tttaatatag ataaaaaagc
     1021 tactatagta aaaggtcaac gatatcataa tatgagagat ttaatggaat ttcctgacct
     1081 aggattagag aatctcgttt atcaaccttt agaaatgata cctcacaaaa cttttagtca
     1141 gtccaagagc ttattagaaa cattaaaaaa aagagatcaa tttattagtt tcccgtatca
     1201 atctttttac aatttattag acattttacg tgaagcttct atagatccta aagtgaaaag
     1261 tatcaaaatt actatttata gaactgggag aaattcaagt gttatgaatg ctctgataaa
     1321 tgccgcacgt aatggtaaaa aagttacagt attcctagaa ttacaagcta gatttgatga
     1381 agaaaataat atatattggt ctaatttatt acaaaaagaa ggtatcaaag tgatacatag
     1441 tgatcctggt attaaagttc attgtaaact attattaatc aaaagattgg aaaatgaaaa
     1501 attgatttat tatgcaggat taaataccgg taattttaat ctttatactt caaaagttta
     1561 taccgatttt attattttaa ctcgtgattc tgttattgcc gaagatgtta ataatatttt
     1621 tgaattatta gaattaaatt ttaaaatacc cgattttaat aaaatcatag tctctccttt
     1681 ttacatgaga gattttgtac atcatcagct tgataatttg attaatagtt gtaataatgg
     1741 ttatcctgca aatgctataa taaaactaaa tcatattgta gatgaagaaa taatagaaca
     1801 tatctatatg gcagcaaaaa ttggtgtaaa atttaatttt attataagaa caacctgttg
     1861 tctgaagcct atttatccta caataaaaat tcgtagtatt gtaggtagat ttcttgagca
     1921 tgctagaatt ttatattttg attttgctaa aaataaagat gaactatatt tatctagtgc
     1981 tgactggatg ccacgtaata tggatcatcg aatagaagtt acatatccta ttttagataa
     2041 aaatattaaa caagttttaa tagatatttt gcaattacaa ttaaaagata atgtaaaagc
     2101 aaggcatgta aataatgaat ttaataatca gataattact aatgattatc aaacattaaa
     2161 ttctcaaatg gcaatttatg aatatttaaa acaatatttt tttaattctt agaattatgg
     2221 aaacatatgc agctattgat ataggatcta atgctgctag gttaaacatc agcagcacat
     2281 atatagtaaa taataatgtt tttttacaaa aaattttatt acttagacta ccactgagat
     2341 taggggaaac tgtttatgaa aaaaatataa taacaagtga aaaacaaaaa aaattgataa
     2401 aaactataaa tatttttaaa gatctactag attattatga gataaaagaa gtaagagctt
     2461 atgctaccag tgctatgcgt gaggcaaaaa ataaagaaaa agttattaat gcagtattta
     2521 aaaaaacaaa tatttttatc aaagaaatta ctggccagca agaagcaaaa attatagcaa
     2581 atttattaaa aaattatggt gaacctcatc acacaaaatt attaatagac gtcggtggtg
     2641 gaagcacaca aatatctatc ttttatcctg atggatctat gataaatgat tcttttaaaa
     2701 taggcactgt gagaatgctt aaaaataata taaaagcagt agaatggaac aaaatgatgg
     2761 attttgttca aaaaaatact aaaaaagata atttatttgt cataggttct ggtggtaata
     2821 ttaattttat aaaaaaacgt ttcggatctt attatgaccc ttttttagat ttatcattat
     2881 taatagacat ttatgaacaa ttgcaagctt ttagtattga agaaagaata attaattatt
     2941 ctctaaatcc agatagagca gacgtaatag tgccagcatt aataatatac ataaaaatct
     3001 tacaaactct aaatattgat aaaatagctt gctatcaagt aggtctttca gacgggataa
     3061 ttatggaaca attttatgat aaacacccag aatttatatt aaataaatga cattatttat
     3121 ttaatataaa ttttttcgtt gtagtttata cgctaatctg agttgagcac ctcatattat
     3181 tataccttac taattatttg taagttataa ttatttaatt tcttcttttg aaaattatac
     3241 ccctttaatc aatttattag atttatctaa tttacaaata gttttaccta attatttttt
     3301 aggttttttg ggaggtttat ttttattagg atgatattta tttttgtgct ttttataata
     3361 tttatagggt gtatctgtat ctaaatctat aacaacgtag tcgtataaat taatcgatat
     3421 atgcccaggc aatacattag aagatttcca tacaccagac tctaaataaa aataaatacc
     3481 tcggtttata tcataatata tattaaggga tggataaaaa cgataatgat atttagctct
     3541 cactccgtgt gcaggggcat gtggcggtgg catgggtttg gttttagttt cttttacctc
     3601 tattacacaa ctactaaatc ctatgaaaag gactacaact aaaacaaaaa catttaattt
     3661 tttcataata aatttattta ttatttatca aaattaataa ataaatttgt atttaattat
     3721 aatgccaatt acactatttt tttttaaaaa ttatttattt tgttatgtac aaaaaatatt
     3781 ttatataatt ttgtaaaaaa aaaatttgtg catactatat atttatcact aggcagcaat
     3841 ctcaataata gacaagaaaa catatttaaa gccaaagtcc ttattaaaaa aaatatcggt
     3901 attatcacaa aagaaagttc tatatattta tcagaaccat ggggctatag ttcgtcaaat
     3961 aattttttaa atcaatgtat agctattaat actcatttgg aaccactaga gcttttaaga
     4021 ggattgaaaa aaatagaaac tattttaggt agaaaaaaac agcctaacaa tatttacata
     4081 gataggacta tcgacattga tatacttttt tatgataata tttattataa atacttggat
     4141 atacttacta taccacatcc acttttacat acacgtagat ttgttctttt acctttagta
     4201 gagatagctc caaatttttt tcacccgtta ttaaatcaaa gtattagtga attattaaat
     4261 aaatgcaaag ataagacatt aatactcaat ttagaccata taaattcata gttcgacgac
     4321 atctaaacaa caaaatgaaa tatatttggg ctatatgatt agtaagtttt acaatttttt
     4381 tttacctttg aaataaattt tgctacacaa ttttattaaa aaatttaaaa gttttacaat
     4441 taattattat aaatttgcaa aaaaatgaaa acaaaacaat tattaaatca cgcatctaag
     4501 tatacacttt tcgcagcagc tatcatggta atatatttat tattaatgtg gatagtaaat
     4561 gctaatatct tgaaattaaa tcttattagc tttctaatac aagcttttgc tataatattt
     4621 tttagtttaa aagctatcga agtagcaaga gtagaatatg gtgatggcta tataagctat
     4681 ttcaatgcct ttttactttg ttttattatt tcctttttta gtttactgat tttcctatta
     4741 gtaaaattat taatatttta cgttattgac ccaggatatt tgaacaattt agtcaactcg
     4801 ctaattgtag agttagaaat gcaaaaagat atgattccag aaagttttca gtcatttgct
     4861 acttcagaaa atatagcttt aacgtattct tttaaatatc aattagctac tgcatttaat
     4921 tatctgatag aatctgctat tttaggtttg ataatagcag ggtttgctag aaaaaaacca
     4981 tcaattaatg aaaattttta aatataccat ttttgtaact ttaaattatt ttcatttttt
     5041 agatttaata attttttaat tatggatata tcagttgtaa taccattata taatgaagaa
     5101 gaatcattag cagagctcac tgaatggatt agaagggtga tgagagaaaa caatttctct
     5161 tacgaaatta ttttcgtaga tgatggtagc gttgatggct catgggacga aattataaaa
     5221 ttatctgaaa acaacaatga tataaaagct attagattta gacgaaattt tggtaaatca
     5281 gcggctctac atgtaggatt tcaacaagtt gaaggagatg ttgtgattac catggatgct
     5341 gatttgcaag atagtccaga tgagatccca gagttatata gaatgataaa agaagatggt
     5401 tacgatctgg tctctggctg gaaaaagaaa cgttatgatc cattgactaa aacaatacct
     5461 actaaaatat ttaattgggg tactcgagta gtctcaggaa tcaaattgca tgactttaac
     5521 tgtggattaa aagcttacaa gcatgatgta gttaaaagta tagaaattta tggagagatg
     5581 catagatata tccctattat agctaaatgg gctggattta ataaaatagg agaaaaaata
     5641 gtacatcatt accctcgtaa gtatggaact actaaatttg gtatgtctcg atttatacgt
     5701 ggtccattag atttacttac aatttctttt gttacaaaat ttggaaaaag acctatgcat
     5761 tttttcggta ctattggatt agttttattt atcattggtt ttatcattgg tttatattta
     5821 gctattgcta aaataatgtt tcaacagtat aagatgaccg aaagacctct cttttattta
     5881 ggtattttat ttatgatact aggtactatg ctttttatag gtggattttt agctgaatta
     5941 atacaaagag ctaatccaga taaaaataaa tatgatatca aagaagaaat aaatacactt
     6001 taaaaaaaat aatataaaat tttatgaata aaaattggga acgggtggct atggtaggct
     6061 tagcttttcc gtttaaaggt ggaatggcag ctctaaaaaa taaattagca atagaattac
     6121 aaaaaacagg taaagatgtt gaaatttttg gattcaaaaa attatatcct tattttattt
     6181 ttagaaaaga agaaaaaatt actgataaag ctcaaataga agatttttat aataaatatc
     6241 aattaaaaat tcataatttt ttaaaatctt ggaatcccaa tacttggcgt ttcactgcta
     6301 aattgatttc atctcgaaag ccagatattg ttatttatca atattgggtc gcttttttag
     6361 ctgttgtata tggaaaaatt gctaaatata ttaaaaagaa taatagcaac attgtagaaa
     6421 tagctatcgt taatagtatt tatggcaaac ataaaaaaat tcaaaaaata ttttttaaat
     6481 atttttcaag atatatcgat ggatttattt ttttatcaga aaaggtagct catgaattta
     6541 atgaactaaa tattaataaa ccatatctca tagtccatca tccattatat gatcaatatg
     6601 gcgagctgat acctaaagaa aaagctctaa atatattgaa tctaccacaa aaaatattta
     6661 tcgacaatga gccggtaaat attaaatata ttttattttt tggccaaata aaagagtata
     6721 aaggattaga tttattgctc caagccctcg caaatacaga tccttcaata catctaatag
     6781 tagctggaga tttctttcaa tcagagaatg tctttaaaga tattatagac aaattaaatt
     6841 taaaagatag agtggcgatt tttgacaaat acatccctga tagcgaggta cctgtatttt
     6901 tttcagcagc agatctagta gtaatgcctt ataaagttgt caaacagagt ggtattactc
     6961 gaatcgctct cagatataat aaaccattta taactacaga taccgatatt ttagatatta
     7021 tagctgataa agtttgcgat actgatttta ttattactac cgatcctatg gaaatttcta
     7081 aagcaataaa tacgttcttt agtgataatc ctgaagataa aattaaagca aaaaaatggg
     7141 tacaagctca aaatatttta gcaaatattt tatcttggga aaaaatgatc acagccatag
     7201 acgaattata caaattaaat aacaaaaacg tttttttata aagtttgttt ttacaataag
     7261 tttattattc ctgacttcga caatgcatct aaatacatat tttttattaa ttttgcataa
     7321 aattaaattt aaatggaaaa aataccatac agagtaaagg acatctcttt agccgattgg
     7381 ggacgacgcg aaatagagct agcagaaaaa gagatgcctg gattaatgca attgagagaa
     7441 aaatatagta aagataaacc attaagtggt gctcgaatct ctggttcatt gcacatgact
     7501 attgaaacag cagtacttat agaaacattg gtacaattag gtgcacaggt aaggtgggct
     7561 agctgtaata ttttctctac tcaggaccat gctgccgctg ctattgctaa agctggcgta
     7621 aatgtatatg cttggaaagg tgaaacactc cacgaatatt ggtggtctac agcacaagct
     7681 ctcaatcata atggtttagg ggcccattta attgtggatg atggcggtga tgctactcta
     7741 atggtacacc tcggttataa agtggaagaa aatccttcta tactcaaaaa taaaccctct
     7801 tctaaagatc aacaagctct atatgatatt ttggaagaaa tatataatga agatgctaat
     7861 ttttggcata aaacagtgaa agatcttaaa ggtatttctg aagaaaccac tacaggtgtt
     7921 catagattat atcaattaaa tgaacgtggc gagctattag taccggctat aaatgtaaat
     7981 gatagcgtta caaaatctaa atttgataat atttatggtt gcagagaatc tctgctagat
     8041 ggtattaaaa gagccacaga tcgtatgata gctggcaaaa cagctatagt ttgtggttat
     8101 ggcgaggtag gcaaaggtgc tgcgcaagct cttagaggcg taggtgctag agtattaatc
     8161 agtgagatag atcctatttg tgctctgcaa gctgctatgg aaggctacga ggttactacg
     8221 gtagaagatg ctcttagtga agccacaatt ttcgttaccg ctactggtaa caaagatgta
     8281 atacgcatag aacacatgac aaaaatgaaa gataatagca tcgtctgcaa tattggacat
     8341 tttgacaatg aaatccaagt tgatgaattg caaaattacc caggcattat tcatacaaac
     8401 attaaacctc aagtagataa atatactttc ccagatgcca actctgtaat actattagcc
     8461 gaaggacgtt tggtaaactt aggttgtgct actgggcatc catcatttgt aatgagtaat
     8521 tctttttcta atcaggtact agctcaatta gatctatggc aaaaagatta taaagtagga
     8581 gtttatcggt tacctaaata tctagacgag gaagttgcta gattgcatct gccattttta
     8641 ggtgtcaaac ttactaaatt gacagaggat caagctgaat atattggtgt acctattgat
     8701 ggaccttata agtcagagca ttacagatat tgaaaaatat caacagatga tctataaata
     8761 tttgatagtt taaatatctt ttactttttt ccttattaat aattacattt attacatgaa
     8821 tttagaattt tttttatttt gttaattata tttggtactt atttaataac ttttgatgta
     8881 atcttttaaa aaaattataa ttttatcgcc agattttgtt taattgctca taaacattaa
     8941 aaactattgg acatactaaa tgtttttcta ttatcattat cagtctccat gcgaaattat
     9001 ccgtttgcaa atacggattt ctacagagcg tcgcggacga tgatcataat atatgcattt
     9061 attttctttt aataaatgac ctaaattcct aattttcatc ttctctttct tttgatagtt
     9121 gcttgatttt attgagatct gagatcataa atgaaatttt ttcacaactc tattatttta
     9181 tctatctcat tcatcagatt tattgtttct cctattattt ctgtgatttg caaaaactgc
     9241 attattgatt cactatccaa ttttagtttt gctctacttt ttagccattt atctagcact
     9301 tgatacccac caatgtaaaa acaccagata tcttcgggta taccatcaaa gtattggacc
     9361 ttattaatcc atattttccc gttatcatat ttcacttttt ctactatacc agaaccaggg
     9421 atatcaaatt taactcgagt aggcaaatta catttcatca aatgtagttc tgtaaggctt
     9481 tttccaattt cagataattt ttgaaatgtc tcataatcat ctacaaaagg tattctagga
     9541 aaatcatatt gtaaaaattc agcatatttt tctctgtaag atggtgaatg tagcactgcg
     9601 tagatgtaat aaaaaatttc ttctggtgtc agggttttgc catatttttc tgtaataaat
     9661 ttcaaaaaat caggactaaa gttgggcttt tttgatgcat aatcagcatg tttttcaaaa
     9721 accatcatag tttttagatt agatttttga ggaatagttt tattatacaa atacaaaggg
     9781 aaaacatagc aaatttcgct agttttatta gaaataaagc tactttcgac caatttatc
//
LOCUS       DAOPRN010000067         9554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_6001_length_9554_cov_103.999158, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000067 DAOPRN010000000
VERSION     DAOPRN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6001_length_9554_cov_103.999158"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PKI65_07085"
     CDS             complement(<1..577)
                     /locus_tag="PKI65_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOB78241.1"
                     /translation="METRKDVRELIIDAAEKFGNKTAFVYNDQEYSFIDLKDSVFRLA
                     NSLLDMGYKKGDKIAIYLPNCLEYVYSYYAIYSLGLVAVPIDFYFNPQEVFSIGRHCE
                     LKGIITTTNAKFNLLELKQSIPSLRDIITIEDNPKYLSFWKLASNSRADLEDQGIYIN
                     MMSSIFYTSGTTGMPKGATWNYEHIHLGAEQL"
     gene            complement(811..1587)
                     /locus_tag="PKI65_07090"
     CDS             complement(811..1587)
                     /locus_tag="PKI65_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOB78242.1"
                     /translation="MNVSILTYNVNGIRAAIRKGLIDFLRDDNSDIVCFQELKANADQ
                     IPDLLFHELGYQTYWHPAEKKGYSGTGLLTRIKPDKVELGMGIPKYDAEGRMIKAYYG
                     STVLINVYHPSGSSGEARQAFKMQWLEDFENYILEFRKQHPDLLICGDFNICHTEIDI
                     HDPIGNANNSGFLPEERAWIDQFIKKDFIDAFRFFHPNEPGHYTWWSYRAKNARERNI
                     GWRIDYFMVSKSLKDRLISCEILREAKHSDHCPVKLYFDL"
     gene            1972..2044
                     /locus_tag="PKI65_07095"
     tRNA            1972..2044
                     /locus_tag="PKI65_07095"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2005..2007,aa:Lys,seq:ctt)
     gene            2136..2810
                     /locus_tag="PKI65_07100"
     CDS             2136..2810
                     /locus_tag="PKI65_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOB78243.1"
                     /translation="MGVKENLQKLKEEIPSNVTIVAVSKTKSIDEIMQAYDAEHRDFG
                     ENYVQELRAKQPLLPPDIRWHYIGTLQTKQIKYITPFIHLIHSVTSIKHLEEINKRAK
                     QNDRIIPVLIELHIADETTKSGVDEDELWEILDFYSKNQLPNVEIQGLMGMATLTDDQ
                     DKIRNEFKKLKILFDKSKEKYFYDKSNFKHISMGMSGDYKLAIEQGSTIIRIGTLIFG
                     EREYKK"
     gene            2860..2947
                     /locus_tag="PKI65_07105"
     tRNA            2860..2947
                     /locus_tag="PKI65_07105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2894..2896,aa:Ser,seq:gga)
     gene            2991..3815
                     /locus_tag="PKI65_07110"
     CDS             2991..3815
                     /locus_tag="PKI65_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOB78244.1"
                     /translation="MKKIATVLVILTVSFGISVFAQDKVVQNENDTQQVQTEAVDTAA
                     APTLAAIDQEQQEEAEAKPTFHQVLKQKFIEGGVGWMTPILLCLILGLAISIERIIYL
                     SLARTNSKALLEQIEKLVLEQKDIEKAKELCRNTRGPIASVFYQGLLRFNESLENIEK
                     AIASYGSVQMGHLESNLTWLSLFIAIAPSLGFLGTVVGMVSAFDAIEAAGDISPTIVA
                     GGMKVALLTTVFGLITALILQVFFNYIVSQIETIVNDMEDSTITFMDIISMAKNNQ"
     gene            3818..4222
                     /locus_tag="PKI65_07115"
     CDS             3818..4222
                     /locus_tag="PKI65_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78245.1"
                     /translation="MKNKKIYNIIFAAVLIISTAITLFAKSIDVRIYVGYVLIILSLI
                     ALLYSAISVISKNIKKNLKSILAFLAAIALAVVFYFIAPTDDLSIAVYEKTNTPIGWG
                     KIVSAGMYLVYTLVGIVILALIYSQVRRLVKK"
     gene            4235..4807
                     /locus_tag="PKI65_07120"
     CDS             4235..4807
                     /locus_tag="PKI65_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOB78246.1"
                     /translation="MARKVPQLNMSSTADISFLLLTFFLLTSSINTDKGLSRMLPPLP
                     DPNAKPPVIKERNIFKVLVNTYDQLLVEGQPGDIRNLRKQVKEFIANPYNDPKLSEGE
                     IINLPHLGPVRKSKGVISLQNDRGTSYDMYIKVQNELAGAIYELRDELAKEKLGRSIY
                     QISDETILDEIQKAIPMAISEAEPKAVGGK"
     gene            4809..5279
                     /locus_tag="PKI65_07125"
     CDS             4809..5279
                     /locus_tag="PKI65_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOB78247.1"
                     /translation="MARFRKEKVSETPGLNTSSMSDIIFMFLFFFMVITTIREATLFV
                     RVTVPAATEVQKLERKNLVSYIYIGIPLRPEIYGSEPRIQLNDQFGTVEQISEFIEME
                     RAARPEQDRPFLTTSLKVDKDVRMGIVTDVKQELRKVGAYKINYSTRKVHSIID"
     gene            5377..7416
                     /locus_tag="PKI65_07130"
     CDS             5377..7416
                     /locus_tag="PKI65_07130"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78248.1"
                     /translation="MKRKYLLCTLVIIILTISIILIFFFRNKKTINENPYNYIPSETQ
                     LVFYISNFDKKISDITKSMPFKILKNNEDKIISEISSGLEALDSINEDMLKYINKSDL
                     YIAVSKNFPSIAPLYIVTFNDNKLSNKYLENLIQKMDKTLKKEKDFYTISLNSQNVIY
                     LKKINNIILFTKSKINFKADSNTINSIVDINKYIKPSQMISLVCIPDTDQNNLLVNLF
                     TPEIYKYNFLIINIPSAEAINEYEVIANPKNKKFISHLINKPLAGIKPSLIQYIPSLM
                     PIINIWSAKNIFSNVIKSYLENNNLKNKWDSLTYLSPIRIDETLFTWLGDNICMAHDK
                     KNNEIAMIINVKDKNKAKRNLMQWSRYAIKKPVYLGPFIFYPVKWGNIINFFFSDYII
                     APDIMYAFLSGEYLLIANTTEFLEKMIATKKFCTYNANINLSQDCFQMIYQPLKIEIN
                     NSVEMKYLKNLKTDKPTLIYLNNFQLLDTNIRFSCKIIWDSVGMQKAILEKQLFDNEQ
                     IYLLEWDSIIAMKDEEWSRINVSQVVQMSYSLDRYGPYLRLKAEGRINEKGQRTGLWR
                     YYYPNTNLWASISYIADKPDGNAFLYYNVANKKIKAIANFSKGELNGRYQEFYPNGAK
                     KNILSFTSNNRNGVAKYYDKNGNLIIEGNYKDNKKIGTWHFYTTYQELIPWQLEY"
     gene            7482..8825
                     /locus_tag="PKI65_07135"
     CDS             7482..8825
                     /locus_tag="PKI65_07135"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOB78249.1"
                     /translation="MGKNISVDLALANELFSEKDIEDFNPFLIHSQKNIIERSGTGNE
                     FLGWVDLPYIMLNDKKLFQELEELKNEIQKNNTDVIVMIGIGGSYMGARAIIEAMQYY
                     LPYKLLPIIYLGHHLESAYIERQLKLLENKNYYTIVISKSGTTTEPAIAFRLVREQMK
                     KKYGDAYSQRVITITDQKKGALRKLTNQEGYRSFIVPDDVGGRYSVLSPVGLVPLYLV
                     GIDIRELLTGAYDESKLVKIDCWQDNSAFQYVTMRHLAYQKGKPIELFTSFYPCLFYV
                     AEWWKQLFGESEGKNNLGILPTSAMFTTDLHSLGQYIQEGLRIFFETIISIDNPFNTL
                     TIPSSKDVDDGMEFLAGKQISEINKIAEKATAIAHSDGGVPVIKISIPHISTYYLGAL
                     LYFFEYSCALSGYCFQINPFDQPGVEAYKNNMYALLGKPGYEELARKLKKRIEKI"
     gene            8932..9312
                     /gene="umuD"
                     /locus_tag="PKI65_07140"
     CDS             8932..9312
                     /gene="umuD"
                     /locus_tag="PKI65_07140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOB78250.1"
                     /translation="MLLPYTEAISAGFPSPAEEYLELSLDLNKELIKHPASTFFGRVK
                     GNSMQDAGIFDGDILIIDKSLSPFDGAKAVCYIDGEFTLKTLKKDNENLWLLPANESY
                     KPIKITEENDFIVWGIVTYVIHKI"
BASE COUNT         3548 a         1274 c         1498 g         3234 t
ORIGIN      
        1 ttagttgttc tgcacccaaa tgtatatgtt cataattcca tgtagcacct ttaggcatac
       61 cagtagtgcc agaagtataa aatatgctcg acatcatatt gatatatatt ccttgatctt
      121 ctaagtcggc tctagagttg cttgccaatt tccaaaaaga taaatatttg ggattatctt
      181 ctatagtgat tatatcgcgt agtgagggta tactttgttt tagttcgagt aaattaaatt
      241 tagcattagt agtagtgatg attcccttta gctcacagtg cctgccaata gaaaatactt
      301 cctgcggatt aaagtaaaaa tctatcggta cagctactaa tccaagagag taaatagcat
      361 agtatgaata gacatattcg aggcaatttg gtagatagat agctatttta tcgccttttt
      421 tatagcccat atctaatagg ctattagcta agcgaaagac actatccttc aagtctataa
      481 aactatactc ttgatcatta tacacaaatg cagttttatt tccaaatttt tctgctgcat
      541 cgataattaa ttctcttaca tctttacgtg tttccatatt ttttgttttt agcaaatgta
      601 ataaattatt tgctttatat aacgaataat tattaaaatt attttattta tgtttttttg
      661 aaatcaatca acaggatttt atttatgttt ttttgaaatc aatcaacagg cctcatctaa
      721 tgctttattt attaagagat cttataataa tatcaatttt ctaatatttt gataaagaat
      781 ttgtatttat taaatgctat ttaaaaatta ttataaatca aaatataatt ttacagggca
      841 atggtcactg tgttttgctt ctctcaatat ttcacatgaa atcaatctat cttttaatga
      901 tttgctaacc ataaaatagt ctatgcgcca accaatattt ctctcgcgtg catttttggc
      961 tctatagctc caccaagtat aatgtcctgg ttcatttgga tgaaaaaagc gaaatgcatc
     1021 aatgaaatct ttttttatga attgatctat ccatgctcgc tcctcgggta gaaatccgct
     1081 attattagca ttacctatag ggtcatgaat atctatttct gtatgacaga tattgaaatc
     1141 gccacaaatt aataaatcag gatgttgttt tcgaaattcc agaatataat tttcgaaatc
     1201 ttctaaccat tgcattttaa aggcttgtct cgcttcgcca ctactaccac taggatgata
     1261 gacatttatc aaaaccgtag aaccataata tgcttttatc atgcgccctt cagcatcgta
     1321 tttaggtatt cccataccta attctacttt atcaggtttg atccttgtta atagtccagt
     1381 accagagtaa ccttttttct ctgcaggatg ccaataagtt tgatagccaa gctcatgaaa
     1441 taataaatct ggtatctgat cggcattagc ttttaattct tgaaagcata caatatcgct
     1501 attatcatct ctcaaaaaat ctattaaccc ttttctaatg gctgctctaa taccatttac
     1561 attgtatgtc aaaatagaaa cattcatatt tttattgatt tttgtaatta tttatccaaa
     1621 aataaataat ttgtttgaag tattataatt aaaaaataat tataacatta tttaaattat
     1681 gatttgtggt aggattgcta aaaaatatct caaagtaaag atatgcccaa atagcaaaaa
     1741 aatgtgaatt tttgagtttt ttagtttaca aaatttgata aaattattat aaaaaatcaa
     1801 aaaatatttt ttaggttatt ttctatttaa aaaatttcac ttttattttt attcacggat
     1861 tcaggtctat acttattgaa acactatttt cattatttac tacattgatt gtaaaatcaa
     1921 tattttacta atttttttta ttttaatttt tattgtaatt ttgcatttat actctccgta
     1981 gctcaggtgg tagagcagct gactcttaat cagcgggtcg caggttcgaa tcctgtcggg
     2041 gagacttgtg aaatgtgaag caaatttcac tttttttatt ttaatttttc aaaaaaaaca
     2101 actaattttg tgaaaggatt tttttaaaaa aaattatggg agttaaagag aacttgcaaa
     2161 agctaaaaga agaaatccca tctaatgtaa cgatagtagc tgtatctaaa accaaatcaa
     2221 tagatgagat aatgcaagct tatgatgctg agcatagaga ttttggcgaa aactatgttc
     2281 aggagttgag ggccaagcaa cctcttttgc caccagatat tagatggcat tacattggta
     2341 ctttacagac aaagcaaatt aaatatatca ccccttttat acatttaata catagtgtaa
     2401 catctattaa acatttagaa gaaataaaca aaagagctaa gcaaaatgac aggatcattc
     2461 ctgttttaat tgaattacat atagctgatg agaccactaa gtctggcgta gatgaggatg
     2521 aattatggga aattttggat ttttatagta aaaatcaatt gcctaacgta gaaattcaag
     2581 gtttaatggg tatggctaca ctgacagatg accaagataa aattagaaat gaatttaaaa
     2641 aattaaaaat attatttgat aaatcaaaag aaaaatattt ttatgataaa tcaaatttta
     2701 aacatatatc tatgggaatg tctggagatt ataaattagc aatagagcaa ggcagtacta
     2761 taataaggat agggactttg atatttggcg aaagagaata taaaaaataa tttttttgtt
     2821 aataaaaata gttttattat ttttgcaaat aaaaaaatgg gagagatgct cgagaggttt
     2881 aagaggcacg cctggagagc gtgtaatcgt ctaaagcggt tcgtgggttc gaatcccact
     2941 ctctccgtaa tggaaaaaga aaaacaaacc aaaaataaaa taatacaaat atgaaaaaga
     3001 ttgcaacagt attggtaatt ttaactgtgt catttggtat atccgtattt gcacaggata
     3061 aagtagtaca aaatgaaaat gatacacaac aagtacaaac agaagctgta gatacagctg
     3121 cagcacctac attagcagca attgatcaag aacaacaaga agaagcagag gcaaaaccaa
     3181 cctttcacca agtactaaaa cagaaattta ttgaaggtgg tgtcggttgg atgaccccta
     3241 tattgctctg tttgatatta ggattagcta tcagcattga gcgtataatt tatctttcat
     3301 tagctaggac taattcaaaa gctttattag agcaaattga aaaattggta ttagagcaaa
     3361 aagatataga aaaagcaaaa gaactatgtc gcaatactag agggcctatt gctagtgttt
     3421 tttatcaagg gttattaaga ttcaatgagt cattagaaaa tattgaaaaa gctatcgcat
     3481 cttatggtag tgtgcaaatg ggtcatttag agtctaatct cacttggtta tcattattta
     3541 tcgctattgc tccatcatta ggattcttag gaacagttgt aggtatggtt tcggcatttg
     3601 atgctatcga agcagcaggt gatatcagtc ctactattgt ggcaggtggt atgaaggtag
     3661 ctcttttaac aacagtattt ggactaataa ccgctcttat tttgcaagta ttctttaatt
     3721 atattgtttc acaaatagaa actatcgtaa atgatatgga agatagtacc attaccttta
     3781 tggatatcat ttcaatggca aaaaataatc aataattatg aaaaataaaa aaatatataa
     3841 cataatattc gcagctgtat taataatatc tacagctata acattatttg caaaaagtat
     3901 agatgtccga atttatgtag gatatgtatt gataatttta tcattaatag ctttgttata
     3961 ttcagctatt agtgtaatat ctaaaaatat aaagaaaaac ttgaagtcta ttttagcttt
     4021 tcttgcagct attgctttag cagtagtatt ctattttatt gctccaactg atgaccttag
     4081 tatagcagtt tacgaaaaaa caaatacacc aattggttgg ggtaaaatag taagtgcagg
     4141 catgtatctg gtatatactt tagtaggaat tgtgatttta gctctcatat actctcaagt
     4201 tagacgttta gtaaaaaaat aaaaaagtta aattatggct agaaaagttc cccaattaaa
     4261 tatgtcgtcg acagctgata tttctttctt attattgact ttctttttac ttacttcatc
     4321 tattaataca gataaggggt tgtctcgtat gctgccaccc ttgcctgatc ctaatgctaa
     4381 accacctgtt ataaaagaaa gaaatatttt caaggtactg gtcaatactt atgatcagtt
     4441 actagtggaa ggacagccag gcgatattcg aaatctgcga aagcaggtaa aagagtttat
     4501 agcaaatcct tataatgatc ctaaactaag tgaaggagaa ataataaatt tacctcactt
     4561 aggacctgta aggaaatcaa aaggtgtaat atcattgcaa aatgatagag gcacctctta
     4621 tgatatgtat attaaggttc agaatgaatt ggcaggtgct atttatgaac tcagagatga
     4681 attagctaag gaaaaacttg gtagatctat ttatcaaata agtgatgaga caattttaga
     4741 tgaaatacaa aaagctattc ctatggctat ttctgaggct gaacccaaag cagtaggagg
     4801 taaatgatat ggcaagattt agaaaagaaa aagttagtga aacaccagga ctcaatacct
     4861 cgtcaatgtc tgatattatt tttatgtttt tatttttctt tatggttatt actactatac
     4921 gagaggccac tctttttgtt cgtgtgactg taccagctgc tactgaagtg caaaaacttg
     4981 aaagaaaaaa tcttgtttct tatatctaca ttggtattcc attaagacct gaaatatatg
     5041 gatctgaacc acgtatacaa cttaatgatc aatttggaac ggtagaacaa attagtgagt
     5101 ttatagaaat ggaacgtgca gctcgtcctg agcaagatag accttttcta acaacttcac
     5161 tcaaagtaga taaagatgtg cgtatgggta tagtaactga tgtcaagcaa gaacttagaa
     5221 aagtaggtgc ttataaaatt aattattcta ctagaaaagt tcactcaatc atagactaaa
     5281 aatttatttg aattttattg gtaaaaggct gatatttttt gcaaaaagta tcagcctttt
     5341 gtatttataa agatattatt aatttagttg aaaaaagtga aacgtaaata tttattatgt
     5401 actttagtaa taattatatt gactatatcg attattttaa ttttcttttt tagaaataaa
     5461 aaaactatta atgaaaatcc atacaattac ataccatcag agactcaatt agttttttat
     5521 atatcaaatt ttgataaaaa aatctctgat attactaaat ctatgccttt taaaatttta
     5581 aaaaataatg aagataaaat catatccgaa atatcatcag ggttagaagc attggattct
     5641 ataaatgaag atatgctaaa atatataaat aaatcagatt tgtatattgc agtttctaaa
     5701 aattttccat cgatagcacc tttgtatatt gttactttta atgataataa actttcaaat
     5761 aaatatttag aaaatcttat ccaaaagatg gataaaactc ttaaaaaaga aaaagatttt
     5821 tataccattt cgctaaatag tcaaaatgtt atttatctta aaaaaataaa taacataata
     5881 ctttttacta aatctaaaat taattttaaa gctgattcaa atacgataaa cagcatagtt
     5941 gatattaata aatatataaa accttctcaa atgataagtt tagtttgcat accagatact
     6001 gatcaaaata atttattagt aaatttattt actcctgaaa tttataaata taatttttta
     6061 ataattaata tacctagtgc tgaagctata aatgaatatg aagtaatagc aaatcctaaa
     6121 aataaaaaat ttatttctca tttaataaat aaaccattag cagggataaa accaagctta
     6181 atccagtata taccatcatt aatgcctatt attaatatat ggtctgcaaa aaatattttt
     6241 agtaatgtaa taaaaagtta tttagaaaat aataatctaa aaaataaatg ggattctctc
     6301 acttatttat caccaatcag aatagatgaa acattattta cgtggctagg tgataatata
     6361 tgtatggcac atgataaaaa aaataatgaa atagcaatga taattaatgt aaaagataaa
     6421 aataaagcca agagaaatct aatgcaatgg tcacgttatg ctataaaaaa acctgtatat
     6481 ttaggtcctt ttatttttta tccagtcaaa tgggggaata tcataaattt tttcttctcc
     6541 gattatataa ttgcaccgga tataatgtat gcttttttga gtggagaata tttattgata
     6601 gctaatacta cagaattttt agaaaaaatg attgcaacaa aaaaattttg tacctataat
     6661 gctaatatca atctatctca agattgtttt cagatgattt atcaaccatt aaaaatagaa
     6721 ataaataata gtgtagaaat gaaatattta aaaaacttaa aaactgataa accaactctg
     6781 atatacttga ataattttca attattagat actaatataa gatttagttg taaaattatt
     6841 tgggatagtg taggtatgca aaaggctata ttagaaaagc aattattcga caacgaacaa
     6901 atttatcttt tagaatggga tagtataata gcaatgaaag atgaagaatg gagtcgaata
     6961 aatgtttctc aggtagtaca aatgagctat tcattggatc ggtatggacc ttatttaaga
     7021 ttaaaagcag agggcaggat aaatgaaaaa ggtcaacgta cagggctctg gcgttactat
     7081 tatccaaata ctaatctatg ggcaagtatc tcatatattg ccgacaaacc tgatggtaat
     7141 gcttttttat attataatgt ggctaataaa aaaataaaag ctatagctaa tttctctaaa
     7201 ggcgaactta atggtagata tcaggaattt tatcctaatg gtgccaaaaa aaatatttta
     7261 tcatttacta gtaataatag aaatggtgtg gccaaatatt atgataaaaa tgggaatcta
     7321 ataatagaag gtaattataa agataataaa aagattggca catggcactt ttatacaaca
     7381 taccaagaac ttatcccgtg gcaattagaa tattaggtag aaacacattg caatagtcta
     7441 tgtaaataga taaaattttt atgtaaattt gaaaaaaaat tatgggtaaa aatattagtg
     7501 tagatttagc tttagctaat gaactattta gtgaaaaaga cattgaagat tttaacccct
     7561 ttttaataca ttctcaaaaa aatattattg aaagatcggg aactggcaat gaatttcttg
     7621 gttgggtaga tttaccatat attatgctca atgataaaaa attattccaa gaattagaag
     7681 aattaaaaaa tgaaatccaa aaaaacaata cagatgttat agtaatgata ggtatcggtg
     7741 gatcatatat gggtgccaga gctattatag aagctatgca atactactta ccttataaat
     7801 tattgccaat tatttattta gggcatcatt tagaatctgc atacatagag agacaattaa
     7861 aacttttaga aaataaaaat tattatacaa ttgtaatatc taagagtggt actactactg
     7921 agccagctat agcttttaga cttgtcagag agcaaatgaa aaagaaatat ggcgatgcct
     7981 atagtcaaag ggtgatcact atcacagatc aaaaaaaagg tgctttacgt aaattaacta
     8041 atcaagaggg atatcgaagc tttatagtgc cagatgatgt aggaggtaga tattcagtgt
     8101 tatcccctgt gggtcttgtc ccattatatc ttgttgggat agacattaga gaattattaa
     8161 caggtgccta tgacgagagt aaattggtaa aaattgattg ctggcaagat aattcagcat
     8221 ttcaatatgt aactatgcga catctagcat atcaaaaagg caaaccaatc gaactgttta
     8281 cgtcatttta tccttgctta ttttatgtag ctgagtggtg gaagcaacta ttcggcgaga
     8341 gtgaaggaaa aaacaatctc ggtatattgc ccacctcggc tatgtttact acagatttgc
     8401 actcattagg gcaatatatt caagagggac tacgtatttt ttttgaaact attatctcca
     8461 ttgacaatcc atttaatact ttaactatac ctagttctaa agatgtagat gatggtatgg
     8521 aatttctggc tggcaagcag ataagcgaaa taaacaaaat agcggagaag gctacagcta
     8581 tagctcatag tgatggtggc gtgccagtga ttaagatttc tatccctcat atatctactt
     8641 attatttagg tgctttgctt tatttctttg aatattcttg tgcattaagt ggttattgtt
     8701 ttcagatcaa tccttttgat cagcctggtg tagaagctta taaaaataat atgtatgctc
     8761 tactaggaaa accaggttat gaagaattag ctagaaaatt aaaaaaaaga attgagaaaa
     8821 tataaaactc aaaaaatgtg tttattgttt taatatttat tttttatgaa gtctaagtct
     8881 aaaaaatcag atcaaaatat cgatctatat aattctaatt ttgaattaga aatgttatta
     8941 ccttacacag aagctataag tgctggcttc ccatcaccag cagaggaata tttagaatta
     9001 agcttagatt taaataaaga attaataaaa catccagctt ctactttttt tggaagggta
     9061 aaaggaaatt caatgcaaga tgcaggtatc tttgatggtg atattttgat aatagataaa
     9121 tcattaagtc cttttgatgg tgcaaaagca gtttgttata ttgatggaga atttacatta
     9181 aaaactttaa aaaaagacaa tgaaaatctc tggcttttac ctgctaatga atcttataaa
     9241 cctattaaaa taacagaaga aaatgatttt atagtgtggg gtatagttac ttacgtaatt
     9301 cataaaattt aaaaaaaaag taaaacttat aattttattc tgttttcata gagataaaac
     9361 ggtaaataaa aataaatctt tgattatttc ttcagtgata taacaaaatt ttataaaaat
     9421 tatataatag gtaataaaat aatagaggtt agagcatttt ataaataaat gagattgggg
     9481 ggatgataac attctttagt gtttaaatat acacaggact tgctctcaag tccctgtata
     9541 cataccacgc tcgc
//
LOCUS       DAOPRN010000068         9368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_6158_length_9368_cov_106.907334, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000068 DAOPRN010000000
VERSION     DAOPRN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6158_length_9368_cov_106.907334"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..1430)
                     /locus_tag="PKI65_07145"
     CDS             complement(282..1430)
                     /locus_tag="PKI65_07145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB78251.1"
                     /translation="MNQKLNIITLDIPYPPDYGGAIDEFYTIKYLHDQGVEIYLHAFE
                     YGSRKPNAVLEQYCKKVYYYPRNRSIKDFFNIKPFIVKTRDNKKLLRNLLDNKAPILF
                     EGIHTTFYLPHPALDDRIKIIRTHNIEQDYYRLLAKSESNKLKKIYFYTEALKLKNYE
                     KIIISKANAVAAITTGDQKIVKKWNKNTECISSFHPFNKVMSLEGRGSYALFHGNLNV
                     IENKIAAKFLSDKVFNDLNIPLIIAGKCSDEIKFKKKLEKNPNITLLTNLSDEKIKKL
                     IQNAQLNVLYTTQPTGLKLKLIHAIFLGRYIIANNLMVQNNELGPICEIANDEKEIKT
                     KVLDLFNKPFTNEEILKRENLLIEFGYMNDQNIQRLLQIIKMQSSTTE"
     gene            1556..2590
                     /locus_tag="PKI65_07150"
     CDS             1556..2590
                     /locus_tag="PKI65_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78252.1"
                     /translation="MCKILKYIFPLIIFVSLFSCSTDVDLIQEWQDIPVVYAILDVNK
                     QTQYIRINRAFLGVGDAYELAHSPDSINYDYLLDVTMKVYDKNNNFIKEYTCDTAHLW
                     YDSDIFYKGYMPIYRFKVSTPYQIDYYDTTWLNPDYKYELVIYNSKYDKFITSTCELI
                     GDININKPYPGQTWIAFNTSSKNSIEWTSAKKGRLYEALFRFHYKEVNNNNPSDTIYK
                     FIDWNIGSAKSNSLNGGENLSISYYNQGFFDNLNKITIDNNVVRIIGKVELIIIVKDD
                     ILATYISLYNSSSYSISQSRPVFTNIENGIGILACKRTFKRNYSLNSSTQIMLTTRED
                     LVPLNFVSPT"
     gene            2596..3015
                     /locus_tag="PKI65_07155"
     CDS             2596..3015
                     /locus_tag="PKI65_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78253.1"
                     /translation="MKIKTPLFLKILGIFTLIGSSLRIIASISVLISKELISFWLQFP
                     GFSFLEIYSNIFFNSLLIISSALSFLAVLFMWRQKIIGFYIYLMAQWGLILIRMIFYK
                     PIFITFLDELPNILLTIVFSISYAYYIPYFKSLTIED"
     gene            complement(3109..3182)
                     /locus_tag="PKI65_07160"
     tRNA            complement(3109..3182)
                     /locus_tag="PKI65_07160"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3146..3148),aa:Asn,seq:gtt)
     gene            complement(3245..4831)
                     /locus_tag="PKI65_07165"
     CDS             complement(3245..4831)
                     /locus_tag="PKI65_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78254.1"
                     /translation="MYKKIPIFVFITILFSINIFSQKNKTYSSGGTYPFSKDMMQIIH
                     FNSLSDNNILFFSKVGDNYKVELIDTKKLSINKSLDIPHKIKVGGYSYQIDYINSFII
                     NDNLYVFYKLYDRNKKGNALWYNVYDGKNLNMVQEYTELAFYQFDTRDKMLAGDFEVY
                     INPFKTIFFISQPIPRHPYEREKLKYTALDTDFNILWEKDVDFKYLDKDLAIQQISID
                     NVGNAFILLNNLLNKNIYQLISITDEGKKINNIMLSSIMDVKCLSPRLYPTENGTFII
                     SFKGDLENEVIDNLLFYKYDVLADSIQIFSSIPLTKTFLSSNFLDKGKKTKAEIDERK
                     KFIEELAFAIDTVIFIDEDIYLIAEKRFNYQDEYMLNDPQTGTQRVNVISNYNAQDIY
                     VFKINNKGKLIWSKKINKYQTTNNDNLSFLLGYSFGLVNDIFYFVFNDNINNNEGNDI
                     LEAPLSGNESCITLATIDKDGNIIRNVILSYNDINGAMIPSLSLVQASGDLFLSIAPL
                     RKSVSQFTKIKECYFLYLKP"
     gene            4963..6999
                     /locus_tag="PKI65_07170"
     CDS             4963..6999
                     /locus_tag="PKI65_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78255.1"
                     /translation="MKNQLIFILMISILLLASCSKQTSKYPYLDEKIVTNTIDTLLNK
                     HSHADSMRIRKGVEQVATLWYREDGSALDFKDFCINYFIPQSDELEQAFDRLCFYFES
                     LYGNNLQILLDFRKNIDEDTGPLNFLDEMFGSYDPFAHITDDFFKNKIAFVIRLNFPY
                     YTLAEKNKVGMNWTPRQWGYARLGDMFTSRIPADLQQKLTQTLAKAETYIATYNIFMG
                     NIVDDNGKIMFDTSDILISHWGLRDKLKTFYAKKDGLLYQKTIYSIMKRIIDQTIPIE
                     VINNDQYLWNPINNILYSNGKEIEGKQEENRRYQVWLNNFQAIKELDPFYPEYKNYIE
                     LSFDGVMELSFDEVENLFTTYLASPLLKDIGKLIEQRLGRPLEAFDIWYDGFKPRASI
                     DETKLNAITKAKFKSAEDFEKEMANMLIYLGFQKDTAEYIAKYIEVDPARGSGHAWGA
                     ESKNMKSHLRTRIAEDGINYLGFNIASHEFGHNVEQTISLHFVDNYFIHGVPNTAFTE
                     ACAFLFQARDLDLLGIKNQDPLQHYLNTLDVAWSLFEIIGVALVDMYTWQWIYEHPNA
                     TANELKETVIDNAKNIWNKYYAPVFGIKNEPILAIYSHMINDPLYLPNYPIGHIISFQ
                     LEEFLKDKTFGKEIIRIYSIGRLTPQVWMQKAIGQNISIEPLLNEIKLSIEKIK"
     gene            7055..7219
                     /locus_tag="PKI65_07175"
     CDS             7055..7219
                     /locus_tag="PKI65_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78256.1"
                     /translation="MNIIELKKQRKEKNKADFLIHINPKNIITLEAKLYSNIEVCEKQ
                     FFTYQNNLDK"
     gene            7222..7353
                     /locus_tag="PKI65_07180"
     CDS             7222..7353
                     /locus_tag="PKI65_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78257.1"
                     /translation="MYWREILWLISWPLMIWILHLIITSAINKYEDKTDNNKPKISD"
     gene            complement(7439..8287)
                     /gene="folP"
                     /locus_tag="PKI65_07185"
     CDS             complement(7439..8287)
                     /gene="folP"
                     /locus_tag="PKI65_07185"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOB78258.1"
                     /translation="MKEVTFNIKGRLIQLQFPAIMGIINLTPDSYYENSRMNSVDVVL
                     QKVEMMINDGVDIIDIGAESTRPQSTPLTAKEECKRLYKVLPILRKTFPDILISLDTY
                     HSETAKYGIDEGVDFINDISSGLMDPSIIQYVAESHIPYIAMHMLGTPATMQLDPHYE
                     NVVKEINYFFSERIHFLHEAGINDIIIDPGFGFGKSVDHNYQLLNHLYEFTMHQVPIL
                     VGLSRKSMIYKPLKLSPKDTLCATSAIHLQALLNGADILRVHDVKEANHIVQIYKLII
                     KNTGLN"
     gene            8330..8989
                     /gene="tmk"
                     /locus_tag="PKI65_07190"
     CDS             8330..8989
                     /gene="tmk"
                     /locus_tag="PKI65_07190"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOB78259.1"
                     /translation="MSKGNFIVIEGLDGCGKSTQIALLAKALQQREINTYAIHFPRRS
                     DEESPLWGSMIKKFLDGYYGDIDIVHPELVALLFAGDRYNAAKKINEQLNNNNWVLCD
                     RYVLSNIAYQGAKLPEEQWPSFANWILKTEYEYFKIPKPDLQIILSMPFSFIQKNLRS
                     RKLKLEDDIHEQSLSFLKNVDKMYKWIVENINNDIKYLNFNEVKDVSDTHKTIMSLLN
                     N"
BASE COUNT         3403 a         1234 c         1256 g         3475 t
ORIGIN      
        1 tttatacagg gacttgagag ctaagccaat attaaattta acataaaaaa attttctccc
       61 atttcttccg tttcaaaaaa ttagactttt tatccattaa ttttcttttc cctctcacat
      121 ctgctcctga tcttagtttc ttacatacca ccacttcttt ttttaattat attaagtttt
      181 cctagcaagt atagttaaaa tattaccaaa tatgaatttc tgcataaatg tatttttttt
      241 ggtgtctagg cattttcttt ttataccttc aaagatttta tttattcagt agtagatgat
      301 tgcattttaa ttatttgcaa aagtctttga atgttttgat cattcatata accaaattct
      361 attagtagat tttctctttt gagaatttct tcattagtaa atggtttgtt aaaaagatct
      421 aaaactttag tttttatttc tttctcatca tttgctattt cacaaatagg tcctagttca
      481 ttattctgta ccatcaaatt attagctatt atatatctac ctaaaaaaat tgcatgaatc
      541 aattttaatt taagtccagt tggctgtgta gtatataata catttagttg agcattttga
      601 ataagttttt ttattttttc atctgataaa tttgttaata gagtaatatt aggatttttt
      661 tctaattttt ttttaaattt tatctcatca gagcatttgc ctgctattat taaaggaata
      721 tttaaatcat taaacacttt atcagataaa aacttcgctg ctattttatt ttcgattaca
      781 ttgagattac catgaaatag tgcatagctt cctcgccctt ctaatgacat cactttattg
      841 aagggatgaa atgagctgat acattcagta ttcttattcc atttttttac aattttttga
      901 tctcctgttg ttatagcagc tacagcattg gccttactaa taataatttt ttcatagttt
      961 tttaacttta aagcttctgt ataaaaataa atttttttta atttattaga ttcacttttg
     1021 gcaagtaaac gatagtagtc ttgttctata ttatgtgtcc taattatctt tatcctatca
     1081 tctaatgcgg gatgcggtag ataaaatgta gtatgaatac cctcaaataa aatgggagcc
     1141 ttattatcta ataaatttct taataatttt ttattatcac gtgttttaac aataaaaggt
     1201 ttaatattaa aaaaatcttt aatgctacga ttacgtggat aataataaac ttttttacaa
     1261 tactgttcta aaactgcatt aggttttcta ctgccatatt cgaaagcatg tagatatatt
     1321 tctacccctt gatcatgcaa atattttatt gtataaaatt catcaatagc accaccataa
     1381 tcaggcggat agggaatatc taaagttata atattaagtt tttgattcat aaatcttatt
     1441 tatatataac atatttatct acaacaaaag tatattaaaa ttttgatttt atgaattgat
     1501 ttattaacgt aaataatttt tattaaaata atttttcata tttttgaaaa aaaatatgtg
     1561 taaaatttta aaatacattt tcccattaat aatttttgta agtttatttt cttgttccac
     1621 agatgtcgat ctcatacaag agtggcagga tatacctgtg gtatatgcta ttttagatgt
     1681 aaataaacaa acgcaatata ttagaattaa tagagctttt ctaggagtgg gtgatgctta
     1741 cgaattagca cattcacctg atagtatcaa ttatgattat ttgttagatg taactatgaa
     1801 agtttacgat aaaaacaata atttcataaa agaatataca tgtgatactg ctcatttatg
     1861 gtatgattct gatatttttt ataaaggata tatgcctatt tatagattta aagtatctac
     1921 accttatcaa atagattact atgatactac ctggctaaac cctgattata aatatgaact
     1981 agtgatatat aattctaaat atgataaatt tataacttca acatgcgagt taataggaga
     2041 tattaatatt aataaaccat atccgggaca aacatggatc gcatttaata catctagcaa
     2101 gaatagtata gaatggacta gtgctaaaaa aggtagacta tatgaagctt tatttcgttt
     2161 tcattataaa gaagtaaaca ataataatcc aagtgataca atttacaaat ttatagattg
     2221 gaatattgga tcagctaaaa gcaattcatt aaatggtggt gaaaatttat ctattagcta
     2281 ttataatcaa ggattttttg ataatttgaa taaaataact attgataata atgtggtccg
     2341 tattattggc aaagtagaac taattattat tgtaaaagat gatattttag ctacatatat
     2401 atcactgtat aattctagtt catattctat ttcacaatct agacctgtat ttactaacat
     2461 agaaaatgga attggcattt tagcttgtaa acgtactttt aaaagaaatt attctttgaa
     2521 ctctagtact cagataatgt tgacaacaag ggaagattta gtaccactta attttgtatc
     2581 acctacataa taaaaatgaa aataaagacg cctctttttt taaaaatttt aggtatattt
     2641 actttaattg gatcttcatt aaggatcatt gcttctatat cagttttgat ttcaaaagaa
     2701 ttaataagtt tttggttaca gtttcctggt tttagttttt tagaaattta ttctaatatt
     2761 ttttttaata gtttactaat tatttcctca gctttatcat ttttagctgt tttattcatg
     2821 tggagacaaa aaataattgg cttttatatt tatttaatgg cccaatgggg attaatttta
     2881 attagaatga ttttttataa acctatattt atcacatttt tagatgaatt gcctaatatt
     2941 ttactcacta ttgtattttc gatttcttat gcttactaca taccatattt taaaagtcta
     3001 acaattgaag attaatatct aggaaatatc taattatatt aatttgattt aaaattcggt
     3061 tttttctttg tatactaatg cttattaaat taatataaaa ctcttgagct ccctaggtag
     3121 gacttgaacc tacgaccctc tgattaacag tcagatgctc taaccaactg agctactagg
     3181 gaatattttt attttgcaaa attataaaat attttttata tgaaaaaata tttttataat
     3241 ttttttatgg ttttaaataa agaaaataac attctttgat tttagtaaat tgtgaaacag
     3301 atttacgtag tggagctata gataaaaata aatctcctga agcttgaact aatgataaac
     3361 taggtatcat agctccattt atatcattat aactaagtat aacattgcga ataatattac
     3421 cgtctttatc gatagtagct aaagtaatgc atgattcgtt acctgatagt ggcgcttcta
     3481 atatatcatt accttcatta ttattaatat tatcattaaa aacaaaataa aaaatatcat
     3541 ttactaaccc aaaagaatat cctagcaaaa aagataaatt atcattatta gttgtttgat
     3601 atttatttat ttttttactc catattagtt tccctttatt gtttatttta aatacatata
     3661 tatcttgtgc attataatta ctaatcacat ttactcgttg agtaccagtt tgtggatcat
     3721 ttagcatata ttcatcttga taattaaaac gtttctctgc tattaaataa atatcttcat
     3781 caataaaaat gacagtatct attgcaaaag ccaattcttc aataaatttt tttctttcat
     3841 ctatctcggc tttagttttt ttgcctttat ctaaaaaatt actagaaaga aaagtttttg
     3901 taagaggaat actagaaaat atctgaatac tatcagctaa aacatcatat ttataaaaaa
     3961 gtaaattatc tatgacttca ttttctaaat cacctttaaa gctaataata aaggtcccat
     4021 tttcagtagg atataagcgc ggagataagc atttaacatc cataatagat gagagcataa
     4081 tgttatttat ttttttacct tcatcagtta tagatattag ctgatatata tttttgttta
     4141 ataaattatt taataaaata aatgcattcc ccacattatc gattgatatt tgctgtatcg
     4201 ctagatcttt atctaaatat ttaaaatcta cgtctttttc ccataatata ttaaaatctg
     4261 tatctaatgc tgtatatttc aatttttctc tttcatacgg atgtcgaggt ataggttgtg
     4321 atataaaaaa aatagttttg aatggattta tatatacttc gaaatcacca gctaacatct
     4381 tatctctggt atcaaattga taaaaggcaa gctcagtgta ttcttgtacc atatttagat
     4441 ttttaccatc gtatacattg taccatagag cattaccttt tttatttcta tcatacaatt
     4501 tgtaaaaaac ataaagatta tcattaatga taaatgaatt aatgtaatca atttgatatg
     4561 aatatccacc tacttttatt ttgtgtggta tatcgagcga tttattaatc gacaattttt
     4621 tagtatctat taactctact ttataattat cacctacttt agaaaaaaat aaaatattat
     4681 tatctgataa actattaaaa tgtataatct gcatcatatc tttcgaaaac gggtaagtgc
     4741 ctccactaga ataagtttta tttttttgcg aaaatatatt aattgaaaat aatatagtta
     4801 taaaaacaaa aattggaatt tttttataca taataaatta aattttactg caaaattaaa
     4861 gaatttttaa taataaaaat attctctttt ttttactatt tcattgatat tgaaaatata
     4921 tttataattt ttttattata aatttgcata aaatacaata ttatgaaaaa tcaattaatt
     4981 tttatcctta tgatttcaat tttattatta gctagttgca gtaagcaaac ctcaaaatat
     5041 ccttatttag atgaaaaaat agtaacaaat accattgata cattgctaaa taaacatagc
     5101 catgctgata gtatgcgcat cagaaaggga gtagagcaag tagctacact gtggtaccga
     5161 gaagatggct cagcattgga ttttaaagat ttttgtatca attattttat acctcaaagc
     5221 gatgaactag agcaggcttt tgatagatta tgcttttatt ttgaatcatt gtacggtaat
     5281 aacttgcaaa tattattaga ttttagaaaa aatattgatg aagatacagg tcctttaaat
     5341 ttcctagatg agatgtttgg tagttatgat ccttttgctc atataacaga tgattttttc
     5401 aaaaataaaa tagcttttgt tatcagatta aatttccctt attatacatt agcagaaaaa
     5461 aataaagtgg gaatgaattg gacaccacgt caatggggtt atgctagact gggtgatatg
     5521 tttacatctc gtatacctgc tgatctacag caaaagctta ctcaaacttt agcaaaagcc
     5581 gaaacttata ttgctactta caatatattc atgggcaaca tagtagatga taatggtaaa
     5641 ataatgtttg atacctctga tatattaatc tctcactggg gattacgtga taaattaaaa
     5701 actttttatg ctaaaaaaga tggattatta tatcaaaaaa ctatttactc tattatgaaa
     5761 cgtataatag atcaaactat tcctatagaa gtgatcaata acgatcaata tttatggaat
     5821 ccgataaata atatattata ttctaatgga aaagaaatag aaggtaaaca agaagaaaat
     5881 cgaagatatc aagtatggct aaataatttt caagctatca aagagttaga tcctttttat
     5941 ccagaatata aaaattacat tgaattatca tttgacggcg taatggagct atcatttgac
     6001 gaagtagaaa atcttttcac tacttattta gcatctccat tattaaaaga tattggtaaa
     6061 ttaatagaac agcgcttagg tagaccatta gaagcttttg atatatggta tgatggattt
     6121 aaaccgagag cttcaataga tgagaccaaa ttaaatgcta tcactaaagc caaattcaaa
     6181 tctgcagaag attttgaaaa agaaatggca aatatgctaa tatatttagg ttttcaaaaa
     6241 gatacagcag aatatattgc taaatacata gaagtagatc cggctagagg ttctgggcat
     6301 gcatggggtg ctgaatcaaa aaatatgaaa agtcatctaa gaactagaat agctgaagat
     6361 ggtataaact atttaggttt taatattgct tctcatgaat ttggtcataa tgtagagcaa
     6421 accatttcat tacattttgt agataattat ttcattcacg gagttcctaa tactgcattt
     6481 acagaggcgt gtgccttttt attccaagct agagatttag atctattagg tataaaaaat
     6541 caagaccctt tacaacatta ccttaatact ctcgacgtag catggtctct atttgaaatc
     6601 ataggtgtgg ccctagtaga tatgtatacc tggcaatgga tatatgagca tcctaatgct
     6661 acagctaatg aactaaaaga aacagtcatt gataatgcta aaaatatatg gaataaatat
     6721 tatgccccag tatttggaat aaaaaatgaa cctatcctgg ccatctatag tcatatgata
     6781 aatgatcctc tatatttgcc taattaccct attggccata ttatttcttt tcaattagaa
     6841 gaattcctaa aagataaaac ttttggtaaa gaaattattc gtatctatag tattggaaga
     6901 ttgactccac aagtatggat gcaaaaagct ataggccaga atatatcgat agaaccttta
     6961 ttaaatgaaa taaaacttag catagaaaaa ataaaatgat attgtaatga tattataaca
     7021 aataagttat attgattttg ataaaaatac taaaatgaat attatagaat taaaaaaaca
     7081 acggaaagaa aaaaataaag cagatttcct aattcacatt aatcctaaaa atataatcac
     7141 cttagaagct aagctataca gcaacataga agtatgcgaa aaacaatttt tcacctatca
     7201 aaataattta gataaataat tatgtattgg agagaaatat tatggctcat atcctggcca
     7261 ctgatgatat ggatattgca tctaatcatc actagcgcta ttaataaata tgaagataag
     7321 acagataata ataaacccaa aataagtgat taaaatttta atcgcttact ttaaagaata
     7381 tgaaatgttt tttaagcaat aaaaaaaaat aattctcaaa ctcaattttt attttttttt
     7441 aattgagacc tgtatttttt ataatcaatt tataaatctg aacgatatga tttgcttctt
     7501 tcacgtcatg tactcttaat atatctgccc catttaataa tgcttgcaaa tgaatagcag
     7561 aagtagcaca caaggtgtct ttaggactga gttttaatgg tttataaatc atactttttc
     7621 tagataaacc taccaaaatt ggaacttgat gcattgtaaa ttcgtataga tgatttagta
     7681 attggtaatt atgatctaca gatttgccaa agccaaagcc aggatctata attatatcat
     7741 ttatacctgc ttcatgtaaa aaatgtattc tctcagaaaa aaaataatta atttctttaa
     7801 ctacattttc ataatgaggg tctaactgca tggtggctgg tgttcctaac atatgcatag
     7861 ctatatatgg gatatggctt tcggctacat attgtatgat actaggatcc attagtccgc
     7921 ttgagatatc attaatgaag tctactcctt cgtctatacc atatttagca gtctcactat
     7981 gataagtatc aagagatatc aatatatcgg gaaaagtttt tcttaaaatt ggtaaaactt
     8041 tataaagtct tttgcactcc tctttagcag ttagtggtgt tgattgtgga cgtgtagatt
     8101 cagctcctat gtctataata tctacaccat catttatcat catttccact ttttggagta
     8161 ctacgtctac agaattcatt ctgctatttt cataatatga gtctggcgtt agatttatta
     8221 tacccataat agctgggaat tgtaattgta ttaatctgcc tttgatatta aatgtaactt
     8281 ctttcatttt tattatttaa ttttacaaaa ttagaaaaat aattctgaaa tgagcaaagg
     8341 taatttcata gtaatagaag gcttagatgg ttgtggtaaa tctacacaaa tagcactctt
     8401 ggcaaaagct ttgcagcagc gcgaaataaa tacttatgca attcatttcc ctagacgtag
     8461 tgatgaggaa tctccactat ggggaagcat gattaaaaaa tttttagatg gatactatgg
     8521 agacatagat atagtacatc cagagcttgt ggctttatta ttcgctggtg atagatataa
     8581 tgctgctaaa aaaataaatg aacaattgaa taataataat tgggtgctgt gtgatagata
     8641 tgtactgtct aatattgctt atcagggagc taaattaccc gaagagcaat ggccttcatt
     8701 tgcaaattgg atcttaaaaa ctgaatatga atattttaaa attccaaagc cagatttaca
     8761 aattattctt tctatgcctt ttagttttat tcagaaaaat ttaagatcta gaaagctaaa
     8821 attagaagat gatattcacg aacaatcttt atctttttta aaaaatgtag ataaaatgta
     8881 taaatggatt gttgaaaata taaataatga tattaaatat ttaaatttca atgaagtaaa
     8941 agatgtaagt gatactcata aaactataat gagcttgcta aataattaat tggttcaata
     9001 atgttttata tggactaaag attaaaaaaa gagtaattat acaagaaatt cgttaatatt
     9061 ggttttttta ttgaacagtt aaatatcaac tactaagttc tctgagaata cacaaagaac
     9121 gcagaaaagg atggaattaa aagggctaca aatagctctg tgtaataaaa aaaataacct
     9181 aaataattaa aaaatagtta tttttttgta acaatctata atttgattac ccaaattata
     9241 acctaagata tgtgggagtg gagaattgga aacttgaaac ggatcagtcg gaaaattaat
     9301 atatgttaaa atcaacatat gttatattta ctaactccct ataccgatac cacgcccatc
     9361 tgtccgag
//
LOCUS       DAOPRN010000069         8727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_6711_length_8727_cov_159.043589, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000069 DAOPRN010000000
VERSION     DAOPRN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6711_length_8727_cov_159.043589"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="PKI65_07195"
     CDS             complement(<1..1105)
                     /locus_tag="PKI65_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78260.1"
                     /translation="MLKIYKLFIFISLITISKLTYSQINVDILTQVDSSFISKDTISI
                     CAENVIGLYANVSGGTSPYTYDWYGADSLLMGTSSPFTTFIASNPGTYMIGCLVTDDY
                     GNVCKDSVIIIVKALPDLILSPLNDSICIGDSITLTASGASTILWFDDNDNLIGWGSQ
                     IIISPTSNTNYFVVGVQSGCSKTQSINIKVFQPPIVNAGNDTIICSLDSISLHASASN
                     YLNIQWTSNGTGNYTNTNDLNTIYIPSTQDWANGSVILTLTASGYADCPDAHDQLTIN
                     FIDVPDLISSTDTTICEGESVTISANGANSFLWSEGSNTSSIIVSPSLNTTYYVTGSI
                     DICQRIDSFIVYVNPAPIFNLGNDTTICYNDSVT"
     gene            1217..1639
                     /locus_tag="PKI65_07200"
     CDS             1217..1639
                     /locus_tag="PKI65_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOB78261.1"
                     /translation="MKKITHEGVITKITNNEIEVTVKVTGACASCQLNKMCISTESAE
                     KIIIVPKKDNDNYRLGEHVEISSTTRQGYVAVCLVYVLPVILLVTAYFISFSYFDNEL
                     LSASVSIGIIILYYILIYFLNPLIKNKISFKLKHINYN"
     gene            1799..2413
                     /gene="rsmG"
                     /locus_tag="PKI65_07205"
     CDS             1799..2413
                     /gene="rsmG"
                     /locus_tag="PKI65_07205"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOB78262.1"
                     /translation="MSDLIKEYFPELSKKQLILLEDYKKLLLDWNSKVNLISRKDQNH
                     IDEHHLLHSLSISKFYQFLPNSKVIDIGTGGGLPGIPLAILFPETEFILIDSRKNKIN
                     ALKDIIKRLNLPNVIAINERVESIIFQYHLAIGRAVSDLKVIINWINKNVKPLPGEPF
                     LIYLKGIDAFDELKNIPYKYTIRPINEFVKLTYYQDKYIIMLYK"
     gene            complement(2515..3462)
                     /locus_tag="PKI65_07210"
     CDS             complement(2515..3462)
                     /locus_tag="PKI65_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOB78263.1"
                     /translation="MNKVIALDLGGSHLTIALANQNNIITKNKYNINQFNVFNDVIDE
                     IMSFYDKHNLNNEIIGIGIGAPNANYHTGCIHNAVNLPWKEVINVKKLLSQKFPHLIN
                     VIDNDANCAALGEKYYGSAKNIKDFLVITLGTGLGSGIYCNDKIVRGATGLAGELGHV
                     ILINNGRLCTCGRNGCVESYISTRGIFQTAIELATHYDNSSLFEYPQDKWTPKLIANL
                     ASANDMLSQSIFDKTAEYLALSLANYITLLEPSGIFLTGGISQAGDILLKPLCKYINQ
                     YILPFHANSFKIDKSSLPEQNAALLGVAALVWQEWQEKL"
     gene            3586..4887
                     /locus_tag="PKI65_07215"
     CDS             3586..4887
                     /locus_tag="PKI65_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOB78264.1"
                     /translation="MKSPSIKDYYNSIIKKAKSQKNFLSLLSGNVISQIIPFLLAPII
                     ARIFTPVDFAVQANFIALIMLISSVAAGRYEVAIVLPKNWRKARAIATLSFILIIIVS
                     IFSNVLLLLKDVVARLYNDNILPQYLWLVSISVLIVASYNTFHNIGLRYGLFKNLSYS
                     KIAQSTFGQLMIAAWGYWQWGGNGLIFGWLLGQLLSMIYLFVVIKQKTNFRPLWNFKL
                     IKKVAKEYKKFPLVNSLHILADTFSQQFFLFWLITFFFGSETLGFFAVVVKYLRIPIQ
                     LIGNALSQVFYKEAVQTYQNRKSLRALYLKNFKTTLIFAIPFLLIIGLGGKVLFTFYL
                     GNQWTQAGIYAQYYVLSMFFLFIVSPVNSLPLVVDKQELHFALSMINYVLSLGTIIVM
                     ALLGYSFDTILIVYTLVQSIIYAFIGIWYYHLACKADKKII"
     gene            5066..6067
                     /locus_tag="PKI65_07220"
     CDS             5066..6067
                     /locus_tag="PKI65_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78265.1"
                     /translation="MNILISPLNWGLGHATRLVPIIRKLIENNNKVIIAGDGQSIVWL
                     KNYFSNIEIVHFPSFNFKYSKKGIKLSDYFILANQIVCQTLYENQKIKNILKLYSIDL
                     IISDNRYGFYSNTIPSFLITHQLDIILPNKLMFAKTLINMYLKKYFQKFDKIFIPDFE
                     DFNKSLAGALSHHKNQINVPIKYIGPLSRFSNKKMIYYDYYYDILILVSAPTKYMYEI
                     VNLLDFYADLYPYISFAMITPYNFYSNRDNLTFIVSPCDDEWLATVLGSKKIISIAGY
                     STIMDLYTIKKSAILIPVQGQTEQMYLANFLDGKHNFKSVDSLKSAIELAIMQFIIK"
     gene            complement(6160..7518)
                     /gene="radA"
                     /locus_tag="PKI65_07225"
     CDS             complement(6160..7518)
                     /gene="radA"
                     /locus_tag="PKI65_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOB78266.1"
                     /translation="MAKVTKIYICSNCGTRYSKWLGKCSNCGEWNTIEEQIINKTDVK
                     SNNLLTISPKLIQEINYDNFERISLPSDELNRILGGGLTRGSIILLAGEPGIGKTTLL
                     MQELMKIKDFTVLYVSGEESLGQLKYRSERLGRPNSQFYLFHELSLPIIIQAIEEVVP
                     DFVVIDSIQSISDPNLSGFTGSLQQIRECTSRLQEIAKVKNIPIFIIGHITKEGYIAG
                     PKALEHIVDTVMHFEGDKNYGYRVLRVIKNRFGPAPELAIFEMTQDGLKEINNISEYL
                     LETYKPDTPGSSIAVLMEGNRPILVEVQALVSYSPYGTPQRSSIGYDPHRLGMILAVL
                     EKRLDLRVNNRDIFVNVTGGLRIYDPAIDSAVVAAILSSFNDKTLPHLLALSAEIGLN
                     GELRSVSQLQKRIQEASKLGFKQLITANIKEKSFKNESFNSSINLKFINNISELAQVL
                     FF"
     gene            complement(7753..>8727)
                     /locus_tag="PKI65_07230"
     CDS             complement(7753..>8727)
                     /locus_tag="PKI65_07230"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78267.1"
                     /translation="SKYLAMSKEENKEFLDNLFSLMKKLEADVVDYQGLKILSAPPLC
                     PDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYIRCMVAGKS
                     LRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDCSEKGRIYKNV
                     EELKRARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMPKDGVMCSS
                     NKKAFKKVRRNVIKHKEIVTNHRQKRGIYSLSTVKKKASEFTNWIYFKFRGVATKYLQ
                     NYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVKLTYVD"
BASE COUNT         2974 a         1340 c         1197 g         3216 t
ORIGIN      
        1 ttgtaacact atcattataa caaattgttg tgtcattacc taaattaaat attggagctg
       61 gattaacata tactataaag ctatctattc tctgacaaat atcaatagat cctgtaacat
      121 aatatgtggt attaagggaa ggagatacaa taatagaaga tgtattactt ccttcgctcc
      181 acaaaaatga attagcgcca ttagctgaaa tggtaacact ctcgccttcg catatagtag
      241 tatctgtaga agaaataagg tcaggaacat caatgaaatt aatagttaat tgatcgtgag
      301 cgtctggaca atccgcataa ccactagctg taagtgttaa tatgacagaa ccattagccc
      361 aatcttgagt cgaaggtata taaatagtat ttaaatcatt agtatttgta taatttccag
      421 ttccattcga tgtccattga atatttaaat agtttgaagc tgaagcatgt agggaaatac
      481 tatccaaact acatataatt gtatcatttc cggcatttac tataggtggt tgaaaaactt
      541 taatatttat agattgagtt ttgctacagc cactttgcac tcctacaaca aaataattag
      601 tattagaggt aggagatatg attatctgag atccccaccc aataaggttg tcattatcat
      661 caaaccacaa tatagtgctt gctccactag ctgttaaagt aatagaatcc ccaatgcata
      721 tactatcatt tagaggtgat agtattagat ctggcaaagc ctttactatt attattacac
      781 tatctttgca aacattacca taatcatcag ttactagaca ccctatcatg tatgttccag
      841 gattcgaggc gatgaaagtt gtaaatggtg aagaggtccc cattaataaa ctgtctgccc
      901 cataccaatc atatgtataa ggagaagtgc cgccagatac attagcatac aatccgatta
      961 cattttctgc acaaatacta atagtatctt tacttataaa agaactatct acttgagtta
     1021 gtatgtctac atttatttga ctataagtta attttgatat agtaattaat gaaataaata
     1081 taaataattt atatattttt aacataatat tgtatgtttg atttggtaaa attatatatt
     1141 tattttcaat tttttatata tttttcaatg aaataattta taatttttta tttttgtata
     1201 ataattaata taataaatga aaaaaataac acatgaaggg gttattacaa aaattacgaa
     1261 taatgaaatc gaagttactg taaaggttac tggtgcatgt gcatcgtgtc aattaaataa
     1321 aatgtgtata tctactgaaa gtgctgaaaa aataataata gtccctaaaa aagataatga
     1381 taactatcga ttaggcgaac atgtagaaat tagctccaca actcgccaag gatatgtagc
     1441 agtatgctta gtatatgttt tacctgtaat tttattagta actgcctatt ttatatcttt
     1501 ttcttatttt gataatgaac tattatcagc aagtgtctct ataggaatca ttattttata
     1561 ttatatttta atttattttt tgaatccatt aattaaaaat aaaatttctt ttaaattgaa
     1621 acacattaat tacaattaat ctaatttaaa aatcaaataa aactatcaaa gtatagcaca
     1681 gaaaatttta aatgtctaat ttttttttaa ttttgtttaa tcacaatatt ttataaaaat
     1741 aaatggaccg cttggcaaat aaattgataa aataaataca taaattttga aataataaat
     1801 gtcagattta ataaaagaat atttcccaga attatctaaa aagcaattaa ttttgttaga
     1861 agattacaaa aaacttttat tagattggaa ctcaaaagta aatctaatat ctcgcaaaga
     1921 tcaaaaccat attgatgaac atcatttatt acattctcta tctataagta aattttatca
     1981 atttttacct aattccaaag taatagatat aggaactggt ggtggtttac caggtatccc
     2041 tttggctatt ttattccctg aaaccgagtt tatattgatt gattcacgca aaaacaagat
     2101 aaatgcttta aaagatatta ttaaaagatt aaatctaccc aatgtaatag ctataaatga
     2161 aagagtagaa tctattattt ttcaatatca tctagctata ggaagagctg ttagtgattt
     2221 aaaagtaata attaattgga ttaataaaaa tgtaaaacct ctaccaggtg agccattttt
     2281 aatatattta aaaggaatag atgcttttga tgaattaaaa aatattcctt ataaatatac
     2341 tattcgcccc ataaatgagt ttgtcaaatt gacttattat caagacaaat acatcattat
     2401 gttatacaaa taaaataata atgcaaccaa tgaatattaa taattttaat tcacaaccca
     2461 cacttgatta acccccacta cttattaact cttagtacct tcacagtttt gatcttacaa
     2521 tttctcctgc cactcttgcc acactaaagc tgctacgcct agtagtgctg cattttgttc
     2581 aggcaaactc gatttatcaa tcttaaatga attggcatga aatggcaaaa tatattgatt
     2641 aatgtatttg cataatggtt tgagcaagat atcgcccgct tgactaatac ctccggtcaa
     2701 aaatatacct gaaggttcga gcaaagtaat ataattagca aggctcagag caagatattc
     2761 agccgtttta tcaaatattg attgtgaaag catatcatta gcggatgcga gattagcaat
     2821 taacttagga gtccatttat cttgcggata ttcgaataaa gatgaattat cgtagtgtgt
     2881 agctaattca atagcagttt gaaaaatacc acgcgtactt atatagcttt ctacacaacc
     2941 atttctacca caagtgcata gccttccatt atttatcaaa atcacatgtc caagctcacc
     3001 agctaaacct gtagctccac gtactatttt atcattacaa taaataccac tacccaatcc
     3061 agtgccaaga gttataacta aaaaatcttt tatattttta gcagatccat aatatttttc
     3121 acctaaagca gcacaattag catcattatc gattacgttt attagatgag ggaatttctg
     3181 tgataaaagt tttttcacat ttatcacttc tttccatggt aaatttactg cattatgtat
     3241 gcagccagta tgataatttg catttggtgc tcctataccg attcctataa tttcattatt
     3301 taaattatgt ttgtcgtaga acgacattat ttcatctatc acatcattga atacattaaa
     3361 ttgatttatg ttatatttat ttttagtaat aatattattt tggtttgcca atgcaatggt
     3421 taaatgacta ccacctaaat ctagcgcaat tactttattc attataaata attttttgaa
     3481 ttgttaaaat taaaaagaat attttaaatt tatttaaact taaccatttt attatttgtt
     3541 taattaattt tgcaatgtaa caaattttaa tatttaagag ctttgatgaa atctccatct
     3601 attaaagact attacaatag cataattaaa aaggcaaaga gtcaaaaaaa ttttttatca
     3661 ttattaagtg gtaatgtaat atctcaaata ataccatttt tgctagctcc tatcatagct
     3721 aggattttta caccagtaga ttttgctgta caagctaatt ttatagcatt gataatgcta
     3781 ataagttcag tagctgcggg cagatatgaa gtagcaattg ttttaccaaa aaattggcga
     3841 aaagcgcgag ctatcgctac cttatcattt atattgataa ttatcgtatc tattttttcc
     3901 aatgttttat tattattaaa agatgtagta gctcgcttat ataacgataa cattttacca
     3961 caatatttat ggcttgttag tataagtgta ttgatagtcg cctcttacaa cacttttcat
     4021 aatattggtt tacgctatgg tcttttcaaa aatctttctt atagcaaaat agctcaatct
     4081 acctttggac aattgatgat agctgcatgg ggctattggc aatggggcgg taatggattg
     4141 atttttggat ggcttttagg tcagctttta tcaatgatat atttatttgt agtgattaag
     4201 caaaaaacta attttaggcc tttgtggaat tttaaactaa taaaaaaagt agcaaaagaa
     4261 tataaaaaat tcccattagt caatagttta catatattag cagatacttt ttctcaacaa
     4321 ttttttttgt tttggcttat cacgttcttt tttggctctg aaaccttagg tttttttgct
     4381 gtagttgtca aatatttacg tatacccatt cagttaatag gaaatgcact atctcaggtt
     4441 ttttataaag aagcggttca aacgtatcaa aatagaaaaa gtctgagagc cttatatttg
     4501 aaaaatttca aaactacact aatatttgcc attccttttc ttttaataat cggattgggt
     4561 ggcaaggtgt tatttacttt ttatctggga aatcaatgga cacaggcagg tatatatgct
     4621 caatattacg ttttatcaat gttttttctt tttatagtct caccagtcaa ttctttacca
     4681 ttagttgttg ataaacaaga gttacatttt gcattgtcta tgatcaatta tgtacttagt
     4741 cttggtacta taatagtaat ggcattactt ggttattcct ttgatactat tttgatagta
     4801 tatacactag tacaatctat tatttatgct ttcattggta tctggtatta tcatttagca
     4861 tgtaaagctg acaaaaaaat aatataaaaa atagttgaaa ttattataat ctaagtagtt
     4921 gaatataaca cagaagatta gctaaaacat caataattta tactatgaaa tactaaacaa
     4981 aattttttgc ttaatatgca tttatgtaat aaaatttcga aatttatatt tttataaata
     5041 attatttgta aaatttgaaa caaaaatgaa catattaata agtccattaa actgggggtt
     5101 gggccatgct actagattag tacctattat tagaaaatta atagaaaaca ataacaaagt
     5161 aataatagca ggtgatgggc aaagcatcgt atggctcaaa aattatttct ccaatataga
     5221 gattgtacat ttcccttctt ttaactttaa atattctaaa aaaggtatta aattgagtga
     5281 ttactttatt ttggcaaatc aaatagtttg tcaaacttta tatgaaaatc aaaaaatcaa
     5341 aaatattttg aagctatata gtatagacct tataattagt gacaatagat atggctttta
     5401 tagcaatacc ataccttcat ttttgattac tcatcaatta gatataatat tacccaataa
     5461 attaatgttt gctaaaacat tgattaatat gtatttaaaa aaatattttc aaaaatttga
     5521 taaaattttt attcctgatt ttgaagattt taataaaagt cttgcaggtg cattatcaca
     5581 tcataaaaat caaattaatg tacctataaa atatatagga ccattgtcta gattttcaaa
     5641 taaaaagatg atatattatg attattatta tgatatttta attttagtct cagcgcctac
     5701 gaaatacatg tatgaaatcg taaatttact tgatttttat gctgatttat atccttacat
     5761 tagttttgct atgatcacac cttataattt ttatagtaat agagataatt tgacttttat
     5821 tgtttctcct tgcgatgatg agtggttagc aacagtatta ggttctaaaa aaattattag
     5881 cattgcaggt tattcaacta taatggatct ttatactata aaaaaatccg ctattttgat
     5941 accagtgcaa ggtcaaacag aacaaatgta tttagctaat ttcttagatg gaaaacacaa
     6001 ttttaaatca gttgattcat taaaaagtgc aattgaatta gcaataatgc aatttataat
     6061 aaaatgataa tcttgatttt caaacaacaa taattttgat ataatttttt atatttataa
     6121 atattacata actgattatt ttaatttatt gatttatcat taaaaaaaca atacttgtgc
     6181 tagttcagaa atattattta taaatttcaa attaatcgat gaattaaaag attcattttt
     6241 aaaagatttt tctttgatat ttgcagtaat caattgttta aaaccaagtt tagaagcctc
     6301 ttgtattctt ttttgcaatt gagatactga tcgtagttct ccattcaaac ctatttcagc
     6361 agataaagct aataaatgtg gtaaagtttt atcattgaac gaggataata tggcagcgac
     6421 tacagcacta tcaatagctg gatcatatat ccgcaatccg ccagttacat ttacgaaaat
     6481 gtccctatta tttactcgca aatctaatct tttttctaat actgctagta tcattcccaa
     6541 tctatgagga tcgtatccta tagaagatcg ctgaggagtt ccatatggtg aatagcttac
     6601 gagtgcttgt acctctacga gtattggtct attaccctcc attaataccg caatgctact
     6661 accaggtgta tctggtttgt aagtttcaag taaatattca ctaatgttat ttatctcttt
     6721 taatccatct tgtgtcattt caaatattgc taattctgga gcaggaccaa agcgattttt
     6781 tataactcgc aaaacgcggt atccataatt tttatcacct tcgaagtgca ttaccgtatc
     6841 tactatatgt tctaaggctt taggcccagc tatgtatcct tcttttgtga tatgtcctat
     6901 aataaaaata ggaatatttt ttaccttcgc tatctcttgt aatcttgacg tgcattctct
     6961 gatttgttgc agactaccag taaatccgct taaatttgga tcagaaatag actgtatgct
     7021 atcaatcact acaaaatcgg gtactacttc ttctatagct tgtataatta ttggcaatga
     7081 tagttcgtga aataaataaa attgcgaatt tggccgtcct aatctttcgc tacgatattt
     7141 tagttgtcct aagctctctt ctcctgatac atataatact gtaaaatctt ttattttcat
     7201 taattcttgc attagtagag ttgtttttcc tatgccaggt tcaccagcta ataaaattat
     7261 tgatcctcta gtaagcccac ctcccaatat tctatttagc tcatcactag gcaaagaaat
     7321 cctttcaaaa ttatcatagt ttatttcttg tattaatttt ggagatattg ttaatagatt
     7381 atttgatttt acatcagttt tattaatgat ttgttcttct atggtattcc actctccaca
     7441 gttagagcat ttgcctaacc atttactata cctagtacca cagttagaac atatatatat
     7501 tttcgtaact tttgccatag atctttttaa tacaaattaa tacaaatttt tttatttgaa
     7561 tattttttat ttagtacaaa aaaattaaaa ttattagtat tatctcggtt ctataaggtt
     7621 ttacatgagt aaagattaaa aaaagagtaa ttctacaaaa aaatttgatt acccaaatta
     7681 taacctaaaa tatgttggat gtggagaatt ggaaacttga aaactaatca atttggaaaa
     7741 ctaatatatg tttcaatcaa catatgttag tttcactaat tccctatacc gataccatgc
     7801 ccacctgtcc gaggatgcga ggttgagcat tcgcccagta ttgcttacag tcttgcctgt
     7861 gaggtattta cccatcacta cataccacat aatgtagttt tgcaggtatt tggtagctac
     7921 acccctaaat ttgaaatata tccaattcgt gaattcacta gcctttttct tgacagtact
     7981 taggctatat atacctcgct tttgtctatg gttagttact atttctttgt gcttgattac
     8041 attccttcta acctttttga atgctttttt attactgcta cacatgactc catcctttgg
     8101 cattttagcc ttcagaaatt gctctatttg ttctgaactt agctttttgc catcaaattt
     8161 tttgaataat aagttgccag ctctgtctgt agcagccaat acggctactt tctttggctt
     8221 ttgcttcctg gctcgcttca gctcttcgac atttttgtat atcctgcctt tttcggaaca
     8281 atccatcaat agttcgtcca tctctactac tccgaaaaaa tttatattat cttcgaaact
     8341 ttgtagagct gctaatatct tgtgtcgcca agcaaagctt gttggtaggc tgatgcccac
     8401 ctctttagca caagctctga gacttttgcc tgctaccata catctgatgt agtcgagcat
     8461 tttttctttc ttttgcaacc tgtaaacaaa agtacctgtg gtaactctaa actgtctgcc
     8521 gcagtctttg caacgatagt tttgcatccc attttgtaaa ccatttttaa taatgtgatt
     8581 gctacgacag tctgggcata gtggtggggc actgagtatt ttcagacctt gataatcgac
     8641 tacatcggct tctaattttt tcattagaga aaaaaggtta tctaagaatt ctttattctc
     8701 ttccttactc atagcaaggt atttact
//
LOCUS       DAOPRN010000070         8664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_6784_length_8664_cov_117.166338, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000070 DAOPRN010000000
VERSION     DAOPRN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6784_length_8664_cov_117.166338"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..161
                     /locus_tag="PKI65_07235"
                     /pseudo
     CDS             1..161
                     /locus_tag="PKI65_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_053432883.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            515..2863
                     /locus_tag="PKI65_07240"
     CDS             515..2863
                     /locus_tag="PKI65_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78268.1"
                     /translation="MFQYKFFNLTKNKHLVILIIFLNFFLNLNSKGVPIGAWRYHLPF
                     NNVFAVANTGSLYWACNPYYLYNYDFNTNEINIFSRLNKLSDIKLSAFAIDKIHSLII
                     LGYENGNIDIFNYKTEKVRNVPDIFLKPIIGSKTINHIYINGDEAYFSTDFGIVVYDL
                     KKFEFRNTYYLSQYYQNKKIKTTFIFKDTVFSLFDNFIINAPIDGSNLNDFSNWSIFY
                     SDNNNIIFADALKDDLYMVIDSGCNDHILRYHDGLWDTVLDTLNFNINKLKYDNNKFI
                     ILAEGAVFIYDSLWNFKYKIYQYYPTNYAPMPLDATYTEDNKHLLIGDKNVGLLYNWN
                     DTWNNKIITPLGPSTNISFNVSHSNGAILSSSGGYDQSFGNLWRHVNVEVFKNEQWLS
                     YNGDNTPKLNEVRDAIKALTDPMDPDHLFIATYGYGLVEIKNDQVKIYNDTNSVLPST
                     AGITGFCRISTMDFDSDGNLWILVSGANEFTVFKRNGQMIRLPISISISNYNPGAMII
                     DENNKNIYVPFPRGGGGVLVFKYNGTIDNYSDDRSMLLTTQAGQGKLPSSLVYAAALD
                     KKNQLWLATSEGVAVIYNTQNFFTQNPFDASQIILEVGGFAMPMLISERVNDIKIDGA
                     NRKWIATDNAGIFVFSPNGDKEIYHFTSENSPLLSNRVMSLAIDPITGEVFAATELGL
                     VSFHSDAIEPYTYIEELKIFPNPVPADINGYITIDGLPEDSYVTITDSYGKVVYKTKS
                     MGGRATWNGIDVYNNKAKRGVYIVWASNDDGSIKGTGKFFIE"
     gene            2866..3540
                     /locus_tag="PKI65_07245"
     CDS             2866..3540
                     /locus_tag="PKI65_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78269.1"
                     /translation="MNQTTKAFILNLVQHTRKAYIIHSLTPTGRFSFYLASITPKNKS
                     YEFIKTPLINVEITYNDKPSREHLYKVIDITIENFPHMVADHLLHSAIASFMAEIIMK
                     ITPIDLYDTQLYDIVLDFVNKLNNWEKIPNSFLLSFMAYILNYMGILSKKNYSFLDAM
                     PHYESLLNFLEYLYLHPLNSSNDNITLPATPYHLLNLTISYLRAQEILNSDLKSLKVM
                     REVFYR"
     gene            3666..4613
                     /locus_tag="PKI65_07250"
     CDS             3666..4613
                     /locus_tag="PKI65_07250"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOB78270.1"
                     /translation="MVEPKVGIYSGTASRVLAESIANNYGIPIGKTTYTKFADGEFQV
                     SFDETIRGKEVFIVQSTFPPVDNLFELLLMIDAARRASAKHIYAVIPYFGFARQDRKD
                     KPRVAIGSKLIADLLEAAGATHIITMDLHADQIQGFFNIPVDHLFASSIFYDYIKNLN
                     IQNIVVTSPDTGGTKRANIYAKAFNCDMAICYKQREKANQIADMVLIGDVKNKNVIIF
                     DDIVDTAGSLIRAANLMKQKGALSIKAVCTHALLSNNAVEKIEDSVIDQLIVTDTIPL
                     KKSSEKITVLSVAKLFAEVIKRNIQCESISSFFDFNPFL"
     gene            4648..5268
                     /locus_tag="PKI65_07255"
     CDS             4648..5268
                     /locus_tag="PKI65_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOB78271.1"
                     /translation="MKVLSISGSPRESVGKKDAKQLRKKGFVPCVMYGGEKQLFFYVE
                     EIKMLSLISSLEPLMLDIDVNGKIHRCIIQEIQFHPVTDRIIHIDFVEIKDDKPITMN
                     VPVKFEGDSIGIKRGGKLDIKLKKLPVKAIPSKMPSYIPINITKLDIGDSIRVRDISN
                     DDYQILKPPHLMIAKIIFIRTIITQIEEDTDTEELAETDEVANETE"
     gene            complement(5471..6256)
                     /gene="rsmA"
                     /locus_tag="PKI65_07260"
     CDS             complement(5471..6256)
                     /gene="rsmA"
                     /locus_tag="PKI65_07260"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOB78272.1"
                     /translation="MLKNGNFHYKKSMGQHFLKNPEIARHIVDSLQLTTADDVVFEIG
                     PGSGILTQFLIDKFPLLKLIELDREAISLLKENFQNIDKKIIRGNILKIELLDYANRF
                     SIIGNMPYNISGPLLFNALKYSEHLIEWVGMLQLEVVQRILARPKTKEYGKLSVLLQA
                     YFDIKQIMRLSPASFYPPPKVSSAVIRMIPKHTTNIPKTNYDDFVKIVKLSFTHRRKT
                     LRNNLLETLDKEILKDDFFNKRPEELSWQEYDDMIQHFLYKNN"
     gene            complement(6442..8268)
                     /locus_tag="PKI65_07265"
     CDS             complement(6442..8268)
                     /locus_tag="PKI65_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB78273.1"
                     /translation="MKANKTVLTFISTFLLVLFTNAQNIYNDEALALEFFQRGDYENA
                     VDIYERLYYRQGNTYIYDNYLASLIKLNKFKDAEKLIQNQLKNNPSARFYIDLLEVYD
                     LQNDSKKFQKVWNEMLSIASNNETIYLDLASACDFKNRTKWAINILETGVKILPLSHQ
                     INESLAIKYMKIKDYKKNSIHITNLIRNFSNDKDWLIKVLSSILHEDHNDDFSPILKD
                     ILLGFINSNPKSQLYNELLIWYYEQMGAYDAAINQALAFEKRINGEGEMIFDIANELL
                     EIGGTEYAINAFEKLILQFPNSPFSHKAQLLLLNEKMKLILASANIKSINANNIKDEI
                     EIFLSQYPEYRYHPDFMINYSKILCFYLHNCEKALNDLQDLLKRFPVKNFVQASVKFA
                     IADLYLAMDNPWDAILLYGQVEKDFKEDTIGYYAKFYKAYIYYLMGDILFAKAQFDVL
                     KGSTTKFIANDAIQMSVFIQENIGLDSSLVPLQYFAKAEYMEYIHNYERAINYLDTIL
                     LTSPSHPIIDDVFYKKAQIYEKSSLYDKAIVELNKIIDNYYYEVLADDALYRLALIYA
                     DVYQNYEKSKELLLQLITDFPISIYVDMARMKLNKMKNLDSL"
     gene            complement(8432..>8664)
                     /locus_tag="PKI65_07270"
     CDS             complement(8432..>8664)
                     /locus_tag="PKI65_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB78274.1"
                     /translation="PENEYLMVYGKGIKTIDFTIFDRWGNEVFHTTSMEGKWDGTYKG
                     EPALVGDYTYILKVKYFNKPDEILRGHVYLVR"
BASE COUNT         3067 a         1177 c         1242 g         3178 t
ORIGIN      
        1 aagggaccaa aagcaaaaaa gttgttgtaa acaaaacgca gattagacgc ggtgtctata
       61 gtgtacaggt agtcgataag catgtaaacg atttcaaggt ttggttgcga cgatttagag
      121 gagtggctac taaatacctc cagagctatc tgatgtggtt tgtaatcaca cataaatact
      181 taacaagtga gatagaatca gacattggtc ggcttttaaa tctcgcatcc tcggacagat
      241 gggcgtggga tgagtattgg cggctggtga atacgaaata tagataaact acttcaagta
      301 aaacaatgat ttgtttctgg acttttttcg attccttttt ttccaaaact ctattcacat
      361 taaattcgat aaaaaacaat cttgaaataa tgaaaaatac taagattata attttttaag
      421 taacaacata tttattaaaa aaatatatat atttgtaaag tttcaccacg ttgtttatga
      481 aaacttgatt aattttgaaa aattttaatt ttatatgttc caatacaagt tttttaattt
      541 aacaaaaaat aaacatttag ttatacttat aatttttctt aatttttttc ttaatttaaa
      601 ttcgaaaggg gtacctatag gtgcatggcg atatcattta ccatttaata atgtttttgc
      661 tgttgctaat acaggctcat tatattgggc ttgcaatcca tattatttat ataattatga
      721 tttcaatact aatgaaatta atatttttag tagattaaat aaattatctg atattaaatt
      781 atctgctttt gctattgata aaatacactc gctcattatt ttgggatatg aaaacggaaa
      841 tatagatatt tttaattata agacagaaaa agtacgtaat gtacctgata tttttttgaa
      901 acctatcatt ggttctaaaa ctattaatca tatttacatc aatggcgacg aagcttactt
      961 ttcaactgat ttcggcatcg tagtttatga tttgaaaaaa tttgagttta gaaatactta
     1021 ttatctttcg caatattatc aaaataaaaa aataaaaact acttttattt tcaaggacac
     1081 agttttttct ttatttgata attttattat taatgctccg atagatggta gtaatctcaa
     1141 tgatttttct aattggtcca tcttttattc tgacaataat aatataattt ttgctgatgc
     1201 cctaaaagat gatttatata tggtgataga tagtggctgt aatgatcata tattaagata
     1261 tcatgatgga ttatgggata ctgtattaga tactttgaat tttaatatta ataagttgaa
     1321 atatgataat aataaattta ttattttagc tgaaggtgca gtattcatct atgatagctt
     1381 atggaatttt aaatataaaa tttatcaata ttatccaact aattatgctc ctatgccctt
     1441 agatgctact tatacagagg acaataaaca tttattaata ggagataaaa atgtaggttt
     1501 attatataat tggaatgata catggaataa taaaataatt actcccttag gcccttcaac
     1561 taatattagt tttaacgttt cacacagcaa tggtgcgatt ttatcatctt ctgggggtta
     1621 tgatcaatct tttggcaatc tctggcgtca tgtaaatgta gaggtattta aaaatgaaca
     1681 atggctttca tacaatggag ataacactcc taaattaaat gaagtgcgag atgctataaa
     1741 agcattaaca gatcctatgg atcccgatca tttattcatt gctacctatg gttatggatt
     1801 agtagaaatc aaaaatgatc aagttaaaat ttacaatgat accaattcag ttttgccttc
     1861 gacagcaggt ataactggtt tttgtagaat aagcactatg gattttgact ccgatggcaa
     1921 tctatggatt ttggtatcag gtgctaatga atttacagtt tttaaaagaa atggtcaaat
     1981 gattagattg cctattagta tatctattag taattataat cctggtgcga tgattataga
     2041 tgaaaataat aaaaatattt atgtcccttt tcctagaggt ggcggtggtg tattagtttt
     2101 taaatataat ggaacaatag ataactactc agatgataga tctatgctat tgactaccca
     2161 agctggtcaa ggcaaattgc ccagtagttt agtatatgct gcagccttag acaaaaaaaa
     2221 tcaattatgg ttagcaacta gtgaaggtgt tgctgttatt tataatacac aaaacttttt
     2281 tactcaaaac ccttttgatg cttcgcaaat aattttagaa gtaggtggat ttgctatgcc
     2341 aatgcttatc tctgaaaggg taaatgatat taaaatagat ggagctaata gaaaatggat
     2401 tgctactgat aatgctggta tttttgtctt ttctcctaat ggtgataaag aaatatatca
     2461 tttcacttct gaaaattctc ctcttctgag caatagagta atgagcctag cgatagatcc
     2521 tattacaggt gaggtttttg cagctacaga gttaggttta gtatcatttc atagtgatgc
     2581 tatagagcca tatacatata ttgaagaatt aaaaatattc cccaatccag taccagcaga
     2641 tataaatggg tatattacta ttgatggatt accagaagat agttatgtga ctattactga
     2701 ctcatatggt aaagtagttt ataaaacaaa atctatgggg gggagagcca cctggaatgg
     2761 catagatgta tataataata aagctaaaag aggggtttac atagtgtggg caagtaatga
     2821 tgatggctct atcaagggaa ctggtaaatt ttttattgaa taataatgaa tcaaacaact
     2881 aaagctttta ttctgaatct tgtacaacat actcgaaaag cttatattat ccattcactt
     2941 acaccgactg gtagattttc tttttattta gcatctatta ctcctaaaaa taaatcttat
     3001 gaatttataa aaacaccatt aatcaatgtc gaaattactt ataatgacaa gccctcacgt
     3061 gaacatttgt acaaagtaat tgatataact atagaaaatt ttcctcatat ggtagctgat
     3121 cacctattac actctgcgat tgctagtttt atggctgaaa taattatgaa aataacccca
     3181 atagatcttt atgatactca attatatgat attgtattgg attttgtaaa taaacttaat
     3241 aactgggaaa aaataccaaa ttcttttttg ctttctttta tggcttacat actaaattac
     3301 atggggatcc ttagtaaaaa aaattatagc tttttagatg ctatgccaca ttatgaatca
     3361 ctgttaaatt ttttagaata tttatatctt catccattaa attcatctaa tgataatata
     3421 actctgcctg caacaccata tcatttatta aacttgacta tctcttattt acgagctcag
     3481 gagattttaa acagtgatct gaaaagccta aaagtgatga gagaggtttt ttatagataa
     3541 gtatagtaac ttactacaga caactgacaa cacctaattt attatggtgt tttttttatt
     3601 taataaatta aaattataaa attaatttag aataataatt tttttttgta tctttgcaaa
     3661 aaagtattgt ggaacctaag gttggtattt atagtggtac tgcatcacgt gttttagcag
     3721 aaagtattgc aaataattat ggtattccca ttggcaagac tacatataca aaatttgcag
     3781 atggcgaatt tcaagtaagc tttgacgaaa caataagagg taaagaagtt tttatagttc
     3841 aatctacttt tccacccgta gacaatttat ttgaactttt attaatgata gatgctgcca
     3901 gaagggcttc tgccaagcat atctatgctg ttatacctta tttcggtttt gctagacaag
     3961 acagaaaaga taagccacgt gtagctattg gttcaaaact aatcgcagat ttgttagaag
     4021 cagctggagc cactcatata attaccatgg atttacatgc tgatcaaata caaggttttt
     4081 ttaatattcc tgtagatcat ctatttgctt ctagtatatt ttatgattat ataaaaaatc
     4141 ttaatataca aaatattgta gttacttcac ctgatacagg tggtactaaa agggctaaca
     4201 tatatgctaa ggcttttaat tgcgatatgg ctatatgtta taaacaaaga gaaaaagcta
     4261 atcagatagc tgacatggta ttgataggtg atgtaaaaaa taaaaatgtt attatttttg
     4321 atgatatagt ggataccgcc ggatcactca taagagctgc aaatttgatg aaacaaaagg
     4381 gggctttatc tatcaaagca gtttgtactc atgcattatt aagtaataat gcagtggaaa
     4441 aaatagaaga cagcgtgata gatcaactaa tagtaactga tactattcca ctaaaaaaat
     4501 catctgaaaa aataacagtc ctatcagtag caaaattgtt tgctgaggtt atcaaaagaa
     4561 atattcaatg tgaatctatc agttcttttt ttgatttcaa tcctttctta taaataaaat
     4621 atgttaaaca aataaaaata aaaaattatg aaagtgcttt ctattagtgg ctctccgaga
     4681 gagagcgtag ggaagaaaga tgcaaaacaa ttgcgtaaaa aaggctttgt gccttgcgta
     4741 atgtatggtg gcgaaaaaca attattcttt tatgtagaag aaataaaaat gctctcctta
     4801 atttcatccc tcgaaccatt aatgctagat attgatgtta atggtaaaat tcatcgctgt
     4861 ataatacaag agatacagtt tcatccagtc actgatagga taatacatat cgattttgta
     4921 gaaatcaaag atgataagcc tataactatg aatgtacctg taaaatttga aggagactct
     4981 attggcataa agcgcggtgg caaattagat attaaattga aaaaattacc agttaaagct
     5041 attcctagca aaatgccttc ttatattcct attaatatta ctaaattaga tataggtgac
     5101 agtatacgtg tcagagatat cagcaatgat gattatcaaa tattaaaacc tcctcattta
     5161 atgattgcta aaataatatt tattcgtact attattactc aaatcgaaga ggatactgac
     5221 actgaagaac ttgctgaaac cgatgaggtt gccaatgaaa cagaatgagt aattaatatt
     5281 tacttctatg tcattgtgat taattttttc attttatcaa tataaatgtg aaacaaatta
     5341 taatgttatt tgttttgaat ttgaagtatt gaaatttatt ctaaacctaa tattagactg
     5401 ccaaaaatac tgtaaaacta ttttgaatat ttaatttcat ttattcaaag ttatttgatt
     5461 tagaaagttt ttagttattt ttatataaaa aatgctgtat catatcatca tattcttgcc
     5521 agcttaactc ttcaggtctt ttgttaaaaa aatcatcttt taatatttct ttatctaaag
     5581 tctctagcaa attattcctt aaagtttttc ttctatgagt aaaacttaat ttaacgatct
     5641 ttacaaaatc atcataatta gttttgggta tatttgtagt atgtttgggt atcattctga
     5701 taactgcaga agatacttta ggtggtggat aaaatgaagc tggtgataaa cgcattattt
     5761 gttttatatc aaaatatgct tgtaataaca cactcaattt accgtattct tttgttttag
     5821 gacgtgctaa aatcctctgt accacctcta attgtaacat acctacccat tctattaagt
     5881 gttcactata ttttaaagca ttaaaaagta aaggaccaga aatattgtat ggcatattac
     5941 ctatgataga aaaacgattt gcatagtcta ataattctat tttcaatata tttcctctta
     6001 ttattttttt atcaatattt tgaaaatttt cttttagcaa tgatatagct tctctgtcga
     6061 gttctattaa ttttaataaa ggaaatttat caattaaaaa ttgtgtaagt attccactac
     6121 caggaccaat ttcgaaaact acatcatcag cagtggttag ttgtaatgaa tctacaatat
     6181 gtctagctat ttcaggattt tttagaaaat gctgtcccat tgatttttta taatgaaaat
     6241 taccattttt aagcatttga attttttttt aaacaaattt ataaattttg tagaagttag
     6301 ttattggctg aactatacaa tttattgagt tgggatgtat tacatacagt acgtcccttc
     6361 aattcttaat aatctttgat taataatgga acatcatatt aatcttctga gaatttataa
     6421 ttcaaacatt ttatagataa attataaaga atctaaattt ttcattttat tcaatttcat
     6481 tcttgccata tcgacatata tacttatagg aaaatcggta attagttgta aaagaagttc
     6541 ttttgatttt tcataatttt gataaacatc tgcatatatt aaagccagtc tatacaatgc
     6601 atcatccgct aaaacttcat aataataatt atcaatgatt ttattcaatt ctactatagc
     6661 tttatcatat agagatgatt tctcataaat ctgtgctttt ttataaaaaa catcatcgat
     6721 aataggatga ctgggagaag taagtaaaat tgtatctaga taattaatgg cacgttcata
     6781 gttatgaata tattccatat attcagcttt cgcaaaatat tgtaatggta ctaacgagct
     6841 atctaaacca atattttctt ggataaaaac actcatctgt atagcatcat ttgctataaa
     6901 tttagttgta gatcctttga gtacatcgaa ttgagctttt gcaaatagta tatcacccat
     6961 taaataataa atatatgctt tataaaattt ggcataataa ccgatagtat cttccttaaa
     7021 atctttttct acctgtccat atagtaatat agcgtcccag ggattatcca ttgctaaata
     7081 taaatctgca atagcaaatt tcacactagc ttgtacaaaa tttttaactg ggaatctttt
     7141 aagtaaatct tgtaaatcat tcaatgcttt ttcgcaatta tgtaaataaa aacataaaat
     7201 ttttgaataa ttgatcataa aatcaggatg gtaacgatat tcaggatatt gagataaaaa
     7261 tatttcgatt tcatctttaa tattattagc attaatagat ttaatattag cagaagctag
     7321 tattagcttc attttttcat ttaataataa aagttgtgct ttatgtgaaa aaggagaatt
     7381 tggaaattgc aatattaatt tttcaaatgc attaatagca tattcggttc cgccgatctc
     7441 taataattca tttgcaatat caaaaatcat ttctccttcg ccattaattc ttttttcgaa
     7501 agctagtgct tgattaatag ctgcatcata agctcccatt tgctcataat accatatcaa
     7561 aagttcatta tataattgac ttttgggatt agagtttata aaacccaata atatatcttt
     7621 tagtattggt gagaaatcat cattatgatc ctcatgtaga atggaagata atacctttat
     7681 tagccaatct ttgtcatttg agaagtttct tattaaatta gtaatatgaa tagagttttt
     7741 tttataatct tttattttca tgtattttat agccaagctt tcattgattt gatgggatag
     7801 tggtaatatt ttaactcctg tttctaaaat atttatagcc catttagttc tatttttaaa
     7861 atcacaggct gatgccaaat ctaaatatat agtttcatta ttactagcta tacttagcat
     7921 ttcattccat actttttgaa attttttact atcattttgt aaatcataaa cttctaataa
     7981 atcaatataa aatctagcgc taggattgtt ttttaattga ttttgaatta atttttctgc
     8041 atctttaaat ttattaagct tgataaggct agctaaataa ttgtcataaa tgtaagtatt
     8101 cccttgtcta tagtagagcc gttcataaat atcaacagca ttttcatagt cacccctttg
     8161 aaaaaattct aatgccaaag cctcatcatt ataaatgttt tgtgcatttg taaataaaac
     8221 taataaaaaa gttgaaataa aagttaatac agttttattt gctttcattt ttttatttaa
     8281 tataaacaaa attacaaata aatttctcaa caataactaa aatttagatg caaatcttaa
     8341 tatattatac tgatattaga atttacatta aaatctacac aatgaagatt tagaattact
     8401 aatattttgt aaaatattaa ttttaagaaa tttaacgaac tagatataca tgtcccctca
     8461 gaatttcgtc aggtttatta aagtatttaa ctttcaaaat ataagtgtaa tcacctacta
     8521 atgccggttc acctttataa gtaccatccc atttaccttc cattgatgta gtatgaaata
     8581 cttcatttcc ccatctatca aatattgtaa aatctatagt tttaatacct ttaccatata
     8641 ccattaaata ttcattttca ggat
//
LOCUS       DAOPRN010000071         8514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_6928_length_8514_cov_258.665918, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000071 DAOPRN010000000
VERSION     DAOPRN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6928_length_8514_cov_258.665918"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..1308)
                     /locus_tag="PKI65_07275"
     CDS             complement(322..1308)
                     /locus_tag="PKI65_07275"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78275.1"
                     /translation="MDNFNLLDYQALPKEQKRRFLDNLYQFLLENNYTAVDYQKLKIQ
                     STAPICPRCESENVIKAGVRDGKQVYKCKDCGRQYRETARTFVYRMRKADKMLDYLKC
                     MLEGKSLRACASEVGISLRTSFNWRHKILAAIKSLQGGISFSGIVEADELLMKYSEKG
                     RKYRSRKEYEKAKKKKHPKVAVLVMTDREGNLLFKHVGRKKVTNSQIREELSHRVTDS
                     NLICVKQKKEFKKAVKGTKSKKVVVNKTQIRRGVYSVQVVDKHIDDFKVWLRRFRGVA
                     TKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSDRWAWDEYWRLVNTKYR"
     gene            1724..1796
                     /locus_tag="PKI65_07280"
     tRNA            1724..1796
                     /locus_tag="PKI65_07280"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1757..1759,aa:Lys,seq:ttt)
     gene            complement(1914..2171)
                     /locus_tag="PKI65_07285"
     CDS             complement(1914..2171)
                     /locus_tag="PKI65_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78276.1"
                     /translation="MSEILQEIFTSTDLDIRLQVLLEIGKEILTINKPNKFIKIYQID
                     DFYVELIYKNELKKITYINLIDPQEFLEKYNNYNLLNKLMN"
     gene            complement(2178..2582)
                     /locus_tag="PKI65_07290"
     CDS             complement(2178..2582)
                     /locus_tag="PKI65_07290"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOB78277.1"
                     /translation="MMKYKLLIIIMILFSFININGQIIVHKNDILDDLVAKHTRLTAK
                     PNSFFGYTIQLGAFSGNYSKSKAEALMYEVINNISNIPVNIVFDSPYYKVMAGAYIDK
                     YEARYDYEYIRKTYPQAIIVPYFINVKNLIIK"
     gene            2655..3305
                     /locus_tag="PKI65_07295"
     CDS             2655..3305
                     /locus_tag="PKI65_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOB78278.1"
                     /translation="MKTIIKIFVCNPFMVNNYVLRNEKNEALLIDAGYSNNEEWSILH
                     NYIIQEKLDIKACLITHPHIDHIWGINEFYNKYAKDIMMSPEGYQLYQNINDLADQLG
                     MDKIDILDKSKIKFISEGEIKIGDFCIIVLETPGHSDGSLCFYLPEDNIIFSGDLLFY
                     ESVGRTDFITGDWNKLVDSVKNKLYKLPDDTKIYPGHGDPTTIGHEKKNNPFVSEL"
     gene            3650..3724
                     /locus_tag="PKI65_07300"
     tRNA            3650..3724
                     /locus_tag="PKI65_07300"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3685..3687,aa:Pro,seq:tgg)
     gene            3747..3820
                     /locus_tag="PKI65_07305"
     tRNA            3747..3820
                     /locus_tag="PKI65_07305"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3781..3783,aa:Arg,seq:tct)
     gene            3827..4480
                     /locus_tag="PKI65_07310"
     CDS             3827..4480
                     /locus_tag="PKI65_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78279.1"
                     /translation="MKKLFFYLFYFFLINNLFSQQFIGIANLGFNLSQVDGDEIYGFR
                     KFGVNVGPSVMYSFGQKKQWQLILETTFSQKGSYQKYPNTYIDTMKLPYYNLRLNYVE
                     IPLIFLYNDNDIISFGIGLSYSRLTKVKEIEYGKVNKYNTMRGPYDADDLDVLVELRF
                     RLYQKLYFNIRYSYSIDKIRTREFDNGINTWTRNQYNNVLTFRLLWYFNQNIESKKK"
     gene            4628..6526
                     /gene="dnaK"
                     /locus_tag="PKI65_07315"
     CDS             4628..6526
                     /gene="dnaK"
                     /locus_tag="PKI65_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOB78280.1"
                     /translation="MGKIIGIDLGTTNSCVAVVEGNEPIVIANNEGRRTTPSMVAFTE
                     DGEIKVGDPAKRQAIINPTRTVYSIKRFMGRTYDEVQQEINRVTYKVVKGPNNTPRVK
                     IEDREYTPQEISAMILQKMKKTAEDYLGTEITDAVVTVPAYFNDSQRQATKEAGEIAG
                     LNVRRIINEPTAAALAYGLDKKHKEMKVAVYDLGGGTFDISILELGEGVFMVKSTNGN
                     TSLGGDDFDERLVNWLADEFFKEEGVDLRKDPLALQRLREAAERAKMELSSSMMTEIN
                     LPYITTVDNVPKHLVKQITRAKFESLIEDLVKSTIEPCKKALEDANLKTSDIDEVILV
                     GGSTRIPLVQKIVEEFFGKAPSKGVNPDEVVAVGAAIQGAVLTGEVKDVLLLDVTPLS
                     LGIETLGGVFTKLIEANTTIPTRKTEVFTTAADNQTSVEIHVLQGERPLAKDNKTIGR
                     FHLDGIPPAPRGVPQIEVTFDIDANGILNVSAKDKATGKSQSIRIEASTGLSDEEIER
                     MKEEAQRHAEEDRIIKEKIDKVNQADNLIYSTEKQLKEFGDKIPPANRAEIESALNAL
                     KDAHKSQDVIAIDSAMAQLNDAWQKASSYIYQTTDDQADTSDSTTYDESKASGDDNVT
                     DVDYEEVD"
     gene            6903..8294
                     /locus_tag="PKI65_07320"
     CDS             6903..8294
                     /locus_tag="PKI65_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOB78281.1"
                     /translation="MKMNFIKKYALAKMLRYASHQSDESIIKMINIANKYFIKSEKVR
                     WEAEKIRRIFVEHHPGATLAKNAFSRISKQSLNSVIQNFFINAAVIGRQRQEQLRKEL
                     NIWIPWFFVISPTAKCNLTCVGCYAGNYNKEEDLSYELVDRILNEAKDLGIYFITVSG
                     GEPFMWPHLLDIFEKHHDMFFQIYTNGTMITKEIANRLGKLGNAAPAISIEGFEKETD
                     LRRGEGTFKKIMEAMDNLYEAGVPFGFSATPTKNNIDILMSDEFIDLMIDKGCLLGWY
                     FQYVPIGRRPDVSLMATPEQRFELRKRSIEIRNTKPIFIGDFWNDGPYVGGCICGARQ
                     AGYFHINCRGDVEPCVFLQFSVDNIKDKKLIDVIQSPFFKAFQEAQPYRENNNLLTPC
                     ALIDNPHILRDIVTKYNAKPSYNSSMDVINNPEITSFLDNYSKEYQKLVDPIWENELK
                     FKYKTWKQRWTNN"
BASE COUNT         3013 a         1142 c         1393 g         2966 t
ORIGIN      
        1 tctggaggta tttggtaacc actcctctaa atcgtcgcaa ccaaaccttg aaatcattta
       61 tgtgcttatc gactacctgt acactgtata caccgcgtct aattatttaa ttatgctata
      121 aaatacgtaa agtaaaacaa aggatatttt taagtaactc cgctcaataa atcaccttta
      181 tgaagcgtaa tttttacaaa gctaaatttc ttggctctat ttattttaat tggaatagag
      241 attatagaaa ggaaaattat tgtaattgta aaagaaatcg aaaagggtct agaaacaaat
      301 cattgtttta cttgaagtag tttatctata ctttgtattc accagccgcc aatactcatc
      361 ccacgcccat ctgtccgagg atgcgaggtt taaaagccga ccaatgtcag attctatctc
      421 acttgtcaag tatttatgtg tgattacaaa ccacatcaga tagctctgca agtatttggt
      481 agccactcct ctaaatcgtc gcaaccaaac cttgaaatca tctatatgct tatcgactac
      541 ctgtacactg tatacaccgc gtctaatctg tgttttgttt acaacaactt ttttactttt
      601 ggtccctttc acagcttttt tgaattcctt cttttgcttc acacatatta agttgctatc
      661 tgtcactcta tgcgatagct cttctcttat ctgactattg gttacttttt tgcgtcctac
      721 gtgtttgaaa agtaaattgc cttccctgtc tgtcattact agcacagcta ctttgggatg
      781 tttctttttc tttgcttttt cgtattcttt tcggcttctg tatttgcgac ctttttctga
      841 atatttcatt agcagctcat cagcttctac aatgcctgag aagctgatac caccctgtaa
      901 tgatttgatc gctgctagta tcttgtgtcg ccaattgaaa ctcgttctca gacttatgcc
      961 tacttccgat gcgcaagctc ttagactttt gccttctaac atgcatttta ggtaatcgag
     1021 cattttatca gcttttcgca tgcgatatac gaaggttcta gctgtttcgc ggtattgtcg
     1081 gccgcaatct ttgcatttgt acacttgttt gccgtcgcgt actccagctt ttattacatt
     1141 ttcgctttcg catctaggac agatgggggc tgtgctttgt atttttagct tctgataatc
     1201 tacagccgtg tagttgtttt ctaataaaaa ttgatataga ttatctagaa atcggcgttt
     1261 ttgttctttg ggtagagcct gataatctag tagattaaaa ttatccataa ttattttttt
     1321 gtaaaattaa ataaaaatta tttgatggtc atataaaata cgtaaagtaa aacaaaaatg
     1381 aatttgcagg aagctttttt tcgaaaccta ataagaatcg aattttatga tataatatct
     1441 ttgctatata ttttattttg aggtgaatca attaggtgga tatttaatag atttgttatg
     1501 ttttataaag aagtaatttt aatggtaaat atataatttt taattaactt taaatggatg
     1561 caataatacc attttttaat tattttaata aaataattat ctaataacat ttatttaaaa
     1621 tattgctata tattattatg tatattttaa aaaaattaaa aaaaattttg ctagttataa
     1681 aaatagtatt aattttgcaa tcacaatttt tctaaaaaat aatggcaatg tagctcagga
     1741 ggtagagcac atccctttta aggatggggt cccgggttcg aatcccggca ttgtcactta
     1801 gtatagatta ttaattaata aattagaaaa taaactaatt tttcgtttat atatttaaag
     1861 tcttttattt ttatattaat ttactttaat atttacaatg aactaaaaga attttaattc
     1921 attaatttat ttaagagatt ataattgttg tatttttcta aaaattcttg aggatctatt
     1981 aaatttatat aagtgatttt ttttaattca tttttataaa ttaattcaac ataaaaatca
     2041 tctatttggt aaatttttat aaatttatta ggtttattaa tggtaagaat ttctttacct
     2101 atttcgagta aaacttgcaa ccttatatct aaatcagtac ttgtaaaaat ttcttgcaat
     2161 atttcactca ttttctttta tttaataatt aaatttttaa cattaataaa ataaggtact
     2221 ataatggctt gtggatatgt ttttctgata tattcataat catatcttgc ttcatattta
     2281 tcgatataag cacctgccat tactttataa tatggcgagt cgaatacaat atttactggt
     2341 atattagaaa tattattaat gacctcatac attaatgctt cagcttttga tttactataa
     2401 tttcctgaaa aggcacctaa ttgtatagta taaccaaaaa aactatttgg cttagcagtt
     2461 aatctagtat gtttagccac caagtcatct agtatatcat ttttatgtac gattatttgt
     2521 ccatttatat taataaaact gaataatatc attattatta ttaataattt atatttcatc
     2581 atttcttcat cattttttac aaatttaaca attatttttg taatattata agtttttata
     2641 tttttacaaa aaaaatgaag acaataatta agatatttgt atgtaatcct tttatggtca
     2701 ataactatgt tttaagaaat gaaaaaaacg aagctttatt aatagatgca ggttattcaa
     2761 ataatgaaga atggagtatt ttgcataatt atataataca agaaaaatta gatataaaag
     2821 cttgtctaat tactcatcct cacatagatc atatatgggg aataaatgaa ttttataata
     2881 aatatgcaaa agatattatg atgtcgccag agggctatca actttatcaa aatattaatg
     2941 acttagcaga tcagcttggc atggataaaa tagatatatt agataaaagt aaaattaaat
     3001 ttattagtga aggggaaatt aaaattgggg atttttgtat tatcgtatta gaaacacccg
     3061 ggcattcaga tggttcttta tgcttttatt tacctgagga caatataatt tttagtggag
     3121 atttactttt ttatgagagt gtaggtagga cagattttat cacaggcgat tggaataaat
     3181 tagtagatag tgtaaaaaat aaattatata agctacctga tgatacaaaa atatacccag
     3241 ggcacggtga tcctactact ataggacatg agaaaaaaaa taatcctttt gtatctgaat
     3301 tataattttt ttattgtagc taaatttttt actataaatc gcaattacca gaaaaatttt
     3361 aaaaaaaatt aagtagttgt atgaatgaat aattttgaaa aattcagtat aaatgatttg
     3421 taatattaat agaaagctgg taatgtattc atgatgagga tgataaaaac attatagtat
     3481 gaataaatag agaaactagt gaaaatgtag taaaatcaca aatttttgaa gcattgatag
     3541 tatatttatt tttgcaaata attaataaaa ctatatcata tacttaataa gttttttcaa
     3601 atttattttt tttataaaaa ataaatttgt aattttgcaa atcaatatac ggggcatagc
     3661 gtagtccggc tatcgcgcct gctttgggag caggaggtcg caggttcgaa tcctgctgcc
     3721 ccgattttta aattttaaaa aaaataggtc tcgtagctca gtaggataga gcggcagcct
     3781 tctaagctgc gggtcgtggg ttcgaatccc gccgagatca cttacaatga aaaaattatt
     3841 tttttattta ttttattttt ttttaattaa taatctcttt tctcaacaat ttataggtat
     3901 tgctaatctt ggttttaatt tatctcaagt agatggtgat gaaatttatg gttttagaaa
     3961 atttggtgta aatgtgggac ctagtgtaat gtattctttt ggacaaaaaa aacaatggca
     4021 gctcatactc gaaactactt ttagccaaaa aggttcttat cagaaatatc ctaataccta
     4081 tatagatact atgaagcttc cttattataa tttaagatta aattacgtcg aaattccatt
     4141 gattttcctc tataatgata atgatattat cagttttggt atcggactta gttatagtag
     4201 attgacaaaa gttaaagaaa tagaatatgg aaaggtaaat aaatataata ctatgcgcgg
     4261 cccatatgat gcagatgatt tagatgtgct agtagagctt cgatttagat tatatcaaaa
     4321 attatacttt aatataagat attcatatag tatagacaaa ataagaactc gagaatttga
     4381 taatggtatc aatacatgga ccagaaatca atataataat gttctaacat ttagactact
     4441 ttggtatttt aatcaaaata tagaatcaaa aaagaaataa actgcatttc aaataatcat
     4501 agcaaatatt acattcaatt ttataaaaaa agacaatttg tcgtctgagg ttatgaaatt
     4561 atgacaatta cataattgat tttgaattgg tatatatttt gttagattaa aataaaaaat
     4621 attaactatg ggaaaaataa taggtataga tttaggtaca acaaattcat gtgtagcagt
     4681 tgttgaaggt aatgaaccaa tagtaatagc taataatgag ggtaggagaa ctactccatc
     4741 gatggtagct tttacagaag atggtgaaat aaaggtaggt gatcctgcta aaaggcaagc
     4801 aataataaat ccaactcgta ctgtatatag tattaaacgt tttatgggta gaacttatga
     4861 tgaagtacaa caagaaatta atagagttac ttacaaagtt gtaaaaggac ctaataatac
     4921 tccacgtgtt aaaattgaag atcgtgaata tactcctcaa gaaatatcag ctatgatttt
     4981 acaaaaaatg aaaaaaactg ctgaagatta cttgggtaca gaaattactg atgctgtcgt
     5041 tacggtgcca gcttacttca atgattctca gagacaggct accaaagaag ctggagaaat
     5101 agctggtttg aatgtaagac gtataataaa tgaacctact gccgctgcct tagcttatgg
     5161 attagataaa aagcataaag agatgaaagt ggcagtgtat gatcttggtg gcggtacttt
     5221 tgatattagt atattagaac tgggtgaagg agtttttatg gtgaaatcta ctaacggaaa
     5281 tacaagtctc ggtggagatg atttcgatga aagacttgtt aattggttag ctgatgagtt
     5341 ttttaaagaa gaaggcgtag accttagaaa agatcctttg gcattacaaa gattaagaga
     5401 agccgctgaa cgtgctaaaa tggaattgtc ttctagcatg atgaccgaaa tcaacctacc
     5461 atatataaca actgtagata atgtacctaa acatctagta aagcaaataa caagagctaa
     5521 attcgaatct cttatagaag atttagtgaa atcaacaata gaaccatgta aaaaagcatt
     5581 agaagatgct aatctcaaaa cttctgatat tgatgaggtt atccttgttg gtgggtctac
     5641 tcgtattcct ttagtgcaaa aaatagttga agaatttttc ggtaaagctc cttctaaggg
     5701 agtgaatcct gatgaagtag tagctgtagg tgctgctata caaggtgctg tattaactgg
     5761 tgaggtgaaa gatgtgttat tgctcgatgt tactccactt tcattaggta tagaaacact
     5821 tggtggtgta tttactaagt taatcgaagc taatactact atacctacta gaaaaactga
     5881 agtctttact accgctgcag acaatcaaac ttctgttgaa attcacgttt tgcaaggaga
     5941 acgacctcta gccaaagata ataaaactat tggtagattt catttagatg gcataccgcc
     6001 tgctcctcgt ggtgtaccgc aaatagaggt tacattcgat atagatgcta atggcatttt
     6061 aaatgtttct gctaaagata aagctacagg caaaagtcag tctattagaa tagaagctag
     6121 cacgggactc agtgatgagg aaatcgaacg tatgaaagaa gaagctcaac gtcatgctga
     6181 agaagataga ataataaaag aaaaaatcga taaagtgaat caagctgaca acttgatcta
     6241 ttccaccgaa aaacaactta aagaatttgg tgacaagata cctcccgcta atagagcaga
     6301 aatagaaagt gctctcaatg cattaaaaga tgcacataaa tcgcaagatg ttatagctat
     6361 agatagtgct atggctcagc tcaatgatgc ttggcaaaaa gctagctcat atatatatca
     6421 aacaaccgat gatcaagctg atacttctga ttccacgact tatgatgaat ctaaagctag
     6481 tggagatgat aatgtaactg atgttgatta tgaagaagta gattaaaaaa aaattaaata
     6541 ataaaaaata agggcaattt tttataaaaa attgccctta ttttttatta ttatagacct
     6601 aaaattaaat taattatctt atgtaatatt tataaattaa tcatttttat tataactaat
     6661 aaagtgctta aattaggttg caaaatacat actagagctc tattcattga gatattttac
     6721 ttgcttaagt gatttttgaa atttttagta tatatgtaac agtatattag taattcatga
     6781 attaccaatt caatacaaat ctgattttat tatattttat aaatcagatg tcctaattag
     6841 tattttcata atattttttt ttatatttta ttttttatat aaatttgtaa taaaaaaaga
     6901 ttatgaaaat gaattttatc aaaaagtatg cacttgcaaa aatgcttagg tatgcatctc
     6961 atcaatctga tgagagcata ataaaaatga ttaatatagc taataagtac tttatcaaaa
     7021 gtgaaaaagt aagatgggaa gctgaaaaaa taagaagaat ttttgtagaa caccatcctg
     7081 gtgctactct agctaaaaat gcatttagta gaatttctaa acaaagttta aacagtgtta
     7141 ttcagaattt ttttattaat gctgctgtaa taggtagaca aagacaagaa caactaagaa
     7201 aagagttaaa tatctggata ccgtggttct ttgttattag tccaacagct aaatgtaatt
     7261 tgacgtgcgt aggttgttat gctggtaatt ataataaaga agaagatttg tcctatgaac
     7321 ttgtagatcg catattaaat gaagctaaag atttaggtat atattttatc actgtatctg
     7381 gtggagaacc ttttatgtgg cctcacttat tagatatctt tgagaaacat catgatatgt
     7441 tttttcagat atatactaat ggtactatga taacaaaaga aatagctaat agattaggta
     7501 aattggggaa tgcagcacca gctatttcta tagaaggttt cgaaaaagaa actgatttgc
     7561 gtcgtggtga aggtactttt aagaagataa tggaagctat ggataatcta tatgaagctg
     7621 gtgtgccttt tggtttttct gctactccta caaaaaataa tatagatatc ttgatgtctg
     7681 atgaatttat tgatttgatg atagataaag gctgcttact gggttggtat tttcagtatg
     7741 tacccatagg caggcggccc gatgtaagtc tgatggctac tccagagcaa aggtttgaat
     7801 taaggaaaag atctattgaa atacgtaata ctaaacctat ttttattggt gatttttgga
     7861 atgatggacc ttatgtcggt ggatgcatct gtggagctag acaagctggt tattttcata
     7921 taaactgtcg tggagatgta gagccatgtg tatttttaca attctcagta gataatatca
     7981 aagataaaaa attaattgat gttattcagt cgcctttttt caaagctttt caagaagccc
     8041 agccatatag agaaaataat aaccttttaa caccttgtgc attgatagat aatccacata
     8101 tactgagaga tattgtaact aaatataatg ctaaaccatc atataatagc agtatggatg
     8161 taattaataa tccagaaatc acatcttttc tcgataatta ttctaaagaa tatcaaaaac
     8221 tagtagatcc tatttgggaa aatgaattga aattcaaata caaaacatgg aaacaacgtt
     8281 ggacaaataa ctaatttgta gcagtagaaa tatttttacc caaggctaac tgatagcctg
     8341 ctgaaatagc caataaaact gaagtcatag gatgagtaat aggattatta gtaatggcag
     8401 tagccatcag cgataaacca aacaataaat aaaatggatt aatagaaaat ttaatctttt
     8461 tgtactgggg ctacttgttg cgataattgt tgcagtgaat ctgctgcatt ttgc
//
LOCUS       DAOPRN010000072         8450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_6999_length_8450_cov_112.144967, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000072 DAOPRN010000000
VERSION     DAOPRN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6999_length_8450_cov_112.144967"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            682..1233
                     /locus_tag="PKI65_07325"
     CDS             682..1233
                     /locus_tag="PKI65_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOB78282.1"
                     /translation="MKKMNIIALLSLMFFLIFCSPNNKGQIKNKDNNTSYQNDIKANN
                     NATKKIYEIEINNSKINDKPTGKVEYLSTEDFIAKVFDYRINNEWKYRGKVHCVIDFY
                     ADWCAPCRRVSPIMEELAQEYANEIQFYKVNIDYEKEIAQVFGIRSIPAIMICPLNGT
                     PAMAIGALPKQEYQKMISEVIYK"
     gene            1258..1608
                     /gene="trxA"
                     /locus_tag="PKI65_07330"
                     /pseudo
     CDS             1258..1608
                     /gene="trxA"
                     /locus_tag="PKI65_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846417.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            1848..2540
                     /locus_tag="PKI65_07335"
     CDS             1848..2540
                     /locus_tag="PKI65_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB78283.1"
                     /translation="MAEDKKRHKVASEKGPTTYSKIEAFINKNQKNLTYVLIGITAAI
                     LIIWLYSRFVSKPKAIEAASTIYMAEQYYLLDSFNLALNGDGLELGLLDVVDQYGSTP
                     SGKLAHYYIGMIYLKQGNYEDAIAHLKKYKGKDNIISTMAMGAIGDAYLELDEKDIAL
                     KYYKKAVKNSDNNFTAPAYLLRAAITYELMDNYKEALKLYKEIQEKYPRSLEARDIDK
                     YISRAETALMYK"
     gene            2556..3041
                     /gene="ribH"
                     /locus_tag="PKI65_07340"
     CDS             2556..3041
                     /gene="ribH"
                     /locus_tag="PKI65_07340"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOB78284.1"
                     /translation="MNKKINLSKSEANDTLVGSMFRYAIIVSDWNKEITDRLLKGAYE
                     TLLEHGAKAEHIAIKHVPGSYELPQLAQIIAKKQLYDVIICLGSVIQGETRHFEFISD
                     AVANGCMNVALQHSIPVVFGVLTTNDYQQAIERSGGSHGNKGVEAAITAIKMARTKEN
                     L"
     gene            3046..3993
                     /gene="fmt"
                     /locus_tag="PKI65_07345"
     CDS             3046..3993
                     /gene="fmt"
                     /locus_tag="PKI65_07345"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOB78285.1"
                     /translation="MQKANIVFMGTPDFAVPSLELLATHHNILLVVTTPDKPAGRGLK
                     IQYSPIKTKALELQLPVSQPVLLKDPDFINQIKNINPDFIIVVAFRIIPDEIFKIPKL
                     GTINLHPSLLPKYRGPSPIQWAIINGENQTGVTTIFLNEKIDSGPILLQETEPIYDND
                     NFLTLHNRLKIKGAKLLLETINGVIDNTITPKMQDNLVKQNNLYAPKITKEMTKLDLS
                     RSCVYNWRVIRALYPKPAAYIYLVDDNKKKILKILEADYDLRISNPGDFTLLDKHNFA
                     IGCSDGILIPKTVALEGRKVMTSSEFLRGMHFSKIMHFE"
     gene            4021..5616
                     /locus_tag="PKI65_07350"
     CDS             4021..5616
                     /locus_tag="PKI65_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78286.1"
                     /translation="MKKFIVILIIIVSILNVNAQTKTNKATVKFGPEIKVEAAKTLDR
                     IVFATPDQYLYIGNELISLSNKTNIILANAGRDLRFRNILPLTFQGVGDIEELNFLDI
                     CEIKGNIYVFAIIKDKKEKTVSLYMLTLDQEELVASAPRKLGVINYDFTRYANVRNFE
                     LTISQDSSKILVFYPAPYKKGMPERFGAMVFDENMNRIWANEFQLPYPDDYFSVNKIF
                     VGNNGQIFMNCTKYDGNKNAFAERNPKNYHFVLLATDANGQEIKEINIDLKDKYVNDM
                     IAASLPNNDIVCIGFTSPKSLSSKIDGFFNAIIDHNSLDLKQIDQQLFEPSFIAEGYS
                     ASKTKSIETKTDKGKELTLENFYFNDIVVMNDGSVVAFSEQINKNNDKDYIYHYDDII
                     IINYKSNGDLNYIVKIPKRQHLNGIIKSLISYTYSIVNDKIYVLFNDNINNLATPNPT
                     PPKVFTNYPSTSTNNWAVALCTIDGKGNLSKELLFSFKEVDKFYFFPTKSKVMPNKNI
                     ALYLVKVPTLGYQRYKLAIVDFK"
     gene            5749..6624
                     /locus_tag="PKI65_07355"
     CDS             5749..6624
                     /locus_tag="PKI65_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOB78287.1"
                     /translation="MKNPYYNNIVTPIDFNDFTELIIEQTYQIASLFNCDITLLHVLH
                     EVKSIKDNIKEYYDPDEDIIKKMRLLSSIYHKKYEIEFNYKILQGEVSDTIVSFAYEN
                     YARLIVMGKVGRIHINQTKAIGNNTAQVIRKAYCPVLVTSPFGVKKEFNTILFPIDLT
                     HPISQKINAAIQFSRFYLSSLKIVFVMTIKNMNRENEFKQKLEKIRIFMIENNVHCTT
                     SLLANLEPHKTIADEILDYATYSEADMIFLMTQEENEIDWHHTYIGSTATQIIKNATV
                     PVLTVTPFQNTFQVF"
     gene            6640..7332
                     /locus_tag="PKI65_07360"
     CDS             6640..7332
                     /locus_tag="PKI65_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOB78288.1"
                     /translation="MKRSIPNIITLCNGLFGVISIIAILNNKISFVPYFFAIALLCDF
                     LDGMVARLLKTSSALGRELDSLCDVISFGVLPATLIAYMLNFYDNIPNIIIYLSALYP
                     LFTIYRLSIFNVKFYNLSYFVGLSSPMAAVFVITIFFLNQQWFLPWYLYLFINLLIAI
                     FMVLPLHFYNFKDLQKTNLGKVILITILLTIIIFFIIFSWISILIFSIIYLLISLLLE
                     NKKVKSKISTKL"
     gene            complement(7453..8310)
                     /locus_tag="PKI65_07365"
     CDS             complement(7453..8310)
                     /locus_tag="PKI65_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOB78289.1"
                     /translation="MMNKMMNLNELVKKSYNIRKDVINMLARAGSGHLGGSLGMADIF
                     TVLYFSELRHDPNNPKWDLRDRLVLSNGHVAPVWYATLSNAGYFPKEELFTLRQFGSR
                     LQGHPSRLNGLPGIESSSGSLGQGLSIALGMAISAKYLNKSYRIFCLMGDGEMQEGNI
                     WEAIMAAGHYQLDNLLAIVDRNKLQIDGDTEQVMALEPLKEKIIYFNWNVLECNGNDI
                     SSLLECLQLISWSQKPTFIIAHTTMGKGVKSIENNHLWHGKAPNMDEAQKFLIELDEY
                     YKEKYKLLS"
BASE COUNT         3142 a         1134 c         1184 g         2990 t
ORIGIN      
        1 tgtctaagaa ttctttattc tcttccttac tcatagcaag gtatttactg gtattctcaa
       61 cgattttttt catagcctaa aatttttgta aaaatataaa aattatttaa catataaact
      121 tgaaaaactt aaacatttat taatacaatc aatacgtatt gcttattcat ctcgtcccct
      181 taccaatttt acattaccta taccaacaca aaatttacta cttacatttt tataataata
      241 atatcataat ataaataatt ccaattattt taattttgtt agttttttgg ctctttcatc
      301 aacgttattg cagacgtaat caagtgttaa ataaaatgac aaacatattc ttttttttag
      361 aataatttta aaaaagctta ttatgtttat aatatcgttt tatcatttat ttgcaacaat
      421 aaatattcta ttaatccgta taaatttttg attttactgt attttcactc atacatcagt
      481 tttttcatac attcttttcg tctttatctt cattacgaat atgctacaag atttctctat
      541 aaattaaaaa actctcatgt tgatttttca aaaattattc atttctataa gtacttaaat
      601 tttaattttt taaataattt atgataaata tcaaacatta taacaaatta aattagtttt
      661 tctaattttg caaataaaaa aatgaaaaag atgaatataa tagctttatt atcattaatg
      721 tttttcctga tattctgtag tccgaataat aaagggcaaa taaaaaacaa agacaataat
      781 acttcttatc aaaatgatat caaggcaaac aataatgcaa ctaagaaaat atatgagata
      841 gaaataaata attcaaaaat caatgataag ccaactggga aagtagaata tttaagtacc
      901 gaagatttta tagctaaagt atttgattat cgtataaata atgagtggaa gtatagagga
      961 aaggtgcatt gtgtcataga cttttatgca gattggtgtg ccccctgtag gcgcgtgtca
     1021 cctattatgg aggagctagc acaggaatat gctaacgaaa tacaatttta taaagtaaac
     1081 atagattatg aaaaagaaat agcacaggtt tttggtatac gttctatccc tgcaataatg
     1141 atatgccctc ttaatggcac ccctgccatg gctataggtg cattaccaaa acaagaatat
     1201 caaaaaatga tttcagaagt aatttacaaa taataaaaat aaaatattat gatagaacat
     1261 ttgacagcag aaacttttaa agaaaaagtt tttgattatg aaaacgaaaa agaatggaaa
     1321 tataagggtg ataagcctgc tattattgat ttttatgcag actggtgtgg tccttgccgt
     1381 ttgctagcac caatattaga agaaatagat aaagagtatc aaggtaaatt acaaatttat
     1441 aaaatagata ccgaagtaga acaagagctc gcctatgctt ttggtataca aagtattcct
     1501 actatactat ttattccatt agatggacaa cctcaaatag ctgtgggtgc cctgccaaaa
     1561 aatgacttag aaaagattat aagagatgta ttaaacattg aaaaataatt tttttaaaac
     1621 accatttgaa ataaaaactt gtcaaaattt ttatttaagt atggagtatt tatagttaag
     1681 taattaggtt attagttttt gcaagttttt tggattatat ttttatattt atttcaatct
     1741 tatgatttta ttgaaggtag ttagcgagag taagttatca attatttaat gttagttaat
     1801 taaaaaatta attataaatt tgcaaaatat aaatataata aaaaattatg gcagaagata
     1861 aaaaaagaca taaggtagca agtgaaaagg gacctactac ctattctaaa attgaagcat
     1921 ttataaataa aaaccaaaaa aatcttactt atgtattgat aggtatcaca gctgcaatct
     1981 taataatatg gctatatagc agatttgttt ctaaacctaa agccatcgaa gctgcatcta
     2041 ctatatacat ggctgagcaa tattatttat tagattcttt caatttagct cttaatggtg
     2101 atggattaga gcttggttta ttagacgttg tagatcaata tggtagtaca ccctctggta
     2161 aattggctca ttattacata ggaatgattt atttaaagca aggcaactat gaggatgcta
     2221 ttgcacatct aaaaaagtat aaaggcaaag acaacataat atctaccatg gctatggggg
     2281 ctattggtga tgcatattta gaattagatg aaaaagatat tgcgcttaaa tattataaaa
     2341 aagctgtaaa aaacagtgat aataatttca ctgctccagc ttatctttta cgggcagcta
     2401 tcacttatga acttatggat aattataaag aagctttaaa attatataaa gaaattcaag
     2461 agaaatatcc cagatcctta gaagctaggg atatagacaa atatatttct cgggcagaaa
     2521 cagcccttat gtataaataa aattaattta ttataatgaa taaaaaaatt aatttatcta
     2581 aaagtgaagc aaatgatacc ttagtaggat ctatgtttag atatgctatc atagtttcag
     2641 attggaataa agaaattacg gatcgactat taaaaggagc ttatgagaca ctattagagc
     2701 atggagctaa agctgagcat atagcaataa agcatgtacc aggttcttat gaattgccac
     2761 aattagctca aataattgca aaaaagcaac tttatgatgt tattatttgt cttggatccg
     2821 tgatacaagg cgaaacgaga cacttcgaat ttatttcaga tgctgttgct aatggttgta
     2881 tgaatgtagc attacaacat tctattcccg tggttttcgg tgtattaaca actaatgatt
     2941 atcaacaagc catagagagg agtggcggta gccacggtaa taaaggggta gaagctgcta
     3001 tcactgctat aaaaatggct agaacaaaag aaaatttata attaaatgca aaaagccaat
     3061 atagttttta tggggactcc agactttgct gtcccttctt tagaattact ggctacacat
     3121 cataatattt tattggttgt taccactcca gataaacccg ctggtagagg tctcaagatt
     3181 cagtattctc ctattaaaac taaagcttta gaactccaac taccggtatc tcaacctgtg
     3241 ttattaaaag atcctgattt tattaatcaa atcaaaaata tcaatcccga ttttattatc
     3301 gtagtagctt ttaggattat tcctgatgag atatttaaaa ttcctaaatt aggtacaatc
     3361 aatttgcatc cgtctttatt acctaaatat agaggcccct cacctataca gtgggctatt
     3421 attaatggag aaaatcagac tggcgttact actatttttt tgaatgaaaa aatagatagt
     3481 ggtcctatac tattgcaaga gactgaacca atctatgata atgataactt tctaactctt
     3541 cataatcgat taaaaataaa aggagcgaag ctattattgg aaacaataaa tggtgttatc
     3601 gataatacta ttacaccaaa gatgcaagat aatttagtaa aacaaaataa tttatatgca
     3661 cctaagatta ctaaagagat gacaaagtta gatttgtctc ggtcctgtgt ttataattgg
     3721 cgtgtaatac gggctctata tcctaaacca gctgcttata tttaccttgt agatgataat
     3781 aaaaaaaaga tattaaaaat attagaggct gattatgatc ttagaatttc taatcctggt
     3841 gattttactt tgctcgataa gcataatttc gctattggat gtagtgatgg gatactaata
     3901 cctaaaacag ttgcattaga aggcagaaaa gtgatgacta gttctgaatt tttgagaggc
     3961 atgcatttta gtaagattat gcattttgaa taatttttat aaatttgtaa aaaaataatt
     4021 atgaaaaagt ttattgtaat tttaataatt attgtcagta ttttgaatgt taatgcacag
     4081 acaaaaacaa acaaagctac agtaaaattt ggaccagaga taaaagtaga agctgctaaa
     4141 acactagata ggatagtatt tgctacacca gaccaatatt tatatattgg aaatgaattg
     4201 atttctcttt ctaataaaac aaacataatc ttagctaatg cgggtagaga tctgagattt
     4261 agaaatatac tgccactgac ctttcaagga gtaggagata tagaagagct aaatttttta
     4321 gatatatgtg agataaaggg gaatatttat gtttttgcta ttatcaaaga taaaaaagag
     4381 aaaacagtat cattgtatat gttgactctg gatcaagaag aattagtagc ttcagctcca
     4441 agaaaacttg gagtcattaa ttatgatttt actagatatg ctaatgttag aaattttgaa
     4501 ttaactatat cacaagatag ttcaaaaatc ttagtttttt atccagcacc atacaaaaaa
     4561 ggtatgcctg agagatttgg agcaatggtt ttcgatgaaa acatgaatag aatatgggct
     4621 aatgaatttc aactacccta tccagatgat tactttagtg taaataaaat ttttgttggt
     4681 aataatggac aaatatttat gaactgcact aaatatgacg gtaataaaaa tgcttttgca
     4741 gagagaaatc ccaaaaatta tcattttgta cttttagcta ccgatgctaa tggtcaagaa
     4801 ataaaagaaa ttaatatcga tttaaaagat aaatacgtga atgatatgat agctgcttct
     4861 ttgcctaata atgatattgt atgtatcggt ttcactagtc ctaaaagttt atcaagtaaa
     4921 atagatgggt tctttaatgc tataatagat cataattctt tggatctaaa acaaatagat
     4981 caacaacttt tcgaaccaag ttttattgcc gaaggttata gtgcttccaa gactaaatct
     5041 atagaaacta aaaccgacaa aggcaaagaa cttaccttag aaaattttta ttttaatgat
     5101 atagttgtaa tgaatgatgg ctctgtagta gcttttagtg aacaaataaa caaaaataat
     5161 gataaagatt atatatatca ttatgatgat ataattatta taaattacaa atcaaatgga
     5221 gacttaaatt atattgtgaa gatcccaaaa agacagcatt taaacggtat aattaaatct
     5281 ttaatatcat atacatattc tattgtaaat gataaaatat atgttttatt taatgataat
     5341 ataaataatt tagccacccc aaatcctacg ccacctaaag tttttacaaa ttatccatcg
     5401 actagtacta acaattgggc tgtggcttta tgtactatcg acggtaaagg taatctaagt
     5461 aaagaattat tattttcatt taaagaagtt gataaatttt atttttttcc tactaaatct
     5521 aaagtaatgc ctaataaaaa tattgcttta tatttagtga aagttcctac tttaggttat
     5581 caaagatata aattagctat tgtagatttt aagtaaaagg ttgaatttaa gataaaagat
     5641 atatattctt aatcatttgt tgacatttta ttttatcata atgaattgaa ctataattat
     5701 aaaagttaat atttttgtaa tcaagtttta gacaaataaa taactttaat gaaaaatcct
     5761 tactataata atattgttac tccaatagat ttcaatgatt ttacagagtt gataatagag
     5821 caaacttatc aaatagcttc tttatttaac tgtgatatta ctttattaca tgttttacat
     5881 gaagtaaaat ctattaaaga taatatcaaa gaatattatg acccagatga agatataatt
     5941 aaaaaaatga gattgctttc tagtatttat cataaaaaat atgaaataga atttaattac
     6001 aagatattgc aaggagaggt aagtgacaca atagtttcat ttgcttatga gaattatgct
     6061 agattaatag tgatgggtaa agtaggaaga atacacataa accaaacgaa ggctataggc
     6121 aataacactg ctcaagtgat cagaaaagct tattgtccag ttttagtaac atcacctttt
     6181 ggcgtaaaaa aggaatttaa cactattctt ttccctattg atctcactca tcctattagt
     6241 caaaagatca atgctgccat acaatttagt agattttatt taagttcatt aaaaatagtt
     6301 tttgtaatga ctattaaaaa tatgaataga gaaaatgaat ttaaacaaaa attagaaaaa
     6361 ataagaattt ttatgataga aaataatgta cattgtacta ctagtctatt agctaattta
     6421 gaaccacata aaacaattgc cgatgagata ttagattatg ctacttactc agaagctgat
     6481 atgatatttt taatgactca agaagaaaat gagatagatt ggcatcatac ttatataggt
     6541 agtactgcta ctcaaattat aaaaaatgct actgttccag ttctcactgt tacacctttt
     6601 caaaatacat ttcaagtttt ctaatattta ataaataaaa tgaaacgttc aataccaaat
     6661 attataacac tatgtaatgg actttttggt gtcattagta ttatagctat attaaataat
     6721 aaaataagtt ttgtaccata tttttttgct attgcgcttt tgtgcgattt tttagatggt
     6781 atggtagcaa gattgctaaa aaccagttct gcattaggta gagaacttga ttctctatgt
     6841 gatgttatta gttttggtgt tttacctgca actctcatag cttatatgct aaatttttat
     6901 gataatattc ccaatattat tatttatctt agtgctttat atcccttgtt tactatttat
     6961 cgtttgtcaa tatttaatgt taaattttat aatttatcat attttgttgg attatcgtca
     7021 cctatggcag ctgtttttgt aattactata ttttttctta atcaacaatg gttcttacct
     7081 tggtatttat atctatttat taaccttttg atagctattt ttatggtttt acctttacat
     7141 ttttataatt tcaaagattt acaaaagact aatttaggca aagttatttt aattacaatt
     7201 ttattaacaa taataatatt ttttatcatt ttttcgtgga tttctatttt gatattttcg
     7261 ataatttatt tattaatatc attattactg gaaaataaaa aagtaaaaag caaaatttcg
     7321 actaaattat aaaagattta cttttaaaaa tcaaaatgta taaaatcagt aaaatttcat
     7381 atatcaacat gttaatcata ttaaataata tcgaatttca attttttctg ccttgtatat
     7441 ataattatta aattatgaca ataatttata tttttcttta taatattcgt ctaattctat
     7501 caaaaatttt tgagcttcat ccatattagg agccttacca tgccaaaggt gattgttttc
     7561 gatagatttt acaccttttc ccatagtagt atgtgctatt ataaaagtgg gtttttgaga
     7621 ccagctaatt aattgcaagc attctaagag tgatgatata tcattgccat tacattctaa
     7681 cacattccaa ttaaaatata ttattttttc ttttaatggt tccaatgcca ttacttgttc
     7741 tgtatcaccg tctatttgta atttattcct atctactatg gccaataaat tatcaagttg
     7801 ataatgaccg gctgccatta ttgcttccca tatattacct tcttgcattt ctccatcacc
     7861 cattaaacaa aatatcctat aagatttgtt taaatattta gccgatatgg ccattcctaa
     7921 agctattgac aacccctgac caagagaacc acttgatgac tctatgcctg gtaatccatt
     7981 taatctacta ggatgaccct gtaaacgaga accaaactga cgcaatgtaa ataactcttc
     8041 ttttggaaaa tatcctgcat ttgataatgt agcataccac actggtgcta catgaccatt
     8101 agataatacc aatctatctc tcaaatccca tttgggatta ttaggatcat gacgtaactc
     8161 tgaaaaatat aaaactgtga aaatatcagc catacctaat gatccaccta aatgaccact
     8221 acccgctcga gctaacatat taattacatc ttttctgata ttataacttt ttttgactaa
     8281 ttcattcaaa ttcatcattt tattcatcat tttatttttt tattttacaa agataattgt
     8341 ttatttatat tttaaaagaa tcaaaaatta cgatctcaaa gtaatgtatt ttgaatttat
     8401 gttaaattta atattggctt agctctcaag tccctgtata aaaaccacgc
//
LOCUS       DAOPRN010000073         8359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_7099_length_8359_cov_99.835501, whole genome shotgun sequence.
ACCESSION   DAOPRN010000073 DAOPRN010000000
VERSION     DAOPRN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7099_length_8359_cov_99.835501"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..655)
                     /locus_tag="PKI65_07370"
     CDS             complement(119..655)
                     /locus_tag="PKI65_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78290.1"
                     /translation="MKTIKLLFKFFSISLLLVSCNSTFQAKYTYLNKVPVNQAIIAQS
                     TDNDLNNIVINDENKFISHTYNAEEKTTDFENNNILNTKINNNYLNKFKQNNSSKVPL
                     NIFPSISAKHSMSQAKEMNSNMSLNWIVRLLIQVLVLALVLTLLGLLLPSKVFNIVLT
                     VILVIFVIYLIFYLIRWL"
     gene            737..1918
                     /locus_tag="PKI65_07375"
     CDS             737..1918
                     /locus_tag="PKI65_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOB78291.1"
                     /translation="MREDFFRFLAQTTNEPVALEFRLADGCWLEGADGQKYLDFISGI
                     SVSNLGHRPNFILDAVKNQVDTYAHLNVYGEYILSPQVELAKRLASVLPPSLSKVYFV
                     NSGAEAVEGAMKLAKRHTRRYKIVSFKNAYHGSTQGALSLMGNETFKRHFRPLIPNIY
                     HIEMNNYDDLSIIDKYTAAVFMEPIMGEAGIIPAEVDFIKAIRDRCTQVGALLALDEI
                     QTGMGRTGTMFAFEQYDVVPDILLLAKALGGGYPLGAFISSPEIMNSLTYDPPLGHIT
                     TFGGHPVSCAAGLASLDYLICSKIFQEIPEKEDFFITKLLNAIPNLNIRHKGFLIGIE
                     LESSHFVKKVIKGAINDGLIVDWYMFSPQTIRVAPPLIISYDELDLAAEILIKQLKSI
                     L"
     gene            1915..2391
                     /locus_tag="PKI65_07380"
     CDS             1915..2391
                     /locus_tag="PKI65_07380"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOB78292.1"
                     /translation="MNTSDDIYFLDLAIELAVENVKKGGGPFGAVIVKDRKIIAKGTN
                     RVTIDNDPTAHAEIVAIREACKILNDFQLTDCIIYSSCEPCPMCLGAIYWARPKKLIY
                     AADKYDAADAGFDDSFIYDEIKLPYEKRQLKTITIKLEGACKPFDLWKSLENKKIY"
     gene            2472..2987
                     /locus_tag="PKI65_07385"
     CDS             2472..2987
                     /locus_tag="PKI65_07385"
                     /inference="COORDINATES: protein motif:HMM:NF039711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6272 family protein"
                     /protein_id="HOB78293.1"
                     /translation="MKALHFDINKYLDQYYATSEVLVSFKGMITESKIGRLLDELELN
                     LKKNERQLRSSILLVASEMLDNVVKYTSDKILPQVFLAIRKLDTIQIITANTITPIQV
                     DSLRNAVYDANRLSQGKVKEKVELNQAKEKNKKLGLMDVYHKTNNPIQINFYTINDGS
                     IFAEVIATFKI"
     gene            complement(3267..3953)
                     /locus_tag="PKI65_07390"
     CDS             complement(3267..3953)
                     /locus_tag="PKI65_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOB78294.1"
                     /translation="MKYKILLIEDDKHFGYVLKSFLDTKGYDVEWCEDGEIALEKYKQ
                     NPSYHLMLVDIMMPKKDGITLVKEIRAINPSIPIIFITAKSQHEDILTGYEVGADDYV
                     IKPFSIDILLARMQVLLNRVYPKHEVTKLEAGNSILDINRNLLILNKKEEIRLSPIEL
                     EILKTFFLNVNKITSKNELLMHLYGDADYFKSRTLDVYITKIRKWIRQDPYLELISMP
                     RQGYKFISLK"
     gene            complement(3960..5663)
                     /locus_tag="PKI65_07395"
     CDS             complement(3960..5663)
                     /locus_tag="PKI65_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOB78295.1"
                     /translation="MLKQKIRNKEKRWTLTIFIVSGISFIGILLIQYYFISSTINAAH
                     VNFDKAIQESMSKVIYQYEKQEIADLLQTKLSKYKGARVIQTIDSLNKELFKSLEKVG
                     LDTTLSDSVINISRERIRIQIYTNKYGELVQKIDTHIINIEKEQADSATISDLSKKSD
                     LPIAKEETIDSQLDSLVGWVRVPNITLPLKIDSIYQAVDRFLRRSFIISDVMEDFFNI
                     KHFIPVEQRTNAKVLDSLIASALAENNINIKYYFGIYSPSRQSIFLENDSISAQEMIQ
                     KGMMFRLYPSDLFAPPEYLIIYFPNKTSYILSTMSNFLILSLLLILGVLGSFSFILYN
                     LIRQKRLSEMKTDFINNVTHELKTPLATINIACETISNLPNDQLPQMLEIIKEENVRM
                     QTLIEKILQTSLLDKTSIQLNLSKINLHDFLNDLLNDLHLFLKDYNADVTTEFLIKNP
                     EIYADEIHLRQVFYNLIENACKYSNGKPEIKITTRHNNKYIIVEISDKGVGIKKKDLK
                     HIFDKFYRVPTGNIYNTKGYGLGLSYVQAIIEAHKGKIEVISETDQGTIFVLYFKTNT
                     L"
     gene            5911..7005
                     /locus_tag="PKI65_07400"
     CDS             5911..7005
                     /locus_tag="PKI65_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOB78296.1"
                     /translation="MKIHYHSDHLGSASFVTNAEGAAVQHLQYLPYGELFVSQRNTDE
                     FDSRYKFTAKALDNETSYTYFGARYYDSKLSGWLSVDPISDKYPSLSSYLYCANNPVI
                     LRDPNGEFFGIDSWISGFISGFFSTGNNRWGSAWNEANKRMINDFKIWGGLFASDNNK
                     TIGGRMWEVASRFTFQLPQTIAGFATSHCYNTFGLGGGVESVDYLFGATVLKTGNSWG
                     GVTMSNFIIGDKNIKADPSNKLFQHEYSHYLQRQSSGWFYLSKYGIPSLLSKNPHDYN
                     PVEQDANARALRYFSTNISGFNNGDFYYNPILNYNQSLPFNNPINQAALNGANLHLSW
                     YDYFMAPFNLSIIGIPIPGLISTLMLNQKY"
     gene            7010..7459
                     /locus_tag="PKI65_07405"
     CDS             7010..7459
                     /locus_tag="PKI65_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78297.1"
                     /translation="MSIKFKKILKIITYMVVILIFLAIDCIPKNRENCHGTISFKNNS
                     SKTLYITRNYYYTDTTTLIISNPVSSDNTNKVLPFSENKTALWYYGCFEDLLQSLKKD
                     TLFVYVFDAEIVETTPWDTVLANNLILKRYDLSLEDLQNMNWTIIYP"
     gene            7463..7726
                     /locus_tag="PKI65_07410"
     CDS             7463..7726
                     /locus_tag="PKI65_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOB78298.1"
                     /translation="MKSPIFYLYSKFDTRYKFTAKELDNETSYTYFGARYYDSDLSIW
                     LSVDPMSDKYPSTSVYMYCSGNPVIFIDPNGLDEWEVNKKGLI"
     gene            7801..8262
                     /locus_tag="PKI65_07415"
     CDS             7801..8262
                     /locus_tag="PKI65_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78299.1"
                     /translation="MNSPLFANYQAGYDFYENNKFSYTPDRYNGLCRNLGISGKYEIR
                     GDSIYFIVLYISECIGDSIKRSPPSTNKRSEMELYGISSEKSDYRPIPSTSNYWQLSN
                     CKTQRITLEKPETWSASFRYYKDKSGQEVIEIDGDKFYYIPGAEEFGEQEE"
BASE COUNT         2918 a         1106 c         1309 g         3026 t
ORIGIN      
        1 ataatgctaa tggagtaaat gactttactg gtttcatttt ttattacaaa attataaaat
       61 tattactatg tttaaacagt tttttgagtt tgctaataaa attattatta taaaaaaact
      121 atagccatct aattaaatag aaaatgagat aaataacaaa aatgacgagt ataactgtca
      181 aaacaatatt aaaaacttta ctaggtaaca aaagacccaa taaggtaagg actagagcta
      241 aaactaatac ttgtattaaa aggcgcacga tccagttaag cgacatattg ctattcattt
      301 cttttgcctg cgacatagaa tgcttcgctg agatagaagg aaaaatattt aatggtactt
      361 tacttgaatt attttgtttg aatttattta aataattatt attgatcttg gtattgagaa
      421 tattattatt ttcaaaatcc gtagtttttt cttctgcatt atatgtgtgt gatataaatt
      481 tattttcatc atttataaca atattattga ggtcattatc tgttgattgt gctatgattg
      541 cttgatttac aggtacttta ttcaaatatg tatatttagc ttgaaatgtt gagttacaac
      601 taactaataa taaagaaatt gaaaaaaatt taaataataa tttgattgtt ttcataattt
      661 tttgacaaat atataaaaat ttttaaaaac aaatttttat taaatttgca aaagttatta
      721 gttaagttta atagtaatga gagaagattt ttttcgtttt ttagctcaga ctactaatga
      781 acctgtagct ttagaattta gattggctga tggatgttgg ttagaggggg cagatgggca
      841 aaagtattta gactttatat ctggaatttc agtttctaat ctaggtcatc ggccaaattt
      901 tatattagat gctgtaaaaa atcaagtcga tacatatgct cacttaaatg tttatggaga
      961 gtatatttta tctccacagg tagagttagc taagagactt gcaagtgtgt tgccaccttc
     1021 attatcaaag gtatattttg tcaatagtgg tgctgaagct gtagagggag cgatgaaatt
     1081 agcaaaaagg catactagaa ggtacaaaat agtaagtttc aaaaatgcat atcatggttc
     1141 tacacaaggt gccctgtcac tgatgggtaa tgaaacattt aaacgtcatt tcagaccttt
     1201 aatacccaat atttatcata tagaaatgaa taattatgac gatctttcta tcatagataa
     1261 atatactgct gcagtattta tggaacctat tatgggtgag gctggtatta taccagcaga
     1321 ggtagatttt atcaaagcaa ttagagatag gtgtacgcaa gtaggtgctc ttttagctct
     1381 agatgagata cagactggta tgggacgtac aggcactatg tttgcctttg aacaatatga
     1441 tgtggtgcca gatatacttt tattagcaaa agctctaggt ggtggatatc ccttgggggc
     1501 atttatttcc tctccagaga ttatgaattc tctaacttat gatccacctt taggtcatat
     1561 aactaccttt ggagggcatc ctgttagctg tgctgctggt cttgcttcac ttgattattt
     1621 gatttgttct aaaatatttc aagaaatacc tgaaaaagaa gattttttta ttactaaatt
     1681 gctaaatgct attccaaatt taaatattcg acacaaaggt tttttgatag gtatagaact
     1741 cgaatcatct cattttgtaa aaaaagtaat aaaaggcgct ataaatgatg gtttgatagt
     1801 agactggtac atgttttcgc cacaaactat tagagtggct cctcctttaa taatatcgta
     1861 tgatgaatta gacttagcag cagaaatatt aataaaacaa ttgaaaagta tattatgaat
     1921 acaagtgatg atatttattt tttagattta gcaatagaat tagctgtaga aaatgtaaaa
     1981 aagggtgggg gccccttcgg agctgtaata gtaaaagata gaaaaataat tgctaaaggc
     2041 actaatagag ttactataga taatgatcct acagctcacg ctgaaatagt agctattaga
     2101 gaagcatgta aaatattaaa tgattttcaa ttgacagatt gtattattta tagtagttgt
     2161 gagccttgtc ctatgtgctt aggggctatc tattgggcaa gacccaaaaa gctaatttat
     2221 gctgctgata aatatgatgc agctgatgct ggttttgatg attcttttat ttatgacgaa
     2281 ataaaattac cctacgaaaa aaggcagctc aaaactatta ctatcaaatt agaaggagct
     2341 tgtaaacctt ttgatttatg gaaatcattg gagaacaaaa aaatatacta aattaattga
     2401 aaaaaaatta agataaaatt tggtttataa taaataaatt aataactttg caactataaa
     2461 tttaataaat tatgaaagcc ttacattttg atattaataa gtatttagat cagtattatg
     2521 ctacttctga ggtactagta tctttcaaag gaatgattac ggagagcaaa attggtagat
     2581 tattagatga gttagaatta aacttgaaaa aaaatgaacg tcagctacgc tcttccattt
     2641 tattagttgc tagtgaaatg ttagataatg tagtgaaata tacatctgat aaaattttac
     2701 cccaagtttt tctagctatt cgtaaattgg ataccataca aataatcact gcaaatacca
     2761 ttacaccaat acaagtagat tctttgagaa atgctgtata tgatgctaat agattgagtc
     2821 agggaaaagt aaaggaaaaa gtagagctta atcaagccaa agaaaagaat aaaaaattag
     2881 gattaatgga tgtatatcac aagactaata atcctattca aataaatttt tatactatta
     2941 atgatggtag tatttttgct gaagtaatag ctacatttaa gatataaaaa aaaattattc
     3001 atttctgttt tataatttat taatatctca gtgaccaaag ttaattttgt tgtttataca
     3061 aattttaata ggtatatgag atttttcaaa atagttacat ttcaaacaat taattaataa
     3121 aatctaagtt tataattcac aaaactaatt tcataggtta aagtagcaaa ttgttattcc
     3181 ttaacaaatt aacacaaata aaggcaaaag ataaaaattg agtagttgag cagtacagtt
     3241 gttgttcgtt gccagttata aatatattac tttaagctaa taaatttgta tccttgtcta
     3301 ggcatgctta ttaattctaa ataaggatct tgtctaatcc attttctaat ttttgtaata
     3361 taaacatcta aggtacgtga tttgaaataa tcagcgtcac catagagatg cattaatagt
     3421 tcgtttttag aagtaatttt atttacattt agaaaaaaag tttttaatat ttcaagttct
     3481 ataggcgata atcgtatttc ctctttttta tttagtatca ataaatttct atttatatct
     3541 aatatagaat tgccagcttc gagtttggta acctcatgct tagggtatac tctattaagc
     3601 aaaacttgca tacgtgccaa taatatatct atagaaaatg gtttgatgac gtaatcatca
     3661 gcacctactt cgtatcctgt cagaatatct tcatgttgac ttttagctgt aataaaaata
     3721 atcggtatgg atggattgat agctcgtatt tcttttacta atgttattcc atctttttta
     3781 ggcatcatta tatctacaag cattaggtga taagatggat tttgtttata tttttctaat
     3841 gctatctctc catcttcaca ccattctaca tcatatcctt tagtatctaa aaaacttttt
     3901 aatacgtatc caaaatgttt gtcatcttct attaataata ttttatattt cataattcat
     3961 cataatgtat ttgttttgaa gtataataca aaaattgtgc cttgatcagt ttcactaatt
     4021 acttctattt tacctttgtg agcttctata atagcttgta catagcttaa gcctagacca
     4081 tatccctttg tattatagat attaccagta ggcactctgt aaaatttatc aaaaatatgt
     4141 ttaagatctt tcttttttat tcctactccc ttatcagaga tttcaactat tatatattta
     4201 ttattatgcc ttgtagttat ttttatttca ggtttgccat tagaatattt acaagcattt
     4261 tctattagat tataaaatac ctgccttaga tgaatttcat cagcatagat ttctggattt
     4321 tttattaaaa attcggtagt tacatcagca ttataatctt ttaaaaataa atgtaaatca
     4381 tttaataaat cattcaaaaa atcgtgtaga ttgattttgg ataaatttag ttgtatagag
     4441 gttttatcta ataaagaagt ttgtaaaatt ttttctatca aggtttgcat tcttacattt
     4501 tcttccttta ttatttctaa catctgtggt agttggtcgt ttggtagatt actaatagtc
     4561 tcacatgcta tatttattgt agctaatggt gtttttagct catgagtaac attattaata
     4621 aaatcagttt tcatttccga caagcgtttt tgtctgataa gattgtaaag aataaatgaa
     4681 aatgacccta aaacacctaa tataagtaat aaagaaagta tcaaaaaatt gctcatagta
     4741 gacaatatat agctagtttt attaggaaaa tatataataa gatattctgg tggagcaaat
     4801 aaatcactag gataaaggcg aaacatcata cctttttgta tcatttcttg tgcacttatg
     4861 gaatcatttt ctaaaaatat tgattgtcta ctaggactgt agataccaaa ataatattta
     4921 atattaatat tattttctgc taatgcacta gctatgagtg aatctaatac ttttgcattt
     4981 gttctttgtt ctacgggtat gaaatgtttg atattaaaaa aatcttccat tacatcgcta
     5041 attataaaac tgcgtcgtag aaatctatct actgcttgat atattgagtc tattttcaag
     5101 ggcagggtaa tattaggcac tctaacccaa ccgactaaac tatctaattg gctatctatt
     5161 gtttcttctt tagcaatagg taaatctgat ttttttgata aatctgatat agttgcacta
     5221 tcggcttgct ctttttcaat atttattata tgtgtatcta tcttttgtac tagctctcca
     5281 tatttatttg tatatatttg tattctaatt cgctctctac taatattaat tacactgtct
     5341 gacaatgtag tgtctaagcc tactttttct aaagatttaa ataattcctt attgagagaa
     5401 tctattgttt gaattactcg agcacctttg tacttgctaa gtttggtttg taataaatct
     5461 gctatttctt gcttctcgta ttgatatata acttttgaca tagactcttg tatagcctta
     5521 tcgaaattaa catgtgctgc atttattgta gatgatataa aataatattg tatcaataag
     5581 atacctataa atgatatacc tgaaactata aaaatcgtta atgtccaacg cttttctttg
     5641 tttcttattt tttgttttaa cattttttaa ccttatttaa caaaaattaa ctttttatat
     5701 ttaaataatt attttacttt gcattgagat tttatatata tccattttgg agccttgata
     5761 ataaatattt ttagggtttg acgtagcggc tcatcatttt tttgatgagt cgcttttttg
     5821 ttctgtaaaa ttacatataa agtttgtaat aaataaattt tatttaatat tcaaaaatat
     5881 tcattaattt tacctaacaa aaatttataa atgaaaatcc actaccactc cgaccatctc
     5941 ggctccgcct ctttcgtaac taatgcagaa ggtgcagcag tgcagcattt gcagtatctc
     6001 ccctacggcg aactattcgt ctcccagcga aacactgatg agtttgatag taggtacaaa
     6061 ttcaccgcca aggcgctaga caacgagacc agctacacgt acttcggcgc gagatactat
     6121 gatagcaagc tgagtgggtg gctgagcgta gatcctatta gcgataagta tccgagttta
     6181 agttcatatt tatattgtgc aaataatcct gttattttac gagatcctaa tggtgaattt
     6241 tttggtatag attcttggat atcaggattt atatctggat tttttagtac aggtaataac
     6301 agatggggat ctgcatggaa tgaagcaaat aaacgtatga ttaatgattt taagatatgg
     6361 ggaggattat ttgcaagtga taataataaa actataggtg gtagaatgtg ggaagttgca
     6421 tcaagattta cttttcaatt accacaaact attgctggtt ttgcaacatc acactgttat
     6481 aatacctttg gattaggagg tggtgtcgaa tcagtagatt atttgtttgg agctactgta
     6541 ttaaaaacag gaaactcttg gggtggtgtt acaatgagta attttattat tggtgataaa
     6601 aacataaaag ctgatcctag taataaatta tttcagcatg aatatagtca ttatcttcag
     6661 cgtcagtcat caggctggtt ttatctatct aaatatggaa tacctagctt attgagtaaa
     6721 aatccacatg attataatcc agttgaacaa gatgctaatg cacgtgcctt gaggtatttt
     6781 tcaactaata taagtggatt taataatggg gatttttatt ataatccaat tttaaattat
     6841 aatcaatcat taccttttaa taatccaata aatcaagctg ctttaaatgg agcaaattta
     6901 cacttatctt ggtatgatta ttttatggca ccatttaatt tatcaattat tggtattccc
     6961 atacctggat taataagtac attaatgtta aaccaaaaat attagaatta tgagtataaa
     7021 atttaaaaaa attttaaaga tcattacata tatggtagta atacttatat tcttagcaat
     7081 tgattgtata ccaaaaaata gagaaaactg tcatggtact atatcattca aaaataattc
     7141 ttcaaaaact ttatatataa cacgtaatta ttattatacg gatactacaa ctttaataat
     7201 aagtaatcct gttagcagcg ataatacaaa taaagtatta ccatttagtg aaaacaaaac
     7261 tgctttatgg tattatggtt gttttgaaga tttacttcaa agcttgaaga aagatacact
     7321 ttttgtttat gtatttgatg ctgaaatagt agaaacgaca ccttgggata ctgtactggc
     7381 aaataattta attctgaaac gttatgattt aagtttagaa gatttacaaa atatgaattg
     7441 gacaataatt tatccataaa atatgaaatc acctatattt tatctttatt ccaagtttga
     7501 tacccgctat aaattcaccg ccaaagagct agacaatgag accagctaca cctacttcgg
     7561 agcgagatac tatgatagtg acttgtcaat atggctgagc gtagatccga tgagcgataa
     7621 gtatccgagc acgtcggtgt atatgtattg tagtggaaat ccagtgatat ttattgatcc
     7681 gaatggattg gatgagtggg aggttaataa aaaaggttta atatgaaact aataatttta
     7741 tttttattat gtatatgtgc gtttacatgt aacgctcaaa atattgaagg atattggcaa
     7801 ttgaacagtc cattatttgc aaattatcaa gctggttatg atttttatga gaataataaa
     7861 ttttcatata caccagacag atataatggt ttatgtagaa acttgggtat aagcggaaaa
     7921 tatgaaataa ggggagactc gatttatttt atcgttttat atattagtga atgtatagga
     7981 gactccataa aacgcagtcc accttcaact aataagaggt cagaaatgga gctttatggg
     8041 atatcatctg agaaatcaga ttaccgcccc attccttcaa ccagtaatta ctggcagttg
     8101 agtaactgca aaactcaaag aataacactt gaaaagcctg aaacatggtc agcaagtttt
     8161 agatattata aagataaatc aggacaagaa gtcatcgaaa tagacgggga taaattttat
     8221 tatattccag gtgcagaaga atttggagaa caagaagaat aaatagttat attattgaaa
     8281 ttctatgatt taaaaaaaat taatactttt acctcaaata gatataataa ataccaaaaa
     8341 ttaatgcaat gaaaaaagt
//
LOCUS       DAOPRN010000074         7937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_7592_length_7937_cov_100.963207, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000074 DAOPRN010000000
VERSION     DAOPRN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7592_length_7937_cov_100.963207"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..637
                     /locus_tag="PKI65_07420"
     CDS             164..637
                     /locus_tag="PKI65_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78300.1"
                     /translation="MKKLGILVLVAMIGVTFSSCKKETPQSVAEKFLSAYVKADFETA
                     KTVATGDALDQINQLVNLVSTNPEFFPSNLSYENLNCQEMGGRVKCTYTMVEADQRTE
                     GYYIDLEKVNGKWMITALGYEGEEMPEMIEEELPTDTTAVDELAEEMTEEASEQI"
     gene            673..1134
                     /locus_tag="PKI65_07425"
     CDS             673..1134
                     /locus_tag="PKI65_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HOB78301.1"
                     /translation="MKKTLVIVLSVLAILFVQCKKANTPEQVTETFIKALQQNDFELA
                     KSLSDSVALQTLEMIESFASQMGESIVKDTSLPTDVENIKCEDMQGTNMKKCYFTTQG
                     SESYIELQKRGKEWKVVNFSKEGAEPSEEPLLEDIDLTDTTDTTIDTISIQ"
     gene            1355..1906
                     /locus_tag="PKI65_07430"
     CDS             1355..1906
                     /locus_tag="PKI65_07430"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOB78302.1"
                     /translation="MIIDNLIIDNELFNTYFACNLDKCHGICCVEGVAGAPLENQEID
                     WLQNNVNNFHKYLDKKGKEAIEKIGVYEIDLQGDLVTPLKAKGECAYLIWENGCAKCV
                     FQKLYNEGKLNFYKPISCHLYPIRVYKLVGFYHLTLHKWYTCTAAFEHGKHEKIPVLL
                     FLKDALRRKFPNQVEKILEKTNY"
     gene            complement(2352..3461)
                     /locus_tag="PKI65_07435"
     CDS             complement(2352..3461)
                     /locus_tag="PKI65_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOB78303.1"
                     /translation="MKNQEIGGKSIENKFLTFLYSLDDNFNKNKKIEEIFTYIINNLC
                     VNFSYPDRCNVSIVYKDAYYGSNIDDVLDPDDILTFPLIINKKENGNINVYYEGSENE
                     KLKFTNSERDFLKLIATRLSDFITISEIMENHGYKEYTPIYDAKIVEKHSLDLVNWLY
                     SFNLTMDQIEKLLHTCLNFRKGETVCKQNAFSSYFLLLADGYCKSFVEDSKGHTLSFM
                     VNKAFEFIALSSLYCKSFYFTTMTLTPCKIYLIEKNDFLEIMKYNQSFHTEVTKWLCD
                     NYNIVFEKLAIRGLKQTIGRVAHTLLYLSQTVFENNEIPNIISRKDIAEFSGMSTENC
                     VRILSSLKKDNIIKYTKKGIQIVDLDLLKTIVIAG"
     gene            complement(3586..5421)
                     /locus_tag="PKI65_07440"
     CDS             complement(3586..5421)
                     /locus_tag="PKI65_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB78304.1"
                     /translation="MKKKSLLVLMIVAGFSMSLFAQGRPQVPAYLKNYAVTAQYNKQF
                     KGDEPVPQVNIPYAPMNEKIFHNQPIGNTEYDIQSNKSIDSRIYLYPDGTIGATWIRG
                     TGTLADRGTAYNYYDGTAWGTQISRIETVRTGWGSYYPFKNGEIVISHSGATDLVMNK
                     REAKGAGNWSQTYIAAPTGLEMTWPRVVTVGDTIHVLSAHGGDNPYQGVGTAIVYSRS
                     TDGGNNWTHSVLPGMDADAEQLSYSADIYAWAKPKNGTLAFIVGNMWHDVFIMKSTDG
                     GDTWTKTVVFEHPSPFTFDTGVPLDTNYVCDGLVALEMDNSGKLHATFGVVRIMVEEP
                     STEQFSWFPFTSYLAYWNEDMGTIYDLDMETTEIGIIGWLMDLDNSGIIFDNWTSGAW
                     EQVPTYGNHGLVSQPQITIDDDNNIFVTFAHLNENQFSYAFYRHIWATKSTNGGDDWS
                     EFSELTGGDDYDLIECVYGAMAKNTDDYIHLIFQADGGPGNALQDESHTQWENEIIYM
                     KVLKSDIGVTSYDIHEYNTINSVTVYPNPASDYVNVNIVSPISTNAVITISNILGQQV
                     YAKSIDVKVGSTLVPINVNNLPAGLYMISLQADGKYLNTQKIIIE"
     gene            complement(5579..7510)
                     /locus_tag="PKI65_07445"
     CDS             complement(5579..7510)
                     /locus_tag="PKI65_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB78305.1"
                     /translation="MKRKKSFLVLVLVVSVSLLMAQNKGVNIAPKKVEPNAINKALQN
                     PNGQVSFASYKNEIATILDQLSQKHNKRAISPSHMGHSINIFSVILPEQTCLSANQDL
                     NLIAFTYRQDPSYDGGSGVLRTSFSTDGGSSFDSTTLIPWSLPISTGNYLARYPSGVI
                     YNPNNNSDYENAAIIVAGPMLIGGSWGGDFYGSEFFNGTGTNNQILPYDDNIYPTTSN
                     PSLFNRLFMQSRGNKFFVLGDAHTDDGTYYTSFKTVVNIGEWDTSGDSVIWKRVNVVP
                     DFAITDGLPKGYSTAALAMADDGTNGYLVYSGRNPDAYDPLSYQPIIYETSDGGNTWD
                     KITFNWGGISTIEDHADYYSRVGRPFFGYPLEAMIDKNGHLHFACYIHGAWSDHPDSL
                     SYYANRYYMKGIVFDIFQTSSGWDAAVLDTVWCDDFLFYEEGGSKYYIDDRFQMSRTP
                     DGKYIVFAWADSDTTWVTTTDNEFDNMLPNIFIKVYDADNNTYSQTHNVTRNTYVEDQ
                     ATWFYLADQCFDLGNGNIKVPLSVGFLGGNYTSDDRIDYYFLNDVIIGPAGINNYTNS
                     ANIYIYPNPVSDQLNINLIDKGANFIKILNILGAEVYSTVIEGKDHCTIDLSDLSAGI
                     YIISISNSTSNTAKKFIKK"
BASE COUNT         2885 a         1232 c         1058 g         2762 t
ORIGIN      
        1 aaatttttca aattgctttg agcaaaattt aatattttaa taataatata aaaattacct
       61 taactactta ttcaataatg taaaagtaat tctataaaaa atttttttat ataaaaaata
      121 ttttataatt ttgcaatgtc aaaccctaaa aacaaaaaaa gatatgaaaa aattaggaat
      181 tttagtattg gtagcaatga ttggtgttac ctttagtagc tgtaaaaaag aaacacctca
      241 gagtgtagca gaaaaatttt tatcagctta tgtaaaagca gattttgaaa cagctaaaac
      301 agtagctaca ggagatgctt tagatcaaat caatcaatta gttaacttag ttagtacaaa
      361 tcctgaattc ttcccatcaa atttaagtta tgaaaactta aattgccaag aaatgggtgg
      421 tagagttaaa tgtacttaca ctatggtaga agctgatcaa agaactgaag gatactatat
      481 agacttagaa aaggtgaatg gtaaatggat gatcaccgca ttaggctatg aaggtgaaga
      541 aatgcctgaa atgatcgaag aagaattacc tactgataca actgcagtag atgaactagc
      601 tgaagaaatg accgaagaag cttctgaaca aatctaaaaa agtaaagtaa ttaataaaaa
      661 ttttatctaa ttatgaaaaa aacattagtt atagtattgt cggtacttgc tatactcttc
      721 gtacaatgta aaaaagctaa tacacctgag caagtaacag aaacttttat caaagcttta
      781 cagcaaaatg attttgaatt agctaaatct ctatctgata gcgttgcttt gcaaacactg
      841 gaaatgatag aaagctttgc tagtcaaatg ggagaatcta tcgtaaaaga tacttctctg
      901 ccgacagatg ttgaaaatat taaatgtgaa gatatgcaag gaactaacat gaaaaaatgt
      961 tactttacta cacaaggttc tgaaagttat atcgaattac aaaaaagagg taaagaatgg
     1021 aaagttgtca atttttctaa agaaggtgct gaaccctctg aagaacctct tttagaagat
     1081 atagatctaa cagataccac tgatactact attgatacaa taagtattca ataaaattat
     1141 tttattcgca aaaaagggca ctttattagt gccctttttt attttattta taccaacaaa
     1201 aataatatta aacattgata caaaaaatga ttataaactg cgaaaaaaaa gttgagaagt
     1261 agagagagtg gtaaatagtt aattgaaaaa tctaaactta tatgttaatt ttatcaataa
     1321 attttcacta attttgttat cataaaattt taaaatgata atagataatc taataataga
     1381 taatgagttg tttaatactt attttgcatg caatctcgat aaatgtcatg gtatatgctg
     1441 tgtagaaggt gtagcaggag ctcctttaga gaatcaagaa atagattggt tgcaaaataa
     1501 tgtaaacaat tttcataaat atttagacaa aaaaggcaaa gaagccattg aaaaaattgg
     1561 tgtttatgaa atagatcttc aaggtgatct ggtaactcct ttaaaagcaa aaggtgaatg
     1621 cgcatattta atctgggaaa atggctgtgc taaatgtgtt tttcaaaaat tatataatga
     1681 aggtaaattg aatttttata agcctatctc ttgtcattta tatcctatta gagtatacaa
     1741 attagtaggc ttctatcatt taacattgca taaatggtat acttgtactg cggctttcga
     1801 gcatggcaag cacgaaaaaa taccagttct acttttttta aaagatgctc ttagaagaaa
     1861 atttcctaat caagttgaaa aaatcctaga aaaaacaaat tattaatcag aaatacatat
     1921 aaattcgaaa taataaacaa acaatatttt ttggttctat aacgtttatt atgtgtaata
     1981 aaattttaga ttgttacaaa aaaataacta ttttttaatt attttagtta ttttttacca
     2041 cagagaacac caagttatta cacaaagtcc acaaagtgcc atttatagtc cttttaattt
     2101 cattcttctc tgagttcttt gtgcattctt tgagaacttt gcggttaata tttaactgtc
     2161 caataataaa taaaacaata ttaacaaatt catttttgta caattactct ttttttaatc
     2221 tttacctatg taaaacgtta tagaacctaa ttttttaaaa tcattaagta tttaaaaatt
     2281 tgcaaataat taatagtaga gtttaaggtt aaatactaaa aactgtcaaa ctatctcata
     2341 cattacatat actaaccagc tataactata gtttttaata aatccaaatc tacaatttgt
     2401 attccttttt tagtatattt aataatatta tcttttttaa gcgaagataa tattcttaca
     2461 caattttcag tagacatacc agaaaattca gcaatatctt tgcgggaaat tatatttggt
     2521 atttcattat tttcaaaaac agtttgactt aaatatagta gtgtgtgtgc cactctgcca
     2581 atagtttgct tgagacctct aatagctaat ttttcgaaaa caatgttata attgtcacat
     2641 aaccatttag taacttcggt atgaaatgat tgattgtatt tcattatttc taaaaaatca
     2701 tttttttcga ttaaatatat tttacatggt gtcaaagtca ttgttgtgaa ataaaaactt
     2761 ttacaatata aagaagataa agctataaat tcgaaagctt tattaaccat aaaacttaat
     2821 gtatgtcctt tgctatcttc tacaaaagac ttacaatatc catctgccaa gagcaaaaaa
     2881 taagacgaga atgcgttttg tttacaaaca gtctcacctt ttctgaaatt taagcaagta
     2941 tgtaatagct tttctatttg atccatagta agattgaatg aatatagcca gtttacaaga
     3001 tctagtgaat gcttttctac tatttttgca tcatatatgg gcgtgtattc tttatacccg
     3061 tgattttcca ttatttcact aatagtaata aaatcagata atctagttgc aatcaatttt
     3121 aaaaaatctc tttcggagtt tgtaaatttt agtttttcat tttccgatcc ttcatagtaa
     3181 acatttatat tcccattttc ttttttattt attattaatg gaaaagtcaa tatatcatct
     3241 ggatctaaaa catcatcaat attacttcca taatatgcat ctttatatac tatgcttaca
     3301 ttacatctat caggatatga aaaattaaca cacaaattat ttataatata agtaaaaatt
     3361 tcttcaattt ttttattttt attaaaatta tcatctaaac tatataaaaa tgttaagaat
     3421 ttattttcta tagatttacc tcctatttct tgatttttca taataatatt tttgcaaata
     3481 tacaaataat ttattgatat aaatcaattt ttattgattt attaaacaaa aaaaggaaga
     3541 gcttaaaagc ccttcctttt acactatttt taaaaaaaat atatattatt ctattatgat
     3601 cttttgagta ttcaagtatt tgccatcagc ttgtaaagat atcatatata atccagctgg
     3661 caaattatta acattaatag gtactaaggt actacctact ttcacatcta tagattttgc
     3721 ataaacttgt tgacccaaaa tatttgaaat agtaataaca gcattagtgc taatgggaga
     3781 tactatattt acatttacat aatcactagc aggattagga tatacagtta ctgaatttat
     3841 tgtattatat tcatgaatat catagctagt cactccaata tctgatttaa gaactttcat
     3901 atagataatc tcattttccc attgtgtatg agattcatct tgtagagcat tgccaggacc
     3961 accatcagct tgaaaaatca aatgtatgta atcatctgta tttttagcca tagcgccata
     4021 tacacattct attaaatcat aatcatcacc accagttaat tcagaaaatt cactccaatc
     4081 atcaccacca ttagtagatt tagtagccca aatatgacga taaaatgcgt aactgaattg
     4141 attttcattc aaatgagcaa atgtaacaaa aatattatta tcgtcatcaa tagttatttg
     4201 tggttgagaa actaatccat gattaccata agtaggtact tgctcccatg caccagaagt
     4261 ccaattatca aatataattc cactattatc taaatccatt aaccatccaa taataccgat
     4321 ttcagtagtt tccatatcaa gatcatagat agttcccata tcttcattcc aataagcgag
     4381 ataagaagta aatgggaacc aagaaaattg ttctgtacta ggctcttcaa ccattattct
     4441 tactacacca aatgtagcgt gtagcttacc actattatcc atttctaaag ctacgagccc
     4501 atcacataca taattagtat ctaatggtac cccagtatca aaagtaaatg ggctaggatg
     4561 ttcaaaaact acagtttttg tccaagtatc accaccatca gtagatttca taataaatac
     4621 atcatgccac atattaccca ctataaaagc aagcgtacca tttttaggtt ttgcccaagc
     4681 gtatatatcg gctgaatagg ataattgttc cgcatcagca tccatacctg gtaacacact
     4741 atgtgtccaa ttattaccac catcagtaga gcgagaataa acgatagctg ttcctacacc
     4801 ttgatatggg ttatcaccac catgagcaga taacacatga atagtatcac ctactgtgac
     4861 tactctaggc caagtcatct ctaaaccagt aggagcagca atgtaagttt gtgaccaatt
     4921 tccagctcct tttgcctctc ttttattcat aactaaatca gtagctccag aatgtgatat
     4981 aactatttca ccgtttttaa aaggataata gctgccccag ccagttctaa cagtttctat
     5041 tctagatatc tgcgttcccc atgctgtacc atcataataa ttatatgctg tacctctgtc
     5101 ggctaaagta cctgtacctc tgatccatgt agcgcctatt gtgccatcag gatatagata
     5161 aattctgcta tcaatagatt tgttagactg aatatcatat tcagtattac caataggttg
     5221 attatgaaat atcttttcat tcataggtgc ataagggata ttaacttgag gcactggttc
     5281 atcgccttta aattgtttat tgtattgtgc tgttactgca taatttttta aatatgcagg
     5341 cacttgaggt cttccttgag caaataagct cattgaaaaa cctgctacaa tcattaaaac
     5401 taataaactt ttctttttca taatttttat tttttggggt tttgttacta caaaattaat
     5461 aaaatatttt taataaacaa tagtttaaac agaatatttt ttttaaaaat aaatttatta
     5521 tacaaatata cataaaaata acaatctata aacaaatatt tatagtgagt ctactgaact
     5581 attttttaat aaatttcttt gcagtattag aagtactatt agatatactt attatatata
     5641 taccagctga caaatcagat aaatcgatag tgcaatgatc tttaccttca attactgtag
     5701 aataaacctc agctccaaga atgtttagta tttttataaa atttgcacct ttatcaatca
     5761 aattaatatt taattgatca gatactggat taggataaat atagatgtta gcagaattag
     5821 tataattatt aatacctgct ggtccaataa taacatcgtt taagaaataa taatctattc
     5881 tatcatctga agtgtaattg ccacctaaaa acccaactga tagggggact ttaatattac
     5941 cattacccaa atcaaaacat tgatcagcta aataaaacca ggtagcttga tcttcgacat
     6001 aagtatttcg agttacatta tgtgtttgag aataagtatt attgtctgca tcataaactt
     6061 taataaatat attaggtaac atattatcaa attcattatc tgttgtagtt acccaagtag
     6121 tatcactatc agcccaagca aaaacaatat atttaccatc tggagtacga ctcatctgaa
     6181 aacgatcatc gatataatat ttactaccac cttcttcgta aaacaaaaaa tcatcacacc
     6241 acactgtatc taatacagca gcatcccaac cactagaagt ttggaaaata tcaaatacta
     6301 tccctttcat ataatatcta ttagcataat acgatagtga atcaggatgg tcactccaag
     6361 ctccatgaat atagcaagcg aaatgcaaat gcccattttt gtctatcata gcttctaatg
     6421 gataaccgaa aaatggacgt ccaactcgtg aataataatc agcatgatct tctattgtgc
     6481 ttattcctcc ccaattaaaa gtaattttgt cccaagtatt accaccatca cttgtttcat
     6541 aaataattgg ctgatatgat agtggatcgt aagcgtctgg attacgacca ctgtatacta
     6601 aataaccatt tgtgccatca tcagccattg ctaaagcagc ggtagaatat ccttttggaa
     6661 gtccatcagt aatagcgaaa tctggtacta catttaccct tttccatatt acagagtcac
     6721 cacttgtatc ccattcgcct atattgacta ctgttttaaa ggatgtataa taagtaccat
     6781 catcagtatg agcatcacct aacacaaaaa atttatttcc acgtgattgc atgaataatc
     6841 tattaaataa agacggatta ctggtagtag ggtagatgtt atcatcataa ggaagtattt
     6901 gattattcgt acccgtacca ttaaaaaatt cagatccata gaaatctcct ccccaactgc
     6961 ctcctataag cataggtcca gctacaatga tggcagcatt ttcataatct gaattattat
     7021 taggattata aataactcca ctaggatatc tagccaaata gttgcccgta ctaataggta
     7081 aactccatgg tatcaaagta gtactatcaa aggatgaacc accatcagta gaaaatgatg
     7141 ttctgagcac accactgcct ccatcataag aaggatcttg tctataagta aaagcaatta
     7201 agttaaggtc ctgattagca cttagacaag tttgttctgg tagtataaca gaaaagatat
     7261 ttatagaatg acccatatgt gaaggggaaa ttgctctttt attatgtttt tgtgataatt
     7321 ggtcaagaat agttgcaatt tcattcttgt aagaggcaaa tgatacttgt ccattcggat
     7381 tttgtaaagc tttattgatc gcatttggtt ctactttttt aggcgctata ttaacacctt
     7441 tattctgagc cattaataag ctcacgctaa ctactaatac taatactaaa aatgattttt
     7501 ttcttttcat aatttttatt ttttgaggtt tgttactaca aaattaataa atatatctta
     7561 aattaatcct attttaatct atttttttag ttaaaatcaa atttattgta caaatataat
     7621 taatatttac aattaacaaa tatttatagt gagtctagca aactattttt ttagcaaaaa
     7681 ataattataa aaagagtcaa gtttaaatta tacatatttt attttaataa ttttttaggt
     7741 tctataacgt ttactgtggg taataacatt ttagattgtt ataaataggt tattttttta
     7801 caacaaaggg ctatttgtag cctttttaat tccatctttc tctgcgttct ttgtgcattc
     7861 tttgagaact ttgtggttga tatttaactg tctaataata ataaaactaa taaatgttta
     7921 agtttttcaa gtttata
//
LOCUS       DAOPRN010000075         7922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_7617_length_7922_cov_109.466633, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000075 DAOPRN010000000
VERSION     DAOPRN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7617_length_7922_cov_109.466633"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKI65_07450"
     CDS             complement(<1..298)
                     /locus_tag="PKI65_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78306.1"
                     /translation="MKTLAKKPWITKITEYPDKKLCWFLFVIAILLYLPTITFDYTLD
                     DALVMTGNKYTQQGIKGTKDIFTHDLFEGFFGEGKQIVAGGRYRPFTQFIFAIQK"
     gene            complement(264..1469)
                     /locus_tag="PKI65_07455"
     CDS             complement(264..1469)
                     /locus_tag="PKI65_07455"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOB78307.1"
                     /translation="MKLLRKYKVIFILITINFKLNCQYIPGIEQTDLYFDKLKSKNIG
                     ILCNNNSQLRDKHLIDSLIIYGTKIKKIFAPEHGFYINQPDGEKIFNDKYKTIKKNYI
                     PIISLYGDKYKPTPFDLEGIDIMLIDLQDVGVRCFTYISTVYYMLDACAQKQIPVILL
                     DRANPNGHFIDGPVLDMKFASFVGVVPIPLVYGMTLGELVMMINGEHWLPSRDTCMLE
                     VIKCKNYTHDSLIQLNFSPSPNLPNMASVYAYPHLVLFEGTNMSVGRGTDKPFLLLGS
                     PDFCIEDTIFIPVSIPGKAIKPPFENMQCKGINLTNFSLHFLKYYRKIYMDLLLETYK
                     CYNDKEKFFKLPFFDRLAGTDILRNQIVQGASINNIRASWKIDLESFMQIRQKYLLYE
                     DFSKETMDY"
     gene            1810..3105
                     /gene="eno"
                     /locus_tag="PKI65_07460"
     CDS             1810..3105
                     /gene="eno"
                     /locus_tag="PKI65_07460"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOB78308.1"
                     /translation="MGNIIQLYARQILDSRGNPTIEVEVITDVGEYGKACVPSGASTG
                     KYEAVELRDGEKKYYLGKSVLKAVNNVNTEITSALKGIDITEQRLIDKTLIELDGTPN
                     KSRLGANAILGVSMACAAAGANSTNQTLYRYIGGVNAHILPIPMMNTINGGVHADNNL
                     DVQEFMIMPIKAKTFSESLRMGVEIFHNLKSILKNNGYVTSVGDEGGFAPSLSDNEEA
                     LKLLMEAITKAGYKPGIDVVLALDVAASELYDPKEKVYKLKKSTGKKMTTDEIIDLYK
                     KWVDTYPIVSIEDGLAEDDWDGWQLLTSELGSKIQLVGDDIFVTNTELLKKGIEKKVA
                     NSILIKLNQIGTVTETLDTINFAKRNGYTSIVSHRSGETEDTFISDLAVATNSGLMKS
                     GSPSRTDRIAKYNQLLRIEEYLNNVGDFLGTNFKYYRFK"
     gene            complement(3249..3884)
                     /gene="nth"
                     /locus_tag="PKI65_07465"
     CDS             complement(3249..3884)
                     /gene="nth"
                     /locus_tag="PKI65_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOB78309.1"
                     /translation="MTKKEKLIEILKILETQNPSPTTELKYDNEYQLTVAVVLSAQCT
                     DKRVNLITKNFFKKFKDFKSLSLASIDEIYDLIKSCSYPNNKAKYLKLLSEIVVSKYN
                     GKLPDNYNELITLPGVGPKTANVLLNVLYNAPVIPVDTHVKRVTTRLGLANNKDSPLK
                     VEKKLKAITPDEYLNKLHHWFILHGRYICTAKAPKCEICEIAMFCDFFLTK"
     gene            complement(3892..4857)
                     /gene="nusB"
                     /locus_tag="PKI65_07470"
     CDS             complement(3892..4857)
                     /gene="nusB"
                     /locus_tag="PKI65_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOB78310.1"
                     /translation="MIGRRVLRIKVLQHVYAFHLSGNDDLVSARNNLIKSIADLSNIF
                     YINLYFFFRFIKFYEERIEENKKKLIPSEEDLKPNLVLIDNFYINSLLSNKKLNIEIS
                     DLSPDFAFENDMLWDIYKKMKDWYNYKSFIKKRRYILEDYQKFYSKFFKQFIAFHPLL
                     KSYLESGDLSWVDDQNTMALVTYMWLKNYDPSDINSAEAPKPFKPIKNIGDEDDKAFV
                     VTLFDNTILNKKKWDDEIQKHAKNWDLHRLNILDRYIIHQAMTEIVYCPTIPIKTTID
                     EYIEIATKFCTEKSHIFINGLLQAIIDDYSENGLIKKSGRGLIDN"
     gene            complement(4965..5693)
                     /locus_tag="PKI65_07475"
     CDS             complement(4965..5693)
                     /locus_tag="PKI65_07475"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78311.1"
                     /translation="MASIFSKQKKEKTTSLHLKIDLHNHILPGIDDGAQDIDESLEML
                     EAYQAQGFTKIIATPHVNSERFLNSTDDILEATEIFLEAKEKENIPIQIAVSAEYYAD
                     KHLFYLLEKNDVLSIANKYLLCEFSFYFPPIDAKLIANEIKNAGYIPILAHPERYEYW
                     FNDIKAFEKLKEYGFLFQSNLPSASGLLGPIPKHNFYLLANKGWIDFLGTDAHNAFSI
                     YLMQNIIELNIVQKTLPRILNYYL"
     gene            complement(6078..7640)
                     /locus_tag="PKI65_07480"
     CDS             complement(6078..7640)
                     /locus_tag="PKI65_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOB78312.1"
                     /translation="MKRSNFFIWLGITLSLGIILGWLLNNKQPKLSLNNNGKIYEIIR
                     YLENNYVDSVSFKQLEDLSIENLLSALDPHSTYLTKEDLIVNADPVLGQFEGIGIQFR
                     MEKDTAIVVYVIPNGPSFKKGIMPGDKIIEVNGKSIAGQKLKDVEIVKMLKGPKGTKV
                     TVGIKREGYKDILYYELIRDVIPLITVDAAFLISPNTGYIRINSFSTKTAQEFRTELS
                     NLKKDHKIKNLILDLRDNGGGVLESAVEVANVFLPKNSLIVYLEGKHRPRKDFYAWGD
                     TLGNGLEIAVLVNQFTASASEIVAGALQDNDRAIIIGRRTFGKGLVQEQITLKDSTAL
                     RITVARYYTPSGRCIQRPYSEGYEKYFLDYYESILDTTSLYSLKQNIDTTKYYTVKGR
                     VVHGNGGIIPDTIVIKDYDVPILTFSKTIGSKTFFDNLSYYIQHNRKKLLNLYPDEDY
                     FAKHFKVTDNMMKNLLGDSYLFIGTPHRETFSKIIASYIARELYSPAAYLKVSSSIDQ
                     CILTALKYFNSI"
BASE COUNT         2824 a         1095 c         1050 g         2953 t
ORIGIN      
        1 ctttttgaat agcaaagata aattgagtaa atggacgata gcgaccgcca gctactattt
       61 gtttcccttc accaaagaaa ccttcaaata aatcatgagt aaaaatgtcc tttgtacctt
      121 ttattccttg ttgggtatat ttatttccag tcattacgag tgcatcatcc aatgtataat
      181 caaaggtgat tgtgggtaaa tataacaata tggcgattac aaataaaaac cagcacaatt
      241 ttttatcagg atattctgtt attttagtaa tccatggttt ctttgctaaa gtcttcatat
      301 aataaatatt tttgacgaat ttgcataaaa ctttctagat ctattttcca cgaagctcta
      361 atattattta tagatgctcc ttgaactatc tgatttctta atatatcagt accagctaat
      421 ctatcaaaaa aaggtaattt aaaaaatttt tctttatcat tatagcactt ataagtttct
      481 aataataaat ccatataaat ttttctataa tattttaaaa aatgtaaaga aaaattagta
      541 aggtttatgc ctttacattg catgttttca aaaggtggct tgatagcttt acctggaata
      601 gatacaggta taaaaatagt atcttcaatg caaaaatctg gtgagccaag aagcaaaaaa
      661 ggtttgtctg tacctctacc tacactcata ttagtccctt cgaataatac taaatgagga
      721 taagcataaa cagaagccat atttggtaaa ttaggtgaag gagaaaaatt aagttgaatc
      781 aatgaatcat gagtataatt tttacatttt atcacttcga gcatacaggt atcacgcgat
      841 ggcaaccaat gttcaccatt tatcatcata actaactccc ctaatgtcat tccataaaca
      901 agtggtatcg gtacaacacc aacaaaagaa gcaaatttca tatctagcac tggtccatca
      961 ataaaatgtc catttggatt tgctctatct aacaatatta ctggtatttg cttttgtgca
     1021 catgcatcta acatataata aacagtactt atgtatgtaa aacacctaac ccctacatct
     1081 tgtaaatcta ttaacattat atctatacct tctaaatcaa atggagtagg cttatattta
     1141 tctccatata atgatattat cggtatatag ttttttttga ttgttttgta tttatcattg
     1201 aatattttct caccatctgg ctgattaatg taaaagccat gttctggtgc aaatattttt
     1261 ttaatcttag ttccatatat tattagagaa tctattaaat gtttatctct taattgactg
     1321 ttattattac ataaaatacc aatattttta ctttttaatt tatcaaaata caaatcagtt
     1381 tgttctatac ctggaatata ttgacaattt aatttaaaat ttatagttat taatataaaa
     1441 atgactttat attttcttaa taacttcata tcacaaaaaa tatttcaaaa ataataattt
     1501 tttgaaaatc aagaaaatat tttactaaaa taaaatttaa gttaatttaa ttaatgcagg
     1561 actaaattta taataaaatt aattttgaaa taaagctaat taccttaatt aactatacta
     1621 atatatacat aaatcctaat attaaagaag tttagtaaat tattatgatc atattactat
     1681 ttactcaact taaaatcaat attttatata ataaaaacaa aaaatttatt ttaggtagtt
     1741 ttttattcac ggattcagga attattttaa aaaaattatt taactttgca attcaaaatt
     1801 tttaataata tgggaaatat tattcaattg tatgcacgtc agattttaga ttctagaggt
     1861 aatcctacta tagaagtaga ggtaataaca gatgtagggg agtatggcaa agcttgtgtg
     1921 ccatctggtg catctacagg caaatatgaa gctgtagaat tgagagatgg cgaaaaaaaa
     1981 tattatttag gtaaaagtgt attaaaagct gtaaataatg taaatacaga gataacttct
     2041 gcccttaaag gcattgatat tacagaacaa agattgatag acaaaacttt aatagagctc
     2101 gatggcactc caaacaaaag ccgattagga gctaatgcta ttttaggtgt ttcgatggca
     2161 tgtgctgcag ctggtgctaa tagtactaat caaacactct atagatatat aggaggagta
     2221 aatgctcata ttttacctat accaatgatg aatactatta atggaggtgt acatgcagat
     2281 aacaatcttg atgtgcagga gtttatgata atgcctatta aagctaagac ttttagtgaa
     2341 tctctacgaa tgggagtaga aatttttcat aatttgaaat caatattaaa aaataatggt
     2401 tatgttactt ctgtgggcga tgaaggtggt tttgctccca gtctatcaga taatgaggaa
     2461 gcattgaaat tattaatgga agctattaca aaagctggtt ataaaccagg catagatgta
     2521 gttttagctc tcgatgtagc tgcttctgaa ctttatgatc caaaagaaaa agtctataaa
     2581 ctaaaaaaat caactggaaa aaaaatgaca acagatgaaa taatagattt atataaaaaa
     2641 tgggtggata cttacccaat agttagcatt gaagatggat tggctgaaga tgactgggac
     2701 ggatggcaat tattaactag tgagcttggt tctaaaattc aacttgtagg tgatgatatt
     2761 tttgtaacta acactgaatt gcttaaaaaa ggtattgaaa aaaaagttgc taattctatc
     2821 ctgattaaac taaatcaaat cggtacagta actgaaacgc tcgatactat caattttgct
     2881 aaaaggaatg gatacacttc gatagttagt catcgtagtg gagagactga agatactttt
     2941 atctctgatt tagctgtcgc taccaattcg ggacttatga aatctggcag tccatctcgt
     3001 acggatagga tagctaaata taatcaactt ctaagaatag aagaatatct gaataatgta
     3061 ggagattttt tgggtactaa ttttaaatat tatcgtttta aataatcaag atatttaatg
     3121 atttttgaaa tataggtaaa tttaatcttt ttattttact taatttgaaa atgctttaag
     3181 aatattttat taattgataa ttatcaatta atttgttgat taaatttaat taaaaaattg
     3241 ataatcattt attttgttaa aaaaaaatca caaaacatcg caatctcaca tatctcacat
     3301 ttaggagctt tggcagtgca aatatatcta ccatgtagaa taaaccaatg atgtagttta
     3361 ttaaggtatt catcaggagt tatggctttc aatttttttt ctacttttaa tggtgaatct
     3421 ttgttattag ccaaacctaa cctcgtagtt acacgtttca catgagtatc tactggtata
     3481 acaggggcat tatataaaac atttaataat acatttgcag ttttgggacc tacaccaggt
     3541 aaagttatta actcattata attatcagga agtttaccat tatatttaga aactactatt
     3601 tcagacaaaa gctttaaata tttagccttg ttattaggat aagaacaaga ttttattaaa
     3661 tcataaattt catcgatact tgctagagat aacgatttaa aatctttaaa ttttttaaaa
     3721 aaattcttag ttatcagatt gacacgttta tctgtacatt gagcactcaa tactactgct
     3781 actgtcagct gatattcatt gtcgtatttt aattctgttg taggactcgg attttgtgtt
     3841 tctaatattt ttaatatttc tattagtttt tctttttttg tcataaaaat attaattatc
     3901 tatcaagcct cttccagatt ttttaattaa accattttca ctataatcat ctattatagc
     3961 ttgcaataat ccatttatga aaatatgact tttttcggta caaaatttag tggctatttc
     4021 tatatattca tctatagtag ttttgatagg tatagtagga caatatacta tctcagtcat
     4081 tgcctgatgt attatatatc tatcaagtat atttagacga tgaagatccc aattttttgc
     4141 atgtttttgt atctcgtcat cccatttttt cttatttaaa atagtattgt caaaaagtgt
     4201 tactacaaaa gctttatcat cttcgtcacc aatattttta ataggtttaa aaggttttgg
     4261 tgcttcagct gaattaatat cactgggatc ataatttttt aaccacatgt aagtaactaa
     4321 agccatagtg ttttgatcat ctacccagct aagatcgcca gattccaaat aagacttcaa
     4381 taaaggatga aaagcaataa attgcttaaa aaatttagaa taaaattttt gataatcttc
     4441 taaaatatat ctgcgctttt ttataaaaga tttataatta taccagtctt tcattttttt
     4501 ataaatatcc cataacatat cattttcaaa agcaaaatct ggtgataaat cagagatttc
     4561 aatattcaat tttttattac taagcaagct attaatataa aaattgtcaa ttaaaaccaa
     4621 gtttggttta agatcttcct ctgatggtat taattttttc ttattctctt ctattctctc
     4681 ttcataaaat tttataaatc ggaaaaagaa atataaattt atgtaaaata tatttgatag
     4741 atctgcaata ctttttatta aattatttct ggcagatact aaatcatcat taccagacaa
     4801 atgaaatgca taaacgtgct gcaatacttt gatcctcaaa acccttctac caatcataat
     4861 ataaaattgc aaggcaaaga tataaaaaaa ttgtgttttg aataagaaaa atcattgata
     4921 attttaaaaa attctttttc ctgatatata ttgtattaat ttttttatag ataataattc
     4981 aatattctag gtaatgtttt ttgtactata tttaactcta taatattttg cattaagtaa
     5041 atactaaaag cattatgtgc atcagtgcct agaaaatcta tccagccttt attagctaaa
     5101 agataaaaat tatgcttagg tataggacct aacaaccctg atgcagatgg caaatttgat
     5161 tggaataaaa atccatattc ctttagtttt tcaaatgcct ttatatcatt aaaccaatat
     5221 tcatatcttt cgggatgtgc tagtataggt atatagcctg catttttaat ttcatttgct
     5281 attaatttag catcaatagg agggaaataa aaactaaatt cacataataa atatttattt
     5341 gctattgaca aaacatcatt tttttctaaa agataaaaaa gatgtttatc tgcataatat
     5401 tcagcagaga cagctatttg gataggaata ttttcttttt cttttgcttc taaaaatatc
     5461 tctgtagctt ctaatatatc atcagtacta ttcagaaaac gttcactatt gacatgaggc
     5521 gtagctatga tttttgtaaa tccttgagct tgatacgctt ctaacatttc tagactttca
     5581 tcaatatctt gtgctccatc atctattcct ggtaaaatat gattatgcaa atctattttt
     5641 aagtgcaagc tagttgtttt ttcttttttt tgctttgaaa agattgatgc cattatatta
     5701 attatgtttt ttttgctttg ctaaaataag gattattttt atatttattt aaaaaaaact
     5761 ttctttactt gtcaaaaaga gtatagattt tttatttaat tttgtatact tattaaaaaa
     5821 ttaattaatt ttcaatttta tcatactaaa acttttaata ttatgattaa aattatgcta
     5881 taattttttt ttacaataag gtttatttta tttattattt tagacgttta cagcgaaaaa
     5941 tatttatacc ttataccaaa tgtaccacaa aaacataaaa aatataatga atgataatat
     6001 ttattattaa gttaaaaatc aagatttaga taaacattat gataatttca ttgaagtaat
     6061 tttctaataa tattatttca gatagaatta aaatatttta aagcagttaa aatacattga
     6121 tctatcgagc tactaacctt taagtatgca gcgggcgaat aaagttctct tgcaatatag
     6181 cttgcaatga ttttagaaaa cgtttctcta tgtggagttc caatgaatag gtagctatca
     6241 cctaataaat ttttcatcat attgtcagta actttgaaat gtttagcaaa ataatcttca
     6301 tctggataaa gatttaaaag tttttttcta ttatgttgta tataatagga taaattatca
     6361 aaaaatgttt tagatccaat agttttagaa aaagtcaaaa taggcacatc ataatctttg
     6421 atgacaatag tatcaggaat aatgcctcca ttaccatgta ccactcttcc ttttacagta
     6481 taatatttag tagtgtcaat attttgctta agtgaatata aagaagtagt atctaagata
     6541 gattcataat aatctaagaa atatttttcg tatccttcac tatatggacg ttgaatgcat
     6601 ctgccagaag gtgtataata tctcgctacg gtaattctaa gtgctgtaga atctttcaaa
     6661 gtgatttgtt cttgtactaa tcctttgcca aaagtacgcc tacctattat aatagcccta
     6721 tcattgtctt gtagagcacc tgcaacgatt tcagaagctg aagcagtaaa ttgattaacg
     6781 agcactgcta tttctaatcc gttgccaaga gtatcccccc atgcgtagaa gtcttttcgt
     6841 gggcgatgtt tgccttctaa atatactatt aaagaatttt ttggcaaaaa aacattagct
     6901 acttctactg cactttctaa tactcctcca ccattgtcac gtaaatctaa aatcaggttt
     6961 tttattttat gatccttttt taaattagat aattctgttc taaattcttg ggctgtttta
     7021 gtcgaaaagc tattaatgcg aatataacca gtattaggtg atattaaaaa agctgcatct
     7081 acagttataa gaggtataac atcccttatt aattcataat ataagatgtc tttgtatcct
     7141 tcacgcttta tccctactgt cactttagtt cctttaggcc cctttaacat ttttactatt
     7201 tctacatctt ttagcttttg cccggctatg gatttaccat ttacttctat tatcttgtct
     7261 cctggcatta ttcctttttt gaatgaaggc ccattaggta ttacataaac tactatagct
     7321 gtatcttttt ccattctaaa ttgtatacca attccctcaa attgacctaa aactggatct
     7381 gcattcacta ttaaatcctc cttggttaaa taagtagaat gtggatctaa tgcagataat
     7441 aaattttcaa tagacagatc ttctaattgt ttgaaactca cagaatccac ataattattt
     7501 tctaaatacc taatgatttc ataaatttta ccattattgt ttaaacttaa ttttggttgc
     7561 ttattattta ataaccaccc aagaataata ccaagagaaa gagttattcc caaccaaata
     7621 aaaaaattag aacgtttcat cttttttaca aaaataaata tttttataaa attaaataca
     7681 aagataatat ttgaattagt gaaataaata tatctaaaac atctaatttt tattggatgt
     7741 tattataaga aacagagtat taactaattt ttttagtaat taaacatcta ctttaattgt
     7801 aaaaatttct agagtatgtg ataaggaaat attaattttt tggaggtgta aagggaagga
     7861 ggatttgatg tatatctgtt aaaatttata caggattaat atcagaaaat ttattaaatt
     7921 tg
//
LOCUS       DAOPRN010000076         7838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_7719_length_7838_cov_115.289863, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000076 DAOPRN010000000
VERSION     DAOPRN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7719_length_7838_cov_115.289863"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(550..2205)
                     /locus_tag="PKI65_07485"
     CDS             complement(550..2205)
                     /locus_tag="PKI65_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOB78313.1"
                     /translation="MKKIKNLFIAIILIVIGFISYRAVIGDISFELVKNLDIFTSVMK
                     ELNKNYVDEIQPGKLTKTAIDEMLRSLDPYTEFIPESAMEDYKLMMAGQYGGVGALIQ
                     KLDSFVVISEPYEGFPAYNAGLRAGDIILEVNGISVKGKSTQEVSEMLKGSPDTEVEI
                     TYKNLFDDKINKVKLIRKEIKLPEVPYYGIVAPHIGYISLSSFTINCSNTVKDALLKL
                     KEGDSHLQGLILDLRNNGGGLLNEAVNIVNIFVPKNMEVVRTKGRLPEANRVYKTMQA
                     PIDLEIPLCVLINQNSASASEIVAGALQDLDRAVVIGEQSYGKGLVQNIVSLPYNSKL
                     KITIAKYYIPSGRCIQKIDYGDKSNGVGREISQTEQKIFYTKNKRPVTDAGGILPDIN
                     IDIDSISSFTAAVYIGQYIFKFATLYSKNHPTIASPDKFTITDADYNEFIEYMKDVEI
                     KYSSPLSMELDKLIKLAEEEKKLDLIKNDIELLKEKITLNNEAELRHYKDEIISLLAP
                     EIVARYYFQKGRMQYVLKDDKCTHKAIEILSDNKRYKEILGFK"
     gene            complement(2264..2491)
                     /gene="yidD"
                     /locus_tag="PKI65_07490"
                     /pseudo
     CDS             complement(2264..2491)
                     /gene="yidD"
                     /locus_tag="PKI65_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
     gene            complement(2442..2849)
                     /gene="rnpA"
                     /locus_tag="PKI65_07495"
     CDS             complement(2442..2849)
                     /gene="rnpA"
                     /locus_tag="PKI65_07495"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOB78314.1"
                     /translation="MPNKLKKLRINSKKQIERLFKQPFLLEYPFKVHYLIEDGRGEMH
                     CLIAVSHKQYKKAVDRNRIKRVVRAVMFELMDDINLILKDKNKDLYIALIYIGDKKIN
                     YKLIKEKIIVFLQRLLNYENNKENTCNATIDND"
     gene            complement(3003..3830)
                     /locus_tag="PKI65_07500"
     CDS             complement(3003..3830)
                     /locus_tag="PKI65_07500"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOB78315.1"
                     /translation="MDSNYKILISQNKPNDYEKGPYRALELKHNVIFDFIKLFKVECV
                     NTRDFRNQKIDVNDYFGIIFTSKIALDYFFSLLEQSRINISSEMRYFCLTEQIALYLQ
                     KYMRYKKRKFFFGTGTPDSVVEHIKQFPNEKFILVSSVDGNESFLNALNQSNINFDNL
                     FVCKNVPVDISDIDIREYKMLAFFSPHGINSLFQNFPDYEQRDQAIAVCGEYTKKMAE
                     ERNLKISVYAPTKEFPSLVNALDDFFLKSVNKIDNNKQITMKLSIDEANISQNKNSK"
     gene            5216..7336
                     /locus_tag="PKI65_07505"
     CDS             5216..7336
                     /locus_tag="PKI65_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78316.1"
                     /translation="MNKKNIYYCFFILFIFLSNLVYSQHNWLKDIIFRIDTNVVSYKK
                     DKILYQNKEVIPLRYRVNQEIVEISILLQNNKNIKEIKVGSSKFYNIIESPEKKESDI
                     ISYTIQFIDATIANFVKHKIFVITYDNDTTILEISLQPISDQQIQIFPSQDELYVGEE
                     VTWELITNIPENILTSNIWTEKETYRYRIIEQGGNKYLQLIPTQIGKQQFMIEVPLYK
                     PIIENKNFKYTYTFTTPSFNVKPGRLAYLNITPSEFVLQDILTDNIYEAQIDNNRLLK
                     LNKTYRIEDREDKGVLIAELYTQKAISGNKVLCDVYVYNLHSIDKGVLYIKDGDEAKF
                     ITNFVVLPKTQFQSISISYDGKNWSTTPNVKPGDNVIVRIEGQSLHKSNLIFKGLDIK
                     NIDTIFISSQTIEYRLYIPYNIYIPRIEIVNYNNPTGLYLNIVEHQRPRDYDFLSIDF
                     GEGFINLEDIMAPIFYNKSIKNIVLKGNPNKIDEGEDLYGKQYLEIEVRIVGSRGEVI
                     ELIPWQDFTICPDTNSIRYQHYNKDDCSALEMSFNEILYKKTYSLDPWSRIIITIRHK
                     KEKYTYQIPQKVVEIICERRFGFDIEVSFPAGLLTIMPNSSQISNLNGVSMAMIAQFS
                     FYQKQRYAKLLPIKVGAGFIALNAFNFSSTNIHRDMSLVALISLYPTTKDRKLNFPLY
                     MGGGYFLSENKFFFLVGPGIQVSF"
     gene            complement(7414..7596)
                     /locus_tag="PKI65_07510"
     CDS             complement(7414..7596)
                     /locus_tag="PKI65_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78317.1"
                     /translation="MNSYKNINDKELSIMVYSSLMKKYKINDSQLKEILISISYNNKS
                     DILDSVYDDIYKSSFE"
BASE COUNT         2913 a         1071 c          922 g         2932 t
ORIGIN      
        1 gctatctgat gtggtttgta atcacacata aatacttgac aagtgagata gaatcagaca
       61 ttggtcggct tttaaacctt gcatcctcgg acagatgggc gtgggatgag tattggcggc
      121 tggtgaatac aaagtataga taaactactt caagtaaaac aatgatttgt ttctagaccc
      181 tcttcgattc ccttttgatt gcacttataa tattttagtt aattaaaata ttatcaaact
      241 ttactacatt tttttgggtt ctatcaaatt aaataggatc ttgatttagt gcataagatg
      301 ataggtattt atttgataga tataaaaaaa tatttatcta acaataatat ctaaaaaata
      361 cttaaacttt ttatctatcc ccatttatcc ctgggtctca ttgtcctaat aattaaatat
      421 tacttaataa tttaaataaa tacagtttta agatgtaaaa ataattattt tatgcaaaaa
      481 aaaaatttac ctctccatca cttgctaact tcttacctcc taatcccagg cgcgctactt
      541 taagtaagat tacttaaacc ctaaaatctc tttataccgt ttattatcag acaaaatctc
      601 tatagcttta tgagtacatt tatcatcttt caatacatat tgcatacgtc ctttttgaaa
      661 ataatatcta gctactatct ctggtgccaa taaagaaatg atttcatctt tataatgtct
      721 taattcagct tcattattta gagttatttt ttcctttagc aattctatat cattttttat
      781 caagtctaat tttttttctt cctcggctaa ttttatcaat ttatctagct ccatacttag
      841 tggcgaagag tattttattt ctacatcttt catatattct atgaactcat tataatcagc
      901 atcagtaatt gtaaatttat ctggtgatgc tatagtagga tgattttttg aatataaagt
      961 agcaaatttg aatatatact gaccaatata tactgctgct gtgaaagaag agatactatc
     1021 gatatctata ttaatgtcag gtaatatacc accagcatct gttacagggc gtttattttt
     1081 ggtataaaat attttttgtt ccgtctgaga tatttctcta cccacaccat tgcttttatc
     1141 tccataatct attttttgta tacatctacc agaaggtata taatatttgg caatcgtaat
     1201 ttttaatttt gaattatagg gtaaagaaac aatattttgc actagacctt ttccataaga
     1261 ttgttctcca attactacag ctctgtctag atcttgtaat gcacctgcta ctatttcact
     1321 agcagaagca ctattttgat taattaaaac acataatggt atttctaaat ctattggtgc
     1381 ttgcatagtt ttatatactc tattagcttc tggtaaacgt cccttggtcc taacaacctc
     1441 catgttttta ggtacaaaaa tatttactat atttacagct tcattgagta agccaccgcc
     1501 gttatttcgt aaatcaagta tcaaaccttg taaatgagaa tcaccctctt ttaattttaa
     1561 taaagcatct tttactgtat tgctacaatt aatagtaaat gaagataaag aaatataccc
     1621 aatatgtgga gctactatac cataatatgg tacttcaggc aatttaattt cttttcttat
     1681 taatttaact ttatttattt tatcgtcaaa aagatttttg tatgttattt ctacctcagt
     1741 atctggtgaa ccttttagca tttcgctaac ttcttgagtt gattttcctt ttacactgat
     1801 accattcact tctaaaatta tatcaccagc tctcaatcca gcattatatg ctggaaatcc
     1861 ctcataaggt tcagatatta ctacgaaact atctaatttt tgtattaatg cacctacacc
     1921 accatattga cctgccatca ttagtttata atcttccatt gcactctcag gtataaattc
     1981 agtatatgga tctaagcttc ttagcatttc atctatagct gtttttgtca atttacctgg
     2041 ctgtatctcg tctacataat ttttatttaa ctctttcatt accgaggtga aaatatctag
     2101 attttttact aattcgaaac ttatgtcacc tataacagct ctgtacgaaa taaaaccaat
     2161 aacgattaag attatcgcta taaataaatt tttaatcttt ttcataaata ctaatatttt
     2221 ctaaataatt tgatgattta aatttaacaa atataaattt atcaggtact ggatcataac
     2281 cgtggcctcc ccatggatta catctgagta ccctatatac tgataataaa actcctttta
     2341 ctaatccatg tttttttaat gcttctatag cataagctga gcatgtagga taaaatctac
     2401 atgacttagg aaataaaggc gaaatacact tttgataaag tttaatcatt atcaatagtg
     2461 gcgttacaag tattttcttt attattttca taatttaata aacgttgtaa aaatacaatt
     2521 attttttctt ttattaattt ataatttatt tttttatctc ctatatatat caatgcaatg
     2581 tataaatctt tgtttttgtc ctttaaaatt agatttatgt cgtccatcaa ttcaaacatc
     2641 accgctcgca ctacacgttt gatgcgattt ctatctacag cttttttata ttgtttatga
     2701 cttactgcta tcaagcaatg catctcacct cgaccatctt ctattaagta atgtacttta
     2761 aaaggatatt ctaataaaaa tggttgttta aaaagccttt ctatctgttt tttagaattt
     2821 atacgaagtt tttttagctt attaggcatt atatttaatt taattgatat gtatatatca
     2881 ttataattct taatattgct tattattttt tgtttataac atttatgaaa atatctatac
     2941 tttatctaac atttttgaaa tagcaatatt ttgcattttt tcaaaaaata tttttattct
     3001 gattattttg aatttttatt ttgagatata tttgcttcat caatacttag cttcattgtt
     3061 atttgcttat tattatcaat tttatttaca gattttaaaa aaaaatcatc caatgcatta
     3121 acaagtgaag gaaattcttt tgtaggagca tatacagaaa tttttaagtt tctctcttca
     3181 gccatttttt tagtatattc tccacaaaca gctatagctt gatctcgctg ctcataatca
     3241 ggaaaatttt gaaataaaga atttatgcca tgaggcgaaa agaatgcaag cattttatat
     3301 tctctgatat ctatatctga aatatctact ggcacatttt tgcatacaaa aagattatca
     3361 aaattaatat tactttgatt taatgcattt aaaaaacttt cattaccatc tacactcgaa
     3421 accaaaataa atttttcgtt cggaaactgc tttatatgct ctactacgct atcaggggta
     3481 cctgtaccaa agaaaaattt gcgttttttg tatctcatat atttctgtag atataaagct
     3541 atctgctctg tgagacaaaa atatcgcatc tcactcgata tattgattcg cgattgttct
     3601 aataaactaa aaaaataatc taaagctatt ttacttgtaa aaattatacc aaaataatca
     3661 tttacatcta ttttttgatt tctaaaatct ctcgtattta cacattctac tttaaataat
     3721 ttaataaaat caaatattac attgtgtttg agttctaatg ccctataagg acctttttca
     3781 taatcatttg gcttgttctg agatattaat atcttataat tgctatccat aatatttata
     3841 aatttttata attaactaca cttaatacac ataaattaat attttacatc taaaatttca
     3901 aaacatctat atttatcccg ttaataggta catcagttaa ttgattacta tacttgaaaa
     3961 aaataaaatc acttaaaaaa aagtaaaaac taatagtaat taatatttta tcataaatta
     4021 tttttcgaaa aacttgggta atttgaagtt tattgttgtt tagaatttta tcaatcatta
     4081 tttcttattt taatataata tgttttttaa ataactatac ccaattacaa aaggaactaa
     4141 ttcttgaatg caaaaataca aaaaatatat gatatgtaaa ttgtttttaa attttaaatt
     4201 atgatttatc attcttatcc atctatatat aaatatcata taaataaaaa taacgcacat
     4261 cgttaaaata acctcttttt gatatgaagt gtatattaat aagaatataa acattataaa
     4321 cattatatgc aaatatgaac ccagataata aagcaaatct gcttttaatt gttcttttat
     4381 ttcgaaaacc catgaataaa ttgacataaa aaaaatctta tagaatacaa tagccaaaat
     4441 gagaattgca aggtttgaaa ataaaaattt atcaaaaaaa gtatatgtaa taaaatttaa
     4501 aaaaatataa acagtaatta aataaataaa aactaataaa taattaaaaa tgttattaag
     4561 agaaaatttt ttaaaaaaaa tttttctttt agaatcaatg taaaagctag acaaaatatt
     4621 tttacaatat gggaaattag attttattat gataaggaat aatattaata ttatggcaaa
     4681 tattataagt acgcattgtg ttaaaggtaa tttatagcta acaggcatat gcgaaatata
     4741 tgcttgatga tttattagtt ctattatgct atcattacat aaatctctat ttaaatgaaa
     4801 atacacatta aaaaaactac tatctatagt atcactccat tgaatataag aataaggaat
     4861 atcactcata ggcattgatc aaagcacaaa attaataatt attaagatac tattgagaaa
     4921 ttatattatt ctaaacttta gtaaaatact acaaaaatct aaattatttt gaatgatcaa
     4981 attttaaaat tatcatgaaa gaatattgaa ttactattga agtgacttgt ctcgatacaa
     5041 agaaacattt ttattaaatt tgaataaaat tttattttaa aaatttgtat gcttggtttt
     5101 taatttatat ttttgtaatt aataaatgga aagtattgat aataaatcat aatatttgta
     5161 tacaaatatt ttattatatt tgataaaatt agacttaatt ttttacaatt gatttatgaa
     5221 taaaaaaaat atatattatt gtttttttat tttatttatt tttttgtcca atttggtata
     5281 ttctcaacat aattggctaa aagatattat atttagaata gatactaatg tagtttctta
     5341 taagaaagac aagattttat atcaaaataa agaagtaata ccactgcgat atagagtaaa
     5401 tcaagaaata gtagaaatat caatattatt gcaaaataat aaaaatatta aagaaataaa
     5461 ggtaggtagc tcaaagtttt ataatatcat agaatctcct gaaaaaaaag aatcagatat
     5521 cataagttat actattcaat ttatcgatgc taccattgcc aactttgtaa aacataaaat
     5581 atttgttatc acatatgata atgatactac aatattagag ataagtctac aacccataag
     5641 tgatcaacaa atacaaatat ttcccagtca agatgaatta tatgtgggtg aagaggttac
     5701 atgggaattg ataacaaata taccagaaaa tattctaact tctaatatat ggactgaaaa
     5761 ggaaacatat agatatagaa taatagagca aggtgggaat aaatatttac aacttatacc
     5821 cacacagata ggtaagcagc aatttatgat agaagtaccc ttatacaaac ctattataga
     5881 aaataaaaat ttcaaatata catatacatt tactacaccc tcatttaatg tcaaaccagg
     5941 tagattggca tacttaaata tcactccctc agagtttgta ttgcaagata tccttacaga
     6001 caatatctat gaagctcaaa tagataataa tagattacta aaattaaata aaacataccg
     6061 aatagaagat cgtgaagata aaggtgtttt aatagctgag ctatatactc aaaaagctat
     6121 atctggtaac aaagtattat gtgatgtata tgtttataat cttcattcta ttgacaaagg
     6181 agtattgtat attaaagacg gagacgaagc aaaatttata actaattttg tagttcttcc
     6241 aaaaacacaa ttccaatcta taagcatatc ttatgatggt aaaaattggt caactacacc
     6301 taatgtaaaa cctggtgata atgtaatagt aagaatagaa ggtcaaagct tacataaatc
     6361 aaatctgatt tttaaagggt tggacattaa aaatattgat accatcttta tttcttcaca
     6421 gaccatcgag tacagactgt atattcctta taatatttat attccgcgta tagaaatagt
     6481 caattataat aatcctacag gattgtatct aaatatagta gaacatcaac gtcctagaga
     6541 ttatgatttt cttagtatag attttggcga aggcttcatt aatctagaag atataatggc
     6601 gccaatattt tataataaat ctatcaaaaa tatagtatta aaaggaaacc caaataaaat
     6661 agatgaaggc gaagatttgt atggtaaaca atatttagaa atagaggtca gaatagtagg
     6721 atcacgtggc gaagtaatag aactcatacc atggcaagat tttactatat gtcctgatac
     6781 taattcgatc agatatcaac attataataa agatgattgc tcagcattgg agatgtcttt
     6841 taatgaaata ttgtataaaa aaacttattc tctagatcca tggtctcgta taataataac
     6901 tataaggcac aaaaaagaaa aatatactta tcaaatacct caaaaggtag tagaaataat
     6961 atgtgaacgt agatttggtt tcgatataga agtctcattt cccgctggat tattaactat
     7021 aatgccaaat tcaagtcaaa tttctaatct taacggagtg agtatggcga tgatagctca
     7081 atttagtttt tatcaaaagc aacgatatgc aaaattattg ccaataaaag taggtgctgg
     7141 cttcatcgca ttaaatgcat ttaatttttc tagtacaaat atccatagag atatgagctt
     7201 ggtagcactt attagcttat atcctactac aaaagatcgc aaactaaatt ttccattata
     7261 catgggcggc ggttattttt tatcagaaaa taaatttttc tttttagtag gccctggcat
     7321 tcaagttagc ttttaaaata tagaataatc ttatattatt tttttattaa ttaataaatg
     7381 taaaaaatat aaatgatcta aagctaaatt tatttattca aatgatgatt tatatatatc
     7441 atcataaaca ctatctaaaa tatcagattt attattatat gatatagaaa tcaaaatttc
     7501 ttttaattgt gaatcattta tcttgtattt tttcattagt gaactataaa ccattataga
     7561 taattcctta tcattaatat ttttgtaaga attcaaattt ctcaattgaa aaataagata
     7621 ttctgctgta tataaatcct ttaaaatagg tgtaatagat tcattattat aagtattggt
     7681 atcaaatttt gaatggttat cttttttttg gcaattcgta aaagtcagaa ttaataaaat
     7741 taatgaaaaa aatatatata taaaactttt atacctcata taaaaacaat aagaaaaaag
     7801 tccgcaaaat tgcggacttt taaattattt gtttggtg
//
LOCUS       DAOPRN010000077         7809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_7750_length_7809_cov_95.014444, whole genome shotgun sequence.
ACCESSION   DAOPRN010000077 DAOPRN010000000
VERSION     DAOPRN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7750_length_7809_cov_95.014444"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..634)
                     /locus_tag="PKI65_07515"
     CDS             complement(119..634)
                     /locus_tag="PKI65_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78318.1"
                     /translation="MAQKKNQPTPPSTSSSQVVEQPKKAYGWQAIPNLTDQQIEQIKT
                     LQAEHKKQITILKNQLGEKKAQLQTATSWASYNASNAEKILDEIYAIKLQMAKEKLSF
                     HNAVRNILDDNQKIYWDKKFSMNPSNKMMRQSCQYSKSEMMHNCQAHKYDHHSYDHHS
                     YGPEHKFCDKH"
     gene            complement(528..1169)
                     /locus_tag="PKI65_07520"
     CDS             complement(528..1169)
                     /locus_tag="PKI65_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78319.1"
                     /translation="MFSISIGLNVALLVILIYDHYRISKLEKQNQAIEWRFPRHKAPR
                     PPLIDDLRLSHEQDIDITKEMHLYRTHIKILADSMNIIRDSLFRELNNDQQNDTVINN
                     YIDKLVYYDRKMHEELVKHLKAVKLILDDEQFKIFMENIKNKFKPRCKQTNKKYKSYE
                     KVNEYFNYGHPYCGIAVFDGSEKKSTNTTFNIIEPSSRTTQKSLWLASHTQPN"
     gene            complement(1190..1591)
                     /locus_tag="PKI65_07525"
     CDS             complement(1190..1591)
                     /locus_tag="PKI65_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78320.1"
                     /translation="MKCEEIQKTLIEKSIEELSNTELSHINNCDACKQFAMLLDIEKQ
                     LKDDECWYQPSEYNLKILNERLQSRSWQKVVPKRTLINTIPWIAAIITLILSVYIFLI
                     SQTKNINNNIDETHYYYTDVMFTNYLNNDEQ"
     gene            complement(1666..2229)
                     /locus_tag="PKI65_07530"
     CDS             complement(1666..2229)
                     /locus_tag="PKI65_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOB78321.1"
                     /translation="MKKHNDNIIDKFDILVEKTKQPLFRLALNILKDEEMAMDVVQDT
                     YVKAWENWTSFRGESDPKTWLYRITINLSINQLKKKQRVINFNKFTNENIKDDEKIHD
                     FEPEDPNTIEKYLNCKERKYIIKNAIDDLPINQKLVLQLFYYEGFHYREISEILDISI
                     SAVESLLFRARRNLKLKLANYYKRNEL"
     gene            complement(2503..4026)
                     /locus_tag="PKI65_07535"
     CDS             complement(2503..4026)
                     /locus_tag="PKI65_07535"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOB78322.1"
                     /translation="MEFNERDKKIIIDHGLSIEEVNRQIEILRKGMNYIELIRPATIN
                     NGIIELDDIKKNYYKNKFEKDQYFYTIEKFVPASGAATRMFKALYQYLDKYDNAKPST
                     SGLEWFFNNIQKFAFFDDLQKALSDRGYTYDQAINEKKYDMIVRLVLDNDGLGFSNKP
                     KALLPFHKYDNGYRTSLEEHIIEGLHYAKDNNGVNIHFTISPEHLDMFNELLNKILIH
                     YEKQYKSKINISYSFQKPSTDTVALYKDNLELVRDESGNIIFRPGGHGALIENLNDLS
                     SKIIFIKNIDNVVPDDKKADTIEYKKILGGLLMDVVEQVQAFLTILDDANVSEGELDS
                     ILNYCKNEWQQPIAEYVYSLDKMEKIDYLYSLLNRPIRVAGMVRNVGDPGGGPFIIID
                     DNGEESLQIIESAQVSMNDPEQKNIWISSTHFNPVDIVCYTYNYKGEKFDLLDYIMEN
                     TYFITEKTYKGTEILALEHPGLWNGAMGYWLTIFAEVPLSTFNPVKELKDLLKPAHL"
     gene            complement(4243..4875)
                     /locus_tag="PKI65_07540"
     CDS             complement(4243..4875)
                     /locus_tag="PKI65_07540"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOB78323.1"
                     /translation="MKTKIFQTITIDDIEYIISKIKSYPFIELRLDHILHLDFKFIFS
                     KTNNNEIIATMQINKKNKQRAIDLLIYMIDLGATSVDINIESLNYSEIDNLIQYSKIK
                     NCNTILSIHNFNGIFSEKEIEIFINQKNQKKLNFIKIVVNPTNDKEYNDFKKWYNIFD
                     NVIFLCTGKYSLASRLYAIERCVPFVYSLADGSNPLFDGHIYYSELVKYL"
     gene            complement(4872..5744)
                     /locus_tag="PKI65_07545"
     CDS             complement(4872..5744)
                     /locus_tag="PKI65_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOB78324.1"
                     /translation="MNEIKTDWKGFFTFLAIFLLAVLIFNFFMSYILLITHQINNPNA
                     IMLANTIAHLLSFTGVGIAFPLLQKYNLSKYYQNNPLKSFSFFILTFFVVILIQPSLS
                     LLTKLPGYISLPPNLENYFNQYEGIVNELLKSTLAGGGIGYLLLNIIYIAVVPAIGEE
                     LIFRGIIQRYLINFTKNAWLGIIITGLIFSLFHLSWNTILAIWFFGIILGLVYYTTGN
                     IWYSIFIHFLNNLMAALSYWLASNELIPYTIEEIDSANFPIWAEILSLILMILFIVFL
                     LKNIKNKREISINQ"
     gene            5790..6773
                     /gene="dusB"
                     /locus_tag="PKI65_07550"
     CDS             5790..6773
                     /gene="dusB"
                     /locus_tag="PKI65_07550"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOB78325.1"
                     /translation="MQIGKYELEKFPVILAPMDGITDKIYRKICKEMGASLTYSEFIS
                     ADSIVREAKRSFDKISKNDDEHPFVVQIFGNNPIIMGEAASIVECAGADIIDLNFGCP
                     MKKIVSKGAGAALLKNPQLMIDITKNVVNKVSIPVTVKTRLGWDESDIIIEKLVPRLQ
                     DCGIAALTLHARTRKQLFAGEADWSYFLKLKSNSSIKIPIIGNGDIDSPQKAKDAFYA
                     YNVDGVMIGRAAIGNPWIFKNCYSFLNNDLLLPPPTIDDRLSVCKRHLDELVMLKGER
                     IAIIEMRKFFKSYFKGIPNIKQYRLALLNSTNLNEIYDIFESISRFAINHN"
     gene            6847..6920
                     /locus_tag="PKI65_07555"
     tRNA            6847..6920
                     /locus_tag="PKI65_07555"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6881..6883,aa:His,seq:gtg)
     gene            complement(7164..>7809)
                     /locus_tag="PKI65_07560"
     CDS             complement(7164..>7809)
                     /locus_tag="PKI65_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOB78326.1"
                     /translation="FIQIYYFLFTHSKRYRTNINLKQGIWITIGSVIGAQLGSFLSKF
                     IPDIGLGKAFSVFLLLSALIFIIRGIKDIKYGDDFFDKSANKSNFIIKFLKSNVTLTG
                     LILGFFVGILCGILGAGGGVMILLILVFIMQYPLHIGIGTSNLIMAFTAASGTIGHAL
                     TANLPLKPSLIGSVGTVIGCLFAAKFANRIDEKLLSIIVGTVFAILGVIMIVIL"
BASE COUNT         2732 a         1085 c          999 g         2993 t
ORIGIN      
        1 aaatttaata aattttctga tattaatcct gtataaattt taacagatta aatattagaa
       61 tataaaaagg tcaactgttt ttttaacagt tgaccttttt taagattatt tcatcatctt
      121 aatgtttatc acaaaattta tgttctggac cataactatg atggtcataa ctatgatggt
      181 catatttatg agcttggcaa ttatgcatca tttcactttt actatattgg caagattgtc
      241 tcatcatttt atttgaagga ttcatactaa attttttgtc ccaatatatt ttttgattgt
      301 catctagtat gtttcttaca gcattatgaa atgataattt ttcttttgcc atttgtaatt
      361 ttatagcata aatttcatca agaatctttt ctgcatttga tgcattataa gatgcccacg
      421 aagtagcagt ttgtaattga gctttttttt cgcctaattg attctttaag atagttattt
      481 gctttttatg ctctgcttgc aaagttttaa tttgttctat ttgttgatca gttaggttgg
      541 gtatggcttg ccaaccataa gcttttttgg gttgttctac tacttggctc gatgatgttg
      601 aaggtggtgt tggttgattt tttttctgag ccatcaaaga ctgcgatccc acaataagga
      661 tgcccataat taaaatactc gttaactttt tcatagcttt tatatttttt atttgtttgt
      721 ttacaacgtg gcttaaattt atttttaata ttttccataa aaattttaaa ttgctcatca
      781 tcaagaatta attttacagc ttttaaatgc ttaactagct cttcgtgcat ttttctatca
      841 taatatacta gcttatctat ataattattt atcaccgtat cattttgttg gtcattattt
      901 aattctctaa ataatgaatc tctaataata ttcattgaat cagctaatat tttaatatga
      961 gttctatata agtgcatctc ttttgtaata tctatatctt gctcatgtga caatcgtaaa
     1021 tcatcaatca gaggtggtct aggtgcttta tgcctaggaa acctccattc tatagcttga
     1081 ttttgttttt ctaatttact tattctataa tgatcgtaaa ttaaaataac taatagagct
     1141 acatttaatc ctatggaaat actaaacaat acaattaaag tttttgtttt cattgttcat
     1201 cattatttaa gtaatttgta aacataacat ctgtataata ataatgggtc tcatcaatat
     1261 tattattaat atttttagtt tgagaaataa ggaaaatata aacagataaa ataagagtta
     1321 ttatagctgc tatccaaggt atagtattta tcaaagttct ttttggtaca accttttgcc
     1381 acgaacgtga ttgtaaccgt tcattcaaaa tttttaaatt atactcagag ggctgatacc
     1441 aacattcatc atcttttaat tgtttttcga tatctaatag catagcaaat tgtttacaag
     1501 catcacaatt attaatatgt gataactcag tattcgaaag ctcttcaatt gatttttcaa
     1561 ttaatgtttt ttgtatttcc tcgcatttca tatttattag acattttaaa tttaaaaaac
     1621 ttgtgtttaa ttgttaaaat attattttta taataaaata aacatttata attcatttct
     1681 tttataataa ttagctaatt ttagtttgag attacgcctg gctctgaaaa gtaatgattc
     1741 aactgcagaa atactaatat ctaatatttc tgatatctct cgatagtgaa atccttcata
     1801 ataaaataat tgtaaaacta atttttgatt aataggtaga tcatcaatag cattttttat
     1861 tatatatttt ctttctttac aatttaaata tttttctatt gtatttggat cctctggttc
     1921 aaaatcatgt atcttttcat catcttttat attttcatta gtaaatttgt taaaattaat
     1981 aactctttgc tttttcttta attgatttat tgataaatta atggtgattc tatataacca
     2041 agttttagga tctgactcac ctctaaagct tgtccaattt tcccaggctt taacgtaagt
     2101 atcttgcact acgtccatag ccatctcctc atcttttaat atattgagag ctaaacgaaa
     2161 taatggttgc ttagttttct ctactaagat atcaaattta tcaataatgt tatcattatg
     2221 ttttttcata attttgatat gatgagaagt gagatgatat tggtaattat tttttgcaaa
     2281 ttaaagaatt tttttctaga tctccagttt tttactgata atctacaact aatgatattg
     2341 agtcgtttaa acatttaaac cttttgcctt gtttcttatc acttttgcga tctctcactc
     2401 tatataatcc atttttttaa aatatttagt gatttttaat ttatgtttta gtttagcaag
     2461 attatatacg ataaaagcaa atacattatt tgatgagttg gtttataaat gtgcaggttt
     2521 tagtagatct ttaagttctt ttacaggatt aaaagtagat aaaggcactt ctgcaaatat
     2581 ggttaaccaa taacccatgg ctccattcca taaacctgga tgctccaatg ctaatatttc
     2641 tgtaccttta tatgtttttt ctgttatgaa atatgtattt tccattatgt aatcaagcaa
     2701 atcaaatttt tcacctttgt aattgtaggt atagcagaca atatctacgg gattgaaatg
     2761 tgtagaacta atccatatat ttttttgttc tggatcattc atacttactt gtgcagattc
     2821 tattatttgc aaagattctt cgccattatc atctataatg ataaaaggac ctcctcctgg
     2881 atcacctaca tttcttacca tacctgctac tctgataggt ctattaagca aagaataaag
     2941 gtagtcaatt ttctccattt tatctaaact atacacatat tcagcaatag gttgctgcca
     3001 ttcatttttg caatagttta aaatactatc caattctcct tctgatacat tagcatcatc
     3061 gagtatagtt aaaaatgcct gcacctgctc tactacatcc attagtaatc cacctagtat
     3121 ttttttgtat tctatggtat cagccttttt atcatcaggc actacattat ctatattttt
     3181 tataaatata attttacttg ataaatcatt taggttttct atcaatgccc cgtggccccc
     3241 aggcctgaaa ataatattac cactttcatc tctcaccaat tctaaattat ctttatatag
     3301 tgctactgtg tctgtactgg gcttttggaa actatatgaa atatttattt tgctcttata
     3361 ttgtttttca taatgtatta aaattttatt taaaagctca ttaaacatat ctaagtgttc
     3421 gggacttatg gtaaaatgaa tatttacacc attattatct ttagcataat gcaatccttc
     3481 tattatatgt tcttctaacg aagtacgata tccattatca tatttatgaa agggtaatag
     3541 agctttaggt ttattgctaa aacctaatcc atcattatca aggactagac gaactatcat
     3601 atcatatttt ttctcattta ttgcttggtc ataagtatac cctctatcag ataatgcctt
     3661 ttgcaaatca tcgaaaaaag caaatttttg aatattatta aaaaaccatt ccaagccaga
     3721 agtactgggt tttgcattat catatttatc taaatattga tataatgctt taaacatgcg
     3781 agtagccgca ccagaagctg gtacaaactt ttcaatcgta taaaaatatt gatccttttc
     3841 aaatttgttt ttataataat tttttttaat gtcatcaagt tcgattatac cattattgat
     3901 agttgcaggt ctgataagct ctatataatt cattcctttt cgcaaaattt ctatttgtct
     3961 atttacctct tctattgata atccgtgatc aataatgatt tttttatccc tttcgttaaa
     4021 ttccataatg ttatttttta caaatttcat ttaatttttg aaattaagca aaatctttta
     4081 attaattacc taaattataa ataaattata atgtcaaagg aataaggaaa ttggctgggt
     4141 tgtatgaaga tgagacaagt gtagtattag tggtttaaaa agttattatt tgtgattttt
     4201 tttcaaaaaa atttatccta tttattaata acaaattgat ttttacaaat atttcactaa
     4261 ttcactataa taaatatgcc cgtcaaataa aggattacta ccatcagcaa gcgaatatac
     4321 aaaaggtaca catcgctcaa tagcatatag tctagatgct agactatatt taccagtaca
     4381 taagaaaata acattatcaa atatattata ccatttttta aaatcattat attctttatc
     4441 atttgtaggg tttacaacta tttttataaa atttaatttt ttttggtttt tttggttaat
     4501 aaaaatttct atttcttttt cagaaaatat accgttaaaa ttgtgaatag ataatattgt
     4561 attgcaattt ttaatcttag aatattgaat taaattatct atttctgaat aattaagact
     4621 ttctatgttt atatctacag atgttgctcc aaggtcgatc atgtaaataa gtaaatctat
     4681 agctcgttgc ttattttttt tatttatctg catagtagct attatctcat tattattagt
     4741 tttagaaaaa ataaatttaa aatctaaatg caatatatga tctaatctaa gttcaataaa
     4801 aggataagat tttatttttg aaattatata ttctatatca tctatagtaa tagtctgaaa
     4861 aattttagtt ttcattgatt tatgctaatt tctcttttat ttttaatatt tttcaataaa
     4921 aaaactatga ataaaatcat taaaatcaaa gaaagtatct cagcccatat aggaaaattg
     4981 gcagaatcaa tttcttctat agtgtatggt atcaattcat tgcttgcgag ccagtaagat
     5041 aatgcagcca ttaaattatt taaaaaatgt ataaatatag aataccaaat attacctgta
     5101 gtataataaa ctaaaccaag tataatacca aaaaaccaaa tagccaaaat agtattccat
     5161 gacaaatgaa acaagctaaa tatcaatcct gtaataatta tacctaacca agcattttta
     5221 gtaaaattta ttagatatct ctgtattata cctctgaaaa taagctcttc acctatagca
     5281 ggtaccactg ctatatagat tatatttaat aataaataac caataccacc gcctgccaaa
     5341 gtacttttca ataattcatt aacaatccct tcatattgat taaaataatt ttctaaatta
     5401 ggcggtaaac ttatataacc aggtaattta gttaataaag aaagtgaagg ttgaatcaat
     5461 atgactacaa aaaaagttaa tataaaaaac gaaaaacttt ttaaaggatt gttttgataa
     5521 tatttcgata aattatattt ttgtaataaa ggaaaagcaa tacccacacc agtaaatgat
     5581 aataaatgag ctatagtatt tgctaacatt atagcattag gattatttat ttgatgagta
     5641 atcaaaagaa tgtaagacat gaagaaatta aatatcaata ctgctaatag aaaaatagct
     5701 aaaaatgtaa agaagccttt ccaatcagtt tttatttcat tcatttttat taattttact
     5761 atgtataaat gcaaaattac tcaaaaacta tgcaaatagg aaaatatgaa cttgaaaaat
     5821 ttccagtgat actagcccct atggatggaa taacagataa aatctataga aaaatatgca
     5881 aagagatggg ggcgagcctt acgtattctg aatttatttc tgctgattcc atagttagag
     5941 aggcaaaaag aagttttgac aaaatcagta aaaatgatga tgagcatcca tttgtagttc
     6001 aaatatttgg taataatcca ataataatgg gagaagctgc atcgatagta gaatgtgccg
     6061 gagcagatat tatagatttg aattttggct gtccaatgaa aaaaatagtg tctaaaggtg
     6121 ctggtgctgc tttattaaaa aatccacaat taatgataga tattacaaaa aatgtggtaa
     6181 ataaggtttc tattcctgta actgtaaaaa caagattggg ctgggatgag tctgatatta
     6241 tcattgaaaa gcttgtacct agattgcaag attgcggtat agctgcattg accttgcatg
     6301 cacgtacgag aaaacaactt tttgctggtg aagctgattg gtcttatttt ttgaaattga
     6361 aatctaattc tagtattaaa ataccgataa taggtaatgg agatattgat tcgccgcaaa
     6421 aagcaaaaga tgccttttat gcttataatg tagatggcgt tatgatagga agagcagcta
     6481 ttggtaatcc ttggatattc aaaaattgtt atagtttttt aaataatgat cttttattgc
     6541 caccacctac catagatgat agattgagtg tatgtaaaag acacctcgat gaattagtaa
     6601 tgctcaaagg ggaacgtata gctattatag aaatgcgaaa attttttaaa tcttatttta
     6661 aaggcatacc taatattaaa caatataggt tagcactatt aaattctaca aatctaaatg
     6721 agatatatga tatttttgaa agtatatcga gatttgctat taatcataat taatagttat
     6781 tataaaaata aatttaaaaa aaatagaatc tatttcataa tttattatta attttgcaaa
     6841 aatatggtgg atgtagctca acaggtaaga gcaccagatt gtggatctgg gggttgtggg
     6901 ttcaagtccc atcatccacc cttaaagttt tatttcttat aaacttatat tacattaaac
     6961 tatttaaata gatgatgata gtgaaagtgg tatatgtagg tgaaataaga gatttagatg
     7021 tttataactt tactaattta taatttctta taatttaaaa ataataaatt tacataattt
     7081 tgaaattgaa ataaataaaa ttataagtta ttagttgaat ttaagcatct attatcttac
     7141 attttcaact aatgacatat tttttataaa atcactatca ttattacacc gagaatagca
     7201 aagacagtac ctactattat gctaagtaat ttctcatcaa tcctattagc aaatttagca
     7261 gcaaacaagc atccaataac tgtaccaaca gaacctatta gagatggttt taatggtaaa
     7321 ttagcagtaa gagcatgtcc aatagtgcca gatgcagctg taaatgccat aatcaaatta
     7381 gatgtaccta tacctatatg taatggatat tgcattataa aaactagaat taataaaatc
     7441 atgaccccac ctccagcacc taaaatacca cataaaatac caacaaaaaa gcctagtatt
     7501 aatcctgtta atgtaacatt agatttcaaa aatttaataa taaaattact tttatttgca
     7561 cttttatcaa aaaaatcatc accatattta atatctttaa tccctctaat tataaatatc
     7621 aatgctgata ataataaaaa cacactaaat gctttgccta acccaatatc agggataaat
     7681 ttagagagaa aactacctaa ttgtgctcca attactgaac ctattgttat ccatatacct
     7741 tgtttaaggt ttatattggt tctataacgt tttgagtggg taaataaaaa atagtaaatt
     7801 tggataaaa
//
LOCUS       DAOPRN010000078         7762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_7810_length_7762_cov_203.967432, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000078 DAOPRN010000000
VERSION     DAOPRN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7810_length_7762_cov_203.967432"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(281..1291)
                     /locus_tag="PKI65_07565"
     CDS             complement(281..1291)
                     /locus_tag="PKI65_07565"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78327.1"
                     /translation="MKKIVENTSKYLAMSKEENKKFLDHLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFEKFEGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVRRNVIKHKEVVTNHRQKRGIYSLSTVKKKASEFTNWIYFKFR
                     GVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNITYVNLN
                     IY"
     gene            1737..2480
                     /locus_tag="PKI65_07570"
     CDS             1737..2480
                     /locus_tag="PKI65_07570"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOB78328.1"
                     /translation="MKSKILKAIIYSLAIIIAAALLGNAYKSRVKTPNTITVKGKADK
                     EIHSDIARWNLMFNVTHPNLKEAYKKTQANTDIVFNYLKSNGLDDNEIRISGIDINKT
                     YETVRIPLGNNDYEYKNQFTGYRLKRNFFIETNKLDTINILVSEISNLIAQDIEITSQ
                     TPEYYYSKLDSVKHELIEKASEDAIERAKKIAKSNNQKIGKIKSAYIAPFQVTGLYSN
                     EDYTWGGVLNTSDIIKICTVTVTMEVEIK"
     gene            complement(2976..4010)
                     /locus_tag="PKI65_07575"
     CDS             complement(2976..4010)
                     /locus_tag="PKI65_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOB78329.1"
                     /translation="MAKSINNTIPKSDIPLTPKDFESDQIIRWCPGCGDHMIVNSLKK
                     VFAEIGYRKENYVVISGIGCSSRLPYYVNTYGFHGIHGRANAIATGAKIANPKLSIWV
                     ATGDGDSLAIGGNHFIHVVRRNLDMNIILFNNQIYGLTKGQYSPTSPLGTKTTTSPYG
                     TIEHPFNTGELVLGAQGTFFARSLDILVDLTTDILFEAAKHDGTSIVEVFQNCVIFND
                     GAYSEITNKEKRDDNVLIVKHGEPMIFGKNNNKGLILNKFNLEVVTIGENGVTEDDIL
                     VHDAKNPSPFIHLMLAKMHHPWVFGVIRAVPQPTYDDLMEEQISMVKAKSNIKCMDDL
                     LNSGDTWEVK"
     gene            complement(4024..5868)
                     /locus_tag="PKI65_07580"
     CDS             complement(4024..5868)
                     /locus_tag="PKI65_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOB78330.1"
                     /translation="MNEENKQMSDRKQVDDIVIKFVGDSGDGIQLAGNIFADTLAHMN
                     LDLATFPDFPAEVRAPRNTTFGVSGFQIHFSNKKVLTPGDEYDVLVCFNPASLKVNLK
                     NVKEGGIIILDSANFTPRSLQTAGYTSNPLEDNSLEKYNVIKVPVSEIILNINSDLDK
                     RTAEKTKNMFFLGLLYNMFDFDLKHAINYINNKFKSKSQIIEANLNSLEAGYRYSENI
                     ENFPKFYLSKCELLPGKYRDIQGNIATAWGLMAAAEKAGLQLFVGSYPITPATDILSE
                     LAKHRDLGVKTFQAEDEIAGITSAIGASFAGALAVTTTSGPGLSLKVEALGLAVMTEL
                     PLVIVDVQRAGPSTGMPTKSEQTDLYLAMFGRNGEAPIPIISATSPIDCFYAAFEAAK
                     IALEHMTPVILLSEGYIGLGSGIFRIPKMTELPKINPPFADPNNKYYPYQRDEEWYVR
                     KWAIPGTPGLQHRIGGLEKSDIYGAVTTDPKNHEKMVINREEKVKRIARKWPLQRITG
                     PENPDLLVVSWGGTKGQVEGAVQNLSTKGYSISHTHFRYINPLPLNTHDILKSGKKVI
                     VCELNRGQFANILKMNYQDITFYQYNKVQGQPFTIKELENAFLNLLNS"
     gene            complement(6188..7186)
                     /locus_tag="PKI65_07585"
     CDS             complement(6188..7186)
                     /locus_tag="PKI65_07585"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78331.1"
                     /translation="MKTIVNDLEGYLSMGKQEHLLFLDQLYILMNKINSDAVDYQTLK
                     AKSTPPLCPECDSRHIVKNGTQNNQQVYLCKECGRSFRDSTGTFVYKIHKREKMLDYI
                     RCMLEGKTLRACAAEVGISLPTSFAWRHRIITALRSFEDNINFFGVVESDELLMQYSE
                     KGRKYKNIKERIEANKKKKKKVAILATQDRSGNMLFKQFGLDKVKRVEVEQLLKDKVS
                     GNSVLCIGNNVEFRKLRTNDLKKQQIIVNNDRQDGLYSLSIIQKHKSIFKYWINNKFR
                     GVATKYLQSYIMWYVIAHKYLLKQTIADIGRMLNLASSDRRAWYKYKYLREIKAIF"
BASE COUNT         2635 a         1209 c         1095 g         2823 t
ORIGIN      
        1 tgttaatatt agtttattaa ttatagtgta gttaaatatc aaccacaaag ttctctaaga
       61 atgcacaaag aacacagaga aggatgaaat taaaaggaca ataaatggca ctttgtgagc
      121 tttgtgtaat aacttggtgt tctctgtggt aaaataaaca acctaaataa ttaaagatag
      181 ttatttttat gtaacaatct aaaattttat tacccaaatt ataacctaag atatgttgga
      241 tgtggagaat ttggaacttg aaaactaatt agtcggaaaa ctaatatatg tttaaattaa
      301 catatgttat attcactaac tctctatacc gataccacgc ccatctgtcc gaagatgcga
      361 ggttgagcat tcgcccagta ttgcttacag tcttgcctgt gaggtattta cccatcacta
      421 cataccacat aatgtagttt tgcaggtatt tggtagctac gcccctaaat ttgaagtata
      481 tccaattcgt gaattcacta gcctttttct tgacagtact taggctatat atacctcgct
      541 tttgtctatg gttagttact acttctttat gcttgattac attccttcta acctttttga
      601 atgctttttt attactgcta cacatgactc catcctttgg catcttagcc ttcagaaatt
      661 gttctatctg ttctgaactt agctttttgc cttcaaattt ttcaaacaac aagttgccag
      721 ctctgtcagt agcagctaat acggctactt tctttggctt ttgcttccta gctcgcttca
      781 gctcttcggc atttttgtat attctgcctt tttcggaata atccatcaat agttcatcca
      841 tctctactac cccgaaaaaa ttgatattat cttcgaaact ttgtaaagct gccaatattt
      901 tgtgtcgcca agcgaagctt gttggtaggc tgatgcctac ttctttagca caagctctga
      961 gacttttgcc tgctaccata catctgatgt agtcgagcat tttctctttc ttttgcaacc
     1021 tgtaaacaaa agtacctgtg gtaactctaa actgtctgcc gcagtctttg cagcgatagt
     1081 tttgcatccc attttgtaaa ccatttttaa taatgtgatt gctacgacag tctgggcata
     1141 gtggtggggc actgagtatt ttcagacctt gataatcgac tacatcggct tctaattttt
     1201 tcattagaga ataaaggtgg tctaagaatt ttttattctc ttccttgctc atagcgaggt
     1261 atttactggt gttttctact atttttttca tgaattaaaa tttttgtaaa aatataaaaa
     1321 ttatttaact tataaacttg aaaaacttaa acatttatta gtttttttat tattagacag
     1381 ttaaatatca accacaaagt tctcaaagaa tgcacaaaga acgtagagaa aaatggaatt
     1441 aaaagggcta taaatggtac tttgtggact ttgtgtaata acttggtgtt ctctgtggta
     1501 aaaaaaatca acctaaataa ttaaagatag ttatttttat gtaacaatct aaaatgtttt
     1561 tacccacaat aaacgttata gaacccgatt ttttaatcaa gtttatatga aatgtgttta
     1621 cagcatttta gattatttta ttctattagt aattattata aaaaatacag gtacatttct
     1681 taatcatatt aaatttatta tatatttttg ttaaatttat taaaaaatat ttcattatga
     1741 aaagtaaaat tctcaaggct ataatttata gtttagcaat tataatagct gcagcattac
     1801 tagggaatgc atataaaagt agagttaaaa cacctaatac tattaccgta aaaggtaaag
     1861 ctgataaaga aatacatagc gatatagctc gatggaatct aatgtttaat gttacgcacc
     1921 ctaatttaaa agaagcttat aaaaaaaccc aagctaatac agatatagtt tttaattact
     1981 taaaaagtaa tggattagat gataatgaaa tacgtatttc gggaatagat atcaataaaa
     2041 cctatgaaac agtgagaata ccattgggaa ataatgatta tgaatataaa aatcaattta
     2101 caggatatag attgaagcgt aattttttca tagaaactaa taaattagat actataaata
     2161 tattagtaag tgaaataagc aatttaatag ctcaagacat agagattact tctcaaactc
     2221 ctgaatatta ttattctaaa ttagatagtg ttaagcatga attaatagaa aaagcatcgg
     2281 aagatgcaat agaaagagca aagaaaattg caaaatcaaa taatcaaaag atagggaaaa
     2341 tcaaatctgc ttatatagca ccatttcaag ttactggttt atattctaat gaagattata
     2401 cttggggtgg ggttttaaat acttctgata ttataaaaat atgtactgtg accgtaacta
     2461 tggaagtaga aataaagtaa taaatagata tatttactta attataatag cttataatag
     2521 ttgtaatata taaacaacgt atatataaaa aatgatgtaa tcaaaaattt tgttctataa
     2581 cgttttgtat ggataaagat taaaaaaaga gtagttttat aaaaaatttg ttaatattag
     2641 tttattaatt atagtgtagt taaatattaa ccacaaagtt ctcaaagaat gcacaaagaa
     2701 cacagagaag gatgaaatta aaaggactat aaatggcact ttgtggactt tgtgtaataa
     2761 cttggtgttc tctgtggtaa aaaaaatcaa cctaaataat taaagatagt tatttttatg
     2821 taacaatcta aaatgttttt acccacaata aacgttatag aaccaaaaat tctgttatct
     2881 aaaataatta attatgcttg tgcataaact tttacatcaa attaaataga atattatata
     2941 taatttttaa gtttttaaaa aaacaaataa aattattatt ttacctccca agtatctccg
     3001 ctattaagta aatcatccat acatttgata tttgatttag cctttaccat actaatttgt
     3061 tcttccatca aatcatcata agtaggttgt ggaacagctc taataacacc aaatacccat
     3121 gggtgatgca ttttagctaa cattagatgt atgaaaggtg aaggattttt agcatcatgt
     3181 actaatatgt catcttctgt tactccattt tcaccaatag ttactacttc tagattaaat
     3241 ttatttagta ttagaccttt attattattt ttaccaaaaa tcattggttc accatgtttt
     3301 actatcaata cattatcatc gcgtttttct ttatttgtta tttcgctata tgcgccgtca
     3361 ttaaatatta cacaattctg aaaaacttct actattgagg tgccatcatg ttttgctgct
     3421 tcgaagagta tatctgttgt taaatctact aaaatatcca gagatctagc aaaaaatgtt
     3481 ccttgtgctc ctagcacgag ttcgccagta ttaaatggat gttcgatagt accatatgga
     3541 gaagttgttg ttttagtacc taatggagat gtaggtgaat attgtccttt agtaagtcca
     3601 taaatttgat tattgaagag gataatattc atatctaaat ttcttcttac tacgtgtatg
     3661 aagtgattcc caccaatagc taatgagtca ccatctccag tagctaccca tatacttagt
     3721 ttaggattag caatttttgc acccgttgct atagcattag ctcgtccatg aataccatga
     3781 aaaccatagg tattaacata ataaggcaat ctagaactgc aacctatacc agatattact
     3841 acgtagtttt cttttctata accaatctcg gcaaatactt tttttaatga attgactatc
     3901 atgtggtccc cacaacctgg gcaccatctt attatctgat cactttcaaa atcttttgga
     3961 gtaagtggta tatcagattt tggtattgta ttatttattg atttagccat aatttaatat
     4021 tttttatgaa tttaataaat ttaaaaatgc attttctaat tctttaatag tgaaaggttg
     4081 cccttgcact ttattgtatt gataaaaagt tatatcttgg taattcattt taaggatatt
     4141 agcaaattgc cctctattaa gttcacagac aataactttt ttacctgatt ttaagatatc
     4201 gtgagtattt agaggtaaag gatttatata acgaaaatga gtgtgagaaa tactataacc
     4261 tttagtagat aaattttgaa cagcgccctc tacctgacct ttagtaccgc cccaactgac
     4321 aaccaatagg tctggatttt caggtcctgt tattcgttgc aatggccatt tacgagcaat
     4381 acgttttacc ttttcttctc tattaatgac cattttttca tgatttttag gatctgttgt
     4441 aactgcacca taaatatctg atttttctaa tccaccaata cgatgctgga gccctggagt
     4501 accaggtata gcccattttc ttacatacca ttcttcatcg cgttgataag gataatattt
     4561 gttatttgga tcagcaaaag gtggatttat tttaggtaat tcagtcattt taggtatacg
     4621 aaaaatacct gaacccaaac ctatataacc ttcagaaagt aatataacag gtgtcatgtg
     4681 ttcaagggca attttagcag cctcaaaagc cgcataaaag caatctattg gcgaagtagc
     4741 agatattatc ggaataggag cctctccatt gcggccaaac atagctaaat ataaatcggt
     4801 ttgctcactt ttagtaggca tcccagtaga tgggcctgca cgttgtacat ctacgataac
     4861 gagtggcaac tctgtcataa ctgctaggcc tagagcttct acttttaatg ataagccagg
     4921 gccagaagta gtagtaactg ctaatgctcc agcaaaactt gctccaatag cagaagtgat
     4981 acctgctatc tcgtcttctg cttgaaatgt tttcactcct aaatctctat gttttgctag
     5041 ttctgataaa atatctgtag caggtgtgat aggatacgaa cccacaaata attgtaatcc
     5101 agctttttca gctgccgcca tcaaacccca tgctgtcgct atattgcctt gaatatctct
     5161 gtattttcct ggtaaaagtt cacattttga taagtaaaat ttaggaaaat tttcaatatt
     5221 ttcactatat ctatatccag cttctaatga atttaaatta gcttctatta tttgtgattt
     5281 agatttaaac ttattattta tataattaat cgcatgcttt agatcaaaat caaacatatt
     5341 gtatagaagt cccagaaaaa acatattttt agttttttca gccgttcttt tatctaaatc
     5401 actattaata tttaaaataa tttctgaaac tggcaccttt attacgttat atttttctaa
     5461 actattatct tctaatggat tagaagtata tccagctgtt tgtaaactgc gaggtgtaaa
     5521 attagcacta tctaatatta taataccacc ttccttaaca tttttaagat ttacttttaa
     5581 tgaagctggg ttaaaacaaa ctaaaacatc atactcatct cctggtgtta atactttttt
     5641 attactaaaa tgtatttgaa atccagatac accaaaagtc gtatttcgag gtgctctaac
     5701 ctcagcagga aaatctggaa atgtagctag gtctaaattc atatgtgcta atgtatcagc
     5761 aaatatatta cccgctaatt gaataccatc accagaatct ccgacaaatt ttataacaat
     5821 gtcatctacc tgctttctat ctgacatttg cttattctct tcattcataa tatctatttt
     5881 ttattttatt gcaaagttag aagttttttt catataatca taataaataa tttaactatt
     5941 ttattaatca tttatatata ccgattatgt tattattaaa ctaaataaat gatctataaa
     6001 actaatttaa aaaatagaaa tagcaatata aagtaatatt aaacaattta tttactgtaa
     6061 taaaattttt tacataatca aaaatataga agctttttat aattattaat aaaggaaaag
     6121 tatgataagg aattaagtta tgaataaatt gttttatgtt ttaagaaata tgtgaaattt
     6181 tagaatgtta aaatatagcc ttgatttctc ttaaatattt atacttatac catgctcgcc
     6241 gatctgaaga tgctaaattg agcatacgtc ctatatctgc aattgtttgt tttaataaat
     6301 acttatgtgc aattacatac cacattatat aactttgtaa atatttggta gctacacctc
     6361 tgaatttatt atttatccaa tatttaaata tgctcttatg cttttgaatg atagataaac
     6421 tatataatcc atcctgtcgg tcattattta caattatctg ttgcttcttt aggtcattag
     6481 ttcttaactt tctgaattct acattattac caatgcataa aacactattt ccagatacct
     6541 tgtcttttag cagttgttcc acttctactc tttttacttt atctaatcca aattgtttaa
     6601 atagcatatt accactccta tcttgggtag ccaatattgc tactttcttc tttttctttt
     6661 tatttgcttc tatacgttct tttatatttt tatattttct acctttctca gaatattgca
     6721 ttagtaattc atctgattct actacgccaa aaaagttgat attatcttca aaacttctca
     6781 atgcagtgat tatcctgtgt ctccaagcga agcttgtagg taagctgata ccaacttcag
     6841 cggcacaagc tctgagagtt ttgccctcga gcatacaacg aatgtaatcg agcatctttt
     6901 ctcttttatg tattttgtac acaaaagtac cagtagaatc tctgaaactt ctaccacact
     6961 ctttgcagag atacacttgt tgattattct gagttccatt ttttacaata tgtcgactat
     7021 cacattcagg acataatgga ggggtgcttt tggcttttag agtttggtaa tctacagcat
     7081 cagaattgat tttattcatc aagatataga gctggtctaa aaacagaagg tgttcttgtt
     7141 tgcccataga aagatatccc tctaaatcat ttacaatggt tttcatagca ttttaatttt
     7201 attaatttat acaaaaataa taaaattatt tatcaatgtt atatttaaac attaattttt
     7261 aagagtatta ttttatcgtc attaaataat atttaactag aatgatgttt atatactttt
     7321 ataaaataat atgtgtgtga ttgttttaat taaaaagttt atggttttgt aacattttgt
     7381 atgggtaaag attaaaaaaa gaatagtttt ataaaaaaat tgttaatatt ggtttattta
     7441 ttattagaca gttatatatc aaccacaaag ttctataaga atgcacaaag aacacagaaa
     7501 aagatgaaat taaaaggcat ataaatggca ctttgtggtt aaaaaataac ctaaataatt
     7561 aaaaaataat gatttttttg taacaatcta aaattttatt acacaaatta taaccttaag
     7621 atatgttgga tgtggagaat ttgaaaacta atcaatttgg aaaactaata tatgtttcaa
     7681 tcaacatatg ttaaattcac tagctcccta taccgatacc acgcccacct gtccgaggat
     7741 gcgaggttga gcattcgccc ag
//
LOCUS       DAOPRN010000079         7640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_7962_length_7640_cov_119.350560, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000079 DAOPRN010000000
VERSION     DAOPRN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7962_length_7640_cov_119.350560"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKI65_07590"
     CDS             complement(<1..223)
                     /locus_tag="PKI65_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78332.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRC"
     gene            complement(338..1414)
                     /gene="mnmA"
                     /locus_tag="PKI65_07595"
     CDS             complement(338..1414)
                     /gene="mnmA"
                     /locus_tag="PKI65_07595"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOB78333.1"
                     /translation="MSNIALLISGGVDSSVAALQLVQQGYKPTLFYIVIGPQEKFDGY
                     NCSIEEDIEMCKWIADKLNLKLEIVNLHQEYWSMVMNYHLESLKKGLTPNPDILCNRL
                     IKFGAFIDKFANSFDIIATGHYANVIEEDNLKWLSTAKDQNKDQTYFLANITYTQLSK
                     AYFPLTYLTKEEVKMIAKRHDFPTSMRKESMGICFIGKNNYDDFIRNKLGNLEGHIID
                     INNNKILGEHDGHWFYTIGQRKGLKLPGGPWFVVKKDIEKNIIYVARGYRPYEVYKDK
                     INFVNFEVVTPGAEQYMKDCMEIYFKIRHSPTFNKGKIYWDNKTNGHVDSLLPIHGVA
                     AGQYIVIYDIDRQKVLGAGVIVVS"
     gene            complement(1407..2165)
                     /locus_tag="PKI65_07600"
     CDS             complement(1407..2165)
                     /locus_tag="PKI65_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOB78334.1"
                     /translation="MEALEVIFKHRSIRKYKKNSIPDDILLNILKAATRAATNGNMQL
                     YSIIITTDEQDKKELFPLHLNQEMVLEAPVLLTFCADLNRFTKWCLYRNANPGYDNLL
                     SLMSAIADTILAAQNAIIAAESYGLGTCILGTTLYTAKEHIKFFNLPKLVLPFISITI
                     GYPDEDPPLTDRLPLEAVIHPGKYQDYDKKTIDIYYQEKENLDIYKDIVKENGLENLA
                     QVFTEKRYTKKDNEEISIKLWEIIKEQGFFQYNE"
     gene            complement(2199..3233)
                     /locus_tag="PKI65_07605"
     CDS             complement(2199..3233)
                     /locus_tag="PKI65_07605"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOB78335.1"
                     /translation="MKKIISILIICIALSTYLFAQKGSYTFGVAFYNIENLFDTINDP
                     NKNDEEFLPDGIKKWTSDKYFKKLDNIAYVIDQLNKNIGGKGVVFIGLSEVENINVLK
                     DLVKREHIAKYRYIPILIEGPDRRGVDVGFLFRPDYFKLIKAEAKNLYIPENPNFKTR
                     NQLVIKGLLFEQDTVFVIVNHWPSRSGGEKRSAPLRAAAAQLCRNTVDSILAVNSNAQ
                     IFIMGDFNDTPTNASILKILKAAPTLTEAKKTNLYNPYYDLQEKKGLGSYAYNDVWSF
                     LDQIIITYNIAACKSNICLKNACVFNKPFLLQTEGNFAGYPLRTFVGNNFMGGYSDHF
                     PVYLLLEYKK"
     gene            complement(3308..4384)
                     /locus_tag="PKI65_07610"
     CDS             complement(3308..4384)
                     /locus_tag="PKI65_07610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB78336.1"
                     /translation="MLKYFIIIIIIVYCISIFYSAIIILHKPKQKFIEKTKITDYSVI
                     IPVKNEATNIVLCIKSILKQSFSPHKIIIVDDHSTDDILEILENNFFNNDLIQYIKLN
                     KNLSGKMAAIKTGLIASKTHWNFITDGDCMWNKNLSENFVISANKRAISFPIVYKNYT
                     NFFSKLLSVELLLLNSVGWASILKNKPFLISGAGMMLIKDDYIEFCDNFDLPVSHGDD
                     MFFLEYLRKHFGPNVVQINLKPSAIIHTLPPNNLKEYLHQRARWASKTPYYKNILSWY
                     WAILTTGVQLLIPLSCLINFNFVIFIVCLKSISEFILYTVSSNTLDLPFYPLACLVFS
                     LFSWILFIFILLQWFIGFDWKRND"
     gene            complement(4560..5549)
                     /locus_tag="PKI65_07615"
     CDS             complement(4560..5549)
                     /locus_tag="PKI65_07615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOB78337.1"
                     /translation="MHVTIVYKGKIPTKYYGGTARDIWYLGKELVKLGNKVTYLVDKG
                     SFCPFADIIYIDKYKNINEQIPKETDIVNLHSEFDEEIEKPFLLSVHGNTNNPNKVFP
                     INTNFVSQNHASRYGAEAFVYNGIDWADYPEPNFSIKRNGYHFLGKAAWRVKNLKAAI
                     KIAKKNKKPLEVMGGKRVNFSMGIRITLNPNIHFHGMVDDIYKAEIMNKSEALLFPVL
                     WHEPMGLAVIESLYYGCPIIATPYGSLPEIVGNEFGFLSNSLSDLIDAATNIEQYNRL
                     HCHQYAYENFNANKMAIKYLMYFEKILNGDNINNNNPRLKVVQVKKFLDFKWK"
     gene            complement(5655..6638)
                     /locus_tag="PKI65_07620"
     CDS             complement(5655..6638)
                     /locus_tag="PKI65_07620"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOB78338.1"
                     /translation="MTKINVSYLGMNLSSPIIVSSCGLTKSIDNLKEFESNGAGAVVL
                     KSLFEEEIRHEVSNLVKFSGEFPYPETEDYIRAYVSDKSTNEYLELIKKAKSSMSIPV
                     IASINCVTSHEWANYAKEIELAGADALELNIFILPTSKNQTAEEIENNYVKIVTSVAK
                     KIKIPIAVKITQHLTNPIHTAYSMELSGAKAVVMFNRMFRPDIDIDNLLVSPGNIFSS
                     PEEIQEIIKWIALTYANVDIDLCATTGIQNGKTAIKVMLAGANVVAISSILYKKGAPV
                     IKEMNETINTWMNEKKFKNTQDFIGKLSYKNIPNPAAFERIQFMKHFSGIE"
     gene            complement(6755..7417)
                     /locus_tag="PKI65_07625"
     CDS             complement(6755..7417)
                     /locus_tag="PKI65_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78339.1"
                     /translation="MIIRKKVHNIIENYSNILIGNSIKVIKNKFIKLFIFINTLLFIS
                     MASIQNCCAQGSNSWELNGNAIYEAEFIGTTNNMPLIFKTNKNEGFRLNSNGNVGIAN
                     TSPVYKLDVCGTIRSTQEIIVEPSDSSCQWPDFVFDSSFQLQLFNERIELIRSQKHLP
                     FIPSSTDIKDNGMPVYETIKGIIQNIEELYLYMEQMERRIEQLEEENHNLKELLPKES
                     LK"
BASE COUNT         2638 a         1158 c          950 g         2894 t
ORIGIN      
        1 tgcagcgata gttttgcatc ccattttgta aaccattttt aataatatga ttgctacgac
       61 agtctgggca tagtggcggg gcactgagta ttttcagacc ttgataatcg actacatcgg
      121 cttctaattt tttcattaga gaataaaggt tgtctaagaa ttctttattc tcttccttac
      181 tcatagcaag gtatttactg gtgttttcca ctattttttt catgaattaa aatttttgca
      241 aaaatataaa aattatttaa catataaact tgaaaaactt aaacatttat taattttcga
      301 ttaacttcca caaaattatt atattcgtaa acttatttta ggacacaact attactcctg
      361 ctcccaaaac tttttgtcta tcaatatcat atatcactat atattgccca gcagctactc
      421 catgtatggg tagcaaagaa tctacatgtc catttgtttt attatcccaa taaatttttc
      481 ctttattaaa agttggagaa tgacgtattt taaaataaat ctccatgcaa tctttcatat
      541 attgttcagc acctggcgta acaacttcga agtttacaaa atttatttta tctttataaa
      601 cttcatatgg tctatagcct cttgctacat agatgatatt tttttcaata tcttttttta
      661 ctacaaacca tggtccacct ggtaatttaa gcccctttcg ttgtcctatt gtataaaacc
      721 aatgcccatc atgctctcct aaaattttat tattatttat atcaataatg tgaccttcta
      781 aattaccaag tttattacga ataaaatcat cataattatt tttgccaata aaacatatac
      841 ccatactttc ttttcgcata gatgtaggaa aatcgtgccg tttagctatc atttttactt
      901 cttcttttgt caaataagtt agagggaaat aagcttttga taattgtgta taagtaatat
      961 tagctaaaaa atatgtttga tctttgtttt gatctttcgc agtagatagc catttcagat
     1021 tatcttcttc tatcacattg gcatagtgtc cagtagctat aatatcaaat gaattagcaa
     1081 atttatcaat aaaagctcca aatttgatta atctattaca aagtatatca ggattgggag
     1141 tcaatccttt ttttaagctt tctaagtgat aattcataac catagaccaa tattcttggt
     1201 gtaggtttac aatttctagt tttaaattta atttatcagc tatccactta cacatctcta
     1261 tatcttcttc tatagagcaa ttatacccat caaacttttc ttgaggccct attactatgt
     1321 aaaaaagtgt aggtttatac ccttgctgta caagttgcaa agcagcaacg gaactatcta
     1381 ctccacctga tattaataat gctatattac tcattatatt gaaaaaatcc ttgctctttt
     1441 attatctccc acagtttaat agatatctcc tcgttatctt ttttagtata tcgtttttct
     1501 gtaaatactt gtgcgagatt ttctaatcca ttctctttaa caatatcttt ataaatatct
     1561 aaattttctt tttcttggta ataaatatct attgtctttt tatcataatc ttgatattta
     1621 ccggggtgta tgacggcttc taaaggcaac ctatctgtta gaggtggatc ttcatctgga
     1681 tatcctatag taatgctgat aaagggcaat actaatttag gtaaattaaa aaatttgatg
     1741 tgttctttag ctgtgtataa agtagtacct aaaatacaag tgccgagacc ataagattcg
     1801 gcagctatta tagcattttg tgctgctaaa atagtatctg ctatagcact catcagagag
     1861 aggaggttat catagccagg attagcattt ctatatagac accatttggt aaatctattc
     1921 aaatcggcac aaaaagttaa taaaactgga gcctctaaaa ccatttcttg atttagatgc
     1981 aaaggaaata gttctttctt atcttgttcg tcagtagtga taataatgct atacaattgc
     2041 atatttccat tagttgctgc tctggtagct gcttttaata tatttaataa tatatcgtcg
     2101 ggtatactat tttttttata ttttctaata ctgcgatgtt taaaaataac ttctaatgct
     2161 tccatggttt tttttaaaaa taaaaatatc caaaaaaatt attttttata ctctaataac
     2221 aaataaacag gaaagtgatc gctatatccg cccataaaat tattacctac aaaagttcta
     2281 agtggataac ctgcaaaatt gccttcagtt tgtaaaagaa aaggtttatt aaaaacgcat
     2341 gcatttttta agcaaatatt agatttacaa gcggctatat tatatgttat tattatttga
     2401 tctaaaaaac tccatacatc attgtatgca taagaaccta atcctttttt ttcttgtaaa
     2461 tcataatatg ggttataaag atttgttttt ttagcttcag ttaaggtagg agcagctttc
     2521 aaaattttca aaatgctagc gttagtagga gtatcattaa agtcgcccat tataaaaatt
     2581 tgtgcattag aattaactgc taaaatagaa tctacagtat ttctgcagag ttgagcagca
     2641 gcagctcgaa gtggcgctga acgcttttca ccaccagaac gagaaggcca gtgattgact
     2701 attacaaaaa ctgtatcctg ttcaaataat aaacctttga ttacaagctg attacgagtt
     2761 ttgaagttgg gattttctgg tatatataaa tttttagctt cagcttttat aagtttaaaa
     2821 tagtcggggc gaaataaaaa acctacatct acacctctac gatctggccc ctctatcaaa
     2881 ataggtatat atctatattt tgctatatgt tctcttttta ctaaatcttt taaaacattt
     2941 atattttcta cttcagacaa ccctataaaa actacacctt tgccaccaat atttttattt
     3001 aactgatcta taacataagc aatattgtct agttttttaa aatatttatc ggaagtccac
     3061 tttttaatgc catctggcaa aaattcctca tcatttttat taggatcatt tattgtatca
     3121 aacaaatttt caatattata aaatgcaaca ccaaaagtat atgacccctt ttgagcaaat
     3181 aaatatgtcg atagtgcaat acaaataatg agtatactta ttattttttt catagttaaa
     3241 taatattata aattaattaa atgcaaaatt agaaaaaaag cttgactttt aataataatt
     3301 ttttttatta atcatttctt ttccagtcaa aacctataaa ccattgcaat aaaataaaaa
     3361 taaataatat ccatgaaaat aaactaaata ctaggcaggc tagaggataa aatggtagat
     3421 caagagtatt agatgagact gtatacaaaa taaattctga tatcgatttt aagcagacaa
     3481 taaaaatgac aaaattaaaa ttgattaaac aacttaaagg aattaataat tgaacacctg
     3541 tggttaagat agcccaatac catgataata tatttttata ataaggggtc ttagacgccc
     3601 atcgcgctct ctgatgcaaa tattctttta aattatttgg tggcaaagta tgaataatgg
     3661 cactgggctt taaatttatt tgtactacat taggcccaaa atgttttctt agatattcta
     3721 aaaaaaacat gtcatcacca tgagatacag gcagatcgaa attatcacaa aactcaatat
     3781 aatcatcttt tattaacatc atacctgccc ctgagataag aaaaggtttg ttttttaaaa
     3841 tgcttgccca tccgacacta ttcagcaaaa gcaattctac acttaagagc ttagaaaaga
     3901 aatttgtata atttttataa acaatgggaa aagaaatggc tctcttattt gctgatatta
     3961 caaaattttc acttaagttt ttattccaca tacaatcacc atctgttata aagttccaat
     4021 gagttttact agctattaac cctgttttga tagctgccat cttgccagat agatttttgt
     4081 ttaactttat atattgaatt agatcattat taaaaaaatt attttctaat atttctaaaa
     4141 tatcatctgt actatgatca tctacgatta ttatcttatg gggtgaaaaa gattgcttta
     4201 atatagattt aatacacaat actatatttg tagcttcatt tttgacagga ataatgactg
     4261 aataatcagt tattttcgtt ttttcaataa atttttgttt aggtttatgt aaaataataa
     4321 tagcactata aaagatacta atgcaataaa caataatgat aataataata aaatacttaa
     4381 gcatgttaaa tttcaaaaag gcaaaaatat aaaacttagt aaaaacacat caaaatatta
     4441 ctattatttg catatactta ataaatattc aaaaacaaat cattttagta aacagatatg
     4501 taagtgttgt tataatactt ttgggtatta ttcatttttt taaattaaaa aaaaactgtt
     4561 tatttccatt taaaatccaa aaatttttta acttgtacta cttttaaacg tgggttatta
     4621 ttattaatat tatctccatt taaaattttt tcaaaataca ttaaatattt aattgccatt
     4681 ttattagcat taaaattttc ataagcatat tggtggcaat gtagtctatt atattgttct
     4741 atattagtag ctgcatctat taaatctgat aaagaatttg ataaaaaacc aaactcatta
     4801 ccaacgattt caggtaaaga accataaggg gtagcaatga taggacatcc ataatacaag
     4861 ctttctatta cagctagtcc cattggttca tgccaaagaa cagggaataa aagagcttca
     4921 gatttattca ttatttcagc cttataaata tcatctacca taccatgaaa atgtatattt
     4981 gggtttagag tgatgcgtat acccattgaa aaattgactc gtttgccccc cattacttcg
     5041 agtggttttt tattcttttt agcaatttta attgcagctt ttagattttt tactctccaa
     5101 gcagccttac ctaaaaaatg atatccatta cgttttatag aaaaatttgg ttcaggataa
     5161 tcggcccaat caatgccatt ataaacaaaa gcctcagctc catatctact agcatgattt
     5221 tgcgagacaa aatttgtatt aataggaaaa actttattag gattattagt atttccgtgt
     5281 acagataata aaaaaggctt ttcgatttcc tcatcgaatt ctgaatgcaa atttactata
     5341 tctgtttctt ttggaatttg ctcattaata tttttatatt tatcaatgta tatgatatct
     5401 gcaaaagggc aaaaagatcc tttatcaact aaataagtaa ctttattgcc taatttcacc
     5461 aattctttac ctaaatacca tatatctctc gccgtaccac cataatattt tgttggtatt
     5521 ttacctttat atactatagt tacatgcatt ttttatcaaa attatgaatt tttattttta
     5581 tctaatcaaa atcatttttt taatgaatat tgttttattg aaaaagaaat aatgattaaa
     5641 tttaataaat gaacttattc tataccagaa aaatgtttca taaattgtat acgttcaaaa
     5701 gcagctggat ttgggatatt tttatatgaa agtttaccaa tgaaatcctg tgtattttta
     5761 aatttttttt cattcatcca agtatttata gtttcattca tttcttttat tacaggagcg
     5821 ccttttttat ataagatact agaaatagct acaacattag ccccagcaag cataacttta
     5881 atagctgttt taccattttg aatgccagta gtagcacaaa gatctatatc gacatttgca
     5941 taagtgagag ctatccattt aataatttct tgaatttcct ctggactact aaatatatta
     6001 ccaggtgata ctagtagatt atcaatgtct atatcaggtc taaacattcg attaaacatg
     6061 actacagctt tagctccaga taattccata gaatacgctg tatgaatagg atttgtcaga
     6121 tgctgcgtga ttttaacagc tattggtatt ttgatttttt tggctacaga agttacgatt
     6181 ttcacataat tattttctat ttcttcagca gtttgatttt ttgaagttgg taaaataaaa
     6241 atatttaact ccaaagcatc agcaccagca agttcaattt cttttgcata atttgcccat
     6301 tcatgactcg taacgcagtt tatactggct attacaggaa tactcatgct tgatttagct
     6361 ttttttatta attctaaata ttcattagta gatttatctg aaacataagc tctaatataa
     6421 tcctcggttt caggataggg aaattcacct gaaaatttta ctaaattgga aacctcatgt
     6481 cttatttctt cttcaaacaa tgattttaaa acgactgcac cagcaccatt agattcaaat
     6541 tcttttaaat tatcaataga tttagttaat ccgcaactcg aaactataat aggacttgat
     6601 aaattcattc ctaaatatga tacatttatt tttgtcatat ttaattaatt ttaaaattat
     6661 aattgcaaaa ataattagaa tttttatttt acatttcttt tgctaaaaaa taaagacttt
     6721 aagtttgcca aaaaattttt gtaataaaat taatttattt taatgattct tttggcaata
     6781 attctttcaa attatgattt tcttcttcca attgttctat tcttctttcc atttgttcca
     6841 tatacaaata taactcttca atattttgaa taattccttt gatagtttca taaacgggca
     6901 taccattgtc ctttatgtca gttgacgaag gtataaaagg taggtgtttt tgagatctta
     6961 ttaattctat tctttcatta aataactgaa gctgaaatga agaatcaaaa acgaaatctg
     7021 gccattggca agatgaatca cttggttcaa ctattatttc ttgagtagac ctaatagtac
     7081 cgcaaacatc taatttgtaa actggcgatg tattagctat acctacatta ccatttgaat
     7141 ttaacctaaa tccttcgttt ttattagttt taaaaattaa tggcatatta ttagttgtcc
     7201 caataaattc tgcttcatat atagcatttc catttaattc ccaactattt gaaccttgag
     7261 cacagcagtt ttgaatgctt gccatactaa taaaaagtaa agtattaata aaaataaata
     7321 atttaataaa tttgtttttt ataactttta tcgaattacc tatcaatata ttactgtaat
     7381 tctcaattat attatgcact tttttacgta taatcatttt atatacttat tttttataaa
     7441 attaatcata ttttttattt tttactttta ttttttttaa aaaaattaga tttattctcg
     7501 gcgaactcaa tattataagt agtaaggggc atttatttaa atggccacag tatattgtgt
     7561 agttaagaaa attgaaaaag atataatgta tatctgttaa aatttataca ggattaatat
     7621 cagaaaattt attaaatttg
//
LOCUS       DAOPRN010000080         7561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_8084_length_7561_cov_114.253930, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000080 DAOPRN010000000
VERSION     DAOPRN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8084_length_7561_cov_114.253930"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..3217
                     /gene="polA"
                     /locus_tag="PKI65_07630"
     CDS             407..3217
                     /gene="polA"
                     /locus_tag="PKI65_07630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOB78340.1"
                     /translation="MNKIGGNNLPYNHNALVIVDAMAMAFRYYYALIRRPMRTSYGLN
                     TSAVYGFTNMLLDILLKYTPRYLAIAFDTEEETLRKKEFDFYKAQRPPMPDELAQNIP
                     YIKKFIDALGIKRIEMPGYEADDIIGFISQKAAEHGIYSYIVTSDKDMGQLVNDKIFI
                     LKPAKNNEPDEILGVKEICEKWNISHPQQIIDILAIMGDASDNIPGVPGIGEKGAIKL
                     ISQFGSVENILANLEEVPERYRKLIIENVELLKDSKRLASINPHINIDFDIKDYEWKI
                     PPLDPIFNLCDELEFRTIKKRIPEVFKNLSDIYPIISNGQLSIFDDSDTITPLYQNDD
                     RLTKYNPKEQRYRLLIDKDEINNFVNKLEKQDFFVFDIETTSLDPLMANVVGISFALA
                     PYNAVFIYCKEKNICQFTLDLLKPIFENKKIAKGGFNLKYDLSILKQNNINLKGEIRD
                     AMVAHYILNPEGKHNMDYLADIYLDYKTITFNELIHEQKDFNLWDIDTKQLCIYACED
                     ADITLQLWEIFEKELKEKQQYDLYLNIESRLIPVLATMELNGVKIDLVFLKKFELDLQ
                     NQIDKLAKDIYELACEVFNISSPQQLGKILFEKLKITDKPKLTKTKQYSTSEEELLKY
                     QNQHPIINKILDYRELTKLLNTYVSAFPKLVNPKTGRVHANFNQTVTSTGRLSSSNPN
                     LQNIPVRTELGREMRRAFIPSTDDGYIVSADYNQIELRLMAALSKDKNMISDFVNDMD
                     IHAATAMRIFNLNKEDLTPEYRRKAKAINFGIIYGISAFGLSQQLGISRKEAKQIIDD
                     YFTKYPSVKQYMDKTIAFAKQHGYVETLFHRRRYLPDINAANAIVRGYAERNAINMPI
                     QGTAADIIKIAMIRVQDLISDNNLKSKLIIQVHDELVLDVVKEELDIITTNVKEIMEN
                     VIMLSIPLKVDIGFGNNWLEAH"
     gene            3431..4756
                     /locus_tag="PKI65_07635"
     CDS             3431..4756
                     /locus_tag="PKI65_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB78341.1"
                     /translation="MKKLVFTIFVLMMSLTMFGQVFESNFETWTDGSYPDGWGGTGTN
                     IGVANVVKYTTNAYAGNNSCQLINATTSHKRFTTTAVPIESGTAYKVEFYAKGKGDIR
                     LGIKRTASIASYMTYTAYTSINSSSWEMYTLTIVADTTSSDAEFILSIRNTDASMEHL
                     VIDNVTISVDTDIDEVSIYDIQYTTDASGDSPYNGQTIKTRGVVTAVTPNGFWLQSGK
                     GAWNGVYVYSNTINPNLGDSILISATVSEYYSLTELTNVALLENFGPSTIPDPEIITI
                     AQVGEPYEGVLCKLVNVICTNVDAGYGMWQITQSGNNLYVDDDIYAFTPTLNTHYVIT
                     GIIHFSFDDWKILPRNADDVVIYEGIENTENNNINLYYDANSKNIIVKGLQTDADLEV
                     INILGQNLITFNLSPSNINKVDISNFNSGIYILNLHNKNNIITKKIIVP"
     gene            5065..5787
                     /locus_tag="PKI65_07640"
     CDS             5065..5787
                     /locus_tag="PKI65_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOB78342.1"
                     /translation="MYREKEINEEEILKNAFKPKTWQEIKTHDSWEIFQILSEFVQGF
                     DKLASIGPCVTIFGSARAKPGSHHYKLAEEIAYLLTKKGFGIITGGGPGIMEAANKGA
                     HFAGGISVGLNIELPHEQKHNEFIDPDKLITFKHFFSRKVMFIKYAQGYIVLPGGFGT
                     CDEMFEALTLIQTKKSLRFPIVLVGKEYYKGLIDWLKETVLYNEYISDSDLCLFSLVD
                     TAEEAVDVIDKFYQKYDLKPNL"
     gene            5800..6399
                     /locus_tag="PKI65_07645"
     CDS             5800..6399
                     /locus_tag="PKI65_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOB78343.1"
                     /translation="MPLSTDFYLRNNVIEIAKSLIGKVIITQKDGYLTSGIITETEAY
                     NGIKDKASHAYGGKKTKRNQAMYMKGGHAYVYLCYGIHHLFNIVTNIEGIPDAILVRA
                     IYPLEGLEIMQHRAKMKKINANGVGPGKLTAMLSITTNDNTKSLINSDVIIEDYGFKV
                     PDNQIIATTRVGIDYAGEDARLPYRFLFPHDKINQLFKI"
     gene            6499..7158
                     /locus_tag="PKI65_07650"
     CDS             6499..7158
                     /locus_tag="PKI65_07650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOB78344.1"
                     /translation="MINEDFYLLLENQYDFITNDPIFYIIIRNTWVKTAYPQMLTHPD
                     TAAFLYILSKNKNINKVLDIGTFTGFSAILFARSTSNNAIIYTIEKHKEYENLINEHI
                     ELANVKSKIKIFYDDALLRLPHINEKFDLIYVDADKINYPRYLDLCLKLLNENGIIIF
                     DNINWYKNVESNKPDKKTKAIKELIDKVNKKSFLNNFQKLILPLGDSILILQKNQLKI
                     K"
BASE COUNT         2917 a          914 c         1169 g         2561 t
ORIGIN      
        1 tataatgctc tatggttagg agctacatat cgtagtttta gagaagctta taaagaaata
       61 tttgaaaata aaaaatgatt tgtcaaatat aataccgagc ctatcaatgt catactatat
      121 ttttagaaat caaatgattt attaattttt ctatttagtt aatataaaat aatagtctaa
      181 actttagatt tttaaatgat aaactagata tgtcttgctt aaaaaaaata ttaataactt
      241 tgatataatc ataaatggtt tactcagtca gctccattag atcattgaga agtatatttg
      301 acaaaataaa aataattaaa ttgaatataa atatgtattt aacatgatat ttataaataa
      361 gtataaagaa taaatttata ttttttaact ttgaaaaaat aataagatga ataaaatagg
      421 cggaaataat ttaccataca atcataatgc tttagtgata gtagatgcga tggctatggc
      481 ttttcgttac tattacgctc tgatacgtag acctatgcgc acaagttatg gattaaatac
      541 gagtgctgtt tatgggttta ctaatatgtt attagatatt ttattaaaat atacaccacg
      601 atatttagct atagcttttg atacagaaga ggagacatta agaaaaaaag aatttgactt
      661 ttacaaagca caaaggccac cgatgcctga tgaattagct caaaatattc cttatattaa
      721 aaaatttatc gatgctttag gtatcaaaag aatagaaatg ccaggatatg aagccgatga
      781 tattataggt tttatttctc aaaaagctgc tgagcatggt atatattctt acatcgtaac
      841 tagtgacaag gatatggggc agctagtaaa tgataaaatt tttattctaa aaccagctaa
      901 aaataatgaa cccgatgaga tattaggtgt aaaagaaatt tgtgaaaaat ggaatatatc
      961 tcatccacag caaatcatag atatactggc tattatgggt gatgcatctg ataatatacc
     1021 tggagtgcca ggtataggtg aaaaaggtgc tatcaaatta atatctcaat tcggctcagt
     1081 cgaaaatata ttagctaatc tagaagaagt acctgagaga tatagaaaat taatcatcga
     1141 aaatgtagaa ttactcaaag atagcaaaag attagcatcc atcaatcctc acattaatat
     1201 agatttcgac ataaaagatt atgaatggaa aataccacct ttagatccta tttttaattt
     1261 atgtgatgaa ttagaatttc gtactattaa aaaaagaatt cctgaggttt tcaaaaatct
     1321 atctgatatt tatcctatta tttctaatgg acagttatct attttcgatg attctgatac
     1381 tataacccct ctttatcaaa atgacgatag attgacaaaa tataatccta aagagcaaag
     1441 atatagattg ctaattgata aagatgaaat taataatttt gtaaataaat tagaaaaaca
     1501 agattttttt gtttttgata tagaaaccac aagtttagat cctttaatgg caaatgtagt
     1561 tggtattagc tttgcattag ctccttataa tgcagttttt atttattgca aagaaaaaaa
     1621 tatttgtcaa tttaccttag atttattaaa accgattttc gaaaataaaa aaatagctaa
     1681 aggtggtttt aatcttaaat atgaccttag catactaaaa caaaataata taaatctaaa
     1741 aggagaaata cgtgatgcta tggtagcaca ttatattcta aatccagaag gtaagcacaa
     1801 tatggattat ttagcagaca tctacctgga ttataaaact attactttta atgaacttat
     1861 acatgagcaa aaagatttca acctttggga tatagatact aagcaattgt gcatttatgc
     1921 ttgtgaagat gctgatataa ctctgcaatt atgggaaata ttcgaaaaag aacttaaaga
     1981 aaaacaacaa tatgatcttt atctaaacat cgaatctaga cttattccag ttttagctac
     2041 aatggaattg aatggtgtaa aaatagattt agtttttttg aaaaaatttg aattagactt
     2101 gcaaaatcaa atagataaat tagctaaaga tatatatgag ctcgcttgtg aagttttcaa
     2161 tatttcgagt ccacagcagt taggcaaaat cttatttgaa aagctaaaaa tcacagataa
     2221 acctaaattg actaaaacta aacaatatag tacatctgaa gaggaattgc taaaatatca
     2281 aaatcaacac cctattatca ataagatact tgactatagg gaattaacaa aattattgaa
     2341 tacttatgta agtgcctttc ctaaattagt aaatcctaaa actggtaggg tacatgctaa
     2401 ttttaatcaa actgtaacat ctactggtag gcttagttca tctaatccaa atttacaaaa
     2461 catccctgtg cgtactgagc ttggtagaga aatgcgtaga gcttttatac catctacaga
     2521 tgacggatat atagtatctg cagattataa tcagatagag cttagattaa tggctgcact
     2581 gagcaaagac aaaaatatga taagtgattt tgtcaacgat atggatatac atgcagcaac
     2641 agctatgcgt atttttaatt tgaacaaaga agatttaaca ccagaatatc gtagaaaagc
     2701 taaagctatt aatttcggta ttatttatgg tatctctgct tttggacttt cgcagcagct
     2761 cggtatcagt agaaaagaag ctaagcaaat aatagatgat tattttacta aatatcctag
     2821 tgtaaagcaa tatatggata agacaatagc ttttgctaaa caacatggtt atgtagaaac
     2881 tttatttcat cgcagacgtt atctacctga tatcaatgcc gctaatgcta tagtacgtgg
     2941 ctatgctgag agaaatgcaa taaatatgcc tatacaaggt actgctgctg atattatcaa
     3001 gatagctatg ataagggtgc aggatttgat aagtgataat aatctgaaat ctaaattaat
     3061 aattcaggtg cacgatgagc tagtattaga tgtggtaaaa gaagagttag atataataac
     3121 aacaaatgta aaagaaataa tggaaaatgt tataatgcta tctattccat taaaggtaga
     3181 cataggcttt ggcaataatt ggctagaagc tcattaaaat caaacaataa aatctaagag
     3241 aatgtttttt tgtgttttta taatgttatt ctgaatgtga agaggtaaac gatttaaatt
     3301 taaaaaacac atcgaaaaaa ttaaacttgt tatatttagc aatagcctgt ctaaaatatt
     3361 ttataatttt ttgcatatat taaaattttt taataatttt gtataattaa aattaaaaaa
     3421 aataaatgct atgaaaaaat tagtttttac aatttttgta ttaatgatga gtctgacaat
     3481 gtttggtcag gtttttgagt caaatttcga aacatggaca gatggttcat accctgatgg
     3541 atggggtggt actggtacta atatcggcgt agctaatgta gtgaaatata ctactaatgc
     3601 atatgcagga aataattctt gtcagttaat caatgctact acatctcata aaagatttac
     3661 tactactgca gtacctatag aaagtggtac agcttataaa gtagaatttt atgctaaagg
     3721 taaaggtgat attaggctag gtattaaacg tacagcatct atcgcttcat atatgaccta
     3781 tactgcatat acatctatta atagtagctc ttgggaaatg tatactctaa ctatcgtagc
     3841 tgatacgact agttctgatg cagagttcat actatccatt aggaatactg atgcttctat
     3901 ggaacatcta gtaatagata atgttactat ctccgtagat actgatatag atgaagtgtc
     3961 catatatgat atccaataca ctactgatgc tagtggagac agtccataca atggacaaac
     4021 gataaaaact cgtggtgtag taaccgccgt aacacctaat ggattttggc tgcaaagtgg
     4081 aaaaggtgca tggaatggcg tgtatgtata tagtaataca attaatccta atttaggcga
     4141 tagtatttta atttcagcta cagtatctga atattatagc ttaacagaac taacaaatgt
     4201 ggctttatta gaaaattttg ggccatctac tattccagat cctgaaataa tcactatagc
     4261 tcaagtagga gaaccttatg agggcgtttt atgcaaacta gtaaatgtaa tctgtacaaa
     4321 tgtagatgct ggatatggta tgtggcaaat aactcaaagt ggtaacaatc tatacgtaga
     4381 tgatgatatt tatgcattta ctcctactct gaatacacat tatgtaatta caggtattat
     4441 tcatttttcc tttgatgatt ggaaaatatt acctcgtaat gctgatgatg tggtgatata
     4501 tgaaggtata gaaaatactg aaaataataa tataaatcta tattatgatg ctaattctaa
     4561 aaatataatt gtaaaagggt tgcaaactga tgctgatcta gaggtaataa atatattagg
     4621 acaaaattta attactttta atttatcacc ttctaatatt aataaagttg acatctctaa
     4681 ttttaattct ggtatctata ttttaaactt acataataaa aataatataa ttactaaaaa
     4741 aattatagtt ccttaatttt ttataaccta attatattca ttctaaatgt tatttttctt
     4801 attaaagtga catttagagt gaatatgttt gtatgatttt tttatatata aatatttaaa
     4861 taagaattct aacatactgg taccagacaa taataaattt agagataaag attagatttt
     4921 tttttaactt ctaaactttt tattttttca tcaatatttg aatttgtttt cctgaaaaaa
     4981 agctttcaga ggctcgcaat aaataatttt cattctaagt attaaaaata tttttatgta
     5041 tatttgtaaa taaaaaaact atttatgtat agagaaaaag aaataaatga agaagaaata
     5101 ttaaaaaatg cgtttaaacc aaaaacttgg caagagataa aaacacatga ttcttgggaa
     5161 atttttcaaa tattgtcaga atttgttcaa ggttttgata agttagcatc cataggccca
     5221 tgtgtaacaa tttttgggtc tgcaagggca aaaccaggct ctcatcatta taaattagca
     5281 gaagaaatag catatctttt gaccaaaaaa gggtttggca tcattacagg tggtggacca
     5341 ggtattatgg aagctgcaaa taaaggtgct catttcgctg gtggtatttc tgttggtctt
     5401 aatatagaat tacctcacga acagaaacac aatgaattta ttgatccaga taaattgata
     5461 acatttaaac atttttttag tagaaaagtg atgtttatta aatatgctca aggatatatt
     5521 gttttgccag gtggctttgg cacttgtgat gaaatgtttg aagctctaac attgatacaa
     5581 actaaaaagt ctttgcgttt tcctatagtt ttagtaggaa aagaatatta taaaggatta
     5641 atagattggc tcaaagaaac tgtattatat aatgaatata ttagtgatag cgatttatgc
     5701 ttattttcat tggtagatac agctgaagag gccgtagatg ttattgataa attttatcaa
     5761 aaatatgatc tcaaacctaa tctataattt tttttaataa tgcccctttc tacagatttt
     5821 tatttaagaa ataatgtaat agaaatagct aaatcattaa taggtaaggt gataataacc
     5881 caaaaagatg gatatctaac ctctggaatc atcacagaaa ctgaagcata taatggaatc
     5941 aaagataaag cttcacatgc ttatggaggg aaaaaaacaa aaagaaatca agctatgtat
     6001 atgaaaggtg gacatgctta tgtttactta tgttatggta tacatcattt atttaatatt
     6061 gttactaata ttgaaggaat tcctgacgca atacttgtaa gagctattta tccattagaa
     6121 ggattagaaa ttatgcaaca tagagcaaaa atgaaaaaaa taaatgctaa tggagtaggg
     6181 cctggtaaat tgacagctat gttgtccatt actactaatg ataatactaa aagtctaata
     6241 aattctgatg ttattatcga agattatgga tttaaagtac cagataatca aataattgca
     6301 actactcgtg taggtataga ttatgctggt gaagatgctc gtttgcccta tagatttttg
     6361 tttccacatg acaagattaa tcaattattt aaaatataaa tatagttgtt attttgttaa
     6421 atttataaca tctatattgc aattttagag gtatatttta tgtttttgac tgattataat
     6481 tattttttgt gattagctat gataaatgag gatttttatt tattactcga aaatcaatat
     6541 gattttatca ctaatgaccc tattttttat ataattataa gaaatacatg ggtcaaaact
     6601 gcatacccac agatgcttac tcatccagat acagctgctt ttctttatat tttatctaaa
     6661 aacaaaaaca tcaataaagt attagatatt ggtacgttta caggattttc agcaattctt
     6721 tttgctagga gtactagtaa taatgcaatt atttatacga tagaaaagca taaagaatat
     6781 gaaaatttaa taaatgaaca tatagagtta gctaatgtaa aaagtaaaat aaaaatattt
     6841 tatgatgatg ctctactaag actgccacat attaatgaaa aatttgattt aatatatgtg
     6901 gatgctgaca aaatcaacta tccacgatat ttggatttat gtttaaaatt attaaatgaa
     6961 aatggcataa taatctttga taatattaat tggtacaaaa acgtagagtc aaataaacca
     7021 gataaaaaaa caaaagctat caaagaatta atagataaag ttaacaaaaa atcttttttg
     7081 aataattttc aaaaacttat tttgccatta ggtgatagta ttctaatatt acaaaagaat
     7141 caattaaaaa taaaataaac aaaattttgt gtgttgagat cctaaatatc atacataaca
     7201 caaggcaatt aaaactaaaa aagttttaat atcctcaata aattattttt tgtaattgcg
     7261 atctctagat tttaggttag aatttatggt tctataatgt ttattgtggg taaaaatttt
     7321 agattgttac aaaaaaatca ttaagagata cgataattac gattataaag ctaattcggg
     7381 ttgtattaaa taatagcgaa taaaagaaat tagatgaaaa actaataaat gtttaagttt
     7441 ttcaagttta tatgttaaat aatttttata tttttacaaa aattttaatt catgaaaaaa
     7501 atagtggaaa acaccagtaa ataccttgct atgagtaagg aagagaataa agaattctta
     7561 g
//
LOCUS       DAOPRN010000081         7455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_8218_length_7455_cov_103.943378, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000081 DAOPRN010000000
VERSION     DAOPRN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8218_length_7455_cov_103.943378"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1558
                     /gene="mtaB"
                     /locus_tag="PKI65_07655"
     CDS             275..1558
                     /gene="mtaB"
                     /locus_tag="PKI65_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOB78345.1"
                     /translation="MAVNKRVAIHTLGCKLNFAESSSILNKFRQRQYEIVSFDDDADI
                     YVIHSCTVTATADKKTRQLINKVKNANPKAKIALIGCMVDNLSQNSSILEKTDWALGN
                     KEKMFLPEIIENEAINDSIFNSRFIPAFSTYDRTRSFLKIQDGCDYFCEYCTIPYVRG
                     RSRSAEIKDILNQLDFLVDKGIKEVIFTGINIGDYGVNQNFSFADLLYTISKQSYPLR
                     FRISSIEPNLLSDEIIELVASSDAFMPHFHIPLQHGSDRILQSMKRRYTIDYFVKLIY
                     KIKKSIPDAFIANDVIVGFPDETDEDFRNMFDLLKDLPIAYLHVFPYSDRPLANSFNF
                     KNKIPANIKKQRSKILTKLSKEKQENFYNSFLNTTSKVLWEAKIEDDFIYGYTENYIR
                     VATHYDANLINKISNVLLEKLDKNIIIAKILNDEK"
     gene            1545..2357
                     /locus_tag="PKI65_07660"
     CDS             1545..2357
                     /locus_tag="PKI65_07660"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOB78346.1"
                     /translation="MMKNKSLVEYLLPHVLGLVEEVGEFIISERRNFDHNKVEEKTPG
                     NLVSYVDKTAESRLIDGLSVLLPNSTFLSEEFNSVEILDELTWIIDPLDGTTNFVHNF
                     PLYTISIALTIEQELMLGIVYDPIHKEMFHAIKSLGAFLNNKTIKVSQTPDLYSSLWA
                     TGFPYQEFDKLKNYVDFFYHLIKISHGVRRLGSAAADLAWTACGRVDGFYEYGLCPWD
                     VAGGIIIVQEAGGKITNFTGGEEALFAKEIIASNGLIHDEFFENFEKIYFSI"
     gene            2624..3133
                     /locus_tag="PKI65_07665"
     CDS             2624..3133
                     /locus_tag="PKI65_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78347.1"
                     /translation="MNHNLSKIIFLVILIMTFGSCKKINTSQVIEYNNQIVNDQKLIL
                     QYESKLYNAIYDNANIDTLKSILKEYQLIIDSINSKYQNPFKIEDTMRKVALSLFDAY
                     KSIAYNEYPYLISLIDSANKGAIIDSDEIMKILVTIDSTEQTANMSYIDLQQYIVNKY
                     KIDLINSTK"
     gene            3149..3898
                     /gene="gpmA"
                     /locus_tag="PKI65_07670"
     CDS             3149..3898
                     /gene="gpmA"
                     /locus_tag="PKI65_07670"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOB78348.1"
                     /translation="MKTLVLVRHGESVYNRANIFTGWTDVPLTQQGELEAIEAAKVLK
                     ENGFDFDITYTSVLNRAIKTLWIILEQMDRMWLPEIKTWRLNEKHYGKLQGLNKAEMA
                     AKYGEEQVLLWRRAYDVRPPLLSEDEIAEMRKEARYADVDNIQYMLGESLKDTVERVI
                     PLWENQIKYDLIADKRVLISAHGNSLRGVVKYLDQMSEDEILHFNIPTGIPLVYELDN
                     NLEPKKRYFLADEEKLKTAIDKVANQGKAKN"
     gene            3901..5295
                     /gene="glmM"
                     /locus_tag="PKI65_07675"
     CDS             3901..5295
                     /gene="glmM"
                     /locus_tag="PKI65_07675"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOB78349.1"
                     /translation="MSLIKSISGIRGTIGDADGLDPITVVKYTLAFASVINKKYPNIT
                     PSILVGRDGRVSGNAIKQLVINSLICSGINVYDADYSTTPTLEMAIPHLNCQAGIMIT
                     ASHNPQNWNALKFFDENGEFITEQILKEIEYIANNNEDFYLVKEQYFGQVYSATDIID
                     YHIKKIIELPYIKTQLIKESNFKIIVDTINSTGSIAIKKLFDALGIENYTFLNEEVNG
                     LFEHNPEPLPENLSQLSFTIKMNKANVGFAVDPDVDRLAIVCEDGEMFGEEYTIVAIA
                     DYVLEKKPGPVVVNMSTTQAVKYVADKYNCPIYYTPVGEINVVNKMKEVGAVIGGEGN
                     GGVILPELHYGRDAILGIALFLSFLAEKQKPVSVIRSYYPNYYMIKSKIETKDMNITV
                     DKILDEIQKRNSHLKFINIDGIKFMDDDDTWIHLRPSNTEPIIRIYIESSNQTKCESF
                     LYKIMQDINDILKQ"
     gene            5299..5565
                     /locus_tag="PKI65_07680"
     CDS             5299..5565
                     /locus_tag="PKI65_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78350.1"
                     /translation="MPRKKSKFKKYNFSISYKTAKKFEEYCNMCGYTPNKFIKTVIKD
                     FIQNNDHHTLHYFYKKENSDVKLLNNKTQTSKSNVNQLSLFDDF"
     gene            complement(5657..7252)
                     /locus_tag="PKI65_07685"
     CDS             complement(5657..7252)
                     /locus_tag="PKI65_07685"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOB78351.1"
                     /translation="MDAVVIITILLFLACVVGLGLIFRKANYNFWFVFVPVYNLVVWA
                     KIIGKKWSWVAWSLVPFINIFVMMLMMIELLKGFNRYTILNQVLIILFPFVYLPYLGL
                     IKDGYIPVEEQKVKKLTKTQEWIEAIIFAVVAATIIRMFFLEAYTIPTSSMEKTMLVG
                     DYLFVSKIHYGIRIPNTPLAFPFAHNIMPLTKTKPSYSKLIQLPYYRYPSLEKIESNK
                     LIVFNFPEGDTVIIAEPAMSFYTIARSFAVQMAKSNGLTEKNYQKFYDAARKYLLQTE
                     KITVRPVDKRDNYIKRCIGTPGDTIQIIHRKVFVNGKPMPFSKYIQFDYIAKLQPDRY
                     LSIEQIMSTGISREDARQSAPKAQYYGYPPNVLILPMTDEIKDKFLKKFGAILAEPLK
                     ISEDTTWDYSIFPHDPKYPWNKDNFGPIVVPKKGMSVNIDTNNLCFYERIITAYEHNK
                     LYVQNGSIFINDQPTNTYTFQMNYYFGMGDNRHNSYDSRFWGFIPEDHMVGKPVFVWL
                     SLDKDYSLFDGKIRWKKLFRVPK"
     gene            complement(7370..7443)
                     /locus_tag="PKI65_07690"
     tRNA            complement(7370..7443)
                     /locus_tag="PKI65_07690"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7407..7409),aa:Pro,seq:ggg)
BASE COUNT         2761 a         1022 c         1157 g         2515 t
ORIGIN      
        1 gcgagcgtgg tatgtataca gagacttgag agcaagtcct gtgtatattt aaacatttaa
       61 aaatgttatc attgcccaat ccaaaaaaat ttcgctgtta ttctgaacac taacaatttt
      121 tcttaaatca ttatatgttt tggtgtaaca taaaggtcga cattctttta acacttttaa
      181 tgttctattt acattttaaa gaatacaatg tgatagtttt attttttttt ataagtagtt
      241 atttttttta actttgcaat aaataaaaag tttaatggct gtaaataaaa gggttgctat
      301 tcacacatta ggttgtaagc taaattttgc agaatcatca tctatattaa ataaatttcg
      361 tcaaagacag tacgaaatcg tttcttttga tgatgatgca gatatttatg tgattcacag
      421 ttgtacagtt acagctactg ctgataaaaa aactcgacag ctaataaaca aggttaaaaa
      481 tgccaatcca aaagctaaaa tagcccttat tggttgtatg gtagataatt tatctcaaaa
      541 ttcttcaatt ttagaaaaga ctgattgggc cttaggtaat aaagaaaaga tgtttttacc
      601 agaaatcatt gaaaatgaag caataaatga ttcgattttc aactctcgat ttattccagc
      661 ttttagtact tatgatagaa ctagatcttt tctaaaaatt caagatggtt gcgattattt
      721 ttgtgaatat tgtactatcc catatgtgag aggtaggagt cgtagtgctg agataaaaga
      781 catattaaat caattagact ttttagtaga taaaggtata aaagaagtaa tctttacagg
      841 aataaatata ggtgactacg gtgtaaatca aaattttagt tttgcagatt tgctttatac
      901 tataagcaag caatcttatc cattaagatt tcgcattagc tctatcgaac cgaatttatt
      961 aagcgatgaa ataatagagc ttgtagcatc atcagatgct tttatgccac attttcatat
     1021 acccttacag catggtagtg ataggatatt gcaatcaatg aaacgtagat atacaatcga
     1081 ttattttgta aaattgattt ataaaattaa aaaatcaata cctgatgcct ttatagctaa
     1141 tgatgtgata gttggtttcc ccgatgaaac tgatgaggat tttcgaaata tgttcgatct
     1201 tttaaaagat ttacctatag catacctaca cgtatttcct tacagtgata gaccattagc
     1261 taattctttt aattttaaaa ataaaatacc agctaatata aaaaaacaaa gatcaaagat
     1321 attaacaaaa ctaagtaaag aaaaacaaga aaatttttac aatagtttct taaatactac
     1381 atctaaagtg ctatgggaag ctaaaattga agatgatttt atatatggat atactgaaaa
     1441 ttatatccga gtagctactc attatgacgc taatctaatc aataaaattt caaatgtact
     1501 tttagaaaaa ttagataaaa atataataat agcaaaaata ttaaatgatg aaaaataaaa
     1561 gtttggtaga atatttattg ccacatgtac ttggattagt agaagaagtc ggagaattta
     1621 taatatcaga aagacgtaat tttgatcata ataaagtcga ggaaaaaact ccaggtaatt
     1681 tagtatctta tgtagacaaa actgctgaaa gcagattgat agatggacta tcagtgttat
     1741 tacccaatag tacttttcta agtgaggagt ttaatagtgt agaaatattg gatgagctta
     1801 cttggatcat cgatccatta gatggtacta ccaattttgt tcataatttt cctttatata
     1861 ctattagtat agctctaact atagagcaag aattaatgtt gggcatagtt tatgatccta
     1921 tacacaaaga gatgtttcat gctattaaaa gtctcggagc ttttttaaat aataaaacta
     1981 taaaagtatc acaaactcca gatctctata gctcattgtg ggctacaggt tttccttatc
     2041 aagagtttga caaattaaaa aattatgtgg atttctttta tcatcttatc aaaatatctc
     2101 atggagtgcg tagattaggt agtgctgctg ctgatctggc atggacagct tgtggcagag
     2161 tagatggctt ttatgaatat ggtttatgtc cttgggatgt ggctggtggc attattattg
     2221 tacaagaagc tggtggtaaa attactaact tcactggtgg cgaggaagct ttatttgcta
     2281 aagaaataat agcaagcaat ggattgatac atgatgaatt ttttgaaaat ttcgaaaaaa
     2341 tatattttag tatataaaat aaagcaacaa tctgaagaaa tcataatatt tatgtgaaag
     2401 ttattattaa ttaaacttca aaatttttgt aacgcgtagc ttatttatac ttttttataa
     2461 attaatataa taaattactt atttttttat ttaaaatctc gtattcttca ttgtcaattg
     2521 taatttttta attgtcattt aaatattgaa atactctaac tatttatata tttattattt
     2581 tttattatct ttttttattt atctttgtaa aaaatttata atcatgaatc ataatttgtc
     2641 gaaaataatc tttttagtca ttttaataat gacatttggc tcttgcaaaa aaataaatac
     2701 tagccaagtg atagaatata ataatcagat agtaaatgat caaaaactta ttttacaata
     2761 tgaatctaaa ctctataatg ctatttatga taatgcaaat attgatactt taaaatctat
     2821 cttgaaagag taccagctaa ttattgactc tatcaatagt aaatatcaaa acccttttaa
     2881 aatagaagac acaatgcgaa aagtcgcttt atcacttttc gatgcataca aaagtattgc
     2941 ttataatgaa tatccttatt taatatcact tatagatagt gcaaataaag gtgctataat
     3001 tgatagcgat gaaattatga aaattttagt tacaatagat agtacggagc aaactgctaa
     3061 catgagttat attgatttac aacaatatat tgtaaataaa tacaaaattg atttaataaa
     3121 ctcaactaaa taacaactta attaatatat gaaaacatta gtccttgtca ggcatggtga
     3181 aagtgtatat aatcgtgcca atatatttac agggtggaca gacgtaccat taactcaaca
     3241 aggtgagcta gaagcaatag aagctgctaa agtattgaaa gaaaatggat ttgactttga
     3301 tatcacatat acttctgtac tgaatagagc tatcaaaaca ctttggatta ttttagaaca
     3361 aatggataga atgtggttac cagaaataaa aacttggcga ctgaatgaaa aacattatgg
     3421 caaattacaa ggcctcaata aagctgaaat ggcagctaaa tatggtgaag aacaagtatt
     3481 gctttggcga agagcttatg atgtgagacc tccattatta tcagaggacg agatagctga
     3541 gatgagaaaa gaagctagat atgcagatgt agataatata caatatatgc ttggtgaatc
     3601 actaaaagac acagtagaaa gagtgatccc actttgggaa aatcagataa aatatgatct
     3661 aatagcagac aaacgtgtct taatatctgc acatggtaat agtctgagag gagtagtaaa
     3721 atatctagac caaatgagtg aagatgaaat attacacttc aatattccta cagggattcc
     3781 cttagtatat gaattagata ataatctcga gcctaaaaaa agatatttct tggctgatga
     3841 agaaaaattg aaaactgcga tagataaagt cgctaatcaa ggtaaagcta aaaattaatt
     3901 atgtcactta taaaatcaat ttcaggaatt agaggtacta ttggtgatgc agatggttta
     3961 gatcctataa ccgtagttaa atatacattg gcttttgcta gtgttattaa taaaaaatat
     4021 ccaaatatca ctcctagtat tttagttggt agagacggta gagtttctgg taatgctata
     4081 aagcaactag taataaatag tttgatttgt tcaggaataa atgtttatga tgctgactat
     4141 agtaccacac ctactttaga aatggctatt ccacacctca attgccaggc tggtatcatg
     4201 ataactgcta gccataaccc tcaaaattgg aacgctctta aattttttga tgagaatggt
     4261 gaatttatca ctgaacaaat attaaaagaa atagaatata tagctaataa taatgaagat
     4321 ttttatttag taaaagaaca atattttggt caagtttatt ctgctacaga tattatagat
     4381 tatcatataa aaaaaatcat tgaactacca tatattaaga ctcaattaat aaaagaatct
     4441 aattttaaaa ttatcgttga tactattaac agtactggaa gtatagctat taaaaaactt
     4501 tttgatgctt tagggatcga aaattatact tttttaaacg aagaagtaaa tggacttttt
     4561 gagcataatc cagaaccatt accagaaaat ctttcacaat tatcttttac tattaaaatg
     4621 aacaaagcta atgtcggttt tgctgttgat cccgatgttg atagattagc tattgtatgt
     4681 gaggatggtg aaatgtttgg cgaagaatat accatcgtag ctattgccga ttatgtttta
     4741 gaaaaaaaac cgggtccagt tgtcgtaaac atgagtacta cacaagctgt aaaatatgtt
     4801 gcagacaagt ataattgtcc tatttattat actcctgttg gggagataaa tgtagtgaat
     4861 aagatgaaag aagtcggtgc agtcataggt ggagaaggga atggaggggt tatattacca
     4921 gaattgcatt atggtagaga tgccatatta ggcatagctc tttttcttag ttttttagca
     4981 gaaaaacaaa aacctgtatc tgtcatacga tcatattatc ctaattatta catgataaaa
     5041 tctaaaatag aaactaagga catgaatatt actgttgata aaatcttaga tgaaatacaa
     5101 aaaagaaatt cacatttaaa atttataaac atagatggaa ttaaatttat ggatgatgac
     5161 gatacatgga ttcatctgag accttctaat acagagccta tcattcgtat ctatatcgag
     5221 agtagtaacc aaactaaatg tgaaagcttt ttgtacaaaa tcatgcaaga tattaatgat
     5281 atattaaaac aatagactat gcctagaaaa aaatctaaat ttaaaaaata taatttttct
     5341 atttcatata aaacagcaaa aaaatttgaa gaatattgta atatgtgtgg ctacactcct
     5401 aataagttta taaaaactgt aattaaagat tttattcaaa ataatgatca tcatacatta
     5461 cattatttct ataaaaaaga aaattctgac gttaaattat taaataataa aactcaaaca
     5521 tcaaaaagca atgttaacca actatcttta tttgatgatt tttgaaaaaa taatgtatgt
     5581 cgaatctcta ctcttgactt tctcgtgatt tattggaata tatatataat gtgcaatatt
     5641 ttttttcaat ctagtttcac ttcggtactc tgaaaagttt tttccatctg attttaccat
     5701 cgaatagaga ataatctttg tctagagata accatacaaa aactggctta cccaccatat
     5761 gatcttctgg tatgaagccc cagaatctcg aatcatagct attgtgccta ttatctccca
     5821 taccaaaata ataattcatc tgaaaagtat acgtattggt gggttgatca ttgataaaga
     5881 ttgagccatt ttgcacatat aatttattgt gttcataagc tgtaatgata cgttcgtaaa
     5941 agcataaatt atttgtatct atattgacgc tcataccttt tttaggcact acaatagggc
     6001 cgaaattatc tttattccat ggatatttag gatcgtgagg aaagatagaa tagtcccaag
     6061 tagtgtcttc acttatcttt aaaggttcag ctaagatagc accgaatttc tttaaaaatt
     6121 tatcttttat ctcatcagtc attggtaaaa ttaaaacatt agggggataa ccataatatt
     6181 gagcttttgg agcagattgt ctggcatctt ctctagaaat ccccgttgac attatttgct
     6241 caatagacaa atatctatca ggttgtagct tagcaatgta atcaaattga atatatttag
     6301 aaaaaggcat aggtttacca tttacaaaaa cttttctatg aattatctga atagtatctc
     6361 caggggtacc aatgcatctt ttaatgtaat tatctctttt atctactggg cgaactgtta
     6421 tcttttctgt ttgcaataaa tatttgcgag cagcatcata aaatttctga taattttttt
     6481 ctgtaagccc attactttta gccatttgta cagcaaaact tctggcaata gtataaaagc
     6541 tcattgcagg ctcagctatg atcacagtat caccttctgg gaaattaaat actattaact
     6601 tattactttc gattttttct aaagaaggat atcgataata aggcaattgt attaatttag
     6661 aataggatgg ttttgtctta gtaagtggca ttatattatg agcaaaaggg aaagcgagcg
     6721 gagtattagg aatacgaata ccataatgaa ttttacttac aaataaataa tcaccaacaa
     6781 gcatagtttt ttccattgaa cttgtaggta ttgtataagc ttctaagaaa aacattctaa
     6841 taattgtagc tgctaccaca gcaaagatta tggcttctat ccattcttga gttttagtta
     6901 gttttttaac tttttgctct tctactggaa tgtatccatc ctttataaga cctaaatatg
     6961 gtaaataaac aaacggaaaa agtatgatca aaacttgatt taatatagta tagcgattaa
     7021 aacccttcaa tagctctatc atcataagca tcataacaaa tatattaata aatggtacta
     7081 gactccatgc tacccagctc catttcttgc ctattatttt tgcccatact actaaattat
     7141 atactggtac aaaaacaaac caaaaattat aatttgcttt tctaaagatc aatccaagcc
     7201 cgacaacaca agctaagaaa agtaaaatgg ttataatgac aactgcatcc ataagattta
     7261 ttttttgaaa atattttgca aatataaatg taaaatttaa aatttgataa taaaataatg
     7321 attaatacaa aaaaaagata ggagaggttc atacatcctc tcctatctat cggagcggtc
     7381 cgacttgaac ggacgacccc ttgcacccca tgcaagtgcg ctagccaact gcgctacgcc
     7441 ccgtaaagta atttt
//
LOCUS       DAOPRN010000082         7450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_8226_length_7450_cov_120.817444, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000082 DAOPRN010000000
VERSION     DAOPRN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8226_length_7450_cov_120.817444"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            557..1612
                     /locus_tag="PKI65_07695"
     CDS             557..1612
                     /locus_tag="PKI65_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOB78352.1"
                     /translation="MKIGITADLHLKNANETKERYEALNQILNILQNQDISHLVIAGD
                     LFDKDVYNYNDFEKLCQNYSNINFHIIPGNHDAALDKRYFSLPNINVYSEITIKKFDN
                     ISFLFLPYRLSGTMDEALINYFNNYNLTDKWILVGHGDYLDISKSADEYEKGYYMPLS
                     RNIINELEPLRVFLGHIHKAYTKDKITYPGSPYPLDINETGKRKFLIYNTENDNIEDV
                     YLDIDKIYMVEELLIIPTNNEIDDAINKIDNLINNWNFNDVELNKVILRLILKGFTSD
                     KNALIHNITQHIVSKNIKFYDNNLPRDENLNVVKDPDREFIMKNILEKIDNINYDFVF
                     SEKQDVIEQALNLIYKD"
     gene            1632..4256
                     /locus_tag="PKI65_07700"
     CDS             1632..4256
                     /locus_tag="PKI65_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78353.1"
                     /translation="MSIRIQEINIDNLGPIKPGKYQFDNINLIYSKNEGGKSILVEFI
                     LRSLFSKKSIWGYNREIGNGKIIVSGINGKSISYSPASKDKLDKYFEKQLNTFQTSLT
                     KLLIIKSGETNINEGPQGIDVNTLKELLSSKNILDKIDSKIQKTIQNASFTNNFINID
                     NTGEGKTYKDLKDNQLQKINQLINRLNDNKINEELKLRNDIEELKVKKENLLKAKKFK
                     AYNLFNKKQELENILNDYPDDKLEELKKDINDYVKFKENIEKLENESKNISDEIKIFK
                     NLRADYEKQLNAKRNKAYKLDNKIKEFNLKLSKISDDDLNKLDNKIINYKEKSTDHQK
                     KSKELEKLEEKNRDFNWVKNIEKNYHDLKQNAYNQKVSITYLIVAAIVFISGLVVSII
                     NKNVIIGSVLMLLGFGFSIYFSLNLRKLLKNKAKNDELEKLENSFKEKFENELDDAYL
                     ATLISELDVSKLIDMVKDEQTKLLNEINRLKAEIENQLFQIFSKNIDEADWLIELNNY
                     KQERKKDDNTLKFLELELAKLEVDVTDYYTNDIDEEYDSRKLLDLQEKLNNLNILENQ
                     LKNIERELKNENDKLEKLKEKIQNVFDSLNIQIPENEWEGKLKELIKETTKIKNDINA
                     IAGELAGLGIDPDDFITEDPGIKFDPTKLLDLENLINEKSNQLKEIEKENLDYKGEIA
                     NITGLSIKDDWNILIDHLYEKQNEILNQLKHQESFIIAGILVHQAISELLRAEDEHIQ
                     NSINSPNFSDLLFNITGKYNNLLIDDGGKILISSDTAEYKLQDLSTGTKEQVLFALRV
                     TLAEKILDDKAFFILDDAFQHSDYDRRPKLIDQVFSLADKGWQIIYLTMDKHIQKLFS
                     DKASNRQDFNEIVLDRLS"
     gene            4477..5763
                     /locus_tag="PKI65_07705"
     CDS             4477..5763
                     /locus_tag="PKI65_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOB78354.1"
                     /translation="MTNQIKNIKENHNLENTKKLDFFSDHNTNELKENEQLSLLDNVQ
                     FIYELSLAQLELQSLGVYFEMTNSLRNFKLIETQNDDELLKKLAYFKSVKGNPTYYYQ
                     IIQKNRTRSVNQYLTHWIYPYKGKFHPQMIRALLNIIGLEQGDTVLDPYIGSGTTALE
                     AQLLGINCIGIDVSPLCVIQSKVKTESIDVFSEILEWKDEIIKNIEPSLFNMNGNTID
                     NTINIIPNERVRNFYKMAKLVSISDNARRGKDFKTAFVKNIEMMIASVSDYIEIKNDL
                     NLKLGNIDIKTGDSRALPLENNSIDGIITSPPYSIALDYVSNDAHALEELGYNLSEIR
                     EEFIGVRGKGRTRIDLYNDDMKNSLEEMYRVLKPNKYAAIVIGNATYMAQEVKTVEFT
                     INYAEKIGFKLVKNIDKIIFGLYNVMQKENILIFQK"
     gene            5771..6448
                     /locus_tag="PKI65_07710"
     CDS             5771..6448
                     /locus_tag="PKI65_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78355.1"
                     /translation="MTKKEQEKYIKELEETISQFIKPLKNIPFKIVLKAISECELIPF
                     DKNNPKDKQLLDDLIKAATIATNNAYEKGIFTRRENEVGNHIEPFVIDALNQIGLVAS
                     RPKTKEGKKKATGYPDIFLEDRNGRPNYIECKTYNERNYQTTQRSFYFSPAERTADFK
                     VIHDARHLVISFKIEKSERDGQMVFFPVYWKIFSLDSLIGQIKHEFNSTNKQLYRDEA
                     LLAEGSI"
     gene            6751..>7450
                     /locus_tag="PKI65_07715"
     CDS             6751..>7450
                     /locus_tag="PKI65_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78356.1"
                     /translation="METKEQKFYQALQDVFIGARIEGQGGFVNLMKIKSNYYSKIEKL
                     IKEDIEKALMQYPDFRNELFDKLYSFFSRYFSESGSIYFNSTPFHNNVYEKVYTDEKD
                     VILFWKTHMLYYVKTDRIFKSMEIEVDGFKFHFDVSELEHKKSNEKRNLIYELKEVRK
                     DKTLVFNVLYSERGRKTKQNEILRTLKKEKISIDEEVLEKAFRTFEKQSEVDFFINKN
                     AKAFLQEQFKLWSYQ"
BASE COUNT         3122 a          837 c         1109 g         2382 t
ORIGIN      
        1 actagataga caaaaaaagg ggggcatcat ttatttgatg ccccctcttt tttttgtgca
       61 ttaatgaatg tatataaagc cttttttatg tattttttaa taaagtatgc aattttttac
      121 taaatagctc aattaaatgt gatttaactt tttagtactt acctatcagg tgtgaaaata
      181 atttattata aaggtatatt atgaaaaagc acattaaagt accaagttgc tttaaatagt
      241 ttgcattgtt attatcatga ttttaatggg ttctataacg tttattgtgg gtaaattatt
      301 taggttattt ttttaccaca aagggcacca agatgataca ctttgtgcat tctttgagta
      361 ctctgtggtt aatattcaat tgtacaatga taaataaacc aatattaaca aatttttttg
      421 taaaattact tttttttaat ttttacctat ataaaacgtt atagaaccaa aaaatatctc
      481 ctaaatttta ttaggataat ataaaaacat tcacctcaca aaaattaatt tttatgtata
      541 tttgaaaaaa atatttatga aaataggaat aactgcagat ttacatttaa aaaatgcaaa
      601 tgaaaccaaa gaaagatatg aagcattaaa ccaaatttta aatattttac aaaatcaaga
      661 tatttctcat ctcgttatag ctggcgatct ttttgataaa gatgtatata attataatga
      721 tttcgaaaaa ttatgccaaa attattcaaa tattaatttt catattatac ctggcaatca
      781 cgatgctgca ctggataaaa gatattttag tttacctaat atcaatgttt atagtgaaat
      841 aacaataaaa aaatttgata atatttcttt tctgtttctt ccatatcgat tgtcgggcac
      901 aatggatgag gctttgatta attattttaa taattacaat ttaactgata aatggatctt
      961 agtaggtcat ggcgattatt tggatattag taaatctgct gatgaatatg agaaaggcta
     1021 ttacatgcct ttatctagaa atattataaa tgaattggag ccacttagag tttttttagg
     1081 acatattcac aaagcttata caaaggacaa aattacatat cctggctcac catacccact
     1141 tgatattaat gaaactggca aacgtaaatt tttgatttac aatacagaaa atgataacat
     1201 cgaagatgtt tatttagata tagacaagat ttatatggta gaggagcttt taataatacc
     1261 tactaataat gaaatcgatg atgcaataaa taagatcgat aacctaatta ataattggaa
     1321 ttttaatgat gtggaactaa ataaagtaat tttaaggtta atattaaaag gtttcacaag
     1381 tgataaaaat gcactcattc acaatataac tcaacatatc gtatctaaaa atattaaatt
     1441 ttatgataat aaccttccaa gagatgaaaa cctaaatgta gtaaaagatc cagatagaga
     1501 atttatcatg aaaaatattt tagaaaaaat agacaacata aattacgatt ttgttttttc
     1561 agaaaaacaa gatgtcatcg agcaagcatt gaatttaatt tataaagatt aattattaaa
     1621 aaaaaattat tatgtcgatt agaatacaag aaataaacat agataattta ggacctatta
     1681 agccgggaaa atatcaattt gataatatca atctaattta tagcaaaaat gaaggcggta
     1741 aatcaatatt agtagagttt attttgagat cattatttag taagaaatct atctggggtt
     1801 ataataggga aatagggaat ggtaaaatta ttgtttctgg tattaatggt aaatcaatca
     1861 gttattcgcc cgctagtaaa gataaattgg ataaatattt tgaaaaacaa ttaaatactt
     1921 ttcaaacttc attgactaaa cttttaatta ttaaaagtgg tgaaactaat ataaatgaag
     1981 gtccacaagg tattgatgta aatactttaa aagaattact ctcttctaaa aatatattag
     2041 acaagatcga ttcaaaaata caaaaaacta ttcagaatgc aagtttcact aataatttta
     2101 taaatattga taatactggc gaaggtaaaa cgtataaaga tttaaaagat aatcaattac
     2161 aaaaaatcaa tcaacttatt aatagattaa atgataataa aataaatgaa gagttaaaat
     2221 taaggaatga tatagaagag ttgaaggtaa agaaggaaaa tttactaaaa gcaaaaaaat
     2281 ttaaagcata taacttattt aataaaaaac aagaattaga aaatatatta aatgattatc
     2341 cagatgataa attagaggaa ttgaaaaaag atataaatga ttatgtaaaa tttaaagaaa
     2401 atatagagaa attagagaat gaatctaaaa atatatcaga tgaaataaaa atttttaaaa
     2461 atctaagagc tgattatgaa aaacaattaa atgctaaacg aaataaagca tataaactag
     2521 acaataaaat aaaagaattt aatctaaaat taagcaaaat ttctgatgat gatcttaata
     2581 aactagataa caaaataata aactacaaag aaaaatcaac tgatcatcaa aagaaatcaa
     2641 aagaattaga aaaattagaa gaaaaaaata gagatttcaa ctgggtaaaa aatatcgaaa
     2701 aaaattacca tgatttaaag caaaatgctt ataatcaaaa ggttagcatc acatatttga
     2761 ttgtagctgc tatagttttt atttcaggat tagtagtttc tataatcaat aaaaatgtta
     2821 ttattggcag cgtacttatg ctactaggtt tcggttttag tatttatttt tcacttaatc
     2881 tacggaaatt attaaaaaat aaagctaaaa atgatgaatt agaaaaatta gaaaattctt
     2941 ttaaagaaaa atttgaaaat gaattagatg atgcatattt agctacatta attagtgagc
     3001 tagatgtatc taaattaatt gatatggtaa aagatgaaca aactaaattg cttaatgaaa
     3061 tcaatagatt aaaagctgaa atagaaaatc aattatttca aattttttca aaaaatattg
     3121 atgaagctga ttggttaata gagctaaata attataaaca agaaagaaaa aaagatgata
     3181 acacattaaa atttttagaa ttagaacttg caaaactcga agtagatgtt actgattatt
     3241 acacaaatga cattgatgaa gaatatgatt ctagaaaact actagattta caagaaaaat
     3301 taaataatct taatatttta gaaaaccaac taaaaaatat agaacgtgaa ttaaaaaatg
     3361 aaaatgataa attagaaaaa ttaaaagaga aaattcaaaa tgtattcgat tcattaaata
     3421 tacaaattcc ggagaatgaa tgggaaggta agttaaaaga attaataaaa gaaactacaa
     3481 aaatcaaaaa tgatataaac gccattgctg gtgaattagc aggacttggc atagatcctg
     3541 atgattttat tacagaagat ccaggtatca aatttgatcc caccaaacta ttggatttag
     3601 aaaatttaat aaatgagaaa agtaatcaat taaaagaaat agaaaaagaa aatctcgatt
     3661 ataaaggtga aatagcaaat atcacaggtc tatccatcaa agatgattgg aatatactga
     3721 tagatcactt gtacgaaaaa caaaatgaaa ttttaaatca attaaagcat caagaatcat
     3781 ttattatagc cggtatacta gtacatcaag ccattagcga attgcttcgt gcagaagatg
     3841 aacatataca aaatagcata aactcaccaa atttttctga tttgttattt aatattactg
     3901 ggaaatataa taatttatta atcgatgatg gtggtaagat actgattagt agcgatacag
     3961 cagaatataa gcttcaggat ctcagcactg gcaccaaaga acaagtgctt ttcgcattac
     4021 gtgtgacact tgcagaaaaa attcttgacg acaaagcatt ttttatccta gatgatgcct
     4081 ttcagcacag tgactatgat agacgcccta agctaataga tcaagttttt tcattagcag
     4141 acaaaggttg gcaaattatt tatctgacta tggataaaca tatacaaaaa ttgtttagtg
     4201 ataaagctag taataggcag gattttaatg agattgtgct agatagatta tcataaagag
     4261 gtatttttct ctagaaaaat aatttgtata ttataaatta atattaaatt tgaaaaaatt
     4321 aaaaaatttt aaaaattaat ataatgcatt gaggtatata ttatagcatt aataaaaata
     4381 taaaaaatta tataaaatta aattcataca tttttaaaat tgaaaagaaa atttcaaagt
     4441 aaaaataacc ctactgtttg atataaagga gaaattatga caaatcaaat aaaaaatata
     4501 aaagagaatc acaatctcga aaatactaaa aaattagatt ttttttcgga ccataacaca
     4561 aatgaattaa aagaaaatga gcaactgagt cttcttgaca atgttcaatt tatctacgaa
     4621 ttatccttgg ctcagttaga gttacaatca cttggagttt attttgaaat gaccaatagt
     4681 ttaaggaatt ttaagttaat agaaactcaa aatgatgatg aactactaaa aaaattggca
     4741 tacttcaaat ctgtaaaagg caaccccact tattattacc agattattca aaagaatagg
     4801 acgcgttctg ttaatcaata tctcactcat tggatttatc cttacaaagg aaaatttcac
     4861 ccacaaatga ttagagcact tttaaatatt attggacttg agcagggaga taccgtttta
     4921 gatccatata ttggaagcgg cactacggca ttggaagcac aactcttggg tataaattgt
     4981 ataggcatag atgtttctcc tttatgtgtt attcaaagca aagtaaaaac agaatcgata
     5041 gatgttttct cagaaatatt agaatggaag gatgagataa taaaaaatat agaaccctca
     5101 ttgtttaata tgaatggtaa tactattgat aatactatta atataattcc aaatgaaagg
     5161 gttagaaatt tttataaaat ggcaaaactt gtatctataa gtgataatgc gagaagaggt
     5221 aaggatttta aaactgcatt cgtgaaaaat atagaaatga tgattgcttc tgtttctgat
     5281 tatatcgaaa taaaaaatga tctcaattta aaattaggta atattgatat taaaactgga
     5341 gattctagag cactaccttt agaaaataat agtatcgatg gtattattac ttctccacct
     5401 tattctattg ctttggatta tgtttcaaat gatgcacatg ctttggaaga gttgggatat
     5461 aatctttcag aaattagaga ggaattcata ggtgtgagag gaaaaggacg tacacgaata
     5521 gacctatata atgatgatat gaaaaacagt ttagaagaaa tgtatagagt tctaaagccc
     5581 aacaaatatg cagctatagt tattggaaac gctacttata tggcacaaga agtaaaaact
     5641 gttgaattta caataaatta tgcagaaaaa attggattca aattggttaa aaatatagat
     5701 aaaattattt ttggactgta taatgtaatg caaaaggaaa atattttgat ttttcaaaag
     5761 tagtaataat atgaccaaaa aagaacaaga gaaatatata aaagaattag aggaaactat
     5821 ttctcaattt atcaaaccac ttaaaaatat tcctttcaaa attgttttaa aagcgatatc
     5881 tgagtgcgaa ctcataccat ttgacaaaaa taatcccaaa gataagcaat tattagatga
     5941 tttaattaaa gctgctacca ttgctacaaa taatgcttat gaaaagggta tatttacaag
     6001 aagagaaaat gaagttggga atcatataga gccttttgtg attgatgccc taaatcaaat
     6061 tggtcttgta gcaagtagac ctaaaacaaa agaaggaaag aaaaaagcta caggatatcc
     6121 agatattttc cttgaagata gaaatggaag gccaaattac atagagtgca aaacttataa
     6181 tgaaagaaat tatcaaacaa ctcaaagatc tttttatttc tcacccgcgg aaaggacagc
     6241 tgattttaaa gttattcatg atgcacgaca tctagtcata agttttaaaa ttgaaaaaag
     6301 cgaacgtgat ggtcaaatgg tattcttccc tgtttattgg aaaattttta gtcttgatag
     6361 tttgattggt cagataaagc atgagtttaa ttctactaat aaacaactat atagagatga
     6421 agcattactt gcagaaggta gtatttaatg taaaattctt gacgacaaag cattttttat
     6481 cctagatgat gcctttcagc acagtgacta tgatagacgc cctaagctaa tagatcaagt
     6541 tttttcatta gcagacaaag gttggcaaat tatttatctg actatggata aacatataca
     6601 aaaattgttt agtgataaag ctagtaatag gcaggatttt aatgagattg tgctagatag
     6661 attatcataa agaggtattt tttgcaatag caaattatta ttatttttac atattatttt
     6721 tatacgattt tttttaattt ttatacataa atggaaacaa aagaacaaaa attttaccaa
     6781 gcattacaag atgttttcat cggtgcaaga attgagggac aaggcggttt tgttaatttg
     6841 atgaaaataa aatcgaatta ctatagcaaa atagaaaaat taatcaaaga agatatcgaa
     6901 aaagccttaa tgcaatatcc tgactttagg aatgaacttt tcgataaact ttattctttt
     6961 tttagccgtt actttagcga aagcggttcc atctacttta attctacacc ttttcataat
     7021 aatgtttatg aaaaagtcta tactgatgaa aaagatgtta ttctgttttg gaaaacacat
     7081 atgctttact acgtaaaaac agataggatt ttcaaaagta tggaaataga agtagatggg
     7141 tttaagtttc acttcgatgt atctgagcta gaacacaaaa aatctaatga aaaacgtaat
     7201 ctgatttatg aattaaaaga ggtaagaaaa gacaaaacat tagtttttaa tgttctttat
     7261 tccgaaagag gtaggaaaac gaaacaaaat gagattctta ggacattaaa aaaggaaaaa
     7321 atatcgattg acgaagaagt tttagaaaaa gcatttagaa catttgaaaa gcaaagtgag
     7381 gtagatttct ttatcaataa aaacgctaaa gcctttttgc aagagcaatt taagctctgg
     7441 agttatcaat
//
LOCUS       DAOPRN010000083         7353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_8364_length_7353_cov_125.802549, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000083 DAOPRN010000000
VERSION     DAOPRN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8364_length_7353_cov_125.802549"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PKI65_07720"
     CDS             <1..821
                     /locus_tag="PKI65_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB78357.1"
                     /translation="SFPGGNPSTSTQTNPIVTFNTPGTYTITLTASNGNQNNTYSQLI
                     TVNQNPIININVSPSTNICYGNSVTLTATGATSYQWNNNLGTNNSITITPTSSTSYSV
                     TGYSNGCTASATTYINVFSTPQAPTITIISNNPIILQSSATSGNQWYKDNNPIAGAVY
                     QTHQVLENGIYYAKVTSNGCISDPSNSIQINSLNINNYDHDNITIYPSPAQDIIYIQT
                     DKKILEVRLIDALGRMVANTDTYQLNLDNISNGVYHLVIKFDDDKIVLPVIIQK"
     gene            936..2276
                     /locus_tag="PKI65_07725"
     CDS             936..2276
                     /locus_tag="PKI65_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOB78358.1"
                     /translation="MKRNVVILGAAGRDFHNFNVYFRDNENYNVVAFTAAQIPDIDDR
                     KYPAELAGKLYPDGIPIFSESDLPKLIKELDIHDVVLSYSDLPYEKVMNISAVVNANG
                     ANFMLLGPNDTMLKSVKPVIAVCAVRTGCGKSQTSRRIIEILMKKGLRVVAVRHPMPY
                     GNLIEQKVQRFATVEDLQKHHCTIEEMEEYEPHVVRGNVIYAGVDYEAILRAAENDPD
                     GCDVILWDGGNNDFPFYKPDLMVGIVDPLRPGHEINYYPGEVVARISDIILINKIDSA
                     SLNDINTVRTNIRNINPNAIIIDGASPISVQDPSQIKGKRVLTVEDGPTLTHGEMNLG
                     AATLAAMKFGAAEIVDPRPYIVGKIKETFEKYPHIGTLLPAMGYGEEQIKDLEATINN
                     TPADVVISGTPIDLSRVIKVNKPIVRVDYELQEIGDPTFDDILEEFVKNFNLKK"
     gene            2322..3230
                     /gene="pyrB"
                     /locus_tag="PKI65_07730"
     CDS             2322..3230
                     /gene="pyrB"
                     /locus_tag="PKI65_07730"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOB78359.1"
                     /translation="MKKNLVSINDFTKDEILHVLEMAKKFEENPDRSILNGKLIAVLF
                     YEPSTRTRLSFESAIQRLGGRIVGFSEATNTSIRKGESLGDTIRTINQYTDMIIMRNP
                     YDGSARYASEISTVPFINAGDGSNQHPTQCLLDLYSILQTQNTLDNLNITFVGDLKYG
                     RTVHSLVKAMTDFNTTFHLVSPLELKLPSAVKYYIKKNKLPYYQYEKIEDAIPLSDII
                     YMTRIQQERFVDPVEYEKVKNSYILHANMLTNVKPNLKVLHPLPRVNEIDPSVDSTPY
                     AYYFQQAKNGLYVRQALICYMLGIEV"
     gene            3252..3722
                     /gene="pyrI"
                     /locus_tag="PKI65_07735"
     CDS             3252..3722
                     /gene="pyrI"
                     /locus_tag="PKI65_07735"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOB78360.1"
                     /translation="MKDKYNKKELKVSALENGTVIDHIPQGVVLQVLRALHLENFDDA
                     FYLGSNLDSKKMGKKGIIKVSNKYFEKEDINKIALIAPTATIIEIKEYEIIKKTKVEL
                     PKEVIGLVKCINPNCITNNEQITTKFEVLNDFDDIKFRCYYCEKTMKKENLEFL"
     gene            complement(4001..5263)
                     /gene="nqrF"
                     /locus_tag="PKI65_07740"
     CDS             complement(4001..5263)
                     /gene="nqrF"
                     /locus_tag="PKI65_07740"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOB78361.1"
                     /translation="MIILISLFTNILLSFLVFGTIIILLVVGLLFAKEKLTPKGLVKI
                     TINGEKELTTEPGDNLLMTLADNFIFLPSACGGSGTCGLCKVQVLRGGGSILPTEIGF
                     FNRKQILDNWRLGCQVKVKENIEIKIPETILGVKQWECEVISNKNVATFIKELILKMP
                     NGEILNFNSGEYIQLYVPDNIIVDFAKDIYVEDEYRDEWDKLGMWNLKMINEESTFRA
                     YSMANYPAENDIIMLNVRIATPPWDKNKNKFIDVPPGICSSYIFSLKPGDKVKISGPY
                     GEFHLKDSDKEMIFIGGGAGMAPMRSHIFHLFKTLHTQRKVSFWYGGRSLKELFYIDQ
                     YRLLEAENPNFKFVVALSEPKREDNWNGPIGFIHQVLYDCYLKDHSEPEECEYYICGP
                     PAMLNAVLELLDNLGVPQENIAFDDFGL"
     gene            complement(5277..5894)
                     /gene="nqrE"
                     /locus_tag="PKI65_07745"
     CDS             complement(5277..5894)
                     /gene="nqrE"
                     /locus_tag="PKI65_07745"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOB78362.1"
                     /translation="MKELINIFVRSIFIDNMIFAYFLGMCSYLAVSKTVKTSNGLGIA
                     VVFVLVVTVPINFFINKYLLSPGALSFINPSLADVDLSFLSFIVFIAVIAAIVQLVEM
                     VIEKFLPTLYISLGIFLPLIAVNCAIMGASLFMQERGLQTFNESLSFALGSGIGWWLA
                     IVSIAAIREKLTYSNIPAPLRGNGIAFIITGLMAIAFMSFMGITI"
     gene            complement(5900..6556)
                     /locus_tag="PKI65_07750"
     CDS             complement(5900..6556)
                     /locus_tag="PKI65_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOB78363.1"
                     /translation="MNDKKIRGKSVFSKNNLKILTEPFSGNNPIIVQILGICSALAVT
                     VKLEPAIVMALSVTFVLSFSNLIYSLIRKTVPPRIRIIVQLIIISTLVIFVDQLLKAF
                     VYDISKQLSVFVGLIITNCVIMGRLEAFASNNNPWTSFLDGVGNGIGYGMILIIVAFF
                     RELLGSGTILGYNVIPQALYNLGYVNNGLMILPPMALILIGVIIWVHRSINKELTDKN
                     "
     gene            complement(7036..>7353)
                     /locus_tag="PKI65_07755"
     CDS             complement(7036..>7353)
                     /locus_tag="PKI65_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78364.1"
                     /translation="KKAFKKVRRNVIKHKEIVNSHRQKRGIYSLSTVKKKASEFTNWI
                     YFKFRGVATKYLQNYIMWYVVMGKYLTGKTVSNTGRMLNLASSDRWAWYRYRELVNIT
                     YVN"
BASE COUNT         2726 a         1170 c         1020 g         2437 t
ORIGIN      
        1 ggtctttccc aggtggaaat ccatctacaa gtacacaaac taatccaata gttacattca
       61 atactcctgg tacctacact atcacactta cagcatctaa tggcaaccaa aataatactt
      121 attcacagtt gatcactgta aatcagaatc ctatcataaa tatcaatgtt agtccttcta
      181 caaatatttg ttatggtaat agtgttacat taacagcaac aggtgctaca agttatcagt
      241 ggaataacaa tttagggaca aataattcta taactattac tcctacatct agcacatcat
      301 atagtgtaac tggttattcc aatggttgca ctgctagtgc tacaacatat attaatgtct
      361 tctctactcc acaagcacct actattacta taataagcaa taatccaata atactacaat
      421 ctagcgctac tagcggaaat caatggtaca aagataacaa tcctatagct ggcgctgttt
      481 atcaaactca tcaagtacta gaaaatggaa tatattatgc aaaagtaact tctaatggct
      541 gcatctctga tccttctaat tctattcaaa ttaatagttt aaatataaat aattacgatc
      601 atgataatat cactatttat cctagtccag cacaggatat tatatacata cagactgata
      661 agaaaatttt agaagttcgt ttgatagatg ctttgggtag aatggtagcc aatactgata
      721 cttatcaatt aaatctagat aatattagta atggggttta tcatttagtg ataaaatttg
      781 atgatgataa aatagtttta cctgtaatta ttcaaaaata attaatatat taaggtttaa
      841 taacaaattt taaatgaata ggtaggtata attcttaaaa aaattatata taataaaaaa
      901 aattatttat ctttgcaaaa acaaaaatta tatttatgaa acgaaatgta gtaatcctag
      961 gtgcagcggg acgagatttt cacaatttta atgtttattt tagagacaat gaaaattata
     1021 atgttgtcgc tttcacagct gcgcaaattc ctgatattga tgataggaaa tatccagcag
     1081 aattagcagg taaattgtat ccagatggca ttccgatttt ttctgaatca gatttaccca
     1141 aattaattaa agaattagat attcatgatg tggttttatc atacagtgat ttaccatatg
     1201 agaaagtgat gaatatatct gcagtagtca atgctaatgg tgcaaatttt atgctacttg
     1261 gtccaaatga cactatgctt aaatcagtaa aaccagtaat tgctgtttgt gctgtgcgta
     1321 ctggatgtgg taaaagtcaa acttcacgaa gaataataga aattttaatg aaaaaagggt
     1381 tgcgtgttgt agctgttcgt catcctatgc cttatggtaa tttaatcgaa caaaaagtac
     1441 aacgttttgc cacagtagaa gatttacaga aacatcactg tactattgaa gaaatggaag
     1501 aatatgaacc ccatgtagtt cgtggtaatg taatttatgc tggagtagac tatgaagcta
     1561 tattgcgagc tgctgagaat gaccctgatg gttgtgatgt gatactatgg gacggtggta
     1621 ataatgattt tcccttttat aaacctgatt taatggttgg tatagttgat ccgcttcgtc
     1681 ctggtcacga aatcaattat tatcctggag aagttgtcgc tagaatatca gatataatac
     1741 ttatcaataa gatagactct gcaagcttaa atgacattaa tactgtaaga actaatatta
     1801 gaaatattaa tcctaatgct ataataatag atggagcatc tcctatatct gttcaggatc
     1861 cttcgcaaat aaaaggtaaa cgagttttaa cagtagaaga tggaccaacc ctcacccatg
     1921 gtgaaatgaa tttaggtgct gctactttag ctgctatgaa atttggtgct gctgaaattg
     1981 tagatccacg accatacatc gtcggtaaaa taaaagaaac attcgaaaaa tatcctcata
     2041 tcgggaccct attacctgct atgggttatg gtgaagaaca aataaaagat ctagaagcta
     2101 ctatcaataa tactccagca gatgtagtaa ttagcggtac tcctatagat ttatcgagag
     2161 taatcaaagt caacaaacct atcgttaggg tagattatga acttcaggaa ataggtgatc
     2221 ctaccttcga tgatatactt gaggaatttg tcaaaaattt taatttaaaa aaataatata
     2281 aaaagcggct attagccgct ttttttttaa taaaaaaata tatgaaaaaa aatctggtaa
     2341 gtataaatga cttcactaaa gatgaaattc ttcatgtttt agaaatggca aaaaaatttg
     2401 aagaaaatcc agataggagt atattaaatg gcaaattgat agctgtactt ttttatgagc
     2461 cttcaacacg tacacgtctt agtttcgaat cagctattca gagattaggc ggtagaattg
     2521 taggtttttc agaagctacc aatactagta tacgcaaagg ggaatcttta ggagatacca
     2581 ttagaaccat caatcaatat actgatatga taattatgcg aaatccttat gatggcagtg
     2641 cacgttatgc tagtgaaatt agtaccgtcc catttataaa tgctggtgat ggatctaatc
     2701 agcatccaac tcaatgtctc ttagatttat atagtatttt gcaaactcaa aatactctag
     2761 ataatcttaa tatcaccttt gtaggtgatt tgaaatatgg cagaacagtg catagtcttg
     2821 taaaagctat gaccgatttt aatactactt ttcaccttgt ttctccttta gaactcaaac
     2881 taccaagcgc agttaaatat tatataaaaa agaataaatt accatactat caatatgaaa
     2941 aaatcgaaga tgctattccc ttaagtgata ttatctatat gactcgcata caacaagaac
     3001 gtttcgtcga tcctgtggaa tatgaaaaag ttaagaattc ttatatttta cacgctaata
     3061 tgcttaccaa tgtgaagccc aatttgaaag tgttgcatcc tttgccacgc gtcaatgaga
     3121 ttgatccgtc tgtagactct acaccatatg cttattattt tcaacaggca aaaaacggat
     3181 tgtatgttag acaagcatta atttgttata tgctagggat agaagtttaa tagatataaa
     3241 attaattatt tatgaaagat aaatataata aaaaagaatt aaaagtttca gcattagaaa
     3301 atggtactgt gatagatcat attcctcagg gagtggtttt acaagtatta cgtgctttac
     3361 atttagaaaa tttcgatgat gctttttatt taggttctaa cttagatagt aaaaaaatgg
     3421 ggaaaaaagg aataataaaa gttagtaata aatatttcga aaaagaagat ataaataaaa
     3481 ttgctcttat agcaccgaca gctactatta ttgaaataaa agaatatgaa attattaaaa
     3541 aaactaaagt agaactacca aaagaagtca taggtttagt gaaatgcata aaccctaatt
     3601 gtattactaa taatgagcaa ataactacaa aatttgaagt attaaatgat tttgatgata
     3661 taaagtttcg atgttattac tgtgaaaaaa ctatgaaaaa agaaaattta gaatttcttt
     3721 aaaaaaaatt gtcaccattt cgttattata tctaataatt aacttaataa ctaattgtct
     3781 gcatatagca aataataact ttcaaaatta ctccttccct accctttttt tgcttcacct
     3841 gtctcacaca catacaccct ctcaattata atttatatat taacaaaaaa ttttattatt
     3901 acaatattaa gctttaaatt gtatacaatt agtatttagt tgtcaaataa attatttttt
     3961 aataatattt tatagatttt tcttttcaat aaatctatgc tcacagccca aaatcatcga
     4021 aagctatatt ctcttgaggc acccctaagt tatccaataa ctcaagcact gcattaagca
     4081 tggctggagg tccacaaata taatattcac actcttctgg ttctgaatga tcttttaaat
     4141 aacaatcata caaaacttga tgaatgaaac caattggacc attccaatta tcttctcgtt
     4201 tgggttcaga cagagcaacg acaaatttaa aatttggatt ttctgcttct aaaagtctat
     4261 attgatctat ataaaaaagt tctttcaagg agcgtccacc ataccagaat gacactttgc
     4321 gttgagtatg taaagttttg aaaagatgaa aaatatgact acgcatagga gccatgccag
     4381 ctccaccacc aataaaaatc atttctttat cagaatcttt caaatgaaat tctccataag
     4441 gaccagagat ttttacttta tcacctggtt tcaaagaaaa gatatagcta gagcatatac
     4501 caggaggtac atcaataaat ttatttttat ttttatccca aggtggagta gctatgcgta
     4561 catttaacat aataatatca ttttcagcag gataatttgc catagaataa gccctaaaag
     4621 tactttcttc atttatcatt tttaagttcc acatacctaa cttatcccat tcatctctgt
     4681 actcatcttc gacataaata tcttttgcaa aatcaactat tatgttatca ggtacataca
     4741 actgaatgta ttcaccacta ttgaaattta aaatttctcc atttggcatt tttaatatta
     4801 attctttaat aaatgtagca acatttttat tagaaattac ttcgcattcc cattgcttga
     4861 cacctaaaat agtttctgga atttttattt cgatattttc ctttactttt acttgacatc
     4921 ctaatctcca attatctaaa atttgctttc tattaaaaaa tcctatttca gttggtaaaa
     4981 tactaccgcc acctcttaaa acttgcactt tacataaacc acaagtacca ctgccaccac
     5041 aagcagaagg taaaaatata aaattatcag ctaaagtcat taaaaggtta tctccaggct
     5101 cagtagttaa ttctttttct ccattaatag tgattttcac tagtccttta ggagttaatt
     5161 tttctttagc aaataataaa ccaacaacta ataaaataat aatagtgcca aaaaccaaaa
     5221 aacttaacaa aatattagta aataaagata ttaatattat catagctatt aaatttttaa
     5281 attgttatac ccataaaact cataaaagca atagccatta aacccgtaat gataaaagct
     5341 ataccattac ctctcaaagg agcaggtata ttactgtaag ttaatttttc tctaatagca
     5401 gctatactca ctattgctag ccaccaccca ataccactac caagagcaaa agataaactt
     5461 tcattaaaag tctgtaagcc tctctcttgc ataaataatg aagctcccat aatagcacaa
     5521 ttgactgcta tcaaaggtaa aaaaatacca agagaaatat aaagtgtagg caaaaatttt
     5581 tctataacca tttctactaa ctgtactata gcagctatta ctgctataaa aactataaag
     5641 ctaagaaagc taagatcaac atcagccaat gaaggattaa taaaagataa agctccaggg
     5701 gaaagtaaat atttattaat aaaaaaattg ataggcacag tgactactaa aacaaaaacc
     5761 actgctatac caagtccatt actagttttg acagttttag aaacagctaa atatgaacac
     5821 atacccaaga agtatgcaaa aatcatatta tctataaaaa tactacgaac aaagatatta
     5881 ataagctctt tcataattat taatttttat ctgtcaattc tttatttata ctacgatgca
     5941 cccatattat tacacctatt aaaattaaag ccattggagg taatatcatc aatccattat
     6001 tcacgtaacc aagattataa agagcctgtg gtattacatt ataacccaaa atagttcctg
     6061 atcctagtaa ttctctaaag aatgctacaa taatcaagat cataccatat cctatgccat
     6121 tacctacacc atctaaaaat gaagtccaag gattattatt actcgcaaat gcttctaatc
     6181 tgcccataat aacacaatta gttattatca atccgacaaa caccgataat tgcttgctaa
     6241 tatcataaac aaaagctttt aataactgat caacaaatat aactaaagtg gaaataatta
     6301 ttaattgcac tattatacgt atcctgggag gaacagtttt tcgaattaat gaataaatca
     6361 gattagaaaa acttaaaaca aaagtaactg ataatgccat aactattgca ggctcaagtt
     6421 taacagtaac tgctaaagca ctacaaatac ctaagatctg gactattata ggattattac
     6481 cactaaaagg ctctgtgagt atttttaaat tatttttaga aaatacagat ttgcctctta
     6541 tttttttgtc attcataata ttttatatta taacatttgc aaaaatataa cttttttaaa
     6601 attgtagtaa tttatgattt ttaaaaaatc ttttaaaatg cagatttaat gggtattata
     6661 gcctaaaaat gatatttggc atctttttat tattatatca ctttctaggg atataattaa
     6721 taaaatgcat caacaatgat agagtataac tatcatttat ttaaatttaa agattctaaa
     6781 aaaactgaaa gtttaattct gaaaatccag ggaaataata taattgaatt aattatgcgt
     6841 gaaatttatt tttatttaca atagacaata ataatttagc ttttaaataa atttccaata
     6901 tattataact aaattataat caaaaatgaa acaaacaaat aatattataa tataataaaa
     6961 gtagttagtt gtgtggtttt tcggtaatat gagttttttt taagggttaa gataatcgag
     7021 gatttaatat atgtttcaat taacatatgt tatattcact aactctctat accgatacca
     7081 tgcccacctg tccgaggatg cgaggttgag cattcgccca gtattgctta cagtcttgcc
     7141 tgtgaggtat ttacccatca ctacatacca cataatgtag ttttgcaggt atttggtagc
     7201 tacgccccta aatttgaaat atatccaatt agtgaattca ctagcctttt tcttgacggt
     7261 acttaggcta tatatgcctc gcttttgtct atggctattt actatttctt tgtgcttgat
     7321 tacattcctt ctaacctttt tgaatgcttt ttt
//
LOCUS       DAOPRN010000084         7280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_8461_length_7280_cov_114.414810, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000084 DAOPRN010000000
VERSION     DAOPRN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8461_length_7280_cov_114.414810"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2500)
                     /locus_tag="PKI65_07760"
     CDS             complement(<1..2500)
                     /locus_tag="PKI65_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOB78365.1"
                     /translation="MIDKNLIKKEINHYFEEIKNIYHTGDYTEWTYRTPFENFIKNIN
                     KNYQLIQEPKRTTGLGAPDFKAYNDNRKIGFIETKDLNANLDEILETEQLKKYIDAID
                     NLILTNYLQFILIRNKKPVLNVDLFTLNDLNKSKNSISNEKVEQFAQLISNFFDYQLP
                     TISTAEELAMQLSKKAILLKDSAIEQLKDDLKNSNSPSSIYDFYQGIKELINDINIED
                     CADAYAQTVTYGLFMAKKNYKTTLNRDSAASYIPKSVGIIRRIFSNISGDEFPSNIAW
                     IVDDILDILNVSDLDKILSNIDERGKKDKEPIIYFYEDFLNYYEPERRKQLGVYYTPR
                     PVVNFIVNSVHIILKEYFDKQFGLADDSVNVLDPAAGTGTFFWIAYLVVLNELKKQGL
                     SGMIEDKIKNHLLKHFYGFELLITPYVISHLNLTDLLKKWKYDLLDNERIQIYLTNTL
                     ERAKLPNEIPFFREVTEENKAANKVKFDEKILAIIGNPPYAGISANKGKWIDDLLKKG
                     YTRADGSKDDGYYKVDGKPLGEKNPKWLQDDYVKFIRFAQWKIDQNGEGVVGFITNHS
                     YIDNPTFRGMRQSLLESFDRIYLLNLHGSSRRGSYDDENVFDIQQGVAISIFIKTKKY
                     NDKKVFYADLWGLREDKYKWLDSHTIKNVEWKEINPVSPNYFFVPKNTSLEEKYNKFW
                     KITDIFPLYSTGIVTAKDDFIIDFNKDALHKRIAKLRDKSISDLEIRNIYKLKDTHGW
                     KLSKARKKLIDDAKWDQYFTKIYYRPFDIREIYYNPIMLEGARYDVMQHLLMDNIAIV
                     TVRQVKYGNSWQHCLIADKLVESSFISNKTSEICY"
     gene            complement(2661..3446)
                     /locus_tag="PKI65_07765"
     CDS             complement(2661..3446)
                     /locus_tag="PKI65_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78366.1"
                     /translation="MKKIKLALFIVLIFSASSIYANNRINNLLKKADSAYNAQDFYIA
                     CQIWESIVENGYINDDIYYNLGNASYRMGDLSKAIWFYKKSLLLSRYNVNAQENLNLL
                     VNKGYAQLLPQPMGVTAFFNKFFTAIPYNTLFWISWVVFILIGILILIFIFRKKYSSR
                     GGIFWILVVLSICFIYFISSFIYQNINIYNSKSAIISVPSTNLRSSPDSLSIAVAIVN
                     EGTEVKCLESLGNWEKVLTPSQKTGWLQREYLFMLRNRLPDNK"
     gene            complement(3450..5318)
                     /locus_tag="PKI65_07770"
     CDS             complement(3450..5318)
                     /locus_tag="PKI65_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOB78367.1"
                     /translation="MMKKINLLSCFIFLFISNCIWGQTFIATTEYTSVPLNQSFDVVY
                     ELKGAKADRFIRPDFEPFRVISQQSYSGGGMTVIINNKVVQDGSGSQKWVFTLLPTST
                     GKYTIGPAKVLVGNDWLESNSITINVTPSGSPTTASAPAPPTQSKSNKPDTDRSKLTN
                     PVVDDEIFLKAEVSKSNVYLGEPIILDYYIYTRVDVRQFGINKTPSFKGFWSENLLDN
                     TANVKTTEKVINGKRYIVAHIRSIALYPQEIGKLKVDPLEVEAIIVRHVAHKDPFDLF
                     GDFFKDPFNFDPFNLLGPSVISQPQKITLKSNPVVINVKPLPKGAPESFSNAVGNFKI
                     DAFLDNDRCFAGESIIYNLKISGTGNLPLLSAPELNLPENFQVFEPEIKDAFSKTSKG
                     IAGSRTFKYIITPLKAGNYTIPSVEFSYFSPTLKKYITVNTPELNIQVFDAVGSKGAQ
                     ATIVNLKDDIQYINPIKNLTIFNKLPHNNLFVILMFFIPLLIMIIISIFYRKRLKILT
                     DFTLQKQKRADREARKRLKTAHKYMKENNAKLFFDQLLLALWQFISDKYLIPLSNLNI
                     DSAREVMINHNIDESIIEELLSFIKECQMYAYAPTDTNDNLEKFYKRAYEFIRKKI"
     gene            complement(5318..6052)
                     /locus_tag="PKI65_07775"
     CDS             complement(5318..6052)
                     /locus_tag="PKI65_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB78368.1"
                     /translation="MRAWVLCLFLFLLPSFILSQNEKNIKGESYFYKGNLAYQQGKFQ
                     EAEELYRKGLLLPSDHKYQANFNLANALFKQKKYDLAIKEYQNTLNYVDDGNLIAKSK
                     IYYNIGNVFLEQKQYDKAIEQYIKALKLNPIDSDAIYNLNYAWKMMQQQNQDNQRKDN
                     GKNEDNDNDKNNDKQDQSNNQDQQQDQQQDQQQDQQQDQQSQRSMNKDEIDQLLKAMQ
                     QKELETQKQLRETLNKKSKIVIEKDW"
     gene            complement(6049..7098)
                     /locus_tag="PKI65_07780"
     CDS             complement(6049..7098)
                     /locus_tag="PKI65_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOB78369.1"
                     /translation="MYKFVYPNRALLFIAIPLFLIIFIIFYHKRKKWINTYFSIDNFR
                     QISNNFALNKVITKFIFFLISYVLIVITLMNPLEPKKTVSSTKKEGADVFIVLDISNS
                     MLAEDIKPNRLEKAKQIIKTLLLKFGSDRVGVVLFAGDAFVYLPLTIDLGMASMMISE
                     ADPLLIQPQGTNINKALTTTFYAIERSRVKKPFVIVFSDGENFEEDPHDILKRYADSK
                     IPIYVVALGTPEGAPIPLKQNSDIIGYKRDRNGDLVITRPNIMLLSSIATATNGILID
                     QQNLNRAHEILFDEIKKYQYEKGVEVYIGQYQSVYLWFLIPAFVLLLIDFLMSEHKDK
                     WQFNFQRFINKNIDL"
BASE COUNT         2493 a         1120 c          942 g         2725 t
ORIGIN      
        1 catagcaaat ttcgctagtt ttattagaaa taaagctact ttcgaccaat ttatctgcaa
       61 tcaaacaatg ttgccagcta ttgccatatt ttacttgacg aacagttact atagctatgt
      121 tatccattag taaatgttgc ataacatcat atctagctcc ttctagcatt attggattat
      181 aatatatttc tcttatgtca aaaggtcgat aatatatttt ggtaaaatac tgatcccatt
      241 tagcatcatc aatcaatttt ttccttgctt tcgacaattt ccaaccatga gtatctttta
      301 atttataaat atttctaatt tctaaatcgc ttattgattt atctcttaat tttgctattc
      361 ttttatgtaa agcatcctta ttaaaatcaa tgataaaatc gtcttttgct gttactatac
      421 cagtcgaata caaaggaaaa atatccgtaa tcttccaaaa cttattatat ttctcctcta
      481 atgatgtatt tttaggtaca aaaaaataat ttggagaaac gggatttatt tccttccatt
      541 ctacattttt tattgtatga ctatccaacc atttatattt atcttctctt agcccccaca
      601 ggtctgcata aaacactttt ttgtcattat atttttttgt tttaatgaat atgcttatag
      661 caaccccttg ctgaatatca aaaacatttt catcatcata gcttcctctg cgactacttc
      721 catgtagatt taataaataa attctatcaa aactctctaa aagtgattgt ctcatacctc
      781 tgaatgtagg attgtctata tagctatgat ttgtgataaa acccactact ccttctccat
      841 tttgatctat tttccattga gcaaatctta taaatttaac gtaatcgtct tgaagccact
      901 taggattttt ttcacctaat ggcttaccat ctaccttata atatccatca tccttactac
      961 catcagccct tgtgtaacct ttttttaata aatcatctat ccatttacct ttattagctg
     1021 atatacctgc ataaggagga ttgcctatga ttgctaagat tttttcatca aattttactt
     1081 tatttgcagc tttattttcc tctgttactt ctctaaaaaa tggtatctca ttaggtaact
     1141 ttgcacgttc taatgtatta gttaaataaa tttgtatcct ctcattatcc agaaggtcat
     1201 atttccattt ttttaataaa tctgtaagat ttagatgtga gataacatat ggtgtaatga
     1261 gcaattcgaa accatagaaa tgctttaata gatgattttt tattttatcc tctatcatac
     1321 cactaagtcc ttgtttttta agttcattaa gtactactaa atatgcaatc caaaaaaatg
     1381 taccagtacc ggcagcagga tccaatacat ttacactatc atctgctaat ccaaattgct
     1441 tatcaaaata ttcttttaga ataatatgta ctgaatttac aataaaattg actactgggc
     1501 gaggtgtata atatacgcct aattgctttc ggcgctcagg ctcataataa tttaaaaaat
     1561 cttcgtagaa ataaataatg ggttctttat cttttttacc acgttcatct atattagaca
     1621 gtattttatc aaggtcagaa acattcaata tgtctaatat atcatccact atccaagcga
     1681 tatttgatgg aaattcatca ccagaaatat tagaaaatat tcgcctaatg attccaacac
     1741 ttttgggtat atacgatgct gctgaatctc gatttaatgt agttttgtag ttcttttttg
     1801 ccataaaaag tccataagtt acagtctgag cataggcatc agcacaatct tctatattta
     1861 tatcattgat taactcttta ataccttgat aaaagtcgta aatagatgat ggagaattac
     1921 tattttttag gtcatctttt aattgctcta tagcactatc ttttaataat attgcttttt
     1981 tagatagttg cattgctaat tcctcagcag tagatatggt aggtaactga taatcgaaga
     2041 aattacttat caattgggca aattgctcta ctttttcatt agaaattgaa tttttcgact
     2101 tatttaggtc attaagagta aataaatcta cattcaaaac aggtttttta tttctaatta
     2161 aaataaattg cagataatta gtcaatatca ggttgtcgat agcatcaata tattttttta
     2221 attgctcagt ttctaagatt tcatctagat tggcattcaa atctttggtt tctataaaac
     2281 ctatttttct attatcatta taagccttga aatcaggagc accaagtcca gtagttcttt
     2341 taggctcttg tattagttga taatttttat ttatattttt aataaaattt tcgaaaggtg
     2401 ttctataagt ccattctgta taatcaccag tatggtaaat atttttaatt tcttcgaaat
     2461 aatgatttat ttcttttttt attaaatttt tatctatcat taaaaaaata ttatatagca
     2521 aaaataataa aaaatcagaa ttatgagaga aggactatag tatgtttgat tttttgacaa
     2581 aatataaggg ctattattaa aaaattaatc tactttataa cctgattttt gatattagca
     2641 tagactaata tctgtttcag ttatttatta tcaggtagac gatttctaag cataaataaa
     2701 tattcacgct gaagccaacc agttttttga ctaggagtta aaactttttc ccaatttcca
     2761 agtgattcta gacattttac ttcagtacct tcatttacaa tagctacagc aatagaaaga
     2821 gaatctggac tgctacgcaa attagtagat ggtacagaaa tgatagctga tttagaatta
     2881 taaatattaa tattttgata aataaagcta ctaataaaat aaataaaaca aatagaaagg
     2941 acgactaata tccagaatat accaccacga ctactatatt tttttctaaa aataaaaatt
     3001 agaattaata ttccgattaa gataaatact acccaggaga tccagaataa tgtattataa
     3061 ggtatggcag taaaaaactt attaaaaaag gctgttaccc ccataggttg aggcaaaagt
     3121 tgagcatatc ctttattaac caataaatta agattttcct gcgcatttac attataccgc
     3181 gataacaata gagatttttt ataaaaccaa atagcttttg acaaatctcc cattctataa
     3241 gaagcattac cgaggttata ataaatatca tcattaatat atccattttc tacaatagat
     3301 tcccatattt gacatgcaat atagaagtct tgagcattat aagcactatc agcttttttc
     3361 aacaaattat ttatcctatt attagcataa atagacgaag ctgaaaaaat caaaacaata
     3421 aaaagagcaa gttttatctt tttcatattt caaatctttt ttctaataaa ttcataagca
     3481 cgtttataaa atttttctaa attatcatta gtatcagtag gagcatatgc atacatttga
     3541 cattctttta taaaactaag taactcctct ataatagatt cgtcaatatt atgatttatc
     3601 ataacctcac gtgcagagtc aatatttaga ttagataacg gtatcaaata tttatctgag
     3661 ataaattgcc ataaagctaa aagtaattga tcgaaaaata atttagcatt attttctttc
     3721 atatatttat gagcagtttt tagacgttta cgagcttctc tatcagctct tttttgtttt
     3781 tgcaaagtaa aatctgtaag gatttttaga cgttttctat aaaaaataga aataataatc
     3841 attatcaata aaggaataaa aaacatcaaa atgacaaaca aattattatg aggcaattta
     3901 ttgaaaatag tgagattttt aattggattt atatattgaa tatcatcttt taagttgaca
     3961 atagtagctt gtgcaccttt gctaccaaca gcatcaaaca cttgaatatt taattcaggt
     4021 gtattgacag ttatatattt tttcagtgta ggagagaaat atgagaattc tacagatgga
     4081 atagtgtaat tgcctgcttt gagtggagta atgatatatt taaatgtgcg agatccagcg
     4141 atacctttag aggttttaga aaaagcatct ttgatttctg gctcgaagac ttgaaaattt
     4201 tcgggtaaat tcaattcagg ggcactcagt agtggcaaat ttcccgtacc agaaattttt
     4261 agattataaa ttatactttc acctgcaaag catctatcat tatctaaaaa agcatcaatt
     4321 ttaaaattac ccaccgcatt agaaaaactt tctggagcac ctttgggtaa tggtttaaca
     4381 tttattacta cgggattact tttaagagta attttttgtg gttgactaat gacagaagga
     4441 ccgagtaaat taaaaggatc aaaattaaaa ggatctttaa aaaaatctcc aaataaatca
     4501 aaagggtctt tatgagctac gtgtctcaca ataatagctt ctacctcgag cggatctact
     4561 tttagctttc ctatctcttg tggataaagg gcgatactcc gtatatgagc tactatatat
     4621 cgtttaccat taattacttt ttcggtagtt ttcacattag ctgtattatc gagtaaattt
     4681 tcgctccaaa aacctttaaa agaaggagtt ttattaatgc caaactgtcg tacatctaca
     4741 cgagtataaa tataataatc aaggataatt ggttctccta aatatacatt ggacttacta
     4801 acctctgctt tcaaaaagat ttcatcgtct actacaggat tagttagttt agatctatct
     4861 gtgtcaggtt tgttagattt tgattgtgta ggtggagcag gagctgatgc agtagtaggc
     4921 gatccagatg gcgtaacatt gatagtaata ctattacttt ctagccaatc gttacctact
     4981 aaaactttag caggtccaat agtatactta ccagtactag ttggtaaaag tgtaaaaacc
     5041 catttttgag aaccactacc atcttgtaca actttattat taattatgac cgtcatacca
     5101 ccaccagaat agctctgctg gctaataact ctaaatggtt cgaaatctgg tctaataaac
     5161 ctatcagctt tagcaccttt caattcatat actacatcaa aagattgatt taatggtaca
     5221 gaagtatatt cggtggtagc aataaatgtt tgcccccaaa tacaatttga tataaaaaga
     5281 aatataaagc aagataacaa attaattttt ttcatcatta ccaatctttt tcaataacta
     5341 tttttgattt tttatttaat gtttcgcgta attgtttttg agtttctagt tctttttgct
     5401 gcatagcttt tagtagttga tctatttcgt ctttattcat cgatctttgt gattgttgat
     5461 cttgttgttg atcttgttgt tgatcttgtt gttgatcctg ttgttgatct tgattatttg
     5521 attgatcttg tttgtcatta tttttgtcat tatcattatc ttcattttta ccattatctt
     5581 tacgttgatt gtcttgattt tgttgttgca tcattttcca agcataattt agattgtata
     5641 tagcatcact atcgatagga tttagcttga gagcttttat atattgttct atagctttat
     5701 catattgttt ttgttctaaa aacacatttc cgatattata ataaatttta cttttagcaa
     5761 ttaaatttcc atcatctaca taatttaaag tattttgata ttctttgata gccaaatcat
     5821 attttttttg cttaaataaa gcattagcta gattaaaatt agcttgatat ttgtgatcag
     5881 aaggcaataa taatcctttt ctatatagct cttcagcttc ttgaaattta ccttgctgat
     5941 aagctaaatt tcctttataa aaatacgatt cgccttttat atttttctca ttttgagaca
     6001 atataaaact tggtaataaa aacaaaaata aacataaaac ccatgctctc ataaatcaat
     6061 atttttatta ataaatcttt gaaaattgaa ctgccattta tctttatgtt cagacatcaa
     6121 aaaatctatc aaaagtaaaa caaaagcggg aatcaaaaac cataaataaa cagattgata
     6181 ttgtccaata tatacttcta ctcctttttc atattggtat tttttaattt catcaaataa
     6241 tatttcatga gctctattta ggttttgttg gtcaattagt ataccattgg tagctgtagc
     6301 tatagaacta agcagcatga tatttggcct ggtaattacc aagtcgccat ttctatccct
     6361 tttataacca atgatatcgc tattttgctt taacggtatg ggagcaccct caggagttcc
     6421 taaagcaaca acatatatag gaattttgct atctgcatat ctttttagta tatcatgagg
     6481 gtcttcttcg aaattttctc catcgctaaa cacaataaca aagggttttt ttactctaga
     6541 acgctcgata gcataaaaag tagtagtaag tgctttatta atattagtac cttgaggttg
     6601 tatcaaaagt ggatcagcct cagatatcat catcgaagcc atacctagat ctatagttaa
     6661 tggtaaataa acaaatgcat cacccgcaaa caatacaact cctactctat ctgaaccaaa
     6721 tttgagcaat aatgttttga ttatttgttt ggctttttct aatctattag gctttatatc
     6781 ttcagctaac atagaattac ttatatccaa tacaataaat acatctgctc cttctttttt
     6841 agtagatgag acagtctttt taggttctaa tggattcatc aaagtaataa caatgagtac
     6901 ataagaaatt aaaaaaaata taaatttagt aattacttta tttagagcaa aattattaga
     6961 aatttgtctg aaattatcta tactaaaata ggtattgatc catttttttc ttttatgata
     7021 aaaaataata aaaataatta aaaataatgg tatagctata aacaatagag ctctattagg
     7081 ataaacaaac ttatacatac acctttttta aaaaaaaaaa aacttaatac cgtttttttt
     7141 aatttgtaaa aataaaaatt ttttttaaaa gtattttatt aaaaacgtta ttaagattaa
     7201 attattataa ataaaaagca actacaaatc agaaaaagca aaaatgatac catattaatt
     7261 aatcttaaat tgcaaagtaa
//
LOCUS       DAOPRN010000085         7261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_8496_length_7261_cov_88.740910, whole genome shotgun sequence.
ACCESSION   DAOPRN010000085 DAOPRN010000000
VERSION     DAOPRN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8496_length_7261_cov_88.740910"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /gene="hflX"
                     /locus_tag="PKI65_07785"
     CDS             <1..760
                     /gene="hflX"
                     /locus_tag="PKI65_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOB78370.1"
                     /translation="RLTRMWTHLSKQRGGIGMRGPGEEEIETDRRIIRNKINKLKKQL
                     DEIDTQKQTQRKHRESIVRATLIGYTNVGKSTIMNILSKSNIMVKDKLFSTLDTTTRK
                     VMIKDLSFLLSDTVGFIRKLPTFLIESFKSTLAEVNEADILLHVVDLSSPLYEKQMQV
                     VYQILSEINAVDKTIITIFNKIDLFEEKMLALNPHFDFQSYIDHYKNSYSNNLSVFIS
                     ATKNIGIDELKDLLYDEISKVHEKGFPGNKPFYL"
     gene            952..3876
                     /locus_tag="PKI65_07790"
     CDS             952..3876
                     /locus_tag="PKI65_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOB78371.1"
                     /translation="MNFDLLEKNIKGELYTDELHKIIYATDASVYREIPHGVVYPKDN
                     DDLKIIINFANKNNISLIPRTAGTSLAGQVVGKGLIVDVSRYFNKIIEINPEKKYAIV
                     QPGVILNDLNRKLERYNLFFGPETSTSNRCMLGGMLGNNSCGAHSLIYGSVRDHILAV
                     KVLLSDGSEVFLEELTKDAFFNKCKLNTLEGKIYNHLYDLLSNETNTHLINSQFPHPE
                     VTRRNMGYAIDSLLNTNIFSETSENINLSKLLAGSEGTLAMFTEIKLNLVELPIQEKG
                     LLCVHLDSLSDSFKANLIVLRHNPTSVELIDKVILDLTKENITQQRNRFFVNGDPAAI
                     MVVELWGEDKNIIVEKAKMLKEDLQDHGFGYAFPLILDQDINKVWALRKSGLGVLSNM
                     VGDAKPVAVIEDTAIRPVDLGDFIEELNIELKQLNLSCVYYGHLATGELHLRPILNLK
                     DSKDLKRFRIIAEKTAMLVKKYRGSLSGEHGDGRLRGEFIPTVYGDEIYSMFKSIKQQ
                     WDPHNIFNPGKIVDSPPMDSSLRYVPDAPVLEVDTYYDFSEELGLIRSIEKCNGSADC
                     HKLHWADGGMCPSYQATLDEKNSTRARANALREILNNNISQNPFNNKELYEVLDLCLV
                     CKLCKAECPSNVDMAKFKTEFLQHWYKNHLIPLRSFAIAYISHIQSLFRWIPGIYNFF
                     IKQPLISNLLKSILKIDKNRNFPLMYKFTLKHYLKRNLAALNPTKFKREILLFIDEFS
                     NYNDVRIGIDTIKLLTGLGYSVKILPNKESARTFISKGMLKKAKKIIDKNINLFYENG
                     ASSLPILGIEPSAILGFKDDYPYLCSKELKEKAKILANNAMMIEEFLIKESLNGNISS
                     NDFIPYSGKILVHGHCQQKAIISTKPTIDALKLIPDVDIDEIPSGCCGMAGSFGFEKE
                     HYEISMKIGNLILFPTINSNPNAVIIAPGTSCRQQIYEGTQRQALHPVQFLYKQLKNK
                     IN"
     gene            3989..4636
                     /gene="elbB"
                     /locus_tag="PKI65_07795"
     CDS             3989..4636
                     /gene="elbB"
                     /locus_tag="PKI65_07795"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOB78372.1"
                     /translation="MKKFAIILSGCGVYDGSEIHESTMAMLAIKKHGCDYELFAPEKM
                     QYDVINHLTGQPSDENRNVLVEAARIGRGEVKDLKNLKASDFDILLIPGGFGAAKNLS
                     TYAIDGANMQVDKELERVILEFNNAKKPIGAMCIAPVIIAKVIPGVLVTIGQDLKTAE
                     DIKALGANHKNTNKAEIAIDEKNKVATTPCYMLSSTIADIYDGAYNLISSLLNMV"
     gene            complement(4726..5163)
                     /locus_tag="PKI65_07800"
     CDS             complement(4726..5163)
                     /locus_tag="PKI65_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOB78373.1"
                     /translation="MALLPVSRSESFLPRIIDEFFGDDFLKDFFYMPEQFNVPAVNVV
                     EDENKFQIEVAAPGFKKEDFKVQLDNDVLTIYTEKKEEKEEKDKRYRRREFNYQSFTR
                     SFIVPDYIDKDKIDAEYKDGILFISLPKLKEKIESKGKEIKIK"
     gene            5450..6124
                     /locus_tag="PKI65_07805"
     CDS             5450..6124
                     /locus_tag="PKI65_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78374.1"
                     /translation="MSKRQFPHSYVIVFFIIIIATILTWIIPAGEFDKITILTEQGQR
                     TVIDANSYHKVPQQIQTWQIFSSIFDGIVDKIEIIAFIFIVGASFFLINKGKVLDVAI
                     KKLLHLTRNWREKKIPLIGEGNHLIIVIFMILFSIFGAVFGMSEETIAFIVLFVPMFI
                     ALGYDSIVGVSVVFVGATLGFIGGVTNPFTIGIAQSIAGIPIFSGIEYRLFLWVIFTT
                     IGIIFA"
     gene            complement(6309..7208)
                     /locus_tag="PKI65_07810"
     CDS             complement(6309..7208)
                     /locus_tag="PKI65_07810"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOB78375.1"
                     /translation="MKKLIFISFFIVIYINSGFSQNYSFICGGVTIPDDYPKSTQCEY
                     NSYAFINRYNKVINYIPGNTPVKTIMINFNIMQKDDGSGNFTNSTTDIQRLNNIFNWF
                     SSTYENVASPSDPISGVVSLTDSKIRFELNGIYFYQNTAGWETYSPYTLLNIIENEDP
                     SRLEQLNIFFTEGGSGWAHASLPSINNFDYNSFIVMLGVYNDGNTISDWASAGTLAHE
                     MGHCLDLCHTYLGGGCPNSVADMVIDEEWFDDIFGLPYPGNAPHIADWFADAYDNTIP
                     DGDKITNNVLGGNKAINYFSPNK"
BASE COUNT         2636 a         1016 c         1144 g         2465 t
ORIGIN      
        1 gagacttaca aggatgtgga cacatctctc taaacaacgt ggtggtatag gtatgagagg
       61 ccctggagaa gaagaaatag aaacagatag aaggattatc cgaaacaaaa ttaataaact
      121 aaaaaagcaa ttagatgaga tagatacaca gaaacaaaca cagcgaaaac atagagaatc
      181 cattgttaga gctactttaa taggatatac taacgtaggt aaatctacga ttatgaatat
      241 attaagtaaa tctaatataa tggtaaaaga taaactattt tcgactttag acactactac
      301 gcgtaaagta atgataaagg atttgagctt tttattaagt gatactgtag gttttattcg
      361 taaattgcca acttttttaa tcgaatcttt taaatctacc ttagcagaag tgaatgaagc
      421 tgatatattg cttcatgtag ttgatttatc ttcacctcta tacgaaaaac aaatgcaagt
      481 agtatatcaa atcttatctg aaataaatgc cgtcgataaa actataatta caatttttaa
      541 caaaatcgat ctattcgaag aaaaaatgct agcattaaat cctcattttg attttcaaag
      601 ttatatcgat cattataaaa attcatattc taataattta tcagttttta tctctgctac
      661 aaaaaatatt ggtattgatg aattaaagga tttattatat gatgaaatat caaaagtaca
      721 tgaaaaagga tttccaggta ataaaccttt ttatttgtaa gtatactttt gaaataattt
      781 tataattttt agagaaaatc agtattatat agattgctta atttaatttt tcgatatgca
      841 gaatgcgaga ctgagaaagg atagagaaat gaaatatagc aaattatatc tagcaagcta
      901 attcaaaatt ttttttttaa aatgtatatt taatcttatt tttgtaaaaa aatgaatttt
      961 gatttattag aaaaaaatat aaaaggagag ctatacactg atgagcttca taaaataata
     1021 tatgctacag atgcttcagt ctatcgagag ataccacatg gtgtcgttta tcccaaagac
     1081 aatgatgatt taaaaataat aataaatttt gcaaataaaa ataatataag tctgatccca
     1141 cgaacagcag gaacatctct agcaggtcaa gttgttggca aaggtttgat agtagatgtt
     1201 tctcgatatt ttaataaaat tatagagata aatcctgaaa aaaaatatgc tattgtccaa
     1261 cctggtgtta tattgaacga tctaaataga aaattagaaa gatataattt attttttggc
     1321 cccgagacta gtacaagtaa tagatgcatg ttgggtggta tgttaggaaa taatagttgt
     1381 ggagctcact cactaattta tggttctgta agggatcata tattagctgt caaagtttta
     1441 ttaagtgatg gcagcgaagt atttttagaa gaactaacta aagatgcttt tttcaataaa
     1501 tgcaaattaa atactctaga aggtaaaatt tataatcatt tatatgattt gctttctaat
     1561 gaaactaata ctcacttgat aaactcgcaa tttcctcatc ccgaggttac aaggcgtaat
     1621 atgggttatg ctatagatag cttattaaat actaatatat tctctgagac atcagagaat
     1681 attaatctat caaaattact tgcaggtagc gagggcactc tagctatgtt tactgaaatc
     1741 aaattaaatc tcgtagagct accaatacaa gaaaaaggat tattgtgtgt acatctcgat
     1801 agcttatctg attcattcaa agcaaatctt atagttttgc gacacaatcc cacttctgta
     1861 gagttaatag ataaagtcat attagattta actaaagaaa acataactca acaacgcaat
     1921 agattttttg taaatggtga tccagcagca ataatggtag tagaattatg gggagaagat
     1981 aaaaatatta ttgtcgaaaa agctaaaatg ctaaaagaag acttacaaga tcacggtttt
     2041 ggatatgcct ttccgctaat attagaccaa gatattaata aagtctgggc attgagaaaa
     2101 tctggattgg gtgtattatc taatatggtt ggtgatgcta aacctgtagc tgtgatagaa
     2161 gatactgcta ttagacctgt agatttaggt gattttattg aagaattaaa tatagaactt
     2221 aaacaattaa atttatcttg tgtttactat ggtcacttgg ctacaggtga attacatttg
     2281 agacctatac taaatctaaa agactccaaa gatctcaaac gtttccgaat aatagctgaa
     2341 aaaactgcta tgctagtaaa aaaatatcgc ggttcactaa gtggagagca tggtgatggc
     2401 agattgagag gcgaatttat ccctacagta tatggcgacg agatatattc gatgtttaaa
     2461 tcaataaagc aacaatggga cccgcacaat atcttcaatc ctggcaaaat agttgattcc
     2521 cctcctatgg acagctcgct tagatatgta cctgatgctc ctgtattaga agtagatacg
     2581 tattatgatt tttcagaaga attgggattg ataaggagta tagaaaagtg taatggtagt
     2641 gcagattgtc ataaattaca ttgggctgat ggcggtatgt gtccctcgta tcaagcaact
     2701 ttagatgaga aaaattctac acgcgcgcgt gctaatgcac ttagagaaat attaaataat
     2761 aatataagtc aaaatccttt taataataaa gaattatatg aggtgctaga tctatgtttg
     2821 gtttgcaaat tatgcaaggc tgaatgtccg tctaatgtag atatggcaaa attcaaaact
     2881 gaatttttac aacattggta caaaaatcat ttgataccat tgagaagttt tgctatagca
     2941 tacatttcac atatacaatc attattccgt tggataccag gtatttacaa tttttttata
     3001 aaacaaccat tgatttcaaa tttacttaaa agcatattaa aaatagataa aaaccgtaat
     3061 tttcctttaa tgtacaaatt tactctaaaa cattatttaa aaaggaattt ggcagcactt
     3121 aatcctacaa aatttaagag agaaatttta ttatttattg atgaatttag taattataat
     3181 gacgtaagga taggcataga tactatcaaa ctattgacag gattgggtta ttcagtaaaa
     3241 atattaccaa ataaagaaag cgcaagaaca tttatttcta aaggaatgct aaaaaaggca
     3301 aaaaaaatca ttgacaaaaa cataaactta ttttatgaaa acggagcatc atcattacca
     3361 atattgggca tagaacccag tgctatatta ggatttaaag atgattatcc ttatttatgt
     3421 tcaaaagaat taaaagaaaa agcaaaaata ctggctaata atgctatgat gatagaagaa
     3481 tttttaataa aagaatcttt gaatggaaat atatctagca atgatttcat tccttatagt
     3541 ggtaagatct tagtacatgg tcattgtcag caaaaagcta taatctctac aaagcccaca
     3601 atagatgcct taaaacttat tcccgatgta gatatcgatg agataccttc aggttgttgt
     3661 ggtatggcag gttcttttgg ttttgaaaaa gaacactatg agattagcat gaaaatcgga
     3721 aaccttattt tatttcccac tattaatagc aacccaaatg cagttatcat agctcctggt
     3781 actagttgtc gtcaacaaat atatgaaggg acacaaagac aagccttgca tccagtacaa
     3841 tttttatata aacaactaaa aaataaaatt aattagataa cagctaataa tttgaaaaag
     3901 aggatgatgt ggagataata atatttattt tttttaaaac aatacttgat aaatatcaga
     3961 aaattttata actttgtata aaaaaaatat gaaaaagttt gcaattattt tatcaggatg
     4021 tggagtttat gatggttcag aaattcatga atctactatg gccatgttag caatcaaaaa
     4081 acatggttgt gattatgagt tgtttgcacc agaaaagatg caatatgatg ttataaatca
     4141 tttaacaggt caacctagtg atgaaaatag aaatgtatta gtagaagcag cacgcatagg
     4201 gcgtggagaa gtaaaggatt taaaaaactt aaaagcttca gattttgata ttttactcat
     4261 acctggtggt tttggagcag caaaaaattt aagtacttat gctatagatg gtgcaaatat
     4321 gcaagtagat aaagaattag aaagggtaat attagaattt aataatgcta aaaaacctat
     4381 aggtgctatg tgtatagctc ctgttattat cgctaaagtc ataccaggtg ttttagtaac
     4441 tatagggcaa gacttaaaaa ctgctgaaga tatcaaagca cttggagcta atcacaaaaa
     4501 tactaataaa gctgaaatag ctatcgatga aaaaaataaa gtagctacta ctccttgtta
     4561 tatgcttagc tctactatag ctgatatata tgatggtgct tacaacttga taagttcctt
     4621 attaaatatg gtataaattt attacttttt aaaaaaaagg ctcattaaaa tgagcctttt
     4681 ttttaaaaaa ttgcatactt cttcctgcct gtaaaattgc ttattttact tgatttttat
     4741 ttctttaccc ttgctttcaa ttttttcttt caattttggt aagcttataa acaagatgcc
     4801 atctttatat tcagcatcta ttttgtcttt atctatatag tcgggtacta taaagcttct
     4861 ggtaaagctc tgataattaa attctctgcg acgatagcgt ttgtcttttt cttctttttc
     4921 ttctttcttt tcagtgtaaa tagtcaatac atcattgtct aattgcactt tgaaatcttc
     4981 tttcttaaag ccaggagctg ctacctctat ttgaaatttg ttctcatctt ctacaacatt
     5041 tacagcaggt acattaaatt gctctggcat ataaaagaaa tcttttaaaa aatcatctcc
     5101 aaaaaattca tctattatcc ttggcaggaa gctttcactt ctagatacag gtaataatgc
     5161 catatttttc cctcctttct ttttttattg tttgttttta cttatattag agcaattatt
     5221 ataccaacga ttataatata ttatctatat cgctgataat caaatgatta tagagcattt
     5281 caaatgttaa tcaatgtcaa aatgacacag tttatatcta atttttggtg ataatttgtc
     5341 agaaataaaa tgatgcaaaa acaaagagtg caagaaaacg agaagcagaa aagtaataaa
     5401 aaataaagtt ctctttaagt atcaacttta ttatctttgc taaaaaataa tgtctaaacg
     5461 tcaatttcct catagttatg ttatagtatt ttttattatc attatagcta ctatactgac
     5521 atggataata cctgctggtg aatttgacaa aataacaata ttaactgagc agggacaaag
     5581 aaccgtaata gatgccaata gctatcataa ggtgcctcag cagattcaaa cgtggcaaat
     5641 attttcatct atttttgatg gtatagtaga taaaattgaa attatagcat ttatttttat
     5701 agttggtgct agttttttct taattaataa aggcaaagta ttagatgtgg ctataaaaaa
     5761 attattacac ttgaccagaa attggcgaga aaagaaaata ccactgatcg gagagggtaa
     5821 tcatctgata attgtaattt ttatgatact ttttagcatt tttggtgcag tctttggcat
     5881 gagtgaggag actattgctt ttatagtgct ttttgtacct atgtttatag ccttgggtta
     5941 tgatagcata gtaggagtga gcgtagtatt cgtcggtgcc accttaggat tcattggtgg
     6001 tgttaccaat ccttttacta taggtattgc acagagcatc gcaggtattc ctattttttc
     6061 aggaatagag tatagactgt ttttgtgggt aatattcact actataggta ttatttttgc
     6121 ttgatcgggc attaaaactt tacatgtgat agttaaagtt acaccttgct taattataat
     6181 atctgtatat aattttatat taaaatccca tgtttcattt tcagtaattt caagaggaat
     6241 aggtgaataa ggacaattct taacatatct tctaacagat tttaatgcta atgctctgtg
     6301 cattttccct atttgtttgg agaaaaataa ttaattgctt tgtttcctcc taacacgttg
     6361 tttgttattt tatctccatc agggatagta ttatcatagg catctgcaaa ccaatcagca
     6421 atatgtggtg cattgcctgg atatggtaaa ccaaatatat catcaaacca ttcttcatct
     6481 attaccatgt ctgctacaga attaggacaa ccaccgccta aatatgtatg acataaatca
     6541 agacaatgcc ccatttcatg ggctagggtg ccagcactag cccaatcact aatagtattt
     6601 ccatcattat atacacctaa cataactata aaactattat aatcaaaatt gttaatactt
     6661 ggcaaacttg catgagccca tccagatccc ccttcagtaa aaaaaatgtt taattgttca
     6721 agccttgaag gatcctcatt ctcaataata tttaataaag tatatggact atatgtttcc
     6781 catcctgctg tattttgata gaagtaaatt ccattgagtt caaatcgaat cttgctatct
     6841 gttaaactta ctacaccaga tattggatca gacggagaag caacattttc ataagtacta
     6901 ctaaaccaat taaaaatatt atttaatctt tgtatatcag ttgtactatt agtaaaattt
     6961 ccagaaccat cgtccttttg cattatatta aaattaatca ttattgtttt tacaggtgta
     7021 tttccaggta tataattaat gactttatta tatctattaa taaaagcata tgaattatac
     7081 tcacattgtg ttgattttgg ataatcatca ggaatagtaa ctcctccaca gataaatgaa
     7141 tagttttgac taaaaccact attaatataa attacaataa aaaatgatat aaaaattaat
     7201 tttttcatag gattattatt tttagttaac aattattttt tttatccaat aatttccgtc
     7261 t
//
LOCUS       DAOPRN010000086         7004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_8908_length_7004_cov_99.024176, whole genome shotgun sequence.
ACCESSION   DAOPRN010000086 DAOPRN010000000
VERSION     DAOPRN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8908_length_7004_cov_99.024176"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..884
                     /gene="atpG"
                     /locus_tag="PKI65_07815"
     CDS             18..884
                     /gene="atpG"
                     /locus_tag="PKI65_07815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOB78376.1"
                     /translation="MATLKAIKTRIKSIESTQQITQAMKLVSAAKLRRTQDKIIHLRQ
                     YSKLLTEFFQLVYEEKGNSLTNFFDQRDKGKILIIAFSSNRGLCGSFNANVIREVNNF
                     INNLTDEEKENTEIITIGKKIYDGLKKNNYQIVGRYDELIDKLDYDAIKNLTKLWVQD
                     FIDNKYQKIIFIHNKFKNAAQQFVNIYQFLPLTFKNEVSNNSLYILEPEKEDFIKILL
                     PKYLNTVAYQTFLDSSVSEHGARMTAMSKATENANELLKDLKLEYNKARQDAITKELI
                     EIASGVEALKDQ"
     gene            1040..1420
                     /locus_tag="PKI65_07820"
     CDS             1040..1420
                     /locus_tag="PKI65_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78377.1"
                     /translation="MKSKIFIFLLVVLGLLLSFSSCEKEEGCKAVITVKLLRDTNQVV
                     PYANIRIWQVNSDINVNGQADAKGRFEHLFKNEAILNVTAWSSLFIPFDTLVIDTLAN
                     DTMYGETTIRLKKGETVYKSVFID"
     gene            complement(1417..1998)
                     /locus_tag="PKI65_07825"
     CDS             complement(1417..1998)
                     /locus_tag="PKI65_07825"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOB78378.1"
                     /translation="MFLESKKEKNKAGWIEVIVGSMFSGKTEELIRRLRRAQIANLKV
                     EIYKPAIDKRYNEQQIVSHNSMSLLSTPVDNSQSILLLSSEAEVVGIDEAQFFDDELV
                     DVCNILANKGIRVIVAGLDMNFKGSPFGPMPKLLAIAEYITKVHAICVRCGDIAQYSF
                     RKNANENLVLIGEKDLYEPLCRACYLKAIKNKY"
     gene            complement(2197..3198)
                     /locus_tag="PKI65_07830"
     CDS             complement(2197..3198)
                     /locus_tag="PKI65_07830"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOB78379.1"
                     /translation="MKLAVIGATGLVGNEIIKVLEENKLNISDLYLVASKKSCGKIIT
                     FNNKTYNCITIEDLLNKDIDIAILSAGSKISEIYAPILAKKGTFVIDNSSQWRMHESV
                     PLVVPEVNSNTISENTKIIANPNCSTIQMVLALSRLHWEYKLKQIVVATYQSVTGSGM
                     KGVLQLESERKGEISERIYPHQIDLNCLPHAGDFLDNNYTTEEIKLINETHKIFNDHN
                     IKISPTAVRVPVIGGHSEAIHAVFEKEIDISKVIKLLNDTPGVTVIDNPNLCEYPMPI
                     NVKGKNEVFIGRIRLDLFDKKAINMWVVADNLRKGASTNAVQIAEYIINNIIRKNNL"
     gene            complement(3343..6672)
                     /gene="secA"
                     /locus_tag="PKI65_07835"
     CDS             complement(3343..6672)
                     /gene="secA"
                     /locus_tag="PKI65_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOB78380.1"
                     /translation="MRGILKKIMPTKHERDMKALWPIVDEINEAYKSVEKLSNDELRN
                     KTYEFKKIISETIEKEEKELNELKTRLDEDLEMSIDQKEKIFHQIEELENIIYQHTQK
                     VLNDILPEAFAVVKETTRRFKENSQIVVTATDFDRELATRKSYVKIDGDKAIWSNTWV
                     AGGTEITWDMVPYDVQLIGGVVLHQGKIAEMATGEGKTLVATLPVYLNALPGRGVHIV
                     TVNDYLARRDQEWMGPIYEFLFLTVDCIDKHEPNSEARKKAYLADITYGTNNEFGFDY
                     LRDNMAFSLDDIVQRGHYYAIVDEVDSILIDDARTPLIISGPTTQVREQEYDDLKPYV
                     VKLVNAQRQLINKLLNDAKDYLQKPNRTNEDEKLGGEALLRAHRGLPKYGPLIKFLSE
                     PGIKAILYKIENIYLAENQKNMPIIDSELYFVIDERNNSVDLTDKGIELLAKETGDPK
                     FFIIPDIGSELAELEHSDINPKEKQEKKNELLEEFAVKSDRIHTMQQLLRAYTLFEKD
                     VEYVVLDNKVKIVDEQTGRILEGRRYSDGLHEAIEAKENVKVEAATQTYATITLQNYF
                     RMYKKLAGMTGTAETEAAEFWDIYKLDVVVIPTNKPCVRIDAEDLVFRTKREKYNAVI
                     NEVVKLVEQDRPVLIGTTSIETSELLSKMLNLRKIKHSVLNAKHHQREAEIVAKAGLA
                     GSVTIATNMAGRGTDIKLGPGVREAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGTS
                     QFFVSLEDDLMRLFGSDKMIKVMDRIGYKEGEVIQHPMITKSIERAQKKVEENNFSIR
                     KRLLEYDDVMNTQRTVIYNMRRNALYGEKLDADIVQMFEKVANDSLEFYQDQADFETF
                     SNFCLQTFGIDPPFDEKRFLSDKASTLSKELFNAAYQAYLDKRHYYAEKFYPVIKEIY
                     ENAGKRYENVAIPYSDGKNQFTIIAPLKKSYKTKGLELMKSIEQFVTFSIIDRAWMDH
                     LRVMDDLKQSVNAAVYEQKDPLLIYKIEGAQLFEQLLDKIYIEVVKALSIGRIPIAEE
                     QVQETDQVKSPSKVRNYKTGRDYSTPAGTSQAAPKRQEYNDPSEVAVANEPYRSQKVG
                     RNDPCPCGSGKKYKQCHGRIN"
BASE COUNT         2351 a         1136 c          925 g         2592 t
ORIGIN      
        1 aagaaaaaaa ataagctatg gctacattaa aagctattaa aacacgtatt aaatcaatag
       61 agtccacaca gcagatcact caagctatga aattggtatc tgcagccaaa ctcagacgta
      121 ctcaagataa aatcatacac ttaaggcaat attctaaact attaactgaa ttttttcaat
      181 tagtatatga agaaaaaggt aattcattaa caaatttttt tgaccagaga gataaaggta
      241 aaatacttat aattgctttt tcatctaata ggggattatg cggatctttt aatgcaaatg
      301 ttataagaga agttaataat tttataaata atctaactga tgaagaaaaa gaaaatactg
      361 aaataataac aataggcaaa aaaatttatg atggattaaa gaaaaataat tatcaaattg
      421 tcggcagata tgatgagtta atagacaaat tagactatga tgcgattaaa aatctaacta
      481 aactatgggt acaagatttt attgataata aatatcaaaa aatcattttt atacataata
      541 aatttaaaaa tgccgctcaa cagtttgtaa atatttatca atttctacca ttaactttta
      601 aaaatgaagt gagtaataat agtttgtata ttttagaacc cgaaaaggaa gattttatta
      661 aaattttatt acctaaatat ctaaataccg ttgcatatca aactttttta gattcttcag
      721 ttagtgaaca cggagccaga atgactgcta tgagtaaagc tactgaaaat gctaatgagc
      781 ttttaaaaga tttaaaattg gaatataata aagctcgcca agatgctatt actaaagaat
      841 taattgagat agctagtggt gtagaggcac taaaagatca ataaatattt tattaataaa
      901 ctttaatttt ttcactatat tgactaacaa taatttgtta tatatgactt taaattaata
      961 ttcttataaa attttcaaaa aaaaattgaa aaaaattaat taattaaaaa aattattata
     1021 aatttgaaaa aaaattatta tgaaaagtaa aatttttatt tttcttttgg tagttttagg
     1081 tttattatta tcattttctt cgtgtgaaaa agaagaggga tgtaaagcag taataactgt
     1141 aaaattatta agagatacta atcaagtagt accttatgcg aatataagga tttggcaagt
     1201 aaattcagat ataaatgtaa atggccaagc tgatgctaag ggacgctttg aacatttatt
     1261 taaaaatgaa gcaatattga atgtaactgc ttggtcttct ttatttattc cttttgatac
     1321 gcttgttata gatacattag ctaatgatac tatgtatggc gagacgacca ttagattaaa
     1381 aaaaggtgaa acagtatata aaagtgtatt tattgactaa tatttatttt taattgcttt
     1441 taaataacaa gccctacata aaggttcata taaatctttt tcaccaatta aaaccaaatt
     1501 ttcattagca ttttttctaa atgaatattg agcaatatct ccacatctaa cacaaatagc
     1561 atgtactttt gtaatgtatt ctgcgattgc taaaagtttg ggcataggac caaaagggct
     1621 tcctttaaaa ttcatatcta agccagctac tattaccctt atgcctttat tagctaatat
     1681 attacaaaca tctactaatt catcatcaaa aaattgtgct tcatctatac ctactacctc
     1741 agcttcactg cttaaaagca aaatactctg actattatcg actggagtag ataataagct
     1801 catagaatta tgagatacga tttgttgttc attatatctt ttatctattg ctggtttata
     1861 gatttctacc ttcaaattag ctatctgtgc tctgcgtaat ctacgtatta gctcttcggt
     1921 ttttcccgaa aacatagaac ctacgattac ttctatccac ccagccttat ttttttcttt
     1981 ttttgattct aaaaacataa tacctttatc ttttagcaat aaatattatt ttattaaaag
     2041 ggaaacaaaa tcattattaa acaatcttta atttaataaa caaaaataat tatttttatt
     2101 aaatctaaaa tgattattta acaaaaaaca ttaatatatt taattcaaat aatttaaata
     2161 aattaaatta tttgatgtta atcgttgttt tattgattac agattatttt ttctaataat
     2221 attattaatt atgtattcag ctatctgcac agcatttgta gaagctcctt tccttaaatt
     2281 atcagctact acccacatat taatagcttt tttatcaaat aaatctagac gaatcctacc
     2341 aatgaagact tcatttttac cttttacatt tatcggcatg ggatattcgc ataaattagg
     2401 attatcaata acggtaacac ctggagtatc atttaacaat tttataactt tgctaatatc
     2461 tatttctttt tcgaaaactg catgtatcgc ttcactatga ccacctatta ctggtacacg
     2521 aacagcagta ggtgatattt ttatattatg atcattaaat attttatgag tctcatttat
     2581 aagttttatt tcttcagtag tatagttatt atctaggaaa tcacctgcat gtggcaaaca
     2641 attaagatct atctgatgag gatagatgcg ttcagatatt tcgccctttc tttctgattc
     2701 taattgtaaa acacctttca tgccagagcc agtaacactc tgataggtag ctacaacgat
     2761 ttgttttagt ttgtattccc aatgtaatct cgaaagagct aaaaccattt gaatagtgct
     2821 acaattaggg tttgctatga ttttagtatt ttcagagata gtatttgaat tgacttcagg
     2881 aactactaat ggcacacttt catgcattct ccattgtgag ctattatcta ttacaaaagt
     2941 acctttttta gctaatattg gagcataaat ttcagaaatt ttggatccgg cagataatat
     3001 agctatgtcg atatctttgt ttaataaatc ttctatggtg atacagttat aagttttatt
     3061 attaaatgta ataatttttc cacaagattt tttagatgct actagataaa gatcagaaat
     3121 atttaattta ttttcttcta atacttttat tatctcatta cccaccaaac ctgtagcacc
     3181 tataacagct aatttcattt tttttcacaa aattaaggca aaaatttcaa tttattaagt
     3241 attagtattc agcatattct attacttgat attaacaatt gaaaaatata ataaattaaa
     3301 aaaattgaac aatttttttt aaaatgctta ataccatcta ttttagttaa ttcgtccgtg
     3361 gcattgttta tattttttac cactaccaca aggacaagga tcattacgtc caactttttg
     3421 agatctataa ggttcgttag ctactgctac ttccgaagga tcattgtact cttgacgttt
     3481 aggagctgct tgtgaagtgc ctgctggcgt gctataatcc ctaccggttt tataatttct
     3541 cactttagaa ggacttttta cttgatcagt ttcttgtact tgctcttctg caataggtat
     3601 tcttccaata ctcaatgcct taacgacttc tatataaatt ttatcaagca attgttcaaa
     3661 aagttgtgca ccttcaattt tgtatatcaa aagagggtct ttttgttcat atacagctgc
     3721 gttaacactt tgcttaagat catccataac tcttaaatga tccatccaag ctctatctat
     3781 aatggaaaaa gtaacaaatt gttcgatact tttcattaat tcaagtcctt tagttttata
     3841 agattttttc aagggagcta taatagtaaa ttgatttttg ccatcagaat acggtatagc
     3901 tacattttca tagcgtttac cagcattttc atatatttct tttataacag ggtaaaattt
     3961 ttctgcataa taatgacgtt tatctaaata tgcttgatag gcggcattaa ataattcttt
     4021 agataatgta gaagccttgt cagataaaaa tcttttttcg tcaaatggag ggtctatgcc
     4081 aaaagtttga agacaaaaat tactaaaagt ctcaaaatca gcttgatctt gataaaactc
     4141 tagtgaatca ttagccactt tttcaaacat ttgtacaata tcagcatcga gcttctcgcc
     4201 atatagagca tttcttcgca tattataaat aacagttctc tgagtattca tcacatcatc
     4261 atattcaagt aatcttttac gaatagagaa gttattttct tcaacttttt tctgagctcg
     4321 ttctattgat ttagtaatca tagggtgttg gatgacttcg ccttctttat atccaattct
     4381 atccatcact ttaatcattt tatcactacc aaagagtctc attaagtcat cttctagtga
     4441 aacaaaaaat tgactagtac ctggatcacc ttggcgtccg gaacgtccac gaagttgtct
     4501 atctacacga cgagattcat ggcgttctgt accaatgata gctaacccac cagcttctct
     4561 aacgccaggt cctaatttaa tatcagtacc gcgaccagcc atgtttgtag caatagtaac
     4621 gctaccagca agtcctgctt tagctactat ttcggcttct cgttgatggt gctttgcgtt
     4681 taaaacacta tgttttatct tcctaagatt caacatttta ctaagtaatt cagatgtttc
     4741 tatagatgta gtacctatta atactggtct atcttgctca actaatttta ctacttcatt
     4801 aataacggca ttatattttt ctcttttagt tctaaaaact aaatcttcag catcaattct
     4861 aacacacggt ttgttagtag ggattactac tacatcaagc ttataaatat cccaaaattc
     4921 tgcagcttca gtttctgccg taccagtcat accagcaagt tttttgtaca ttctgaaata
     4981 attttgtaaa gtaatagtag cataagtctg tgtagcggct tctactttaa cattttcttt
     5041 ggcttcaata gcttcgtgca gaccatcaga atatcgacga ccttctaata tacgtcctgt
     5101 ttgttcatcg actattttaa ctttattatc taaaacaaca tattcaacat ctttttcgaa
     5161 aagagtgtaa gctcttagta attgttgcat agtatgaatg cgatcgctct taacggcaaa
     5221 ttcttctaat aattcatttt tcttttcttg tttttcttta ggattaatgt cgctatgttc
     5281 taattcagct aattcactcc caatatcagg aatgataaaa aactttggat caccagtttc
     5341 tttagctagg agctctattc ctttatctgt caaatctaca gaattgtttc tttcatctat
     5401 cacaaaatat aattcagaat caataatagg catgtttttt tgattctctg ctagataaat
     5461 gttttctatt ttatagagaa tagctttaat acctggttca cttaaaaatt taattagagg
     5521 cccatattta ggcaatcctc tatgagcacg tagcagagct tcaccaccaa gtttttcatc
     5581 ttcattagtt ctatttggtt tttgtaaata atctttagca tcattaagta atttatttat
     5641 caattgcctt tgagcattaa ctaattttac aacataaggt ttcaaatcat catattcttg
     5701 ttctctcacc tgagttgtag gaccacttat gatgagagga gtacgagcat catctattaa
     5761 gatagagtct acctcgtcta caatagcata ataatgacct ctctgaacaa tatcatctaa
     5821 agaaaatgcc atattgtcac gtaaatagtc aaaaccaaat tcattattag taccataagt
     5881 aatgtctgcg agatatgctt ttttacgagc ttcactattg ggctcatgtt tatcgatgca
     5941 atctacagtt aaaaaaagaa attcatatat aggtcccatc cattcttggt ctcttctagc
     6001 taggtaatca tttacagtta caatatgcac acctcgtcca ggtaaagcat tcaaatatac
     6061 tggcaaagtg gcaacaagag tttttccttc tccagtagcc atctcagcta ttttaccttg
     6121 atgtaaaacc acaccaccga ttaattgtac atcataaggt accatatccc aagtaatttc
     6181 ggtaccacca gctacccacg tattagacca tatagctttg tctccatcta tttttacata
     6241 actttttctt gtagctaatt ctcgatcaaa atctgttgca gttactacta tttgtgaatt
     6301 ttctttaaaa cgtctagtag tctctttaac aacagcaaaa gcttctggta aaatatcatt
     6361 taatactttt tgtgtgtgtt gataaatgat attttctaat tcttctattt gatgaaatat
     6421 cttttctttt tgatcgattg acatttctaa gtcttcgtct aatcgagttt ttaattcatt
     6481 taattccttt tcttcttttt caatagtttc tgatattatt tttttaaatt cataagtttt
     6541 atttctcaat tcatcattag aaagtttttc tactgattta tatgcttcat ttatttcgtc
     6601 tactataggc cacaaagcct tcatatctcg ctcatgctta gtgggcataa ttttctttaa
     6661 tatacctctc ataattttta tatcccttca atttaaaatt atcttacaaa attacaaaaa
     6721 aattaacaaa taattaagca aaaattaagc caaagaggtt gaatttaaaa gaaaaagatt
     6781 ttaatttttt ttcttattag ttaaaaattt tgaaattggg taataatata gatttgttaa
     6841 gaaaataata aatacatgta tttattattt ttagagaggt gattgagctg acgctgggaa
     6901 ttaacatatg tttcaattaa catatgttat atttactaac tctctatacc gataccacgc
     6961 ccacctgtcc gaagatgcga ggttgagcat tcgcccagta ttgc
//
LOCUS       DAOPRN010000087         6967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_8975_length_6967_cov_101.154948, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000087 DAOPRN010000000
VERSION     DAOPRN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8975_length_6967_cov_101.154948"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1631)
                     /gene="atpA"
                     /locus_tag="PKI65_07840"
     CDS             complement(42..1631)
                     /gene="atpA"
                     /locus_tag="PKI65_07840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOB78381.1"
                     /translation="MSEIRPSEVIALIRQEIAGLDLESNLEEIGTVLQVGDGIARVYG
                     LGGVQSGEMVEFVKSGIKGIVLNLEIDNVGIVVLGDISEIKEGDKVKRLKHIASIMAG
                     EGLLGRVINTLGEPLDGKGPIKGELYEMPLERIAPGVIFREPVNVPLQTGIKAIDTMI
                     PIGRGQRELIIGDRQTGKTAIVIDTIINQRENYEKGEPVYCIYVAIGQKSSTVAQTVK
                     ILEDKGAMPYTVVISAPASDPAAYRLYAPYAGAAIGEYFRDTGRDALVVYDDLSKQAV
                     SYREISLLLRRPPGREAYPGDIFYLHSRLLERAAKIIRNDEVAKQMNDLPESIKDKVK
                     GGGSLTALPIIETQAGDVAAYIPTNVISITDGQIFLETDLFNAGIRPAINVGISVSRV
                     GGNAQLKAMKKVAGTLKIDQAQYRELEAFTKFGSDLDAATMAVLDKGARNVELLKQPQ
                     YKPMPVEKQIAIIFVGTRGLIRNVPINKVRNFEDTYLHKLESLHPEILESLKQGIIND
                     KIESILTELAKEVSKQFEEKK"
     gene            complement(1917..2468)
                     /gene="atpH"
                     /locus_tag="PKI65_07845"
     CDS             complement(1917..2468)
                     /gene="atpH"
                     /locus_tag="PKI65_07845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOB78382.1"
                     /translation="MNVAQRYAKSLLDLAIEQHIAELVYNDLLLINKILNENKDLCIV
                     LQSPIIRNEKKEKIFSEIFAEYIQPLSLNFFTLLLRKSRIVIFKNIVKSYEDFYYEAN
                     NILCVNITTAQHFNESLLDKLQSKIHGFFPDKSLKFNENIDEEILGGFVVNFYDYMID
                     KSIRTKLIQARKQIMRKEYEKKY"
     gene            complement(2475..2966)
                     /gene="atpF"
                     /locus_tag="PKI65_07850"
     CDS             complement(2475..2966)
                     /gene="atpF"
                     /locus_tag="PKI65_07850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOB78383.1"
                     /translation="MLINPSFGLVFWTTVTFILVFLVLKKFAWKPIVNAMKKREETIS
                     NALEQAEKVRQEVAELQAKNEILLQQAKEERDKILAEARQIKDKMIAQAQEQARREAN
                     NIIESAREEIKNEKLRAIEEIRVQIADISLDIAHKVLEHELQNPELSNKIIEEQIEKI
                     TFN"
     gene            complement(3029..3229)
                     /gene="atpE"
                     /locus_tag="PKI65_07855"
     CDS             complement(3029..3229)
                     /gene="atpE"
                     /locus_tag="PKI65_07855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOB78384.1"
                     /translation="MISLSSIGAALTVIGCAYGISTIGKHAMDGIARQPEASNKIQTA
                     MIIACALIEGVSLFGVVVALIK"
     gene            complement(3251..4315)
                     /gene="atpB"
                     /locus_tag="PKI65_07860"
     CDS             complement(3251..4315)
                     /gene="atpB"
                     /locus_tag="PKI65_07860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOB78385.1"
                     /translation="MKIRITLFVFVFSLVTSIYANNHEDTINVNESNTLSVDEIMDAV
                     FDHVLDAYEFHIITIGEKHISLPLPIILIDEGKLVTFSSAKFNHGHTSYLNYKIADKG
                     PKEGRIVKIDKEGNIIENASLIDISITKNAFSLMLSAIILCAILIPIGNKYKKNPDKA
                     PKGFQALMDMVFDFVINGIAKDTLPKNKYWKYVPYLLTLFLFILLNNLMGIIPFFPFG
                     ANASGNIAVTMTLALIALLVINFSGNKNYWREIFANPQAPAWLYPILIPTEIMGMFTK
                     PFALMVRLFANITAGHIIILCIVSVIFIIGSVWMAPVSILLDFFMYFIELLVAFLQAY
                     IFTVLTSLFIGLAMPQHEEN"
     gene            complement(4554..>6967)
                     /locus_tag="PKI65_07865"
     CDS             complement(4554..>6967)
                     /locus_tag="PKI65_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB78386.1"
                     /translation="PGGTTCPGNTLYALLPSLRTNTKQIIEACNTSPTAPTNLTTTLL
                     GCPDNEVKFSWQNSGTGWYIQVSTNSSFPQTSSYIKWISGLTTYTGPTGFVLQSDGAT
                     PLAFQSATTYYWRIYYGGGNYTSTHSFTTLSCSSVPANLSATVLGCPDNEIKFQWQNS
                     GTGWYIQVSTNSSFPQTSSYIKWVSGLTTYTGPAGFVLQSDNATPLGSFLSNTLYYWR
                     IYYGGGNYTTTKTFTTPNCNPPNVTLNATPSSICSGQSATLTASGANTYSWSHSLGSG
                     ASKTVTPTTTTTYTVTGTGSNNATNSALILITVNDCSSTQPTNLTTTLLGCPDNEVQF
                     SWQNSGTGWYIQVSTNSSFPQTSSYIKWVSGLTTYTGPASFVLQSDNITLLDHFQNNT
                     LYYWRIYYGNGNYTDTHSFTTLDCAPMIQVTITADPEIICNGESTTLTASGANTYEWS
                     HNLGTNNTITVYPTTTTTYSVTGTSGNQSNSASIIITVNQIPSINISASPDELCSGNS
                     TTLNVSGANNYNWSHELGNNSTINVYPTSSTTYSVTGTIDGCSSSASVFVNVIQPPIA
                     GSANINTNIICQNNMVTLTLSDFSTNDSIQWQMSNDGINWFDIPSANITPFNYSVTTT
                     GNVYFRAQVSNNCGSVISNILTILSNPIPNTPIITQIDNNPIILQSNAPTGNQWYKNN
                     NTIAGATNQTYLVTENGTYHTVVTINNCSSLPSNQITINNAGINDYANDEILVYPNPA
                     QNIIYIQSSKPINEVMIINNLGQIVAHYSETGIIDINQLADGVYQIMIKTTDNINVLP
                     LLINK"
BASE COUNT         2283 a         1095 c         1063 g         2526 t
ORIGIN      
        1 gatttaatac gtgttttaat agcttttaat gtagccatag cttatttttt ttcttcaaat
       61 tgtttagata cttcctttgc tagctcagtt aatatagatt caattttatc atttataatt
      121 ccttgtttca aggattctag aatttcagga tgtagagatt ctaacttatg caggtaggta
      181 tcttcaaaat ttcttacttt atttattgga acatttctaa ttagtcctct agtgcctaca
      241 aatatgatag ctatttgctt ttctactggc attggcttat attgaggttg ttttagcagt
      301 tctacattgc gagcaccttt atctaatact gccatagtag ctgcatcgag gtcactacca
      361 aatttagtaa atgcttctaa ttctctgtat tgtgcttgat ctattttcaa agtaccagct
      421 acttttttca tagctttcaa ctgagcatta ccgcctacac gacttacaga tatgcctaca
      481 ttgatagcag gacgaatacc agcattgaaa agatcagttt ctaaaaatat ttgaccatca
      541 gtaatagaaa ttacgttggt agggatataa gctgccacat ctcctgcttg ggtttcaatg
      601 ataggtaggg cagttagaga gccaccacct tttactttat ctttaatact ttctggtaaa
      661 tcattcattt gtttggctac ctcatcattt ctaataattt tagcagcacg ttcgagtagt
      721 ctggaatgta gataaaaaat atcaccagga tatgcttctc gtccaggggg tcggcgaagt
      781 agtaaagata tttctcgata tgagactgct tgtttagaca aatcatcata tactaccaga
      841 gcatctctac ctgtatctct aaagtattca cctatcgcag caccagcata aggtgcataa
      901 agtctataag cagctggatc tgaagctgga gcggatatta ctactgtata cggcattgct
      961 cctttatctt caaggatttt tactgtttgg gcaacagtac tacttttttg accaatagca
     1021 acataaatac aataaacagg ttctcctttt tcgtaatttt cacgttgatt tattatggta
     1081 tctattacga tagctgtttt acctgtttgt ctatcaccta ttatcaattc acgttgtcca
     1141 cgaccaatag gtatcatagt atcaatagct ttgataccag tttgcaaagg tacatttaca
     1201 ggttcacgaa agataacacc tggggctatt ctctctaatg gcatttcata taattcacct
     1261 tttataggac ctttgccatc tagtggttcg cctaaggtat ttattactct acctaataaa
     1321 ccttcgcctg ccataattga ggcgatatgt ttgagacgtt ttaccttgtc tccttctttt
     1381 atttcactga tatctcctag taccactata cctacattat ctatttctag attaagtact
     1441 attcctttga tacctgattt tacaaattca accatctcac ctgattgtac gccacctaag
     1501 ccataaaccc gagcaatacc atcgcctact tgcaatacgg tacctatctc ttctaaattg
     1561 ctctcaaggt ctaatccagc aatctcttgg cgaatgagag ctattacttc cgaaggcctt
     1621 atttctgaca taattattat tttttttaat ttacatatca attttaaagt ctaatttata
     1681 aatactaaac attaattaat ctaaatattt taatatttaa aatttacact tttcattaat
     1741 taaattttat ttttaaatta ttatttttta ctaaagaatt aggttatttt aagcataatt
     1801 tattttttgt acgcataaat tttttttctt attttaaaac agtttaacat tctttgttta
     1861 taatttgaga ttgagatatt aataatttta atttacctcg aatttcatat tttactttaa
     1921 tatttctttt catattcttt tctcattatt tgtttgcgag cttgtattaa ttttgttctt
     1981 atacttttat ctatcatata atcataaaaa ttgactacaa atccaccgag tatttcctca
     2041 tcaatatttt cattaaattt caaagattta tctgggaaaa aaccatgaat ttttgattgt
     2101 aatttatcta aaagggattc attaaaatgc tgagccgtag taatattaac acataaaata
     2161 ttattagctt cataatagaa atcctcataa gattttacaa tatttttaaa aataacaatt
     2221 cttgattttc ttagtaataa agtaaaaaaa ttcaaagata aaggttgaat atactctgca
     2281 aatatttctg agaatatttt ttcttttttt tcgtttctga ttattggact ttgcaagaca
     2341 atacacaaat ctttattttc atttaatatc ttattgatta ataaaagatc attatacact
     2401 aactctgcta tgtgttgttc gatagcaaga tctagcagtg atttggcata acgttgagct
     2461 acattcataa ttaatcagtt aaaagttatt ttttctattt gttcttcaat aattttattg
     2521 cttagttctg gattttgtaa ttcatgttca agaaccttat gagcaatatc aagcgaaatg
     2581 tcagctattt gtacacgaat ttcttcgata gcgcgtagtt tttcattttt gatttcttca
     2641 cgagccgatt ctatgatatt attagcttct ctccgagctt gctcttgggc ttgagctatc
     2701 attttatctt taatttgtct agcttcagct aagattttat cgcgttcttc tttagcttgt
     2761 tgcaatagaa tttcattttt tgcttgcaat tcggctactt cttgtcttac tttttctgct
     2821 tgttctaaag cattagaaat tgtttcttca cgttttttca ttgcatttac aattggtttc
     2881 catgcaaatt tttttagcac taagaaaacc aatataaatg taactgtagt ccaaaatact
     2941 aaaccaaaac ttggatttat taacatatca ttcttttttt ttaaaaaaag ctttgcgagg
     3001 tagatttata cctcgcaaag taaatttttt actttatgag ggctactacc actccgaaaa
     3061 gtgatacacc ttctataaga gcacaagcaa taatcattgc agtttggatt ttattagatg
     3121 cttctggttg gcgagctatg ccatccatag catgtttacc aatggttgaa ataccatatg
     3181 cacatcctat tactgtcagt gctgctccga tacttgataa agatatcata attttatatt
     3241 tttggggtta ttaattctct tcatgttgtg gcattgctaa tcctataaat aaagaagtta
     3301 atacagtaaa tatataagct tgcaaaaaag caactaaaag ctcaataaaa tacataaaaa
     3361 aatccaacaa aatagataca ggagccatcc atacagatcc tataataaaa ataacagata
     3421 cgatgcatag aattataata tgcccagcag tgatattagc aaaaagtcgc accataagcg
     3481 caaaaggttt agtaaacatc cccattatct ctgtaggaat caaaatggga tacaaccaag
     3541 ctggtgcttg aggatttgca aatatttctc tccagtaatt tttattaccg ctaaaattaa
     3601 ttactaataa agctatcaat gctaaagtca tagtaacagc tatattacct gaagcattag
     3661 ctccaaaagg gaaaaatggt attataccca tcaaattatt tagtaatata aataaaaata
     3721 atgttagtaa atatggaaca tatttccaat atttattttt tggcaaagtg tctttggcta
     3781 ttccatttat tacaaagtca aataccatgt ccatcaatgc ttgaaatcct ttaggggctt
     3841 tatctggatt ttttttgtat ttattaccaa ttggtattaa tatagcacat aaaatgatag
     3901 cagataacat tagtgaaaat gcattttttg taatagaaat atctattaga gatgcatttt
     3961 ctatgatatt accttcttta tctattttta ctatacgtcc ttccttgggt cctttatcag
     4021 ctattttata atttaaatat gaagtatgtc catgattaaa tttagcagat gaaaaagtga
     4081 ctaatttgcc ttcatcgatc aatataattg gtaatggaag tgatatatgc ttttctccta
     4141 ttgtaattat atgaaattca taagcatcta atacgtgatc aaaaaccgca tccattatct
     4201 cgtctactga tagcgtattg gattcattta catttatagt atcctcatga ttattagcat
     4261 agatgcttgt gacaagggaa aatacaaaca caaaaagagt tatacgaatt ttcattttcc
     4321 acaactttat tgcaaagtaa ataaaaatta tttataaaat gaaaacataa tttgattttt
     4381 tcgaaaaatc aaaaaacaat ctaaattcaa ctaagttttg atttaatgtt aaattaatta
     4441 atctaaatta ttaagtttta ttattgatta gcattgttaa acaaatttat ttaaaaaaca
     4501 aaaagagccc agaataataa ttctaggctc ttttatatta aattcaaaaa aaattactta
     4561 ttaattaata gaggtaaaac attaatatta tccgtagttt ttatcataat ttgataaact
     4621 ccatcagcta attggttgat atcaataatg ccagtttcag aataatgagc tactatctga
     4681 cctaaattat taataatcat aacttcattg ataggtttag aagattgtat atatataata
     4741 ttttgggctg gattaggata tactaatatt tcatcattag cataatcatt tatacctgca
     4801 ttattaatag ttatctgatt agaaggtaaa gatgagcaat tattaatagt aactactgtg
     4861 tgataagtac cattttcagt taccagatat gtttgatttg tcgctcctgc gatagtgtta
     4921 ttatttttat accattgatt accagtagga gcatttgatt gtaatatgat aggattattg
     4981 tcgatctgag ttataatagg tgtattggga ataggattgg ataaaattgt taaaatatta
     5041 cttatgacac taccgcagtt attagaaact tgtgctctaa agtatacatt accagtagta
     5101 gtgacagaat aattaaaagg agtaatgtta gcacttggga tatcaaacca gttaattcca
     5161 tcgttagaca tttgccattg tatagaatca ttagttgaaa aatcagacaa agttaaagta
     5221 accatattat tttgacaaat tatattagta ttaatatttg ctgatccagc aataggtggt
     5281 tgaattacat taacaaatac actagctgaa gaggaacatc cgtcaatagt tccagtaaca
     5341 gaatatgttg ttgatgaagt aggataaaca tttatagttg aattattacc taattcatga
     5401 gaccaattat aattattagc accagataca ttcagagtag ttgaattacc tgagcataat
     5461 tcatcaggag aggctgatat attaatagaa ggtatttgat taacagtaat aataatacta
     5521 gcagaatttg attgatttcc agaagtacct gttactgaat aagtggtagt agttgtaggg
     5581 tatactgtaa tagtattatt tgtacccaaa ttatgagacc attcataagt gttagctcca
     5641 ctagctgtga gtgtagtgct ttcaccgttg caaataattt ctggatctgc agttattgta
     5701 acttgtatca tgggagcaca atctagcgta gtaaaggaat gtgtatctgt ataattacca
     5761 ttgccataat atattctcca ataatataat gtattatttt gaaaatgatc taaaagtgtt
     5821 atgttatccg actgaagaac aaaactagca ggtccagtgt aagtggttaa ccctgagacc
     5881 catttaatat atgaactagt ttgggggaat gacgaattag tagacacttg tatataccag
     5941 ccagtaccag aattttgcca agagaattgt acttcattat ctggacaacc taataatgta
     6001 gtggtcagat tagtaggttg agtactcgaa caatcattga cagtaattaa aattaatgca
     6061 gaattagtag cattatttga tccagtacca gtgactgtgt atgtcgttgt agttgtaggt
     6121 gttacagttt tgctagctcc actacccaga ctgtgtgacc aactgtaagt gtttgctcca
     6181 ctagctgtca atgtagcact ctgtccacta catatagacg atggggtagc atttaatgtt
     6241 acgttaggtg gattacaatt aggagttgta aaggtttttg tagtagtata attacctcca
     6301 ccataatata ttctccaata atataaagta tttgataaaa atgaacctaa tggagtagca
     6361 ttatcagatt gtaagacaaa accagcaggt ccagtatatg tagttaaccc tgagacccat
     6421 ttaatatatg aactagtttg agggaatgac gaattagtag acacttgtat ataccagcca
     6481 gtaccagaat tttgccattg aaattttatt tcattatctg gacatcccaa tactgtagct
     6541 gacaaattag caggtacaga tgaacatgat aaagtagtaa aagagtgagt acttgtataa
     6601 ttacccccac cataatatat tctccaataa tatgttgtag cagattgaaa tgctaaaggt
     6661 gtggcgccgt cagattgtaa gacgaagcct gtaggtccag tataagtagt taaccctgag
     6721 atccatttaa tatatgaact agtttggggg aacgacgaat tagtagatac ttgtatatac
     6781 caaccagtac cagaattttg ccacgaaaat ttcacttcat tatccggaca acctagtaat
     6841 gtagtagtta aatttgtagg agctgttggt gaagtgttac aggcttctat tatttgtttg
     6901 gtatttgtcc ttaatgaagg taataaagca tataatgtat tcccagggca agtagtacca
     6961 ccaggat
//
LOCUS       DAOPRN010000088         6646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_9532_length_6646_cov_103.895464, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000088 DAOPRN010000000
VERSION     DAOPRN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9532_length_6646_cov_103.895464"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..892
                     /locus_tag="PKI65_07870"
     CDS             149..892
                     /locus_tag="PKI65_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOB78387.1"
                     /translation="MITKKEIISIKKLDNKKERYEKGLFIAEGPLLVADLIKRGAKHY
                     KIFTTTKYDFLKNLSSIEYICEKDLLRISSMKTPNNILGLFYIKTQPTIENFIKSDKI
                     LILNDIQDPGNMGNILRTAEWLDFKYIVSTLNSVDIYNPKVVQASMGSIMGINIAYLD
                     LEQIKYISRTHCLIATDIIGESICNFKIDFPYAIIIGNEGSGISKELMNMANIILNIP
                     RKNKLNLPESLNAATAAAIIMWHFTMCIL"
     gene            1211..3568
                     /locus_tag="PKI65_07875"
     CDS             1211..3568
                     /locus_tag="PKI65_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOB78388.1"
                     /translation="MNFYQILFFISFNICTYILVAQNFTISGYVKDANSGEQIIAANV
                     YVTNPLKGTTTNHYGFFSLTLPKGNYTLIISAVGYEKLELDIILDKNYELNIELKPKS
                     YLMEQVVVTAERSDANIHNAEMGVIKMPIEKIKTIPVLFGEVDVIKVIELTPGVLTAG
                     EGSSTYYVRGGGPDQNLILLDEAPVYNANHLMGFFSTFNSDAIQNVELYKSGIPANYG
                     GRISSVLDISMKEGNMRKYQFEGGVGLISSRLAVQGPIKKDTSSFILSARRTYADIVM
                     RPFIKNPLGYYFYDINAKANYIFSPKDRIFISGYLGRDIFDAKVSNDDFSTDIGWGNT
                     TMTVRWNHLFSQKLFSNTTLIYSDYNFNFKAKQEIYNFDLNTAIKDFQLKSDFAYFMN
                     NGWSLKFGIDGIYHTFKPYTASFTMENSPFNLGNPPKQYAGELAAYIHTEGDINKWLR
                     ISAGLRFSYYQQLGPFTRYIFDPITLVNVDSIVYDKNKKVKDYYEAEPRFLARFKIDD
                     KNAIKFSATRNIQFIHMVSPVSITLPTDLWVPSTDSVKPQVGYQLSLGYYHNFLNNSI
                     EMSVEGYYKWFFNQIDYKEESLYENYVLTNIDNNFTYGKGRAYGVEFFLQKKYGTLTG
                     WLGYSYGKTERKFPKINDGKPFPAKYDRTHDVNIVINYKINEAFSISAIWVFATGNTA
                     TVPVSRYFIEGNIVTEYSDRNAYRLPSYHRADLSITWYPQHKKKTRKIEESWNLSIYN
                     IYNRKNPYLIYFDIDGDISQNKITTTAKQLSLFPILPSITWNFKF"
     gene            3581..4450
                     /locus_tag="PKI65_07880"
     CDS             3581..4450
                     /locus_tag="PKI65_07880"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOB78389.1"
                     /translation="MKFNIIYLIILFLFLISCSKDIELNLPEPNKKYVVEGYIENGCH
                     PVIAITRNTSYFDPIDSAYLMNLFVLNAKVYISDGDIEAECHLSMDNLLKGVWPYIYY
                     TTDQIIGEINKIYYIQIIIDSDTITGKDTMPNIVCLDSLWCEPIKDNDTLRELWISLT
                     DPPEKNFYRFFTKRLLRDQAYIPSGLFVLDDVYFSNQYFKVFLMRGFENIYSDSTYRD
                     QEFGKYKIGDTVVVKTANMTRQHYNFWRTAEQEIYIGTSPFINPVRIKHTVDGGLGVF
                     GCYATSYDTIIIY"
     gene            4577..5377
                     /locus_tag="PKI65_07885"
     CDS             4577..5377
                     /locus_tag="PKI65_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOB78390.1"
                     /translation="METRFFDTSQGKLSYSINGSGPVILFIHGFPFNKSIWYPYMEKI
                     SNFTTLAIDLPGHGDSDIYGKPDLDDWSKKIYEFLNFLSINQITIIGHSMGGYVACNF
                     AELFPNNVSGLGLFHSSARADTEEKKQNRDRIIQTIKEDHKKYIYNSVPSLFAKENVL
                     KYKSEIDKLINLSQNMEKQALINAQTAMRDREGHLELLTNANFPFLFIIGKQDPNQPY
                     ETLLAQASLTKISFILMLEHCGHMGFVEDFNRTYWAIESFAKLCYAGV"
     gene            5413..6498
                     /gene="ychF"
                     /locus_tag="PKI65_07890"
     CDS             5413..6498
                     /gene="ychF"
                     /locus_tag="PKI65_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOB78391.1"
                     /translation="MGLKTGLVGISNSGKTTLFNCLSNNKIQTASYSFQNTKSNISIT
                     YVNDPRLDEIHKLISADKKVYATLEIVDIPGLVKGASYGEGIGNTFLNEVRNMDALIY
                     VLRCFEDDLLPHIEGCIDPLRDKDIIDLELQVKDLEQVNRKLQKVEKLVKSGDRSAKD
                     TYNSLLKFKEALENFINIRDLELNEIDKNLIKEFAMLTAKPVFYVLNVDENSIDGNKY
                     TENFINSVSNNVLIVSAKIEEEIMRMENENDRNVFLTEYGLDEPALNKLYKGVYKLLD
                     LITFFTIGGKENRAWNIKNGCTAVEAAKEIHSDIARGFIKAEVISYEDLIQYKSEQAC
                     RAAGKVRLEGKNYVVKDGDILHIRFNV"
BASE COUNT         2427 a          793 c         1067 g         2359 t
ORIGIN      
        1 aaaaatataa aaattattta acatataaac ttgaaaaact taaacattta tagtatttca
       61 atctgttcgt tctttcaata acatttttaa tattgttttt tctatgatat tttttatatt
      121 tgctgttatt atttttaaaa tatatataat gataaccaaa aaggaaatta tatcaataaa
      181 aaaattagat aataaaaaag aaagatatga aaaaggttta tttatcgcag aaggtcctct
      241 tttagtagcc gatttgataa agagaggagc aaaacattat aaaattttta ctactactaa
      301 atatgatttt ttgaagaatt tatcatcaat tgaatatatt tgtgaaaaag atttgttaag
      361 gattagctct atgaaaactc ctaataatat tctaggctta ttttatataa aaacgcaacc
      421 taccattgaa aattttataa aatctgataa aatattaata ctaaacgata tacaagatcc
      481 aggtaatatg ggtaatatac tgcgtacagc tgaatggtta gattttaaat atatagtttc
      541 taccttaaat tctgtagaca tatataatcc taaagtagtc caagcaagta tgggtagtat
      601 tatgggaata aatattgcat atttagatct agaacaaata aaatatattt cacgtactca
      661 ttgtctaata gctactgata tcattggaga gagtatatgt aattttaaaa ttgattttcc
      721 ttatgcaatt atcattggca atgaaggtag tggtatatca aaagagctca tgaacatggc
      781 taatattatt ttaaatattc ctagaaaaaa taaattaaat ttacctgaat cactcaatgc
      841 tgcaacagct gccgccatca ttatgtggca ttttactatg tgtattttat aagttatcca
      901 caattcaaag aattaaaaaa tcctagtatc taacgtatta tcattaaata aaaattataa
      961 tattttgaaa taattgcaga tttatttatt ataactaata ttttagtagt tcaattattt
     1021 ttgataatta gaatgatatc taattactac ttataatgat gtaatgcaaa tctaaaatca
     1081 caagaattta actcacatgt tataaatttt gatttttttt caaatggtta attttttttt
     1141 aatctttatt ttcaaaaata attttattca ttctttatat atatttttgt aataatttat
     1201 tattaaataa atgaattttt atcaaatatt attttttata agttttaata tttgtactta
     1261 tattttagta gctcaaaatt ttacaatctc tggatatgtt aaagatgcta atagtggaga
     1321 gcagataatt gctgctaacg tgtatgtaac taatccattg aaaggtacta ctacaaatca
     1381 ttatggtttt ttttcactca cattgccaaa aggcaattat actcttatta tatcagctgt
     1441 gggttatgaa aaattggaac tagacatcat attagataaa aactatgagc taaatataga
     1501 gttaaagcct aaaagttatc taatggagca agtagtagtt acagctgagc gctcagatgc
     1561 taatatacat aatgctgaga tgggagttat aaaaatgcct atagaaaaga taaaaactat
     1621 tcctgtactc ttcggagaag tagatgtgat aaaagtcata gaactaactc ctggagtttt
     1681 gacagctggt gaaggcagta gtacttatta tgtgcgagga ggaggtcctg atcaaaattt
     1741 gattttatta gatgaggcac cagtttataa tgccaaccac ttgatgggtt ttttttctac
     1801 tttcaattct gatgctatac aaaatgtaga attgtataaa tctggtattc ctgctaatta
     1861 tggggggcgt atatcgtcag ttttagatat ttctatgaaa gaaggtaata tgcggaaata
     1921 tcaatttgaa ggtggggtag gattaatatc tagtagatta gcagtgcagg gaccaattaa
     1981 aaaagatacc tcttctttta tactaagtgc tcgacgcaca tacgctgata tagtaatgag
     2041 accttttatt aaaaatcctt taggttacta tttctatgac attaatgcaa aagctaatta
     2101 tattttttct cccaaagata gaatatttat tagtggttat ttgggtagag atatattcga
     2161 tgcaaaggtg agcaatgatg atttctcaac agatataggc tggggtaata ctactatgac
     2221 tgtgcggtgg aatcatttgt tttctcaaaa attattttct aatactactt taatctatag
     2281 tgattataat tttaatttca aagctaaaca agagatttac aattttgacc tgaatactgc
     2341 tattaaagat tttcaactca aatcagattt tgcttatttt atgaataatg gatggagttt
     2401 aaaatttggt atagatggta tatatcatac tttcaaacct tatactgctt catttactat
     2461 ggaaaacagc ccttttaatc tcggtaaccc accaaaacaa tatgctggcg aacttgccgc
     2521 ttatattcac acagagggtg atataaacaa atggctgcgt atatctgctg gccttagatt
     2581 tagctattat cagcagctag gaccttttac tcgctatata ttcgatccta ttacattggt
     2641 aaatgtagat agtattgtgt atgacaaaaa taaaaaagta aaggattatt atgaagcaga
     2701 accacgtttt ttagcaagat tcaaaataga tgataaaaat gctattaagt tttctgctac
     2761 tcgtaatatt caatttatac atatggtatc acctgtgtcg atcacattgc caactgattt
     2821 atgggtgcct agtacagata gtgtaaaacc tcaagtgggc tatcaattat cattaggata
     2881 ttatcataat tttcttaata attcaataga aatgagtgtc gagggatatt ataaatggtt
     2941 ttttaaccaa attgattata aggaagaatc tctttacgaa aattatgtgc ttacaaatat
     3001 agataataac ttcacctatg gcaaaggtag agcttatgga gttgaatttt ttttacaaaa
     3061 aaagtatggc actttaactg gttggttagg ctatagctat ggtaaaactg agagaaaatt
     3121 tcccaagatc aatgatggca aaccttttcc agctaagtat gatcgtacgc atgatgtgaa
     3181 catagtgatt aattataaga taaatgaagc ttttagtata tcagcaatat gggtatttgc
     3241 tacaggtaat actgctacag tgccagtttc tcgctatttc attgaaggta atattgtaac
     3301 tgaatatagt gataggaatg cttatcgttt accatcatat catagagctg atttgagtat
     3361 cacgtggtat ccacaacata agaaaaaaac aagaaaaata gaagaaagtt ggaatctatc
     3421 tatttacaac atctacaata gaaaaaatcc ttatcttata tattttgata ttgatggtga
     3481 tatatctcaa aacaagatta ctactacagc taagcagtta tcgctttttc ccattttacc
     3541 ttctattaca tggaatttta aattttaata tataattatt atgaaattca atataattta
     3601 cttaattata ttattcttat ttctgatatc ttgttctaag gatatagaac tcaatctgcc
     3661 agaaccaaat aaaaaatatg tagttgaggg atatatagaa aatggttgtc atccagtaat
     3721 agctattaca aggaatacta gttatttcga tcctatagac agtgcatatt tgatgaatct
     3781 ttttgtttta aatgctaaag tatatatttc ggacggagat atagaagccg agtgtcattt
     3841 atctatggat aatcttttga aaggtgtatg gccatacata tattatacta ctgatcaaat
     3901 aataggcgaa ataaataaaa tatattatat acaaattata attgattctg atactattac
     3961 aggaaaagat acaatgccta atattgtgtg tctagactca ctatggtgtg aacctataaa
     4021 agataatgac acgcttagag aattatggat ttcattgaca gatccaccag aaaaaaattt
     4081 ttatcgtttt tttactaaaa ggctactcag agaccaagct tacataccat caggattatt
     4141 tgtattagat gatgtatatt ttagcaatca atattttaaa gtttttctta tgagaggttt
     4201 tgaaaatata tatagtgata gtacatatcg agatcaagaa tttggcaaat acaaaatagg
     4261 tgatactgta gtagtgaaaa ctgctaatat gactcggcag cactataatt tttggaggac
     4321 cgcagaacaa gaaatctaca tcggtactag cccatttata aacccagtgc gaataaagca
     4381 tactgtagat ggaggtttag gtgtatttgg ttgttatgct actagttatg atactattat
     4441 tatttattag tgtttgtaaa aagttctaat ttgttagaaa tcattgaaat gaatttgtta
     4501 tttgcttaaa tataagttgt aaaatttata aatttcattg taatatcgta taaattaatt
     4561 atatttgtga aaaaatatgg aaacaagatt ttttgatact tcacaaggga aattatctta
     4621 tagcattaat ggaagtggtc cagttatact ttttattcat ggttttcctt ttaataaatc
     4681 tatttggtat ccctacatgg aaaaaatatc taattttacc actttggcaa tagatttacc
     4741 aggccatggt gatagtgata tatatggtaa accagattta gatgattggt caaaaaaaat
     4801 ttatgaattt ttaaattttt tatctattaa tcaaataacg attataggtc atagtatggg
     4861 tggttatgtt gcatgtaatt tcgcagagtt atttcctaat aatgtaagcg gattgggact
     4921 atttcatagt tctgctagag cagatactga ggagaaaaag caaaatcgtg atcgtattat
     4981 tcagactatt aaagaagatc ataagaaata tatttataat tctgtaccat ctttatttgc
     5041 taaagaaaat gttttaaaat ataaaagtga aatagataaa ttaattaatt tgtctcaaaa
     5101 tatggaaaaa caagctctta ttaatgctca gaccgctatg cgtgatcgtg aagggcattt
     5161 agaattgcta acaaatgcaa attttccttt tttatttata attggcaagc aagatcctaa
     5221 tcaaccttat gaaactttat tagcccaagc tagtcttact aaaatttctt ttatattaat
     5281 gctcgaacac tgcgggcata tgggatttgt ggaagatttt aatagaactt attgggctat
     5341 cgaaagtttt gcaaagctat gctatgcagg agtataattt tttattttgt tttcttagaa
     5401 taaaataatg ttatgggatt aaaaacaggt cttgtaggta ttagcaatag tggtaaaact
     5461 actttattca attgccttag taataataaa atacaaacag cttcatattc ttttcaaaat
     5521 actaagtcaa atattagcat tacttatgta aatgatcctc gattagatga gatacataaa
     5581 ctaatatctg ctgataaaaa agtttatgca acgttagaaa ttgtagatat acctggatta
     5641 gtaaaaggtg ctagctatgg cgaaggtata ggaaatactt ttttaaatga agtacgcaat
     5701 atggatgcct tgatatacgt attgagatgc tttgaagatg atctattacc ccacatagag
     5761 ggctgtatag atccattaag agataaagat ataatagatt tagaattaca agtaaaagac
     5821 ttagagcaag ttaatcgtaa attacaaaaa gttgaaaaat tagttaaatc tggtgataga
     5881 tcagcaaaag atacttataa tagcttatta aaatttaaag aagcattaga aaattttata
     5941 aatattagag atttagagct taatgaaatt gataaaaact tgattaaaga gtttgctatg
     6001 ctaaccgcta aaccagtatt ttatgtatta aatgtagatg aaaatagtat tgatggaaat
     6061 aaatatactg aaaattttat taactctgtt tctaataatg tattgatagt atcagctaaa
     6121 atagaagaag aaataatgag aatggaaaac gaaaatgata ggaatgtttt tttaactgaa
     6181 tatggactag atgaaccagc tctaaataaa ttatataagg gagtttataa attattggat
     6241 ttaataacat tttttactat tggtggcaaa gaaaatagag cttggaatat taaaaatggt
     6301 tgtacagctg tagaagctgc taaagaaata catagtgata tcgctcgtgg atttattaaa
     6361 gctgaagtga tttcatatga ggatttgata caatataaat cagaacaagc ttgccgtgct
     6421 gcaggtaaag ttagattaga aggcaaaaat tatgttgtta aagatggtga tattttgcat
     6481 attagattta atgtttgatt gcaaatgatc gaatctcgaa tttattaaac aataaagcca
     6541 tggagcgaaa gggtttattc gttttattat tttatattgg gagatggaaa tttactttgc
     6601 aatttaagat taattaatat ggtatcattt ttgctttttc tgattt
//
LOCUS       DAOPRN010000089         6602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_9614_length_6602_cov_118.516878, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000089 DAOPRN010000000
VERSION     DAOPRN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9614_length_6602_cov_118.516878"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PKI65_07895"
     CDS             <1..275
                     /locus_tag="PKI65_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78392.1"
                     /translation="KKVVVNKTQIRRGVYSVQVVDKHIDDFKVWLRRFRGVATKYLQS
                     YLMWFVITHKYLTSEIESDIGRLLNLASSDRWAWDEYWRLVNTKYR"
     gene            678..1484
                     /gene="dapF"
                     /locus_tag="PKI65_07900"
     CDS             678..1484
                     /gene="dapF"
                     /locus_tag="PKI65_07900"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOB78393.1"
                     /translation="MKNFNLPLYKFHGVGNDFVLLDARNIDDSNYIKNTNKICDRHFG
                     IGADGIIFLKNSVKDKSKFSMIYYNYDGTKADMCGNGARCICAFANLLGIVDVSENFI
                     FDTDDGIHRAVIHNRRNYLWDIEISINILSQINKINDDLFFVNTGVPHLVKFVDDIDT
                     IDVYNEGKKYRYSTQYFEKGININFVQYGDNIKIRTYERGVEGETQACGTGVCATAIV
                     INNLFNKSFPISIHTQGGELQINEFIENEKKILTLRGPATLVFITDKYYL"
     gene            1508..2035
                     /locus_tag="PKI65_07905"
     CDS             1508..2035
                     /locus_tag="PKI65_07905"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOB78394.1"
                     /translation="MNLQGKNIRLRAPELDDIDWMYEIENNDCYWYLSETYQPFSKWA
                     IENFIKGQTDIFTEKQYRFVAEDKLKNKIGILDLYNFTPIHKRAFVGIFIDELYRGKH
                     FGLESIKLVENYCFNILQLHQLCALIPSDNVISIHLFEKANFVFQSSRKDWYLIKGKF
                     VDELFYNKWNYNEMD"
     gene            2022..3035
                     /gene="mltG"
                     /locus_tag="PKI65_07910"
     CDS             2022..3035
                     /gene="mltG"
                     /locus_tag="PKI65_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOB78395.1"
                     /translation="MKWIKLFVILIIIISSGFYFTFYFPNVKLSDNKEDTIIYVYPAT
                     SENEFKDMLKQKKLLRLPWTFDLATKIKYFTVKPGRYKIYNGSNNNELINKIRIGDQE
                     PIKLTFNNIRTIEELAEKISGQFIFTKEDFLQIIKDNDFLDSLGFNKQNIACIFLPNT
                     YFFYWTTTPKEFVLRMKKEWDDFWTDERLQKAKKMNLSPEEVCILASIVEAETKKWDE
                     MSIVAGVYINRLKRNMPLESDPTIIFAHQDFEIKRVLNGHLKINSPYNTYKNKGLPPG
                     PILLPFPRTIDSVLNYQQHDYLFFCAKDDLSGYHTFSRTISEHQQCAKKYHTALDRLN
                     IKR"
     gene            3127..6219
                     /locus_tag="PKI65_07915"
     CDS             3127..6219
                     /locus_tag="PKI65_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOB78396.1"
                     /translation="MKKIIAFVTLFLFALVGFSMNIRSIEIQYEPINNLTYKARVICY
                     VYEHGPIDQWISFNWGDGTISYLSLSSVSPSGLPYLKKVIYSGQHTYSGSSTFLLSVS
                     YKCRGPYVFNIPLSVQKSLYTEALLVINPYISDQHSLIFQTPTPFNICSGVLQELDLS
                     IYDPNPNTNAIYTYELLACRDSAGTNINNYNFPPATNDFSLNPNTGLLTWDVPLRGEY
                     SFVVRAKKILSGVIVGYVDRDITIIVNDCQSISPILNTPVDTCIIAGDTLSFPVSASF
                     VLDDTMVLTATGQLIDLGADFLQPIKGINNVASEFNWGSTCDFVSISPYNITFRVDVI
                     NKNNTSSLYNFNNGLLSPFTSNVPVMFNNPCGNGWDNTRYLWFGKNEPAPRALTSPIL
                     DLSNGDYIIEFDMRFEEHTGYGGSNCEGPDEPDEGIYLQYNTTNSDDNWINIVYFNPN
                     PKYQEEGGHEFNLIHWNNYTVLLPNEALTPNTRIRWYQERSTNYDYDHWGLDNIGFLR
                     IADIPSIYKKIDISVIAPAPENLQATATPYGIELKWNKEKCDNAIGYKIYRKASQTSY
                     MPGTCDTGLPDSLGFILINTTTSLNDTTYIDYINFNNLTSGNEYCYRVYAYFSDGARS
                     KVSNETCTIMNDVSPIITHVSISNTDEINGELYLQWSKPDDLDTIAHPGPYKYDIYYG
                     ENLIGDDYIYAISKYGLDDTTFVHQNIDTKNKPYHYQIRLFNKVNNQWHHINSSSVAS
                     SVLLTATPGDKKINLKFEYQVPWSNDSFYVYRYNDFSYSYELIAKTLIPEYIDTGLIN
                     GKTYCYKVLAYGHYSAPGFVTPLENWSQELCAIPIDTEPPCTPILSVNPDCNSLSNIL
                     TWQNVITQCNVQDFSHYEIFYSPTIHGGFYLLSSTIDTTFIHTLTNTIAGCYYVVAID
                     TNNNVSMPSENICSDINNCDLYSLPNVFTPDGDGYNDLFKPFPYDFVEKVHLLIYNRW
                     GNLVFETNDPDINWDGKNINSGKPVPDGVYFYLCEVYEWRLEGLTSRLLTGTITIIRH
                     "
BASE COUNT         2393 a          845 c         1011 g         2353 t
ORIGIN      
        1 gtaaaaaagt tgttgtaaac aaaacgcaga ttagacgcgg tgtatacagt gtacaagtag
       61 tcgataagca tatagatgat ttcaaggttt ggttgcgacg atttagagga gtggctacca
      121 aatacttaca gagctatctg atgtggtttg taatcacaca taaatacttg acaagtgaga
      181 tagaatctga cattggtagg cttttaaatc ttgcatcctc ggacagatgg gcgtgggatg
      241 agtattggcg gctggtgaat acaaagtata gataaactac ttcaagtaaa acaatgattt
      301 gctttgatgt acaaacaatt catttagaca ttgtaaccaa atctattccc ctcactctct
      361 aaactaattt atgttgatta tattaattga tttcataaat aaaattaagt atttaagtac
      421 ttaatgaaaa taaaaagata taaatgaaac gagataatat ataagacatt ggaaaaattg
      481 acatcaagaa attttgaatt ttttagccat aagctacaaa ttccaaaaat agtttgtcca
      541 ttttaatttt gcatgattta tttttgattt taatctaagt taattattac tacaaaaaaa
      601 tattaaaatt atctattttt tatgattgta aaaaaataaa taattttgtt tattataaat
      661 ttgttaaatt ttatttaatg aaaaatttta atttgccctt atacaaattt catggtgttg
      721 gtaatgattt tgttttatta gatgcacgca acatcgatga tagtaattat atcaaaaata
      781 caaataaaat ttgtgatagg cattttggta taggtgctga tggtataatt tttttgaaaa
      841 atagtgtaaa agataaaagt aaattttcaa tgatttatta caattatgat ggcactaaag
      901 ctgatatgtg tggtaatggg gctcgctgta tttgtgcttt tgctaattta ttaggaattg
      961 tagatgtatc cgaaaatttt atatttgaca ccgatgatgg tatacataga gcagtgatac
     1021 acaataggcg aaattatttg tgggatattg aaatttctat taatatttta tcacaaatta
     1081 ataaaataaa tgatgatttg ttttttgtaa atactggggt cccacattta gtaaaatttg
     1141 tggatgatat cgatactata gatgtgtata atgaaggtaa aaaatataga tattcaactc
     1201 aatattttga aaaaggtata aatataaatt ttgtgcagta tggtgataat attaaaatca
     1261 gaacttatga aagaggtgta gaaggagaaa ctcaagcttg tggtactggt gtatgtgcta
     1321 cggctattgt gattaataat ctttttaata aatcatttcc tatttctatt catacgcaag
     1381 gtggtgaatt acaaattaat gaatttatcg aaaatgaaaa aaaaatatta actctacgtg
     1441 gaccagctac attggttttt attactgata aatattattt ataactttgc atagataatt
     1501 tattaatatg aatttacaag ggaaaaatat tagactaaga gctccagaat tagatgacat
     1561 tgattggatg tacgaaatag aaaataatga ctgctattgg taccttagcg aaacatacca
     1621 gccattttct aaatgggcta ttgaaaattt tattaaaggg caaactgata tttttactga
     1681 aaaacaatat agatttgtag ccgaagataa attaaaaaac aaaattggaa tattagattt
     1741 atataatttc acacctattc ataaaagggc ttttgtcggt atatttatag atgaactcta
     1801 ccgaggtaaa catttcggtt tagaatccat aaaacttgta gagaattatt gttttaatat
     1861 tttgcaactt catcaactct gcgctctaat accttctgac aatgtaataa gcatacattt
     1921 atttgaaaaa gctaattttg tatttcaaag ctctagaaaa gattggtatc tgattaaagg
     1981 taaatttgtt gacgaattat tttataataa atggaattat aatgaaatgg attaaacttt
     2041 ttgttatttt aataataatc atatcaagtg gtttttattt cactttttat tttcctaatg
     2101 ttaaattatc tgataataag gaagatacta ttatttacgt ttatcctgca acgagcgaaa
     2161 atgaatttaa agatatgttg aaacaaaaaa aattattgag actaccatgg acttttgact
     2221 tagccactaa aatcaaatat tttactgtaa agcctggtag atataaaata tataatggct
     2281 cgaataataa tgaattaatt aataaaatac gtatcggcga tcaggaacct ataaaattga
     2341 cctttaataa tattagaaca atagaagaat tagctgaaaa aatatctgga caatttattt
     2401 tcacaaagga agattttttg caaattataa aagataatga ttttttagat tcacttggat
     2461 ttaataaaca aaacatagct tgtatatttt tgccaaacac atattttttt tattggacta
     2521 ctacacctaa agaatttgtt ttgagaatga aaaaagaatg ggatgatttc tggacagatg
     2581 agagattgca aaaagcaaaa aaaatgaatc tatcaccaga agaggtatgt atcttagctt
     2641 ctatagtgga agctgaaact aaaaaatggg acgaaatgtc cattgtagca ggtgtttata
     2701 ttaatagatt aaaaagaaat atgcctttag aatctgatcc tactataatt tttgcacatc
     2761 aagattttga aattaaaaga gtattaaatg gacacttgaa aattaattct ccatataata
     2821 cttacaaaaa taaaggatta ccgcctggcc ctatactttt accatttcca cgtacaatag
     2881 attctgtact taattatcaa caacacgatt atttattttt ctgtgctaaa gatgatttat
     2941 caggatatca tacatttagc cgcactatat ctgaacatca acaatgtgct aaaaaatatc
     3001 atacagcctt agacagatta aatataaaaa ggtaaatcaa tcagacttat tatcctatca
     3061 ataaattata aataatgagt ttaataagaa aaatattctt aattttacaa tcaagatttt
     3121 tttaatatga agaaaatcat tgcttttgtt acattatttt tatttgcact tgtaggtttt
     3181 tctatgaata ttagatctat tgaaatacag tatgagccaa ttaacaattt aacatataaa
     3241 gctagagtga tttgttatgt ttatgagcat ggtcccatag atcaatggat ttcatttaac
     3301 tggggtgatg gtactattag ttatttaagt ttatcctctg tatctccatc tggtttacca
     3361 tatctgaaaa aagtaattta cagtggtcaa catacatata gtggatcatc tactttttta
     3421 ttaagtgttt cttataaatg tagaggtcct tatgtattta atatacctct ttcggttcag
     3481 aaatctttgt atacagaagc tttgttagta attaatcctt atatatcaga tcagcattca
     3541 ttgatatttc aaacccctac tccttttaat atttgtagtg gagttttgca agagctggac
     3601 ctcagtatct atgacccaaa tccaaatact aatgctatat atacttatga attgctagct
     3661 tgcagagatt cagctggtac taatatcaat aattataatt ttccccctgc tactaatgat
     3721 ttttctctta atccaaatac aggactactt acttgggatg ttcctttaag aggtgaatac
     3781 agtttcgtag tgagagctaa aaaaatttta tctggagtaa tagtaggtta cgtagataga
     3841 gacattacta ttatagtaaa cgattgtcaa agtattagtc ctattttaaa tacacccgta
     3901 gacacttgca ttatagcagg agacacttta tctttccccg tttcggcttc gtttgtttta
     3961 gatgatacta tggttttaac agctactggt caattaatag acctaggagc tgatttttta
     4021 caacctatca aaggtattaa taatgtagct tcagaattta attggggtag cacctgcgat
     4081 tttgtatcaa ttagccctta taatattaca tttagagtgg atgtaattaa taaaaataat
     4141 acatctagtc tatataattt taataatgga cttcttagtc catttacttc taatgtacca
     4201 gtaatgttta ataatccatg tggtaatgga tgggacaata ctagatattt gtggtttggc
     4261 aaaaatgagc ctgctccacg tgcactcact agtcctatat tagatttatc caatggagat
     4321 tatataatag aatttgatat gagatttgaa gaacatactg gctatggtgg ttctaattgc
     4381 gaaggtcctg atgaaccaga cgagggtatt tatttacaat ataacactac aaattctgat
     4441 gataattgga taaatatagt ttactttaat ccaaatccta aatatcaaga agaagggggg
     4501 catgaattta atttaattca ttggaataat tacacagttt tattacctaa cgaagctctt
     4561 actcctaata cacgaattag atggtatcaa gaacgctcta ctaattatga ttatgatcat
     4621 tggggtttag ataatatcgg ctttttgcgt atagctgata ttcctagtat atacaaaaaa
     4681 atagatattt cagttatagc tccagccccg gaaaatcttc aagctaccgc aaccccttat
     4741 ggaatagagc taaaatggaa taaagaaaaa tgtgataatg ctattggata taaaatttat
     4801 agaaaagctt ctcaaactag ttatatgcca ggtacttgcg atacaggatt accagattca
     4861 cttggtttta ttttgataaa tacaacaact tctttaaatg atactaccta catagattat
     4921 ataaatttta ataatttaac tagtggtaat gaatattgct atcgagtata tgcatacttc
     4981 tctgatggtg ctagaagcaa agtatctaat gagacttgta ctattatgaa tgatgttagc
     5041 cctataatta cacatgtcag catatcaaat acagacgaaa ttaatggaga actctatttg
     5101 caatggtcta aacctgatga tttagatact attgctcatc ctggtcccta caaatatgat
     5161 atatattatg gtgaaaattt aataggagat gattatatat atgcaattag taaatatgga
     5221 ttggatgata caacatttgt ccatcaaaat attgatacta aaaataagcc atatcattat
     5281 caaattagat tatttaataa ggtaaataat cagtggcatc atattaatag ctcatcagta
     5341 gcatcttcgg tattactcac tgctactcct ggagataaga aaattaattt aaaatttgaa
     5401 tatcaagtcc cttggagtaa tgatagcttt tatgtatata gatataacga tttttcttat
     5461 tcatatgagt taattgctaa aactctaata cctgaatata tagatactgg cctaataaat
     5521 ggtaaaactt attgctataa ggtattagct tatggtcatt attctgctcc tggatttgta
     5581 actcccttag aaaattggtc tcaagagcta tgtgctattc ctatagacac agaaccacca
     5641 tgcacaccaa tcctatctgt taatcctgat tgtaattcac tatcaaatat tttaacttgg
     5701 caaaatgtaa ttacacaatg taacgtacaa gattttagtc attatgaaat attttattca
     5761 cccactatac atggtgggtt ttatttgctt tccagtacaa tagatactac atttattcac
     5821 actttgacta atacaatagc tggctgctat tatgtagtag ccatagatac taataataat
     5881 gtatctatgc catcagagaa tatatgttct gatattaata attgcgattt atattcactg
     5941 cctaatgtat tcacacctga tggtgatggt tataatgatt tatttaagcc ttttccttat
     6001 gattttgtag aaaaagtaca tcttttaatt tataatagat gggggaattt agtttttgaa
     6061 actaatgatc ctgatattaa ttgggacggc aaaaatatta attctggtaa acctgttcct
     6121 gatggcgtat atttttattt atgtgaagtc tacgaatgga gactagaagg attgacttca
     6181 agacttttga caggtactat tactattatt aggcattaac aataaaacag tgataaatat
     6241 taaattgtag gagttaaata tttttgacca cacaaaaata tttttttata taaattgaat
     6301 ctcctgcact atgtgaaatt tttttaaaat atttagtcat ttctttttgt aataaatcta
     6361 ataaaaaaag gttctactga aatatagaaa tactaaaatt tatatgattt gtttcgaaat
     6421 catacaagta gatttttaaa aaaataaaga gtttagattt agttaattta atgatttaca
     6481 cattatgtga aaatgtaaaa atttattaaa acctcattat aagctaaata tacgaaagat
     6541 gtaaataata attgatttgt aaaaagtaat taaatggtaa agtttttaat ctatgtttca
     6601 at
//
LOCUS       DAOPRN010000090         6004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_10772_length_6004_cov_95.848210, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000090 DAOPRN010000000
VERSION     DAOPRN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10772_length_6004_cov_95.848210"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..2531
                     /gene="cas3"
                     /locus_tag="PKI65_07920"
     CDS             63..2531
                     /gene="cas3"
                     /locus_tag="PKI65_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOB78397.1"
                     /translation="MNSYTDFYKKITGFTPYEYQKNVAEILLNSDKNIILSVPTGAGK
                     TWASIMPYLYARQCNYENFPQKLIYSLPLRTLTNSIYNDINEILNKDEIKGNYIFNNL
                     TSIQTGEYSEDVYFEKDMIFATIDQTLSNFLCFPLSLSQRQANINAGALIGSYLIFDE
                     FHLLDPQLSMSTLIGIIKMLEGISRFCLMTATLTDKFLNFFNENLNFEIVSLENFPKD
                     KENIKSIIFNPNKNNTLNEQIHKKEIQVVNNTINVQNILREHKNKTIVICNRIETAQN
                     LYLDLKKQKNDSTTLICIHSRYFDSDRQNKEKLIKEYFSKNSEKQDVILVSTQVLEAG
                     MDISCDTMHTEISPINSFIQRAGRCARYENECGKIFVYNILDLSEKELVNVDSVNDID
                     KTEIKKLTIKYLPYDKETCEISFKELQQYDHLDESITKTIINDVLSKTEQNIIENIHA
                     KSYSKEKIRKSWQDCTKNHYSETIRDIQSIDVVLLNIDNFKNKKIQPFIYETISVFRW
                     SLIGWIKKIEQSIEEDEWIIAKAEQGLQSQFDFDWQDKESYFLRKLNTNDLKNYYDTV
                     FISNKYFDYNDAGLIIQNNNNNNESPIKEYEKKEKGSIIFKKDTFYQHTLGLLNCYNQ
                     VFKPNLKYTINRLKKICPNINLELIIKLVLIFHDYGKLNKSWQEPMQKFQSKKTGIPC
                     NEVLAHTDYDELTDKELAQNCGLNKKPSHAGIGAIQLYNMIFDLYNENIARASSSAIL
                     RHHSPLSDSFESFDIPEKYLIDIKNLINEIGIECEYIKKANGEDLKEILPNNDLEQIF
                     YLFLVRILRLCDQKVAESLNKYMI"
     gene            2528..4297
                     /locus_tag="PKI65_07925"
     CDS             2528..4297
                     /locus_tag="PKI65_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78398.1"
                     /translation="MNTFIVNKISGTYSDALEAYGLANLLNKIFDNRDIILEKIIIED
                     KGLYFQITISPDITSEDIDKLSFFSLFPFIKQNNDIDISIYPDYFDYIKQKELKKEKQ
                     ELINKIYNEYKDKNEREKKLKEIDNIYQTIKYIDPSLDVFQQFASYNNYITYEKLYLN
                     FYNNKDIFNKLIKEILQYYSDKNYTDKTLKEVINNRDFNIKITAIQLLNPSKGKGIKS
                     AKANNASNTNFNNSNWIEETMKIIGSLSDMVCQLVKVGTNSYDLKVFVPSYKKVSYSF
                     KQKIIPDFKNHLKGNTPIKIDILNILILIKIAIQHLGNSIIKQKVNNIIDGLYSVYQK
                     DLGQGKAVSNISYLQIPNFIYISNKIENDEWVDILDEQINIIGSIDEQGETIQGLMLY
                     RNFISTSSIYDFLNFSYWYATYLSSKLSKNEYAIPFDIETLNKFYKNMETQELNLSEI
                     ISNPGFQAIAKAIRNSTVTLQFTPKEERMFDIRYGVAQELKSKSKSKSDLAEFIGEFI
                     SFYNAEIARNYEIKGKTYYRSTVKHEELDDFYALLDKYPSRLVGALLASYGFALSKRE
                     VKNDENKEENIKNDTENILKSNN"
     gene            4310..5284
                     /locus_tag="PKI65_07930"
     CDS             4310..5284
                     /locus_tag="PKI65_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HOB78399.1"
                     /translation="MENQKISSISISGEMILNLHSLNNEGGEGNQIMTRQLTIINKEG
                     EEYTVNGISGDMFKHIHTAHLINYAKVNNLNLCSNCKIGNPNRLSGGEELANFFSEKD
                     IIDKDVINAVIEKCIIDDCHGVLITSIGKTNKNVARKSIIEFGWTIGIPDKNNTETFL
                     HTKLVSDATGKRGESSNEGQNIFHRPANHGIYAFVCNIDVYRIGLNDITRVYAIDENE
                     RINRFKGIIQSLINSFLDPKGAMTSTQKPHITDFRGVVAISKKLIPAPTISAINPDYK
                     EEITKIAENLNNIENDSINLSEFNGLGDLSKILADLMNYEPYSIPKIS"
     gene            5291..5953
                     /locus_tag="PKI65_07935"
     CDS             5291..5953
                     /locus_tag="PKI65_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78400.1"
                     /translation="MKFSAIYKPTTLFSLRDSNSTSSGGKSLFLPSPYSIRMAFISQS
                     ISLGGVDFEENKDLFDIIKNVNISYFIKGTFCVNNCFVKIQKQRENEPFKPTIGFREY
                     IYIDNEIEIIFETNNEKETNFLKTYLHTINYFGKRGCFFQFIKYNDTPSEANVKILDE
                     KNFNYYGILQEFDDFHTSVKFSNINTYSQEKTKRIKNIYVIPLSHIRSSKSYSLYKTL
                     NY"
BASE COUNT         2544 a          655 c          827 g         1978 t
ORIGIN      
        1 atggtagagg taacaaaaga aaataatagc aatgttggta aagaattgat ataaagtaat
       61 ttatgaatag ttatactgat ttttataaaa aaataacggg gtttacccca tatgaatacc
      121 aaaaaaatgt agctgaaata cttttaaatt cagataaaaa tataattctc tcagtaccaa
      181 caggtgcagg caaaacctgg gcatcaataa tgccttattt gtatgctaga caatgtaatt
      241 atgaaaattt cccacaaaaa ttaatctata gtttaccatt gcgaacatta acaaattcta
      301 tatataacga tattaatgaa atattaaata aagatgaaat aaaagggaat tatattttta
      361 ataatttgac ttctattcag actggtgaat atagtgaaga tgtttatttt gaaaaagaca
      421 tgattttcgc gacaatagat cagactttaa gtaacttttt gtgtttccct ctttctcttt
      481 cgcaaagaca agcaaatata aatgctggtg cattaatagg aagctattta atattcgatg
      541 aatttcatct tttagatcct caattatcaa tgtcaacact cataggaata ataaaaatgt
      601 tggaaggcat tagtcgtttt tgtttaatga cagctacatt aacagataaa tttttgaatt
      661 tttttaatga gaatttaaat tttgagatag tttctcttga aaattttcct aaagataaag
      721 aaaatataaa atctataata tttaatccta ataaaaataa tacacttaat gaacaaatac
      781 ataaaaaaga aatacaggta gttaataata ctataaatgt acaaaatatt ttacgtgaac
      841 ataaaaataa aacaatagtt atatgtaata gaattgaaac cgcacaaaac ctttacttag
      901 atttaaaaaa gcaaaaaaat gatagtacta cattaatatg tattcattct agatattttg
      961 attctgatag gcaaaataaa gaaaaattaa taaaagaata ttttagtaaa aatagtgaaa
     1021 aacaagatgt aatattagtt tctacacaag tattagaagc aggtatggat atttcttgcg
     1081 atactatgca tactgaaatt tcaccaatta attcatttat acaaagagct ggtagatgtg
     1141 ctagatatga aaatgaatgt ggtaaaatat ttgtttataa catattagat ttgtcagaaa
     1201 aggaactagt taatgtagat tctgtaaacg atattgataa gacagaaatt aaaaaattga
     1261 ctattaagta tttgccttat gacaaagaaa cttgcgaaat ttcttttaaa gaattacaac
     1321 aatatgatca tttagatgaa tctattacaa aaaccataat aaatgatgtt ttaagtaaaa
     1381 cggagcaaaa tataattgaa aatattcatg caaaatcata tagtaaagag aaaattagaa
     1441 aatcttggca agattgcaca aaaaatcatt atagtgaaac catacgcgat atacaaagta
     1501 tagatgtagt tttgctaaac atcgataatt ttaaaaataa aaaaatacaa ccttttattt
     1561 atgaaactat tagtgttttt agatggagtt taataggatg gataaagaaa atagagcaaa
     1621 gtattgaaga agatgaatgg ataatagcta aggcagaaca aggtttacaa agtcaatttg
     1681 attttgactg gcaagataaa gaaagctatt ttttgagaaa attaaatact aatgatttaa
     1741 aaaactatta tgacacagtt tttattagta acaaatattt tgattataac gacgctggat
     1801 taatcattca aaataataat aacaataatg aatcaccaat aaaagaatat gagaaaaaag
     1861 aaaaggggag tattattttt aaaaaagata cattttatca gcatacactt gggttattaa
     1921 attgttataa tcaagtattt aagcctaatt taaaatatac aataaatagg ttaaaaaaaa
     1981 tttgtcctaa tattaattta gagctgataa taaaactagt tttaattttt catgattacg
     2041 gaaagctaaa caaatcatgg caagaaccta tgcaaaaatt tcagagtaaa aaaactggta
     2101 tcccgtgtaa tgaagtacta gcacatacag attatgatga acttacagac aaagaattag
     2161 cacaaaattg cggattaaat aaaaaaccat ctcatgctgg catcggtgct atacaattat
     2221 ataacatgat ttttgatttg tacaatgaaa atatagcaag agctagtagt agcgctattt
     2281 tgagacatca tagtccttta tcagattctt ttgagagttt tgatattccc gaaaaatatt
     2341 tgatagatat taaaaatttg ataaatgaaa taggaataga atgtgaatac ataaaaaagg
     2401 caaatggaga ggatttaaaa gaaattttac ccaataatga tttagaacaa attttttatt
     2461 tatttttggt aagaatttta cgtctttgtg atcaaaaagt agcagaatca ttaaataaat
     2521 atatgatatg aatacattta tagttaataa aatttcagga acttattctg atgctttaga
     2581 agcatatgga ttggctaatt tacttaataa aatctttgat aatagggaca taattctaga
     2641 aaaaatcatt atagaagata aaggtttata ctttcaaatt actatttcac cagatattac
     2701 tagtgaagac atagataaat tatcattttt ttcattattt ccatttataa agcaaaataa
     2761 tgatatagat atttctatat atcctgatta ttttgattat attaaacaaa aagaacttaa
     2821 aaaagaaaaa caagaattaa tcaataaaat ttacaatgaa tacaaagata aaaatgaaag
     2881 agaaaaaaaa ttgaaagaaa ttgataatat ttatcaaaca attaaatata tagatccttc
     2941 actagatgtt tttcaacaat ttgcaagcta taataattat ataacttatg aaaaattata
     3001 tttgaatttt tataacaata aagatatctt taataaatta attaaagaaa ttttacaata
     3061 ttattctgat aaaaattata ctgacaaaac attaaaagag gttataaata atagagattt
     3121 taatataaaa attacagcta ttcaattact aaatccaagt aaagggaaag gtattaagtc
     3181 agcaaaagca aataatgcgt ccaatactaa ttttaataat tctaattgga ttgaagaaac
     3241 tatgaaaatt atagggtcat tatcagatat ggtttgtcaa ttagtcaaag taggtactaa
     3301 tagctatgat cttaaagtat ttgtaccttc ttataaaaaa gtaagttatt cttttaaaca
     3361 aaaaataatt cctgatttta agaatcattt aaaaggcaat acacctatta aaatagatat
     3421 actgaatatt ttaattttaa taaaaattgc tatacaacat ctgggtaatt ctataataaa
     3481 acaaaaagtt aataacataa tagacggcct ttattcagtt tatcaaaaag atttaggaca
     3541 aggcaaagca gtatcgaata ttagttattt gcaaataccc aattttattt atataagtaa
     3601 taaaatagaa aatgatgaat gggttgatat tttagatgaa caaataaata taataggcag
     3661 tattgatgaa caaggtgaaa caatacaagg tttaatgcta tacagaaatt ttattagcac
     3721 atcaagtatt tatgattttt taaatttctc atattggtat gctacatatt tatcttctaa
     3781 actttctaaa aatgaatatg caataccatt tgacatagaa acattaaata aattttataa
     3841 aaatatggaa acacaagaat taaatttgtc agaaatcatt agcaatcctg ggtttcaggc
     3901 tatagctaaa gcaattcgta atagtacggt tactttacaa tttacaccaa aagaagagag
     3961 aatgtttgat attagatatg gagtagctca agaactaaaa tctaaatcaa aatcaaaatc
     4021 agatttagca gaatttatag gtgaatttat atctttttat aatgctgaaa ttgcaagaaa
     4081 ttatgaaatt aaaggcaaaa cttattatag atcaactgtg aagcatgagg agttggatga
     4141 tttctatgca ttattagata aatatccatc aagacttgtt ggcgcattac tagcatcata
     4201 tggttttgct ttatctaaac gtgaggtaaa aaatgatgaa aacaaagaag aaaatatcaa
     4261 aaatgataca gaaaatattt taaagtcaaa caattaaaaa attataatta tggaaaacca
     4321 aaaaatttca tcaatctcca taagtggaga aatgatatta aatcttcatt cacttaataa
     4381 tgaaggagga gaaggaaatc aaattatgac tcgacaatta acaattatta ataaagaagg
     4441 cgaggaatat actgtcaatg gaataagtgg agatatgttt aagcatatac atactgcaca
     4501 tttaataaac tatgccaaag ttaataattt aaatctttgc tctaattgta agattggcaa
     4561 ccctaatcgc ctgtctggtg gggaagaatt agctaatttt ttctctgaaa aagatataat
     4621 agataaagat gtaataaatg cagtcattga aaaatgtata atagatgatt gccacggtgt
     4681 actaataaca tctattggta aaacaaataa aaatgttgca agaaaatcaa taatagaatt
     4741 tggttggact ataggtatac ctgataaaaa caatactgaa acttttttac atactaaatt
     4801 agtttcagat gcaactggta aaagaggtga aagttctaat gaaggacaaa acatatttca
     4861 tagacctgct aatcatggga tatatgcatt tgtttgtaat atagatgtat atagaattgg
     4921 attaaatgat ataacaagag tttatgctat agatgaaaat gaaagaatta atagatttaa
     4981 aggcattata caaagtttaa taaattcatt tctagatcct aaaggtgcta tgactagcac
     5041 tcaaaaacct catattactg attttcgtgg tgtggtagct atttctaaaa aattaatacc
     5101 tgctcctact ataagtgcaa ttaatccaga ttataaagag gaaattacta aaatagctga
     5161 aaacttgaat aacattgaaa atgattctat taatttatca gaatttaatg gattggggga
     5221 tctttctaaa atattggcag atttgatgaa ttatgaacct tattcaatcc ctaaaataag
     5281 ttgaaaaatt atgaaatttt cagcaattta taaaccaaca acattattct cattgcgaga
     5341 tagcaactca actagctctg gtggtaaatc tttattttta ccttctccat attcaattag
     5401 aatggctttt attagtcaat ctatctcgtt aggaggtgta gattttgaag aaaataaaga
     5461 cctatttgat attattaaaa atgtaaatat cagctatttc attaaaggaa ctttttgtgt
     5521 aaataattgc tttgtaaaaa tacaaaaaca aagagaaaat gagcctttta agcctactat
     5581 tggatttaga gaatacattt atattgataa tgaaatagaa ataatttttg agactaacaa
     5641 tgaaaaagaa actaattttc taaaaactta tttacacact attaattatt ttggtaagcg
     5701 tggttgtttt ttccaattta ttaaatacaa tgatactcca agtgaagcta atgttaaaat
     5761 attagatgaa aaaaatttca attactatgg tatattacaa gaatttgacg atttccatac
     5821 atctgttaag ttttctaata ttaatactta tagtcaagaa aaaacaaaaa gaattaaaaa
     5881 tatttatgta ataccattat ctcatattag atcatcaaaa tcctattcac tctataaaac
     5941 tttaaattat taaattaaaa cattttatgc cttcaaaatc tgaattaaaa aagattggtt
     6001 ccat
//
LOCUS       DAOPRN010000091         5952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_10892_length_5952_cov_114.122605, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000091 DAOPRN010000000
VERSION     DAOPRN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10892_length_5952_cov_114.122605"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..3390
                     /locus_tag="PKI65_07940"
     CDS             178..3390
                     /locus_tag="PKI65_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOB78401.1"
                     /translation="MENKYINSDITFITNEEGNKLKNRFNNLIKDSKEFLSLSGYFYL
                     SGFHQIYKSLDHVDKIKILVGIGTDKKTLDQIEKSKNEILFSDPNIKEKSEELILNEL
                     ENIDDKKDVEISIKKFIEWIKNGKLEIKAYPSQKIHAKLYIFTFRDTDRDKGRVITGS
                     SNLTQSGLLENLEFNVELKNFSDYKFALNKFNELWEQAVDLNDNYVLNISNKTWIKDD
                     ITPYELYLKFLYEYFKDELNVSSEIFNNYVPQDFKRLKYQEQAVLNAKRILNEYGGIF
                     LSDVVGLGKTYIAAMLAGQLDGRTMVLAPPSLINKNNPGSWPNVFYDFNIPANFVSIG
                     NLDEAKRIMENREFKNIIIDEAHRFRNENTISYEKLMEICRGKRVILVTATPFNNSPK
                     DLLALIKLFQNAKNSNIPGVEDLESFFKELDKKLDKIDRQKNNEKYIETIQSNAAEIR
                     NKVLKYIMIRRTRSEIEKYYVDDMKKNNIIFPEIQKPLPLYYLLNETEDEIFDKTLRA
                     ITTDFKYARYTPLLYLNDKLSSLEIQSQKNMSSFMKILLVKRLESSFFAFNKTIDRFI
                     KSYENFIKAYNNNIVFISKKYIKKILEYIDNDDYDKIYKIIEDGNAKKYDARDFTPNL
                     ITDLNNDLQILKTIKNDWGKIRRDPKLNTLIYNLNHNNILKNSKIIIFTESKETAEYL
                     AEKINNTLNDKALLFHSESQEIIKNEVINNFDANAKNQKNDYKILISTDVLSEGVNLH
                     RSNVVINYDIPWNPTKLIQRVGRINRVNTPFDKIYTFNFFPSKQSEKEINLEIIAREK
                     IVNFFTLLGGDSNILTEGEPVSSHEIFDKLTSIDNLVDNEQEVSELKYLKIIEEIKEK
                     QPDIYEKIKKLPKKARSSKKIPNNINCNINKNALLSFIKIDKLKKFYITIDGNEPKEL
                     DFISAAQILESLENEPKLSDIDIDKYYNMLYKNKNAFDYDNDQNVLLNNKTYGIYNKL
                     YNNIKAIQSNFTYQMTDEQNNYLLNLLNRIYDKSIPYNTIKKILQEFRKLGRDAANPK
                     IVLDMLQSNIPDNLLESHLAEKNKNENSKKEIILSLYLKK"
     gene            3394..>5952
                     /locus_tag="PKI65_07945"
     CDS             3394..>5952
                     /locus_tag="PKI65_07945"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOB78402.1"
                     /translation="MEVTRASKIIKDAFESQFNKENFYNFINQLLKNIEGTTFIYKGA
                     YIKDNYKDFVKTFERVGKFSTNNKEIDILIVQLKDAKCLERARSKQRNFIASYLNGSM
                     GGKQKDAALVAFYAPDAVDWRFSFVKMDYNITVIDDNIKVKEQFSSAKRFSFLVGKNE
                     KIHTAESRFLPLIISDNKPSFTDIEKAFDVEIVSDEFFKKYRDLFVNLKSELDKLYSK
                     DEKLQQEFNSKNITTVDFAKKLLGQIIFLYFLQKKGWFGVAKNQSWGTGPKDFIRKLF
                     AGDYVSYDNFYTEILEPLFYEALRTDRAFNNHFYDKLQCKIPFLNGGLFDPINNYDWL
                     NNEIKIPNEVFSNNNKTKEGDIGDGILDIFDKYNFTVNEDEPLEKEVALDPELLGKIY
                     EKLNAIRDDNFDEYYIALKSGRVGEETKFNKEYGVYYTPRDIVHYICQKSLINYLFTN
                     LNGKVPKKDIMSLVKNTEINEEYETIAIEKEQKIKQGDQKHSRYCSKIPDSIKENAKD
                     IDQLLANITVCDPAIGSGAFPIGMMHEIVKLRQLLSVHTANQINTYDLKRYCIENSLY
                     GVDIDLGAIETCKLRFWLSLIVDEDDFADIRPLPNLDYKIVCGNSLLGYPNNYKPSGL
                     VNIEKLKKEFFDTTNPVEKNKLKKTIDESIDKLLKNTEESFGYRITMDFTINFSEVFS
                     HNGGFDLVIGNPPYISIKGYNQDDKQILKYLFGFADDFYSHFIFKGIDILKNNGILAF
                     ITSKTYWTIQTKKNLRELLLKNKILEIYDTGNPFDAPIVDTAIVIVQKNNEINDYKFI
                     FIDGKNDFMNPDVFVGDINLYKNAPNNVFFIPNETNIKIANKYFNQVQPLLDKWW"
BASE COUNT         2481 a          717 c          832 g         1922 t
ORIGIN      
        1 attgtagcgg caactcacga atcattagtt cataaaattt taattttaaa atagtgtgta
       61 aaaatcatta taaatttgta aaaaaaatta tttatctaca ataacacttt atagaaccaa
      121 taaaaatagt ttcttcatta tttatatttt ttttaaatta gcagaaatat tttttttatg
      181 gaaaataaat atataaattc agatattaca tttataacaa atgaagaagg caataaatta
      241 aaaaatagat ttaataatct aataaaagat agtaaagaat ttttatcttt atcagggtat
      301 ttttatctaa gcggatttca tcaaatttat aaatcattag atcatgtaga taaaataaaa
      361 atacttgtag gcattggtac tgataaaaaa acacttgatc aaatagaaaa aagtaagaat
      421 gaaattttat tttctgatcc taatattaaa gaaaaatcag aagaattaat attaaatgaa
      481 ttagaaaata ttgatgataa aaaagatgta gaaatttcta ttaaaaaatt cattgaatgg
      541 attaaaaatg gtaagttaga aataaaagca tatccttctc aaaaaataca tgctaaatta
      601 tatatattca catttagaga tactgataga gataaaggta gggtaataac aggctctagc
      661 aatttgactc aatctggatt attagaaaat ttagagttta acgttgagct taaaaatttt
      721 agtgattata aatttgcatt aaataaattt aatgaattgt gggagcaggc ggtagattta
      781 aatgataatt atgttttaaa catctctaat aaaacgtgga ttaaagatga cattactcca
      841 tatgaactat atctaaaatt tttatatgaa tatttcaaag atgaactaaa cgtatcatcg
      901 gagattttca ataattatgt gcctcaagat ttcaaaagat taaaatatca agagcaagct
      961 gtattaaatg ctaaaagaat tttaaatgaa tatggaggca tttttttatc agatgttgtt
     1021 ggtttaggaa aaacatacat cgctgctatg cttgctggac agctagatgg cagaactatg
     1081 gtgctagcac ctccttcatt aattaacaaa aataatcctg gctcttggcc aaatgttttt
     1141 tatgatttta atataccagc taatttcgtt tctattggta atctagacga agcaaaaaga
     1201 atcatggaaa atagagaatt taagaatatt atcatagatg aggcacatcg ttttagaaac
     1261 gaaaatacta ttagttatga aaaattaatg gaaatatgtc gaggtaaacg tgttatatta
     1321 gtcactgcta cgccttttaa taattcacct aaagatttat tagcactcat taaattattt
     1381 caaaatgcta aaaacagtaa tatcccaggt gtagaggacc tagaaagttt ttttaaagag
     1441 cttgacaaaa agctggataa aattgataga caaaaaaata atgagaaata tatagagact
     1501 atacaatcta acgctgctga aataagaaat aaagtgttga aatatatcat gataaggcgc
     1561 actagaagtg aaatagaaaa atattatgtc gatgatatga aaaaaaataa tattatattt
     1621 cctgaaatac aaaaaccact accactttat tatttattaa atgaaacaga agatgaaata
     1681 tttgacaaga ctttaagggc aataactaca gattttaaat atgctagata tactccactt
     1741 ttatatctta atgataaatt aagctctcta gaaattcaat ctcagaaaaa tatgagtagt
     1801 tttatgaaaa tattactagt caaacgttta gagagtagtt tttttgcttt taataaaact
     1861 attgatagat ttattaaatc ttatgaaaat tttataaaag cttataataa taacattgta
     1921 tttatcagta aaaaatatat taaaaaaata ttagaatata tagataatga tgattacgac
     1981 aaaatttata aaataataga agatggtaat gccaaaaagt atgatgctag agacttcacc
     2041 ccaaatttaa ttaccgacct aaataatgat ttacaaattt taaaaacaat taaaaatgat
     2101 tggggaaaaa tcaggcgtga ccctaaatta aatactctta tttataatct taatcacaat
     2161 aatattttaa aaaatagcaa gattatcatt tttacagagt ctaaagaaac cgctgaatat
     2221 ttagctgaaa agattaataa cacattaaat gataaagcat tattatttca tagtgaatca
     2281 caagaaatta ttaaaaatga agttattaat aacttcgatg ctaatgcaaa aaatcaaaaa
     2341 aatgattata aaatattaat atcaacagat gtactttcag aaggggtgaa tttacataga
     2401 tctaatgtag tcatcaacta tgatataccc tggaacccca ctaaactaat acaaagagta
     2461 ggtagaataa accgcgtaaa tacacctttt gacaaaattt atactttcaa ctttttccct
     2521 agtaaacaat ctgaaaaaga aattaattta gaaatcatcg ctagagaaaa aattgtaaat
     2581 ttttttactt tacttggcgg cgatagtaat atacttacag aaggagagcc cgtttcttct
     2641 catgaaatat ttgacaaatt aacttctata gataatttag tggataatga acaagaagtt
     2701 agcgagttaa aatatttgaa aattattgaa gaaattaaag aaaaacaacc tgatatttac
     2761 gaaaaaataa aaaaattacc taaaaaagct cgttcttcta aaaaaatacc aaataatatt
     2821 aattgcaaca ttaataaaaa tgcactactt agctttatta agattgataa attaaaaaaa
     2881 ttttatatta ctattgatgg taatgaacct aaagaattag actttatttc agctgctcag
     2941 atcttagaaa gtttagaaaa tgaacctaaa ttatcagata tagacataga taaatactat
     3001 aatatgttat ataaaaataa aaatgctttt gattatgata atgatcaaaa tgttttatta
     3061 aataataaaa catatggtat ttataataaa ctttataata atattaaagc tattcaaagt
     3121 aattttacct atcaaatgac agatgaacaa aataattatt tacttaatct gctaaataga
     3181 atttatgata aatcaatacc ttataatact attaaaaaaa tattacaaga atttagaaaa
     3241 cttggccgtg atgcagcaaa tcctaaaata gtattggata tgctacagag taacattcct
     3301 gataatttat tagaaagcca tctagctgaa aaaaataaaa atgaaaatag taaaaaagaa
     3361 attattctat ccctatattt aaaaaaataa tttatggaag taactagagc cagcaaaatt
     3421 attaaagatg catttgaatc acaatttaat aaagaaaatt tttataattt cattaatcaa
     3481 cttttaaaaa atatagaggg tactactttt atttataaag gtgcttatat aaaagataat
     3541 tataaagatt ttgtgaaaac ttttgaaaga gttggtaaat tctcaactaa taataaagaa
     3601 atagatatcc taatagtgca actaaaggat gcaaaatgtc tagaaagagc ccgatctaag
     3661 cagagaaact ttatcgcttc atatctaaat ggatcaatgg gtggcaaaca aaaggatgca
     3721 gctctcgttg ctttctatgc ccctgatgct gtagattggc gtttctcttt cgtgaaaatg
     3781 gattataata taacagtaat tgatgataac atcaaagtaa aagaacaatt tagctctgct
     3841 aaaagatttt cttttttagt aggtaaaaat gaaaaaattc atactgctga gagtagattt
     3901 cttcctttaa taatctctga caataaacca tcatttactg acatcgaaaa agcttttgat
     3961 gtagaaatag tctctgatga atttttcaaa aaatatagag atctttttgt caatctcaaa
     4021 tctgaattgg ataaattgta ttcaaaggat gaaaaattac aacaagaatt taattcaaaa
     4081 aacatcacta cagtagattt tgcaaaaaaa cttttaggac aaataatctt cctttatttc
     4141 ttacaaaaga aagggtggtt cggtgtagct aaaaatcaat catggggcac tggacctaag
     4201 gattttatac gtaaactatt cgcaggtgat tatgtatctt atgataattt ttatacagaa
     4261 atcctagaac ctctctttta tgaagctctg agaacagata gagcttttaa taatcacttt
     4321 tatgataaac tacaatgtaa aatacctttt ctaaatggtg gactctttga tccaataaat
     4381 aactatgact ggttaaataa tgaaataaag ataccaaacg aggttttttc taataataat
     4441 aaaacaaaag aaggcgatat cggtgatggc atattggata tttttgataa atataacttc
     4501 actgtcaatg aagatgaacc attagaaaaa gaagtagcat tagatcctga actattaggt
     4561 aaaatctatg aaaagttaaa tgctattcga gatgataatt ttgatgaata ttatatagct
     4621 ctcaaatctg gtagagtagg agaagaaaca aagtttaaca aagaatatgg cgtgtattat
     4681 actcccagag atatagtgca ttatatctgt caaaaaagcc taataaatta cctatttact
     4741 aacctgaatg gtaaagtccc aaaaaaagat attatgtctt tagtgaaaaa tactgaaatt
     4801 aacgaagaat atgaaaccat agctatagaa aaagaacaaa aaattaaaca aggtgatcaa
     4861 aaacattcac gttattgtag taagatacct gatagcataa aagaaaatgc aaaggatata
     4921 gaccagctat tagcaaacat tactgtgtgc gatccagcta taggttctgg tgcctttcca
     4981 ataggtatga tgcacgaaat agtgaaacta agacaattat tatctgtgca tactgctaat
     5041 caaataaata cttatgacct aaaacgctat tgcatagaga actctttata tggcgtagat
     5101 atagacctcg gtgctataga aacatgtaaa cttagatttt ggctatcact aattgtagat
     5161 gaagatgatt tcgcagatat tagaccacta ccaaacctcg attataagat cgtctgtggt
     5221 aactcccttt tgggttatcc taataattat aaaccaagcg ggctagtaaa tattgaaaaa
     5281 ttaaaaaaag aattttttga tacaactaat ccagtagaaa aaaataaatt aaaaaagact
     5341 atagacgaat ctatagataa gttattaaaa aatactgaag aatcttttgg ctacagaata
     5401 actatggact ttacaattaa tttttcagaa gtattttctc ataatggagg ctttgatcta
     5461 gtgattggca atccccccta tataagtata aaaggttata accaagacga taaacagata
     5521 ttaaaatatt tatttggctt tgcagatgac ttttattcgc attttatttt taaaggtatt
     5581 gatatactta aaaataatgg catattagca tttataacat ccaaaaccta ctggactatt
     5641 caaacaaaga aaaatttgag agaacttcta ttaaaaaata aaattttgga aatttatgat
     5701 actggcaacc cctttgatgc tcctatcgta gatactgcaa tagtcattgt tcaaaaaaat
     5761 aatgaaatca atgattataa atttattttc atagatggga aaaatgattt tatgaaccct
     5821 gatgtttttg taggagatat aaatttgtat aaaaatgctc caaacaatgt tttttttatc
     5881 cctaatgaaa caaatataaa gattgcaaat aaatatttta atcaagttca gcctctttta
     5941 gataaatggt gg
//
LOCUS       DAOPRN010000092         5894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_11038_length_5894_cov_125.692242, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000092 DAOPRN010000000
VERSION     DAOPRN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11038_length_5894_cov_125.692242"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..371
                     /locus_tag="PKI65_07950"
     CDS             189..371
                     /locus_tag="PKI65_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78403.1"
                     /translation="MRRFRGVATKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
     gene            774..1823
                     /locus_tag="PKI65_07955"
     CDS             774..1823
                     /locus_tag="PKI65_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOB78404.1"
                     /translation="MKILITGTAGFIGFHLAKKLLERGDTVIGIDNINDYYNVNLKYA
                     RLLETGIDSRATEWHLKIQSKKYPNYTFIRMNLEDRESLLRLFATEHFDNICHLAAQA
                     GVRYSLQNPYAYIDSNIVGFINILEGARNNNIKHLVYASSSSVYGGNTKMPLCTTDNV
                     DHPISLYAATKKTNELMAHVYSHLYNIPTTGLRFFTVYGPWGRPDMALFLFTQAILEG
                     KPIKVFNYGNMTRDFTYIDDIIEGVIRVIDKPAKPISIHDPDPSSSNIAPYKVYNIGN
                     ANPVKLMDYIEAIEKNLGLKAKKEFLPLQPGDVPHTEADVSDLVRDFGYKPTTNVEDG
                     IREFIEWYKEFFLIK"
     gene            complement(1898..5416)
                     /locus_tag="PKI65_07960"
     CDS             complement(1898..5416)
                     /locus_tag="PKI65_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOB78405.1"
                     /translation="MKYGVTWWGKRWLNSLNRIDYSNRLERGLSYADNGFVVYISTVA
                     NKIIAKVQGTRDTPYNVSIEIPEFSDEQKILLINIIKHNPQILSKLLNNEISPELETL
                     ALNNGIKIFPTSWKDFKMRCSCPDWVVPCKHIAAVLYLFANEIDKNPFILFNLHGLDL
                     LKEFHNEGINIQNTGIASFEDLFTKSLNKVNISDDMARLYNIDLSELSSDRYSPFSLL
                     SPSPVFYYKDFKELFIVTAIKLSKYVEKKFINNNKIKNFNILPLAIDSDINFLLDYKT
                     LKTSIELVYSNHKTLISVDDFIDYLQFIQYKHLSLLNDSWLLIYKIYLFSLQLVIKKL
                     YLPRILKYSNDIYVLQYIPASIEDSVKNIFDEISQYLSLDIIKKQNNYKKEYLKDQSQ
                     ELNFLCSIFINYFFEKAYNDNQFSPEWDNKELYYGIRELFINGVSDNFDVFTTKEIPQ
                     SIHQWLSIFDIHTRRFVPLLKIDRPERESEVGFRLSILISDTINEDSMYIELRDIIEN
                     EKYDSIRLEILKDLSIVANYIPQINDLLSDTNKTIVWLPFEEFPDILNNIIPIIKMLG
                     VKVLLPKELKELVHPYLLLQINNIPELRGGELLTIDALTNYKWQVCIGDEQIDAEEFF
                     ELVKNLNGFVKLKDRYIFLNESDINSLRKNFEELLKYGGILGPLAAVDGEYLGNEIQI
                     APEIKDQLKDLLKVKEIPIPQALNATLRPYQVRGYEWLVRNSKLGLGSLIADDMGLGK
                     TIQVITLLLHYQEKELLQQALVVVPTSLISNWEQELSKFAPTLKYYSYYGNKRKKDFD
                     NYDVIITSYGIIRNDLEAFYDKKWQFLIIDEAQNIKTVSSKQTKDIKKLQADIRIAMT
                     GTPVENRLTEYWSIFDFINTGYLGSYYEFKEKYVKPIHYNHDKDCIERFRRITSPFIL
                     RRLKTDKTIISDLPEKIENNYYTNLTKTQAALYQSVVNVTMEKLNAMPFDDNDRAVFV
                     LKLILDLKQISNHPYQFLKQGSKHYSKSGKALLLLNLVENIVDYGEKVLIFTQFKEMG
                     QLLVEFIKDRIGQEPLFLHGSCTRKQRDDMIWKFQNSFPQVFILSIKAGGKGLNLTAA
                     NHVIHYDLWWNPAVEAQATDRAFRIGQKKNVMVYRLINRGTIEEKIDKLLKEKKELAA
                     LTVNTGENWIGNLNNDELKELLRLEN"
     gene            5605..>5894
                     /locus_tag="PKI65_07965"
     CDS             5605..>5894
                     /locus_tag="PKI65_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOB78406.1"
                     /translation="MDSQRHWFVLKTKYRCEKKLYQQLQNDGYEVCLPMYSTVRQWSD
                     RKKKLQLPLINCMVFIKTNTKELYKIYDYPLVRGVLKEFGKPAIVRDEEIKNL"
BASE COUNT         2055 a          880 c          803 g         2156 t
ORIGIN      
        1 tttaaataaa tagatccagg aaatttagct ttgtaaaaat tacgcttcat aaaggcgatt
       61 tgtgaacgga gctacttaaa aatatcttct gttttacttg aagtagttta tagcataatt
      121 aaataattag acgcggtgta tacagtgtac aggtagtcga taagcatata gatgatttca
      181 aggtttggtt gcgacgattt agaggagtgg ctactaaata cctccagagc tatttgatgt
      241 ggtttgtaat cacacataaa tacttgacaa gtgagataga atcagacatt ggtcggcttt
      301 taaacctcgc atcctcggac agatgggcgt gggatgagta ttggcggctg gtaaatacaa
      361 agtatagata aactacttca agtaaaacaa tgatttgttt ctagaccctt ttcgattcca
      421 ctatgcacta atttagttga actaaaatag tgcataaatc acatcacaac taaaaaattt
      481 atcgaataga ttaattattt tatatttcac attctcaaaa ttgatttatt ttaatcaata
      541 tgataatttc tttatactta gttaataata tttcaaggcg aattaatggt tttaaaaaaa
      601 attgtaattt agctattcaa aaaaaaatga aattctaaga taatttttag ttataagttg
      661 attaattatg atttaattat gagatatata taagttaaaa aaatcataac cattatgaat
      721 gaatatattt ttttaaactt tacattaatt attaaacatt aaattatata cagatgaaga
      781 tactcatcac tggtacagca ggatttatcg gttttcacct tgctaaaaaa ttattagagc
      841 gaggcgacac tgttatcggc attgataaca ttaatgatta ttacaatgta aatctaaaat
      901 atgctaggct tttagagaca ggtatagata gcagagctac agagtggcat ttaaaaattc
      961 aaagtaagaa atatcctaat tatactttta taagaatgaa tctagaagat agagaatctt
     1021 tgcttagact ttttgctacc gaacatttcg ataatatttg ccatctggca gcacaagccg
     1081 gagtacgtta tagcttacaa aatccttatg catacataga tagtaatata gttggattta
     1141 ttaatatact agaaggtgca cgaaataata atatcaaaca tttagtatat gcgagctcat
     1201 caagtgtata tggtggcaat actaagatgc cgctatgcac taccgataat gtggaccatc
     1261 ctatcagttt atatgctgct actaaaaaaa ctaatgaact aatggcacac gtttacagtc
     1321 atctatataa tattcctacc actggtttga gatttttcac agtttatggg ccgtggggca
     1381 gaccagatat ggcattgttt ttattcactc aagcaatttt agaaggcaaa cctattaaag
     1441 tttttaatta tggtaatatg actcgagact ttacttatat agatgatata atagaaggtg
     1501 taatcagagt aatagataaa cctgctaaac ccatctctat tcatgatcct gacccttcat
     1561 ctagtaatat agctccgtat aaagtttata atatcggtaa tgctaatcct gtaaaattaa
     1621 tggattacat agaggcaata gaaaaaaact taggcttgaa agctaaaaaa gaatttcttc
     1681 cattacaacc tggtgatgtg cctcataccg aagcagatgt atcagatctt gtgagagatt
     1741 ttggttataa gcctactact aatgtagagg atggcattag agaatttatc gaatggtata
     1801 aagagttttt tttaattaaa taactgctgg ctacaaatta actaataata agttaaatat
     1861 aaacgtttta ttttactact aaacttactc acaatcatta attttctaat ctcaacaatt
     1921 cttttagctc atcattgttt agatttccta tccaattctc acctgtattt actgttaacg
     1981 ctgctaattc tttcttttct tttagcaatt tatctatttt ttcttctata gtacccctat
     2041 ttattagtct atataccatt acattctttt tttgtccaat tctaaaagcc ctgtcagtag
     2101 cttgggcttc tactgctgga ttccaccata gatcatagtg tatcacatga ttagctgcag
     2161 tgagattaag tccctttccg cctgctttga tagataagat aaacacttga ggaaaactat
     2221 tttgaaattt ccaaatcata tcatcacgtt gcttacgtgt acagctacca tgtaaaaata
     2281 atggctcttg accaattcta tctttgataa attctactaa caattgcccc atttctttaa
     2341 attgagtaaa tatcaaaact ttttcgccat aatctacaat attttctact aaatttagca
     2401 aaagcaacgc tttacctgat ttagaataat gtttagaacc ttgttttaaa aattgataag
     2461 gatgattaga tatttgttta aggtctagaa ttagttttag aacgaacact gcacgatcat
     2521 tatcatcaaa aggcatagca tttagttttt ccatagtaac atttacaaca ctttgataga
     2581 gagctgcttg tgtctttgtt aaattagtat aataattatt ttctattttc tccggtaaat
     2641 cagaaattat agttttatca gtctttaacc tccttaaaat aaatggagag gtgatacgtc
     2701 taaacctttc aatacaatct ttatcgtgat tataatgtat cggtttcaca tatttttctt
     2761 taaattcata atatgatcct aaatatccag tattaatgaa gtcaaaaatg ctccaatatt
     2821 ctgttaaacg gttttctact ggtgtaccag tcatagctat acgtatgtca gcttgcaatt
     2881 ttttaatatc ttttgtttgt ttagatgata cagttttaat attttgcgct tcatctatga
     2941 ttaaaaattg ccatttttta tcataaaaag cctctaaatc attacgaata atcccataag
     3001 aagtaataat aacatcataa ttatcaaaat ccttttttct tttattacca taatatgaat
     3061 aatattttag agtaggtgca aatttagaaa gttcttgctc ccaattagat attaatgatg
     3121 ttggtactac tactaaagct tgctgtaata attctttttc ttgataatgc aaaagtaatg
     3181 tgatcacttg tatagttttt cccaaaccca tatcatcagc tattaagcta cctagtccta
     3241 attttgaatt tctaactaac cattcgtaac ctcttacttg atatggtctc aaagtagcat
     3301 ttagagcttg tggaattggt atttctttta ccttaagtaa atcctttaat tgatccttaa
     3361 tctctggggc tatttgtatt tcattaccta aatactctcc atctactgcc gctagtggtc
     3421 ccaaaatacc gccatatttc agaagctctt cgaaattttt tcttaaagaa tttatatcag
     3481 attcattcaa aaagatatat ctatctttta gttttacaaa accattgaga ttttttacaa
     3541 gttcaaaaaa ttcttctgca tctatctgtt catctccgat acatacttgc catttataat
     3601 ttgtcaaagc atcaatagtt aataattctc caccccttaa ctctggtata ttatttattt
     3661 gtagtaataa atatggatgc acaagttctt tcaactcttt tggtaataag actttcacac
     3721 cgagcatctt aattattggt atgatattat ttaatatatc aggaaattcc tcaaatggca
     3781 accacacaat ggttttgtta gtatctgata ataaatcatt gatttgtgga atataattag
     3841 ctactatgct gagatcctta aggatttcta atcttatact atcatatttt tcgttttcta
     3901 taatatccct tagctcgata tacatcgaat cttcatttat agtatcagag atcagaatag
     3961 acaatctaaa acctacctca gactctcgct ctggacgatc tattttcaat aatgggacaa
     4021 atctacgagt atgtatatca aaaatgctaa gccactgatg tatactctgc ggtatttctt
     4081 ttgtagtgaa gacatcgaaa ttatctgata caccattaat aaaaagttct ctaataccat
     4141 aatataattc cttattgtcc cattctggtg aaaattgatt atcattgtaa gctttttcaa
     4201 aaaaataatt tataaatata gaacagagaa aatttaactc ttgactttga tcttttaaat
     4261 attctttttt ataattgttt tgttttttta taatatctaa actcaaatat tgacttattt
     4321 catcaaaaat attttttaca gagtcctcta tacttgctgg tatgtattgt aatacataaa
     4381 tatcattact atattttagt atcctaggca aataaagttt cttaattact aattgcaatg
     4441 aaaaaagata aattttataa attagtaacc atgagtcatt tagtaaggat aaatgtttat
     4501 attgtataaa ttgtaaataa tctataaaat catctacact tattaaggtt ttatgattac
     4561 tataaactaa ctcaatagat gtttttagtg ttttatagtc taaaaggaaa tttatatcac
     4621 tatctatggc taaaggtaag atattgaaat tttttatttt attattatta ataaattttt
     4681 tctcaacata ctttgatagt tttatcgctg ttactatgaa taattctttg aaatctttat
     4741 aataaaatac tggtgacgga gataatagcg aaaatggaga atatctatca ctacttagtt
     4801 cagatagatc tatattataa agtctagcca tatcatcact aatgttaact ttatttaatg
     4861 atttagtaaa taaatcttca aagctagcta ttccagtatt ttgaatgtta ataccttcgt
     4921 tatgaaattc ttttaaaagg tctaaaccgt gtaaattgaa taaaataaat ggatttttgt
     4981 ctatttcatt agcaaaaagg taaagaactg ctgcaatatg tttgcatggc accacccaat
     5041 cgggacagct acaacgcatt ttaaaatctt tccatgaagt agggaaaatt ttaattccat
     5101 tatttagtgc taaagtttct aactctggac taatttcatt atttaaaagt ttagacaaaa
     5161 tttgtgggtt atgtttaata atatttatta ataaaatttt ttgctcatca gaaaattcag
     5221 gtatttcgat agatacatta taaggagtat ctcttgtgcc ctgtactttg gcaataattt
     5281 tatttgctac agtagagata taaactacaa aaccattatc agcataagag agtccacgtt
     5341 ctagacgatt actataatct atcctattta atgaatttaa ccatcgctta ccccaccagg
     5401 taacaccata tttcatatcc cacaattaaa ttattacaat attaataatt ttttataaaa
     5461 taaaaaagca tgttatattt aaaaataatt ttattagaaa atcaattgtc cgaaatatat
     5521 ttcggttatt aactaaacgc cttactcaaa taattattaa aaaaatatat gataagttta
     5581 ttaattttac aaaaaaattt ttttatggat agtcaacgac attggtttgt attaaaaact
     5641 aaatatcgat gcgaaaaaaa actttatcaa caattgcaaa atgatggata tgaagtttgt
     5701 ctaccaatgt atagtactgt acggcaatgg agtgatagaa agaaaaaact tcagctaccg
     5761 ctcattaatt gcatggtttt tataaaaaca aatactaaag aattatataa aatttatgat
     5821 tatcccttgg ttagaggcgt tttaaaagaa tttggtaagc cagctatagt gagagatgaa
     5881 gaaattaaaa acct
//
LOCUS       DAOPRN010000093         5495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_12079_length_5495_cov_133.944669, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000093 DAOPRN010000000
VERSION     DAOPRN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12079_length_5495_cov_133.944669"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKI65_07970"
     CDS             <1..251
                     /locus_tag="PKI65_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78407.1"
                     /translation="YSLSTVKKKASEFTNWIYFKFRGVATKYLQNYIMWYVVMGKYLT
                     GKTVSNTGRMLNLASSDRWAWYRYRELVNITYVNLNIY"
     gene            complement(626..2284)
                     /gene="recN"
                     /locus_tag="PKI65_07975"
     CDS             complement(626..2284)
                     /gene="recN"
                     /locus_tag="PKI65_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOB78408.1"
                     /translation="MLQHLDIKNYLLIHNLSLDFYNGFTVITGETGSGKSILLGAIQL
                     LLGERADSGALLDKSKKCVIEGVFDIKQLDLKHFFEQNDLEYEDTLIIRREISPNGRS
                     RAFINDTPVNLSILKSIGEQLINIHSQFDTLLLGTKKFQYTLLDSFANTNDLFTKYLK
                     LYENFTFFKKEIEEDREMMRQLMHENDYKQFQWEELKNADIKENEYEELHQQYELLSH
                     SELTLQTIKETTALLYENEQNIYQNIAQIIQKLRNISSYLPKAEKFIDILENIEIEIK
                     DISMSLSELADSLDYSPDELQRIENRLDLFQKLMFKHHVQTTEELLELQENLKKDIKN
                     IDELANKIVKKEANLLQIETELRDTAIKLYNERKKAIPELEKLIIKLISRLGMQDGKF
                     IIQLEKLDEPTIFGFDKLELKFSANKGIEPEALERVASGGEMSRLMLALKSIIASQNY
                     LPTLIFDEIDIGVSGNIAAQMADLLSDMSMHHQVIVITHLPQIAAKANYHIQVEKKSD
                     RDITHSEFKYLTDDERIISIASMLSNDNILDTAIDTARQLLKQV"
     gene            complement(2477..2830)
                     /locus_tag="PKI65_07980"
     CDS             complement(2477..2830)
                     /locus_tag="PKI65_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78409.1"
                     /translation="MNENVSFESEVMYPKSLPNSQCAFILGVLSVFFTVSTIIVGVIL
                     AIFAIIKANSAIKTYRSAPNEFFQSDFHYANTGKILGWVSIGLAIALTIFVIILLIID
                     SLDLISHFVHILKLS"
     gene            3354..5000
                     /gene="hcp"
                     /locus_tag="PKI65_07985"
                     /gene_synonym="priS"
     CDS             3354..5000
                     /gene="hcp"
                     /locus_tag="PKI65_07985"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOB78410.1"
                     /translation="MFCFQCQETAKGIGCSVNGVCGKEDDLARQMDVLIYLCKGLSAL
                     SIELDQKDISNPEVYPFIINSLFQTITNVNFDKAAIKNSIYKGLELKRKLLKLAKNCD
                     VNISKLPDAATWDANTDAEIEAKETEVGVLSTNDEDIRSLRELIVYGVKGICAYAEHA
                     RNLGFEDENIYKFIKKALASTLDNSLSVNDLINLTLQTGEYGVKTMALLDKANTTKYG
                     NPEITKVDLGVRNNPGILISGHDLLDLEELLIQTEGSGIDVYTHGEMLPAHYYPYFKK
                     FKHFAGNYGNAWWKQREEFETFNGPILFTTNCIVPPFEKATYKDRIYTTGSAGLNGAK
                     HIADREYGKMKDFSTIIEHAKRCNPPQAIEQGELIGGFAHNQVIALADKIVDAVNSGK
                     IKKFVVMAGCDGRMKSREYYTEFAKKLPTDTVILTAGCAKYRYNKLNLGDIDGIPRIL
                     DAGQCNDSYSLVVIAMSLKEVFKLNDINELPIIYNIAWYEQKAVTVLLALLYLGVKNI
                     HLGPTLPAFLSSNVTKILVDKFNISSISTVDDDIKMFFAN"
     gene            5018..5344
                     /locus_tag="PKI65_07990"
     CDS             5018..5344
                     /locus_tag="PKI65_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOB78411.1"
                     /translation="MESAFLPEKIYKLKESVVFAEGSVVSKIISKTKAGNVTLFAFDK
                     DQFLSEHTAPFDAIVIVLDGVGQIIINKKDYELSAMEMIILPANVPHAVYGIEKFKML
                     LIMLKN"
BASE COUNT         1966 a          750 c          796 g         1983 t
ORIGIN      
        1 tatatagcct aagtaccgtc aagaaaaagg ctagtgaatt cacgaattgg atatacttca
       61 aatttagggg cgtagctacc aaatatctgc aaaactacat tatgtggtat gtagtgatgg
      121 gtaaatacct cacaggcaag actgtaagca atactgggcg aatgcttaac cttgcatcct
      181 cggacaggtg ggcgtggtat cggtatagag agttagtgaa tataacatat gttaatttaa
      241 acatatatta gttttccgat tgattaattt tcaagtttcc aattcttctc atccaacata
      301 tctcaggtta taatttgtgt aataaaattt tagattgtta caaaaaaaat aactatcttt
      361 aatcatttag gttatttttt tatcacaaag tgctatttgt agccctttta attccattct
      421 tctcagtgtt ctttgtgcat tattagagta ctctgtggtt aatatttaac tgtccaataa
      481 taaatgaact aatattaaca aattactctt ttttaatttt taacccatat aaaacgttat
      541 agaaccgaat ttattaaaac tatcaatgta taaaatagtt gaaagttttt attactaata
      601 tgatatttat aaattagact atttattaaa cttgtttcag taactgtcgt gctgtatcaa
      661 tagctgtgtc tagtatatta tcattgctta gcatagaagc tatagaaata atcctttcat
      721 cgtcagttag atatttgaat tcgctatgtg taatatctct atcacttttt ttctctactt
      781 gtatatggta attggctttg gcagctatct ggggtaagtg tgtaattaca attacttgat
      841 gatgcatcga catatctgat agcaagtctg ccatttgtgc agctatattt cccgaaacac
      901 ctatatctat ttcatcaaag atcagagtag gtaaataatt ttgagaagca atgatagatt
      961 ttagtgctag cattaatcta ctcatttctc caccacttgc cactctttct aatgcttctg
     1021 gttctatccc cttattagca ctaaatttta gttcaagttt atcaaacccg aaaattgttg
     1081 gttcatctaa tttttctaat tgtattataa atttaccatc ttgcatacct aatctgctga
     1141 taagttttat tattagcttt tctaactcag gaattgcttt tttgcgttca ttatataatt
     1201 ttattgcagt atctcttagt tctgtttcta tttgcaaaag attggcttct ttttttacga
     1261 ttttatttgc taactcatct atgtttttaa tatccttttt taaattttct tgtagctcta
     1321 gcaactcttc agtagtctgc acatgatgtt taaacattaa cttttgaaat aaatctaatc
     1381 tattctctat tctttgtaat tcatcaggag aataatctaa agaatcagct agttctgaaa
     1441 gtgacattga tatatctttt atttctattt ctatattttc taaaatatca ataaactttt
     1501 cagcttttgg taggtaagaa ctaatatttc tcaatttttg tattatttga gcaatattct
     1561 gataaatatt ttgttcattt tcataaagta gagctgtggt ctctttaata gtttgcaaag
     1621 tcaattcaga atgcgatagt aattcatatt gctgatgcaa ctcttcatat tcattttctt
     1681 taatatctgc attttttaat tcttcccatt gaaattgttt ataatcattc tcatgcatca
     1741 gttgtcgcat catttctcta tcttcttcaa tttctttttt aaaaaatgta aaattttcgt
     1801 ataattttaa gtatttagta aataaatcat tcgtattcgc aaaagaatct aaaagtgtgt
     1861 attgaaattt ttttgtacct aaaagaagcg tatcaaattg agaatgaata ttaatgagtt
     1921 gctctccaat gctttttaaa atggataaat ttacaggagt atcatttata aatgcacgag
     1981 atcgcccatt aggagaaatt tctctgcgaa ttatcaatgt atcttcatat tctaaatcat
     2041 tttgctcaaa aaaatgttta aggtccaatt gtttgatatc aaatacacct tctatcacac
     2101 attttttgct tttatctagt aaagcccctg aatcagctct ttcacctaaa agtagctgaa
     2161 tagcacccaa aagaatactt ttgccagagc ccgtctcacc agtgatcact gtaaaaccat
     2221 tataaaaatc taatgacaaa ttgtgaataa gcaaataatt ttttatatct agatgttgta
     2281 acatttctga tcttaatgtg tttatacaaa gttaattgaa aaatatatta taaaccaata
     2341 aaaattttta taaaaccata ttataagtta tgttcaaccc tattagttta catattttct
     2401 tataaaatta gtaaatgtaa tctataaata aaatgctttt gataatagta aaaactgaca
     2461 taacattttt tgacacttac gacaacttca aaatatgcac aaaatgcgat atgagatcta
     2521 aagaatcaat aattaatagg attattacaa aaatagtaag tgcaattgca agtccgatac
     2581 tcacccaacc aagtatttta ccagtattag cataatgaaa atcggattga aaaaattcat
     2641 ttggtgctga tcgatatgtt ttaatggcac tattagcttt tataatagca aaaattgcta
     2701 gtattactcc tacaataata gtagatacag taaaaaatac tgataaaacg cctaaaataa
     2761 aagcacattg tgaatttggt aatgattttg gatacattac ttctgattca aaagatacat
     2821 tttcattcat aatatttatt ttttttaaaa ttagcacaca aacttaataa taaatattta
     2881 aatatataat ttattagtta caaaaataaa tagattagat ttattaatat taataaaaga
     2941 cttaaaaaat aggctagcat gagatatata tagatgatat aacccataat aacaacatta
     3001 ttttattttt gaattattat tttttatatt atgttaaaac gagtaagtat acgtaatatc
     3061 atagttttgt atttaataga tagagagttt attaattttt ttttaaaaaa aatcctatga
     3121 atgactaaat gcctcaagat tatatatatt ttaatatttt atcgtttacc taaatagaag
     3181 ttaatgttac attttatcaa aatataaatt tagattatta ttacctaatt gtgtaataat
     3241 aaattatacc tgatatatat cataatttac taattaacct tttaattata ttaattttga
     3301 cattgaaatt ataataattg aaacttaacc cccaaaaaat aaaaattatg aatatgtttt
     3361 gtttccaatg tcaagaaaca gccaaaggta taggctgtag tgtaaatggt gtatgtggta
     3421 aagaagatga tttggctaga caaatggacg ttttgatata tctatgcaag ggattatctg
     3481 cactatctat agaattagat caaaaagata tatctaatcc ggaggtctat ccttttatta
     3541 taaatagcct atttcaaact atcacaaatg taaattttga taaagcagct atcaaaaata
     3601 gtatttacaa aggtttggaa ctaaaaagga aattattaaa attagccaaa aattgtgatg
     3661 tgaatattag taaattacct gatgccgcta cttgggatgc taatactgat gcagaaatag
     3721 aagctaaaga aacagaagta ggtgtattat ctacaaatga tgaagatata cgctcactga
     3781 gagagttaat agtgtatgga gttaaaggta tatgtgctta tgcagaacat gctcgaaatt
     3841 taggcttcga agatgaaaat atttataaat ttatcaaaaa agctttagct tctacacttg
     3901 ataattcatt atcagtaaat gatttaataa atttaacatt gcaaactggc gaatatggtg
     3961 tcaaaactat ggcattgcta gataaagcaa atactactaa atatggtaat cctgaaatta
     4021 ctaaagtaga tcttggagtt agaaataacc ctggcatctt aatatcaggt catgatttgc
     4081 tggaccttga ggaactatta atacaaactg aaggcagtgg tatagatgtt tacacgcatg
     4141 gagaaatgct tcctgcccat tattatccct attttaagaa atttaaacat tttgcaggca
     4201 attatggtaa tgcatggtgg aaacaacgtg aagaattcga aactttcaat ggaccaatac
     4261 ttttcactac taattgtata gtgccaccct tcgaaaaagc tacttacaaa gatagaatat
     4321 acactactgg ctctgcaggt ttaaatggtg ctaaacatat agctgataga gaatatggta
     4381 aaatgaaaga tttttctact ataatagaac atgctaaacg ttgcaatcca cctcaagcta
     4441 tagaacaagg agaactcata ggtggctttg ctcacaatca agtaatagca ttagcagata
     4501 aaatagtaga tgctgtaaat tctggtaaaa tcaaaaaatt tgttgttatg gccggttgtg
     4561 atggtagaat gaaaagcaga gaatattata ctgaatttgc taagaagcta cctactgata
     4621 cggtgatatt gacagcagga tgtgctaaat atcgttacaa taaattgaat ctgggcgaca
     4681 tagatggtat accacgaata ttagacgcag gacaatgtaa tgattcatat tctctagttg
     4741 tcattgctat gagtcttaaa gaagtcttta aactcaatga tataaatgaa ttgcctatta
     4801 tttataatat tgcttggtat gagcaaaaag cagttactgt attgctggca ttattgtatc
     4861 taggtgttaa aaatattcac ctcggtccta ctttaccggc ttttctttct tctaacgtaa
     4921 caaaaatatt ggtagataaa ttcaatatta gtagtattag cactgttgat gatgatataa
     4981 aaatgttttt tgctaattaa tgaaaaaatt attaaaaatg gaatcagcat ttttaccaga
     5041 aaaaatatat aaattaaaag aaagcgtagt ttttgccgag ggatcagtcg taagcaaaat
     5101 aattagtaaa actaaagcgg gcaatgtaac gctatttgct tttgataaag atcaatttct
     5161 aagtgaacat acagctccat ttgatgcaat tgtaatagtt ttagatggtg ttggtcaaat
     5221 aattattaat aaaaaagact atgaattgag tgctatggaa atgatcatat tgcctgctaa
     5281 tgtgccacac gccgtatatg gtatagaaaa gtttaaaatg cttttgataa tgctaaaaaa
     5341 ctaacattta aaacaaaggt aattgaataa tcggaattaa taaatgttta agtttttcaa
     5401 gtttatatgt taaataattt ttatattttt acaaaaatta ttattcatga aaaaaatagt
     5461 ggaaaacacc agtaaatacc ttgctatgag taagg
//
LOCUS       DAOPRN010000094         5434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_12249_length_5434_cov_123.905559, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000094 DAOPRN010000000
VERSION     DAOPRN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12249_length_5434_cov_123.905559"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKI65_07995"
     CDS             <1..578
                     /locus_tag="PKI65_07995"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78412.1"
                     /translation="NINFFGVVEMDELLMDYSEKGRIYKNAEELKRARKQKPKKVAVL
                     AATDRAGNLLFKKFESKKLSSEQIEQFLKAKMPKDGVMCSSNKKAFKKVRRNVIKHKE
                     IVTNHRQKRGIYSLSTVKKKTSEFTNWIYFKFRGVATKYLQNYTMWYVVMGKYLTGKT
                     VSNTGRMLNLASSDRWAWYRYRELVNITYVN"
     gene            956..2443
                     /gene="amrB"
                     /locus_tag="PKI65_08000"
     CDS             956..2443
                     /gene="amrB"
                     /locus_tag="PKI65_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOB78413.1"
                     /translation="MKKKFIIICFMVASLFSNCQYPIRKPIVQGTFYPASATELKKEI
                     ESWYKAYQPTKYITPIRAIIVPHAGYVYSGAIAARAYAQIEPGAKYKNVFLIGRSHQG
                     YFSGASLYTSGAFANALGEVEVNDDICKKLLQHKIFDHPSYYHDNEHSLEVQLPFLQL
                     RLAPDFKIVPILIGTENTIILDEIATILKQYYEPENLFVISTDLSHYPSYKDAIISDS
                     LTVEAILSGDPKKFQTTVNYIETSGKYPNLSTAACGAAAVYVLLKIIENEPCAIKKIY
                     TANSGDISGDHSRVVGYASLIVVPSDKNTNIQTKSTDTEFTLTDEEKKFLKDLAFKSI
                     KYGLTYHSFPKVDIPDWPGLHTNCGVFVTINKRGKLRGCIGTFRQDRPLWQNVMEMAQ
                     SAAFSDPRFPPLSFDELNEIDIEISVLTPLKKINSIDEIQLGKHGIYIKKGMNSGTFL
                     PQVATDTGWTLEEFLGHCARDKAGIGYYGWKDPDAEIYIYEAIVF"
     gene            complement(2440..3132)
                     /locus_tag="PKI65_08005"
     CDS             complement(2440..3132)
                     /locus_tag="PKI65_08005"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOB78414.1"
                     /translation="MNDELFHIVNKRTFLIALLEATFIVILPIWGYILKDIPGVNEFL
                     IGVIPRDLQHIYGIFIFPLVHNDLSHLFGNVIALYVLLLLLKNSFSPYYYRIIACGWV
                     FPGIFIWLIGNSNTMHIGASGLVYHLAFFIFWIGVVIRHPNFIAQSLIVIFLYGGFFW
                     GILPLFPEVSWEGHLGGFISGTLQALILYKPISMLYPKQEEEEDEDDAPPDWYEEEND
                     NIIDFRDTKKNN"
     gene            3463..5250
                     /gene="dnaX"
                     /locus_tag="PKI65_08010"
     CDS             3463..5250
                     /gene="dnaX"
                     /locus_tag="PKI65_08010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOB78415.1"
                     /translation="MKTQQYLVSARKYRPTRFDEVVGQEVIVKTLKNAIKTDQLGQAF
                     LFTGPRGIGKTTTARIFAKTINCENITPDGEACNECPSCISFNQNNSYNIYELDAASN
                     NKVDDIRALIDQVNIPPQVGKYKVYIIDEVHMLSDSAFNAFLKTLEEPPAYAKFILAT
                     TERHKILPTILSRCQIYNFKRISNEDIVKHIEKIAKLENISYEVEALHVIAENSDGAL
                     RDALSMFDQLASFGEGKISYHIAIENLNIVDYDLYFQITDTLIKADISKTLLLVDQII
                     ANGFEPIHIIKGLAEHTRNLLFTSMNATVELLEISETHKQKLISQVKYCSYDFLSNSL
                     KILSEADVAYMNTHNKRLLLEVSLLKIIDNNKKNNDEKFTDLSSDTLNINTKKSTVAT
                     KENIQRNDFTSVNSTKHTTPTKLANGINSSGIVNTFSIREKIKEYHTKKENKKELNEK
                     NIKELLNQLANEIINDKPSLSHILSKINIDFDGQKLTFNVENLIEAESINKNLIDIKQ
                     YLANNLIQNGYEIQIKVNESTQKEKYKKEKSKIELNKKANNNTTALNKKASNNEDDEL
                     FFDELKNKYPSLNILAKKYNLKPTNPQNI"
BASE COUNT         2145 a          843 c          790 g         1656 t
ORIGIN      
        1 ataatatcaa ttttttcggg gtagtagaga tggatgaact attgatggat tattctgaaa
       61 aaggcagaat atacaaaaat gccgaagagc tgaagcgggc taggaagcaa aagccaaaga
      121 aagtagccgt attagctgct acagacagag ctggcaattt attattcaaa aaatttgaaa
      181 gcaaaaagct aagttcagaa cagatagagc aatttctgaa ggctaagatg ccaaaggatg
      241 gagttatgtg tagcagtaat aaaaaagcat tcaaaaaggt tagaagaaat gtaatcaagc
      301 ataaagaaat agtaactaac catagacaaa agcgaggtat atatagcctg agtactgtca
      361 agaaaaaaac tagtgaattc acaaattgga tatatttcaa atttagagga gtagctacca
      421 aatacctgca aaactacact atgtggtatg tagtgatggg taaatacctc acaggcaaga
      481 ctgtaagcaa tactgggcga atgctcaacc ttgcatcctc ggacaggtgg gcgtggtatc
      541 ggtatagaga gttagtaaat ataacatatg ttaattgaaa catatattag ttttccgact
      601 gattagtttt caagttttca attctccccg tccaacatat ctcaggttat aatttgggta
      661 atcaaatttt aaattgttac aaaaaaatca ttatttttta attatttagg ttattttttt
      721 accacaaagt cccatttgta gcccttttaa ttccatcctt ttctgtgttc tttgaacatt
      781 attagagtac tctgtggtta atatttaact gtccaataaa agataaacca atattaacaa
      841 attactcttt tttttaattt ttaacccata taaaatgtta tagaacctaa attttacata
      901 aaaaaatttt ataattttta ttttttatat gaaaaaaaat atataaattt gtagtatgaa
      961 aaaaaaattt ataattattt gttttatggt agctagttta ttttcaaatt gtcagtatcc
     1021 tatacgtaaa cctatagtgc aaggcacatt ttatccagct agtgcaacgg aattaaaaaa
     1081 agaaatcgaa agttggtata aagcatatca acctacgaaa tacataactc caatcagagc
     1141 aataatagta cctcatgctg gatatgtcta tagcggagct atagccgcta gagcatatgc
     1201 acaaatagag cctggagcta aatacaaaaa cgtattttta ataggcagaa gtcatcaagg
     1261 gtattttagt ggtgcatcgc tatatacatc tggtgctttt gctaatgctc tcggagaagt
     1321 agaagtaaat gatgatatct gcaaaaaatt attgcaacac aaaatattcg atcatccaag
     1381 ctattatcac gacaatgagc attcattaga agtacagctt ccttttttgc aattacgctt
     1441 agcaccagat tttaaaatag ttcctatctt aataggtacc gaaaatacta taatcttaga
     1501 tgagatagct accatactaa aacaatatta tgaaccagaa aatctttttg taatcagtac
     1561 agatctatct cattatccat catacaaaga tgctattatt tctgatagtt taactgtaga
     1621 ggctatcttg tctggtgacc cgaaaaaatt tcaaactaca gtaaactaca tagaaacatc
     1681 gggtaaatat ccaaatttat caacagcagc atgcggtgct gctgctgtat atgtactttt
     1741 aaaaataata gaaaatgaac catgcgcaat taaaaaaatc tatactgcta atagtggcga
     1801 tatatctggt gatcactcga gggtggtagg atacgctagc ctaatagtag taccatctga
     1861 taaaaataca aatattcaaa ctaaatctac tgatactgaa tttactctaa ctgatgaaga
     1921 gaaaaaattt ctcaaagatt tagcctttaa aagcattaag tatggattaa cgtatcatag
     1981 ttttcctaag gttgatattc ccgattggcc aggattacat actaattgtg gtgttttcgt
     2041 tactataaat aaaagaggaa aactgcgcgg ttgtataggg acatttagac aagacagacc
     2101 attatggcaa aatgtgatgg aaatggctca gagtgcagca ttcagtgatc ctcgtttccc
     2161 gccacttagt tttgatgaac taaatgaaat agatatagaa atcagtgttt tgactccatt
     2221 gaaaaaaatt aattctatcg acgaaataca attaggcaaa cacggtattt atatcaaaaa
     2281 agggatgaat tctggtacct tcctgccaca agtagctacc gatactggtt ggacattaga
     2341 agaatttctt ggtcattgtg ctcgagacaa agctggcatc ggatattatg gatggaaaga
     2401 tcctgatgca gaaatatata tttatgaagc tatagtcttc taattatttt ttttagtatc
     2461 tctaaaatct ataatattat cattttcctc ctcgtaccaa tcaggcggag catcgtcttc
     2521 atcttcctcc tcctcttgtt taggatacaa cattgatatt ggtttataca aaatcaatgc
     2581 ttgcaaagta ccagatatga aacctccgag atgtccttcc caagaaacct caggaaataa
     2641 aggaagaatt ccccaaaaaa aaccaccata tagaaaaata actatcaaag attgtgctat
     2701 gaaattggga tgcctgatca caacaccaat ccagaaaata aaaaaagcca aatgataaac
     2761 taatccacta gctcctatat gcatagtatt actattacct atcaaccaaa taaatattcc
     2821 aggaaaaacc catccacatg ctattattct gtaataataa ggcgagaaag aatttttcaa
     2881 aagcaacagc aatacatata aagcaatgac attaccaaat aaatgactca aatcattatg
     2941 aactaaagga aaaataaaaa taccatatat atgttgcaaa tctctcggta tcactcctat
     3001 caaaaattca ttaacacctg gaatatcttt tagaatatag ccccagattg gtaaaataac
     3061 tataaaggtg gcctctaata aagcaattaa aaaagtcctt ttatttacta tatgaaaaag
     3121 ttcatcattc atacacttta ataaattatt ttgtaaattt tatttattaa aaataatttg
     3181 tctataaaat tataaaatta ttccttttat atgatatgac aataatttat aaaccactta
     3241 aaatatttaa taataattat ttttaagcta taactaacaa tgatgatata tcaatgtgga
     3301 atgaaaaaaa gcaaaataat tatgttttta atctatttgt ttgctaaaac aacataaata
     3361 acatatattt taatctagta caaaaatatt gcttcacttg taaattccta aaaagataaa
     3421 aaatgttttt tattttattt ttattttatt acttttgcaa atatgaaaac tcaacagtat
     3481 ttagtttctg cacgtaaata tagaccaacg agatttgacg aagtagttgg gcaagaagtg
     3541 atagtaaaaa ccctaaaaaa tgctatcaaa actgatcaac tagggcaagc tttcttattt
     3601 accggccctc gaggtatagg caagactact acagcacgca ttttcgctaa aactattaat
     3661 tgtgaaaata tcactccaga tggtgaagct tgtaatgaat gcccatcatg catatctttt
     3721 aatcaaaata actcgtataa tatttatgaa ttagatgcag cttctaataa caaagtagat
     3781 gacataagag cactaataga tcaagtaaat ataccaccac aagttggcaa atataaagtt
     3841 tatataattg acgaggtgca catgctatct gatagtgctt ttaatgcttt tttaaaaact
     3901 ctagaagagc ctccagccta tgctaaattt attttggcta ctaccgaacg acataagatt
     3961 ttacccacta ttctgagtcg ttgtcaaata tataatttca aacgtatatc aaatgaagat
     4021 atcgtaaaac acattgaaaa aatcgcaaaa ttagaaaaca tatcttacga agtagaagct
     4081 ctacacgtga tagccgaaaa tagtgatgga gccttgcgag acgcattgag tatgtttgat
     4141 cagcttgctt catttggcga aggcaaaata tcatatcata tagctataga aaacttaaat
     4201 atcgtagact atgatcttta ttttcagatt actgatacgc ttataaaagc tgatatatct
     4261 aaaacactat tactcgtaga tcagatcatt gctaatggat tcgaaccaat tcatatcata
     4321 aaaggattag ctgaacatac tcgaaattta cttttcactt ctatgaatgc tacagttgag
     4381 cttttagaaa taagtgaaac tcataagcaa aaattaataa gtcaagtaaa atattgttcc
     4441 tatgattttc tctcaaattc tctaaaaatt ttatctgaag ctgatgtagc ctatatgaat
     4501 actcacaaca aaagactcct actcgaggtt tctcttttaa aaattataga taataataaa
     4561 aaaaataatg acgaaaaatt tactgattta tcatcagata cattaaatat aaataccaaa
     4621 aaatctacag ttgctaccaa agaaaatata caacgaaatg atttcacatc tgtcaatagt
     4681 acaaaacaca ctactcccac taaattagct aatggcatta attcgtctgg tattgtaaat
     4741 acatttagta taagagaaaa aatcaaagaa taccatacta aaaaagagaa caaaaaagaa
     4801 ttaaatgaaa aaaatattaa ggaacttcta aatcaactag ctaacgaaat aataaatgat
     4861 aaacctagcc tttcacatat tttatctaaa ataaatattg attttgatgg acaaaagctc
     4921 acattcaatg tagaaaatct aatagaggct gagagtatca ataaaaattt aatagatatc
     4981 aagcaatatc ttgctaataa tttaattcaa aatggttacg aaatacaaat aaaagtaaat
     5041 gaatctacac aaaaagaaaa atataagaaa gaaaaaagca aaatagaatt aaataaaaaa
     5101 gcaaataaca atacaacagc actaaataaa aaagcatcaa ataacgaaga tgatgaatta
     5161 tttttcgatg aattaaaaaa taaataccct tcattaaata ttttagctaa aaaatataat
     5221 ttgaaaccta caaatccaca aaatatataa aaattatgag aataaaaaaa ttaaaaattt
     5281 tattaacttt aataattgca ctgggaatag ggcaattatt tgctcaagtt agcaattatt
     5341 catacacaac tgtccctaat gacccaatga acacccgaat atatacctta gataatgggt
     5401 taaaagtttt catgactgta tacaaggcag agcc
//
LOCUS       DAOPRN010000095         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_12837_length_5228_cov_118.778852, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000095 DAOPRN010000000
VERSION     DAOPRN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12837_length_5228_cov_118.778852"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1059
                     /locus_tag="PKI65_08015"
     CDS             7..1059
                     /locus_tag="PKI65_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOB78416.1"
                     /translation="MVAAIASGKAPDLVNLNAQYVLDYVAKNALQPLDEYVTEVDKFL
                     YWEGLWNGTVIDNKAYAITWYVGPSGVMIYNKQLFEKAGLDPNKPPETWEEAIEYSKI
                     IKEKTGAYGIQPNPGGINNLSDEGIQLVSPDGKKATFNTPEAVKKLESMQQAFKEGLF
                     PPDFIIGDWQTGTQKYSAGELAMFPASTMIKHIQINSPSIYAVSDIAPSPRKKSGVTR
                     LDPMNLAVPYNAKNKEEAVKFGLWLTSPYWQIEFSKLATIVPSTKVTLETDLWFVERA
                     EEDLNIKAQMMSNSSKLMMYAEDSTAIGKIIGASKYSEFKRILDEYWQAGIKGEYTAQ
                     EALSLAEKECNKLLAE"
     gene            1147..2028
                     /locus_tag="PKI65_08020"
     CDS             1147..2028
                     /locus_tag="PKI65_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOB78417.1"
                     /translation="MKLTRKKQQLVVIIAFLLVPIILLGVFSYYPIIKGTILSFADYN
                     MLTSEINWVGLKNYRWLFNYKYFYVSLLNTLKYLIVVPFIQFASMGLAVLVNQQVPGI
                     KIFRTLFYVPVITGSVIVSIAWRWIFDVDGILNYFLMSLHLIKEPIMWLLDKNMALFS
                     CMFVTFWRGLGYYMIIYLAGLQNIPSELYEAASLDGANSFKKFTKITVPLLKPTMLLC
                     FILSSMSALQVFEEILLLTEGKSQTTTLMFELYDLAFNKLEFGKSAALAIVLSIFLIC
                     LTFLNFKIFGLGGEKEK"
     gene            2025..2861
                     /locus_tag="PKI65_08025"
     CDS             2025..2861
                     /locus_tag="PKI65_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOB78418.1"
                     /translation="MKSSLRKKRIISLTIKYIILILLVFFFLFPFLWALSTSLKTATD
                     VFKWPPEWIPKPISFESYKYVLSELPIFKYFINSMIITILGVLFNVVLSSMAAYPLAR
                     LEFKGRNLIFFLIILPMMVPMQSTLIVNFITILHLNLFNSYISVVLPTAVSSFGIFLM
                     RQNYLSIPRELEDSAKIDGCNEFQIWSKIAFPLVKPAAGALAIFSFVAYWNSFLWPLI
                     VLKDPNKYPIQVGIAYLNNMFAQNYRHICASLIIAVIPILIFFFFTQRYFIEGYQGAL
                     KG"
     gene            3208..5214
                     /locus_tag="PKI65_08030"
     CDS             3208..5214
                     /locus_tag="PKI65_08030"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOB78419.1"
                     /translation="MKHNTLIPKISIQETNNGIKRILVDYNSFWIEDHISYISVIYSV
                     TIKSDSCETIWNIEVKNNETNYLIEEVIFPLISGVYLGKSWEDDVLVFPFNAGEKINN
                     PIEAYEKGSSTIGWKWQEYKYLYSIDGIETQKDLDGFFYRQCDYSGPLSMMWLDYFDS
                     EGGLYLSCYDDDFMIGSLRAETLGKKRPGMGFSIIKYPQIKKGDTWKSPSYGLAIHDE
                     DWHWASDSYRKWRNSCKSVKLSIPEWFKRSTALVAHYDFKYQNGDIVHKFKDIPKIYK
                     QAIKMGVDHILISGWHKDGFDNGFPQYTPDPDLGTEEDLKQAVRFIRNLGGHVTFYIN
                     SQLCNIKYEDKKELIEECSVINKKREIETTSYGDESITFSVMCSNADKWKEYLINVVD
                     YLVNIIGVDGIYFDQLGMAKPQFCYNEKHNHHIADWNKGYRNLILDCLHIFQNERGND
                     PILFLYEGVTDIHGEYISAQLISTFFYYHCGAYPELYKYTFPEQILVDMVYPRKNLSM
                     RPVHIAQVSREMINKAFILGSYFWIYDLEEDNTFKNDPEQYEYLKKVIDLRKVWLNLY
                     GQGIFKDTVGIEVNDNGIIAKLYYLNDNSSLITIANASMKKEGKVKFYCDPSKVKIVT
                     CNYLNEPNYPIELVWIPKEESDKKYIEIVIPNYELALIHIEFYE"
BASE COUNT         1881 a          641 c          921 g         1785 t
ORIGIN      
        1 cagaagttgg tagcagctat agcttcagga aaagcaccag atttggttaa tttgaatgct
       61 cagtatgtct tagattatgt agctaaaaat gctctacaac ctttagatga gtacgtaacg
      121 gaagtagata agttccttta ctgggaaggg ttgtggaacg gaactgtaat agataacaag
      181 gcatatgcta ttacttggta tgtaggtcca tctggagtaa tgatttataa taaacagctt
      241 tttgaaaaag ctgggttaga tccaaataaa ccgccagaaa cttgggaaga agcaatagaa
      301 tattcaaaaa taataaagga aaaaactgga gcctatggaa ttcaacctaa tcctggagga
      361 attaacaact tatcagatga aggtatacag cttgtaagtc ctgatgggaa aaaagctact
      421 tttaatactc cagaagctgt taaaaagtta gaatcgatgc aacaagcttt taaagaaggg
      481 ctttttccac cagattttat tataggtgat tggcagacag gaactcaaaa atattctgcg
      541 ggtgaattgg ctatgtttcc agcttcaact atgattaaac atattcaaat taatagtcca
      601 agtatttatg cagtgagtga tatagctcca agtcctagga aaaaaagtgg ggttactcgt
      661 ttagatccaa tgaacttagc tgttccttac aatgcaaaaa ataaagaaga agctgtaaaa
      721 tttgggttat ggctaacttc tccatattgg caaatagaat tttcaaaatt ggcaactata
      781 gtcccttcga caaaggtcac tttggaaacg gatctttggt ttgtcgaaag agcagaagaa
      841 gatttaaata ttaaagcgca gatgatgagt aatagtagta agttaatgat gtatgcagaa
      901 gattcaactg cgattggaaa aatcataggt gcatctaaat actctgaatt caaaagaata
      961 ttagatgaat attggcaagc tggtataaaa ggcgaatata ctgcccaaga agctctttct
     1021 ttagcagaaa aagaatgtaa taagttattg gctgaatgaa attagaaaaa ttaaagtgat
     1081 aggtgttaat ctcacaccta tcactttata aatttgaata tcgtgccatg atcaggaggt
     1141 gtcttaatga aattaactcg aaaaaagcaa caattagttg taattattgc ttttttattg
     1201 gtaccaataa ttttgctagg tgtttttagt tactatccaa ttattaaagg aactatatta
     1261 tcgtttgcag actacaatat gcttacaagt gaaataaatt gggttggatt aaaaaattat
     1321 cgctggcttt ttaactacaa atatttttat gtgtctttat taaatacttt aaagtattta
     1381 atagtggttc ctttcattca atttgcttct atgggacttg cagttttggt aaatcaacaa
     1441 gttccaggaa taaaaatatt taggactcta ttttatgttc cagtaattac aggtagtgtt
     1501 attgtaagta tagcttggag atggattttt gatgtagatg gaattttgaa ttattttcta
     1561 atgtctttac atctaataaa agagccaata atgtggcttt tggataaaaa tatggcgttg
     1621 ttttcatgta tgttcgtaac tttttggcgt ggtcttggtt attatatgat tatttattta
     1681 gcaggtttgc aaaatattcc ttctgaacta tatgaggcag cttctctaga tggagcaaat
     1741 agttttaaaa agtttacgaa gataaccgtt cctttactta aacccactat gcttttatgt
     1801 tttattttat cttcgatgtc ggcacttcaa gtctttgagg agattttatt gttaactgaa
     1861 ggaaagtcac aaaccactac gttaatgttt gaattatatg acttagcttt taacaagcta
     1921 gaatttggaa aatctgcagc gttagcaatt gtgctttcaa tttttttaat atgtttaaca
     1981 tttttgaatt ttaaaatatt tggcttagga ggcgaaaaag aaaaatgaaa agctcattaa
     2041 gaaaaaaaag aatcatatca ttaacgataa aatatataat tttaatttta ttagtatttt
     2101 ttttcctatt tccttttttg tgggcgttat ctacttcttt aaaaactgca acggatgtat
     2161 ttaaatggcc tccagaatgg atacccaagc cgattagctt cgagtcatac aaatatgttt
     2221 taagcgaact tcctatattt aaatatttta ttaattcaat gataattact atattaggag
     2281 tactttttaa tgtagtttta tcttcgatgg ctgcctaccc attagcaaga cttgaattta
     2341 aaggtagaaa tttaattttt ttcttgatta tattacctat gatggttcct atgcaaagta
     2401 ctttaattgt gaactttatt acaattttgc atctaaattt gtttaattcc tatatatcag
     2461 tggtattacc gacagctgtt agcagttttg ggattttcct aatgagacaa aattatttgt
     2521 caattccacg cgaattagaa gattctgcga aaatagatgg ttgtaatgaa ttccaaattt
     2581 ggagcaaaat tgcttttcca ttagtaaaac cagcagcagg agctttagct atattttctt
     2641 ttgtggcata ttggaattcg tttttatggc cattaattgt tttaaaagat ccaaataaat
     2701 atcctatcca ggttgggatt gcttatctta acaatatgtt tgctcaaaat tacagacata
     2761 tttgtgctag tttaataata gcagttattc ctatattaat atttttcttt ttcactcaaa
     2821 gatattttat agaaggctat caaggggcct taaaaggatg aaatacaatt catttgtgtt
     2881 tattaatata ttaataaaaa taaatactaa ttaattacct ttattgattt ttaaaaaaac
     2941 attatttaca gtcttttact aattatgaaa aatactttta tggaggtcaa tgattaaatg
     3001 cttaaatcaa agattaaatt tggcaatcaa tttttaagta tgacatttga tggtttaagt
     3061 ggcgaactat tagaacttgt caacatgaaa aatagtgata atctgctaaa aaattggaat
     3121 cctctaagga ttatgccttt ttctataaaa ttaaaaacta agaatggaag taatgtggtg
     3181 ttacaacctc caaatcgtaa gaatgttatg aaacataata ctcttatacc taaaatttct
     3241 attcaagaaa caaacaatgg tattaagcgt attttggttg attataatag tttttggata
     3301 gaagatcata ttagttatat atctgtaatt tattctgtaa caatcaaatc agatagttgt
     3361 gaaactattt ggaacataga agtaaagaac aatgaaacga attatctaat agaagaagtg
     3421 attttcccgc ttatatctgg ggtttatctt gggaaaagtt gggaagatga cgttttagtg
     3481 tttccattta atgctggaga aaagataaat aatccaattg aagcttatga aaaagggtca
     3541 agtactattg gatggaaatg gcaagaatat aaatatcttt actcaataga tgggatagaa
     3601 actcaaaaag atttagatgg ttttttttat agacaatgtg attattcagg accgttatca
     3661 atgatgtggc ttgactattt tgatagtgag ggagggttat atctttcatg ttacgatgat
     3721 gattttatga tcggaagtct aagagcggaa accttgggta aaaaaagacc tggcatggga
     3781 ttttcaataa taaaataccc tcaaataaaa aaaggagata cttggaaatc tcctagttat
     3841 ggtttggcga tacatgatga agattggcat tgggcttcag atagttatag aaaatggaga
     3901 aattcttgta aaagtgtaaa attatcaatt ccagaatggt ttaagcgtag cacagcctta
     3961 gttgcgcatt atgattttaa atatcaaaat ggtgatattg tccataagtt taaagacatt
     4021 ccaaaaatat ataaacaagc tattaaaatg ggggtagatc atattttaat atcgggttgg
     4081 cacaaagatg gatttgataa tggttttcca caatacaccc cagatccaga tttaggaaca
     4141 gaggaggatc tcaaacaagc agtcagattt ataagaaatt taggaggtca tgtaacattt
     4201 tatataaaca gtcaactatg taatattaaa tatgaagata aaaaagaact tattgaagaa
     4261 tgctctgtaa taaataaaaa aagagaaatt gaaaccacaa gttatggcga cgagtcaata
     4321 actttcagcg taatgtgtag taatgctgat aaatggaaag aatatctaat aaatgttgtg
     4381 gattaccttg tgaatattat aggagtagat ggtatctatt ttgatcaact tggaatggct
     4441 aaaccacaat tttgttataa tgagaaacat aaccatcata ttgctgattg gaataaagga
     4501 tatagaaatt taatattaga ttgtttacat atatttcaaa atgaaagagg caacgatcct
     4561 attttatttt tgtatgaagg agtaactgat attcatggtg aatatatctc agcccaactt
     4621 atatcaactt ttttctatta tcattgtgga gcatatccag aactttataa atatactttt
     4681 ccagaacaga ttttagtcga tatggtttat ccaagaaaaa atttatctat gcgtccagta
     4741 catattgcgc aagtatctag agaaatgata aataaagctt ttatcttagg atcatatttt
     4801 tggatttatg atcttgaaga agataacaca tttaaaaatg atccggaaca atacgaatat
     4861 ttaaaaaaag taatagatct aagaaaagtg tggttgaatt tgtatggaca aggtatattt
     4921 aaagatacag taggaattga agtaaatgat aatggtatta tagcaaaatt atattattta
     4981 aatgataatt caagtcttat aacaattgca aatgcatcta tgaaaaaaga aggaaaagtt
     5041 aaattttatt gtgacccttc aaaagtaaaa atagtaactt gtaattattt aaatgagcct
     5101 aattatccaa ttgaattggt ttggatacca aaagaagaaa gtgataagaa atacatagaa
     5161 atagtaatac ctaactatga gttagctttg atacatatag aattctatga ataaaatgaa
     5221 tgtatatt
//
LOCUS       DAOPRN010000096         4774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_14449_length_4774_cov_103.675355, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000096 DAOPRN010000000
VERSION     DAOPRN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14449_length_4774_cov_103.675355"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..3652
                     /locus_tag="PKI65_08035"
     CDS             467..3652
                     /locus_tag="PKI65_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOB78420.1"
                     /translation="MNKDIKYIIISLLLLFLGVLLYSQSIVRWGLPYVKNYYPYEYNG
                     YSQNWDICFDSTGVLWLANGDGLISFDGLNWRKYFLPSYDAVACISIINNRIYIGSKN
                     EFGYFNISSPSEYKYVSLTNKAMNLSLSNNYFFYSAKTDYGVYMSDGNSVVRIHNDNV
                     DVLFTNVKGNLISFNNLAFFYNSATGLYLLSGSKSLYIKGSYPLNSNISMFLPYTDDT
                     LLIIDNKTGIYKGCLNLQNIINPSLTIEEFDFPNEKELIANVILDARKTSFGYIFSTY
                     FGGTYVYDLDFKLQYILNQESQLISITHNKIALDPNENIWLAMDYGIARINTHNPFTL
                     LSKVHGLSSSILDVEFFNNKLLAGGWTNLYIEELDKSTNYFKKTELENICWQLKKINF
                     GIDSFVIVASSDGLFAINNKNKLTKIDTGNFRTVNITHSPTYITATTDNLFNVYHYKN
                     NKFIKDLSLPLPLYNTFSNINIKISDSSYVHYISCDEPKIIKITISETIQMMQVITLP
                     IKQNSPTGYLLEKIDNDILIYSTENIWLLKSNNDKITLAAPELRKYYDSIHYINILRV
                     FGDTIWLQRSSKLLPNKPIYLLVKEKGRYVHKKTLQLFHELEILSIKNTPYDNILFAT
                     DDGIFVLNQKKLFTKTTPLKLYASLDILAKDSTYLFKIFSGLDTVLKLPYPLNKLKIE
                     WRTNLIERELTHNIKFSYYLEGYENEWSEWSTLHIKEYNDLKPGNYTFHIKAIDENEQ
                     AKSIISIPIHINVPFYRRVVSYIIYAIAGIFIIYSIVYLSNRLIIRRNKLLEEYAIAK
                     NKENLQKTLELEKEKEKADRLLINILPVRIAEELKEKGKVDAEFFSSVSVIFTDFTAF
                     SQLSDQLDSEIIVNTLDYLFSKFDEICVRNKLEKLKTIGDSHMSVGGIPIRNQTHAID
                     AALAALEMQQFMQKFYKADPQNKIWKLRVGINTGEVVAGVVGKRKFAFDIWGDTVNTA
                     NRLQGCCESNKINISDTTYVLLKDFFDVEYRGEIPIKYKGPTPMYYLLRIKDEFSNDD
                     EGYRPNKKFWDKYLTLVDYSYLKKLDQWIGG"
     gene            3716..4129
                     /locus_tag="PKI65_08040"
     CDS             3716..4129
                     /locus_tag="PKI65_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78421.1"
                     /translation="MNRTIIFISFFLITFNLFNQNIPSYEPLMEEFFLIYKKNTPPEA
                     IDFIFKTNPIIIQKNAEEITQIKLRLSAITAMLGEYHGYEKIREQKYGESLVVVTYML
                     KYSYQPLFYTFTLYYPNNKWQLQTIKFDDKISDLR"
BASE COUNT         1795 a          570 c          649 g         1760 t
ORIGIN      
        1 ttagagaata aaggttgtct aagaattctt tattctcttc cttactcata gcaagatatt
       61 tactggtgtt ttctacaatt tttttcatga attaaaattt ttataaaaat ataaaaatta
      121 tttaacatac aaacttgaaa aacttaaaca tttattaatt ccgattatga ataacctctt
      181 ttcatttttt tgtctttcgc actcatctat tttatcctat taaaaaaatt attcctttta
      241 atttctttgt ttttagatta atttacattt attaatgcat tattatatga tatttgctca
      301 ttttactata atattcaatt ttagttattt tattgcacaa aacaacaatg ttttttctct
      361 ccttttttta ttaattttgt taaaaaaact aacatcttat tttcagtcat ttatatgttt
      421 tatgatattg ggtataagtt gttagctata aaatataagt cagctaatga ataaagacat
      481 aaaatacata ataataagtt tgttattatt atttttaggt gtattgttat atagtcaaag
      541 tattgtaagg tggggattac cgtatgtcaa aaattattat ccttatgaat ataatggtta
      601 ttcacagaac tgggatattt gttttgatag tactggtgtt ttatggttag ctaatggaga
      661 tggattgata agttttgatg gacttaattg gcgtaaatat tttttacctt cttatgatgc
      721 tgtagcatgc atatctatca taaataatag aatatatata ggatcaaaaa atgaatttgg
      781 atatttcaat atatcttcac caagcgaata taaatatgtt tcattaacca ataaagcgat
      841 gaatctatca ttatctaata attatttttt ctattcagcc aaaacggatt atggtgtcta
      901 tatgtctgat ggaaatagcg tggtgagaat acataatgat aatgtagatg tactttttac
      961 taatgtaaaa ggcaacttaa tcagttttaa taatttagca tttttttata attctgctac
     1021 tggtctttat cttttatctg gctctaaatc gttgtatatt aaaggatctt atccacttaa
     1081 tagtaatata tcaatgtttt taccttatac tgatgatact ttattaataa tagacaataa
     1141 aactggcata tataaaggtt gtttaaattt acaaaatatt ataaacccca gtttaactat
     1201 agaagaattt gattttccta acgaaaaaga acttatagct aatgtcattt tagatgctcg
     1261 caaaacctca tttggttata ttttttctac ttatttcggt ggtacttatg tttatgattt
     1321 agattttaaa ttgcaatata ttctgaatca agaatctcaa ttaattagca ttactcacaa
     1381 taaaattgca ttagatccta atgaaaatat ttggttggca atggactatg gaatagctag
     1441 aataaacaca cataatcctt ttactctatt atctaaagta catgggctct catcatctat
     1501 tttagatgtt gaatttttta ataataaatt attagctggg ggttggacta atttatatat
     1561 agaggagctt gataaaagta ctaattattt caaaaaaact gaattagaaa atatttgttg
     1621 gcaattaaaa aaaataaatt ttggtataga tagtttcgtt attgtagctt cttcagatgg
     1681 attatttgca ataaataata aaaataaatt aacaaaaata gatacaggta attttcgtac
     1741 tgtaaatata actcatagtc ctacatatat aaccgcgaca acagataatt tgtttaatgt
     1801 ttatcattat aaaaacaata aatttatcaa agatttatct ctaccattgc cattatacaa
     1861 tactttttcg aatataaata ttaaaatttc tgatagttct tatgttcatt atatatcatg
     1921 tgatgagcct aaaatcataa aaataactat ttcagaaact atacaaatga tgcaagtgat
     1981 aactttgcca atcaagcaaa attctcctac tggttattta ttagaaaaaa tagataatga
     2041 tattttaatt tactcaactg aaaatatatg gcttctaaaa tctaataatg ataaaataac
     2101 attagctgca ccagaattga gaaaatacta tgattctatt cattacatta atattttaag
     2161 agtttttgga gatactatct ggttacaaag atcatctaaa ttgctcccta ataaaccaat
     2221 ttatttacta gtaaaagaaa aaggtagata tgttcataaa aaaacattgc agttatttca
     2281 tgagttagaa atattgtcta ttaaaaatac cccttatgac aatattttat ttgctactga
     2341 tgatggtatt tttgtattaa accaaaaaaa attatttaca aaaacaactc cactaaaact
     2401 ttatgcttca ttagatatat tagcaaaaga tagtacctat ttatttaaaa tattttctgg
     2461 gctagatact gtgcttaaat taccttatcc tctaaataag ctcaaaatag agtggcgaac
     2521 aaacctaata gaaagagaat taactcataa tatcaaattt agttattatc tggaaggcta
     2581 tgaaaatgaa tggtctgaat ggtctactct gcatattaaa gaatataatg atctgaaacc
     2641 tggtaattac acatttcata tcaaagctat agatgaaaat gaacaagcaa aatcaataat
     2701 tagcattcct atacacataa atgtaccttt ttatagaaga gtggtttcct acataattta
     2761 tgcaatagct ggtatattta ttatttatag tatagtctat ttatctaata ggttgataat
     2821 aagacgaaat aagttgttag aagaatatgc tatagctaaa aacaaagaaa atttacaaaa
     2881 aaccttagaa ttagaaaaag aaaaagaaaa agctgataga ttattaataa atattttgcc
     2941 tgtgcgtata gctgaagaat taaaagaaaa aggcaaagta gatgctgaat ttttctcgtc
     3001 agtttctgta atatttacag attttactgc atttagtcag ttaagtgatc aattagattc
     3061 agagattatt gtgaatactt tggattattt atttagtaaa tttgatgaga tttgtgttag
     3121 gaataaatta gaaaaattaa agactatagg cgactcacat atgagcgtgg gcggtattcc
     3181 tatacgtaat caaacacatg ccattgatgc tgctttggcg gctttagaga tgcagcaatt
     3241 tatgcaaaaa ttttataaag ctgatcctca aaataagatt tggaaattaa gagtaggcat
     3301 caatacagga gaagtagtag caggagtagt aggtaaacgc aaatttgctt ttgatatatg
     3361 gggtgataca gtgaatactg caaatcgttt acaaggatgc tgcgaaagta ataaaataaa
     3421 tatatcggat actacatatg tactccttaa agattttttc gatgtagaat atcgtggcga
     3481 aatacctata aagtataaag gacctacacc catgtactat ctacttcgta taaaagatga
     3541 attttcaaat gatgatgaag gatatagacc aaataaaaaa ttctgggata aatatctaac
     3601 cttagtagat tattcttatt taaaaaaatt agaccaatgg attggtgggt aatggaattg
     3661 atttatatat tttgtgttaa ataaaattat taattttata aaaatattta ctattatgaa
     3721 taggacaatt atatttatta gttttttttt aataactttt aacttattta atcaaaatat
     3781 accaagttat gagccattga tggaagagtt ttttttaatt tataaaaaaa atacaccgcc
     3841 tgaggcaata gattttattt ttaaaacaaa tccaattata attcaaaaaa atgctgaaga
     3901 aattactcaa atcaaattac gcctttcagc tattactgct atgttaggag aatatcatgg
     3961 ttatgaaaaa ataagagaac aaaagtacgg tgagagttta gtagtagtta cttatatgtt
     4021 aaaatattct tatcaacctt tattctacac ttttacttta tattatccta ataataaatg
     4081 gcaattacaa acaattaaat ttgatgataa aatctctgat cttagataaa tgctttaaaa
     4141 ttaatttttt ttgtcatatc aaaaaaatct tttatttttg taccttgtac gtaggatgaa
     4201 tttgtcttaa gtattctctt ttgatttcat actaaagggg cattttttaa aaatgcccct
     4261 ttatttatta tttcaaagtc attatacaaa ttttaaaaaa aataagtaat atatatcaaa
     4321 gattataatt tagttatgac ataatatata aagtttacta ggttctataa cgttttatat
     4381 gggttaaaaa ttaaaaaaga gtaatttgtt aatattggtt tatcttttat tggacagtta
     4441 aatattaacc atagagtact ctaataatgc acaaagaaca ctgagaagaa tggaattaaa
     4501 aaggctatgg aactttgtgt aatatcttcg tgctctttgt ggtaaaataa acaacctaaa
     4561 taattaaaaa atagttattt ttttgtaaca atctaaaatt ttattaccca aattataacc
     4621 tgagatatgt tggatgagga gcattggaaa cttgaaaact aatcagtcgg aacggaaaac
     4681 taacatatgt ttcaatcaac atatgttata ttaactaatt ctctataccg ataccacgcc
     4741 catctgtccg aagatgcgag gttgagcatt cgcc
//
LOCUS       DAOPRN010000097         4773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_14451_length_4773_cov_105.692031, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000097 DAOPRN010000000
VERSION     DAOPRN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14451_length_4773_cov_105.692031"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..2480
                     /locus_tag="PKI65_08045"
     CDS             177..2480
                     /locus_tag="PKI65_08045"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOB78422.1"
                     /translation="MKKSILFLLNFLIIIANIYCQTSTIRGFVYNKSTGEPILFTNVY
                     LVGTNYGASTDINGYFTITGIKPGTYTLQITTIGFDTLKEVLNLKPGDIVTKKYYLTE
                     RSISLQKVEVTAIRAEDTLETKTSVIKLTAKQVSSLPSIGMPDIAQYIQVLPGVIFTG
                     DQGGQLYIRGGSPVQNKLILDGITIYNPFHSIGLFSVIETELIRNADIYTGGFNAEYG
                     DRISSIMDINLRDGNAKRFSGMVSISTFGANILLEGPIIRNINNDPNKGNLTFVLSAK
                     NSYLPQSSKVFYKYIDEDGLPFEFQDFYGKITYSTPNGSKMFLNGFNFNDHVKYNDIA
                     DFKWKSNGIGTNFLIIPEGNISLINFKFSYSDYDITLTDPSNLPKESLINGFNLGLNI
                     TNYLQKNSNINYGIELCGFKTDFKFYTEALRLISQTENTTELGIFVKGKIFVNKFIIE
                     PGFRFQYYGSLSEISPEPRLAIKYNVADKFRIKLATGLYSQNLISATDDRDVVSLFYG
                     FLSGSDNLPKTFKGKSVNSRLQKAQHIILGFEINPTDHIAINIEPYFKNFSILQNLNK
                     NKIYDDTEEYSDKPDELKKDMIIECGHAEGIDLSVKYESKSIYIWLAYSFGYIHRDDG
                     YKVYPPHYDRRHNLNFLVTYTWGDLSNNELSMRWNFGSGFAFTQTAGYYPQAIFSNMT
                     DDILQTNDQLGILYGELNNGRLPTYHRLDVSYNHKFYITETVTLNASASITNLYNRDN
                     IFYIDRVTGETVYQLPIMPSVSIRMKF"
     gene            2579..3058
                     /locus_tag="PKI65_08050"
     CDS             2579..3058
                     /locus_tag="PKI65_08050"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF019889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOB78423.1"
                     /translation="MVTLPIFRHNYDIGGAHYFVNLIIDSLPIDFLIDTGATNTVISI
                     DLAKQLESYNKKINLSDNPIITFSDTISEVAIIPIINFQIGEEILNDIIVVATDFSFI
                     KNQSSIKEFKSFDAVLGMDLLTLFKTKIDFKNNKIDFFLPSKKFLKDYYNNLLKNIP"
     gene            complement(3103..4512)
                     /locus_tag="PKI65_08055"
     CDS             complement(3103..4512)
                     /locus_tag="PKI65_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOB78424.1"
                     /translation="MSRKNIFKFILLSSLVFIINNLNGQVISGTIKNAHNLPLYLFYY
                     YGDQKYLIDSTATDTSGNFSFDMPNIGVQYEVRLSKSSQYFYLLRDKDIRLKTVYNPH
                     RIWNYAEDSMKILKSIENKKFYKFLNYQRNWMIGQYWLLQMLRVYPVIDPFHTTLEKE
                     YNKRYSDLAKFMEKIKKDTKYNAHRIALAYYLPNPDWKMPDPWRDSVIVSHYFDYFDP
                     SDPIYLHTFVLPEKMDIYLKLRTNIRDNYGQPIYDEFLFASAAIDFIKHTEKNLAVRN
                     FCISYYLNFFKDQQLEKAFVILYDTYLKNEEGDCGTQEDEWAWARKLADKYKGIELGK
                     PAPDFLIPSNNIFMYELDADYILLVFWATWCSHCMQEIPDIYKATLDFPDSILTTVAI
                     SLDTVPQIVDYLIENFGLNDWIHYCEYRGWNSDIVKAYNVYATPTMFLLDRERKVVAK
                     PISYNELSKVLIKLKRGEL"
BASE COUNT         1701 a          648 c          617 g         1807 t
ORIGIN      
        1 tagtaaatat aacatatgtt aattgaaaca tatattagtt ttccgactga tccgtcatat
       61 ctcaggttat aatttttctt aacgatttaa acaaattaaa attttaatat aaataaatat
      121 tatacatatt taaaatttta tatacatttg tatcatattt tacaatcttt agtagcatga
      181 agaagtcaat tttgttttta ttaaattttt taattattat cgcaaatatc tattgtcaaa
      241 cttcaactat tagaggtttt gtatataata aatccacagg tgaaccaatt ttatttacca
      301 atgtatattt agttggtact aactatggag ctagtactga tattaatggt tattttacaa
      361 ttactggtat taaaccgggc acttatactc tacaaattac aacaataggt tttgacactt
      421 taaaagaagt tttgaattta aagcctggag atattgtaac taagaaatat tatcttactg
      481 aacgttctat tagtttacaa aaagttgaag tgacagctat aagagccgaa gatacattag
      541 aaactaaaac ctctgttatt aaacttacag ctaaacaggt ctcaagtttg ccatccatag
      601 gcatgcctga tatagcacaa tacattcaag tattacctgg tgttattttt actggtgacc
      661 aaggtggaca actttacatc cgaggtggca gccctgttca gaataaatta attttagatg
      721 gtataactat ttataatcct tttcactcta taggtttatt ttctgtcata gagactgaac
      781 tcattcgtaa cgcagatata tatacaggcg gatttaatgc tgaatatggc gataggattt
      841 catctatcat ggatataaat ttaagagatg gcaatgctaa aagattttca ggtatggtta
      901 gtatttctac ttttggagct aacatacttt tagaaggacc aataattcgt aatataaata
      961 atgatcctaa caaagggaat cttacttttg tgttatctgc taaaaattct tatttaccac
     1021 aatcctctaa agttttttat aaatatatcg atgaagacgg actcccattt gagtttcaag
     1081 atttttatgg taaaatcacc tactccactc ctaatggttc taagatgttt ttaaatggtt
     1141 ttaactttaa tgatcatgta aaatacaatg atattgcgga ttttaaatgg aaatctaatg
     1201 gtatagggac aaattttctt ataattcctg aaggtaatat ttcgttgata aattttaaat
     1261 tctcatattc agactatgat attactttaa cagatccttc taatttgcct aaagaatctt
     1321 tgattaatgg atttaatttg ggattaaata ttactaatta tttacaaaaa aattctaata
     1381 ttaattatgg tattgagctt tgtggtttca aaactgattt taaattttat acagaggctc
     1441 tacgtctaat tagtcaaaca gaaaatacta ctgagctagg tatatttgtt aaaggtaaaa
     1501 tttttgttaa taaatttata atagaaccag gatttcgttt tcagtattat ggtagcttat
     1561 ctgaaatatc tcctgaaccg aggctggcta taaaatataa tgtagctgat aaatttagaa
     1621 ttaaattagc tactggttta tattcccaaa atttaattag tgctactgat gatagagatg
     1681 tggttagtct tttctatgga tttttatcag gaagtgataa tttaccaaaa acttttaaag
     1741 gcaaatcagt caattctaga ctacaaaagg ctcaacatat aatattaggt ttcgaaatta
     1801 acccgactga tcatattgct attaatattg aaccttattt taaaaatttc tctatcttac
     1861 aaaatcttaa taaaaataaa atttatgatg atacagaaga atattctgac aaaccagatg
     1921 aattaaaaaa agatatgatc atagaatgtg gacatgccga aggcatagac ttatctgtaa
     1981 agtacgagag caaatctatt tatatttggt tggcatattc ttttggatat atacatcgtg
     2041 atgatggtta taaagtttat ccacctcatt atgataggcg tcataatctt aattttttag
     2101 ttacttatac ttggggcgat cttagcaata atgaattatc aatgagatgg aactttggat
     2161 caggttttgc attcactcaa acagctggtt attacccaca agctatcttt tctaatatga
     2221 ctgatgatat tttacaaact aatgatcaat taggtatttt atatggagaa ttgaataatg
     2281 gtcgtttacc tacgtatcat aggctagatg ttagttataa tcataaattt tatattactg
     2341 aaactgtaac tttaaatgct tctgctagta ttactaatct ttataatcgt gataatatat
     2401 tttatattga tagagtaact ggtgaaactg tttatcaact tcctattatg ccaagtgtta
     2461 gcataagaat gaaattctaa tcttttttga atcataaatc ttttaatcaa atataaattt
     2521 ggtgaaatat gaatcgtaaa ttctatcttg atttaattaa aataataaaa ttattataat
     2581 ggtaacatta cctattttta gacataatta tgatattggc ggagcacatt atttcgtaaa
     2641 tttgattata gatagcttgc caatagattt tttgatagat actggtgcta ctaatacagt
     2701 gatttctatc gatttagcta aacaactcga aagttataat aaaaaaataa atctaagtga
     2761 taatcctatt attactttta gtgatactat cagtgaagtg gctataattc ctattataaa
     2821 ctttcaaata ggtgaagaaa tattaaatga tatcatagta gtagctacag atttttcttt
     2881 tattaaaaat caaagttcta tcaaagaatt taaatctttt gatgcagttt taggtatgga
     2941 cctattgact ctatttaaaa ctaaaattga ttttaaaaac aataaaattg attttttctt
     3001 gccttctaaa aaatttttaa aagattatta taataatctt ttaaaaaaca tcccttaata
     3061 aattgaataa atatttgttt caattaatat aatacatata aattataatt ccccacgttt
     3121 taattttatt aaaacttttg acaattcatt atagctgatt ggcttagcta caaccttgcg
     3181 ctctctatct aataaaaaca ttgtaggagt agcatataca ttataagctt ttactatatc
     3241 actattccat cccctatatt cacaataatg tatccagtca tttaatccaa aattttctat
     3301 taaataatcc acgatttgag gcacagtatc taatgatatt gctacagttg taagtatcga
     3361 atcaggaaag tctaaagtag ctttataaat atctggtatc tcttgcatac aatgagagca
     3421 ccaagtagcc caaaatacta ataaaatata atcagcatct agttcataca taaatatatt
     3481 atttgaaggg atcaaaaaat ctggtgcagg cttacctagt tcaatacctt tatatttatc
     3541 tgctaatttc ctagcccatg cccattcatc ttcctgcgta ccacaatctc cttcttcatt
     3601 ttttaaatat gtatcatata atattacaaa agccttctcc aattgttgat ctttgaaaaa
     3661 atttaaataa taggatatgc aaaaatttct tacagctaga tttttttctg tgtgtttaat
     3721 aaaatcaata gctgcagaag cgaataagaa ctcatcatat attggttgcc cataattatc
     3781 acgtatattt gttcttaatt taaggtatat atccattttt tcaggcaaaa caaaagtatg
     3841 taaatatatt ggatctgatg ggtcaaaata atcaaaataa tgtgagacta ttacgctatc
     3901 tctccaagga tcaggcattt tccaatctgg attaggtaaa taataagcta aagctatacg
     3961 atgtgcatta tatttagtat cctttttgat cttttccata aatttggcta aatcactata
     4021 tcttttatta tactcttttt ctaatgtagt atgaaatgga tctattacag ggtataccct
     4081 tagcatttgc aatagccaat attgccctat catccagtta cgctgataat ttaaaaattt
     4141 ataaaatttt ttattttcaa tcgattttaa tattttcatg ctatcttcag cataattcca
     4201 aatacgatgt ggattataaa cggtttttaa tctaatatct ttgtctctca acaaataaaa
     4261 atattgtgaa cttttgctca gtctcacctc atattgtact cctatatttg gcatatcaaa
     4321 actgaaattc ccactcgtat ctgtagcagt actatctatt aaatactttt gatcgccata
     4381 ataataaaat aaatacagtg gcagattatg agcatttttt atggtccctg atataacttg
     4441 tccattcaaa ttattaataa taaatactaa tgatgataat aaaataaatt taaaaatatt
     4501 ttttctactc atatttttta gctttattaa taaattttac aaatttaaat aaatttctag
     4561 attaataaaa cattacttca ttatttatat ttattttaat aataataaca attttaaact
     4621 atcttattaa atgtttttta gatatttata caataatttt tggttctata acgttttata
     4681 tgggtaaaga ttaaaaaaag agtaatttta taaaaaattt gttaatatta gtttattaat
     4741 ttatagtgta gttaaatatt aaccacaaag ttc
//
LOCUS       DAOPRN010000098         4475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_15706_length_4475_cov_89.681222, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000098 DAOPRN010000000
VERSION     DAOPRN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15706_length_4475_cov_89.681222"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PKI65_08060"
     CDS             <1..509
                     /locus_tag="PKI65_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB78425.1"
                     /translation="NANNNILNIKYTTMAAMAGNYFATASGSTFDIHSWFLTASFNND
                     TLSSYAPLLIRDPYNYSNPDFRLHINSPLLNNSGFVGINENDFSGINVSLYPNPVNDI
                     LNIHFQNNVDKASVQILDIMGRIFYNEVLFDINNDLIINTASLPSGTYIIRVINSNDF
                     LQKSFIKL"
     gene            complement(620..1119)
                     /locus_tag="PKI65_08065"
                     /pseudo
     CDS             complement(620..1119)
                     /locus_tag="PKI65_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557953.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1268..4057
                     /locus_tag="PKI65_08070"
     CDS             1268..4057
                     /locus_tag="PKI65_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOB78426.1"
                     /translation="MNKALNFYLRYIIIALAILFSNLAIGQNLSGYILDNSSKEPIPF
                     VNIRINHSNKGGTTDFDGKFLINNCNVNDTLYISAIGYQSLVVKCSEIPANQTNNFTI
                     YLSKQNEILETIEIKSTKIDNNQIALLNSIKNNTIITTGVSQEIIAKYKESSSTDVVK
                     RLPGITMQDQFIIVRGLSARYNNIYLNDVKAPSMEADQRSFSFDLIPAQFIDYIHIYK
                     SPFSDFSSIFGGALVEIKLREEFDKNSFTFSLGQSINPFTTFKDFQYYGENNTLFLPI
                     KNIFSLPSNFPPSLINSTNENRLQLDKTLNRLWYPTTTIAPTNTNLSLTYSLHKKFKK
                     VDLYSNLMLYSNSNFAKTTFEKAGYNSYNTFLDKPDTNFYFLDTTYESRYMNSALATI
                     NTLFNNNSINLIVLFTDITTDKVTWREGKDYYGGTNIRGNEMLYKKRNILLSELSGKH
                     KLGKNIDIHWFGGITSTTMLQPDNKRLTWVRNDQQDNDSYGQYALNFMFTANPAMSGR
                     LFQELHENEYSAGAKLIQSININDEEGNINFGLFASKLSRNFEARNFGYRISNVMQFD
                     WDITYLPVEQIFTDDYINASNGIILDETTNPSDSYTADETNYSIYIAWQQKLHRFSWY
                     LGLRAEYNTMRLNSYTTDATNPPMDEDKVNYENPILFFSPSFAVGYDINEKMKIKFNF
                     GRTYDKPQFREIAPYAYYDYEAKGVIIGNPDLRNATINHFNLRYEYYFSMYEIFSFDV
                     FYKTFQNAIEYKIIPAGTGLQYSYQNVPNATAIGIETEMHKKILPWMFAIANVSFIKS
                     NVMFKNESIENDRMLQGQSPYVINGGLFFQFDSLDLQASILYNVFGKRITFVGDPYTN
                     NPDIYELPYHNLKINISKTFAKKFMIDFQINNILGSSKEFIQIEKSNTGEDGILIAEK
                     SNIPRIYSFKFTLKI"
BASE COUNT         1642 a          667 c          578 g         1588 t
ORIGIN      
        1 ccaatgcaaa caataacatt ctaaacatca aatacactac tatggctgct atggcaggga
       61 actatttcgc tactgctagt ggctcaactt tcgacattca ctcttggttt ctaactgctt
      121 catttaataa tgatactctt agttcttatg ctcctctgct aataagagat ccttataatt
      181 attctaatcc agattttaga ctacatatta acagtccatt attaaataat tctggttttg
      241 taggaattaa cgaaaatgac ttttcaggta ttaatgtatc tctatatcct aatcctgtaa
      301 atgatatttt aaatattcat tttcagaata acgtcgataa agcatcagta caaatattag
      361 atattatggg gcgaatcttt tacaatgaag tattatttga tattaataat gatttaatta
      421 tcaacacagc tagcttgcca tcaggcactt atattattag agttatcaat agtaatgatt
      481 tcttacaaaa atccttcata aaactataat tctttttatt tttattacaa caaagatggg
      541 gcgaattaca aattcgctcc atctcattat ttttattttg caaattgtga tatttatatt
      601 tctttattaa attaggcgtt taagtaataa tcattttata tggacaaggc tttgcttgaa
      661 ccattttgtt taacagatta tcaaatatat ttatattcat taattgccga ttaaatctat
      721 ttgtatattc ttctatgtaa gcttgcaaag gttctacatg attatttata gcccacaacc
      781 atcctttaaa acccattgtt actctatgca tctctggaaa aatttttcct ttttacctga
      841 atctacttgt atatttgctt ttctatctat agtttctctt ataaaacttc ctaattcttt
      901 tgaattactc ctctctataa ccttccgata tactctacta actcccttat ttcttctttc
      961 ctctacaaaa tttattgtta ccactcctag aacacacata cccattaccc atttgagctg
     1021 tgataaatat tccatgcagg cttcatcatc tggaaatctc tgctgaaatt caaatatcgt
     1081 tatactttca tatttcttat tcagatttaa attttttatt tctgcaaatt tactctttta
     1141 ctcttattat caaatctcct aatttaataa aaatattttg tttaacaatt ttttaatatt
     1201 agttaataat ttaaaaatat taatttaaaa ttaaggttac cttggtttta tacttttgta
     1261 aaaaaatatg aataaagctt taaattttta cctgcgttac attattattg cacttgcaat
     1321 attattttct aatttagcaa taggacaaaa cctgagtggt tatattttag acaattctag
     1381 caaagaacct attccatttg taaatattag aattaatcat agtaataaag gtggaactac
     1441 agatttcgat ggtaaatttt taataaataa ttgtaatgta aatgatacat tatatatatc
     1501 agctattggt tatcaatctt tagttgttaa atgttcagaa atacccgcta atcaaacaaa
     1561 taatttcaca atatatttat ctaagcaaaa cgagatttta gaaacaatag aaataaaaag
     1621 tacaaagatt gataataatc aaatagcttt attaaatagc atcaaaaata atactatcat
     1681 tactacgggt gtgtctcaag aaataatagc taaatacaaa gagtcctcaa gtacagatgt
     1741 ggttaagagg ctaccaggca taacaatgca agatcaattt ataatagtca gaggattaag
     1801 tgctagatat aataatattt atctaaatga tgtaaaagca cctagtatgg aagctgatca
     1861 gaggagtttc tcttttgatc ttattcctgc tcaattcatt gattacatac acatttataa
     1921 atctcctttt tctgatttta gctcaatatt tggtggagct ttagtagaaa taaagttaag
     1981 agaagaattc gataagaatt catttacctt ttctctcggt cagtctatta atccatttac
     2041 tacatttaaa gattttcaat attatggaga aaataatact ttgtttttac ctataaaaaa
     2101 cattttttcc cttcctagca atttccctcc ttcattgatt aatagtacta atgagaatag
     2161 attacaatta gataaaacct taaataggtt gtggtatcct acgactacta tagcaccaac
     2221 aaatacaaat ttatctttaa catattcttt gcataaaaaa tttaaaaaag ttgaccttta
     2281 tagtaattta atgctatata gcaattctaa ttttgcaaaa acaacttttg agaaagctgg
     2341 ttataatagt tacaatacat ttttagataa gccagatact aatttttatt ttctagatac
     2401 aacctatgaa tccagatata tgaatagtgc tttagctact atcaatacct tgtttaacaa
     2461 taatagcatc aacttaattg tattatttac agatataact acagacaaag ttacatggag
     2521 agaaggtaaa gattattatg gaggcacaaa tattcgaggt aatgagatgt tatacaaaaa
     2581 aagaaatatt ttattaagtg aattatctgg gaaacataaa ttaggtaaaa atatagatat
     2641 acattggttt ggagggatca cttctacgac gatgcttcag ccagacaata aaagattgac
     2701 ttgggtcaga aatgatcaac aagataatga tagctatgga caatatgctc ttaattttat
     2761 gtttactgct aatcctgcaa tgtctggaag attatttcaa gaattacatg aaaatgaata
     2821 tagtgctggg gcaaagttaa ttcaatctat aaacattaat gatgaagaag gtaatataaa
     2881 cttcggatta tttgctagca aactttctag aaatttcgaa gcaagaaatt tcggttatag
     2941 aatatctaat gtgatgcagt ttgattggga tataacatat ttgcctgtag aacagatatt
     3001 tactgatgat tacatcaatg cgtcaaatgg tattatacta gatgaaacta caaatcctag
     3061 cgattcatat actgctgatg aaacaaacta ttctatttat attgcatggc agcaaaagct
     3121 acatagattt tcatggtatt tgggtttgag agcagagtat aacactatga gactaaattc
     3181 atatactaca gatgctacta atcctccaat ggatgaagat aaagtaaatt acgaaaatcc
     3241 tatactgttt tttagtcctt catttgcagt aggctatgat attaacgaaa aaatgaaaat
     3301 caaattcaat tttggaagaa catatgataa accacaattt agagaaatag caccatatgc
     3361 atattatgat tacgaagcca aaggagtgat aataggcaat cctgacttga gaaatgctac
     3421 aataaatcat ttcaacctac gatatgaata ttatttctct atgtatgaaa ttttttcatt
     3481 tgatgttttt tacaaaacct ttcaaaatgc tattgaatat aaaattatac cagcaggcac
     3541 tggtttacaa tattcatatc aaaatgttcc taatgctact gcaataggaa tagaaacaga
     3601 aatgcataaa aaaatattac catggatgtt tgctattgct aatgtttctt ttataaaaag
     3661 taatgtaatg tttaaaaatg aaagtataga aaacgataga atgctacaag gccaatctcc
     3721 ctatgtaata aatggtggat tattttttca atttgattct ttagacctac aagcgagtat
     3781 attatataat gtttttggca aacgtataac ttttgtagga gatccatata ctaataaccc
     3841 agacatttat gaactgccct atcataatct aaaaattaat atttccaaaa catttgctaa
     3901 aaaatttatg attgattttc aaattaataa tattttagga agtagtaaag aattcataca
     3961 aatagaaaaa tcaaatactg gcgaagatgg tatattgatt gcagaaaaaa gcaatattcc
     4021 cagaatatat agctttaaat tcaccttaaa aatataatct cctaaaaaaa atattggcat
     4081 aaaattttta tattcattcc ataatctgat tacttttgtg ctatctgaaa aaagaaaacc
     4141 gagagttgat tttagatagc gaaagactaa acaatatcat attattaaaa aaggtattaa
     4201 aaaaagatct attaaataga attaatctaa tataaagtta agatatgtaa aggagagttt
     4261 gttttaaatt tttttcaaaa acatttaaat gttatagatt aaatatgttg atgacatcag
     4321 atcactatac cgctcccatg cccgcctatc cgacgccgcc aaattcaaca acctcccagt
     4381 gtcttctata atcttatccg ccaaatactt tccccttaca acaaaccaca ttatgtagtt
     4441 tgttaaatac ttcgttgcaa ctccacgaaa cttat
//
LOCUS       DAOPRN010000099         4212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_16961_length_4212_cov_108.930479, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000099 DAOPRN010000000
VERSION     DAOPRN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16961_length_4212_cov_108.930479"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..1603)
                     /gene="hydF"
                     /locus_tag="PKI65_08075"
     CDS             complement(386..1603)
                     /gene="hydF"
                     /locus_tag="PKI65_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOB78427.1"
                     /translation="MTTKRGNELRPHIGIYGRRNYGKSSLINKITKQDIAIVSDIAGT
                     TTDIVKKAIEISQIGPVVIIDTAGIDDMGELGKLRVKKTLNSLDMIDFAILLITHNTF
                     DKPEKNIIDEFKKRNIPFIIIHNKSDLEPLSLRLKDKIKRDYGVPIIDFSIKNDNVQD
                     LIELIKNNLPETAFKTTNILSDIISPGDIVLLITPIDAEAPEGRLILPQVQTIRQILD
                     NNAIAIVCKENEAESIIKEKFPNPSLVITDSQVFSKVDKIVPRHILLTSFSILLARQK
                     GNFEYYLKGTPYIDKLNDNDKILILESCTHDVNCDYDIGRVKIPKWLCDYTGKNLEFK
                     VVSGLEKIDNIKDFAMVIQCGGCVVTKKQIELRLRPAIEAGIPVSNYGMTIAYVKGIF
                     NRAIQPFIDLQSS"
     assembly_gap    1634..1733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2112..3500
                     /locus_tag="PKI65_08080"
     CDS             2112..3500
                     /locus_tag="PKI65_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOB78428.1"
                     /translation="MFTKSIYINRRKNLKSKGLNGIGLFLGNTESPMNYPANTYRFRQ
                     DSSFLYFFGLNLSNLAAVIDFDNGEEYLFGDDVDIDDIIWMGPQPKMNELGNQIGIKN
                     VLPINKLYDFISKQIQLKRTIHYLPPYRAENKILLNELLDIPIKSLKNLSSTELVRAV
                     VSLRSIKAEEEIQEMEIAAEIGYKMHMCVFKMARPGIKEQEIAGMMDGIAYQYGQGPS
                     FPTILSIHGETLHNPYHNNIMQDGDLLLADAGAQANSYYASDFTRVTPVSGKFTQKQK
                     EIYEIVRRANQNTIEISAPGKKYIDIHLAAAKVIANGLIELGLMKGNVDDAVAQGAHT
                     LFFVHGLGHMIGLDVHDMEDLGENYVGYDDELRRSEEFGTGSLRFAKTLKPGMIVSNE
                     PGIYFIPALIEKWKAENKFSEFINYSQVEKYMNFGGIRLEDEILITEDACRLIGKRLP
                     IEIKDVEDAMKD"
     gene            3573..4178
                     /locus_tag="PKI65_08085"
     CDS             3573..4178
                     /locus_tag="PKI65_08085"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOB78429.1"
                     /translation="MFSGIIEQVGRVTKVEREGTNIHFTIKAPMASDLKIDQSVCHDG
                     VCLTVVWVDKKNLSYRVTAIDETLNRTCLKNWKDAYEVNLERSVPVNGRFDGHIVQGH
                     VDQIGIVTKIEVLDGSWKYFIEYNPTYGNITVEKGSIAVNGVSLTVVDSIKNLFSVAI
                     IPYTYENTNFHNFDIGTIVNLEFDIIGKYIKKILEEYEIIK"
BASE COUNT         1487 a          522 c          642 g         1461 t
ORIGIN      
        1 aggtcgagga ataacaaaga aaagttatgg gctttagccc tgaaatagtc agtaccgcag
       61 attatcagat gaataaccaa aaaaatatca atgacaaggt ataacacaaa atattaacat
      121 ttaacgaaga atatgaagaa ttcataaaag attttcaggg ctaaagcccg gttaattgtt
      181 ttattgagac cacgacctaa aggtcgtggt taatagatgt ttgaattaag aaaacctttt
      241 aagtcgaata ataaaacaaa gagtgattaa ttaacatcga cgttttaggt cgaatagcaa
      301 tgagctgctt attaacctat attttatgta taatatattt gtttataaac ttttaacttt
      361 acaaacttta taaactttat gaactttatg aactttgtaa atctataaat ggttgaattg
      421 ctctattgaa gatgcctttt acataagcaa tagtcatacc atagttggaa actggaatac
      481 cagcttctat ggcaggtctt agcctgagtt caatttgttt tttagtaacc acacaaccac
      541 cacattgtat taccatagca aaatccttta tattatctat tttctctaag ccactcacaa
      601 ctttaaattc taaattttta cctgtgtaat cacaaagcca ttttggtatt ttcactcttc
      661 ctatatcata gtcacagttc acatcatgcg tacaactttc taatattaat attttatcat
      721 tatcatttag tttatctatg tatggtgtac cttttagata atattcaaaa ttaccctttt
      781 gacgtgccaa taatatgcta aatgatgtaa gaagtatgtg tcgaggtact attttatcaa
      841 ctttcgaaaa aacttgacta tcagtgataa ctaatgatgg attaggaaat ttttctttga
      901 taatgctttc agcttcattt tctttacata ctattgctat tgcattatta tccaaaattt
      961 gcctaatggt ctgcacttgt ggcaaaatca atctaccttc gggagcttca gcatcaatag
     1021 gtgttattaa taaaactata tcacctggtg atattatatc tgacaatata tttgtagtct
     1081 taaatgcagt ctctggtaaa ttgtttttta tcaattctat taaatcttga acattatcat
     1141 ttttaataga aaaatcaatt ataggaactc cataatctcg ttttatttta tcttttaatc
     1201 gtaaagataa tggttccaag tcagatttat tatgaataat tataaaagga atatttcttt
     1261 ttttgaattc atcaataata tttttttctg gtttatcaaa agtattatgt gtaattaata
     1321 aaatggcgaa atctatcata tctaaagaat tcaaagtttt ttttacacga agcttaccta
     1381 attctcccat atcatctatt ccagcagtat caataatgac tacaggaccg atttgagata
     1441 tttctattgc tttttttact atatcagtag tagtacctgc tatatcacta acaatagcta
     1501 tatcttgttt ggttatttta tttattaatg agcttttacc gtaattacgt cggccatata
     1561 tacctatatg tggtctaagt tcattacctc ttttagtagt cataattata ctttttttta
     1621 atattttgta gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngttcatt
     1741 acctctttta gtagtcataa ttatactttt ttttaatatt ttgtagttgt tttttacaaa
     1801 tttactcaaa atagaataaa aaatttcatt ctataacgtt ttatatggtt aaagattaaa
     1861 aaaaagtaat tgtacaaaaa taaatttgtt aatattgctt tatttattat tggacagtta
     1921 attattaacc acaaagttct caaagaatgc acaaagaacg caaagaagta taaaattaaa
     1981 agggatacaa atggtacttt gtggactttg tgtaataact tggtgtcctt tgtggtaaaa
     2041 aaataaccta aataatttat ccacaataaa cgttatagaa taaaaaattt cattattttt
     2101 accaaaaaat tatgtttaca aaatcaattt atattaatcg tagaaaaaat ttaaaatcaa
     2161 aagggttaaa tggtataggc ttatttcttg gtaatacaga atcaccgatg aattatccag
     2221 caaatactta caggtttaga caagatagta gttttttata tttttttgga ttaaatttat
     2281 ctaatttggc agctgtaata gactttgata atggagaaga atatttattt ggcgatgatg
     2341 tagatataga tgatataata tggatgggac cacaacccaa aatgaatgaa ctaggaaacc
     2401 aaataggtat taaaaatgta ttacctataa ataaattata tgattttata tctaaacaaa
     2461 tacagcttaa aagaacaata cattatttac ctccatatag ggcagagaac aaaattttat
     2521 taaatgaatt attagatata cctataaaaa gtttaaaaaa tttatcgtct accgaactag
     2581 tgagagctgt tgtatctcta cgttctataa aagccgaaga agaaatacaa gaaatggaaa
     2641 tagctgctga gattggttac aaaatgcata tgtgtgtttt taagatggca cggccaggaa
     2701 taaaagaaca agaaatagct ggtatgatgg atggtattgc ttaccaatat ggtcaagggc
     2761 catcttttcc tactatcctt tctatccatg gcgaaacttt gcataatcca tatcataata
     2821 atataatgca agatggtgat cttttgcttg ctgatgcagg tgctcaagct aatagttatt
     2881 atgctagtga cttcactcgt gtaactccag taagcggtaa atttacacaa aaacaaaaag
     2941 aaatatacga aattgttcgt agagcaaatc aaaatactat agaaatatct gctcctggta
     3001 aaaaatatat tgatattcat ttggctgcag ccaaagtgat agcaaacggt ttaatagaat
     3061 taggtttaat gaaaggtaat gtagatgatg ctgttgctca aggtgctcat acactatttt
     3121 ttgttcacgg attaggtcat atgataggtt tagatgttca cgatatggaa gatcttggtg
     3181 aaaattatgt aggttatgac gatgaattgc gaagatctga agaatttggt acaggttctc
     3241 ttcgttttgc taaaactttg aaaccaggca tgatagtaag taatgaacca ggtatttatt
     3301 ttattcctgc attaatagaa aaatggaaag ccgaaaataa attttcagaa ttcataaatt
     3361 acagtcaagt agaaaaatat atgaatttcg gtggtataag gctagaagat gaaattttaa
     3421 taactgagga tgcttgtagg cttattggaa aaagattacc aattgaaatt aaagatgtag
     3481 aagatgctat gaaagattaa tagatgattt gtataaatta atttattatt atttttgttg
     3541 attattttaa tgattaaaaa taaattaaat ttatgtttag tggtattata gaacaagtag
     3601 gaagagtaac aaaagtagag agagaaggta caaatattca ttttactatt aaagcaccta
     3661 tggcttctga tctaaaaata gatcaaagtg tttgccacga tggtgtttgc ctaacagtag
     3721 tatgggtaga taaaaaaaat ttatcatacc gtgttacagc tattgatgaa actttgaata
     3781 gaacctgttt aaaaaattgg aaagatgctt acgaagttaa tttagaaaga tccgtacctg
     3841 ttaatggtcg cttcgatggg catatagttc aagggcatgt agatcaaata ggaatagtta
     3901 ctaaaataga agtgcttgat ggtagttgga aatattttat agaatataat cctacgtatg
     3961 gaaatataac agtagaaaaa ggctctattg ctgttaatgg agttagtctg actgtcgttg
     4021 atagtataaa aaatttattt tcagtagcta ttattccgta tacttatgaa aatactaatt
     4081 ttcataactt tgatatcggt actattgtta atttagaatt tgatataatt ggtaaatata
     4141 ttaaaaaaat tctagaagaa tatgaaatca taaaatgatg attttatgat tgatccaatt
     4201 tgtatagtat at
//
LOCUS       DAOPRN010000100         4113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_17490_length_4113_cov_201.430015, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000100 DAOPRN010000000
VERSION     DAOPRN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17490_length_4113_cov_201.430015"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKI65_08090"
     CDS             <1..614
                     /locus_tag="PKI65_08090"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78430.1"
                     /translation="HKILAALQSFEDNINFFGVVEMDELLMDYSEKGRIYKNAEELKR
                     ARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMPKDGVMCSSNKKAF
                     KKVRRNVIKHKEIVTSHRQKRGIYSLSTVKKKASEFTNWIYFKFRGVATKYLQNYIMW
                     YVVMGKYLTGVTVSNTGRILNLASSDRWAWYRYRELVNITYVN"
     gene            882..2339
                     /locus_tag="PKI65_08095"
     CDS             882..2339
                     /locus_tag="PKI65_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78431.1"
                     /translation="MKKIYVQIIFLLILFTSTFFILSSRWSEQVDYRSIAHIFSLITF
                     ERWDNEGAQHFYYAPVVNYNNPIDMFVDIYPRVFDNKGNNYYLSHPPGSFLLSYSIIK
                     SFHLPIDQVSLQKILFIYFVFSIIGMAILLNILFSKKIIPILVGIFVYTFHPLILYSF
                     TFYHFAETIGLVFFIWILVFHLLVRKNPNNKLYVFLHNIFLFIFLLIDWVAFIYAIFY
                     IIYSFKKKNRKDALLIIILTIIAYAIIFIQYSSIDGVQPFMHHIWLRYIDRIGIFSSI
                     FKNNGFGVLLSEILRLIFNCIHNLLKGTGILLFLVIIPMIKDKSIFKENHNLNFIFYW
                     VFVPIFLFSILVFSVALTHYIYTAKFVLFICLIIAYVFDKYFINIEQKWFQASVLLIF
                     IITITWSLNIYNQFYEYDPVQLKLDNEAKIFSQKANQSDRCIYILGKNQNPHELVYIT
                     YKAKRNLMLFYNFDDFNNWYLNNLNIDCYYIISNN"
     gene            complement(2814..3806)
                     /gene="obgE"
                     /locus_tag="PKI65_08100"
                     /pseudo
     CDS             complement(2814..3806)
                     /gene="obgE"
                     /locus_tag="PKI65_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800096.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
BASE COUNT         1447 a          514 c          563 g         1589 t
ORIGIN      
        1 gacacaagat attggcagct ctacaaagtt tcgaagataa tatcaatttt ttcggggtag
       61 tagagatgga tgaactattg atggattatt ccgaaaaagg cagaatatac aaaaatgccg
      121 aagagctgaa gcgagctagg aagcaaaagc caaagaaagt agccgtattg gcagctactg
      181 acagagctgg caacttatta ttcaaaaaat ttgatggcaa aaagctaagt tcagaacaga
      241 tagaacaatt tctgaaggct aaaatgccaa aggatggagt catgtgtagc agtaataaaa
      301 aagcattcaa aaaggttaga aggaatgtaa tcaagcacaa agaaatagta accagccata
      361 ggcaaaagcg aggtatatat agcctaagta ctgtcaagaa aaaggctagt gaattcacga
      421 attggatata tttcaaattt aggggtgtag ctaccaaata cctgcaaaac tacattatgt
      481 ggtatgtagt gatgggtaaa taccttacag gcgtgactgt aagcaatact gggcggatac
      541 tcaacctcgc atcctcggac agatgggcgt ggtatcggta tagggagtta gtaaatataa
      601 catatgttaa ttgaaacata tgatagtttc ccgattcatt cttcacttac ctaataatat
      661 atgtttttgt taaaatttta gtttgtaaat gatttctaca ttattaattg ttactattat
      721 aagttggaag ttttgcataa ttcataatta tattattaaa taattactta tgtttttgta
      781 tgatttaaat ttaatatttt ataaaaatag acatattact aagtacctat ttttttctta
      841 aatttgacat tgattattaa ttatttaaaa atcagttatg gatgaaaaaa atttatgtcc
      901 agattatatt tttattgata ttatttacta gtacattttt tattttatca tctcgatgga
      961 gcgaacaagt tgattaccgg agtatagcac atattttttc attaatcact tttgaaagat
     1021 gggataatga aggcgcacaa catttttatt atgcgcctgt tgttaactac aacaacccta
     1081 tcgatatgtt tgtagatatt tatcctagag tttttgataa taaaggcaat aattactatt
     1141 tgtcgcatcc accaggtagt tttttgctat cttacagtat tattaaatca ttccatttac
     1201 ctattgatca agtaagttta cagaaaatac tttttatata ctttgttttt tctatcattg
     1261 ggatggctat tttacttaat atactttttt ctaaaaaaat aattcctata ctagttggta
     1321 tttttgttta tacctttcat ccattgatat tatatagctt tactttttat cattttgctg
     1381 aaactatagg acttgttttt tttatatgga ttttagtttt tcatttatta gtacgaaaaa
     1441 atcctaataa taaattatat gttttcttac ataatatatt tctttttatt tttttactaa
     1501 tagattgggt agcttttatt tacgcaatat tttatattat ttatagtttt aaaaaaaaga
     1561 atagaaaaga tgctttatta ataatcatat taactatcat agcatatgca attattttta
     1621 tacaatatag tagcattgac ggagttcagc ctttcatgca tcacatttgg ttaagatata
     1681 tagatagaat tggaattttt agcagtattt ttaaaaacaa tggttttggg gttttgttat
     1741 cagagatatt gagattaatt tttaactgca ttcataattt attaaaaggt acaggtattt
     1801 tactattttt ggttattata ccaatgataa aagacaaatc aatttttaaa gaaaatcata
     1861 atttaaattt cattttttat tgggtatttg tgccaatttt tttgttttca atattagttt
     1921 tttcagtagc tcttacacat tatatttata ctgcaaaatt tgttttattc atttgtttga
     1981 ttatagctta tgtttttgat aaatacttta taaatattga gcagaaatgg tttcaagctt
     2041 ctgttttatt gatttttatt attactatta catggagtct aaatatatat aaccaatttt
     2101 atgaatatga tcctgtacaa ttaaaattag acaatgaagc aaaaatattt tcacagaaag
     2161 ccaatcaatc ggatagatgt atttatattt taggtaaaaa tcaaaatcct catgaattag
     2221 tctacattac ttataaagca aaaagaaact taatgctatt ttataatttc gatgatttta
     2281 ataattggta tttaaataac ttgaatattg attgctatta tattatatct aataattaaa
     2341 taattattat aattatattt ttttctcaaa aaatatatat ttttatgcaa gttttcattt
     2401 attttaattt tgaaatgttg caaatataaa tattatatca gtaatagtgc aagaagtaga
     2461 tgggtagaga atgaaaaaag gtaaaaaaat aaaaatggat gaaatgcaga tagtgaggaa
     2521 agacaaaaag tcgaatatat tatttgttgt taattaaaaa atatgttatt tgtaacttca
     2581 aatttgcttt tcacttttat taagacaaga gtttaaattt aatggaatgt ttactaaacc
     2641 tacttgtagt gtacatagtc agattctatt tagaaatttt attgagtttg gtgaaatatt
     2701 cagaatttat aattttttgt ttttctgatt attaaattta atgtaaaaaa cgtattctat
     2761 tatttgaagt atttttattc atgatagtcc atttacatgt tttcctcagg gtgctcatca
     2821 tttaatacaa accataaatg atcttttagc tctttaatgc cataaccagt aattgaagat
     2881 ataaatatcg gatttatttt gcttaatttt tcttttaaat aatttatttc tgaactatct
     2941 gcgaggtctg tcttagtaat agctacgaca cgtttcttag aaagtaaagt tggattatat
     3001 ttttctaatt catttaataa aatatgatat tcagataaat gatctttaga tgtaataggg
     3061 atcataaaaa gtaaaatgct atttctctca atatgtctaa gaaatctcaa tcccaatcct
     3121 ttgccttcac tagcaccttc tattatacct ggtatatcag ctatagtaaa ggattgatag
     3181 tctctataag gaactacacc gagttgaggt aataaagtag tgaaaggata atcagcaatt
     3241 ttaggtttag cagcagacaa tacagagagt aatgtgctct tacctgcatt agggaaaccg
     3301 actaatccaa catctgctaa taattttaat tcaaaaataa aatttccgct tttataatct
     3361 tcacctggtt gagcaaattt gggtgcttgc cgagtagagc ttttaaaaaa ggagtttcct
     3421 ttaccacctt tcccaccttt taataagata aattcttgtt tatcatcatt tatttctgta
     3481 attacctgtc cgctttcagc ttcttttatt actgtaccaa ttggtacttc tataaaagca
     3541 tctttcccat cagcaccagt ttttaatttt ccactgccag attgaccatt ttcggctttt
     3601 attattttag tatattttaa atgcaacaag gtccataagt ttttattacc ttttacaata
     3661 atgtgtccac ctcttccacc atcacctcca tcaggtcctc cacgaggtat atatttttct
     3721 ctacgaaaat gtacagatcc acttccgcct ttaccagatt gacaatatat tttaatataa
     3781 tctatgaaat tcccttcttc agacattgtc aaaatactta attattaagt tataaaatta
     3841 aacttatttg tatttaataa aatatttcta caaaattaat aaaaagttat aacattttag
     3901 taaattaacc aaggatttaa cattaaattt tttaacaaaa acattattac atcaactaaa
     3961 atattagtta atataaatgg atatgattat attgaaaaaa taataaaaaa tttatattta
     4021 caatttggtt ttaataataa tgaatagatg aaatgaataa accagtaaat gtttaagttt
     4081 ttcaagttta tatgttaaat aatttttata ttt
//
LOCUS       DAOPRN010000101         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_17670_length_4076_cov_106.490923, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000101 DAOPRN010000000
VERSION     DAOPRN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17670_length_4076_cov_106.490923"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..407
                     /locus_tag="PKI65_08105"
     tRNA            335..407
                     /locus_tag="PKI65_08105"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:368..370,aa:Pro,seq:cgg)
     gene            411..483
                     /locus_tag="PKI65_08110"
     tRNA            411..483
                     /locus_tag="PKI65_08110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Thr,seq:cgt)
     gene            544..1896
                     /locus_tag="PKI65_08115"
     CDS             544..1896
                     /locus_tag="PKI65_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOB78432.1"
                     /translation="MSNRENFSSTFGIIAAAAGSAVGLGNIWRFPYVAGENGGGAFLL
                     IYIFIVLILGIPLLISEMMIGRRAQANVIGSFKKLGPKSSIIIGIIGLLAAFIILSFY
                     STVGGWTIHYIYASISGEVFKLDYQQYFTNFKDKSFLPLLWQVIFLIMTAIIILGGVK
                     KGIENCSKVLMPLLILMLIILMIRAVTLPGSKSGIDFFIKPDFSKVTGKTFLAAMGQA
                     FFSLSIGMGAMLTYGSYIKKDQNLTFIGFSITIADTLIAILAGFVIFPTLFSFNVAPN
                     QGPGLIFVTLPAIFDQMPWGAGFSTLFFILLLIASLTSSISLLEVIVAVLKEELKWSR
                     TLSTIITTILVMCLGVFTTLSWNTFQNLFITTAQGNCMYIFDICDYFSSNILLPLGGF
                     LIMLYVGWYLKKDVFYEELSAGVNNKFTLSLRSMIFFIVKYIAPLVIILVFLNGLGII
                     "
     gene            complement(2145..3752)
                     /locus_tag="PKI65_08120"
     CDS             complement(2145..3752)
                     /locus_tag="PKI65_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOB78433.1"
                     /translation="MKKIIVFIIIFYFSTFIYSQKNTVNIEPVNDIISTQVQKFSQLL
                     NLLDRYYVDTVNFSHLTEEAIKGLLSELDPHSVYIPAKDVKSVEEPLVGSFEGIGITF
                     QIIRDTINVIEVISGGPAEIVGIFPGDKIIKIDDSVAYGSYVTNDYVTKKLRGPKGTS
                     VKVTIKRVNHSELIDFIIIRDKIPLNSINAAYMLEPGIAYIKVDRFARNTINEFKNTI
                     NELKKEKIDFLILDLRGNGGGYLSTAVQLADEFLDAGKLIVYTQGRSMPVNKYASSAK
                     GEYQDGKLIVLIDERSASASEIVAGAMQDWDRAILMGRRSFGKGLVQNPYYLLDGSLV
                     RLTIAKYYTPTGRCIQKPYSNSNEYYHDIENRLKKGELMHPDSVHLPDSLKYYTLNKN
                     RLVYGGGGIMPDVFIPLDTSLSSELSNQLVRKNIYNMYVIDIISKEREKLLNSYPTKE
                     VFRKKFIIYDDLFNNFLNYAKNNEIEINNDELERSKSIINCVLKASIARYLYGVSGYY
                     YNIYEIDYELKKAVELGKDGKVFEQLY"
     gene            3887..>4076
                     /locus_tag="PKI65_08125"
     CDS             3887..>4076
                     /locus_tag="PKI65_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOB78434.1"
                     /translation="MNSKEIFSLALNLEKPWYIKDIIFEKPNSKKFGRLTIQIDFERG
                     AKFTIEGGEECSVYDTKIM"
BASE COUNT         1366 a          589 c          565 g         1556 t
ORIGIN      
        1 tagtgaattt aacatatgtt aattgaaaca tatattagat tccccaaatt tcccgaacaa
       61 cttcatttta cccctatttt ttatattctt tattattaat tactttaaca tacatattct
      121 attataaagc ttatcattac agtatattat agatttttga ttattacaca atcaattcta
      181 ttactattta tatatttttt gttaaaaacg taaaaattta ctaactaatc aattacattg
      241 atatcattca ttattttata aaaatctaaa tttttatttc atacctaact ttttatgtat
      301 ttacataaaa tacattaatt ttgcaaaaac ataacgggat gtagcgcagt tggtagcgca
      361 cttcgttcgg gacgaagtgg tcgctggttc gaatccagtc atcccgattt gccatcctag
      421 ctcagtaggt agagcagctc actcgtaatg agcaggtcgt gagttcgagt ctcacggatg
      481 gctcttaatt ttattaaatt tttttctatt ttttaaattt ttataagttt gtaaaaattt
      541 tttatgtcta atagagaaaa tttttcttca acttttggta ttatagcggc tgcagcgggt
      601 tctgctgttg gtttaggaaa tatatggaga tttccttatg tagcaggtga aaatggtggt
      661 ggtgcttttt tattaattta catatttatt gtactaatct tgggaattcc attattaatc
      721 tctgaaatga tgatagggcg cagagcacaa gcaaatgtca taggaagttt caaaaaatta
      781 gggcctaaaa gtagtatcat tataggtatc ataggattac tagcagcatt tattattttg
      841 tcattttata gtacagttgg tggctggact atacattaca tatatgcaag tattagcggc
      901 gaagttttta aattagatta tcagcaatat tttacaaatt tcaaggataa aagtttttta
      961 ccattattat ggcaagtcat atttttaata atgacagcga taataatttt aggaggagta
     1021 aaaaaaggaa tagaaaattg ttctaaagtg ctaatgccat tattaatatt gatgttaata
     1081 atcttaatga ttagagcagt tacattacca ggaagtaaga gtggaattga tttttttata
     1141 aaacctgatt ttagtaaagt aacgggtaaa acgtttttag cggccatggg gcaagcattc
     1201 ttttcactta gtatcggtat gggggcaatg cttacttatg gttcttacat aaaaaaagac
     1261 caaaatctaa cttttatcgg tttttctatt acaattgctg atactttaat agctatcctt
     1321 gctggttttg ttatttttcc tactttattt tcttttaatg tagcacctaa tcaaggccca
     1381 ggattgatat ttgttacttt accagctatt tttgatcaga tgccttgggg ggcaggtttt
     1441 tctaccctgt tttttatcct tttactcata gcttccctta catcttctat atctttatta
     1501 gaggtaatag tcgcagtact aaaggaagaa cttaaatggt ctagaacatt atcaaccatt
     1561 attactacta tattagttat gtgtttaggg gtttttacta ctttgtcttg gaatacattt
     1621 caaaatcttt tcatcactac tgcacaaggt aattgcatgt acatttttga tatttgtgat
     1681 tatttttcat caaatatctt acttccttta ggtggttttt taatcatgct ttatgtgggt
     1741 tggtatttaa agaaagatgt tttttacgaa gaattatctg ctggtgttaa taacaaattt
     1801 actctatcat tgagatcgat gatttttttt attgttaaat acatcgcacc cctagtgata
     1861 attttagtat ttttaaatgg attaggaatt atttaatttt aaaatcaact gttaacatac
     1921 aaagaaaaat ataaatcata ttagaattat taaaatatgt taatttaatt aaaaaaaatt
     1981 tgtattgtaa aaacaaaagc attagataag actagaaaaa aaagaaaaga aaaactaaaa
     2041 gacagagatg agaagcttag aaatttgata tttttcgctc ttgtagcaaa tccattttaa
     2101 taaaaccagt cattaattaa taaatgatat aaataatatt tcgattaata caattgttca
     2161 aaaaccttac catctttccc aagctctaca gcttttttaa gctcataatc aatctcataa
     2221 atgttgtaat aataaccact aactccatat aaataacgtg caatagaggc tttcaacaca
     2281 caatttataa tcgattttga tctttctaat tcatcattat taatttctat ttcattattt
     2341 tttgcataat ttaaaaaatt attaaataaa tcatcatata ttataaattt tttacgaaaa
     2401 acttcttttg ttgggtaact attcaacaat ttttctctct ccttacttat aatatctatt
     2461 acatacatat tataaatatt ttttctaact aattgatttg ataattcaga acttaagctt
     2521 gtatctagag ggataaagac atcaggcatt ataccacctc caccatatac aaggcgattt
     2581 ttatttagag tataatattt caatgaatct ggcaaatgta cagaatcagg atgcataagt
     2641 tcaccttttt ttaatcgatt ttctatatca tgataatatt cattactatt agaataaggt
     2701 ttttgtatac atctacctgt aggcgtataa tatttagcta tagtcaatct aacaagtgaa
     2761 ccatctaata aataataagg attttgtacc aatcctttgc caaaagatct tcgtcccatc
     2821 agtattgctc tatcccaatc ttgcatagca ccagcgacta tttcgctggc cgaagcacta
     2881 cgttcgtcta tcaatactat taatttgccg tcttggtatt cacctttagc agaagatgca
     2941 tacttgttta caggcatact cctgccttgg gtatacacta ttaatttacc tgcatctaaa
     3001 aattcatctg ctaactgcac tgcagtagat aagtatcccc caccatttcc acgtaaatct
     3061 agaatcaaaa aatctatttt ttcttttttt aattcattaa tagtattttt gaattcatta
     3121 atagtatttc gagcaaatct gtctaccttt atataagcaa tgccaggctc tagcatatat
     3181 gctgcattga tggagttgag tggtatttta tctctaataa ttatgaaatc tattagctct
     3241 gaatggttta ctctcttgat tgtaactttt acagatgtac cttttggacc tctaagtttt
     3301 ttagttacat aatcattagt aacataactt ccataagcaa ctgaatcatc tatttttatg
     3361 attttatctc caggaaatat gcctactatc tctgctggtc cgccgctaat cacttctatt
     3421 acatttatcg tatcacgtat tatctgaaat gttattccta taccttcaaa gctacctacc
     3481 aatggctctt ctacactttt tacatcttta gctggtatat atacactatg aggatctaat
     3541 tctgataata atcctttgat agcttcttct gttaaatgag aaaaatttac tgtatctaca
     3601 taatatctat ctaatagatt tagtaattgc gaaaattttt gtacttgagt agatataatg
     3661 tcattgacag gttctatatt tacagtgttt ttctgactat aaataaaagt agaaaaataa
     3721 aaaataataa taaacacaat tatttttttc atttatgttc tttttttttt attaatttac
     3781 aaagttaaag ttaaatttta ttatactact aaataaaaaa aaactttggt tctataacgt
     3841 tttgagtggg taaataaaaa atagtaaatt tggataaaaa aaagatatga attcaaaaga
     3901 gatatttagt ttggcattaa atttagaaaa gccatggtat atcaaagata ttatttttga
     3961 aaaacctaat tcaaaaaagt ttggtagatt aacgatacaa atagatttcg aaagaggagc
     4021 taaatttact atagaaggag gggaggaatg ttcagtgtat gatacgaaaa taatga
//
LOCUS       DAOPRN010000102         3962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_18263_length_3962_cov_78.222165, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000102 DAOPRN010000000
VERSION     DAOPRN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18263_length_3962_cov_78.222165"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PKI65_08130"
     CDS             complement(<1..447)
                     /locus_tag="PKI65_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOB78435.1"
                     /translation="MAARIKRLRKIIELPVVKGFKPYGVTNTKQNEKPIILLYEEYEA
                     IRLCDYKMYTHLQASEIMNISRPTFTRIYASARQKIAKAFAEGREIKINGGNVYYDSP
                     WYHCNTCSCYFNNPNIDDVIRNCPLCGSQDIVSDNFSPVSQEKLVDD"
     gene            complement(764..1411)
                     /locus_tag="PKI65_08135"
     CDS             complement(764..1411)
                     /locus_tag="PKI65_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOB78436.1"
                     /translation="MDRVNIGWSKDDMAKLVANDIPDGSYVNLGIGIPELVANYIPEG
                     REIIIHTENGLLGVGPAPAPGEEDFDLINAGKKPITTIKGACFFHHADSFAMIRGGHL
                     DFTVLGAYQVSQNGDLANWSTGSPNDIPAVGGAMDLVQGVKNVFVIMSHTTKNGTPKI
                     VKKCTYPLTGVAVVSRIYTDLAIIDVTNKGLKVIKLAPGIDKDTLINKTEADLIL"
     gene            complement(1399..2118)
                     /locus_tag="PKI65_08140"
     CDS             complement(1399..2118)
                     /locus_tag="PKI65_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HOB78437.1"
                     /translation="MINKIFLTPQEAVADIFDGATIMIGGFGEAGSPIELIHALIDQG
                     AKDLTIISNNAGSGHVGLAALLEHKRVKKIICSYPKTSFSEVYPELYCSGLIEVELVP
                     QGTLAERIRCGGAGIPAFYTPTSVGTSLAEGKELREIDGELYVLEYALKSDFSLVKAY
                     QGDRYGNLIYYKTARNFGPIMCMAAKVTIVQVNKIVELGEIDPEAVVTPNIFVQRVVE
                     VPNPLHEHVLVSQNKKYPWTE"
     gene            complement(2131..3711)
                     /locus_tag="PKI65_08145"
     CDS             complement(2131..3711)
                     /locus_tag="PKI65_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78438.1"
                     /translation="MRVEKILFSEEELNNLMDRYHFSTEMIEIVKNENLLPLGVLSRL
                     QTAYPQYSLMKKPNKVDFYEKERWVGLKGFREIVDKLAKHGIIIDRIEEREVFIYVYR
                     FMVTAHVLNLIDWDNYQNDPLFYLTFPQPDMVREHARQKILAASTDEEREAIIKEYIN
                     ETNPHDGKQKLNKPTYINENGELELLDGLQHKYAPVVLILDEQTQNCFGFCTYCFRHA
                     QVRGDEDMFAQRSVGQVIGYLKRHKEITDILITGGDAGYITPARYREYIIPIIEDPEL
                     SHIRTVRLGTRMLSYYPEKILTHEYNEMLDLFKMLYDNGVQPMIVSHFSTPREIMNIT
                     TIAAIRRLKKYGVNLKSQSPIMNHISMYEDENGNIDVDRSAQNWIDLANIFAMLGVGF
                     HAMYIPRPTGEVEYFTRPLADINKVFNKIFKNLASINRPSRYITMTLSAGKISLLGDT
                     LIDNDRVFVLKFNEGRDMNWCDTVFLTEYDEFNSNMADLKPYKADKFFYEDELKEIEN
                     RLEKNILEAQKHHKIAER"
BASE COUNT         1342 a          679 c          519 g         1422 t
ORIGIN      
        1 gtcatcaact agtttttctt ggcttacagg agagaaatta tcactcacaa tatcttggct
       61 accacataat gggcaatttc taattacatc atcaatatta ggattattaa aataacatga
      121 gcacgtatta caatgatacc aagggctatc ataataaaca ttgccaccat ttattttaat
      181 ttctctaccc tcagcaaaag ccttagcgat tttttgtcga gcagaagcat aaatacgagt
      241 aaatgtagga cgagaaatat tcattatttc tgaggcttgt aaatgagtgt acattttata
      301 atcacataat ctgatggctt catactcttc atataatagt attattggtt tttcattttg
      361 tttggtgtta gttacaccat atggtttaaa tccctttaca acagggagtt ctattatttt
      421 tctcaatctt ttaattcttg ctgccatata ataaacattt gttcattgca aaattaataa
      481 acatttgttt aaaaaccaaa ttttttcaat atttttttta aaaaatattt taaaatgaat
      541 taaaatacag tatcaaaatc gtatttatta tctttgatag attttaataa aacataacaa
      601 aatcaatcat aaaactattt tttgcttatc tgtatataaa tgtgtataat attttaatta
      661 cctaaaataa aatactatat ttagattaaa tcaataatat attatataaa tttggcaaaa
      721 tcaataataa atttataaaa ttaaatgaat agagaaaaat atattataaa attaagtcgg
      781 cttcagtttt atttattaga gtatctttat caatgcctgg agccaatttt ataactttta
      841 gacctttatt agtaacatca ataatagcta aatcagtgta aatgcgagat actactgcga
      901 cacctgttaa agggtaagta catttcttaa caattttagg cgtaccattt ttagtagtgt
      961 ggctcataat cacaaaaaca tttttaacgc cttgcactag atccatagca ccaccaacag
     1021 cagggatatc atttgggcta ccagtagacc aattagctaa atctccattt tgtgaaactt
     1081 gataggctcc caaaacggta aaatccagat gccctccacg aatcatagca aagctatctg
     1141 catgatgaaa aaaacaagct cctttgattg tggtaatagg ttttttacct gcattaataa
     1201 gatcaaaatc ttcttctcct ggtgctggtg ctggacctac gcctaataga ccgttttctg
     1261 tatgtataat aatttctcta ccttctggta tgtaattagc cactaattct ggaatgccta
     1321 tacctagatt tacataagag ccatcaggaa tatcattggc aactaatttt gccatatcat
     1381 ccttgctcca tccaatattt actctgtcca tggatatttt ttattttgac taactaaaac
     1441 atgttcgtga aggggatttg gtacttctac gactctctgc acaaaaatat taggagtaac
     1501 aacggcctcg ggatctattt cgcccagctc aactatttta ttcacttgaa caatagttac
     1561 tttagcagcc atacacataa taggaccaaa atttcttgca gttttataat aaatgagatt
     1621 gccatatcta tctccctgat aggcttttac aagtgaaaaa tcagatttta atgcatattc
     1681 taatacgtat aattcaccat caatttctct caattcttta ccttctgcta atgaagtacc
     1741 tacactagta ggagtataaa aagctgggat gccagcacca ccacagcgga tacgttctgc
     1801 taatgtacct tgcggtacaa gctctacctc tatcagtcca ctgcagtaca attctgggta
     1861 aacttccgaa aaagaagttt taggatatga gcaaataatt ttttttactc gtttatgttc
     1921 taataaagca gctaatccca catgtccact accagcatta ttactaataa ttgttaaatc
     1981 ttttgctcct tgatctatta atgcgtgaat taattcaatg gggctaccag cctcgccaaa
     2041 tccaccaatc attatagtag ctccatcgaa aatatcagct actgcctctt gaggggttaa
     2101 aaaaatttta tttatcataa tttaatagta ttatctttca gctattttgt gatgtttttg
     2161 agcttctaaa atattttttt ctaatctatt ttctatttct tttaattcat cctcataaaa
     2221 aaatttatct gctttatatg gtttaagatc agccatatta ctattaaact catcatattc
     2281 agttaaaaat acagtatcac accaattcat atctctacct tcattaaatt taagaacaaa
     2341 aactctatca ttatctatta gagtatcacc gaggagagaa attttaccag cagatagagt
     2401 catggtaata tagcgagaag gacgattaat agaagctaaa tttttgaaaa ttttattaaa
     2461 tactttattt atatcagcaa gtggacgtgt gaaatattct acttcaccag taggacgtgg
     2521 tatatacata gcgtgaaaac caacacctaa catagcaaaa atatttgcta aatctatcca
     2581 attttgagct gagcgatcta catcaatatt accattttca tcttcataca tactgatatg
     2641 attcattata ggactttgac tttttagatt tactccatat ttttttaatc tacgaatggc
     2701 agcaatagtg gtgatattca ttatctctct tggtgtagaa aaatgagaaa ctatcatagg
     2761 ttgtacaccg ttgtcatata gcattttgaa taaatcaagc atttcattat attcatgagt
     2821 taaaattttt tcaggataat aactaagcat gcgagtccca agtctgacag ttctaatgtg
     2881 tgatagttca ggatcctcaa taataggtat tatatattct ctatacctag ccggtgttat
     2941 atatccagcg tctccacctg ttatcaatat atcagtaatc tctttatgtc tttttaaata
     3001 accaattact tgccctacac ttctctgagc aaacatatct tcatcgcctc tcacttgagc
     3061 atgacggaag caataggtac aaaaaccaaa acaattttga gtttgttcat cgagtatcaa
     3121 aactactgga gcatatttat gctgtagacc atctagaagc tctaattctc cattttcatt
     3181 gatatatgta ggtttattca atttttgttt accatcatgt ggattagttt cattgatata
     3241 ttctttaatt attgcttctc tttcttcatc tgttgaggca gctaatattt tttgcctagc
     3301 atgctcacgc accatatctg gctgtggaaa tgtaagataa aataaaggat cattttgata
     3361 attgtcccaa tcaattaaat tgagcacatg agcagttacc atgaatctat aaacataaat
     3421 aaacacttct ctttcttcaa ttctatcaat aataatccca tgcttagcta atttatctac
     3481 aatttctcta aatcccttta agcctaccca acgctctttt tcataaaaat ctactttgtt
     3541 tggttttttc attaaagagt attgaggata tgcagtctgc aatctcgata aaacccctaa
     3601 tggcaaaaga ttttcattct ttactatttc tatcatttca gtactgaaat gatagcgatc
     3661 cattaaatta tttaattctt cttctgaaaa taagattttt tctacacgca ttttatttta
     3721 ttatttataa tgcaaatata aaaataaatt ttaataaata ttaaaattta ctaataaaat
     3781 tacaaataat tttaaaaaag tataaaaaac ataataataa tatgaattat gtatataaaa
     3841 attttataat aagcttaaat gtgtagtcat ttaaggagcg agagatggga agagtataga
     3901 aaattcatat cctttgtttt actttacgta gtttatacta ccatcaaata atatttattt
     3961 aa
//
LOCUS       DAOPRN010000103         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_18348_length_3945_cov_98.429306, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000103 DAOPRN010000000
VERSION     DAOPRN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18348_length_3945_cov_98.429306"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PKI65_08150"
     CDS             <1..816
                     /locus_tag="PKI65_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOB78439.1"
                     /translation="QYGGYFISYGYAIEERKKYILEKYPPVLNYILYSTDFILKRVMP
                     KLPICKECYFAITKGKNRAISKAEILGRLYFCGFRVINTTLIDNSFYFIAQKINIPKQ
                     DPHPTYGPLIKLKRIGKDGKIINVYKFRTMHPYSEYLQSYIYERNKLQEGGKIANDFR
                     VSTIGHFMRKFWIDELPMFINLFRRQLKIVGVRPLSLQYFKLYPTEFQEIRIKYKPGL
                     IPPFYVDLPSNLDEIIESEKKYLASYDEHPFRTDINYFFKAVYNILFKKARSK"
     gene            complement(1115..2101)
                     /gene="rfbB"
                     /locus_tag="PKI65_08155"
     CDS             complement(1115..2101)
                     /gene="rfbB"
                     /locus_tag="PKI65_08155"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOB78440.1"
                     /translation="MDNIIITGGAGFIGSHLVRRLVKKYPDYNIINLDKLTYAGNLDN
                     VKDVEHKPNYSFIKADIVDFDIIYEIFKKYNPIAIFHLAAESHVDRSIVNPMEFVYTN
                     VIGTVNLLNAAKAVWGEDFQNRRFIHVSTDEVYGSLGEMGYFTEETPYSPRSPYSASK
                     ASSDHFVMAYYHTYGLPTMITNCSNNYGPNQYPEKLLPVIINNIYHEKPLPVYGQGIN
                     VRDWLYVEDHCEALELVWQKGMIGESYNIGGNNEWRNIDLVRLICDIMDKKLNRPVGY
                     SEKLITFVKDRPGHDLRYAIDSKKISNELGWKPRTDFKEGLSKTIDWYLERM"
     gene            complement(2083..3123)
                     /gene="galE"
                     /locus_tag="PKI65_08160"
     CDS             complement(2083..3123)
                     /gene="galE"
                     /locus_tag="PKI65_08160"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOB78441.1"
                     /translation="MILVTGGAGYIGSHVIAALLQSGYEVVNVDDFSRSHPIIHKNLE
                     EHFGIDIPLYQIDLADARAFKELMKIKEPIDAVIHLAAYKFVEESYEDPALYYNNNIP
                     ILLHLLDYVHQVDVQYFIFSSSCTVYGEPDEIPVTEKSPLKEPVSPYGHTKQICEYIL
                     KNYAVAYRKMKVVSLRYFNPIGAHPDIMIGEIPTGQSKGLLPYLWQSVDKTLPELTIY
                     GKDYPTHDGTPMRDYIHIVDLAQAHVASLDQLFAEKINSSYEVINVGLGKGYTVLEVI
                     NTFEKVVGLDVPHKFGNRRKGDISAIYTDNKKMKELLGFVPKYNLEDMLVHSWLWYNN
                     YKNHIKPWIISL"
     gene            complement(3312..>3945)
                     /locus_tag="PKI65_08165"
     CDS             complement(3312..>3945)
                     /locus_tag="PKI65_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOB78442.1"
                     /translation="NFSPWAITRNDHPLPISLLYFNGDCKDSIVELNWATASEINNDY
                     FTIEYTNSLNDEDVMWTPIGIVNGAGNSNTMQTYQFIDKPILRDINAEIIYYRLKQTD
                     YNGNYEYFSSIYVDCRIDNYINTEIINAYCEQKNNCNVVTVLFKMPGEINYTIKLYNV
                     FGQLLATTKGISNEGENITQLYINEPLKESVYIVILHDNFQLKTKKVLLY"
BASE COUNT         1337 a          665 c          505 g         1438 t
ORIGIN      
        1 caatatggag gctatttcat tagctatggc tatgctatag aggaaaggaa aaaatatatt
       61 ttagaaaaat atcctcctgt tctcaattat attttgtaca gcacagattt tattttaaaa
      121 agggtaatgc ccaagcttcc tatttgtaaa gaatgctact ttgccataac taaaggtaaa
      181 aatcgtgcaa tatcaaaagc agagatttta ggtagattat atttttgtgg ttttagagta
      241 atcaatacta ctttaataga taatagtttt tatttcattg cacaaaaaat taatattcct
      301 aaacaagacc cacaccctac ttatggtcca ttaataaaac taaaaagaat aggcaaagat
      361 ggtaaaatca taaatgttta taaattcagg actatgcatc catactcaga gtatctacaa
      421 agctatattt atgagcgaaa caaactacag gaaggtggta aaattgctaa tgatttcaga
      481 gtttctacaa ttggacattt tatgcgtaaa ttttggatag atgagctacc tatgtttata
      541 aatcttttta gaagacaact aaaaatagta ggcgtcagac ctctgagcct tcaatatttt
      601 aaattatatc ctacagaatt tcaagaaata cgcattaaat ataaaccagg attaatacct
      661 ccattttatg tggatttacc tagtaactta gatgaaatta tcgaatctga gaaaaaatat
      721 ttagcatcat atgatgagca cccttttcgt acagatataa attatttttt taaagctgtt
      781 tataatatat tattcaaaaa agcaagatct aaataattga taaaaagctc aaattattag
      841 taaaactcat aatgagtatc cttttatgtt atttactaga ccatttaata attataaaaa
      901 aaacattcta gcaaattttt atagaaaatt ataaatataa aagttcaata ttaactttta
      961 gatgcttttt taataattat tctaaataca gatggctgtg attgtttatt aataatcttt
     1021 ctattctctt cttttatcta tctccacttt gtcaatctat cccttcgcca tatattttct
     1081 tttctccttt tatcccattt accatattat atactcacat tctctccaga taccaatcaa
     1141 tagttttaga taatccttct ttaaaatcag ttctaggctt ccagccaagt tcgttgctaa
     1201 tttttttact atctatagca tagcgtaaat cgtgacctgg tctatctttt acaaaggtta
     1261 ttagtttctc actatatcca acaggtctat ttaacttttt atccattata tcacatatta
     1321 gacgaaccaa atctatatta cgccattcat tattgccgcc tatattatat gattcaccta
     1381 tcataccttt ttgccaaacg agctctagag cttcacaatg atcctctaca tataaccaat
     1441 ctctcacatt aataccctga ccatacacag gtaatggttt ttcatgataa atattgttaa
     1501 ttatcactgg taagagtttt tcaggatatt gattggggcc ataattatta gaacaattag
     1561 tgatcatcgt aggcaagcca taagtatggt aataagccat tacgaaatgg tcactagaag
     1621 ccttagatgc ggagtaggga ctacgaggcg aataaggtgt ctcttcagtg aaatatccca
     1681 tttctcctaa tgaaccatat acttcgtcag tagacacatg tataaatcgt ctattttgaa
     1741 aatcctcacc ccatacagct tttgctgcat ttaatagatt cacggtgcct atcacatttg
     1801 tataaacaaa ttccattgga tttactatag atctatctac atgactctca gcagctaaat
     1861 gaaaaatagc tatggggtta tattttttaa aaatttcata tataatatcg aaatctacaa
     1921 tatctgcttt tataaaagaa taattgggct tatgctccac atctttcaca ttatccagat
     1981 ttccagcata agtcaattta tccaaattaa taatattata atcaggatat ttttttacta
     2041 atctacgtac aagatgggat ccaatgaatc cagcacctcc tgttataatg atattatcca
     2101 tggttttata tgatttttat aattattata ccacagccag ctatgcacaa gcatatcctc
     2161 taaattatat ttagggacaa atccaagcaa ttccttcatt tttttattat cagtatagat
     2221 agctgagata tctcccttcc ttctattacc aaatttatgt ggcacatcga gtcctaccac
     2281 tttttcaaat gtatttatga cttctaatac agtataacct ttacctaatc ctacatttat
     2341 aacttcatat gaagaattta ttttttctgc aaaaagttga tccaatgaag ctacatgtgc
     2401 ctgagctaag tctactatat gtatgtaatc tctcatagga gtgccgtcat gcgtaggata
     2461 atctttgcca taaatcgtaa gttcgggtaa agttttgtct acgctctgcc atagatatgg
     2521 caagagacct tttgattgac ctgtaggtat ctctcctatc attatatcgg gatgtgcacc
     2581 gataggattg aaataacgaa gcgataccac tttcattttt ctatatgcta cagcataatt
     2641 ctttaatatg tattcgcata tttgtttagt atgtccataa gggctaacag gctcctttaa
     2701 tggtgatttt tctgtaacgg gtatctcatc aggctctccg tatacggtgc atgaagacga
     2761 aaaaataaaa tattgtacat caacctgatg tacatagtct aataaatgca gcaaaatagg
     2821 aatattatta ttgtaataca aggcaggatc ctcataagac tcttctacaa atttataagc
     2881 tgccaaatga attacagcat ctataggttc ctttattttc atcaattctt taaaagctct
     2941 cgcatctgct agatctatct gatataatgg tatatctata ccgaaatgct cttctagatt
     3001 tttatgaata ataggatgtg aacgagaaaa atcatctacg ttcaccactt cataaccact
     3061 ttgtaacaat gctgctatta catgagaacc tatatatcct gcccctcccg ttactaatat
     3121 cattgaattt ttatttttca attttatgca aatttcgaca ttttttttaa attaattgag
     3181 gatttttatt atttaaatga aaaatgacaa attttatatc ctaattatat aatttttcaa
     3241 atttttttgt caaaaaagtt catgcatctt attgcataaa aaagataatt aattataaag
     3301 cataaaaaaa cttaatataa aagtactttt ttagttttta attgaaaatt atcatgcaat
     3361 attacaatat atactgattc ttttaaaggt tcatttatat atagttgagt tatgttttca
     3421 ccctcattac ttattccttt agtagtggca agcaattgtc caaatacatt ataaagtttt
     3481 atcgtataat ttatctcgcc aggcattttg aataatactg tcacaacatt acaattattc
     3541 ttttgttcac aataagcatt tatgatttct gtatttatat aattatctat tctgcaatca
     3601 acataaatag aactaaaata ttcataatta ccattataat ctgtttgctt tagcctataa
     3661 taaataattt ctgcatttat atctcttaaa ataggtttat caataaattg ataagtttgc
     3721 atagtgttgc tattaccagc tccatttact atacctattg gagtccacat cacgtcttca
     3781 tcatttaagc tattagtata ttctattgtg aagtaatcat tattaatttc cgaagctgta
     3841 gcccagttta gctctacaat actatcctta caatctccat taaaatataa taaacttatt
     3901 ggtaaaggat gatcatttct tgtaatggcc catggtgaaa aatta
//
LOCUS       DAOPRN010000104         3729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_19776_length_3729_cov_214.706042, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000104 DAOPRN010000000
VERSION     DAOPRN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19776_length_3729_cov_214.706042"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PKI65_08170"
     CDS             <1..260
                     /locus_tag="PKI65_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78443.1"
                     /translation="NKTQIRRGVYSVQVVDKHINDFKVWLRRFRGVATKYLQSYLMWF
                     VITHKYLTSEIESDIGRLLNLASSDRWAWDEYWRLVNTKYR"
     gene            541..723
                     /locus_tag="PKI65_08175"
     CDS             541..723
                     /locus_tag="PKI65_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78444.1"
                     /translation="MRRFRGVATKYLQSYLMWFVITHKYLTSEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
     gene            complement(730..864)
                     /locus_tag="PKI65_08180"
     CDS             complement(730..864)
                     /locus_tag="PKI65_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78445.1"
                     /translation="MKRNFYKAKFSGSIYLNWDRNYRRENYRNCEGSERCLEANHCFT
                     "
     gene            1003..1185
                     /locus_tag="PKI65_08185"
     CDS             1003..1185
                     /locus_tag="PKI65_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78446.1"
                     /translation="MRRFRGVATKYLQSYLMWFVITHKYLISEIESDIGRLLNLASSD
                     RWAWDEYWRLVNTKYR"
     gene            1598..>3729
                     /locus_tag="PKI65_08190"
     CDS             1598..>3729
                     /locus_tag="PKI65_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOB78447.1"
                     /translation="MLLSKKPIEKSLNKAFLKQDLTINQMDIFITNLKILLDEIDQLI
                     FHDQDEENFKTPIINFLKNTYYKDNYYINSSKKYDLIIGNGPKLSDHIAVIIETKRPS
                     NTAEMIDDSVPNAKALHELVHYYMQERLNDNDEIKYLIATNCYKWYIFDAVDFENLFF
                     KNNDFKSNYKAWNSQQTVDSTTKSIYEKIKDFIDNNIDVLEATYFDLKDYKKYINSTN
                     VEDLENLISLYKILSPEHLLKKPFANDSNTLNKEFYNELLYIIGLEEKIKNGKIIIDR
                     KSNKNYGSLIENTINILITRNKLKQIEDIEQYGDNVDEQIFSIALELCITWLNRILFL
                     KLLEGQLIKYHNGDTKYAFLSIDKVKDFDTLDELFFEVFAVKHQDRSPRIKEKYEHIP
                     YLNSSLFEINDLEHQTISIANLKDDLTIPIYSNSVLKNTIKKNELNTLDYLLIFLSSY
                     NFSSDSHAKIQKDNKTIINAAVLGLIFEKINGYRDGSYFTPGFITMYISRQTIRRAVV
                     KKFRENENIDFDSFDDVKNYCKIHHKTEDIRRFNSYINSIKICDPAVGSGHFLVSALN
                     ELIAIKSELNILADDGILIDCEVSVDNDELIVINKYNKPYEYYLIDGIKPSNEAQQIQ
                     RILFNEKAQLIENCLFGVDINTKSVLICRLRLWIELLKNAYYKAPNYKELETLPNIDI
                     NIKCGDSLISRFNLKDDTQNLTPADRGR"
BASE COUNT         1400 a          537 c          594 g         1198 t
ORIGIN      
        1 taaacaaaac gcagattaga cgcggtgtat acagtgtaca agtagtcgat aagcacataa
       61 atgatttcaa ggtttggttg cgacgattta gaggagtggc tactaaatac ttacagagct
      121 atttgatgtg gtttgtaatc acacataaat acttgacaag tgagatagaa tcagacattg
      181 gtcggctttt aaacctcgca tcctcggaca gatgggcgtg ggatgagtat tggcggctgg
      241 tgaatacaaa gtatagataa actacttcaa gtaaaacaat gatttgcttc tcgacccttt
      301 tcgattcctt tcacaattac aataatttcc ccttctataa tctctattcc aatttaaata
      361 aatagatcca ggaaatttag ctttgtaaag attacgcttc ataaaggcga tttgtgaacg
      421 gagctactta aaaatatctt ctgttttact tgaagtagtt tatagcataa ttaaataatt
      481 agacgcggtg tatacagtgt acaggtagtc gataagcatg taaatgattt caaggtttgg
      541 ttgcgacgat ttagaggagt ggctaccaaa tacttacaga gctatttgat gtggtttgta
      601 atcacacata aatacttgac aagtgagata gaatcagaca ttggtcggct tttaaacctc
      661 gcatcctcgg acagatgggc gtgggatgag tattggcggc tggtgaatac gaaatataga
      721 taaactactt caagtaaaac aatgatttgc ttctaaacac ctttcgcttc cctcacaatt
      781 acgataattt tcccttctat aatttctatc ccaatttaaa taaatagatc cagaaaattt
      841 agctttgtaa aaattacgct tcataaaggc gatttgtgaa cggagctact taaaaatatc
      901 ctttgtttta ctttacgtat tttatagcat aattaaataa tttgacttgg tatatacagt
      961 gtacaggtag tcgataagca tataaacgat ttcaaggttt ggttgcggcg atttagagga
     1021 gtggctacta aatacctgca gagctatctg atgtggtttg taatcacaca taaatactta
     1081 ataagtgaga tagaatcaga cattggtcgg cttttaaacc tcgcatcctc ggacagatgg
     1141 gcgtgggatg agtattggcg gctggtgaat acaaagtata gataaactac ttcaagtaaa
     1201 acaatgattt gtttctagac ccctttcgat aataaatttg ttaataaata ttaacctaaa
     1261 taattatttt tttgtaacaa tctaaaattt tattacccac aataaacgtt atagaacctc
     1321 aaatatcttt gtcttgcaac caacttatgg tagatagaat tattattttt tcgtgtaaat
     1381 aaaaacaata gctttgaatt ttgaatattg aaacatattt tcataaatca caacacattt
     1441 attaagatag agtcaagtga tcaaaaatct gagagcatcg tacactaact gcttagtatt
     1501 tttataatct atgatttatc tactattagt catagttttt atgttcaaaa atttaattat
     1561 tagattaaaa ttaaatatat ttgtaaaaaa agtttacatg ctattgagca aaaaacctat
     1621 tgaaaagagt ctgaataaag cgtttctaaa gcaagatctc actattaatc aaatggacat
     1681 ttttataacc aatctaaaaa tattattaga cgaaatagat caattaatct ttcatgatca
     1741 ggatgaggaa aactttaaaa cacctataat taattttcta aaaaacactt actacaagga
     1801 taattattac attaatagtt ctaaaaaata tgatctaata ataggaaatg gacctaaact
     1861 atctgaccat atagctgtaa taatagaaac taaaagacct agcaatacag cagaaatgat
     1921 agatgatagc gtacctaatg ctaaggctct ccatgagctt gtccactatt atatgcagga
     1981 aagactaaat gataatgatg aaattaaata cttgatagct actaattgct ataaatggta
     2041 tatattcgat gcggtagatt tcgaaaattt gtttttcaaa aataatgatt tcaaaagtaa
     2101 ttataaagca tggaactctc aacaaactgt tgatagtact actaaatcta tttatgaaaa
     2161 aataaaagat ttcatagaca ataatataga tgtattagaa gctacatatt tcgatttgaa
     2221 agattataaa aaatatatta atagcacaaa tgtagaagat ttagaaaatc tgataagttt
     2281 atacaaaatc ctttcgcctg agcatctgct caaaaaacct tttgctaatg acagtaatac
     2341 tctaaataag gaattttaca atgagttgct atacattata gggctagaag aaaaaataaa
     2401 aaatggtaaa attattattg atcgcaaaag taataagaac tatggtagct tgatcgaaaa
     2461 cactataaat attttaataa cgagaaataa actgaaacaa atagaagata tagagcaata
     2521 tggcgacaac gttgatgagc aaatttttag tatagcatta gaattatgta ttacttggct
     2581 caataggata ctttttctaa aacttttaga aggacaattg ataaaatacc acaatggaga
     2641 caccaaatat gcttttttat cgatcgataa ggtaaaagat tttgatactc tagatgagtt
     2701 attctttgaa gtcttcgcag tgaaacatca agacaggtca cctagaatta aagaaaaata
     2761 tgaacatata ccttatttga atagttcgct attcgaaatt aatgatctag aacatcaaac
     2821 tatctccatt gctaatctga aagatgatct caccatacct atttattcta attctgtttt
     2881 aaaaaatact ataaagaaaa atgaactcaa tactttagat tatctactaa tatttttatc
     2941 atcatacaat ttttctagtg atagtcatgc taaaatacaa aaggataata agactataat
     3001 aaatgcagca gtactaggct tgatctttga aaaaattaat ggctatcgcg atggttcgta
     3061 ctttactcca ggttttatta ctatgtatat aagcagacaa accatacgta gagccgttgt
     3121 gaaaaaattt agagaaaatg aaaatataga ttttgatagc ttcgatgacg taaaaaatta
     3181 ttgcaaaata catcataaaa ctgaagacat tagacgtttt aattcttata ttaattctat
     3241 aaaaatttgt gaccctgctg taggctcagg acatttcctt gtctctgctc tcaacgaatt
     3301 aatagctatc aaaagtgaat taaatatatt ggcagatgat gggattttaa tagattgcga
     3361 agtcagtgtg gataatgacg agctaatagt gattaataaa tataataaac catatgaata
     3421 ttatttgata gatggcatca aaccctctaa tgaagctcaa cagattcaaa ggattttatt
     3481 taatgaaaaa gctcaattaa tagaaaactg tctcttcggt gtagatatca atactaaatc
     3541 tgttcttatt tgtagattgc gcctatggat agaactttta aaaaatgctt attacaaagc
     3601 tcccaattat aaagaattag aaactctacc taatattgat attaatatca aatgtggcga
     3661 ctcgcttatt agcagattta atctgaaaga tgacactcaa aacctcacac ccgctgatag
     3721 gggaagatt
//
LOCUS       DAOPRN010000105         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_20054_length_3685_cov_115.741873, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000105 DAOPRN010000000
VERSION     DAOPRN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20054_length_3685_cov_115.741873"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..3234)
                     /locus_tag="PKI65_08195"
     CDS             complement(172..3234)
                     /locus_tag="PKI65_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOB78448.1"
                     /translation="MSITINEAKEKIKELVDKFDFNIQQYKNKNYDEANTRVDFIDPF
                     FEALGWDVSNKAGYAEQYREVVREDKIKIQGKTKAPDYCFRIGGNRKFFVEAKKPQVD
                     IKSDYNPAYQLRRYAYTANLPLSILTDFEEFAIYDTRIKPNVNDKADNARIFYCTYRE
                     YLKNFDFIYNTFSKEGILKGSFDRYIESNKNKKGTSEVDKEFLKLIDDWREKIAKNIA
                     KKNYNLDIDEINFAVQKIIDRIIFLRIAEDRGIEKYGQLQDLLNKPYIYSELLKLFYD
                     SENKYDSGLFDFNKEDKITQNILIDDDILKYIIKSIYYPDSPYEFSVLDVEILGNIYE
                     QFLGKTIRLTPSRQVKIEYKPDVKKAGGVYYTPSYIVDYIVSNTVGELIKDKTPEDVN
                     NLKMLDPACGSGSFLLGAYEFLLNYHLNYYLKNKDFAIKANKIYEKGHNDYSLTFDEK
                     KKILLNNIYGVDIDSGAVEVTKLSLILKLLEGETQELIKNLKNHILPDLSNNIKCGNS
                     LIDTSVLSSQTLSIFDPDNLKHLNPMNWEDAFPEIIKNGGFDVIIGNPPYVKEYTDRE
                     PFEYMKLCKAKEYYQGKMDLWYAFACKSIDLLKAGGYHSFIATNNWITSQGASILRNK
                     ILDETVIIKFIDFADFKVFQNASIQTMIYVVKKEKPNKKYKVKYTKILNKNILLTDLV
                     DRLNKDISKEKYINTEDYEWFYAIINPNELKNNTFTFVSDKIQAILDKIKIKSNYKLI
                     ENVASTGIDVHQDFVIDSHLKLLKDKEIKKGDGIFILSEAEKNSLNLTNDELEIIKPY
                     YTTKELKRYYGSNKNKYWIIYSDINVRKNINKYPNIKRHLDKYKPIITSDFKPYGLHR
                     ARDQRFFEGEKIISLRKTRIPNFTYTDFSCYVSQTFFVIKPEDIDMKYLTGLLNSKVI
                     YFWLFFKGKKQGDQLQIDKEPLLQIPLIKPEDENIIKYIAKLVDDIIQISQTLENCQL
                     PNQINFYQNLIKSRENEIDQKIYELYNLSDDDKMEIEKLWGNNVVCEK"
BASE COUNT         1190 a          515 c          414 g         1566 t
ORIGIN      
        1 acatttaatt ttttcatatt tttatgtttt taattggttt gtgttgcaaa attaacaaat
       61 tcatttaata aattatcctt taattttttt ttaactttta cattataata agtgagtgat
      121 agtactagca tacataaatt attgttgaag cttcgattac aatcgtcgct tctatttctc
      181 gcatactaca ttattacccc acagtttttc tatctccatt ttatcatcat cagataaatt
      241 gtacagttcg tatatttttt gatcaatttc attttctcgg cttttgatta ggttttgata
      301 aaagtttatc tggttaggta gctgacaatt ttcgagagtt tgacttattt gtataatgtc
      361 atctactagt tttgcaatat attttattat attttcgtct tctggtttga ttaatggtat
      421 ttgcaaaagt ggttctttat caatttgtag ttgatctcct tgctttttcc ccttaaagaa
      481 gagccagaaa taaataacct ttgaatttaa caagccggtt agatatttca tgtctatatc
      541 ctctggttta attacaaaaa aagtttgtga tacgtaacac gagaaatctg tgtatgtgaa
      601 attaggaatc ctggttttac gcaatgatat tattttttca ccttcaaaaa atcgctgatc
      661 tcttgctcta tgcagaccat aaggtttgaa atctgatgta ataatcggtt tatatttatc
      721 caaatgcctc tttatatttg gatatttatt tatatttttt cgcacattta tatcagaata
      781 tattatccaa tatttgtttt tatttgatcc gtaatacctt tttagttctt tggtagtgta
      841 atatggtttt atgatttcta actcatcatt tgtcaaattt aatgaattct tttcagcttc
      901 tgataaaata aatattccgt cacctttttt tatttcctta tcttttaata attttaaatg
      961 actatctata acaaaatctt ggtgtacatc aatgccagta gaagcaacat tttcaattaa
     1021 tttataatta gattttattt ttattttatc taaaattgct tggattttat cagaaacaaa
     1081 tgtaaatgta ttatttttta attcattggg atttataata gcatagaacc attcataatc
     1141 ttcagtattt atgtattttt ctttactaat gtctttgttt agcctatcta ctaaatcagt
     1201 taaaagtata tttttgttta atattttagt atatttcact ttatattttt tattaggttt
     1261 ttcttttttt acaacataaa tcattgtttg aatagatgca ttttgaaaaa ctttgaagtc
     1321 tgcaaaatca atgaatttta ttattacagt ttcatctaag attttatttc tcagtattga
     1381 tgctccttgc gatgttatcc agttgttagt agcgatgaag ctatgatatc caccagcttt
     1441 tagtagatct atagatttac aagcaaatgc ataccagaga tccattttac cttgataata
     1501 ttcttttgcc ttgcataact tcatatattc aaaaggttct ctatcagtat attcttttac
     1561 ataaggagga ttgcctatta ttacatcaaa tccaccattc ttaatgattt ctggaaaggc
     1621 atcttcccaa ttcataggat ttaaatgctt caaattgtca ggatcaaaaa ttgaaagagt
     1681 ctgactgctt aaaactgatg tatctatcaa tgaattacca catttaatat tatttgataa
     1741 atctggcaga atatgatttt ttaaattttt tatcagctct tgtgtttcgc cttctaataa
     1801 ttttaatatt aaagacaatt tggttacttc taccgcaccg gaatctatat ctacgccata
     1861 aatattgttt aataaaattt tctttttttc atcaaaagtt agactataat cattatgtcc
     1921 tttttcatat atcttatttg cttttatggc aaaatcttta ttttttaggt aataatttaa
     1981 atgataattt aaaagaaatt catatgctcc taataaaaaa ctaccagaac cacatgcagg
     2041 atctaacatt tttaaattat ttacatcttc tggagtttta tcttttatta attcacctac
     2101 agtattacta acgatataat caacaatata gcttggagta taataaacgc caccagcttt
     2161 tttcacatca ggtttatatt ctatttttac ctgtctagaa ggcgttaatc ttatagtttt
     2221 accaaggaat tgttcataaa tattacctaa aatctctaca tctaacactg aaaattcata
     2281 gggtgaatct ggatagtaaa tagattttat tatatacttt aaaatatcat cgtctattaa
     2341 tatattttgt gtgattttat cttctttatt aaaatcaaac aacccactat catacttatt
     2401 ctctgaatca taaaataatt ttaataattc ggaataaata tatggtttat ttaataaatc
     2461 ttgtaattga ccatattttt ctatacctct atcttctgct atccttaaaa aaattattct
     2521 atcaatgatt ttttgtacag caaagttgat ctcatctata tctaaattat aatttttttt
     2581 agctatattt ttagctattt tttctctcca gtcatctatt aattttagga attctttatc
     2641 aacctcagaa gtgccttttt tatttttatt tgactcaata tatctatcaa aactaccttt
     2701 tagaatgcct tctttggaaa aagtattata aataaagtca aaattcttaa ggtattccct
     2761 atatgtacaa taaaatattc tagcattgtc tgctttatca tttacatttg gttttattct
     2821 agtatcataa atagcaaatt cttcaaaatc agttaaaata gataaaggca aattagctgt
     2881 gtatgcataa cgtcttaatt gatatgcagg attataatct gatttgatat ctacttgtgg
     2941 ttttttagct tctacaaaaa attttctatt cccaccaatg cgaaagcaat aatctggtgc
     3001 ttttgttttt ccttgtattt ttattttatc ttcccttact acctctctgt attgctctgc
     3061 ataaccagct ttattagaca cgtcccaccc caaagcctca aaaaatggat ctatgaaatc
     3121 tacccttgta ttcgcctcat catagttttt gtttttatat tgttgtatat taaaatcaaa
     3181 tttatcaact aattctttta ttttttcttt agcttcattt attgttatgc tcataatttt
     3241 atactttaga aagtaaaatt aattaaaaat taaattattc taacatcata tttttattat
     3301 ttacaatctc ttgtgatatt tcaggattat aaaaatttac atatttattc acaatggata
     3361 aatatatatt ctccataaat taaagcaaac tagatgattt tttttaaaaa aaaattattc
     3421 atttctacaa ttacttgatt tttttttatt actcggataa taatgataat aaaatcttat
     3481 aagatttgaa atatgtgata tttaaattgt ttataatatt attttttaag aaatgcaaag
     3541 taaattaatg aaattcattg ggatttgaga agggggttgg ggtaatttta attgttaaat
     3601 atgatattgg attaatatag aaataattgc taattttacc aaaatttata attatgaaaa
     3661 ccatattaaa agatcaagaa ttaga
//
LOCUS       DAOPRN010000106         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_20851_length_3576_cov_184.497586, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000106 DAOPRN010000000
VERSION     DAOPRN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20851_length_3576_cov_184.497586"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PKI65_08200"
     CDS             <1..389
                     /locus_tag="PKI65_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB78449.1"
                     /translation="EYYKAALRIQPNSEEALYMYAYHLQNTNRIDQAIEYYQKILTIN
                     PINKFAYYNLGYIYLVYKKDFNTSIYNFTKSIEIDSSYAEAYYNRGLAYEQLGENEKA
                     ISDYKTSLKYKTNFDKAIKRLNALQK"
     gene            812..1804
                     /locus_tag="PKI65_08205"
     CDS             812..1804
                     /locus_tag="PKI65_08205"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78450.1"
                     /translation="MEKLLKNSKEYLALPKDHKRDFLDQLYQFLIDDHATVVDYQKLK
                     IQSTAAICPNCKSENVIKAGTRKGRQIYRCKNCRYQFSETARTFVYRMRKYPLMLDYI
                     RCMLEGKSLRACADEVGICLKTSFRWRHRILAALRSFEGGISFSGIVEADELLMQYSE
                     KGRKYKSREEYEKAKKKRHPKVAVLVVTDREGNLLFKHVGDKKVKNEQLKKELNKRIT
                     ESNVLCVKPKKEFKKAVKDINSKKVIVNKKQLKRGLYSVEIVESKIDNFKKWLTRFHG
                     VATKYLQSYLMWFVVMNKYLKDIIEPDIGRLLNLSSHDRWAWDKYWKLMKLKYK"
     gene            2190..2357
                     /locus_tag="PKI65_08210"
     CDS             2190..2357
                     /locus_tag="PKI65_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOB78451.1"
                     /translation="MAYVITDDCTACGSCIDECPAEAISPGDIYKIDPELCTDCGSCA
                     EVCPVEAIHPE"
     gene            complement(2408..>3576)
                     /locus_tag="PKI65_08215"
     CDS             complement(2408..>3576)
                     /locus_tag="PKI65_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78452.1"
                     /translation="SNEIILRNIQLCDDVRAAINILKSFGKTINFNNENTIKISGQIN
                     NQLPEEIYCGESATLLRMLVPINHSLQNKGRYTGDKTLLNRDFGDLKDMLTPLGLQYS
                     SDISQCDIFKLPFSVFGNLSSQDNYYIKADKSSQAVSGLLISRSFNNAPSSISCSSLV
                     SAPYVYLTADIINMYGKIIEFNDKNFIITPQKSIELYKDFFIEGDWSLGAMILALGLT
                     IDSCEVKNLTAHSPQPDSKILKLFDNLSIRYQVNEKDQSIITHRQSYPGYTYDITDTP
                     DLVPALMILAANANSPSIINGICRLTNKETNRANTLSETFINLGGKLDVINDNNWIIY
                     PSQMHGGKIHSHNDHRVALLAIFLGLQSNEIVEIDDCECINKSYSAQFLKNLLK"
BASE COUNT         1309 a          480 c          574 g         1213 t
ORIGIN      
        1 atgaatatta caaagctgct ctgagaatac aacctaattc tgaggaagct ctttacatgt
       61 atgcatatca tttgcaaaat acaaatcgca tagaccaggc tatagaatat tatcaaaaaa
      121 ttcttactat aaatcctatt aataaatttg cttattataa tctaggatat atttatttag
      181 tttataaaaa agattttaat acatcaatct ataattttac caaatctata gaaatagact
      241 ctagctatgc agaagcatat tacaatagag ggttggcata tgaacaactc ggtgaaaatg
      301 aaaaagcaat atcagattac aagacaagtt taaaatacaa aactaatttt gacaaagcta
      361 ttaaaagatt aaatgcattg caaaagtgaa atgagctgag aaaaagctat agagaaaagt
      421 aaattattac atttgaaaaa acaatattca tgtgagtaaa ttatagaaaa tttagtacaa
      481 ctattcgcac tcactaaaaa atgaatatta ttcttcgttt atcatttaat tttttaggtt
      541 atataacata tattgtgggt taagttaaag attagtaatt tcataaaaca caccaagtca
      601 gttttttata aatttatttt tttttaagtt tcttaagatg tttaattcat gcggatgaaa
      661 ggtaggcagt ggtagcttta tgaatagcga tatttggtaa aatagggttt atccaagtgt
      721 gagctggggt taataaaata taaattgtgt tagacttatt gattatatat taaataaaaa
      781 tattttatta tttttgtcaa aattttttat tatggaaaaa ttattaaaaa attcaaaaga
      841 atacctcgct ctccctaaag atcacaaacg tgattttctt gatcagcttt atcaatttct
      901 tatagacgac catgctacag tagtagatta tcaaaagcta aaaatccaat ctacagcagc
      961 catttgtcca aactgtaaaa gtgaaaacgt tatcaaagct ggtactcgca aaggtagaca
     1021 gatatataga tgcaaaaatt gtagatatca attcagtgaa acagcacgca cctttgtata
     1081 tcgtatgcgc aaatatccat taatgttaga ctatatacgc tgtatgctcg agggtaaaag
     1141 cttaagagct tgtgctgatg aagtaggtat ttgcttaaaa acaagttttc gatggaggca
     1201 tagaatatta gcagcattga gatcatttga aggaggtatt agtttctctg gcatagtaga
     1261 ggcagatgaa ttgctaatgc aatattcaga aaaaggaagg aaatacaaaa gcagagaaga
     1321 atatgaaaaa gcaaagaaga agcgacatcc caaggtagca gtattagtag ttacagacag
     1381 agaaggtaat ttacttttca aacatgtagg agataaaaaa gttaagaatg agcaattgaa
     1441 aaaagagcta aataaaagga taacagagag taatgtacta tgtgtaaagc ctaaaaagga
     1501 atttaagaag gcagttaaag acataaatag taaaaaagta atagtaaaca aaaagcaatt
     1561 aaagagaggt ttatacagtg tagagatagt agagagcaag atagacaatt ttaagaaatg
     1621 gttgacaaga tttcacgggg tagctactaa gtacttgcag agctatttga tgtggttcgt
     1681 agtgatgaat aagtacctaa aagatattat agagccagac attggtaggt tgctaaactt
     1741 gtcatcgcac gacagatggg cgtgggacaa gtattggaaa ttaatgaaat taaaatataa
     1801 ataatagcaa ttaagtataa cagaatatta attctacaac ccccttccct catgccttca
     1861 aattcaactt attaaaagct gaataattat ctcaataagt attcgctcca atatttgttt
     1921 gcatttctta tgtaaataca attttatcca aaattttatt gtccatacaa aatgttatat
     1981 aacaattttt tacttcttta aaaatttata atacctcatt aaaaatacat attattgatt
     2041 gattaatatc aataactcat tttttataat attggatgtt aatgtatatc cttgattata
     2101 agtgtttctt tagaacattc tcattttaga tgttattttt taataaagaa aaatattctt
     2161 atctttgcaa aaacaaaaaa aatttgatta tggcatacgt aattactgat gattgtacag
     2221 catgtggttc atgcatagat gaatgtcctg cagaggctat ttctccagga gatatttata
     2281 aaatagatcc agaactttgc actgattgcg gttcttgtgc agaagtttgt cctgtagagg
     2341 ctattcatcc tgaataagca attaaagata aagcatttaa aaaaagggag tttaattgct
     2401 ccctttttta ttttaacaaa ttttttaaaa attgtgctga gtaggactta ttaatgcatt
     2461 cacaatcatc tatctctact atttcgtttg attgcaatcc taaaaatatt gctaggagag
     2521 ctacacgatg atcattatga gaatgaattt tgccaccatg catttgcgaa ggataaatta
     2581 tccaattatt gtcatttatt acatctaatt tgccaccaag attaataaaa gtttctgata
     2641 aagtattagc tctattagtt tctttatttg tgaggcggca gataccattt attatagatg
     2701 ggctattagc attagcagct aaaatcatta atgcgggtac taaatctggt gtatctgtaa
     2761 tatcatatgt gtagcctgga tagctctgtc tatgagtaat gatgctttgg tctttttcat
     2821 ttacttgata tcttattgat aaattatcaa aaagttttaa aattttgctg tctggttgtg
     2881 gggaatgtgc agttaaattt ttcacttcgc agctatcaat agttaatcct aaagccaata
     2941 tcatagctcc taatgaccaa tcaccttcta tgaaaaaatc tttatacaat tctattgatt
     3001 tttggggagt aattatgaaa tttttatcat taaattctat aattttacca tacatattta
     3061 tgatatctgc tgtcaaatat acatatggtg cactcactaa tgatgagcaa gatatcgaag
     3121 aaggggcatt attaaagcta cgtgatatta ataatcctga gactgcttga gaacttttat
     3181 cagcttttat gtaataatta tcttgtgagc ttaaattacc aaacacagaa aaaggtaatt
     3241 taaaaatatc acattgcgat atatcactgc tatattgtag tcctaatggt gtgagcatgt
     3301 ctttcagatc tccgaaatct ctatttagta aagttttgtc tcccgtatat ctacctttat
     3361 tttgcaagct atgattgata ggtacaagca ttctcagcaa agtagccgat tccccgcaat
     3421 atatttcttc tggtaattga ttatttatct gaccagaaat tttaatagta ttttcattat
     3481 tgaagttaat agttttgccg aaagatttta aaatattgat tgcagccctc acatcatcac
     3541 acaattgaat atttctcaat atgatttcgt tgctat
//
LOCUS       DAOPRN010000107         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_21700_length_3464_cov_103.896451, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000107 DAOPRN010000000
VERSION     DAOPRN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21700_length_3464_cov_103.896451"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1243
                     /locus_tag="PKI65_08220"
     CDS             107..1243
                     /locus_tag="PKI65_08220"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOB78453.1"
                     /translation="MNNPIFVTKPSLPELDEFIPYLEKIWESKILTNNGPFHQELEKE
                     LSKYLGVPYISLFANGTLALITALQVMRISGEVITTPYSFVATSHSIWWNNIKPVFVD
                     IESDFCNINPEKIEAAITPQTTAILPVHVYGNPCKIEQIQEIADTYGLKIIYDAAHCF
                     GVKYKEQSLCNFGDLSILSFHATKVFNTFEGGAIICHDENTKKRIDYLKNFGFAGETT
                     VVAPGINAKMNEFQAALGLLQLKKLDEYLEKRRIITNTYRELLQDIKGISILPEPPDT
                     KSNYAYFPIFVDEKEYGMSRDELYEKLKTHNIYGRRYFYPLITHFSPYRSLESAKPEN
                     LPVAERIANQVICLPIYSDLDLENVNFIVKIIKYLEDKSKALNH"
     gene            1422..1961
                     /locus_tag="PKI65_08225"
     CDS             1422..1961
                     /locus_tag="PKI65_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOB78454.1"
                     /translation="MSSFYTNEELSNFGLKKFGDKVFISRYAQFYSPETITIGNNVRI
                     DDFCILSGNIIIGSNIHISAYVALYGSKGIILENYTGISPRCTLFSAMDDFSGKYLIG
                     PIHPRELTNVSGGKIILKQYSQIGAHCVIFPNVTIGEGSVVGACSLITKSLDDWGIYA
                     GIPAKWIKPRDTRIKNINY"
     gene            2109..3305
                     /locus_tag="PKI65_08230"
     CDS             2109..3305
                     /locus_tag="PKI65_08230"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: protein motif:HMM:NF019073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0036065 - fucosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HOB78455.1"
                     /translation="MNNLHLMLDSNFTRDFIEVMESMKLFDIHKYLIYNVNNPSELKF
                     VDSNKVIVARYNTKEFNNAIGDIKSYKRIFIHFLSVEMCKFLNKLPALDNIYWIFWGA
                     DFYSPPSCFFDFLYDKHTQEYMKKYMFNRGQAVNIPSSILNNFVIKKILLLRDKKLYN
                     IKAKAISKVSYFLHFNKYDYDIVKSFFKTDMKFLPFRYGHVEFNDSDILFPEIMNDLK
                     NKYSPSSYKIIFVGNSGDPTNNHLDLFYLLKNLNIKEKFKIICPLSYGNKKYIDFIIK
                     KGKEFFGDDFIAITQYLDKDIYNNLIACSDIMIMYHNRTQGVGNINIALHAGKKVFLK
                     KENTTYKLYKDLDLKIFNTEHDLMNNDIFDELTDEDKANNKKIIKDVFSAETQKENLY
                     FILSNH"
BASE COUNT         1283 a          439 c          511 g         1231 t
ORIGIN      
        1 acaactgaca acatacaact aatataggat aaactcttac acttttaacc cttcctcttt
       61 catttgtaga taaaaaataa aaacaaaaaa tattaatcgt aattttatga ataatccaat
      121 ctttgttact aaaccttctc ttccagaatt agatgagttt atcccttacc tagaaaaaat
      181 ttgggagagt aaaatactca caaataatgg tccatttcat caagagcttg agaaagaact
      241 ttcaaaatac ttaggtgtac cctatatctc attatttgct aatggcacac ttgctcttat
      301 aacagcatta caggtaatgc gcatttcagg cgaagtaatt actacaccat atagctttgt
      361 agctacatct catagcatct ggtggaataa tataaaacca gtttttgtag atatagaatc
      421 tgatttttgt aatataaatc cagaaaaaat agaagctgcc attactccac agactactgc
      481 tatattacct gtacatgtat atggtaaccc ttgtaaaatt gagcaaatac aagaaatagc
      541 agatacctat ggtctaaaaa taatctatga tgctgctcat tgttttggtg taaaatataa
      601 agagcaaagc ctttgcaatt tcggtgattt gtctatcctt agttttcatg cgactaaagt
      661 ttttaatact ttcgaaggtg gcgctattat ctgccatgac gaaaatacta aaaaacgcat
      721 agattattta aaaaatttcg gctttgctgg cgaaactact gttgtggccc caggtatcaa
      781 tgctaaaatg aatgaattcc aagctgctct tggtcttttg caacttaaaa aacttgatga
      841 gtatcttgaa aaaagaagaa taattactaa tacatataga gagcttttgc aagatatcaa
      901 aggcatatct atactaccag agccacctga tacaaaatct aattatgcat attttcctat
      961 ctttgtcgat gaaaaagaat atgggatgag tcgcgatgaa ttatatgaaa aattaaaaac
     1021 acacaacatt tatggacgta gatactttta tccgttgatt acccatttct caccatatag
     1081 aagtctagag agtgctaaac cagagaatct gcccgtagct gagagaattg caaatcaggt
     1141 tatctgcctg ccaatatatt ctgatcttga tttggaaaat gtaaatttta ttgttaaaat
     1201 aataaaatat ttggaagata aaagtaaagc tttaaatcat tagtttagat ggtatttatc
     1261 ttagctatca tacactaaat gatgtggttg atgattttca taaaacttga ctaatacata
     1321 taagatagtg tggaggtaat ttgtttactg atctatccta aattgtataa ggtacatatt
     1381 gatattattt gtaatataat agtttaataa attggataaa tatgagttca ttttatacaa
     1441 atgaagaatt atcaaatttt ggattgaaaa aatttggaga taaggttttt attagtcgtt
     1501 atgcacagtt ttattcacct gaaacaatta ctattggtaa taatgtccga atcgatgatt
     1561 tttgtatttt atccggaaat attattatag gttcaaatat tcatatttca gcttatgtag
     1621 cgttatatgg ctctaaagga ataatattag aaaactacac aggtatctcg ccccgttgta
     1681 cactttttag tgcaatggat gactttagtg gaaaatattt gattggtcct attcatccac
     1741 gtgaattaac gaatgtatcg ggaggtaaaa ttatacttaa acaatattct cagattggag
     1801 cacattgcgt aatatttcca aatgttacaa taggagaggg aagtgtcgta ggagcttgct
     1861 ctttgataac aaaatcatta gatgattggg gtatttatgc tggaatacct gctaaatgga
     1921 ttaaaccaag ggatactaga ataaaaaaca taaactattg aaatatgaaa actccttttg
     1981 tttctattcg taattaattg tagatctaaa taaaatatag tctaccgttt tttgtgtctt
     2041 gtaataatta attagttgtt tgtcgagagt tgtttttgtg tcttttgttt tattgaataa
     2101 ttttttttat gaataattta catttaatgc tagatagcaa ttttactaga gattttatag
     2161 aagtaatgga gtctatgaaa ttatttgata ttcacaaata tctcatttat aacgtaaata
     2221 acccttctga attaaagttt gttgatagta ataaagttat tgtagctcga tacaacacaa
     2281 aggaatttaa taatgctatt ggtgatatta aatcttataa aagaatattt attcattttc
     2341 tctcagtaga aatgtgcaaa tttttgaata aattaccagc tctggataat atttattgga
     2401 tattttgggg ggctgatttt tatagtccac caagttgctt ttttgatttt ctctatgaca
     2461 aacatactca agaatatatg aagaaatata tgtttaatag aggccaagca gtgaatatac
     2521 cttcgtctat tctaaataat tttgttatta aaaaaatttt gttattaaga gataaaaaat
     2581 tatataatat taaagctaaa gcaattagta aagtatccta ttttttacat tttaataaat
     2641 atgattatga tattgttaaa tcttttttca aaactgatat gaaattttta ccttttagat
     2701 atggtcatgt agaatttaat gattctgata tattatttcc tgaaataatg aatgatttaa
     2761 aaaataaata tagtccatca tcatataaaa taatctttgt tggcaattca ggagatccca
     2821 caaataatca tttagacctt ttttaccttt taaaaaattt aaatattaaa gaaaagttta
     2881 aaattatttg tccattatct tatggtaata aaaaatatat tgactttatt attaaaaaag
     2941 gaaaagaatt ttttggcgat gacttcatag ctattactca atatttagat aaagatattt
     3001 ataataattt gatagcctgt tctgatataa tgattatgta ccataataga acgcaaggtg
     3061 tagggaatat aaatattgca cttcatgcag ggaaaaaagt ttttttaaaa aaagaaaata
     3121 ctacctacaa attatataaa gaccttgact tgaaaatatt taatactgag catgatttga
     3181 tgaataatga tatatttgac gagttgactg atgaagataa agcaaataat aaaaaaataa
     3241 taaaagatgt tttttcagct gaaacacaaa aagaaaattt atattttatt ttgagtaacc
     3301 actaataaca ttcaacagtc aattgatgtt ttgctaaact tataaaatta tatactataa
     3361 aaaaatcatg taaactcaga acgttacagc tagttatagg ttataagtta aaaatatttc
     3421 cattttgcat taaaaatagt gaaaaaaaga aatgttttat tctt
//
LOCUS       DAOPRN010000108         3373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_22450_length_3373_cov_171.691079, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000108 DAOPRN010000000
VERSION     DAOPRN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22450_length_3373_cov_171.691079"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..773)
                     /locus_tag="PKI65_08235"
     CDS             complement(327..773)
                     /locus_tag="PKI65_08235"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB78456.1"
                     /translation="MNKIRNSIVSYRPWLIVIFTIILISCTNKMDKVRELKLEAINNI
                     YNMQNDDAKKNLEKALKINAKDPEIYYLLGNVYFNEKNYDEAIKYYTEAIEIDSTYAQ
                     AYTSRGKIYRIYNKRDEWCADFTKAYQLGDKTVYNDVRHCIPTIDN"
     gene            complement(766..2109)
                     /locus_tag="PKI65_08240"
     CDS             complement(766..2109)
                     /locus_tag="PKI65_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOB78457.1"
                     /translation="MGAFHAYDIRGIYGVDFNKDDVVKIGYFFPKIFKIKKILIGRDT
                     RTSSDEIHDALIQGLLSSGCDVYDTGLATTPSIYWGTAKYDFDASIMITASHNPKEYN
                     GLKFSGKNATPIGYDDGLNKIEKLIQENEKIEIVPKGTYQEFNFRNEYVDFLKKYLKQ
                     NLQLNFTIDCSNGMAGLYVHEIFTNQAKYMFDDLDGTFPNHEANPLEEKNLKDLKKIV
                     LEQKNDFGMIFDGDADRVMFVDEKGNFISPDLMIGLLGHYFLEERNQKGKVLQDIRTS
                     KSVAEYLEPMGGEIVMWRVGRAYAATKLREIDGIYGGELAGHYYFRDFYYSDSGLLAS
                     ILITNIISDFIKKGYKVSEIIAKIKKYENSGEINFKINHKLEAMNAVKDYFIKEQQPI
                     AFYDFDGYRLDYNDWWLNIRPSNTEPYLRLIVEAKNNELLEEKLKIINNIISNYE"
     gene            2210..3202
                     /locus_tag="PKI65_08245"
     CDS             2210..3202
                     /locus_tag="PKI65_08245"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOB78458.1"
                     /translation="MKHYIGFVLVFFVYFDINSQINFSDLDRLERITKDMKVLSHDTL
                     MGRQSASKYEWKAGNYIINELKKLQSFQNNINFLPNYDRFRFAFTIKNYENNKRDTTA
                     DIIAYIDNKAQYTIIIGAHYDHVGYEIYDSTKGQYLIYNGADDNASGTVGLLELTRHL
                     SNKFLTNYNYIIGFWGSEEIGLYGSAYFCQKILPHINVSPIILYVNYDMIGSLGYKKN
                     ILTIYGTATANDLKDMVPDYYDSVKINKLPMAFPFSDHTYFYANKIPYLYFTTGLPKT
                     YHTIKDEINLINFNGILFTIKLTEHIISQLNPQELQFKKIKLHHYLHSAAASIF"
BASE COUNT         1167 a          522 c          373 g         1311 t
ORIGIN      
        1 aaacaaaata taacctacta tttcccttag tttaaaaaat ttaattttaa attttttaaa
       61 ctatcatttt gctaatacct ctcctaaaaa tcttgaaaaa ttaaccaccc aactactcat
      121 tacgctatat taaaaccaca taaactaaaa attgctttaa aataattaag aattttattg
      181 gaaaggttta atgttttttg aaaaaattac ttaaccctac ttaaaatttt aaaatcttaa
      241 actcatcaac taaaaaaaaa tatttaaaaa atacatataa tcctatatat tcacttaaat
      301 agctaatttt aattctaata atttttttaa ttatctatcg tagggataca atgcctcaca
      361 tcattatata ctgttttatc tcctaattga tatgctttag taaaatctgc acaccactca
      421 tctcttttat tatatattct atatatttta cctcttgatg tatatgcttg tgcgtatgtg
      481 ctatctattt ctattgcctc agtataatat tttatagctt cgtcataatt tttctcatta
      541 aaatacacat tgccaagtaa ataatagatt tcgggatctt ttgcatttat tttaagtgct
      601 ttttctaaat tttttttagc atcatcattt tgcatattat aaatgttatt aatagcttct
      661 agttttagct ctcttacttt atccatttta tttgtgcagc ttattagtat tattgtaaat
      721 atcacaatta accatggtct atagctcaca atactatttc ttattttatt cataatttga
      781 tataatatta tttattattt tcaatttttc ttctaaaagc tcattatttt tggcttctac
      841 tattaatctc aaatatggtt ccgtattgga aggtcttata ttaagccacc aatcattata
      901 atctaatctg tagccatcaa aatcataaaa agctattggt tgttgctctt ttataaaata
      961 atcttttact gcattcatcg cctcgagttt atgattaatt ttaaagttaa tctctccaga
     1021 attttcatat ttttttatct tagcaattat ttcagatact ttgtaaccct tttttataaa
     1081 gtcagaaata atatttgtga ttaatatact agccaatagt ccagagtcgc tataataaaa
     1141 atctctaaaa tagtaatgtc ctgcaagctc tccaccatag atgccatcta tttctctcaa
     1201 cttagtagct gcatatgctc taccaactct ccacattact atttcaccac ccattggttc
     1261 taaatattct gctacagatt tagatgtacg tatatcttgt agtactttac ctttttgatt
     1321 tctttcctct aaaaaataat gtcctaaaag accaatcatc aaatctggtg aaatgaaatt
     1381 acctttttcg tctacaaaca ttactctatc agcatctcca tcaaagatca tcccgaaatc
     1441 atttttttgt tctaaaacaa ttttttttag gtcttttagg ttcttttctt ctaatggatt
     1501 ggcttcatga tttgggaaag taccatctaa atcatcaaac atatatttgg cttgattggt
     1561 gaatatttca tgtacataca agccagccat tccattgcta caatctattg taaaatttaa
     1621 ttgtaaattt tgttttaaat atttttttaa aaaatccaca tattcatttc taaaattgaa
     1681 ttcttgatat gtccctttag gtacaatttc tattttttca ttttcttgaa ttaatttttc
     1741 aattttattt aatccgtcat catagccaat gggagtagca tttttaccag aaaattttaa
     1801 accgttatat tctttgggat tatgactagc tgttatcatt atagaagcat caaaatcgta
     1861 ttttgctgtc ccccagtata tagaaggtgt agtagctaaa cctgtatcat aaacatcgca
     1921 acctgatgac aataagcctt gtattaatgc gtcgtgtatc tcatctgatg atgtcctcgt
     1981 atctctacca attaaaatct ttttaatttt aaaaattttt gggaaaaaat atccgatttt
     2041 aaccacatca tctttattaa aatctacacc gtagatgcct ctgatatcat aagcatgaaa
     2101 agctcccata attttttttt gacaaattta caatattttt aattatatat tttaaaaact
     2161 cttctattat tgtactataa attttttaat ttcgtaaaat tttcatctta tgaaacatta
     2221 tattggcttt gttttggtgt tttttgtcta tttcgacata aactctcaaa tcaatttttc
     2281 agatctagat agattggaaa ggatcaccaa ggatatgaaa gtattatcgc atgatactct
     2341 aatggggagg caatctgcta gcaaatatga atggaaagca ggcaactata taattaatga
     2401 attaaaaaaa ctacaatcat ttcaaaataa tatcaatttt ttgccaaatt atgataggtt
     2461 tagatttgca tttactatta aaaattatga aaataataaa agagacacca cagctgacat
     2521 aattgcatat attgacaata aagctcaata tactataata attggggcac actatgatca
     2581 tgttggttat gaaatttatg atagcactaa aggtcagtat ttgatttata atggtgcaga
     2641 tgataatgct agtggcactg tgggattatt agaactaaca agacatcttt ctaataaatt
     2701 tttaacaaat tataactata tcataggttt ctggggcagt gaagagatag gattatatgg
     2761 ttctgcttat ttttgccaaa agattttacc tcatatcaat gtatctccta taattttata
     2821 tgtaaattat gatatgattg gtagtttagg atataaaaaa aatattctta ctatttatgg
     2881 tacagctact gcaaatgatt taaaggatat ggtacctgat tattatgatt ctgttaaaat
     2941 caacaaatta cctatggctt tcccattcag cgatcataca tatttctatg ctaataaaat
     3001 tccatattta tacttcacta ctgggttacc taaaacttac catactataa aagatgaaat
     3061 aaatttaata aattttaatg ggattctttt tactattaag ctaacagagc atataatctc
     3121 acaattaaat ccacaagaat tgcaattcaa aaaaataaaa ttacatcatt accttcattc
     3181 tgctgcagca tctatatttt aaattttaga gattttttaa atgctttaaa atacatttta
     3241 aaaaattatc aaaaataatg tcaaattgac actattttta tatggcatac tttatgatta
     3301 tgcaaaagca aaacttcaaa aaaaacaaaa gatatgagtg aaattaaaat taaaccttta
     3361 gcagatcgag tag
//
LOCUS       DAOPRN010000109         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_26194_length_3007_cov_114.905488, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000109 DAOPRN010000000
VERSION     DAOPRN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26194_length_3007_cov_114.905488"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            514..1218
                     /locus_tag="PKI65_08250"
     CDS             514..1218
                     /locus_tag="PKI65_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78459.1"
                     /translation="MELFINNKELISKLNQIAMAYVPALELDGEINLLSNTYYSTIMS
                     ILHFGEGELNFRKGERFNELNLSPQFVRYCNDQLKEWNERGINSLDIAINTIEEFIKS
                     GILSKDPVSEKNFMDMRIRIAEVLAESPLKVTDVSKLMSAFDDVVSQAARQGELGVIT
                     FMRDKLVELKKVRLSPTRGTEDNIAVWKLIAALIIFGFPVYKSLRCILKKKCCNTVSG
                     LEGAIVFIAAFAWTLC"
     gene            1239..1577
                     /locus_tag="PKI65_08255"
     CDS             1239..1577
                     /locus_tag="PKI65_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78460.1"
                     /translation="MKTKVILIILSALLTLSACTSTQYVALSSTDKKISNIEVFLRDV
                     QKPVKEYEIISYIETSGTIFTTKKQLLIGLKKKVDELGGDAVIDVHFFYIPWFLSSLP
                     AVDGAVIKYK"
     gene            2157..2966
                     /locus_tag="PKI65_08260"
     CDS             2157..2966
                     /locus_tag="PKI65_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78461.1"
                     /translation="MEPNFFEIAKSQFLQNHFFVWLLNWANDENEDFNKELNEIAKNF
                     VRLLLGEKPDFPIKNVKAVGQWNNIDIKVAVNDEYIIAINNKLSDTCFTTQLMDYKIQ
                     INEHYRTKKNKFIYLITKNDDWYYYSGIRDLSFDIVDRQMILDFMNKQQTDNDIFNDY
                     KDYLNKIEIETNSFNKYHNIITNSYAAEGFYLKLKNIIMEKNLLAMWEYDSDMNGDFL
                     KFYYYDLYKIENIGMLFIQLENNINGSDIQLNIKICQWEPSTDNLQKILVL"
BASE COUNT         1162 a          373 c          455 g         1017 t
ORIGIN      
        1 tttatccaaa tttactattt ttttatttac ccactcaaaa cattatagaa cctaattgtt
       61 ctattaatta ataggacagt taaatattaa ccacaaagtt ctcaaagaat gcacaaagaa
      121 cgtagagaag gatggaaaca catatataac aagtagtacc ttgtaaattt tgtgtaaagt
      181 attgtgtcct ttgtggtaaa aaatattatt aataattaaa ataatttggt tgaatgaaaa
      241 aaaattataa ctttgtatgt gtaaatttat ttacgtagtt acaaaatgaa ctatttgaga
      301 catatattat tgcttacatt ttcaacattt tatcaagata tctctactgt ggataatgaa
      361 aatataaatc taatggtagg tttacacaga tgttagcgtt catgcttaaa aaaacaactt
      421 ataatgaaat ggaagaaaaa tttattatat ctcaaaataa aggaacaata atctcgttaa
      481 taaagaatat gttaaactaa aaattaaaaa attatggaac tatttataaa caacaaagag
      541 ttaataagta aattaaatca aattgcaatg gcttatgtac cagctcttga acttgacgga
      601 gaaattaatc tgttaagtaa cacttattat tcgaccatca tgtcaatatt acattttggc
      661 gaaggagaac ttaattttag aaaaggtgaa agatttaatg aattaaacct ttcaccgcaa
      721 tttgtcagat actgtaacga ccaattaaaa gaatggaatg aaagaggaat aaattctctt
      781 gatattgcga ttaatacaat tgaggaattt attaaatctg gaattttaag taaagaccca
      841 gtatctgaga aaaatttcat ggatatgcgt atacgaattg ctgaagtttt ggcagaatca
      901 ccactaaaag ttactgatgt atcaaaatta atgagtgctt ttgatgatgt tgtgtcacaa
      961 gcagctagac agggtgaatt aggagtaata acttttatgc gtgataaact tgtcgaatta
     1021 aaaaaagttc gtttatctcc tacaagaggt actgaggaca atattgctgt atggaaatta
     1081 attgctgctt taattatatt tggatttccg gtatataagt ctttaaggtg tatattaaaa
     1141 aagaaatgtt gtaatacagt tagtggactt gaaggtgcca ttgtatttat tgcagcattt
     1201 gcctggacct tatgttaatt catataattt atagtattat gaaaacaaaa gttattttaa
     1261 ttatcctaag tgctttattg acattgtctg cttgtacttc aactcagtat gtagcactta
     1321 gttctactga taaaaaaatt tctaacattg aagttttttt gcgtgatgta caaaaaccag
     1381 taaaagaata tgaaattatt tcatatatcg aaacatcagg gacgatattc actacaaaaa
     1441 aacaactttt gataggtcta aaaaagaaag ttgatgaatt aggtggtgat gccgttatag
     1501 atgtccattt cttttacata ccatggtttc tttccagttt accagcagta gacggtgcag
     1561 taataaagta taaatagagt attaatcttt attttgagtt tataaattag aacgaaagca
     1621 cgaaacgcta acaggcggta tagttaattg cctgtgcagt gcgggtttca gcatataata
     1681 aacttttgtg taacttgaca ggtcagtact tcgaaatatc cgccatctct tatcttagca
     1741 gttaccgtct cacaaataat ttttcgtttt caaataaatt attctacgga ctttacccag
     1801 accacaagcg accaattata agtttaaatt tttattcact atttggttat tttttctatt
     1861 acgtagtcgt ctattaaaaa gtatttatcc tattaaatat gaagaatatt tttggaaaaa
     1921 ttttttttga taatcaatat taaacaaaag tttatattca agcatagctg aaaagatgat
     1981 ttaattcgtc gctgccacaa aaaaatttat aacgacacgt tttgtccacc atacaccata
     2041 tttttgctaa tgaaataaaa aacgaaaaga taaatattag aatgagccac atgcacctgt
     2101 tcaatattat tttttaaaat ttgaaattaa caagattaat ccaaaaatta aatattatgg
     2161 aaccaaactt ttttgaaatt gcaaaatcac agttcttaca aaatcatttt tttgtatggc
     2221 tactaaattg ggcaaatgat gaaaacgaag attttaacaa agaattgaat gaaattgcaa
     2281 aaaattttgt gagattattg cttggtgaga aacctgattt ccctataaaa aatgtaaaag
     2341 ctgttggaca atggaataat atagatatta aagtcgcagt aaatgatgag tatatcatag
     2401 ccattaataa taaactatca gatacttgct tcacaacaca attgatggat tacaagatac
     2461 aaataaatga acattaccgc acgaaaaaaa acaaatttat ctatttgata actaaaaatg
     2521 atgattggta ttattattcg ggtataagag atctatcgtt tgatattgta gatagacaaa
     2581 tgattttaga ttttatgaat aaacagcaaa ctgataatga catctttaat gattacaaag
     2641 actacttgaa taagatagaa attgagacaa attcatttaa taaatatcac aatattatca
     2701 caaatagtta tgcagcagag ggtttttatc taaaactaaa aaatataata atggagaaaa
     2761 acttacttgc tatgtgggaa tatgattcag atatgaatgg tgattttctt aaattttact
     2821 attatgattt atataaaatt gaaaatattg gtatgctatt tatccaatta gaaaacaata
     2881 taaatgggag tgatattcaa ttaaatatca aaatttgtca atgggaacca agtactgaca
     2941 atctacaaaa aattttggtt ctataacgga tagtgtgggt aatcaaattt tagcttacca
     3001 cataaga
//
LOCUS       DAOPRN010000110         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_26667_length_2965_cov_85.269759, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000110 DAOPRN010000000
VERSION     DAOPRN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26667_length_2965_cov_85.269759"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..294
                     /locus_tag="PKI65_08265"
     CDS             40..294
                     /locus_tag="PKI65_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78462.1"
                     /translation="MSYYDIVKPNFRIEYWLNCCRGLVGQNREDWINLIILPTNTNSE
                     LADEHKQYAAIIDLINTRCFDENNLLESIVEQILDELLNN"
     gene            complement(291..1250)
                     /locus_tag="PKI65_08270"
     CDS             complement(291..1250)
                     /locus_tag="PKI65_08270"
                     /inference="COORDINATES: protein motif:HMM:NF021631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOB78463.1"
                     /translation="MDVLFKTIFGAHLYGTETADSDIDYKAVYAPTLEDLILGRVKLD
                     SSIRESNKKDIHIKSDANVYEIEYYPLQKFLKHAAEGQLAAIDMLYSPPQFWIGKVSD
                     FFIAEVINKREKFLSKNLIKFVQYAVKQAAKYGIKGSRLHTATKFLKFFENIDEKIRL
                     NDIIIDIEPLLDDHSEIYYIDNNPAESMLIICGKKFSFSTKVRYIRNALRKFVDKYGA
                     RAKLAVIDKGIDKKACSHAFRAAFEVKELVLTRNLVFPLKEREFLRDIKTGKYTYSEF
                     APMLEDLIDEVSHLVIKSDLPDTPQIDVNEIIKKYYAVKSAIF"
     gene            complement(1276..1524)
                     /locus_tag="PKI65_08275"
     CDS             complement(1276..1524)
                     /locus_tag="PKI65_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78464.1"
                     /translation="MVDISVDELKKIMLKQNFLLDKLMNACINSDAAANSYSEYACRE
                     FLKNIYMTWCEVDVGYKSLLKIIKDLEYEYITEKTEKR"
     gene            complement(1533..1808)
                     /locus_tag="PKI65_08280"
     CDS             complement(1533..1808)
                     /locus_tag="PKI65_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78465.1"
                     /translation="MGKLIGFKYEPTDLCTGTFEIYEEDKSIIARIRYDKNGKSLSII
                     DISIIPDNEKKLFFKESISDIISDDYLRKYFIYSIYDVAGIPTEEEI"
     gene            complement(1867..2436)
                     /locus_tag="PKI65_08285"
     CDS             complement(1867..2436)
                     /locus_tag="PKI65_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOB78466.1"
                     /translation="MSKINDTITKAIEEHEKWLSSWGDNGKRIKIQNITLQGSNIFCN
                     RDLRYASFMNVAFIECDFTNANLVGAKMQGNFSRSIFKNADLSYINAFGANFEGACLN
                     NTSLHKAILNKTNLCGVDMRGSTICKALLSEADLRNAEFDVYVLFEIMKLYRSVIAAE
                     FDGNIEARHDMPILDDYVYMLIRGFTFGS"
     gene            complement(2485..2850)
                     /locus_tag="PKI65_08290"
     CDS             complement(2485..2850)
                     /locus_tag="PKI65_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78467.1"
                     /translation="MGRIKVVSERIFVNGEPLYKIQHVEAAPMNKLPDSYLNGYPYCY
                     YVRTQHSEYLEVSWIDNDKVVTHYIVVGEVYTVQNFDMIIEHVREAGERLSNMFKGWR
                     EMCFKDGVNIWKGTRVDII"
BASE COUNT          977 a          522 c          330 g         1136 t
ORIGIN      
        1 tgttagaaat aatttatgtg tagcaggtga ttacaaatta tgagctacta tgacattgta
       61 aaacctaatt ttagaattga atattggtta aactgttgtc gtggtttagt tggtcaaaat
      121 agagaagact ggattaatct tattatatta ccaactaata caaattcaga acttgcagat
      181 gaacataaac aatatgctgc gattattgat ctaattaaca cacgttgttt tgatgaaaat
      241 aatttattag aaagtatagt agaacaaata ttggacgaat tactcaacaa ttaaaatatt
      301 gcacttttta ctgcataata ttttttaatg atctcattta catctatttg aggagtatct
      361 ggtaaatcag atttaataac tagatgacta acttcgtcta ttaaatcttc tagcattgga
      421 gcaaattcac tataagtata cttaccagtt tttatatctc ttaaaaattc cctttctttc
      481 aacggaaaaa ctaaatttct tgtcaatacc aactctttta cttcaaaagc tgctctaaaa
      541 gcatgactgc aagctttttt atctattcct ttatcgatta ccgctaattt agcgcgagca
      601 ccatatttat ctacaaattt tctaagagca tttcgtatat aacgaacttt tgttgaaaat
      661 gaaaattttt taccacaaat tattaacatc gattcagctg gattgttatc aatataatat
      721 atttcagaat gatcatctaa taatggttct atatcaataa taatgtcatt aagtctaatt
      781 ttttcatcaa tattttcaaa aaattttaaa aatttagtcg cagtatgcaa tcgactacct
      841 ttaataccat attttgccgc ttgtttaact gcatattgta caaatttaat cagattttta
      901 cttaaaaatt tctctctttt attgattact tcagctataa aaaaatcact aactttacct
      961 atccaaaact gtggtggaga ataaagcata tctattgcag ctaattgtcc ttcagcagca
     1021 tgtttcagga acttttgtaa tggataatat tcaatttcat atacattggc atcagattta
     1081 atgtggatat cttttttgtt agattctcga atactagaat ctagctttac tctaccaaga
     1141 attaagtctt ctaaagtagg agcatagaca gctttgtagt caatatcact atctgctgtc
     1201 tctgtaccgt atagatgggc tccaaaaatt gttttaaaaa gtacatccac actaatcttc
     1261 tccttattaa ttattttacc ttttttctgt tttttcagtt atatattcat attctagatc
     1321 tttaattatt tttaataatg atttgtaccc aacgtccact tcacaccaag tcatatatat
     1381 attttttaaa aattctctgc atgcatattc tgaataacta tttgcagcgg cgtcggaatt
     1441 aatacaagca ttcatcaatt tatctaacag aaaattttgt ttcaacatta tctttttgag
     1501 ttcatctaca cttatatcaa ccatttttac ttttatatct cctcttctgt aggaattcca
     1561 gcaacgtcgt atattgaata aataaaatat tttcttaaat aatcatcaga tataatatca
     1621 gaaattgatt ctttaaagaa aagttttttt tcattatcag gaattattga tatgtctata
     1681 atgctcaacg attttccatt tttatcatat ctaattcgtg ctataataga cttatcttct
     1741 tcgtaaattt caaaggtgcc agtacaaaga tcagttggtt catatttaaa accaattaat
     1801 tttcccaata ttagcactct ctttttatat atatttttta tattaatcta tttttaatcc
     1861 cctttcttag ctaccaaaag taaaaccgcg tataagcata taaacataat catctaatat
     1921 gggcatgtca tgacgagctt caatgttacc gtcaaattca gcagctataa cactccgata
     1981 aagcttcatt atttcaaata aaacatatac gtcgaactca gcatttctta agtcagcttc
     2041 agaaagcaac gctttgcata tagtactacc tctcatatca acaccacata ggttagtttt
     2101 attcaatatt gctttatgca aactagtatt atttaaacat gcaccttcaa aattagctcc
     2161 aaatgcatta atataactta aatcagcatt tttgaaaatg cttctactaa aattaccttg
     2221 catttttgca ccaactaaat tagcattagt gaaatcacat tcaataaatg caacattcat
     2281 aaagctagca taacgcaaat ctctattgca aaatatatta ctaccttgca gtgttatatt
     2341 ttgtatctta attcgttttc cgttatctcc ccaactactt aaccattttt catgttcttc
     2401 aatagcttta gtaattgtat catttatttt tgacatttta tatttttctc ctttaatcta
     2461 aataacaccg ttctttttta tttattatat aatgtcgact ctagttcctt tccatatatt
     2521 aacaccatct ttgaaacaca tttctctcca acctttgaac atattggaga gacgttcacc
     2581 agcttctctc acgtgctcta taatcatatc aaaattctgt actgtataca cttctcctac
     2641 tacaatatag tgggtaacta ctttatcatt atcaatccac gaaacttcta aatattctga
     2701 gtgttgtgta cgtacgtagt aacagtatgg atatccattc agatatgaat caggaagttt
     2761 attcatagga gctgcttcaa catgttgtat cttataaagc ggttcaccat taacaaagat
     2821 cctttcactg accaccttaa tcctacccaa tggagcaaga ttcatttctt ctttagacat
     2881 ttctttctcc tccttttcta tattttctat ttatattatt aattttattg tacaataaaa
     2941 ttaatttcat caaattcatc atata
//
LOCUS       DAOPRN010000111         2900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_27479_length_2900_cov_228.015817, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000111 DAOPRN010000000
VERSION     DAOPRN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27479_length_2900_cov_228.015817"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1016
                     /locus_tag="PKI65_08295"
     CDS             24..1016
                     /locus_tag="PKI65_08295"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78468.1"
                     /translation="MEKLLKNSKEYLALPKDHKRDFLDQLYQFLIDDHATVVDYQKLK
                     IKSTAAICPNCRSENVIKAGTRKGRQIYRCKSCRYQFSETARTFVYRMRKYPLMLDYL
                     RCMLEGKSLRACADEVGICLKTSFRWRHRILAALRSFEGGISFSGIVEADELLMQYSE
                     KGRKYKSREEYEKAKKKRHPKVAVLVVTDREGNLLFKHVGDKKVKNEQLKKELNKRIT
                     ESNVLCVKPKKEFKKAVKDINSKKVIVNKKQLKRGLYSVEIVESKIKNFKKWLIRFHG
                     VATKYLQSYLMWFVVMNKYLKDMIDPDIGRLLNLSSHDRWAWDKYWKLMKLKYQ"
     gene            complement(1323..2462)
                     /gene="dnaN"
                     /locus_tag="PKI65_08300"
     CDS             complement(1323..2462)
                     /gene="dnaN"
                     /locus_tag="PKI65_08300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOB78469.1"
                     /translation="MKFIVSNAELSKAINSVSSVPIANNVVKILENFLFSVSNNNLTI
                     TATDGDTRASITINLIESDGEGELVIPSKLLQEYLKNVPSIPIAFNLDTTGENNYQIT
                     ISAGNGVYRMVGMDAGNYPTMPQIPDATSIVLNSSILSTSIEYTLFCAAPVDYRQILT
                     GINFDFKDNMLNVVSTDSHRLVRYRRFNIDTDDNFNFVSKRKPLQLLRNLLDKDTDVT
                     INFNETNVSFEFENIIISCKLLEGVFPDYDRVIPPSFENKLLIGKEDFIAALKRVSVF
                     TDKATNQVKMHIEGQEITLTGNDEEYANEGIEKLQCTYEGEDMEISFNSKTFMEMLQH
                     LDCDNINILMNKPNTAIVITPAEENENEDIVMLHMPISLKGSGDY"
BASE COUNT         1073 a          385 c          438 g         1004 t
ORIGIN      
        1 tatttttgtc aaaatttttt attatggaaa aattactaaa aaattcaaaa gaatatctcg
       61 ctctacctaa agaccataaa cgtgatttcc tcgatcagct ttatcaattt ctaattgacg
      121 accatgctac agtagttgat tatcaaaagc taaaaattaa atctacagcg gccatttgtc
      181 caaactgtag aagtgaaaat gttatcaaag ctggtactcg caaaggcaga cagatatata
      241 gatgcaaaag ttgtagatac caattcagcg aaacagcacg cacctttgta tatcgcatgc
      301 gaaaatatcc cttgatgtta gattatttac gctgtatgct cgagggtaaa agcttaagag
      361 cttgtgctga tgaagtaggt atttgcttaa aaacaagttt tcgatggagg catagaatat
      421 tagcagcatt gagatcattt gaaggaggta ttagtttctc tggcatagta gaggcagatg
      481 aattgctaat gcaatattca gaaaaaggaa ggaaatacaa aagcagagaa gaatatgaaa
      541 aagcaaagaa gaagcgacat cccaaggtag cagtattagt agttacagac agagaaggta
      601 atttactttt caaacatgta ggagataaaa aagttaagaa tgagcaattg aaaaaagagc
      661 taaataaaag gataacagag agtaatgtac tatgtgtaaa gcctaaaaag gaatttaaga
      721 aggcagttaa agacataaat agtaaaaaag taatagtaaa caaaaagcaa ttaaagagag
      781 gtttatacag tgtagagata gtggagagta agataaaaaa ttttaagaaa tggttgataa
      841 gatttcacgg agtggctacg aagtacttac agagctattt gatgtggttc gtagtgatga
      901 ataagtacct aaaagatatg atagaccctg acattggtag gttgctaaac ttatcatcgc
      961 acgatagatg ggcgtgggac aagtattgga aattgatgaa attaaaatat caataatagc
     1021 aattaagtct aacagaatat taatttcaca aatccttctc ctacttttct caaacgcatt
     1081 tttataaaag cttattagga aacaatttat ttgatacatt tatgttataa tgtatccact
     1141 ttatatttta ttcactcaat ttttattgtc tatacaaaac atcataaaac caatttttag
     1201 aatttaaaat agtttttagt ttttattatt tattggtagt tttattaaag aaaatagggg
     1261 aacaaaaaat ttttgttccc ctattttaaa aaaaattaat atttatgaag caaatttagt
     1321 ttttaataat caccgctccc tttaagagat ataggcatat gaagcattac aatgtcttca
     1381 ttttcatttt cttctgccgg tgttattact atagctgtat taggtttatt catcaaaata
     1441 tttatattat cgcagtctag atgttgcagc atttccatga aagttttact attaaaagat
     1501 atttccatat cttctccttc gtatgtacat tgtaattttt ctataccttc attagcatat
     1561 tcttcgtcat taccagtgag agtgatttct tgcccttcaa tatgcatttt tacttgatta
     1621 gtagctttat cagtgaagac actaacacgt tttaatgcag caataaaatc ttctttacca
     1681 attaaaagct tattttcgaa actaggtggt atcactctat cataatctgg aaatacacct
     1741 tcgagtagtt tgcaagaaat aataatattc tcaaactcaa aagatacatt ggtttcatta
     1801 aaattaatag ttacatctgt gtctttgtct aataaatttc taagcaattg cagtggtttt
     1861 cttttagaaa caaaattaaa attatcatca gtatctatat taaatctcct ataacgaact
     1921 aatctatgac tatcagtaga aactacattt agcatattat ctttaaaatc aaaattgata
     1981 ccagtaagta tttgtctgta atctacagga gcagcacaaa aaagagtgta ttcaatagat
     2041 gtagataaaa tactactatt tagcactatg cttgtagcat caggtatttg aggcattgta
     2101 ggataattac ctgcatccat tcctaccata cgatatacac cattacctgc agagatggta
     2161 atttgataat tgttttcacc tgtagtatct aaattaaatg ctataggaat cgatggtaca
     2221 tttttcaaat attcttgtaa taattttgat ggtattacta gctctccttc gccatcactt
     2281 tcaataagat ttatagtaat gctggctctc gtatcaccgt ctgttgccgt tattgttaga
     2341 ttattattac ttacagaaaa tagaaaattt tctaaaattt tcactacatt attagcaatg
     2401 ggaacactgc taacactatt aattgcttta cttaattcag catttgagac aataaatttc
     2461 atattttaaa attttgactt caaaattaca ttaaaaattt atattggtaa aaaaatattt
     2521 aataaaatat tctcaatatt tcgtaaattg tagtttaatg ataatttgta attgtagcaa
     2581 agtaatgtct ttttttaata tgatataatt aaacaagaac aaagaattta atttataaaa
     2641 ttagtaaata tccttaataa atttgatatt attaaaagga aattaaaaat taaaagattt
     2701 tttgttaagt tacataatct aattactttc gcataaattt aatttttaaa acgaaaaatt
     2761 tttgaaatat gaaaagaatt tttgtactat tttttttaat attattattt ttcactgcta
     2821 aaagtcaaaa ttcagataga tggagctttg gttcagattt aaaaagaacc tttaatctag
     2881 gtggagaatt gggtctgaca
//
LOCUS       DAOPRN010000112         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_27868_length_2869_cov_105.485785, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000112 DAOPRN010000000
VERSION     DAOPRN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27868_length_2869_cov_105.485785"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..797
                     /locus_tag="PKI65_08305"
     CDS             222..797
                     /locus_tag="PKI65_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOB78470.1"
                     /translation="MNLIELIKSRRTIRKFKQISINIDILIDIIDCARLAPCASNKQP
                     LEYIIVTNKNLCDEIFVNLRWAAYIHPNGIPKHDEKPTCYIAVIVNKNRATKWIGHDI
                     GLAIENITLAAWSYGIASCILGSVNTDNVKNILHIPDEYILDSIVALGYSAQQSFVED
                     NDDTVEYYLDEDGNMYVPKRPLNNIIHFNDF"
     gene            1027..>2869
                     /locus_tag="PKI65_08310"
     CDS             1027..>2869
                     /locus_tag="PKI65_08310"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOB78471.1"
                     /translation="MKKLIILISVLSILISKNLLADEGMWLPVLLEAQYDDMVTRGLQ
                     LSPEDIYSINNSSLKDAIVLFGRGCTGVIVSEQGLILTNHHCGYGSIANVSTVNNDYL
                     TNGFWSNDFKEEIPIEGLTISILVSMRDVTNEILSQIPTNATEKQRKDIANKLCKNIA
                     AENKTKPHIDAVVREFNYGNAYYLMIYEVFSDIRLVAAPPLFIGNFGGDTDNWVWPRH
                     TGDFSVFRIYVNQNNESTNYSKNNVPFKPKYVAPVSKKGINKDDFIFIYGFPGSTTHF
                     MTSKGIDLNVNLINPIAIDIRTTKLNIIENYSRNNDTIRLMYANKRAGIANYWKKMQG
                     ETIGINATKAIEKRHSQEQDFVNWLKSIDSLKKYANLMDTLYHYHQLAYPYEYIDRVL
                     YEAYFSNEIIRFVQRTIYDIQRGIDDSWANKWCYLVNNFLKNNNLKLEQDLFLSTFKY
                     LDSISKYLPNDLLKNKLNTEKNIEKSLFINFNELSVACLKNKINPKTIQKDPLYVFTS
                     KLLDWYVKEILPMEDYYNNKLDSLYRMYTKLQSYYLPQNKYYFDANSTLRIAYGNVKP
                     YHPADAITYIHYTTIDGMVDKALWGQAEDYKIDSTLLELFKNKDFGSY"
BASE COUNT         1086 a          379 c          413 g          991 t
ORIGIN      
        1 aatataacat atgttgattg aaacatatat tagaatccat tcattcaaca ccaaatcttt
       61 cgactaaaaa ttaaaataga agccaagata ttagttattt aatttcactt taaattaaac
      121 tctttaattt ttcttatttt tgctggttca aaaaataaat catctttcaa aaaataaatt
      181 caataatttt tttatttttg cataaattat gatataaaaa aatgaattta atagaattaa
      241 taaaatctcg cagaactata cgaaaattta aacaaatatc cataaatatt gatattttaa
      301 tagatattat agattgtgcc agattagctc cttgtgctag caacaaacaa ccactagaat
      361 acattatagt aactaataaa aatctatgtg atgagatatt cgtcaatctc agatgggcag
      421 catatattca ccctaacggc atacccaaac atgatgaaaa acctacatgt tacattgcag
      481 taatagttaa taaaaataga gctactaaat ggattggaca cgatattgga ttagcaatag
      541 aaaatatcac cctagctgct tggtcctatg gtattgctag ctgtatttta ggttctgtta
      601 atacagataa tgttaaaaat attttacata ttcctgatga atatatatta gatagtattg
      661 ttgctctagg ctactcggca caacaatcat ttgtagaaga taatgacgac actgtagagt
      721 attatctgga tgaagatgga aatatgtatg tgcctaaaag acctctaaac aatattattc
      781 acttcaatga tttttaattt tttttcgaaa attatttttt ttaaaatcca ataagtcaac
      841 ttctaaactc tttacacagt tacatagtat gataaatatt ttcgcatatt aattttatta
      901 tttcacagaa taatctgatt acgatcatta aaaatcaaaa atgaattaat ttgagcgatt
      961 attttcatat tgttattatc ttttattatt tttgctcata aattaactaa aaaaaataat
     1021 tttgttatga aaaaattaat aattttaatt agtgttttaa gtattctaat aagtaaaaac
     1081 cttttggctg atgaagggat gtggttgcca gtgcttttag aagcacaata tgatgatatg
     1141 gttacacgag gcttgcaatt atctccagaa gatatatata gtataaataa ttccagtcta
     1201 aaagatgcta tagtactatt tggtagaggt tgtacaggtg tgatagtgag tgagcaagga
     1261 ctgatattaa ctaatcatca ttgtggctat ggatctattg ccaatgtcag tacagttaat
     1321 aatgattatt tgactaatgg attctggtct aatgatttca aagaggaaat acctattgaa
     1381 gggcttacta tatctatatt agtaagcatg cgggatgtaa caaatgaaat tttgtctcaa
     1441 ataccaacta atgctaccga aaaacaaaga aaagatattg caaataaatt atgtaaaaat
     1501 attgctgctg aaaataaaac taaacctcat atagatgcag tagtaagaga gtttaactat
     1561 ggcaatgctt attatctgat gatatatgaa gtgtttagtg atatacgttt agtagcagct
     1621 cctccattat tcattggtaa tttcggaggc gatacagata attgggtttg gcctaggcat
     1681 acaggtgatt tttctgtttt ccgaatttat gttaatcaaa ataatgaatc tactaactat
     1741 tctaagaata atgttccttt taagcctaaa tatgttgctc cagtgtctaa aaaaggtatt
     1801 aataaagacg attttatatt tatttatggg ttccctggct ccacaactca cttcatgact
     1861 tctaaaggca tagatctcaa tgtaaatctc ataaatccta tagccattga tattagaaca
     1921 acaaaattaa atataattga aaattattct cgtaataatg atactatcag actaatgtat
     1981 gctaataaac gagcaggcat agctaattac tggaaaaaaa tgcaaggtga aactattggt
     2041 atcaatgcta ctaaagcaat agaaaaaaga catagtcaag aacaagattt cgtaaattgg
     2101 ttaaaaagta ttgactcact aaaaaaatat gctaatctaa tggatacttt atatcattat
     2161 catcagttag cttatcctta tgaatacatt gatagggtac tctatgaagc atatttttct
     2221 aatgaaatta ttagatttgt tcaacgtact atttatgaca ttcagcgagg cattgacgac
     2281 tcatgggcta ataaatggtg ttatttagta aacaattttt tgaaaaataa taatctaaaa
     2341 ttagagcaag atttatttct ttcaactttt aaatatttag atagcatatc aaaatatttg
     2401 cctaatgatt tattaaaaaa taaattaaat actgaaaaaa atatagagaa atctttattt
     2461 ataaatttta atgaattatc tgttgcctgc ttaaaaaata agattaatcc aaaaacaata
     2521 caaaaagatc ccctttatgt ttttacaagt aaattgttag actggtatgt taaagaaatt
     2581 ttacccatgg aagattatta caataacaaa ttagatagtc tctatcgtat gtatactaaa
     2641 ctgcagagct attatttacc tcaaaataaa tattacttcg atgctaatag tactcttaga
     2701 attgcctatg gcaatgtgaa gccatatcac cctgcagatg caatcacata tatacattat
     2761 actactattg atggtatggt agataaggca ttatgggggc aagccgaaga ttataaaatt
     2821 gatagtacat tgctagaact ttttaaaaat aaagatttcg gttcatatg
//
LOCUS       DAOPRN010000113         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_28318_length_2831_cov_84.027017, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000113 DAOPRN010000000
VERSION     DAOPRN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28318_length_2831_cov_84.027017"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PKI65_08315"
     CDS             <1..620
                     /locus_tag="PKI65_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78472.1"
                     /translation="DTLTTITDSLKGREFVFNKNVRIFQNDLQAVCDSLVYSQLDSTF
                     TLFKFPIFWFDSTQMTSDTMVLFLKNENLDKMYGFENAFIIEPFMDTLFNQIKGITVT
                     LIFKENEIDSLIMYRQSELVYYLVDDEQKIIGVNHSTGNQTILTFVDRELDKVLILEN
                     PQGTVYPLDEFPKELEKLKGFQTYYYKLIANRDEIYKQLNFNPIE"
     gene            645..1088
                     /locus_tag="PKI65_08320"
     CDS             645..1088
                     /locus_tag="PKI65_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78473.1"
                     /translation="MMKNVIKLLLILLGTIVVAFLIISLIFLMITGKSNAFSFDEKNQ
                     LLKSKNNLNTIQIIGLDAECSNDCYFLKNNSNQQNKKLIKLNNITDNFIITKCDNDSQ
                     IHINDLELKPNCNYKIINTSNPNAEGATIIIYKDKNGIYHQKQAR"
     gene            1242..2246
                     /locus_tag="PKI65_08325"
     CDS             1242..2246
                     /locus_tag="PKI65_08325"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78474.1"
                     /translation="MKNIVSNLQEHLSQGKQLNLEFLNNLYNKMKLQGNDVVDYQHLK
                     AQSVSPQCPNCKSIHIKKNGHQQGQQMYKCKNCGKNFRETTGTFVYYLHKKELMLDYI
                     RLMLEGKTLRQCSKELRISLPTSFEWRHRIISALRSFENNVNFFGIMEIDELQLKYSE
                     KGRRYKTLEEYLLAKELKTHNVAVLSMIDRSGNMLCKLTGLDNVKRDQIDKILLSRIS
                     NNAVICSPDNEEFKNLKTQSIKEKTIITHRTKKYGQHGLAIVKDKENDFAGWINYKFR
                     GVATKYLQSYIMWYVVKHKYLLSRVMDDIGRMLNLAASDWEAWYVYMRLRVKHREKLT
                     "
     gene            complement(2317..>2831)
                     /locus_tag="PKI65_08330"
     CDS             complement(2317..>2831)
                     /locus_tag="PKI65_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78475.1"
                     /translation="IIGLYYFRNAEIIKKSLLKLLENDKKVNGEYQLTDALQYMIEND
                     YKITIQNVDEWLDCGNKNATLLAHKRILERYPSESVVADNVKCKDGIIIPPCYIEGDV
                     LIENSIVGPYVSIGNNSVISHSIITNSILQEHVKLSNVNIDNSMIGKYADYNDSYDEL
                     NIGDYSEISR"
BASE COUNT         1081 a          388 c          421 g          941 t
ORIGIN      
        1 gtgatacatt aactaccatt acagattcat tgaaaggtag agagtttgtt tttaataaaa
       61 atgttcgcat ttttcaaaat gatttgcagg cagtatgcga ttctttagtt tatagtcaat
      121 tagattctac ctttacatta tttaaatttc ccattttttg gttcgatagt actcagatga
      181 cctcagatac aatggtttta tttttgaaaa acgaaaattt agataaaatg tatggtttcg
      241 aaaatgcttt tatcatagaa ccatttatgg atactttatt taaccaaatt aaaggaatta
      301 cagttactct tatttttaaa gaaaatgaaa tagattctct cattatgtat agacagagtg
      361 aattagtata ttatttggta gatgatgaac aaaaaataat aggtgtcaat cactctacgg
      421 gcaatcaaac tattttaact tttgtagata gagagctaga taaggtcttg atattagaaa
      481 atccacaagg aacagtctat cctttagatg aatttcctaa agaattagaa aaattaaaag
      541 gatttcaaac ttattactat aaattaattg caaatagaga cgaaatatac aaacaactca
      601 attttaatcc tattgagtga tttcaattat tctataatta atgcatgatg aaaaatgtaa
      661 tcaaattatt attaattctg ctaggaacca tagtagtagc ttttttgata atctcattaa
      721 tatttctaat gattaccggc aaatctaatg ccttctcatt tgatgaaaaa aatcaattat
      781 taaaatcaaa aaacaatctt aatactattc aaataatagg attagatgct gagtgctcaa
      841 atgattgtta ttttttaaaa aataattcta atcaacaaaa taaaaaattg attaaactaa
      901 ataacatcac agataatttt attattacca aatgcgacaa tgatagccaa atacacataa
      961 atgatctaga actaaaacca aactgtaatt acaagattat aaatacaagt aatccaaatg
     1021 cagagggagc aactattata atttataagg ataaaaatgg gatatatcat caaaaacagg
     1081 caagatgata aaatcagcaa tataattttt ttaaggaata accatgtaaa tggtataggg
     1141 gtttgggaag ggaaggtttt gttatttttt taaaatatgt tagtaaattt atatgctatt
     1201 attttaaaat tatgttatct ttgcaaaaaa tttaatttaa tatgaaaaat attgtatcta
     1261 accttcaaga acacctctcg caaggcaaac aacttaactt agaatttctt aataatctct
     1321 acaataagat gaagctacaa ggcaacgacg tagtagatta tcaacatcta aaagctcaaa
     1381 gtgtctctcc acaatgtcct aattgcaaaa gcattcatat aaagaaaaat ggacatcaac
     1441 aaggccagca aatgtataaa tgtaaaaatt gtggtaaaaa ttttcgtgaa actactggta
     1501 cctttgtcta ctacctgcat aaaaaagaat taatgttaga ctacatccgc ctgatgcttg
     1561 aaggaaaaac cttacgtcaa tgctctaaag aacttcgtat ctctttacct acaagtttcg
     1621 aatggcgaca tcgcattata tctgctctaa gaagtttcga aaataatgta aacttctttg
     1681 gtattatgga aatagatgag ctacaattaa agtactctga gaaaggacgc agatataaaa
     1741 cgttagaaga atatctatta gccaaagagc taaaaactca caatgtagct gtgctatcta
     1801 tgatagacag aagtggtaat atgctatgca aattgacagg tttagataat gtgaaacgag
     1861 atcagataga taaaatttta ttatccagga tatctaataa tgctgtgatt tgctcaccag
     1921 ataatgaaga attcaaaaat ctgaagacac aaagcataaa agaaaaaact ataattactc
     1981 acaggacaaa aaagtatgga cagcatgggt tagcaatagt taaagataag gagaatgatt
     2041 ttgcgggatg gataaattat aaatttagag gggtggctac gaagtatttg cagagctata
     2101 taatgtggta tgtagtgaag cataaatatt tattgagcag agttatggat gatataggta
     2161 ggatgctgaa tttagcagcg tcagactggg aagcatggta tgtatatatg aggttaagag
     2221 taaagcatag ggaaaagcta acataattaa ttattattct ctcccactct ccctcaaaaa
     2281 atttttacaa tcttaattta atttgcctaa attaaattat ctactaatct cggaataatc
     2341 acctatattt agctcatcat atgaatcgtt atagtctgca tatttaccta tcatagaatt
     2401 atcgatattc acatttgaga gtttgacatg ttcttgtaga atagaattag taataataga
     2461 atgtgaaata actgaattat taccaatgct cacatatggt cctacgatag aattctcaat
     2521 aagtacatct ccttctatat aacaaggagg tattataata ccatctttac attttacatt
     2581 atctgcgact acactttcac tcggataacg ttctagaatt cttttatgag caagcaacgt
     2641 agcattctta ttaccacaat ctaaccattc atctacattt tgaattgtta ttttataatc
     2701 attttctatc atatattgta gtgcgtcagt taattgatat tcgccattta cctttttatc
     2761 gttttctaat aattttagta aagatttttt aattatttcg gcatttctga aatagtataa
     2821 acctataata g
//
LOCUS       DAOPRN010000114         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_28890_length_2787_cov_102.484261, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000114 DAOPRN010000000
VERSION     DAOPRN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28890_length_2787_cov_102.484261"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1591)
                     /gene="fumC"
                     /locus_tag="PKI65_08335"
     CDS             complement(200..1591)
                     /gene="fumC"
                     /locus_tag="PKI65_08335"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOB78476.1"
                     /translation="MKKNNFRIEKDTMGELNVAADKYWGAQTERSRSNFKIGDQLMPK
                     EVIEALAYIKKACASANMSFGLLSSQKAELIIAVCDEILQGKLDEHFPLVIWQTGSGT
                     QTNMNINEVIANRAHVLNGGKLDDKNKFLHPNDDVNKSQSSNDVFPSATNIAAYKILV
                     NHTIPTLKKLKDVLNDKSENFKNIIKIGRTHTMDAVPLALGQEFSGYVSQLEHNIQKL
                     EHSLDHLSELPIGGTVVGTGLNAPKTFDVIVCKKIAEFTGLPFKPAKNKFEGISTRDA
                     IVSASGALKSTAVSLFHIANNIRFLASGPRCSIGEINLPANEPGSSMMPGKVNPTQAE
                     ALTMVCAQVIGNDVSINIGGMNGHLQLNTFMPMIIYNFLISAKLITDACISFTDNCVN
                     GIEANEERINMHLNNSLMLATALTPYIGYDKTSLIVKKAFTENKTIKQAGIELGFLTA
                     ENYNKWVNLKKMI"
     gene            1662..2354
                     /gene="rsmI"
                     /locus_tag="PKI65_08340"
     CDS             1662..2354
                     /gene="rsmI"
                     /locus_tag="PKI65_08340"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOB78477.1"
                     /translation="MSYNLYVVPTPIGNLDDITIRGLKYLTNADIIICEDTRSAIMLL
                     KKLEIDVKDKKFLSYHKDNEHKVLDFLINEINKSTFPVLISEAGMPSISDPGFLLIRY
                     CIQNNINIEVIPGASALLVALVGSGLPCDKFHFEGFLPTKSGRKQRLEFIKNYPHTSI
                     IYESPFRINKTLHDLLDFLSPDQPVVICRELTKLYEEYIRGSLSEIIEKTKNKKWKGE
                     IVLIIGSKNFKK"
BASE COUNT          973 a          371 c          373 g         1070 t
ORIGIN      
        1 gcgtggtttt tatacaggga cttgagagct aagccaatat taaatttaac ataaagatct
       61 aaatttatac attattccca aaaatcactt actattcaca ctaataaaat ttataacagt
      121 ttactgtttt acatgctcta atgtgtaagt acaaaaaata tgtttttttc taaatgagag
      181 tattttatta tcttatttgc tagatcattt tttttagatt tacccattta ttataatttt
      241 ctgctgtcaa gaaacctaat tctatgccag cttgttttat ggttttattt tcagtaaatg
      301 cctttttaac tattaaacta gttttgtcat atccaatata tggagttagg gctgtagcta
      361 acatgagaga attgtttaaa tgcatattta ttctctcttc attagcttca ataccattta
      421 cacaattgtc tgtaaaagaa atacaagcat cagttataag tttagcagat atcaaaaaat
      481 tataaattat cataggcata aaagtattaa gttgtaaatg tccattcatg cctcctatat
      541 tgatggatac gtcattgcca attacttgag cacaaaccat agtaagagct tctgcttgtg
      601 taggattcac tttgccgggc atcatgcttg atccaggttc attagcaggt aaatttattt
      661 caccaataga gcacctgggt ccactagcta aaaaacgaat attatttgca atgtgaaata
      721 aactcactgc tgttgatttt agtgctccgc tagcagatac tatagcatca cgagtagaaa
      781 tgccttcaaa tttatttttt gctggtttga aaggtagtcc tgtaaattct gcaatttttt
      841 tacaaactat tacatcaaat gttttaggag catttagtcc agtaccaacg actgtacctc
      901 cgataggaag ctctgataaa tgatctaggg aatgttcgag tttttgtata ttatgttcta
      961 attgtgaaac ataaccagaa aattcttgtc ctaacgctaa aggaactgca tccatagtgt
     1021 gtgtacgacc aattttaata atatttttaa aattttcact tttatcattt aaaacatctt
     1081 ttagtttttt taatgttggt atagtgtggt ttactaagat tttataagca gctatgttag
     1141 tagcagatgg gaatacatca ttagaagatt gacttttatt aacatcatca ttaggatgca
     1201 aaaatttgtt tttatcatct aatttaccac catttaatac atgtgctcta tttgctatta
     1261 cttcatttat attcatatta gtttgtgtcc cagatccagt ttgccaaatt acaagtggaa
     1321 agtgttcatc taatttgcct tgcaaaattt catcgcatac tgctattatt aattcagctt
     1381 tttgactaga taataaacca aaagacatat tagcactagc acaagctttt tttatatatg
     1441 ctaatgcttc tattacttct ttaggcatta gttgatcgcc gattttgaaa ttgcttctac
     1501 tacgttcggt ctgagcaccc caatatttat ctgccgccac attcaattca cccatagtat
     1561 ctttttcgat acgaaaatta ttttttttca tatttttata tttattaaat tatacaagta
     1621 aaattaaaaa aaaattttaa ttttacaaat taaacgaaaa aatgtcttat aatttatatg
     1681 tagttcccac accaattggt aatctagatg atattaccat acgtggatta aaatatttaa
     1741 caaacgctga tattattatt tgtgaagata caagatcagc aattatgttg ttaaaaaaat
     1801 tagaaattga tgtaaaagat aaaaaatttt taagttatca taaagataat gagcataaag
     1861 ttttagattt tttaataaat gaaataaaca aaagtacttt cccagtttta ataagtgagg
     1921 ctggtatgcc tagcattagt gatcctggtt ttttattaat tagatattgc atacaaaata
     1981 atattaatat agaagtgata ccaggagcat cagctttgtt agttgcatta gttgggagcg
     2041 gattgccttg tgataaattt catttcgaag gttttttacc tacaaaatca ggaagaaagc
     2101 aaagattaga atttataaaa aattatccac atacttctat tatttacgaa tcacctttcc
     2161 gtataaataa aactttacat gatttattag attttttgtc tcctgatcaa cctgtagtta
     2221 tctgcagaga attgacaaaa ttgtacgaag aatatattag aggatcttta tcagaaatca
     2281 ttgaaaaaac caaaaataaa aaatggaaag gtgaaattgt attgataata ggtagtaaaa
     2341 attttaaaaa atagtttttg taattttaat ttaaattatg caaaaaaaat aaatattcaa
     2401 tcttaaaata catttatttt attgtcaata tattagcttg aatttttata ttctgttgtt
     2461 ttttaaaaaa atatataata ataatttttt ttatttatct tttttcccaa aagtaaagtt
     2521 caattatcaa taattctttc ttgtgtgtgt tttatttaaa aatactaaat gcataaaact
     2581 agatataaaa taatttttat aataaaattt ttgcgtttta aataaatttt tatgtatatt
     2641 tgtaaaaaaa tagttgtatg aaaaagaata ttttattttt aagcttcttt ttattaaata
     2701 tgtgcttgtt ttctcagaca tatttaataa acaaaaatta ttgcatagtt acttctaatg
     2761 cttatttgat agtaaatggg catttaa
//
LOCUS       DAOPRN010000115         2716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_29859_length_2716_cov_98.234123, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000115 DAOPRN010000000
VERSION     DAOPRN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29859_length_2716_cov_98.234123"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKI65_08345"
     CDS             <1..288
                     /locus_tag="PKI65_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78478.1"
                     /translation="DGIIKSLRTERIIRDDIFSAYELALDRGIDFDVRQIIFDEVPNL
                     SFDDINNFANQYLKNNKYTYSIIGNKEDLPFKDLKKYGKIKELKVSKLLPN"
     gene            complement(537..1733)
                     /locus_tag="PKI65_08350"
     CDS             complement(537..1733)
                     /locus_tag="PKI65_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOB78479.1"
                     /translation="MLSISKKGQMMPPSPIRKLVPYADAAKKRGVKVYHLNIGQPDIE
                     TPKLAIDALKNINLKVIEYSHSAGILSFREKLCKYYEENNIHVTPDEIIVTTGGSEAI
                     LFTFGTIMDNDDEVIIPEPYYANYNGFAINAGVKVVPIMSTIEDNYALPPIEEFEKYI
                     TPKTKAILICNPNNPTGYLYSQEEIEKLGKLVKKHNLFLIADEVYREFVYDGSKHFSV
                     MHLKEIEQNIILVDSVSKRYSACGFRIGMLVSKNKEVMATAMKFAQARLSPPTFGQIA
                     AEAAIDVDKSYFEAIKKEYVARRNVVIEGIKSIPGAYCPTPKGAFYVMAKLPIDDSDK
                     FCQWLLEDFNVNNETVMLAPATGFYSVPERGLQEVRISYVLNVDDMKRSMELLKKALE
                     VYPGRK"
     gene            complement(1810..2178)
                     /locus_tag="PKI65_08355"
     CDS             complement(1810..2178)
                     /locus_tag="PKI65_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOB78480.1"
                     /translation="MKKLIFFFLVFTMMSNLSAQEPMMAFDKTTHDFGKIKITDIVSV
                     DFTIKNIGKAPLFLSEPRSTCGCTVASYPKEPIMPGQSNKISITFEPEKEGPFTKQIT
                     IFSNAENSPITLVIKGEVIK"
     gene            complement(2200..2637)
                     /locus_tag="PKI65_08360"
     CDS             complement(2200..2637)
                     /locus_tag="PKI65_08360"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOB78481.1"
                     /translation="MKTFNFFVLLIFVSQILMAQGNKGPIIKFDNESYNFKEIIQGSK
                     AEIDFVFTNVGDEPLIISNVRSTCGCTVPKWTNEPVKPGEKGKITVKYDSNRIGNFSK
                     QITVSSNANNGNVVLLITGKVSPKPAEAIPYSKTDASGIPIVQ"
BASE COUNT          919 a          425 c          349 g         1023 t
ORIGIN      
        1 gatggtatta taaaatcact tcgcacagag agaataatta gagatgatat attctctgct
       61 tatgaacttg ctttagatag aggtatagac ttcgatgtaa gacaaataat ttttgatgaa
      121 gttcctaatt tatcttttga tgatattaat aatttcgcaa accaatattt gaaaaataat
      181 aaatatactt attccattat tggtaataaa gaagatttac cttttaaaga tctaaaaaaa
      241 tatggtaaaa taaaagaatt gaaagtttct aaacttttac caaactaaat tgtttcataa
      301 ttttataaaa aaaggggcat atgccccttt ttttatacat ctaatttgta catctaatta
      361 atttaccata aatttaattg gtaatgtatc atttacactt tttatatatt ttatcatcca
      421 ttaaatctta attttaaaaa aaattataat aaagtaaaat attttaaatt atttttaaaa
      481 ttctcaaccc attttagtaa ttattatata aaaaagtaag atttcaacaa aaaaatttat
      541 ttccttccag gataaacttc taaagctttt tttagtagct ccatacttcg tttcatatca
      601 tctacattaa gtacatagct gattctcacc tcttgtaatc cacgctctgg tactgaataa
      661 aaaccggtag caggggcgag catcacagtt tcattattta cattaaaatc ttctaaaagc
      721 cactgacaaa atttgtctga gtcatctatt ggcagtttag ccattacata gaaagcacct
      781 ttaggtgtag gacagtaagc tcccggaata ctttttatac cttcgatcac tacattacgt
      841 ctagctacat attctttttt aatagcttcg aaataagatt tgtctacatc tatagctgcc
      901 tcagcagcaa tttgaccaaa agtaggggga ctcaatcttg cttgagcaaa cttcatagca
      961 gtagccatta cttctttatt cttagatact agcataccaa tacgaaaacc gcaagcacta
     1021 tatcgtttag atacagaatc taccaatatt atattttgct ctatttcttt taggtgcata
     1081 acagagaaat gtttactacc atcataaaca aattctctat atacctcatc tgctattaaa
     1141 aataaattat gtttttttac taatttacct aatttttcga tttcttcttg gctatacaaa
     1201 taacctgtag gattattagg attgcagata agaatagctt tggttttagg agtaatatat
     1261 ttttcgaact cctctatcgg aggtagagca taattatctt ctatagtaga cattatagga
     1321 actactttaa ctccagcgtt aatagcaaat ccattataat tagcataata aggctctggt
     1381 atgataacct catcatcatt atccattata gtaccaaagg taaataatat agcttcgcta
     1441 cctcctgtag ttactataat ttcatctggt gtcacgtgaa tattattttc ttcataatat
     1501 ttacataatt tttctctaaa agataatatt cctgccgaat gactatattc tattaccttt
     1561 agattgatat tttttagtgc atctatagct agtttaggtg tttcgatatc tggttgacct
     1621 atgtttaaat gataaacttt gacacctctt tttttagctg catctgcata gggtactaac
     1681 ttccttatag gagaaggggg catcatttgc cctttttttg aaatacttaa catatattca
     1741 ttgttttata attgttaatc actttttttt taaaaaaaaa tattatttca ttgtttaatt
     1801 ttttcatttc tatttgatca cttcgccttt gattaccagc gtgataggag aattttcggc
     1861 attagaaaaa atagttattt gtttagtaaa aggcccttct ttttcgggtt cgaaagttat
     1921 actgatttta ttagattgtc ctggcataat aggttcttta ggatatgaag ccacagtaca
     1981 tccacaagta gaacgcggtt cagataaaaa caaaggagct tttccaatat ttttaatagt
     2041 aaagtctacg ctcactatat ctgttatttt gattttgcca aaatcatggg ttgttttatc
     2101 aaatgccatc ataggctctt gagctgataa gttactcatc attgtaaaaa ccaaaaaaaa
     2161 gaaaatcaat tttttcatga taatagtttt tttttataat tattgaacta ttggaattcc
     2221 agaagcgtca gttttactat acggtatagc ttcagcaggt tttggactta ctttgccagt
     2281 gattagtaaa actacattac cattattagc attagagctt acggtaattt gtttcgaaaa
     2341 attaccaatc ctattagaat catacttaac agtgatttta cctttttcac ctggtttaac
     2401 aggttcatta gtccatttag gtactgtaca accgcaagta ctacgtacat tagatattat
     2461 gagaggttca tctcctacat ttgtaaaaac aaaatctatt tctgcttttg agccctgaat
     2521 tatttcctta aaattatatg attcattatc aaatttaata ataggccctt tattaccttg
     2581 tgccattaat atttgagata caaatattaa taaaacaaaa aaattaaatg ttttcatatt
     2641 tttcttacaa atttaatgat tatttaaaat ttagcaaaaa ttataccata aaatcacatt
     2701 tacttaaata aaaatt
//
LOCUS       DAOPRN010000116         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_31294_length_2616_cov_145.967591, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000116 DAOPRN010000000
VERSION     DAOPRN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31294_length_2616_cov_145.967591"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..701
                     /locus_tag="PKI65_08365"
     CDS             147..701
                     /locus_tag="PKI65_08365"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOB78482.1"
                     /translation="MSKSYESINQQQIEQFILFCQDYCNLIESAEEYDFENIVNFLLV
                     ALPMLYFYGTIINLIDDADIEYAERTVNEETYTIIYNRLNEIFSKYFDFQITDDDYLW
                     INDISIPEFLSDIYQDLKDVVVLYNKNKLETQKAAIYLAKYWFIDRWGKESLKVLLAL
                     HSYNYRYEDGTDNNFYNSDDIYNL"
     gene            751..2253
                     /locus_tag="PKI65_08370"
     CDS             751..2253
                     /locus_tag="PKI65_08370"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HOB78483.1"
                     /translation="MKKFFVLSILSFLLMINVALSQKNPGYKIQVTIPQLADTNVYLG
                     YYYGKWQYVKDTIKLDKAGKGIFQSKDTVGSGMYLLITPSKKYLEFILNDNERIFSIE
                     TDTTDLLGKVKVKGSLENELFFRVNNEIAKPSKEIYSLQQKVKEFKQKNLSDSVEFYN
                     SRIKLLIDSINNMKIKYMEQYPTTLLARMFKATKEPEVPDKLPLKADGSVDSSYKYYY
                     FKDHYWDNINIADEALLRTPIYHNKLETYFNNVIVQHPDTIIKEIDKILAKAAANKET
                     FKYVLWWLTYNYETSKVMGFDKIFVYLADNYYKTGKAYWLSESSTQKIIERADAIRPN
                     LLGNIAPNLILLDTAKNLNSLHNINAKYTILYFWDPSCGHCKKETPKLVEFYKQYKDS
                     FNLKVFAVCTDSSFIEMKKVINNMQMQDFINVNGIYTASGDFRKYYDIYSTPVMYVLD
                     QYKRIIGKRLITDQLYDFLKNYEKHPLFPEPPEQSKEPYIPLMPGGSFNE"
     gene            2377..>2616
                     /locus_tag="PKI65_08375"
     CDS             2377..>2616
                     /locus_tag="PKI65_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOB78484.1"
                     /translation="MDFIAELEWRGMIYEMIPEVKNYLSAPGAAAYLGIDPTADSLHI
                     GHLVSIMLLRHFQRAGHKPILVLGGATGMIGDPSGK"
BASE COUNT          997 a          316 c          399 g          904 t
ORIGIN      
        1 gattgcaatt tttacagttt tcataactct ttttttgcaa aattaatcaa aaattattag
       61 tctgacaaac taatttatat cattctttac tgaatatatt tgggctaaaa ttaaaaaatt
      121 aattattttt gtaaaaacat ctttttatgt ctaaaagcta tgaaagcata aatcagcaac
      181 agatagaaca atttatctta ttttgtcaag attactgtaa ccttatagaa tctgctgagg
      241 aatatgattt tgaaaatatt gtaaattttt tactagtggc tctgccaatg ctatatttct
      301 acggtacaat aattaattta atagatgatg cagatatcga atatgctgag aggacggtca
      361 atgaagaaac ctacactata atttacaata gattaaatga gattttttct aaatattttg
      421 attttcaaat aactgatgat gattatttgt ggataaatga tatttctatt cctgagtttc
      481 taagtgatat atatcaagat ctaaaagatg ttgttgtttt atataataaa aataaattag
      541 aaactcaaaa agctgcaatt tatttagcta aatattggtt tatcgataga tggggtaaag
      601 aaagtctcaa agtgctttta gctttacata gttacaatta tagatacgaa gatggaactg
      661 ataataattt ttataatagc gatgatattt ataatttgtg attttttaat aaaaaaataa
      721 taaatttgca ttataaaaaa aataagaaag atgaaaaagt tttttgtact atcaatcctt
      781 agctttttat taatgataaa tgtagcttta tcacaaaaaa atccgggtta caaaattcaa
      841 gttacaattc cacaattagc cgatactaat gtgtatctcg gatattacta tggtaaatgg
      901 caatatgtaa aagacactat taagttagat aaggctggca aagggatatt tcaatctaaa
      961 gatactgtag gtagcggaat gtatttgctc ataacaccat ctaagaaata tttagaattt
     1021 attctaaatg acaatgaaag aatttttagt atagaaactg atactacaga tttactgggc
     1081 aaagttaaag taaaaggctc tttagaaaat gaattatttt ttagggttaa taatgaaatt
     1141 gctaagccat caaaagaaat atattcttta cagcaaaaag taaaagaatt taagcaaaaa
     1201 aacttgtcag attcagtaga attctataat tctaggatca aattactcat agatagtata
     1261 aacaatatga agataaaata tatggagcaa tatcctacaa ctcttttagc taggatgttt
     1321 aaagctacta aagaaccaga agtgccagat aaattacctt taaaggcaga tggtagtgtc
     1381 gatagctcct acaaatatta ttattttaaa gatcactatt gggataatat aaatattgcc
     1441 gacgaggctc tgctgcgtac acctatttat cataataaat tggaaactta ttttaataat
     1501 gtcattgttc agcatccaga tacaattatc aaagaaattg ataaaatatt agcaaaagca
     1561 gcagctaata aagaaacctt taagtatgta ctatggtggt taacttataa ttatgaaact
     1621 agtaaagtta tgggctttga caaaatattt gtttatttag cagacaatta ctataaaact
     1681 ggtaaagctt attggctatc agaatcttct acacaaaaaa ttatagagag agcagatgct
     1741 atacgaccta atttattggg taatattgcg cctaatctga ttttattaga tactgctaaa
     1801 aatttaaatt cattacataa tataaatgct aaatatacaa tattatattt ttgggatccc
     1861 agctgtggac attgtaaaaa ggaaacacca aaattagtag agttttacaa acagtataaa
     1921 gattcattta atttaaaggt atttgctgta tgcactgata gtagctttat agagatgaaa
     1981 aaagtaatta ataatatgca aatgcaagat tttataaatg taaatggcat ttatactgca
     2041 tcaggcgatt ttagaaaata ttatgatata tattctacac cagtgatgta tgttttagat
     2101 caatataaaa ggataatagg taaaaggttg ataacagatc aattatatga ttttttaaaa
     2161 aattatgaaa agcatcctct ttttcctgaa cctcccgaac aatcaaaaga accatatatt
     2221 ccacttatgc caggtggtag ctttaatgaa tgaaaaaatt tttgttaaaa ttttgctcca
     2281 gaaactttat catgcaaaaa gtattataat cagccactaa atttattatt ttaaatttgt
     2341 atttttgcaa tttaaattaa aaaattgttt ttcattatgg atttcattgc tgagttagag
     2401 tggagaggta tgatttatga aatgatacca gaggttaaaa attatttatc tgctcctggt
     2461 gccgcagcat atttgggtat agatcctaca gcagattctt tgcatatcgg tcatttagta
     2521 tcaataatgc ttttgagaca ttttcagaga gctggtcata aacccatatt agtcctgggt
     2581 ggtgctactg gcatgatagg agatccttct ggcaaa
//
LOCUS       DAOPRN010000117         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_33029_length_2507_cov_106.709625, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000117 DAOPRN010000000
VERSION     DAOPRN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33029_length_2507_cov_106.709625"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2065)
                     /locus_tag="PKI65_08380"
     CDS             complement(<1..2065)
                     /locus_tag="PKI65_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78485.1"
                     /translation="MKKVLVYLFLFNVISFAFAQDYSVVNIKVEDSQNHNNIATAEVS
                     IGEFKSTTNEEGIASFKILPGIYGLYVNATGYQPYNDIIDLMSVKDTTIVILLTRGLS
                     QTVQQDLARELELEIVNDDLDRGISLSPLLLSSRDPYLNLASYNLSAGSFSPRGIERG
                     GYESYINQVLLYNPELDRVNYSDISGLNDATRYSDDSYGLEPLYMSFGDIMGVSNILA
                     TPSRIRLGHQISYGISNKSWEHRLMYTYNSGIMQNGWGFAAAYSRRYAYDTYMPGTWL
                     QADAFFFSAEKRFSERHSLSATIFASPQKRALASPTVKEAQELVDDPYYNPNWGVQNG
                     EKRNARVRTTFKPYLILNDEYKFNDNLTWTNAFGFTTGIYGTTRLNWYNAPDPRPDYY
                     RYLPSYQTDPFIAEIVTQRWQNDVTVSQINWDKLYQINYLANLDGKSARYIVEEVHND
                     PTYFYYASNIKYTFNPSSIIYGGLQAYHYETHNYKKINDLLGANYYLDIDQYAERDFP
                     DKPEMLYNNLDDPNRQLYVGDVFGYNYKLNTNDVNLWTQYKQNLPRVDYFVAGKIGFS
                     NKYRNGLFRSGRFPDNSKGKSDQINDLVGGLKTGATFKISGKHYIKANIAGFTKPQSS
                     LDIFLSPYNSNRINDDITTQKVLSGDIGYHFNFKFLRGRITAYQTYILDHMDMRGFYH
                     DEYQTY"
     gene            complement(2122..>2507)
                     /locus_tag="PKI65_08385"
     CDS             complement(2122..>2507)
                     /locus_tag="PKI65_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOB78486.1"
                     /translation="AKGVLLQPMLSGLELFIGGKKEGDFGHIVTAGLGGIFVEVLKDI
                     QTALSPITFDEALTMIHNLKGYKLIKGVRGSKGVDENVFANIIQRVSALMTIAPEIAE
                     MDINPLLGNGSNIIAVDVRINIIKN"
BASE COUNT          865 a          442 c          355 g          845 t
ORIGIN      
        1 cgtatgtctg gtattcatca tgataaaaac ctcgcatatc catatgatct aatatgtagg
       61 tttgataagc agtgatgcga cctcgtaaaa atttaaaatt aaaatgatat cctatatcac
      121 cagataaaac tttttgtgtt gtaatatcat cattgattct attactatta tatggagaaa
      181 gaaaaatatc tagactagat tgcggttttg taaatccagc aatattagct tttatataat
      241 gtttacctga aattttaaat gtagcaccag tttttaaacc acctacaagg tcatttattt
      301 gatcagattt ccctttacta ttgtcgggaa atctaccact tctgaataaa ccatttcgat
      361 atttattgct aaaacctatt ttaccagcta cgaaataatc aactcttggc aaattttgct
      421 tatattgtgt ccataagttt acatcattag tgtttagttt ataattataa ccaaaaacat
      481 cacctacgta cagttgtcta ttagggtcat ctaaattatt ataaagcatc tctggcttat
      541 ctgggaaatc tctttctgca tattgatcga tatctaaata ataattagca cctaataaat
      601 cgttaatttt tttataatta tgagtttcgt aatgataggc ttgtaaaccg ccataaataa
      661 tgctagatgg attaaaagta tatttgatat tgctagcata atagaaatag gtaggatcat
      721 tatgtacctc ttctacaata tatctagcag atttcccatc taaattagct agataattaa
      781 tttgataaag tttatcccaa ttaatttggc taacagtaac atcattttgc catctttgtg
      841 taactatttc tgcaataaat ggatctgttt gatagctcgg taaatatcta taatagtctg
      901 gtctaggatc aggagcatta taccaattta gtctagtagt accataaata cctgttgtga
      961 aaccaaaagc attagtccat gttagattat cattaaattt atactcatca tttagtatta
     1021 aatatggttt aaaagtagtc ctaactctag cattacgttt ttcgccattt tgtacacccc
     1081 agttagggtt atagtaaggg tcatcgacta attcttgtgc ttctttaaca gtaggagaag
     1141 ccaaagctct tttttgtgga ctggcaaaga tagtagcaga caagctatga cgttctgaaa
     1201 aacgtttttc tgctgaaaag aaaaaagcat ctgcttgtaa ccaagtgcca ggcatataag
     1261 tatcataagc atatctacga gaataagcag ccgcaaagcc ccacccattt tgcataatgc
     1321 cactattata tgtatacatt aatctatgtt cccatgattt attactaata ccgtagctga
     1381 tctgatgtcc gagcctgatg cgagatggag tagctaaaat attagatacg cccataatat
     1441 cgccgaagct catatataat ggctctaatc cataggaatc gtcagaatat ctagtagcat
     1501 catttaatcc actaatgtca gaataattaa ctctatctag ctctggatta tatagcagca
     1561 cttgattaat atatgattca taaccaccac gttctatgcc acgaggtgaa aatgaacctg
     1621 cacttaaatt gtaagaagct aagttaagat atggatctcg agaactaaga agcaatggac
     1681 ttaatgaaat gcccctatca agatcatcat ttactatctc taactctagc tctcgagcta
     1741 aatcttgttg aacagtttga gataaacccc ttgtgagcaa aattacaata gttgtatctt
     1801 tcacagacat tagatcgata atgtcattat aaggctgata acctgtagca ttcacataaa
     1861 gaccataaat accaggtaaa attttaaatg aagcaatgcc ctcttcatta gttgttgatt
     1921 taaattcacc aattgatact tcagcagtag caatattatt gtgattttgt gaatcttcta
     1981 cttttatatt tacaacagaa taatcctgtg caaatgcaaa actaatcaca ttgaataaaa
     2041 aaagatatac caaaaccttt ttcataaaac tgaattaatt atgcaaaaat aaataaaatt
     2101 ttaaaacaaa cacacaaaat attaattttt aataatattt attctaacat ccactgctat
     2161 tatattagag ccattgccca ataaaggatt tatatccatt tcagcaattt caggagctat
     2221 agtcattaga gcacttacac gttgtataat atttgcaaag acattctcat ctaccccttt
     2281 actacctcta acacctttta ttagtttgta acctttcaaa ttatgtatca ttgtcaaagc
     2341 ttcatcaaat gtaatgggtg atagagcagt ttgaatatct ttaagaactt ctacaaatat
     2401 accacctaac ccagcagtga ctatatgtcc gaaatcacct tcttttttgc caccaataaa
     2461 taattctaat ccactaagca taggttgtaa aaggacacct ttagcat
//
LOCUS       DAOPRN010000118         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_35595_length_2367_cov_108.638841, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000118 DAOPRN010000000
VERSION     DAOPRN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35595_length_2367_cov_108.638841"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PKI65_08390"
     CDS             <1..461
                     /locus_tag="PKI65_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78487.1"
                     /translation="IASGFDKLDPYARFGVIFGFGSINLNYLDEEYENNQLEDKEVVK
                     WKMDGGMAFGISSALGLMYHISDLISVYGELNLVGMSYAPKKGVMTEYTINGTDQLPG
                     LTTDDKEIDFVDDITYDYDNPPSSAEPMKELKFYFPYSSIGLNIGVRFSF"
     gene            540..1178
                     /locus_tag="PKI65_08395"
     CDS             540..1178
                     /locus_tag="PKI65_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOB78488.1"
                     /translation="MKKVNLVIVIILSFILSINAFSQNLSIGAKGGLNLSNMIVKNDD
                     ETMSDDFKMLAGFHVYPFVEYSFSDLFAIDAGLNFQSRGYSIIEDNFKYRFTTLYIDI
                     PINAKFNFDLGKVKLFCNVGPYVAFGIGGTVYAETTIDRHTISARRDIDWGDDLKSID
                     FGLNIGVGLDINNFIVGLNYGGGIADLSTIDDDDSRTNNYVLSISLGYKFNL"
     gene            1231..1872
                     /locus_tag="PKI65_08400"
     CDS             1231..1872
                     /locus_tag="PKI65_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOB78489.1"
                     /translation="MKKIFIFSFVMIISFTNAIFSQNISIGARAGLNLSSMFIKEGSY
                     SFNDNLSMLTGFHINPYVEYSFSDKFALEGGLSFQTRGFIFNETDMIRQKITPHYLDI
                     PINAKFYFNIGKPKIYVYVGPYLGIGLFGKEKQYRNDALLSERTLEWGQYIPADLNRI
                     DFGINGGVGIDINNFSIGAQYGYGFLNVSTLDNYGIKMHNYVINISIGYKFDL"
BASE COUNT          821 a          249 c          433 g          864 t
ORIGIN      
        1 taatagctag tggttttgac aagctagacc cttatgctag atttggtgtg atatttggct
       61 ttggatctat taatttaaat tatttggatg aggaatatga aaataatcaa ttagaagata
      121 aagaagtagt aaagtggaaa atggatggag gaatggcttt tggtatttca tcagctcttg
      181 gacttatgta tcacatatca gatttaatat ctgtttatgg agaattaaat ttagtaggta
      241 tgagctatgc tcctaaaaaa ggtgtaatga ctgaatatac cattaatgga acagatcagt
      301 tgccaggatt gacaacagat gacaaagaaa ttgactttgt agatgatatt acttatgatt
      361 atgataatcc tccatcatca gcagagccta tgaaagaact aaaattttat ttcccttata
      421 gcagcatagg acttaatatt ggagttagat tttcattcta gttgatttag tttttgttaa
      481 aatatcttaa aaaagcagaa ttttttattc tgatattttt ttatctttga aaaaaaatta
      541 tgaaaaaagt taatttagtt atagttatta ttttatcttt cattttgtcg ataaatgctt
      601 tttcacaaaa tttgagtata ggtgctaaag gtggattaaa tctatctaat atgatagtta
      661 aaaatgatga tgagaccatg agcgatgact ttaagatgct tgccggtttt catgtttatc
      721 ctttcgtaga gtattctttt tcagatcttt tcgcaataga tgcaggctta aattttcaat
      781 ctcgtggtta ctcaattata gaggataatt tcaagtatcg tttcaccact ctgtatatag
      841 atatacctat aaatgctaag tttaattttg atttaggtaa ggtaaaatta ttttgtaatg
      901 tgggtcccta tgttgcattt ggtatagggg gcacagttta tgcagaaact actatagata
      961 gacatacaat ttcggccaga agagatatag actggggcga tgatttgaaa tctattgatt
     1021 ttggactaaa tataggtgtc ggtctcgata ttaataattt tattgttggt ttgaattatg
     1081 gcgggggaat agcagaccta tctactatag acgatgatga cagcagaact aataattatg
     1141 tgttaagcat atctttaggg tataaattta atctatagat gcttatgatt ttctcaaaat
     1201 aaataaatta attatttttg aaaaaaaatt atgaaaaaga tttttatttt tagttttgta
     1261 atgattatat catttacaaa tgcaatattt tcccaaaata ttagcatagg agcaagggct
     1321 ggcctaaatt tatcaagtat gtttataaaa gagggcagtt atagttttaa tgataattta
     1381 agtatgttga caggatttca tataaatcct tatgtagagt attctttttc agataaattt
     1441 gctttagagg gaggtttatc attccaaact cgtggattta tttttaatga aacagatatg
     1501 ataagacaaa aaatcacacc tcattactta gatataccca ttaatgctaa attttatttt
     1561 aatataggta aacctaaaat atatgtttat gttggaccat atttaggtat aggcctattc
     1621 ggtaaagaaa agcaatatag aaatgatgct ttattaagtg aaagaacgct agagtggggg
     1681 caatatatac ctgcagattt aaatagaata gattttggta ttaatggtgg agtcggaata
     1741 gatattaata attttagtat aggtgctcaa tatggctatg gatttttaaa tgtttctaca
     1801 ttagataatt atggtatcaa aatgcataat tatgttatta acatatctat agggtataaa
     1861 tttgatctat agaattaggt gtttggttaa taattgattg ggaaatttgg ttaatgtgta
     1921 gtgagatgac tgggagacta tgagacgggg agactgggtg aggtggagaa ggggagaaaa
     1981 ggagattatt gattagttca gaagttgttg agcaaaatgc tatttgtctg ttgttattaa
     2041 tccacagatg tacacaaatg agcacaaatt aaagtttaat attgatgtgc tgtttaaagg
     2101 gatgtccaaa tagtagtttt ataaaattat atccttagcg aagacttttt ttgttatttt
     2161 tctttgtgta gctgtcaaag aaaagtaaat taagtagaaa tttatttaaa atctattata
     2221 ctcataagaa atatagctat taacgtaagt ttaccagtaa taaattagtt attaattaaa
     2281 cgttcaattc aacaagataa cttcaaaaca atgaaaaaac tttaaagaat agagttattc
     2341 ttttctatat actttgctca caccatc
//
LOCUS       DAOPRN010000119         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_35925_length_2350_cov_111.010893, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000119 DAOPRN010000000
VERSION     DAOPRN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35925_length_2350_cov_111.010893"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1631
                     /locus_tag="PKI65_08405"
     CDS             <1..1631
                     /locus_tag="PKI65_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOB78490.1"
                     /translation="ILNTKPFSQTKTKATVKINKMLIDTQWVNTNGKVLLYLNLPIDS
                     FNLTNHSIIFRNSLQSPNEIKFPYEFDYAKYLANLQIYHIGYINDYDFKIIERKSNLL
                     EKFILFRNKLIKLIYEKITDNSSANLISAMLLGYQDVMDADIIKDFQQAGVVHILSIS
                     GLHVAIIYGAIYLLFSLIIPLKYKNKITIPLAIITLWLYTLMVGAMPSVVRSAIMLTL
                     LQLGPVINRNVNNYNSLAAAALIILVINPLQLYSLGFQLSFSAVLGIFFFYTPLINIF
                     KTNKKWLKWLIGSLSVTMAAQLGTLPFTLFYFHQFPTYFIIANLIAVPFASLLIYAGF
                     ITIIFAFIPVLGGFIAMIFSLLVKIFINFIGIVSNLPYSTIPNIFLTPLSAIMIGIII
                     ILITIKIYFKEIKIFPYLLTAVLLFGITYNFSLIKRIRYNPSFESLNYKYAIIGQKEQ
                     EKFNYIVIKPDSLDLPNIQPKIDDFCTYYFCKEKNKYEINEKVKTHSFYINNPIISNA
                     DNVYVIGNKNEINSLIDSLDFKKIIIDKRKLKFQ"
     gene            complement(1695..2030)
                     /locus_tag="PKI65_08410"
     CDS             complement(1695..2030)
                     /locus_tag="PKI65_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOB78491.1"
                     /translation="MKEKIFNFFHYKITNRSIENYFNVLNNDTIRIERIISFGQQSPP
                     DFWYDQDEDEWILLLKGKAKLEFIDRKITLNKGDSLLIPAHEKHRVAYTHKYKPTIWL
                     TVFFKSKSI"
     gene            complement(2011..>2350)
                     /locus_tag="PKI65_08415"
     CDS             complement(2011..>2350)
                     /locus_tag="PKI65_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOB78492.1"
                     /translation="NNKIPKILENRRKTIGIRIPDNKIVHAIVDTIGHPIVSSSIKVN
                     DQIGNEYISDPELIYEMYMDIVDAVIDGGPGGLIPSTIIDATGEIPVLVRKGAGPVNF
                     LDLLDEGENI"
BASE COUNT          877 a          303 c          284 g          886 t
ORIGIN      
        1 atatactaaa tacaaagcct ttttctcaaa ctaaaactaa agcaacagta aagataaaca
       61 agatgctaat agatactcag tgggtcaata ctaatggtaa agtattatta tatcttaatt
      121 tgcccattga tagttttaat cttacaaatc attcgattat atttagaaat tctttgcaat
      181 cacctaatga gataaaattt ccatatgaat ttgattatgc taaatattta gcaaacctgc
      241 aaatatatca tatcggttat ataaatgatt atgactttaa aattattgaa cgtaaaagca
      301 atttactaga aaaattcata ttatttagaa ataaattaat aaaattaatt tatgagaaga
      361 ttacagataa ctcatcagct aatttaataa gtgcaatgct attaggatat caggatgtaa
      421 tggatgcaga tataataaaa gactttcagc aagcaggagt tgtacatatt ttatctattt
      481 ctggactgca tgtagctata atctatggag ctatatattt gctattttca ttaataattc
      541 ctttaaaata taaaaataaa attacaatac cattagctat aataacatta tggttatata
      601 cattaatggt cggagctatg ccgtcagtag tgagatctgc tattatgctt acattattac
      661 aattaggccc agttattaat agaaatgtga ataattataa ttcattagca gcggcagcat
      721 tgatcatatt agttattaat ccattacaat tatattcatt aggttttcag ttatcttttt
      781 ctgctgtatt aggaatattc tttttttata ctccattaat aaatattttt aaaactaata
      841 aaaaatggct gaaatggtta ataggttcat tatcagttac gatggctgcg cagttaggca
      901 cattaccatt cactttgttt tattttcatc aatttcctac ttattttata attgcaaact
      961 taatagcagt gccatttgct tcattattga tttatgctgg atttattaca ataatattcg
     1021 catttatacc tgtattaggt ggatttattg caatgatatt ttcactctta gttaaaatat
     1081 ttataaattt tattggaata gtatctaatc taccttattc tactatacct aatatttttt
     1141 taacaccatt gtcagcaata atgataggaa taataataat attgataaca attaaaattt
     1201 attttaaaga aataaaaatt tttccatatt tattaactgc tgtattactt tttggtataa
     1261 catataattt ttctttaata aaaagaattc gttacaatcc atcttttgaa tcattaaatt
     1321 ataagtatgc aattatcgga caaaaagaac aagaaaaatt taattatatt gttattaaac
     1381 cagattctct agatttacca aatattcagc ccaaaataga tgatttttgc acatattatt
     1441 tttgtaaaga aaaaaataaa tatgaaataa atgaaaaagt aaaaacgcat tcattttata
     1501 ttaataatcc tataatatct aatgcagata atgtatatgt aataggcaat aaaaatgaaa
     1561 taaatagcct gatagatagt ttagatttta aaaaaataat aatagacaag agaaaattaa
     1621 aatttcagta atataaagca tttataataa attagttgat taaatcttat tggattgagc
     1681 tatatattaa acttttagat agatttactt ttaaaaaaaa ctgttaacca aatagtgggt
     1741 ttgtatttat gagtataagc tactctatgt ttttcatgag cagggatgag taaggaatcg
     1801 cctttgttaa gagtaatttt tctatctata aattctaatt tagccttacc ttttagtagc
     1861 aatatccatt catcctcatc ttgatcatac caaaaatctg gaggactttg ttgaccaaaa
     1921 gaaataattc gttctattct aatggtatca ttatttaaaa cattaaaata attttcaatg
     1981 gatctatttg taatcttata atgaaaaaag ttaaatattt tctccttcat ctaataaatc
     2041 taaaaaatta actggtcctg ccccttttct aactaaaaca ggtatttcac cagtagcatc
     2101 tattatagta ctaggtatta gaccacctgg acctccatct atgacagcat ctactatatc
     2161 catatacatt tcataaatga gttctggatc tgaaatatat tcattaccta tttggtcatt
     2221 tacttttata gaagaagaaa ctatgggatg cccaatagtg tctacaatag catggacaat
     2281 tttattatct ggtatacgta ttccaatagt ttttctacga ttttctaata ttttaggtat
     2341 tttattatta
//
LOCUS       DAOPRN010000120         2294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_37073_length_2294_cov_105.215721, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000120 DAOPRN010000000
VERSION     DAOPRN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37073_length_2294_cov_105.215721"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..653
                     /gene="coaD"
                     /locus_tag="PKI65_08420"
     CDS             153..653
                     /gene="coaD"
                     /locus_tag="PKI65_08420"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOB78493.1"
                     /translation="MKKENRAVFPGSFDPITRGHKDVICRAVPLFDKIYVAIGQNANK
                     KYMFPLDKRLQWLQIIFKNIPTIEVSCFDGLTIDFCKKHNINFILRGLRTSADFEFER
                     QIAQINKQLDNEIETIFLLTHPIYTPLNSSIVREIISFNGDISNFVPEDILNDIITTY
                     QNIYNT"
     gene            613..2076
                     /locus_tag="PKI65_08425"
     CDS             613..2076
                     /locus_tag="PKI65_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HOB78494.1"
                     /translation="MILLLLIKIFIIPKRYFYMRIYFFIFYFFLSFFVFSQVTTIEGN
                     FKSAQGKWLRLYAVQDFITYSDTLLAKTKIDSSGNFLINIKVPKTKVYYAYFKVNDLR
                     SNDLYIEENKKYYIVFDSLNFEILDTYTSFLTSLRLYFELQNVDTNDLNYKITQLNIM
                     LNKFYAENIKVESERAGSLLNKIPKDKIDSLESILYTKFANYNHPYFRDYLQYAIAEI
                     KLISRSYDKLEIFNQYLWSHRPIYDNVQFMSFFINYFKDFIINSNKIKNRDLISNIEW
                     KVNYTALLDSLGKDTLLRNEVTRELVLLQNIVDWANMKLFTIDSLKKLLDQFYINTKF
                     DHHRLIIRNIQRNMFTINRVDLSEIPLLSKNNDTIFLKQFNTDFILLYFFTSWCRPCI
                     KELVTLDAIAPDICNKIKIIAISADRIPINFYYFTQDYSLQNIDLYYFNQHYELLENL
                     KIVVYPQSILLNKEGYIINAAMPDIKNGLIEYLYNIF"
     gene            2156..2229
                     /locus_tag="PKI65_08430"
     tRNA            2156..2229
                     /locus_tag="PKI65_08430"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2190..2192,aa:Met,seq:cat)
BASE COUNT          830 a          280 c          310 g          874 t
ORIGIN      
        1 aaatattgga acatattttt gcaaatagtg gcagatttat taaatggagc caaatactaa
       61 aattaaagaa tgatatatta taatgtatga taaatgtaga aaataaattt ttatattaca
      121 aactttattt aattttgttt aaatattttt ttatgaaaaa agaaaatcgt gctgtttttc
      181 caggttcttt tgatccaatt actcgagggc acaaggacgt tatatgtaga gctgtacctt
      241 tatttgataa aatttatgta gctattggcc aaaatgctaa taaaaaatat atgtttcctc
      301 ttgataaaag attgcaatgg ttacagatta tttttaaaaa tattcctaca atagaggttt
      361 cttgtttcga tggtttaaca atagatttct gtaaaaaaca taatatcaat tttatattaa
      421 gaggtctgcg aacctcagct gatttcgaat ttgaacgcca aatagcacag ataaataagc
      481 agctcgataa tgaaattgag acaatttttc ttttgacaca tcctatttat actccactaa
      541 attcatctat cgttagagaa attattagtt ttaatggtga tattagtaat ttcgtgccag
      601 aggatatttt aaatgatatt attactactt atcaaaatat ttataatacc taagcgttat
      661 ttttatatgc gcatttattt ttttattttt tatttttttt taagtttttt tgtttttagc
      721 caggttacaa cgatagaagg taatttcaaa agtgctcaag gtaaatggct tagattatat
      781 gctgtgcaag atttcattac atactccgat actttgttag ctaaaactaa aattgattct
      841 tctggcaatt ttttaattaa tatcaaagtg cctaaaacta aagtttatta tgcttatttc
      901 aaagttaatg atttacgtag caatgatcta tatatcgaag aaaataaaaa atattatatc
      961 gttttcgatt cactcaattt tgaaattttg gatacatata cttcattttt gactagtttg
     1021 cgtctttatt ttgaattaca gaatgttgat actaatgatc tgaattataa aattactcaa
     1081 ttgaatataa tgctaaataa attttatgct gaaaatataa aagttgaatc tgaacgagcg
     1141 ggtagtttat taaataaaat acctaaagat aaaattgata gtctagaaag catattatat
     1201 actaaatttg caaattataa tcatccatat tttcgcgatt atttacaata tgctattgca
     1261 gaaatcaaat taatttcaag atcttatgat aaattagaaa tttttaatca atatctatgg
     1321 tcgcaccgcc ccatttatga taatgtgcaa tttatgagct tctttattaa ttattttaaa
     1381 gattttatta ttaattccaa taaaattaaa aatcgagatt taatttctaa tatagaatgg
     1441 aaagttaatt acactgcctt attagattct ttaggtaagg atacgttatt acgaaatgaa
     1501 gtaactcgag agctagtgct tttgcaaaat attgttgatt gggctaatat gaaattattt
     1561 actatagata gcttaaaaaa attattagat caattttata taaatactaa atttgatcat
     1621 catcgactta tcatccgaaa tattcaacga aatatgttta ctattaatag ggtagattta
     1681 tctgagattc cacttttgtc taagaataat gatactattt ttttaaaaca atttaatact
     1741 gattttatac tattatattt ttttacttct tggtgcagac cttgtattaa agaattagtg
     1801 actctagatg ccatagctcc agatatttgt aataaaataa aaataatagc tatctctgct
     1861 gatagaattc ctattaattt ttattatttt acccaagatt attcacttca aaatatagat
     1921 ctttattatt ttaatcaaca ttacgaatta ttagaaaatt taaaaattgt tgtttatccg
     1981 caatctattt tattaaataa agaaggctac ataataaatg ctgctatgcc cgatatcaaa
     2041 aatggcctaa tagaatattt atataatatt ttttaagcta acataattga ttaaaaacaa
     2101 taaaaaatat aattttttat attataaaaa tttattagtt ttgcaaaaat aaattggcgg
     2161 gatagctcag gtggttagag cgacggattc ataacccgta ggtcgcgagt tcaactctcg
     2221 ctcccgctat taacatagaa aaattacttt acggggcgta gcgcagttgg ctagcgcact
     2281 tgcatggggt gcaa
//
LOCUS       DAOPRN010000121         2200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_39106_length_2200_cov_128.780886, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000121 DAOPRN010000000
VERSION     DAOPRN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39106_length_2200_cov_128.780886"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(431..1849)
                     /locus_tag="PKI65_08435"
     CDS             complement(431..1849)
                     /locus_tag="PKI65_08435"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOB78495.1"
                     /translation="MQHLMKSLKLILLILFSSFFISVNAQNISGTIEHASNISLYLFS
                     YYGNEQKLIDSSITDDKGNFNFKMPDTGIQYEVRLGNSSQFFYILKDENDVKLKTVYN
                     PNQMWNYAEDSMKIIKSKENKEFYKFLKYQRSWMISQYWLLQMLRLYPIIDPFHKTLV
                     QEYNDRYENLANFMEKSKKNLKYNAHRIALAYYLPNPDWKMPDPWRDSVMAAHFFDNF
                     DPSDPIFLYTFVLPEKMEMYMKLKTNIRDEYGQPVYDEFLFASAALDFLKHTEKNLAV
                     RNFCLSYFLNFFKDQHLEKAFIIVYDEYLKTEEGDCGSQDDQWAWARKLADKYKGIEL
                     GNPAPDFVIPQKNISLYDLPTKYILLVFWASWCPHCTEEIPYIYETTMDFPDSTLTTV
                     AISLDTVPQMLETFIDIKNIKNRWIHYSEFKGWNSNVVKLYNVYATPTMFLLDKDRKV
                     IAKPISYDELTKVLIKLKRGEL"
     gene            1990..>2200
                     /locus_tag="PKI65_08440"
     CDS             1990..>2200
                     /locus_tag="PKI65_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOB78496.1"
                     /translation="MLGTYNIERKLNFATLEDIMYHRVDVQLSEVAKNNIQKSRQFLD
                     NYLEKNDNPVYGINTGFGALYSVKIP"
BASE COUNT          812 a          281 c          281 g          826 t
ORIGIN      
        1 gatgttttac ctatattaga tcaatggcat ttagatccag aaaactggta ttctaaattt
       61 tttgaaaaat aaataaaata atcgaaattg gcattttatt tttgaaaatt tggtgaaaga
      121 ctcatcacat taagggttta aaataataag ctaagataaa tttattttat atcatttcat
      181 ttgttatcta aacaaagaaa atgacaaaaa ggaaattata tgataggaaa atgatttgat
      241 aaagaatatg tattatttgg atatttgatg aattgctact acaagtatat taacaagtcg
      301 ttttctaatt cagttttaat tggagcatag aattattttt attctcattc cattatgaca
      361 aaaaaatgta agaaaatttt ttatctaatt tctattacaa taaattttta aaataaaaag
      421 agtttaatat ttacaattca cctcttttca attttattaa taccttggtt aattcatcat
      481 aactaatggg tttagcaatg actttgcgat ctttgtctag tagaaacatt gtaggagtag
      541 cataaacatt atataatttt actacattgc tattccatcc tttaaattca gaataatgaa
      601 tccaacgatt tttaatattt ttaatatcaa taaaagtttc taacatttgt ggcactgtat
      661 ctagtgaaat agctaccgta gtaagagtag aatctgggaa atccattgta gtctcataaa
      721 tatatggtat ctcctcagtg caatgaggac accagctagc ccaaaaaacc aataatatat
      781 atttggttgg taaatcatac aaagaaatat ttttttgtgg aattacaaaa tctggtgcag
      841 ggtttcctaa ttctatacct ttgtatttat ctgccaattt tctagcccaa gcccattgat
      901 catcttgtga accacaatct ccttcttctg ttttcaaata ttcatcatat actattataa
      961 atgctttctc taagtgctga tctttaaaaa aatttaaaaa ataggataag caaaaatttc
     1021 taactgctaa atttttttct gtgtgtttga gaaaatctaa agcagcagaa gcaaataaaa
     1081 actcgtcata tactggttgg ccatactcat cacgtatatt tgttttcaat ttcatataca
     1141 tttccatttt ttcaggtaaa acaaaagtgt ataaaaagat gggatcagat ggatcgaaat
     1201 tatcaaaaaa atgtgcagcc atcacgctat ctctccatgg atcgggcatt ttccaatctg
     1261 gattaggtaa ataatatgct aaagctatgc gatgagcatt gtatttcaga ttttttttac
     1321 ttttttccat aaagttagct aaattctcat atctatcatt gtactcttgt actaaggttt
     1381 tatgaaaagg atctataata ggatataatc ttaacatttg taaaagccaa tattggctta
     1441 tcatccagct acgttgatat tttagaaatt tataaaattc tttattttct ttagatttaa
     1501 tgattttcat actatcttct gcataattcc acatttggtt aggattgtaa acagttttta
     1561 gcttaacatc attttcatct tttaaaatat aaaaaaattg tgaactattg cctaaccgca
     1621 cctcatattg tatacctgta tctggcattt tgaaattaaa atttccttta tcatcagtta
     1681 tagagctatc tattaatttt tgttcgttac catagtatga aaataagtaa agtgatatgt
     1741 tagaagcatg ttctattgtc ccagagatat tctgagcatt tacactaata aaaaatgaag
     1801 aaaataaaat aagtaaaata agttttaaag atttcattaa gtgctgcata taatttattt
     1861 ttaactaaat acaaaattaa aaattttttt atatatcttt ctattaacta ttaatttttt
     1921 taagaaattc ttaatattta aaaatagaaa aattaaatat aattttgcta aaaaataata
     1981 caaattatta tgttaggaac ttataatatt gaaagaaaat taaattttgc tacattagag
     2041 gacataatgt atcatagagt agatgtacaa ttgagtgagg tagctaaaaa taatattcaa
     2101 aaatcaagac aatttttaga taattattta gaaaaaaatg ataaccctgt ttatggcatt
     2161 aatactggat ttggagcatt atattctgtt aaaattccta
//
LOCUS       DAOPRN010000122         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_39219_length_2195_cov_98.424299, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000122 DAOPRN010000000
VERSION     DAOPRN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39219_length_2195_cov_98.424299"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..>2195)
                     /locus_tag="PKI65_08445"
     CDS             complement(264..>2195)
                     /locus_tag="PKI65_08445"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOB78497.1"
                     /translation="SKLIETIVNENIFGLDIEEFPLYLAEMNILMRLLPLIINKKYNN
                     PIDKKIKVFLTKDSVAEFFDTALRNTINDISVKSGQLSINFDELDLGYKSYVREEDDL
                     EEMKKSLEEHPHIPRRRFDYVIGNPPYVSYNECSKQSLLTFELIKKGKVQLSNIYGVN
                     LHSIPNNPKKYAPKPNLYAFFIALGLALLKDNGKLCYIIPQTILTAGDLDVIRYHLSK
                     YTTIEKIVIFNNKMFINRGVKQNKIIPTSSLIFIVSKNKLPLMSNHEVEIINYKNENH
                     SIDKTLENILHGKYIDKKKISQNILLKNVENWNFVKQNKNFLSFYESYQKNTENISIY
                     YDHVTAEQIFGNKFYFDIGFTLDYNEIVDKIENDYYEILDFKNFRNYTYFIPNKFYPN
                     DKNKIKLTQNNQGYITLEQKYKIVWSIKNPEKFYFTDHNVIFYMGKAGIICSNNYDEI
                     FYLFSILNNPINKYILTKMLKNENEKDYLVSIKAIKQYVRVPNINDRNKSIKNEIIQC
                     TEEMLELEEKKLSDFIDFSNIMIQKFDEIKLGKDKLILIKDKNKFSLDIKGNNIQKEL
                     IKKIINEKFINENVDNITLLDLKDLPIIDYDLQKQLKDYIDDLVFALYFNVHIDKLGL
                     DEAQNIKDKCKKNQFYDIL"
BASE COUNT          772 a          271 c          214 g          938 t
ORIGIN      
        1 cataacctac tgtatcattt attttaaact aattacaaag ataaaattaa atttttgaaa
       61 tacatgtttt aattaataaa aattattact ttgaatatac taataataac tacgttgctt
      121 aataatataa tttgatattt aaaaaaaaaa ttcatttcca aatgcgagta taattctaat
      181 agagattaaa tattggagct attattttag aaaaaaaata agaaaaaatt ttaatctata
      241 cttttatttt aaaaaaaaat attttataaa atatcataaa attgattttt cttacattta
      301 tcctttatat tttgggcttc atctaatcct agtttgtcta tatggacatt aaaatataat
      361 gcaaaaacta aatcatcaat ataatctttt aattgtttct ggaggtcata gtcaattata
      421 ggtaaatcct tcagatctaa aagtgtaata ttatcaacat tttcattaat aaatttttcg
      481 tttattattt ttttgattag ttccttttgt atattatttc cttttatatc taatgaaaat
      541 ttatttttat cttttattaa tattaattta tcttttccta attttatttc atcaaatttc
      601 tgaatcatta tattagaaaa atcaataaaa tcagataatt ttttttcttc taattcaagc
      661 atttcttcag tacactgtat tatttcgttt ttaatagact tatttctatc gttaatattc
      721 ggaacacgaa catattgttt gatagcttta atagacacta aataatcttt ttcattttca
      781 ttttttaaca ttttagtaag aatatattta ttaattggat tatttaaaat ggaaaataaa
      841 taaaatattt catcataatt attagaacaa attataccag ctttacccat ataaaaaata
      901 acgttgtggt cagtaaaata aaatttttct ggattcttta tactccaaac aattttatat
      961 ttttgttcaa gtgttatata cccttgatta ttttgtgtca atttaatttt atttttatca
     1021 tttggataaa atttattagg tataaaatat gtataattac gaaaattctt aaaatctaaa
     1081 atttcataat aatcattttc tatcttatct actatttcat tataatccaa tgtgaatcca
     1141 atatcaaaat aaaatttatt tccaaaaatt tgttcagcag ttacgtgatc ataataaatg
     1201 gatatatttt cagtattttt ttgatacgat tcataaaaac ttaaaaaatt cttattttgt
     1261 tttacaaaat tccaattttc cacatttttt aataaaatat tctgtgatat ctttttttta
     1321 tcaatatatt taccatgtaa aatattctct aatgtcttat caattgagtg attttcattt
     1381 ttataattta ttatctctac ctcatgattc gacataagag gaagtttatt ttttgaaaca
     1441 ataaatatta agctcgaagt aggaataatt ttattttgct ttacacctct atttataaac
     1501 attttattat tgaaaattac aattttttca atagttgtat atttggataa atgataacga
     1561 atcacatcaa gatctccagc agttaagata gtttgtggaa tgatgtaaca taatttaccg
     1621 ttatccttta ataatgccaa tcctaatgct atgaaaaatg cgtatagatt gggctttggt
     1681 gcatattttt taggattatt aggtatgcta tgtaaattaa cgccataaat gttacttagt
     1741 tgcactttac ctttttttat tagctcaaat gttaacaaac tttgttttga acattcattg
     1801 taactaacat agggtggatt gccaattaca taatcaaaac gtcgtctagg tatatgtggg
     1861 tgctcttcca agcttttttt catttcctcc aaatcgtcct cttctcgtac ataactttta
     1921 tagccgagat ctaattcgtc aaaatttatt gataattgtc cactttttac acttatatca
     1981 ttaattgtat ttcttaaagc tgtatcaaag aattcagcca cactatcttt tgttagaaat
     2041 acttttatct ttttgtcaat aggattgttg tattttttat ttataattaa tggcaaaagg
     2101 cgcattaata tattcatttc tgctagataa agtggaaatt cttctatatc aagtccaaag
     2161 atattttcat taacaatcgt ttctattaat ttaga
//
LOCUS       DAOPRN010000123         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_39655_length_2175_cov_77.751415, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000123 DAOPRN010000000
VERSION     DAOPRN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39655_length_2175_cov_77.751415"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..728)
                     /locus_tag="PKI65_08450"
     CDS             complement(144..728)
                     /locus_tag="PKI65_08450"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MjaI family restriction endonuclease"
                     /protein_id="HOB78498.1"
                     /translation="MKIKINNDDIRAYLDIEKINLDKYVSPLINLANQYAQGTSPKVV
                     GQMSELIQQFNGKTISEWKQWYLANNPDAIENATKKIWTKIQELKGTINNINESDVEK
                     WVEDLVIVKTFTGLKFQEVILKKAAEIKNTTYRLSNPDEESRGIDGFIGDIPVSIKPT
                     TYKLESALKEQIEVKIIFYQKLKDGIEVDYGEII"
     gene            complement(736..2034)
                     /locus_tag="PKI65_08455"
     CDS             complement(736..2034)
                     /locus_tag="PKI65_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOB78499.1"
                     /translation="MKTHHKIIIGDSRNMEEIADESIHLIVTSPPYWQLKDYGNENQI
                     GYNDTYEEYINNLNLVWSECHRVLHKGCRLCINIGDEFARSVYYGRYKVIPIRTEIIK
                     FCENYGFDYMGSIIWQKVTTCNTTGGATIMGSYPYPRNGIIKLDYEFILIFKKYGNAP
                     NVSKEIKELSKLTNEEWNQYFVGHWNFSGEKQDKHLAMFPEELPKRLIKMFSFVGETI
                     LDPFLGSGTTSLAAKKLNRNSIGYEINEDFLQIIKEKLDIDKKEIFNENIYEIIKQSN
                     FDNNYKERINKLPYIFNDPIKFDKKIDPKKFNFGSKIDNTENYKNKFYTVTDIISPEV
                     LILDNQLKIRLLGIKEIFEKRNEAIAFLNEKIYKQKVFIKYDNIKYDENDNLLCYLFL
                     KNGMFINNYLVKKGLADIDEKYNFIYKTKFQNLKNKISIK"
BASE COUNT          729 a          300 c          209 g          937 t
ORIGIN      
        1 attttgaaat ggctaggaaa tatttagaag aaattataga aaatactgtt gaaacacttg
       61 ctaaagtgcc tcagcttgag tgatttaata tttgtagttc aaaaataata tattattttt
      121 ttattaaaga gaaataataa ttatcaaatt atttcaccat aatctacttc aataccatct
      181 ttcaattttt gataaaaaat tattttaact tctatttgct cttttaatgc tgattctaat
      241 ttataagtag taggttttat tgatactgga atatcaccaa taaatccatc tattccccgt
      301 gattcttcat caggattcga taatctatag gtagtgtttt taatttcagc agcttttttt
      361 aaaataactt cttgaaattt aagtcctgta aaagttttaa caataacaag atcttctacc
      421 catttttcta cgtctgattc attaatatta ttaattgtac cttttaattc ttgtattttt
      481 gtccaaattt ttttagttgc attttctata gcatctggat tattagctaa ataccattgc
      541 ttccattctg aaattgtttt accattaaat tgttgtatta attcactcat ttgccctact
      601 actttaggac tagtaccttg tgcatattga ttagctaaat taattaaagg cgaaacatat
      661 ttatctaaat taattttttc tatatcaaga tatgctctaa tatcatcatt attaatttta
      721 attttcataa aaatattatt ttatagaaat tttattttta agattttgaa atttagtctt
      781 atatatgaaa ttatattttt catcaatatc agctaatcct ttttttacaa gataattatt
      841 tataaacatg ccatttttta aaaataaata acaaagtaga ttatcatttt catcatattt
      901 aatattatca tatttaataa atactttttg tttataaatt ttttcattta aaaaagcaat
      961 agcttcattt cttttttcaa agatttcttt aattcctaat agtctgattt ttaattgatt
     1021 atctaaaatc aaaacttcag gtgaaataat atctgtaact gtataaaatt tatttttata
     1081 attttcagta ttatcaattt tagatccaaa attaaatttc ttagggtcaa tttttttatc
     1141 aaattttatt gggtcattaa aaatataagg caatttattt attctttctt tataattgtt
     1201 gtcaaaattg ctttgtttaa ttatttcata aatattttca ttaaatattt cttttttgtc
     1261 aatatcaagt ttttctttta taatttgaag aaaatcttca tttatttcat atcctataga
     1321 gttcctattt aatttttttg cagctaatga tgttgttcca cttcctaaaa aaggatctaa
     1381 aatagtttca ccaacaaaac taaacatttt aattaatctt tttggcaatt cttctgggaa
     1441 cattgccaag tgtttgtctt gtttctcacc tgaaaaattc caatgaccaa caaaatattg
     1501 attccattct tcattagtta attttgataa ttcttttatc tctttactaa cattaggagc
     1561 atttccatat tttttaaaaa tcaaaataaa ttcataatct aattttatta taccatttct
     1621 aggataagga taagatccca ttattgtggc tccacctgtt gtgttgcatg tagttacttt
     1681 ttgccaaata atggagccca tataatcaaa accataattt tcacaaaatt ttataatttc
     1741 cgttctaatg ggtattactt tatatctgcc ataataaact gaacgtgcaa attcatctcc
     1801 aatatttatg cataaacgac atcctttgtg aagaactcta tggcattcac tccataccaa
     1861 atttaaatta tttatatatt cctcatatgt atcattgtat cctatttgat tttcattgcc
     1921 ataatctttt aattgccaat atggtggaga tgtaactatt agatgaatag attcgtctgc
     1981 tatttcttcc atatttctac tatcaccaat aattatttta tgatgtgttt tcatagagct
     2041 aatattttta atatatttat atttagcaaa attagtattt tattttttaa aattcaatta
     2101 ataattttga aaccttataa aaagtgcctc agcttgatta attataaaaa ttccattgca
     2161 gaccccatct aaatg
//
LOCUS       DAOPRN010000124         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_39701_length_2173_cov_93.440982, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000124 DAOPRN010000000
VERSION     DAOPRN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39701_length_2173_cov_93.440982"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKI65_08460"
     CDS             complement(<1..196)
                     /locus_tag="PKI65_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78500.1"
                     /translation="MTQLKKDEILYERAKKVLPGGASRNQVLRQPHPVYAASASGSYL
                     TDVNGKQYIDFSANIGSLIHG"
     gene            211..357
                     /locus_tag="PKI65_08465"
     CDS             211..357
                     /locus_tag="PKI65_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78501.1"
                     /translation="MLWCKIKYFFGFIIVKFHKVVYGFSYELNVKTPFDAIEKPVVIK
                     AEPW"
     gene            555..1412
                     /locus_tag="PKI65_08470"
     CDS             555..1412
                     /locus_tag="PKI65_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78502.1"
                     /translation="MINTGKEIIMETKLIEDLKSRYKLFMEDLDIRENGLLGKISNPR
                     YKDQKLRFSGFPYVGSKYSVAKKKILFVGQDIGKDECREDDSFHDLDSRRISIAGSVN
                     GCISLDYNDHISGTYVMALFLLREYYSWEEAWNKLSSMNDITSKTAINRFKSILPIDV
                     LDYVALTNIYKFVSLCRGCDPNNNKPKCWNQKCIEQNKIKNRSGSNNRKWYNKQEEID
                     MFLDEIHIFKPDLVYFQGSSAKLNSSILNKINSFCDICISYHPSAWNVGANKPNFANK
                     IKIKPKTSN"
BASE COUNT          806 a          283 c          335 g          749 t
ORIGIN      
        1 gaccatgaat taatgaaccg atattagctg agaaatctat atattgcttc ccattaacat
       61 ctgttaaata tgagccagaa gcactagctg catatactgg atgtggctga cgtaatactt
      121 ggtttctact cgctccacca ggtaatactt tcttagctct ttcatataag atttcatctt
      181 tttttaattg tgtcatattt ttaaaatttt ttgttatggt gcaaaattaa atattttttt
      241 ggttttataa ttgtaaagtt tcacaaggtt gtttatggct ttagctatga gcttaatgtg
      301 aaaacacctt ttgatgctat tgaaaaacct gtggtgatca aagctgaacc atggtaagaa
      361 attaatccga aaatttttaa agaaaatcca ttgcagttta aaaataaaat tgtatatttg
      421 caacaaaata atattcatag cacacggttt aaaccgtgtg ctatgaatat taacgaataa
      481 caaaacttga cacggggatg ctaaaaaccg aacagattag gcattcaaat ttaaaaccat
      541 aaattatgga gcaaatgatt aacacgggca aagaaataat catggaaaca aaactaattg
      601 aagatctaaa atccaggtac aaattattca tggaagatct agatatcaga gaaaatggac
      661 ttttaggaaa aatctcaaat ccgcgatata aagaccaaaa attaagattc tctgggtttc
      721 cttatgttgg gagtaagtat tcagttgcaa aaaagaaaat tctttttgta ggtcaagata
      781 taggaaaaga tgaatgtaga gaagatgatt cttttcatga ccttgactcg agaaggataa
      841 gtattgcagg aagtgttaat ggttgtatat cattagatta caatgatcat atttcaggaa
      901 cttatgtgat ggccttattt ttattgaggg aatattattc ttgggaggag gcttggaata
      961 aattatcaag tatgaatgat ataacttcta aaacggcaat aaataggttc aaaagtatat
     1021 tgcctattga tgtactagat tatgtcgctt taactaatat ttataagttt gtctcgttat
     1081 gcagaggatg tgatccaaac aataataaac ctaagtgttg gaatcaaaaa tgtatagaac
     1141 aaaataagat aaaaaatcgg tcaggaagca ataatagaaa gtggtacaac aaacaagagg
     1201 aaatagatat gtttctggat gagatacata tatttaaacc agatctagtt tactttcaag
     1261 gttcttcggc caaattaaac tcttctattc tcaataaaat taattcattt tgtgacattt
     1321 gtatttcata tcatccttcc gcttggaatg tcggtgccaa taagccaaac tttgctaata
     1381 aaattaaaat caaacctaaa acttctaatt aatactcaat cattacttat tatggaatca
     1441 taaaatacaa cacactggat actataaata gtttggatgg agtgtttgtt gatagaaaag
     1501 ttattacctg tattagccct tgatatccga gcactttaat tgcatttaat tttatatatt
     1561 attttttgac tcggacaaac ataacctcag tatctttatt acccgatggt atattttttt
     1621 ttgtgagttc aaactccatt tacatttgta cgatcttacc ctaacttgga aatcatagtc
     1681 taaaaaacca aatttataat tatttaactt tttgtagaat actgaatatc tggcatataa
     1741 aaacataaca aattttaaca tgacatggta taatcataaa acattgaata taaagtagtt
     1801 agtcttttat gtttttttgg tagtccccat aggtttgttt gctaaaaata ttctacctat
     1861 cttttcagca tgtttgtaaa gaaaattata aagcagaata aaaatttaaa taagtaatat
     1921 acatattatc gcttagtaca tgtaaatttt taaagaaaat acattgcaga tttaaaaata
     1981 aatttttttt gtaacaaaat aaaggttata taacgtttat tttgggtaaa ttagttaggt
     2041 taatatttaa ctctctaata ataaataaaa ttacagaagc tcagaaatta attgaatagt
     2101 gaaaatattg attaaaaaat taagtcagct tcagttttat ttattagagt atttttatca
     2161 atgcctggag cca
//
LOCUS       DAOPRN010000125         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_41343_length_2106_cov_196.145783, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000125 DAOPRN010000000
VERSION     DAOPRN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41343_length_2106_cov_196.145783"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1105
                     /locus_tag="PKI65_08475"
     CDS             107..1105
                     /locus_tag="PKI65_08475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOB78503.1"
                     /translation="MKISILIPTKNRLPILRETLHNIYYVNKLTREDYEVIVSNDGDD
                     DLSVIYDEFPFDNLKIVKNNHKAGLSGNRNNAIEFSQYEILQFLDDDILITEDFLIKV
                     LTVHKKFDNIILCGNRYYPQSLIEIAEKTPFGRYKLKYEYNWLDGLMMNEIDDGLYEV
                     NYLAGFSISMKREIFDEVGKFDENFDYAGCEDAEFFYRASQKNVKLLFDEKNICYHNE
                     LDNFTLDAWLRRQSTGIRSAVVVCKLHPEGQNHPTWYTNTSIKASDNWNVKLLKLKKI
                     FCSFPPVYDFIHFLAIYGEKIKLPDSFLFRLYNALWLGATYRGFREAYKEIFINKK"
     gene            1110..1955
                     /locus_tag="PKI65_08480"
     CDS             1110..1955
                     /locus_tag="PKI65_08480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOB78504.1"
                     /translation="MISIIILNYNTFDLTCQCIESIYKETKKVDFEIIIVDNASTVDD
                     PDKFLELFPKIKLVKNTENRGFAGGCNDGIKVAKGDTILLLNSDTKLLNDAISITYDF
                     LNTHPNVGIVTCRLENEDGSPQNNCQAFPKIKNILIELFRIHKLLPHKRVAKILYGPY
                     SDYSTIAYPHWVWGTFFMFSKKLLNIFPNQLLPETFWMYVEDMQWCWLARQAGYEIAF
                     VPNGIVLHYGGKNHNPKAIKMMNDNFNQFLKLYYNKMHAWIIIRLLKILEWSQWRKWF
                     AKNKE"
BASE COUNT          802 a          243 c          335 g          726 t
ORIGIN      
        1 attgtattgc ttagctataa aattacatcc attttggcga ggcaattata ttgattttgt
       61 taaatatatg tttaaaaaat aattttttaa atttgtttta taaaaaatga aaataagcat
      121 actaattcct acaaaaaatc gactacctat actgcgagag actttgcata atatttatta
      181 tgtaaataaa ttgacaagag aggattatga agtgatagtg agtaatgatg gcgatgatga
      241 tttgtcagta atatatgatg aatttccttt tgataatctg aaaatagtaa aaaataatca
      301 taaagcagga ttgtctggta acagaaataa tgctatagaa ttttctcaat acgaaatatt
      361 gcaatttcta gatgatgata ttctgataac tgaagatttt ttaattaaag tacttactgt
      421 tcataaaaaa ttcgataata tcattttatg tggtaataga tactatccgc agtctttaat
      481 tgaaatagct gaaaaaactc cttttggcag atataaacta aaatacgaat ataattggtt
      541 agatggatta atgatgaatg aaatagatga tggattatat gaagtaaatt atttggctgg
      601 tttttctata tcaatgaaaa gagaaatatt tgatgaagta gggaaatttg atgaaaactt
      661 tgattatgca ggttgtgagg atgcagaatt cttttataga gcatctcaaa aaaacgtaaa
      721 actactattt gacgaaaaaa atatttgcta tcataatgaa ctcgataatt tcacattaga
      781 tgcatggctt agacgtcaaa gcactggtat tcgtagtgct gttgtagttt gcaagctaca
      841 tcctgaaggg caaaatcatc ccacttggta tactaatact tctataaaag cttctgataa
      901 ttggaatgtt aaattattaa aactcaaaaa aatcttctgt agttttcctc cagtttacga
      961 ttttatacat tttttagcaa tttatggtga aaaaataaaa ttgcctgatt catttttgtt
     1021 tagattgtat aatgctctat ggttaggagc tacatatcgt ggatttagag aagcttataa
     1081 agaaatattt ataaataaaa aataaaacca tgatttccat cataatactc aattacaata
     1141 cttttgatct tacttgtcaa tgtattgaat ctatttataa agaaacaaaa aaagtagatt
     1201 ttgaaattat catagtagat aatgcatcta ctgtggatga tccagataaa tttttggaat
     1261 tatttcctaa aataaaatta gtaaaaaata ctgaaaatcg tggcttcgca ggaggatgta
     1321 atgatggtat aaaagtagct aaaggtgata ctatattact tctaaactct gatactaaac
     1381 tgctaaatga tgctatttct atcacttatg attttttgaa cacacatcca aatgttggta
     1441 tcgtaacgtg cagactagaa aatgaagatg gctctccaca aaataattgc caagctttcc
     1501 caaaaatcaa aaatatttta attgaattgt ttagaataca taagcttttg ccacataaga
     1561 gagttgctaa gatattatat ggtccatatt cagattattc tactatagca tatcctcatt
     1621 gggtatgggg tacatttttt atgttttcaa aaaaattatt aaatattttc ccaaatcaat
     1681 tattaccaga aaccttttgg atgtatgtag aggacatgca atggtgctgg ctagctagac
     1741 aagctggcta tgaaatagca tttgtaccta atgggatagt tttacattat ggtggcaaaa
     1801 atcacaatcc caaagctata aaaatgatga atgataattt taatcaattt cttaaattat
     1861 attataataa aatgcatgca tggattatta tcagattatt aaaaatatta gaatggtcac
     1921 agtggagaaa atggtttgca aaaaataaag agtaataggg tttagaaatt aaatttggtt
     1981 ctataacgtt ttataagggt atagattaaa aaagagtaat tttatataaa tgaatttttt
     2041 taatattttt gggttaaata ttaaccacaa agttctcaaa gaaggcacaa agaacacaga
     2101 gaatga
//
LOCUS       DAOPRN010000126         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_42366_length_2067_cov_257.785288, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000126 DAOPRN010000000
VERSION     DAOPRN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42366_length_2067_cov_257.785288"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PKI65_08485"
     CDS             <1..796
                     /locus_tag="PKI65_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOB78505.1"
                     /translation="KDNLTLFTTDAKTSRNSAKVQVSAAEVLIKEAEKTNNPVKKEKL
                     LLEALQYLKRGGEIYPEYYGVYDLGGKAQFYFKNYDLSLENYLICTKLNPKIPTPYNN
                     IYAVGVNAFNDSVYDVAHRAFSFLTNKYPDSTKYLFQLALVYKSTYKIDSAIIILNKI
                     IKKDSCNADAYNQLGEIYARFLGNNRKAEEYLLKAVQCDPTHSSALENLGVFYGMQGR
                     SKDALNYFKKAHKYNPNSKNLIQNIGLAYKNLGMMDSAQYYLQKAM"
     gene            1347..>2067
                     /locus_tag="PKI65_08490"
     CDS             1347..>2067
                     /locus_tag="PKI65_08490"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78506.1"
                     /translation="MKKIVENTSKYLAMSKEENKEFLDNLYSLMKKLEADVVDYQGLK
                     ILSAPPLCPDCRSNHIIKNGLQNGMQNYRCKDCGRQFRVTTGTFVYRLQKKEKMLDYI
                     RCMVAGKSLRACAKEVGISLPTSFAWRHKILAALQSFEDNINFFGVVEMDELLMDYSE
                     KGRIYKNAEELKRARKQKPKKVAVLAATDRAGNLLFKKFDGKKLSSEQIEQFLKAKMP
                     KDGVMCSSNKKAFKKVGRNVIK"
BASE COUNT          804 a          282 c          364 g          617 t
ORIGIN      
        1 gaaagacaat ttaaccttat tcactactga tgctaagacc tcgcgtaata gtgcaaaagt
       61 gcaagtatca gctgctgaag tcttaataaa agaagcagaa aaaacaaata atccagtgaa
      121 aaaagaaaaa ttgttgttgg aagcattaca atatttaaaa aggggaggtg agatatatcc
      181 tgaatattat ggagtttatg atttaggggg caaggctcaa ttttatttta aaaattatga
      241 tttatcatta gaaaattatt taatatgtac taagttaaat ccgaaaattc ctactcccta
      301 taataatatt tatgcagtag gagtaaatgc ttttaatgat tcagtatatg atgtagcaca
      361 tcgtgctttt tcatttctca ctaataaata tcctgattca actaaatatt tgtttcaatt
      421 ggcacttgtg tataaatcta cctataaaat agattctgct ataattattt taaataaaat
      481 aataaaaaaa gatagttgta atgcagatgc ttacaatcaa ctaggagaaa tttatgctag
      541 atttttaggt aataatagaa aggcagagga atatttatta aaagcagtgc aatgtgatcc
      601 tactcattca tcggcattag agaatctagg agtattctat ggtatgcaag gtagatctaa
      661 agatgctcta aattatttca aaaaagcaca taaatataat cctaatagca aaaatcttat
      721 acagaatata ggtttagcgt ataaaaatct agggatgatg gacagtgccc aatattatct
      781 acaaaaagct atgtaataaa tatttattgg attattcgtt tggttgtctc atcttgaaat
      841 ggatttatat aaataaatca ttgtaagggc gtataatcat attcccataa tcatgcaccc
      901 tatttatcct ggcctttagg tcattagtaa aacaaagaat aagacgtgac attagctcta
      961 aaaacatgat tatcgccacc ttcgatacaa ccataaatgg actacttagc caccaaacga
     1021 ataaaatttt agtttgatat aaaaatggat aatttcgaaa aaattagaat aagtatgcct
     1081 tttgctgaac tcatgattat gtttgcaata acgtagatta aggagccaaa aaactaacaa
     1141 aactaaaata atttggatta tttatattat aatattattg tgaaaaaaaa gcagtaaatt
     1201 ttgtgtttgt ataggtaatg aaaattggga agtttggcaa ggggatggaa tgaataaatg
     1261 atataaaatt attacattaa taaatgttta agtttttcaa gtttatatgt taaataattt
     1321 ttatattttt acaaaaattt taattcatga aaaaaatagt agaaaacacc agtaaatacc
     1381 ttgctatgag caaggaagag aataaagaat tcttagacaa cctttattct ctaatgaaaa
     1441 aattagaagc cgatgtagtc gattatcaag gtctgaaaat actcagtgcc ccgccactct
     1501 gtccagactg tcgtagcaat cacatcatta aaaatggctt acaaaatgga atgcaaaact
     1561 atcgttgcaa agactgcggc agacaattta gagttaccac tggtactttt gtctacaggt
     1621 tacaaaagaa agaaaaaatg ctcgactaca tcagatgtat ggtagcaggc aaaagtctca
     1681 gagcttgtgc taaagaggtg ggcatcagcc taccaacaag ctttgcttgg cgacacaaga
     1741 tattagcagc tttacaaagt ttcgaagata atatcaattt tttcggcgta gtagagatgg
     1801 acgaactatt gatggattat tccgaaaaag gcagaatata caaaaatgcc gaagagctga
     1861 agcgagctag gaagcaaaag ccaaagaaag tagccgtatt ggcagctact gacagagctg
     1921 gcaacttatt attcaaaaaa tttgatggca aaaagctaag ttcagaacag atagagcaat
     1981 ttctgaaggc taagatgcca aaggatggag ttatgtgtag cagtaataaa aaagcattca
     2041 aaaaggttgg aagaaatgta atcaagc
//
LOCUS       DAOPRN010000127         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_42804_length_2051_cov_112.848196, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000127 DAOPRN010000000
VERSION     DAOPRN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42804_length_2051_cov_112.848196"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1326)
                     /gene="serS"
                     /locus_tag="PKI65_08495"
     CDS             complement(40..1326)
                     /gene="serS"
                     /locus_tag="PKI65_08495"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOB78507.1"
                     /translation="MIPISTIRTETERVKKGLKKKNFPNLELIDEIIQLDNQKRQQQT
                     NRDELQKQLNDLSRQIGTLIKEKKNIEAEKLKNDTFFLKQEIQEKESILKDLENRINE
                     ILILLPNVPHESVPDGYSSQDNIIVKSGGELPKLDCKAKPHWELAEDYDLIDFALGAK
                     ITGTGFPVYKSWGAKLERALIQFFLDKAWDHGYVEIWPPILVNEASAFATGQLPDKDG
                     QMYYINLDELYIIPTAEVPITNIYRDTIIAEDDLPIKNVAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKIEIVQIAHPDKSYQILEEMVCYVESLVQALELPYRIVKLCGGDLSFTSA
                     LTYDFEVFSAAQKRWLEVSSVSNFENFQANRMKLRIKTNMGKTILAHTLNGSALALPR
                     ILAALLENNQTPNGIKIPKILVKYMGIEMINKFGSI"
     gene            complement(1447..>2051)
                     /locus_tag="PKI65_08500"
     CDS             complement(1447..>2051)
                     /locus_tag="PKI65_08500"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOB78508.1"
                     /translation="VENIKNVCIDMSPAYISGVYYNFPNANIVFDKFHVKKLLNEAFY
                     KVWQLERRENEMLKGHKYTFLKDYRKLTEDKKAQLNMLTLMYPKLGEAYRLKVMFDEF
                     WQIKDMQEAQGYLAFWCDMVEDSKIHPLMEFAKTIKAHWTGIVNYIKAKITTGVIEGI
                     NNKIQLAKRRARGYRNINNFINMIYFLCGKLKFDYPYYPL"
BASE COUNT          610 a          341 c          292 g          808 t
ORIGIN      
        1 aaatattgga acatattttt gcaaatagtg gcagatttat taaatggagc caaatttgtt
       61 aatcatctct attcccatat attttacaag gattttaggt attttaatcc catttggtgt
      121 ttggttattt tctaataaag ctgcgagtat gcgaggtaga gctagagcac taccattgag
      181 agtatgagct agaatagttt tgcccatatt ggtttttatt cttaatttca ttctattagc
      241 ttggaaattt tcaaaattgg atacagaact aacttctaac catcgtttct gtgcagcaga
      301 aaatacttca aaatcataag taagcgcaga ggtgaagcta agatctccgc cacataattt
      361 gactatacga tatggcaact ctaaagcttg tacaagtgat tctacatagc ataccatttc
      421 ttcgagtatt tgataagatt tgtcagggtg ggctatctgt actatttcta ttttatcgaa
      481 ttgatgtagc ctattcaagc ctctcacatc tttgccataa gatccagctt cgcgacgaaa
      541 acatggcgta taagcaacat tttttatagg taaatcatcc tcggctataa tagtatcgcg
      601 ataaatatta gtgataggta cttcagcagt tggtattatg tatagctcat cgagattaat
      661 ataatacatt tgtccatctt tatcaggtaa ctgcccagta gcaaatgctg atgcttcatt
      721 tactaaaatt ggaggccata tttctacgta tccatggtcc catgctttat ctaaaaaaaa
      781 ttgtataagg gcacgctcga gttttgctcc ccaactttta taaacaggaa aacctgttcc
      841 agttatttta gcacctaatg caaaatctat caaatcatag tcctctgcga gctcccaatg
      901 tggcttagct ttgcaatcta gttttggtaa ctcaccacca ctttttacga ttatattatc
      961 ttgtgatgag tatccatctg gtacggattc atgaggcaca ttaggtaaaa ggattaatat
     1021 ttcatttatt ctattttcta aatctttaag aattgattct ttttcttgaa tttcttgttt
     1081 taaaaaaaaa gtatcatttt ttagtttttc tgcttcgata ttcttttttt cttttatcaa
     1141 tgtacctatc tggcgagaca aatcatttaa ttgtttttga agttcatctc tattggtttg
     1201 ttgttgcctt ttttgattat ctaactgaat gatttcatct atcaattcta aatttgggaa
     1261 attttttttc tttaaacctt ttttgaccct ctctgtttca gtgcgtatag tagatatcgg
     1321 tatcatattg caaattttat tgcaaattta atggatattt tataattaat tatatttaat
     1381 acaaaatgtt tctatttcta aatcaggtgc ttatattttt aaaaataaac atatattact
     1441 tgggttctat aacggatagt atgggtaatc aaattttagc ttaccacata agaaataaat
     1501 catattgata aaattgttaa tatttctata gcctctagct cttcttttag caagttgaat
     1561 tttattgttt attccttcta taacaccagt agtaattttt gctttaatgt aattaacaat
     1621 gccagtccaa tgtgctttta tagttttagc aaattccatt aggggatgta tcttggagtc
     1681 ttctaccata tcacaccaaa atgccaaata tccttgtgct tcttgcatat cttttatttg
     1741 ccagaattca tcaaacataa cttttaacct atatgcttct cctaattttg gatacattaa
     1801 tgtgagcata tttaattgtg ctttcttatc ctctgttagt tttctgtaat cttttaaaaa
     1861 tgtatactta tgacctttca gcatttcatt ttcccttcgc tccaattgcc atactttata
     1921 aaaggcttca tttaataact tttttacgtg aaatttatca aagacaatat tcgcatttgg
     1981 aaaattataa tatactccac ttatatatgc tggtgacata tctatacata catttttaat
     2041 attttctact t
//
LOCUS       DAOPRN010000128         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_43033_length_2043_cov_178.883300, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000128 DAOPRN010000000
VERSION     DAOPRN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43033_length_2043_cov_178.883300"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..1939
                     /locus_tag="PKI65_08505"
     CDS             299..1939
                     /locus_tag="PKI65_08505"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HOB78509.1"
                     /translation="MKKVVIIGGVAGGASTAARLRRLDENVEIKIFERGKYISYANCG
                     LPYYVGNIIKERNSLLVQTTQKMKKRFNLDIYTLHEVIKIDRANKSLLIKNLKTNEIF
                     QETYDILVLSSGAEPIKPPIKGIDTAKNVFNLRDIPDADKIKNFIETKNPKKAIVIGG
                     GFIGIEIADNLFARGLNVTLVEKADQILVQLDKEMATILHKHIIDKGIELILSDSVEE
                     FKNNGTTLVLNSGKQIDSDLTILSIGVKPESNLAKEAGLDIGITGGVKVNEYLQTSDE
                     SIYAIGDVIEVRDFITGLPTLKPLAGPANKQGRIVANNICGKNEKYLGTIGTSVVKVF
                     DMTAASIGNNEKILKKSGISYETIHITPPSHATYYPGATTIFMKLIFDKNNGKIYGAQ
                     AVGYDGVDKRIDVLSTAIMGGMTVSDLQYLELAYAPPFSSAKDPVNMLGYVATNVIDG
                     DLEIIHCDEFINLKKDNAVIVDVCEPIEREKGYIDCSINIPLNDLRNRLDELPKDKMI
                     YVYCQQGLRSYIAYRILKQNGFNVKSIDGGYRFYLNYI"
BASE COUNT          796 a          228 c          332 g          687 t
ORIGIN      
        1 catttttata agttatttcg ttcaaaatta gaaaaataat taaatatatc tatgtgtaaa
       61 attaaatata aattaagcaa aaaaacagat tacaaataaa ttttattttt acaaaaatac
      121 ataattttat atcaattata ttgcctttta ttcctattat ttggctgaaa tatagtctta
      181 tgataatttt tttattaagt tgattagttt aatgttttta aaatctaact attactatta
      241 tttatcattt gtaaagatta ttatagatac ttaatataat tttgtaaaaa tcaatattat
      301 gaaaaaagtt gtaattatag gtggagtagc aggtggagcc tctacggcag ctagattgag
      361 aagattagat gaaaatgtag aaattaaaat tttcgaacgt ggtaaatata tttcgtatgc
      421 taattgcgga ttaccatatt atgtaggaaa tattattaaa gaaaggaatt cattgcttgt
      481 gcagacaact caaaaaatga aaaaaagatt taatcttgat atttatactt tgcatgaagt
      541 cattaaaata gatagagcaa ataaatcatt attaattaaa aatttaaaaa ctaatgaaat
      601 ttttcaagaa acttatgata ttttagtttt atctagtgga gccgaaccta ttaagccgcc
      661 aataaaagga atagacactg ctaagaatgt ttttaacctt cgtgatattc ctgatgcaga
      721 taaaataaaa aatttcatag aaactaaaaa tcctaaaaaa gctatagtca ttggtggtgg
      781 atttattggc atagagatag cagataattt gtttgcaaga ggtctaaacg ttacacttgt
      841 agaaaaggcc gaccagattt tagtccaatt agataaagaa atggctacta ttttacataa
      901 acatataata gacaaaggaa tagaattaat cttatctgat agtgtagaag aatttaaaaa
      961 taatggaact acattagtgc ttaatagcgg caaacaaata gattcagatc ttacaatttt
     1021 atctattggc gttaaaccag aaagtaattt agctaaggag gctggattag acataggtat
     1081 aacaggagga gtaaaagtaa atgaatattt acaaacttca gatgaaagca tttatgctat
     1141 cggtgatgtg atagaagtga gagatttcat aactggattg cctacattaa agccattagc
     1201 tggtccggca aataaacaag gcagaatagt agctaataat atttgtggga aaaatgaaaa
     1261 atacttaggt actataggca cctcagtagt taaggtcttt gatatgacag cagcctcaat
     1321 aggtaataat gaaaagatat taaaaaaatc aggtatttct tatgaaacta tacatataac
     1381 cccaccttct catgctactt attatccagg agcaaccact atttttatga aattaatttt
     1441 tgataaaaat aatggtaaaa tatatggtgc tcaagccgta ggatatgatg gagtagacaa
     1501 aagaatagat gtgctatcga ctgctattat gggaggtatg actgtgagtg atttgcaata
     1561 tttggaatta gcttatgcac cgcctttctc atcagctaaa gatcctgtaa atatgcttgg
     1621 ttatgtagct actaatgtga tagatggtga tttagaaata attcattgtg atgaatttat
     1681 taatttgaaa aaggataatg cagttatagt agatgtatgt gaacctatag aaagagaaaa
     1741 aggctacata gattgctcta taaatatccc tcttaatgat ttaagaaata gattagatga
     1801 gctacctaaa gataaaatga tttatgtcta ttgccagcaa ggcttgagat cttatatagc
     1861 ttatagaatt ttgaaacaaa atggttttaa cgttaaaagt attgatggtg gatataggtt
     1921 ttatctaaat tacatttaat gaaatttttc aaatttttta aaacttttat tttgttgtta
     1981 aatattttga cagttaaata taaacctaaa taaattaccc acaataaacg ttataaaacc
     2041 tta
//
LOCUS       DAOPRN010000129         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_43922_length_2013_cov_147.388662, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000129 DAOPRN010000000
VERSION     DAOPRN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43922_length_2013_cov_147.388662"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="PKI65_08510"
     CDS             <1..1210
                     /locus_tag="PKI65_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78510.1"
                     /translation="ANTDYYGYGGYFKGGYKGVYGEVSPTGNNTYYGVYGKVSGGTGT
                     NYGVYGEASGGATNWAGYFSGNAKATGYLVVGSANTSSTTKYEMKEFFITSSHSVQNG
                     YIDISAGTLSLPAGASSLTINKIVYSVNGYHEDGNEGQHYVCVVVGGTAMGCIYETAE
                     DGYVPIDWTNTASASQTITSSQTVYLRMGDASESWPATDNYFYILNAKITLYYSYSNS
                     LQTGDVAASGRIYCNSVYSTQSVGDLAEHFNVIAPDNLQIEPGMIVTYKIGSDNVFEL
                     TDKPYSQFIAGVISENPSVLLNDASQGYPVALAGRVKVKVKPSNELIKSGDFLTTSDV
                     VGKAQKATQPGPVIGYAISDQKPGEDFVDVLLQIGRFYSGMEETNRLEVKSNENVIPC
                     KRTGFSSVKD"
     gene            1294..1803
                     /locus_tag="PKI65_08515"
     CDS             1294..1803
                     /locus_tag="PKI65_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78511.1"
                     /translation="MKKLFLLNVILFLSIFLNGQNSINDDAKARIGTYLKINPQILNV
                     QVHSIEAKSIVDIIVYSYENFLVIPDLPLNNITDAKEKSEINQLIKRLENLTTNPPSW
                     QQILTLQIEKKSTPPTPPVYAGDDKASQYYNTKSSNFNKKSSHAKANLIEMEISKSEP
                     TPLPDKTKE"
BASE COUNT          731 a          290 c          338 g          654 t
ORIGIN      
        1 tgcaaacact gattactatg gctatggggg atattttaaa ggtggatata aaggtgttta
       61 cggagaagta tcccctactg gcaataatac ttattatggt gtatatggta aagtttctgg
      121 tggtacggga actaattatg gtgtttatgg tgaagcttct ggtggtgcta ccaactgggc
      181 tggatatttc agtggaaatg caaaagccac agggtatctt gtcgtaggct ctgctaacac
      241 ctcttctact actaaatacg aaatgaaaga attttttatt acatcatctc atagtgttca
      301 aaatggttat attgatataa gtgcaggcac attatcacta ccagcaggag ctagcagtct
      361 cacaataaat aaaatagtat attctgtgaa tggttatcac gaagatggta atgaggggca
      421 gcattatgta tgtgtggtgg taggtggaac agcaatgggc tgtatatatg aaacagctga
      481 agacggttat gtacctattg actggactaa tactgctagc gcaagccaaa ctattacttc
      541 ttcacagaca gtttatttac gaatggggga tgctagtgaa agttggccag caaccgataa
      601 ttatttttat atattaaatg ctaaaataac tctttattac tcttatagta attccttaca
      661 aactggtgat gtagcagcca gtggtagaat ttactgcaat agtgtttatt ctacacaaag
      721 cgttggcgat ctagcggaac atttcaatgt tatagcacca gacaatttac aaattgaacc
      781 aggaatgata gtaacatata aaataggttc tgataatgta tttgaattga cggataaacc
      841 ttattctcaa tttattgctg gagttatttc tgaaaatccg tctgttttac taaatgatgc
      901 ttctcaaggt tatccggttg ctcttgcagg tagagtgaaa gtaaaagtta aaccttcaaa
      961 tgagctaata aaaagtggcg atttcctaac tacatcagat gttgttggta aagcccaaaa
     1021 agctacacaa ccaggacctg taattggcta tgccattagt gatcaaaagc caggtgaaga
     1081 ttttgtagat gttttactgc aaatagggag gttttattct ggtatggaag aaacaaatag
     1141 attagaagtt aaatccaatg aaaatgtaat accatgtaaa cgcacaggat ttagttctgt
     1201 aaaagattaa aatttatatc aacactagta ttgagtgtat taggacaaaa aacataattt
     1261 ccaattaatt aagaaaatat aaaaatatca aatatgaaaa agttattttt attaaatgta
     1321 attttatttt tgagcatttt tttaaatggg caaaattcaa tcaatgacga tgctaaagct
     1381 agaatcggca cttatttaaa aattaatccc caaatattaa atgtccaagt gcattcaata
     1441 gaagctaaat ctattgttga tattatagtt tactcttatg aaaattttct tgtaattcca
     1501 gacctacctc taaacaatat cactgatgct aaggaaaaat cagaaataaa tcaattaatc
     1561 aaaagattag aaaacttgac aactaatcct ccaagctggc aacaaatact aacattacaa
     1621 atagagaaaa aatctacacc tcctactcca ccagtatatg ctggtgatga taaagcttct
     1681 cagtattata ataccaagtc tagcaatttt aataaaaaat cttctcatgc taaagctaat
     1741 ctgatagaaa tggaaatttc taaaagtgaa ccaactccat tacctgataa aacaaaggaa
     1801 taaattttta ataaccttat tcttttgttt tgccttgatt taatttaaaa aaagatattt
     1861 agtttcataa aataacaatt agattaaaat acttaagtat tttacttttt gttaattttc
     1921 caataatatt tttaaaattt caatttaata aaagatttgt aataaaattt ttttgttaga
     1981 aaaaaaatta ttacttttgc aaaccaaaaa tgt
//
LOCUS       DAOPRN010000130         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_44161_length_2005_cov_180.434359, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000130 DAOPRN010000000
VERSION     DAOPRN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44161_length_2005_cov_180.434359"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKI65_08520"
     CDS             <1..278
                     /locus_tag="PKI65_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78512.1"
                     /translation="IAVLLPGKLLMIFSPDMVPLQIMETNFYAIKFINNYLLIIENQR
                     ILELLRLKDFMPVYVTSISESTKSVECNKNIVTTLTDNKIYIWEYKN"
     gene            complement(565..1557)
                     /locus_tag="PKI65_08525"
     CDS             complement(565..1557)
                     /locus_tag="PKI65_08525"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOB78513.1"
                     /translation="MEKLLKNSKEYLALPKDHKRDFLDQLYQFLIDDHATVVDYQKLK
                     IKSTAAICPNCKSENVIKAGTRKGRQIYRCKSCRYQFSETARTFVYRMRKYPLMLDYL
                     RCMLEGKSLRACADEVGICLKTSFRWRHRILAALRSFEGGISFSGIVEADELLMQYSE
                     KGRKYKSREEYEKAKKKRHPKVAVLVVTDREGNLLFKHVGDKKVKNEQLKKELNKRIT
                     ESNVLCVKPKKEFKKAVKDINSKKVIVNKKQLKRGLYSVEIVESKIDNFKKWLTRFHG
                     VATKYLQSYLMWFVVMNKYLKDIIEPDIGRLLNLSSHDRWAWDKYWKLMKLKYK"
BASE COUNT          619 a          340 c          252 g          794 t
ORIGIN      
        1 atattgctgt tttattacct ggtaaattat tgatgatttt cagcccagat atggtaccat
       61 tacagataat ggaaacaaat ttttatgcta taaaatttat taacaattat ttattgataa
      121 tagaaaatca acgaatatta gagctattac gcttaaaaga ttttatgcca gtatatgtaa
      181 ctagcattag tgaatcaact aaaagtgtag aatgtaataa aaatattgta actactctga
      241 ctgataataa aatttatatt tgggaatata aaaattaaaa aattgcaata acataaacat
      301 ccaatgtttg ttaatttgtc tattgtaatt cggaatttgt ttaaaatttt ctattatata
      361 tgaagcaact ttgtgttatt tttatttggt tctatgacgt ttattgttga aaatataatt
      421 tggataaaat tgtattatca taattaattt aaacaaataa tagattgtaa cgaatactta
      481 taatattaat aagttttttt aaaagttttg agcgaaagag gagaggggtt tgtgaaatta
      541 atattttgtt atacttaatt gctattattt atattttaat ttcattaatt tccaatactt
      601 gtcccacgcc catctgtcgt gcgatgacaa gtttagcaac ctaccaatgt ctggctctat
      661 aatatctttt aggtacttat tcatcactac gaaccacatc aaatagctct gcaagtactt
      721 agtagctacc ccgtgaaatc ttgtcaacca tttcttaaaa ttgtctatct tgctctctac
      781 tatctctaca ctgtataaac ctctctttaa ttgctttttg tttactatta cttttttact
      841 atttatgtct ttaactgcct tcttaaattc ctttttaggc tttacacata gtacattact
      901 ctctgttatc cttttattta gctctttttt caattgctca ttcttaactt ttttatctcc
      961 tacatgtttg aaaagtaaat taccttctct gtctgtaact actaatactg ctaccttggg
     1021 atgtcgcttc ttctttgctt tttcatattc ttctctgctt ttgtatttcc ttcctttttc
     1081 tgaatattgc attagcaatt catctgcctc tactatgcca gagaaactaa tacctccttc
     1141 aaatgatctc aatgctgcta atattctatg cctccatcga aaacttgttt ttaagcaaat
     1201 acctacttca tcagcacaag ctcttaagct tttaccctcg agcatacagc gtaaataatc
     1261 taacatcaag ggatattttc gcatgcgata tacaaaggtg cgtgctgttt cgctgaattg
     1321 gtatctacaa cttttgcatc tatatatctg tctgcctttg cgagtaccag ctttgataac
     1381 gttttcactt ttacagtttg gacagatggc tgctgtagat ttgattttta gcttttgata
     1441 atctactact gtagcatggt catctattag aaattgataa agctgatcga ggaaatcacg
     1501 tttgtgatct ttaggtagag cgaggtattc ttttgaattt tttaataatt tctccataat
     1561 acaaaatttt tgacaaaaat aatacaaata atttaaatgt taaaattttt taagtctaac
     1621 acattttatg ttccatcagc cctcgctcac acttggataa aaccctgttt cacccaatat
     1681 cactattcat aagtctatca ccacctacct ttcattcgca tgaacgaaac atctttagaa
     1741 actttgaaaa aaataaattt ataaacaatt gaattgctat gttttattaa attatcatct
     1801 taaactttgt ctttacccac ttaaaacgtc atagaacctt ttatttattc cacatatgct
     1861 ctaaaatggc tgccttcctt gatataagtg ccagatgtta agattatttt ttctttagca
     1921 tgcatgttta ctttgatgcc cgaatctata atgaatttat tatcagtaat tattggatca
     1981 gcaaatgatt tgtcattttc actat
//
LOCUS       DAOPRN010000131         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_47702_length_1894_cov_115.859163, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000131 DAOPRN010000000
VERSION     DAOPRN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47702_length_1894_cov_115.859163"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(579..1694)
                     /locus_tag="PKI65_08530"
     CDS             complement(579..1694)
                     /locus_tag="PKI65_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HOB78514.1"
                     /translation="MKDLEYRVCQRCVMDNISTPEISFDENGYCNFCNDYVEKISKLT
                     YQGKKSDQKLITILNKIKKNGQNNKYDCLIGLSGGIDSCYTAYLAKKYNLRALLVHMD
                     NGWDTDISIKNIENTVKKLGFDYINYKLDWDEFRDLQLSFLKASVPEIETPTDIAILA
                     VNHMIASQHNIKYIISGGNYATEGILPKSWHYDVKDYKYIKSIHKQYGTKDLHNFPIF
                     DYKKEIYYKFIKGIRIIYILNYIKYDPNEALETLQKEFDWQYYNWKHYESLYTQFVQK
                     YILPAKFNIDYRKTTLSTMICSGLITRDEALEILQEKPYTQTYVDTIKPIICEKLEIS
                     IDEFNDIMQAKPKTYKDFKNSKRKLEFIYKIYRKLFN"
BASE COUNT          675 a          231 c          185 g          803 t
ORIGIN      
        1 aatgattttt tagaattaac aaaagattat ttactattat agattgtgag tagtagtcag
       61 gttgtaatag ataatattac ttgattatta gttttttaat tttcaaaatt aatcaagttt
      121 tcagttggag gctctttttc tcattttttc gtttcgatac gattcttttc acaatgagaa
      181 aaaatcaata acgtggagaa attttacatt tatttatatc tatctgttat taaacgtttt
      241 ataagatcat ttaataattg tataaattaa ttaatgatat tttattaata atttaaaaat
      301 gctaaaaatc attattaatg aatattttaa gaaaatatat ttaaaaattt aatttttata
      361 tattttcttt ttaagctaaa cttagaaaag tattaattgt atttttttga aatttatata
      421 tcattattaa atttaaacat ctataaaaag aaagtatcaa agaaaaatct ccattgaaga
      481 atactgtatt tagtcaagct gataattcat tttgaaattt ttctattaaa taaatatttt
      541 taaaaaatat attctcttaa acgtctattc caattatctt aattaaataa tttcctataa
      601 atcttataaa taaattctaa ttttcgtttt gaatttttga aatctttata cgtttttggt
      661 ttagcttgca taatgtcatt aaactcgtcg atacttattt caagtttttc acaaataatt
      721 ggttttattg tatccacata tgtttgagta taaggttttt cttgtaatat ttccaatgct
      781 tcatctctgg taataaggcc agaacaaatc atagtgctca aagtagtttt acgataatct
      841 atattaaact ttgcaggtaa aatatatttt tgaacaaatt gtgtgtataa tgattcataa
      901 tgtttccaat tgtaatattg ccaatcaaat tctttttgta atgtttctag tgcctcatta
      961 ggatcatatt taatataatt taatatataa ataatcctta tacctttgat aaatttataa
     1021 taaatttcct ttttataatc aaatattggg aaattatgta aatctttggt tccgtattgt
     1081 ttatgtatag atttaatgta tttataatcc ttaacatcat aatgccaact tttgggtaaa
     1141 ataccttcgg tagcataatt acctccgctg ataatatatt taatattatg ttgacttgct
     1201 atcatatgat taacagctaa aatagcgata tctgtaggag tttctatttc aggtacagac
     1261 gcttttaaaa aagataattg caagtctctg aactcgtccc agtccaattt ataatttata
     1321 taatcgaatc ctaatttttt tacagtattt tcaatatttt ttatgctaat atcagtatcc
     1381 caaccattat ccatatgcac taataacgct cttagattat attttttagc taaataagca
     1441 gtatagcaac tatcaatacc accactaaga cctattaaac aatcatattt attattttgt
     1501 ccattttttt taatcttatt taaaattgtt attaattttt ggtcactttt ctttccttga
     1561 tatgttaatt ttgaaatttt ttctacataa tcattacaaa aattacaata accattttca
     1621 tcaaatgaaa tttctggagt tgaaatatta tccattacac atctttggca taccctatac
     1681 tctaggtctt tcattttaca ataattttaa gtgcaaatat aaaaaaatta taataaaaaa
     1741 tagcctttaa taaaatttta tcaaaatatt ttgataaaat gattttttaa ttttttaatc
     1801 tatacctaaa taaattctta ttagattttt tagtcaaact tttgatactc gaataaatat
     1861 ctataaaaac catgcggctt atcaatcaat tctt
//
LOCUS       DAOPRN010000132         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_48812_length_1862_cov_125.526287, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000132 DAOPRN010000000
VERSION     DAOPRN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48812_length_1862_cov_125.526287"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..911
                     /gene="pyrF"
                     /locus_tag="PKI65_08535"
     CDS             78..911
                     /gene="pyrF"
                     /locus_tag="PKI65_08535"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOB78515.1"
                     /translation="MTKKELQSLIFTKKSVLCIGLDADVNKIPHSLVNKYGTTAIEIF
                     NKKVIDATLPYAVAYKFNLAFYEIHGDEGYKMLKNTISYIKSKNERTFLIADGKRNDI
                     GNTAEKYAYAYLTPPLDFDAITINPLMGLDTIEPFLNFHDKWIIPLGLTSNQGAHDFL
                     LEKTKNDEYFFEKIMKTLAQNISSEKMMFVVGATQQPFLQHIRLLLPEYFFLIPGIGT
                     QGGNLEATLKNTLTTQGNILINISRDIIYADKSENFQLKIKEKAKEYQVKMESFLFKN
                     T"
     gene            complement(1363..1470)
                     /gene="rrf"
                     /locus_tag="PKI65_08540"
     rRNA            complement(1363..1470)
                     /gene="rrf"
                     /locus_tag="PKI65_08540"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1544..>1862)
                     /locus_tag="PKI65_08545"
     rRNA            complement(1544..>1862)
                     /locus_tag="PKI65_08545"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          676 a          301 c          264 g          621 t
ORIGIN      
        1 tttgaatgaa ctaatagatg cattgcaagt agccgaaaat actgaaaaat taaaagctga
       61 aatagaataa attttttatg acaaaaaaag aattacaatc cctgatcttt accaaaaaat
      121 cagtcttatg tataggatta gatgcagatg tcaataaaat accacattct ctagttaata
      181 aatatggaac aactgctatt gaaattttta ataaaaaagt aatagatgct actctaccct
      241 atgcagttgc ttataaattt aaccttgctt tttatgaaat acatggtgat gaaggttaca
      301 aaatgctaaa aaatactatc tcctatataa aatcaaaaaa tgaacgaact tttttaatag
      361 ctgatggtaa aagaaatgat ataggcaata cagctgagaa atatgcttac gcatatttga
      421 ctccaccttt agatttcgat gccatcacta tcaatccatt gatgggatta gatacaatag
      481 aaccattttt aaattttcat gataaatgga taataccttt gggacttaca tctaatcagg
      541 gagctcatga ttttttatta gaaaaaacaa aaaatgatga atattttttt gaaaaaatta
      601 tgaaaactct tgcccaaaat attagtagtg aaaaaatgat gttcgtagta ggtgccaccc
      661 aacagccttt tttacaacat atcagattat tattaccaga gtatttcttc ctcatcccag
      721 gtattggtac acaaggtggt aacctcgaag ctactttaaa aaatactttg acaacacaag
      781 gcaatatttt gatcaatatc tctcgcgaca ttatctatgc tgataaatca gaaaattttc
      841 aattaaaaat aaaggaaaaa gcgaaggagt atcaagttaa aatggagagt ttccttttta
      901 aaaacacata aataacatta gattgattca taagttatta tttttaaaaa aatatgcaaa
      961 ataaatttta ctttttattc aattaaatat tttaagtaaa atttaaactt gattattata
     1021 aatacaaaca cttggaaaat ttataccagt ctttttatca tccaaaaaaa taaaaatatc
     1081 tgagtaatat aattgataaa ttagcatata atttagtttt aatgtgttat ttattattaa
     1141 cttataaatt ttacaaatta aatgctaatt tttcgaaatc ataatatttt tatttattat
     1201 tttattgttt taaaatttaa aactacaagt tttttatttt tgtataaatt atttgaatat
     1261 ttatttcatt ttaaataatt tgccacatca cattataaat tatattagaa tgtttaaaca
     1321 ataagaaaaa agtccgcaaa attgcggact tttaaattat ttgtttggtg gcgacctact
     1381 cttccgcgtt agcagtacca tcggcgctgg tgggcttaac gactctgttc ggaatggtaa
     1441 gaggtgaaca ccaccgctat agccaccata actatctaaa aacatacttt gaaggggagg
     1501 attattttga ttttaatagg tttataaaaa atattaatat ctattcgagg aagtatcggg
     1561 taattagtac tgctcggcta aacacattac tatgcttaca cctgcagcct atcaacgtca
     1621 tcgtctctaa cgaccctcta tggaacccta atcttggagt tggcttcgca cttatatgct
     1681 ttcagtgctt atcctatcca tacatagcta ccctgctatg cagttggcac tacaacaggt
     1741 acactagcgg tatgttcatc ccagtcctct cgtactaagg acgactctcc tcaaggttcc
     1801 tacgcccacc gcagataggg accgaactgt ctcgcgacgt tctgaaccca gctcgcgtgc
     1861 ca
//
LOCUS       DAOPRN010000133         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_50272_length_1824_cov_85.655172, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000133 DAOPRN010000000
VERSION     DAOPRN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50272_length_1824_cov_85.655172"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            626..>1824
                     /locus_tag="PKI65_08550"
     CDS             626..>1824
                     /locus_tag="PKI65_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78516.1"
                     /translation="MPKKFNNLQEYISLTREVIKANKDKGNRYAEILSSLYADPVHFL
                     DEIVQNAEDACARIARQGKMIIQFEKDDIAIYHNGDPFNEDDLIAITTYGRSTKKQLS
                     NINKIGKFGIGFRSVFGITDTPEIHSNGFHFVIRDFELLEAIDPIDILQPYTTLFKFP
                     FKKNISALDLWDRVKAWDYRFILLLNYINKIEIINNVTREKFFIEKINTDDSKIVILS
                     TNMTDALADEKYLVLSKINNNQKKLSLALSLEKRYIANPLFVFFPTREILHLPFIVHG
                     FFTTTPTREQIPWDNSAINENKVLLNQLKKLFSNFLKELCNRNLLTPDLYEAFSLSFS
                     EHPIEKLWLEQWIDFLKNNNSILTNDNKYYHHSLVALPRNKLLLDLIPKNVLNKLWNK
                     KNWINIEL"
BASE COUNT          649 a          282 c          213 g          680 t
ORIGIN      
        1 ttgcagagaa tttagctcac attagttggt taaaacagtt caatgataag ctgacgtttc
       61 tgcaagatta aaactattat aatggttttc aaaacatcaa aatattaatt gtttaaattg
      121 attaatgata tatttatttt attttataag catattttat aattataaaa agcaattaaa
      181 acaaattatg cataattaca aaattttaaa aataattcac ttaattatag tactagaatg
      241 atatttatta tattttattt atttattaaa tatcatattt caatttaatc aattaaatat
      301 ttttttaaaa tatgccccct caaatccgct cctacaacct atttacatta aaaaatcatt
      361 ataaatctaa tgtagcttaa acatttttct taaagcttta atcctatcac ttatcactta
      421 tcactcatca cttatcacct atcacttatc acttatcacc tatcacctat tacctctcca
      481 cctcatcacc tcttctcccc ctctcccagt ctcccagtca ctcatcactc atcaactaat
      541 caactgctaa ccccttaaac tcctaattca aaaacttttt acaatatctt ttttttaata
      601 attttgctaa agataaagtt ttgtaatgcc caaaaaattc aataatttac aagaatatat
      661 ttctttgact cgtgaagtaa taaaagctaa taaagataag ggcaatcgct atgcagaaat
      721 tttatcttct ctttatgctg atcctgtaca ttttttggat gaaatagtgc aaaatgctga
      781 ggatgcttgc gctagaattg ccagacaagg caaaatgata atacaatttg agaaagatga
      841 catagctatt tatcataatg gggatccttt caatgaagat gatttaattg ctattactac
      901 ttatgggaga tctactaaaa agcaattgag caatattaat aaaattggaa aatttggtat
      961 tgggtttcgt tctgtatttg gcattactga tactcctgag atacattcta atggctttca
     1021 ttttgttatt cgagattttg aattattaga agctatcgat cctattgata ttctgcaacc
     1081 atatactacc ttatttaagt tcccttttaa aaaaaatatt tctgctttag atttatggga
     1141 tagagtaaaa gcctgggact atagatttat tcttttgctg aattatatta ataaaatcga
     1201 aatcattaat aatgtaactc gcgaaaaatt ttttatagaa aaaatcaata ctgatgatag
     1261 taaaatcgta attttatcta ctaatatgac agacgcttta gctgatgaaa agtatttggt
     1321 ccttagtaaa attaataata atcaaaaaaa attatcttta gctctatcct tagaaaagcg
     1381 atatattgct aatccacttt ttgttttttt ccctactaga gaaatattgc atttgccttt
     1441 tattgtgcat ggttttttta cgaccacacc tactcgcgag cagataccat gggataattc
     1501 agctattaat gagaataaag ttttattgaa tcaactgaaa aaattattct caaattttct
     1561 aaaagagtta tgtaatagaa atttattaac tcccgattta tatgaggctt tttctttatc
     1621 ttttagtgag catcctattg agaaattatg gctcgagcaa tggattgatt ttcttaaaaa
     1681 taataactct attttaacaa atgataataa atattatcat cattctcttg ttgctctccc
     1741 cagaaataaa ttgttgctag atcttatacc taaaaatgtt ttaaataaat tatggaacaa
     1801 aaaaaattgg attaatatag agct
//
LOCUS       DAOPRN010000134         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_52218_length_1773_cov_200.538417, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000134 DAOPRN010000000
VERSION     DAOPRN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52218_length_1773_cov_200.538417"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(469..1002)
                     /locus_tag="PKI65_08555"
     CDS             complement(469..1002)
                     /locus_tag="PKI65_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78517.1"
                     /translation="MVLLSLITIQETFSQTDFIPGYYIINSGATYAIAMPSSLDFFKL
                     DDESYTWSYPDISELYMAAGEVVIAFEYAGGKVYCFDPNGRMVVFNGLNSLTKAPMVP
                     GAGIGYMVETIELIDGSELSEGAYYWIIGQNVANSTIKIQVANGKTYDIPQSKIVLYS
                     TYIKRMMKTETYKTVEE"
BASE COUNT          624 a          266 c          203 g          680 t
ORIGIN      
        1 atattttttc tataacaaac tctagtatcc tattgcaaca aagtggatat aaaccaaaga
       61 aaaattaaga attttgaatt aggcgtttaa gtaataacta aattattact gtgaatgtat
      121 tgataataac tacatttctt atgagtataa ttgattttta aataagtttt tacttaattt
      181 acttttcttt gtcaaccaca caaagaaaag taacaaaaga aaagtctccg caggggaaat
      241 gatttgataa aactacgcaa ttacctcttg gggtttacaa actcgcaagc tttagcttgc
      301 tcaaacagtg taaacccttc tacccaaaac ggcttgcttg ttttattgca aatcatttcc
      361 caatgcggta ttgctagaga ttactaatta ttgcaatgat attcttttat aatacagaat
      421 gttgataaaa actttattag gcaaacgcat atttcactaa gtaaatattt attcttctac
      481 tgtcttataa gtttctgttt tcatcattct ttttatatat gtactatata aaactatttt
      541 agattgcgga atatcataag ttttgccgtt tgcaacttgt atttttattg ttgaattggc
      601 aacattttgt cctataatcc aataataagc cccttctgaa agttcgcttc catctattaa
      661 ttctattgtt tccaccatat atcctatacc tgctcctggt accatagggg cttttgttaa
      721 actattcaga ccattaaaaa ccaccattct tccattaggg tcaaaacaat aaacttttcc
      781 tcctgcatac tcaaaagcaa tgacaacttc accagcagcc atatataatt cactaatatc
      841 tgggtaactc catgtgtaag attcatcgtc taatttaaaa aaatctaaac tgcttggcat
      901 tgctatagca taagtagcgc ccgaatttat aatataatac ccaggtataa aatctgtttg
      961 tgaaaaagtt tcttgaattg ttatcaacga taacaaaacc attaatactg ttgttaactt
     1021 taattttttc ataacttttt attttagggt tgatttgcaa aattataaat attttttaaa
     1081 taaaaaaaat tttaatccat gcccatataa aacgttatgg aaccatattt aacatatcta
     1141 aagtagcaat attataatta aaaaactaaa aaatatagat caaattattt tgttaaaatt
     1201 tttcaaaaaa tttttattga attaggcatt tattaataac taaattatta ctagttcatt
     1261 cacggtaata actatatttc ttataagatt aattgatttc aaataaagtt ctacttaatt
     1321 tacttttctt tgtcaaccac acaaagaaaa gcaccaaaag aaaggtctcc ggtgggtaaa
     1381 tgtgagtagg ggtttaaaat tttaaacctc tacaaaattt aatcttttct taaacggctt
     1441 gcttatttta tggcaaatta tttcccaatg cagatgaaac acatcgtttt ttaaccatac
     1501 atattaatta tgcatagtat ttttgtcaat ataaaataca gataataaat gatttttttc
     1561 ttttttttag gtcaataaaa aattaagttt attaccctac ttttttgtca atacaaattg
     1621 tttattttca ataagttagc taatttcttt ttttaggcca ataaaaaata aagtttatta
     1681 cccatttttt tgtcaatata ataattacta tttgggtaat tctgttttat ttatatttga
     1741 ttataaaagc tttattgcaa tcatttgcta tta
//
LOCUS       DAOPRN010000135         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_53455_length_1743_cov_96.999408, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000135 DAOPRN010000000
VERSION     DAOPRN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53455_length_1743_cov_96.999408"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PKI65_08560"
     CDS             <1..791
                     /locus_tag="PKI65_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78518.1"
                     /translation="WKDNLTLFTTDAKTSRNSSRINAAAAEILVTEAEKTVNPLKKQK
                     LLDDALYYINRTKYIDSDYYAICDISGRIYFASKNYEMALDSYLDCIKKNENPITYNN
                     IFAVGVNAYNDTAVHVAHRAFKFLTEKFPENIDYLYHLSLTYRYMEKFDSAIIILNKI
                     IALDTCHSDAYNQLGEIFSRYYGNFNKGEKYFKKAVECDSTNYSALENLGVLYGIVHR
                     PADALYYFKKAYEIDPYNKNLLRNIGLAYQNLGMQDSANHFLNR"
     gene            complement(815..1492)
                     /locus_tag="PKI65_08565"
     CDS             complement(815..1492)
                     /locus_tag="PKI65_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOB78519.1"
                     /translation="MESKKNSKKDLNRKRTLFLQIGLVMSLTIVFVAFEWKTYEIQEN
                     ILSGINSIQIDQEIVQVTQQEQPEPPKAPQVTTILEVIDGDEIIQDEINISIEDRPDQ
                     AAQTYVPVSIEEAPEPVAEEIFTFVEEYPEFLGGEKALYEYIKNNVNYSELAIKTNIE
                     GTVYVEFVVEKDGSISDVKVLRGIGGGCDEEAVRVVKSMPKWKPGKQRGQPVRVYYTL
                     PIDFKIK"
BASE COUNT          602 a          273 c          236 g          632 t
ORIGIN      
        1 cttggaaaga caatttaacc ttattcacta ctgatgctaa gacctcgcgt aatagctcta
       61 gaataaatgc agcagcagct gaaatcttgg taacagaagc agaaaaaaca gtaaatcctt
      121 tgaaaaaaca gaaattattg gatgatgcat tatattatat aaatagaacc aaatacatag
      181 attctgatta ttacgcaata tgtgatataa gcggtagaat atactttgca agcaaaaatt
      241 atgaaatggc cttagatagc tatctggatt gcatcaaaaa aaatgaaaat ccaattacct
      301 ataataacat attcgctgta ggagtgaatg cttataatga tacagcagtt cacgtagcac
      361 acagagcatt caaattttta acagaaaaat ttccagaaaa tattgattat ttgtatcact
      421 tgtcgttaac atatagatat atggaaaaat ttgattcagc aataataatt ttaaataaaa
      481 ttattgcatt agacacatgc cactcagatg catataatca actaggcgag atattcagta
      541 gatattatgg taactttaat aaaggagaaa aatattttaa aaaagctgtc gaatgtgatt
      601 ctacaaatta ctcagctctt gagaatttag gtgttttata tggtatagtt catagacctg
      661 ctgatgcatt atattatttc aaaaaagctt atgaaattga tccatacaat aaaaatttgt
      721 taagaaatat tggtcttgca tatcaaaatc taggtatgca agatagcgct aatcattttc
      781 ttaataggta aattgcaaca ccactgaact aacattattt tattttaaaa tctattggta
      841 atgtatagta aactctcaca ggttgtcctc tttgtttacc aggtttccat tttggcatag
      901 attttacgac tcttacagct tcttcatcac atcctccccc tatacctctt agtactttca
      961 catcagaaat actaccatct ttttctacaa caaattccac gtatactgtc ccttcaatat
     1021 tagtttttat agctaattca gaatagttaa cattattttt aatatattca taaagtgctt
     1081 tttcaccacc aagaaactca gggtactctt ctacgaaagt aaatatctct tcagctacgg
     1141 gctcaggagc ctcttctatt gaaacaggaa catatgtttg tgccgcttga tcgggtctat
     1201 cttctatact tatattgatc tcatcttgta ttatttcatc accatctatc acctctaata
     1261 ttgtagttac ttgtggtgct ttgggtggct cgggttgctc ttgttgggta acctgaacta
     1321 tttcttgatc tatctgtata gaattaatgc cactcaagat gttttcttgt atttcgtaag
     1381 ttttccattc aaaagctaca aatactatag ttaatgacat aaccaatcct atttgcaaaa
     1441 ataatgttcg cttacgattt aaatcttttt ttgaattttt tttggattcc atgtctacta
     1501 ttcttttatt aaaatatacg taaaaaaagt atttttgtta cattattatt aaagataata
     1561 ttcagaattt atgtatttat atgttgagtc atgtatagtc taatttcaat ttaacatatt
     1621 aacttgaata aggttttaaa aagaaactaa ttgacatcat cctttgtttt actttacgta
     1681 ttttatacta ccattaaata atatttattt aattttacaa aaaaataatt atggataatt
     1741 tta
//
LOCUS       DAOPRN010000136         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_55602_length_1693_cov_103.500611, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000136 DAOPRN010000000
VERSION     DAOPRN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55602_length_1693_cov_103.500611"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PKI65_08570"
     CDS             complement(<1..1130)
                     /locus_tag="PKI65_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78520.1"
                     /translation="MKKTSIILFLMVILVPLVSYSQPSINDPFFDQVSYRGAFDDQDD
                     WTSGWANWDPQNTNYPVTTQTISGEITSNTTWTNDNVYLLQGFVYVVDGVTLTIQPGT
                     IIRGDKNSKGTLIIERGAKLIAQGTASEPIVFTSNQSAGSRNYGDWGGVIICGNAKIN
                     VPGGEAQIEGGPRSYYGGTNDNDNSGILKYLRIEFPGIPFQPDKEINGLTMGGVGKNT
                     TIDYIQVSYSGDDAYEWFGGCVNAKHLITLGSWDDDFDTDYGFSGMIQYAVALREPNI
                     ADVSGSNAFESDNDASGSTNTPQTSAVFSNVSIFGPKKDASTTINSNYKRAMHLRRNT
                     GIDIYNSIFIGWQDGLMIDGSACETNANNNILNIKYTTMAAMA"
BASE COUNT          553 a          293 c          248 g          599 t
ORIGIN      
        1 gccatagcag ccatagtagt gtatttgatg tttagaatgt tattgtttgc attggtttcg
       61 caagcagacc catcaatcat caatccatct tgccaaccta taaaaataga attatatatg
      121 tcaatgccag tatttctgcg aagatgcatt gcacgtttgt aattgctgtt gattgtagta
      181 gaagcatctt ttttaggacc aaaaatagat acatttgaaa aaacagctga agtttgaggt
      241 gtattagtag aaccgctagc gtcattatca ctttcgaaag cattagagcc actcacgtct
      301 gctatattgg gttcacgtag tgctactgca tattgtatca taccagaaaa tccataatct
      361 gtatcaaaat catcatccca gctaccaaga gtaattaaat gtttagcatt cacacaacca
      421 ccaaaccatt cgtaagcatc atcaccacta tatgatacct gtatatagtc aatagtagta
      481 tttttgccta caccacccat agttaaccca ttgatttctt tatcaggttg gaatgggata
      541 ccagggaatt ctattcttaa atattttaaa ataccactat tatcgttatc atttgtgcca
      601 ccataataac tacgtggccc accttctatc tgagcttcac cacctggcac atttattttt
      661 gcattgccac agattatcac tccaccccaa tctccataat ttcgagagcc agcagactga
      721 ttagatgtaa atactattgg ttcactagca gtaccttgag ctattaattt agcaccacgt
      781 tctataatta aagtaccttt gctatttttg tcaccacgaa taatagtacc aggttgtata
      841 gtaagagtaa ctccatctac tacatataca aatccttgta ataaataaac attgtcatta
      901 gtccaagttg tattactagt aatttcacca ctaatagttt gcgtagtaac tggataatta
      961 gtgttttgag gatcccaatt agcccagcca cttgtccaat catcttgatc atcgaaagct
     1021 cctctataac ttacttgatc gaaaaaagga tcattaatac ttggttgaga ataactaact
     1081 aatggcacta atatgaccat tagaaataag ataatacttg tttttttcat aatttttgtt
     1141 ttttgttgtt tctgtattgc aaaattgcaa tacacatatt attcaattat taaatcaatt
     1201 ataaaatttt attaataaat gttaactgaa ttttaaaata aatagatgta taatttaaaa
     1261 ttttttataa aaaattatga ggagggatta gtaaaggagt gatatgacct gttgggaaca
     1321 gatcatatca ctgttaacgt ttgtggttaa tgtgaaatat ttggcggata agattataga
     1381 agacactggg aggttgttga atttggcggc gtcggatagg cgggcatggg agcggtatag
     1441 tgatctgatg tcatcaacat atttaatcta taacatttaa atattttacc aattcccatt
     1501 ttaaacaact cttacaactc attaaacttt ttatacaata aattcccttt tatttttttg
     1561 gcactatctt attaatatta catttgaatt aatcatcata attttttcat cccctttctt
     1621 ctttttatat taatttcagt aattttgcaa ttcaaattaa ttacttatta tggaatataa
     1681 ttttaaagaa att
//
LOCUS       DAOPRN010000137         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_59055_length_1621_cov_188.017241, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000137 DAOPRN010000000
VERSION     DAOPRN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59055_length_1621_cov_188.017241"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            898..>1621
                     /locus_tag="PKI65_08575"
     CDS             898..>1621
                     /locus_tag="PKI65_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78521.1"
                     /translation="MLLLNIISLDYKLKKQNLGYYNQSIAKSQLDNFRKSLDIFIKNI
                     NQIANNNQQREESVKLKIRDFLTSTFYHNDQIDIHGNIDLAILNKENSRNNVEVILEL
                     KTPQNTAEMISTGNLNKKAMWELVHYYMQERLKGNITIKHLIATNGLEWFIFDAREFE
                     DLFYNNKTFYQNYQNWYNDATVNINVQMAYQIIENHIEKSTNMIEAVHFDINAINTNN
                     DDEIIKLYKLLSPQHLLKLPFAN"
BASE COUNT          652 a          205 c          184 g          580 t
ORIGIN      
        1 gtggggcact gagtattttc agaccttgat aatcgactac atcggcttct aattttttca
       61 ttaatgaata aaggttgtct aagaattctt tattctcttc cttactcata gcaaggtatt
      121 tactggtgtt ttctactatt tttttcataa attaaaattt ttgtaaaaat ataaaaatta
      181 tttaacatat aaacttgaaa aacttaaaca tttattaatt tatttattat tgggatgagt
      241 ggaagtttgt ataatatctt cgtgctcttt gtggttaaaa aataacctat ttgtaacaat
      301 ctaaaatttg attacccaca atatccgtta tagaaccgaa attaaatata aataataatc
      361 acacttaatt tgtctataaa aaaaatatct attttttact actttctttt ttgtgttttt
      421 tgttaattta tatatgatta atattaatta atataaaaaa tatatatatt tgtctaattt
      481 atatataaaa tacttgtttt ttaaaaaaat atttgtataa ttgcacaaaa taatcgcaaa
      541 atgataattt gaaaaagatt ttctcatatt tttttctttt ttatagttta aaaaggccac
      601 atattttatg tggctttttt tattagctaa aagttataat actcaaatag aatacaatcc
      661 attatacatt aaaacggaaa aaataattac aaaagaactt agagtataag ctgtagcaag
      721 catctatcct aatatatatt caaaaacaca gagaataata aacatcttca aatatatatt
      781 aattttttta tttataaaac aaataaatat gagtattttg attagttaat aaaatattta
      841 taaatttttt actaaaaatt cgtttaattt taaatatatt tgtaaaaata ataatttatg
      901 ctcctattaa atataatatc actagactat aagctaaaaa aacaaaatct cggatactac
      961 aatcaaagta ttgcaaaatc acaactcgat aatttcagga aatctctaga tatttttatt
     1021 aaaaatatta atcaaatagc gaataataat caacagagag aggagtctgt gaaattaaaa
     1081 attagagatt ttctaacaag caccttctat cataatgatc aaatagatat tcacggcaat
     1141 atagatttag caattctaaa taaagaaaat tcaagaaata atgtagaggt gatactcgaa
     1201 ctcaaaactc cacaaaacac tgctgaaatg atatctacag gcaatctcaa caaaaaagct
     1261 atgtgggagc tggtacatta ttatatgcag gagagactaa aaggcaatat cactatcaag
     1321 catttaatag ctactaatgg gttagaatgg tttatcttcg atgctagaga atttgaagat
     1381 ttattttaca ataataaaac attctatcag aactatcaaa attggtacaa tgatgctact
     1441 gttaatatta atgtacaaat ggcttatcag atcatcgaaa accacattga gaaatctact
     1501 aatatgatag aagcagtaca tttcgatata aatgctataa acacaaataa tgatgatgaa
     1561 atcatcaaac tatacaagct tttatctcca cagcacttgc tcaagttgcc ttttgccaat
     1621 g
//
LOCUS       DAOPRN010000138         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_59905_length_1605_cov_100.469032, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000138 DAOPRN010000000
VERSION     DAOPRN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59905_length_1605_cov_100.469032"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="murF"
                     /locus_tag="PKI65_08580"
     CDS             complement(<1..815)
                     /gene="murF"
                     /locus_tag="PKI65_08580"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOB78522.1"
                     /translation="MTLYLDDIYNIFINSAGISIDSRSTKNGQIFLAIKGENSDGNAY
                     VEDAFNNGASYCITSRTDLLDDSRCIIVEDTYQTLVDLAKIHREHFSIPVLAITGSNG
                     KTTSKELIAKVLSTHYNVLYTEGNLNNELGLPLTILKLNKTHKIAVVEMGAAKLGDIK
                     FLCDIAKPTHGLITNIGRAHIGRFGNFENIIKTKTELFYFLKKHNGLAFINEDDPILK
                     PFAHSLNTIFYGRNNPNTQVSNVSAQPFLSFEWNYNDKNYRIETHLFGDYNLV"
BASE COUNT          624 a          198 c          226 g          557 t
ORIGIN      
        1 tgaactagat tataatctcc aaacaaatga gtctcgattc tgtaattttt atcattataa
       61 ttccattcaa agctaagaaa tggctgagct gaaacattgc tcacttgagt attaggatta
      121 tttctcccat aaaaaatagt atttaaagaa tgggcaaaag gttttagtat tggatcatct
      181 tcattgataa aagcaagacc attgtgtttt ttgagaaaat aaaaaagttc agtttttgtt
      241 ttgataatat tttcgaaatt accaaaacgc ccaatatgtg ctctgccgat attagttatc
      301 aagccatgcg taggtttagc tatatcacaa aggaatttaa tatcacctaa tttagctgct
      361 cccatctcta ctactgctat tttatgagtt ttatttaatt tcaaaatcgt tagaggtaaa
      421 ccaagttcat tattgagatt tccttcagtg tataagacat tgtaatgagt agaaagcact
      481 ttagctatta attctttcga agtagttttg ccattagaac cagtaatagc cagcactgga
      541 atggaaaaat gctctctgtg tattttagct aagtctacta gcgtttggta ggtatcttct
      601 acgatgatgc atctactatc atctaataag tcagttctac tagtaatgca gtacgaagct
      661 ccattattaa aagcatcttc cacataagca ttgccatcac tattttcgcc tttaatagct
      721 agaaatatct gtccattttt tgtagatcta ctatctatgc ttatgcctgc actattaata
      781 aaaatattat aaatatcatc tagatataat gtcattggtt agttaatatt ataaaacaaa
      841 gataagattt ttttttaaaa tttttatttt agtgattttt actatttttg gtatatctaa
      901 ttaattaagc ttaataatag ataataattc aatttaaaaa tatttttata atatatgtaa
      961 tagtaattat taattttgtt atcataaata aaaaaacaat tatttagata aaatgatgaa
     1021 atcattttaa taaaggtaaa aaatatatga ggcttccatt aaagacgtaa tttctaaaaa
     1081 ttggtaaagc aaagacatat accatatcaa atatattttg ccattgtaat aaaaagttag
     1141 tatcattaat aatattactg aaggataaaa aaatcaatgc tatctacata atttattaaa
     1201 aaaagtattt aaatgtaaga ataaatagac ttgtagtatg cttttaaagt gatgcaagaa
     1261 ttaaagataa tcgaagcaat aggagtaagg tggtagcaag agtaggatat catagtgatg
     1321 gagacgagaa ttaaattttt agaaatagac ataaaaaaat tgatgaatta tcaaaattag
     1381 atgtttgcaa aataataaaa acagataaca aaaattaata ttaaaaactg actaagcata
     1441 taaaagcagt aaaaatttaa atacatggag cggacattta gagatatcaa gagcttaata
     1501 acatgcacga atgaaatatg tgaataaaaa tacaaggtaa gattggttag ctaatatatg
     1561 tttcaattaa catatgttat attcactaac tctctatacc gatac
//
LOCUS       DAOPRN010000139         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_61254_length_1580_cov_100.228852, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000139 DAOPRN010000000
VERSION     DAOPRN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61254_length_1580_cov_100.228852"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PKI65_08585"
     CDS             complement(<1..564)
                     /locus_tag="PKI65_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78523.1"
                     /translation="MNKIYILTDYKNIFGSKHNATPYRSGMDKDLLKKYFEIYDFEVI
                     FLKYSDINFRKMNFKDQYVLYTSSEDIGGLYKNYIEDIIYGLHLQGVKLIPDYKYLKA
                     HNNKVFMEILRDLIINDNLNFIHSNYFGTLEELINNINKIELPVVIKSAAGATSRGVY
                     FANNRNELIRNAKKISRSKYIFDELWDL"
     gene            complement(557..781)
                     /locus_tag="PKI65_08590"
     CDS             complement(557..781)
                     /locus_tag="PKI65_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOB78524.1"
                     /translation="MENPANRINTHGSIKNVIIGNNVWIGANCIIMPGVSIGDDSVIA
                     AGSVVIKDIPSKCIVGGNPAKIIKEYNKNE"
     gene            complement(878..1141)
                     /locus_tag="PKI65_08595"
     CDS             complement(878..1141)
                     /locus_tag="PKI65_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78525.1"
                     /translation="MFRKINTIIGFIKINIIYRKHIILRGRVNISGNPILIFSSNAKL
                     ILGNNVTLNSNKKSYHLHMHSPTKIMADKKDAIITIGDNTRIN"
     gene            complement(1126..>1580)
                     /locus_tag="PKI65_08600"
     CDS             complement(1126..>1580)
                     /locus_tag="PKI65_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78526.1"
                     /translation="RYIETKLVWDEVFSFNSIQHSLFGKEMFNSPGNYGGGIMGERML
                     HSDYNVLIHGSGIFGLLFYFYYNVIILKYFLRLKKKFQYKDNTINMMNATFIAVFFMS
                     FIISFSGSFGAVTFNTIRSIYLGAILGIFNNIKFNDNLNKNLNLCLEK"
BASE COUNT          567 a          241 c          161 g          611 t
ORIGIN      
        1 aaggtcccat aattcatcaa atatgtattt tgatctactt attttttttg catttcttat
       61 caattcattt ctattgtttg caaaataaac acctctactc gtagcacctg cagctgattt
      121 aataacgaca ggtaattcaa tcttattaat attatttatt aattcttcta aggtaccaaa
      181 ataattagaa tgaatgaaat taagattatc attaataata agatctctta atatctccat
      241 aaaaacttta ttattatgag cctttagata cttataatca ggaattagtt taactccttg
      301 taaatgtaag ccatatataa tatcctcaat ataattttta taaagtcctc cgatatcctc
      361 agatgatgtg tatagaacat attggtcttt aaagttcatt tttctaaaat taatatcaga
      421 atattttaag aaaataactt caaaatcata tatttcaaaa tattttttta gcaaatcttt
      481 atccattcca cttctataag gagtagcatt atgtttagag ccaaaaatat ttttgtaatc
      541 tgttagaatg taaattttat tcatttttat tatattcttt aattattttt gcaggattac
      601 caccaactat gcatttagat ggtatatcct ttattactac actaccagct gctattacag
      661 aatcgtctcc tatgcttaca ccaggcatta taatacaatt agcacctatc caaacattat
      721 tccctattat aacatttttg atagatccat gtgtatttat tctattagca ggattttcca
      781 tagataagtc atgcccagag cagtcaaata tttctgtatt tgctgcgatt aaacaattag
      841 cacctatttc tatattttta tatgcatgaa tgcaggctca attaattctt gtgttatctc
      901 ctattgtaat tatagcatct tttttgtctg ccattatttt agtgggagaa tgcatatgca
      961 aatggtagct ctttttattt gaatttaaag ttacattatt gcccaatatt aatttagcat
     1021 ttgatgaaaa aattaaaata ggattacctg atatattaac ccttcccctt aatataatgt
     1081 gtttcctata aattatatta attttgataa atccaataat agtatttatt tttctaaaca
     1141 taaatttaaa tttttattta agttatcgtt gaatttaata ttattaaaaa taccaagtat
     1201 agctcccaaa tatattgatc tgattgtatt aaaagttact gcaccgaaac taccagaaaa
     1261 tgaaataata aaagacataa aaaatacagc tataaaagta gcattcatca tattgattgt
     1321 attatcttta tattgaaact tttttttcaa tcttaaaaaa tactttaata ttatcacatt
     1381 gtaataaaaa taaaataata atccaaatat accagaacca tgtataagaa cattataatc
     1441 tgaatgtaac attctttcgc ccattatgcc accaccataa ttacctgggg agttaaacat
     1501 ctctttacca aaaagactat gttgaataga attaaatgaa aaaacttcat cccataccaa
     1561 ttttgtttca atatatctac
//
LOCUS       DAOPRN010000140         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_61432_length_1577_cov_237.261498, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000140 DAOPRN010000000
VERSION     DAOPRN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61432_length_1577_cov_237.261498"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..>1577)
                     /locus_tag="PKI65_08605"
     CDS             complement(41..>1577)
                     /locus_tag="PKI65_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOB78527.1"
                     /translation="KIKPGDKLKIPISKSEIAEYRVSYDTIYHIVKPKETLYSISNQY
                     NVDIEDLRNWNAKILSSGILKINDTIIVGINPKKVDTTSPIVKIQENKIDSSKNINYE
                     VKEGETLYFIAKNFNTSVDSIVKWNQLPSYFVRTGQQLIIKQPIENTAVNKCKKERMP
                     LDVTIAAFVPALDEVTPDMLTSNSNIAVNYISGFDYIQFYEALLLLENMQNDYVTSFN
                     IIPIPQGKNKKEFDKNVSKINWQDVDIVLGPLDEDFLPFTLPLISEKYLWIHYLQKLN
                     VDTMLPEVQFLSNITEELDKVAEYIYKNIKTEIPIYIYHTSDSTEIYNAQYLKNILIN
                     KYGFKNVIFSNTISSLPTKITNQAVVIALTIDEPTAGNIVRHLGTSITNLDFLTFFCP
                     SSWLYFDNLDFYFLNKISPYIMFNNCYIDKNNIEINQFINEFIKTYNTFPSLYAFYAF
                     ETINFLNELISEYGANYKNCITHLDYIGTVCNCNFESIGNAKANKAAKIGRIKDYKLE
                     IVN"
BASE COUNT          533 a          204 c          196 g          644 t
ORIGIN      
        1 tctcccactt aaacattaaa ttataaattg acagtgttta tcaattaact atttctaatt
       61 tataatcctt tatcctacct atcttggcag ctttattagc ttttgcatta cctatactct
      121 caaaattaca attacagact gtacctatat aatctaaatg tgtgatacaa tttttgtagt
      181 tggcaccata ttcacttatc aactcattta aaaaatttat tgtctcaaaa gcatagaaag
      241 catataaaga tggaaaagta ttgtatgttt ttataaattc attaataaat tgatttattt
      301 caatattatt tttgtcaata tagcaattat taaacattat atatggagat attttattta
      361 aaaaataaaa atccaaatta tcaaaatata gccacgaaga tggacagaaa aatgttagaa
      421 aatccaaatt tgtaattgat gttcctaaat gtctaactat attaccagct gtaggttcat
      481 caattgttaa agctattact actgcctggt tcgttatttt cgtgggtagt gatgaaatag
      541 tattagaaaa gattacattt ttaaaaccat atttatttat taaaatattt tttaaatatt
      601 gggcattata tatctctgta gaatctgaag tatggtaaat atatataggt atttctgttt
      661 ttatgttttt ataaatatat tctgccactt tgtctaattc ttcagtgata tttgataaaa
      721 attgcacttc tggtaacata gtatctacat ttaatttttg taaataatgt atccaaagat
      781 atttttcaga aattaatggt aatgtaaaag gtaaaaagtc ttcatctaat ggacctagta
      841 ctatatctac gtcttgccaa ttaatttttg aaacattttt gtcaaattct tttttatttt
      901 tcccttgtgg aattggtatt atattaaaag aagttacata atcattttgc atattttcta
      961 gtaataataa tgcttcataa aattgaatat agtcaaatcc actaatgtaa ttgactgcaa
     1021 tattagaatt tgatgttagc atatcaggag taacctcgtc taaagcaggt acaaaagcag
     1081 caatggtaac atctaatggc atgcgttctt ttttgcattt atttactgct gtattttcta
     1141 ttggttgttt tataatcaac tgttgtccag ttcttacgaa atatgatggt agctgattcc
     1201 atttaactat gctatctaca gaggtattaa agttttttgc aatgaaatat aaagtttctc
     1261 cttctttaac ttcataatta atattttttg agctatcaat tttattctct tgtattttaa
     1321 ctataggaga tgtagtgtca acctttttgg gatttatacc tacaattata gtatcattaa
     1381 tttttaatat gccagatgat aaaatttttg cattccaatt gcgtaaatct tctatatcta
     1441 cattatattg attactaatg ctatataatg tttctttagg tttcacaata tgataaatgg
     1501 tatcatatga aactctatat tcagcaattt ctgatttaga aataggaatt tttaacttat
     1561 caccaggttt tattttt
//
LOCUS       DAOPRN010000141         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1495
            NODE_65152_length_1513_cov_111.620027, whole genome shotgun
            sequence.
ACCESSION   DAOPRN010000141 DAOPRN010000000
VERSION     DAOPRN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854700
            Sequence Read Archive: SRR4236663
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:17:24
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,723
            CDSs (total)                      :: 1,678
            Genes (coding)                    :: 1,669
            CDSs (with protein)               :: 1,669
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 1 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65152_length_1513_cov_111.620027"
                     /isolate="AMR_MDS_1495"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="37.8 N 122.27 W"
                     /collection_date="2015-02-18"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PKI65_08610"
     CDS             <1..263
                     /locus_tag="PKI65_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOB78528.1"
                     /translation="FFPAYKLSNLPPTPVETIMAAVKIARDNGMRYVYTGNVLDVNND
                     STFCPQCNSILIKRQGYHTSIIGLNSEGNCTKCGYHIPGVWS"
     gene            364..>1513
                     /locus_tag="PKI65_08615"
     CDS             364..>1513
                     /locus_tag="PKI65_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOB78529.1"
                     /translation="MEKFLFFILLMISMVAMSQNTVINKNFDDNNFLHSNDIDSTLHL
                     ITPYFSGLASEKNYELLVGANFKKEFQNKNTFFNIWTKPEFLLFSLYDYLDQYDSIFR
                     FPGGEKIYNGHYSWNAPIELTMNVKNHYFLSAGYSKFHWGDGYRGLNLSYASANYPYL
                     LQQFKIKGLEFSWAIMKWTNPLTELKYDKYSIFHSFSFNIGQKWRFAFFENLIYPARD
                     STHNNFEFVYLLPQIFYRYVDYSLGSPYNLIFGGNFNWHAAKNFQIYGQAILDDFIFN
                     EFQKDIKHRLHPDDTTIVYGNWMNKYAIQIGFKWRTNNNNFHSRLEFNAARPYTYSHK
                     NPIQNFSNNGQPVAHPFGANFYEALFEASLIKKNWQFYFISFYKHIGYD"
BASE COUNT          510 a          211 c          226 g          566 t
ORIGIN      
        1 gatttttccc tgcatataaa ttatctaatt taccacctac acctgtagaa actattatgg
       61 ctgctgtcaa aatagctcgt gataatggta tgagatatgt ttacactggt aatgttttag
      121 acgtaaataa tgatagtact ttttgccctc aatgcaattc tattttgata aaacgtcaag
      181 gttatcatac atctatcata ggtttaaata gcgaaggaaa ttgcacgaaa tgtggttatc
      241 atatccctgg tgtttggtca tagacattgt attttctaaa atataaacat tagtattttt
      301 tctattaatt tataatttta agtttaacta tttttgtaca aaaaaagtta aacttaattt
      361 ttaatggaga aatttttatt ttttatttta ctgatgataa gcatggtagc aatgtcacaa
      421 aatacagtta taaataaaaa ttttgatgac aataattttt tacatagtaa tgatattgat
      481 agtacactac atttgatcac tccttatttt agtggattag caagtgaaaa aaattatgaa
      541 ttattggtcg gtgctaattt caaaaaggag tttcaaaaca aaaatacttt tttcaacatt
      601 tggacaaaac ctgagttttt attattttct ttgtatgatt atttggacca atatgattct
      661 atctttcgtt tccctggtgg agaaaagata tataatggac attattcatg gaatgcccct
      721 atagagctaa caatgaatgt taaaaatcat tatttcttat ctgcaggcta tagtaaattt
      781 cattggggcg atggttatag aggattgaat ttatcatatg ccagtgctaa ttatccttat
      841 ttgcttcaac aatttaaaat caaaggatta gaattttcct gggctatcat gaagtggact
      901 aatccactga cagaacttaa atatgataaa tattccatat ttcacagctt ttcatttaat
      961 atcgggcaaa aatggcgttt tgcatttttc gaaaatctta tttatccagc gagagatagt
     1021 actcataata atttcgagtt tgtttattta ttgcctcaga ttttttacag atatgtagat
     1081 tattcacttg gctcacctta taatttaatt tttggtggca attttaattg gcatgcagct
     1141 aaaaacttcc aaatttatgg acaagctatt ttagatgatt tcatatttaa tgaatttcaa
     1201 aaagatataa agcatagatt gcatcctgat gataccacca tcgtttatgg taattggatg
     1261 aataaatatg ccatacaaat aggtttcaag tggagaacaa acaataataa tttccactcg
     1321 agattagaat ttaatgctgc tcgtccttat acttatagtc acaaaaatcc tatacaaaat
     1381 ttctcgaata atggtcagcc tgtagctcat ccttttggag ctaattttta tgaagctttg
     1441 ttcgaggctt cactaattaa aaaaaattgg caattttatt tcataagttt ctataaacac
     1501 attggctatg act
//
